deoxyribonuclease hpaii


Summary: One of the Type II site-specific deoxyribonucleases (EC It recognizes and cleaves the sequences C/CGG and GGC/C at the slash. HpaII is from Haemophilus parainfluenzae. Several isoschizomers have been identified. EC 3.1.21.-.

Top Publications

  1. Stratil A, Kopecny M, Moser G, Schröffel J, Cepica S. HpaII and RsaI PCR-RFLPs within an intron of the porcine leptin receptor gene (LEPR) and its linkage mapping. Anim Genet. 1998;29:405-6 pubmed
  2. Kawajiri K, Nakachi K, Imai K, Yoshii A, Shinoda N, Watanabe J. Identification of genetically high risk individuals to lung cancer by DNA polymorphisms of the cytochrome P450IA1 gene. FEBS Lett. 1990;263:131-3 pubmed
    ..This is the first report to identify the genetically high risk individuals to lung cancer at the gene level. ..
  3. Hatada I, Fukasawa M, Kimura M, Morita S, Yamada K, Yoshikawa T, et al. Genome-wide profiling of promoter methylation in human. Oncogene. 2006;25:3059-64 pubmed
    ..Genome-wide analysis of hypomethylated promoter sequences in cancer demonstrated low CG/GC ratio of these sequences, suggesting that CpG-poor genes are sensitive to demethylation activity in cancer. ..
  4. Ichiyanagi K. Inhibition of MspI cleavage activity by hydroxymethylation of the CpG site: a concern for DNA modification studies using restriction endonucleases. Epigenetics. 2012;7:131-6 pubmed publisher
    ..Therefore, DNA modification studies that use MspI, for example, reduced representation bisulfite shotgun sequencing, quantitative analysis of 5-hmC, and cleavage-sensitivity analysis, should be carefully interpreted. ..
  5. Yegnasubramanian S, Lin X, Haffner M, DeMarzo A, Nelson W. Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation. Nucleic Acids Res. 2006;34:e19 pubmed
    ..This novel technology could significantly improve our ability to detect CGI hypermethylation. ..
  6. Singer Sam J, LeBon J, Tanguay R, Riggs A. A quantitative HpaII-PCR assay to measure methylation of DNA from a small number of cells. Nucleic Acids Res. 1990;18:687 pubmed
  7. Feinberg A, Vogelstein B. Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature. 1983;301:89-92 pubmed
    ..This hypomethylation was progressive in a metastasis from one of the patients. ..
  8. Xu Q, Kucera R, Roberts R, Guo H. An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site. Structure. 2004;12:1741-7 pubmed
    ..A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer. ..
  9. Mello M, Russo P, Russo J, Vidal B. Entropy of Feulgen-stained 17-beta-estradiol-transformed human breast epithelial cells as assessed by restriction enzymes and image analysis. Oncol Rep. 2009;21:1483-7 pubmed
    ..Thus, we assume that a variable epigenetic modulation affecting the higher-order packing states of chromatin in the estrogen-transformed MCF-10F cell model could be evident with the chromatin entropy study by image analysis. ..

More Information


  1. Tryndyak V, Kovalchuk O, Pogribny I. Identification of differentially methylated sites within unmethylated DNA domains in normal and cancer cells. Anal Biochem. 2006;356:202-7 pubmed
  2. Hentzen D, Pelet A, Feldman D, Rabier D, Berthelot J, Munnich A. Fatal hyperammonemia resulting from a C-to-T mutation at a MspI site of the ornithine transcarbamylase gene. Hum Genet. 1991;88:153-6 pubmed
    ..8-kb abnormal fragment should be investigated on Southern blots of affected individuals. ..
  3. Brown L, Alonso M, Yu J, Warburton D, Brown S. Prenatal diagnosis of a familial Xq deletion in a female fetus: a case report. Prenat Diagn. 2001;21:27-30 pubmed
    ..The pregnancy resulted in the birth of a normal girl, and X chromosome inactivation skewing was demonstrated in both mother and daughter. Xq deletion phenotypes and counseling issues are reviewed. ..
  4. Fontana X, Peyrottes I, Rossi C, Leblanc Talent P, Ettore F, Namer M, et al. Study of the frequencies of CYP1A1 gene polymorphisms and glutathione S-transferase mu1 gene in primary breast cancers: an update with an additional 114 cases. Mutat Res. 1998;403:45-53 pubmed
    ..6.4% for m1 (-/+) or (+/+) genotype and copy number of GSTM1 superior to one (p < 0.0000). The CYP1A1 gene wild form seems to be associated with early cancer development in Caucasian patients. ..
  5. Kia E, Rahimi H, Sharbatkhori M, Talebi A, Fasihi Harandi M, Mirhendi H. Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitol Res. 2010;107:757-60 pubmed publisher
  6. Zeldenrust S, Murrell J, Farlow M, Ghetti B, Roses A, Benson M. RFLP analysis for APP 717 mutations associated with Alzheimer's disease. J Med Genet. 1993;30:476-8 pubmed
    ..In addition, DNA from 145 FAD subjects were tested for the three known APP 717 mutations. ..
  7. Ji Y, Wang Q, Lin X, Suo L. CYP1A1 MspI polymorphisms and lung cancer risk: an updated meta-analysis involving 20,209 subjects. Cytokine. 2012;59:324-34 pubmed publisher
    ..However, the associations vary in different ethnic populations, histological types of lung cancer and the gender of case and control populations. ..
  8. Peng D, He Y, Qiu L, Yang F, Lin J. [Susceptibility to endometriosis in women of Han Nationality in Guangdong Province associated with Msp I polymorphisms of cytochrome P450 1A1 gene]. Di Yi Jun Yi Da Xue Xue Bao. 2002;22:814-6 pubmed
    ..Msp I polymorphisms of cytochrome P4501A1 in itself might not be associated with the susceptibility to endometriosis in women of Han Nationality in Guangdong Province. ..
  9. Zhuo W, Zhang L, Wang Y, Zhu B, Chen Z. CYP1A1 MspI polymorphism and acute myeloid leukemia risk: meta-analyses based on 5018 subjects. J Exp Clin Cancer Res. 2012;31:62 pubmed publisher
    ..The results of the present study suggested that CYP1A1 MspI polymorphism might be a risk factor for AML among Asians. Further investigations are needed to confirm the conclusions. ..
  10. Kato N, Ikeda M, Sugiyama K, Mizutani T, Tanaka T, Shimotohno K. Hepatitis C virus population dynamics in human lymphocytes and hepatocytes infected in vitro. J Gen Virol. 1998;79 ( Pt 8):1859-69 pubmed
    ..Following a comparison of the sequences, 11 amino acids were identified as candidates for determinants of the cell tropism of HCV. ..
  11. Soumillion A, Erkens J, Lenstra J, Rettenberger G, te Pas M. Genetic variation in the porcine myogenin gene locus. Mamm Genome. 1997;8:564-8 pubmed
    ..The PCR-RFLP tests and microsatellite markers on Chr 9 offer the possibility to genotype large numbers of pigs for studies of genetic linkage to meat deposition and growth characteristics. ..
  12. Crew E, Yan H, Lin L, Yin J, Skeete Z, Kotlyar T, et al. DNA assembly and enzymatic cutting in solutions: a gold nanoparticle based SERS detection strategy. Analyst. 2013;138:4941-9 pubmed publisher
  13. Hoogendijk J, Hensels G, Zorn I, Valentijn L, Janssen E, de Visser M, et al. The duplication in Charcot-Marie-Tooth disease type 1a spans at least 1100 kb on chromosome 17p11.2. Hum Genet. 1991;88:215-8 pubmed
    ..Using pulsed-field gel electrophoresis analysis, we estimated the minimal size of the duplicated region in CMT1a patients to be 1100 kb. ..
  14. Shawky R, Sayed N, Elhawary N. Mutations in transglutaminase 1 gene in autosomal recessive congenital ichthyosis in Egyptian families. Dis Markers. 2004;20:325-32 pubmed
    ..We clearly demonstrated that the ARCI Egyptian families in Upper Egypt was ethnically pure and had a tendency not to be a hybrid with other populations in Lower Egypt, Delta zone and Cairo city. ..
  15. Khodosevich K, Lebedev I, Sverdlov E. [The tissue-specific methylation of human-specific endogenous retroviral long terminal repeats]. Bioorg Khim. 2004;30:493-8 pubmed
    ..The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // ..
  16. Ahmad S, Mokaddas E. Contribution of AGC to ACC and other mutations at codon 315 of the katG gene in isoniazid-resistant Mycobacterium tuberculosis isolates from the Middle East. Int J Antimicrob Agents. 2004;23:473-9 pubmed
    ..tuberculosis isolates recovered from Middle Eastern patients and should be incorporated in a rapid screen for the detection of mutations for isoniazid-resistance in the katG gene from this ethnic group. ..
  17. De Brasi C, Rossetti L, Larripa I. Rapid genotyping of XbaI and MspI DNA polymorphisms of the human factor VIII gene: estimation of their combined heterozygosity in the Argentinean population. Haematologica. 2003;88:232-4 pubmed
  18. Shiffrin N, Gruber J, Glatt S, Faraone S. No association between MspI allele of the ADRA2A polymorphism and ADHD: meta-analysis of family-based studies. Psychiatr Genet. 2013;23:174-5 pubmed publisher
    ..The present investigation reports results from a meta-analysis of family-based studies that did not find a significant association between the MspI polymorphism of the ADRA2A gene and ADHD. ..
  19. Nicolson N, Nicolson G. Nucleoprotein gene tracking: localization of specific HIV-1 genes to subchromatin nucleoprotein complexes containing endonuclease activity in HIV-1-infected human cells. J Cell Biochem. 1999;Suppl 32-33:158-65 pubmed
    ..The technique can be used to study a variety of problems concerning the association of specific genes and enzymes with specific nucleoprotein complexes J. Cell. Biochem. Suppls. 32/33:158-165, 1999. ..
  20. Sengupta B, Friedberg F, Detera Wadleigh S. Molecular analysis of human and rat calmodulin complementary DNA clones. Evidence for additional active genes in these species. J Biol Chem. 1987;262:16663-70 pubmed
    ..Transcripts of lambda rCB1 and lambda hCE1 were observed in all tissues examined indicating the absence of tissue-specific expression. Calmodulin gene polymorphisms were detected using TaqI, HindIII, and MspI. ..
  21. Salier J, Verga V, Doly J, Diarra Mehrpour M, Erickson R. The genes for the inter-alpha-inhibitor family share a homologous organization in human and mouse. Mamm Genome. 1992;2:233-9 pubmed
    ..Therefore, a similar number and arrangement of I alpha I genes is found in mouse and human, including the triplication of an H gene ancestor. These results point to an ancient origin of this complex set of genes. ..
  22. Griffon N, Crocq M, Pilon C, Martres M, Mayerova A, Uyanik G, et al. Dopamine D3 receptor gene: organization, transcript variants, and polymorphism associated with schizophrenia. Am J Med Genet. 1996;67:63-70 pubmed
    ..16, df = 1, P = 0.69, respectively). The large distance of the Msp I polymorphism from the Bal I polymorphism and its localization in the 3' part of the gene may explain the discrepant results obtained with the two polymorphisms. ..
  23. Naso M, Morgan J, Buchberg A, Siracusa L, Iozzo R. Expression pattern and mapping of the murine versican gene (Cspg2) to chromosome 13. Genomics. 1995;29:297-300 pubmed
    ..Using interspecific backcross analysis, we assigned the versican gene (Cspg2) to mouse chromosome 13, in a region that is syntenic with the long arm of human chromosome 5 where the human CSPG2 gene is located. ..
  24. Quinn G, Wood J, Suling K, Arn S, Sachs D, Schuurman H, et al. Genotyping of porcine endogenous retroviruses from a family of miniature swine. J Virol. 2004;78:314-9 pubmed
  25. Tsao Y, Wei C, Robberson D, Gotto A, Chan L. Isolation and characterization of the human apolipoprotein A-II gene. Electron microscopic analysis of RNA:DNA hybrids, nucleotide sequence, identification of a polymorphic MspI site, and general structural organization of apolipoprotein genes. J Biol Chem. 1985;260:15222-31 pubmed
  26. Blackall P, Fegan N, Chew G, Hampson D. Population structure and diversity of avian isolates of Pasteurella multocida from Australia. Microbiology. 1998;144 ( Pt 2):279-89 pubmed
    ..Both the MLEE results and the ribotyping data identified a previously unrecognized subset of P. multocida strains...
  27. Ben Hattar J, Jiricny J. Effect of cytosine methylation on the cleavage of oligonucleotide duplexes with restriction endonucleases HpaII and MspI. Nucleic Acids Res. 1988;16:4160 pubmed
  28. Hayashi H, Takahashi R, Nishi T, Sakamoto M, Benno Y. Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphism. J Med Microbiol. 2005;54:1093-101 pubmed
    ..The results showed marked individual differences in the composition of microbiota in each region. ..
  29. McDonald P, Lewis M, Murphy B, O REILLY R, Singh S. Appraisal of genetic and epigenetic congruity of a monozygotic twin pair discordant for schizophrenia. J Med Genet. 2003;40:E16 pubmed
  30. Chang J, Liu H, Shih M, Liu S, Chan W, Tsai F. Unstable Hb Perth in a Taiwanese subject: a T-->C substitution at codon 32 of the beta-globin gene creates an MspI site. Hemoglobin. 2002;26:91-4 pubmed
  31. Valli M, Sangalli A, Rossi A, Mottes M, Forlino A, Tenni R, et al. Osteogenesis imperfecta and type-I collagen mutations. A lethal variant caused by a Gly910-->Ala substitution in the alpha 1 (I) chain. Eur J Biochem. 1993;211:415-9 pubmed
    ..Restriction analysis, along with the biochemical screening of collagens, allowed us to perform prenatal diagnosis on cells from chorionic-villus sampling and to exclude the recurrence of the mutation in the sibling. ..
  32. Vianna M, Conrads G, Gomes B, Horz H. T-RFLP-based mcrA gene analysis of methanogenic archaea in association with oral infections and evidence of a novel Methanobrevibacter phylotype. Oral Microbiol Immunol. 2009;24:417-22 pubmed publisher
    ..Here we assessed the prevalence and distribution of methanogens and possible associations with bacteria in oral biofilms...
  33. Wetmur J, Kaya A, Plewinska M, Desnick R. Molecular characterization of the human delta-aminolevulinate dehydratase 2 (ALAD2) allele: implications for molecular screening of individuals for genetic susceptibility to lead poisoning. Am J Hum Genet. 1991;49:757-63 pubmed
    ..abstract truncated at 250 words) ..
  34. Tang K, Li S, Yang W, Yu J, Han L, Li X, et al. An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows. Mol Biol Rep. 2011;38:17-21 pubmed publisher
    ..These results indicate that INHA gene can be used as a predictor for superovulation in Chinese Holstein cows, and imply that cows with AA genotype should be excluded for superovulation practices. ..
  35. Thunberg U, Gidlof C, Bånghagen M, Sällström J, Sundstrom C, Totterman T. HpaII polymerase chain reaction restriction fragment length polymorphism in the human CD19 gene on 16p11. Hum Hered. 1998;48:230-1 pubmed
  36. Sasaki H, Jones P, Chaillet J, Ferguson Smith A, Barton S, Reik W, et al. Parental imprinting: potentially active chromatin of the repressed maternal allele of the mouse insulin-like growth factor II (Igf2) gene. Genes Dev. 1992;6:1843-56 pubmed
    ..The differential activity of the parental Igf2 alleles could be achieved through epigenetic modifications situated outside the promoters or by subtle and yet unidentified modifications at the promoters. ..
  37. Hadfield R, Manek S, Weeks D, Mardon H, Barlow D, Kennedy S. Linkage and association studies of the relationship between endometriosis and genes encoding the detoxification enzymes GSTM1, GSTT1 and CYP1A1. Mol Hum Reprod. 2001;7:1073-8 pubmed
    ..However, there was no evidence of linkage, suggesting that this region may not be implicated in disease susceptibility. ..
  38. Krzecio E, Kurył J, Koćwin Podsiadła M, Monin G. Association of calpastatin (CAST/MspI) polymorphism with meat quality parameters of fatteners and its interaction with RYR1 genotypes. J Anim Breed Genet. 2005;122:251-8 pubmed
    ..Significant interactions between CAST and RYR1 genotypes observed indicate that the quality of meat influenced by RYR1 genotype may be modified by the simultaneous influence of genotype as regards the CAST locus. ..
  39. Hayashi S, Watanabe J, Nakachi K, Kawajiri K. Genetic linkage of lung cancer-associated MspI polymorphisms with amino acid replacement in the heme binding region of the human cytochrome P450IA1 gene. J Biochem. 1991;110:407-11 pubmed
  40. Vafiadis P, Ounissi Benkalha H, Palumbo M, Grabs R, Rousseau M, Goodyer C, et al. Class III alleles of the variable number of tandem repeat insulin polymorphism associated with silencing of thymic insulin predispose to type 1 diabetes. J Clin Endocrinol Metab. 2001;86:3705-10 pubmed
  41. Gomes M, Gomes C, Pinto W, Ramos E. Methylation pattern at the KvDMR in a child with Beckwith-Wiedemann syndrome conceived by ICSI. Am J Med Genet A. 2007;143A:625-9 pubmed
  42. Cavalli S, Hirata M, Hirata R. Rapid detection of 3500Q and 3531 mutations and MspI polymorphism in exon 26 at the apolipoprotein B gene. J Clin Lab Anal. 2001;15:35-9 pubmed
    ..In conclusion, this procedure is simple and rapid, being easily introduced in clinical laboratories for direct detection of the more frequent mutations at the apo B gene associated with hypercholesterolemia. ..
  43. Matsuo K, Tang S, Zeki K, Gutman R, Fagin J. Aberrant deoxyribonucleic acid methylation in human thyroid tumors. J Clin Endocrinol Metab. 1993;77:991-5 pubmed
    ..This suggests that widespread changes in DNA methylation may occur as a relatively early step in thyroid tumor formation. ..
  44. Lavia P, Macleod D, Bird A. Coincident start sites for divergent transcripts at a randomly selected CpG-rich island of mouse. EMBO J. 1987;6:2773-9 pubmed
    ..The results support the view that HTF islands often mark genes, and they suggest that bidirectional transcription may be a common feature of island promoters. ..
  45. Uematsu F, Kikuchi H, Abe T, Motomiya M, Ohmachi T, Sagami I, et al. MspI polymorphism of the human CYP2E gene. Nucleic Acids Res. 1991;19:5797 pubmed
  46. Maffei E, Pompolo S. Evaluation of hot saline solution and restriction endonuclease techniques in cytogenetic studies of Cycloneda sanguinea L. (Coccinellidae). Genet Mol Res. 2007;6:122-6 pubmed
  47. Bonne G, Di Barletta M, Varnous S, Becane H, Hammouda E, Merlini L, et al. Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy. Nat Genet. 1999;21:285-8 pubmed
    ..Together with mutations in EMD (refs 5,6), they underscore the potential importance of the nuclear envelope components in the pathogenesis of neuromuscular disorders...
  48. Knebelmann B, Deschenes G, Gros F, Hors M, Grunfeld J, Zhou J, et al. Substitution of arginine for glycine 325 in the collagen alpha 5 (IV) chain associated with X-linked Alport syndrome: characterization of the mutation by direct sequencing of PCR-amplified lymphoblast cDNA fragments. Am J Hum Genet. 1992;51:135-42 pubmed
    ..This gly325-to-arg substitution presumably alters the triple-helix formation, and, in turn, modifies the ultrastructural and functional characteristics of the type IV collagen network inside the glomerular basement membrane...
  49. Aquilanti L, Mannazzu I, Papa R, Cavalca L, Clementi F. Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae: a contribution to the study of these free-living nitrogen-fixing bacteria. J Microbiol Methods. 2004;57:197-206 pubmed
    ..Cluster analysis was successfully employed for the identification of members of the family Azotobacteraceae, being assignation into species of the isolates confirmed by means of partial 16S rRNA gene sequencing...
  50. Comings D, Johnson J, Gonzalez N, Huss M, Saucier G, McGue M, et al. Association between the adrenergic alpha 2A receptor gene (ADRA2A) and measures of irritability, hostility, impulsivity and memory in normal subjects. Psychiatr Genet. 2000;10:39-42 pubmed
    ..The ADRA2A gene accounted for 1.8-8.3% of the variance of these scores. ..
  51. Shenker A, Laue L, Kosugi S, Merendino J, Minegishi T, Cutler G. A constitutively activating mutation of the luteinizing hormone receptor in familial male precocious puberty. Nature. 1993;365:652-4 pubmed
    ..COS-7 cells expressing the mutant LH receptor exhibited markedly increased cyclic AMP production in the absence of agonist, suggesting that autonomous Leydig cell activity in FMPP is caused by a constitutively activated LH receptor. ..
  52. Onaran M, Yilmaz A, Sen I, Ergun M, Camtosun A, Kupeli B, et al. A HindIII polymorphism of fibronectin gene is associated with nephrolithiasis. Urology. 2009;74:1004-7 pubmed publisher
    ..This association makes FN a good candidate for further studies about the etiology of stone disease, and in the future it could be a candidate marker for evaluating the genetic risks in patients with nephrolithiasis. ..
  53. Lebo R, Anderson L, DiMauro S, Lynch E, Hwang P, Fletterick R. Rare McArdle disease locus polymorphic site on 11q13 contains CpG sequence. Hum Genet. 1990;86:17-24 pubmed
    ..Fluorescence in situ hybridization sublocalized this gene to proximal band 11q13, establishing a point of cross-reference between the physical and genetic maps. ..