dna ligase atp


Summary: ATP-dependent cellular enzyme which catalyzes DNA replication, repair and recombination through formation of internucleotide ester bonds between phosphate and deoxyribose moieties. Vertebrate cells encode three well-characterized DNA ligases, DNA ligase I, III and IV, all of which are related in structure and sequence. DNA ligases either require ATP or NAD. However, archaebacterial, viral, and some eubacterial DNA ligases are ATP-dependent.

Top Publications

  1. Giroux X, MacNeill S. Inhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii. FEMS Microbiol Lett. 2015;362: pubmed publisher
    ..CPOdA has the potential to become a vital tool in DNA replication and repair studies in this important model organism. ..
  2. Allen S, Hug I, Pabian S, Rzeszutek I, Hoehener C, Nowacki M. Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs. Cell. 2017;168:990-999.e7 pubmed publisher
    ..This process allows the generation of a double-stranded RNA for Dicer-like protein cleavage to give rise to a population of small regulatory RNAs that precisely match the excised DNA sequences. VIDEO ABSTRACT. ..
  3. Aceytuno R, Piett C, Havali Shahriari Z, Edwards R, Rey M, Ye R, et al. Structural and functional characterization of the PNKP-XRCC4-LigIV DNA repair complex. Nucleic Acids Res. 2017;45:6238-6251 pubmed publisher
    ..Together, this work unveils multipoint contacts between PNKP and XRCC4-LigIV that regulate PNKP recruitment and activity within NHEJ. ..
  4. Ribeiro H, Maia A, de Oliveira R, Costa M, Farias I, de Paula Borges D, et al. DNA repair gene expressions are related to bone marrow cellularity in myelodysplastic syndrome. J Clin Pathol. 2017;70:970-980 pubmed publisher
    ..These correlations demonstrate the important intrinsic relations between single and double DNA strand breaks genes in MDS, emphasising that these genes are related to MDS pathogenesis. ..
  5. McNally J, O Brien P. Kinetic analyses of single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I. J Biol Chem. 2017;292:15870-15879 pubmed publisher
    ..The biochemical differences between the LIG3 isoforms and LIG1 identified here will guide the understanding of both unique and overlapping biological roles of these critical enzymes. ..
  6. Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, et al. Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation. Mol Cell. 2017;67:550-565.e5 pubmed publisher
    ..These results further elucidate the function of UHRF1, identify a non-histone target of G9a and GLP, and provide an example of a histone mimic that coordinates DNA replication and DNA methylation maintenance. ..
  7. Wang X, Sun C, Hageman L, Smith K, Singh P, Desai S, et al. Clinical and Genetic Risk Prediction of Subsequent CNS Tumors in Survivors of Childhood Cancer: A Report From the COG ALTE03N1 Study. J Clin Oncol. 2017;35:3688-3696 pubmed publisher
    ..This information can be used to inform surveillance for early detection of subsequent CNS tumors. ..
  8. Chiruvella K, Renard B, Birkeland S, Sunder S, Liang Z, Wilson T. Yeast DNA ligase IV mutations reveal a nonhomologous end joining function of BRCT1 distinct from XRCC4/Lif1 binding. DNA Repair (Amst). 2014;24:37-45 pubmed publisher
    ..Together, these separation-of-function mutants indicate that Dnl4 BRCT1 supports DSB recruitment and NHEJ in a manner distinct from Lif1 binding and reveal a complexity of Dnl4 BRCT domain functions in support of stable DSB association. ..
  9. Henríquez Hernández L, Valenciano A, Foro Arnalot P, Álvarez Cubero M, Cozar J, Suárez Novo J, et al. Single nucleotide polymorphisms in DNA repair genes as risk factors associated to prostate cancer progression. BMC Med Genet. 2014;15:143 pubmed publisher
    ..57 (CI 95% 1.28 - 5.16)). Genetic variants at DNA repair genes are associated with prostate cancer progression, and would be taken into account when assessing the malignancy of prostate cancer. ..

More Information


  1. Zelensky A, Schimmel J, Kool H, Kanaar R, Tijsterman M. Inactivation of Pol ? and C-NHEJ eliminates off-target integration of exogenous DNA. Nat Commun. 2017;8:66 pubmed publisher
    ..Here the authors show that repression of polymerase ? and classical non-homologous recombination eliminates random integration. ..
  2. Gerodimos C, Chang H, Watanabe G, Lieber M. Effects of DNA end configuration on XRCC4-DNA ligase IV and its stimulation of Artemis activity. J Biol Chem. 2017;292:13914-13924 pubmed publisher
    ..These data suggest specific functional and positional relationships among these components that explain genetic and molecular features of NHEJ and V(D)J recombination within cells. ..
  3. Grunebaum E, Bates A, Roifman C. Omenn syndrome is associated with mutations in DNA ligase IV. J Allergy Clin Immunol. 2008;122:1219-20 pubmed publisher