paneth cells

Summary

Summary: Differentiated epithelial cells of the INTESTINAL MUCOSA, found in the basal part of the intestinal crypts of Lieberkuhn. Paneth cells secrete GROWTH FACTORS, digestive enzymes such as LYSOZYME and antimicrobial peptides such as cryptdins (ALPHA-DEFENSINS) into the crypt lumen.

Top Publications

  1. Ireland H, Houghton C, Howard L, Winton D. Cellular inheritance of a Cre-activated reporter gene to determine Paneth cell longevity in the murine small intestine. Dev Dyn. 2005;233:1332-6 pubmed
    ..in their ability to express Cre from a somatically inducible transgene to determine the longevity of intestinal Paneth cells. In the Ahcre transgenic line induction of Cre recombinase allows constitutive activation of a Cre-activated ..
  2. Ogura Y, Lala S, Xin W, Smith E, Dowds T, Chen F, et al. Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis. Gut. 2003;52:1591-7 pubmed
    ..The reason for the unique association of NOD2 mutations with ileal disease is unclear. To identify a possible link, we tested expression of NOD2 in intestinal tissue of CD patients and controls...
  3. Tanigawa Y, Yakura R, Komiya T. The bHLH transcription factor Tcf12 (ME1) mRNA is abundantly expressed in Paneth cells of mouse intestine. Gene Expr Patterns. 2007;7:709-13 pubmed
    ..that mRNA coding for ME1, a basic helix-loop-helix (bHLH) transcription factor, was abundantly expressed in Paneth cells of adult small intestinal crypts...
  4. Elphick D, Mahida Y. Paneth cells: their role in innate immunity and inflammatory disease. Gut. 2005;54:1802-9 pubmed
  5. Formeister E, Sionas A, Lorance D, Barkley C, Lee G, Magness S. Distinct SOX9 levels differentially mark stem/progenitor populations and enteroendocrine cells of the small intestine epithelium. Am J Physiol Gastrointest Liver Physiol. 2009;296:G1108-18 pubmed publisher
    ..These data support a bimodal role for SOX9 in the intestinal epithelium, where low SOX9 expression supports proliferative capacity, and high SOX9 expression suppresses proliferation...
  6. Huynh D, Dai X, Nandi S, Lightowler S, Trivett M, Chan C, et al. Colony stimulating factor-1 dependence of paneth cell development in the mouse small intestine. Gastroenterology. 2009;137:136-44, 144.e1-3 pubmed publisher
    b>Paneth cells (PCs) secrete defensins and antimicrobial enzymes that contribute to innate immunity against pathogen infections within the mucosa of the small intestine...
  7. Suzuki K, Fukui H, Kayahara T, Sawada M, Seno H, Hiai H, et al. Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine. Biochem Biophys Res Commun. 2005;328:348-52 pubmed
    We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells...
  8. McElroy S, Underwood M, Sherman M. Paneth cells and necrotizing enterocolitis: a novel hypothesis for disease pathogenesis. Neonatology. 2013;103:10-20 pubmed publisher
    ..We suggest an alternate hypothesis wherein NEC is caused by injury to Paneth cells (PCs) in the intestinal crypts...
  9. Cunliffe R, Rose F, Keyte J, Abberley L, Chan W, Mahida Y. Human defensin 5 is stored in precursor form in normal Paneth cells and is expressed by some villous epithelial cells and by metaplastic Paneth cells in the colon in inflammatory bowel disease. Gut. 2001;48:176-85 pubmed
    ..Our aims were to (i) isolate, characterise, and investigate the processing of human defensin 5 (HD-5) in normal Paneth cells and (ii) investigate expression of HD-5 in active inflammatory bowel disease (IBD).

More Information

Publications62

  1. Wilson C, Ouellette A, Satchell D, Ayabe T, López Boado Y, Stratman J, et al. Regulation of intestinal alpha-defensin activation by the metalloproteinase matrilysin in innate host defense. Science. 1999;286:113-7 pubmed
    ..Thus, matrilysin functions in intestinal mucosal defense by regulating the activity of defensins, which may be a common role for this metalloproteinase in its numerous epithelial sites of expression...
  2. VanDussen K, Carulli A, Keeley T, Patel S, Puthoff B, Magness S, et al. Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells. Development. 2012;139:488-97 pubmed publisher
    ..Our findings suggest that Notch targets distinct progenitor cell populations to maintain adult intestinal stem cells and to regulate cell fate choice to control epithelial cell homeostasis. ..
  3. Plaisancie P, Claustre J, Estienne M, Henry G, Boutrou R, Paquet A, et al. A novel bioactive peptide from yoghurts modulates expression of the gel-forming MUC2 mucin as well as population of goblet cells and Paneth cells along the small intestine. J Nutr Biochem. 2013;24:213-21 pubmed publisher
    ..Oral administration of this peptide to rat pups enhanced the number of goblet cells and Paneth cells along the small intestine...
  4. Raetz M, Hwang S, Wilhelm C, Kirkland D, Benson A, Sturge C, et al. Parasite-induced TH1 cells and intestinal dysbiosis cooperate in IFN-?-dependent elimination of Paneth cells. Nat Immunol. 2013;14:136-42 pubmed publisher
    ..mucosal responses to the protozoan parasite Toxoplasma gondii resulted in dysbiosis and the elimination of Paneth cells. Paneth cell death led to loss of antimicrobial peptides and occurred in conjunction with uncontrolled ..
  5. Clevers H, Bevins C. Paneth cells: maestros of the small intestinal crypts. Annu Rev Physiol. 2013;75:289-311 pubmed publisher
    b>Paneth cells are highly specialized epithelial cells of the small intestine, where they coordinate many physiological functions...
  6. Andreu P, Peignon G, Slomianny C, Taketo M, Colnot S, Robine S, et al. A genetic study of the role of the Wnt/beta-catenin signalling in Paneth cell differentiation. Dev Biol. 2008;324:288-96 pubmed publisher
    ..We demonstrated that acute activation of Wnt/beta-catenin signalling induces de novo specification of Paneth cells in both the small intestine and colon and that colon cancers resulting from Apc mutations expressed many genes ..
  7. Shi J. Defensins and Paneth cells in inflammatory bowel disease. Inflamm Bowel Dis. 2007;13:1284-92 pubmed
    Defensins are antimicrobial peptides produced by professional phagocytes, Paneth cells, and intestinal epithelial cells...
  8. Durand A, Donahue B, Peignon G, Letourneur F, Cagnard N, Slomianny C, et al. Functional intestinal stem cells after Paneth cell ablation induced by the loss of transcription factor Math1 (Atoh1). Proc Natl Acad Sci U S A. 2012;109:8965-70 pubmed publisher
    ..an essential intrinsic signal for the maintenance and proliferation of CBC, and it was recently proposed that Paneth cells provide a crucial niche by secreting Wingless/Int (Wnt) ligands...
  9. Martinez Rodriguez N, Eloi M, Huynh A, Domínguez T, Lam A, Carcamo Molina D, et al. Expansion of Paneth cell population in response to enteric Salmonella enterica serovar Typhimurium infection. Infect Immun. 2012;80:266-75 pubmed publisher
    b>Paneth cells residing at the base of the small intestinal crypts contribute to the mucosal intestinal first line defense by secreting granules filled with antimicrobial polypeptides including lysozyme...
  10. Wang D, Peregrina K, Dhima E, Lin E, Mariadason J, Augenlicht L. Paneth cell marker expression in intestinal villi and colon crypts characterizes dietary induced risk for mouse sporadic intestinal cancer. Proc Natl Acad Sci U S A. 2011;108:10272-7 pubmed publisher
    ..This is fundamental for understanding how common sporadic tumors arise and in evaluating relative risk in the population...
  11. Ouellette A, Darmoul D, Tran D, Huttner K, Yuan J, Selsted M. Peptide localization and gene structure of cryptdin 4, a differentially expressed mouse paneth cell alpha-defensin. Infect Immun. 1999;67:6643-51 pubmed
    b>Paneth cells in crypts of the small intestine express antimicrobial peptides, including alpha-defensins, termed cryptdins in mice...
  12. McGuckin M, Linden S, Sutton P, Florin T. Mucin dynamics and enteric pathogens. Nat Rev Microbiol. 2011;9:265-78 pubmed publisher
    ..In addition, we describe dynamic alterations in the mucin barrier that are driven by host innate and adaptive immune responses to infection...
  13. Perminow G, Beisner J, Koslowski M, Lyckander L, Stange E, Vatn M, et al. Defective paneth cell-mediated host defense in pediatric ileal Crohn's disease. Am J Gastroenterol. 2010;105:452-9 pubmed publisher
    ..The aim of this study was to evaluate human alpha-defensin-5 (HD-5) and TCF-4 in an independent cohort of pediatric CD patients...
  14. Salzman N, Underwood M, Bevins C. Paneth cells, defensins, and the commensal microbiota: a hypothesis on intimate interplay at the intestinal mucosa. Semin Immunol. 2007;19:70-83 pubmed
    ..In the small intestine, Paneth cells (specialized secretory epithelial cells) produce high quantities of defensins and several other antibiotic ..
  15. Giblin L, Chang C, Bentley A, Frederickson C, Lippard S, Frederickson C. Zinc-secreting Paneth cells studied by ZP fluorescence. J Histochem Cytochem. 2006;54:311-6 pubmed
    ..family of zinc-specific-responsive fluorescent dyes (ZPs) to study the sequestration and secretion of zinc from Paneth cells, which are located in the bases of the crypts of Lieberkühn within the rat small intestine...
  16. Ayabe T, Satchell D, Wilson C, Parks W, Selsted M, Ouellette A. Secretion of microbicidal alpha-defensins by intestinal Paneth cells in response to bacteria. Nat Immunol. 2000;1:113-8 pubmed
    b>Paneth cells in mouse small intestinal crypts secrete granules rich in microbicidal peptides when exposed to bacteria or bacterial antigens...
  17. Bastide P, Darido C, Pannequin J, Kist R, Robine S, Marty Double C, et al. Sox9 regulates cell proliferation and is required for Paneth cell differentiation in the intestinal epithelium. J Cell Biol. 2007;178:635-48 pubmed
    ..factor Sox9 is expressed in the intestinal epithelium, specifically, in stem/progenitor cells and in Paneth cells. Sox9 expression requires an active beta-catenin-Tcf complex, the transcriptional effector of the Wnt pathway...
  18. Wang G, Stange E, Wehkamp J. Host-microbe interaction: mechanisms of defensin deficiency in Crohn's disease. Expert Rev Anti Infect Ther. 2007;5:1049-57 pubmed
    ..Knowledge regarding the regulation and biology of defensins provides an attractive target to open up new therapeutic avenues...
  19. Meineke F, Potten C, Loeffler M. Cell migration and organization in the intestinal crypt using a lattice-free model. Cell Prolif. 2001;34:253-66 pubmed
  20. FERNANDEZ M, Regnault B, Mulet C, Tanguy M, Jay P, Sansonetti P, et al. Maturation of paneth cells induces the refractory state of newborn mice to Shigella infection. J Immunol. 2008;180:4924-30 pubmed
    ..Indeed, expression of genes specific of Paneth cells was higher in 7-day- than in 4-day-old mice, and histological analysis indicated that Paneth cells were present ..
  21. Tanaka M, Saito H, Kusumi T, Fukuda S, Shimoyama T, Sasaki Y, et al. Spatial distribution and histogenesis of colorectal Paneth cell metaplasia in idiopathic inflammatory bowel disease. J Gastroenterol Hepatol. 2001;16:1353-9 pubmed
    ..Objectives of this research were to investigate the spatial distribution of PCM in IBD and other forms of colitis (non-IBD), and to find stimuli causing PCM...
  22. Gregorieff A, Stange D, Kujala P, Begthel H, van den Born M, Korving J, et al. The ets-domain transcription factor Spdef promotes maturation of goblet and paneth cells in the intestinal epithelium. Gastroenterology. 2009;137:1333-45.e1-3 pubmed publisher
    ..In this study, we investigated the role of the Wnt-responsive, Ets-domain transcription factor Spdef in the differentiation of goblet and Paneth cells.
  23. Kim T, Escudero S, Shivdasani R. Intact function of Lgr5 receptor-expressing intestinal stem cells in the absence of Paneth cells. Proc Natl Acad Sci U S A. 2012;109:3932-7 pubmed publisher
    ..Lgr5-expressing crypt base columnar cells (CBCs) reside deep in the crypt, mingled among mature Paneth cells that are well positioned for short-range signaling...
  24. Sato T, Vries R, Snippert H, van de Wetering M, Barker N, Stange D, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459:262-5 pubmed publisher
    ..We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche...
  25. Grootjans J, Hodin C, de Haan J, Derikx J, Rouschop K, Verheyen F, et al. Level of activation of the unfolded protein response correlates with Paneth cell apoptosis in human small intestine exposed to ischemia/reperfusion. Gastroenterology. 2011;140:529-539.e3 pubmed publisher
    In the intestine, Paneth cells participate in the innate immune response. Their highly secretory function makes them susceptible to environmental conditions that cause endoplasmic reticulum (ER) stress...
  26. Mastroianni J, Ouellette A. Alpha-defensins in enteric innate immunity: functional Paneth cell alpha-defensins in mouse colonic lumen. J Biol Chem. 2009;284:27848-56 pubmed publisher
    b>Paneth cells are a secretory epithelial lineage that release dense core granules rich in host defense peptides and proteins from the base of small intestinal crypts...
  27. Welz P, Wullaert A, Vlantis K, Kondylis V, Fernández Majada V, Ermolaeva M, et al. FADD prevents RIP3-mediated epithelial cell necrosis and chronic intestinal inflammation. Nature. 2011;477:330-4 pubmed publisher
    ..required for death-receptor-induced apoptosis, spontaneously developed epithelial cell necrosis, loss of Paneth cells, enteritis and severe erosive colitis...
  28. Gulati A, Shanahan M, Arthur J, Grossniklaus E, von Furstenberg R, Kreuk L, et al. Mouse background strain profoundly influences Paneth cell function and intestinal microbial composition. PLoS ONE. 2012;7:e32403 pubmed publisher
    Increasing evidence supports the central role of Paneth cells in maintaining intestinal host-microbial homeostasis. However, the direct impact of host genotype on Paneth cell function remains unclear...
  29. Batlle E, Henderson J, Beghtel H, van den Born M, Sancho E, Huls G, et al. Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB. Cell. 2002;111:251-63 pubmed
    ..Absorptive, enteroendocrine, and goblet cells migrate toward the villus while Paneth cells occupy the bottom of the crypts...
  30. Underwood M. Paneth cells and necrotizing enterocolitis. Gut Microbes. 2012;3:562-5 pubmed publisher
    ..Recent studies suggest a key role for Paneth cells in this disease...
  31. Stappenbeck T, Hooper L, Gordon J. Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells. Proc Natl Acad Sci U S A. 2002;99:15451-5 pubmed
    ..b>Paneth cells in the intestinal epithelium secrete antibacterial peptides that affect luminal microbial ecology...
  32. Salzman N, Ghosh D, Huttner K, Paterson Y, Bevins C. Protection against enteric salmonellosis in transgenic mice expressing a human intestinal defensin. Nature. 2003;422:522-6 pubmed
    ..b>Paneth cells, specialized epithelia of the small intestinal crypt, secrete abundant alpha-defensins and other antimicrobial ..
  33. Keshav S. Paneth cells: leukocyte-like mediators of innate immunity in the intestine. J Leukoc Biol. 2006;80:500-8 pubmed
    b>Paneth cells are secretory intestinal epithelial cells located at the base of the crypts of Lieberkühn in the small intestine...
  34. Simms L, Doecke J, Walsh M, Huang N, Fowler E, Radford Smith G. Reduced alpha-defensin expression is associated with inflammation and not NOD2 mutation status in ileal Crohn's disease. Gut. 2008;57:903-10 pubmed publisher
    ..The aim of this study was to independently assess whether NOD2, alpha-defensins and Crohn's disease are linked...
  35. Steenwinckel V, Louahed J, Lemaire M, Sommereyns C, Warnier G, McKenzie A, et al. IL-9 promotes IL-13-dependent paneth cell hyperplasia and up-regulation of innate immunity mediators in intestinal mucosa. J Immunol. 2009;182:4737-43 pubmed publisher
    ..Histochemical staining of Paneth cells by phloxine/tartrazine showed that IL-9 induces Paneth cell hyperplasia in Lieberkühn glands of the small ..
  36. Shanahan M, Tanabe H, Ouellette A. Strain-specific polymorphisms in Paneth cell ?-defensins of C57BL/6 mice and evidence of vestigial myeloid ?-defensin pseudogenes. Infect Immun. 2011;79:459-73 pubmed publisher
    b>Paneth cells at the base of small intestinal crypts secrete microbicidal ?-defensins, termed cryptdins (Crps) in mice, as mediators of innate immunity...
  37. Fukuzawa H, Sawada M, Kayahara T, Morita Fujisawa Y, Suzuki K, Seno H, et al. Identification of GM-CSF in Paneth cells using single-cell RT-PCR. Biochem Biophys Res Commun. 2003;312:897-902 pubmed
    b>Paneth cells, granule-containing cells located at the bottom of the intestinal crypts, have a role in innate mucosal immunity...
  38. Rumio C, Besusso D, Palazzo M, Selleri S, Sfondrini L, Dubini F, et al. Degranulation of paneth cells via toll-like receptor 9. Am J Pathol. 2004;165:373-81 pubmed
    The release of antimicrobial peptides and growth factors by Paneth cells is thought to play an important role in protecting the small intestine, but the mechanisms involved have remained obscure...
  39. Hodin C, Lenaerts K, Grootjans J, de Haan J, Hadfoune M, Verheyen F, et al. Starvation compromises Paneth cells. Am J Pathol. 2011;179:2885-93 pubmed publisher
    ..Recently, Paneth cells, important constituents of innate intestinal immunity, were found to be crucial in host protection against ..
  40. Hooper L, Stappenbeck T, Hong C, Gordon J. Angiogenins: a new class of microbicidal proteins involved in innate immunity. Nat Immunol. 2003;4:269-73 pubmed
    ..We show that a previously uncharacterized angiogenin, Ang4, is produced by mouse Paneth cells, is secreted into the gut lumen and has bactericidal activity against intestinal microbes...
  41. van Es J, Jay P, Gregorieff A, van Gijn M, Jonkheer S, Hatzis P, et al. Wnt signalling induces maturation of Paneth cells in intestinal crypts. Nat Cell Biol. 2005;7:381-6 pubmed
    ..Whereas all other differentiated epithelial cells migrate from the crypt onto the villus, Paneth cells home towards the source of Wnt signals--that is, the crypt bottom...
  42. Yang Q, Bermingham N, Finegold M, Zoghbi H. Requirement of Math1 for secretory cell lineage commitment in the mouse intestine. Science. 2001;294:2155-8 pubmed
    ..of four principal cell types deriving from one multipotent stem cell: enterocytes, goblet, enteroendocrine, and Paneth cells. Previous studies showed that Math1, a basic helix-loop-helix (bHLH) transcription factor, is expressed in the ..
  43. Bjerknes M, Cheng H. Cell Lineage metastability in Gfi1-deficient mouse intestinal epithelium. Dev Biol. 2010;345:49-63 pubmed publisher
  44. Strober W. Immunology. Unraveling gut inflammation. Science. 2006;313:1052-4 pubmed
  45. Di Sabatino A, Miceli E, Dhaliwal W, Biancheri P, Salerno R, Cantoro L, et al. Distribution, proliferation, and function of Paneth cells in uncomplicated and complicated adult celiac disease. Am J Clin Pathol. 2008;130:34-42 pubmed publisher
    b>Paneth cells, granulated epithelial cells located at the base of small bowel crypts, have a crucial role in innate immunity...
  46. Koslowski M, Kübler I, Chamaillard M, Schaeffeler E, Reinisch W, Wang G, et al. Genetic variants of Wnt transcription factor TCF-4 (TCF7L2) putative promoter region are associated with small intestinal Crohn's disease. PLoS ONE. 2009;4:e4496 pubmed publisher
    ..38, 95% CI 1.03 to1.84, p = 0.02882). The newly identified genetic association of TCF-4 with ileal CD provides evidence that the decrease in Paneth cell alpha-defensins is a primary factor in disease pathogenesis...
  47. El Shewy K, Eid R. In vivo killing of Giardia trophozoites harbouring bacterial endosymbionts by intestinal Paneth cells: an ultrastructural study. Parasitology. 2005;130:269-74 pubmed
    To date Paneth cells have not previously been reported to kill Giardia trophozoites and other protozoa in vivo. Here we report the first evidence for in vivo killing of Giardia trophozoites by intestinal Paneth cells...
  48. Zhang C, Sherman M, Prince L, Bader D, Weitkamp J, Slaughter J, et al. Paneth cell ablation in the presence of Klebsiella pneumoniae induces necrotizing enterocolitis (NEC)-like injury in the small intestine of immature mice. Dis Model Mech. 2012;5:522-32 pubmed publisher
    ..Normally, Paneth cells appear in the intestinal crypts during the first trimester of human pregnancy...
  49. Vaishnava S, Behrendt C, Ismail A, Eckmann L, Hooper L. Paneth cells directly sense gut commensals and maintain homeostasis at the intestinal host-microbial interface. Proc Natl Acad Sci U S A. 2008;105:20858-63 pubmed publisher
    ..To address this question we studied Paneth cells, a key small intestinal epithelial lineage...
  50. Okamoto R, Tsuchiya K, Nemoto Y, Akiyama J, Nakamura T, Kanai T, et al. Requirement of Notch activation during regeneration of the intestinal epithelia. Am J Physiol Gastrointest Liver Physiol. 2009;296:G23-35 pubmed publisher
    ..of Notch1 within intestinal epithelial cells, including PLA2G2A, an antimicrobial peptide secreted by Paneth cells. Finally, we show that these functions of activated Notch1 are present in the mucosa of ulcerative colitis, ..
  51. Thachil E, Hugot J, Arbeille B, Paris R, Grodet A, Peuchmaur M, et al. Abnormal activation of autophagy-induced crinophagy in Paneth cells from patients with Crohn's disease. Gastroenterology. 2012;142:1097-1099.e4 pubmed publisher
    ..We observed that autophagy was specifically activated in Paneth cells from patients with CD, independently of mucosal inflammation or disease-associated variants of ATG16L1 or IRGM...
  52. Wehkamp J, Stange E. Paneth's disease. J Crohns Colitis. 2010;4:523-31 pubmed publisher
    ..A characteristic feature of small intestinal host defense is the presence of Paneth cells at the bottom of the crypts of Lieberkühn...
  53. Li Q, Zhang Q, Wang C, Tang C, Zhang Y, Jiang S, et al. Influence of alemtuzumab on the intestinal Paneth cells and microflora in macaques. Clin Immunol. 2010;136:375-86 pubmed publisher
    ..We found the alterations of Paneth cells including abnormal Paneth cell granules and expression of lysozyme and defensin 5 in response to lymphocyte ..