bile canaliculi

Summary

Summary: Minute intercellular channels that occur between liver cells and carry bile towards interlobar bile ducts. Also called bile capillaries.

Top Publications

  1. McCaughan G, Wickson J, Creswick P, Gorrell M. Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence. Hepatology. 1990;11:534-44 pubmed
    ..It is concluded that these three molecules are almost certainly identical...
  2. Crocenzi F, Mottino A, Cao J, Veggi L, Pozzi E, Vore M, et al. Estradiol-17beta-D-glucuronide induces endocytic internalization of Bsep in rats. Am J Physiol Gastrointest Liver Physiol. 2003;285:G449-59 pubmed
    ..Mrp2 is required for E217G to induce its harmful effect...
  3. Mottino A, Crocenzi F, Pozzi E, Veggi L, Roma M, Vore M. Role of microtubules in estradiol-17beta-D-glucuronide-induced alteration of canalicular Mrp2 localization and activity. Am J Physiol Gastrointest Liver Physiol. 2005;288:G327-36 pubmed
    ..We conclude that recovery from E2-17G cholestasis, associated with exocytic insertion of Mrp2 in the canalicular membrane, but not its initial E2-17G-induced endocytosis, is a microtubule-dependent process...
  4. Oude Elferink R, Paulusma C. Function and pathophysiological importance of ABCB4 (MDR3 P-glycoprotein). Pflugers Arch. 2007;453:601-10 pubmed
    ..In this review, we will discuss the functional aspects of ABCB4 and the regulation of its expression. Furthermore, we will describe the clinical and biochemical consequences of complete and partial deficiency of ABCB4 function...
  5. Kikuchi S, Hata M, Fukumoto K, Yamane Y, Matsui T, Tamura A, et al. Radixin deficiency causes conjugated hyperbilirubinemia with loss of Mrp2 from bile canalicular membranes. Nat Genet. 2002;31:320-5 pubmed
    ..These findings indicate that radixin is required for secretion of conjugated bilirubin through its support of Mrp2 localization at BCMs...
  6. Mottino A, Cao J, Veggi L, Crocenzi F, Roma M, Vore M. Altered localization and activity of canalicular Mrp2 in estradiol-17beta-D-glucuronide-induced cholestasis. Hepatology. 2002;35:1409-19 pubmed
    ..In conclusion, these data indicate that E(2)17G induces endocytic internalization of Mrp2, which occurs in parallel with decreased bile flow and Mrp2 transport activity...
  7. Abu Absi S, Friend J, Hansen L, Hu W. Structural polarity and functional bile canaliculi in rat hepatocyte spheroids. Exp Cell Res. 2002;274:56-67 pubmed
    ..We hypothesized that polarity develops gradually and that the channels structurally and functionally resemble bile canaliculi. Immunofluorescence labeling of apical and basolateral proteins demonstrated reorganization of the membrane ..
  8. Rippin S, Hagenbuch B, Meier P, Stieger B. Cholestatic expression pattern of sinusoidal and canalicular organic anion transport systems in primary cultured rat hepatocytes. Hepatology. 2001;33:776-82 pubmed
  9. Becker A, Lucka L, Kilian C, Kannicht C, Reutter W. Characterisation of the ATP-dependent taurocholate-carrier protein (gp110) of the hepatocyte canalicular membrane. Eur J Biochem. 1993;214:539-48 pubmed
    ..In contrast, Fab fragments of these antibodies inhibit the aggregation of freshly isolated hepatocytes...

More Information

Publications62

  1. Remy L, Chalvet C, Ripert J, Gerolami A. Intracellular lumina and bile canaliculi in rat hepatocytes in vitro--a cytochemical study. Acta Histochem. 1989;85:87-92 pubmed
    ..These structures bear a strong resemblance to the bile canaliculi found in the liver...
  2. Wojtal K, Hoekstra D, Van IJzendoorn S. Anchoring of protein kinase A-regulatory subunit IIalpha to subapically positioned centrosomes mediates apical bile canalicular lumen development in response to oncostatin M but not cAMP. Mol Biol Cell. 2007;18:2745-54 pubmed
    ..The results also imply that multiple PKA-mediated signaling pathways control apical lumen development and that subapical centrosome positioning is important in some of these pathways...
  3. Marinelli R, Tietz P, Caride A, Huang B, Larusso N. Water transporting properties of hepatocyte basolateral and canalicular plasma membrane domains. J Biol Chem. 2003;278:43157-62 pubmed
    ..These data suggest a molecular mechanism for the efficient coupling of osmotically active solutes and water transport during canalicular bile formation...
  4. Van Der Wouden J, Van IJzendoorn S, Hoekstra D. Oncostatin M regulates membrane traffic and stimulates bile canalicular membrane biogenesis in HepG2 cells. EMBO J. 2002;21:6409-18 pubmed
    ..These data provide a clue for a molecular mechanism that couples the biogenesis of an apical plasma membrane domain to the regulation of intracellular transport in response to an extracellular, basolaterally localized stimulus...
  5. Byrne J, Meara N, Rayner A, Thompson R, Knisely A. Lack of hepatocellular CD10 along bile canaliculi is physiologic in early childhood and persistent in Alagille syndrome. Lab Invest. 2007;87:1138-48 pubmed
    ..Liver CD10 in childhood appears to undergo reduced synthesis or rapid degradation, which persists in AGS. Absence of CD10 expression thus may limit NEA as a marker of cholestasis in young patients and in AGS...
  6. Fu D, Wakabayashi Y, Ido Y, Lippincott Schwartz J, Arias I. Regulation of bile canalicular network formation and maintenance by AMP-activated protein kinase and LKB1. J Cell Sci. 2010;123:3294-302 pubmed publisher
    ..These studies reveal that AMPK and its upstream kinase, LKB1, regulate canalicular network formation and maintenance...
  7. Roma M, Milkiewicz P, Elias E, Coleman R. Control by signaling modulators of the sorting of canalicular transporters in rat hepatocyte couplets: role of the cytoskeleton. Hepatology. 2000;32:1342-56 pubmed
    ..A second pathway is activated by DBcAMP activation via Ca(2+)-calmodulin formation, whose requirements with respect to cytoskeletal components are opposite. PKC has a negative regulatory role in both pathways...
  8. Matsui H, Takeuchi S, Osada T, Fujii T, Sakai Y. Enhanced bile canaliculi formation enabling direct recovery of biliary metabolites of hepatocytes in 3D collagen gel microcavities. Lab Chip. 2012;12:1857-64 pubmed publisher
    ..present a hepatocyte culture configuration that enables the direct recovery of bile acid that accumulates in bile canaliculi by embedding the hepatocytes in a 3D micropatterned collagen gel substrate...
  9. Esteller A. Physiology of bile secretion. World J Gastroenterol. 2008;14:5641-9 pubmed
    ..The aim of this revision was to set the bases for other reviews in this series that will be devoted to specific issues related with biliary physiology and pathology...
  10. Calamita G, Mazzone A, Bizzoca A, Cavalier A, Cassano G, Thomas D, et al. Expression and immunolocalization of the aquaporin-8 water channel in rat gastrointestinal tract. Eur J Cell Biol. 2001;80:711-9 pubmed
    ..labeling was associated to hepatocyte intracellular vesicles and over the plasma membrane delimiting the bile canaliculi. A complex pattern was observed by immunoblotting with total membranes of the above GI organs incubated with ..
  11. Kipp H, Arias I. Trafficking of canalicular ABC transporters in hepatocytes. Annu Rev Physiol. 2002;64:595-608 pubmed
    ..e., bile secretory failure). Therefore, elucidation of the intrahepatic pathways and regulation of ABC transporters may help to understand the cause of cholestasis at a molecular level and provide clues for novel therapies...
  12. Takahashi R, Sonoda H, Tabata Y, Hisada A. Formation of hepatocyte spheroids with structural polarity and functional bile canaliculi using nanopillar sheets. Tissue Eng Part A. 2010;16:1983-95 pubmed publisher
    ..The spheroids obtained exhibited higher structural polarity and functional bile canaliculi compared with hepatocytes cultured using a conventional two-dimensional method.
  13. Gerloff T, Stieger B, Hagenbuch B, Madon J, Landmann L, Roth J, et al. The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver. J Biol Chem. 1998;273:10046-50 pubmed
    ..These results indicate that the sister of P-glycoprotein is the major canalicular bile salt export pump of mammalian liver...
  14. Rocken C, Licht J, Roessner A, Carl McGrath S. Canalicular immunostaining of aminopeptidase N (CD13) as a diagnostic marker for hepatocellular carcinoma. J Clin Pathol. 2005;58:1069-75 pubmed
  15. Shousha S, Gadir F, Peston D, Bansi D, Thillainaygam A, Murray Lyon I. CD10 immunostaining of bile canaliculi in liver biopsies: change of staining pattern with the development of cirrhosis. Histopathology. 2004;45:335-42 pubmed
    ..Our aim was to investigate the possibility that this loss of staining occurs prior to the development of cancer...
  16. Kipp H, Arias I. Newly synthesized canalicular ABC transporters are directly targeted from the Golgi to the hepatocyte apical domain in rat liver. J Biol Chem. 2000;275:15917-25 pubmed
    ..This study provides biochemical evidence for direct targeting of newly synthesized apical ABC transporters from the Golgi to the bile canaliculus in vivo...
  17. Wakabayashi Y, Dutt P, Lippincott Schwartz J, Arias I. Rab11a and myosin Vb are required for bile canalicular formation in WIF-B9 cells. Proc Natl Acad Sci U S A. 2005;102:15087-92 pubmed
    ..In WIF-B9 cells, which lack bile canaliculi, apical ABC transporters colocalized with transcytotic membrane proteins in rab11a-containing endosomes and, ..
  18. Ishigami A, Fujita T, Inoue H, Handa S, Kubo S, Kondo Y, et al. Senescence marker protein-30 (SMP30) induces formation of microvilli and bile canaliculi in Hep G2 cells. Cell Tissue Res. 2005;320:243-9 pubmed
    ..Subsequently, transmission electron microscopy revealed that groups of Hep G2/SMP30 cells exhibited bile canaliculi and possessed specialized adhesion contacts, such as tight junctions and desmosomes, at interplasmic ..
  19. Wakabayashi Y, Lippincott Schwartz J, Arias I. Intracellular trafficking of bile salt export pump (ABCB11) in polarized hepatic cells: constitutive cycling between the canalicular membrane and rab11-positive endosomes. Mol Biol Cell. 2004;15:3485-96 pubmed
    ..The pathway between canalicular and intracellular membranes that BSEP constitutively cycles within could serve to regulate apical pools of BSEP as well as other apical membrane transporters. ..
  20. Soroka C, Pate M, Boyer J. Canalicular export pumps traffic with polymeric immunoglobulin A receptor on the same microtubule-associated vesicle in rat liver. J Biol Chem. 1999;274:26416-24 pubmed
  21. Huebert R, Splinter P, Garcia F, Marinelli R, Larusso N. Expression and localization of aquaporin water channels in rat hepatocytes. Evidence for a role in canalicular bile secretion. J Biol Chem. 2002;277:22710-7 pubmed
    ..These results provide evidence that aquaporins are present in hepatocytes and that aquaporins are involved in agonist-stimulated canalicular bile secretion...
  22. Jansen P, Peters W, Meijer D. Hepatobiliary excretion of organic anions in double-mutant rats with a combination of defective canalicular transport and uridine 5'-diphosphate-glucuronyltransferase deficiency. Gastroenterology. 1987;93:1094-103 pubmed
  23. Joseph L, Yousef I, Plaa G, Sharkawi M. Potentiation of lithocholic-acid-induced cholestasis by methyl isobutyl ketone. Toxicol Lett. 1992;61:39-47 pubmed
    ..It is suggested that methyl isobutyl ketone potentiates lithocholate-induced cholestasis by reducing the bile salt pool and interfering with the haptic secretion rate of bile salts...
  24. Jessner W, Zsembery A, Graf J. Transcellular water transport in hepatobiliary secretion and role of aquaporins in liver. Wien Med Wochenschr. 2008;158:565-9 pubmed publisher
    ..The results are discussed with respect to the role of different types of membrane aquaporins being expressed in hepatocytes...
  25. Fouassier L, Kinnman N, Lefevre G, Lasnier E, Rey C, Poupon R, et al. Contribution of mrp2 in alterations of canalicular bile formation by the endothelin antagonist bosentan. J Hepatol. 2002;37:184-91 pubmed
    ..Bosentan, a dual endothelin ET(A/B) receptor antagonist, may cause dose-dependent reversible cholestatic liver injury. We herein tested whether bosentan or metabolites, both eliminated in bile, induce alterations in bile secretion...
  26. Wojtal K, de Vries E, Hoekstra D, Van IJzendoorn S. Efficient trafficking of MDR1/P-glycoprotein to apical canalicular plasma membranes in HepG2 cells requires PKA-RIIalpha anchoring and glucosylceramide. Mol Biol Cell. 2006;17:3638-50 pubmed
  27. Kidambi S, Patel S. Cholesterol and non-cholesterol sterol transporters: ABCG5, ABCG8 and NPC1L1: a review. Xenobiotica. 2008;38:1119-39 pubmed
    ..4. The objective of this review is to provide up-to-date knowledge on genomics, proteomics and function of these two transporter systems...
  28. Duguay A, Plaa G. Altered cholesterol synthesis as a mechanism involved in methyl isobutyl ketone-potentiated experimental cholestasis. Toxicol Appl Pharmacol. 1997;147:281-8 pubmed
    ..Therefore, increased BCM cholesterol content and enhanced accumulation of newly synthesized cholesterol appear to be involved in MiBK potentiation of Mn-BR-induced cholestasis...
  29. Wadsworth C, Dixon P, Wong J, Chapman M, McKay S, Sharif A, et al. Genetic factors in the pathogenesis of cholangiocarcinoma. Dig Dis. 2011;29:93-7 pubmed publisher
    ..In the current study, five biologically plausible candidate genes were investigated: ABCB11 (BSEP), ABCB4 (MDR3), ABCC2 (MRP2), ATP8B1 (FIC1) and NR1H4 (FXR)...
  30. West M, Hanigan M. ?-Glutamyl transpeptidase is a heavily N-glycosylated heterodimer in HepG2 cells. Arch Biochem Biophys. 2010;504:177-81 pubmed publisher
    ..and immunoaffinity analyses provide direct evidence that, in HepG2 cells, GGT is properly localized to the bile canaliculi. Three independent, experimental approaches demonstrate that GGT in HepG2 cells is comprised of two subunits ..
  31. Tomsik P, Sispera L, Rezacova M, Niang M, Stoklasova A, Cerman J, et al. Increased melibiose/rhamnose ratio in bile of rats with acute cholestasis. J Gastroenterol Hepatol. 2008;23:1934-40 pubmed publisher
  32. Li G, Pei Q, Gao Y, Liu K, Nie J, Han G, et al. Protective effects of hepatocellular canalicular conjugate export pump (Mrp2) on sodium arsenite-induced hepatic dysfunction in rats. Exp Toxicol Pathol. 2007;58:447-53 pubmed
    ..Electron microscopy showed that microvilli of bile canaliculi became swollen and sparse. ALT and AST activities in serum were markedly raised at 6 weeks...
  33. Wilasrusmee C, Siritheptawee S, Kanchanapanjapon S, Sopon P, Vanichanon C, Limpthong W, et al. Ultrastructural changes in cirrhotic and noncirrhotic patients due to hepatectomy. J Hepatobiliary Pancreat Surg. 2004;11:266-71 pubmed
    ..The aim of this investigation was to compare the ultrastructural changes in cirrhotic and noncirrhotic liver in response to ischemic and reperfusion injury due to hepatectomy...
  34. Zhang X, Sun J, Song M, Li F, Sun H. [Bile excretion of hepatocytes cultured in vitro]. Zhonghua Wai Ke Za Zhi. 2002;40:618-20 pubmed
    ..To regain bile excretory function of hepatocytes cultured in vitro...
  35. Meier Y, Pauli Magnus C, Zanger U, Klein K, Schaeffeler E, Nussler A, et al. Interindividual variability of canalicular ATP-binding-cassette (ABC)-transporter expression in human liver. Hepatology. 2006;44:62-74 pubmed
    ..Furthermore, data suggest a polymorphic transporter expression pattern, which might constitute a risk factor for the development of acquired forms of cholestatic liver diseases...
  36. Buchler M, Konig J, Brom M, Kartenbeck J, Spring H, Horie T, et al. cDNA cloning of the hepatocyte canalicular isoform of the multidrug resistance protein, cMrp, reveals a novel conjugate export pump deficient in hyperbilirubinemic mutant rats. J Biol Chem. 1996;271:15091-8 pubmed
    ..The results identify cMrp as a canalicular transport protein with a novel sequence and with a function similar to the one of the MRP...
  37. Sugimoto M, Inoko A, Shiromizu T, Nakayama M, Zou P, Yonemura S, et al. The keratin-binding protein Albatross regulates polarization of epithelial cells. J Cell Biol. 2008;183:19-28 pubmed publisher
    ..We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization...
  38. Wang R, Liu L, Sheps J, Forrest D, Hofmann A, Hagey L, et al. Defective canalicular transport and toxicity of dietary ursodeoxycholic acid in the abcb11-/- mouse: transport and gene expression studies. Am J Physiol Gastrointest Liver Physiol. 2013;305:G286-94 pubmed publisher
    ..UDCA fed to abcb11-/- mice caused liver damage and the appearance of biliary tetra- and penta-hydroxy bile acids. Supplementation with UDCA in the absence of Bsep caused adverse effects in abcb11-/- mice...
  39. Wang Y, Toh Y, Li Q, Nugraha B, Zheng B, Lu T, et al. Mechanical compaction directly modulates the dynamics of bile canaliculi formation. Integr Biol (Camb). 2013;5:390-401 pubmed publisher
  40. Akoume M, Perwaiz S, Yousef I, Plaa G. Synergistic role of 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol 7alpha-hydroxylase in the pathogenesis of manganese-bilirubin-induced cholestasis in rats. Toxicol Sci. 2003;73:378-85 pubmed
    ..In conclusion, the critical cholestatic events in Mn-Br-induced cholestasis appear to be mediated through the synergistic effects of Mn and BR acting on synthesis and degradation of cholesterol...
  41. Johnston H, Semmler Behnke M, Brown D, Kreyling W, Tran L, Stone V. Evaluating the uptake and intracellular fate of polystyrene nanoparticles by primary and hepatocyte cell lines in vitro. Toxicol Appl Pharmacol. 2010;242:66-78 pubmed publisher
    ..NPs accumulated within bile canaliculi to a limited extent, which suggests that NPs can be eliminated within bile...
  42. Stahl S, Davies M, Cook D, Graham M. Nuclear hormone receptor-dependent regulation of hepatic transporters and their role in the adaptive response in cholestasis. Xenobiotica. 2008;38:725-77 pubmed
    ..Furthermore, the complex networks between nuclear hormone receptors and regulated genes are illustrated and implications of targeting these receptors for the treatment of cholestasis are discussed...
  43. Cassio D, Macias R, Grosse B, Marin J, Monte M. Expression, localization, and inducibility by bile acids of hepatobiliary transporters in the new polarized rat hepatic cell lines, Can 3-1 and Can 10. Cell Tissue Res. 2007;330:447-60 pubmed
    ..CDCA and UDCA induced marked enhancement of the volume of Can 10 bile canaliculi. CDCA upregulated Bsep, Mdr2, SHP, Mdr1b, and Oatp2/1a4 in Can 10 (two- to seven-fold) and in Fao cells...
  44. Watanabe N, Kagawa T, Kojima S, Takashimizu S, Nagata N, Nishizaki Y, et al. Taurolithocholate impairs bile canalicular motility and canalicular bile secretion in isolated rat hepatocyte couplets. World J Gastroenterol. 2006;12:5320-5 pubmed
    ..To investigate the effects of taurolithocholate (TLC) on the canalicular motility in isolated rat hepatocyte couplets (IRHC)...
  45. Fukumoto K, Kikuchi S, Itoh N, Tamura A, Hata M, Yamagishi H, et al. Effects of genetic backgrounds on hyperbilirubinemia in radixin-deficient mice due to different expression levels of Mrp3. Biochim Biophys Acta. 2007;1772:298-306 pubmed
  46. Temme A, Stümpel F, Sohl G, Rieber E, Jungermann K, Willecke K, et al. Dilated bile canaliculi and attenuated decrease of nerve-dependent bile secretion in connexin32-deficient mouse liver. Pflugers Arch. 2001;442:961-6 pubmed
    ..To determine whether contraction of bile canaliculi and bile secretion are dependent on the function of gap junction channels, we compared wild-type and ..
  47. Harder C, Meng A, Rippstein P, McBride H, McPherson R. SR-BI undergoes cholesterol-stimulated transcytosis to the bile canaliculus in polarized WIF-B cells. J Biol Chem. 2007;282:1445-55 pubmed
    ..Taken together, these data demonstrate that hepatic SR-BI transcytosis is regulated by cholesterol and suggest that SR-BI has a stationary function on the bile canaliculus...
  48. Ismair M, Häusler S, Stuermer C, Guyot C, Meier P, Roth J, et al. ABC-transporters are localized in caveolin-1-positive and reggie-1-negative and reggie-2-negative microdomains of the canalicular membrane in rat hepatocytes. Hepatology. 2009;49:1673-82 pubmed publisher
    ..The canalicular transporters bile salt export pump, multidrug resistance protein 2, multidrug resistance-associated protein 2, and Abcg5 were largely resistant to Lubrol WX but were solubilized by Triton X-100...
  49. Takaki Y, Hirai S, Manabe N, Izumi Y, Hirose T, Nakaya M, et al. Dynamic changes in protein components of the tight junction during liver regeneration. Cell Tissue Res. 2001;305:399-409 pubmed
    ..junction is one of the most important events during liver regeneration leading to the reorganization of the bile canaliculi and the repolarization of hepatocytes after cell division...
  50. Benkoel L, Dodero F, Hardwigsen J, Campan P, Botta Fridlund D, Lombardo D, et al. Effect of ischemia-reperfusion on bile canalicular F-actin microfilaments in hepatocytes of human liver allograft: image analysis by confocal laser scanning microscopy. Dig Dis Sci. 2001;46:1663-7 pubmed
    ..This modification, probably induced by reactive oxygen species, could impair bile canalicular contraction and tight junction permeability and consequently bile secretion in the postoperative period...
  51. Song I, Lee Y, Chung S, Shim C. Multiple alterations of canalicular membrane transport activities in rats with CCl(4)-induced hepatic injury. Drug Metab Dispos. 2003;31:482-90 pubmed
    ..8- and 1.9-fold for daunomycin, unchanged for taurocholate, and decreased by 41 and 39% for E(2)17betaG, respectively, consistent with multiple alterations in the expression of the relevant transporters...
  52. Zanlungo S, Nervi F. Discovery of the hepatic canalicular and intestinal cholesterol transporters. New targets for treatment of hypercholesterolemia. Eur Rev Med Pharmacol Sci. 2003;7:33-9 pubmed
    ..The best theoretical drug should decrease serum lipoprotein cholesterol levels, increase biliary cholesterol secretion and fecal elimination and favoring at the same time gallbladder emptying to prevent gallstone formation...
  53. Ergun E, Ergun L, Essiz D. Light and electron microscopic studies on liver histology in chicks fed aflatoxin. Dtsch Tierarztl Wochenschr. 2006;113:363-8 pubmed
    ..Ultrastructural findings were destruction of rough endoplasmic reticulum (rER), reduction in mitochondrial size, enlargement of bile canaliculi, and cisternal dilatation of the smooth endoplasmic reticulum (sER).