two hybrid system techniques

Summary

Summary: Screening techniques used to identify genes encoding interacting proteins. Variations are used to evaluate complex interplay between proteins and other molecules.

Top Publications

  1. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
  2. pmc Evaluation of clustering algorithms for protein-protein interaction networks
    Sylvain Brohée
    Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
    BMC Bioinformatics 7:488. 2006
  3. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
  4. pmc High-quality binary protein interaction map of the yeast interactome network
    Haiyuan Yu
    Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 322:104-10. 2008
  5. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
  6. pmc Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis
    Gouzel Karimova
    Unité de Biochimie des Interactions Macromoléculaires, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Bacteriol 187:2233-43. 2005
  7. ncbi MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation
    A Trockenbacher
    Institute of Biochemistry, Peter Mayr Strasse 1a, 6020 Innsbruck, Austria
    Nat Genet 29:287-94. 2001
  8. ncbi TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis
    Heidi Szemenyei
    Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 319:1384-6. 2008
  9. ncbi A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans
    Sarah L Crittenden
    Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 417:660-3. 2002
  10. ncbi Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate
    Caryn Navarro
    Developmental Genetics Program and the Department of Cell Biology, The Skirball Institute and Howard Hughes Medical Institute, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Nat Cell Biol 6:427-35. 2004

Detail Information

Publications311 found, 100 shown here

  1. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
    ..We propose that the small G-protein kir/Gem, interacting with beta-subunits, regulates Ca2+ channel expression at the cell surface...
  2. pmc Evaluation of clustering algorithms for protein-protein interaction networks
    Sylvain Brohée
    Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
    BMC Bioinformatics 7:488. 2006
    ..In this paper we present a comparative assessment of four algorithms: Markov Clustering (MCL), Restricted Neighborhood Search Clustering (RNSC), Super Paramagnetic Clustering (SPC), and Molecular Complex Detection (MCODE)...
  3. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
    ..This effect decreases the likelihood of cross talk between different functional modules of the cell and increases the overall robustness of a network by localizing effects of deleterious perturbations...
  4. pmc High-quality binary protein interaction map of the yeast interactome network
    Haiyuan Yu
    Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 322:104-10. 2008
    ..Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy...
  5. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies...
  6. pmc Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis
    Gouzel Karimova
    Unité de Biochimie des Interactions Macromoléculaires, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Bacteriol 187:2233-43. 2005
    ..In addition, our study shows that the cAMP-based two-hybrid system is particularly appropriate for analyzing molecular interactions between membrane proteins...
  7. ncbi MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation
    A Trockenbacher
    Institute of Biochemistry, Peter Mayr Strasse 1a, 6020 Innsbruck, Austria
    Nat Genet 29:287-94. 2001
    ..Elevated PP2Ac causes hypophosphorylation of MAPs, a pathological mechanism that is consistent with the OS phenotype...
  8. ncbi TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis
    Heidi Szemenyei
    Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 319:1384-6. 2008
    ..These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development...
  9. ncbi A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans
    Sarah L Crittenden
    Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 417:660-3. 2002
    ..We suggest that regulation by PUF proteins may be an ancient and widespread mechanism for control of stem cells...
  10. ncbi Egalitarian binds dynein light chain to establish oocyte polarity and maintain oocyte fate
    Caryn Navarro
    Developmental Genetics Program and the Department of Cell Biology, The Skirball Institute and Howard Hughes Medical Institute, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Nat Cell Biol 6:427-35. 2004
    ..Our data provide a direct link between a molecule necessary for oocyte specification and the microtubule motor complex, and supports the hypothesis that microtubule-mediated transport is important for preserving oocyte fate...
  11. pmc Nervous wreck interacts with thickveins and the endocytic machinery to attenuate retrograde BMP signaling during synaptic growth
    Kate M O'Connor-Giles
    Laboratory of Genetics, University of Wisconsin Madison, Madison, WI 53706, USA
    Neuron 58:507-18. 2008
    ..We propose that Nwk functions at the interface of endocytosis and BMP signaling to ensure proper synaptic growth by negatively regulating Tkv to set limits on this positive growth signal...
  12. pmc Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins
    Juan Martin-Serrano
    Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:12414-9. 2003
    ....
  13. ncbi Sensing of Lys 63-linked polyubiquitination by NEMO is a key event in NF-kappaB activation [corrected]
    Chuan Jin Wu
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 8:398-406. 2006
    ..These results provide a mechanism for NEMO's critical role in IKK activation, and a key to understanding the link between cytokine-receptor proximal signalling and IKK and NF-kappaB activation...
  14. pmc Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK
    Chie Kanei-Ishii
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305 0074, Japan
    Genes Dev 18:816-29. 2004
    ..The down-regulation of Myb by Wnt-1 signal may play an important role in a variety of developmental steps...
  15. ncbi PHD domain-mediated E3 ligase activity directs intramolecular sumoylation of an adjacent bromodomain required for gene silencing
    Alexey V Ivanov
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell 28:823-37. 2007
    ..These data provide a mechanistic explanation for the cooperation of PHD and bromodomains in gene regulation and describe a function of the PHD domain as an intramolecular E3 SUMO ligase...
  16. pmc Group 13 HOX proteins interact with the MH2 domain of R-Smads and modulate Smad transcriptional activation functions independent of HOX DNA-binding capability
    Thomas M Williams
    Department of Human Genetics, University of Michigan Ann Arbor, MI, USA
    Nucleic Acids Res 33:4475-84. 2005
    ....
  17. ncbi The Caenorhabditis elegans Skp1-related gene family: diverse functions in cell proliferation, morphogenesis, and meiosis
    Sudhir Nayak
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Biol 12:277-87. 2002
    ..In contrast to yeast and humans, Caenorhabditis elegans contains multiple expressed Skp1-related (skr) genes...
  18. ncbi BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3
    Lai Xu
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nature 425:316-21. 2003
    ..elegans CUL-3. Biochemical studies using the BTB protein MEL-26 and its genetic target MEI-1 (refs 12, 13) indicate that BTB proteins merge the functional properties of Skp1 and F-box proteins into a single polypeptide...
  19. ncbi Multiple Skp1-related proteins in Caenorhabditis elegans: diverse patterns of interaction with Cullins and F-box proteins
    Atsushi Yamanaka
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, 812 8582, Fukuoka, Japan
    Curr Biol 12:267-75. 2002
    ..The biochemical properties, expression, and function of the C. elegans SKR proteins were examined...
  20. pmc NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway
    M Frank-Vaillant
    Laboratoire du Cycle Cellulaire, Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Genes Dev 15:3005-12. 2001
    ..Increasing the dosage of the Lif2p protein can suppress the NHEJ defect in a/alpha cells. Together, these results indicate that NHEJ regulation by mating type is achieved, at least in part, by a regulation of Lif2p activity...
  21. pmc Drosophila Brain Tumor is a translational repressor
    J Sonoda
    Howard Hughes Medical Institute, Department of Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genes Dev 15:762-73. 2001
    ..The results of these experiments suggest that NHL domain proteins are recruited to various mRNAs by combinatorial protein-protein interactions...
  22. ncbi A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor
    M Fedele
    Centro di Endocrinologia ed Oncologia Sperimentale del CNR G Salvatore Dipartimento di Biologia e Patologia Cellulare e Molecolare L Califano Università degli Studi di Napoli Federico II via S Pansini, 5, 80131 Napoli, Italy
    J Biol Chem 275:7894-901. 2000
    ..In addition, RNF4 was also found to associate with HMGI(Y), a chromatin-modeling factor containing AT-hook domains...
  23. pmc Protein interaction mapping: a Drosophila case study
    Etienne Formstecher
    Hybrigenics, 75014 Paris, France
    Genome Res 15:376-84. 2005
    ..Analysis of the differences in data sets and methods suggests alternative strategies to enhance the accuracy and comprehensiveness of the post-genomic generation of broad-scale protein interaction maps...
  24. ncbi A pathway for association of receptors, adaptors, and actin during endocytic internalization
    Marko Kaksonen
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 115:475-87. 2003
    ..Attention is now focused on the mechanisms by which the order and timing of events in this endocytic pathway are achieved...
  25. ncbi Domain II mutations in CRANE/IAA18 suppress lateral root formation and affect shoot development in Arabidopsis thaliana
    Takeo Uehara
    Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai, Kobe, 657 8501 Japan
    Plant Cell Physiol 49:1025-38. 2008
    ..Taken together, our results indicate that CRANE/IAA18 is involved in lateral root formation in Arabidopsis, and suggest that it negatively regulates the activity of ARF7 and ARF19 for lateral root formation...
  26. ncbi C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes
    A Craig Mackinnon
    Department of Cell and Structural Biology, University of Illinois, Urbana Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    Curr Biol 12:787-97. 2002
    ..ILK has been implicated in integrin-mediated signaling and is also an adaptor within integrin-associated cytoskeletal complexes...
  27. pmc Comprehensive interaction map of the Arabidopsis MADS Box transcription factors
    Stefan de Folter
    Business Unit Bioscience, Plant Research International, 6700 AA Wageningen, The Netherlands
    Plant Cell 17:1424-33. 2005
    ..Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower...
  28. pmc ZNF265--a novel spliceosomal protein able to induce alternative splicing
    D J Adams
    The University of Sydney, Basic and Clinical Genomics Laboratory, Department of Physiology and Institute for Biomedical Research, Sydney, NSW 2006, Australia
    J Cell Biol 154:25-32. 2001
    ..Alignment with other RS domain-containing proteins revealed a high degree of SR dipeptide conservation. These data show that ZNF265 functions as a novel component of the mRNA processing machinery...
  29. ncbi The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif
    K W Lo
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People s Republic of China
    J Biol Chem 276:14059-66. 2001
    ..The role of each conserved amino acid residue in this pentapeptide motif in supporting complex formation with DLC8 was systematically studied using site-directed mutagenesis...
  30. ncbi Galpha13 stimulates cell migration through cortactin-interacting protein Hax-1
    V Radhika
    Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 279:49406-13. 2004
    ..These findings, along with the observation that Hax-1 is overexpressed in metastatic tumors and tumor cell lines, suggest a novel role for the association of oncogenic Galpha13 and Hax-1 in tumor metastasis...
  31. ncbi Interaction of c-Jun amino-terminal kinase interacting protein-1 with p190 rhoGEF and its localization in differentiated neurons
    D Meyer
    Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 274:35113-8. 1999
    ..The interaction of JIP-1 with rhoGEF and its subcellular localization suggests that JIP-1 may function to specifically localize a signaling complex in neuronal cells...
  32. ncbi HOXA5-twist interaction alters p53 homeostasis in breast cancer cells
    Ioannis A Stasinopoulos
    Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:2294-9. 2005
    ..Thus, the loss of HOXA5 expression could lead to the functional activation of Twist resulting in aberrant cell cycle regulation and promoting breast carcinogenesis...
  33. ncbi Matrix and Steiner-triple-system smart pooling assays for high-performance transcription regulatory network mapping
    Vanessa Vermeirssen
    Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Methods 4:659-64. 2007
    ..Both strategies outperform library screens in terms of coverage, confidence and throughput. These versatile strategies can be adapted both to TFs in other systems and, likely, to other biomolecules and assays as well...
  34. ncbi Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74
    Christian Schwarzer
    Max Planck Institute for Experimental Medicine, Department of Immunochemistry, Hermann Rein Street 3, 37075 Gottingen, Germany
    Int J Biochem Cell Biol 34:1059-70. 2002
    ....
  35. pmc The tripartite motif family identifies cell compartments
    A Reymond
    Telethon Institute of Genetics and Medicine TIGEM, San Raffaele Biomedical Science Park, Milan, Italy
    EMBO J 20:2140-51. 2001
    ..A total of 37 TRIM genes/proteins were studied, 21 of which were novel. The results demonstrate that TRIM proteins share a common function: by means of homo-multimerization they identify specific cell compartments...
  36. ncbi Snx5, as a Mind bomb-binding protein, is expressed in hematopoietic and endothelial precursor cells in zebrafish
    Kyeong Won Yoo
    Department of Biology, Chungnam National University, Daejeon 305 764, South Korea
    FEBS Lett 580:4409-16. 2006
    ..Taken together, we show that Snx5, a novel interacting partner of Mind bomb, may have an essential role for cell fate determination in early development...
  37. pmc A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene
    Kwang Hoon Song
    Department of Microbiology, Immunology and Biochemistry, Northeastern Ohio University College of Medicine, 4209 State Route 44, Rootstown, OH 44272, USA
    J Biol Chem 281:10081-8. 2006
    ..These results suggest that Prox1 is a novel co-regulator of HNF4alpha that may play a key role in the regulation of bile acid synthesis and gluconeogenesis in the liver...
  38. ncbi Specific interaction of the potassium channel beta-subunit minK with the sarcomeric protein T-cap suggests a T-tubule-myofibril linking system
    T Furukawa
    Department of Physiology, Akita University School of Medicine, Japan
    J Mol Biol 313:775-84. 2001
    ..We speculate that this interaction may contribute to a stretch-dependent regulation of potassium flux in cardiac muscle, providing a "mechano-electrical feedback" system...
  39. ncbi Yeast Sm-like proteins function in mRNA decapping and decay
    S Tharun
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson 85721, USA
    Nature 404:515-8. 2000
    ..In addition, the Lsm complex that functions in mRNA decay appears to be distinct from the U6-associated Lsm complex, indicating that Lsm proteins form specific complexes that affect different aspects of mRNA metabolism...
  40. ncbi Ligand-induced ubiquitination of the epidermal growth factor receptor involves the interaction of the c-Cbl RING finger and UbcH7
    M Yokouchi
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 274:31707-12. 1999
    ..This mechanism participates in the down-regulation of tyrosine kinase receptors and loss of this function, as occurs in the naturally occurring 70Z-Cbl isoform, probably contributes to oncogenic transformation...
  41. pmc HIV-1 Vpr-mediated G2 arrest involves the DDB1-CUL4AVPRBP E3 ubiquitin ligase
    Jean Philippe Belzile
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada
    PLoS Pathog 3:e85. 2007
    ....
  42. ncbi Linking beta-catenin to androgen-signaling pathway
    Fajun Yang
    Department of Surgery and Department of Genetics, Stanford University School of Medicine, Stanford, California 94305 5328, USA
    J Biol Chem 277:11336-44. 2002
    ....
  43. ncbi Characterization of a carboxy-terminal BRCA1 interacting protein
    A K Wong
    Myriad Genetics, Inc, Salt Lake City, Utah 84108, USA
    Oncogene 17:2279-85. 1998
    ..Thus it is plausible that CtIP may itself be a tumor suppressor acting in the same pathway as BRCA1...
  44. ncbi Comparative assessment of large-scale data sets of protein-protein interactions
    Christian von Mering
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany
    Nature 417:399-403. 2002
    ..To measure their accuracy and potential as well as to identify biases, strengths and weaknesses, we compare the methods with each other and with a reference set of previously reported protein interactions...
  45. pmc A dual mechanism controlling the localization and function of exocytic v-SNAREs
    Sonia Martinez-Arca
    Membrane Traffic and Neuronal Plasticity, Institut National de la Santé et de la Recherche Médicale U536, Institut du Fer a Moulin, 75005 Paris, France
    Proc Natl Acad Sci U S A 100:9011-6. 2003
    ..Moreover, they point to the amino-terminal domains of SNARE proteins as multifunctional modules responsible for the fine tuning of SNARE function...
  46. ncbi Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae
    J Thomas Hannich
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:4102-10. 2005
    ..The results point to a surprisingly broad array of cellular processes regulated by SUMO conjugation and provide a starting point for detailed studies of how SUMO ligation contributes to these different regulatory mechanisms...
  47. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  48. ncbi Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB
    Simona Masiero
    Dipartimento di Genetica e Biologia dei Microrganismi, Universita di Milano, Via Celoria 26, 20133 Milano, Italy
    J Biol Chem 277:26429-35. 2002
    ..This is the first indication that members of the NF-YB family work through mechanisms independent of the CCAAT box...
  49. pmc The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity
    J E Adamo
    Department of Cell Biology, Cell Biology, and Genetics, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Biol Cell 10:4121-33. 1999
    ..These data suggest that Rho3 acts as a key regulator of cell polarity and exocytosis, coordinating several distinct events for delivery of proteins to specific sites on the cell surface...
  50. ncbi Annexin II light chain regulates sensory neuron-specific sodium channel expression
    Kenji Okuse
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Nature 417:653-6. 2002
    ..Because direct association with p11 is required for functional expression of Na(V)1.8, disrupting this interaction may be a useful new approach to downregulating Na(V)1.8 and effecting analgesia...
  51. ncbi Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2
    Stephan Lange
    Institute of Cell Biology, ETH Honggerberg, 8093 Zurich, Switzerland
    J Cell Sci 115:4925-36. 2002
    ..We propose that DRAL/FHL-2 acts as a specific adaptor protein to couple metabolic enzymes to sites of high energy consumption in the cardiac sarcomere...
  52. pmc Human Vam6p promotes lysosome clustering and fusion in vivo
    S Caplan
    Cell Biology and Metabolism Branch at the National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 154:109-22. 2001
    ..This study implicates hVam6p as a mammalian tethering/docking factor characterized with intrinsic ability to promote lysosome fusion in vivo...
  53. ncbi Negative regulation of the mammalian UV response by Myc through association with Miz-1
    Steffi Herold
    Institute for Molecular Biology and Tumor Research, 35033 Marburg, Germany
    Mol Cell 10:509-21. 2002
    ..Our data implicate Miz-1 in a pathway that regulates cell proliferation in response to UV irradiation...
  54. ncbi A protein interaction network of the malaria parasite Plasmodium falciparum
    Douglas J LaCount
    Howard Hughes Medical Institute, University of Washington, Box 357730, Seattle, Washington 98195, USA
    Nature 438:103-7. 2005
    ..These data constitute the first extensive description of the protein interaction network for this important human pathogen...
  55. pmc Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex
    Leila Alland
    Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 22:2743-50. 2002
    ....
  56. pmc The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex
    Beatriz Aranda-Orgilles
    Max Planck Institute for Molecular Genetics, Ihnestr 73, 14195, Berlin, Germany
    Hum Genet 123:163-76. 2008
    ..The dysfunction of this mechanism would lead to malfunction of microtubule-associated protein translation and to the development of OS...
  57. ncbi Mammalian 14-3-3beta associates with the Chlamydia trachomatis inclusion membrane via its interaction with IncG
    M A Scidmore
    Host Parasite Interactions Section, Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Mol Microbiol 39:1638-50. 2001
    ..psittaci or C. pneumoniae inclusions. 14-3-3beta is the first eukaryotic protein found to interact with the chlamydial inclusion; however, its unique role in C. trachomatis pathogenesis remains to be determined...
  58. pmc Abscisic acid signaling in seeds and seedlings
    Ruth R Finkelstein
    Department of Molecular, Cellular, and Developmental Biology, University of California at Santa Barbara, Santa Barbara, CA 93106, USA
    Plant Cell 14:S15-45. 2002
  59. ncbi Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain
    T Centner
    European Molecular Biology Laboratory, Heidelberg, D 69117, Germany
    J Mol Biol 306:717-26. 2001
    ....
  60. ncbi A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease
    Heike Goehler
    Max Delbrueck Center for Molecular Medicine, 13125 Berlin Buch, Germany
    Mol Cell 15:853-65. 2004
    ..Moreover, GIT1 localizes to neuronal inclusions, and is selectively cleaved in HD brains, indicating that its distribution and function is altered during disease pathogenesis...
  61. pmc Interactions with Costal2 and suppressor of fused regulate nuclear translocation and activity of cubitus interruptus
    G Wang
    Center for Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9133, USA
    Genes Dev 14:2893-905. 2000
    ..Finally, we show that Cos2 is required for transducing high levels of Hh signaling activity, and it does so by alleviating the blockage of Ci activity imposed by Su(fu)...
  62. ncbi WRN interacts physically and functionally with the recombination mediator protein RAD52
    Kathy Baynton
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, Rikshospitalet, 0027 Oslo, Norway
    J Biol Chem 278:36476-86. 2003
    ..These results suggest that coordinated WRN and RAD52 activities are involved in replication fork rescue after DNA damage...
  63. pmc Pim serine/threonine kinases regulate the stability of Socs-1 protein
    X Peter Chen
    Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 99:2175-80. 2002
    ..These data suggest that the Pim kinases may regulate cytokine-induced JAK-STAT signaling through modulation of Socs-1 protein levels...
  64. ncbi SoxE factors function equivalently during neural crest and inner ear development and their activity is regulated by SUMOylation
    Kimberly M Taylor
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Dev Cell 9:593-603. 2005
    ..These findings provide important mechanistic insight into how the activity of widely deployed developmental regulatory proteins can be directed to specific developmental events...
  65. pmc Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases
    Hitoshi Yoshida
    Department of Rice Research, National Agricultural Research Center, Jo etsu, Niigata 943 0193, Japan
    BMC Plant Biol 5:14. 2005
    ..Because orthologous sequences to ETO1 are found in many plant species including tomato, we transformed tomato with Arabidopsis ETO1 to evaluate its ability to suppress ethylene production in tomato fruits...
  66. pmc Notch-induced E2A degradation requires CHIP and Hsc70 as novel facilitators of ubiquitination
    Zhong Huang
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, 825 NE 13th St, Oklahoma City, OK 73104, USA
    Mol Cell Biol 24:8951-62. 2004
    ..CHIP also associates with Cul1, which introduces PUC to the SCF E3 ligase complex, responsible for E47 ubiquitination. Therefore, CHIP plays a crucial role in the ubiquitination and degradation of E2A proteins...
  67. ncbi Post-translational modification of Rta of Epstein-Barr virus by SUMO-1
    Li Kwan Chang
    Faculty of Biological Medicine and Environmental Biology and Graduate Institute of Biochemistry, Kaohsiung Medical University, 100, Shih Chuan 1st Road, Kaohsiung, Taiwan
    J Biol Chem 279:38803-12. 2004
    ..These results indicate that sumoylation of Rta may be important in EBV lytic activation...
  68. pmc MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana
    Kiyoshi Tatematsu
    Division of Biological Sciences, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Plant Cell 16:379-93. 2004
    ..These results suggest that MSG2/IAA19 and NPH4/ARF7 may constitute a negative feedback loop to regulate differential growth responses of hypocotyls and lateral root formation...
  69. pmc The formin-homology protein SmDia interacts with the Src kinase SmTK and the GTPase SmRho1 in the gonads of Schistosoma mansoni
    Thomas Quack
    Institute for Parasitology, Justus Liebig University, Giessen, Germany
    PLoS ONE 4:e6998. 2009
    ..Since Src kinases are important signal transduction proteins it is of interest to unravel the signaling cascades SmTK3 is involved in to understand its cellular role in the gonads...
  70. ncbi Complexes of MADS-box proteins are sufficient to convert leaves into floral organs
    T Honma
    Institute for Chemical Research, Kyoto University, Uji, Japan
    Nature 409:525-9. 2001
    ....
  71. pmc CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription
    Linyu Li
    Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology and School of Medicine, Tsinghua University, Beijing 100084, People s Republic of China
    Mol Cell Biol 24:856-64. 2004
    ..These results suggest that CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins...
  72. ncbi Analyzing mRNA-protein complexes using a yeast three-hybrid system
    David S Bernstein
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Methods 26:123-41. 2002
    ..Multicomponent complexes containing more than one protein can be detected, identified, and analyzed. We describe the method and how to use it, and discuss applications that bear particularly on eukaryotic mRNAs...
  73. ncbi Analysis of multimerization of the SARS coronavirus nucleocapsid protein
    Runtao He
    National Microbiology Laboratory, Health Canada, 1015 Arlington St, Winnipeg, MB, Canada R3E 3R2
    Biochem Biophys Res Commun 316:476-83. 2004
    ..Collectively, these results may aid us in elucidating the mechanism pertaining to formation of viral nucleocapsid core, and designing molecular approaches to intervene SARS-CoV replication...
  74. pmc Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules
    Takeshi Nakamura
    Department of Molecular Genetics, National Institute for Longevity Sciences, Program of Protecting the Brain, CREST, JST, Oobu, Aichi 474 8522, Japan
    Mol Cell Biol 22:8721-34. 2002
    ..These results suggest that Grit, a novel TrkA-interacting protein, regulates neurite outgrowth by modulating the Rho family of small GTPases...
  75. pmc Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins
    Katsumi Kasashima
    Department of Biology, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    Nucleic Acids Res 30:4519-26. 2002
    ..These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs...
  76. pmc Enhanceosome formation over the beta interferon promoter underlies a remote-control mechanism mediated by YY1 and YY2
    Martin Klar
    German Research Center for Biotechnology GBF, RDIF Epigenetic Regulation, Mascheroder Weg 1, 38124 Braunschweig, Germany
    Mol Cell Biol 25:10159-70. 2005
    ..Differences between the human and murine control regions will be described...
  77. ncbi A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae
    S L Ooi
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Science 294:2552-6. 2001
    ..Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer...
  78. ncbi E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages
    Devin E Christensen
    Department of Biochemistry, University of Washington, Box 357350, Seattle, Washington 98195 7350, USA
    Nat Struct Mol Biol 14:941-8. 2007
    ..Thus, BRCA1 has the ability to direct the synthesis of specific polyubiquitin chain linkages, depending on the E2 bound to its RING...
  79. ncbi TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation
    R Perlman
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 3:708-14. 2001
    ..Our findings associate Daxx directly with the TGF-beta apoptotic-signalling pathway, and make a biochemical connection between the receptors for TGF-beta and the apoptotic machinery...
  80. ncbi Myosin V orientates the mitotic spindle in yeast
    H Yin
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nature 406:1013-5. 2000
    ..The tail domain of Myo2 that binds Kar9 also interacts with secretory vesicles and vacuolar elements, making it a pivotal component of yeast cell polarization...
  81. ncbi TrioGEF1 controls Rac- and Cdc42-dependent cell structures through the direct activation of rhoG
    A Blangy
    Centre de Recherche en Biochimie Macromoleculaire, CRBM CNRS UPR1086, IFR24, 34293 Montpellier Cedex 5, France
    J Cell Sci 113:729-39. 2000
    ..These data demonstrate that Trio controls a signaling cascade that activates RhoG, which in turn activates Rac1 and Cdc42Hs...
  82. pmc The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases
    V Albrecht
    Universitat Ulm, Molekulare Botanik, Albert Einstein Allee 11, D 89069 Ulm, Germany
    EMBO J 20:1051-63. 2001
    ....
  83. ncbi FANCC, FANCE, and FANCD2 form a ternary complex essential to the integrity of the Fanconi anemia DNA damage response pathway
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:36118-25. 2005
    ..Thus, FANCE is shown to be a key mediator of protein interactions both in the architecture of the FA protein complex and in the connection of complex components to the putative downstream targets of complex activity...
  84. ncbi Gib2, a novel Gbeta-like/RACK1 homolog, functions as a Gbeta subunit in cAMP signaling and is essential in Cryptococcus neoformans
    Daniel A Palmer
    Research Institute for Children, Department of Pediatrics, Louisiana State University Health Sciences Center, 200 Henry Clay Avenue, New Orleans, LA 70118, USA
    J Biol Chem 281:32596-605. 2006
    ..Finally, Gib2 is shown to also physically interact with a downstream target of Gpa1-cAMP signaling, Smg1, and the protein kinase C homolog Pkc1, indicating that Gib2 is also a multifunctional RACK1-like protein...
  85. ncbi The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC
    France Léveillé
    Department of Clinical Genetics and Human Genetics, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    DNA Repair (Amst) 5:556-65. 2006
    ..This strengthens the idea that FANCE recruits FANCD2 to the core complex, without interfering with the binding of FANCC...
  86. pmc Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome
    Joy Armistead
    Department of Biochemistry and Medical Genetics, University of Manitoba, MB, Canada
    Am J Hum Genet 84:728-39. 2009
    ..These findings suggested that the D-to-G mutation caused aggregation of EMG1, thereby reducing the level of the protein and causing BCS...
  87. ncbi Myotilin is mutated in limb girdle muscular dystrophy 1A
    M A Hauser
    Department of Medicine, Section of Medical Genetics, Duke University Medical Center, DUMC 3445, Durham, NC 27710 3445, USA
    Hum Mol Genet 9:2141-7. 2000
    ..Myotilin is a sarcomeric protein that binds to alpha-actinin and is localized in the Z-line. The observed missense mutation does not disrupt binding to alpha-actinin...
  88. pmc Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein
    K Yeung
    Department of Molecular Biology, Brown University, Providence, Rhode Island 02912, USA
    Mol Cell Biol 20:3079-85. 2000
    ..The properties of RKIP reveal the specific sequestration of interacting components as a novel motif in the cell's repertoire for the regulation of signaling pathways...
  89. ncbi The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein
    Isabelle Imbert
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, Marseille Cedex 9, France
    Virus Res 133:136-48. 2008
    ..The interaction map thus provides a sum of the interactions that may occur at some point during coronavirus RNA synthesis and provides a framework for future research...
  90. ncbi Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1
    Erwann Le Rouzic
    Institut Cochin, Department of Infectious Diseases, CNRS UMR8104, INSERM U567, Universite Paris 5, Paris, France
    J Biol Chem 277:45091-8. 2002
    ..This association of Vpr with a component of the nuclear pore complex may contribute to the disruption of the nuclear envelope and to the nuclear import of the viral DNA...
  91. ncbi A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
    X Cao
    The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
    Science 293:115-20. 2001
    ..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
  92. ncbi The nucleocapsid protein of the SARS coronavirus is capable of self-association through a C-terminal 209 amino acid interaction domain
    Milan Surjit
    Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Road, New Delhi 110067, India
    Biochem Biophys Res Commun 317:1030-6. 2004
    ..Furthermore, via deletion analysis, we have shown that the C-terminal 209 amino-acid region constitutes the interaction domain responsible for self-association of the N protein to form dimers...
  93. ncbi The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that interacts with DNA polymerase alpha
    G Crevel
    Department of Biochemistry and Immunology, St Georges Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK
    J Cell Sci 114:2015-25. 2001
    ..This provides the first suggestion of a role for chaperones in DNA replication in higher eukaryotes...
  94. pmc WormBase: a comprehensive data resource for Caenorhabditis biology and genomics
    Nansheng Chen
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nucleic Acids Res 33:D383-9. 2005
    ..Internally, we have restructured the database models to rationalize the representation of genes and to prepare the system to accept the genome sequences of three additional Caenorhabditis species over the coming year...
  95. pmc Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex
    N Kataoka
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
    EMBO J 20:6424-33. 2001
    ..These experiments thus reveal specific protein-protein interactions among the proteins of the splicing-dependent mRNP complex and suggest an important role for the highly evolutionarily conserved magoh protein in this complex...
  96. ncbi BZI-1 specifically heterodimerises with the tobacco bZIP transcription factors BZI-2, BZI-3/TBZF and BZI-4, and is functionally involved in flower development
    A Strathmann
    , , , , Germany
    Plant J 28:397-408. 2001
    ..Based on these data, we discuss a model postulating BZI transcription factor heterodimerisation as a mechanism determining target gene selection and regulating processes involved in plant development...
  97. ncbi Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response
    C Schwechheimer
    Science 292:1379-82. 2001
    ..Thus, the COP9 signalosome may play an important role in mediating E3 ubiquitin ligase-mediated responses...
  98. ncbi Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid systems
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Blood 102:136-41. 2003
    ..Direct interaction between FANCE and FANCD2 was also demonstrated in the yeast 2-hybrid system. This interaction involving an amino-terminal region of FANCD2 may provide a link between the FA protein complex and its downstream targets...
  99. ncbi The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectin
    L Fontao
    The Netherlands Cancer Institute, Division of Cell Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Cell Sci 114:2065-76. 2001
    ..We propose a new mechanism by which plectin and possibly also other actin-binding proteins can regulate the organization of the F-actin network in the cell...
  100. pmc Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1
    C Geiger
    Laboratorium für Molekulare Biologie, Genzentrum der Universität München, Germany
    EMBO J 19:2525-36. 2000
    ..We therefore propose that both direct regulation of the integrin and concomitant changes in the membrane topology of adherent T cells are modulated by dissectable functions of cytohesin-1...
  101. ncbi Nine-amino-acid transactivation domain: establishment and prediction utilities
    Simona Piskacek
    Simopharm, Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, A 1030 Vienna, Austria
    Genomics 89:756-68. 2007
    ..A computational search for the 9aa TAD is available online from National EMBnet-Node Austria at http://www.at.embnet.org/toolbox/9aatad/...

Research Grants63

  1. Regulation of Hepatic Lipid and Glucose Metabolism by PC-TP/StarD2
    David E Cohen; Fiscal Year: 2013
    ..These studies are expected to identify new therapeutic targets for the management of hepatic insulin resistance and related disorders. ..
  2. Phospholipid-Mediated Metabolic Control in Liver and Brown Adipose Tissue
    David E Cohen; Fiscal Year: 2013
    ..The proposed studies are relevant to the mission of the NIDDK because they are expected to identify new therapeutic targets for the management of common disorders related to diabetes and obesity. ..
  3. SGK Regulation of Epithelial Sodium Transport
    David Pearce; Fiscal Year: 2013
    ..C) Examine the role of mTOR in regulation of amiloride-inhibitable currents in isolated CCD using patch clamp. ..
  4. The Cutaneous Biology of MAGE Transcription Factors
    B Jack Longley; Fiscal Year: 2013
    ..KAP1- KRAB binding in a mammalian two hybrid assay, b. KAP1 mediated gene repression in a luciferase reporter gene assay, and c. KAP1 mediated KZNF ubiquitination by immunoblotting. ..
  5. Role of TGIF in Wnt-Induced Bone Formation
    Azeddine Atfi; Fiscal Year: 2013
    ....
  6. Elucidating the molecular function of Hph1 in regulating metal ion homeostasis
    Francisco Pina; Fiscal Year: 2009
    ..Defects in metal ion transport, storage, and/or utilization are associated with diseases, such as anemia and neurological, heart, lung and liver diseases. ..
  7. Structural and functional characterization of the Nbs1 N-terminus
    GERALD EDWARD DODSON; Fiscal Year: 2010
    ..Therefore, identification of novel factors and processes that regulate the function of Nbs1 and the MRN complex has the potential to neutralize multiple human diseases. ..
  8. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol S Lim; Fiscal Year: 2012
    ..Our long-term, ultimate goal is to use localization controllable versions of Bcr-Abl (as gene therapy) for treatment of CML. ..
  9. Mechanism of Mammalian Translesion DNA synthesis
    Masaaki Moriya; Fiscal Year: 2013
    ..Hence, it is very important to reveal the cellular mechanism by which unrepaired DNA damage exerts genotoxic effects, such as point mutations and chromosome aberrations, and causes cell death. ..
  10. REGULATION OF NORMAL AND DEFECTIVE HUMAN GENES
    Lynne E Maquat; Fiscal Year: 2013
    ..As an example, NMD provides a means by which many RNA binding proteins negatively regulate the genes from which they derived so that an appropriate level of gene expression can be achieved. ..
  11. Fleer: Functional study in regulation of vertebrate cilia tublin modification
    NARENDRA H PATHAK; Fiscal Year: 2012
    ..Success of the proposed research will allow us to identify specific, tubulin-glutamylation dependent processes associated with ciliopathies that may underlie human disease conditions. ..
  12. Hepatitis HBx-Mediated Pathogenesis
    BETTY SLAGLE; Fiscal Year: 2009
    ..Our studies will provide insight into the underlying mechanism by which HBx promotes virus replication, with the opportunity of identifying novel targets through which to disrupt ongoing viral replication. ..
  13. CELLULAR RESPONSE TO DNA ADDUCTS
    Masaaki Moriya; Fiscal Year: 2009
    ..abstract_text> ..
  14. Dissecting the molecular function of basigin a protein required for normal struct
    Kathryn D Curtin; Fiscal Year: 2010
    ..The project's main aim is to identify basigin-interacting proteins in the eye and to analyze the genes that code for these proteins so that we can better understand how basigin affects visual system development and function. ..
  15. Integrated Genomewide Analysis of the lpL1 Kinase Signaling Network
    Tony Hazbun; Fiscal Year: 2012
    ..A better understanding of these complicated protein networks will allow us to find therapies and design better drugs that would preferentially target cancerous cells. ..
  16. The Role of RECQL4 in Bone Development and Osteosarcoma
    Lisa L Wang; Fiscal Year: 2013
    ..This will allow us to discover novel ways to improve the treatment of osteosarcoma and osteoporosis in the general population. ..
  17. Roles of Lig3 and XRCC1 Genes in Genome Stability
    Alan E Tomkinson; Fiscal Year: 2013
    ..abstract_text> ..
  18. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2002
    ..Seventh, we will obtain new GCN4 derivatives that have altered DNA-binding specificity and design GCN4 mutants and target sites to test specific hypotheses that arise from the X-ray structure of the protein-DNA complex. ..
  19. A systems level analysis of spore coat assembly in Bacillus subtilis
    Patrick Eichenberger; Fiscal Year: 2012
    ..We anticipate that our results will have an impact on the design of therapeutic approaches to detect and eliminate pathogenic spore-forming bacteria. ..
  20. Technology for isolation of Integrase Inhibitors for Therapeutics against Human I
    TANYA MARIE SANDROCK; Fiscal Year: 2010
    ..Compounds isolated that possess desirable inhibitory properties will be optimized for further pre-clinical studies. ..
  21. Developing methods for proximity-dependent protein labeling
    Kyle J Roux; Fiscal Year: 2013
    ..This will enhance the efficiency of application of BioD to a broad array of projects relevant to human health and disease. ..
  22. INTESTINAL & PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 2000
    ..Lastly, the proposed work almost completes the studies that follow directly from the discovery by the P.I. of MHC class I-related FcRs, and additional time will be essential to consider and plan future research directions. ..
  23. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2006
    ..Furthermore, since homologues of N. crassa G-alpha genes are required for virulence in numerous fungal species, these investigations will also lead to new therapies for emerging fungal pathogens. ..
  24. Functional Analysis of an hnRNP C-Like Protein, p542
    JAMES MCAFEE; Fiscal Year: 2004
    ..The in-vitro telomere binding specificity of p542, parallels inferences linking hnRNP C to telomeres, and prompts our investigations into the in vivo association of p542 with telomeric DNA. ..
  25. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2006
    ..abstract_text> ..
  26. Assigning mechanism to human polymorphisms via interacting residue identification
    Murat Cokol; Fiscal Year: 2010
    ..These identified variations will offer insights for finding the causes of diseaes and also add a new dimension to our understanding of the relationship between genetic variation and cellular networks. ..
  27. Characterization of Chlamydial Inclusion Migration
    Scott Grieshaber; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  28. DIMERIZATION & COOPERATIVITY IN PROKARYOTIC GENE CONTROL
    Ann Hochschild; Fiscal Year: 2002
    ..This assay will also be applied to the study and manipulation of a phosphorylation-dependent protein-protein interaction involving the eukaryotic transcription factor CREB and its CREB and its co-activator CBP. ..
  29. C/EBP ALPHA MEDIATED REGULATION OF P21/SDI-1
    Nikolai Timchenko; Fiscal Year: 2001
    ..For this goal we will clone the genes that are responsible for C/EBPalpha-mediated growth arrest. ..
  30. JUN Kinase Signaling in the Lung
    Glenn Rosen; Fiscal Year: 2006
    ..These studies will elucidate the biological function of a novel and unique regulator of Ras signal transduction. ..
  31. HYPOHIDROTIC ECTODERMAL DYSPLASIA--A GENETIC ANALYSIS
    Jonathan Zonana; Fiscal Year: 2002
    ..At the same time, the new knowledge should rapidly result in improved genetic diagnosis and counseling, and may even suggest avenues for future therapies. ..
  32. Modulation of Electrogenic Sodium Bicarbonate Transport
    Ira Kurtz; Fiscal Year: 2007
    ..Successful completion of this project will enhance our understanding of the mechanisms responsible for regulating H+/base transporters. ..
  33. CHARACTERIZATION OF NOVEL G-ALPHA INTERACTING PROTEINS
    Kim Neve; Fiscal Year: 2001
    ..3) Functional consequences of physical interactions, confirmed in aim l, between G-alpha and any of the other three target proteins will be determined. ..
  34. Role of band 3 in Malaria Invasion of Red Blood Cells
    Athar Chishti; Fiscal Year: 2007
    ..falciparum lines. Together, a clear understanding of the sialic acid-independent invasion mechanism will provide valuable insights into the development of an effective subunit malaria vaccine. ..
  35. Functional analysis of Helicobacter pylori VacA toxin
    Victor Torres; Fiscal Year: 2003
    ..To fulfill this aim I am using the VacA recombinant system to determine the minimum amino acid sequence required for the delta6-27-VacA toxin to exhibit a dominant-negative phenotype. ..
  36. ACTIVATION MECHANISMS IN THE NADPH OXIDASE
    Michael Kleinberg; Fiscal Year: 2003
    ..These findings may lead to new strategies for design of novel anti-inflammatory agents that will inhibit oxidase formation and production of superoxide by phagocytes. ..
  37. Mechanisms of Glomerular Scarring
    John Sedor; Fiscal Year: 2006
    ..In vitro and in vivo experiments will test the validity of our model. ..
  38. FUNCTION OF THE RHEB G-PROTEIN IN ASPERGILLUS FUMIGATUS
    JUDITH RHODES; Fiscal Year: 2004
    ..Completion of these aims should significantly clarify the function of rhbA in A. fumigatus. ..
  39. Structure Function in Low Resistance Junctions
    Camillo Peracchia; Fiscal Year: 2005
    ..The relevance of a potential CaM binding site identified in connexins (similar to that of CaM kinase II) will be tested with deletion and mutation of crucial Cx32 residues. ..
  40. CONJUGATIVE MOBILIZATION OF PLASMID R1162
    Richard Meyer; Fiscal Year: 2003
    ..How pSCl0l has become independent of these proteins will investigated. Overall, these studies will provide insight into how the relaxosome directs plasmid DNA to the conjugal pore for transfer. ..
  41. PHOSPHOLIPASE C AND ISLET BETA CELLS
    GRANT KELLEY; Fiscal Year: 2002
    ..This novel signaling pathway may play a critical role in glucose and hormone stimulated insulin secretion and beta cell function. ..
  42. FUNCTIONAL STUDIES OF TOPOISOMERASE I
    Eric Rubin; Fiscal Year: 2002
    ..Collectively, these studies should provide information valuable not only for understanding the cellular role of topoisomerase I but also for elucidating the cytotoxic mechanisms of camptothecin. ..
  43. The enhancer of rudimentary gene
    STUART TSUBOTA; Fiscal Year: 2002
    ..The expression data of e(r) suggest that mutants may be embryonic lethals. If this is the case, the mutants will be analyzed for cellular defects in the embryo that that will help uncover the normal function of e(r). ..
  44. MOLECULAR GENETICS OF C ELEGANS RENAL TUBULES
    Matthew Buechner; Fiscal Year: 2002
    ....
  45. TRANSGENIC ANALYSIS OF LH HYPERSECRETION
    John Nilson; Fiscal Year: 1999
    ....
  46. MOLECULAR BASIS OF RETINOBLASTOMA FORMATION
    Wen Hwa Lee; Fiscal Year: 2000
    ..Results of these studies will contribute significantly to a basic understanding of the genesis of retinoblastoma in particular and human oncogenesis in general. ..
  47. Glutamine:Fructose-6-phosphate Amidotransferase forNIDDM
    David Teng; Fiscal Year: 2002
    ..If so, the peptide binders themselves may be useful in a high-throughput screen involving the displacement of the peptide binder from hsGFA1 by a small molecule. ..
  48. SMALL GTPASES AND LUNG BETA RECEPTOR REGULATION
    BRIAN KNOLL; Fiscal Year: 2002
    ..These studies will provide insight into the mechanisms of endocytic trafficking events, and how they regulate the activity and number of an important signal-transducing receptor. ..
  49. RECEPTOR LINKED PROTEIN TYROSINE PHOSPHATASES
    Haruo Saito; Fiscal Year: 2002
    ..The structures of phosphatase-substrate complexes will be determined. Also, the structural basis of the catalytic and regulatory functions of the individual PTPase domains in LAR will be investigated. ..
  50. MOLECULAR BASIS OF ACUTE PROMYELOCYTIC LEUKEMIA
    J Don Chen; Fiscal Year: 2004
    ..A better understanding of mechanism of APL at the molecular and cellular levels should lead to a more effective diagnosis and treatment of this disease, as well as providing implications for other malignancies. ..
  51. Regulation of Macrophage Activation and Apoptosis
    Cun Yu Wang; Fiscal Year: 2004
    ..In summary, these studies will provide new insights into the mechanisms of macrophage activation and apoptosis, and have important implication in treatment of oral an systemic inflammatory diseases and septic shock. ..
  52. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..The knowledge will then be applied to the question of how the deregulation of the checkpoint function may contribute to cancer progression. ..
  53. HIV Nef Ubiquitination and Its Functions
    Yong Jiu Jin; Fiscal Year: 2009
    ..Our discovery of Nef ubiquitination will open up a new field of HIV biology research and provide a new specific target for anti-HIV therapy. ..
  54. Genetic analysis of G protein signaling in S. pombe
    FRANK IVEY; Fiscal Year: 2005
    ..pombe to control gene expression promises to not only advance and challenge our current knowledge of how cells respond to changing environments but, could potentially uncover medically important therapeutic targets. ..
  55. BETA CATENIN IN PROSTATE CANCER
    Edward Gelmann; Fiscal Year: 2005
    ..abstract_text> ..
  56. MODULATION OF TRIGEMINAL INHIBITION
    Li Yen Huang; Fiscal Year: 2004
    ..A better understanding of the mechanism of GlyR modulation will provide important information about the neuronal signaling and may lead to new therapy for orofacial pain. ..
  57. Characterization of Arabidopsis peroxins acting in matrix protein import
    NAXHIELY MARTINEZ; Fiscal Year: 2009
    ....
  58. Molecular Biology of 5HT2A receptor expressing synapses
    Milt Teitler; Fiscal Year: 2005
    ....
  59. Study of a Cannabinoid Receptor Interacting Protein
    JASON NIEHAUS; Fiscal Year: 2004
    ..In addition, further insight into the cannabinoid system may lead to a better understanding of marijuana abuse, tolerance and dependence. ..
  60. A TWO-HYBRID SYSTEM FOR USE IN NON-DIVIDING YEAST CELL
    Margaret Werner Washburne; Fiscal Year: 2002
    ..Once established for yeast, this method should be useful in a broad range of cell types to facilitate the genomic-scale analysis of protein-protein interactions during all phases of growth. ..
  61. TG737 SIGNALING PATHWAY AND POLYCYSTIC KIDNEY
    Bradley Yoder; Fiscal Year: 2003
    ..Overall, results generated from this proposal will give important insight into the biological function of Tg737 and how disruption of the Tg737/polycystin-1 signaling pathway results in the formation of renal cyst. ..
  62. ION CHANNELS IN THE NERVOUS SYSTEM
    John Caldwell; Fiscal Year: 2003
    ..It is expected that mutations in brain sodium channels and in the proteins that bind to NaChs will produce CNS disorders in humans, and the proposed studies will contribute to our understanding of these disorders. ..
  63. New Proteins Binding Apoptotic Stimulator of p53, ASPP2
    LARRY ROHDE; Fiscal Year: 2004
    ..abstract_text> ..