two hybrid system techniques

Summary

Summary: Screening techniques used to identify genes encoding interacting proteins. Variations are used to evaluate complex interplay between proteins and other molecules.

Top Publications

  1. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
  2. ncbi NoBP, a nuclear fibroblast growth factor 3 binding protein, is cell cycle regulated and promotes cell growth
    K Reimers
    Institut für Hämostaseologie und Transfusionsmedizin, Medizinische Fakultat, Heinrich Heine Universitat, D 40225 Dusseldorf, Germany
    Mol Cell Biol 21:4996-5007. 2001
  3. ncbi Evaluation of clustering algorithms for protein-protein interaction networks
    Sylvain Brohée
    Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
    BMC Bioinformatics 7:488. 2006
  4. ncbi Vaccinia virus DNA ligase recruits cellular topoisomerase II to sites of viral replication and assembly
    Y C James Lin
    Department of Medical Microbiology and Immunology, Alberta Institute for Viral Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Virol 82:5922-32. 2008
  5. ncbi A rice kinase-protein interaction map
    Xiaodong Ding
    Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 149:1478-92. 2009
  6. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
  7. ncbi High-quality binary protein interaction map of the yeast interactome network
    Haiyuan Yu
    Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 322:104-10. 2008
  8. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
  9. ncbi A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 125:801-14. 2006
  10. ncbi The tripartite motif family identifies cell compartments
    A Reymond
    Telethon Institute of Genetics and Medicine TIGEM, San Raffaele Biomedical Science Park, Milan, Italy
    EMBO J 20:2140-51. 2001

Research Grants

  1. Mechanisms of HIV-1 Variation
    LOUIS MANSKY; Fiscal Year: 2003
  2. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2005
  3. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2007
  4. INTESTINAL AND PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 1999
  5. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2003
  6. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2006
  7. PROTEINS INVOLVED IN TRANSPORT THROUGH PLASMODESMATA
    Vitaly Citovsky; Fiscal Year: 2000
  8. Functional Analysis of an hnRNP C-Like Protein, p542
    JAMES MCAFEE; Fiscal Year: 2004
  9. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol S Lim; Fiscal Year: 2010
  10. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol Lim; Fiscal Year: 2009

Detail Information

Publications241 found, 100 shown here

  1. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
    ..We propose that the small G-protein kir/Gem, interacting with beta-subunits, regulates Ca2+ channel expression at the cell surface...
  2. ncbi NoBP, a nuclear fibroblast growth factor 3 binding protein, is cell cycle regulated and promotes cell growth
    K Reimers
    Institut für Hämostaseologie und Transfusionsmedizin, Medizinische Fakultat, Heinrich Heine Universitat, D 40225 Dusseldorf, Germany
    Mol Cell Biol 21:4996-5007. 2001
    ..We propose that NoBP is the functional target of nuclear FGF3 action...
  3. ncbi Evaluation of clustering algorithms for protein-protein interaction networks
    Sylvain Brohée
    Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
    BMC Bioinformatics 7:488. 2006
    ..In this paper we present a comparative assessment of four algorithms: Markov Clustering (MCL), Restricted Neighborhood Search Clustering (RNSC), Super Paramagnetic Clustering (SPC), and Molecular Complex Detection (MCODE)...
  4. ncbi Vaccinia virus DNA ligase recruits cellular topoisomerase II to sites of viral replication and assembly
    Y C James Lin
    Department of Medical Microbiology and Immunology, Alberta Institute for Viral Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Virol 82:5922-32. 2008
    ..Although other nuclear DNA binding proteins have been detected in virosomes, this appears to be the first demonstration of an enzyme being selectively recruited to sites of poxvirus DNA synthesis and assembly...
  5. ncbi A rice kinase-protein interaction map
    Xiaodong Ding
    Department of Plant Pathology, University of Florida, Gainesville, Florida 32611, USA
    Plant Physiol 149:1478-92. 2009
    ..The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed...
  6. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
    ..This effect decreases the likelihood of cross talk between different functional modules of the cell and increases the overall robustness of a network by localizing effects of deleterious perturbations...
  7. ncbi High-quality binary protein interaction map of the yeast interactome network
    Haiyuan Yu
    Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 322:104-10. 2008
    ..Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy...
  8. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies...
  9. ncbi A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 125:801-14. 2006
    ..This interactome thus provides a tool for understanding pathogenic mechanisms common for this class of neurodegenerative disorders and for identifying candidate genes for inherited ataxias...
  10. ncbi The tripartite motif family identifies cell compartments
    A Reymond
    Telethon Institute of Genetics and Medicine TIGEM, San Raffaele Biomedical Science Park, Milan, Italy
    EMBO J 20:2140-51. 2001
    ..A total of 37 TRIM genes/proteins were studied, 21 of which were novel. The results demonstrate that TRIM proteins share a common function: by means of homo-multimerization they identify specific cell compartments...
  11. ncbi Sensing of Lys 63-linked polyubiquitination by NEMO is a key event in NF-kappaB activation [corrected]
    Chuan Jin Wu
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 8:398-406. 2006
    ..These results provide a mechanism for NEMO's critical role in IKK activation, and a key to understanding the link between cytokine-receptor proximal signalling and IKK and NF-kappaB activation...
  12. ncbi Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK
    Chie Kanei-Ishii
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305 0074, Japan
    Genes Dev 18:816-29. 2004
    ..The down-regulation of Myb by Wnt-1 signal may play an important role in a variety of developmental steps...
  13. ncbi Group 13 HOX proteins interact with the MH2 domain of R-Smads and modulate Smad transcriptional activation functions independent of HOX DNA-binding capability
    Thomas M Williams
    Department of Human Genetics, University of Michigan Ann Arbor, MI, USA
    Nucleic Acids Res 33:4475-84. 2005
    ....
  14. ncbi PHD domain-mediated E3 ligase activity directs intramolecular sumoylation of an adjacent bromodomain required for gene silencing
    Alexey V Ivanov
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell 28:823-37. 2007
    ..These data provide a mechanistic explanation for the cooperation of PHD and bromodomains in gene regulation and describe a function of the PHD domain as an intramolecular E3 SUMO ligase...
  15. ncbi Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis
    Gouzel Karimova
    Unité de Biochimie des Interactions Macromoléculaires, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Bacteriol 187:2233-43. 2005
    ..In addition, our study shows that the cAMP-based two-hybrid system is particularly appropriate for analyzing molecular interactions between membrane proteins...
  16. ncbi Matrix and Steiner-triple-system smart pooling assays for high-performance transcription regulatory network mapping
    Vanessa Vermeirssen
    Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Methods 4:659-64. 2007
    ..Both strategies outperform library screens in terms of coverage, confidence and throughput. These versatile strategies can be adapted both to TFs in other systems and, likely, to other biomolecules and assays as well...
  17. ncbi Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74
    Christian Schwarzer
    Max Planck Institute for Experimental Medicine, Department of Immunochemistry, Hermann Rein Street 3, 37075 Gottingen, Germany
    Int J Biochem Cell Biol 34:1059-70. 2002
    ....
  18. ncbi Specific interaction of the potassium channel beta-subunit minK with the sarcomeric protein T-cap suggests a T-tubule-myofibril linking system
    T Furukawa
    Department of Physiology, Akita University School of Medicine, Japan
    J Mol Biol 313:775-84. 2001
    ..We speculate that this interaction may contribute to a stretch-dependent regulation of potassium flux in cardiac muscle, providing a "mechano-electrical feedback" system...
  19. ncbi A protein interaction network of the malaria parasite Plasmodium falciparum
    Douglas J LaCount
    Howard Hughes Medical Institute, University of Washington, Box 357730, Seattle, Washington 98195, USA
    Nature 438:103-7. 2005
    ..These data constitute the first extensive description of the protein interaction network for this important human pathogen...
  20. ncbi Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2
    Stephan Lange
    Institute of Cell Biology, ETH Honggerberg, 8093 Zurich, Switzerland
    J Cell Sci 115:4925-36. 2002
    ..We propose that DRAL/FHL-2 acts as a specific adaptor protein to couple metabolic enzymes to sites of high energy consumption in the cardiac sarcomere...
  21. ncbi Annexin II light chain regulates sensory neuron-specific sodium channel expression
    Kenji Okuse
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Nature 417:653-6. 2002
    ..Because direct association with p11 is required for functional expression of Na(V)1.8, disrupting this interaction may be a useful new approach to downregulating Na(V)1.8 and effecting analgesia...
  22. ncbi Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex
    Leila Alland
    Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 22:2743-50. 2002
    ....
  23. ncbi The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex
    Beatriz Aranda-Orgilles
    Max Planck Institute for Molecular Genetics, Ihnestr 73, 14195, Berlin, Germany
    Hum Genet 123:163-76. 2008
    ..The dysfunction of this mechanism would lead to malfunction of microtubule-associated protein translation and to the development of OS...
  24. ncbi Human Vam6p promotes lysosome clustering and fusion in vivo
    S Caplan
    Cell Biology and Metabolism Branch at the National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 154:109-22. 2001
    ..This study implicates hVam6p as a mammalian tethering/docking factor characterized with intrinsic ability to promote lysosome fusion in vivo...
  25. ncbi Negative regulation of the mammalian UV response by Myc through association with Miz-1
    Steffi Herold
    Institute for Molecular Biology and Tumor Research, 35033 Marburg, Germany
    Mol Cell 10:509-21. 2002
    ..Our data implicate Miz-1 in a pathway that regulates cell proliferation in response to UV irradiation...
  26. ncbi Mammalian 14-3-3beta associates with the Chlamydia trachomatis inclusion membrane via its interaction with IncG
    M A Scidmore
    Host Parasite Interactions Section, Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Mol Microbiol 39:1638-50. 2001
    ..psittaci or C. pneumoniae inclusions. 14-3-3beta is the first eukaryotic protein found to interact with the chlamydial inclusion; however, its unique role in C. trachomatis pathogenesis remains to be determined...
  27. ncbi Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain
    T Centner
    European Molecular Biology Laboratory, Heidelberg, D 69117, Germany
    J Mol Biol 306:717-26. 2001
    ....
  28. ncbi Abscisic acid signaling in seeds and seedlings
    Ruth R Finkelstein
    Department of Molecular, Cellular, and Developmental Biology, University of California at Santa Barbara, Santa Barbara, CA 93106, USA
    Plant Cell 14:S15-45. 2002
  29. ncbi Myotilin is mutated in limb girdle muscular dystrophy 1A
    M A Hauser
    Department of Medicine, Section of Medical Genetics, Duke University Medical Center, DUMC 3445, Durham, NC 27710 3445, USA
    Hum Mol Genet 9:2141-7. 2000
    ..Myotilin is a sarcomeric protein that binds to alpha-actinin and is localized in the Z-line. The observed missense mutation does not disrupt binding to alpha-actinin...
  30. ncbi Conserved patterns of protein interaction in multiple species
    Roded Sharan
    Computer Science Division, University of California, Berkeley, CA 94704, USA
    Proc Natl Acad Sci U S A 102:1974-9. 2005
    ....
  31. ncbi Comprehensive interaction map of the Arabidopsis MADS Box transcription factors
    Stefan de Folter
    Business Unit Bioscience, Plant Research International, 6700 AA Wageningen, The Netherlands
    Plant Cell 17:1424-33. 2005
    ..Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower...
  32. ncbi The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein
    Isabelle Imbert
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, Marseille Cedex 9, France
    Virus Res 133:136-48. 2008
    ..The interaction map thus provides a sum of the interactions that may occur at some point during coronavirus RNA synthesis and provides a framework for future research...
  33. ncbi Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein
    K Yeung
    Department of Molecular Biology, Brown University, Providence, Rhode Island 02912, USA
    Mol Cell Biol 20:3079-85. 2000
    ..The properties of RKIP reveal the specific sequestration of interacting components as a novel motif in the cell's repertoire for the regulation of signaling pathways...
  34. ncbi Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome
    Joy Armistead
    Department of Biochemistry and Medical Genetics, University of Manitoba, MB, Canada
    Am J Hum Genet 84:728-39. 2009
    ..These findings suggested that the D-to-G mutation caused aggregation of EMG1, thereby reducing the level of the protein and causing BCS...
  35. ncbi A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
    X Cao
    The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
    Science 293:115-20. 2001
    ..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
  36. ncbi Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1
    Erwann Le Rouzic
    Institut Cochin, Department of Infectious Diseases, CNRS UMR8104, INSERM U567, , Paris, France
    J Biol Chem 277:45091-8. 2002
    ..This association of Vpr with a component of the nuclear pore complex may contribute to the disruption of the nuclear envelope and to the nuclear import of the viral DNA...
  37. ncbi A root-knot nematode secretory peptide functions as a ligand for a plant transcription factor
    Guozhong Huang
    Department of Plant Pathology, University of Georgia, Athens 30602 7274, USA
    Mol Plant Microbe Interact 19:463-70. 2006
    ..This discovery is the first demonstration of a direct interaction of a nematode-secreted parasitism peptide with a plant-regulatory protein, which may represent an early signaling event in the root-knot nematode-host interaction...
  38. ncbi Interaction of c-Jun amino-terminal kinase interacting protein-1 with p190 rhoGEF and its localization in differentiated neurons
    D Meyer
    Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 274:35113-8. 1999
    ..The interaction of JIP-1 with rhoGEF and its subcellular localization suggests that JIP-1 may function to specifically localize a signaling complex in neuronal cells...
  39. ncbi Biochemistry. Not comparable, but complementary
    Lars Juhl Jensen
    European Molecular Biology Laboratory, D-69117 Heidelberg, Germany
    Science 322:56-7. 2008
  40. ncbi The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif
    Sophie Deltour
    CNRS UMR 8526, Institut de Biologie de Lille, Institut Pasteur de Lille, 59017 Lille Cedex, France
    Mol Cell Biol 22:4890-901. 2002
    ..In conclusion, our results demonstrate that HIC1 mediates transcriptional repression by both HDAC-independent and HDAC-dependent mechanisms and show that CtBP is a HIC1 corepressor that is recruited via a variant binding site...
  41. ncbi Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors
    A Kiss
    Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
    Virology 314:591-600. 2003
    ..These results suggest a physiological role for hNup98 in modulating Rev-dependent RNA export during HIV infection...
  42. ncbi A large-scale protein protein interaction analysis in Synechocystis sp. PCC6803
    Shusei Sato
    Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
    DNA Res 14:207-16. 2007
    ..The interaction data obtained in this study should provide new insights and novel strategies for functional analyses of genes in Synechocystis, and, additionally, genes in other cyanobacteria and plant genes of cyanobacterial origin...
  43. ncbi NDR1 interaction with RIN4 mediates the differential activation of multiple disease resistance pathways in Arabidopsis
    Brad Day
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94270, USA
    Plant Cell 18:2782-91. 2006
    ..We suggest that this interaction serves to further regulate activation of disease resistance signaling following recognition of P. syringae DC3000-AvrRpt2 by Arabidopsis...
  44. ncbi Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins
    Katsumi Kasashima
    Department of Biology, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    Nucleic Acids Res 30:4519-26. 2002
    ..These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs...
  45. ncbi CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription
    Linyu Li
    Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology and School of Medicine, Tsinghua University, Beijing 100084, People s Republic of China
    Mol Cell Biol 24:856-64. 2004
    ..These results suggest that CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins...
  46. ncbi LeSTIG1, an extracellular binding partner for the pollen receptor kinases LePRK1 and LePRK2, promotes pollen tube growth in vitro
    Weihua Tang
    Plant Gene Expression Center, United States Department of Agriculture Agricultural Research Service, and Department of Plant and Microbial Biology, University of California at Berkeley, 800 Buchanan Street, Albany, CA 94710, USA
    Plant J 39:343-53. 2004
    ..Exogenous LeSTIG1 promotes pollen tube growth in vitro. The interaction of these pollen kinases with LeSTIG1 supports the notion that LePRK1 and LePRK2 are involved in mediating pollen-pistil interactions...
  47. ncbi Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases
    Hitoshi Yoshida
    Department of Rice Research, National Agricultural Research Center, Jo etsu, Niigata 943 0193, Japan
    BMC Plant Biol 5:14. 2005
    ..Because orthologous sequences to ETO1 are found in many plant species including tomato, we transformed tomato with Arabidopsis ETO1 to evaluate its ability to suppress ethylene production in tomato fruits...
  48. ncbi TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus
    Nicolas Le May
    Unité de Génétique Moléculaire des Bunyaviridés, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Cell 116:541-50. 2004
    ..These observations shed light on the mechanism utilized by RVFV to evade the host response...
  49. ncbi The YPWM motif links Antennapedia to the basal transcriptional machinery
    Frederic Prince
    Biozentrum, University of Basel, Klingelberstrasse 70, CH 4056 Basel, Switzerland
    Development 135:1669-79. 2008
    ..BIP2 is a TATA-binding protein associated factor (also known as dTAFII3) that links ANTP to the basal transcriptional machinery...
  50. ncbi HIV-1 Vpr-mediated G2 arrest involves the DDB1-CUL4AVPRBP E3 ubiquitin ligase
    Jean Philippe Belzile
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada
    PLoS Pathog 3:e85. 2007
    ....
  51. ncbi Domain II mutations in CRANE/IAA18 suppress lateral root formation and affect shoot development in Arabidopsis thaliana
    Takeo Uehara
    Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai, Kobe, 657 8501 Japan
    Plant Cell Physiol 49:1025-38. 2008
    ..Taken together, our results indicate that CRANE/IAA18 is involved in lateral root formation in Arabidopsis, and suggest that it negatively regulates the activity of ARF7 and ARF19 for lateral root formation...
  52. ncbi The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting
    Keiichi Katoh
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    J Biol Chem 278:39104-13. 2003
    ..These results suggest that CHMP4b and Alix participate in formation of multivesicular bodies by cooperating with SKD1...
  53. ncbi MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana
    Kiyoshi Tatematsu
    Division of Biological Sciences, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Plant Cell 16:379-93. 2004
    ..These results suggest that MSG2/IAA19 and NPH4/ARF7 may constitute a negative feedback loop to regulate differential growth responses of hypocotyls and lateral root formation...
  54. ncbi The formin-homology protein SmDia interacts with the Src kinase SmTK and the GTPase SmRho1 in the gonads of Schistosoma mansoni
    Thomas Quack
    Institute for Parasitology, Justus Liebig University, Giessen, Germany
    PLoS ONE 4:e6998. 2009
    ..Since Src kinases are important signal transduction proteins it is of interest to unravel the signaling cascades SmTK3 is involved in to understand its cellular role in the gonads...
  55. ncbi Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex
    S Krugmann
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, CRC Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Curr Biol 11:1645-55. 2001
    ..Cdc42 controls the formation of actin bundle-containing filopodia at the cellular periphery. The molecular mechanism for this remains as yet unclear...
  56. ncbi A pathway for association of receptors, adaptors, and actin during endocytic internalization
    Marko Kaksonen
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 115:475-87. 2003
    ..Attention is now focused on the mechanisms by which the order and timing of events in this endocytic pathway are achieved...
  57. ncbi Characterization of a carboxy-terminal BRCA1 interacting protein
    A K Wong
    Myriad Genetics, Inc, Salt Lake City, Utah 84108, USA
    Oncogene 17:2279-85. 1998
    ..Thus it is plausible that CtIP may itself be a tumor suppressor acting in the same pathway as BRCA1...
  58. ncbi A receptor for activated C kinase is part of messenger ribonucleoprotein complexes associated with polyA-mRNAs in neurons
    Frank Angenstein
    Beckman Institute Neuronal Pattern Analysis, University of Illinois, Urbana, Illinois 61801, USA
    J Neurosci 22:8827-37. 2002
    ....
  59. ncbi Nine-amino-acid transactivation domain: establishment and prediction utilities
    Simona Piskacek
    Simopharm, Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, A 1030 Vienna, Austria
    Genomics 89:756-68. 2007
    ..A computational search for the 9aa TAD is available online from National EMBnet-Node Austria at http://www.at.embnet.org/toolbox/9aatad/...
  60. ncbi SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  61. ncbi A tctex1-Ca2+ channel complex for selective surface expression of Ca2+ channels in neurons
    Meizan Lai
    Department of Pharmacology, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104, USA
    Nat Neurosci 8:435-42. 2005
    ..These results underscore the importance of the specific tctex1-channel interaction in determining sorting and trafficking of neuronal Ca(2+) channels with different functionalities...
  62. ncbi Esc4/Rtt107 and the control of recombination during replication
    Jodie K Chin
    Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    DNA Repair (Amst) 5:618-28. 2006
    ..Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis...
  63. ncbi Insights into subunit interactions in the heterotetrameric structure of potato ADP-glucose pyrophosphorylase
    Aytug Tuncel
    Center for Computational Biology and Bioinformatics, and Department of Chemical and Biological Engineering and College of Engineering, Koc University, Istanbul, Turkey
    Biophys J 95:3628-39. 2008
    ..We further determined the order of assembly during the heterotetrameric structure formation. First, side-by-side LS-SS dimers form followed by the up-down tetramerization based on the relative binding free energies...
  64. ncbi Cpc2, a fission yeast homologue of mammalian RACK1 protein, interacts with Ran1 (Pat1) kinase To regulate cell cycle progression and meiotic development
    M McLeod
    State University of New York Health Science Center at Brooklyn, Department of Microbiology and Immunology, Morse Institute for Molecular Biology and Genetics, Brooklyn, New York 11203, USA
    Mol Cell Biol 20:4016-27. 2000
    ..All defects associated with loss of cpc2 are reversed in cells expressing mammalian RACK1, demonstrating that the fission yeast and mammalian gene products are indeed functional homologues...
  65. ncbi YPXL/I is a protein interaction motif recognized by aspergillus PalA and its human homologue, AIP1/Alix
    Olivier Vincent
    Departamento de Microbiologia Molecular, Centro de Investigaciones Biológicas del CSIC, Madrid 28006, Spain
    Mol Cell Biol 23:1647-55. 2003
    ....
  66. ncbi A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast
    Paul J Cullen
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genes Dev 18:1695-708. 2004
    ..Taken together, our data suggest that Msb2 is a signaling mucin that interacts with general components, such as Cdc42 and Sho1, to promote their function in the FG pathway...
  67. ncbi Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone
    Rongmin Zhao
    Department of Biochemistry, Medical Sciences Building, 1 King's College Circle, University of Toronto, Toronto, ON, M5S 1A8, Canada
    Cell 120:715-27. 2005
    ..These cofactors interact physically and functionally with the conserved AAA(+)-type DNA helicases Rvb1/Rvb2, which are key components of several chromatin remodeling factors, thereby linking Hsp90 to epigenetic gene regulation...
  68. ncbi Sorting nexin 6, a novel SNX, interacts with the transforming growth factor-beta family of receptor serine-threonine kinases
    W T Parks
    Laboratory of Cell Regulation and Carcinogenesis, NCI, the Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:19332-9. 2001
    ..These findings are the first evidence for the association of the SNX family of molecules with receptor serine-threonine kinases...
  69. ncbi Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16
    Zhibing Zhang
    Center for Research on Reproduction and Women s Health and the Department of Ophthalmology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Proteomics 4:914-23. 2005
    ..Thus, PF6, SPAG6, and PF20 form a newly identified network that links together components of the axoneme central apparatus and presumably participates in its dynamic regulation of ciliary and flagellar beat...
  70. ncbi TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis
    Heidi Szemenyei
    Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 319:1384-6. 2008
    ..These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development...
  71. ncbi Ubc9 is a novel modulator of the induction properties of glucocorticoid receptors
    Sunil Kaul
    Steroid Hormones Section, NIDDK Laboratory of Molecular and Cellular Biology, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 277:12541-9. 2002
    ..With high concentrations of Ubc9 and GR, Ubc9 binding to GR appears to be sufficient to permit Ubc9 to act independently of the GME...
  72. ncbi Interactions with Costal2 and suppressor of fused regulate nuclear translocation and activity of cubitus interruptus
    G Wang
    Center for Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9133, USA
    Genes Dev 14:2893-905. 2000
    ..Finally, we show that Cos2 is required for transducing high levels of Hh signaling activity, and it does so by alleviating the blockage of Ci activity imposed by Su(fu)...
  73. ncbi A protein domain-based interactome network for C. elegans early embryogenesis
    Mike Boxem
    Center for Cancer Systems Biology and Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 134:534-45. 2008
    ..This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms...
  74. ncbi A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening
    M Gunther
    Laboratoire de génétique oncologique, UMR 1599 CNRS, Institut Gustave Roussy, France
    Mol Cell Biochem 210:131-42. 2000
    ..Four other cDNA clones encoding polypeptides of unknown function were tested in the in vitro binding assay. All four polypeptides were found to interact with Sp1 in this assay...
  75. ncbi TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation
    R Perlman
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 3:708-14. 2001
    ..Our findings associate Daxx directly with the TGF-beta apoptotic-signalling pathway, and make a biochemical connection between the receptors for TGF-beta and the apoptotic machinery...
  76. ncbi Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast
    Andre P Gerber
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E79. 2004
    ....
  77. ncbi Evidence for simultaneous protein interactions between human Rad51 paralogs
    D Schild
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    J Biol Chem 275:16443-9. 2000
    ..These results suggest that these proteins may participate in one complex or multiple smaller ones...
  78. ncbi Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe
    Morgan Brown
    Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
    DNA Repair (Amst) 1:869-80. 2002
    ..Hence, our data strongly support the hypothesis that the PRR function mediated by UBC13-MMS2 is conserved throughout eukaryotes...
  79. ncbi Increasing specificity in high-throughput yeast two-hybrid experiments
    Pierre-Olivier Vidalain
    Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Methods 32:363-70. 2004
    ..Contaminating plasmids are eliminated by extended culture of yeast cells under positive selection for the interaction, allowing the identification of the true interaction partner...
  80. ncbi Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking
    Jianbo Yang
    Department of Laboratory Medicine and Pathology and Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 277:30219-26. 2002
    ..Our data suggest that Etk may be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1...
  81. ncbi A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance
    Manu Agarwal
    Institute for Integrative Genome Biology and Department of Botany and Plant Science, University of California, Riverside, California 92521, USA
    J Biol Chem 281:37636-45. 2006
    ..Our results suggest that MYB15 is part of a complex network of transcription factors controlling the expression of CBFs and other genes in response to cold stress...
  82. ncbi Physical and genetic interaction of filamin with presenilin in Drosophila
    Y Guo
    Program in Developmental Biology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8
    J Cell Sci 113:3499-508. 2000
    ..Taken together, these results suggest that presenilin and filamin functionally interact during development...
  83. ncbi PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway
    Xi Ma
    Department of Immunology, School of Basic Medicine, and Human Disease Genomics Center, Peking University, Beijing 100083, China
    Mol Biol Cell 18:1965-78. 2007
    ....
  84. ncbi MICAL-1 isoforms, novel rab1 interacting proteins
    Thomas Weide
    Department of Experimental Tumorbiology, University of Muenster, Badestr 9, D 48149, Muenster, Germany
    Biochem Biophys Res Commun 306:79-86. 2003
    ..We suggest that MICAL-1 isoforms with their multidomain structure are novel rab1 interacting proteins that function as scaffold proteins connecting different components in the cell...
  85. ncbi A bacterial effector targets Mad2L2, an APC inhibitor, to modulate host cell cycling
    Hiroki Iwai
    Department of Microbiology and Immunology, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cell 130:611-23. 2007
    ..These results strongly indicate that Shigella employ special tactics to influence epithelial renewal in order to promote bacterial colonization of intestinal epithelium...
  86. ncbi Identification of MAL2, a novel member of the mal proteolipid family, though interactions with TPD52-like proteins in the yeast two-hybrid system
    S H Wilson
    Molecular Oncology Laboratory, Oncology Research Unit, The Children s Hospital at Westmead, Locked Bag 4001, Westmead, NSW, 2145, Australia
    Genomics 76:81-8. 2001
    ..Our study has therefore identified a novel member of the MAL proteolipid family and potentially implicates TPD52-like proteins in vesicle transport...
  87. ncbi Expression of multiple proteins within single primary cortical neurons using a replication deficient HSV vector
    R Coopersmith
    Harvard Medical School, MA, USA
    Biotechniques 27:1156-60. 1999
    ..We conclude that co-infection with HSV vectors is an efficient way to obtain expression of multiple gene products within individual primary culture neurons...
  88. ncbi Fibroblast growth factor-1 interacts with the glucose-regulated protein GRP75/mortalin
    E Mizukoshi
    Biosignaling Department, National Institute of Bioscience and Human Technology, Higashi 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Biochem J 343:461-6. 1999
    ..These results reveal that GRP75/mortalin is an intracellular FGF-1-binding protein in cells and suggest that GRP75/mortalin is involved in the trafficking of and/or signalling by FGF-1...
  89. ncbi The amino-terminal TPR domain of Dia2 tethers SCF(Dia2) to the replisome progression complex
    Hiroko Morohashi
    Cancer Research UK Paterson Institute for Cancer Research, University of Manchester, UK
    Curr Biol 19:1943-9. 2009
    ..Our findings suggest that the amino-terminal domains of other F box proteins might also play an analogous regulatory role, controlling the localization of the cognate SCF complexes...
  90. ncbi FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2
    L Van Den Berghe
    Institut Louis Bugnard, Toulouse, France
    Mol Endocrinol 14:1709-24. 2000
    ..Thus we discuss the possibility that these activities could reflect a physiological function of FIF through its interaction with FGF-2...
  91. ncbi Identification of PamA as a PII-binding membrane protein important in nitrogen-related and sugar-catabolic gene expression in Synechocystis sp. PCC 6803
    Takashi Osanai
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
    J Biol Chem 280:34684-90. 2005
    ..In addition, the pamA mutant was found to be unable to grow in glucose-containing media. These results indicate that PamA has a role in the transcript control of genes for nitrogen and sugar metabolism in Synechocystis sp. PCC 6803...
  92. ncbi Fas-associated factor 1 is a negative regulator of PYRIN-containing Apaf-1-like protein 1
    Takeshi Kinoshita
    Division of Immunology and Molecular Biology, Cancer Research Institute, Kanazawa University, 13 1 Takaramachi, Kanazawa, Ishikawa 920 0934, Japan
    Int Immunol 18:1701-6. 2006
    ..In THP-1 cells, the stimulation of NF-kappaB up-regulated the level of endogenous FAF1. Taken together, these findings suggest that FAF1 functions as a negative regulator of an NF-kappaB signal pathway that involves PYPAF1 and ASC...
  93. ncbi An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair
    G Sebastiaan Winkler
    Department of Physiological Chemistry, University Medical Centre Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    J Mol Biol 337:157-65. 2004
    ..These are the first examples of altered-specificity E2-E3 enzyme pairs and give further insight into how E2-E3 specificity is obtained...
  94. ncbi WormBase: a comprehensive data resource for Caenorhabditis biology and genomics
    Nansheng Chen
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nucleic Acids Res 33:D383-9. 2005
    ..Internally, we have restructured the database models to rationalize the representation of genes and to prepare the system to accept the genome sequences of three additional Caenorhabditis species over the coming year...
  95. ncbi Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation
    Mette Praetorius-Ibba
    Department of Microbiology, The Ohio State University, Columbus, Ohio 43210 1292, USA
    J Biol Chem 280:26099-104. 2005
    ..These findings indicate that ProRS and LeuRS associate in M. thermautotrophicus and suggest that this interaction contributes to translational fidelity by enhancing tRNA aminoacylation by ProRS...
  96. ncbi Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex
    Maximiliano Juri Ayub
    , , , Universidad de Buenos Aires, Argentina
    Gene 357:129-36. 2005
    ..A quantitative evaluation of the interactions established that the strongest interacting pair was TcP0-TcP1beta...
  97. ncbi Linkage map of protein-protein interactions of Porcine teschovirus
    Roland Zell
    Institute for Virology and Antiviral Therapy, Hans Knöll Str 2, D 07745 Jena, Germany
    J Gen Virol 86:2763-8. 2005
    ..Unlike the poliovirus 3C, the teschovirus 3C proteinase dimerizes and interacts with 2BC, 3CD and 3D. The strongest interactions were observed for L-3C, L-3CD and 3C-3CD...
  98. ncbi A role for the RabA4b effector protein PI-4Kbeta1 in polarized expansion of root hair cells in Arabidopsis thaliana
    Mary L Preuss
    Donald Danforth Plant Science Center, St Louis, MO 63132, USA
    J Cell Biol 172:991-8. 2006
    ....
  99. ncbi Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins
    Trond Lamark
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 278:34568-81. 2003
    ..An interaction between the cell polarity scaffold protein Par6 and MEK5 was found. Furthermore, p62 interacts both with MEK5 and NBR1 in addition to the aPKCs. Evidence for involvement of p62 in MEK5-ERK5 signaling is presented...
  100. ncbi Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes
    Hsiao Huei Chen
    Cardiovascular Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 279:30800-6. 2004
    ..Nuclear export of Vgl-4 is dependent on the nuclear exportin CRM-1. These results suggest that Vgl-4 modulates the activity of TEF-1 factors and counteracts alpha1-adrenergic activation of gene expression in cardiac myocytes...
  101. ncbi HBP1 and Mad1 repressors bind the Sin3 corepressor PAH2 domain with opposite helical orientations
    Kurt A Swanson
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Nat Struct Mol Biol 11:738-46. 2004
    ..These results not only provide insights into how multiple, unrelated transcription factors recruit the same coregulator, but also have implications for how sequence similarity searches are conducted...

Research Grants74

  1. Mechanisms of HIV-1 Variation
    LOUIS MANSKY; Fiscal Year: 2003
    ..Finally, cell culture experiments will be done to test mutations in either Vpr or reverse transcriptase that can alter the in vivo fidelity of reverse transcription. ..
  2. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2005
    ....
  3. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2007
    ..abstract_text> ..
  4. INTESTINAL AND PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 1999
    ....
  5. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2003
    ..Furthermore, since homologues of N. crassa G-alpha genes are required for virulence in numerous fungal species, these investigations will also lead to new therapies for emerging fungal pathogens. ..
  6. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2006
    ..Furthermore, since homologues of N. crassa G-alpha genes are required for virulence in numerous fungal species, these investigations will also lead to new therapies for emerging fungal pathogens. ..
  7. PROTEINS INVOLVED IN TRANSPORT THROUGH PLASMODESMATA
    Vitaly Citovsky; Fiscal Year: 2000
    ..The intercellular localization of the MP kinase will be studied in detail, its expression patterns will be examined during plant development, and the plant gene coding for this enzyme will be cloned. ..
  8. Functional Analysis of an hnRNP C-Like Protein, p542
    JAMES MCAFEE; Fiscal Year: 2004
    ..The in-vitro telomere binding specificity of p542, parallels inferences linking hnRNP C to telomeres, and prompts our investigations into the in vivo association of p542 with telomeric DNA. ..
  9. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol S Lim; Fiscal Year: 2010
    ..Our long-term, ultimate goal is to use localization controllable versions of Bcr-Abl (as gene therapy) for treatment of CML. ..
  10. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol Lim; Fiscal Year: 2009
    ..Our long-term, ultimate goal is to use localization controllable versions of Bcr-Abl (as gene therapy) for treatment of CML. ..
  11. A systems level analysis of spore coat assembly in Bacillus subtilis
    Patrick Eichenberger; Fiscal Year: 2010
    ..We anticipate that our results will have an impact on the design of therapeutic approaches to detect and eliminate pathogenic spore-forming bacteria. ..
  12. MOLECULAR BASIS OF IMMUNOGLOBULIN HEAVY CHAIN SWITCH
    Janet Stavnezer; Fiscal Year: 2004
    ..In addition, mice with deficiencies in proteins already known to interact with MMR proteins, but to remain viable, will be tested for their ability to undergo CSR. ..
  13. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan Golden; Fiscal Year: 2004
    ....
  14. The Role of RECQL4 in Bone Development and Osteosarcoma
    Lisa L Wang; Fiscal Year: 2010
    ..This will allow us to discover novel ways to improve the treatment of osteosarcoma and osteoporosis in the general population. ..
  15. Mechanisms of HIV-1 Variation
    LOUIS MANSKY; Fiscal Year: 2006
    ..Finally, cell culture experiments will be done to test mutations in either Vpr or reverse transcriptase that can alter the in vivo fidelity of reverse transcription. ..
  16. DEVELOPMENT OF MEMBRANE GLYCOPROTEIN STRUCTURE
    Philip Thomas; Fiscal Year: 1999
    ..The proposed studies are both necessary and fundamental to understanding the development of membrane protein structure, and may provide novel information relevant to several human diseases. ..
  17. REGULATION OF MALTOSE FERMENTATION IN SACCHAROMYCES
    CORINNE MICHELS; Fiscal Year: 1999
    ..Mutations which broaden inducer specificity will be isolated. The alpha-methylglucoside-activator gene will be isolated and used to characterize the inducer sensing- signaling pathway. ..
  18. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2006
    ..abstract_text> ..
  19. Functional analysis of Helicobacter pylori VacA toxin
    Victor Torres; Fiscal Year: 2003
    ..To fulfill this aim I am using the VacA recombinant system to determine the minimum amino acid sequence required for the delta6-27-VacA toxin to exhibit a dominant-negative phenotype. ..
  20. HYPOHIDROTIC ECTODERMAL DYSPLASIA--A GENETIC ANALYSIS
    Jonathan Zonana; Fiscal Year: 2002
    ..At the same time, the new knowledge should rapidly result in improved genetic diagnosis and counseling, and may even suggest avenues for future therapies. ..
  21. DIMERIZATION & COOPERATIVITY IN PROKARYOTIC GENE CONTROL
    Ann Hochschild; Fiscal Year: 2002
    ..This assay will also be applied to the study and manipulation of a phosphorylation-dependent protein-protein interaction involving the eukaryotic transcription factor CREB and its CREB and its co-activator CBP. ..
  22. Roles of Lig3 and XRCC1 Genes in Genome Stability
    Alan E Tomkinson; Fiscal Year: 2010
    ..These differences between normal and cancer cells offer an opportunity to develop therapeutic strategies that selectively target cancer cells. ..
  23. Characterization of Chlamydial Inclusion Migration
    Scott Grieshaber; Fiscal Year: 2007
    ....
  24. Signaling Mechanisms Behind nAChR Clustering at the NMJ
    Jenny Linnoila; Fiscal Year: 2007
    ..Thus, studying the fundamental process of neuromuscular development is critical to understanding the causes of and to designing more effective treatments for these debilitating conditions. ..