two hybrid system techniques

Summary

Summary: Screening techniques used to identify genes encoding interacting proteins. Variations are used to evaluate complex interplay between proteins and other molecules.

Top Publications

  1. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
  2. pmc Evaluation of clustering algorithms for protein-protein interaction networks
    Sylvain Brohée
    Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
    BMC Bioinformatics 7:488. 2006
  3. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
  4. pmc High-quality binary protein interaction map of the yeast interactome network
    Haiyuan Yu
    Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 322:104-10. 2008
  5. doi TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis
    Heidi Szemenyei
    Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 319:1384-6. 2008
  6. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
  7. pmc HIV-1 Vpr-mediated G2 arrest involves the DDB1-CUL4AVPRBP E3 ubiquitin ligase
    Jean Philippe Belzile
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada
    PLoS Pathog 3:e85. 2007
  8. ncbi SoxE factors function equivalently during neural crest and inner ear development and their activity is regulated by SUMOylation
    Kimberly M Taylor
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Dev Cell 9:593-603. 2005
  9. pmc Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK
    Chie Kanei-Ishii
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305 0074, Japan
    Genes Dev 18:816-29. 2004
  10. ncbi Sensing of Lys 63-linked polyubiquitination by NEMO is a key event in NF-kappaB activation [corrected]
    Chuan Jin Wu
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 8:398-406. 2006

Research Grants

  1. Mechanisms of HIV-1 Variation
    LOUIS MANSKY; Fiscal Year: 2003
  2. SGK Regulation of Epithelial Sodium Transport
    David Pearce; Fiscal Year: 2011
  3. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2000
  4. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2002
  5. MECHANISMS OF YEAST TRANSCRIPTIONAL INITITATION
    Kevin Struhl; Fiscal Year: 1993
  6. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2001
  7. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 1999
  8. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2005
  9. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2004
  10. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2001

Detail Information

Publications280 found, 100 shown here

  1. ncbi Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
    P Beguin
    Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Nature 411:701-6. 2001
    ..We propose that the small G-protein kir/Gem, interacting with beta-subunits, regulates Ca2+ channel expression at the cell surface...
  2. pmc Evaluation of clustering algorithms for protein-protein interaction networks
    Sylvain Brohée
    Service de Conformation des Macromolécules Biologiques et de Bioinformatique, Universite Libre de Bruxelles, CP 263, Campus Plaine, Bd, du Triomphe, B 1050 Bruxelles, Belgium
    BMC Bioinformatics 7:488. 2006
    ..In this paper we present a comparative assessment of four algorithms: Markov Clustering (MCL), Restricted Neighborhood Search Clustering (RNSC), Super Paramagnetic Clustering (SPC), and Molecular Complex Detection (MCODE)...
  3. ncbi Specificity and stability in topology of protein networks
    Sergei Maslov
    Department of Physics, Brookhaven National Laboratory, Upton, NY 11973, USA
    Science 296:910-3. 2002
    ..This effect decreases the likelihood of cross talk between different functional modules of the cell and increases the overall robustness of a network by localizing effects of deleterious perturbations...
  4. pmc High-quality binary protein interaction map of the yeast interactome network
    Haiyuan Yu
    Center for Cancer Systems Biology CCSB, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 322:104-10. 2008
    ..Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy...
  5. doi TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis
    Heidi Szemenyei
    Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 319:1384-6. 2008
    ..These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development...
  6. ncbi Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
    J E Garrus
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Cell 107:55-65. 2001
    ..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies...
  7. pmc HIV-1 Vpr-mediated G2 arrest involves the DDB1-CUL4AVPRBP E3 ubiquitin ligase
    Jean Philippe Belzile
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada
    PLoS Pathog 3:e85. 2007
    ....
  8. ncbi SoxE factors function equivalently during neural crest and inner ear development and their activity is regulated by SUMOylation
    Kimberly M Taylor
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Dev Cell 9:593-603. 2005
    ..These findings provide important mechanistic insight into how the activity of widely deployed developmental regulatory proteins can be directed to specific developmental events...
  9. pmc Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK
    Chie Kanei-Ishii
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305 0074, Japan
    Genes Dev 18:816-29. 2004
    ..The down-regulation of Myb by Wnt-1 signal may play an important role in a variety of developmental steps...
  10. ncbi Sensing of Lys 63-linked polyubiquitination by NEMO is a key event in NF-kappaB activation [corrected]
    Chuan Jin Wu
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 8:398-406. 2006
    ..These results provide a mechanism for NEMO's critical role in IKK activation, and a key to understanding the link between cytokine-receptor proximal signalling and IKK and NF-kappaB activation...
  11. ncbi PHD domain-mediated E3 ligase activity directs intramolecular sumoylation of an adjacent bromodomain required for gene silencing
    Alexey V Ivanov
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell 28:823-37. 2007
    ..These data provide a mechanistic explanation for the cooperation of PHD and bromodomains in gene regulation and describe a function of the PHD domain as an intramolecular E3 SUMO ligase...
  12. pmc Group 13 HOX proteins interact with the MH2 domain of R-Smads and modulate Smad transcriptional activation functions independent of HOX DNA-binding capability
    Thomas M Williams
    Department of Human Genetics, University of Michigan Ann Arbor, MI, USA
    Nucleic Acids Res 33:4475-84. 2005
    ....
  13. ncbi The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif
    K W Lo
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People s Republic of China
    J Biol Chem 276:14059-66. 2001
    ..The role of each conserved amino acid residue in this pentapeptide motif in supporting complex formation with DLC8 was systematically studied using site-directed mutagenesis...
  14. pmc ZNF265--a novel spliceosomal protein able to induce alternative splicing
    D J Adams
    The University of Sydney, Basic and Clinical Genomics Laboratory, Department of Physiology and Institute for Biomedical Research, Sydney, NSW 2006, Australia
    J Cell Biol 154:25-32. 2001
    ..Alignment with other RS domain-containing proteins revealed a high degree of SR dipeptide conservation. These data show that ZNF265 functions as a novel component of the mRNA processing machinery...
  15. ncbi Interaction of c-Jun amino-terminal kinase interacting protein-1 with p190 rhoGEF and its localization in differentiated neurons
    D Meyer
    Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Biol Chem 274:35113-8. 1999
    ..The interaction of JIP-1 with rhoGEF and its subcellular localization suggests that JIP-1 may function to specifically localize a signaling complex in neuronal cells...
  16. ncbi Galpha13 stimulates cell migration through cortactin-interacting protein Hax-1
    V Radhika
    Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 279:49406-13. 2004
    ..These findings, along with the observation that Hax-1 is overexpressed in metastatic tumors and tumor cell lines, suggest a novel role for the association of oncogenic Galpha13 and Hax-1 in tumor metastasis...
  17. ncbi The interaction of plectin with actin: evidence for cross-linking of actin filaments by dimerization of the actin-binding domain of plectin
    L Fontao
    The Netherlands Cancer Institute, Division of Cell Biology, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Cell Sci 114:2065-76. 2001
    ..We propose a new mechanism by which plectin and possibly also other actin-binding proteins can regulate the organization of the F-actin network in the cell...
  18. pmc The tripartite motif family identifies cell compartments
    A Reymond
    Telethon Institute of Genetics and Medicine TIGEM, San Raffaele Biomedical Science Park, Milan, Italy
    EMBO J 20:2140-51. 2001
    ..A total of 37 TRIM genes/proteins were studied, 21 of which were novel. The results demonstrate that TRIM proteins share a common function: by means of homo-multimerization they identify specific cell compartments...
  19. ncbi HOXA5-twist interaction alters p53 homeostasis in breast cancer cells
    Ioannis A Stasinopoulos
    Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 280:2294-9. 2005
    ..Thus, the loss of HOXA5 expression could lead to the functional activation of Twist resulting in aberrant cell cycle regulation and promoting breast carcinogenesis...
  20. ncbi Matrix and Steiner-triple-system smart pooling assays for high-performance transcription regulatory network mapping
    Vanessa Vermeirssen
    Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Methods 4:659-64. 2007
    ..Both strategies outperform library screens in terms of coverage, confidence and throughput. These versatile strategies can be adapted both to TFs in other systems and, likely, to other biomolecules and assays as well...
  21. ncbi Specific interaction of the potassium channel beta-subunit minK with the sarcomeric protein T-cap suggests a T-tubule-myofibril linking system
    T Furukawa
    Department of Physiology, Akita University School of Medicine, Japan
    J Mol Biol 313:775-84. 2001
    ..We speculate that this interaction may contribute to a stretch-dependent regulation of potassium flux in cardiac muscle, providing a "mechano-electrical feedback" system...
  22. ncbi Voltage-dependent anion-selective channel (VDAC) interacts with the dynein light chain Tctex1 and the heat-shock protein PBP74
    Christian Schwarzer
    Max Planck Institute for Experimental Medicine, Department of Immunochemistry, Hermann Rein Street 3, 37075 Gottingen, Germany
    Int J Biochem Cell Biol 34:1059-70. 2002
    ....
  23. ncbi Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB
    Simona Masiero
    Dipartimento di Genetica e Biologia dei Microrganismi, Universita di Milano, Via Celoria 26, 20133 Milano, Italy
    J Biol Chem 277:26429-35. 2002
    ..This is the first indication that members of the NF-YB family work through mechanisms independent of the CCAAT box...
  24. pmc Pim serine/threonine kinases regulate the stability of Socs-1 protein
    X Peter Chen
    Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 99:2175-80. 2002
    ..These data suggest that the Pim kinases may regulate cytokine-induced JAK-STAT signaling through modulation of Socs-1 protein levels...
  25. ncbi Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae
    J Thomas Hannich
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:4102-10. 2005
    ..The results point to a surprisingly broad array of cellular processes regulated by SUMO conjugation and provide a starting point for detailed studies of how SUMO ligation contributes to these different regulatory mechanisms...
  26. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  27. pmc The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity
    J E Adamo
    Department of Cell Biology, Cell Biology, and Genetics, Weill Medical College of Cornell University, New York, New York 10021, USA
    Mol Biol Cell 10:4121-33. 1999
    ..These data suggest that Rho3 acts as a key regulator of cell polarity and exocytosis, coordinating several distinct events for delivery of proteins to specific sites on the cell surface...
  28. ncbi A protein interaction network of the malaria parasite Plasmodium falciparum
    Douglas J LaCount
    Howard Hughes Medical Institute, University of Washington, Box 357730, Seattle, Washington 98195, USA
    Nature 438:103-7. 2005
    ..These data constitute the first extensive description of the protein interaction network for this important human pathogen...
  29. pmc The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex
    Beatriz Aranda-Orgilles
    Max Planck Institute for Molecular Genetics, Ihnestr 73, 14195, Berlin, Germany
    Hum Genet 123:163-76. 2008
    ..The dysfunction of this mechanism would lead to malfunction of microtubule-associated protein translation and to the development of OS...
  30. ncbi Subcellular targeting of metabolic enzymes to titin in heart muscle may be mediated by DRAL/FHL-2
    Stephan Lange
    Institute of Cell Biology, ETH Honggerberg, 8093 Zurich, Switzerland
    J Cell Sci 115:4925-36. 2002
    ..We propose that DRAL/FHL-2 acts as a specific adaptor protein to couple metabolic enzymes to sites of high energy consumption in the cardiac sarcomere...
  31. pmc Human Vam6p promotes lysosome clustering and fusion in vivo
    S Caplan
    Cell Biology and Metabolism Branch at the National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 154:109-22. 2001
    ..This study implicates hVam6p as a mammalian tethering/docking factor characterized with intrinsic ability to promote lysosome fusion in vivo...
  32. ncbi Negative regulation of the mammalian UV response by Myc through association with Miz-1
    Steffi Herold
    Institute for Molecular Biology and Tumor Research, 35033 Marburg, Germany
    Mol Cell 10:509-21. 2002
    ..Our data implicate Miz-1 in a pathway that regulates cell proliferation in response to UV irradiation...
  33. ncbi Annexin II light chain regulates sensory neuron-specific sodium channel expression
    Kenji Okuse
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Nature 417:653-6. 2002
    ..Because direct association with p11 is required for functional expression of Na(V)1.8, disrupting this interaction may be a useful new approach to downregulating Na(V)1.8 and effecting analgesia...
  34. pmc Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex
    Leila Alland
    Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 22:2743-50. 2002
    ....
  35. ncbi Mammalian 14-3-3beta associates with the Chlamydia trachomatis inclusion membrane via its interaction with IncG
    M A Scidmore
    Host Parasite Interactions Section, Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Mol Microbiol 39:1638-50. 2001
    ..psittaci or C. pneumoniae inclusions. 14-3-3beta is the first eukaryotic protein found to interact with the chlamydial inclusion; however, its unique role in C. trachomatis pathogenesis remains to be determined...
  36. ncbi Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain
    T Centner
    European Molecular Biology Laboratory, Heidelberg, D 69117, Germany
    J Mol Biol 306:717-26. 2001
    ....
  37. pmc Abscisic acid signaling in seeds and seedlings
    Ruth R Finkelstein
    Department of Molecular, Cellular, and Developmental Biology, University of California at Santa Barbara, Santa Barbara, CA 93106, USA
    Plant Cell 14:S15-45. 2002
  38. ncbi FANCC, FANCE, and FANCD2 form a ternary complex essential to the integrity of the Fanconi anemia DNA damage response pathway
    Susan M Gordon
    Program in Genetics and Genomic Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 280:36118-25. 2005
    ..Thus, FANCE is shown to be a key mediator of protein interactions both in the architecture of the FA protein complex and in the connection of complex components to the putative downstream targets of complex activity...
  39. ncbi Gib2, a novel Gbeta-like/RACK1 homolog, functions as a Gbeta subunit in cAMP signaling and is essential in Cryptococcus neoformans
    Daniel A Palmer
    Research Institute for Children, Department of Pediatrics, Louisiana State University Health Sciences Center, 200 Henry Clay Avenue, New Orleans, LA 70118, USA
    J Biol Chem 281:32596-605. 2006
    ..Finally, Gib2 is shown to also physically interact with a downstream target of Gpa1-cAMP signaling, Smg1, and the protein kinase C homolog Pkc1, indicating that Gib2 is also a multifunctional RACK1-like protein...
  40. ncbi E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages
    Devin E Christensen
    Department of Biochemistry, University of Washington, Box 357350, Seattle, Washington 98195 7350, USA
    Nat Struct Mol Biol 14:941-8. 2007
    ..Thus, BRCA1 has the ability to direct the synthesis of specific polyubiquitin chain linkages, depending on the E2 bound to its RING...
  41. pmc The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases
    V Albrecht
    Universitat Ulm, Molekulare Botanik, Albert Einstein Allee 11, D 89069 Ulm, Germany
    EMBO J 20:1051-63. 2001
    ....
  42. ncbi The nuclear accumulation of the Fanconi anemia protein FANCE depends on FANCC
    France Léveillé
    Department of Clinical Genetics and Human Genetics, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    DNA Repair (Amst) 5:556-65. 2006
    ..This strengthens the idea that FANCE recruits FANCD2 to the core complex, without interfering with the binding of FANCC...
  43. pmc Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules
    Takeshi Nakamura
    Department of Molecular Genetics, National Institute for Longevity Sciences, Program of Protecting the Brain, CREST, JST, Oobu, Aichi 474 8522, Japan
    Mol Cell Biol 22:8721-34. 2002
    ..These results suggest that Grit, a novel TrkA-interacting protein, regulates neurite outgrowth by modulating the Rho family of small GTPases...
  44. ncbi TGF-beta-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation
    R Perlman
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Cell Biol 3:708-14. 2001
    ..Our findings associate Daxx directly with the TGF-beta apoptotic-signalling pathway, and make a biochemical connection between the receptors for TGF-beta and the apoptotic machinery...
  45. ncbi TrioGEF1 controls Rac- and Cdc42-dependent cell structures through the direct activation of rhoG
    A Blangy
    Centre de Recherche en Biochimie Macromoleculaire, CRBM CNRS UPR1086, IFR24, 34293 Montpellier Cedex 5, France
    J Cell Sci 113:729-39. 2000
    ..These data demonstrate that Trio controls a signaling cascade that activates RhoG, which in turn activates Rac1 and Cdc42Hs...
  46. ncbi Myosin V orientates the mitotic spindle in yeast
    H Yin
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nature 406:1013-5. 2000
    ..The tail domain of Myo2 that binds Kar9 also interacts with secretory vesicles and vacuolar elements, making it a pivotal component of yeast cell polarization...
  47. ncbi A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae
    S L Ooi
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Science 294:2552-6. 2001
    ..Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer...
  48. pmc Enhanceosome formation over the beta interferon promoter underlies a remote-control mechanism mediated by YY1 and YY2
    Martin Klar
    German Research Center for Biotechnology GBF, RDIF Epigenetic Regulation, Mascheroder Weg 1, 38124 Braunschweig, Germany
    Mol Cell Biol 25:10159-70. 2005
    ..Differences between the human and murine control regions will be described...
  49. pmc Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins
    Katsumi Kasashima
    Department of Biology, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    Nucleic Acids Res 30:4519-26. 2002
    ..These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs...
  50. ncbi A pathway for association of receptors, adaptors, and actin during endocytic internalization
    Marko Kaksonen
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 115:475-87. 2003
    ..Attention is now focused on the mechanisms by which the order and timing of events in this endocytic pathway are achieved...
  51. doi The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein
    Isabelle Imbert
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, Marseille Cedex 9, France
    Virus Res 133:136-48. 2008
    ..The interaction map thus provides a sum of the interactions that may occur at some point during coronavirus RNA synthesis and provides a framework for future research...
  52. pmc Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein
    K Yeung
    Department of Molecular Biology, Brown University, Providence, Rhode Island 02912, USA
    Mol Cell Biol 20:3079-85. 2000
    ..The properties of RKIP reveal the specific sequestration of interacting components as a novel motif in the cell's repertoire for the regulation of signaling pathways...
  53. ncbi Myotilin is mutated in limb girdle muscular dystrophy 1A
    M A Hauser
    Department of Medicine, Section of Medical Genetics, Duke University Medical Center, DUMC 3445, Durham, NC 27710 3445, USA
    Hum Mol Genet 9:2141-7. 2000
    ..Myotilin is a sarcomeric protein that binds to alpha-actinin and is localized in the Z-line. The observed missense mutation does not disrupt binding to alpha-actinin...
  54. pmc Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome
    Joy Armistead
    Department of Biochemistry and Medical Genetics, University of Manitoba, MB, Canada
    Am J Hum Genet 84:728-39. 2009
    ..These findings suggested that the D-to-G mutation caused aggregation of EMG1, thereby reducing the level of the protein and causing BCS...
  55. ncbi Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1
    Erwann Le Rouzic
    Institut Cochin, Department of Infectious Diseases, CNRS UMR8104, INSERM U567, Universite Paris 5, Paris, France
    J Biol Chem 277:45091-8. 2002
    ..This association of Vpr with a component of the nuclear pore complex may contribute to the disruption of the nuclear envelope and to the nuclear import of the viral DNA...
  56. ncbi A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
    X Cao
    The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
    Science 293:115-20. 2001
    ..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
  57. pmc Functional and physical interactions between components of the Prp19p-associated complex
    Chun Hong Chen
    Institute of Molecular Biology, Academia Sinica, Nankang, Taiwan, Republic of China
    Nucleic Acids Res 30:1029-37. 2002
    ..Genetic analysis suggests that Ntc31p, Ntc30p and Ntc20p may play roles in modulating the function of Ntc90p...
  58. ncbi MAPPIT: a cytokine receptor-based two-hybrid method in mammalian cells
    J Tavernier
    VIB09 Department of Medical Protein Research, Faculty of Medicine and Health Sciences, Department of Biochemistry, Ghent University, Ghent Belgium
    Clin Exp Allergy 32:1397-404. 2002
    ....
  59. pmc Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioning
    Erik D Andrulis
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794 5215, USA
    Mol Cell Biol 22:8292-301. 2002
    ..Our data suggest that Esc1 is a component of a redundant pathway that functions to localize silencing complexes to the nuclear periphery...
  60. pmc Conserved patterns of protein interaction in multiple species
    Roded Sharan
    Computer Science Division, University of California, Berkeley, CA 94704, USA
    Proc Natl Acad Sci U S A 102:1974-9. 2005
    ....
  61. ncbi A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 125:801-14. 2006
    ..This interactome thus provides a tool for understanding pathogenic mechanisms common for this class of neurodegenerative disorders and for identifying candidate genes for inherited ataxias...
  62. pmc TACC3 mediates the association of MBD2 with histone acetyltransferases and relieves transcriptional repression of methylated promoters
    Tiziana Angrisano
    Dipartimento di Biologia e Patologia Cellulare e Molecolare L Califano, Universita degli Studi di Napoli Federico II, 80131 Naples, Italy
    Nucleic Acids Res 34:364-72. 2006
    ....
  63. pmc Lipoprotein receptors and a disabled family cytoplasmic adaptor protein regulate EGL-17/FGF export in C. elegans
    Darren M Kamikura
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 17:2798-811. 2003
    ..These results suggest a model in which Ce-DAB-1 coordinates selection of receptors and cargo, including EGL-17, for transport through the secretory pathway...
  64. ncbi Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex
    Seiji Matsumoto
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 280:42536-42. 2005
    ..These data suggest that Hsk1-Dfp1/Him1 and Swi1-Swi3 complexes have interrelated roles in stabilization of arrested replication forks...
  65. ncbi Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1
    Chengtao Her
    School of Molecular Biosciences, Center for Reproductive Biology, P O Box 644660, Washington State University, Pullman, WA 99164 4660, USA
    DNA Repair (Amst) 1:719-29. 2002
    ..Together, these data suggest that hMRE11 and hMLH1 might act in a co-operative fashion during DNA damage detection, signaling, and repair...
  66. pmc Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein
    X R Ren
    Department of Pathology and Cell Adhesion and Matrix Center, Pathology, and Physical Medicine and Rehabilitation, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Cell Biol 152:971-84. 2001
    ..Taken together, our results suggest that PSGAP is a signaling protein essential for PYK2 regulation of cytoskeletal organization via Rho family GTPases...
  67. ncbi Multiple Skp1-related proteins in Caenorhabditis elegans: diverse patterns of interaction with Cullins and F-box proteins
    Atsushi Yamanaka
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, 812 8582, Fukuoka, Japan
    Curr Biol 12:267-75. 2002
    ..The biochemical properties, expression, and function of the C. elegans SKR proteins were examined...
  68. pmc The formin-homology protein SmDia interacts with the Src kinase SmTK and the GTPase SmRho1 in the gonads of Schistosoma mansoni
    Thomas Quack
    Institute for Parasitology, Justus Liebig University, Giessen, Germany
    PLoS ONE 4:e6998. 2009
    ..Since Src kinases are important signal transduction proteins it is of interest to unravel the signaling cascades SmTK3 is involved in to understand its cellular role in the gonads...
  69. ncbi Herpesviral protein networks and their interaction with the human proteome
    Peter Uetz
    Institut fur Genetik, Forschungszentrum Karlsruhe, Postfach 3640, Karlsruhe, D 76021 Germany
    Science 311:239-42. 2006
    ..The analysis of the combined system showed that the viral network adopts cellular network features and that protein networks of herpesviruses and possibly other intracellular pathogens have distinguishing topologies...
  70. ncbi The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2
    C Wagner
    Institut fur Mikrobiologie, Abteilung Genetik und Biochemie, Ernst Moritz Arndt Universitat Greifswald, Jahnstr 15a, D 17487 Greifswald, Germany
    Mol Microbiol 41:155-66. 2001
    ..These results define the molecular composition of the transcription complex mediating control of ICRE-dependent genes and allow a hypothesis on the flow of regulatory information in response to phospholipid precursors...
  71. pmc Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast
    Andre P Gerber
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E79. 2004
    ....
  72. ncbi The FANCG Fanconi anemia protein interacts with CYP2E1: possible role in protection against oxidative DNA damage
    Makoto Futaki
    Hematology Branch, NHLBI, NIH, Building 10, Room 7C103, Bethesda, MD 20892, USA
    Carcinogenesis 23:67-72. 2002
    ..We conclude that the unknown function of FANCG involves at least transient interaction with cytoplasmic components, possibly including CYP2E1, and propose a role for FANCG in protection against oxidative DNA damage...
  73. ncbi The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting
    Keiichi Katoh
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    J Biol Chem 278:39104-13. 2003
    ..These results suggest that CHMP4b and Alix participate in formation of multivesicular bodies by cooperating with SKD1...
  74. pmc NDR1 interaction with RIN4 mediates the differential activation of multiple disease resistance pathways in Arabidopsis
    Brad Day
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94270, USA
    Plant Cell 18:2782-91. 2006
    ..We suggest that this interaction serves to further regulate activation of disease resistance signaling following recognition of P. syringae DC3000-AvrRpt2 by Arabidopsis...
  75. ncbi A root-knot nematode secretory peptide functions as a ligand for a plant transcription factor
    Guozhong Huang
    Department of Plant Pathology, University of Georgia, Athens 30602 7274, USA
    Mol Plant Microbe Interact 19:463-70. 2006
    ..This discovery is the first demonstration of a direct interaction of a nematode-secreted parasitism peptide with a plant-regulatory protein, which may represent an early signaling event in the root-knot nematode-host interaction...
  76. pmc The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif
    Sophie Deltour
    CNRS UMR 8526, Institut de Biologie de Lille, Institut Pasteur de Lille, 59017 Lille Cedex, France
    Mol Cell Biol 22:4890-901. 2002
    ..In conclusion, our results demonstrate that HIC1 mediates transcriptional repression by both HDAC-independent and HDAC-dependent mechanisms and show that CtBP is a HIC1 corepressor that is recruited via a variant binding site...
  77. ncbi Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors
    A Kiss
    Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
    Virology 314:591-600. 2003
    ..These results suggest a physiological role for hNup98 in modulating Rev-dependent RNA export during HIV infection...
  78. pmc A large-scale protein protein interaction analysis in Synechocystis sp. PCC6803
    Shusei Sato
    Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
    DNA Res 14:207-16. 2007
    ..The interaction data obtained in this study should provide new insights and novel strategies for functional analyses of genes in Synechocystis, and, additionally, genes in other cyanobacteria and plant genes of cyanobacterial origin...
  79. pmc Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases
    Hitoshi Yoshida
    Department of Rice Research, National Agricultural Research Center, Jo etsu, Niigata 943 0193, Japan
    BMC Plant Biol 5:14. 2005
    ..Because orthologous sequences to ETO1 are found in many plant species including tomato, we transformed tomato with Arabidopsis ETO1 to evaluate its ability to suppress ethylene production in tomato fruits...
  80. doi Biochemistry. Not comparable, but complementary
    Lars Juhl Jensen
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Science 322:56-7. 2008
  81. ncbi LeSTIG1, an extracellular binding partner for the pollen receptor kinases LePRK1 and LePRK2, promotes pollen tube growth in vitro
    Weihua Tang
    Plant Gene Expression Center, United States Department of Agriculture Agricultural Research Service, and Department of Plant and Microbial Biology, University of California at Berkeley, 800 Buchanan Street, Albany, CA 94710, USA
    Plant J 39:343-53. 2004
    ..Exogenous LeSTIG1 promotes pollen tube growth in vitro. The interaction of these pollen kinases with LeSTIG1 supports the notion that LePRK1 and LePRK2 are involved in mediating pollen-pistil interactions...
  82. pmc CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription
    Linyu Li
    Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology and School of Medicine, Tsinghua University, Beijing 100084, People s Republic of China
    Mol Cell Biol 24:856-64. 2004
    ..These results suggest that CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins...
  83. doi The YPWM motif links Antennapedia to the basal transcriptional machinery
    Frederic Prince
    Biozentrum, University of Basel, Klingelberstrasse 70, CH 4056 Basel, Switzerland
    Development 135:1669-79. 2008
    ..BIP2 is a TATA-binding protein associated factor (also known as dTAFII3) that links ANTP to the basal transcriptional machinery...
  84. ncbi TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus
    Nicolas Le May
    Unité de Génétique Moléculaire des Bunyaviridés, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Cell 116:541-50. 2004
    ..These observations shed light on the mechanism utilized by RVFV to evade the host response...
  85. pmc A novel motif in geminivirus replication proteins interacts with the plant retinoblastoma-related protein
    Gerardo Arguello-Astorga
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 7622, USA
    J Virol 78:4817-26. 2004
    ..Mutation of the leucine residue in helix 4 of CaLCuV AL1 reduced binding. Together, these results suggest that helix 4 and the conserved leucine residue are part of a pRBR-binding interface in begomovirus replication proteins...
  86. ncbi Direct binding of the N-terminus of HTLV-1 tax oncoprotein to cyclin-dependent kinase 4 is a dominant path to stimulate the kinase activity
    Junan Li
    Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 42:6921-8. 2003
    ..We also report that Tax40N retains the transactivation ability. These results of in vitro studies demonstrate a potentially novel, p16-independent route to regulate CDK4 activity by the Tax oncoprotein in HTLV-1 infected cells...
  87. ncbi SCAMP1 function in endocytosis
    R Fernandez-Chacon
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
    J Biol Chem 275:12752-6. 2000
    ..Our data suggest that one of the functions of SCAMPs is to participate in endocytosis via a mechanism which may involve the recruitment of clathrin coats to the plasma membrane and the trans-Golgi network...
  88. ncbi A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans
    Sarah L Crittenden
    Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 417:660-3. 2002
    ..We suggest that regulation by PUF proteins may be an ancient and widespread mechanism for control of stem cells...
  89. ncbi A Functional interaction between the human papillomavirus 16 transcription/replication factor E2 and the DNA damage response protein TopBP1
    Winifred Boner
    Institute of Comparative Medicine, Department of Veterinary Pathology, University of Glasgow, Garscube Estate, Bearsden Road, Glasgow G61 1QH, Scotland
    J Biol Chem 277:22297-303. 2002
    ..Removal of the amino-terminal domain of TopBP1 abolishes coactivation of transcription and replication. This interaction may have functional consequences upon the viral life cycle...
  90. pmc Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis
    Gouzel Karimova
    Unité de Biochimie des Interactions Macromoléculaires, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Bacteriol 187:2233-43. 2005
    ..In addition, our study shows that the cAMP-based two-hybrid system is particularly appropriate for analyzing molecular interactions between membrane proteins...
  91. pmc NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway
    M Frank-Vaillant
    Laboratoire du Cycle Cellulaire, Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Genes Dev 15:3005-12. 2001
    ..Increasing the dosage of the Lif2p protein can suppress the NHEJ defect in a/alpha cells. Together, these results indicate that NHEJ regulation by mating type is achieved, at least in part, by a regulation of Lif2p activity...
  92. pmc Comprehensive interaction map of the Arabidopsis MADS Box transcription factors
    Stefan de Folter
    Business Unit Bioscience, Plant Research International, 6700 AA Wageningen, The Netherlands
    Plant Cell 17:1424-33. 2005
    ..Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower...
  93. ncbi New insights into the molecular basis of progressive myoclonus epilepsy: a multiprotein complex with cystatin B
    Rossella Di Giaimo
    Department of Biology, Bologna University, 40126 Bologna, Italy
    Hum Mol Genet 11:2941-50. 2002
    ..We propose that a cystatin B multiprotein complex has a specific cerebellar function and that the loss of this function might contribute to the disease in EPM1 patients...
  94. ncbi Inhibition of sodium/proton exchange by a Rab-GTPase-activating protein regulates endosomal traffic in yeast
    Rashid Ali
    Department of Physiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:4498-506. 2004
    ..Our findings provide a possible molecular link between intraendosomal pH and regulation of vesicular trafficking...
  95. ncbi Nine-amino-acid transactivation domain: establishment and prediction utilities
    Simona Piskacek
    Simopharm, Max F Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, A 1030 Vienna, Austria
    Genomics 89:756-68. 2007
    ..A computational search for the 9aa TAD is available online from National EMBnet-Node Austria at http://www.at.embnet.org/toolbox/9aatad/...
  96. ncbi Characterization of a carboxy-terminal BRCA1 interacting protein
    A K Wong
    Myriad Genetics, Inc, Salt Lake City, Utah 84108, USA
    Oncogene 17:2279-85. 1998
    ..Thus it is plausible that CtIP may itself be a tumor suppressor acting in the same pathway as BRCA1...
  97. ncbi A receptor for activated C kinase is part of messenger ribonucleoprotein complexes associated with polyA-mRNAs in neurons
    Frank Angenstein
    Beckman Institute Neuronal Pattern Analysis, University of Illinois, Urbana, Illinois 61801, USA
    J Neurosci 22:8827-37. 2002
    ....
  98. pmc MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana
    Kiyoshi Tatematsu
    Division of Biological Sciences, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Plant Cell 16:379-93. 2004
    ..These results suggest that MSG2/IAA19 and NPH4/ARF7 may constitute a negative feedback loop to regulate differential growth responses of hypocotyls and lateral root formation...
  99. ncbi Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex
    S Krugmann
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, CRC Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Curr Biol 11:1645-55. 2001
    ..Cdc42 controls the formation of actin bundle-containing filopodia at the cellular periphery. The molecular mechanism for this remains as yet unclear...
  100. doi Domain II mutations in CRANE/IAA18 suppress lateral root formation and affect shoot development in Arabidopsis thaliana
    Takeo Uehara
    Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai, Kobe, 657 8501 Japan
    Plant Cell Physiol 49:1025-38. 2008
    ..Taken together, our results indicate that CRANE/IAA18 is involved in lateral root formation in Arabidopsis, and suggest that it negatively regulates the activity of ARF7 and ARF19 for lateral root formation...
  101. ncbi A tctex1-Ca2+ channel complex for selective surface expression of Ca2+ channels in neurons
    Meizan Lai
    Department of Pharmacology, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, Pennsylvania 19104, USA
    Nat Neurosci 8:435-42. 2005
    ..These results underscore the importance of the specific tctex1-channel interaction in determining sorting and trafficking of neuronal Ca(2+) channels with different functionalities...

Research Grants74

  1. Mechanisms of HIV-1 Variation
    LOUIS MANSKY; Fiscal Year: 2003
    ..Finally, cell culture experiments will be done to test mutations in either Vpr or reverse transcriptase that can alter the in vivo fidelity of reverse transcription. ..
  2. SGK Regulation of Epithelial Sodium Transport
    David Pearce; Fiscal Year: 2011
    ....
  3. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2000
    ..Seventh, we will obtain new GCN4 derivatives that have altered DNA-binding specificity and design GCN4 mutants and target sites to test specific hypotheses that arise from the X-ray structure of the protein-DNA complex. ..
  4. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2002
    ..Seventh, we will obtain new GCN4 derivatives that have altered DNA-binding specificity and design GCN4 mutants and target sites to test specific hypotheses that arise from the X-ray structure of the protein-DNA complex. ..
  5. MECHANISMS OF YEAST TRANSCRIPTIONAL INITITATION
    Kevin Struhl; Fiscal Year: 1993
    ..Seventh, we will obtain new GCN4 derivatives that have altered DNA-binding specificity and design GCN4 mutants and target sites to test specific hypotheses that arise from the X-ray structure of the protein-DNA complex...
  6. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 2001
    ..Seventh, we will obtain new GCN4 derivatives that have altered DNA-binding specificity and design GCN4 mutants and target sites to test specific hypotheses that arise from the X-ray structure of the protein-DNA complex. ..
  7. MECHANISMS OF YEAST TRANSCRIPTIONAL INITIATION
    Kevin Struhl; Fiscal Year: 1999
    ..Seventh, we will obtain new GCN4 derivatives that have altered DNA-binding specificity and design GCN4 mutants and target sites to test specific hypotheses that arise from the X-ray structure of the protein-DNA complex. ..
  8. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2005
    ....
  9. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2004
    ....
  10. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2001
    ....
  11. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2003
    ....
  12. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2002
    ....
  13. ROLE OF CALCINEURIN IN C. NEOFORMANS MATING AND FRUITING
    Joseph Heitman; Fiscal Year: 2001
    ....
  14. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2005
    ..abstract_text> ..
  15. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2006
    ..abstract_text> ..
  16. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2004
    ..abstract_text> ..
  17. MOLECULAR BIOLOGY OF BILE ACID SYNTHESIS
    JOHN CHIANG; Fiscal Year: 2007
    ..abstract_text> ..
  18. INTESTINAL & PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 1999
    ..Lastly, the proposed work almost completes the studies that follow directly from the discovery by the P.I. of MHC class I-related FcRs, and additional time will be essential to consider and plan future research directions. ..
  19. INTESTINAL & PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 2000
    ..Lastly, the proposed work almost completes the studies that follow directly from the discovery by the P.I. of MHC class I-related FcRs, and additional time will be essential to consider and plan future research directions. ..
  20. INTESTINAL AND PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN
    Neil Simister; Fiscal Year: 1999
    ....
  21. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2006
    ..Furthermore, since homologues of N. crassa G-alpha genes are required for virulence in numerous fungal species, these investigations will also lead to new therapies for emerging fungal pathogens. ..
  22. G PROTEINS AND SIGNAL TRANSDUCTION IN NEUROSPORA CRASSA
    KATHERINE BORKOVICH; Fiscal Year: 2003
    ..Furthermore, since homologues of N. crassa G-alpha genes are required for virulence in numerous fungal species, these investigations will also lead to new therapies for emerging fungal pathogens. ..
  23. PROTEINS INVOLVED IN TRANSPORT THROUGH PLASMODESMATA
    Vitaly Citovsky; Fiscal Year: 1999
    ..The intercellular localization of the MP kinase will be studied in detail, its expression patterns will be examined during plant development, and the plant gene coding for this enzyme will be cloned. ..
  24. PROTEINS INVOLVED IN TRANSPORT THROUGH PLASMODESMATA
    Vitaly Citovsky; Fiscal Year: 2000
    ..The intercellular localization of the MP kinase will be studied in detail, its expression patterns will be examined during plant development, and the plant gene coding for this enzyme will be cloned. ..
  25. Phospholipid-Mediated Metabolic Control in Liver and Brown Adipose Tissue
    David E Cohen; Fiscal Year: 2011
    ..These studies are expected to identify new therapeutic targets for the management of for NAFLD, type 2 diabetes and other obesity-associated disorders. ..
  26. Functional Analysis of an hnRNP C-Like Protein, p542
    JAMES MCAFEE; Fiscal Year: 2004
    ..The in-vitro telomere binding specificity of p542, parallels inferences linking hnRNP C to telomeres, and prompts our investigations into the in vivo association of p542 with telomeric DNA. ..
  27. EWS-FLI INTERACTING PROTEINS IN EWING'S SARCOMA
    Suzanne Baker; Fiscal Year: 2000
    ....
  28. EWS-FLI INTERACTING PROTEINS IN EWING'S SARCOMA
    Suzanne Baker; Fiscal Year: 1999
    ....
  29. A systems level analysis of spore coat assembly in Bacillus subtilis
    Patrick Eichenberger; Fiscal Year: 2010
    ..We anticipate that our results will have an impact on the design of therapeutic approaches to detect and eliminate pathogenic spore-forming bacteria. ..
  30. A systems level analysis of spore coat assembly in Bacillus subtilis
    Patrick Eichenberger; Fiscal Year: 2009
    ..We anticipate that our results will have an impact on the design of therapeutic approaches to detect and eliminate pathogenic spore-forming bacteria. ..
  31. MOLECULAR BASIS OF IMMUNOGLOBULIN HEAVY CHAIN SWITCH
    Janet Stavnezer; Fiscal Year: 2004
    ..In addition, mice with deficiencies in proteins already known to interact with MMR proteins, but to remain viable, will be tested for their ability to undergo CSR. ..
  32. MOLECULAR BASIS OF IMMUNOGLOBULIN HEAVY CHAIN SWITCH
    Janet Stavnezer; Fiscal Year: 2003
    ..In addition, mice with deficiencies in proteins already known to interact with MMR proteins, but to remain viable, will be tested for their ability to undergo CSR. ..
  33. MOLECULAR BASIS OF IMMUNOGLOBULIN HEAVY CHAIN SWITCH
    Janet Stavnezer; Fiscal Year: 2002
    ..In addition, mice with deficiencies in proteins already known to interact with MMR proteins, but to remain viable, will be tested for their ability to undergo CSR. ..
  34. MOLECULAR BASIS OF IMMUNOGLOBULIN HEAVY CHAIN SWITCH
    Janet Stavnezer; Fiscal Year: 2000
    ..In addition, mice with deficiencies in proteins already known to interact with MMR proteins, but to remain viable, will be tested for their ability to undergo CSR. ..
  35. MOLECULAR BASIS OF IMMUNOGLOBULIN HEAVY CHAIN SWITCH
    Janet Stavnezer; Fiscal Year: 2001
    ..In addition, mice with deficiencies in proteins already known to interact with MMR proteins, but to remain viable, will be tested for their ability to undergo CSR. ..
  36. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan Golden; Fiscal Year: 2004
    ....
  37. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan Golden; Fiscal Year: 2003
    ....
  38. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan Golden; Fiscal Year: 2004
    ....
  39. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan Golden; Fiscal Year: 2001
    ....
  40. THE PATHWAY THAT SETS THE CYANOBACTERIAL CIRCADIAN CLOCK
    Susan Golden; Fiscal Year: 2002
    ....
  41. Roles of Lig3 and XRCC1 Genes in Genome Stability
    Alan Tomkinson; Fiscal Year: 2009
    ..These differences between normal and cancer cells offer an opportunity to develop therapeutic strategies that selectively target cancer cells. ..
  42. The Role of RECQL4 in Bone Development and Osteosarcoma
    Lisa L Wang; Fiscal Year: 2010
    ..This will allow us to discover novel ways to improve the treatment of osteosarcoma and osteoporosis in the general population. ..
  43. Mechanisms of HIV-1 Variation
    LOUIS MANSKY; Fiscal Year: 2006
    ..Finally, cell culture experiments will be done to test mutations in either Vpr or reverse transcriptase that can alter the in vivo fidelity of reverse transcription. ..
  44. DEVELOPMENT OF MEMBRANE GLYCOPROTEIN STRUCTURE
    Philip Thomas; Fiscal Year: 1999
    ..The proposed studies are both necessary and fundamental to understanding the development of membrane protein structure, and may provide novel information relevant to several human diseases. ..
  45. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol Lim; Fiscal Year: 2009
    ..Our long-term, ultimate goal is to use localization controllable versions of Bcr-Abl (as gene therapy) for treatment of CML. ..
  46. Converting an Oncogene to an Apoptotic Factor by Manipulating Signal Sequences
    Carol S Lim; Fiscal Year: 2010
    ..Our long-term, ultimate goal is to use localization controllable versions of Bcr-Abl (as gene therapy) for treatment of CML. ..
  47. REGULATION OF MALTOSE FERMENTATION IN SACCHAROMYCES
    CORINNE MICHELS; Fiscal Year: 1999
    ..Mutations which broaden inducer specificity will be isolated. The alpha-methylglucoside-activator gene will be isolated and used to characterize the inducer sensing- signaling pathway. ..
  48. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2006
    ..abstract_text> ..
  49. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2005
    ..abstract_text> ..
  50. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2003
    ..abstract_text> ..
  51. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2003
    ..abstract_text> ..
  52. Characterization of Neuroendocrine CGRP Receptors
    Ian Dickerson; Fiscal Year: 2004
    ..abstract_text> ..
  53. C/EBP ALPHA MEDIATED REGULATION OF P21/SDI-1
    Nikolai Timchenko; Fiscal Year: 2000
    ..For this goal we will clone the genes that are responsible for C/EBPalpha-mediated growth arrest. ..
  54. C/EBP ALPHA MEDIATED REGULATION OF P21/SDI-1
    Nikolai Timchenko; Fiscal Year: 2001
    ..For this goal we will clone the genes that are responsible for C/EBPalpha-mediated growth arrest. ..
  55. C/EBP ALPHA MEDIATED REGULATION OF P21/SDI-1
    Nikolai Timchenko; Fiscal Year: 1999
    ..For this goal we will clone the genes that are responsible for C/EBPalpha-mediated growth arrest. ..
  56. Functional analysis of Helicobacter pylori VacA toxin
    Victor Torres; Fiscal Year: 2003
    ..To fulfill this aim I am using the VacA recombinant system to determine the minimum amino acid sequence required for the delta6-27-VacA toxin to exhibit a dominant-negative phenotype. ..
  57. HYPOHIDROTIC ECTODERMAL DYSPLASIA--A GENETIC ANALYSIS
    Jonathan Zonana; Fiscal Year: 2002
    ..At the same time, the new knowledge should rapidly result in improved genetic diagnosis and counseling, and may even suggest avenues for future therapies. ..
  58. HYPOHIDROTIC ECTODERMAL DYSPLASIA--A GENETIC ANALYSIS
    Jonathan Zonana; Fiscal Year: 2001
    ..At the same time, the new knowledge should rapidly result in improved genetic diagnosis and counseling, and may even suggest avenues for future therapies. ..
  59. HYPOHIDROTIC ECTODERMAL DYSPLASIA--A GENETIC ANALYSIS
    Jonathan Zonana; Fiscal Year: 1999
    ..At the same time, the new knowledge should rapidly result in improved genetic diagnosis and counseling, and may even suggest avenues for future therapies. ..
  60. HYPOHIDROTIC ECTODERMAL DYSPLASIA--A GENETIC ANALYSIS
    Jonathan Zonana; Fiscal Year: 2000
    ..At the same time, the new knowledge should rapidly result in improved genetic diagnosis and counseling, and may even suggest avenues for future therapies. ..
  61. ACTIVATION MECHANISMS IN THE NADPH OXIDASE
    Michael Kleinberg; Fiscal Year: 2001
    ..These findings may lead to new strategies for design of novel anti-inflammatory agents that will inhibit oxidase formation and production of superoxide by phagocytes. ..
  62. ACTIVATION MECHANISMS IN THE NADPH OXIDASE
    Michael Kleinberg; Fiscal Year: 2003
    ..These findings may lead to new strategies for design of novel anti-inflammatory agents that will inhibit oxidase formation and production of superoxide by phagocytes. ..
  63. ACTIVATION MECHANISMS IN THE NADPH OXIDASE
    Michael Kleinberg; Fiscal Year: 2000
    ..These findings may lead to new strategies for design of novel anti-inflammatory agents that will inhibit oxidase formation and production of superoxide by phagocytes. ..
  64. ACTIVATION MECHANISMS IN THE NADPH OXIDASE
    Michael Kleinberg; Fiscal Year: 2002
    ..These findings may lead to new strategies for design of novel anti-inflammatory agents that will inhibit oxidase formation and production of superoxide by phagocytes. ..
  65. Roles of Lig3 and XRCC1 Genes in Genome Stability
    Alan E Tomkinson; Fiscal Year: 2010
    ..These differences between normal and cancer cells offer an opportunity to develop therapeutic strategies that selectively target cancer cells. ..
  66. Characterization of Chlamydial Inclusion Migration
    Scott Grieshaber; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  67. Characterization of Chlamydial Inclusion Migration
    Scott Grieshaber; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  68. DIMERIZATION & COOPERATIVITY IN PROKARYOTIC GENE CONTROL
    Ann Hochschild; Fiscal Year: 2000
    ..This assay will also be applied to the study and manipulation of a phosphorylation-dependent protein-protein interaction involving the eukaryotic transcription factor CREB and its CREB and its co-activator CBP. ..
  69. Signaling Mechanisms Behind nAChR Clustering at the NMJ
    Jenny Linnoila; Fiscal Year: 2007
    ..Thus, studying the fundamental process of neuromuscular development is critical to understanding the causes of and to designing more effective treatments for these debilitating conditions. [unreadable] [unreadable] [unreadable]..
  70. DIMERIZATION & COOPERATIVITY IN PROKARYOTIC GENE CONTROL
    Ann Hochschild; Fiscal Year: 2001
    ..This assay will also be applied to the study and manipulation of a phosphorylation-dependent protein-protein interaction involving the eukaryotic transcription factor CREB and its CREB and its co-activator CBP. ..
  71. DIMERIZATION & COOPERATIVITY IN PROKARYOTIC GENE CONTROL
    Ann Hochschild; Fiscal Year: 2002
    ..This assay will also be applied to the study and manipulation of a phosphorylation-dependent protein-protein interaction involving the eukaryotic transcription factor CREB and its CREB and its co-activator CBP. ..
  72. THE ROLE OF UFD1 AND CDC45 IN NEURAL CREST DEVELOPMENT
    Vidu Garg; Fiscal Year: 2001
    ..The function of Cdc45 will be examined in vivo in chick embryos by retrovirally mediated alteration of gene expression. A yeast two hybrid assay will be utilized to determine if Ufdl, Cdc45, and Hira interact with one another. ..
  73. THE ROLE OF UFD1 AND CDC45 IN NEURAL CREST DEVELOPMENT
    Vidu Garg; Fiscal Year: 2003
    ..The function of Cdc45 will be examined in vivo in chick embryos by retrovirally mediated alteration of gene expression. A yeast two hybrid assay will be utilized to determine if Ufdl, Cdc45, and Hira interact with one another. ..
  74. THE ROLE OF UFD1 AND CDC45 IN NEURAL CREST DEVELOPMENT
    Vidu Garg; Fiscal Year: 2002
    ..The function of Cdc45 will be examined in vivo in chick embryos by retrovirally mediated alteration of gene expression. A yeast two hybrid assay will be utilized to determine if Ufdl, Cdc45, and Hira interact with one another. ..