northern blotting

Summary

Summary: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Top Publications

  1. ncbi An integrated stress response regulates amino acid metabolism and resistance to oxidative stress
    Heather P Harding
    Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 11:619-33. 2003
  2. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
  3. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
  4. ncbi Novel small RNA-encoding genes in the intergenic regions of Escherichia coli
    L Argaman
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, 91120, Jerusalem, Israel
    Curr Biol 11:941-50. 2001
  5. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
  6. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
  7. ncbi MicroRNAs modulate hematopoietic lineage differentiation
    Chang Zheng Chen
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 303:83-6. 2004
  8. pmc Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells
    Xuezhong Cai
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:5570-5. 2005
  9. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
  10. ncbi Vascular endothelial growth factor is a secreted angiogenic mitogen
    D W Leung
    Department of Molecular Biology, Genetech, South San Francisco, CA 94080
    Science 246:1306-9. 1989

Detail Information

Publications344 found, 100 shown here

  1. ncbi An integrated stress response regulates amino acid metabolism and resistance to oxidative stress
    Heather P Harding
    Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 11:619-33. 2003
    ..A signaling pathway initiated by eIF2alpha phosphorylation protects cells against metabolic consequences of ER oxidation by promoting the linked processes of amino acid sufficiency and resistance to oxidative stress...
  2. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
    ..elegans larval development, and three of them have apparent homologs in mammals and/or insects. Small noncoding RNAs of the miRNA class appear to be numerous and diverse...
  3. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
    ..This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated...
  4. ncbi Novel small RNA-encoding genes in the intergenic regions of Escherichia coli
    L Argaman
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, 91120, Jerusalem, Israel
    Curr Biol 11:941-50. 2001
    ..Thus, in spite of the rising recognition that such RNAs may play key roles in bacterial physiology, many of the small RNAs known to date were discovered fortuitously...
  5. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
    ..The abundance of these tiny RNAs, their expression patterns, and their evolutionary conservation imply that, as a class, miRNAs have broad regulatory functions in animals...
  6. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
    ....
  7. ncbi MicroRNAs modulate hematopoietic lineage differentiation
    Chang Zheng Chen
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 303:83-6. 2004
    ..Our results indicate that microRNAs are components of the molecular circuitry that controls mouse hematopoiesis and suggest that other microRNAs have similar regulatory roles during other facets of vertebrate development...
  8. pmc Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells
    Xuezhong Cai
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:5570-5. 2005
    ..We hypothesize that these viral miRNAs play a critical role in the establishment and/or maintenance of KSHV latent infection in vivo and, hence, in KSHV-induced oncogenesis...
  9. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
  10. ncbi Vascular endothelial growth factor is a secreted angiogenic mitogen
    D W Leung
    Department of Molecular Biology, Genetech, South San Francisco, CA 94080
    Science 246:1306-9. 1989
    ..Human 293 cells transfected with an expression vector containing a bovine or human VEGF cDNA insert secrete an endothelial cell mitogen that behaves like native VEGF...
  11. pmc A microRNA array reveals extensive regulation of microRNAs during brain development
    Anna M Krichevsky
    Center for Neurologic Diseases, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    RNA 9:1274-81. 2003
    ..Creating clusters of coexpressed miRNAs will contribute to understanding their regulation, functions, and discovery of mRNA targets...
  12. pmc Microarray analysis of microRNA expression in the developing mammalian brain
    Eric A Miska
    Howard Hughes Medical Institute, Department of Biology and McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genome Biol 5:R68. 2004
    ..In invertebrates microRNAs have been implicated as regulators of developmental timing, neuronal differentiation, cell proliferation, programmed cell death and fat metabolism. Little is known about the roles of microRNAs in mammals...
  13. ncbi MicroRNA detection by northern blotting using locked nucleic acid probes
    Eva Várallyay
    Agricultural Biotechnology Center, Plant Virology Group, Szent Gyorgyi Albert ut 4, Gödöllõ H 2100, Hungary
    Nat Protoc 3:190-6. 2008
    ..for miRNA northern blot analysis, which includes RNA extraction, polyacrylamide gel electrophoresis and northern blotting, and the hybridization and detection of locked nucleic acid (LNA)-modified oligonucleotide probes...
  14. ncbi Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues
    S Lenzen
    Institute of Clinical Biochemistry, Hannover Medical School, Germany
    Free Radic Biol Med 20:463-6. 1996
    ....
  15. pmc Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia
    George Adrian Calin
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:15524-9. 2002
    ..Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases...
  16. ncbi Differential gene expression by Paracoccidioides brasiliensis in host interaction conditions: representational difference analysis identifies candidate genes associated with fungal pathogenesis
    Alexandre Melo Bailão
    Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade Federal de Goias, 74001 970 Goiania, Goias, Brazil
    Microbes Infect 8:2686-97. 2006
    ..This work can facilitate functional studies of novel regulated genes that may be important for the survival and growth strategies of P. brasiliensis in humans...
  17. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage...
  18. pmc Role of Leishmania (Leishmania) chagasi amastigote cysteine protease in intracellular parasite survival: studies by gene disruption and antisense mRNA inhibition
    Vasanthakrishna Mundodi
    Department of Biological Sciences, University of Calgary, Calgary AB T2N1N4, Canada
    BMC Mol Biol 6:3. 2005
    ..chagasi. Here, we have further characterized this cysteine protease gene and demonstrated its role during infection and survival of Leishmania (L.) chagasi within the U937 macrophage cells...
  19. ncbi Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA
    Ranhui Duan
    Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Hum Mol Genet 16:1124-31. 2007
    ..Thus, our study reveals an additional structural requirement for pri-miRNA processing and emphasizes the importance of identifying new miRNA SNPs and their contributions to miRNA biogenesis and human genetic disease...
  20. pmc MicroRNA-binding viral protein interferes with Arabidopsis development
    Padmanabhan Chellappan
    International Laboratory for Tropical Agricultural Biotechnology, Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    Proc Natl Acad Sci U S A 102:10381-6. 2005
    ....
  21. pmc Identification of novel small RNAs using comparative genomics and microarrays
    K M Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 15:1637-51. 2001
    ..The approaches used here should allow identification of small RNAs in other organisms...
  22. ncbi Patterns of Nogo mRNA and protein expression in the developing and adult rat and after CNS lesions
    Andrea B Huber
    Brain Research Institute, University of Zurich, Zurich, 8057 Switzerland
    J Neurosci 22:3553-67. 2002
    ....
  23. ncbi A novel androgen-regulated gene, PMEPA1, located on chromosome 20q13 exhibits high level expression in prostate
    L L Xu
    Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, Bethesda, Maryland, 20814 4799, USA
    Genomics 66:257-63. 2000
    ..PMEPA1, along with other highly androgen-induced prostate-specific genes, has potential to serve as an androgen signaling read-out biomarker in prostate tissue...
  24. ncbi Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition
    Xuemei Wu
    Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Genet 33:187-91. 2003
    ..Thus, Zar1 is the first identified oocyte-specific maternal-effect gene that functions at the oocyte-to-embryo transition and, as such, offers new insights into the initiation of embryonic development and fertility control in mammals...
  25. ncbi E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo
    B T Porse
    Laboratory of Gene Therapy Research, 2100 Copenhagen Ø, Denmark
    Cell 107:247-58. 2001
    ....
  26. pmc Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue
    X Liu
    Laboratory of Biochemistry and Metabolism, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 92:8831-5. 1995
    ..The increase of Stat5 expression during pregnancy coincides with the activation of the WAP gene...
  27. ncbi Characterization of an 18,166 EST dataset for cassava (Manihot esculenta Crantz) enriched for drought-responsive genes
    Y Lokko
    Central Biotechnology Laboratory, International Institute of Tropical Agriculture, Oyo Road, Ibadan, Nigeria
    Plant Cell Rep 26:1605-18. 2007
    ....
  28. pmc The G-protein alpha-subunit GasC plays a major role in germination in the dimorphic fungus Penicillium marneffei
    Sophie Zuber
    Department of Genetics, University of Melbourne, 3010 Victoria, Australia
    Genetics 164:487-99. 2003
    ..In contrast to the S. cerevisiae ortholog Gpa2, our data indicate that GasC is not involved in carbon or nitrogen source sensing and plays no major role in either hyphal or yeast growth or in the switch between these two forms...
  29. pmc A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Genes Dev 18:2491-505. 2004
    ..These findings suggest that nutrient signals set the critical cell-size threshold via Sfp1 and Sch9-mediated control of ribosome biosynthetic rates...
  30. ncbi Autophagy is required for maintenance of amino acid levels and protein synthesis under nitrogen starvation
    Jun Onodera
    Division of Molecular Cell Biology, National Institute for Basic Biology, and Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies, Nishigonaka 38, Myodaiji cho, Okazaki 444 8585, Japan
    J Biol Chem 280:31582-6. 2005
    ..Autophagy-defective cells fail to maintain physiologic amino acid levels, and their inability to synthesize new proteins may explain most phenotypes associated with autophagy mutants at least partly...
  31. ncbi MicroRNAs and other tiny endogenous RNAs in C. elegans
    Victor Ambros
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Curr Biol 13:807-18. 2003
    ..We employed cDNA sequencing and comparative genomics to identify additional C. elegans small RNAs with properties similar to miRNAs and siRNAs...
  32. ncbi NDRG1 is necessary for p53-dependent apoptosis
    Susanne Stein
    Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Biol Chem 279:48930-40. 2004
    ..This report further supports the notion that p53 controls a network of genes that are required for its apoptotic function...
  33. ncbi Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans
    C Reynet
    Research Division, Joslin Diabetes Center, Boston, MA
    Science 262:1441-4. 1993
    ..Messenger ribonucleic acid of Rad was expressed primarily in skeletal and cardiac muscle and was increased an average of 8.6-fold in the muscle of Type II diabetics as compared to normal individuals...
  34. pmc A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    Adam Grundhoff
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
    RNA 12:733-50. 2006
    ..The advantages and limitations of the approach are discussed...
  35. ncbi Mechanism of killer gene activation. Antisense RNA-dependent RNase III cleavage ensures rapid turn-over of the stable hok, srnB and pndA effector messenger RNAs
    K Gerdes
    Department of Molecular Biology, Odense University, Denmark
    J Mol Biol 226:637-49. 1992
    ..Based on these and on observations obtained previously, we present a molecular model that explains the activation of the killer mRNAs in plasmid-free segregants and after addition of rifampicin...
  36. ncbi A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper
    Sun Hwa Ha
    National Institute of Agricultural Biotechnology, RDA, Suwon 441 707, Republic of Korea
    J Exp Bot 58:3135-44. 2007
    ....
  37. ncbi Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs
    N Porchet
    Unité 16 INSERM, Lille, France
    Biochem Biophys Res Commun 175:414-22. 1991
    ..This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3...
  38. ncbi The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis
    D Finley
    Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115
    Nature 338:394-401. 1989
    ..These results suggest a novel 'chaperone' function for ubiquitin, in which its covalent association with other proteins promotes the formation of specific cellular structures...
  39. ncbi Discovery in silico and characterization in vitro of novel genes exclusively expressed in the mouse epididymis
    Jenni Penttinen
    Department of Physiology, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Mol Endocrinol 17:2138-51. 2003
    ..However, the expression of AV381130 was not detected until d 30 after birth, indicating a close connection between gene expression and puberty...
  40. ncbi TGF-beta 1 binding protein: a component of the large latent complex of TGF-beta 1 with multiple repeat sequences
    T Kanzaki
    Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden
    Cell 61:1051-61. 1990
    ..TGF-beta 1-BP was found not to bind and inactive TGF-beta 1; its role in the latent complex is discussed...
  41. pmc CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus
    A Rowley
    Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada
    Mol Cell Biol 11:5718-26. 1991
    ....
  42. pmc Characterization of Dicer-deficient murine embryonic stem cells
    Elizabeth P Murchison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 102:12135-40. 2005
    ..These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells...
  43. pmc RNomics in Escherichia coli detects new sRNA species and indicates parallel transcriptional output in bacteria
    Jörg Vogel
    Institute of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, 75124 Uppsala, Sweden
    Nucleic Acids Res 31:6435-43. 2003
    ..The experimental characterization of sRNAs analyzed here suggests that the definition of an sRNA is more complex than previously assumed...
  44. ncbi The roles of Toll-like receptor 9, MyD88, and DNA-dependent protein kinase catalytic subunit in the effects of two distinct CpG DNAs on dendritic cell subsets
    Hiroaki Hemmi
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University and Solution Oriented Research for Science and Technology, Japan Science and Technology Corp, Suita, Osaka, Japan
    J Immunol 170:3059-64. 2003
    ..This study demonstrates that the TLR9-MyD88 signaling pathway is essential for all DC responses to both types of CpG DNAs...
  45. ncbi Paraoxonase-2 is a ubiquitously expressed protein with antioxidant properties and is capable of preventing cell-mediated oxidative modification of low density lipoprotein
    C J Ng
    Atherosclerosis Research Unit, University of California, Los Angeles, California 90095 1679, USA
    J Biol Chem 276:44444-9. 2001
    ..However, in contrast to PON1 and PON3, PON2 may exert its antioxidant functions at the cellular level, joining the host of intracellular antioxidant enzymes that protect cells from oxidative stress...
  46. ncbi Cloning and functional expression of a human kidney Na+:HCO3- cotransporter
    C E Burnham
    Department of Internal Medicine, University of Cincinnati School of Medicine, Cincinnati, Ohio 45267 0585, USA
    J Biol Chem 272:19111-4. 1997
    ..6-kilobase mRNA is highly expressed in kidney and pancreas, with detectable levels in brain. Functional studies in transiently transfected HEK-293 cells demonstrate that the cloned transporter mediates Na+:HCO3- cotransport...
  47. ncbi P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction
    Kristin D Kasschau
    Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
    Dev Cell 4:205-17. 2003
    ..The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway...
  48. ncbi A facile lentiviral vector system for expression of doxycycline-inducible shRNAs: knockdown of the pre-miRNA processing enzyme Drosha
    Lars Aagaard
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Mol Ther 15:938-45. 2007
    ..We anticipate that our vector will facilitate functional studies of miRNA biogenesis...
  49. pmc Sequential protein association with nascent 60S ribosomal particles
    Cosmin Saveanu
    Genetique des Interactions Macromoleculaires, CNRS URA2171 PT Protéomique, Institut Pasteur, 75724 Paris Cedex 15, France
    Mol Cell Biol 23:4449-60. 2003
    ..Physical and functional interactions between Rlp24 and Nog1 revealed that the assembly steps are, at least in part, mediated by direct protein-protein interactions...
  50. ncbi The IC-SNURF-SNRPN transcript serves as a host for multiple small nucleolar RNA species and as an antisense RNA for UBE3A
    M Runte
    Institut fur Humangenetik, Universitatsklinikum Essen, 45122 Essen, Germany
    Hum Mol Genet 10:2687-700. 2001
    ..As we have not obtained any evidence for other genes in this region, which, from the mouse data appears to be critical for the neonatal Prader-Willi syndrome phenotype, a lack of these snoRNAs may be causally involved in this disease...
  51. ncbi Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display
    Masato Yamada
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan
    FEMS Microbiol Lett 254:165-72. 2006
    ..Seventeen cDNAs were not similar to proteins in the database and may represent unique genes that play specific roles in the process of fruiting in F. velutipes...
  52. pmc The microRNAs of Caenorhabditis elegans
    Lee P Lim
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Genes Dev 17:991-1008. 2003
    ..Our census of the worm miRNAs and their expression patterns helps define this class of noncoding RNAs, lays the groundwork for functional studies, and provides the tools for more comprehensive analyses of miRNA genes in other species...
  53. ncbi Oogenesin is a novel mouse protein expressed in oocytes and early cleavage-stage embryos
    Naojiro Minami
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Biol Reprod 69:1736-42. 2003
    ..These results suggest that the gene has some roles in zygotic transcription of early preimplantation embryos as well as folliculogenesis and oogenesis in the mouse...
  54. pmc Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response
    Phoebe D Lu
    Department of Cell Biology, School of Medicine, New York University, New York, NY 10016, USA
    J Cell Biol 167:27-33. 2004
    ..These features are common to regulated translation of GCN4 in yeast. The metazoan ISR thus resembles the yeast general control response both in its target genes and its mechanistic details...
  55. ncbi Engineering plants with increased levels of the antioxidant chlorogenic acid
    Ricarda Niggeweg
    John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
    Nat Biotechnol 22:746-54. 2004
    ..Tomatoes with elevated CGA levels could be used in foods with specific benefits for human health...
  56. pmc Complex molecular architecture of beet yellows virus particles
    Valera V Peremyslov
    Department of Botany and Plant Pathology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331, USA
    Proc Natl Acad Sci U S A 101:5030-5. 2004
    ..The unexpectedly complex structure of closterovirus virions has important mechanistic and functional implications that may also apply to other virus families...
  57. pmc Involvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora
    Caterina Catalanotto
    Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Universita di Roma La Sapienza, 00161 Roma, Italy
    Genes Dev 16:790-5. 2002
    ..Supporting this idea, a purified QDE2 protein complex was found to contain small RNA molecules, suggesting that QDE2 could be part of a small RNA-directed ribonuclease complex involved in sequence-specific mRNA degradation...
  58. ncbi Cloning and characterization of a novel serine/threonine protein phosphatase type 5 from Trypanosoma brucei
    M Chaudhuri
    Department of Microbiology, Meharry Medical College, Nashville, TN 37208, USA
    Gene 266:1-13. 2001
    ..brucei...
  59. ncbi Lysozyme from the gut of the soft tick Ornithodoros moubata: the sequence, phylogeny and post-feeding regulation
    Lenka Grunclová
    Faculty of Biological Sciences, Institute of Parasitology ASCR, Academy of Sciences of the Czech Republic, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic
    Dev Comp Immunol 27:651-60. 2003
    ..Semi-quantitative RT-PCR and Northern blotting analysis demonstrated that TGL is strongly up-regulated at the transcriptional level after a bloodmeal...
  60. ncbi The effects of angiopoietin-1 and -2 on tumor growth and angiogenesis in human colon cancer
    S A Ahmad
    Department of Surgical Oncology, The University of Texas M. D. Anderson Cancer Center, Houston 77030, USA
    Cancer Res 61:1255-9. 2001
    ..These data suggest that imbalances between Ang-1 and -2 that result in a net gain of Ang-2 activity lead to enhanced tumor angiogenesis and growth...
  61. pmc Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses
    Tadas Panavas
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Proc Natl Acad Sci U S A 102:7326-31. 2005
    ..This finding suggests that virus infection and disease-causing proteins might use or alter similar host pathways and may suggest connections between chronic diseases and prior virus infection...
  62. pmc sRNAPredict: an integrative computational approach to identify sRNAs in bacterial genomes
    Jonathan Livny
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, MA 02111, USA
    Nucleic Acids Res 33:4096-105. 2005
    ..Our findings suggest that sRNAPredict can be used to efficiently identify novel sRNAs even in bacteria for which promoter consensus sequences are not available...
  63. pmc Nuclear export and cytoplasmic processing of precursors to the 40S ribosomal subunits in mammalian cells
    Jacques Rouquette
    Laboratoire de Biologie Moléculaire des Eucaryotes and Institut d Exploration Fonctionnelle des Génomes, CNRS and Université Paul Sabatier, Toulouse Cedex, France
    EMBO J 24:2862-72. 2005
    ..It also allows new strategies for the study of preribosomal transport in mammalian cells...
  64. ncbi The chemotactic receptor for human C5a anaphylatoxin
    N P Gerard
    Ina Sue Perlmutter Research Laboratory, Childrens Hospital, Boston, Massachusetts
    Nature 349:614-7. 1991
    ..The deduced amino-acid sequence of the receptor reveals the expected motifs befitting its interaction with cellular GTP-binding proteins...
  65. pmc A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
    Tao Zhao
    National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Genes Dev 21:1190-203. 2007
    ..Our findings suggest that the miRNA pathway and some siRNA pathways are ancient mechanisms of gene regulation that evolved prior to the emergence of multicellularity...
  66. ncbi Repression of farnesoid X receptor during the acute phase response
    Min Sun Kim
    Department of Medicine, University of California San Francisco, Metabolism Section, Medical Service, Department of Veterans Affairs Medical Center, San Francisco, California 94121, USA
    J Biol Chem 278:8988-95. 2003
    ....
  67. pmc A truncated laminin chain homologous to the B2 chain: structure, spatial expression, and chromosomal assignment
    P Kallunki
    Department of Biochemistry, University of Oulu, Finland
    J Cell Biol 119:679-93. 1992
    ..By in situ hybridization expression of the B2t chain was localized to specific epithelial cells in skin, lung, and kidney as opposed to a general epithelial and endothelial cell expression of the laminin B2 chain in the same tissues...
  68. pmc cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes
    G Manfioletti
    Universita di Trieste, Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Italy
    Nucleic Acids Res 19:6793-7. 1991
    ..Analysis of mRNA levels demonstrate that the HMGI-C gene is not expressed in a variety of mouse tissues but is expressed in Lewis lung carcinoma cells...
  69. ncbi Prostate-specific and androgen-dependent expression of a novel homeobox gene
    C J Bieberich
    Department of Virology, Jerome H Holland Laboratory, Rockville, Maryland 20855, USA
    J Biol Chem 271:31779-82. 1996
    ..1 protein, which likely functions as a transcription factor, plays a prominent role both in the initiation of prostate development and in the maintenance of the differentiated state of prostatic epithelial cells...
  70. ncbi Laminin alpha 2 is a component of brain capillary basement membrane: reduced expression in dystrophic dy mice
    M Jucker
    Molecular Neurobiology Unit, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Neuroscience 71:1153-61. 1996
    ..This observation might be explained by the fact that the cerebral capillary basement membrane expresses both alpha chains and therefore exhibits structural redundancy...
  71. ncbi Identification of additional genes belonging to the LexA regulon in Escherichia coli
    A R Fernandez de Henestrosa
    Section on DNA Replication, Repair and Mutagenesis, Building 6, Room 1A13, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2725, USA
    Mol Microbiol 35:1560-72. 2000
    ..These experiments have allowed us to identify seven new LexA-regulated genes, thus bringing the present number of genes in the E. coli LexA regulon to 31. The potential function of each newly identified LexA-regulated gene is discussed...
  72. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
    ..Our results provide important new insights into how the BMP/Smad pathway can specifically activate target genes...
  73. ncbi Suprabasal expression of a dominant-negative RXR alpha mutant in transgenic mouse epidermis impairs regulation of gene transcription and basal keratinocyte proliferation by RAR-selective retinoids
    X Feng
    Department of Dermatology, University of Michigan, Ann Arbor 48109 0314, USA
    Genes Dev 11:59-71. 1997
    ....
  74. ncbi Cloning of the LamA3 gene encoding the alpha 3 chain of the adhesive ligand epiligrin. Expression in wound repair
    M C Ryan
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    J Biol Chem 269:22779-87. 1994
    ..In contrast, epiligrin expression was up-regulated in wound sites of human skin...
  75. ncbi Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq
    Hiroshi Kawamoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 61:1013-22. 2006
    ..We also showed in vitro that SgrS forms a stable duplex with the ptsG mRNA, and that Hfq markedly facilitates the rate of duplex formation...
  76. ncbi A STAT-regulated, stress-induced signalling pathway in Dictyostelium
    Tsuyoshi Araki
    School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dow Street, Dundee, DD1 5EH, UK
    J Cell Sci 116:2907-15. 2003
    ..These data show that Dd-STATc functions as a transcriptional activator in a stress-response pathway and the pharmacological evidence, at least, is consistent with cGMP acting as a second messenger...
  77. ncbi The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae
    M Tucker
    Department of Molecular and Cellular Biology and, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Cell 104:377-86. 2001
    ..Interestingly, because Ccr4p and Caf1p interact with transcription factors, these results suggest an unexpected link between mRNA synthesis and turnover...
  78. pmc Calcineurin is essential for survival during membrane stress in Candida albicans
    M Cristina Cruz
    Department of Genetics, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 21:546-59. 2002
    ..albicans mutants, against other Candida species, or when combined with different azoles. We propose that calcineurin is part of a membrane stress survival pathway that could be targeted for therapy...
  79. ncbi The noncoding RNA taurine upregulated gene 1 is required for differentiation of the murine retina
    T L Young
    Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 15:501-12. 2005
    ..However, of the thousands of ncRNAs identified, the functions of relatively few have been established...
  80. pmc Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome
    Timothy Ravasi
    ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    Genome Res 16:11-9. 2006
    ..Taken together, the data provide strong support for the conclusion that ncRNAs are an important, regulated component of the mammalian transcriptome...
  81. pmc A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis
    Rebecca L Brown
    Cooperative Research Centre for Tropical Plant Protection, Brisbane, Queensland 4072, Australia
    Plant Physiol 132:1020-32. 2003
    ....
  82. ncbi Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development
    Savraj S Grewal
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Nat Cell Biol 7:295-302. 2005
    ..In addition, the growth effects of dMyc in larval wing imaginal discs require de novo rRNA synthesis. We suggest that during animal development, the control of rRNA synthesis and ribosome biogenesis is an essential Myc function...
  83. ncbi 4'-phosphopantetheinyl transferase-encoding npgA is essential for siderophore biosynthesis in Aspergillus nidulans
    Harald Oberegger
    Department of Molecular Biology, University of Innsbruck, Peter Mayr Strasse 4b, 6020 Innsbruck, Austria
    Curr Genet 44:211-5. 2003
    ....
  84. ncbi A mammalian gene with introns instead of exons generating stable RNA products
    K T Tycowski
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 379:464-6. 1996
    ..Thus, the introns rather than the exons specify the functional products of UHG...
  85. pmc Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot
    Gurman Singh Pall
    Division of Cancer Sciences and Molecular Pathology, Glasgow University, Western Infirmary, Dumbarton Road, Glasgow, G11 6NT, UK
    Nucleic Acids Res 35:e60. 2007
    ..However, increasing the dose of UV reduced the detection of miRNA by hybridization in northern blotting experiments...
  86. ncbi Induction and expression of betaig-h3 in pancreatic cancer cells
    Dominik Schneider
    Department of Visceral and Transplantation Surgery, University of Bern, Bern, Switzerland
    Biochim Biophys Acta 1588:1-6. 2002
    ....
  87. ncbi The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(-/-) mice and results in a mouse with spinal muscular atrophy
    U R Monani
    Departments of Neurology, College of Medicine, Ohio State University, Columbus, OH 43210, USA
    Hum Mol Genet 9:333-9. 2000
    ..These results show that SMA is caused by insufficient SMN production by the SMN2 gene and that increased expression of the SMN2 gene may provide a strategy for treating SMA patients...
  88. ncbi The epithelin precursor encodes two proteins with opposing activities on epithelial cell growth
    G D Plowman
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121
    J Biol Chem 267:13073-8. 1992
    ..The broad expression profile of epithelin transcripts, along with the opposing activities of the two mature protein products, implicates these factors as natural mediators of epithelial homeostasis...
  89. ncbi Clinical significance of secreted protein acidic and rich in cystein in esophageal carcinoma and its relation to carcinoma progression
    Keishi Yamashita
    Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan
    Cancer 97:2412-9. 2003
    ..The goal of this study was to show the clinical relevance of SPARC and its relation to MMP-2 expression in esophageal carcinoma patients...
  90. ncbi Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7)
    L A Bobek
    Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214
    J Biol Chem 268:20563-9. 1993
    ..MG2 mRNA is about 2.5 kilobases long, and its expression appears to be species-, tissue-, and cell-specific. We propose to name this gene MUC7 in accordance with the mucin genes cloned to date named MUC1-MUC6...
  91. pmc Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains
    Christophe Pichon
    Biochimie Pharmaceutique, Universite de Rennes I, Unité Propre de Recherche de l Enseignement Supérieur JE 2311, Inserm ESPRI, 2 Avenue du Professeur Leon Bernard, 35043 Rennes, France
    Proc Natl Acad Sci U S A 102:14249-54. 2005
    ..Some of these RNAs show large variations of expression among pathogenic strains, suggesting that they are involved in the regulation of staphylococcal virulence...
  92. ncbi Stabilization of mRNA expression in whole blood samples
    Lynne Rainen
    PreAnalytiX CH c o Becton Dickinson, Franklin Lakes, NJ 07417, USA
    Clin Chem 48:1883-90. 2002
    ....
  93. pmc Regulation of transcription by H1 phosphorylation in Tetrahymena is position independent and requires clustered sites
    Yali Dou
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Proc Natl Acad Sci U S A 99:6142-6. 2002
    ..The results are explained by a nucleation-propagation model and provide in vivo evidence that the synergy of the clustered positive charges plays a role in histone function and gene regulation...
  94. pmc Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6
    B Dong
    Cold Spring Harbor Laboratory, NY 11724 2208
    Nucleic Acids Res 21:4085-92. 1993
    ....
  95. ncbi Molecular cloning of the mouse Ltbp-1 gene reveals tissue specific expression of alternatively spliced forms
    Irene Noguera
    Department of Cell Biology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Gene 308:31-41. 2003
    ..b>Northern blotting analyses indicate that long and short Ltbp-1 transcripts are widely expressed in adult mouse tissues and ..
  96. pmc MicroRNA-guided processing impairs Plum pox virus replication, but the virus readily evolves to escape this silencing mechanism
    Carmen Simón-Mateo
    Centro Nacional de Biotecnologia CSIC, Campus Universidad Autonoma de Madrid, Spain
    J Virol 80:2429-36. 2006
    ..Furthermore, we show that virus chimeras readily escape RNA silencing interference through mutations within the miRNA target sequence, which mainly affected nucleotides matching the 5'-terminal region of the miRNA...
  97. pmc Cell organisation, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells
    Rosângela V Andrade
    Depto de Biologia Celular, Universidade de Brasilia, 70910 900, Brasilia DF, Brazil
    BMC Genomics 7:208. 2006
    ..To advance our understanding of molecular mechanisms of dimorphic transition, we performed an extended analysis of gene expression profiles using the methods mentioned above...
  98. pmc Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein
    Glenn W Kaatz
    The John D Dingell Department of Veteran s Affairs Medical Center, Detroit, MI 48201, USA
    Antimicrob Agents Chemother 49:1857-64. 2005
    ..MepR overexpression reversed the MDR phenotypes of both mutants by repressing mepA transcription. All three ORFs are preferentially transcribed as a single mepRAB unit, suggesting that the three genes form an operon...
  99. ncbi Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4
    R Xu
    State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, 200433, People s Republic of China
    Genomics 62:537-9. 1999
    ..Northern blot analysis revealed that the mRNA of ADAM29 is highly expressed in the testis. By radiation hybrid panel mapping, the ADAM29 gene was assigned to human chromosome 4q34.2-qter...
  100. pmc Conversion of ectoderm into a neural fate by ATH-3, a vertebrate basic helix-loop-helix gene homologous to Drosophila proneural gene atonal
    K Takebayashi
    Department of Biological Sciences, Kyoto University Faculty of Medicine, Yoshida, Sakyo ku, Japan
    EMBO J 16:384-95. 1997
    ....
  101. pmc IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase
    Shyun Li
    Tularik, Inc, 2 Corporate Drive, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 99:5567-72. 2002
    ....

Research Grants62

  1. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2006
    ..RT-PCR (for screening) and northern blotting (for quantitation) will analyze Gene expression...
  2. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2010
    ....
  3. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2010
    ....
  4. Cloning and Analysis of a Gamete Fusion Gene
    CHARLENE FOREST; Fiscal Year: 2009
    ..Fluorescence and electron microscopy as well as Northern blotting and RT-PCR will be used to study the regulation, production and localization of this protein and ..
  5. Cytokines and the blood-brain barrier in the ovine fetus
    Barbara S Stonestreet; Fiscal Year: 2012
    ..The incidence of CP is 40-148/1,000 in premature and 1-2/1,000 in full term infants. This project could provide new insights into novel strategies to prevent brain injury in the human fetus and/or premature infant. ..
  6. Poly(ADP-ribose)Polymerase 1 Regulates Nucleolar Structure and Functions
    ERNEST KOJO BOAMAH; Fiscal Year: 2012
    ..This proposal will uncover a novel and exciting role for PARP1, which may have significant impact on cancer treatment regimens. ..
  7. Screening strategy for small molecules inhibitors of cancer
    Paul B Fisher; Fiscal Year: 2012
    ..These small molecule chemical probes validated from the secondary screens might have potential anticancer properties and could provide a foundation for the development of novel antitumor drugs. ..
  8. Theranostic nanoparticles to enhance morpholino delivery to the liver for suppres
    DAVID PETER CORMODE; Fiscal Year: 2011
    ..e. PCR, Western blotting and Northern blotting. This mentored phase will set the stage for the R00 independent phase where the nanoparticle development and ..
  9. The role of decapping factors in mRNP remodeling
    MARC ARRIBAS-LAYTON; Fiscal Year: 2013
    ..and mass spectrometry (MS) to identify interacting partners and pulse-chase experiments combined with Northern blotting and polysome profiling to determine mRNA stability and translational activity...
  10. Mechanisms of Homeostatic Control of Copper in Tissues
    LAWRENCE WILSON GRAY; Fiscal Year: 2011
    ..Relevance to public health: Understanding these questions would provide better insight into how the body balances copper levels. Additionally, SCC could be a unique indicator of WD. ..
  11. Characterizing regulatory RNAs in oral polymicrobial community
    PETER ALLAN JORTH; Fiscal Year: 2013
    ..These studies will help us better understand how oral bacteria thrive in polymicrobial communities that cause human disease. ..
  12. Bestrophin Channel Function in Heart
    FIONA CATHERINE BRITTON; Fiscal Year: 2012
    ..The proposed studies may provide novel insights into our understanding of the role of Bestrophin channels in cardiac physiology and disease states and thus provide potential areas for therapeutic targets. ..
  13. Theranostic nanoparticles to enhance morpholino delivery to the liver for suppres
    DAVID PETER CORMODE; Fiscal Year: 2013
    ..e. PCR, Western blotting and Northern blotting. This mentored phase will set the stage for the R00 independent phase where the nanoparticle development and ..
  14. A role for the miroRNA miR-133b in synapse development
    MARY PATRICIA HEYER; Fiscal Year: 2010
    ..This work will deepen our understanding of the complexities of synapse development, with future relevance for central synaptic disorders, which are of significant concern for public health. ..
  15. One-Step, POC Sample-to-Answer Process for RNA Analysis Outside the Laboratory
    John E Mueller; Fiscal Year: 2013
    ..The final product is envisaged to be an FDA-approved, CLIA-waived RNA-based sample-to-answer diagnostic device based on the process established in Phase I and II. ..
  16. Characterization of microRNAs expressed in the developing songbird brain
    Xiaoching Li; Fiscal Year: 2010
    ..g., autism, dyslexia, etc). ..
  17. Typhoon Trio Phosphor Imaging System
    Jeremy M Boss; Fiscal Year: 2010
    ..Technologies ranging from Southern and northern blotting, EMSA, DNA footprinting, multi-color immunoblotting, two-dimensional separations, to thin layer ..
  18. Effect of intracellular localization of miRNAs on miRNA-mediated gene regulation
    TERESA MARIA ABRAHAM; Fiscal Year: 2011
    ..Learning how miRNAs function normally will lend insight into disease progression and contribute to the development of new treatments. ..
  19. Gene Networks for Prostate Cancer Progression
    Leif Peterson; Fiscal Year: 2005
    ..Characterization of the FGFR1 and FGFR2 pathways in prostate cancer will contribute to the molecular understanding and early detection of patients that may experience rapid progression of metastases. ..
  20. MOLECULAR ANALYSIS OF FLOWERING GENES OF PHARBITIS NIL
    Anath Das; Fiscal Year: 1991
    ..thaliana genes by similar procedures. Induction of flowering is a critical process in angiosperm lifecycle. Understanding how photoperiod control this process will greatly broaden our knowledge on developmental processes in plants...
  21. OZONE INDUCED AIRWAY EPITHELIAL HYPERPROLIFERATION
    CHRISTOPHER MURLAS; Fiscal Year: 1999
    ..It is hoped this information will increase their understanding concerning the importance of cell NEP expression in modulating airway epithelial mitogenesis, especially as it may relate to airway disease due to repeated ozone exposure. ..
  22. EFFECT OF AGING ON INTERORGAN GLUTATHIONE HOMEOSTASIS
    Murad Ookhtens; Fiscal Year: 1999
    ..We will examine for changes in synthetic capacity of GSH from CYSH, using cytosol and freshly-isolated hepatocytes. Mitochondrial GSH pool sizes and rates of transport will be studied by tracer-kinetic methods. ..
  23. The Biology of Influenza A Virus-Generated Small RNAs
    Benjamin R tenOever; Fiscal Year: 2013
    ..This proposal focuses on the study of a novel small RNA component of the virus in an effort to reveal novel molecular targets for anti-viral design. ..
  24. Early agr Activation is a Key Pathogenic Signature in Persistent MRSA Bacteremia
    Yan Q Xiong; Fiscal Year: 2013
    ..Our long-term goal is to identify unique PB signatures for development of rapid diagnostic means and novel antimicrobial strategies against MRSA infections, such as PB. ..
  25. UROTHELIAL CELL ACTIVATION IN INTERSTITIAL CYSTITIS
    Monica Liebert; Fiscal Year: 1993
    ..production of cytokines will be evaluated by the messenger RNA (mRNA) polymerase chain reaction (PCR) and by Northern blotting. PCR, will be used because it will allow the evaluation of multiple cytokines from very small samples...
  26. STEROID HORMONES AND NEURONAL REGENERATION
    Kathryn Jones; Fiscal Year: 2001
    ..AR binding assays will also be done to determine if E binds to AR in the facial nucleus. ..
  27. Microfluidic mRNA Integrity Assay
    Javier Farinas; Fiscal Year: 2013
    ..Such an assay is expected to give more accurate results when mRNA levels are measured using qPCR, microarrays or RNAseq. ..
  28. CELLULAR CONSEQUENCES OF DEFECTIVE IIB AND IIIA GENES
    Paul Bray; Fiscal Year: 1993
    ..Defects in coding sequences will be sought by analysis of platelet RNA using Northern blotting and the polymerase chain reaction (PCR)...
  29. CASPASES IN RENAL TUBULAR EPITHELIAL CELL INJURY
    GUR KAUSHAL; Fiscal Year: 2000
    ..These studies will provide new insights into our understanding of the cellular and molecular mechanisms of ischemic renal injury as well as other forms of acute renal failure. ..
  30. MONOCYTE CHEMOATTRACTANT PROTEIN 1 IN THE CORPUS LUTEUM
    PAUL KEYES; Fiscal Year: 1999
    ....
  31. REGULATION OF SODIUM CHANNEL EXPRESSION
    ROBERT MAUE; Fiscal Year: 2000
    ....
  32. REGULATION OF TISSUE FACTOR PATHWAY INHIBITOR EXPRESSION
    MADHU BAJAJ; Fiscal Year: 2000
    ..Using Northern blotting analysis and TEPI assays, the applicant had previously demonstrated that endothelium is the primary ..
  33. COCAINE AND CATECHOLAMINE EFFECTS ON T LYMPHOCYTES
    Thomas Klein; Fiscal Year: 1993
    ..Also, drug effects on the upregulation of IL-2 gene expression will be determined by Northern blotting. Co-cultivation studies with various monokines will also be instituted to determine whether these accessory ..
  34. CALCITONIN RECEPTOR GENE EXPRESSION
    Steven Goldring; Fiscal Year: 2002
    ..g. kidney). This will define the structural basis of CTR gene regulation and permit identification of potentially unique factors that regulate the CTR. ..
  35. STUDIES OF MEGAKARYOCYTOPOIESIS
    Samuel Burstein; Fiscal Year: 1991
    ..directly at the transcriptional level by measuring steady-state splenic IL-3 messenger RNA by dot and Northern blotting, and/or solution hybridization with RNA probes for IL-3...
  36. MOLECULAR MECHANISMS OF CELL COMMUNICATION IN ATHEROGENE
    Peter Davies; Fiscal Year: 1993
    ..These studies will address a poorly understood mechanism of vascular cell communication at the tissue, cell and molecular level as a function of atherogenesis...
  37. MOLECULAR MECHANISMS CELL COMMUNICATIONS/ATHEROGENESIS
    Peter Davies; Fiscal Year: 2002
    ..These studies will address a poorly understood mechanism of vascular cell communication at the tissue, cell and molecular level as a function of atherogenesis. ..
  38. CYTOKINES AND AIDS DEMENTIA COMPLEX
    Iain Campbell; Fiscal Year: 2000
    ....
  39. GLUCOCORTICOIDS AND MATRIX PROTEINS AT PARTURITION
    Seth Guller; Fiscal Year: 1999
    ..collagens I, III, and IV in cytotrophoblasts and amnion cells will be evaluated using ELISA, Western and Northern blotting and immunoprecipitation procedures...
  40. SECOND MESSENGERS IN ASTROCYTES AND PRECURSOR CELLS
    Bridget Shafit Zagardo; Fiscal Year: 1991
    ..These studies will provide insight into the molecular mechanisms by which extracellular stimuli, such as cell-cell contact and growth factors, induce commitment of nervous system precursors to particular lineages...
  41. PINEAL GAP JUNCTION--CHARACTERIZATION AND REGULATION
    JUAN SAEZ; Fiscal Year: 1992
    ..The pineal gap junction may also serve as a model of electrical synapses in the central nervous system...
  42. ENDOCRINE CONTROL OF FETAL CARDIOVASCULAR DYNAMICS
    CECILIA CHEUNG; Fiscal Year: 2000
    ..This information will provide the scientific basis for diagnosis and treatment of fetal cardiovascular and fluid disorders associated with stress such as hypoxia. ..
  43. A Plasmid-Based miRNA Sensor Library for Use in Mice
    David Lewis; Fiscal Year: 2006
    ..Techniques for monitoring messenger RNA expression such as Northern blotting and microarray analysis have been adapted to monitor miRNA expression...
  44. GROWTH RETARDATION IN CHRONIC RENAL FAILURE
    Robert Mak; Fiscal Year: 2001
    ....
  45. ASYMMETRICALLY EXPRESSED GENES IN DEVELOPING CEREBRUM
    Daniel Geschwind; Fiscal Year: 2003
    ..Further secondary screening using RT-PCR or Northern blotting has confirmed the differential expression of 2of these genes: one that is enriched on the right and the ..
  46. ABI PRISM 7700 SEQUENCE DETECTION SYSTEM
    Derry Roopenian; Fiscal Year: 1999
    ..Funds are requested to purchase the ABI PRISM 7700 Sequence Detection System, necessary computer hardware, operating/data analysis software and uninterruptible power supply. ..
  47. MOLECULAR GENETICS OF HUMAN LEUKEMIA AND LYMPHOMA
    TIMOTHY MEEKER; Fiscal Year: 1991
    ..the breakpoints noted above from genomic libraries and the identification of an adjacent transcribed gene by Northern blotting. These transcribed genes will then be cloned from cDNA libraries...
  48. ETIOLOGY OF ESTROGEN INDUCED PROLACTIN TUMORS
    Richard Weiner; Fiscal Year: 1991
    ..These studies will increase our understanding of the mechanisms and cellular sites of action of E2 in tumor formation. Studies with 16K PRL could lead to the development of a new class of angiolytic drugs for treatment of cancer...
  49. ANALYSIS OF 8P LOSS IN HUMAN PROSTATE CANCER
    Jill Macoska; Fiscal Year: 2004
    ..This work should identify TSGs critical for prostate tumorigenesis mapping to proximal 8p and provide a basis for understanding their biological functions and their role(s) in malignant transformation and progression. ..
  50. Attenuation of mucous cell metaplasia by Rad
    Mary Chang; Fiscal Year: 2006
    ..In summary, we have proposed a comprehensive approach utilizing combined in vitro, cell, and in vivo methods to test our hypothesis. ..
  51. TISSUE FACTOR MESSENGER RNA, ANTIGEN, AND ACTIVITY
    Mary Todd; Fiscal Year: 1993
    ....
  52. Trapping Cumulus Products for Contraceptive Targeting
    John Eppig; Fiscal Year: 2006
    ..abstract_text> ..
  53. AFFINITY LABELING OF THE ADIPOCYTE HEXOSE CARRIER
    James May; Fiscal Year: 1993
    ....
  54. PE Biosystems TaqMan Quantitative PCR Model 7700
    Richard Ulevitch; Fiscal Year: 2001
    ..For example, mRNA levels for a gene of interest can be determined by northern blotting. This method is very time-consuming, due to running and blotting gels, hybridization, and washing steps and ..
  55. NUTRITIONAL REGULATION OF SELENOENZYMES AND PROTEINS
    ROGER SUNDE; Fiscal Year: 1992
    ..This knowledge may lead to a better understanding of the nutritional biochemistry of Se and it may increase our understanding of selenium's full role in animal and human health...
  56. INFLAMMATORY AND STEROID MODULATION OF AIRWAY B-RECEPTOR
    Peter Barnes; Fiscal Year: 1992
    ....
  57. TRANSCRIPTIONAL REGULATION OF GNRH GENE EXPRESSION
    DIANE STAFFORD; Fiscal Year: 2003
    ..The utilization of multiple techniques for the identification and characterization of novel proteins important in gene regulation will allow me to develop the skills necessary to become an independent investigator. ..
  58. Novel Method for Micro RNA Amplification
    Donald Rao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  59. Role of Non-Coding RNAs in P. aeruginosa Biofilm Development
    Michael Franklin; Fiscal Year: 2009
    ..have demonstrated expression of many of these predicted ncRNAs by using custom-designed microarrays and by Northern blotting experiments. Our present goals are to: (I) Map the genome position of the P...
  60. METASTASIS SUPPRESSOR GENE IN HUMAN CUTANEOUS MELANOMA
    DANNY WELCH; Fiscal Year: 1999
    ..Third, they will identify critical KiSS-1 protein/macromolecule interactions using plaque library screening an immune co-precipitation experiments. ..
  61. MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATION
    Charles H Lang; Fiscal Year: 2010
    ..Overall, the research design will establish the mechanism by which myocardial protein synthesis is reduced in response to alcohol abuse. ..
  62. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2004
    ..abstract_text> ..