northern blotting

Summary

Summary: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

Top Publications

  1. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
  2. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
  3. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
  4. ncbi MicroRNAs modulate hematopoietic lineage differentiation
    Chang Zheng Chen
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 303:83-6. 2004
  5. pmc Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells
    Xuezhong Cai
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:5570-5. 2005
  6. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
  7. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
  8. ncbi An integrated stress response regulates amino acid metabolism and resistance to oxidative stress
    Heather P Harding
    Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 11:619-33. 2003
  9. pmc Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia
    George Adrian Calin
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:15524-9. 2002
  10. pmc A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
    Tao Zhao
    National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Genes Dev 21:1190-203. 2007

Research Grants

Detail Information

Publications315 found, 100 shown here

  1. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
    ..This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated...
  2. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
    ..The abundance of these tiny RNAs, their expression patterns, and their evolutionary conservation imply that, as a class, miRNAs have broad regulatory functions in animals...
  3. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
    ....
  4. ncbi MicroRNAs modulate hematopoietic lineage differentiation
    Chang Zheng Chen
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 303:83-6. 2004
    ..Our results indicate that microRNAs are components of the molecular circuitry that controls mouse hematopoiesis and suggest that other microRNAs have similar regulatory roles during other facets of vertebrate development...
  5. pmc Kaposi's sarcoma-associated herpesvirus expresses an array of viral microRNAs in latently infected cells
    Xuezhong Cai
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:5570-5. 2005
    ..We hypothesize that these viral miRNAs play a critical role in the establishment and/or maintenance of KSHV latent infection in vivo and, hence, in KSHV-induced oncogenesis...
  6. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
  7. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
    ..elegans larval development, and three of them have apparent homologs in mammals and/or insects. Small noncoding RNAs of the miRNA class appear to be numerous and diverse...
  8. ncbi An integrated stress response regulates amino acid metabolism and resistance to oxidative stress
    Heather P Harding
    Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 11:619-33. 2003
    ..A signaling pathway initiated by eIF2alpha phosphorylation protects cells against metabolic consequences of ER oxidation by promoting the linked processes of amino acid sufficiency and resistance to oxidative stress...
  9. pmc Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia
    George Adrian Calin
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:15524-9. 2002
    ..Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases...
  10. pmc A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii
    Tao Zhao
    National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
    Genes Dev 21:1190-203. 2007
    ..Our findings suggest that the miRNA pathway and some siRNA pathways are ancient mechanisms of gene regulation that evolved prior to the emergence of multicellularity...
  11. ncbi Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA
    Ranhui Duan
    Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Hum Mol Genet 16:1124-31. 2007
    ..Thus, our study reveals an additional structural requirement for pri-miRNA processing and emphasizes the importance of identifying new miRNA SNPs and their contributions to miRNA biogenesis and human genetic disease...
  12. pmc Role of Leishmania (Leishmania) chagasi amastigote cysteine protease in intracellular parasite survival: studies by gene disruption and antisense mRNA inhibition
    Vasanthakrishna Mundodi
    Department of Biological Sciences, University of Calgary, Calgary AB T2N1N4, Canada
    BMC Mol Biol 6:3. 2005
    ..chagasi. Here, we have further characterized this cysteine protease gene and demonstrated its role during infection and survival of Leishmania (L.) chagasi within the U937 macrophage cells...
  13. doi MicroRNA detection by northern blotting using locked nucleic acid probes
    Eva Várallyay
    Agricultural Biotechnology Center, Plant Virology Group, Szent Gyorgyi Albert ut 4, Gödöllõ H 2100, Hungary
    Nat Protoc 3:190-6. 2008
    ..for miRNA northern blot analysis, which includes RNA extraction, polyacrylamide gel electrophoresis and northern blotting, and the hybridization and detection of locked nucleic acid (LNA)-modified oligonucleotide probes...
  14. pmc MicroRNA-binding viral protein interferes with Arabidopsis development
    Padmanabhan Chellappan
    International Laboratory for Tropical Agricultural Biotechnology, Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA
    Proc Natl Acad Sci U S A 102:10381-6. 2005
    ....
  15. ncbi NDRG1 is necessary for p53-dependent apoptosis
    Susanne Stein
    Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Biol Chem 279:48930-40. 2004
    ..This report further supports the notion that p53 controls a network of genes that are required for its apoptotic function...
  16. pmc The G-protein alpha-subunit GasC plays a major role in germination in the dimorphic fungus Penicillium marneffei
    Sophie Zuber
    Department of Genetics, University of Melbourne, 3010 Victoria, Australia
    Genetics 164:487-99. 2003
    ..In contrast to the S. cerevisiae ortholog Gpa2, our data indicate that GasC is not involved in carbon or nitrogen source sensing and plays no major role in either hyphal or yeast growth or in the switch between these two forms...
  17. ncbi Autophagy is required for maintenance of amino acid levels and protein synthesis under nitrogen starvation
    Jun Onodera
    Division of Molecular Cell Biology, National Institute for Basic Biology, and Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies, Nishigonaka 38, Myodaiji cho, Okazaki 444 8585, Japan
    J Biol Chem 280:31582-6. 2005
    ..Autophagy-defective cells fail to maintain physiologic amino acid levels, and their inability to synthesize new proteins may explain most phenotypes associated with autophagy mutants at least partly...
  18. pmc A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Genes Dev 18:2491-505. 2004
    ..These findings suggest that nutrient signals set the critical cell-size threshold via Sfp1 and Sch9-mediated control of ribosome biosynthetic rates...
  19. ncbi Vascular endothelial growth factor is a secreted angiogenic mitogen
    D W Leung
    Department of Molecular Biology, Genetech, South San Francisco, CA 94080
    Science 246:1306-9. 1989
    ..Human 293 cells transfected with an expression vector containing a bovine or human VEGF cDNA insert secrete an endothelial cell mitogen that behaves like native VEGF...
  20. ncbi A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper
    Sun Hwa Ha
    National Institute of Agricultural Biotechnology, RDA, Suwon 441 707, Republic of Korea
    J Exp Bot 58:3135-44. 2007
    ....
  21. ncbi The chemotactic receptor for human C5a anaphylatoxin
    N P Gerard
    Ina Sue Perlmutter Research Laboratory, Childrens Hospital, Boston, Massachusetts
    Nature 349:614-7. 1991
    ..The deduced amino-acid sequence of the receptor reveals the expected motifs befitting its interaction with cellular GTP-binding proteins...
  22. ncbi Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs
    N Porchet
    Unité 16 INSERM, Lille, France
    Biochem Biophys Res Commun 175:414-22. 1991
    ..This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3...
  23. ncbi The noncoding RNA taurine upregulated gene 1 is required for differentiation of the murine retina
    T L Young
    Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 15:501-12. 2005
    ..However, of the thousands of ncRNAs identified, the functions of relatively few have been established...
  24. ncbi The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis
    D Finley
    Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115
    Nature 338:394-401. 1989
    ..These results suggest a novel 'chaperone' function for ubiquitin, in which its covalent association with other proteins promotes the formation of specific cellular structures...
  25. pmc CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus
    A Rowley
    Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada
    Mol Cell Biol 11:5718-26. 1991
    ....
  26. ncbi TGF-beta 1 binding protein: a component of the large latent complex of TGF-beta 1 with multiple repeat sequences
    T Kanzaki
    Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden
    Cell 61:1051-61. 1990
    ..TGF-beta 1-BP was found not to bind and inactive TGF-beta 1; its role in the latent complex is discussed...
  27. ncbi Discovery in silico and characterization in vitro of novel genes exclusively expressed in the mouse epididymis
    Jenni Penttinen
    Department of Physiology, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
    Mol Endocrinol 17:2138-51. 2003
    ..However, the expression of AV381130 was not detected until d 30 after birth, indicating a close connection between gene expression and puberty...
  28. pmc Characterization of Dicer-deficient murine embryonic stem cells
    Elizabeth P Murchison
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 102:12135-40. 2005
    ..These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells...
  29. pmc A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    Adam Grundhoff
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
    RNA 12:733-50. 2006
    ..The advantages and limitations of the approach are discussed...
  30. pmc Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response
    Phoebe D Lu
    Department of Cell Biology, School of Medicine, New York University, New York, NY 10016, USA
    J Cell Biol 167:27-33. 2004
    ..These features are common to regulated translation of GCN4 in yeast. The metazoan ISR thus resembles the yeast general control response both in its target genes and its mechanistic details...
  31. ncbi Engineering plants with increased levels of the antioxidant chlorogenic acid
    Ricarda Niggeweg
    John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
    Nat Biotechnol 22:746-54. 2004
    ..Tomatoes with elevated CGA levels could be used in foods with specific benefits for human health...
  32. ncbi Repression of farnesoid X receptor during the acute phase response
    Min Sun Kim
    Department of Medicine, University of California San Francisco, Metabolism Section, Medical Service, Department of Veterans Affairs Medical Center, San Francisco, California 94121, USA
    J Biol Chem 278:8988-95. 2003
    ....
  33. ncbi Oogenesin is a novel mouse protein expressed in oocytes and early cleavage-stage embryos
    Naojiro Minami
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Biol Reprod 69:1736-42. 2003
    ..These results suggest that the gene has some roles in zygotic transcription of early preimplantation embryos as well as folliculogenesis and oogenesis in the mouse...
  34. pmc A truncated laminin chain homologous to the B2 chain: structure, spatial expression, and chromosomal assignment
    P Kallunki
    Department of Biochemistry, University of Oulu, Finland
    J Cell Biol 119:679-93. 1992
    ..By in situ hybridization expression of the B2t chain was localized to specific epithelial cells in skin, lung, and kidney as opposed to a general epithelial and endothelial cell expression of the laminin B2 chain in the same tissues...
  35. ncbi The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae
    M Tucker
    Department of Molecular and Cellular Biology and, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Cell 104:377-86. 2001
    ..Interestingly, because Ccr4p and Caf1p interact with transcription factors, these results suggest an unexpected link between mRNA synthesis and turnover...
  36. ncbi Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues
    S Lenzen
    Institute of Clinical Biochemistry, Hannover Medical School, Germany
    Free Radic Biol Med 20:463-6. 1996
    ....
  37. pmc cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes
    G Manfioletti
    Universita di Trieste, Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Italy
    Nucleic Acids Res 19:6793-7. 1991
    ..Analysis of mRNA levels demonstrate that the HMGI-C gene is not expressed in a variety of mouse tissues but is expressed in Lewis lung carcinoma cells...
  38. ncbi Cloning and functional expression of a human kidney Na+:HCO3- cotransporter
    C E Burnham
    Department of Internal Medicine, University of Cincinnati School of Medicine, Cincinnati, Ohio 45267 0585, USA
    J Biol Chem 272:19111-4. 1997
    ..6-kilobase mRNA is highly expressed in kidney and pancreas, with detectable levels in brain. Functional studies in transiently transfected HEK-293 cells demonstrate that the cloned transporter mediates Na+:HCO3- cotransport...
  39. ncbi Prostate-specific and androgen-dependent expression of a novel homeobox gene
    C J Bieberich
    Department of Virology, Jerome H Holland Laboratory, Rockville, Maryland 20855, USA
    J Biol Chem 271:31779-82. 1996
    ..1 protein, which likely functions as a transcription factor, plays a prominent role both in the initiation of prostate development and in the maintenance of the differentiated state of prostatic epithelial cells...
  40. ncbi Laminin alpha 2 is a component of brain capillary basement membrane: reduced expression in dystrophic dy mice
    M Jucker
    Molecular Neurobiology Unit, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Neuroscience 71:1153-61. 1996
    ..This observation might be explained by the fact that the cerebral capillary basement membrane expresses both alpha chains and therefore exhibits structural redundancy...
  41. ncbi Identification of additional genes belonging to the LexA regulon in Escherichia coli
    A R Fernandez de Henestrosa
    Section on DNA Replication, Repair and Mutagenesis, Building 6, Room 1A13, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2725, USA
    Mol Microbiol 35:1560-72. 2000
    ..These experiments have allowed us to identify seven new LexA-regulated genes, thus bringing the present number of genes in the E. coli LexA regulon to 31. The potential function of each newly identified LexA-regulated gene is discussed...
  42. ncbi Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter
    Olexander Korchynskyi
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 277:4883-91. 2002
    ..Our results provide important new insights into how the BMP/Smad pathway can specifically activate target genes...
  43. ncbi Suprabasal expression of a dominant-negative RXR alpha mutant in transgenic mouse epidermis impairs regulation of gene transcription and basal keratinocyte proliferation by RAR-selective retinoids
    X Feng
    Department of Dermatology, University of Michigan, Ann Arbor 48109 0314, USA
    Genes Dev 11:59-71. 1997
    ....
  44. ncbi Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans
    C Reynet
    Research Division, Joslin Diabetes Center, Boston, MA
    Science 262:1441-4. 1993
    ..Messenger ribonucleic acid of Rad was expressed primarily in skeletal and cardiac muscle and was increased an average of 8.6-fold in the muscle of Type II diabetics as compared to normal individuals...
  45. ncbi P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction
    Kristin D Kasschau
    Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
    Dev Cell 4:205-17. 2003
    ..The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway...
  46. ncbi 4'-phosphopantetheinyl transferase-encoding npgA is essential for siderophore biosynthesis in Aspergillus nidulans
    Harald Oberegger
    Department of Molecular Biology, University of Innsbruck, Peter Mayr Strasse 4b, 6020 Innsbruck, Austria
    Curr Genet 44:211-5. 2003
    ....
  47. pmc A microRNA array reveals extensive regulation of microRNAs during brain development
    Anna M Krichevsky
    Center for Neurologic Diseases, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    RNA 9:1274-81. 2003
    ..Creating clusters of coexpressed miRNAs will contribute to understanding their regulation, functions, and discovery of mRNA targets...
  48. ncbi Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development
    Savraj S Grewal
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Nat Cell Biol 7:295-302. 2005
    ..In addition, the growth effects of dMyc in larval wing imaginal discs require de novo rRNA synthesis. We suggest that during animal development, the control of rRNA synthesis and ribosome biogenesis is an essential Myc function...
  49. pmc A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis
    Rebecca L Brown
    Cooperative Research Centre for Tropical Plant Protection, Brisbane, Queensland 4072, Australia
    Plant Physiol 132:1020-32. 2003
    ....
  50. pmc Nuclear export and cytoplasmic processing of precursors to the 40S ribosomal subunits in mammalian cells
    Jacques Rouquette
    Laboratoire de Biologie Moléculaire des Eucaryotes and Institut d Exploration Fonctionnelle des Génomes, CNRS and Université Paul Sabatier, Toulouse Cedex, France
    EMBO J 24:2862-72. 2005
    ..It also allows new strategies for the study of preribosomal transport in mammalian cells...
  51. ncbi Induction and expression of betaig-h3 in pancreatic cancer cells
    Dominik Schneider
    Department of Visceral and Transplantation Surgery, University of Bern, Bern, Switzerland
    Biochim Biophys Acta 1588:1-6. 2002
    ....
  52. ncbi The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(-/-) mice and results in a mouse with spinal muscular atrophy
    U R Monani
    Departments of Neurology, College of Medicine, Ohio State University, Columbus, OH 43210, USA
    Hum Mol Genet 9:333-9. 2000
    ..These results show that SMA is caused by insufficient SMN production by the SMN2 gene and that increased expression of the SMN2 gene may provide a strategy for treating SMA patients...
  53. ncbi A mammalian gene with introns instead of exons generating stable RNA products
    K T Tycowski
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 379:464-6. 1996
    ..Thus, the introns rather than the exons specify the functional products of UHG...
  54. pmc Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot
    Gurman Singh Pall
    Division of Cancer Sciences and Molecular Pathology, Glasgow University, Western Infirmary, Dumbarton Road, Glasgow, G11 6NT, UK
    Nucleic Acids Res 35:e60. 2007
    ..However, increasing the dose of UV reduced the detection of miRNA by hybridization in northern blotting experiments...
  55. pmc Complex molecular architecture of beet yellows virus particles
    Valera V Peremyslov
    Department of Botany and Plant Pathology and Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331, USA
    Proc Natl Acad Sci U S A 101:5030-5. 2004
    ..The unexpectedly complex structure of closterovirus virions has important mechanistic and functional implications that may also apply to other virus families...
  56. ncbi Clinical significance of secreted protein acidic and rich in cystein in esophageal carcinoma and its relation to carcinoma progression
    Keishi Yamashita
    Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan
    Cancer 97:2412-9. 2003
    ..The goal of this study was to show the clinical relevance of SPARC and its relation to MMP-2 expression in esophageal carcinoma patients...
  57. ncbi The epithelin precursor encodes two proteins with opposing activities on epithelial cell growth
    G D Plowman
    Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121
    J Biol Chem 267:13073-8. 1992
    ..The broad expression profile of epithelin transcripts, along with the opposing activities of the two mature protein products, implicates these factors as natural mediators of epithelial homeostasis...
  58. pmc Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains
    Christophe Pichon
    Biochimie Pharmaceutique, Universite de Rennes I, Unité Propre de Recherche de l Enseignement Supérieur JE 2311, Inserm ESPRI, 2 Avenue du Professeur Leon Bernard, 35043 Rennes, France
    Proc Natl Acad Sci U S A 102:14249-54. 2005
    ..Some of these RNAs show large variations of expression among pathogenic strains, suggesting that they are involved in the regulation of staphylococcal virulence...
  59. pmc RNomics in Escherichia coli detects new sRNA species and indicates parallel transcriptional output in bacteria
    Jörg Vogel
    Institute of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, 75124 Uppsala, Sweden
    Nucleic Acids Res 31:6435-43. 2003
    ..The experimental characterization of sRNAs analyzed here suggests that the definition of an sRNA is more complex than previously assumed...
  60. ncbi Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7)
    L A Bobek
    Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214
    J Biol Chem 268:20563-9. 1993
    ..MG2 mRNA is about 2.5 kilobases long, and its expression appears to be species-, tissue-, and cell-specific. We propose to name this gene MUC7 in accordance with the mucin genes cloned to date named MUC1-MUC6...
  61. ncbi Stabilization of mRNA expression in whole blood samples
    Lynne Rainen
    PreAnalytiX CH c o Becton Dickinson, Franklin Lakes, NJ 07417, USA
    Clin Chem 48:1883-90. 2002
    ....
  62. pmc IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase
    Shyun Li
    Tularik, Inc, 2 Corporate Drive, South San Francisco, CA 94080, USA
    Proc Natl Acad Sci U S A 99:5567-72. 2002
    ....
  63. pmc Axin facilitates Smad3 activation in the transforming growth factor beta signaling pathway
    M Furuhashi
    Department of Biochemistry, The Japanese Foundation for Cancer Research JFCR Cancer Institute, Toshima ku, Tokyo 170 8455, Japan
    Mol Cell Biol 21:5132-41. 2001
    ..Axin may thus function as an adapter of Smad3, facilitating its activation by TGF-beta receptors for efficient TGF-beta signaling...
  64. ncbi Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis
    A Shiratori
    Cellular Physiology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
    Yeast 15:219-53. 1999
    ..This suggests that helicase-like genes may be involved in the biosynthesis of nucleic acids and proteins, and that the genes can be transcriptionally activated by heat shock to compensate for the repressed synthesis of mRNA and protein...
  65. pmc Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans
    Rosalind C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, New Hamphsire 03755, USA
    RNA 12:589-97. 2006
    ..elegans. Microarray expression profiling of RNAi-defective mutant worms further suggests diverse endogenous RNAi pathways for silencing different sets of genes...
  66. ncbi Cloning of the LamA3 gene encoding the alpha 3 chain of the adhesive ligand epiligrin. Expression in wound repair
    M C Ryan
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    J Biol Chem 269:22779-87. 1994
    ..In contrast, epiligrin expression was up-regulated in wound sites of human skin...
  67. ncbi Differential expression of the "B" subunit of the vacuolar H(+)-ATPase in bovine tissues
    K Puopolo
    Department of Physiology and Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111
    J Biol Chem 267:3696-706. 1992
    ..2-kb message can be detected. Northern blotting of RNA isolated from human fibroblast and human lung tumor cell lines reveals that three messages of ..
  68. pmc Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc
    K A Montzka
    Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven 06510
    Proc Natl Acad Sci U S A 85:8885-9. 1988
    ..Potential roles for the low-abundance snRNPs in aspects of pre-mRNA processing are discussed...
  69. ncbi An insertional mutation in the BTF3 transcription factor gene leads to an early postimplantation lethality in mice
    J M Deng
    Department of Molecular Genetics, University of Texas, M D Anderson Cancer Center, Houston 77030, USA
    Transgenic Res 4:264-9. 1995
    ..5. Thus, BTF3 is essential for postimplantation development. The isolation of the BTF3 sequences in this ROSA beta-geo insertion was facilitated by a relatively simple single lacZ primer reverse transcription PCR strategy...
  70. ncbi Novel small RNA-encoding genes in the intergenic regions of Escherichia coli
    L Argaman
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, 91120, Jerusalem, Israel
    Curr Biol 11:941-50. 2001
    ..Thus, in spite of the rising recognition that such RNAs may play key roles in bacterial physiology, many of the small RNAs known to date were discovered fortuitously...
  71. pmc Human eosinophil cationic protein. Molecular cloning of a cytotoxin and helminthotoxin with ribonuclease activity
    H F Rosenberg
    Division of Infectious Diseases, Harvard Medical School, Boston, Massachusetts
    J Exp Med 170:163-76. 1989
    ..Despite similarities in sequence and cellular localization, these results suggest that ECP and EDN are subject to different regulatory mechanisms...
  72. doi Northern blotting techniques for small RNAs
    Todd Blevins
    Pikaard Laboratory, Biology Department, Washington University, MO, USA
    Methods Mol Biol 631:87-107. 2010
    ..Finally, the usefulness of northern blotting in dissecting smRNA biogenesis is shown for the particular case of DNA virus infection.
  73. ncbi The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis
    Markus Landthaler
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Curr Biol 14:2162-7. 2004
    ..Our results suggest that DGCR8 and Drosha interact in human cells and reside in a functional pri-miRNA processing complex...
  74. ncbi Predisposition to lymphomagenesis in pim-1 transgenic mice: cooperation with c-myc and N-myc in murine leukemia virus-induced tumors
    M van Lohuizen
    Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam
    Cell 56:673-82. 1989
    ..In all these T cell lymphomas either c-myc or N-myc was activated by proviral insertion, suggesting strong cooperation between pim-1 and myc in lymphomagenesis...
  75. pmc An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
    Chang Gong Liu
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 101:9740-4. 2004
    ..The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states...
  76. ncbi A heat-sensitive TRP channel expressed in keratinocytes
    Andrea M Peier
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Science 296:2046-9. 2002
    ..TRPV3 is specifically expressed in keratinocytes; hence, skin cells are capable of detecting heat via molecules similar to those in heat-sensing neurons...
  77. ncbi Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development
    A Sugimoto
    Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan
    Genes Dev 5:1990-9. 1991
    ..These observations strongly suggest that Ste11 serves as a key transcription factor for sexual development...
  78. ncbi Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells
    Maria Sueli S Felipe
    Departamento de Biologia Celular, Universidade de Brasilia, 70910 900, Brasilia, DF, Brazil
    J Biol Chem 280:24706-14. 2005
    ..Novel P. brasiliensis genes have been identified, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets...
  79. ncbi Human MUC4 mucin cDNA and its variants in pancreatic carcinoma
    A Choudhury
    Department of Biochemistry and Molecular Biology, Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198 4525, USA
    J Biochem 128:233-43. 2000
    ..5 to 10.0 kb. MUC4 mRNA expression was higher in differentiated cell lines, with no detectable expression in poorly differentiated pancreatic tumor cell lines...
  80. ncbi Identification and characterization of genes involved in the carcinogenesis of human squamous cell cervical carcinoma
    Qing Cheng
    Laboratory of Gene Structure and Expression, Division of Cellular and Molecular Research, National Cancer Centre, Singapore
    Int J Cancer 98:419-26. 2002
    ..The ribosomal protein S12 and the NADH dehydrogenase 4 genes may therefore be potentially useful as early pre-transformation diagnostic markers for human cervical cancer...
  81. ncbi The murine polycomb group protein Eed is required for global histone H3 lysine-27 methylation
    Nathan D Montgomery
    Department of Genetics, University of North Carolina at Chapel Hill, Call Box 7264, Chapel Hill, North Carolina 27599, USA
    Curr Biol 15:942-7. 2005
    ..These results provide a functionally important distinction between PRC2 complex components and implicate Eed in PRC2-independent histone methylation...
  82. ncbi IRAK4 kinase activity is redundant for interleukin-1 (IL-1) receptor-associated kinase phosphorylation and IL-1 responsiveness
    Jinzhong Qin
    Department of Immunology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 279:26748-53. 2004
    ..Moreover, consistent with the fact that IRAK4 is a necessary component of the IL-1 pathway, we found that IRAK4 was required for the efficient recruitment of IRAK to the IL-1 receptor complex...
  83. ncbi Neuronal distribution and functional characterization of the calcium channel alpha2delta-2 subunit
    M Hobom
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Germany
    Eur J Neurosci 12:1217-26. 2000
    ....
  84. ncbi Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4
    R Xu
    State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, 200433, People s Republic of China
    Genomics 62:537-9. 1999
    ..Northern blot analysis revealed that the mRNA of ADAM29 is highly expressed in the testis. By radiation hybrid panel mapping, the ADAM29 gene was assigned to human chromosome 4q34.2-qter...
  85. ncbi Chromosome transfer activates and delineates a locus control region for perforin
    Matthew E Pipkin
    Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Immunity 26:29-41. 2007
    ..Thus, LCR function is central for regulating the developmental and activation-specific PRF1 promoter activity characteristic of NK cells and CTL...
  86. ncbi Molecular and developmental characterization of novel cDNAs of the myelin-associated/oligodendrocytic basic protein
    A Holz
    Brain Research Institute, University of Zurich, Switzerland
    J Neurosci 16:467-77. 1996
    ..The late developmental expression of the MOBP gene suggests that the MOBP proteins act at the late steps of myelin formation, possibly in myelin compaction and in the maintenance of the myelin sheath...
  87. ncbi Expression of monocyte chemoattractant protein-1 and distribution of immune cell populations in the bovine corpus luteum throughout the estrous cycle
    David H Townson
    Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, New Hampshire 03824 3590, USA
    Biol Reprod 66:361-6. 2002
    ..These results also raise a provocative issue, however, concerning the recruitment of immune cells several days in advance of the onset of luteal regression...
  88. pmc Impact of aeration and heme-activated respiration on Lactococcus lactis gene expression: identification of a heme-responsive operon
    Martin Bastian Pedersen
    Department of Physiology, Chr Hansen A S, DK 2970 Hørsholm, Denmark
    J Bacteriol 190:4903-11. 2008
    ..We give experimental evidence that the ygfCBA genes are involved in heme homeostasis...
  89. ncbi A novel complementary deoxyribonucleic acid is abundantly and specifically expressed in the uterus during pregnancy
    J Kasik
    MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH 44109, USA
    Am J Obstet Gynecol 176:452-6. 1997
    ..Our purpose was to identify novel genes expressed by the uterus during late pregnancy...
  90. ncbi Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq
    Hiroshi Kawamoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 61:1013-22. 2006
    ..We also showed in vitro that SgrS forms a stable duplex with the ptsG mRNA, and that Hfq markedly facilitates the rate of duplex formation...
  91. pmc MicroRNA-guided processing impairs Plum pox virus replication, but the virus readily evolves to escape this silencing mechanism
    Carmen Simón-Mateo
    Centro Nacional de Biotecnologia CSIC, Campus Universidad Autonoma de Madrid, Spain
    J Virol 80:2429-36. 2006
    ..Furthermore, we show that virus chimeras readily escape RNA silencing interference through mutations within the miRNA target sequence, which mainly affected nucleotides matching the 5'-terminal region of the miRNA...
  92. ncbi Cloning and characterisation of a cysteine proteinase gene expressed in amastigotes of Leishmania (L.) amazonensis
    Fernanda Lasakosvitsch
    Department of Microbiology, Immunology and Parasitology, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Rua Botucatu, 862, 6o andar, Brazil
    Int J Parasitol 33:445-54. 2003
    ....
  93. ncbi Thyroid hormone activation in human vascular smooth muscle cells: expression of type II iodothyronine deiodinase
    H Mizuma
    First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan
    Circ Res 88:313-8. 2001
    ..The present results suggest a previously unrecognized role of local T(3) production by DII in the pathophysiology of human vascular smooth muscle cells...
  94. ncbi Molecular cloning and characterization of the mouse and human TUSP gene, a novel member of the tubby superfamily
    Q Z Li
    Department of Pathology, Immunology and Laboratory Medicine, Center for Mammalian Genetics and Diabetes Center of Excellence, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Gene 273:275-84. 2001
    ..b>Northern blotting analysis showed that the major transcript with the complete coding sequence is expressed mainly in the brain,..
  95. pmc Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters
    M S Li
    Department of Cellular and Molecular Science, St George s Hospital Medical School, University of London, U K
    Biochem J 305:921-7. 1995
    ..b>Northern blotting and reverse-transcription PCR analysis showed that the 1.0 kb mRNA was present at higher levels than the 1...
  96. ncbi Retinoic acid receptor beta2 inhibition of metastasis in mouse mammary gland xenografts
    Piper M Treuting
    Department of Pathology, University of Washington, Seattle 98195, USA
    Breast Cancer Res Treat 72:79-88. 2002
    ..000001). Our findings suggest that RARbeta2 may play a role in inhibiting the metastatic cascade in a mouse mammary gland xenograft tumor model and is a potential candidate for therapeutic intervention in human breast cancer...
  97. ncbi Mitochondrial proteins that regulate apoptosis and necrosis are induced in mouse fatty liver
    A Rashid
    Department of Pathology, Johns Hopkins University, Baltimore, MD, USA
    Hepatology 29:1131-8. 1999
    ..However, if UCP2 induction reduces the efficiency of adenosine triphosphate (ATP) synthesis, this initially harmless response might enhance the vulnerability of hepatocytes to necrosis...
  98. pmc Comparative expression of fibroblast growth factor mRNAs in benign and malignant breast disease
    S Y Anandappa
    Department of Biochemistry, University of Liverpool, UK
    Br J Cancer 69:772-6. 1994
    ..The resultant production of basic fibroblast growth factor by breast cancer cells within some tumours may contribute to their development...
  99. pmc An X chromosome-linked gene encoding a protein with characteristics of a rhoGAP predominantly expressed in hematopoietic cells
    C Tribioli
    Istituto di Genetica Biochimica ed Evoluzionistica, Consiglio Nazionale delle Ricerche, Pavia, Italy
    Proc Natl Acad Sci U S A 93:695-9. 1996
    ..Its localization in a narrow cytoplasmic region just below the plasma membrane and its inhibitory effect on stress fiber organization indicate that p115 may down regulate rho-like GTPases in hematopoietic cells...
  100. ncbi Microarray analysis reveals previously unknown changes in Toxoplasma gondii-infected human cells
    I J Blader
    Department of Microbiology and Immunology, Stanford University, Stanford, California 94305-5124, USA
    J Biol Chem 276:24223-31. 2001
    ..These results reveal several previously unknown effects on the host cell and lay the foundation for detailed analysis of their role in the host-pathogen interaction...
  101. ncbi Differential gene expression by Paracoccidioides brasiliensis in host interaction conditions: representational difference analysis identifies candidate genes associated with fungal pathogenesis
    Alexandre Melo Bailão
    Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade Federal de Goias, 74001 970 Goiania, Goias, Brazil
    Microbes Infect 8:2686-97. 2006
    ..This work can facilitate functional studies of novel regulated genes that may be important for the survival and growth strategies of P. brasiliensis in humans...

Research Grants66

  1. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2006
    ..RT-PCR (for screening) and northern blotting (for quantitation) will analyze Gene expression...
  2. Cardiac Physiology and Imaging Core for Mouse in Vivo
    Kirk Peterson; Fiscal Year: 2009
    ..Chen) will access the personnel and resources of Core B in order to analyze the in vivo physiological and morphological characteristics of the mouse mutant intended to recapitulate Naxos disease, a recessive form of 262 ..
  3. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2003
    ..RT-PCR (for screening) and northern blotting (for quantitation) will analyze Gene expression...
  4. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2002
    ..RT-PCR (for screening) and northern blotting (for quantitation) will analyze Gene expression...
  5. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2004
    ..RT-PCR (for screening) and northern blotting (for quantitation) will analyze Gene expression...
  6. DNA methylation in aging, race and prostate cancer
    Rajvir Dahiya; Fiscal Year: 2005
    ..RT-PCR (for screening) and northern blotting (for quantitation) will analyze Gene expression...
  7. Gene Networks for Prostate Cancer Progression
    Leif Peterson; Fiscal Year: 2003
    ..Characterization of the FGFR1 and FGFR2 pathways in prostate cancer will contribute to the molecular understanding and early detection of patients that may experience rapid progression of metastases. ..
  8. Gene Networks for Prostate Cancer Progression
    Leif Peterson; Fiscal Year: 2004
    ..Characterization of the FGFR1 and FGFR2 pathways in prostate cancer will contribute to the molecular understanding and early detection of patients that may experience rapid progression of metastases. ..
  9. Gene Networks for Prostate Cancer Progression
    Leif Peterson; Fiscal Year: 2005
    ..Characterization of the FGFR1 and FGFR2 pathways in prostate cancer will contribute to the molecular understanding and early detection of patients that may experience rapid progression of metastases. ..
  10. OZONE INDUCED AIRWAY EPITHELIAL HYPERPROLIFERATION
    CHRISTOPHER MURLAS; Fiscal Year: 1999
    ..It is hoped this information will increase their understanding concerning the importance of cell NEP expression in modulating airway epithelial mitogenesis, especially as it may relate to airway disease due to repeated ozone exposure. ..
  11. MOLECULAR ANALYSIS OF FLOWERING GENES OF PHARBITIS NIL
    Anath Das; Fiscal Year: 1990
    ..thaliana genes by similar procedures. Induction of flowering is a critical process in angiosperm lifecycle. Understanding how photoperiod control this process will greatly broaden our knowledge on developmental processes in plants...
  12. EFFECT OF AGING ON INTERORGAN GLUTATHIONE HOMEOSTASIS
    Murad Ookhtens; Fiscal Year: 1999
    ..We will examine for changes in synthetic capacity of GSH from CYSH, using cytosol and freshly-isolated hepatocytes. Mitochondrial GSH pool sizes and rates of transport will be studied by tracer-kinetic methods. ..
  13. TUMOR CELL ADHESIVE GLYCOPROTEINS AND METASTASIS
    KENNETH HONN; Fiscal Year: 1993
    ..Recently, we have demonstrated, by Southern blotting, Northern blotting and immunoprecipitation, the presence of alpha- II-b-beta-3 in several human and murine tumor lines...
  14. TUMOR CELL ADHESIVE GLYCOPROTEINS AND METASTASIS
    KENNETH HONN; Fiscal Year: 1992
    ..Recently, we have demonstrated, by Southern blotting, Northern blotting and immunoprecipitation, the presence of alpha- II-b-beta-3 in several human and murine tumor lines...
  15. HORMONAL REGULATION OF INSULIN GENE EXPRESSION
    JAMES REDMON; Fiscal Year: 1992
    ..Such coordinated regulation may be important in glucose homeostasis in physiologic (e.g., exercise, fasting) and pathophysiologic states...
  16. MOLECULAR NEUROTOXICOLOGY OF ORGANOTIN COMPOUNDS
    MELVIN BILLINGSLEY; Fiscal Year: 1990
    ....
  17. UROTHELIAL CELL ACTIVATION IN INTERSTITIAL CYSTITIS
    Monica Liebert; Fiscal Year: 1993
    ..production of cytokines will be evaluated by the messenger RNA (mRNA) polymerase chain reaction (PCR) and by Northern blotting. PCR, will be used because it will allow the evaluation of multiple cytokines from very small samples...
  18. UROTHELIAL CELL ACTIVATION IN INTERSTITIAL CYSTITIS
    Monica Liebert; Fiscal Year: 1991
    ..production of cytokines will be evaluated by the messenger RNA (mRNA) polymerase chain reaction (PCR) and by Northern blotting. PCR, will be used because it will allow the evaluation of multiple cytokines from very small samples...
  19. REGULATION OF HEPATIC P450S BY LOVASTATIN AND OXYSTEROLS
    THOMAS KOCAREK; Fiscal Year: 1993
    ....
  20. STEROID HORMONES AND NEURONAL REGENERATION
    Kathryn Jones; Fiscal Year: 2001
    ..AR binding assays will also be done to determine if E binds to AR in the facial nucleus. ..
  21. STEROID HORMONES AND NEURONAL REGENERATION
    Kathryn Jones; Fiscal Year: 2001
    ..AR binding assays will also be done to determine if E binds to AR in the facial nucleus. ..
  22. STEROID HORMONES AND NEURONAL REGENERATION
    Kathryn Jones; Fiscal Year: 2000
    ..AR binding assays will also be done to determine if E binds to AR in the facial nucleus. ..
  23. STEROID HORMONES AND NEURONAL REGENERATION
    Kathryn Jones; Fiscal Year: 2000
    ..AR binding assays will also be done to determine if E binds to AR in the facial nucleus. ..
  24. STEROID HORMONES AND NEURONAL REGENERATION
    Kathryn Jones; Fiscal Year: 1999
    ..AR binding assays will also be done to determine if E binds to AR in the facial nucleus. ..
  25. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2007
    ....
  26. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2009
    ....
  27. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2006
    ....
  28. Genistein and Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2010
    ....
  29. CONTROL OF GENE EXPRESSION--THE MAJOR CORNEAL PROTEIN
    David Cooper; Fiscal Year: 1992
    ..Both classes Include candidate genes that may potentially utilize corneal specific enhancer/promoter element(s) and corneal specific DNA-binding protein(s)...
  30. ELEVAT SECOND TRIMEST SERUM HOMOCYST (E) INE LEVELS & SUBSEQT RISK OF PREECLAMPSI
    TANYA SORENSEN; Fiscal Year: 2000
    ..Rudolph-Owen LA, Slayden OD, Matrisian LM, Brenner RM. Matrix metalloproteinase expression in Macaca mulatta endometrium Evidence for zone-specific regulatory tissue gradients. Biol Reprod 59:1349-1359, 1998. ..
  31. PLASMINOGEN ACTIVATOR IN INFLAMMATORY LUNG DISEASES
    Robert Sitrin; Fiscal Year: 1992
    ..Hopefully, this information will foster new strategies for further elucidating the role of macrophage-derived PA activity in inflammatory tissue injury and repair...
  32. PLASMINOGEN ACTIVATOR IN INFLAMMATORY LUNG DISEASES
    Robert Sitrin; Fiscal Year: 1991
    ..Hopefully, this information will foster new strategies for further elucidating the role of macrophage-derived PA activity in inflammatory tissue injury and repair...
  33. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2005
    ..The project described in this proposal continues studies of membrane biogenesis and translocation of a major surface-localized protein of S. mutans. ..
  34. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2004
    ..The project described in this proposal continues studies of membrane biogenesis and translocation of a major surface-localized protein of S. mutans. ..
  35. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2007
    ..The project described in this proposal continues studies of membrane biogenesis and translocation of a major surface-localized protein of S. mutans. ..
  36. MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
    L Jeannine Brady; Fiscal Year: 2006
    ..The project described in this proposal continues studies of membrane biogenesis and translocation of a major surface-localized protein of S. mutans. ..
  37. CONTROL OF LYMPHOKINE PRODUCTION:EFFECT OF CYCLOSPORINE
    Angela Granelli Piperno; Fiscal Year: 1990
    ..The induction of lymphokine mRNA has been monitored with an oocyte translation assay and with northern blotting. Primary populations of mouse and human T lymphoblasts, which release large amounts of lymphokine upon ..
  38. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2006
    ....
  39. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2007
    ....
  40. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2010
    ....
  41. B & T LYMPHOCYTE SUBPOPULATIONS IN OCULAR DISEASE
    DANIEL KNOWLES; Fiscal Year: 1991
    ..This will allow us to 5) investigate the etiology and pathogenesis of the inflammatory diseases of the ocular adnexa...
  42. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2005
    ....
  43. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2009
    ....
  44. REGULATION OF GENE EXPRESSION IN PHAGOCYTIC CELLS
    DAVID SKALNIK; Fiscal Year: 1993
    ....
  45. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2006
    ....
  46. REGULATION OF SODIUM CHANNEL EXPRESSION
    ROBERT MAUE; Fiscal Year: 1999
    ....
  47. Cytokines and the blood-brain barrier in the ovine fetus
    Barbara S Stonestreet; Fiscal Year: 2010
    ..The incidence of CP is 40-148/1,000 in premature and 1-2/1,000 in full term infants. This project could provide new insights into novel strategies to prevent brain injury in the human fetus and/or premature infant. ..
  48. Cytokines and the blood-brain barrier in the ovine fetus
    BARBARA STONESTREET; Fiscal Year: 2009
    ..The incidence of CP is 40-148/1,000 in premature and 1-2/1,000 in full term infants. This project could provide new insights into novel strategies to prevent brain injury in the human fetus and/or premature infant. ..
  49. Cytokines and the blood-brain barrier in the ovine fetus
    BARBARA STONESTREET; Fiscal Year: 2009
    ..The incidence of CP is 40-148/1,000 in premature and 1-2/1,000 in full term infants. This project could provide new insights into novel strategies to prevent brain injury in the human fetus and/or premature infant. ..
  50. INBORN ERRORS OF METABOLISM IN CELL CULTURE
    RODY COX; Fiscal Year: 1990
    ..The antibody and cDNA probes will be used to define the molecular basis of FH types I and II. Approaches similar to those described for MSUD will be adapted for the FH studies...
  51. REGULATION OF SODIUM CHANNEL EXPRESSION
    ROBERT MAUE; Fiscal Year: 2000
    ....
  52. Cytokines and the blood-brain barrier in the ovine fetus
    Barbara S Stonestreet; Fiscal Year: 2010
    ..The incidence of CP is 40-148/1,000 in premature and 1-2/1,000 in full term infants. This project could provide new insights into novel strategies to prevent brain injury in the human fetus and/or premature infant. ..
  53. DEVELOPMENT OF INTESTINAL TRANSPORT OF CA++ AND PI
    Fayez Ghishan; Fiscal Year: 1990
    ..These studies will pioneer work on the Na-phosphate transporter and will shed light on the defect in phosphate transport in the (Hyp/y) mouse and eventually in man with hypophosphatemic rickets...