biotinylation

Summary

Summary: Incorporation of biotinyl groups into molecules.

Top Publications

  1. ncbi Proteins exposed at the adult schistosome surface revealed by biotinylation
    Simon Braschi
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    Mol Cell Proteomics 5:347-56. 2006
  2. ncbi Biotinylation of proteins in vivo and in vitro using small peptide tags
    M G Cull
    Avidity, L L C, Eleanor Roosevelt Institute, Denver, Colorado 80206, USA
    Methods Enzymol 326:430-40. 2000
  3. pmc Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice
    Ernie de Boer
    Department of Cell Biology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 100:7480-5. 2003
  4. pmc A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation
    D Beckett
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore 21250, USA
    Protein Sci 8:921-9. 1999
  5. doi Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles
    Lynnette Rios
    Biomolecular Interfaces and Systems Department, Sandia National Laboratories, PO Box 5800, MS 1303, Albuqeurque, NM 87185 1303, USA
    Lab Chip 9:1005-10. 2009
  6. doi Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules
    Till Korten
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307, Dresden, Germany
    Lab Chip 8:1441-7. 2008
  7. ncbi Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis
    Paolo Conrotto
    Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Int J Cancer 123:2856-64. 2008
  8. doi Activity-dependent proteolytic cleavage of neuroligin-1
    Kunimichi Suzuki
    Department of Neuropathology and Neuroscience, The University of Tokyo, Hongo 7 3 1, Bunkyo, Tokyo 113 0033, Japan
    Neuron 76:410-22. 2012
  9. pmc Polycomb purification by in vivo biotinylation tagging reveals cohesin and Trithorax group proteins as interaction partners
    Gero Strübbe
    Department of Biosystems Science and Engineering, Swiss Federal Institute of Technology Zurich, Mattenstrasse 26, 4058 Basel, Switzerland
    Proc Natl Acad Sci U S A 108:5572-7. 2011
  10. ncbi Molecular self-assembly of "nanowires"and "nanospools" using active transport
    Henry Hess
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Nano Lett 5:629-33. 2005

Detail Information

Publications326 found, 100 shown here

  1. ncbi Proteins exposed at the adult schistosome surface revealed by biotinylation
    Simon Braschi
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    Mol Cell Proteomics 5:347-56. 2006
    ..We labeled the most exposed surface proteins using two impermeant biotinylation reagents that differed only in length...
  2. ncbi Biotinylation of proteins in vivo and in vitro using small peptide tags
    M G Cull
    Avidity, L L C, Eleanor Roosevelt Institute, Denver, Colorado 80206, USA
    Methods Enzymol 326:430-40. 2000
  3. pmc Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice
    Ernie de Boer
    Department of Cell Biology, Erasmus Medical Center, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 100:7480-5. 2003
    ..b>Biotinylation is an attractive approach for protein complex purification due to the very high affinity of avidin/..
  4. pmc A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation
    D Beckett
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore 21250, USA
    Protein Sci 8:921-9. 1999
    ..of naturally biotinylated substrates are highly conserved across evolutionary boundaries, and cross-species biotinylation has been demonstrated in several systems...
  5. doi Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles
    Lynnette Rios
    Biomolecular Interfaces and Systems Department, Sandia National Laboratories, PO Box 5800, MS 1303, Albuqeurque, NM 87185 1303, USA
    Lab Chip 9:1005-10. 2009
    ..In this paper we discuss the results of selectivity and motility in single and multiplexed assays...
  6. doi Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules
    Till Korten
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307, Dresden, Germany
    Lab Chip 8:1441-7. 2008
    ....
  7. ncbi Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis
    Paolo Conrotto
    Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Int J Cancer 123:2856-64. 2008
    ....
  8. doi Activity-dependent proteolytic cleavage of neuroligin-1
    Kunimichi Suzuki
    Department of Neuropathology and Neuroscience, The University of Tokyo, Hongo 7 3 1, Bunkyo, Tokyo 113 0033, Japan
    Neuron 76:410-22. 2012
    ..Collectively, neuronal activity-dependent proteolytic processing of NLG1 may negatively regulate the remodeling of spines at excitatory synapses...
  9. pmc Polycomb purification by in vivo biotinylation tagging reveals cohesin and Trithorax group proteins as interaction partners
    Gero Strübbe
    Department of Biosystems Science and Engineering, Swiss Federal Institute of Technology Zurich, Mattenstrasse 26, 4058 Basel, Switzerland
    Proc Natl Acad Sci U S A 108:5572-7. 2011
    ..the dynamic aspects underlying PcG composition and function we have established an inducible version of the biotinylation tagging approach to purify Polycomb and associated factors from Drosophila embryos...
  10. ncbi Molecular self-assembly of "nanowires"and "nanospools" using active transport
    Henry Hess
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Nano Lett 5:629-33. 2005
    ..The integration of molecular motors into the self-assembly process overcomes the trade-off between stability and complexity in thermally activated molecular self-assembly...
  11. ncbi In vivo biotinylation of the vasculature in B-cell lymphoma identifies BST-2 as a target for antibody-based therapy
    Christoph Schliemann
    Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Blood 115:736-44. 2010
    ..Here, we describe the results of a chemical proteomic study, based on the in vivo biotinylation of vascular proteins in lymphoma-bearing mice followed by mass spectrometric and bioinformatic analysis, to ..
  12. pmc Biotinylation is a natural, albeit rare, modification of human histones
    Toshinobu Kuroishi
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Mol Genet Metab 104:537-45. 2011
    ..Recently, a team of investigators failed to detect biotinylated histones and proposed that biotinylation is not a natural modification of histones, but rather an assay artifact...
  13. doi Transport of single cells using an actin bundle-myosin bionanomotor transport system
    Hideyo Takatsuki
    Department of Biological Sciences, Marshall University, Huntington, WV, USA
    Nanotechnology 22:245101. 2011
    ..Cargo unloading from bundles was achieved by incubation with Triton X-100. These data suggest that actin bundling may improve the ability of the myosin motor system for nanotransport applications...
  14. doi Profiling the outer membrane proteome during growth and development of the social bacterium Myxococcus xanthus by selective biotinylation and analyses of outer membrane vesicles
    Jörg Kahnt
    Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Str, Marburg, Germany
    J Proteome Res 9:5197-208. 2010
    ..First, outer membrane proteins were enriched by biotinylation of intact cells using the reagent NHS (N-hydroxysuccinimide)-PEO(12) (polyethylene oxide)-biotin with ..
  15. ncbi Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway
    N Sans
    Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892 8027, USA
    J Neurosci 21:7506-16. 2001
    ..We also show that internalization of GluR1, as triggered by NMDAR activation, does not require SAP97. These results implicate GluR1-SAP97 interactions in mechanisms underlying AMPA receptor targeting...
  16. doi Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells
    Jonghwan Kim
    Division of Hematology Oncology, Department of Pediatric Oncology, Children s Hospital and the Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts 02115, USA
    Nat Protoc 4:506-17. 2009
    ..In dissecting the pluripotent state in mouse embryonic stem (mES) cells, we have used in vivo biotinylation of critical transcription factors for affinity purification of protein complexes and chromatin ..
  17. ncbi Hepatitis C virus NS3 and simian virus 40 T antigen helicases displace streptavidin from 5'-biotinylated oligonucleotides but not from 3'-biotinylated oligonucleotides: evidence for directional bias in translocation on single-stranded DNA
    Patrick D Morris
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Biochemistry 41:2372-8. 2002
    ..The dependence of the reaction with NS3 on the oligonucleotide length suggests that multiple NS3 monomers are necessary for optimal activity...
  18. pmc A monovalent streptavidin with a single femtomolar biotin binding site
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 3:267-73. 2006
    ..Monovalent streptavidin should find general application in biomolecule labeling, single-particle tracking and nanotechnology...
  19. ncbi Exploring minimal biotinylation conditions for biosensor analysis using capture chips
    Giuseppe Papalia
    Center for Biomolecular Interaction Analysis, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Anal Biochem 403:30-5. 2010
    Using Biacore's new regenerateable streptavidin capture (CAP) sensor chips, we investigated a number of biotinylation conditions for target ligands...
  20. pmc Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein
    Gerrit Volkmann
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    PLoS ONE 4:e8381. 2009
    ..However, current methods of site-specific protein labeling are few and with limitations, therefore new methods are needed to satisfy the increasing need and sophistications of protein labeling...
  21. pmc Transsynaptic signaling by activity-dependent cleavage of neuroligin-1
    Rui T Peixoto
    Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 76:396-409. 2012
    ..In turn, NLG1 cleavage depresses synaptic transmission by abruptly reducing presynaptic release probability. Thus, local proteolytic control of synaptic adhesion tunes synaptic transmission during brain development and plasticity...
  22. doi Trafficking of immature DeltaF508-CFTR to the plasma membrane and its detection by biotinylation
    Yishan Luo
    Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada
    Biochem J 419:211-9, 2 p following 219. 2009
    ..in the cytosol of BHK (baby-hamster kidney) cells as a soluble polypeptide it was also labelled after surface biotinylation and pulled down on streptavidin beads...
  23. pmc Modification of the carboxy-terminal flanking region of a universal influenza epitope alters CD4⁺ T-cell repertoire selection
    David K Cole
    Institute of Infection and Immunity, Cardiff University School of Medicine, The Henry Wellcome Building, Cardiff CF14 4XN, Wales, UK
    Nat Commun 3:665. 2012
    ....
  24. doi Efficient and rapid labeling of transplanted cell populations with superparamagnetic iron oxide nanoparticles using cell surface chemical biotinylation for in vivo monitoring by MRI
    Po Wah So
    Preclinical Imaging Unit, Institute of Psychiatry, King s College London, London, UK
    Cell Transplant 19:419-29. 2010
    ..No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro...
  25. ncbi In vivo protein biotinylation and sample preparation for the proteomic identification of organ- and disease-specific antigens accessible from the vasculature
    Christoph Roesli
    Institute of Pharmaceutical Sciences, ETH Zurich, 8093 Zurich, Switzerland
    Nat Protoc 1:192-9. 2006
    ..This technology is applicable to comparative proteomic investigations of differentially expressed, accessible proteins in numerous animal models having different physiological and pathological processes...
  26. ncbi Uptake, localization, and noncarboxylase roles of biotin
    Janos Zempleni
    Department of Nutrition and Health Sciences and Departments of Biochemistry and Animal Science, University of Nebraska at Lincoln, Nebraska 68583 0806, USA
    Annu Rev Nutr 25:175-96. 2005
    ..covalent binding of biotin to histones (DNA-binding proteins), affecting chromatin structure; at least seven biotinylation sites have been identified in human histones...
  27. doi Cell-surface biotinylation to study endocytosis and recycling of occludin
    Noriyuki Nishimura
    Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan
    Methods Mol Biol 440:89-96. 2008
    ..To examine the intracellular transport of transmembrane proteins to and from the cell surface, cell-surface biotinylation is a proven powerful method...
  28. pmc In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia coli biotin ligase and biotin acceptor peptide
    Yoshiaki Maeda
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 76:5785-90. 2010
    ..coli biotin ligase can attach biotin molecules to a lysine residue of biotin acceptor peptide (BAP), and biotinylation of particular BAP-fused proteins in cells was carried out by coexpression of E...
  29. doi Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation
    Cai Huang
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC, USA
    Biotechniques 49:881-6. 2010
    ..Since this method does not require specific antibodies and is inexpensive, sensitive, and reliable, it should be useful for detecting protein-protein interactions in cells...
  30. ncbi Biotin deficiency affects the proliferation of human embryonic palatal mesenchymal cells in culture
    Ryusuke Takechi
    Department of Dietary Environment Analysis, School of Human Science and Environment, Himeji Institute of Technology, University of Hyogo, Himeji, Japan
    J Nutr 138:680-4. 2008
    ..This suppressed proliferation of mesenchymal cells may delay or inhibit the growth of palatal processes in embryos and thus it may partially contribute to the mechanisms for cleft palate induction...
  31. pmc Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation
    Jianlong Wang
    Children s Hospital and the Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts, USA
    Curr Protoc Stem Cell Biol . 2009
    In dissecting the pluripotent state in mouse embryonic stem (ES) cells, we have employed in vivo biotinylation of critical transcription factors for streptavidin affinity purification of protein complexes and constructed a protein-..
  32. pmc Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives
    Andrew Vila
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37232, USA
    Chem Res Toxicol 21:432-44. 2008
    ..Both strategies yielded efficient biotinylation of tagged HNE-protein conjugates, but click chemistry was found to be superior for the recovery of ..
  33. pmc A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli
    P A Smith
    Genetics Institute Inc, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
    Nucleic Acids Res 26:1414-20. 1998
    ..However, in vitro chemical techniques for protein biotinylation are not always successful, with some common problems being a lack of reaction specificity, inactivation of ..
  34. pmc Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking
    Heinrich J G Matthies
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Neurosci 30:7863-77. 2010
    ..Our data demonstrate that AMPH leads to an increase of NET in endosomes of single boutons and varicosities in a Rab11-dependent manner...
  35. pmc In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system
    Andrea Predonzani
    Molecular Immunology Group, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy
    BMC Biotechnol 8:41. 2008
    ..Site-specific biotinylation of proteins in vivo can be achieved by co-expressing in mammalian cells the protein of interest fused to a 15 ..
  36. ncbi Identification of surface proteins of Helicobacter pylori by selective biotinylation, affinity purification, and two-dimensional gel electrophoresis
    Nicolas Sabarth
    Max Planck Institut für Infektionsbiologie, Abteilung Molekulare Biologie, Schumannstrabetae 21 22, D 10117 Berlin, Germany
    J Biol Chem 277:27896-902. 2002
    ..pylori and might be applicable to numerous other human pathogens although larger biotinylation reagents might be required in some cases to prevent permeation of porin channels in the outer membrane.
  37. ncbi A generic tool for biotinylation of tagged proteins in transgenic mice
    Siska Driegen
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    Transgenic Res 14:477-82. 2005
    ..Therefore, this mouse can be crossed to any transgenic mouse to obtain efficient biotinylation of an Avi-tagged protein for the purpose of protein (complex) purification.
  38. ncbi Automated selection of anti-protein aptamers
    J C Cox
    Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    Bioorg Med Chem 9:2525-31. 2001
    ..The increases in throughput brought about by automation should potentiate the application of aptamer technology to the rapidly growing field of proteomics...
  39. pmc An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease
    Hye Young H Kim
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA
    Mol Cell Proteomics 8:2080-9. 2009
    ..Protein catch and photorelease shows that both HSA and ApoA1 in human plasma undergo significant modification by aHNE...
  40. doi Biotinylation reagents for the study of cell surface proteins
    Giuliano Elia
    Mass Spectrometry Resource, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Republic of Ireland
    Proteomics 8:4012-24. 2008
    ..This review will examine the biotin-avidin technology for the study of cell surface proteins, discussing reagents and techniques as well as examples of applications in quantitative proteomics...
  41. ncbi Interaction of tau with the neural membrane cortex is regulated by phosphorylation at sites that are modified in paired helical filaments
    T Maas
    Department of Neurobiology, Interdisziplinäres Zentrum für Neurowissenschaften IZN, University of Heidelberg, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
    J Biol Chem 275:15733-40. 2000
    ....
  42. pmc The role of histone H4 biotinylation in the structure of nucleosomes
    Nina A Filenko
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska, United States of America
    PLoS ONE 6:e16299. 2011
    ..It has been shown that biotinylation of histone H4 at lysine-12 in histone H4 (K12Bio-H4) is associated with repression of a number of genes...
  43. pmc Promiscuous protein biotinylation by Escherichia coli biotin protein ligase
    Eunjoo Choi-Rhee
    Departments of Microbiology and Biochemistry, University of Illinois, Urbana, IL 61801, USA
    Protein Sci 13:3043-50. 2004
    ..The wild type BirA protein is much less active in these reactions. The biotinylation reaction is proximity-dependent in that a greater extent of biotinylation was seen when the mutant ligase was ..
  44. pmc E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells
    Souvik Chakraborty
    Department of Biochemistry and Molecular Biology, Eppley Institute for Research in Cancer and Allied Diseases, Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 285:10761-76. 2010
    ....
  45. ncbi Metabolic biotinylation of lentiviral pseudotypes for scalable paramagnetic microparticle-dependent manipulation
    Darren Nesbeth
    Department of Haematological and Molecular Medicine, Guy s, King s and St Thomas School of Medicine, The Rayne Institute, King s College London, 123 Coldharbour Lane, London SE5 9NU, UK
    Mol Ther 13:814-22. 2006
    ..This new cell line has the potential for widespread application to envelope pseudotypes compatible with lentiviral vector production...
  46. ncbi Trigeminal-reticular connections: possible pathways for nociception-induced cardiovascular reflex responses in the rat
    M J Esser
    Dalhousie University, Faculty of Medicine, Department of Anatomy and Neurobiology, Halifax, Nova Scotia, Canada
    J Comp Neurol 391:526-44. 1998
    ....
  47. pmc Multiple lipid compartments slow vesicle contents release in lipases and serum
    Cecile Boyer
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    ACS Nano 1:176-82. 2007
    ..The vesosome is a better mimic of eukaryotic cell structure and demonstrates the benefits of multiple internal bilayer-enclosed compartments...
  48. ncbi Protein displacement by an assembly of helicase molecules aligned along single-stranded DNA
    Alicia K Byrd
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Nat Struct Mol Biol 11:531-8. 2004
    ..Such a bias leads to protein-protein interactions when the lead monomer stalls owing to the presence of the streptavidin block...
  49. ncbi Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation
    L M Roberts
    XenoPort, Inc, 3410 Central Expressway, Santa Clara, CA 95051, USA
    Neuroscience 155:423-38. 2008
    ..We employed in vivo perfusion of biotinylation reagent through rat brain microvessels to domain-specifically label proteins exposed on the microvessel ..
  50. pmc Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Protoc 3:534-45. 2008
    ..Site-specific biotinylation and streptavidin staining take only a few minutes, while expression of BirA takes 4 d and expression of ..
  51. pmc Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes
    Kyle Cole
    Affymetrix Inc, 3380 Central Expressway, Santa Clara, CA 95051, USA
    Nucleic Acids Res 32:e86. 2004
    ..These results indicate that the detection sensitivity, simplicity (single tube reaction) and speed (2 h) of this direct labeling protocol may be ideal for diagnostic applications that do not require target amplification...
  52. ncbi Biotin supply affects expression of biotin transporters, biotinylation of carboxylases and metabolism of interleukin-2 in Jurkat cells
    Karoline C Manthey
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr 132:887-92. 2002
    Biotin supply may affect transcription of genes and biotinylation of proteins in cells. In this study, Jurkat cells were used to model effects of biotin supply on biotin homeostasis and interleukin-2 metabolism in immune cells...
  53. pmc Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli
    Teegan A Delli-Bovi
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    BMC Biotechnol 10:73. 2010
    ..Isotopic labeling has been a valuable tool for probing the details of the biosynthetic process and assaying the activity of biotin-dependent enzymes, however there is currently no established method for 35S labeling of biotin...
  54. pmc Melting studies of dangling-ended DNA hairpins: effects of end length, loop sequence and biotinylation of loop bases
    Peter V Riccelli
    Department of Chemistry, M C 111, Science and Engineering Building, University of Illinois at Chicago, 845 West Taylor Street, Room 4500, Chicago, IL 60607 7061, USA
    Nucleic Acids Res 30:4088-93. 2002
    ..of 3' single-strand dangling-ends of different lengths, sequence identity of hairpin loop, and hairpin loop biotinylation at different loop residues on DNA hairpin thermodynamic stability were investigated...
  55. pmc Nonenzymatic biotinylation of a biotin carboxyl carrier protein: unusual reactivity of the physiological target lysine
    Emily D Streaker
    Department of Chemistry and Biochemistry and Center for Biological Structure and Organization, University of Maryland, College Park, 20742, USA
    Protein Sci 15:1928-35. 2006
    ..A detailed understanding of the structural basis for the selective biotinylation process has not yet been elucidated...
  56. ncbi Metabolic biotinylation of cell surface receptors for in vivo imaging
    Bakhos A Tannous
    Center for Molecular Imaging Research, Massachusetts General Hospital East, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Methods 3:391-6. 2006
    ..This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo...
  57. pmc Biotinylation, a post-translational modification controlled by the rate of protein-protein association
    Maria Ingaramo
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, Maryland 20742, USA
    J Biol Chem 286:13071-8. 2011
    ..enzyme-substrate pairs the bimolecular rate of association of enzyme with substrate limits post-translational biotinylation. In addition, in noncognate reactions the three enzymes displayed a range of selectivities...
  58. pmc Cytosine methylation in miR-153 gene promoters increases the expression of holocarboxylase synthetase, thereby increasing the abundance of histone H4 biotinylation marks in HEK-293 human kidney cells
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr Biochem 23:635-9. 2012
    Holocarboxylase synthetase (HCS) plays an essential role in catalyzing the biotinylation of carboxylases and histones...
  59. pmc Diversity in functional organization of class I and class II biotin protein ligase
    Sudha Purushothaman
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    PLoS ONE 6:e16850. 2011
    ..This feature is also reflected in the lack of self/promiscuous biotinylation by MtBPL...
  60. ncbi Surface glycoproteomic analysis of hepatocellular carcinoma cells by affinity enrichment and mass spectrometric identification
    Wei Mi
    State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, People s Republic of China
    Glycoconj J 29:411-24. 2012
    ..targeted at membrane glycoproteins involving the affinity capture of membrane glycoproteins using glycan biotinylation labeling on intact cell surfaces...
  61. pmc Compartment-restricted biotinylation reveals novel features of prion protein metabolism in vivo
    Amy B Emerman
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 21:4325-37. 2010
    ..Thus, spatiotemporal tagging has uncovered novel aspects of normal and mutant PrP metabolism and should be readily applicable to the analysis of minor topologic isoforms of other proteins...
  62. pmc A native chromatin purification system for epigenomic profiling in Caenorhabditis elegans
    Siew Loon Ooi
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Nucleic Acids Res 38:e26. 2010
    ..elegans H3.3 gene fused to BioTag, a 23-amino-acid peptide serving as a biotinylation substrate for BirA, in vivo in worms. We found that the fusion BioTag::H3...
  63. ncbi Quantitation of biotin-binding immunoglobulins G, A, and M in Human Sera Using F(ab')2anti-human immunoglobulin-coated microplates
    Makoto Muratsugu
    Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University, Habikino, Osaka 583 8555, Japan
    Biol Pharm Bull 31:507-10. 2008
    ..We quantified B-IgG, B-IgA, and B-IgM in sera from healthy specimens and patients with bronchial asthma, atopic dermatitis, epilepsy, and juvenile rheumatoid arthritis...
  64. pmc High-throughput biotinylation of proteins
    Brian K Kay
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA
    Methods Mol Biol 498:185-96. 2009
    ..We present three complementary approaches for biotinylating proteins in vivo in Escherichia coli or in vitro using chemical or enzymatical reactions all of which can be scaled up to tag large numbers of proteins in parallel...
  65. doi Detection of protein S-nitrosation using irreversible biotinylation procedures (IBP)
    Bo Huang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China
    Free Radic Biol Med 49:447-56. 2010
    ..Then we developed irreversible biotinylation procedures (IBP) to prevent this interference, in which irreversible biotinylation was used to instead of ..
  66. doi A novel, enigmatic histone modification: biotinylation of histones by holocarboxylase synthetase
    Yousef I Hassan
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0806, USA
    Nutr Rev 66:721-5. 2008
    ..Eleven biotinylation sites have been identified in histones H2A, H3, and H4...
  67. doi Protein biotinylation
    Giuliano Elia
    University College Dublin, Dublin, Ireland
    Curr Protoc Protein Sci . 2010
    ..These protocols can be applied not only to labeling in vitro or in tissue culture, but also to in vivo labeling of whole laboratory animals or to ex vivo labeling of surgically resected organs...
  68. doi The avidin-biotin complex (ABC) method and other avidin-biotin binding methods
    Gary L Bratthauer
    Department of Gynecologic and Breast Pathology, Armed Forces Institute of Pathology, Washington, DC, USA
    Methods Mol Biol 588:257-70. 2010
    ..Finally, biotinylated tyramide used in conjunction with peroxidase precipitates even greater amounts of biotin molecules for detection by enzyme-labeled avidin molecules...
  69. ncbi In vivo biotinylation studies: specificity of labelling of reticulated platelets by thiazole orange and mepacrine
    M Robinson
    Department of Haematology, University College Hospital, London, UK
    Br J Haematol 108:859-64. 2000
    Animal in vivo biotinylation studies have demonstrated that thiazole orange (TO) labels the youngest cells in the circulation. TO has since been widely used for the measurement of reticulated platelets...
  70. doi A fully recombinant ELISA using in vivo biotinylated antibody fragments for the detection of potato leafroll virus
    Ahmad Al-Mrabeh
    Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK
    J Virol Methods 159:200-5. 2009
    ..The results demonstrate that scFv reagents derived from synthetic phage display platforms can provide effective alternatives to assays incorporating immune reagents...
  71. pmc Tandem affinity purification of miRNA target mRNAs (TAP-Tar)
    Nora Nonne
    CNRS FRE 2944, Institut Andre Lwoff, Villejuif F 94801 and Université Paris Sud, Villejuif F 94801, France
    Nucleic Acids Res 38:e20. 2010
    ..Our 'TAP-Tar' procedure allows the specific pull down of mRNA targets of miRNA. It is useful for validation of targets predicted in silico, and, potentially, for discovery of previously uncharacterized targets...
  72. pmc Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr Biochem 22:470-5. 2011
    ..We tested the hypothesis that HCS interacts physically with histone H3 for subsequent biotinylation. Co-immunoprecipitation experiments were conducted and provided evidence that HCS co-localizes with histone H3 ..
  73. doi Identification of microRNA target genes in vivo
    Wei Zheng
    Department of Oncology, Shengjing Hospital of China Medical University, Sanhao Street 36, Shenyang 1100021, China
    Mol Biotechnol 47:200-4. 2011
    ..It is useful for validation of targets predicted in silico, and, potentially, for discovery of previously uncharacterized targets...
  74. pmc Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patterns
    Erin M Smith
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 137:2006-12. 2007
    ..e. biotinylation of histones...
  75. ncbi Colorimetric silver detection of DNA microarrays
    I Alexandre
    Laboratoire de Biochimie Cellulaire, Facultes Universitaires Notre Dame de la Paix, 61 rue de Bruxelles, Namur, 5000, Belgium
    Anal Biochem 295:1-8. 2001
    ..The detection limit of both methods was equivalent and corresponds to 1 amol of biotinylated DNA attached on an array. Scanning and data analysis of the array were obtained with a colorimetric-based workstation...
  76. ncbi A novel method for monitoring surface membrane trafficking on hippocampal acute slice preparation
    Judith Thomas-Crusells
    Program for Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Viikki Biocenter, FIN 00014, Helsinki, Finland
    J Neurosci Methods 125:159-66. 2003
    ..Here, we present a method for cell surface protein biotinylation in the acute hippocampal slice, the most commonly used preparation for electrophysiological recordings...
  77. ncbi Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells
    Olga Vagin
    Department of Physiology, School of Medicine at UCLA, Los Angeles, California 90073, USA
    J Biol Chem 280:43159-67. 2005
    ....
  78. ncbi Evaluation of two novel tag-based labelling technologies for site-specific modification of proteins
    Aline Tirat
    Novartis Institutes for BioMedical Research, Discovery Technologies, CH 4056 Basel, Switzerland
    Int J Biol Macromol 39:66-76. 2006
    ..Secondly, we tested in vivo and in vitro site-directed biotinylation with two different tags, consisting of either 15 (AviTag) or 72 amino acids (BioEase tag), which serve as a ..
  79. pmc Repression of transposable elements by histone biotinylation
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr 139:2389-92. 2009
    ..Here, we review recent findings that biotinylation of lysine-12 in histone H4 (H4K12bio) is an epigenetic mechanism to repress long terminal repeat (LTR) ..
  80. ncbi K8 and K12 are biotinylated in human histone H4
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Eur J Biochem 271:2257-63. 2004
    ..g. acetylation, methylation, and biotinylation. We sought to identify amino-acid residues that are biotinylated in histone H4, and to determine whether ..
  81. pmc Imaging activity-dependent regulation of neurexin-neuroligin interactions using trans-synaptic enzymatic biotinylation
    Amar Thyagarajan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Cell 143:456-69. 2010
    ..of intercellular contacts (BLINC), a method for imaging protein interactions based on interaction-dependent biotinylation of a peptide by E...
  82. pmc K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts
    Subhashinee S K Wijeratne
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 21:310-6. 2010
    Covalent modifications of histones play a role in regulating telomere attrition and cellular senescence. Biotinylation of lysine (K) residues in histones, mediated by holocarboxylase synthetase (HCS), is a novel diet-dependent mechanism ..
  83. ncbi Analysis of apolipoprotein E nuclear localization using green fluorescent protein and biotinylation approaches
    Woojin S Kim
    Prince of Wales Medical Research Institute, Randwick, NSW 2031, Australia
    Biochem J 409:701-9. 2008
    ..All three apoE isoforms were equally detected in the nucleus. These studies provide new evidence that apoE may be targeted to the nucleus and shed light on factors that regulate this process...
  84. pmc Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3
    Keyna Kobza
    Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    BMB Rep 41:310-5. 2008
    ..This study tested the hypothesis that BirA ligase catalyzes the biotinylation of eukaryotic histones...
  85. pmc Lysine residues in N-terminal and C-terminal regions of human histone H2A are targets for biotinylation by biotinidase
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 68583 0806, USA
    J Nutr Biochem 17:225-33. 2006
    ..The primary aim of this study was to identify biotinylation sites in histone H2A and its variant H2AX...
  86. pmc K12-biotinylated histone H4 marks heterochromatin in human lymphoblastoma cells
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 18:760-8. 2007
    ..Recently, we reported a novel modification of histones: biotinylation of lysine residues...
  87. pmc Biotinylation of histones represses transposable elements in human and mouse cells and cell lines and in Drosophila melanogaster
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
    J Nutr 138:2316-22. 2008
    ..Collectively, this study reveals a novel diet-dependent epigenetic mechanism that could affect cancer risk...
  88. doi Ubiquitination and endocytosis of cell adhesion molecule DM-GRASP regulate its cell surface presence and affect its role for axon navigation
    Karsten Thelen
    Department of Developmental Neurobiology, University of Heidelberg, D 69120 Heidelberg, Germany
    J Biol Chem 283:32792-801. 2008
    ..Together, our data show that ubiquitination and endocytosis of DM-GRASP in concert regulate its cell surface concentration, which is crucial for its function in axon navigation...
  89. pmc Drosophila melanogaster holocarboxylase synthetase is a chromosomal protein required for normal histone biotinylation, gene transcription patterns, lifespan, and heat tolerance
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 136:2735-42. 2006
    ..Histone biotinylation has been implicated in heterochromatin structures, DNA repair, and mitotic chromosome condensation...
  90. ncbi Assessment of survival of aging erythrocyte in circulation and attendant changes in size and CD147 expression by a novel two step biotinylation method
    Sanjay Khandelwal
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Exp Gerontol 41:855-61. 2006
    ..6mg) resulted in the labeling of erythrocytes released in blood circulation after the first biotinylation step, with a lower intensity of biotin...
  91. ncbi A unique biotin carboxyl carrier protein in archaeon Sulfolobus tokodaii
    Yan Qiu Li
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Kawazu 680 4, Iizuka 820 8502, Japan
    FEBS Lett 580:1536-40. 2006
    ..BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL)...
  92. ncbi Reversible biotinylation phosphoramidite for 5'-end-labeling, phosphorylation, and affinity purification of synthetic oligonucleotides
    Shiyue Fang
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
    Bioconjug Chem 14:80-5. 2003
    A fluoride/amine-cleavable phosphoramidite designed for biotinylation, phosphorylation, and affinity purification of synthetic oligonucleotides was synthesized and coupled efficiently to the 5'-end of DNA on a solid-phase automatic ..
  93. pmc Fluoride-cleavable biotinylation phosphoramidite for 5'-end-labeling and affinity purification of synthetic oligonucleotides
    Shiyue Fang
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
    Nucleic Acids Res 31:708-15. 2003
    A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5'-end of DNA on an automatic synthesizer...
  94. pmc Comparison of the backbone dynamics of the apo- and holo-carboxy-terminal domain of the biotin carboxyl carrier subunit of Escherichia coli acetyl-CoA carboxylase
    X Yao
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 21250, USA
    Protein Sci 8:307-17. 1999
    ..structures provided evidence for small, localized conformational changes in the biotin-binding region upon biotinylation of the protein. These structural changes may be important for regulating specific protein-protein interactions...
  95. ncbi Cutting edge: detection of antigen-specific CD4+ T cells by HLA-DR1 oligomers is dependent on the T cell activation state
    T O Cameron
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 166:741-5. 2001
    ..T cells of identical specificity but in different activation states varied greatly in their ability to be detected by class II MHC oligomers...
  96. ncbi Bacteriophage lambda surface display of a bacterial biotin acceptor domain reveals the minimal peptide size required for biotinylation
    J Stolz
    Friedrich Alexander Universitat Erlangen Nurnberg, Lehrstuhl Botanik II, Erlangen, Germany
    FEBS Lett 440:213-7. 1998
    ..displayed on bacteriophage lambda heads to determine the minimal sequence length that is necessary to effect biotinylation in vivo...
  97. ncbi Covalent modification of an exposed surface turn alters the global conformation of the biotin carrier domain of Escherichia coli acetyl-CoA carboxylase
    A Chapman-Smith
    Department of Biochemistry, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 272:26017-22. 1997
    ..These data indicate that a conformational change accompanies biotinylation of the biotin domain...
  98. ncbi In vivo enzymatic protein biotinylation
    A Chapman-Smith
    Department of Biochemistry, The University of Adelaide, SA, Australia
    Biomol Eng 16:119-25. 1999
    ..b>Biotinylation is a relatively rare modification, with between one and five biotinylated protein species found in different ..
  99. ncbi Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH
    Eliska Svastova
    Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
    FEBS Lett 577:439-45. 2004
    ..Our findings suggest that hypoxia regulates both expression and activity of CA IX in order to enhance the extracellular acidification, which may have important implications for tumor progression...
  100. doi Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate
    Bagautdin Bagautdinov
    RIKEN SPring 8 Center, Harima Institute, Sayo gun, Hyogo, Japan
    J Biol Chem 283:14739-50. 2008
    Biotin protein ligase (BPL) catalyzes the biotinylation of the biotin carboxyl carrier protein (BCCP) only at a special lysine residue. Here we report the first structure of BPL...
  101. ncbi Identification of outer membrane proteins of Yersinia pestis through biotinylation
    Sophie J Smither
    Dstl, Porton Down, Room 201 Bldg 7a, Salisbury, Wilts, SP4 OJQ, UK
    J Microbiol Methods 68:26-31. 2007
    ..Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase...

Research Grants82

  1. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 2001
    ..tropicalis on the FN molecule. In Specific Aim Three, we will use surface biotinylation and immunoprecipitation to characterize other surface proteins in C...
  2. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 2003
    ..tropicalis on the FN molecule. In Specific Aim Three, we will use surface biotinylation and immunoprecipitation to characterize other surface proteins in C...
  3. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 2000
    ..tropicalis on the FN molecule. In Specific Aim Three, we will use surface biotinylation and immunoprecipitation to characterize other surface proteins in C...
  4. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 2002
    ..tropicalis on the FN molecule. In Specific Aim Three, we will use surface biotinylation and immunoprecipitation to characterize other surface proteins in C...
  5. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 1999
    ..tropicalis on the FN molecule. In Specific Aim Three, we will use surface biotinylation and immunoprecipitation to characterize other surface proteins in C...
  6. Cell Biology of Vasopressin-Induced Water Channels
    Dennis Brown; Fiscal Year: 2002
    ..AQP2 membrane delivery and the intracellular pathways followed by recycling AQP2 using immunocytochemistry, biotinylation and membrane fractionation in cultured cells, kidney slices, whole animals and real-time fluorescence ..
  7. Novel Ca receptor signaling pathways for control of renal ion transport
    Richard Miller; Fiscal Year: 2009
    ..Define the mechanism by which the CaR activates WNK1 and WNK4. Cell surface expression (biotinylation) and channel density (whole cell patch) will be used as measures of CaR signaling through the WNK kinases in ..
  8. CELL BIOLOGY OF ION PUMPS SORTING AND FUNCTION
    Michael Caplan; Fiscal Year: 1999
    ..polarized epithelial cells, the fate of chimeric molecules determined by immunoflorescence and surface biotinylation. Individual residues in each subdomain of the TM4 necessary for a proper sorting of each molecule will then be ..
  9. Novel Approaches to Access GPIs and GPI-Anchored Proteins for the Study of GPI An
    Zhongwu Guo; Fiscal Year: 2010
    ..and all nascent proteins destined for GPI anchorage, their reaction with biotinylated GPIs will result in the biotinylation of all GPI-anchored proteins...
  10. Novel Approaches to Access GPIs and GPI-Anchored Proteins for the Study of GPI An
    Zhongwu Guo; Fiscal Year: 2009
    ..and all nascent proteins destined for GPI anchorage, their reaction with biotinylated GPIs will result in the biotinylation of all GPI-anchored proteins...
  11. CELL BIOLOGY OF ION PUMPS SORTING AND FUNCTION
    Michael Caplan; Fiscal Year: 2000
    ..polarized epithelial cells, the fate of chimeric molecules determined by immunoflorescence and surface biotinylation. Individual residues in each subdomain of the TM4 necessary for a proper sorting of each molecule will then be ..
  12. 5-HT transporter function: Interaction of antidepressants and hormones
    Alan Frazer; Fiscal Year: 2010
    ..hormonal effects, experiments are planned to study the plasma membrane distribution of the SERT using biotinylation studies;to examine the role of specific ER subtypes;and to study possible involvement of brain-derived ..
  13. Tumor exosomes as agents of genetic change
    Xandra Breakefield; Fiscal Year: 2009
    ..The advantage of this biotinylation approach is that it allows the imaging of brain tumors with different modalities including Magnetic Resonance (..
  14. EXOCYTIC INSERTION OF POTASSIUM CHANNELS IN MEMBRANES
    William Moran; Fiscal Year: 2001
    ..an electrical resistance method to determine the effect of alanine on enterocyte volume; and 5) cell surface biotinylation and Western blot analysis to determine the relative number of apical membrane K channels...
  15. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2007
    ..B) Surface biotinylation of BK channels in BAEC to determine whether BK channel activation involves the trafficking of BK channels from ..
  16. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2006
    ..B) Surface biotinylation of BK channels in BAEC to determine whether BK channel activation involves the trafficking of BK channels from ..
  17. Red Cell Survival Following Transfusion in Infants
    John Widness; Fiscal Year: 2007
    ..recovery at 24 hr ("PTR24") and long-term mean potential life span ("MPL")) will be determined using RBC biotinylation methodologies developed in our previous PPG...
  18. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2010
    ..B) Surface biotinylation of BK channels in BAEC to determine whether BK channel activation involves the trafficking of BK channels from ..
  19. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2009
    ..B) Surface biotinylation of BK channels in BAEC to determine whether BK channel activation involves the trafficking of BK channels from ..
  20. Role of Myosin VI in CFTR Endocytosis in the Intestine
    Nadia Ameen; Fiscal Year: 2006
    ..We will employ cell biological approaches including surface biotinylation, Western Blotting, immunofluorescece and immunoelectron microscopic localization in all animal and cell ..
  21. REPAIR OF HAIR BUNDLES IN SEA ANEMONES
    GLEN WATSON; Fiscal Year: 1999
    ..Any repair proteins losing biological activity as a result of biotinylation will be used as antigens to generate polyclonal antibodies. The antibodies will be used in immunocytochemistry...
  22. Role of Myosin VI in CFTR Endocytosis in the Intestine
    Nadia Ameen; Fiscal Year: 2007
    ..We will employ cell biological approaches including surface biotinylation, Western Blotting, immunofluorescece and immunoelectron microscopic localization in all animal and cell ..
  23. SODIUM ENTRY INTO AMILORIDE-SENSITIVE EPITHELIA
    Catherine M Fuller; Fiscal Year: 2010
    ..In addition, we will employ surface biotinylation, surface chemiluminescence, co-immunoprecipitation analysis, and macromolecular assembly assays, to verify ..
  24. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2006
    ..Specific affinity biotinylation methods, which proved to be successful in the identification of mRPE65 as a tRE binding protein, will be ..
  25. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2007
    ..Specific affinity biotinylation methods, which proved to be successful in the identification of mRPE65 as a tRE binding protein, will be ..
  26. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2005
    ..Specific affinity biotinylation methods, which proved to be successful in the identification of mRPE65 as a tRE binding protein, will be ..
  27. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2007
    ..abundance on the kinetics of restitution of cellular polarity will be explored using immunohistochemistry and biotinylation studies to track movement of displaced NaK ATPase following injury and during recovery...
  28. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2010
    ..abundance on the kinetics of restitution of cellular polarity will be explored using immunohistochemistry and biotinylation studies to track movement of displaced NaK ATPase following injury and during recovery...
  29. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2006
    ..abundance on the kinetics of restitution of cellular polarity will be explored using immunohistochemistry and biotinylation studies to track movement of displaced NaK ATPase following injury and during recovery...
  30. HUMAN CMV INFECTION OF RETINAL CELLS
    Lenore Pereira; Fiscal Year: 1993
    ..in an affinity system as a protein that binds the receptor in polarized RPE cells labeled by domain-selective biotinylation. The domain on gB that interacts with the receptor will be studied using mutated forms of gB and viral mutants...
  31. Ca2+ Waves in Hepatocytes: Mechanisms and Effects
    Michael H Nathanson; Fiscal Year: 2010
    ..of targeting and insertion of native transporters will be assessed in hepatocytes using immunoblot and biotinylation, and targeting and insertion of fluorescently tagged transporters will be assessed in liver cell lines using ..
  32. Stress, Depression, Serotonin, and Plasticity of Excitatory Transmission
    SCOTT M contact THOMPSON; Fiscal Year: 2010
    ..such as transfection with constitutively active and dominant negative constructs, western blotting, and biotinylation assays, to investigate the actions of 5-HT1B receptors in hippocampal slices taken from control animals and ..
  33. MECHANISM OF ACTION OF VASOPRESSIN
    Dennis Ausiello; Fiscal Year: 1999
    ..V2Rs to monitor these processes by immunocytochemistry, confocal and electron microscopy or cell surface biotinylation in fixed and living cells. Association of the V2R with similarly tagged auxiliary proteins (e.g...
  34. MECHANISM OF ACTION OF VASOPRESSIN
    Dennis Ausiello; Fiscal Year: 2000
    ..V2Rs to monitor these processes by immunocytochemistry, confocal and electron microscopy or cell surface biotinylation in fixed and living cells. Association of the V2R with similarly tagged auxiliary proteins (e.g...
  35. Membrane Proteins of S. mansoni as Vaccine Candidates
    Donald Harn; Fiscal Year: 2002
    ..a glucose transporter protein (SGTP4) that was definitely demonstrated by immunolocalization and surface biotinylation to be exposed at the host/parasite interface in the worm tegument...
  36. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2009
    ..abundance on the kinetics of restitution of cellular polarity will be explored using immunohistochemistry and biotinylation studies to track movement of displaced NaK ATPase following injury and during recovery...
  37. Capture of Ubiquitin Conjugation and Deconjugation Enzyme Substrates
    Robert Cohen; Fiscal Year: 2009
    ..In Aim 1, modified biotin ligase and biotin acceptor peptide constructs will be designed to allow biotinylation only when these two components are brought together by interaction of an enzyme (E3 or DUB) with its substrate...
  38. STRUCTURE AND ORGANIZATION OF THE PHOTOSYSTEM I COMPLEX
    Parag Chitnis; Fiscal Year: 1999
    ..NHS- biotinylation, protease accessibility, and chemical cross-linking studies will be used...
  39. CONFORMATIONAL CHANGES ASSOCIATED WITH K+ CHANNEL GATING
    ROBERT BLAUSTEIN; Fiscal Year: 2000
    ..to introduce single cysteines into the K+ channel sequence, creating potential targets for cysteine-specific biotinylation. Under electrophysiologic recording, oocytes with open channels will be exposed to a biotinylating reagent and ..
  40. MOLECULAR REGULATION AND FUNCTION OF NA/H EXCHANGER-2
    Chung Ming Tse; Fiscal Year: 1999
    ..two dimensional phosphopeptide mapping with separating the cell surface and intracellular forms of NHE2 using biotinylation; (2) to determine whether the increase in V max of NHE2 by growth factors/protein kinases is due to an ..
  41. CONFORMATIONAL CHANGES ASSOCIATED WITH K+ CHANNEL GATING
    ROBERT BLAUSTEIN; Fiscal Year: 2000
    ..to introduce single cysteines into the K+ channel sequence, creating potential targets for cysteine-specific biotinylation. Under electrophysiologic recording, oocytes with open channels will be exposed to a biotinylating reagent and ..
  42. CONFORMATIONAL CHANGES ASSOCIATED WITH K+ CHANNEL GATING
    ROBERT BLAUSTEIN; Fiscal Year: 1999
    ..to introduce single cysteines into the K+ channel sequence, creating potential targets for cysteine-specific biotinylation. Under electrophysiologic recording, oocytes with open channels will be exposed to a biotinylating reagent and ..
  43. TRANSPORT OF AN E COLI VIRULENCE FACTOR--THE K1 CAPSULE
    Richard Silver; Fiscal Year: 2000
    ..face of the cytoplasmic membrane during the translocation process? These studies will include protease and biotinylation accessibility in oriented membrane vesicles...
  44. ACTIVATION OF CFTR MUTANTS IN VIVO FOR CF THERAPY
    MITCHELL DRUMM; Fiscal Year: 1999
    ..and increasing channel number, the quantity of CFTR in the plasma membrane will be measured by cell-surface biotinylation before and after stimulation...
  45. TRANSPORT OF AN E COLI VIRULENCE FACTOR--THE K1 CAPSULE
    Richard Silver; Fiscal Year: 1999
    ..face of the cytoplasmic membrane during the translocation process? These studies will include protease and biotinylation accessibility in oriented membrane vesicles...
  46. Identification of Autism genes that regulate synaptic Nrx/Nlg signaling complexes
    Craig C Garner; Fiscal Year: 2010
    ..Here, we will combine the technologies of proximity labeling via BirA/AP biotinylation (developed by our collaborator Alice Ting at MIT) to label synaptic Nrx/Nlg complexes, bicistronic vectors to ..
  47. INTESTINAL M CELLS: INTERACTION WITH ENTERIC PATHOGENS
    MARIAN NEUTRA; Fiscal Year: 1999
    ..Epithelial isolation, cell surface biotinylation, and Western blot overlay methods will be applied to identify the glycosylated or non-glycosylated M cell ..
  48. INTESTINAL M CELLS: INTERACTION WITH ENTERIC PATHOGENS
    MARIAN NEUTRA; Fiscal Year: 2000
    ..Epithelial isolation, cell surface biotinylation, and Western blot overlay methods will be applied to identify the glycosylated or non-glycosylated M cell ..
  49. CELL SURFACE PROTEINS OF CULTURED ORAL KERATINOCYTES
    Sergio Bustos; Fiscal Year: 1992
    ..Domain-selective biotinylation coupled with precipitation with immobilized lectins will be used to analyze the apical and basal glycoprotein ..
  50. DESENSITIZATION AND DOWN REGULATION OF CALCIUM RECEPTORS
    Dolores Shoback; Fiscal Year: 2002
    ..responses after treating cells with agonists and by quantifying internalization of CaRs using a surface biotinylation assay...
  51. DESENSITIZATION AND DOWN REGULATION OF CALCIUM RECEPTORS
    Dolores Shoback; Fiscal Year: 1999
    ..responses after treating cells with agonists and by quantifying internalization of CaRs using a surface biotinylation assay...
  52. DESENSITIZATION AND DOWN REGULATION OF CALCIUM RECEPTORS
    Dolores Shoback; Fiscal Year: 2000
    ..responses after treating cells with agonists and by quantifying internalization of CaRs using a surface biotinylation assay...
  53. DESENSITIZATION AND DOWN REGULATION OF CALCIUM RECEPTORS
    Dolores Shoback; Fiscal Year: 2001
    ..responses after treating cells with agonists and by quantifying internalization of CaRs using a surface biotinylation assay...
  54. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2002
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  55. STRUCTURE AND FUNCTION OF MEMBRANE TRANSPORT PROTEINS
    Daniel Levy; Fiscal Year: 2000
    ..and functional expression of mEH on the hepatocyte cell surface will be investigated using antibody and biotinylation reagents, confocal fluorescence microscopy, glycosylation site insertion technology, co- immunoprecipitation, ..
  56. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2003
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  57. STRUCTURE AND FUNCTION OF MEMBRANE TRANSPORT PROTEINS
    Daniel Levy; Fiscal Year: 2003
    ..and functional expression of mEH on the hepatocyte cell surface will be investigated using antibody and biotinylation reagents, confocal fluorescence microscopy, glycosylation site insertion technology, co- immunoprecipitation, ..
  58. PHOSPHOINOSITIDES IN NORMAL AND ABNORMAL HUMAN RED CELLS
    George Dale; Fiscal Year: 1991
    ..developed procedure for the isolation of senescent erythrocytes will be utilized; this procedure involves the biotinylation of rabbit erythrocytes...
  59. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2002
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  60. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2004
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  61. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2005
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  62. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2006
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  63. STRUCTURE AND FUNCTION OF MEMBRANE TRANSPORT PROTEINS
    Daniel Levy; Fiscal Year: 2001
    ..and functional expression of mEH on the hepatocyte cell surface will be investigated using antibody and biotinylation reagents, confocal fluorescence microscopy, glycosylation site insertion technology, co- immunoprecipitation, ..
  64. STRUCTURE AND FUNCTION OF MEMBRANE TRANSPORT PROTEINS
    Daniel Levy; Fiscal Year: 2002
    ..and functional expression of mEH on the hepatocyte cell surface will be investigated using antibody and biotinylation reagents, confocal fluorescence microscopy, glycosylation site insertion technology, co- immunoprecipitation, ..
  65. BLOOD/BRAIN BARRIER DRUG DELIVERY OF BDNF IN ISCHEMIA
    William Pardridge; Fiscal Year: 2001
    ..of the OX26 MAb and streptavidin (SA) is prepared in parallel with the carboxyl-directed pegylation and mono-biotinylation of recombinant human BDNF...
  66. BLOOD/BRAIN BARRIER DRUG DELIVERY OF BDNF IN ISCHEMIA
    William Pardridge; Fiscal Year: 2000
    ..of the OX26 MAb and streptavidin (SA) is prepared in parallel with the carboxyl-directed pegylation and mono-biotinylation of recombinant human BDNF...
  67. BLOOD/BRAIN BARRIER DRUG DELIVERY OF BDNF IN ISCHEMIA
    William Pardridge; Fiscal Year: 2002
    ..of the OX26 MAb and streptavidin (SA) is prepared in parallel with the carboxyl-directed pegylation and mono-biotinylation of recombinant human BDNF...
  68. BLOOD/BRAIN BARRIER DRUG DELIVERY OF BDNF IN ISCHEMIA
    William Pardridge; Fiscal Year: 1999
    ..of the OX26 MAb and streptavidin (SA) is prepared in parallel with the carboxyl-directed pegylation and mono-biotinylation of recombinant human BDNF...
  69. BLOOD/BRAIN BARRIER DRUG DELIVERY OF BDNF IN ISCHEMIA
    William Pardridge; Fiscal Year: 2001
    ..of the OX26 MAb and streptavidin (SA) is prepared in parallel with the carboxyl-directed pegylation and mono-biotinylation of recombinant human BDNF...
  70. Junctional exocytosis and breakdown of the intestinal barrier in inflammation
    ANDREI IVANOVICH IVANOV; Fiscal Year: 2012
    ..Vesicle fusion machinery will be analyzed by a combination of biochemical (biotinylation, immunoprecipitation, affinity chromatography), immunocytochemical and genetic (siRNA knock-down of SNARE ..
  71. Junctional exocytosis and breakdown of the intestinal barrier in inflammation
    ANDREI IVANOVICH IVANOV; Fiscal Year: 2010
    ..Vesicle fusion machinery will be analyzed by a combination of biochemical (biotinylation, immunoprecipitation, affinity chromatography), immunocytochemical and genetic (siRNA knock-down of SNARE ..
  72. Biotin sensing and chromatin remodeling by holocarboxylase synthetase
    Janos Zempleni; Fiscal Year: 2009
    ..synthetase (HCS) mediates the binding of biotin to histones (DNA-binding proteins) H2A, H3, and H4, and that biotinylation of histones causes gene repression...
  73. Biotin sensing and chromatin remodeling by holocarboxylase synthetase
    Janos Zempleni; Fiscal Year: 2010
    ..synthetase (HCS) mediates the binding of biotin to histones (DNA-binding proteins) H2A, H3, and H4, and that biotinylation of histones causes gene repression...
  74. Novel histone biotinylation sites and relationships to other epigenetic marks
    JANOS contact ZEMPLENI; Fiscal Year: 2010
    Histones are known to be modified by covalent binding of the vitamin biotin Histone biotinylation is unique among histone modifications in that a metabolic co-factor also functions as a chromatin mark...
  75. Novel histone biotinylation sites and relationships to other epigenetic marks
    Janos Zempleni; Fiscal Year: 2009
    Histones are known to be modified by covalent binding of the vitamin biotin Histone biotinylation is unique among histone modifications in that a metabolic co-factor also functions as a chromatin mark...
  76. Capture of Ubiquitin Conjugation and Deconjugation Enzyme Substrates
    Robert Cohen; Fiscal Year: 2010
    ..In Aim 1, modified biotin ligase and biotin acceptor peptide constructs will be designed to allow biotinylation only when these two components are brought together by interaction of an enzyme (E3 or DUB) with its substrate...
  77. REGULATION OF MEMBRANE EXCITABILITY
    KURT BEAM; Fiscal Year: 2009
    ..that neighbor functionally important sites within the DHPR by exploiting both proteomics and metabolic biotinylation. Accomplishing these specific aims should reveal essential new information about a basic muscle function, EC ..
  78. Melatonin Regulation of MT2 Melatonin Receptors
    MATTHEW GERDIN; Fiscal Year: 2003
    ..negatives of G protein receptor kinases (GRK), arrestins, and dynamin, confocal microscopic and cell surface biotinylation experiments will examine the process of CHO-MT2 receptor internalization...
  79. Dopamine and Glutamate Receptor Interactions
    MARINA WOLF; Fiscal Year: 2006
    ..Aim 2 will further characterize regulation of GluR1 surface expression by glutamate and DA agonists. Surface biotinylation experiments will be used to verify prior results obtained with quantitative immunofluorescence assays...
  80. Melatonin Regulation of MT2 Melatonin Receptors
    MATTHEW GERDIN; Fiscal Year: 2002
    ..negatives of G protein receptor kinases (GRK), arrestins, and dynamin, confocal microscopic and cell surface biotinylation experiments will examine the process of CHO-MT2 receptor internalization...