biotinylation

Summary

Summary: Incorporation of biotinyl groups into molecules.

Top Publications

  1. pmc Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Protoc 3:534-45. 2008
  2. ncbi Proteins exposed at the adult schistosome surface revealed by biotinylation
    Simon Braschi
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    Mol Cell Proteomics 5:347-56. 2006
  3. ncbi Biotinylation of proteins in vivo and in vitro using small peptide tags
    M G Cull
    Avidity, L L C, Eleanor Roosevelt Institute, Denver, Colorado 80206, USA
    Methods Enzymol 326:430-40. 2000
  4. pmc A monovalent streptavidin with a single femtomolar biotin binding site
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 3:267-73. 2006
  5. ncbi Highly sensitive detection of protein toxins by surface plasmon resonance with biotinylation-based inline atom transfer radical polymerization amplification
    Ying Liu
    Department of Chemistry, University of California, Riverside, California 92521, USA
    Anal Chem 82:3679-85. 2010
  6. pmc Modification of the carboxy-terminal flanking region of a universal influenza epitope alters CD4⁺ T-cell repertoire selection
    David K Cole
    Institute of Infection and Immunity, Cardiff University School of Medicine, The Henry Wellcome Building, Cardiff CF14 4XN, Wales, UK
    Nat Commun 3:665. 2012
  7. ncbi Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality
    Lars Iversen
    Bio Nanotechnology Laboratory, Department of Neuroscience and Pharmacology and Nano Science Center, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark
    Langmuir 24:6375-81. 2008
  8. ncbi Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis
    Paolo Conrotto
    Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Int J Cancer 123:2856-64. 2008
  9. ncbi Activity-dependent proteolytic cleavage of neuroligin-1
    Kunimichi Suzuki
    Department of Neuropathology and Neuroscience, The University of Tokyo, Hongo 7 3 1, Bunkyo, Tokyo 113 0033, Japan
    Neuron 76:410-22. 2012
  10. ncbi Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles
    Lynnette Rios
    Biomolecular Interfaces and Systems Department, Sandia National Laboratories, PO Box 5800, MS 1303, Albuqeurque, NM 87185 1303, USA
    Lab Chip 9:1005-10. 2009

Detail Information

Publications346 found, 100 shown here

  1. pmc Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Protoc 3:534-45. 2008
    ..Site-specific biotinylation and streptavidin staining take only a few minutes, while expression of BirA takes 4 d and expression of ..
  2. ncbi Proteins exposed at the adult schistosome surface revealed by biotinylation
    Simon Braschi
    Department of Biology, University of York, York YO10 5DD, United Kingdom
    Mol Cell Proteomics 5:347-56. 2006
    ..We labeled the most exposed surface proteins using two impermeant biotinylation reagents that differed only in length...
  3. ncbi Biotinylation of proteins in vivo and in vitro using small peptide tags
    M G Cull
    Avidity, L L C, Eleanor Roosevelt Institute, Denver, Colorado 80206, USA
    Methods Enzymol 326:430-40. 2000
  4. pmc A monovalent streptavidin with a single femtomolar biotin binding site
    Mark Howarth
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 3:267-73. 2006
    ..Monovalent streptavidin should find general application in biomolecule labeling, single-particle tracking and nanotechnology...
  5. ncbi Highly sensitive detection of protein toxins by surface plasmon resonance with biotinylation-based inline atom transfer radical polymerization amplification
    Ying Liu
    Department of Chemistry, University of California, Riverside, California 92521, USA
    Anal Chem 82:3679-85. 2010
    ....
  6. pmc Modification of the carboxy-terminal flanking region of a universal influenza epitope alters CD4⁺ T-cell repertoire selection
    David K Cole
    Institute of Infection and Immunity, Cardiff University School of Medicine, The Henry Wellcome Building, Cardiff CF14 4XN, Wales, UK
    Nat Commun 3:665. 2012
    ....
  7. ncbi Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality
    Lars Iversen
    Bio Nanotechnology Laboratory, Department of Neuroscience and Pharmacology and Nano Science Center, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark
    Langmuir 24:6375-81. 2008
    ..While three strategies resulted in similar patterning efficiencies, one particular approach--namely templated assembly of SFH directly on streptavidin-BG patterns--resulted in an order of magnitude increase in patterning efficiency...
  8. ncbi Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis
    Paolo Conrotto
    Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Int J Cancer 123:2856-64. 2008
    ....
  9. ncbi Activity-dependent proteolytic cleavage of neuroligin-1
    Kunimichi Suzuki
    Department of Neuropathology and Neuroscience, The University of Tokyo, Hongo 7 3 1, Bunkyo, Tokyo 113 0033, Japan
    Neuron 76:410-22. 2012
    ..Collectively, neuronal activity-dependent proteolytic processing of NLG1 may negatively regulate the remodeling of spines at excitatory synapses...
  10. ncbi Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles
    Lynnette Rios
    Biomolecular Interfaces and Systems Department, Sandia National Laboratories, PO Box 5800, MS 1303, Albuqeurque, NM 87185 1303, USA
    Lab Chip 9:1005-10. 2009
    ..In this paper we discuss the results of selectivity and motility in single and multiplexed assays...
  11. pmc Biotinylation is a natural, albeit rare, modification of human histones
    Toshinobu Kuroishi
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Mol Genet Metab 104:537-45. 2011
    ..Recently, a team of investigators failed to detect biotinylated histones and proposed that biotinylation is not a natural modification of histones, but rather an assay artifact...
  12. ncbi Profiling the outer membrane proteome during growth and development of the social bacterium Myxococcus xanthus by selective biotinylation and analyses of outer membrane vesicles
    Jörg Kahnt
    Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Str, Marburg, Germany
    J Proteome Res 9:5197-208. 2010
    ..First, outer membrane proteins were enriched by biotinylation of intact cells using the reagent NHS (N-hydroxysuccinimide)-PEO(12) (polyethylene oxide)-biotin with ..
  13. ncbi Two discontinuous segments in the carboxyl terminus are required for membrane targeting of the rat gamma-aminobutyric acid transporter-1 (GAT1)
    Hesso Farhan
    Institute of Pharmacology, Medical University of Vienna, Währinger Strasse 13a, A 1090 Vienna, Austria
    J Biol Chem 279:28553-63. 2004
    ....
  14. pmc Polycomb purification by in vivo biotinylation tagging reveals cohesin and Trithorax group proteins as interaction partners
    Gero Strübbe
    Department of Biosystems Science and Engineering, Swiss Federal Institute of Technology Zurich, Mattenstrasse 26, 4058 Basel, Switzerland
    Proc Natl Acad Sci U S A 108:5572-7. 2011
    ..the dynamic aspects underlying PcG composition and function we have established an inducible version of the biotinylation tagging approach to purify Polycomb and associated factors from Drosophila embryos...
  15. ncbi Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells
    Jonghwan Kim
    Division of Hematology Oncology, Department of Pediatric Oncology, Children s Hospital and the Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts 02115, USA
    Nat Protoc 4:506-17. 2009
    ..In dissecting the pluripotent state in mouse embryonic stem (mES) cells, we have used in vivo biotinylation of critical transcription factors for affinity purification of protein complexes and chromatin ..
  16. ncbi In vivo protein biotinylation for identification of organ-specific antigens accessible from the vasculature
    Jascha N Rybak
    Swiss Federal Institute of Technology, Zurich, Switzerland
    Nat Methods 2:291-8. 2005
    ..In the present study, in vivo biotinylation procedure led to the identification of hundreds of proteins in different mouse organs, including some showing ..
  17. ncbi Interaction of tau with the neural membrane cortex is regulated by phosphorylation at sites that are modified in paired helical filaments
    T Maas
    Department of Neurobiology, Interdisziplinäres Zentrum für Neurowissenschaften IZN, University of Heidelberg, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
    J Biol Chem 275:15733-40. 2000
    ....
  18. ncbi Automated selection of anti-protein aptamers
    J C Cox
    Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    Bioorg Med Chem 9:2525-31. 2001
    ..The increases in throughput brought about by automation should potentiate the application of aptamer technology to the rapidly growing field of proteomics...
  19. ncbi Hepatitis C virus NS3 and simian virus 40 T antigen helicases displace streptavidin from 5'-biotinylated oligonucleotides but not from 3'-biotinylated oligonucleotides: evidence for directional bias in translocation on single-stranded DNA
    Patrick D Morris
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Biochemistry 41:2372-8. 2002
    ..The dependence of the reaction with NS3 on the oligonucleotide length suggests that multiple NS3 monomers are necessary for optimal activity...
  20. ncbi Metabolic biotinylation of cell surface receptors for in vivo imaging
    Bakhos A Tannous
    Center for Molecular Imaging Research, Massachusetts General Hospital East, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Methods 3:391-6. 2006
    ..This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo...
  21. pmc A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation
    D Beckett
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore 21250, USA
    Protein Sci 8:921-9. 1999
    ..of naturally biotinylated substrates are highly conserved across evolutionary boundaries, and cross-species biotinylation has been demonstrated in several systems...
  22. pmc Real-time biomolecular binding detection using a sensitive photonic crystal biosensor
    Yunbo Guo
    Center for Ultrafast Optical Science, Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, USA
    Anal Chem 82:5211-8. 2010
    ..By all important metrics for sensitivity, we anticipate this photonic crystal biosensor will provide new capabilities for highly sensitive measurements of biomolecular binding...
  23. pmc Novel bilobe components in Trypanosoma brucei identified using proximity-dependent biotinylation
    Brooke Morriswood
    Max F Perutz Laboratories, University of Vienna and Medical University of Vienna, Vienna, Austria
    Eukaryot Cell 12:356-67. 2013
    ..This remarkable success validates BioID as a new tool for the study of unicellular eukaryotes in particular and the eukaryotic cytoskeleton in general...
  24. ncbi Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin
    Rajgopal Govindarajan
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA
    J Biol Chem 277:50087-97. 2002
    ..Our results suggest that impaired trafficking, and not the inability to form gap junctions, is the major cause of communication deficiency in human prostate cancer cell lines...
  25. ncbi Transport of single cells using an actin bundle-myosin bionanomotor transport system
    Hideyo Takatsuki
    Department of Biological Sciences, Marshall University, Huntington, WV, USA
    Nanotechnology 22:245101. 2011
    ..Cargo unloading from bundles was achieved by incubation with Triton X-100. These data suggest that actin bundling may improve the ability of the myosin motor system for nanotransport applications...
  26. pmc Transsynaptic signaling by activity-dependent cleavage of neuroligin-1
    Rui T Peixoto
    Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 76:396-409. 2012
    ..In turn, NLG1 cleavage depresses synaptic transmission by abruptly reducing presynaptic release probability. Thus, local proteolytic control of synaptic adhesion tunes synaptic transmission during brain development and plasticity...
  27. pmc In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia coli biotin ligase and biotin acceptor peptide
    Yoshiaki Maeda
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 76:5785-90. 2010
    ..coli biotin ligase can attach biotin molecules to a lysine residue of biotin acceptor peptide (BAP), and biotinylation of particular BAP-fused proteins in cells was carried out by coexpression of E...
  28. ncbi Uptake, localization, and noncarboxylase roles of biotin
    Janos Zempleni
    Department of Nutrition and Health Sciences and Departments of Biochemistry and Animal Science, University of Nebraska at Lincoln, Nebraska 68583 0806, USA
    Annu Rev Nutr 25:175-96. 2005
    ..covalent binding of biotin to histones (DNA-binding proteins), affecting chromatin structure; at least seven biotinylation sites have been identified in human histones...
  29. ncbi Exploring minimal biotinylation conditions for biosensor analysis using capture chips
    Giuseppe Papalia
    Center for Biomolecular Interaction Analysis, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Anal Biochem 403:30-5. 2010
    Using Biacore's new regenerateable streptavidin capture (CAP) sensor chips, we investigated a number of biotinylation conditions for target ligands...
  30. ncbi In vivo protein biotinylation and sample preparation for the proteomic identification of organ- and disease-specific antigens accessible from the vasculature
    Christoph Roesli
    Institute of Pharmaceutical Sciences, ETH Zurich, 8093 Zurich, Switzerland
    Nat Protoc 1:192-9. 2006
    ..This technology is applicable to comparative proteomic investigations of differentially expressed, accessible proteins in numerous animal models having different physiological and pathological processes...
  31. ncbi Biotin deficiency affects the proliferation of human embryonic palatal mesenchymal cells in culture
    Ryusuke Takechi
    Department of Dietary Environment Analysis, School of Human Science and Environment, Himeji Institute of Technology, University of Hyogo, Himeji, Japan
    J Nutr 138:680-4. 2008
    ..This suppressed proliferation of mesenchymal cells may delay or inhibit the growth of palatal processes in embryos and thus it may partially contribute to the mechanisms for cleft palate induction...
  32. ncbi Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation
    Cai Huang
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC, USA
    Biotechniques 49:881-6. 2010
    ..Since this method does not require specific antibodies and is inexpensive, sensitive, and reliable, it should be useful for detecting protein-protein interactions in cells...
  33. pmc Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation
    Jianlong Wang
    Children s Hospital and the Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts, USA
    Curr Protoc Stem Cell Biol . 2009
    In dissecting the pluripotent state in mouse embryonic stem (ES) cells, we have employed in vivo biotinylation of critical transcription factors for streptavidin affinity purification of protein complexes and constructed a protein-..
  34. pmc A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli
    P A Smith
    Genetics Institute Inc, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
    Nucleic Acids Res 26:1414-20. 1998
    ..However, in vitro chemical techniques for protein biotinylation are not always successful, with some common problems being a lack of reaction specificity, inactivation of ..
  35. pmc Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking
    Heinrich J G Matthies
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Neurosci 30:7863-77. 2010
    ..Our data demonstrate that AMPH leads to an increase of NET in endosomes of single boutons and varicosities in a Rab11-dependent manner...
  36. pmc In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system
    Andrea Predonzani
    Molecular Immunology Group, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy
    BMC Biotechnol 8:41. 2008
    ..Site-specific biotinylation of proteins in vivo can be achieved by co-expressing in mammalian cells the protein of interest fused to a 15 ..
  37. pmc Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing
    Andreas Gnirke
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Biotechnol 27:182-9. 2009
    ..One lane of Illumina sequence was sufficient to call high-confidence genotypes for 89% of the targeted exon space...
  38. pmc Use of yeast-secreted in vivo biotinylated recombinant antibodies (Biobodies) in bead-based ELISA
    Nathalie Scholler
    School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 96104 6069, USA
    Clin Cancer Res 14:2647-55. 2008
    ..Mouse monoclonal antibodies (mAb) and polyclonal antibodies (pAb) are commonly used, but because their production is lengthy and costly, recombinant antibodies are emerging as an attractive alternative...
  39. ncbi Biotinylation reagents for the study of cell surface proteins
    Giuliano Elia
    Mass Spectrometry Resource, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Republic of Ireland
    Proteomics 8:4012-24. 2008
    ..This review will examine the biotin-avidin technology for the study of cell surface proteins, discussing reagents and techniques as well as examples of applications in quantitative proteomics...
  40. ncbi A generic tool for biotinylation of tagged proteins in transgenic mice
    Siska Driegen
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    Transgenic Res 14:477-82. 2005
    ..Therefore, this mouse can be crossed to any transgenic mouse to obtain efficient biotinylation of an Avi-tagged protein for the purpose of protein (complex) purification.
  41. pmc An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease
    Hye Young H Kim
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA
    Mol Cell Proteomics 8:2080-9. 2009
    ..Protein catch and photorelease shows that both HSA and ApoA1 in human plasma undergo significant modification by aHNE...
  42. ncbi Cell-surface biotinylation to study endocytosis and recycling of occludin
    Noriyuki Nishimura
    Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan
    Methods Mol Biol 440:89-96. 2008
    ..To examine the intracellular transport of transmembrane proteins to and from the cell surface, cell-surface biotinylation is a proven powerful method...
  43. ncbi Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules
    Till Korten
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307, Dresden, Germany
    Lab Chip 8:1441-7. 2008
    ....
  44. ncbi Identification of surface proteins of Helicobacter pylori by selective biotinylation, affinity purification, and two-dimensional gel electrophoresis
    Nicolas Sabarth
    Max Planck Institut für Infektionsbiologie, Abteilung Molekulare Biologie, Schumannstrabetae 21 22, D 10117 Berlin, Germany
    J Biol Chem 277:27896-902. 2002
    ..pylori and might be applicable to numerous other human pathogens although larger biotinylation reagents might be required in some cases to prevent permeation of porin channels in the outer membrane.
  45. ncbi Molecular self-assembly of "nanowires"and "nanospools" using active transport
    Henry Hess
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Nano Lett 5:629-33. 2005
    ..The integration of molecular motors into the self-assembly process overcomes the trade-off between stability and complexity in thermally activated molecular self-assembly...
  46. ncbi Assessment of survival of aging erythrocyte in circulation and attendant changes in size and CD147 expression by a novel two step biotinylation method
    Sanjay Khandelwal
    School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
    Exp Gerontol 41:855-61. 2006
    ..6mg) resulted in the labeling of erythrocytes released in blood circulation after the first biotinylation step, with a lower intensity of biotin...
  47. ncbi In vivo biotinylation of the vasculature in B-cell lymphoma identifies BST-2 as a target for antibody-based therapy
    Christoph Schliemann
    Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Blood 115:736-44. 2010
    ..Here, we describe the results of a chemical proteomic study, based on the in vivo biotinylation of vascular proteins in lymphoma-bearing mice followed by mass spectrometric and bioinformatic analysis, to ..
  48. ncbi Cutting edge: detection of antigen-specific CD4+ T cells by HLA-DR1 oligomers is dependent on the T cell activation state
    T O Cameron
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 166:741-5. 2001
    ..T cells of identical specificity but in different activation states varied greatly in their ability to be detected by class II MHC oligomers...
  49. ncbi Metabolic biotinylation of lentiviral pseudotypes for scalable paramagnetic microparticle-dependent manipulation
    Darren Nesbeth
    Department of Haematological and Molecular Medicine, Guy s, King s and St Thomas School of Medicine, The Rayne Institute, King s College London, 123 Coldharbour Lane, London SE5 9NU, UK
    Mol Ther 13:814-22. 2006
    ..This new cell line has the potential for widespread application to envelope pseudotypes compatible with lentiviral vector production...
  50. pmc The role of histone H4 biotinylation in the structure of nucleosomes
    Nina A Filenko
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska, United States of America
    PLoS ONE 6:e16299. 2011
    ..It has been shown that biotinylation of histone H4 at lysine-12 in histone H4 (K12Bio-H4) is associated with repression of a number of genes...
  51. pmc Promiscuous protein biotinylation by Escherichia coli biotin protein ligase
    Eunjoo Choi-Rhee
    Departments of Microbiology and Biochemistry, University of Illinois, Urbana, IL 61801, USA
    Protein Sci 13:3043-50. 2004
    ..The wild type BirA protein is much less active in these reactions. The biotinylation reaction is proximity-dependent in that a greater extent of biotinylation was seen when the mutant ligase was ..
  52. ncbi Trigeminal-reticular connections: possible pathways for nociception-induced cardiovascular reflex responses in the rat
    M J Esser
    Dalhousie University, Faculty of Medicine, Department of Anatomy and Neurobiology, Halifax, Nova Scotia, Canada
    J Comp Neurol 391:526-44. 1998
    ....
  53. ncbi Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation
    L M Roberts
    XenoPort, Inc, 3410 Central Expressway, Santa Clara, CA 95051, USA
    Neuroscience 155:423-38. 2008
    ..We employed in vivo perfusion of biotinylation reagent through rat brain microvessels to domain-specifically label proteins exposed on the microvessel ..
  54. pmc Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes
    Kyle Cole
    Affymetrix Inc, 3380 Central Expressway, Santa Clara, CA 95051, USA
    Nucleic Acids Res 32:e86. 2004
    ..These results indicate that the detection sensitivity, simplicity (single tube reaction) and speed (2 h) of this direct labeling protocol may be ideal for diagnostic applications that do not require target amplification...
  55. ncbi Trafficking of immature DeltaF508-CFTR to the plasma membrane and its detection by biotinylation
    Yishan Luo
    Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada
    Biochem J 419:211-9, 2 p following 219. 2009
    ..in the cytosol of BHK (baby-hamster kidney) cells as a soluble polypeptide it was also labelled after surface biotinylation and pulled down on streptavidin beads...
  56. pmc Site directed biotinylation of filamentous phage structural proteins
    Larisa Smelyanski
    Department of Cell Research and Immunology, George S, Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978 Israel
    Virol J 8:495. 2011
    ..The in vivo and in vitro biotinylation of the various phages were compared...
  57. ncbi Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function
    Bong Kyung Shin
    Departments of Pediatrics and Pathology, University of Michigan, Ann Arbor, Michigan 48109 0656, USA
    J Biol Chem 278:7607-16. 2003
    ..Surface membrane proteins of viable, intact cells were subjected to biotinylation then affinity-captured and purified on monomeric avidin columns...
  58. pmc Multiple lipid compartments slow vesicle contents release in lipases and serum
    Cecile Boyer
    Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
    ACS Nano 1:176-82. 2007
    ..The vesosome is a better mimic of eukaryotic cell structure and demonstrates the benefits of multiple internal bilayer-enclosed compartments...
  59. ncbi Role of N- or C-terminal biotinylation in autoantibody recognition of citrullin containing filaggrin epitope peptides in rheumatoid arthritis
    Fruzsina Babos
    Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eotvos Lorand University, Pazmany Peter setany 1 A, H 1117 Budapest, Hungary
    Bioconjug Chem 24:817-27. 2013
    ..However, the presence and position of the biotin moiety has a pronounced effect on the conformation of the 5-mer epitope core peptides, while it does not alter the secondary structure of the 19-mer epitope region peptides...
  60. pmc Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein
    Gerrit Volkmann
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    PLoS ONE 4:e8381. 2009
    ..However, current methods of site-specific protein labeling are few and with limitations, therefore new methods are needed to satisfy the increasing need and sophistications of protein labeling...
  61. ncbi The replicative helicases of bacteria, archaea, and eukarya can unwind RNA-DNA hybrid substrates
    Jae Ho Shin
    University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology, Rockville, Maryland 20850, USA
    J Biol Chem 281:26914-21. 2006
    ..Thus, the results presented here may suggest a new role for the replicative helicases during chromosomal replication or in other cellular processes...
  62. pmc Efficient detection of proteins retro-translocated from the ER to the cytosol by in vivo biotinylation
    Gianluca Petris
    International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
    PLoS ONE 6:e23712. 2011
    ..We observed specific mono-biotinylation of cytosolically dislocated molecules, resulting in a novel, reliable way of determining the extent of retro-..
  63. ncbi A novel reactive ester derivative of biotin with reduced membrane permeability for in vivo biotinylation experiments
    Verena Strassberger
    Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland
    Proteomics 10:3544-8. 2010
    ..A novel, triply charged biotinylation reagent, NHS-β-Ala-(L-Asp)(3)-biotin, was synthesized and validated in terms of its applicability for in vivo ..
  64. ncbi Biotinylation of silicon-doped hydroxyapatite: a new approach to protein fixation for bone tissue regeneration
    Alejandro Baeza
    Departamento de Quimica Inorganica y Bioinorganica, Facultad de Farmacia, Universidad Complutense de Madrid, E 28040 Madrid, Spain
    Acta Biomater 6:743-9. 2010
    ..Bioceramic biotinylation has been performed by esterification reaction between the OH groups of hydroxyapatite and COOH groups of ..
  65. pmc Holocarboxylase synthetase interacts physically with euchromatic histone-lysine N-methyltransferase, linking histone biotinylation with methylation events
    Yong Li
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 24:1446-52. 2013
    ..HCS) catalyzes the binding of the vitamin biotin to histones H3 and H4, thereby creating rare histone biotinylation marks in the epigenome...
  66. ncbi Protein complexes and target genes identification by in vivo biotinylation: the STAT5 paradigm
    Eleni Katsantoni
    Hematology Oncology Division, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece
    Sci Signal 5:pt13. 2012
    ..Presentation provides an overview on STATs, focusing on STAT5, and describes an in vivo biotinylation methodology applied in mammalian cells for identification of STAT5-containing protein complexes and ..
  67. pmc E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells
    Souvik Chakraborty
    Department of Biochemistry and Molecular Biology, Eppley Institute for Research in Cancer and Allied Diseases, Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 285:10761-76. 2010
    ....
  68. pmc Diversity in functional organization of class I and class II biotin protein ligase
    Sudha Purushothaman
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    PLoS ONE 6:e16850. 2011
    ..This feature is also reflected in the lack of self/promiscuous biotinylation by MtBPL...
  69. ncbi A novel, enigmatic histone modification: biotinylation of histones by holocarboxylase synthetase
    Yousef I Hassan
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0806, USA
    Nutr Rev 66:721-5. 2008
    ..Eleven biotinylation sites have been identified in histones H2A, H3, and H4...
  70. pmc High-throughput biotinylation of proteins
    Brian K Kay
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA
    Methods Mol Biol 498:185-96. 2009
    ..We present three complementary approaches for biotinylating proteins in vivo in Escherichia coli or in vitro using chemical or enzymatical reactions all of which can be scaled up to tag large numbers of proteins in parallel...
  71. pmc Biotinylation, a post-translational modification controlled by the rate of protein-protein association
    Maria Ingaramo
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, Maryland 20742, USA
    J Biol Chem 286:13071-8. 2011
    ..enzyme-substrate pairs the bimolecular rate of association of enzyme with substrate limits post-translational biotinylation. In addition, in noncognate reactions the three enzymes displayed a range of selectivities...
  72. ncbi Quantitation of biotin-binding immunoglobulins G, A, and M in Human Sera Using F(ab')2anti-human immunoglobulin-coated microplates
    Makoto Muratsugu
    Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University, Habikino, Osaka 583 8555, Japan
    Biol Pharm Bull 31:507-10. 2008
    ..We quantified B-IgG, B-IgA, and B-IgM in sera from healthy specimens and patients with bronchial asthma, atopic dermatitis, epilepsy, and juvenile rheumatoid arthritis...
  73. ncbi A fully recombinant ELISA using in vivo biotinylated antibody fragments for the detection of potato leafroll virus
    Ahmad Al-Mrabeh
    Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK
    J Virol Methods 159:200-5. 2009
    ..The results demonstrate that scFv reagents derived from synthetic phage display platforms can provide effective alternatives to assays incorporating immune reagents...
  74. pmc Cytosine methylation in miR-153 gene promoters increases the expression of holocarboxylase synthetase, thereby increasing the abundance of histone H4 biotinylation marks in HEK-293 human kidney cells
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr Biochem 23:635-9. 2012
    Holocarboxylase synthetase (HCS) plays an essential role in catalyzing the biotinylation of carboxylases and histones...
  75. pmc Tandem affinity purification of miRNA target mRNAs (TAP-Tar)
    Nora Nonne
    CNRS FRE 2944, Institut Andre Lwoff, Villejuif F 94801 and Université Paris Sud, Villejuif F 94801, France
    Nucleic Acids Res 38:e20. 2010
    ..Our 'TAP-Tar' procedure allows the specific pull down of mRNA targets of miRNA. It is useful for validation of targets predicted in silico, and, potentially, for discovery of previously uncharacterized targets...
  76. ncbi Identification of microRNA target genes in vivo
    Wei Zheng
    Department of Oncology, Shengjing Hospital of China Medical University, Sanhao Street 36, Shenyang 1100021, China
    Mol Biotechnol 47:200-4. 2011
    ..It is useful for validation of targets predicted in silico, and, potentially, for discovery of previously uncharacterized targets...
  77. ncbi Protein biotinylation
    Giuliano Elia
    University College Dublin, Dublin, Ireland
    Curr Protoc Protein Sci . 2010
    ..These protocols can be applied not only to labeling in vitro or in tissue culture, but also to in vivo labeling of whole laboratory animals or to ex vivo labeling of surgically resected organs...
  78. pmc Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr Biochem 22:470-5. 2011
    ..We tested the hypothesis that HCS interacts physically with histone H3 for subsequent biotinylation. Co-immunoprecipitation experiments were conducted and provided evidence that HCS co-localizes with histone H3 ..
  79. ncbi Detection of protein S-nitrosation using irreversible biotinylation procedures (IBP)
    Bo Huang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China
    Free Radic Biol Med 49:447-56. 2010
    ..Then we developed irreversible biotinylation procedures (IBP) to prevent this interference, in which irreversible biotinylation was used to instead of ..
  80. ncbi The avidin-biotin complex (ABC) method and other avidin-biotin binding methods
    Gary L Bratthauer
    Department of Gynecologic and Breast Pathology, Armed Forces Institute of Pathology, Washington, DC, USA
    Methods Mol Biol 588:257-70. 2010
    ..Finally, biotinylated tyramide used in conjunction with peroxidase precipitates even greater amounts of biotin molecules for detection by enzyme-labeled avidin molecules...
  81. pmc A native chromatin purification system for epigenomic profiling in Caenorhabditis elegans
    Siew Loon Ooi
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Nucleic Acids Res 38:e26. 2010
    ..elegans H3.3 gene fused to BioTag, a 23-amino-acid peptide serving as a biotinylation substrate for BirA, in vivo in worms. We found that the fusion BioTag::H3...
  82. pmc Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli
    Teegan A Delli-Bovi
    Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    BMC Biotechnol 10:73. 2010
    ..Isotopic labeling has been a valuable tool for probing the details of the biosynthetic process and assaying the activity of biotin-dependent enzymes, however there is currently no established method for 35S labeling of biotin...
  83. pmc Compartment-restricted biotinylation reveals novel features of prion protein metabolism in vivo
    Amy B Emerman
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 21:4325-37. 2010
    ..Thus, spatiotemporal tagging has uncovered novel aspects of normal and mutant PrP metabolism and should be readily applicable to the analysis of minor topologic isoforms of other proteins...
  84. ncbi Biotin supply affects expression of biotin transporters, biotinylation of carboxylases and metabolism of interleukin-2 in Jurkat cells
    Karoline C Manthey
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr 132:887-92. 2002
    Biotin supply may affect transcription of genes and biotinylation of proteins in cells. In this study, Jurkat cells were used to model effects of biotin supply on biotin homeostasis and interleukin-2 metabolism in immune cells...
  85. pmc Nonenzymatic biotinylation of a biotin carboxyl carrier protein: unusual reactivity of the physiological target lysine
    Emily D Streaker
    Department of Chemistry and Biochemistry and Center for Biological Structure and Organization, University of Maryland, College Park, 20742, USA
    Protein Sci 15:1928-35. 2006
    ..A detailed understanding of the structural basis for the selective biotinylation process has not yet been elucidated...
  86. ncbi In vivo biotinylation studies: specificity of labelling of reticulated platelets by thiazole orange and mepacrine
    M Robinson
    Department of Haematology, University College Hospital, London, UK
    Br J Haematol 108:859-64. 2000
    Animal in vivo biotinylation studies have demonstrated that thiazole orange (TO) labels the youngest cells in the circulation. TO has since been widely used for the measurement of reticulated platelets...
  87. ncbi Surface glycoproteomic analysis of hepatocellular carcinoma cells by affinity enrichment and mass spectrometric identification
    Wei Mi
    State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, People s Republic of China
    Glycoconj J 29:411-24. 2012
    ..targeted at membrane glycoproteins involving the affinity capture of membrane glycoproteins using glycan biotinylation labeling on intact cell surfaces...
  88. pmc Melting studies of dangling-ended DNA hairpins: effects of end length, loop sequence and biotinylation of loop bases
    Peter V Riccelli
    Department of Chemistry, M C 111, Science and Engineering Building, University of Illinois at Chicago, 845 West Taylor Street, Room 4500, Chicago, IL 60607 7061, USA
    Nucleic Acids Res 30:4088-93. 2002
    ..of 3' single-strand dangling-ends of different lengths, sequence identity of hairpin loop, and hairpin loop biotinylation at different loop residues on DNA hairpin thermodynamic stability were investigated...
  89. ncbi Colorimetric silver detection of DNA microarrays
    I Alexandre
    Laboratoire de Biochimie Cellulaire, Facultes Universitaires Notre Dame de la Paix, 61 rue de Bruxelles, Namur, 5000, Belgium
    Anal Biochem 295:1-8. 2001
    ..The detection limit of both methods was equivalent and corresponds to 1 amol of biotinylated DNA attached on an array. Scanning and data analysis of the array were obtained with a colorimetric-based workstation...
  90. pmc Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patterns
    Erin M Smith
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 137:2006-12. 2007
    ..e. biotinylation of histones...
  91. ncbi A novel method for monitoring surface membrane trafficking on hippocampal acute slice preparation
    Judith Thomas-Crusells
    Program for Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Viikki Biocenter, FIN 00014, Helsinki, Finland
    J Neurosci Methods 125:159-66. 2003
    ..Here, we present a method for cell surface protein biotinylation in the acute hippocampal slice, the most commonly used preparation for electrophysiological recordings...
  92. ncbi Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells
    Olga Vagin
    Department of Physiology, School of Medicine at UCLA, Los Angeles, California 90073, USA
    J Biol Chem 280:43159-67. 2005
    ....
  93. ncbi Evaluation of two novel tag-based labelling technologies for site-specific modification of proteins
    Aline Tirat
    Novartis Institutes for BioMedical Research, Discovery Technologies, CH 4056 Basel, Switzerland
    Int J Biol Macromol 39:66-76. 2006
    ..Secondly, we tested in vivo and in vitro site-directed biotinylation with two different tags, consisting of either 15 (AviTag) or 72 amino acids (BioEase tag), which serve as a ..
  94. ncbi Ubiquitination and endocytosis of cell adhesion molecule DM-GRASP regulate its cell surface presence and affect its role for axon navigation
    Karsten Thelen
    Department of Developmental Neurobiology, University of Heidelberg, D 69120 Heidelberg, Germany
    J Biol Chem 283:32792-801. 2008
    ..Together, our data show that ubiquitination and endocytosis of DM-GRASP in concert regulate its cell surface concentration, which is crucial for its function in axon navigation...
  95. ncbi Covalent modification of an exposed surface turn alters the global conformation of the biotin carrier domain of Escherichia coli acetyl-CoA carboxylase
    A Chapman-Smith
    Department of Biochemistry, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 272:26017-22. 1997
    ..These data indicate that a conformational change accompanies biotinylation of the biotin domain...
  96. ncbi K8 and K12 are biotinylated in human histone H4
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Eur J Biochem 271:2257-63. 2004
    ..g. acetylation, methylation, and biotinylation. We sought to identify amino-acid residues that are biotinylated in histone H4, and to determine whether ..
  97. pmc Biotinylation of histones represses transposable elements in human and mouse cells and cell lines and in Drosophila melanogaster
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
    J Nutr 138:2316-22. 2008
    ..Collectively, this study reveals a novel diet-dependent epigenetic mechanism that could affect cancer risk...
  98. pmc Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3
    Keyna Kobza
    Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    BMB Rep 41:310-5. 2008
    ..This study tested the hypothesis that BirA ligase catalyzes the biotinylation of eukaryotic histones...
  99. pmc Lysine residues in N-terminal and C-terminal regions of human histone H2A are targets for biotinylation by biotinidase
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 68583 0806, USA
    J Nutr Biochem 17:225-33. 2006
    ..The primary aim of this study was to identify biotinylation sites in histone H2A and its variant H2AX...
  100. pmc Comparison of the backbone dynamics of the apo- and holo-carboxy-terminal domain of the biotin carboxyl carrier subunit of Escherichia coli acetyl-CoA carboxylase
    X Yao
    Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 21250, USA
    Protein Sci 8:307-17. 1999
    ..structures provided evidence for small, localized conformational changes in the biotin-binding region upon biotinylation of the protein. These structural changes may be important for regulating specific protein-protein interactions...
  101. ncbi Bacteriophage lambda surface display of a bacterial biotin acceptor domain reveals the minimal peptide size required for biotinylation
    J Stolz
    Friedrich Alexander Universitat Erlangen Nurnberg, Lehrstuhl Botanik II, Erlangen, Germany
    FEBS Lett 440:213-7. 1998
    ..displayed on bacteriophage lambda heads to determine the minimal sequence length that is necessary to effect biotinylation in vivo...

Research Grants66

  1. 5-HT transporter function: Interaction of hormones and antidepressants
    Alan Frazer; Fiscal Year: 2013
    ..In addition, to examine possible mechanisms underlying the hormonal effects: (1) biotinylation studies on synaptosomal membranes will be carried out to examine the plasma membrane distribution of the SERT ..
  2. GILZ Regulation of ENaC
    David Pearce; Fiscal Year: 2012
    ..the effects of GILZ1 and GILZIPs (particularly SGK1, Nedd4- 2 and Raf-1) on ENaC surface expression using biotinylation in cultured kidney epithelial cells;examine the effects of GILZ1 and GILZIPs on ENaC proteolytic processing;..
  3. Novel histone biotinylation sites and relationships to other epigenetic marks
    JANOS contact ZEMPLENI; Fiscal Year: 2010
    Histones are known to be modified by covalent binding of the vitamin biotin Histone biotinylation is unique among histone modifications in that a metabolic co-factor also functions as a chromatin mark...
  4. Presynaptic NMDAR Regulation of Substance P Release in the RVM After Injury
    UCHECHUKWU PATRICK MADUKA; Fiscal Year: 2013
    ..real time PCR and western blotting with subunit specific antibodies, phospho-specific antibodies and biotinylation probes...
  5. 5-HT transporter function: Interaction of antidepressants and hormones
    Alan Frazer; Fiscal Year: 2013
    ..hormonal effects, experiments are planned to study the plasma membrane distribution of the SERT using biotinylation studies;to examine the role of specific ER subtypes;and to study possible involvement of brain-derived ..
  6. SODIUM ENTRY INTO AMILORIDE-SENSITIVE EPITHELIA
    Catherine M Fuller; Fiscal Year: 2013
    ..In addition, we will employ surface biotinylation, surface chemiluminescence, co-immunoprecipitation analysis, and macromolecular assembly assays, to verify ..
  7. Novel Ca receptor signaling pathways for control of renal ion transport
    Richard Tyler Miller; Fiscal Year: 2012
    ..Define the mechanism by which the CaR activates WNK1 and WNK4. Cell surface expression (biotinylation) and channel density (whole cell patch) will be used as measures of CaR signaling through the WNK kinases in ..
  8. Synthetic Fabs for Crystallization of Chemokine:Receptor Complexes
    Tracy M Handel; Fiscal Year: 2013
    ..them into stable membrane mimetics called nanodiscs, immobilize the nanodisc particles through site specific biotinylation, and to make fluorescent chemokines for use in a robust screen to generate Fabs against any chemokine:receptor ..
  9. Defining the thyroid hormone receptor Cistrome in vivo
    Anthony N Hollenberg; Fiscal Year: 2012
    ..In the second aim, the biotinylation peptide will be knocked in to the endogenous TR21 genomic locus to allow for the development of a mouse model ..
  10. Vascular Smooth Muscle Function in Pulmonary Hypertension
    Nikki L Jernigan; Fiscal Year: 2013
    ..ASIC1 trafficking to the membrane through increased RhoA-mediated actin polymerization by use of cell surface biotinylation assays, [Ca2+]i imaging, and live-cell confocal imaging of a fluorescently- labeled ASIC1 protein...
  11. Junctional exocytosis and breakdown of the intestinal barrier in inflammation
    ANDREI IVANOVICH IVANOV; Fiscal Year: 2013
    ..Vesicle fusion machinery will be analyzed by a combination of biochemical (biotinylation, immunoprecipitation, affinity chromatography), immunocytochemical and genetic (siRNA knock-down of SNARE ..
  12. Stress, Depression, Serotonin, and Plasticity of Excitatory Transmission
    Scott M Thompson; Fiscal Year: 2013
    ..such as transfection with constitutively active and dominant negative constructs, western blotting, and biotinylation assays, to investigate the actions of 5-HT1B receptors in hippocampal slices taken from control animals and ..
  13. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2010
    ..abundance on the kinetics of restitution of cellular polarity will be explored using immunohistochemistry and biotinylation studies to track movement of displaced NaK ATPase following injury and during recovery...
  14. REGULATION OF MEMBRANE EXCITABILITY
    Kurt G Beam; Fiscal Year: 2012
    ..that neighbor functionally important sites within the DHPR by exploiting both proteomics and metabolic biotinylation. Accomplishing these specific aims should reveal essential new information about a basic muscle function, EC ..
  15. Modulation of MUC1 Mucin Trafficking
    Rebecca P Hughey; Fiscal Year: 2010
    ..We will use a combination of metabolic labeling, surface biotinylation and immunoblotting of endogenous EGFR and MUC1 and recombinant mutants in normal and glycosylation-defective ..
  16. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2010
    ..B) Surface biotinylation of BK channels in BAEC to determine whether BK channel activation involves the trafficking of BK channels from ..
  17. The Role of Ubiquitin in Sodium Chloride Co-Transporter Regulation
    BENJAMIN S KO; Fiscal Year: 2012
    ..will be established by measuring NCC activity via radiotracer uptake, NCC cell surface expression via biotinylation and immunofluorescence, and ubiquitination via immunoprecipitation and immunoblotting in cells gene-silenced ..
  18. Enteropathogenic E. coli effector proteins in intestinal tight junction recovery
    LILA GOLLOGLY; Fiscal Year: 2013
    ..b>Biotinylation assays will be used to determine the rate of TJ protein recycling after infection with EPEC WT or DespG1/2...
  19. Neural Plasticity and Inflammatory Pain
    Clifford J Woolf; Fiscal Year: 2013
    ..are expressed by purified dorsal root ganglion (DRG) neuronal membrane preparations using flow cytometry and biotinylation to label membrane proteins, affinity purification, trypsinization and liquid chromatography/mass spectrometry ..
  20. Ca2+ Waves in Hepatocytes: Mechanisms and Effects
    Michael H Nathanson; Fiscal Year: 2013
    ..of targeting and insertion of native transporters will be assessed in hepatocytes using immunoblot and biotinylation, and targeting and insertion of fluorescently tagged transporters will be assessed in liver cell lines using ..
  21. Vascular control by K+ channel trafficking
    Jonathan H Jaggar; Fiscal Year: 2013
    ..Methods used to test these hypotheses will include arterial biotinylation, FRET, RNAi, co-IP, immunofluorescence, patch-clamp electrophysiology, membrane potential recording, ..
  22. Cellular Protein Involved in Trafficking of HIV-1
    Carol A Carter; Fiscal Year: 2013
    ..assessed using bimolecular fluorescence complementation, immunoprecipitation, immuno-electron microscopy and biotinylation assays. Direct interactions will be checked using protein- protein interaction assays...
  23. Novel Approaches to Access GPIs and GPI-Anchored Proteins for the Study of GPI An
    Zhongwu Guo; Fiscal Year: 2012
    ..and all nascent proteins destined for GPI anchorage, their reaction with biotinylated GPIs will result in the biotinylation of all GPI-anchored proteins...
  24. Mints: Adaptor Proteins Coupling APP of Alzheimer's Disease to the Synapse
    Angela Ho; Fiscal Year: 2010
    ..We can now directly study: (1) membrane protein targeting by surface biotinylation of cell membranes, and morphologically examine the expression and cellular distribution of proteins that Mints ..
  25. Capture of Ubiquitin Conjugation and Deconjugation Enzyme Substrates
    Robert Cohen; Fiscal Year: 2010
    ..In Aim 1, modified biotin ligase and biotin acceptor peptide constructs will be designed to allow biotinylation only when these two components are brought together by interaction of an enzyme (E3 or DUB) with its substrate...
  26. Defining the Epigenetic Architecture Associated with Early-Life Stress
    Zhaolan Zhou; Fiscal Year: 2013
    ..in a spatially and temporally controlled manner;while the other line carries an endogenous MBP tagged with a biotinylation signal sequence...
  27. Tumor exosomes as agents of genetic change
    XANDRA OWENS BREAKEFIELD; Fiscal Year: 2011
    ..The advantage of this biotinylation approach is that it allows the imaging of brain tumors with different modalities including Magnetic Resonance (..
  28. A Proteomic and Biochemical Study of HIV-1 Nucleoprotein Complexes
    MICHAEL A BELSHAN; Fiscal Year: 2013
    ..Second, we will affinity purify HIV-1 protein complexes using a novel in vivo biotinylation method...
  29. SODIUM ENTRY INTO AMILORIDE-SENSITIVE EPITHELIA
    Dale Benos; Fiscal Year: 2009
    ..In addition, we will employ surface biotinylation and co-immunoprecipitation analysis to verify subunit interactions...
  30. Efficacy of Activated Protein C in Ventilator Associated Lung Injury
    James H Finigan; Fiscal Year: 2011
    ..Using measures of endothelial resistance, protein biochemistry, siRNA, RT-PCR, confocal microscopy, biotinylation and Western blotting will define the role of EPCR in cyclic stress- and APC-induced signaling and cytoskeletal ..
  31. Cx43 Hemichannels: Gating, Modification and Function
    Michael V L Bennett; Fiscal Year: 2011
    ..include time lapse recording of dye uptake, recording of single channel activity, isolation of surface Cx43 by biotinylation/ NeutrAvidin pull down, Western blot analysis and site directed mutagenesis...
  32. Cellular Protein Involved in Trafficking of HIV-1
    Carol A Carter; Fiscal Year: 2012
    ..assessed using bimolecular fluorescence complementation, immunoprecipitation, immuno-electron microscopy and biotinylation assays. Direct interactions will be checked using protein- protein interaction assays...
  33. TMEM16A Channel Stoichiometry and Subunit Interaction
    Jason Tien; Fiscal Year: 2013
    ..Using electrophysiology, immunoprecipitation, and biotinylation, the proportion of CaCC complexes that contain only subunits with one tag but not the other will be observed...
  34. Fibroblast Growth Factor Homologous Factors: Modulation of L-type Calcium Channel
    JESSICA AMENTA HENNESSEY; Fiscal Year: 2013
    ..2 current, utilizing co-immunoprecipitation, surface biotinylation, and in vitro binding asays...
  35. Alcohol and Developing Neuronal Circuits
    CARLOS F VALENZUELA; Fiscal Year: 2013
    ..also determine whether EtOH exposure decreases plasma membrane expression of L-VGCC subunits using a surface biotinylation assay...
  36. Multiple Autoantigens, Multiple Epitopes of Type 1 Diabetes
    Marian J Rewers; Fiscal Year: 2013
    ..fully characterize the MSD insulin autoantibody assay and to test modifying N-Hydroxy-succinamide labeling and biotinylation of proinsulin to further enhance the assay...
  37. Oct4 and epigenetic regulation of stem cell pluripotency
    Jianlong Wang; Fiscal Year: 2013
    ..To dissect the molecular basis for the pluripotency of ESCs, we have developed an in vivo biotinylation strategy for affinity purification of protein complexes (dubbed bioSAIP) and chromatin immunoprecipitation (..
  38. Functional Genetics of the Neuronal Sodium Channel Gene SCN8A
    Miriam H Meisler; Fiscal Year: 2013
    ..We will carry out in vivo biotinylation of an Avi-Tagged Nav1...
  39. Molecular UBM and MRI of Vascular Development
    Daniel H Turnbull; Fiscal Year: 2013
    ..Several reports have recently demonstrated that biotinylation of cell surfaces can be achieved, allowing cell-targeted imaging with avidin-conjugated contrast agents, which ..
  40. Tau and Glutamatergic Synapses in Frontotemporal Dementia
    Brian A Warmus; Fiscal Year: 2013
    ..is decreased in ventral striatum of hTauV337M mice, we will measure surface AMPAR levels at the synapse by biotinylation and biochemical fractionation of ventral striatum...
  41. Glutamate Receptor Desensitization and its Modulation
    KATHRYN MCKAY PARTIN; Fiscal Year: 2012
    ..energy transfer, a new methodology of probing tertiary and quaternary protein complexes using metabolic biotinylation, and computational modeling: 1...
  42. Mechanistic studies of fibronectin peptide P12: a co-factor of PDGF-BB
    RICHARD AUGUST CLARK; Fiscal Year: 2013
    ..For this aim, PDGF/P12 induced PDGFR internalization will be measured by reversible biotinylation. Then, Inhibitors for different internalization pathways will be employed to determine the pathway the P12 ..
  43. BIN1 is a mediator and marker of cardiac reserve in heart failure.
    Tingting Hong; Fiscal Year: 2013
    ..2 in failing and non-failing human cardiomyocytes. In adult mouse cardiomyocyte models, surface biotinylation and live-cell calcium imaging will be used to study Cav1...
  44. BIN1 is a mediator and marker of cardiac reserve in heart failure.
    Tingting Hong; Fiscal Year: 2013
    ..2 in failing and non-failing human cardiomyocytes. In adult mouse cardiomyocyte models, surface biotinylation and live-cell calcium imaging will be used to study Cav1...
  45. Defense against Alzheimer??s-associated A?? oligomers by CNS insulin signaling
    JASON ERNEST PITT; Fiscal Year: 2011
    ..Intracellular uptake and extracellular re- lease of proteins will be distinguished using reversible surface biotinylation with co-immunoprecipitation...
  46. Membrane Processes Mediating K Secretion
    Lawrence G Palmer; Fiscal Year: 2013
    ..the total abundance of channels using immunoblotting, and the amount of protein in the apical membrane using a biotinylation approach for labeling proteins at the cell surface...
  47. The Role of TRIP8b in Neuronal HCN Channel Trafficking
    ALAN SETH LEWIS; Fiscal Year: 2012
    ..We will use single-cell RT-PCR, biotinylation, and viral delivery of TRIPSb splice isoforms to determine how TRIPSb splicing impacts h channel surface ..
  48. (PQB6) Accurate Comparative Genome-wide Analysis of Primary Tumors and Metastases
    David Wotton; Fiscal Year: 2013
    ..2) Generate and test a genetically engineered mouse model in which expression of a nuclear envelope biotinylation cassette can be induced by the Cre recombinase...
  49. FIBRONECTIN RECEPTOR IN CANDIDA TROPICALIS
    MARGARET HOSTETTER; Fiscal Year: 2003
    ..tropicalis on the FN molecule. In Specific Aim Three, we will use surface biotinylation and immunoprecipitation to characterize other surface proteins in C...
  50. In vivo biotinylation for analysis of nuclear and protein dynamics
    TERESA A NICOLSON; Fiscal Year: 2013
    ..Both techniques involve tissue-specific biotinylation of nuclei or proteins that occurs in vivo before processing of samples, and both techniques offer several ..
  51. Trypanosome surface protein targeting and function at the host-parasite interface
    Michelle M Shimogawa; Fiscal Year: 2013
    ..By developing a novel strategy for isolation of intact membrane-enclosed flagella, combined with surface biotinylation and shotgun proteomics, we have conducted the first proteomic analysis of the flagellar membrane from ..
  52. EXOCYTIC INSERTION OF POTASSIUM CHANNELS IN MEMBRANES
    William Moran; Fiscal Year: 2001
    ..an electrical resistance method to determine the effect of alanine on enterocyte volume; and 5) cell surface biotinylation and Western blot analysis to determine the relative number of apical membrane K channels...
  53. REPAIR OF HAIR BUNDLES IN SEA ANEMONES
    GLEN WATSON; Fiscal Year: 1999
    ..Any repair proteins losing biological activity as a result of biotinylation will be used as antigens to generate polyclonal antibodies. The antibodies will be used in immunocytochemistry...
  54. Role of Myosin VI in CFTR Endocytosis in the Intestine
    Nadia Ameen; Fiscal Year: 2007
    ..We will employ cell biological approaches including surface biotinylation, Western Blotting, immunofluorescece and immunoelectron microscopic localization in all animal and cell ..
  55. Retinyl Ester Binding Proteins and the Visual Cycle
    Robert Rando; Fiscal Year: 2007
    ..Specific affinity biotinylation methods, which proved to be successful in the identification of mRPE65 as a tRE binding protein, will be ..
  56. Membrane Proteins of S. mansoni as Vaccine Candidates
    Donald Harn; Fiscal Year: 2002
    ..a glucose transporter protein (SGTP4) that was definitely demonstrated by immunolocalization and surface biotinylation to be exposed at the host/parasite interface in the worm tegument...
  57. STRUCTURE AND ORGANIZATION OF THE PHOTOSYSTEM I COMPLEX
    Parag Chitnis; Fiscal Year: 1999
    ..NHS- biotinylation, protease accessibility, and chemical cross-linking studies will be used...
  58. CONFORMATIONAL CHANGES ASSOCIATED WITH K+ CHANNEL GATING
    ROBERT BLAUSTEIN; Fiscal Year: 2000
    ..to introduce single cysteines into the K+ channel sequence, creating potential targets for cysteine-specific biotinylation. Under electrophysiologic recording, oocytes with open channels will be exposed to a biotinylating reagent and ..
  59. TRANSPORT OF AN E COLI VIRULENCE FACTOR--THE K1 CAPSULE
    Richard Silver; Fiscal Year: 2000
    ..face of the cytoplasmic membrane during the translocation process? These studies will include protease and biotinylation accessibility in oriented membrane vesicles...
  60. ACTIVATION OF CFTR MUTANTS IN VIVO FOR CF THERAPY
    MITCHELL DRUMM; Fiscal Year: 1999
    ..and increasing channel number, the quantity of CFTR in the plasma membrane will be measured by cell-surface biotinylation before and after stimulation...
  61. DESENSITIZATION AND DOWN REGULATION OF CALCIUM RECEPTORS
    Dolores Shoback; Fiscal Year: 2002
    ..responses after treating cells with agonists and by quantifying internalization of CaRs using a surface biotinylation assay...
  62. PHOSPHOINOSITIDES IN NORMAL AND ABNORMAL HUMAN RED CELLS
    George Dale; Fiscal Year: 1992
    ..developed procedure for the isolation of senescent erythrocytes will be utilized; this procedure involves the biotinylation of rabbit erythrocytes...
  63. Physiologic membrane traffic regulation: intestinal CFTR
    Nadia Ameen; Fiscal Year: 2006
    ..Functional biochemical techniques such biotinylation/immunoprecipitation will complement morphologic studied to investigate a role for membrane traffic...
  64. Chimeric CD80-SA as a Novel Cancer Vaccine
    Rolf Huseby; Fiscal Year: 2006
    ..ProtEx involves the generation of chimeric proteins with core streptavidin, biotinylation of the cell surface, and decoration with chimeric proteins...
  65. MOLECULAR REGULATION AND FUNCTION OF NA/H EXCHANGER-2
    Chung Ming Tse; Fiscal Year: 1999
    ..two dimensional phosphopeptide mapping with separating the cell surface and intracellular forms of NHE2 using biotinylation; (2) to determine whether the increase in V max of NHE2 by growth factors/protein kinases is due to an ..
  66. Melatonin Regulation of MT2 Melatonin Receptors
    MATTHEW GERDIN; Fiscal Year: 2003
    ..negatives of G protein receptor kinases (GRK), arrestins, and dynamin, confocal microscopic and cell surface biotinylation experiments will examine the process of CHO-MT2 receptor internalization...