phase contrast microscopy

Summary

Summary: A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.

Top Publications

  1. ncbi Immunocytochemical comparison of cultured normal epithelial prostatic cells with prostatic tissue sections
    O Cussenot
    Département de Recherche en Urologie, Hopital Saint Louis, Paris, France
    Exp Cell Res 214:83-92. 1994
  2. ncbi The effect of an age-related hearing loss gene (Ahl) on noise-induced hearing loss and cochlear damage from low-frequency noise
    Gary W Harding
    Department of Otolaryngology, Box 8115, Washington University School of Medicine, St Louis, MO 63110, USA
    Hear Res 204:90-100. 2005
  3. ncbi Influence of channel width on alignment of smooth muscle cells by high-aspect-ratio microfabricated elastomeric cell culture scaffolds
    John D Glawe
    Biomedical Engineering Program, Louisiana Tech University, Ruston, LA 71272, USA
    J Biomed Mater Res A 75:106-14. 2005
  4. ncbi Structural changes in epidermal scale and appendages as indicators of defective TGM1 activity
    Robert H Rice
    Department of Environmental Toxicology, University of California, One Shields Avenue, Davis, CA 95616 8588, USA
    Arch Dermatol Res 297:127-33. 2005
  5. ncbi Embryonic stem cells and cardiomyocyte differentiation: phenotypic and molecular analyses
    Hong Wei
    Laboratory of Cardiovascular Science, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    J Cell Mol Med 9:804-17. 2005
  6. ncbi Medical phase contrast x-ray imaging: current status and future prospects
    R A Lewis
    Monash Centre for Synchrotron Science and School of Physics and Materials Engineering, Monash University, Victoria 3800, Australia
    Phys Med Biol 49:3573-83. 2004
  7. ncbi Spectral-domain phase microscopy
    Michael A Choma
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Opt Lett 30:1162-4. 2005
  8. doi Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy, and optical tweezers
    Lingbo Kong
    Department of Physics, East Carolina University, Greenville, North Carolina 27858 4353, USA
    Anal Chem 82:3840-7. 2010
  9. ncbi Vibrational phase contrast microscopy by use of coherent anti-Stokes Raman scattering
    M Jurna
    Optical Sciences Group, MESA Institute for Nanotechnology, Faculty of Science and Technology TNW, University of Twente, The Netherlands
    Phys Rev Lett 103:043905. 2009
  10. ncbi Proliferative response of corneal endothelial cells from young and older donors
    Cheng Zhu
    Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Invest Ophthalmol Vis Sci 45:1743-51. 2004

Research Grants

  1. LISTERIA USES HOST CELL ACTIN TO SPREAD CELL TO CELL
    Frederick Southwick; Fiscal Year: 1993
  2. GENETIC DETERMINANTS OF VIRULENCE IN VIBRIO CHOLERAE
    Ronald Taylor; Fiscal Year: 2007
  3. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2007
  4. LIPID METABOLISM IN FAT CELLS
    Wen Guo; Fiscal Year: 2005
  5. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 2006
  6. PHARMACOLOGIC PREVENTION OF NEURONAL DEATH AFTER INJURY
    Keith Rich; Fiscal Year: 1993
  7. VISUAL NEURONS: DEVELOPMENT AND REGENERATION IN VITRO
    Ruben Adler; Fiscal Year: 1990
  8. Functional Analysis of Mitotic Checkpoint Gene Bub1b
    Rakesh Goorha; Fiscal Year: 2005
  9. CYTOMIMETIC ORGANIC CHEMISTRY
    FREDRIC MENGER; Fiscal Year: 2007
  10. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 1992

Detail Information

Publications193 found, 100 shown here

  1. ncbi Immunocytochemical comparison of cultured normal epithelial prostatic cells with prostatic tissue sections
    O Cussenot
    Département de Recherche en Urologie, Hopital Saint Louis, Paris, France
    Exp Cell Res 214:83-92. 1994
    ....
  2. ncbi The effect of an age-related hearing loss gene (Ahl) on noise-induced hearing loss and cochlear damage from low-frequency noise
    Gary W Harding
    Department of Otolaryngology, Box 8115, Washington University School of Medicine, St Louis, MO 63110, USA
    Hear Res 204:90-100. 2005
    ..However, this appeared not to be the case for PTS. The Ahl gene appears to play a role in susceptibility to NIHL but, other genes as well as systemic and local factors must also be involved...
  3. ncbi Influence of channel width on alignment of smooth muscle cells by high-aspect-ratio microfabricated elastomeric cell culture scaffolds
    John D Glawe
    Biomedical Engineering Program, Louisiana Tech University, Ruston, LA 71272, USA
    J Biomed Mater Res A 75:106-14. 2005
    ....
  4. ncbi Structural changes in epidermal scale and appendages as indicators of defective TGM1 activity
    Robert H Rice
    Department of Environmental Toxicology, University of California, One Shields Avenue, Davis, CA 95616 8588, USA
    Arch Dermatol Res 297:127-33. 2005
    ..Both epidermal scale and nail from the index case lacked the prominent cell borders that were visible by phase contrast microscopy after detergent extraction of control samples...
  5. ncbi Embryonic stem cells and cardiomyocyte differentiation: phenotypic and molecular analyses
    Hong Wei
    Laboratory of Cardiovascular Science, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    J Cell Mol Med 9:804-17. 2005
    ..With these issues in mind, this review will focus on the differentiation of pluripotent embryonic stem cells into cardiomyocytes as a developmental model, and the possible use of ES cell-derived cardiomyocytes as source of donor cells...
  6. ncbi Medical phase contrast x-ray imaging: current status and future prospects
    R A Lewis
    Monash Centre for Synchrotron Science and School of Physics and Materials Engineering, Monash University, Victoria 3800, Australia
    Phys Med Biol 49:3573-83. 2004
    ..The current state of the art is reviewed...
  7. ncbi Spectral-domain phase microscopy
    Michael A Choma
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Opt Lett 30:1162-4. 2005
    ..A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos...
  8. doi Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy, and optical tweezers
    Lingbo Kong
    Department of Physics, East Carolina University, Greenville, North Carolina 27858 4353, USA
    Anal Chem 82:3840-7. 2010
    We present a methodology that combines external phase contrast microscopy, Raman spectroscopy, and optical tweezers to monitor a variety of changes during the germination of single Bacillus cereus spores in both nutrient (l-alanine) and ..
  9. ncbi Vibrational phase contrast microscopy by use of coherent anti-Stokes Raman scattering
    M Jurna
    Optical Sciences Group, MESA Institute for Nanotechnology, Faculty of Science and Technology TNW, University of Twente, The Netherlands
    Phys Rev Lett 103:043905. 2009
    ..The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933.
  10. ncbi Proliferative response of corneal endothelial cells from young and older donors
    Cheng Zhu
    Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Invest Ophthalmol Vis Sci 45:1743-51. 2004
    ....
  11. ncbi Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
    N Q Balaban
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Nat Cell Biol 3:466-72. 2001
    ..The results put clear constraints on the possible molecular mechanisms for the mechanosensory response of focal adhesions to applied force...
  12. ncbi Spectral-domain optical coherence phase microscopy for quantitative phase-contrast imaging
    Chulmin Joo
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Opt Lett 30:2131-3. 2005
    ..We demonstrate subnanometer level path-length sensitivity and present images obtained on reflection from a known phase object and human epithelial cheek cells...
  13. ncbi Single particle analysis based on Zernike phase contrast transmission electron microscopy
    Radostin Danev
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji cho, Okazaki, Aichi 444 8787, Japan
    J Struct Biol 161:211-8. 2008
    ..The same resolution, about 12A, was achieved by both imaging methods. The reconstruction based on Zernike phase contrast data required about 30% fewer particles. The advantages and prospects of each technique are discussed...
  14. pmc Field-based angle-resolved light-scattering study of single live cells
    Wonshik Choi
    G R Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Opt Lett 33:1596-8. 2008
    ....
  15. ncbi Transmission electron microscopy with Zernike phase plate
    R Danev
    National Institute for Physiological Sciences, Okazaki National Research Institutes, Japan
    Ultramicroscopy 88:243-52. 2001
    ..In order to make the technique user-friendly a number of improvements have to be made, and are discussed in terms of the current level of technology and instrumentation...
  16. pmc Intact carboxysomes in a cyanobacterial cell visualized by hilbert differential contrast transmission electron microscopy
    Yasuko Kaneko
    Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo okubo, Sakura ku, Saitama 338 8570, Japan
    J Bacteriol 188:805-8. 2006
    ..The carboxysome was surrounded by a 5- to 6-nm-thick monolayer shell which consisted of orderly arrays of globular particles...
  17. pmc Two components of actin-based retrograde flow in sea urchin coelomocytes
    J H Henson
    Department of Biology, Dickinson College, Carlisle, Pennsylvania 17013, USA
    Mol Biol Cell 10:4075-90. 1999
    ..We propose a "push-pull" mechanism in which actin polymerization drives flow at the cell periphery, whereas myosin II provides the tension on the actin cytoskeleton necessary for flow in the cell interior...
  18. pmc Tracking retrograde flow in keratocytes: news from the front
    Pascal Vallotton
    Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    Mol Biol Cell 16:1223-31. 2005
    ..Our findings support the universality of the flow phenomenon and indicate that the maintenance of keratocyte shape during locomotion depends on the regulation of both retrograde flow and actin polymerization...
  19. pmc Orientational order of the lamellipodial actin network as demonstrated in living motile cells
    Alexander B Verkhovsky
    Laboratory of Cell Biophysics and Biomechanics, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland
    Mol Biol Cell 14:4667-75. 2003
    ..Here, we use an enhanced phase contrast microscopy technique to visualize an apparent long-range diagonal actin meshwork in the advancing lamellipodia of ..
  20. ncbi Biometry of frozen-thawed sperm from eight breeds of Indian buffaloes (Bubalus bubalis)
    R A K Aggarwal
    National Bureau of Animal Genetic Resources, Karnal, India
    Theriogenology 68:682-6. 2007
    ..Multivariate analysis and clustering put six breeds (Surti, Tarai, Mehsana, Jaffrabadi, Bhadawari and Nili-Ravi) in one cluster, whereas Murrah and Pandharpuri appeared as separate entities...
  21. ncbi Posterior capsule opacification-like changes in rat lens explants cultured with TGFbeta and FGF: effects of cell coverage and regional differences
    Joel G Symonds
    Discipline of Anatomy and Histology and Institute for Biomedical Research F13, University of Sydney, Sydney, NSW 2006, Australia
    Exp Eye Res 82:693-9. 2006
    ..Such overall resilience to variations in conditions may contribute to the insidious nature of PCO, while factors related to observed early differences between groups may contribute to PCO pleiomorphism...
  22. ncbi Synchrotron radiation in radiology: radiology techniques based on synchrotron sources
    Reto Meuli
    Service de radiodiagnostic et radiologie interventionnelle, CHUV, 1011 Lausanne, Switzerland
    Eur Radiol 14:1550-60. 2004
    ....
  23. pmc In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood
    Ki Soo Park
    Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health and BK21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    J Vet Sci 7:343-8. 2006
    ..Therefore, it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy...
  24. ncbi Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy
    Björn Kemper
    University of Muenster, Laboratory of Biophysics, Robert Koch Str 45, D 48129 Muenster, Germany
    J Biomed Opt 12:054009. 2007
    ..Results obtained from human pancreas and liver tumor cells show that the integral cellular refractive index decreases with increasing cell radius...
  25. ncbi Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging
    Patrik Langehanenberg
    Center for Biomedical Optics and Photonics, University Medical Center of Muenster, Muenster, Germany
    Appl Opt 47:D176-82. 2008
    ....
  26. ncbi Bone nodule formation via in vitro differentiation of murine embryonic stem cells
    Sarah K Bronson
    Department of Cellular and Molecular Physiology, Penn State College of Medicine, Milton S Hershey Medical Center, H166, 500, University Drive, Hershey, Pennsylvania 17033 0850, USA
    Methods Enzymol 365:241-51. 2003
  27. ncbi Differentiation of mouse embryonic stem cells into pancreatic and hepatic cells
    Gabriela Kania
    In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research, Corrensstr 3, Gatersleben, D 06466, Germany
    Methods Enzymol 365:287-303. 2003
    ..Further, a histotypic "spinner" culture system was introduced to generate mature insulin- and albumin-producing cells at high efficiency...
  28. ncbi Serine proteases mediate apoptosis-like cell death and phagocytosis under caspase-inhibiting conditions
    L Egger
    Institute of Molecular Medicine and Cell Research, Albert Ludwigs University Freiburg, Zentrale Klinische Forschung, Breisacherstrasse 66, D 79106 Freiburg, Germany
    Cell Death Differ 10:1188-203. 2003
    ..Thus, despite caspase inhibition or basal caspase activities, cells can still be phagocytosed and killed in an apoptosis-like fashion by a serine protease-mediated mechanism that damages the mitochondrial membrane...
  29. ncbi Fibronectin fragments active in chondrocytic chondrolysis can be chemically cross-linked to the alpha5 integrin receptor subunit
    G A Homandberg
    Department of Biochemistry, Rush Medical College at Rush Presbyterian St Luke s Medical Center, 1653 West Congress Parkway, Chicago, IL 60612 3864, USA
    Osteoarthritis Cartilage 10:938-49. 2002
    ....
  30. ncbi Roles of ionotropic glutamate receptors in early developing neurons derived from the P19 mouse cell line
    Yi Hsuan Lee
    Department of Physiology, Taipei Medical University, Taipei, Taiwan, ROC
    J Biomed Sci 10:199-207. 2003
    ..Therefore, AMPA and KA receptors might mediate neurotrophic functions to facilitate neurotrophic factor signaling to protect neurons against hypoxic insult in early neuronal development...
  31. ncbi Stem cells (p63(+)) in keratinocyte cultures from human adult skin
    E Radu
    Department of Cellular and Molecular Medicine, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
    J Cell Mol Med 6:593-8. 2002
    ..The main step was the selection of cells able to rapidly adhere to human collagen type IV in vitro. Thus, an increased proportion of putative ESC of about 65% was obtained, as demonstrated by p63 expression...
  32. ncbi Endostatin induces autophagic cell death in EAhy926 human endothelial cells
    Y P Chau
    Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, Taipei, Taiwan, ROC
    Histol Histopathol 18:715-26. 2003
    ....
  33. ncbi Hemocyte-mediated phagocytosis and melanization in the mosquito Armigeres subalbatus following immune challenge by bacteria
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Cell Tissue Res 313:117-27. 2003
    ..These studies implicate hemocytes as the primary line of defense against bacteria...
  34. ncbi Identification and differential expression of novel human cervical cancer oncogene HCCR-2 in human cancers and its involvement in p53 stabilization
    Jesang Ko
    Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul 138 736, South Korea
    Oncogene 22:4679-89. 2003
    ..These results indicate that HCCR-2 probably represents a new oncogene that is related to tumorigenesis, functioning as a negative regulator of the p53 tumor suppressor...
  35. pmc Oogenesis in cultures derived from adult human ovaries
    Antonin Bukovsky
    Laboratory of Development, Differentiation and Cancer, Department of Obstetrics and Gynecology, The University of Tennessee Graduate School of Medicine, Knoxville, Tennessee, USA
    Reprod Biol Endocrinol 3:17. 2005
    ..In addition, other clinical applications aiming to utilize stem cells, and basic stem cell research as well, may employ totipotent embryonic stem cells developing from fertilized oocytes...
  36. ncbi Interleukin-15 increases myosin accretion in human skeletal myogenic cultures
    Paul S Furmanczyk
    University of Washington, School of Medicine, Seattle, WA 98195, USA
    Cell Biol Int 27:845-51. 2003
    ....
  37. ncbi Liver sinusoidal endothelial cells are the principal site for elimination of unfractionated heparin from the circulation
    Cristina Ionica Oie
    Center for Atherothrombotic Research in Tromsø, Department of Medicine, Institute of Clinical Medicine, University of Tromsø, N 9037 Tromsø, Norway
    Am J Physiol Gastrointest Liver Physiol 294:G520-8. 2008
    ..The results suggest a major role for LSECs in heparin elimination...
  38. ncbi Defined conditions for neural commitment and differentiation
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, UK
    Methods Enzymol 365:327-41. 2003
    ....
  39. ncbi Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds
    M W Hayman
    School of Biological and Biomedical Science, University of Durham, South Road, DH1 3LE, Durham, UK
    Biochem Biophys Res Commun 314:483-8. 2004
    ..These data suggest that the topography within the culture environment can significantly alter cell development and will therefore be an important feature when investigating the potential of human stem cells...
  40. ncbi Establishment of a corneal epithelial cell line spontaneously derived from human limbal cells
    Jingbo Liu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat Sen University, 54 Xian Lie Nan Road, Guangzhou, Guangdong 510060, China
    Exp Eye Res 84:599-609. 2007
    ..This cell line would be useful for studies of corneal epithelial biology and reconstructive corneal tissue engineering...
  41. ncbi Plasmodium yoelii: axenic development of the parasite mosquito stages
    Johanna M Porter-Kelley
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Exp Parasitol 112:99-108. 2006
    ..Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations...
  42. ncbi Fibronectin and cell attachment to cell and protein resistant polyelectrolyte surfaces
    Scott G Olenych
    Department of Biological Science, The Florida State University, Tallahassee, Florida 32306, USA
    Biomacromolecules 6:3252-8. 2005
    ..Both cells and FN interacted much better with Nafion than with PAA-co-PAEDAPS in a micropatterned PEMU. These results indicate that A7r5 cell adhesion, spreading, and motility on PEMUs can be independent of FN binding to the surfaces...
  43. pmc Conjugated linoleic acid promotes human adipocyte insulin resistance through NFkappaB-dependent cytokine production
    Soonkyu Chung
    Department of Nutrition, University of North Carolina at Greensboro, Greensboro, North Carolina 27402 6170, USA
    J Biol Chem 280:38445-56. 2005
    ....
  44. ncbi Inhibition of the calcium- and voltage-dependent big conductance potassium channel ameliorates cisplatin-induced apoptosis in spiral ligament fibrocytes of the cochlea
    F Liang
    Department of Pathology and Laboratory Medicine, 165 Ashley Avenue, Suite 309, PO Box 250908, Charleston, SC 29425, USA
    Neuroscience 135:263-71. 2005
    ..These findings also point to the calcium- and voltage-dependent big conductance potassium channels as a potential pharmacological target for manipulating cisplatin ototoxicity...
  45. ncbi Reconstruction of 3D stacked-up structures by rat small hepatocytes on microporous membranes
    Ryo Sudo
    Center for Life Science and Technology, School of Fundamental Science and Technology, Keio University, Yokohama, Japan
    FASEB J 19:1695-7. 2005
    ..We conclude that highly differentiated 3D tissues, including functional BC, can be reconstructed by stacking up layers of SHs. This 3D stacked-up culture is useful for the reconstruction of tissue-engineered livers...
  46. ncbi Advanced glycation end-products attenuate human mesenchymal stem cells and prevent cognate differentiation into adipose tissue, cartilage, and bone
    Shinichiro Kume
    Department of Orthopaedic Surgery, Kurume University School of Medicine, Kurume, Japan
    J Bone Miner Res 20:1647-58. 2005
    ....
  47. pmc Phenotypic and functional characterization of adult brain neuropoiesis
    Bjorn Scheffler
    Departments of Neuroscience and Neurosurgery, University of Florida, McKnight Brain Institute, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:9353-8. 2005
    ....
  48. ncbi Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells
    Minseok S Kim
    Department of Biosystems, Korea Advanced Institute of Science and Technology KAIST, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Lab Chip 7:513-5. 2007
    ..The ESCD will contribute to the advance quality control of in vitro ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving...
  49. ncbi Analysis of leukocyte migration through monolayers of cultured endothelial cells
    Helen M McGettrick
    Division of Medical Sciences, Department of Physiology, The Medical School, The University of Birmingham, Birmingham, UK
    Methods Mol Biol 370:37-54. 2007
    ..In general, the filter-based assay has the highest throughput and greatest ease of use but yields less detailed information, whereas the flow-based assay is most difficult to set up but is most physiologically relevant...
  50. ncbi Beta ig-h3 induces keratinocyte differentiation via modulation of involucrin and transglutaminase expression through the integrin alpha3beta1 and the phosphatidylinositol 3-kinase/Akt signaling pathway
    Ju Eun Oh
    Department of Oral Biochemistry and Craniomaxillofacial Reconstructive Sciences, Dental Research Institute, and BK21 HLS, Seoul National University College of Dentistry, Seoul 110 749, Korea
    J Biol Chem 280:21629-37. 2005
    ..Lastly, it was observed that beta ig-h3-mediated keratinocyte differentiation was caused by promotion of cell adhesion and not by calcium regulation...
  51. ncbi Controlling mammalian cell interactions on patterned polyelectrolyte multilayer surfaces
    Michael C Berg
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Langmuir 20:1362-8. 2004
    ..Finally, we found that pattern geometry affected cytoskeletal protein organization. Well-formed focal adhesions and cell-spanning stress fibers were only found in cells on wider line patterns (at least 25 microm in width)...
  52. ncbi Keratinocytes display normal proliferation, survival and differentiation in conditional beta4-integrin knockout mice
    Karine Raymond
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Cell Sci 118:1045-60. 2005
    ..Our findings clearly demonstrate that, after birth, epidermal differentiation, proliferation and survival all proceed normally in the absence of alpha6beta4, provided that cell adhesion is not compromised...
  53. ncbi G2 cell cycle arrest, down-regulation of cyclin B, and induction of mitotic catastrophe by the flavoprotein inhibitor diphenyleneiodonium
    Robin M Scaife
    Laboratory for Cancer Medicine, West Australian Institute for Medical Research, Perth, Western Australia 6009, Australia
    Mol Cancer Ther 3:1229-37. 2004
    ..Hence, the antiproliferative activity of DPI reflects a novel inhibitory mechanism of cell cycle progression that can reverse spindle checkpoint-mediated cell cycle arrest...
  54. ncbi Cytoplasmic nuclear transfer of the actin-capping protein tropomodulin
    Kimi Y Kong
    Institute for Genetic Medicine and Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 279:30856-64. 2004
    ..These results suggest that, at least in myogenic cells, nuclear Tmod may be involved in the differentiation process...
  55. ncbi Use of a novel 3D culture model to elucidate the role of mesothelial cells, fibroblasts and extra-cellular matrices on adhesion and invasion of ovarian cancer cells to the omentum
    Hilary A Kenny
    Department of Obstetrics and Gynecology Section of Gynecologic Oncology, Center for Integrative Science, University of Chicago, Chicago, IL 60637, USA
    Int J Cancer 121:1463-72. 2007
    ....
  56. pmc A high-throughput microfluidic real-time gene expression living cell array
    Kevin R King
    Center for Engineering and Medicine and Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA
    Lab Chip 7:77-85. 2007
    ..By enabling efficient investigation of dynamic gene expression programs, this technology has the potential to make significant impacts in basic science, drug development, and clinical medicine...
  57. ncbi Thyroid hormone regulates the expression of alpha-internexin in neurons in culture
    David Sampson
    Division of Molecular Endocrinology, University College London Medical School, Mortimer Street, London W1N 8AA, UK
    Neuroreport 13:273-6. 2002
    ..This supports the hypothesis that maternal thyroid hormone directly regulates early neuronal differentiation...
  58. ncbi Histone deacetylases inhibitors as anti-angiogenic agents altering vascular endothelial growth factor signaling
    Christophe F Deroanne
    Research Center in Experimental Cancerology, University of Liege, Sart Tilman, B 4000 Liege, Belgium
    Oncogene 21:427-36. 2002
    ..These observations provide a conspicuous demonstration that HDAC inhibitors are potent anti-angiogenic factors altering VEGF signaling...
  59. ncbi Multiple fibroblast growth factors support growth of the ureteric bud but have different effects on branching morphogenesis
    J Qiao
    Department of Medicine, VASD HS and Cancer Center, University of California, San Diego, CA 92093 0693, USA
    Mech Dev 109:123-35. 2001
    ....
  60. ncbi The characterisation of human respiratory epithelial cells cultured on resorbable scaffolds: first steps towards a tissue engineered tracheal replacement
    Brian W Ziegelaar
    Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Germany
    Biomaterials 23:1425-38. 2002
    ..Furthermore, that UEA and PNA staining is a useful tool in the characterisation of cells cultured on biomaterials, therefore helpful in identifying biomaterials that are suitable for specific tissue engineering purposes...
  61. ncbi The role of surface wettability on hepatocyte adhesive interactions and function
    N Krasteva
    Institute of Biophysics, Bulgarian Academy of Sciences, Sofia
    J Biomater Sci Polym Ed 12:613-27. 2001
    ..It was concluded that the wettability of materials has a strong influence on the attachment and morphology of hepatocytes while the influence of surface properties on the functional activity of hepatocytes still remains to be elucidated...
  62. ncbi Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells
    E H Javazon
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Stem Cells 19:219-25. 2001
    ..The rat mMSCs differed from human mMSCs in that they regenerated RS cells in culture. Also, after low-density plating, colonies of rMSCs expanded into confluent cultures, whereas colonies of hMSCs did not...
  63. ncbi Protein kinase C alpha and gamma in N/N 1003A rabbit lens epithelial cell differentiation
    L M Wagner
    Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA
    Mol Vis 7:57-62. 2001
    ..METHODS: PKCalpha or PKCgamma was overexpressed in N/N 1003A lens epithelial cells for up to 7 days. Phase contrast microscopy was used to observe morphological changes associated with PKCalpha or PKCgamma overexpression...
  64. ncbi Differentiation of liver epithelial (stem-like) cells into hepatocytes induced by coculture with hepatic stellate cells
    Hirokazu Nagai
    Department of Biochemistry, Akita University School of Medicine, Hondo 1 1 1, Akita 010 8543, Japan
    Biochem Biophys Res Commun 293:1420-5. 2002
    ..Not only the extracellular matrices but also soluble factors, which are produced by hepatic stellate cells, induce this maturation, demonstrating the importance of the hepatic environment for hepatocyte differentiation...
  65. ncbi Ep-CAM transfection in thymic epithelial cell lines triggers the formation of dynamic actin-rich protrusions involved in the organization of epithelial cell layers
    J C Guillemot
    CNRS LNB, , Chemin de J Aiguier, Marseille 13009, France
    Histochem Cell Biol 116:371-8. 2001
    ..These results suggest that expression of Ep-CAM might balance the organizing capacity of cadherin molecules and may be participating in the formation of a dynamic stromal cell network in the thymus...
  66. pmc Protein tyrosine phosphatase phi regulates paxillin tyrosine phosphorylation and mediates colony-stimulating factor 1-induced morphological changes in macrophages
    F J Pixley
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 21:1795-809. 2001
    ....
  67. ncbi Platelet spherocytosis: a new bleeding disorder
    James G White
    Departments of Laboratory Medicine and Pathology, University of Minnesota Medical School, 420 Delaware Street SE, Mayo Mail Code 490, Minneapolis, MN 55455, USA
    Am J Hematol 70:158-66. 2002
    ..Differential interference phase contrast microscopy (DIC) revealed that his platelets were spherical in form...
  68. pmc Functional analysis of the leukemia protein ELL: evidence for a role in the regulation of cell growth and survival
    R W Johnstone
    The Peter MacCallum Cancer Institute, Gene Regulation Laboratory, Cancer Immunology Division, East Melbourne, 3002 Victoria, Australia
    Mol Cell Biol 21:1672-81. 2001
    ..These novel results indicate that ELL can regulate cell growth and survival and may explain how ELL translocations result in the development of human malignancies...
  69. ncbi Rickettsia rickettsii infection causes apoptotic death of cultured cerebellar granule neurons
    Suresh G Joshi
    J Med Microbiol 56:138-41. 2007
  70. ncbi C2-ceramide as a cell death inducer in HC11 mouse mammary epithelial cells
    Sung Hak Kim
    Department of Animal Science and Technology, School of Agricultural Biotechnology, Seoul National University, Suweon 441 744, South Korea
    Cancer Lett 203:191-7. 2004
    ....
  71. pmc Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells
    Guang Zhen Jin
    Division of Applied Life Science, Gyeongsang National University, Jinju 660 701, Korea
    J Vet Sci 8:377-82. 2007
    ..Thus, the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neurodegenerative disease process...
  72. ncbi In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R
    Ronald E Unger
    Institute of Pathology, Johannes Gutenberg University, Langenbeckstrasse 1, D 55101 Mainz, Germany
    Microvasc Res 64:384-97. 2002
    ..Thus, HPMEC-ST1.6R will be a valuable in vitro model system in which to study pathomechanisms and angiogenesis of the mature microvascular endothelium in vitro...
  73. ncbi Neural cell differentiation from retinal pigment epithelial cells of the newt: an organ culture model for the urodele retinal regeneration
    Yoko Ikegami
    Developmental Neurobiology Laboratory, Department of Biological Sciences, Faculty of Science, Nara Women s University, Nara 630 8506, Japan
    J Neurobiol 50:209-20. 2002
    ....
  74. ncbi Hepatic differentiation of mouse ES cells into BE cells in vitro
    An Bin Hu
    Organ Transplantation Center, the First Affiliated Hospital of Sun Yat Sen University, Guangzhou 510080, China
    Cell Biol Int 30:459-65. 2006
    ..4% on day 21. ES cells were found to be able to differentiate into BE cells when cultured using medium with appropriate cell growth factors. These BE cells may be a novel source of differentiated cell types for liver engineering...
  75. ncbi Protein kinase C-mediated cell death mode switch induced by high glucose
    R Fujita
    Division of Molecular Pharmacology and Neuroscience, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
    Cell Death Differ 10:1336-47. 2003
    ..All these results suggest that high-glucose treatment causes neuronal cell death mode switch by inhibiting necrosis, while inducing apoptosis, which is prevented by neurotrophic factors...
  76. ncbi Upregulation of heat shock protein expression by proteasome inhibition: an antiapoptotic mechanism in the lens
    Niranjan Awasthi
    Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, Newark, 07101, USA
    Invest Ophthalmol Vis Sci 46:2082-91. 2005
    ..The present study was conducted to test the hypothesis that proteasome inhibition can protect lens cells against apoptosis by upregulating heat shock protein (HSP) expression...
  77. ncbi Ubiquitin-proteasome-mediated degradation, intracellular localization, and protein synthesis of MyoD and Id1 during muscle differentiation
    Liping Sun
    Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26448-56. 2005
    ....
  78. ncbi The mammalian target of rapamycin regulates C2C12 myogenesis via a kinase-independent mechanism
    E Erbay
    Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
    J Biol Chem 276:36079-82. 2001
    ..Taken together, our observations suggest a novel mTOR signaling mechanism essential for skeletal muscle differentiation...
  79. ncbi Primary culture of medaka (Oryzias latipes) testis: a test system for the analysis of cell proliferation and differentiation
    Miyeoun Song
    Institute of Zoology, TU Dresden, Mommsenstrasse 13, 01062, Dresden, Germany
    Cell Tissue Res 313:107-15. 2003
    ..The test system is suitable for the study of the effect of substances which interfere with spermatogenesis in the vertebrate medaka model...
  80. ncbi Dictyostelium discoideum talin A is crucial for myosin II-independent and adhesion-dependent cytokinesis
    Makoto Hibi
    Graduate School of Science, Hokkaido University, Sapporo, Hokkaido 060 0810, Japan
    J Muscle Res Cell Motil 25:127-40. 2004
    ....
  81. ncbi Centrosome-dependent exit of cytokinesis in animal cells
    M Piel
    Institut Curie, Section Recherche, UMR 144 du CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France
    Science 291:1550-3. 2001
    ....
  82. ncbi Engineering cell de-adhesion dynamics on thermoresponsive poly(N-isopropylacrylamide)
    Beiyi Chen
    Center of Biotechnology, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 639798, Singapore
    Acta Biomater 4:218-29. 2008
    ..Furthermore, immunostaining of actin provides supporting evidence that the de-adhesion kinetics is correlated with the cytoskeleton transformation during cell de-adhesion below the lower solution critical temperature (LCST)...
  83. doi A meta-analysis of asbestos-related cancer risk that addresses fiber size and mineral type
    D Wayne Berman
    Aeolus, Inc, Albany, California 94706 1026, USA
    Crit Rev Toxicol 38:49-73. 2008
    ..If similar data become available for Quebec, perhaps these two size distributions can be used to eliminate the discrepancy between these two studies...
  84. ncbi Carbon source-dependent regulation of cell growth by murine protein kinase C epsilon expression in Saccharomyces cerevisiae
    A M Parissenti
    Department of Research, Northeastern Ontario Regional Cancer Centre, Sudbury, Canada
    J Cell Physiol 178:216-26. 1999
    ..Visualization of the cells by phase contrast microscopy indicated that murine PKCepsilon expression in the presence of glycerol resulted in a significant ..
  85. ncbi Retained functional integrity of bull spermatozoa after double freezing and thawing using PureSperm density gradient centrifugation
    W M C Maxwell
    Faculty of Veterinary Science, University of Sydney, Sydney, Australia
    Reprod Domest Anim 42:489-94. 2007
    ..spermatozoa was significantly improved by selection through the PureSperm gradient, whether measured by phase contrast microscopy (78.9 +/- 9.7 vs 90.4 +/- 4.0% NAR, p < 0.05) or flow cytometry (53.4 +/- 11.7 vs 76.3 +/- 6...
  86. ncbi Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells
    Josef Neumüller
    Center for Anatomy and Cell Biology, Department of Cell Biology and Ultrastructure Research, Medical University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria
    Histochem Cell Biol 126:649-64. 2006
    ..We characterized both cell populations by flow cytometry, phase contrast microscopy, fluorescence microscopy and confocal laser scanning microscopy as well as ultrastructurally using ..
  87. ncbi Effects of 5-fluouracil combined with sulfasalazine on human pancreatic carcinoma cell line BxPC-3 proliferation and apoptosis in vitro
    Zhe Huang
    Department of General Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China
    Hepatobiliary Pancreat Dis Int 6:312-20. 2007
    ....
  88. ncbi Protein kinase C modulator effects on parathyroid hormone-induced intracellular calcium and morphologic changes in UMR 106-H5 osteoblastic cells
    M Babich
    University of Illinois College of Medicine, Rockford, Illinois 61107 1897, USA
    J Cell Biochem 65:276-85. 1997
    ..The data suggest PKC contributes to UMR 106-H5 cell morphology and selectively regulates signal pathways activated by PTH to promote either cell contraction (cAMP) or extension (1,4,5-IP3/Cai2+)...
  89. ncbi Differentiated cellular function in fetal chondrocytes cultured with insulin-like growth factor-I and transforming growth factor-beta
    A J Nixon
    Comparative Orthopaedics Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    J Orthop Res 16:531-41. 1998
    ....
  90. doi Exposure to chrysotile asbestos associated with unpacking and repacking boxes of automobile brake pads and shoes
    A K Madl
    ChemRisk, Inc, 25 Jessie Street, Suite 1800, San Francisco, CA 94105, USA
    Ann Occup Hyg 52:463-79. 2008
    ..collected during the unpacking and repacking of boxes of asbestos-containing brakes were analyzed by phase contrast microscopy and transmission electron microscopy...
  91. doi A study of airborne chrysotile concentrations associated with handling, unpacking, and repacking boxes of automobile clutch discs
    George C T Jiang
    ChemRisk, Inc, 25 Jessie Street, Suite 1800, San Francisco, CA 94105, USA
    Regul Toxicol Pharmacol 51:87-97. 2008
    ..boxes of asbestos-containing clutches, and the subsequent cleanup and clothes handling, were analyzed by phase contrast microscopy (PCM) and transmission electron microscopy (TEM)...
  92. pmc Inhibition of plasminogen activators attenuates the death of differentiated retinal ganglion cells and stabilizes their neurite network in vitro
    Ronée Harvey
    Eye Research Institute of Oakland University, Rochester, MI 48309, USA
    Invest Ophthalmol Vis Sci 48:1884-91. 2007
    ....
  93. pmc Imatinib induces apoptosis by inhibiting PDGF- but not insulin-induced PI 3-kinase/Akt survival signaling in RGC-5 retinal ganglion cells
    Swarajit K Biswas
    Department of Medicine, Penn State Hershey Heart and Vascular Institute, Penn State College of Medicine, Hershey, PA 17033, USA
    Mol Vis 15:1599-610. 2009
    ..In the present study, we tested the hypothesis that the pro-survival effect of insulin-induced PI 3-kinase/Akt signaling is compromised by imatinib inhibition of PDGF receptor signaling in RGCs...
  94. ncbi Fibroblast growth factor 2 regulation of mitral valve interstitial cell repair in vitro
    Avrum I Gotlieb
    Vascular Research Laboratory, Toronto General Hospital, University Health Network, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    J Thorac Cardiovasc Surg 124:591-7. 2002
    ..We characterized the cellular morphology and pattern of repair of these interstitial cells in wounds produced in vitro and tested the hypothesis that fibroblast growth factor 2 enhances interstitial cell repair...
  95. ncbi In vitro induction of the acrosome reaction in ovine spermatozoa by calcium ionophore A23187
    O Ucar
    Department of Clinical Veterinary Sciences, Bristol University, Bristol, United Kingdom
    Acta Vet Hung 51:103-9. 2003
    ..After fixing in 10% formaldehyde, the number of spermatozoa that had undergone AR was determined by phase contrast microscopy. In pre-incubation samples, 21.3 +/- 3.3% of spermatozoa had undergone AR...
  96. ncbi Effect of an essential oil-containing dentifrice on dental plaque microbial composition
    C H Charles
    The Warner Lambert Consumer Healthcare Division of the Warner Lambert Consumer Group of Pfizer, Morris Plains, NJ 07950, USA
    Am J Dent 13:26C-30C. 2000
    ....
  97. pmc Development and application of different methods for the detection of Toxoplasma gondii in water
    Christina Kourenti
    Institute for Medical Parasitology, Medical Faculty, University of Bonn, 53127 Bonn, Germany
    Appl Environ Microbiol 69:102-6. 2003
    ..This is the first step in developing a systematic approach for the detection of Toxoplasma oocysts in water...
  98. ncbi An in vitro study on neuroprotective effects of serum containing Gengnianchun decoction and its main monomers against amyloid beta protein-induced cellular toxicity
    Wen Jun Wang
    Department of Integrated Traditional Chinese and Western Medicine, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China
    Zhong Xi Yi Jie He Xue Bao 8:67-73. 2010
    ....
  99. doi Airborne asbestos concentrations associated with heavy equipment brake removal
    A K Madl
    ChemRisk, LLC, San Francisco, CA 94105, USA
    Ann Occup Hyg 53:839-57. 2009
    ..n = 22), and ambient (n = 12) locations were collected during 12 brake changes and analyzed using phase contrast microscopy (PCM) [National Institute for Occupational Safety and Health (NIOSH) 7400] and TEM (NIOSH 7402)...
  100. ncbi Effects of autoclave sterilization on properties of dental rubber dam as related to its use as barrier membrane in guided tissue regeneration
    Wandee Apinhasmit
    Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
    J Periodontal Res 38:538-42. 2003
    ..The aim of the present study was to investigate the effects of autoclave sterilization on properties of RD as related to its use as a barrier membrane in guided tissue regeneration...
  101. ncbi Changes in elemental content during apoptotic cell death studied by electron probe X-ray microanalysis
    E Fernandez-Segura
    Department of Cell Biology, School of Medicine, University of Granada, Spain
    Exp Cell Res 253:454-62. 1999
    ..In addition, we demonstrated that these changes in elemental composition were not associated with loss of cell membrane integrity...

Research Grants96

  1. LISTERIA USES HOST CELL ACTIN TO SPREAD CELL TO CELL
    Frederick Southwick; Fiscal Year: 1993
    ..Other genes important for actin assembly will be sought by screening Tn9l6 Listeria mutants using phase contrast microscopy of infected PtK2 cells...
  2. GENETIC DETERMINANTS OF VIRULENCE IN VIBRIO CHOLERAE
    Ronald Taylor; Fiscal Year: 2007
    ....
  3. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2007
    ..The technical realization of PTI is relatively simple, requiring the combination of conventional optical phase-contrast microscopy and the already routinely used nanosecond laser. ..
  4. LIPID METABOLISM IN FAT CELLS
    Wen Guo; Fiscal Year: 2005
    ..abstract_text> ..
  5. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 2006
    ..3: To determine if eFGL binding to specific sperm ligands promotes sperm death and/or death cocoon polymerization.4: To define the function of eFGL by targeted disruption of the fgl2 gene in mice. ..
  6. PHARMACOLOGIC PREVENTION OF NEURONAL DEATH AFTER INJURY
    Keith Rich; Fiscal Year: 1993
    ....
  7. VISUAL NEURONS: DEVELOPMENT AND REGENERATION IN VITRO
    Ruben Adler; Fiscal Year: 1990
    ..A variety of analytical techniques will be used, including phase contrast microscopy, time-lapse video recording, scanning and transmission electron microscopy, immunocytochemistry, ..
  8. Functional Analysis of Mitotic Checkpoint Gene Bub1b
    Rakesh Goorha; Fiscal Year: 2005
    ..These studies are expected to establish the functions of Bub1b during mitosis and cytokinesis. ..
  9. CYTOMIMETIC ORGANIC CHEMISTRY
    FREDRIC MENGER; Fiscal Year: 2007
    ....
  10. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 1992
    ..Completion of these specific aims will provide new data on the mechanisms underlying the mosaic construction of the sperm plasma membrane and provide needed data on the molecular basis of specific fertilization events...
  11. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 1993
    ....
  12. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 2001
    ....
  13. Phase-sensitive x-ray breast tomosynthesis
    Xizeng Wu; Fiscal Year: 2010
    ..The project seeks to develop a prototype of the phase sensitive breast tomosynthesis for greatly enhancing the sensitivity and specificity of breast cancer detection and achieving many-fold reduction of radiation doses to breast. ..
  14. New probes for optical switching of protein interactions and protein fluorescence
    Gerard Marriott; Fiscal Year: 2007
    ....
  15. Rho GTPases in the lens- Role in Growth and Development
    P Rao; Fiscal Year: 2009
    ..abstract_text> ..
  16. Rho GTPases in the lens- Role in Growth and Development
    P Vasantha Rao; Fiscal Year: 2010
    ..abstract_text> ..
  17. Gene therapy in the cornea
    RAJIV MOHAN; Fiscal Year: 2009
    ..Rabbit models will be used to test Specific Aims 2 and 3 by delivering decorin into keratocytes with optimal selective gene transfer methods. ..
  18. Trabecular Meshwork Cytoskeletal Signaling-Regulation of Aqueous Humor Outflow
    P Rao; Fiscal Year: 2009
    ....
  19. RHO GTPASE IN THE LENS--ROLE IN GROWTH AND DEVELOPMENT
    P Rao; Fiscal Year: 2002
    ..abstract_text> ..
  20. Local Tomography for Radiation Therapy Tumor Imaging
    MARK ANASTASIO; Fiscal Year: 2005
    ..By increasing the conformality of the radiation therapy treatment procedure, the chances of survival for cancer patients could be improved while simultaneously minimizing the risk of radiation-induced complications. ..
  21. Role of Rho Kinase in Aqueous Humor Outflow Pathway
    P Rao; Fiscal Year: 2004
    ..Identification of specific signaling mechanisms regulating function of outflow pathway tissues could enable the design of target-specific approaches to the treatment of glaucoma. ..
  22. Gene therapy in the cornea
    RAJIV MOHAN; Fiscal Year: 2007
    ..Rabbit models will be used to test Specific Aims 2 and 3 by delivering decorin into keratocytes with optimal selective gene transfer methods. ..
  23. Role of Selenoprotein P in Male Fertility
    Gary Olson; Fiscal Year: 2007
    ..Aim 3: To identify and localize Se-P-binding proteins in the testis and epididymis. Aim 4: To determine the molecular basis for sperm defects in Se-P null mice. ..
  24. GENETIC DETERMINANTS OF VIRULENCE IN VIBRIO CHOLEREAE
    Ronald Taylor; Fiscal Year: 2001
    ..This pilus is immunogenic, yet dispensable for V. cholerae colonization, thus making it a candidate for pilin replacement by other type 4 pilins or partial substitution with heterologous epitopes. ..
  25. Technology Development in Optical Coherence Tomography
    Emily McDowell; Fiscal Year: 2006
    ..Additionally, I would like to investigate the prospect of combining the two techniques to allow for the creation of a novel endoscopic method for the detection of early stage cancers or other life-threatening pathologies. ..
  26. Hierachy Within Environmental Regulons
    Ronald Taylor; Fiscal Year: 2005
    ..A further understanding of virulence gene expression will also help in the development of ways to modulate it either in vivo or in vitro for improved vaccine production or overproduction of virulence factors for structural analyses. ..