Genomes and Genes
Summary: Techniques where DNA is delivered directly into organelles at high speed using projectiles coated with nucleic acid, shot from a helium-powered gun (gene gun). One of these techniques involves immunization by DNA VACCINES, which delivers DNA-coated gold beads to the epidermis.
Publications157 found, 100 shown here
- DNA immunization via intramuscular and intradermal routes using a gene gun provides different magnitudes and durations on immune responseKoichi Ito
Department of Immunology, Japan Science and Technology Corporation JST, Kitasato University School of Medicine, 1 15 1 Kitasato, Sagamihara, Kanagawa 228 8555, Japan
Mol Immunol 39:847-54. 2003....
- Cationic gold microparticles for biolistic delivery of nucleic acidsSergei Svarovsky
Center for Innovations in Medicine, The Biodesign Institute at Arizona State University, Tempe, AZ 85287, USA
Biotechniques 45:535-40. 2008..Along with its superior properties as a DNA delivery vehicle, charge-modified gold offers a unique opportunity to deliver various DNA formulations in addition to traditional naked DNA...
- Biolistic transfection of neuronal cultures using a hand-held gene gunSarah C R Lummis
Neurobiology Division, Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Nat Protoc 1:977-81. 2006..The method can be readily adapted to other cell types and tissues. The protocol can be completed in 1-2 h...
- A helium burst biolistic device adapted to penetrate fragile insect tissuesJ L Thomas
Unité Nationale Séricicole, INRA, 25 quai J J Rousseau, 69350 La Mulatière, France
J Insect Sci 1:9. 2001..No significant differences were noted between these two modes of delivery. The major improvement is the possibility of high pressure shooting correlated with appreciable penetration and a weak blast effect...
- Immunization by particle-mediated transfer of the granulocyte-macrophage colony-stimulating factor gene into autologous tumor cells in melanoma or sarcoma patients: report of a phase I/IB studyD M Mahvi
Department of Surgery, University of Wisconsin School of Medicine, Madison, WI 53792, USA
Hum Gene Ther 13:1711-21. 2002..This technique of gene transfer was safe and feasible, but resulted in clinically relevant levels of gene expression in only a minority of patients...
- The effects of gene gun delivered pIL-3 adjuvant on skin pathology and cytokine expressionK Matthews
Centre of Infectious Diseases, Royal Dick School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK
Vet Immunol Immunopathol 119:233-42. 2007..pIL-3 did not affect the expression of the immune response polarizing cytokines, IL-10 and IL-12...
- Nonviral approaches for targeted delivery of plasmid DNA and oligonucleotideShigeru Kawakami
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Pharm Sci 97:726-45. 2008..In this review, we will focus on the in vivo targeted delivery of pDNA and ODN using nonviral carriers...
- Transfection of liver in vivo by biolistic particle delivery: its use in the investigation of cytochrome P450 gene regulationRoongsiri Muangmoonchai
Department of Biochemistry and Molecular Biology, University College London, UK
Mol Biotechnol 20:145-51. 2002..However, when attached to the phenobarbital response element both promoters give the same fold-induction of reporter activity in response to phenobarbital...
- Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancerChi Chen Lin
Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
J Gene Med 10:679-89. 2008..To test whether this gun could inoculate a DNA vaccine that stimulates an anti-tumor Th1 immune response, we examined the effect of direct delivery of naked DNA (i.e. without any carrier) on the anti-tumor immune response of mice...
- Transfection by particle bombardment: delivery of plasmid DNA into mammalian cells using gene gunMasaki Uchida
The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N1AX, UK
Biochim Biophys Acta 1790:754-64. 2009..Recently, particle bombardment has become increasingly popular as a transfection method, because of a reduced dependency on target cell characteristics. In this study, we evaluated in vitro gene transfer by particle bombardment...
- Nonviral gene gun mediated transfer into the beating heartYukihiro Matsuno
First Department of Surgery, Gifu University School of Medicine, Gifu, Japan
ASAIO J 49:641-4. 2003..This technique is effective for gene transfer into the heart and may be one of the most useful methods for gene therapy for many cardiovascular diseases in the future...
- Transient expression and insertional mutagenesis of Puccinia triticina using biolisticsCraig A Webb
USDA-ARS, Plant Science and Entomology Research Unit, Kansas State University, Manhattan, KS 66506-5502, USA
Funct Integr Genomics 6:250-60. 2006..Using a linearized cloning plasmid, stable integration into the genome was achieved as demonstrated by PCR and sequencing analysis...
- Cross-reactive immune responses in mice after genetic vaccination with cDNA encoding hantavirus nucleocapsid proteinsMarie Lindkvist
Department of Medical Countermeasures, Division of NBC Defence, Swedish Defence Research Agency, SE 901 82 Umea, Sweden
Vaccine 25:1690-9. 2007..Our experimental data in combination with B-cell prediction software indicate that strong homologous virus species specific and cross-reactive epitopes are located around amino acid residue 40 in the nucleocapsid proteins...
- Dynamics modelling of biolistic gene gunsMingjun Zhang
Life Science and Chemical Analysis Division, Agilent Technologies, Santa Clara, CA 95051, USA
Phys Med Biol 52:1485-93. 2007..Simulations have also been conducted. The results agree well with experimental results in the open literature. The contribution of this paper includes a dynamic model for improving and manipulating performance of the biolistic gene gun...
- Organization of spines on the dendrites of Purkinje cellsJOHN O'BRIEN
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
Proc Natl Acad Sci U S A 103:1575-80. 2006..The purpose of this organization may therefore be to maximize the opportunity of different spines to interact with different axons...
- An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasmMiles R Armstrong
Plant Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, UK
Proc Natl Acad Sci U S A 102:7766-71. 2005..We suggest that Avr3a has undergone gene duplication and that an allele evading recognition by R3a arose under positive selection...
- Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissuesJean Luc Thomas
UNS INRA, 25 quai J J Rousseau, 69350 La Mulatière, France
J Insect Sci 3:17. 2003There are few powerful techniques available to transfect insect tissues. We previously used biolistics to transfect Bombyx mori embryos, and larval and pupal tissues (Thomas J-L et al. 2001...
- Transfection of rat or mouse neurons by biolistics or electroporationSulayman D Dib-Hajj
Department of Neurology, School of Medicine, Yale University, New Haven, CT, USA
Nat Protoc 4:1118-26. 2009..Although we have used sodium channels for the examples that we show here, these methods can also be used to study other types of molecules...
- Biolistics transformation of wheatCaroline A Sparks
Centre for Crop Genetic Improvement, Department of Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, UK
Methods Mol Biol 478:71-92. 2009....
- Removal of the circumsporozoite protein (CSP) glycosylphosphatidylinositol signal sequence from a CSP DNA vaccine enhances induction of CSP-specific Th2 type immune responses and improvesprotection against malaria infectionS Scheiblhofer
Immunology Group, Institute of Chemistry and Biochemistry, University of Salzburg, Salzburg, Austria
Eur J Immunol 31:692-8. 2001..Engineering plasmid constructs for proper cellular localization of gene products is a primary consideration for the preparation of optimally efficacious DNA vaccines...
- Efficient transformation of Dictyostelium discoideum with a particle inflow gunB Wetterauer
Zoologisches Institut der Universität München, Munich, Germany
Biochim Biophys Acta 1499:139-143. 2000..This is five times more than we achieve with the same vector using electroporation protocols. We also show that the particle inflow gun can be used for analysis of developmentally regulated gene expression in a transient assay...
- The delivery of PEBBLE nanosensors to measure the intracellular environmentA Webster
School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK
Biochem Soc Trans 35:538-43. 2007..This paper will overview several of the most popular methods used for the introduction of PEBBLE nanosensors to the cellular environment and discuss the efficacy of the techniques...
- Immunologic response to xenogeneic gp100 DNA in melanoma patients: comparison of particle-mediated epidermal delivery with intramuscular injectionBrian A Ginsberg
Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
Clin Cancer Res 16:4057-65. 2010..To further define the optimal vaccination strategy, we conducted a pilot clinical trial comparing i.m. injection with particle-mediated epidermal delivery (PMED)...
- PCR-generated linear DNA fragments utilized as a hantavirus DNA vaccineP Johansson
Department of Medical Countermeasures, Division of NBC Defence, Swedish Defence Research Agency, SE-90182 Ume, Sweden
Vaccine 20:3379-88. 2002....
- Microtargeted gene silencing and ectopic expression in live embryos using biolistic delivery with a pneumatic capillary gunOrit Shefi
Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, USA
J Neurosci 26:6119-23. 2006
- Biolistic delivery of Ca2+ dyes into plant and algal cellsJohn H F Bothwell
Marine Biological Association of the UK, The Laboratory, Citadel Hill, Plymouth PL1 2PB, UK
Plant J 46:327-35. 2006..Biolistic loading of differentiated plant cells is easier, quicker, and more widely applicable than micro-injection, and should broaden the study of plant signal transduction...
- Imaging calcium dynamics in the nervous system by means of ballistic delivery of indicatorsPetronella Kettunen
Department of Neuroscience, Nobel Institute for Neurophysiology, Karolinska Institutet, S-171 77 Stockholm, Sweden
J Neurosci Methods 119:37-43. 2002..The ballistic delivery of calcium indicators thus opens up many new avenues for further exploration of the structure and function of the nervous system from single spines to neuronal networks...
- Nonviral vectors in the new millennium: delivery barriers in gene transferM Nishikawa
Center for Pharmacogenetics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15213, USA
Hum Gene Ther 12:861-70. 2001..Successful clinical applications of nonviral vectors will rely on a better understanding of barriers in gene transfer and development of vectors that can overcome these barriers...
- Activation of systemic acquired silencing by localised introduction of DNAJ C Palauqui
Laboratoire de Biologie Cellulaire, INRA Centre de Versailles F 78026, Versailles, Cedex, France
Curr Biol 9:59-66. 1999..Graft-transmitted silencing is maintained in class II but not in class I plants when removed from silenced stocks, indicating similar requirements for spontaneous triggering and maintenance...
- The biolistic method as a tool for testing the differential activity of putative silkmoth chorion gene promotersL Kravariti
Department of Biochemistry and Molecular Biology, University of Athens, Panepistimioupolis, 15701, Athens, Greece
Insect Biochem Mol Biol 31:473-9. 2001..functional study of the tissue and temporal specificity of the 6F6 putative promoter region, we decided to apply biolistics. In the present work, use of a region from the 6F6...
- Direct transfection and activation of human cutaneous dendritic cellsA T Larregina
Department of Dermatology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA
Gene Ther 8:608-17. 2001..Taken together, our results demonstrate direct transfection, activation, and antigen-specific stimulatory function of in situ transduced human Langerhans cells...
- Targeting the vaccinia virus L1 protein to the cell surface enhances production of neutralizing antibodiesJoseph W Golden
Department of Molecular Virology, Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, United States
Vaccine 26:3507-15. 2008..Furthermore, our results demonstrate that a DNA-based vaccine is capable of establishing protection from lethal orthopoxvirus challenges when administered as a prime and single boost without requiring adjuvant...
- Prevention of long-term IgE antibody production by gene gun-mediated DNA vaccinationIsis Ludwig-Portugall
Clinical Research Unit of Allergology, Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany
J Allergy Clin Immunol 114:951-7. 2004..In addition, substantial numbers of IFN-gamma-producing CD8 + effector T cells were found in vaccinated mice. CONCLUSION: Gene gun-mediated DNA vaccination prevents the induction of long-lasting IgE antibody production...
- CD8+ T-cell-dependent immunity following xenogeneic DNA immunization against CD20 in a tumor challenge model of B-cell lymphomaMaria Lia Palomba
Department of Medicine and Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Clin Cancer Res 11:370-9. 2005..These results show that active immunization with xenogeneic DNA vaccines can induce CD8+ T cell-dependent immunity against CD20...
- Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virusNing Sun Yang
Institute of BioAgricultural Sciences, Academia Sinica, Taipei, 11529, Taiwan, ROC
J Gene Med 7:708-17. 2005..Foot-and-mouth disease virus (FMDV) causes a severe livestock disease, and the virus is an interesting target for virology and vaccine studies...
- Enhancement of immunogenicity of HPV16 E7 oncogene by fusion with E. coli beta-glucuronidaseMichal Smahel
Institute of Hematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic
J Gene Med 6:1092-101. 2004..In previous work, we prepared the E7GGG gene containing point mutations resulting in substitution of three amino acids in the pRb-binding site of the HPV16 E7 protein...
- Bystander help within a polyepitope DNA vaccine improves immune responses to influenza antigensM Baird
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand
Scand J Immunol 60:363-71. 2004..We conclude that a 'naked DNA' polyepitope vaccine generates specific responses to constituent epitopes and that adjacent irrelevant epitopes may enhance these responses...
- Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosaSukumar Saha
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Vaccine 24:6240-9. 2006..These results implied the possible clinical application of multivalent DNA vaccine against P. aeruginosa...
- DNA vaccination of pigs with open reading frame 1-7 of PRRS virusAnnette Malene Barfoed
Danish Institute for Food and Veterinary Research, Lindholm, DK 4771 Kalvehave, Denmark
Vaccine 22:3628-41. 2004..Pigs vaccinated with ORF1 or ORF4 were primed for antibody response against NSP2 or GP4, respectively. Neutralising antibodies were detected in all pigs, with ORF5 vaccinated pigs showing the highest titres...
- Protection of mice against challenge with Bacillus anthracis STI spores after DNA vaccinationUlrike K Hahn
Institut für Umwrelt und Tierhygiene, University of Hohenheim, Garbenstr 30, Stuttgart 70599, Germany
Int J Med Microbiol 294:35-44. 2004..Vaccination with either pSecTag PA83 or pCMV/ER PA83 showed significant protection of A/J mice against infection with B. anthracis STI spores...
- [Construction of DNA vaccine of Trichinella spiralis muscle larvae and its expression in mice]Jing Cui
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22:5-8. 2004..RESULTS AND CONCLUSION: The recombinant plasmid pcDNA3-TspE1 was successfully constructed and expressed in the BALB/c mice...
- Ubiquitin-fusion degradation pathway plays an indispensable role in naked DNA vaccination with a chimeric gene encoding a syngeneic cytotoxic T lymphocyte epitope of melanocyte and green fluorescent proteinManxin Zhang
Department of Microbiology and Immunology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Immunology 112:567-74. 2004..It is noteworthy to document that genetic immunization with pGFP plus pTRP-2(181-188) failed to exert the antitumour immunity...
- Particle-mediated DNA vaccine delivery to the skinJoel R Haynes
PowderJect Vaccines, Inc, 8551 Research Way, Middleton, WI 53562, USA
Expert Opin Biol Ther 4:889-900. 2004..Particle-mediated DNA vaccines represent a promising tool for developing candidate vaccines against some of the more difficult infectious, parasitic and oncologic disease targets...
- Imiquimod and resiquimod in a mouse model: adjuvants for DNA vaccination by particle-mediated immunotherapeutic deliveryLindy L Thomsen
LindyDepartment of Immunotherapeutics, GlaxoSmithKline Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK
Vaccine 22:1799-809. 2004..Collectively, these studies suggest that both imiquimod and resiquimod may be suitable adjuvants for therapeutic DNA vaccines requiring induction of potent cytotoxic T cell responses...
- Priming of piglets against enterotoxigenic E. coli F4 fimbriae by immunisation with FAEG DNAT Verfaillie
Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, University Ghent, Salisburylaan 133, Merelbeke B 9820, Belgium
Vaccine 22:1640-7. 2004..The low responses to the pcDNA1/faeG19 DNA vaccination suggest that delivery of the DNA and/or the expression of the faeG gene should be improved...
- Combined immunization with fusion genes of mutated E7 gene of human papillomavirus type 16 did not enhance antitumor effectDana Pokorna
Institute of Hematology and Blood Transfusion, Department of Experimental Virology, U Nemocnice 1, 128 20 Prague 2, Czech Republic
J Gene Med 7:696-707. 2005..Its immunogenicity has been substantially enhanced by fusion with several proteins of various origins and functions. Different mechanisms have been responsible for increased vaccination efficacy of fusion proteins...
- Effect of cytokine-encoding plasmid delivery on immune response to Japanese encephalitis virus DNA vaccine in miceKaushik Bharati
National Institute of Immunology, New Delhi, India
Microbiol Immunol 49:349-53. 2005..However, intra-dermal co-administration of pMEa and pGM-CSF in mice using the gene gun, enhanced anti-JEV antibody titers resulting in an increased level of protection in mice against lethal JEV challenge...
- Evaluation of a needle-free delivery platform for prime-boost immunization with DNA and modified vaccinia virus ankara vectors expressing herpes simplex virus 2 glycoprotein DClement A Meseda
Laboratory of DNA Viruses, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20852, USA
Viral Immunol 19:250-9. 2006..Thus, needle-free delivery of plasmid DNA may provide additional flexibility and options for effective prime-boost vaccination...
- Comparative evaluation of CD8+CTL responses following gene gun immunization targeting the skin with intracutaneous injection of antigen-transduced dendritic cellsEvelyn Gaffal
Laboratory of Experimental Dermatology, Department of Dermatology, University of Bonn, Sigmund Freud Street 25, D 53105 Bonn, Germany
Eur J Cell Biol 86:817-26. 2007..Further investigations will have to address the role of Langerhans cells for the activation of cellular immunity in the skin...
- Suppression of major histocompatibility complex class II-associated invariant chain enhances the potency of an HIV gp120 DNA vaccineXueqing Lu
Antigen Express, Inc, Worcester, MA 01606 2758, USA
Immunology 120:207-16. 2007..Activated CD4(+) T cells locally strengthen the response of CD8(+) CTL, thus enhancing the potency of a DNA vaccine...
- Essential sequence of influenza neuraminidase DNA to provide protection against lethal viral infectionXiangzhong Li
College of Life Sciences, Hunan Normal University, Changsha, Hunan, People's Republic of China
DNA Cell Biol 25:197-205. 2006..The nt58-1299 of NA DNA may play an important role in protection against influenza virus...
- Expression library immunization confers partial protection against Chlamydia muridarum genital infectionCelia L McNeilly
School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU 7XH, Surrey, UK
Vaccine 25:2643-55. 2007..These results demonstrate that the C. muridarum genome can be screened for individual vaccine candidates by genetic immunization and that the screen produces novel and partially protective vaccine candidates...
- Abeta42 gene vaccination reduces brain amyloid plaque burden in transgenic miceBaoxi Qu
Alzheimer's Diseases Center, Department of Neurology, University of Texas Southwestern Medical Center, 5323, Harry Hines, Dallas, 75390-9036, USA
J Neurol Sci 244:151-8. 2006..Abeta42 gene vaccination significantly reduces the Abeta42 burden of the brain in treated APPswe/PS1DeltaE9 transgenic mice with no overlap between treated and control mice...
- The involvement of immunoproteasomes in induction of MHC class I-restricted immunity targeting Toxoplasma SAG1Kazunari Ishii
Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Microbes Infect 8:1045-53. 2006....
- Analysis of humoral immunity of hepatitis D virus DNA vaccine generated in mice by using different dosage, gene gun immunization, and in vivo electroporationYih-Tsong Shiau
Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taiwan, ROC
J Chin Med Assoc 69:7-13. 2006..DNA vaccine with a L-HDAg construct may not be a candidate HDV vaccine to generate anti-HDV humoral immunity...
- Gene gun immunization with clinically relevant allergens aggravates allergen induced pathology and is contraindicated for allergen immunotherapySandra Scheiblhofer
Christian Doppler Laboratory for Allergy Diagnosis and Therapy, Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria
Mol Immunol 44:1879-87. 2007....
- DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)Dana Pokorna
Department of Experimental Virology, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic
Oncol Rep 14:1045-53. 2005..Their antitumor effect was comparable to those of the whole E7 gene and Sig/E7GGG/LAMP-1 fusion gene. There was no relevant difference between immune responses elicited by CP-E7 and CPdel-E7 DNA vaccination...
- Gene-gun biolistic immunization encoding glutamic acid decarboxylase: a model for studying Langerhans cell abnormalities and mimicry in the nonobese diabetic mouseBéatrice Joussemet
Immuno endocrinology Unit, ENVN INRA University, ENVN, Atlanpole La Chantrerie, BP 40706, 44307 Nantes Cedex 03, France
Ann N Y Acad Sci 1051:613-25. 2005..The gene-gun technique could thus provide an interesting model for studying skin DC abnormalities in NOD mice and their potential implication of presenting mimetic peptides that modulate the autoimmune response in T1D...
- CpG motif acts as a 'danger signal' and provides a T helper type 1-biased microenvironment for DNA vaccinationLing Liu
Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical College, Fudan University, Shanghai, China
Immunology 115:223-30. 2005..These results may help to explain the mechanism of different types of immune response induced by DNA vaccines delivered by different routes and facilitate the application of DNA vaccines...
- B lymphocytes participate in cross-presentation of antigen following gene gun vaccinationHuiming Hon
Department of Microbiology and Immunology, Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA
J Immunol 174:5233-42. 2005..Collectively, these data indicate that broad expression of the immunogen is required for optimal induction of protective acquired immunity...
- pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infectionAdriana Malheiro
Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av Café S N, 14040 903 Ribeirao Preto, SP, Brazil
Vaccine 26:305-15. 2008..canis infection, suggesting their possible use in further combined therapeutic interventions...
- In vivo electroporation of skeletal muscles increases the efficacy of Japanese encephalitis virus DNA vaccineChang-Jer Wu
Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan
Vaccine 22:1457-64. 2004..These results suggest that in vivo electroporation can be used as an efficient gene delivery system for DNA vaccines to provide efficient protection against viral infection...
- Influence of routes and administration parameters on antibody response of pigs following DNA vaccinationAnnette Malene Barfoed
Laboratory of Virology and Immunology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, , DK-1870 Frederiksberg C, Denmark
Vaccine 22:1395-405. 2004..m.)/intradermal (i.d.) injection of plasmid DNA. This indicates, that the ear is an attractive site for gene gun vaccination of pigs...
- Comparison of individual and combination DNA vaccines for B. anthracis, Ebola virus, Marburg virus and Venezuelan equine encephalitis virusJenny Riemenschneider
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA
Vaccine 21:4071-80. 2003....
- Comparison of the CD8+ T cell responses and antitumor effects generated by DNA vaccine administered through gene gun, biojector, and syringeCornelia Trimble
Department of Obstetrics and Gynecology, The Johns Hopkins Medical Institution, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Vaccine 21:4036-42. 2003..m.) and biojector administrations. Thus, our data suggest that DNA vaccination via gene gun represents the most potent regimen for DNA administration...
- [Antitumor effect of gene gun-mediated DNA vaccine pWRG-neu immunization in C57BL/6 mice]Yuan-yuan Zha
National Laboratory of Molecular Oncology, Cancer Institute (Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China
Zhonghua Zhong Liu Za Zhi 25:320-4. 2003..CONCLUSION: Gene gun-mediated gene transfer is effective and practicable. DNA vaccine pWRG-neu is potent in preventing subsequent tumor cells challenge, inhibiting the tumor growth and metastasis...
- Gene transfer methods for CNS organotypic cultures: a comparison of three nonviral methodsR C Murphy
Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002, USA
Mol Ther 3:113-21. 2001Organotypic slice cultures from postnatal day 12 mouse cerebellum were transfected using three nonviral methods: biolistics (gene gun), lipotransfection, and electroporation...
- DNA vaccines, combining form of antigen and method of delivery to raise a spectrum of IFN-gamma and IL-4-producing CD4+ and CD8+ T cellsAlp E Oran
Emory Vaccine Center and Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA
J Immunol 171:1999-2005. 2003..Studies in B cell knockout and hen egg lysozyme Ig transgenic mice revealed that B cells were required for the generation of IL-4-producing CD8(+) T cells...
- Lipopeptides: adjuvanticity in conventional and genetic immunizationK Mittenbühler
Institut für Molekulare Medizin und Zellforschung, Universitatsklinikum Freiburg, AK Tumorimmunologie Vakzine, Stefan Meier Str 8, D 79104 Freiburg, Germany
FEMS Immunol Med Microbiol 37:193-200. 2003..We monitored a lipopeptide-dependent shift from a Th1-type to Th2-type response, when DNA immunization was followed by i.p. administration of the lipopeptide adjuvant...
- The protective efficacy of MSP4/5 against lethal Plasmodium chabaudi adami challenge is dependent on the type of DNA vaccine vector and vaccination protocolA Rainczuk
Department of Biochemistry and Molecular Biology, The Cooperative Research Centre for Vaccine Technology, Clayton 3800, Australia
Vaccine 21:3030-42. 2003..The results suggest that the enhancement of survival against lethal blood-stage malaria challenge after utilizing MSP4/5 DNA vaccination is therefore highly dependent on the route and type of vaccine vector employed...
- Immunologic analysis induced by DNA vaccine encoding E protein of Beijing-1 strain derived from Japanese encephalitis virusGuo he Feng
Department of Infectious Diseases, Second Affiliated Hospital of China Medical University, Shenyang, China
Intervirology 50:93-8. 2007..This study aimed to construct a recombinant encoding E protein of the Beijing-1 strain derived from Japanese encephalitis virus and analyze the humoral, cellular and protective immunity induced by the above recombinant...
- Gene gun bombardment with gold particles displays a particular Th2-promoting signal that over-rules the Th1-inducing effect of immunostimulatory CpG motifs in DNA vaccinesRichard Weiss
Institute of Chemistry and Biochemistry, University of Salzburg, Hellbrunner Street 34, A-5020 Salzburg, Austria
Vaccine 20:3148-54. 2002..Furthermore, the data also indicate a strong and even systemic adjuvant effect of the gene gun shot itself...
- Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genesRabie M Mohamed
Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan
Vaccine 21:2852-61. 2003..gondii organisms compared with 50 microg of T.g.HSP70 gene vaccination by intramuscular (i.m.) or intraperitoneal (i.p.) injection. The vaccine effects of T.g.HSP70 gene persisted for more than 3 months...
- Simultaneous GeneGun immunisation with plasmids encoding antigen and GM-CSF: significant enhancement of murine antivenom IgG1 titresR A Harrison
Alistair Reid Venom Research Unit, Division of Molecular Biology and Immunology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
Vaccine 20:1702-6. 2002..This report illustrates that DNA immunisation protocols to achieve high-titre, venom toxin-specific antibody production are well advanced and encourage the development of a DNA-based approach to antivenom production...
- DNA vaccine against Friend erythroleukaemia virusP Sima
Institute of Haematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic
Folia Biol (Praha) 48:43-50. 2002..The FV type A or P was used as a challenge. The immunization with gag- and env-derived vaccines resulted in protective immunity in a high proportion of mice...
- Gene gun-based co-immunization of merozoite surface protein-1 cDNA with IL-12 expression plasmid confers protection against lethal Plasmodium yoelii in A/J miceTohru Sakai
Department of Parasitology and Immunology, University of Tokushima School of Medicine, Japan
Vaccine 21:1432-44. 2003..yoelii. The results indicate that combined vaccination with DNA encoding antigenic peptides plus IL-12 DNA provides a strategy for improving the prophylactic efficacy of a vaccine for malaria infection...
- Comparative efficacy of the Schistosoma mansoni nucleic acid vaccine, Sm23, following microseeding or gene gun deliveryAkram A Da'Dara
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
Parasite Immunol 24:179-87. 2002..However, the protective effect of the vaccine was not affected by coadministering plasmids encoding either IL-12 or IL-4 using the microseeding technique...
- Drug-inducible, dendritic cell-based genetic immunizationLaura Timares
Center for Biomedical Inventions, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
J Immunol 170:5483-90. 2003..Our results indicate that direct transfection is a predominant pathway for LC Ag loading. The ability to regulate transgene expression with inducible DC-based vaccines demonstrates a new level of immunological control...
- Vaccination of rhesus macaques against dengue-2 virus with a plasmid DNA vaccine encoding the viral pre-membrane and envelope genesRobert Putnak
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
Am J Trop Med Hyg 68:469-76. 2003..Long-term protection following DNA vaccination may require revaccination, higher doses of DNA, or a vaccine that contains additional epitopes or adjuvants...
- Novel antigen identification method for discovery of protective malaria antigens by rapid testing of DNA vaccines encoding exons from the parasite genomeDiana Haddad
Naval Medical Research Center, Walter Reed Army Institute of Research, Silver Spring, MD, USA
Infect Immun 72:1594-602. 2004..We conclude that high-throughput cloning of exons into DNA vaccines and their screening is feasible and can rapidly identify new malaria vaccine candidate antigens...
- Induction of anti-hepatitis B virus immune responses through DNA immunizationMichael Geissler
Department of Internal Medicine II, University of Freiburg, Germany
Methods Mol Med 96:43-54. 2004
- Schistosoma mansoni miracidia transformed by particle bombardment infect Biomphalaria glabrata snails and develop into transgenic sporocystsOliver Heyers
Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin, 10115 Berlin, Germany
Exp Parasitol 105:174-8. 2003..Our findings indicated that it is feasible to return transgenic miracidia to the life cycle, a crucial step for the establishment of a transgenesis system for schistosomes...
- Protective DNA vaccination by particle bombardment using BAC DNA containing a replication-competent, packaging-defective genome of herpes simplex virus type IMark Suter
Institute of Virology, University of Zurich, Switzerland
Methods Mol Biol 256:303-8. 2004
- Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinomaArianna Smorlesi
Laboratory of Tumor Immunology, Immunology Center, INRCA Gerontology Research Department, Via Birarelli 8, 60121 Ancona, Italy
Vaccine 24:1766-75. 2006....
- Induction of murine ras oncogene peptide-specific T cell responses by immunization with plasmid DNA-based minigene vectorsPeter Lindinger
Immunology Group, Institute of Chemistry and Biochemistry, University of Salzburg, Salzburg, Austria
Vaccine 21:4285-96. 2003....
- Individual analysis of mice vaccinated against a weakly immunogenic self tumor-specific antigen reveals a correlation between CD8 T cell response and antitumor efficacyAntonio Rosato
Immunology Section, Department of Oncology and Surgical Sciences, University of Padua, Padua, Italy
J Immunol 171:5172-9. 2003..These results highlight the critical need to identify reliable, specific immunological parameters that may predict success or failure of an immune response against cancer...
- DNA immunization of the grafted liver by particle-mediated gene gunMasahiko Nakamura
Division of Organ Replacement Research and Molecular Immunology, Jichi Medical School, Tochigi, Japan
Transplantation 76:1369-75. 2003..CONCLUSIONS: A DNA vaccine to the liver may be one strategy for preventing infectious disease associated with liver transplantation under tacrolimus treatment...
- DNA immunization of mice with a plasmid encoding Neisseria gonorrhea PorB protein by intramuscular injection and epidermal particle bombardmentW Zhu
Department of Medicine, Division of Infectious Diseases, University of North Carolina at Chapel Hill, CB 7031, 8431 Medicine and Biomolecular Research Building, Chapel Hill, NC 27599, USA
Vaccine 22:660-9. 2004..Based on different prime-boost protocols, we could polarize immune responses to predominantly Th1 or Th2, which should enable future studies of the types of immune responses that are protective in mouse models of gonorrhea...
- A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlingsXinlu Chen
University of Tennessee, Department of Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN, 37996, USA
Biotechnol Biofuels 3:9. 2010..has previously been reported after cocultivation of explants with Agrobacterium and biolistics of embryogenic calli...
- [Transgenic maize plants with low copy number of foreign genes were produced with maize Ubi-1 promoter]Zi Qin Xu
Provincial Key Laboratory of Biotechnology, Northwest University, Xi an 710069, China
Sheng Wu Gong Cheng Xue Bao 20:120-5. 2004Direct DNA delivery procedures (include biolistics method) often resulted in multiple copies of the transgenes in transformants and certain copies of them were rearranged...
- Regulatory domains in the intergenic region of the oxytocin and vasopressin genes that control their hypothalamus-specific expression in vitroRaymond L Fields
Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
J Neurosci 23:7801-9. 2003..organotypic slice-explant cultures of rat hypothalamus as in vitro models, and particle-mediated gene transfer (biolistics) transfection methods to identify critical DNA sequences in the IGR between the OT and VP genes responsible for ..
- Biolistics for high-throughput transformation and RNA interference in Drosophila melanogasterJenna L Yuen
Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada
Fly (Austin) 2:247-54. 2008..In this study, we describe the use of biolistics as a higher-throughput method of nucleic acid delivery. By bombarding dechorionated D...
- Development of an edible rabies vaccine in maize using the Vnukovo strainE Loza-Rubio
National Center of Veterinary Microbiology CENID Microbiologia, INIFAP, Mexico
Dev Biol (Basel) 131:477-82. 2008..Maize embryogenic callus were transformed with the above construct by biolistics. Regenerated maize plants were recovered and grown in a greenhouse...
- Plant cell-directed control of virion sense gene expression in wheat dwarf virusP S Gooding
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK and Plant Breeding International, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK
Nucleic Acids Res 27:1709-18. 1999We have used particle bombardment (biolistics) to deliver replication-competent wheat dwarf virus (WDV)-based constructs, carrying reporter gene sequences fused to the virion sense promoter (Pv) or the CaMV 35S promoter, to suspension ..
- Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genesHuixia Wu
Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
Transgenic Res 17:425-36. 2008Genetic transformation of wheat, using biolistics or Agrobacterium, underpins a range of specific research methods for identifying genes and studying their function in planta...
- Long-term stability of marker gene expression in Prunus subhirtella: a model fruit tree speciesFatemeh Maghuly
Plant Biotechnology Unit, Institute of Applied Microbiology, Department of Biotechnology, BOKU, Nussdorfer Laende 11, A 1190 Vienna, Austria
J Biotechnol 127:310-21. 2007Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumno rosa (PAR) ..
- Transient expression of DNA and RNA in parasitic helminths by using particle bombardmentR E Davis
Department of Biological Sciences, Fordham University, Bronx, NY 10458, USA
Proc Natl Acad Sci U S A 96:8687-92. 1999..We evaluated particle bombardment (biolistics) as a strategy to introduce and express nucleic acids in these multicellular parasites...
- Molecular morphology of neuronal apoptosis: analysis of caspase 3 activation during postnatal development of mouse cerebellar cortexLaura Lossi
Department of Veterinary Morphophysiology, Rita Levi Montalcini Center for Brain Repair, Via Leonardo da Vinci 44, 10095 Grugliasco, Italy
J Mol Histol 35:621-9. 2004..situ labeling of apoptotic cells, and analysis of cerebellar slices following particle-mediated gene transfer (biolistics), we have studied the relationship of cell death and cleavage of caspase 3, a key molecule in the execution of ..
- Hormonal regulation of gluconeogenesis in cereal aleurone is strongly cultivar-dependent and gibberellin action involves SLENDER1 but not GAMYBPeter J Eastmond
Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, UK
Plant J 44:483-93. 2005..b>Biolistics using a promoter reporter showed that GA and ABA regulate ICL at the level of transcription...
- The complete nucleotide sequence of a new bipartite begomovirus from Brazil infecting AbutilonT Paprotka
Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universitat Stuttgart, Pfaffenwaldring 57, 70550, Stuttgart, Germany
Arch Virol 155:813-6. 2010..AbMBV is transmissible to Nicotiana benthamiana and Malva parviflora by biolistics of rolling-circle amplification products and induces characteristic mosaic and vein-clearing symptoms in M...
- A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombinationS Choi
Division of Biology 147 75, California Institute of Technology, Pasadena, CA 91125, USA
Nucleic Acids Res 28:E19. 2000With current plant transformation methods ( Agrobacterium, biolistics and protoplast fusion), insertion of DNA into the genome occurs randomly and in many instances at multiple sites...
Research Grants108 found, 100 shown here
- DNA Immunizations with GAD65 to Induce TolerancePENELOPE MOREL; Fiscal Year: 2002..abstract_text> ..
- Microglial Neuroprotection and NeurotoxicityGreer Murphy; Fiscal Year: 2007..of M-CSFR on microglia protects neurons from excitotoxic injury in slice cultures transfected using biolistics, and in a microglial-hippocampal organotypic co-culture system...
- High-Efficiency Biolistic Device for Brain TransfectionDONALD LO; Fiscal Year: 2006device, based on particle-mediated gene transfer (or 'biolistics'), for the identification and validation of candidate therapeutic drugs and targets...
- In Vivo Study of Synapses in Alzheimer's Disease ModelsWenbiao Gan; Fiscal Year: 2004....
- Therapeutic antibodies for lethal anthrax infectionJohn Herrmann; Fiscal Year: 2003..At the end of this phase 1 study, a standardized schedule will be established in phase II to prepare for large-scale production and testing of this much needed, perhaps essential, product in biodefense against anthrax attacks. ..
- Links among synaptic activity, translation and structurePeter Vanderklish; Fiscal Year: 2003....
- Naltrexone as a Novel Treatment for Diabetic KeratopathyIAN ZAGON; Fiscal Year: 2007..The proposed use of biotherapy with NTX is an innovative approach whereby the patient's own growth regulatory mechanisms are manipulated to correct complications from diabetes. ..
- Immunobiological Role of Fertilization Antigen(FA-1)RAJESH NAZ; Fiscal Year: 2007....
- Non Viral Gene Therapy for Lung InjurySong Li; Fiscal Year: 2010..Public Health Relevance: This study is aimed to develop a novel gene therapy for the treatment of pulmonary injury such as acute respiratory distress syndrome and pulmonary fibrosis. ..
- Molecular and Cellular Basis of PCB Developmental NeurotoxicityIsaac N Pessah; Fiscal Year: 2010....
- Immune molecules in early postnatal nervous system developmentA Kimberley McAllister; Fiscal Year: 2010....
- SYNAPSE FORMATION AND REFINEMENT IN THE VISUAL SYSTEMA Kimberley McAllister; Fiscal Year: 2010..Understanding the cellular and molecular mechanismsof synapse formation could revolutionize our ability to identify,prevent, and treat these developmental disorders. ..
- Circadian regulation of microRNA biogenesis and functionPeter W Vanderklish; Fiscal Year: 2010..Our studies will define a mechanistic link between circadian rhythm and protein synthesis in neurons, providing fundamental insights into the control of brain function and neurological diseases. ..
- Molecular and Cellular Basis of PCB Developmental NeurotoxicityPAMELA J contact LEIN; Fiscal Year: 2011....
- K12 Expression: Cornea-Type Epithelial DifferentiationWINSTON KAO; Fiscal Year: 2007..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
- Experience-Dependent Plasticity Of Synaptic StructureWenbiao Gan; Fiscal Year: 2007..Together, these studies will provide a comprehensive view of the role of experience in shaping synaptic connectivity at different stages of life and suggest potential strategies for functional rehabilitation after brain injury ..
- Mechanism of Eliciting Antitumor Cell Immunity Via Lentivector ImmunizationYukai He; Fiscal Year: 2007..The knowledge gained from these studies will assist the design of more effective and safer genetic immunization approaches for antitumor immunotherapy. ..
- SURGICAL ONCOLOGY RESEARCH TRAINING PROGRAMDavid Mahvi; Fiscal Year: 2007..Trainees will be provided with the background to organize, develop, and lead translational and clinical surgical oncology research programs in a variety of academic, governmental, or industrial institutions. ..
- Non Viral Gene Therapy for Lung InjurySong Li; Fiscal Year: 2011..Public Health Relevance: This study is aimed to develop a novel gene therapy for the treatment of pulmonary injury such as acute respiratory distress syndrome and pulmonary fibrosis. ..
- Structure/Function Relationship of The Lumican GeneWINSTON KAO; Fiscal Year: 2007..g., persistent and excessive inflammation, corneal transparency of injured cornea, improved wound healing of diabetes, etc. ..
- CD20 DNA VACCINATION FOR B-CELL LYMPHOMAMaria Palomba; Fiscal Year: 2007..Furthermore, she proposes to explore the effect of CD20 immunization in the post-bone marrow transplant settings. Finally, the candidate proposes to carry out a phase I clinical trial of CD20 DNA vaccine in patients with lymphoma. ..
- Structure/Function Relationship of The Lumican GeneWinston W Kao; Fiscal Year: 2010..g., persistent and excessive inflammation, corneal transparency of injured cornea, improved wound healing of diabetes, etc. ..
- Comparative and functional genomics of C. neoformansJames W Kronstad; Fiscal Year: 2010..The research will specifically examine the potential to control infections by targeting the ability of fungal pathogens to acquire the nutrient iron during infection. ..
- MATHEMATICAL MODELING OF IMMUNOLOGICAL REACTIONSPENELOPE MOREL; Fiscal Year: 2001..abstract_text> ..
- Roles of Growth Factors on Corneal MorphogenesisWinston W Kao; Fiscal Year: 2010..Data obtained will yield useful information for a better understanding of corneal diseases at molecular and cellular levels in vivo and serve as basis for designing treatment regiments for corneal wound healing. ..
- Influenza DNA Vaccine for Biodefense/Pandemic ReadinessJoel Haynes; Fiscal Year: 2004..These nozzles will be designed to facilitate manufacturing scale-up and to deliver increased DNA/gold payloads to larger skin target areas as a means of increasing the effective vaccine dose. ..
- Roles of Growth Factors on Corneal MorphogenesisWINSTON KAO; Fiscal Year: 2005..g., epithelial debridement, incision, alkali burn. ..
- Regulation of Antitumor Immunity by DC SubsetsYukai He; Fiscal Year: 2006..The institute is committed to fully support the candidate by providing access to all of the resources. Together, the candidate, research proposal, and the environment provide a strong application for this award. ..
- Comparative and functional genomics of C. neoformansJames Kronstad; Fiscal Year: 2007..We will compare the organization and transcriptional regulation of these genes (and a third iron-regulated gene CnURF1) between serotypes. We will also disrupt the genes to examine their role in virulence. ..
- Mice Overexpressing rtTA and Cre in Corneal EpitheliumWINSTON KAO; Fiscal Year: 2004..These mouse lines will be useful in preparing mouse lines for studies of altered genetic functions from the corneal epithelium-specific gene ablation and overexpression of reporter genes. ..
- Immune response to Pneumocystis in simian model of AIDSKaren Norris; Fiscal Year: 2007....
- Pharmacogenetic Therapy of Pulmonary HypertensionSong Li; Fiscal Year: 2006..g. nitric oxide and oxygen), provide an approach to prevent the progressive pathological aspects of PHT and ameliorate return towards more normal pulmonary vascular structure and function. ..