biolistics

Summary

Summary: Techniques where DNA is delivered directly into organelles at high speed using projectiles coated with nucleic acid, shot from a helium-powered gun (gene gun). One of these techniques involves immunization by DNA VACCINES, which delivers DNA-coated gold beads to the epidermis.

Top Publications

  1. ncbi DNA immunization via intramuscular and intradermal routes using a gene gun provides different magnitudes and durations on immune response
    Koichi Ito
    Department of Immunology, Japan Science and Technology Corporation JST, Kitasato University School of Medicine, 1 15 1 Kitasato, Sagamihara, Kanagawa 228 8555, Japan
    Mol Immunol 39:847-54. 2003
  2. ncbi Nonviral vectors in the new millennium: delivery barriers in gene transfer
    M Nishikawa
    Center for Pharmacogenetics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15213, USA
    Hum Gene Ther 12:861-70. 2001
  3. doi Helios Gene Gun-mediated transfection of the inner ear sensory epithelium
    Inna A Belyantseva
    Laboratory of Molecular Genetics, Section on Human Genetics, NIDCD, National Institutes of Health, Rockville MD, USA
    Methods Mol Biol 493:103-23. 2009
  4. doi High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants
    Elibio L Rech
    Embrapa Genetic Resources and Biotechnology, Laboratory of Gene Transfer, Biotechnology Unit, Parque Estação Biológica PqEB W5 Norte, Brasília 70770 900, DF, Brazil
    Nat Protoc 3:410-8. 2008
  5. ncbi The myth of plant transformation
    Abdul Qayyum Rao
    National Centre of Excellence in Molecular Biology, 87 West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan
    Biotechnol Adv 27:753-63. 2009
  6. ncbi Transfection by particle bombardment: delivery of plasmid DNA into mammalian cells using gene gun
    Masaki Uchida
    The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N1AX, UK
    Biochim Biophys Acta 1790:754-64. 2009
  7. ncbi Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles
    Paul A Raju
    The PowderJect Centre for Gene and Drug Delivery Research, University of Oxford, 43 Banbury Rd, Oxford OX2 6PE, UK
    Vaccine 24:4644-7. 2006
  8. ncbi DNA vaccine against Friend erythroleukaemia virus
    P Sima
    Institute of Haematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic
    Folia Biol (Praha) 48:43-50. 2002
  9. pmc An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm
    Miles R Armstrong
    Plant Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, UK
    Proc Natl Acad Sci U S A 102:7766-71. 2005
  10. ncbi Downregulation of IgE antibody and allergic responses in the lung by epidermal biolistic microparticle delivery
    Mark Kendall
    The PowderJect Centre for Gene and Drug Delivery Research, University of Oxford, UK
    J Allergy Clin Immunol 117:275-82. 2006

Detail Information

Publications176 found, 100 shown here

  1. ncbi DNA immunization via intramuscular and intradermal routes using a gene gun provides different magnitudes and durations on immune response
    Koichi Ito
    Department of Immunology, Japan Science and Technology Corporation JST, Kitasato University School of Medicine, 1 15 1 Kitasato, Sagamihara, Kanagawa 228 8555, Japan
    Mol Immunol 39:847-54. 2003
    ....
  2. ncbi Nonviral vectors in the new millennium: delivery barriers in gene transfer
    M Nishikawa
    Center for Pharmacogenetics, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15213, USA
    Hum Gene Ther 12:861-70. 2001
    ..Successful clinical applications of nonviral vectors will rely on a better understanding of barriers in gene transfer and development of vectors that can overcome these barriers...
  3. doi Helios Gene Gun-mediated transfection of the inner ear sensory epithelium
    Inna A Belyantseva
    Laboratory of Molecular Genetics, Section on Human Genetics, NIDCD, National Institutes of Health, Rockville MD, USA
    Methods Mol Biol 493:103-23. 2009
    ....
  4. doi High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants
    Elibio L Rech
    Embrapa Genetic Resources and Biotechnology, Laboratory of Gene Transfer, Biotechnology Unit, Parque Estação Biológica PqEB W5 Norte, Brasília 70770 900, DF, Brazil
    Nat Protoc 3:410-8. 2008
    ..the herbicide imazapyr as a selectable marker, multiple shoot induction from embryonic axes of mature seeds and biolistics techniques...
  5. ncbi The myth of plant transformation
    Abdul Qayyum Rao
    National Centre of Excellence in Molecular Biology, 87 West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan
    Biotechnol Adv 27:753-63. 2009
    ..This review provides a comprehensive discussion of important issues related to plant transformation as well as advances made in transformation techniques during three decades...
  6. ncbi Transfection by particle bombardment: delivery of plasmid DNA into mammalian cells using gene gun
    Masaki Uchida
    The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N1AX, UK
    Biochim Biophys Acta 1790:754-64. 2009
    ..Recently, particle bombardment has become increasingly popular as a transfection method, because of a reduced dependency on target cell characteristics. In this study, we evaluated in vitro gene transfer by particle bombardment...
  7. ncbi Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles
    Paul A Raju
    The PowderJect Centre for Gene and Drug Delivery Research, University of Oxford, 43 Banbury Rd, Oxford OX2 6PE, UK
    Vaccine 24:4644-7. 2006
    ..96+/-5.22, 45.91+/-10.89, 90.52+/-12.28, respectively. These results suggest that optimization of transfection by genes administered with gene guns is - among other effects - a compromise of micro-particle payload and cell death...
  8. ncbi DNA vaccine against Friend erythroleukaemia virus
    P Sima
    Institute of Haematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic
    Folia Biol (Praha) 48:43-50. 2002
    ..The FV type A or P was used as a challenge. The immunization with gag- and env-derived vaccines resulted in protective immunity in a high proportion of mice...
  9. pmc An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm
    Miles R Armstrong
    Plant Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, UK
    Proc Natl Acad Sci U S A 102:7766-71. 2005
    ..We suggest that Avr3a has undergone gene duplication and that an allele evading recognition by R3a arose under positive selection...
  10. ncbi Downregulation of IgE antibody and allergic responses in the lung by epidermal biolistic microparticle delivery
    Mark Kendall
    The PowderJect Centre for Gene and Drug Delivery Research, University of Oxford, UK
    J Allergy Clin Immunol 117:275-82. 2006
    ..The precision of the injection preferentially targets the Langerhans cell network, which, although ideal for vaccination, might not be suitable for the downregulation of immune responses in immunotherapy...
  11. pmc Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles
    JOHN A O'BRIEN
    Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    BMC Biotechnol 11:66. 2011
    ..Here we describe the use of 40 nm diameter projectiles--nanoparticles--in biolistic transfections to determine if they are a suitable alternative to microparticles...
  12. ncbi Comparative efficacy of the Schistosoma mansoni nucleic acid vaccine, Sm23, following microseeding or gene gun delivery
    Akram A Da'Dara
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Parasite Immunol 24:179-87. 2002
    ..However, the protective effect of the vaccine was not affected by coadministering plasmids encoding either IL-12 or IL-4 using the microseeding technique...
  13. ncbi Comparison of HPV DNA vaccines employing intracellular targeting strategies
    J W Kim
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    Gene Ther 11:1011-8. 2004
    ..Our data suggest that the DNA vaccine linking CRT to E7 (CRT/E7) may be a suitable candidate for human trials for the control of HPV infections and HPV-associated lesions...
  14. pmc Biolistic transfection of neuronal cultures using a hand-held gene gun
    JOHN A O'BRIEN
    Neurobiology Division, Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Protoc 1:977-81. 2006
    ..The method can be readily adapted to other cell types and tissues. The protocol can be completed in 1-2 h...
  15. doi Fusion of a viral antigen to invariant chain leads to augmented T-cell immunity and improved protection in gene-gun DNA-vaccinated mice
    Mirjana Grujic
    Institute of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
    J Gen Virol 90:414-22. 2009
    ..Furthermore, given the fact that the Ii chain enhances the presentation of more than one epitope, this suggests that Ii-chain-based DNA vaccines may be promising candidates for various heterologous prime-boost regimes...
  16. ncbi Gene gun bombardment with gold particles displays a particular Th2-promoting signal that over-rules the Th1-inducing effect of immunostimulatory CpG motifs in DNA vaccines
    Richard Weiss
    Institute of Chemistry and Biochemistry, University of Salzburg, Hellbrunner Street 34, A 5020 Salzburg, Austria
    Vaccine 20:3148-54. 2002
    ..Furthermore, the data also indicate a strong and even systemic adjuvant effect of the gene gun shot itself...
  17. ncbi A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques
    S Travella
    John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
    Plant Cell Rep 23:780-9. 2005
    ..For the efficient production of transgenic barley plants, with stable transgene expression and reduced silencing, the Agrobacterium-mediated method appears to offer significant advantages over particle bombardment...
  18. ncbi Imaging calcium dynamics in the nervous system by means of ballistic delivery of indicators
    Petronella Kettunen
    Department of Neuroscience, Nobel Institute for Neurophysiology, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Neurosci Methods 119:37-43. 2002
    ..The ballistic delivery of calcium indicators thus opens up many new avenues for further exploration of the structure and function of the nervous system from single spines to neuronal networks...
  19. ncbi Microtargeted gene silencing and ectopic expression in live embryos using biolistic delivery with a pneumatic capillary gun
    Orit Shefi
    Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0376, USA
    J Neurosci 26:6119-23. 2006
  20. doi Cationic gold microparticles for biolistic delivery of nucleic acids
    Sergei Svarovsky
    Center for Innovations in Medicine, The Biodesign Institute at Arizona State University, Tempe, AZ 85287, USA
    Biotechniques 45:535-40. 2008
    ..Along with its superior properties as a DNA delivery vehicle, charge-modified gold offers a unique opportunity to deliver various DNA formulations in addition to traditional naked DNA...
  21. ncbi Immunisation with modified HPV16 E7 genes against mouse oncogenic TC-1 cell sublines with downregulated expression of MHC class I molecules
    Michal Smahel
    Department of Experimental Virology, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic
    Vaccine 21:1125-36. 2003
    ..Cell lines with downregulated MHC class I expression derived from TC-1 cells may represent a useful model for testing therapeutic anti-HPV vaccines in settings more relevant to clinical requirements...
  22. ncbi Enhancement of immunogenicity of HPV16 E7 oncogene by fusion with E. coli beta-glucuronidase
    Michal Smahel
    Institute of Hematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic
    J Gene Med 6:1092-101. 2004
    ..In previous work, we prepared the E7GGG gene containing point mutations resulting in substitution of three amino acids in the pRb-binding site of the HPV16 E7 protein...
  23. ncbi Topical delivery of imiquimod to a mouse model as a novel adjuvant for human immunodeficiency virus (HIV) DNA
    Anne Kjerrström Zuber
    Swedish Institute for Infectious Disease Control, SE 17182 Stockholm, Sweden
    Vaccine 22:1791-8. 2004
    ..The delivery of an adjuvant as a topical cream rather than through injections has a clear clinical benefit. We show for the first time that imiquimod can act as an adjuvant for DNA vaccination...
  24. pmc Enhancing DNA vaccine potency by coadministration of DNA encoding antiapoptotic proteins
    Tae Woo Kim
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Clin Invest 112:109-17. 2003
    ..Thus, coadministration of DNA vaccines with DNA encoding antiapoptotic proteins represents an innovative approach to enhance DNA vaccine potency...
  25. ncbi Improving DNA vaccine potency via modification of professional antigen presenting cells
    Chien Fu Hung
    Department of Pathology, Johns Hopkins University School of Medicine, Ross 512H, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Curr Opin Mol Ther 5:20-4. 2003
    ....
  26. ncbi Imiquimod and resiquimod in a mouse model: adjuvants for DNA vaccination by particle-mediated immunotherapeutic delivery
    Lindy L Thomsen
    LindyDepartment of Immunotherapeutics, GlaxoSmithKline Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK
    Vaccine 22:1799-809. 2004
    ..Collectively, these studies suggest that both imiquimod and resiquimod may be suitable adjuvants for therapeutic DNA vaccines requiring induction of potent cytotoxic T cell responses...
  27. ncbi Transient expression and insertional mutagenesis of Puccinia triticina using biolistics
    Craig A Webb
    USDA ARS, Plant Science and Entomology Research Unit, Kansas State University, Manhattan, KS 66506 5502, USA
    Funct Integr Genomics 6:250-60. 2006
    ..Using a linearized cloning plasmid, stable integration into the genome was achieved as demonstrated by PCR and sequencing analysis...
  28. doi Promotion of flowering and reduction of a generation time in apple seedlings by ectopical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector
    Noriko Yamagishi
    Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3 18 8, Morioka, 020 8550, Japan
    Plant Mol Biol 75:193-204. 2011
    ....
  29. pmc A helium burst biolistic device adapted to penetrate fragile insect tissues
    J L Thomas
    Unité Nationale Séricicole, INRA, 25 quai J J Rousseau, 69350 La Mulatière, France
    J Insect Sci 1:9. 2001
    ..No significant differences were noted between these two modes of delivery. The major improvement is the possibility of high pressure shooting correlated with appreciable penetration and a weak blast effect...
  30. ncbi Transfection of liver in vivo by biolistic particle delivery: its use in the investigation of cytochrome P450 gene regulation
    Roongsiri Muangmoonchai
    Department of Biochemistry and Molecular Biology, University College London, UK
    Mol Biotechnol 20:145-51. 2002
    ..However, when attached to the phenobarbital response element both promoters give the same fold-induction of reporter activity in response to phenobarbital...
  31. pmc CpG motif acts as a 'danger signal' and provides a T helper type 1-biased microenvironment for DNA vaccination
    Ling Liu
    Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical College, Fudan University, Shanghai, China
    Immunology 115:223-30. 2005
    ..These results may help to explain the mechanism of different types of immune response induced by DNA vaccines delivered by different routes and facilitate the application of DNA vaccines...
  32. doi Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer
    Chi Chen Lin
    Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    J Gene Med 10:679-89. 2008
    ..To test whether this gun could inoculate a DNA vaccine that stimulates an anti-tumor Th1 immune response, we examined the effect of direct delivery of naked DNA (i.e. without any carrier) on the anti-tumor immune response of mice...
  33. ncbi Nonviral gene gun mediated transfer into the beating heart
    Yukihiro Matsuno
    First Department of Surgery, Gifu University School of Medicine, Gifu, Japan
    ASAIO J 49:641-4. 2003
    ..This technique is effective for gene transfer into the heart and may be one of the most useful methods for gene therapy for many cardiovascular diseases in the future...
  34. ncbi Activation of systemic acquired silencing by localised introduction of DNA
    J C Palauqui
    Laboratoire de Biologie Cellulaire, INRA Centre de Versailles F 78026, Versailles, Cedex, France
    Curr Biol 9:59-66. 1999
    ..Graft-transmitted silencing is maintained in class II but not in class I plants when removed from silenced stocks, indicating similar requirements for spontaneous triggering and maintenance...
  35. doi Transfection of rat or mouse neurons by biolistics or electroporation
    Sulayman D Dib-Hajj
    Department of Neurology, School of Medicine, Yale University, New Haven, CT, USA
    Nat Protoc 4:1118-26. 2009
    ..Although we have used sodium channels for the examples that we show here, these methods can also be used to study other types of molecules...
  36. ncbi Biolistic delivery of Ca2+ dyes into plant and algal cells
    John H F Bothwell
    Marine Biological Association of the UK, The Laboratory, Citadel Hill, Plymouth PL1 2PB, UK
    Plant J 46:327-35. 2006
    ..Biolistic loading of differentiated plant cells is easier, quicker, and more widely applicable than micro-injection, and should broaden the study of plant signal transduction...
  37. ncbi DNA immunization of mice with a plasmid encoding Neisseria gonorrhea PorB protein by intramuscular injection and epidermal particle bombardment
    W Zhu
    Department of Medicine, Division of Infectious Diseases, University of North Carolina at Chapel Hill, CB 7031, 8431 Medicine and Biomolecular Research Building, Chapel Hill, NC 27599, USA
    Vaccine 22:660-9. 2004
    ..Based on different prime-boost protocols, we could polarize immune responses to predominantly Th1 or Th2, which should enable future studies of the types of immune responses that are protective in mouse models of gonorrhea...
  38. ncbi Targeting the vaccinia virus L1 protein to the cell surface enhances production of neutralizing antibodies
    Joseph W Golden
    Department of Molecular Virology, Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, United States
    Vaccine 26:3507-15. 2008
    ..Furthermore, our results demonstrate that a DNA-based vaccine is capable of establishing protection from lethal orthopoxvirus challenges when administered as a prime and single boost without requiring adjuvant...
  39. ncbi Direct transfection and activation of human cutaneous dendritic cells
    A T Larregina
    Department of Dermatology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA
    Gene Ther 8:608-17. 2001
    ..Taken together, our results demonstrate direct transfection, activation, and antigen-specific stimulatory function of in situ transduced human Langerhans cells...
  40. ncbi The effects of gene gun delivered pIL-3 adjuvant on skin pathology and cytokine expression
    K Matthews
    Centre of Infectious Diseases, Royal Dick School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK
    Vet Immunol Immunopathol 119:233-42. 2007
    ..pIL-3 did not affect the expression of the immune response polarizing cytokines, IL-10 and IL-12...
  41. ncbi Immunization by particle-mediated transfer of the granulocyte-macrophage colony-stimulating factor gene into autologous tumor cells in melanoma or sarcoma patients: report of a phase I/IB study
    D M Mahvi
    Department of Surgery, University of Wisconsin School of Medicine, Madison, WI 53792, USA
    Hum Gene Ther 13:1711-21. 2002
    ..This technique of gene transfer was safe and feasible, but resulted in clinically relevant levels of gene expression in only a minority of patients...
  42. ncbi Nonviral approaches for targeted delivery of plasmid DNA and oligonucleotide
    Shigeru Kawakami
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Sci 97:726-45. 2008
    ..In this review, we will focus on the in vivo targeted delivery of pDNA and ODN using nonviral carriers...
  43. ncbi The diversity of ganglion cells in a mammalian retina
    Rebecca L Rockhill
    Howard Hughes Medical Institute, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Neurosci 22:3831-43. 2002
    ..Some known physiological types could easily be mapped onto structural types, but half of them could not; a large set of poorly known codings of the visual input is transmitted to the brain...
  44. pmc Genetic vaccination against malaria infection by intradermal and epidermal injections of a plasmid containing the gene encoding the Plasmodium berghei circumsporozoite protein
    R Weiss
    Immunology Group, Institute of Chemistry and Biochemistry, University of Salzburg, A 5020 Salzburg, Austria
    Infect Immun 68:5914-9. 2000
    ..The results indicate that a gene gun-mediated CS-specific Th2-type response may be best for protecting against malarial sporozoite infection when the route of parasite entry is via mosquito bite...
  45. pmc Organization of spines on the dendrites of Purkinje cells
    JOHN O'BRIEN
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 103:1575-80. 2006
    ..The purpose of this organization may therefore be to maximize the opportunity of different spines to interact with different axons...
  46. ncbi Removal of the circumsporozoite protein (CSP) glycosylphosphatidylinositol signal sequence from a CSP DNA vaccine enhances induction of CSP-specific Th2 type immune responses and improvesprotection against malaria infection
    S Scheiblhofer
    Immunology Group, Institute of Chemistry and Biochemistry, University of Salzburg, Salzburg, Austria
    Eur J Immunol 31:692-8. 2001
    ..Engineering plasmid constructs for proper cellular localization of gene products is a primary consideration for the preparation of optimally efficacious DNA vaccines...
  47. pmc Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues
    Jean Luc Thomas
    UNS INRA, 25 quai J J Rousseau, 69350 La Mulatière, France
    J Insect Sci 3:17. 2003
    There are few powerful techniques available to transfect insect tissues. We previously used biolistics to transfect Bombyx mori embryos, and larval and pupal tissues (Thomas J-L et al. 2001...
  48. doi Biolistics transformation of wheat
    Caroline A Sparks
    Centre for Crop Genetic Improvement, Department of Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, UK
    Methods Mol Biol 478:71-92. 2009
    ....
  49. ncbi Cross-reactive immune responses in mice after genetic vaccination with cDNA encoding hantavirus nucleocapsid proteins
    Marie Lindkvist
    Department of Medical Countermeasures, Division of NBC Defence, Swedish Defence Research Agency, SE 901 82 Umea, Sweden
    Vaccine 25:1690-9. 2007
    ..Our experimental data in combination with B-cell prediction software indicate that strong homologous virus species specific and cross-reactive epitopes are located around amino acid residue 40 in the nucleocapsid proteins...
  50. ncbi Immunologic response to xenogeneic gp100 DNA in melanoma patients: comparison of particle-mediated epidermal delivery with intramuscular injection
    Brian A Ginsberg
    Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10065, USA
    Clin Cancer Res 16:4057-65. 2010
    ..To further define the optimal vaccination strategy, we conducted a pilot clinical trial comparing i.m. injection with particle-mediated epidermal delivery (PMED)...
  51. ncbi Dynamics modelling of biolistic gene guns
    Mingjun Zhang
    Life Science and Chemical Analysis Division, Agilent Technologies, Santa Clara, CA 95051, USA
    Phys Med Biol 52:1485-93. 2007
    ..Simulations have also been conducted. The results agree well with experimental results in the open literature. The contribution of this paper includes a dynamic model for improving and manipulating performance of the biolistic gene gun...
  52. ncbi Efficient transformation of Dictyostelium discoideum with a particle inflow gun
    B Wetterauer
    Zoologisches Institut der Universität München, Munich, Germany
    Biochim Biophys Acta 1499:139-143. 2000
    ..This is five times more than we achieve with the same vector using electroporation protocols. We also show that the particle inflow gun can be used for analysis of developmentally regulated gene expression in a transient assay...
  53. ncbi The delivery of PEBBLE nanosensors to measure the intracellular environment
    A Webster
    School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK
    Biochem Soc Trans 35:538-43. 2007
    ..This paper will overview several of the most popular methods used for the introduction of PEBBLE nanosensors to the cellular environment and discuss the efficacy of the techniques...
  54. ncbi PCR-generated linear DNA fragments utilized as a hantavirus DNA vaccine
    P Johansson
    Department of Medical Countermeasures, Division of NBC Defence, Swedish Defence Research Agency, SE 90182 Umeå, Sweden
    Vaccine 20:3379-88. 2002
    ....
  55. ncbi The biolistic method as a tool for testing the differential activity of putative silkmoth chorion gene promoters
    L Kravariti
    Department of Biochemistry and Molecular Biology, University of Athens, Panepistimioupolis, 15701, Athens, Greece
    Insect Biochem Mol Biol 31:473-9. 2001
    ..functional study of the tissue and temporal specificity of the 6F6 putative promoter region, we decided to apply biolistics. In the present work, use of a region from the 6F6...
  56. ncbi Protection of mice against challenge with Bacillus anthracis STI spores after DNA vaccination
    Ulrike K Hahn
    Institut für Umwrelt und Tierhygiene, University of Hohenheim, Garbenstr 30, Stuttgart 70599, Germany
    Int J Med Microbiol 294:35-44. 2004
    ..Vaccination with either pSecTag PA83 or pCMV/ER PA83 showed significant protection of A/J mice against infection with B. anthracis STI spores...
  57. ncbi Prevention of long-term IgE antibody production by gene gun-mediated DNA vaccination
    Isis Ludwig-Portugall
    Clinical Research Unit of Allergology, Department of Dermatology, Johannes Gutenberg University, Mainz, Germany
    J Allergy Clin Immunol 114:951-7. 2004
    ..Vaccination with allergen-encoding DNA represents a promising approach for the treatment of allergic diseases...
  58. ncbi Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus
    Ning Sun Yang
    Institute of BioAgricultural Sciences, Academia Sinica, Taipei, 11529, Taiwan, ROC
    J Gene Med 7:708-17. 2005
    ..Foot-and-mouth disease virus (FMDV) causes a severe livestock disease, and the virus is an interesting target for virology and vaccine studies...
  59. ncbi CD8+ T-cell-dependent immunity following xenogeneic DNA immunization against CD20 in a tumor challenge model of B-cell lymphoma
    Maria Lia Palomba
    Department of Medicine and Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Clin Cancer Res 11:370-9. 2005
    ..These results show that active immunization with xenogeneic DNA vaccines can induce CD8+ T cell-dependent immunity against CD20...
  60. ncbi DNA vaccination of pigs with open reading frame 1-7 of PRRS virus
    Annette Malene Barfoed
    Danish Institute for Food and Veterinary Research, Lindholm, DK 4771 Kalvehave, Denmark
    Vaccine 22:3628-41. 2004
    ..Pigs vaccinated with ORF1 or ORF4 were primed for antibody response against NSP2 or GP4, respectively. Neutralising antibodies were detected in all pigs, with ORF5 vaccinated pigs showing the highest titres...
  61. ncbi Bystander help within a polyepitope DNA vaccine improves immune responses to influenza antigens
    M Baird
    Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand
    Scand J Immunol 60:363-71. 2004
    ..We conclude that a 'naked DNA' polyepitope vaccine generates specific responses to constituent epitopes and that adjacent irrelevant epitopes may enhance these responses...
  62. ncbi Influence of routes and administration parameters on antibody response of pigs following DNA vaccination
    Annette Malene Barfoed
    Laboratory of Virology and Immunology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 7, DK 1870 Frederiksberg C, Denmark
    Vaccine 22:1395-405. 2004
    ..m.)/intradermal (i.d.) injection of plasmid DNA. This indicates, that the ear is an attractive site for gene gun vaccination of pigs...
  63. ncbi Combined immunization with fusion genes of mutated E7 gene of human papillomavirus type 16 did not enhance antitumor effect
    Dana Pokorna
    Institute of Hematology and Blood Transfusion, Department of Experimental Virology, U Nemocnice 1, 128 20 Prague 2, Czech Republic
    J Gene Med 7:696-707. 2005
    ..Its immunogenicity has been substantially enhanced by fusion with several proteins of various origins and functions. Different mechanisms have been responsible for increased vaccination efficacy of fusion proteins...
  64. ncbi Effect of cytokine-encoding plasmid delivery on immune response to Japanese encephalitis virus DNA vaccine in mice
    Kaushik Bharati
    National Institute of Immunology, New Delhi, India
    Microbiol Immunol 49:349-53. 2005
    ..However, intra-dermal co-administration of pMEa and pGM-CSF in mice using the gene gun, enhanced anti-JEV antibody titers resulting in an increased level of protection in mice against lethal JEV challenge...
  65. ncbi B lymphocytes participate in cross-presentation of antigen following gene gun vaccination
    Huiming Hon
    Department of Microbiology and Immunology, Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA
    J Immunol 174:5233-42. 2005
    ..Collectively, these data indicate that broad expression of the immunogen is required for optimal induction of protective acquired immunity...
  66. ncbi Immunologic analysis induced by DNA vaccine encoding E protein of Beijing-1 strain derived from Japanese encephalitis virus
    Guo he Feng
    Department of Infectious Diseases, Second Affiliated Hospital of China Medical University, Shenyang, China
    Intervirology 50:93-8. 2007
    ..This study aimed to construct a recombinant encoding E protein of the Beijing-1 strain derived from Japanese encephalitis virus and analyze the humoral, cellular and protective immunity induced by the above recombinant...
  67. pmc Suppression of major histocompatibility complex class II-associated invariant chain enhances the potency of an HIV gp120 DNA vaccine
    Xueqing Lu
    Antigen Express, Inc, Worcester, MA 01606 2758, USA
    Immunology 120:207-16. 2007
    ..Activated CD4(+) T cells locally strengthen the response of CD8(+) CTL, thus enhancing the potency of a DNA vaccine...
  68. ncbi Gene gun immunization with clinically relevant allergens aggravates allergen induced pathology and is contraindicated for allergen immunotherapy
    Sandra Scheiblhofer
    Christian Doppler Laboratory for Allergy Diagnosis and Therapy, Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria
    Mol Immunol 44:1879-87. 2007
    ....
  69. ncbi Comparative evaluation of CD8+CTL responses following gene gun immunization targeting the skin with intracutaneous injection of antigen-transduced dendritic cells
    Evelyn Gaffal
    Laboratory of Experimental Dermatology, Department of Dermatology, University of Bonn, Sigmund Freud Street 25, D 53105 Bonn, Germany
    Eur J Cell Biol 86:817-26. 2007
    ..Further investigations will have to address the role of Langerhans cells for the activation of cellular immunity in the skin...
  70. ncbi Evaluation of a needle-free delivery platform for prime-boost immunization with DNA and modified vaccinia virus ankara vectors expressing herpes simplex virus 2 glycoprotein D
    Clement A Meseda
    Laboratory of DNA Viruses, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20852, USA
    Viral Immunol 19:250-9. 2006
    ..Thus, needle-free delivery of plasmid DNA may provide additional flexibility and options for effective prime-boost vaccination...
  71. ncbi Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa
    Sukumar Saha
    Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Vaccine 24:6240-9. 2006
    ..These results implied the possible clinical application of multivalent DNA vaccine against P. aeruginosa...
  72. ncbi Essential sequence of influenza neuraminidase DNA to provide protection against lethal viral infection
    Xiangzhong Li
    College of Life Sciences, Hunan Normal University, Changsha, Hunan, People s Republic of China
    DNA Cell Biol 25:197-205. 2006
    ..The nt58-1299 of NA DNA may play an important role in protection against influenza virus...
  73. pmc Abeta42 gene vaccination reduces brain amyloid plaque burden in transgenic mice
    Baoxi Qu
    Alzheimer s Diseases Center, Department of Neurology, University of Texas Southwestern Medical Center, 5323, Harry Hines, Dallas, 75390 9036, USA
    J Neurol Sci 244:151-8. 2006
    ..To demonstrate that in APPswe/PS1DeltaE9 transgenic mice, gene gun mediated Abeta42 gene vaccination elicits a high titer of anti-Abeta42 antibodies causal of a significant reduction of Abeta42 deposition in brain...
  74. ncbi The involvement of immunoproteasomes in induction of MHC class I-restricted immunity targeting Toxoplasma SAG1
    Kazunari Ishii
    Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Microbes Infect 8:1045-53. 2006
    ....
  75. ncbi Analysis of humoral immunity of hepatitis D virus DNA vaccine generated in mice by using different dosage, gene gun immunization, and in vivo electroporation
    Yih Tsong Shiau
    Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taiwan, ROC
    J Chin Med Assoc 69:7-13. 2006
    ..Whether the low humoral immunity of L-HDAg DNA vaccine is due to inadequate dosage or can be ameliorated by other modes of immunization needs further evaluation...
  76. ncbi Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma
    Arianna Smorlesi
    Laboratory of Tumor Immunology, Immunology Center, INRCA Gerontology Research Department, Via Birarelli 8, 60121 Ancona, Italy
    Vaccine 24:1766-75. 2006
    ....
  77. ncbi DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)
    Dana Pokorna
    Department of Experimental Virology, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic
    Oncol Rep 14:1045-53. 2005
    ..Their antitumor effect was comparable to those of the whole E7 gene and Sig/E7GGG/LAMP-1 fusion gene. There was no relevant difference between immune responses elicited by CP-E7 and CPdel-E7 DNA vaccination...
  78. ncbi Gene-gun biolistic immunization encoding glutamic acid decarboxylase: a model for studying Langerhans cell abnormalities and mimicry in the nonobese diabetic mouse
    Béatrice Joussemet
    Immuno endocrinology Unit, ENVN INRA University, ENVN, Atlanpole La Chantrerie, BP 40706, 44307 Nantes Cedex 03, France
    Ann N Y Acad Sci 1051:613-25. 2005
    ..The gene-gun technique could thus provide an interesting model for studying skin DC abnormalities in NOD mice and their potential implication of presenting mimetic peptides that modulate the autoimmune response in T1D...
  79. ncbi pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infection
    Adriana Malheiro
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av Café S N, 14040 903 Ribeirao Preto, SP, Brazil
    Vaccine 26:305-15. 2008
    ..canis infection, suggesting their possible use in further combined therapeutic interventions...
  80. ncbi [Construction of DNA vaccine of Trichinella spiralis muscle larvae and its expression in mice]
    Jing Cui
    Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22:5-8. 2004
    ..To construct and express recombinant plasmid containing the structural gene encoding Mr 31 000 antigen of Trichinella spiralis muscle larvae...
  81. ncbi Gene transfer methods for CNS organotypic cultures: a comparison of three nonviral methods
    R C Murphy
    Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002, USA
    Mol Ther 3:113-21. 2001
    Organotypic slice cultures from postnatal day 12 mouse cerebellum were transfected using three nonviral methods: biolistics (gene gun), lipotransfection, and electroporation...
  82. ncbi Individual analysis of mice vaccinated against a weakly immunogenic self tumor-specific antigen reveals a correlation between CD8 T cell response and antitumor efficacy
    Antonio Rosato
    Immunology Section, Department of Oncology and Surgical Sciences, University of Padua, Padua, Italy
    J Immunol 171:5172-9. 2003
    ..These results highlight the critical need to identify reliable, specific immunological parameters that may predict success or failure of an immune response against cancer...
  83. ncbi Induction of murine ras oncogene peptide-specific T cell responses by immunization with plasmid DNA-based minigene vectors
    Peter Lindinger
    Immunology Group, Institute of Chemistry and Biochemistry, University of Salzburg, Salzburg, Austria
    Vaccine 21:4285-96. 2003
    ....
  84. ncbi Comparison of individual and combination DNA vaccines for B. anthracis, Ebola virus, Marburg virus and Venezuelan equine encephalitis virus
    Jenny Riemenschneider
    Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702 5011, USA
    Vaccine 21:4071-80. 2003
    ....
  85. ncbi Comparison of the CD8+ T cell responses and antitumor effects generated by DNA vaccine administered through gene gun, biojector, and syringe
    Cornelia Trimble
    Department of Obstetrics and Gynecology, The Johns Hopkins Medical Institution, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Vaccine 21:4036-42. 2003
    ..m.) and biojector administrations. Thus, our data suggest that DNA vaccination via gene gun represents the most potent regimen for DNA administration...
  86. ncbi [Antitumor effect of gene gun-mediated DNA vaccine pWRG-neu immunization in C57BL/6 mice]
    Yuan Yuan Zha
    National Laboratory of Molecular Oncology, Cancer Institute Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China
    Zhonghua Zhong Liu Za Zhi 25:320-4. 2003
    ..To investigate the inhibitory effect of DNA vaccine immunization on neu-overexpressed melanoma growth in prophylactic treatment and anti-lung-metastasis experiments in C57BL/6 mice...
  87. ncbi DNA vaccines, combining form of antigen and method of delivery to raise a spectrum of IFN-gamma and IL-4-producing CD4+ and CD8+ T cells
    Alp E Oran
    Emory Vaccine Center and Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA
    J Immunol 171:1999-2005. 2003
    ..Studies in B cell knockout and hen egg lysozyme Ig transgenic mice revealed that B cells were required for the generation of IL-4-producing CD8(+) T cells...
  88. ncbi Vaccination of rhesus macaques against dengue-2 virus with a plasmid DNA vaccine encoding the viral pre-membrane and envelope genes
    Robert Putnak
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
    Am J Trop Med Hyg 68:469-76. 2003
    ..Long-term protection following DNA vaccination may require revaccination, higher doses of DNA, or a vaccine that contains additional epitopes or adjuvants...
  89. ncbi Lipopeptides: adjuvanticity in conventional and genetic immunization
    K Mittenbühler
    Institut für Molekulare Medizin und Zellforschung, Universitatsklinikum Freiburg, AK Tumorimmunologie Vakzine, Stefan Meier Str 8, D 79104 Freiburg, Germany
    FEMS Immunol Med Microbiol 37:193-200. 2003
    ..We monitored a lipopeptide-dependent shift from a Th1-type to Th2-type response, when DNA immunization was followed by i.p. administration of the lipopeptide adjuvant...
  90. ncbi The protective efficacy of MSP4/5 against lethal Plasmodium chabaudi adami challenge is dependent on the type of DNA vaccine vector and vaccination protocol
    A Rainczuk
    Department of Biochemistry and Molecular Biology, The Cooperative Research Centre for Vaccine Technology, Clayton 3800, Australia
    Vaccine 21:3030-42. 2003
    ..The results suggest that the enhancement of survival against lethal blood-stage malaria challenge after utilizing MSP4/5 DNA vaccination is therefore highly dependent on the route and type of vaccine vector employed...
  91. ncbi Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genes
    Rabie M Mohamed
    Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
    Vaccine 21:2852-61. 2003
    ..gondii organisms compared with 50 microg of T.g.HSP70 gene vaccination by intramuscular (i.m.) or intraperitoneal (i.p.) injection. The vaccine effects of T.g.HSP70 gene persisted for more than 3 months...
  92. ncbi Drug-inducible, dendritic cell-based genetic immunization
    Laura Timares
    Center for Biomedical Inventions, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Immunol 170:5483-90. 2003
    ..Our results indicate that direct transfection is a predominant pathway for LC Ag loading. The ability to regulate transgene expression with inducible DC-based vaccines demonstrates a new level of immunological control...
  93. ncbi Gene gun-based co-immunization of merozoite surface protein-1 cDNA with IL-12 expression plasmid confers protection against lethal Plasmodium yoelii in A/J mice
    Tohru Sakai
    Department of Parasitology and Immunology, University of Tokushima School of Medicine, Japan
    Vaccine 21:1432-44. 2003
    ..yoelii. The results indicate that combined vaccination with DNA encoding antigenic peptides plus IL-12 DNA provides a strategy for improving the prophylactic efficacy of a vaccine for malaria infection...
  94. ncbi Simultaneous GeneGun immunisation with plasmids encoding antigen and GM-CSF: significant enhancement of murine antivenom IgG1 titres
    R A Harrison
    Alistair Reid Venom Research Unit, Division of Molecular Biology and Immunology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
    Vaccine 20:1702-6. 2002
    ..This report illustrates that DNA immunisation protocols to achieve high-titre, venom toxin-specific antibody production are well advanced and encourage the development of a DNA-based approach to antivenom production...
  95. ncbi Expression library immunization confers partial protection against Chlamydia muridarum genital infection
    Celia L McNeilly
    School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU 7XH, Surrey, UK
    Vaccine 25:2643-55. 2007
    ..These results demonstrate that the C. muridarum genome can be screened for individual vaccine candidates by genetic immunization and that the screen produces novel and partially protective vaccine candidates...
  96. ncbi DNA immunization of the grafted liver by particle-mediated gene gun
    Masahiko Nakamura
    Division of Organ Replacement Research and Molecular Immunology, Jichi Medical School, Tochigi, Japan
    Transplantation 76:1369-75. 2003
    ..Direct DNA vaccination of liver allografts before transplantation may provide an effective strategy for inducing protective immunity to infection and malignancy...
  97. ncbi Induction of anti-hepatitis B virus immune responses through DNA immunization
    Michael Geissler
    Department of Internal Medicine II, University of Freiburg, Germany
    Methods Mol Med 96:43-54. 2004
  98. ncbi Schistosoma mansoni miracidia transformed by particle bombardment infect Biomphalaria glabrata snails and develop into transgenic sporocysts
    Oliver Heyers
    Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin, 10115 Berlin, Germany
    Exp Parasitol 105:174-8. 2003
    ..Our findings indicated that it is feasible to return transgenic miracidia to the life cycle, a crucial step for the establishment of a transgenesis system for schistosomes...
  99. pmc Ubiquitin-fusion degradation pathway plays an indispensable role in naked DNA vaccination with a chimeric gene encoding a syngeneic cytotoxic T lymphocyte epitope of melanocyte and green fluorescent protein
    Manxin Zhang
    Department of Microbiology and Immunology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Immunology 112:567-74. 2004
    ..It is noteworthy to document that genetic immunization with pGFP plus pTRP-2(181-188) failed to exert the antitumour immunity...
  100. ncbi Particle-mediated DNA vaccine delivery to the skin
    Joel R Haynes
    PowderJect Vaccines, Inc, 8551 Research Way, Middleton, WI 53562, USA
    Expert Opin Biol Ther 4:889-900. 2004
    ..Particle-mediated DNA vaccines represent a promising tool for developing candidate vaccines against some of the more difficult infectious, parasitic and oncologic disease targets...
  101. ncbi Priming of piglets against enterotoxigenic E. coli F4 fimbriae by immunisation with FAEG DNA
    T Verfaillie
    Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, University Ghent, Salisburylaan 133, Merelbeke B 9820, Belgium
    Vaccine 22:1640-7. 2004
    ..The low responses to the pcDNA1/faeG19 DNA vaccination suggest that delivery of the DNA and/or the expression of the faeG gene should be improved...

Research Grants108 found, 100 shown here

  1. DNA Immunizations with GAD65 to Induce Tolerance
    PENELOPE MOREL; Fiscal Year: 2002
    ..abstract_text> ..
  2. Microglial Neuroprotection and Neurotoxicity
    Greer Murphy; Fiscal Year: 2005
    ..of M-CSFR on microglia protects neurons from excitotoxic injury in slice cultures transfected using biolistics, and in a microglial-hippocampal organotypic co-culture system...
  3. Microglial Neuroprotection and Neurotoxicity
    Greer Murphy; Fiscal Year: 2006
    ..of M-CSFR on microglia protects neurons from excitotoxic injury in slice cultures transfected using biolistics, and in a microglial-hippocampal organotypic co-culture system...
  4. Microglial Neuroprotection and Neurotoxicity
    Greer Murphy; Fiscal Year: 2007
    ..of M-CSFR on microglia protects neurons from excitotoxic injury in slice cultures transfected using biolistics, and in a microglial-hippocampal organotypic co-culture system...
  5. Microglial Neuroprotection and Neurotoxicity
    Greer Murphy; Fiscal Year: 2004
    ..of M-CSFR on microglia protects neurons from excitotoxic injury in slice cultures transfected using biolistics, and in a microglial-hippocampal organotypic co-culture system...
  6. Microglial Neuroprotection and Neurotoxicity
    Greer Murphy; Fiscal Year: 2003
    ..of M-CSFR on microglia protects neurons from excitotoxic injury in slice cultures transfected using biolistics, and in a microglial-hippocampal organotypic co-culture system...
  7. High-Efficiency Biolistic Device for Brain Transfection
    DONALD LO; Fiscal Year: 2005
    device, based on particle-mediated gene transfer (or 'biolistics'), for the identification and validation of candidate therapeutic drugs and targets...
  8. High-Efficiency Biolistic Device for Brain Transfection
    DONALD LO; Fiscal Year: 2006
    device, based on particle-mediated gene transfer (or 'biolistics'), for the identification and validation of candidate therapeutic drugs and targets...
  9. Comparative and functional genomics of C. neoformans
    James Kronstad; Fiscal Year: 2006
    ..We will compare the organization and transcriptional regulation of these genes (and a third iron-regulated gene CnURF1) between serotypes. We will also disrupt the genes to examine their role in virulence. ..
  10. Pharmacogenetic Therapy of Pulmonary Hypertension
    Song Li; Fiscal Year: 2005
    ..g. nitric oxide and oxygen), provide an approach to prevent the progressive pathological aspects of PHT and ameliorate return towards more normal pulmonary vascular structure and function. ..
  11. Experience-Dependent Plasticity Of Synaptic Structure
    Wenbiao Gan; Fiscal Year: 2005
    ..Together, these studies will provide a comprehensive view of the role of experience in shaping synaptic connectivity at different stages of life and suggest potential strategies for functional rehabilitation after brain injury ..
  12. K12 Expression: Cornea-Type Epithelial Differentiation
    WINSTON KAO; Fiscal Year: 2005
    ..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
  13. Roles of Growth Factors on Corneal Morphogenesis
    WINSTON KAO; Fiscal Year: 2003
    ..g., epithelial debridement, incision, alkali burn. ..
  14. In Vivo Study of Synapses in Alzheimer's Disease Models
    Wenbiao Gan; Fiscal Year: 2003
    ....
  15. Pharmacogenetic Therapy of Pulmonary Hypertension
    Song Li; Fiscal Year: 2004
    ..g. nitric oxide and oxygen), provide an approach to prevent the progressive pathological aspects of PHT and ameliorate return towards more normal pulmonary vascular structure and function. ..
  16. K12 Expression: Cornea-Type Epithelial Differentiation
    WINSTON KAO; Fiscal Year: 2004
    ..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
  17. Roles of Growth Factors on Corneal Morphogenesis
    WINSTON KAO; Fiscal Year: 2004
    ..g., epithelial debridement, incision, alkali burn. ..
  18. Retrograde Dystrophic Influence of IFN-gamma on Neurons
    Pamela Lein; Fiscal Year: 2004
    ..abstract_text> ..
  19. In Vivo Study of Synapses in Alzheimer's Disease Models
    Wenbiao Gan; Fiscal Year: 2004
    ....
  20. Therapeutic antibodies for lethal anthrax infection
    John Herrmann; Fiscal Year: 2003
    ..At the end of this phase 1 study, a standardized schedule will be established in phase II to prepare for large-scale production and testing of this much needed, perhaps essential, product in biodefense against anthrax attacks. ..
  21. Links among synaptic activity, translation and structure
    Peter Vanderklish; Fiscal Year: 2003
    ....
  22. Experience-Dependent Plasticity Of Synaptic Structure
    Wenbiao Gan; Fiscal Year: 2006
    ..studies will provide a comprehensive view of the role of experience in shaping synaptic connectivity at different stages of life and suggest potential strategies for functional rehabilitation after brain injury [unreadable] [unreadable]..
  23. Naltrexone as a Novel Treatment for Diabetic Keratopathy
    IAN ZAGON; Fiscal Year: 2007
    ..The proposed use of biotherapy with NTX is an innovative approach whereby the patient's own growth regulatory mechanisms are manipulated to correct complications from diabetes. ..
  24. Immunobiological Role of Fertilization Antigen(FA-1)
    RAJESH NAZ; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  25. Circadian regulation of microRNA biogenesis and function
    Peter Vanderklish; Fiscal Year: 2009
    ..Our studies will define a mechanistic link between circadian rhythm and protein synthesis in neurons, providing fundamental insights into the control of brain function and neurological diseases. ..
  26. Non Viral Gene Therapy for Lung Injury
    Song Li; Fiscal Year: 2010
    ..Public Health Relevance: This study is aimed to develop a novel gene therapy for the treatment of pulmonary injury such as acute respiratory distress syndrome and pulmonary fibrosis. ..
  27. Molecular and Cellular Basis of PCB Developmental Neurotoxicity
    Isaac N Pessah; Fiscal Year: 2010
    ....
  28. Immune molecules in early postnatal nervous system development
    A Kimberley McAllister; Fiscal Year: 2010
    ....
  29. SYNAPSE FORMATION AND REFINEMENT IN THE VISUAL SYSTEM
    A Kimberley McAllister; Fiscal Year: 2010
    ..Understanding the cellular and molecular mechanismsof synapse formation could revolutionize our ability to identify,prevent, and treat these developmental disorders. ..
  30. SYNAPSE FORMATION AND REFINEMENT IN THE VISUAL SYSTEM
    A McAllister; Fiscal Year: 2009
    ..Understanding the cellular and molecular mechanismsof synapse formation could revolutionize our ability to identify,prevent, and treat these developmental disorders. ..
  31. Circadian regulation of microRNA biogenesis and function
    Peter W Vanderklish; Fiscal Year: 2010
    ..Our studies will define a mechanistic link between circadian rhythm and protein synthesis in neurons, providing fundamental insights into the control of brain function and neurological diseases. ..
  32. Molecular and Cellular Basis of PCB Developmental Neurotoxicity
    PAMELA J contact LEIN; Fiscal Year: 2011
    ....
  33. K12 Expression: Cornea-Type Epithelial Differentiation
    WINSTON KAO; Fiscal Year: 2008
    ..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
  34. Skin Cell Culture
    Laura Timares; Fiscal Year: 2008
    ..abstract_text> ..
  35. K12 Expression: Cornea-Type Epithelial Differentiation
    WINSTON KAO; Fiscal Year: 2007
    ..Aim 3 is to examine roles of Fos on corneal-type epithelium differentiation and corneal wound healing as described in Aim 2. ..
  36. Experience-Dependent Plasticity Of Synaptic Structure
    Wenbiao Gan; Fiscal Year: 2007
    ..studies will provide a comprehensive view of the role of experience in shaping synaptic connectivity at different stages of life and suggest potential strategies for functional rehabilitation after brain injury [unreadable] [unreadable]..
  37. Mechanism of Eliciting Antitumor Cell Immunity Via Lentivector Immunization
    Yukai He; Fiscal Year: 2007
    ..The knowledge gained from these studies will assist the design of more effective and safer genetic immunization approaches for antitumor immunotherapy. ..
  38. SURGICAL ONCOLOGY RESEARCH TRAINING PROGRAM
    David Mahvi; Fiscal Year: 2007
    ..Trainees will be provided with the background to organize, develop, and lead translational and clinical surgical oncology research programs in a variety of academic, governmental, or industrial institutions. ..
  39. Nanosized Delivery System for Site-Specific Drug Release
    Song Li; Fiscal Year: 2008
    ..Successful completion of these studies will lead to development of novel liposomal drugs that will advance the treatment of ?PA-positive cancers. ..
  40. Structure/Function Relationship of The Lumican Gene
    WINSTON KAO; Fiscal Year: 2008
    ..g., persistent and excessive inflammation, corneal transparency of injured cornea, improved wound healing of diabetes, etc. ..
  41. Roles of Growth Factors on Corneal Morphogenesis
    WINSTON KAO; Fiscal Year: 2009
    ..Data obtained will yield useful information for a better understanding of corneal diseases at molecular and cellular levels in vivo and serve as basis for designing treatment regiments for corneal wound healing. ..
  42. SYNAPSE FORMATION AND REFINEMENT IN THE VISUAL SYSTEM
    A McAllister; Fiscal Year: 2009
    ..Understanding the cellular and molecular mechanismsof synapse formation could revolutionize our ability to identify,prevent, and treat these developmental disorders. ..
  43. Non Viral Gene Therapy for Lung Injury
    Song Li; Fiscal Year: 2011
    ..Public Health Relevance: This study is aimed to develop a novel gene therapy for the treatment of pulmonary injury such as acute respiratory distress syndrome and pulmonary fibrosis. ..
  44. SYNAPSE FORMATION AND REFINEMENT IN THE VISUAL SYSTEM
    A McAllister; Fiscal Year: 2002
    ..Understanding the cellular and molecular mechanisms of synapse formation and refinement could revolutionize our ability to identify, prevent, and treat these developmental disorders. ..
  45. STRUCTURE/FUNCTION RELATIONSHIP OF LUMICAN
    WINSTON KAO; Fiscal Year: 2001
    ..The resulting homologous recombinant ES cells will be used to prepare knock-in mice, e.g., LumS/S, LumF/F and LumT/T. The phenotypic changes in ECM of such knock- in mice will be examined. ..
  46. NOVEL CD32 ISOFORMS MODULATE NK CELL FUNCTIONS
    PENELOPE MOREL; Fiscal Year: 2001
    ..abstract_text> ..
  47. SYNAPSE FORMATION AND REFINEMENT IN THE VISUAL SYSTEM
    A McAllister; Fiscal Year: 2001
    ..Understanding the cellular and molecular mechanisms of synapse formation and refinement could revolutionize our ability to identify, prevent, and treat these developmental disorders. ..
  48. In Vivo Study of Synapses in Alzheimer's Disease Models
    Wenbiao Gan; Fiscal Year: 2001
    ....
  49. DEVELOPMENT OF ROTAVIRUS DNA VACCINES
    John Herrmann; Fiscal Year: 2001
    ..Immunization with DNA vaccines that express specific rotaviral proteins offers a new approach to vaccination against rotaviruses and may provide the next generation of rotavirus vaccines. ..
  50. NOVEL CD32 ISOFORMS MODULATE NK CELL FUNCTIONS
    PENELOPE MOREL; Fiscal Year: 2000
    ..abstract_text> ..
  51. MATHEMATICAL MODELING OF IMMUNOLOGICAL REACTIONS
    PENELOPE MOREL; Fiscal Year: 1992
    ....
  52. MATHEMATICAL MODELING OF IMMUNOLOGICAL REACTIONS
    PENELOPE MOREL; Fiscal Year: 1991
    ....
  53. NOVEL CD32 ISOFORMS MODULATE NK CELL FUNCTIONS
    PENELOPE MOREL; Fiscal Year: 1999
    ..abstract_text> ..
  54. Pharmacogenetic Therapy of Pulmonary Hypertension
    Song Li; Fiscal Year: 2003
    ..g. nitric oxide and oxygen), provide an approach to prevent the progressive pathological aspects of PHT and ameliorate return towards more normal pulmonary vascular structure and function. ..
  55. DEVELOPMENT OF ROTAVIRUS DNA VACCINES
    John Herrmann; Fiscal Year: 2003
    ..Immunization with DNA vaccines that express specific rotaviral proteins offers a new approach to vaccination against rotaviruses and may provide the next generation of rotavirus vaccines. ..
  56. Comparative and functional genomics of C. neoformans
    James Kronstad; Fiscal Year: 2003
    ..We will compare the organization and transcriptional regulation of these genes (and a third iron-regulated gene CnURF1) between serotypes. We will also disrupt the genes to examine their role in virulence. ..
  57. LIPID-MEDIATED GENE TRANSFER TO PULMONARY ENDOTHELIUM
    Song Li; Fiscal Year: 2003
    ..These studies will promise to improve the efficiency of gene transfer by LPD and reduce the untoward toxicity. ..
  58. NOVEL CD32 ISOFORMS MODULATE NK CELL FUNCTIONS
    PENELOPE MOREL; Fiscal Year: 2002
    ..abstract_text> ..
  59. In Vivo Study of Synapses in Alzheimer's Disease Models
    Wenbiao Gan; Fiscal Year: 2002
    ....
  60. MATHEMATICAL MODELING OF IMMUNOLOGICAL REACTIONS
    PENELOPE MOREL; Fiscal Year: 2000
    ..abstract_text> ..
  61. Structure/Function Relationship of The Lumican Gene
    WINSTON KAO; Fiscal Year: 2007
    ..g., persistent and excessive inflammation, corneal transparency of injured cornea, improved wound healing of diabetes, etc. ..
  62. CD20 DNA VACCINATION FOR B-CELL LYMPHOMA
    Maria Palomba; Fiscal Year: 2007
    ..Furthermore, she proposes to explore the effect of CD20 immunization in the post-bone marrow transplant settings. Finally, the candidate proposes to carry out a phase I clinical trial of CD20 DNA vaccine in patients with lymphoma. ..
  63. Immunobiological Role of Fertilization Antigen(FA-1)
    RAJESH NAZ; Fiscal Year: 2003
    ....
  64. STRUCTURE/FUNCTION RELATIONSHIP OF LUMICAN
    WINSTON KAO; Fiscal Year: 2000
    ..The resulting homologous recombinant ES cells will be used to prepare knock-in mice, e.g., LumS/S, LumF/F and LumT/T. The phenotypic changes in ECM of such knock- in mice will be examined. ..
  65. Roles of Growth Factors on Corneal Morphogenesis
    WINSTON KAO; Fiscal Year: 2003
    ..g., epithelial debridement, incision, alkali burn. ..
  66. Immunobiological Role of Fertilization Antigen(FA-1)
    RAJESH NAZ; Fiscal Year: 2004
    ....
  67. IMMUNOBIOLOGICAL ROLE OF FERTILIZATION ANTIGEN FA-1
    RAJESH NAZ; Fiscal Year: 1999
    ..Besides enabling the PI to study the structure-function relationship, these studies will help them investigate the potential utility of the human recombinant FA-1 antigen is immunocontraception and immunoinfertility in humans. ..
  68. MATHEMATICAL MODELING OF IMMUNOLOGICAL REACTIONS
    PENELOPE MOREL; Fiscal Year: 1999
    ..abstract_text> ..
  69. Structure/Function Relationship of The Lumican Gene
    Winston W Kao; Fiscal Year: 2010
    ..g., persistent and excessive inflammation, corneal transparency of injured cornea, improved wound healing of diabetes, etc. ..
  70. Comparative and functional genomics of C. neoformans
    James W Kronstad; Fiscal Year: 2010
    ..The research will specifically examine the potential to control infections by targeting the ability of fungal pathogens to acquire the nutrient iron during infection. ..
  71. MATHEMATICAL MODELING OF IMMUNOLOGICAL REACTIONS
    PENELOPE MOREL; Fiscal Year: 2001
    ..abstract_text> ..
  72. Roles of Growth Factors on Corneal Morphogenesis
    Winston W Kao; Fiscal Year: 2010
    ..Data obtained will yield useful information for a better understanding of corneal diseases at molecular and cellular levels in vivo and serve as basis for designing treatment regiments for corneal wound healing. ..
  73. Comparative and functional genomics of C. neoformans
    James Kronstad; Fiscal Year: 2005
    ..We will compare the organization and transcriptional regulation of these genes (and a third iron-regulated gene CnURF1) between serotypes. We will also disrupt the genes to examine their role in virulence. ..
  74. Influenza DNA Vaccine for Biodefense/Pandemic Readiness
    Joel Haynes; Fiscal Year: 2004
    ..These nozzles will be designed to facilitate manufacturing scale-up and to deliver increased DNA/gold payloads to larger skin target areas as a means of increasing the effective vaccine dose. ..
  75. Apoptosis-Resistant Langerhans Cells
    Laura Timares; Fiscal Year: 2002
    ..Moreover, these experiments will lay the foundation toward achieving exogenous control of engineered DC gene expression and, in this case, longevity in vivo. ..
  76. Roles of Growth Factors on Corneal Morphogenesis
    WINSTON KAO; Fiscal Year: 2005
    ..g., epithelial debridement, incision, alkali burn. ..
  77. Regulation of Antitumor Immunity by DC Subsets
    Yukai He; Fiscal Year: 2006
    ..The institute is committed to fully support the candidate by providing access to all of the resources. Together, the candidate, research proposal, and the environment provide a strong application for this award. ..
  78. DEVELOPMENT OF ROTAVIRUS DNA VACCINES
    John Herrmann; Fiscal Year: 2002
    ..Immunization with DNA vaccines that express specific rotaviral proteins offers a new approach to vaccination against rotaviruses and may provide the next generation of rotavirus vaccines. ..
  79. Immunobiological Role of Fertilization Antigen(FA-1)
    RAJESH NAZ; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  80. Immunobiological Role of Fertilization Antigen(FA-1)
    RAJESH NAZ; Fiscal Year: 2005
    ....
  81. Roles of Growth Factors on Corneal Morphogenesis
    WINSTON KAO; Fiscal Year: 2002
    ..g., epithelial debridement, incision, alkali burn. ..
  82. Comparative and functional genomics of C. neoformans
    James Kronstad; Fiscal Year: 2007
    ..We will compare the organization and transcriptional regulation of these genes (and a third iron-regulated gene CnURF1) between serotypes. We will also disrupt the genes to examine their role in virulence. ..
  83. Mice Overexpressing rtTA and Cre in Corneal Epithelium
    WINSTON KAO; Fiscal Year: 2004
    ..These mouse lines will be useful in preparing mouse lines for studies of altered genetic functions from the corneal epithelium-specific gene ablation and overexpression of reporter genes. ..
  84. LIPID-MEDIATED GENE TRANSFER TO PULMONARY ENDOTHELIUM
    Song Li; Fiscal Year: 2002
    ..These studies will promise to improve the efficiency of gene transfer by LPD and reduce the untoward toxicity. ..
  85. Immune response to Pneumocystis in simian model of AIDS
    Karen Norris; Fiscal Year: 2005
    ....
  86. COMPLEMENT REGULATION IN TRYPANOSOMA CRUZI
    Karen Norris; Fiscal Year: 2002
    ..The successful completion of these aims will provide the first functional and immunological evaluation of a critical virulence factor of T. cruzi and provide information and reagents for the use of CRP as a vaccine candidate. ..
  87. COMPLEMENT REGULATION IN TRYPANOSOMA CRUZI
    Karen Norris; Fiscal Year: 2001
    ..The successful completion of these aims will provide the first functional and immunological evaluation of a critical virulence factor of T. cruzi and provide information and reagents for the use of CRP as a vaccine candidate. ..
  88. COMPLEMENT REGULATION IN TRYPANOSOMA CRUZI
    Karen Norris; Fiscal Year: 1999
    ..Studies such as these, which define the molecular basis of an immune evasion mechanism of the parasites, will identify specific targets for the eventual development of immune intervention strategies. ..
  89. Immune response to Pneumocystis in simian model of AIDS
    Karen Norris; Fiscal Year: 2007
    ....
  90. COMPLEMENT REGULATION IN TRYPANOSOMA CRUZI
    Karen Norris; Fiscal Year: 2004
    ..The successful completion of these aims will provide the first functional and immunological evaluation of a critical virulence factor of T. cruzi and provide information and reagents for the use of CRP as a vaccine candidate. ..
  91. Pharmacogenetic Therapy of Pulmonary Hypertension
    Song Li; Fiscal Year: 2006
    ..g. nitric oxide and oxygen), provide an approach to prevent the progressive pathological aspects of PHT and ameliorate return towards more normal pulmonary vascular structure and function. ..
  92. Immune response to Pneumocystis in simian model of AIDS
    Karen Norris; Fiscal Year: 2008
    ....