bacterial typing techniques

Summary

Summary: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.

Top Publications

  1. ncbi Guidelines for the validation and application of typing methods for use in bacterial epidemiology
    A van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:1-46. 2007
  2. pmc Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus
    M C Enright
    Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, Oxford University, Oxford OX1 3FY
    J Clin Microbiol 38:1008-15. 2000
  3. pmc eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data
    Edward J Feil
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom
    J Bacteriol 186:1518-30. 2004
  4. pmc Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis
    Philip Supply
    INSERM U629, Institut Pasteur de Lille, 1, Rue du Prof Calmette, F 59019 Lille cedex, France
    J Clin Microbiol 44:4498-510. 2006
  5. pmc Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions
    Yoko Kondo
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 51:264-74. 2007
  6. pmc Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: a molecular-epidemiological analysis
    Hajo Grundmann
    National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    PLoS Med 7:e1000215. 2010
  7. ncbi An epidemic, toxin gene-variant strain of Clostridium difficile
    L Clifford McDonald
    Epidemiology and Laboratory Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 353:2433-41. 2005
  8. pmc Genotyping of genetically monomorphic bacteria: DNA sequencing in Mycobacterium tuberculosis highlights the limitations of current methodologies
    Iñaki Comas
    Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, UK
    PLoS ONE 4:e7815. 2009
  9. pmc Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates
    Rekha Pai
    Respiratory Diseases Branch, Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Clin Microbiol 44:124-31. 2006
  10. pmc Extended-spectrum beta-lactamases: a clinical update
    David L Paterson
    Infectious Disease Division, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Clin Microbiol Rev 18:657-86. 2005

Research Grants

  1. GOLD QUENCHED MOLECULAR BEACONS
    Richard Powell; Fiscal Year: 2003
  2. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
  3. Intrabody Therapy in Drosophila for Huntington's Disease
    WILLIAM WOLFGANG; Fiscal Year: 2006
  4. Forensic Detection of Salmonella Resistance and Subtype
    Lee Harrison; Fiscal Year: 2005
  5. New Pathogens for Childhood Caries
    Anne Tanner; Fiscal Year: 2008
  6. Fracture Characteristics of Dental Materials
    Luke Hanley; Fiscal Year: 2008
  7. GEOGRAPHIC VARIABILITY IN EMERGENCY DEPARTMENT USE FOR PEDIATRIC ASTHMA
    Jon Mark Hirshon; Fiscal Year: 2007
  8. Diarrhea in Baltimore: Surveillance & Pathogen Detection
    Jon Mark Hirshon; Fiscal Year: 2006
  9. Therapeutic vaccine against tuberculosis
    Lee Riley; Fiscal Year: 2006
  10. ANTIOXIDANT GENES OF MYCOBACERIUM TUBERCULOSIS
    Lee Riley; Fiscal Year: 2001

Detail Information

Publications294 found, 100 shown here

  1. ncbi Guidelines for the validation and application of typing methods for use in bacterial epidemiology
    A van Belkum
    Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands
    Clin Microbiol Infect 13:1-46. 2007
    ..A strategic effort on the part of all workers in the field is thus mandatory to combat emerging infectious diseases, as is financial support from national and international granting bodies and health authorities...
  2. pmc Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus
    M C Enright
    Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, Oxford University, Oxford OX1 3FY
    J Clin Microbiol 38:1008-15. 2000
    ..MLST provides an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the Internet...
  3. pmc eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data
    Edward J Feil
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom
    J Bacteriol 186:1518-30. 2004
    ..pneumoniae and Staphylococcus aureus MLST databases, and the more complicated clonal complexes observed for Campylobacter jejuni and Neisseria meningitidis...
  4. pmc Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis
    Philip Supply
    INSERM U629, Institut Pasteur de Lille, 1, Rue du Prof Calmette, F 59019 Lille cedex, France
    J Clin Microbiol 44:4498-510. 2006
    ..This 15-locus system is therefore proposed as the new standard for routine epidemiological discrimination of M. tuberculosis isolates and the 24-locus system as a high-resolution tool for phylogenetic studies...
  5. pmc Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions
    Yoko Kondo
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 51:264-74. 2007
    ..The SCCmec type assignments were identical to those made with a PCR system that uses numerous primer pairs to identify genes or gene alleles. Our system of six M-PCRs is thus a convenient and reliable method for typing SCCmec elements...
  6. pmc Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: a molecular-epidemiological analysis
    Hajo Grundmann
    National Institute for Public Health and the Environment, Bilthoven, The Netherlands
    PLoS Med 7:e1000215. 2010
    ..We aimed to map the geographic distribution of the dominant clones that cause invasive infections in Europe...
  7. ncbi An epidemic, toxin gene-variant strain of Clostridium difficile
    L Clifford McDonald
    Epidemiology and Laboratory Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 353:2433-41. 2005
    ..difficile with increased virulence, resistance, or both...
  8. pmc Genotyping of genetically monomorphic bacteria: DNA sequencing in Mycobacterium tuberculosis highlights the limitations of current methodologies
    Iñaki Comas
    Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, UK
    PLoS ONE 4:e7815. 2009
    ..Our findings have implications for strain typing in other genetically monomorphic bacteria...
  9. pmc Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates
    Rekha Pai
    Respiratory Diseases Branch, Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Clin Microbiol 44:124-31. 2006
    ....
  10. pmc Extended-spectrum beta-lactamases: a clinical update
    David L Paterson
    Infectious Disease Division, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Clin Microbiol Rev 18:657-86. 2005
    ..ESBLs represent an impressive example of the ability of gram-negative bacteria to develop new antibiotic resistance mechanisms in the face of the introduction of new antimicrobial agents...
  11. pmc Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms
    M C Maiden
    Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, Oxford OX1 3PS, United Kingdom
    Proc Natl Acad Sci U S A 95:3140-5. 1998
    ....
  12. ncbi Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers
    Heiman F L Wertheim
    Erasmus MC, University Medical Centre, Rotterdam, Department of Medical Microbiology and Infectious Diseases, Dr Molewaterplein 40, 3015 GD Rotterdam, Netherlands
    Lancet 364:703-5. 2004
    ..006). S aureus nasal carriers and non-carriers differ significantly in risk and outcome of nosocomial S aureus bacteraemia. Genotyping revealed that 80% of strains causing bacteraemia in carriers were endogenous...
  13. pmc Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases
    Jill E Clarridge
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Clin Microbiol Rev 17:840-62, table of contents. 2004
    ....
  14. pmc How clonal is Staphylococcus aureus?
    Edward J Feil
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, United Kingdom
    J Bacteriol 185:3307-16. 2003
    ....
  15. pmc Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolates
    Caroline Allix-Béguec
    Genoscreen, 1, rue du Professeur Calmette, Lille 59019 Cedex, France
    J Clin Microbiol 46:2692-9. 2008
    ..The MIRU-VNTRplus database is a powerful tool for high-resolution clonal identification and has little equivalent in terms of functionalities among the bacterial genotyping databases available so far...
  16. pmc Methicillin-resistant Staphylococcus aureus (MRSA) strain ST398 is present in midwestern U.S. swine and swine workers
    Tara C Smith
    Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA, USA
    PLoS ONE 4:e4258. 2009
    ..However, no studies to date have investigated carriage of MRSA among swine and swine farmers in the United States (U.S.)...
  17. pmc Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii
    Sergio G Bartual
    Division de Microbiologia and Evolutionary Genomics Group, Campus de San Juan, Universidad Miguel Hernandez, San Juan de Alicante, Spain
    J Clin Microbiol 43:4382-90. 2005
    ..The MLST scheme provides a high level of resolution and an excellent tool for studying the population structure and long-term epidemiology of A. baumannii...
  18. pmc Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates
    Laure Diancourt
    Unité Biodiversité des Bactéries Pathogènes Emergentes U389 INSERM, Institut Pasteur, 25 28 Rue du Dr Roux, 75724 Paris, Cedex 15, France
    J Clin Microbiol 43:4178-82. 2005
    ..Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates...
  19. pmc Multilocus sequence typing system for Campylobacter jejuni
    K E Dingle
    Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, Oxford OX1 3FY, United Kingdom
    J Clin Microbiol 39:14-23. 2001
    ..Application of this approach to further isolate collections will enable an integrated global picture of C. jejuni epidemiology to be established and will permit more detailed studies of the population genetics of this organism...
  20. pmc Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spre
    Stephen Murchan
    Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 41:1574-85. 2003
    ..This highlights the need for closer international collaboration to monitor the spread of current epidemic strains as well as the emergence of new ones...
  21. ncbi Infection and colonization with methicillin resistant Staphylococcus aureus ST398 versus other MRSA in an area with a high density of pig farms
    M W H Wulf
    PAMM Laboratory for Medical Microbiology, de Run 6250, Veldhoven, The Netherlands
    Eur J Clin Microbiol Infect Dis 31:61-5. 2012
    ..ST398 isolates were more often multi-resistant than isolates of other spa-types. The emergence of MRSA ST398 led to an increase in both MRSA carriers and MRSA infections...
  22. pmc Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units
    P Supply
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, F 59019 Lille cedex, France
    J Clin Microbiol 39:3563-71. 2001
    ..tuberculosis MIRU-VNTR genotypes via the Internet. This opens the way for global epidemiological surveillance of tuberculosis and should lead to novel insights into the evolutionary and population genetics of this major pathogen...
  23. ncbi Possible underlying mechanisms for successful emergence of the Mycobacterium tuberculosis Beijing genotype strains
    Ida Parwati
    Department of Clinical Pathology, Medical Faculty, Padjadjaran University, Dr Hasan Sadikin Hospital, Bandung, Indonesia
    Lancet Infect Dis 10:103-11. 2010
    ..More research is needed to further unravel the mechanisms underlying the emergence of M tuberculosis Beijing genotype strains, and examine the implications for future control strategies...
  24. pmc Global phylogeny of Mycobacterium tuberculosis based on single nucleotide polymorphism (SNP) analysis: insights into tuberculosis evolution, phylogenetic accuracy of other DNA fingerprinting systems, and recommendations for a minimal standard SNP set
    Ingrid Filliol
    Division of Infectious Disease, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave, MSB A920C, Newark, NJ 07103
    J Bacteriol 188:759-72. 2006
    ..tuberculosis...
  25. pmc Ribosomal multilocus sequence typing: universal characterization of bacteria from domain to strain
    Keith A Jolley
    Department of Zoology, University of Oxford, Oxford, UK
    Microbiology 158:1005-15. 2012
    ..This approach is applicable to the other domains of life, potentially providing a rational and universal approach to the classification of life that is based on one of its fundamental features, the translation mechanism...
  26. ncbi Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology
    Erko Stackebrandt
    DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany
    Int J Syst Evol Microbiol 52:1043-7. 2002
    ..The committee made various recommendations regarding the species definition in the light of developments in methodologies available to systematists...
  27. ncbi Prevalence of methicillin-resistant Staphylococcus aureus among veterinarians: an international study
    M W H Wulf
    Department of Medical Microbiology, Nijmegen University Centre for Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Microbiol Infect 14:29-34. 2008
    ....
  28. pmc Based Upon Repeat Pattern (BURP): an algorithm to characterize the long-term evolution of Staphylococcus aureus populations based on spa polymorphisms
    Alexander Mellmann
    Institute for Hygiene, University Hospital Munster, Munster, Germany
    BMC Microbiol 7:98. 2007
    ..BURP was subsequently evaluated with a strain collection reflecting the breadth of diversity of S. aureus (JCM 2002; 40:4544)...
  29. pmc Diversity of SCCmec elements in methicillin-resistant coagulase-negative staphylococci clinical isolates
    Zhiyong Zong
    Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China
    PLoS ONE 6:e20191. 2011
    ..Characterization of SCCmec in MR-CoNS can generate useful information on the mobilization and evolution of this element...
  30. pmc Evaluation of a two-step approach for large-scale, prospective genotyping of Mycobacterium tuberculosis isolates in the United States
    Lauren S Cowan
    Division of TB Elimination, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 43:688-95. 2005
    ..IS6110 fingerprinting can then be used as a secondary typing method to type the clustered isolates when additional discrimination is needed...
  31. ncbi A novel multilocus sequence typing scheme for the opportunistic pathogen Propionibacterium acnes and characterization of type I cell surface-associated antigens
    Andrew McDowell
    Centre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, Queen s University, 97 Lisburn Road, Belfast BT9 7BL, UK
    Microbiology 157:1990-2003. 2011
    ..The MLST scheme and database described in this study should provide a valuable platform for future epidemiological and evolutionary studies of P. acnes...
  32. ncbi Hyper-recombination, diversity, and antibiotic resistance in pneumococcus
    William Paul Hanage
    Department of Infectious Disease Epidemiology, Imperial College London, Norfolk Place, London W2 1PG, UK
    Science 324:1454-7. 2009
    ..This could have consequences for the reemergence of drug resistance after pneumococcal vaccination and also for our understanding of diversification and speciation in recombinogenic bacteria...
  33. pmc Improved multilocus sequence typing scheme for Staphylococcus epidermidis
    Jonathan C Thomas
    Department of Infectious Disease Epidemiology, St Mary s Hospital Campus, Imperial College London, London W2 1PG, United Kingdom
    J Clin Microbiol 45:616-9. 2007
    ..This improved scheme gave 31 sequence types (STs) and 5 clonal complexes (CCs), whereas the other schemes delineate 16 to 24 STs and 1 to 3 CCs...
  34. ncbi The evolution of Staphylococcus aureus
    Ruud H Deurenberg
    Department of Medical Microbiology, University Hospital Maastricht, P Debyelaan 25, 6229 HX Maastricht, The Netherlands
    Infect Genet Evol 8:747-63. 2008
    ..This review describes the latest observations about the structure of SCCmec, the techniques used to study the molecular epidemiology and evolution of S. aureus as well as some challenges that researchers face in the future...
  35. ncbi Evaluation of Brucella MLVA typing for human brucellosis
    Sascha Al Dahouk
    Bundeswehr Institute of Microbiology, Department of Bacteriology, Neuherbergstr 11, D 80937 Munich, Germany
    J Microbiol Methods 69:137-45. 2007
    ..MLVA can significantly contribute to epidemiological trace-back analysis of Brucella infections and may advance surveillance and control of human brucellosis...
  36. pmc Estimating recombinational parameters in Streptococcus pneumoniae from multilocus sequence typing data
    E J Feil
    Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, United Kingdom
    Genetics 154:1439-50. 2000
    ..We also demonstrate how to estimate the average length of recombinational replacements from MLST data...
  37. pmc High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology
    E Mazars
    , , U447, Institut Pasteur de Lille, rue du Prof. Calmette, F-59019 Lille Cedex, France
    Proc Natl Acad Sci U S A 98:1901-6. 2001
    ..This typing method opens the way to the construction of digital global databases for molecular epidemiology studies of M. tuberculosis...
  38. ncbi Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: 'SCCmec IV multiplex'
    Catarina Milheiriço
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    J Antimicrob Chemother 60:42-8. 2007
    ..To develop and validate a new multiplex PCR strategy for subtyping SCCmec type IV methicillin-resistant Staphylococcus aureus (MRSA) strains-SCCmec IV multiplex PCR...
  39. ncbi A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease
    M C Enright
    Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, UK
    Microbiology 144:3049-60. 1998
    ..A Web site for the molecular characterization of pneumococci by MLST is available (http ://mlst.zoo.ox.ac.uk)...
  40. ncbi Displaying the relatedness among isolates of bacterial species -- the eBURST approach
    Brian G Spratt
    Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, St Mary s Hospital, Old Medical School Building, London W2 1PG, UK
    FEMS Microbiol Lett 241:129-34. 2004
    ..We discuss the advantages of this approach and describe its use to explore patterns of descent within clonal complexes identified using multilocus sequence typing...
  41. pmc Coxiella burnetii in humans and ticks in rural Senegal
    Oleg Mediannikov
    URMITE, UMR IRD 198 CNRS 6236, Mediterranean University, Medical Faculty, Marseille, France
    PLoS Negl Trop Dis 4:e654. 2010
    ..Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Epidemiologically, animals are considered reservoirs and humans incidental hosts...
  42. pmc Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis
    Mara Cardoso Oelemann
    INSERM, U629, Institut Pasteur de Lille, Lille, France
    J Clin Microbiol 45:691-7. 2007
    ..tuberculosis transmission...
  43. pmc spa Typing of Staphylococcus aureus as a frontline tool in epidemiological typing
    B Strommenger
    Robert Koch Institute, Wernigerode Branch, Burgstr 37, D 38855 Wernigerode, Germany
    J Clin Microbiol 46:574-81. 2008
    ..They should be selected by dependence on the clonal lineage indicated by spa typing and subsequent BURP analysis as well as on the basis of the particular question to be addressed...
  44. ncbi Resolving lineage assignation on Mycobacterium tuberculosis clinical isolates classified by spoligotyping with a new high-throughput 3R SNPs based method
    Edgar Abadia
    Institute of Genetics and Microbiology, UMR8621, IGEPE Team, UniverSud, CNRS Université Paris Sud 11, France
    Infect Genet Evol 10:1066-74. 2010
    ..It also allows evaluation of spoligotype-signature significance towards a more comprehensive understanding of the evolutionary mechanisms of the clustered regularly interspaced short palindromic repeat (CRISPR) locus in MTC...
  45. pmc Multilocus sequence typing of Clostridium difficile
    David Griffiths
    Nuffield Department of Clinical Medicine, Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
    J Clin Microbiol 48:770-8. 2010
    ..difficile in total stool DNA extracts without isolate culture. The direct (nonculture) MLST approach may prove useful as a rapid genotyping method, potentially benefiting individual patients and informing hospital infection control...
  46. pmc Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs on German farms and import of livestock-related MRSA into hospitals
    R Kock
    Institute of Hygiene, University Hospital Munster, Robert Koch Str 41, 48149, Munster, Germany
    Eur J Clin Microbiol Infect Dis 28:1375-82. 2009
    ..Our results indicate that livestock represents a relevant reservoir for the import of MRSA into regional German hospitals...
  47. pmc Genomic diversity among drug sensitive and multidrug resistant isolates of Mycobacterium tuberculosis with identical DNA fingerprints
    Stefan Niemann
    Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
    PLoS ONE 4:e7407. 2009
    ..According to the standard view, isolates exhibiting the same fingerprinting pattern are considered direct progeny of the same bacterial clone, and most likely reflect ongoing transmission or disease relapse within individual patients...
  48. pmc Three-year population-based evaluation of standardized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing of Mycobacterium tuberculosis
    Caroline Allix-Béguec
    Molecular Mechanisms of Bacterial Pathogenesis, INSERM U629, Institut de Biologie de Lille Institut Pasteur de Lille, 1 rue du Professeur Calmette, F 59021 Lille cedex, France
    J Clin Microbiol 46:1398-406. 2008
    ..g., large sequence polymorphisms. Our results reinforce the proposal of standardized MIRU-VNTR typing as a new reference genotyping method for the epidemiological and phylogenetic screening of M. tuberculosis strains...
  49. ncbi Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study
    Vincent Perreten
    Institute of Veterinary Bacteriology, University of Berne, Berne, Switzerland
    J Antimicrob Chemother 65:1145-54. 2010
    ..The aim of this study was to determine the phenotypic and genotypic resistance profiles of methicillin-resistant Staphylococcus pseudintermedius (MRSP) and to examine the clonal distribution in Europe and North America...
  50. pmc Host association of Campylobacter genotypes transcends geographic variation
    Samuel K Sheppard
    Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom
    Appl Environ Microbiol 76:5269-77. 2010
    ..These findings are consistent with local and international transmission of host-associated lineages among food animal species and provide a foundation for further improvements in genetic attribution...
  51. ncbi Are host genetics the predominant determinant of persistent nasal Staphylococcus aureus carriage in humans?
    Raymond Ruimy
    EA3964 Université Paris Diderot and Centre National de Référence, Résistance bactérienne dans les flores commensales, Hopital Bichat Claude Bernard, AP HP, Paris, France
    J Infect Dis 202:924-34. 2010
    ..Therefore, we concomitantly assessed the epidemiological, microbiological, and human-genetic carriage-related factors in a nearly closed population...
  52. ncbi Global emm type distribution of group A streptococci: systematic review and implications for vaccine development
    Andrew C Steer
    Centre for International Child Health, University of Melbourne, Australia
    Lancet Infect Dis 9:611-6. 2009
    ....
  53. ncbi Trends in production of extended-spectrum beta-lactamases among Enterobacteriaceae of clinical interest: results of a nationwide survey in Belgian hospitals
    H Rodriguez-Villalobos
    Laboratoire de Microbiologie, Hopital Universitaire Erasme, Universite Libre de Bruxelles, B 1070 Brussels, Belgium
    J Antimicrob Chemother 66:37-47. 2011
    ..to assess the frequency and diversity of extended-spectrum β-lactamases (ESBLs) in Enterobacteriaceae isolates in Belgium...
  54. pmc Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry
    Bernard La Scola
    Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e8041. 2009
    ..In order to speed up the identification process, in the present work we attempted bacterial identification directly from blood culture bottles detected positive by the automate...
  55. ncbi Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition
    Stefan Monecke
    Institute for Medical Microbiology and Hygiene, Faculty of Medicine Carl Gustav Carus, Technical University of Dresden, Dresden, Germany
    FEMS Immunol Med Microbiol 53:237-51. 2008
    ..Additionally, there are some isolated lineages, such as ST75, ST93, or ST152. These strains produce aberrant hybridization profiles, indicating that only a part of the gene pool of S. aureus has been investigated yet...
  56. ncbi Molecular epidemiologic typing systems of bacterial pathogens: current issues and perspectives
    M J Struelens
    Service de Microbiologie, Hopital Erasme, Bruxelles, Belgium
    Mem Inst Oswaldo Cruz 93:581-5. 1998
    ..Accurate epidemiological interpretation of data obtained with molecular typing systems still requires additional research on the evolution rate of polymorphic loci in bacterial pathogens...
  57. pmc Nomenclature of major antimicrobial-resistant clones of Streptococcus pneumoniae defined by the pneumococcal molecular epidemiology network
    L McGee
    MRC SAIMR Wits Pneumococcal Diseases Research Unit, Johannesburg, South Africa
    J Clin Microbiol 39:2565-71. 2001
    ..This network can serve as a prototype for the collaboration of scientists in identifying clones of important human pathogens and as a model for the development of other networks...
  58. pmc Pseudomonas aeruginosa population structure revisited
    Jean Paul Pirnay
    Laboratory for Molecular and Cellular Technology, Burn Centre, Queen Astrid Military Hospital, Brussel, Belgium
    PLoS ONE 4:e7740. 2009
    ..aeruginosa "core lineage" and typically exhibited the exoS(+)/exoU(-) genotype and group B oprL and oprD alleles. This is to our knowledge the first report of an MST analysis conducted on a polyphasic data set...
  59. ncbi Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing
    P Bidet
    Laboratoire de Bacteriologie, Centre Hospitalo Universitaire Saint Antoine, Assistance Publique Hopitaux de Paris, France
    FEMS Microbiol Lett 175:261-6. 1999
    ..This typing method has evidenced major qualities such as easiness, rapidity and reproducibility. However, its discriminatory power has to be evaluated to validate its importance as a typing tool for C. difficile...
  60. ncbi DNA microarray-based genotyping of methicillin-resistant Staphylococcus aureus strains from Eastern Saxony
    S Monecke
    Institute for Medical Microbiology and Hygiene, Faculty of Medicine Carl Gustav Carus, Technical University of Dresden, Dresden, Germany
    Clin Microbiol Infect 14:534-45. 2008
    ..Detection of such variants can sometimes allow epidemic strains to be resolved beyond spa types to a hospital-specific level, which is of significant value for epidemiological purposes...
  61. pmc Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei
    Horng Yunn Dou
    Division of Clinical Research, National Health Research Institutes, Zhunan, Taiwan, Republic of China
    BMC Infect Dis 8:170. 2008
    ..Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping, were applied after culture...
  62. pmc Development of a multilocus sequence typing scheme for the opportunistic pathogen Pseudomonas aeruginosa
    Barry Curran
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Clin Microbiol 42:5644-9. 2004
    ..aeruginosa, the lack of a linkage between toxA type and serotype is not surprising and reveals the strength of the MLST approach for obtaining a better understanding of the epidemiology of P. aeruginosa...
  63. pmc PCR-based quantitation and clonal diversity of the current prevalent invasive serogroup 6 pneumococcal serotype, 6C, in the United States in 1999 and 2006 to 2007
    Maria Da Gloria Carvalho
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 47:554-9. 2009
    ..Fifteen 6C isolates shared one of four different MLST types with 6C-negative CS6As. MLST results suggest 6C strains arose from independent recombination events involving only serotype 6A and 6C parental strains...
  64. pmc Tracing the source of campylobacteriosis
    Daniel J Wilson
    Department of Maths and Statistics, Lancaster University, Lancaster, United Kingdom
    PLoS Genet 4:e1000203. 2008
    ..Our results imply that the primary transmission route is through the food chain, and suggest that incidence could be dramatically reduced by enhanced on-farm biosecurity or preventing food-borne transmission...
  65. pmc spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation
    Larry Koreen
    Public Health Research Institute, International Center for Public Health, Graduate School of Biomedical Sciences and New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Clin Microbiol 42:792-9. 2004
    ..This study showed that genetic analysis of the repeat region of protein A comprehensively characterizes both micro- and macrovariation in the primarily clonal population structure of S. aureus...
  66. pmc Emergence of a community-associated methicillin-resistant Staphylococcus aureus strain with a unique resistance profile in Southwest Nigeria
    B Ghebremedhin
    Otto von Guericke University Clinic, Medical Microbiology, Magdeburg, Germany
    J Clin Microbiol 47:2975-80. 2009
    ..Urban regions with populations of lower socioeconomic status and evidence of overcrowding appear to be at high risk for the emergence of this clone...
  67. pmc Comparison of seven techniques for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat an
    George Killgore
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Clin Microbiol 46:431-7. 2008
    ..All techniques appear to be capable of detecting outbreak strains, but only REA and MLVA showed sufficient discrimination to distinguish strains from different outbreaks...
  68. ncbi Bacterial strain typing in the genomic era
    Wenjun Li
    Unité des Rickettsies et pathogènes émergents, CNRS UMR 6236, IRD 198, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    FEMS Microbiol Rev 33:892-916. 2009
    ....
  69. pmc Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing
    B Strommenger
    Wernigerode Branch, Robert Koch Institute, Burgstr 37, D 38855 Wernigerode, and Department of Periodontology, University Hospital Munster, Germany
    J Clin Microbiol 44:2533-40. 2006
    ..aureus epidemiology, especially because of ease of use and the advantages of unambiguous sequence analysis as well as reproducibility and exchange of typing data...
  70. pmc Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing
    Wei Gang Qiu
    Department of Biological Sciences, Hunter College of the City University of New York, 695 Park Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:14150-5. 2004
    ..Frequent recombination implies a potential for rapid adaptive evolution and a possible polygenic basis of B. burgdorferi pathogenicity...
  71. pmc Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains
    Françoise Jaureguy
    INSERM UMR570, Faculte de Medecine, Universite Paris Descartes, Paris, France
    BMC Genomics 9:560. 2008
    ..A subset representative of the clonal diversity was subjected to comparative genomic hybridization (CGH) using 2,324 amplicons from the flexible gene pool of E. coli...
  72. ncbi Livestock associated methicillin-resistant Staphylococcus aureus (LaMRSA) isolated from lesions of pigs at necropsy in northwest Germany between 2004 and 2007
    D Meemken
    Field Station for Epidemiology, University of Veterinary Medicine Hannover, Foundation, Bakum, Germany
    Zoonoses Public Health 57:e143-8. 2010
    ..We assume that MRSA in pigs may have occurred earlier than 2004 and might be not really 'emerging', but rather have been overlooked until recently. The potentially causative role of the MRSA in the lesions warrants further investigation...
  73. pmc Molecular evidence of cholera outbreak caused by a toxigenic Vibrio cholerae O1 El tor variant strain in Kelantan, Malaysia
    Geik Yong Ang
    Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu 16150, Malaysia
    J Clin Microbiol 48:3963-9. 2010
    ..cholerae O1 El Tor variant has invaded Malaysia, highlighting the need for continuous monitoring to facilitate early interventions against any potential epidemic by this biotype...
  74. ncbi Evaluation of 24 locus MIRU-VNTR genotyping of Mycobacterium tuberculosis isolates in Canada
    Sara Christianson
    National Microbiology Laboratory, 1015 Arlington Avenue, Winnipeg, Manitoba R3E 3R2, Canada
    Tuberculosis (Edinb) 90:31-8. 2010
    ..For long-term surveillance of MTBC within Canada, the use of 24 MIRU-VNTR loci will provide rapid, highly discriminatory molecular epidemiology information...
  75. ncbi Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry
    E Nagy
    Institute of Clinical Microbiology, University of Szeged, H 6701 Szeged, Hungary
    Clin Microbiol Infect 15:796-802. 2009
    ..The discriminating power and identification accuracy of MALDI-TOF MS proved to be superior to that of biochemical testing for Bacteroides thetaiotaomicron, Bacteroides ovatus and Bacteroides uniformis...
  76. ncbi Changes in pneumococcal serotypes and antibiotypes carried by vaccinated and unvaccinated day-care centre attendees in Portugal, a country with widespread use of the seven-valent pneumococcal conjugate vaccine
    R Sá-Leão
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Clin Microbiol Infect 15:1002-7. 2009
    ..Rates of antibiotic resistance remained unchanged due to a balance between reduction in VT and an increase in antimicrobial-resistant NVT...
  77. pmc Bacterial flora-typing with targeted, chip-based Pyrosequencing
    Andreas Sundquist
    Department of Computer Science, Stanford University, Stanford, CA 94305, USA
    BMC Microbiol 7:108. 2007
    ..In particular, classifying the microbial content of samples by sequencing the < 1,600 bp 16S rRNA gene will be affected by such limitations...
  78. pmc Comparison of two multilocus variable-number tandem-repeat methods and pulsed-field gel electrophoresis for differentiating highly clonal methicillin-resistant Staphylococcus aureus isolates
    A Holmes
    Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom
    J Clin Microbiol 48:3600-7. 2010
    ..However, due to its greater discriminatory power, MLVF would be the most suitable alternative to PFGE for hospital outbreak investigations...
  79. ncbi Staphylococcus aureus nasal carriage, virulence traits, antibiotic resistance mechanisms, and genetic lineages in healthy humans in Spain, with detection of CC398 and CC97 strains
    Carmen Lozano
    Area Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006 Logrono, Spain
    Int J Med Microbiol 301:500-5. 2011
    ..Interestingly, MSSA CC398 and CC97 isolates were detected. These clonal complexes are associated with food-producing animals...
  80. ncbi Molecular methods for Mycobacterium tuberculosis strain typing: a users guide
    E Kanduma
    Clinical Laboratory, Kilimanjaro Christian Medical College, PO Box 3010, Moshi, Tanzania
    J Appl Microbiol 94:781-91. 2003
    ..If matches are found, then further confirmation of identity can be achieved using IS6110 RFLP or PGRS if the strains prove to have a low IS6110 copy number...
  81. pmc Detection of Streptococcus pneumoniae strain cocolonization in the nasopharynx
    Silvio D Brugger
    Institute for Infectious Diseases, University of Bern, Friedbuhlstrasse 51, CH 3010 Bern, Switzerland
    J Clin Microbiol 47:1750-6. 2009
    ..025). This new technique allows for the rapid and economical study of pneumococcal cocolonization in nasopharyngeal swabs. It will be valuable for the surveillance of S. pneumoniae epidemiology under vaccine selection pressure...
  82. ncbi Ecotypes of the Mycobacterium tuberculosis complex
    Noel H Smith
    TB Research Group, Veterinary Laboratories Agency, VLA, Addlestone, Surrey KT15 3NB, UK
    J Theor Biol 239:220-5. 2006
    ..Finally, we discuss the application of the ecotype concept to this series of clades and suggest that the M. tuberculosis complex may best be described as a series of host-adapted ecotypes...
  83. ncbi Assigning Escherichia coli strains to phylogenetic groups: multi-locus sequence typing versus the PCR triplex method
    David M Gordon
    School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia
    Environ Microbiol 10:2484-96. 2008
    ..Strains failing to yield any PCR products using the Clermont method are seldom members of phylo-group A and strains with such a genotype should not be assigned to a phylo-group...
  84. ncbi Linkage disequilibrium between minisatellite loci supports clonal evolution of Mycobacterium tuberculosis in a high tuberculosis incidence area
    Philip Supply
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, Cedex, France
    Mol Microbiol 47:529-38. 2003
    ..This linkage disequilibrium was also evident when the MIRU-VNTR types were compared with the IS6110 restriction fragment length polymorphism types. These results support a predominant clonal evolution of M. tuberculosis...
  85. pmc Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus
    Kunyan Zhang
    Centre for Antimicrobial Resistance, Calgary Health Region Calgary Laboratory Services University of Calgary, Calgary, Alberta, Canada T2L 2K8
    J Clin Microbiol 43:5026-33. 2005
    ..This novel assay offers a rapid, simple, and feasible method for SCCmec typing of MRSA, and may serve as a useful tool for clinicians and epidemiologists in their efforts to prevent and control infections caused by this organism...
  86. pmc MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
    Marianne Maquart
    INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, F 37380, France
    BMC Microbiol 9:145. 2009
    ..A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used...
  87. pmc Community-associated methicillin-resistant Staphylococcus aureus isolated in Finland in 2004 to 2006
    Mari Kanerva
    National Institute for Health and Welfare, Department of Infectious Disease Epidemiology and Control, Helsinki, Finland
    J Clin Microbiol 47:2655-7. 2009
    ..9/100,000 population) and strain variation increased in comparison to years 1997 to 1999. There were 7 community-associated epidemic and 25 sporadic MRSA strain types. Half of these had Panton-Valentine leukocidin genes...
  88. ncbi Genetic determinants of virulence, antibiogram and altered biotype among the Vibrio cholerae O1 isolates from different cholera outbreaks in India
    A K Goel
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Infect Genet Evol 10:815-9. 2010
    ..In addition, in 2007, a new variant of O1 El Tor strain with further modifications in ctxB of classical biotype is discovered among the Indian isolates...
  89. pmc First multi-locus sequence typing scheme for Arcobacter spp
    William G Miller
    Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA
    BMC Microbiol 9:196. 2009
    ..Here, we describe the first Arcobacter multilocus sequence typing (MLST) method for A. butzleri, A. cryaerophilus, A. skirrowii, A. cibarius and A. thereius...
  90. pmc SNP/RD typing of Mycobacterium tuberculosis Beijing strains reveals local and worldwide disseminated clonal complexes
    Anita C Schürch
    Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment RIVM, Centre for Infectious Disease Control, CIb LIS, pb 22, Bilthoven, The Netherlands
    PLoS ONE 6:e28365. 2011
    ..The SNPs and RDs presented in this study will facilitate future molecular epidemiological and phylogenetic studies on Beijing strains...
  91. ncbi Comparison of genotypes and antibiotic resistance of Campylobacter jejuni isolated from humans and slaughtered chickens in Switzerland
    S Kittl
    Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland
    J Appl Microbiol 110:513-520. 2011
    ..To get an overview of genotypes and antibiotic resistances in Swiss Campylobacter jejuni implicated in human gastroenteritis and to examine the association with isolates from chickens...
  92. ncbi Mutation rates of spoligotypes and variable numbers of tandem repeat loci in Mycobacterium tuberculosis
    Josephine F Reyes
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
    Infect Genet Evol 10:1046-51. 2010
    ..These findings confirm the high level of discrimination observed using multilocus VNTR typing, and suggest that caution should be taken not to underestimate the extent of recent transmission when using this marker...
  93. pmc Sharing of Escherichia coli sequence type ST131 and other multidrug-resistant and Urovirulent E. coli strains among dogs and cats within a household
    James R Johnson
    Infectious Diseases 111F, Veterans Affairs Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    J Clin Microbiol 47:3721-5. 2009
    ..Of the four other colonizing strains identified, two were MDR and two colonized multiple hosts. The ST131 strain uniquely exhibited high resistance and virulence scores...
  94. ncbi Comparative evaluation of new typing schemes for urogenital Chlamydia trachomatis isolates
    Larisa N Ikryannikova
    Research Institute for Physical Chemical Medicine of Ministry of Public Health of the Russian Federation, Moscow, Russia
    FEMS Immunol Med Microbiol 59:188-96. 2010
    ..trachomatis population in Central Russia using MLST. We conclude that the MLST(7) scheme is the best possible choice for global epidemiological purposes, whereas MLST(5) is more appropriate for tracing local outbreaks...
  95. ncbi Rapid and high resolution genotyping of all Escherichia coli serotypes using 10 genomic repeat-containing loci
    Inger Løbersli
    Division of Infectious Diseases Control, Norwegian Institute of Public Health, Lovisenberggata 8, P O Box 4404 Nydalen, N 0403 Oslo, Norway
    J Microbiol Methods 88:134-9. 2012
    ..The GECM7 method was improved by adding three new repeat-loci to a total of ten (GECM10), and a considerable increase in resolution was observed (from 296 to 507 genotypes on the same set of strains)...
  96. pmc Evaluation of new variable-number tandem-repeat systems for typing Mycobacterium tuberculosis with Beijing genotype isolates from Beijing, China
    Wei Wei Jiao
    Public Central Laboratory, Beijing Pediatric Research Institute, Beijing Children s Hospital Affiliated to Capital Medical University, Beijing 100045, China
    J Clin Microbiol 46:1045-9. 2008
    ..tuberculosis isolates in Beijing, while secondary typing of clustered strains by IS6110-RFLP is still required...
  97. ncbi Biotyping of Vibrio cholerae O1: time to redefine the scheme
    Amit Raychoudhuri
    Division of Microbiology, National Institute of Cholera and Enteric Diseases, Kolkata, India
    Indian J Med Res 128:695-8. 2008
    ..A revised biotyping scheme will play a significant role to understand the ecology, epidemiology and nature of infection of V. cholerae O1 strains in future...
  98. pmc Genome-wide single nucleotide polymorphism typing method for identification of Bacillus anthracis species and strains among B. cereus group species
    Makoto Kuroda
    Laboratory of Bacterial Genomics, Pathogen Genomics Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    J Clin Microbiol 48:2821-9. 2010
    ..Subsequent whole-genome sequencing will reveal apparent strain-specific genetic markers for traceability of strains following an anthrax outbreak...
  99. ncbi Identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis
    Andrea T Fessler
    Institute of Farm Animal Genetics, Friedrich Loeffler Institut FLI, Neustadt Mariensee, Germany
    J Antimicrob Chemother 65:1576-82. 2010
    ....
  100. pmc New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh
    G Balakrish Nair
    Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1000, Bangladesh
    J Clin Microbiol 40:3296-9. 2002
    ..Furthermore, the existence of such novel strains may have implications for the development of a cholera vaccine...
  101. ncbi Global serotype distribution among Streptococcus pneumoniae isolates causing otitis media in children: potential implications for pneumococcal conjugate vaccines
    Gail L Rodgers
    Scientific Affairs and Global Medical Affairs, Vaccines, Wyeth Pharmaceuticals Inc, Collegeville, PA 19426, USA
    Vaccine 27:3802-10. 2009
    ....

Research Grants46

  1. GOLD QUENCHED MOLECULAR BEACONS
    Richard Powell; Fiscal Year: 2003
    ..abstract_text> ..
  2. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  3. Intrabody Therapy in Drosophila for Huntington's Disease
    WILLIAM WOLFGANG; Fiscal Year: 2006
    ..If successful this will provide validation of intrabodies as disease therapeutics and impetus to proceed to preclinical testing in vertebrate models of HD. ..
  4. Forensic Detection of Salmonella Resistance and Subtype
    Lee Harrison; Fiscal Year: 2005
    ..enterica and 2) molecular methods for rapid outbreak detection among S. enterica serovars. ..
  5. New Pathogens for Childhood Caries
    Anne Tanner; Fiscal Year: 2008
    ..As a result, it is expected that many of the negative consequences of this disease will be avoided, with attendant reductions in the costs of dental and related medical care. ..
  6. Fracture Characteristics of Dental Materials
    Luke Hanley; Fiscal Year: 2008
    ..abstract_text> ..
  7. GEOGRAPHIC VARIABILITY IN EMERGENCY DEPARTMENT USE FOR PEDIATRIC ASTHMA
    Jon Mark Hirshon; Fiscal Year: 2007
    ..The research has potential nationwide applicability and could lead to innovative strategies to meet the Healthy People 2010 objective 24-3 focused on decreasing ED utilization for asthma. ..
  8. Diarrhea in Baltimore: Surveillance & Pathogen Detection
    Jon Mark Hirshon; Fiscal Year: 2006
    ..Through this work, we expect to not only better define the causes of diarrheal illnesses in the Baltimore Metropolitan area, but to identify other pathogens that contribute to foodborne illnesses. ..
  9. Therapeutic vaccine against tuberculosis
    Lee Riley; Fiscal Year: 2006
    ..Therapeutic vaccines against TB is a major priority in TB control, and we have an opportunity to identify such products by a novel approach using a simple, inexpensive, and reproducible animal model. ..
  10. ANTIOXIDANT GENES OF MYCOBACERIUM TUBERCULOSIS
    Lee Riley; Fiscal Year: 2001
    ....
  11. Epidemiology of an uropathogenic E coli clonal group
    Lee Riley; Fiscal Year: 2003
    ..coli can be spread by contaminated ingestible vehicles, and use the data generated from this project to design long-term studies to better characterize risk factors associated with CgA infection. ..
  12. MODULATION OF CELL SIGNALING BY PSEUDOMONAS EXOENZYME S
    Joan Olson; Fiscal Year: 2003
    ....
  13. INITIAL LESION OF ADULT PERIODONTITIS
    Anne Tanner; Fiscal Year: 2002
    ....
  14. Pneumococcal conjugate vaccines and old adults
    MOON NAHM; Fiscal Year: 2009
    ..This study will enhance the knowledge of pneumococcal antibodies made by old adults and will help us determine if PCVs can be useful in pneumococcal vaccination of old adults, a major goal of our national health care policy. ..
  15. In vivo regulation of M. tuberculosis cell wall lipids
    Lee W Riley; Fiscal Year: 2010
    ..tuberculosis and granulomas may contribute to our understanding of the mechanism of persistence of M. tuberculosis that could lead to new tests to differentiate active TB from latent TB infection. ..
  16. Novel Chemical Analyses of the Biofilm-Biomaterial Interface
    Luke Hanley; Fiscal Year: 2010
    ..Specific Aim 2: Probe antibiotic resistance in S. epidermidis biofilms. Specific Aim 3: Probe RIP inhibition of S. epidermidis biofilm growth. Specific Aim 4: Probe chitosan inhibition of biofilm growth. ..
  17. SPECTROSCOPY OF BIOMEMBRANE MONOLAYERS
    Richard Dluhy; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  18. iNOS, COX-2, and Arginase in Murine Barrett's Esophagus
    Keith Wilson; Fiscal Year: 2003
    ..We will study macroscopic and histologic evidence of esophagitis, BE and BAA, gene expression, apoptosis and proliferation in A. wild-type (WT) vs. COX-2-/- mice, B. W'I- vs. iNOS -/-mice, and C. WT vs. argll -/- mice. ..
  19. SPECTROSCOPY OF BIOMEMBRANE MONOLAYERS
    Richard Dluhy; Fiscal Year: 2002
    ..abstract_text> ..
  20. Orgin of multidrug resistant uropathogenic E. coli
    Lee Riley; Fiscal Year: 2008
    ..This knowledge should lead to the implementation of improved control of drug-resistant infectious diseases. ..
  21. Detection of Pathogens in Avian Fecal Pollution
    SANDRA MCLELLAN; Fiscal Year: 2003
    ..abstract_text> ..
  22. Identifying Autism Susceptibility Genes By High-Throughput Chip Resequencing
    Michael Zwick; Fiscal Year: 2009
    ..Identifying the genetic basis of autism could enable more rapid diagnostic testing and provide insight into the root causes and eventually pave the way for better treatments of this increasingly common disorder. ..
  23. MOLECULAR BIOLOGY OF INFECTION WITH M TUBERCULOSIS
    Lee Riley; Fiscal Year: 2002
    ..In addition, these studies may identify several potential targets for new chemotherapeutic agents or vaccine candidates. ..
  24. Social and biomedical risk factors for multi-drug resistant TB in rural China
    Biao Xu; Fiscal Year: 2010
    ..It is imperative to find not only biological factors but also social factors that more distally fuel this ongoing epidemic. Results of this study can contribute directly to improving TB control strategies aimed at reducing MDR-TB. ..
  25. LIPOSOMAL S MUTANS DELIVERY SYSTEM FOR CARIES IMMUNITY
    Noel Childers; Fiscal Year: 2001
    ....
  26. VIRULENCE FACTORS OF GROUP B STREPTOCOCCI
    John Bohnsack; Fiscal Year: 2001
    ..This approach will ultimately contribute to understanding the molecular basis of disease caused by GBS, and help devise strategies for preventing and treating this important cause of serious human neonatal bacterial infection. ..
  27. Helicobacter induced hepatitis and tumorigenesis
    James Fox; Fiscal Year: 2009
    ..A more complete understanding of the etiopathogenesis of hepatobiliary disease and hepatocellular carcinoma is likely to result in new strategies for treatment and prevention of these important human diseases. ..
  28. Extracrevicular Invasion by Periodontal Pathogens
    JOEL RUDNEY; Fiscal Year: 2005
    ..abstract_text> ..
  29. High-throughput Sequencing by Pyrosequencing
    Mostafa Ronaghi; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  30. Effects of Artemisinin on Leishmania SERCA pump
    JOHN MEADE; Fiscal Year: 2008
    ..cruzi and T. brucei SERCAs. [unreadable] [unreadable] [unreadable]..
  31. Clinical Impact of Quinolone-Resistant E.coli carriage
    Ebbing Lautenbach; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  32. OmpR and acid regulation of Escherichia coli fim genes
    WILLIAM SCHWAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  33. IN VIVO STUDIES OF SALIVA ANTIMICROBIAL PROTEINS
    JOEL RUDNEY; Fiscal Year: 2001
    ..By focusing on redundancy and complexing, this project also addresses the question of whether individual proteins exert unique and specific effects in the mouth. ..
  34. Tissue-Specific Infection by Group A Streptococci
    DEBRA BESSEN; Fiscal Year: 2007
    ..A better understanding of the molecular basis for throat- and skin-specific infection might aid in the development of vaccines which target essential adaptive traits and thereby, break the chain of transmission. ..
  35. Proline uptake in staphylococcus aureus pathogenesis
    WILLIAM SCHWAN; Fiscal Year: 2002
    ..The results of this study will help us understand the role of proline transport in S. aureus infections. ..
  36. Quinolone resistant E coli in long term care facilities
    Ebbing Lautenbach; Fiscal Year: 2009
    ..abstract_text> ..
  37. Population genomics of Streptococcus pyogenes
    DEBRA BESSEN; Fiscal Year: 2009
    ..abstract_text> ..
  38. Novel Application of Infection Control Strategies to Limit Transmission of ESBL'S
    Ebbing Lautenbach; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  39. Molecular Evolution of Resistance in Strep. pyogenes
    DEBRA BESSEN; Fiscal Year: 2005
    ..GAS provide a sound model system for understanding the molecular evolution underlying the early stages of the emergence and global spread of antibiotic resistance. ..
  40. QUINOLONE RESISTANCE IN NOSOCOMIAL URINARY INFECTIONS
    Ebbing Lautenbach; Fiscal Year: 2004
    ....
  41. EVOLUTION OF GROUP A STREPTOCOCCI
    DEBRA BESSEN; Fiscal Year: 2004
    ..These sera will be tested to determine whether strain structure is determined by the immunogenicity of the emm and sof gene products. ..
  42. PATHOGENESIS OF CHLAMYDIAL OCULAR INFECTIONS
    Deborah Dean; Fiscal Year: 2004
    ..A greater understanding of the pathogenesis of trachoma will aid in developing public health interventions and a vaccine that can be implemented in trachoma endemic areas throughout the world. ..
  43. C. trachomatis genomics, strain typing, and evolution
    Deborah Dean; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  44. Pathogenesis of Persistent Chlamydial STDs
    Deborah Dean; Fiscal Year: 2007
    ..1 Description, ..