plastic embedding

Summary

Summary: The infiltrating of histological specimens with plastics, including acrylic resins, epoxy resins and polyethylene glycol, for support of the tissues in preparation for sectioning with a microtome.

Top Publications

  1. ncbi A study of neovascularization in the rat ischemic hindlimb using Araldite casting and Spalteholtz tissue clearing
    Peter E Westerweel
    Department of Vascular Medicine, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    Cardiovasc Pathol 14:294-7. 2005
  2. ncbi Bone histomorphometry in arthritis models
    Georg Schett
    Methods Mol Med 135:269-83. 2007
  3. ncbi Correlative light and electron microscopy using immunolabeled resin sections
    Heinz Schwarz
    Max Planck Institut fur Entwicklungsbiologie, Tuebingen, Germany
    Methods Mol Biol 369:229-56. 2007
  4. ncbi Rapid diagnostic thin section electron microscopy of bacterial endospores
    Michael Laue
    Centre for Biological Safety 4, Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
    J Microbiol Methods 70:45-54. 2007
  5. ncbi Triton X-100 pretreatment of LR-white thin sections improves immunofluorescence specificity and intensity
    Salem S Ghrebi
    Department of Oral Biological and Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada
    Microsc Res Tech 70:555-62. 2007
  6. ncbi Rapid plastic embedding is compatible with colorimetric detection following whole mount in situ hybridization in plant specimens
    A J Reid
    Department of Biology, University of Calgary, AB, Canada
    Biotech Histochem 76:3-9. 2001
  7. ncbi A novel method for correlating internal and external structure of individual myelinated nerve fibres
    Z Cai
    Department of Neurology and University Department of Neurology, Royal Adelaide Hospital, Adelaide, Australia
    J Neurosci Methods 105:39-43. 2001
  8. ncbi Ciliated epithelium-lined radicular cysts
    P N Ramachandran Nair
    Department of Oral Structural Biology, Center of Dental and Oral Medicine, University of Zurich, Switzerland
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:485-93. 2002
  9. ncbi Freeze-dried and resin-embedded biological material is well suited for ultrastructure research
    L Edelmann
    Anatomie und Zellbiologie, Universitat des Saarlandes, D 66421 Homburg Saar, Germany
    J Microsc 207:5-26. 2002
  10. ncbi Embedding in Spurr's resin is a good choice for immunolabelling after freeze drying as shown with chemically unfixed dendritic cells
    D Spehner
    INSERM EPI 99 08, EFS Alsace, 10 rue Spielmann 67065 Strasbourg, France
    J Microsc 207:1-4. 2002

Research Grants

  1. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2005
  2. Electron Tomography of Adhesive Junctions
    David Stokes; Fiscal Year: 2006
  3. Adverse Effects of Noise on Hearing: Basic Mechanisms
    Barbara A Bohne; Fiscal Year: 2010
  4. CORE--BONE MORPHOMETRY AND MOLECULAR IMAGING
    Robert Weinstein; Fiscal Year: 2001
  5. A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION
    Andreas Hoenger; Fiscal Year: 2010
  6. A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION
    Andreas Hoenger; Fiscal Year: 2010
  7. A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION
    Andreas Hoenger; Fiscal Year: 2009
  8. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2002
  9. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2001
  10. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2000

Detail Information

Publications154 found, 100 shown here

  1. ncbi A study of neovascularization in the rat ischemic hindlimb using Araldite casting and Spalteholtz tissue clearing
    Peter E Westerweel
    Department of Vascular Medicine, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    Cardiovasc Pathol 14:294-7. 2005
    ..We explored an alternative strategy to corrosion casting for visualizing neovascularization in a rat hindlimb ischemia model...
  2. ncbi Bone histomorphometry in arthritis models
    Georg Schett
    Methods Mol Med 135:269-83. 2007
    ..Finally, evaluation of arthritic paw sections by histomorphometry, a semiautomated computerized method to measure areas of interest, is described...
  3. ncbi Correlative light and electron microscopy using immunolabeled resin sections
    Heinz Schwarz
    Max Planck Institut fur Entwicklungsbiologie, Tuebingen, Germany
    Methods Mol Biol 369:229-56. 2007
    ..Subsequent sections, labeled with immunogold, are analyzed by electron microscopy to identify the label within the cellular architecture at high resolution...
  4. ncbi Rapid diagnostic thin section electron microscopy of bacterial endospores
    Michael Laue
    Centre for Biological Safety 4, Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
    J Microbiol Methods 70:45-54. 2007
    ..Because of its universality and speed the described protocol complements negative staining electron microscopy as a front line method for the morphology-based diagnosis of pathogens in environmental and clinical samples...
  5. ncbi Triton X-100 pretreatment of LR-white thin sections improves immunofluorescence specificity and intensity
    Salem S Ghrebi
    Department of Oral Biological and Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada
    Microsc Res Tech 70:555-62. 2007
    ..This novel technique could be applied in many research areas where postembedding fluorescent immunolabeling with higher labeling intensity is desired...
  6. ncbi Rapid plastic embedding is compatible with colorimetric detection following whole mount in situ hybridization in plant specimens
    A J Reid
    Department of Biology, University of Calgary, AB, Canada
    Biotech Histochem 76:3-9. 2001
    ..R. White resin containing 10% (v/v) polyethylene glycol 400, and heat polymerized. The addition of the plasticizer allows L.R. White embedded tissues to be sectioned at 10 microm providing excellent signal contrast...
  7. ncbi A novel method for correlating internal and external structure of individual myelinated nerve fibres
    Z Cai
    Department of Neurology and University Department of Neurology, Royal Adelaide Hospital, Adelaide, Australia
    J Neurosci Methods 105:39-43. 2001
    ..This method is particularly useful for the correlative study of the myelin-axon relationships...
  8. ncbi Ciliated epithelium-lined radicular cysts
    P N Ramachandran Nair
    Department of Oral Structural Biology, Center of Dental and Oral Medicine, University of Zurich, Switzerland
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:485-93. 2002
    ..This report describes 3 cases of ciliated epithelium-lined radicular cysts among 256 apical periodontitis lesions and also illustrates the occurrence of an Actinomyces-infected periapical cyst...
  9. ncbi Freeze-dried and resin-embedded biological material is well suited for ultrastructure research
    L Edelmann
    Anatomie und Zellbiologie, Universitat des Saarlandes, D 66421 Homburg Saar, Germany
    J Microsc 207:5-26. 2002
    ..Sublimation studies with differently hydrated and cryofixed macromolecules are suggested to settle this issue...
  10. ncbi Embedding in Spurr's resin is a good choice for immunolabelling after freeze drying as shown with chemically unfixed dendritic cells
    D Spehner
    INSERM EPI 99 08, EFS Alsace, 10 rue Spielmann 67065 Strasbourg, France
    J Microsc 207:1-4. 2002
    ..Furthermore, we demonstrate the feasibility of protein localization in ultrathin sections by immunolabelling of the major histocompatibility class II molecules...
  11. ncbi High resolution micromill sampling for analysis of fish otoliths by ICP-MS: effects of sampling and specimen preparation on trace element fingerprints
    Zikri Arslan
    Jackson State University, Department of Chemistry, P O Box 17910, Jackson, MS 39217, USA
    Mar Environ Res 66:364-71. 2008
    ..Bias on Zn was larger that could limit its application in discriminating individuals...
  12. ncbi Correlative light/electron microscopy for the investigation of microbial mats from Black Sea Cold Seeps
    Christoph Wrede
    Institut für Mikrobiologie und Genetik der Universität Göttingen, Grisebachstr 8, D 37077 Gottingen, Germany
    J Microbiol Methods 73:85-91. 2008
    ..This allows lectin mapping at low resolution for light microscopy in direct comparison with a highly resolved electron microscopic image...
  13. ncbi A simple method for orienting silk and other flexible fibres in transmission electron microscopy specimens
    J E Trancik
    University of Oxford, Department of Materials, Parks Road, Oxford OX1 3PH, UK
    J Microsc 203:235-8. 2001
    ..Both transverse and longitudinal sections can then be cut with a microtome. The method can be extended to obtain sections at any intermediate orientation...
  14. ncbi The effect of dental implant spacing on peri-implant bone using the rabbit (Oryctolagus cuniculus) tibia model
    C L Hatley
    Staff Prosthodontist, U.S. Army Dental Activity, Fort Stewart, GA, USA
    J Prosthodont 10:154-9. 2001
    ..CONCLUSION: Within the limits of this study, it seems placing implants closely together does not adversely affect bone height or density. Conversely, it seems that placing implants closer together may increase bone growth...
  15. ncbi Electron microscopic studies of ion- and H2O-transporting epithelial cells in the horizontal ampulla of the pigeon
    H Villegas
    Institute of Anatomy, , Germany
    Histol Histopathol 16:1161-74. 2001
    ..The question whether the proteins are dispersed in the endolymph or contribute to cupula formation remains unclear. The morphology and possible functions of these two cell types are discussed on the basis of electron microscopic results...
  16. ncbi [Application of double immunostaining in resin-embedded bone marrow biopsy specimens]
    Hong Yan Wu
    Zhonghua Bing Li Xue Za Zhi 36:853-4. 2007
  17. ncbi Histologic and radiographic assessment of caries-like lesions localized at the crown margin
    Axel Zoellner
    Faculty of Dentistry, University of Witten Herdecke, Witten, Germany
    J Prosthet Dent 88:54-9. 2002
    ..Little is known about the impact of crown margins on the demineralization and remineralization process or on the interpretation of radiographs for the detection of early secondary caries...
  18. pmc Site-specific 3D imaging of cells and tissues with a dual beam microscope
    Jurgen A W Heymann
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD, USA
    J Struct Biol 155:63-73. 2006
    ..Our results demonstrate that dual beam imaging is a powerful new tool for cellular and subcellular imaging in 3D for both basic biomedical and clinical applications...
  19. ncbi Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels
    Richard A Coleman
    Physiology Department, University of Maryland School of Medicine, Baltimore Street, Baltimore, MD 21201, USA
    J Histochem Cytochem 54:817-27. 2006
    ..In demonstration of the methods, we describe the localization of the thiazide-sensitive sodium/chloride cotransporter and the epithelial sodium channel in rat kidney...
  20. pmc How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies
    T Krenacs
    Laboratory of Tumour Pathology and Molecular Diagnostics, Institute for Biotechnology, Bay Zoltan Foundation for Applied Research, H 6726 Szeged, Derkovits fasor 2, Hungary
    J Clin Pathol 58:897-903. 2005
    ..By meeting traditional and contemporary demands on the haematopathologist, it offers a powerful alternative to paraffin wax processing for diagnosis and research...
  21. ncbi A 16-year study of the microgap between 272 human titanium implants and their abutments
    Antonio Scarano
    Dental School, University of Chieti Pescara, Chieti, Italy
    J Oral Implantol 31:269-75. 2005
    ..In these retrieved implants, the size of the microgap was markedly variable and much larger than that observed in vitro...
  22. ncbi A new technique for hard tissues
    W Morrow
    University of Missouri Kansas City, School of Medicine, Basic Medical Science, 64108, USA
    Biotech Histochem 77:43-5. 2002
    ..I outline here two simple techniques for producing stained, ground, thin sections using readily available Romanowsky-type cytology stains and a urine sediment stain. Results are comparable to hematoxylin and eosin stained specimens...
  23. ncbi Plastination of dissected brain specimens and Mulligan-stained sections of the human brain
    F M Baeres
    Institute of Medical Anatomy, University of Copenhagen, Copenhagen, Denmark
    Eur J Morphol 39:307-11. 2001
    ....
  24. ncbi Subcellular localization of GABA receptors in the central nervous system using post-embedding immunohistochemistry
    Wendy L Heck
    Department of Biology and Cell Molecular Physiology, University of North Carolina Chapel Hill, Chapel Hill, NC 27599, USA
    Brain Res Brain Res Protoc 9:173-80. 2002
    ..Therefore, unlike many protocols for post-embedding immunoelectronmicroscopy, this method allows for superior preservation of tissue ultrastructure compared to results previously published by others...
  25. ncbi Autometallographic localization of heavy metals in resin sections, cryosections and isolated cells of the mussel Mytilus galloprovincialis (L.)
    E K Raftopoulou
    Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece
    Histochem J 34:583-8. 2002
    ....
  26. ncbi Aortic arch variation analyzed by using plastination
    Mircea Constantin Sora
    Plastination Laboratory, Institute of Anatomy, Vienna University, Vienna, Austria
    Clin Anat 15:379-82. 2002
    ..The caliber and length of the main branches of the aortic arch were determined and compared to reports in the literature. This variation was discovered in the context of producing transverse body slices using an E12 plastination process...
  27. ncbi An improved electron microscopic technique for the immunolabeling of Cryptosporidium parvum oocysts
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 92:403-5. 2006
    ..The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa...
  28. pmc Tissue-plastinated vs. celloidin-embedded large serial sections in video, analog and digital photographic on-screen reproduction: a preliminary step to exact virtual 3D modelling, exemplified in the normal midface and cleft-lip and palate
    Constantin A Landes
    Maxillofacial and Facial Plastic Surgery, J W Goethe University Medical Centre, Frankfurt, Germany
    J Anat 207:175-91. 2005
    ..Digital photography is preferred for on-screen analysis...
  29. ncbi Demonstration of intravital microfissures in undecalcified plastic-embedded temporal bones with the prestaining technique
    T Frisch
    Department of Otolaryngology-Head and Neck Surgery, Rigshospitalet, Copenhagen, Denmark
    Ann Otol Rhinol Laryngol 110:749-57. 2001
    ..The importance of microfissures in the petrous bone in the causation of otosclerosis and perilymphatic leakage has long been discussed, and the present techniques may advance our understanding of these pathological conditions..
  30. ncbi Enhancement of immunogold-labelled focal adhesion sites in fibroblasts cultured on metal substrates: problems and solutions
    G R Owen
    AO Research Institute, Clavadelerstrasse, Davos Platz, Switzerland
    Cell Biol Int 25:1251-9. 2001
    ..Therefore, comparative studies of cell adhesion to different biomaterial substrates can be performed using immunogold-labelling with gold enhancement...
  31. ncbi Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomography
    Peter Konig
    Department of Biochemistry and Biophysics, The Howard Hughes Medical Institute, University of California, San Francisco, CA 94143 2240, USA
    Ultramicroscopy 103:261-74. 2005
    ..The implications of the new method for the preparation of other difficult samples and additional application possibilities are discussed...
  32. ncbi Postembedding ultrastructural in situ hybridization on ultrathin cryosections and LR white resin sections
    P Mandry
    GSF Institute of Pathology, , Germany
    Ultrastruct Pathol 17:185-94. 1993
    ..EBV DNA was detected in the nucleus and/or cytoplasm in 10% to 20% of P3HR1 cells. A similar percentage of cells in thin L-sectioned material prepared by routine methods showed virus particles at different stages of maturation...
  33. ncbi Optimization of glycol methacrylate embedding of large specimens in neurological research. Study of rat skull-brain specimens after implantation of polyester meshes
    Ralf Quester
    Faculty of Medicine, Center for Neurosurgery, Stereotactic and Functional Neurosurgery Clinic, University of Cologne, Joseph Stelzmann Strasse 9, 50931 Koln, Germany
    J Neurosci Methods 113:15-26. 2002
    ....
  34. ncbi Cell-free extract systems and the cytoskeleton: preparation of biochemical experiments for transmission electron microscopy
    Margaret Coughlin
    Department of Systems Biology, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 369:199-212. 2007
    ..Working in vitro opens these complex processes to biochemical dissection. Here, we describe methods to view these in vitro preparations by thin-section electron microscopy...
  35. ncbi Processing plant tissues for ultrastructural study
    John Kuo
    Centre for Microscopy and Microanalysis, The University of Western Australia, Western Australia, Australia
    Methods Mol Biol 369:35-45. 2007
    ..Additional vacuum infiltration during resin infiltration procedure to improve the efficiency of resin penetration is implemented...
  36. ncbi Staining of the Reinke crystalloids in the human testis--re-evaluation study
    Nobuyuki Nakamura
    Department of Urology, School of Medicine, Fukuoka University, 7 45 1 Nanakuma, Johnan ku, Fukuoka City, Fukuoka, 814 0180 Japan
    Okajimas Folia Anat Jpn 82:103-10. 2005
    ..The reasons for stainability and/or non stainability of CRs with hematoxylin, eosin and other dyes are discussed...
  37. ncbi Biological staining: mechanisms and theory
    R W Horobin
    Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, The University of Glasgow, UK
    Biotech Histochem 77:3-13. 2002
    ..The QSAR correlates with staining of endoplasmic reticulum, lysosomes, mitochondria, DNA, or the plasma membranes of living cells...
  38. ncbi Vasa vasorum of the human great saphenous vein
    D Kachlik
    Department of Anatomy, 3rd Medical Faculty of Charles University, Ruska 87, 100 00 Prague 10, Czech Republic
    Surg Radiol Anat 24:377-81. 2003
    ..No lymphatic was found in any of the layers of GSV wall. From the findings the authors recommend extremely careful dissection of the GSV wall during in situ grafting surgery, to ensure the best viability of the venous wall...
  39. pmc Co-localization and functional cross-talk between A1 and P2Y1 purine receptors in rat hippocampus
    I Tonazzini
    Department of Psychiatry Neurobiology Pharmacology and Biotechnology, University of Pisa, 56126, Pisa, Italy
    Eur J Neurosci 26:890-902. 2007
    ..This may be particularly important during pathological conditions, when large amounts of these mediators are released...
  40. ncbi Harder and stiffer bone osseointegrated to roughened titanium
    F Butz
    The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, 10833 Le Conte Avenue B3 087 CHS, Box 951668, Los Angeles, CA 90095 1668, USA
    J Dent Res 85:560-5. 2006
    ....
  41. ncbi Quadriplegic areflexic ICU illness: selective thick filament loss and normal nerve histology
    Howard W Sander
    Department of Neurology, Peripheral Neuropathy Center, Weill College of Medicine of Cornell University, 635 Madison Ave, Suite 400, New York, New York 10022, USA
    Muscle Nerve 26:499-505. 2002
    ..This suggests that areflexic ICU quadriplegia is a primary myopathy and not an axonal polyneuropathy...
  42. ncbi Improvements in histological quality and signal retention following in situ hybridization in early chick embryos using plastic resin and recolorization
    A L Sawitzke
    Division of Natural Sciences, Salt Lake Community College, 4600 S Redwood Road, Salt Lake City, Utah 84123, USA
    Biotech Histochem 80:35-41. 2005
    ....
  43. ncbi Comparative studies of the cell structures of Mycobacterium leprae and M. tuberculosis using the electron microscopy freeze-substitution technique
    Akemi Takade
    Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
    Microbiol Immunol 47:265-70. 2003
    ..The cell widths measured on electron micrographs were 0.44 microm for M. tuberculosis and 0.38 microm for M. leprae. The peptidoglycan layer of M. leprae was 4-5 nm, while the corresponding layer of M. tuberculosis was 10-15 nm...
  44. ncbi Apoptotic changes of Middle East jaw lymphoma
    Raja Kummoona
    Iraqi Board of Medical Specializations, Medical Avenue America 38 2 887, PO Box 65066, Baghdad, Iraq
    J Craniofac Surg 17:231-5. 2006
    ..The presence of low-level of apoptosis is a poor prognostic feature of jaw lymphoma due to homogeneity and degenerative changes of the mitochondria and disruption of the cell surface...
  45. doi Progressive hearing loss in mice with a mutated vitamin D receptor gene
    Jing Zou
    Department of Otolaryngology, School of Medicine, University of Tampere, Tampere, Finland
    Audiol Neurootol 13:219-30. 2008
    ..Hormonal forms of vitamin D regulate transcription by binding with the high-affinity vitamin D receptor (VDR)...
  46. ncbi Uptake of 111In-Z2D3 on SPECT imaging in a swine model of coronary stent restenosis correlated with cell proliferation
    Lynne L Johnson
    Division of Cardiology, Department of Medicine, Brown University, Rhode Island Hospital, MB Room 209, 593 Eddy Street, Providence, RI 02903, USA
    J Nucl Med 45:294-9. 2004
    ..We hypothesized that an antibody linked to polymers to increase specific radioactivity can be visualized on SPECT images and that counts in the target will correlate with the strength of the biologic signal...
  47. ncbi From blastocyst to placenta: the morphology of implantation in the baboon
    A C Enders
    Department of Cell Biology and Human Anatomy, School of Medicine, and California Regional Primate Research Center, University of California, Davis 95616, USA
    Hum Reprod Update 3:561-73. 1997
    ..All tissues were prepared for electron microscopy by aldehyde fixation and plastic embedding. Maturation of trophoblast from the compacted morula stage to the expanded blastocyst stage includes ..
  48. ncbi Cytokine accumulation in osteitis fibrosa of renal osteodystrophy
    M E L Duarte
    Programa Avançado de Biologia Celular Aplicado à Medicine PABCAM, Hospital Universitario Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
    Braz J Med Biol Res 35:25-9. 2002
    ..necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) using an undecalcified plastic embedding method...
  49. ncbi Freeze-substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 degrees C
    C Buser
    Zentrale Einrichtung Elektronenmikroskopie, Universitaet Ulm, Albert Einstein Allee 11, 89069 Ulm, Germany
    J Microsc 230:268-77. 2008
    High-pressure freezing followed by freeze substitution and plastic embedding is becoming a more widely used method for TEM sample preparation...
  50. doi A comparison of fixation methods on lung morphology in a murine model of emphysema
    S Braber
    Utrecht Institute for Pharmaceutical Sciences, Utrecht Univ, The Netherlands
    Am J Physiol Lung Cell Mol Physiol 299:L843-51. 2010
    ..lung morphology for lung emphysema analysis when embedded in paraffin, while for Carnoy's fixed lungs, plastic embedding was preferred...
  51. ncbi Bone histomorphometry in three females with Rett syndrome
    S S Budden
    Division of Developmental Pediatrics, Oregon Health Sciences University, P O Box 574, Portland, OR 97207, USA
    Brain Dev 23:S133-7. 2001
    ..Samples were processed for plastic embedding, sectioned, stained, and histomorphometry performed in the cancellous bone...
  52. ncbi Three-dimensional distributions of elements in biological samples by energy-filtered electron tomography
    R D Leapman
    Division of Bioengineering and Physical Science, ORS, National Institutes of Health, Building 13, 3N17, Bethesda, MD 20892, USA
    Ultramicroscopy 100:115-25. 2004
    ..of the nematode, Caenorhabditis elegans were prepared by high-pressure freezing, freeze-substitution and plastic embedding. Images were recorded at energy losses above and below the phosphorus L2,3 edge using a post-column imaging ..
  53. ncbi Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayers
    N Jimenez
    Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    J Microsc 221:216-23. 2006
    ..the artefacts induced by conventional chemical fixation, and, in combination with freeze-substitution and plastic embedding, is a reliable method for the ultrastructural analysis of mammalian cell monolayers...
  54. ncbi The use of filter membranes for high-pressure freezing of cell monolayers
    M K Morphew
    The Boulder Laboratory for 3 D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Microsc 212:21-5. 2003
    Rapid freezing of cells and tissues, followed by freeze-substitution fixation and plastic embedding, has become a highly reliable method for preparing samples for imaging in the electron microscope...
  55. ncbi A comparison of methods for the estimation of retroviral burden
    S T Bierley
    IEM Section, Pathology Associates International, Durham NC, USA
    Dev Biol Stand 88:163-5. 1996
    ..To validate further the plastic embedding with thin sectioning, we evaluated cell culture supernatants (pellets) for retroviral burden at various ..
  56. ncbi Electron microscopic contrast of the cytoskeleton and junctional complexes of intestinal epithelial cells by ethanolic phosphotungstic acid
    A Tato
    Central Service for Support to Experimental Research, University of Valencia, Burjassot, Spain
    Eur J Morphol 38:134-41. 2000
    After glutaraldehyde fixation and treatment with ethanolic phosphotungstic acid (E-PTA) before plastic embedding, sections of rat large intestine showed a characteristic electron contrasting pattern in epithelial cells...
  57. pmc Cytomegalovirus primary envelopment occurs at large infoldings of the inner nuclear membrane
    Christopher Buser
    Universitatsklinikum Ulm, Institut fur Virologie, Albert Einstein Allee 11, 89081 Ulm, Germany
    J Virol 81:3042-8. 2007
    ..cytomegalovirus by transmission electron microscopy after high-pressure freezing, freeze substitution, and plastic embedding. We observed large tubular infoldings of the inner nuclear membrane that were free of lamina and active in ..
  58. ncbi Temporal development of 2',3'-dideoxyinosine (ddI)-induced peripheral myelinopathy
    T A Patterson
    Division of Neurotoxicology, National Center for Toxicological Research FDA, 72079 9502, Jefferson, AR, USA
    Neurotoxicol Teratol 22:429-34. 2000
    ..by immersion in neutral phosphate-buffered formalin, dehydrated in graded alcohols, and embedded in plastic embedding media. One-micrometer-thick sections were cut and stained with toluidine blue and basic fuchsin...
  59. ncbi The low temperature vacuum embedding technique for X-ray microanalysis of the developing inner ear
    S O Wikström
    Department of Otolaryngology, Karolinska Hospital, Stockholm, Sweden
    Acta Otolaryngol 105:223-31. 1988
    ..With plastic embedding, an improvement of the morphological resolution was achieved, which allows reliable structural ..
  60. ncbi Apoptosis in male germinal line cells of rat in vivo: caused by phosphamidon
    M A Akbarsha
    Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India
    Cytobios 91:33-44. 1997
    ..It is suggested that plastic embedding and semithin sectioning are appropriate methodologies to identify apoptotic cells in histological ..
  61. doi Diagnosis of unknown nonhematological tumors by bone marrow biopsy: a retrospective analysis of 10,112 samples
    Li Xiao
    Department of Hematology, the Sixth Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China
    J Cancer Res Clin Oncol 135:687-93. 2009
    ..To investigate how unselected bone marrow (BM) biopsy examinations could help in the diagnosis of clinically unknown nonhematological tumors...
  62. doi Making the most of bone marrow trephine biopsy
    Bridget S Wilkins
    Guy s and St Thomas Hospitals NHS Foundation Trust, London, King s College, London and Newcastle University, Newcastle, UK
    Histopathology 55:631-40. 2009
    ..The requirement for decalcification or plastic embedding during tissue processing poses additional challenges for histological quality and successful application of ..
  63. ncbi Three-dimensional analysis of molecular signals with episcopic imaging techniques
    Wolfgang J Weninger
    Integrative Morphology Group, Medical University of Vienna, Austria
    Methods Mol Biol 411:35-46. 2007
    ..In contrast, the plastic embedding medium used in HREM is strongly fluorescent, and tissue architecture is detected at the surface because of ..
  64. doi Semi-thin sections of epoxy resin-embedded mouse embryos in morphological analysis of whole mount in situ RNA hybridization
    D Mitrecic
    Croatian Institute for Brain Research, School of Medicine, Salata 12, HR 10000 Zagreb, Croatia
    J Microsc 232:504-7. 2008
    ..Therefore, use of plastic embedding media, which allow semi-thin and ultra-thin sectioning for light and electron microscopy, could be an ..
  65. pmc Early effect of a single intravenous injection of ethanol on hepatic sinusoidal endothelial fenestrae in rabbits
    Frank Jacobs
    Center for Molecular and Vascular Biology, Department of Molecular and Cellular Medicine, University of Leuven, Herestraat 49, Leuven, 3000, Belgium
    Comp Hepatol 8:4. 2009
    ..The effect of ethanol on the size of fenestrae was studied using transmission electron microscopy, because plastic embedding provides true measures for the diameter of fenestrae...
  66. doi Cryo-electron tomography in biology and medicine
    Roman I Koning
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P O Box 9600, 2300 RC, Leiden, The Netherlands
    Ann Anat 191:427-45. 2009
    ..The use of cryo-preparation and imaging techniques eliminates artifacts induced by plastic embedding and staining of the samples is circumvented...
  67. doi Low-temperature glycol methacrylate resin embedding method: A protocol suitable for bone marrow immunohistochemistry, PCR, and fish analysis
    QiGuo Zhang
    Department of Hematology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
    Microsc Res Tech 73:1067-71. 2010
    ..method, BM enzyme activity and antigenic determinants are well preserved, and moreover, DNA extracted from plastic embedding sections is suitable for PCR amplification and sequencing, FISH analysis can be well done because of the DNA ..
  68. ncbi Reflection contrast microscopy: The bridge between light and electron microscopy
    F A Prins
    Department of Pathology, Leiden University Hospital, The Netherlands
    Methods Mol Biol 319:363-401. 2006
    ..Therefore, plastic embedding is recommended.
  69. doi Labeling of ultrathin resin sections for correlative light and electron microscopy
    Gunar Fabig
    Center for Regenerative Therapies, TU Dresden, Fetscherstraße 105, D 01307 Dresden, Saxony, Germany
    Methods Cell Biol 111:75-93. 2012
    ..This way, fluorescent signals can be directly correlated to the corresponding subcellular structures in the area of interest...
  70. ncbi Taking public education seriously: BODY WORLDS, the science museum, and democratizing bioethics education
    Catherine Myser
    Am J Bioeth 7:34-6; discussion W1-3. 2007
  71. doi Correlative light and electron microscopy of intermediate stages of meiotic spindle assembly in the early Caenorhabditis elegans embryo
    Ina Woog
    Medical Theoretical Center MTZ, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Fiedlerstraße 42, 01307 Dresden, Germany
    Methods Cell Biol 111:223-34. 2012
    ..The ex-utero approach allows precise tracking of the developmental events in isolated meiotic embryos, thus facilitating the comparison of structural features between wild-type and mutant or RNAi-treated samples...
  72. ncbi Three-dimensional reconstruction of the ankle by means of ultrathin slice plastination
    Mircea Constantin Sora
    Plastination Laboratory, Center for Anatomy and Cell Biology, Medical University of Vienna, Austria
    Clin Anat 20:196-200. 2007
    ..Morphometric analysis indicated that the model did not significantly differ from a sample of cadaveric specimens. These data support the use of plastinates for generating tissues sections useful for 3D computerized modeling...
  73. ncbi Fluorescence-integrated transmission electron microscopy images: integrating fluorescence microscopy with transmission electron microscopy
    Paul A Sims
    Zoology Department, University of Wisconsin, Madison, WI, USA
    Methods Mol Biol 369:291-308. 2007
    ..This procedure preserves the structure and function of green fluorescent protein (GFP) as determined by immunogold labeling of GFP, when compared with GFP expression, both demonstrated in the same thin section...
  74. doi Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures
    Elena V Polishchuk
    Institute of Protein Biochemistry, Naples, Italy
    Methods Mol Biol 931:413-22. 2013
    ..Here, we present a detailed protocol that describes all of the "tricks" and know-hows involved in carrying out the crucial steps of a CLEM experiment...
  75. ncbi Conventional specimen preparation techniques for transmission electron microscopy of cultured cells
    John J Bozzola
    IMAGE Center, Southern Illinois University, Carbondale, IL, USA
    Methods Mol Biol 369:1-18. 2007
    ..Directions are given on how to prepare the most commonly used buffers, fixatives, enrobement media, and embedding resins. These methods may be applied to most cultured organisms, from bacteria to mammalian cells...
  76. doi Plastinated heart slices aid echocardiographic interpretation in the dog
    Alejandro Gomez
    Department of Anatomy and Comparative Pathology, Veterinary Faculty, University of Murcia, Spain
    Vet Radiol Ultrasound 53:197-203. 2012
    ..Because of the dilation of the right heart during the fixation process, it was not possible to obtain plastinated specimens in ventricular systole. This paper may be a reference atlas for assisting 2D echocardiography interpretation...
  77. ncbi Computer aided three-dimensional reconstruction and modeling of the pelvis, by using plastinated cross sections, as a powerful tool for morphological investigations
    Mircea Constantin Sora
    Plastination Laboratory, Center for Anatomy and Cell Biology, Plastination and Topografic Anatomy, Medical University of Vienna, Währingerstr 13 3, 1090, Vienna, Austria
    Surg Radiol Anat 34:731-6. 2012
    ..Although databases consisting of serial sections derived from frozen cadaver material exist, plastination represents an alternative method for developing anatomical data useful for computerized reconstruction...
  78. pmc Quantification and thickness correction of EFTEM phosphorus maps
    M A Aronova
    Division of Bioengineering and Physical Science, ORS, National Institutes of Health, Bethesda, MD 20892, USA
    Ultramicroscopy 107:232-44. 2007
    ..First, the single-scattering EELS distributions are obtained from specimens of pure carbon and plastic embedding material...
  79. pmc Lysosomal accumulation of SCMAS (subunit c of mitochondrial ATP synthase) in neurons of the mouse model of mucopolysaccharidosis III B
    Sergey Ryazantsev
    Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1737, USA
    Mol Genet Metab 90:393-401. 2007
    ..Samples used for cryo-EM and further processed by standard EM procedures (osmium tetroxide fixation and plastic embedding) showed the disappearance of the SCMAS fingerprint arrays and appearance in the same location of "zebra ..
  80. pmc Embedding, serial sectioning and staining of zebrafish embryos using JB-4 resin
    Jessica Sullivan-Brown
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
    Nat Protoc 6:46-55. 2011
    ..Overall, we reinforce that plastic embedding can provide higher resolution of cellular details and is a valuable tool for cellular and morphological ..
  81. pmc Use of micro-computed tomography to nondestructively characterize biomineral coatings on solid freeform fabricated poly (L-lactic acid) and poly ((ε-caprolactone) scaffolds in vitro and in vivo
    Eiji Saito
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA
    Tissue Eng Part C Methods 19:507-17. 2013
    ..The presence of a biomineral coating within the porous scaffolds was confirmed through plastic embedding and μ-CT techniques...
  82. ncbi A novel bone scraper for intraoral harvesting: a device for filling small bone defects
    Davide Zaffe
    Department of Anatomy and Histology, Section of Human Anatomy, University of Modena and Reggio Emilia, Modena, Italy
    Clin Oral Implants Res 18:525-33. 2007
    ..To evaluate histologically the morphology and characteristics of bone chips harvested intraorally by Safescraper, a specially designed cortical bone collector...
  83. ncbi Ultramicrotomy for biological electron microscopy
    Herbert K Hagler
    Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 369:67-96. 2007
    ..This chapter is designed to assist the beginner in microtomy in understanding more fully the terms and basic principles involved in producing ultrathin sections of most plastic-embedded biological specimens...
  84. ncbi Electron microscopy in situ hybridization: tracking of DNA and RNA sequences at high resolution
    Dusan Cmarko
    Institute of Cellular Biology and Pathology, Ist Faculty of Medicine, Charles University, Prague, Czech Republic
    Methods Mol Biol 369:213-28. 2007
    ..The post-embedding EM-ISH protocol that currently represents the most widely used technique is described in detail, with a special emphasis on the organization of the cell nucleus...
  85. ncbi Behavioral, histological, and ex vivo magnetic resonance imaging assessment of graded contusion spinal cord injury in mice
    Rebecca A Nishi
    CRF Spinal Cord Injury Core Research Facility, University of California, Irvine, California 92697 4540, USA
    J Neurotrauma 24:674-89. 2007
    ..These results give researchers more options in how to analyze spinal cord injuries in animal models...
  86. ncbi Influence of cervical preflaring on determination of apical file size in maxillary molars: SEM analysis
    José Roberto Vanni
    Faculty of Dentistry, University of Passo Fundo FOUPF, Passo Fundo, RS, Brazil
    Braz Dent J 16:181-6. 2005
    ..Canals preflared with LA Axxess burs showed a more accurate binding of the files to the anatomical diameter at the WL in the mesiobuccal roots of maxillary first molars...
  87. ncbi In vivo and in vitro effects of an Er:YAG laser, a GaAlAs diode laser, and scaling and root planing on periodontally diseased root surfaces: a comparative histologic study
    Frank Schwarz
    Department of Oral Surgery, Heinrich Heine University, 40225 Dusseldorf, Germany
    Lasers Surg Med 32:359-66. 2003
    ....
  88. ncbi Immunohistochemical study during healing of free palatal mucosa grafts on plastic-embedded samples
    G Lauer
    Department of Oral and Maxillofacial Surgery, University Hospital, Carl-Gustav-Carus, Dresden, Germany
    J Oral Pathol Med 30:104-12. 2001
    ....
  89. ncbi Localization of a G protein Gi2 in the cilia of rat ependyma, oviduct and trachea
    H Shinohara
    Department of Anatomy, Mie University School of Medicine, Tsu, Japan
    Eur J Neurosci 10:699-707. 1998
    ..These findings suggest that Gi2 might play an important role in the signal transduction in ciliary membrane-associated function(s) of the ependymal cells, oviduct and trachea...
  90. ncbi A three-dimensional study of brain string vessels using celloidin sections stained with anti-collagen antibodies
    V R Challa
    Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Neurol Sci 203:165-7. 2002
    ..This technique appears suitable for the study of large number of string vessels...
  91. ncbi Reclamation of acetone in plastination laboratories: a simple and inexpensive method
    G Grondin
    Departement de Chimie Biologie, Universite du Quebec a Trois Rivieres, Canada
    Acta Anat (Basel) 158:26-9. 1997
    ..In addition vaporized acetone released during the impregnation step of plastination is recaptured...
  92. ncbi Time scale and extent of neuronal and synaptic loss in the hippocampal formation of malnourished adult rats
    J P Andrade
    Department of Anatomy, Porto Medical School, Portugal
    Brain Res 718:1-12. 1996
    ..These findings indicate that the changes induced by protein deprivation progressively increase during the early phases of treatment and that they are already evident after 3 months of protein deprivation...
  93. ncbi Effects of 2.94 microm Er:YAG laser radiation on root surfaces treated in situ: a histological study
    Matthias Folwaczny
    Department of Operative Dentistry and Periodontology, Ludwig Maximilians University, Munich, Germany
    J Periodontol 74:360-5. 2003
    ..g., charring or fusion, to the irradiated root surface. The purpose of the present study was to examine the morphologic changes on root surfaces following Er:YAG laser irradiation in situ using histological observation...
  94. ncbi Fine reconstruction of the pancreatic ductular system at the onset of pancreatitis
    H Endo
    Department of Internal Medicine II, Shimane Medical University, Izumo, Japan
    Histol Histopathol 17:107-12. 2002
    ..However, there were no anastomoses found among the central lumina or acini...
  95. ncbi Minimal compression of ultrathin sections with use of an oscillating diamond knife
    D Studer
    M E Müller Institute for Biomechanics, University of Berne, P O Box 30, CH 3010 Bern, Switzerland
    J Microsc 197:94-100. 2000
    ..No additional cutting artefacts were observed in the micrographs when they were compared with sections produced without oscillating the knife...
  96. ncbi Celloidin mounting (embedding without infiltration) - a new, simple and reliable method for producing serial sections of high thickness through complete human brains and its application to stereological and immunohistochemical investigations
    H Heinsen
    Morphological Brain Research Unit, University of Wuerzburg, Josef Schneider Strasse 2, D 97080, Wuerzburg, Germany
    J Chem Neuroanat 20:49-59. 2000
    ..Details of a fractionator analysis of the total striatal neuron number are described and the possible quantitative analysis of parallel immunohistochemically stained sections is discussed...
  97. doi The microvascular anatomy of the metatarsal bones: a plastination study
    Björn Rath
    Department of Anatomy, University of Cologne, Joseph Stelzmann Str 9, 50931, Cologne, Germany
    Surg Radiol Anat 31:271-7. 2009
    ..The purpose of this study was to categorize and systematize the arterial supply of the metatarsal bones and furthermore the observation of arterial lesions after frequently performed forefoot surgeries...
  98. ncbi Immunohistochemical staining of plastic (methyl-methacrylate)-embedded bone marrow biopsies applying the biotin-free tyramide signal amplification system
    Ekkehard Kunze
    Department of Osteopathology and Haematopathology, University of Goettingen, D 37099 Goettingen, Germany
    Appl Immunohistochem Mol Morphol 16:76-82. 2008
    ....
  99. ncbi Recognition of basic fuchsin prestained microfissures of intravital origin with fluorescence microscopy: validation of a shortcut
    T Frisch
    Otopathological Laboratory, Department of Otolaryngology, Head and Neck Surgery F 2071, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen Ø, Denmark
    Eur Arch Otorhinolaryngol 258:55-60. 2001
    ..This may prove to be of particular value in the research into the etiology and pathogenesis of otosclerosis as well as perilymphatic fistulae...
  100. ncbi What a piece of work is man: the Body Worlds exhibit at the Milwaukee Public Museum
    Joan M Bedinghaus
    Department of Family and Community Medicine, Medical College of Wisconsin, Milwaukee, WI 53226 0509, USA
    WMJ 107:59-61. 2008
  101. ncbi Electron microscopic assessment of smeared or imprinted specimens of solid tumours
    J E Dahlstrom
    Department of Anatomical Pathology, ACT Pathology, The Canberra Hospital, and Canberra Clinical School, University of Sydney, Australia
    Pathology 33:226-9. 2001
    ..This technique ensures that well-preserved tissue is available for electron microscopy even when the sample size is very limited...

Research Grants45

  1. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2005
    ..Our goal is to provide information on possible mechanisms of noise damage in the cochlea as a basis for developing scientifically sound strategies for reducing noise-induced hearing loss in humans. ..
  2. Electron Tomography of Adhesive Junctions
    David Stokes; Fiscal Year: 2006
    ..In preliminary work, we have studied intact desmosomes from after freeze-substitution and plastic embedding of newborn mouse epidermis...
  3. Adverse Effects of Noise on Hearing: Basic Mechanisms
    Barbara A Bohne; Fiscal Year: 2010
    ..Our results will provide information on mechanisms of damage from moderate noise so that drugs that will prevent or minimize noise-induced hair-cell death can be developed. ..
  4. CORE--BONE MORPHOMETRY AND MOLECULAR IMAGING
    Robert Weinstein; Fiscal Year: 2001
    ..routine plastic embedding, sectioning and staining of undecalcified, tetracycline labeled bone for quantitative histological analysis; ..
  5. A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION
    Andreas Hoenger; Fiscal Year: 2010
    ..molecular resolution in 3-D, and large-scale 3-D imaging typically provided by freeze substitution and plastic embedding of cells and tissue...
  6. A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION
    Andreas Hoenger; Fiscal Year: 2010
    ..molecular resolution in 3-D, and large-scale 3-D imaging typically provided by freeze substitution and plastic embedding of cells and tissue...
  7. A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION
    Andreas Hoenger; Fiscal Year: 2009
    ..molecular resolution in 3-D, and large-scale 3-D imaging typically provided by freeze substitution and plastic embedding of cells and tissue...
  8. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2002
    ..of formalin-fixed, paraffin-embedded tissue sections; histochemical stains of crystal sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  9. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2001
    ..of formalin-fixed, paraffin-embedded tissue sections; histochemical stains of crystal sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  10. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2000
    ..of formalin-fixed, paraffin-embedded tissue sections; histochemical stains of crystal sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  11. Electron Tomography of Adhesive Junctions
    David Stokes; Fiscal Year: 2004
    ..In preliminary work, we have studied intact desmosomes from after freeze-substitution and plastic embedding of newborn mouse epidermis...
  12. Electron Tomography of Adhesive Junctions
    David Stokes; Fiscal Year: 2005
    ..In preliminary work, we have studied intact desmosomes from after freeze-substitution and plastic embedding of newborn mouse epidermis...
  13. Electron Tomography of Adhesive Junctions
    David Stokes; Fiscal Year: 2006
    ..In preliminary work, we have studied intact desmosomes from after freeze-substitution and plastic embedding of newborn mouse epidermis...
  14. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2001
    ..on formalin-fixed, paraffin-embedded tissue sections; histochemical stains on cryostat sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  15. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2000
    ..on formalin-fixed, paraffin-embedded tissue sections; histochemical stains on cryostat sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  16. CORE--NEUROPATHOLOGY
    Stephen De Armond; Fiscal Year: 2002
    ..on formalin-fixed, paraffin-embedded tissue sections; histochemical stains on cryostat sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  17. CORE--NEUROPATHOLOGY
    STEVE DEARMOND; Fiscal Year: 2001
    ..on formalin-fixed, paraffin-embedded tissue sections; histochemical stains on cryostat sections of muscle; plastic embedding and toluidine blue staining of thick sections of peripheral nerve; electron microscopy; and full autopsies ..
  18. CORE--ELECTRON MICROSCOPY
    JUDITH OGILVIE; Fiscal Year: 2002
    ..These services include tissue processing and plastic embedding, tissue preparation for scanning electron microscopy, sectioning of plastic embedded tissue for light and ..
  19. CORE--DI VIRAL VECTOR
    MICHEAL LAI; Fiscal Year: 2000
    ..Exceptions are those procedures (plastic embedding and EM) which require perfusion...
  20. CORE--DI VIRAL VECTOR
    MICHEAL LAI; Fiscal Year: 2002
    ..Exceptions are those procedures (plastic embedding and EM) which require perfusion...
  21. CORE--DI VIRAL VECTOR
    MICHEAL LAI; Fiscal Year: 2001
    ..Exceptions are those procedures (plastic embedding and EM) which require perfusion...
  22. CORE--DI VIRAL VECTOR
    MICHEAL LAI; Fiscal Year: 1999
    ..Exceptions are those procedures (plastic embedding and EM) which require perfusion...
  23. CORE--CELL MORPHOLOGY
    Paul McCray; Fiscal Year: 2001
    ..Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding sectioning and routine staining for Center investigators...
  24. CORE -- CELLULAR MORPHOLOGY
    Paul McCray; Fiscal Year: 2003
    ..Core are: l) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding, sectioning and routine staining for Center investigators...
  25. CORE--CELL MORPHOLOGY
    Paul McCray; Fiscal Year: 2002
    ..Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding sectioning and routine staining for Center investigators...
  26. CORE--CELL MORPHOLOGY
    Paul McCray; Fiscal Year: 1999
    ..Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding sectioning and routine staining for Center investigators...
  27. Bone Core
    Laurie McCauley; Fiscal Year: 2004
    ..In addition, new services of processing undecalcified osseous specimens will be initiated using plastic embedding techniques...
  28. CORE--CELL MORPHOLOGY
    Paul McCray; Fiscal Year: 2000
    ..Core are: 1) To provide appropriate methods of tissue fixation, processing, cryopreservation, paraffin or plastic embedding sectioning and routine staining for Center investigators...
  29. CORE--GENERAL SCIENTIFIC
    W Dietrich; Fiscal Year: 2002
    ..The Core will process tissues for plastic embedding for both light and electron microscopic analysis...
  30. Bone Core
    Laurie McCauley; Fiscal Year: 2009
    ..hard tissue specimens in paraffin in addition to processing undecalcified osseous specimens using plastic embedding techniques...
  31. Bone Core
    Laurie K McCauley; Fiscal Year: 2011
    ..hard tissue specimens in paraffin in addition to processing undecalcified osseous specimens using plastic embedding techniques...
  32. CORE--HISTOPATHOLOGY AND EXPERIMENTAL ANIMAL FACILITY
    Maarten Bosland; Fiscal Year: 2000
    ..cryostat sectioning, preparation of slides for immunohistochemistry and in situ hybridization and PCR, plastic embedding and slide preparation, routine conventional and (immuno)histochemical staining, capability to apply special ..
  33. CORE--HISTOPATHOLOGY AND EXPERIMENTAL ANIMAL FACILITY
    Maarten Bosland; Fiscal Year: 1999
    ..cryostat sectioning, preparation of slides for immunohistochemistry and in situ hybridization and PCR, plastic embedding and slide preparation, routine conventional and (immuno)histochemical staining, capability to apply special ..
  34. CORE--MORPHOLOGY
    MARY BUNGE; Fiscal Year: 2000
    ..The Core will process tissues for plastic embedding for both light and electron microscopic analysis...
  35. CORE--MORPHOLOGY
    MARY BUNGE; Fiscal Year: 2001
    ..The Core will process tissues for plastic embedding for both light and electron microscopic analysis...
  36. CORE GRANT FOR VISION RESEARCH
    JONATHAN PETRASH; Fiscal Year: 1999
    ..3. An immunopathology module will provide technical support and expertise in paraffin and plastic embedding and sectioning, will provide expertise in immunohistology and immunohistochemistry, will assist in ..
  37. CORE--MORPHOLOGY
    MARY BUNGE; Fiscal Year: 2002
    ..The Core will process tissues for plastic embedding for both light and electron microscopic analysis...
  38. CORE--MORPHOLOGY
    MARY BUNGE; Fiscal Year: 2002
    ..The Core will process tissues for plastic embedding for both light and electron microscopic analysis...
  39. CORE--MORPHOLOGY
    MARY BUNGE; Fiscal Year: 2002
    ..The Core will process tissues for plastic embedding for both light and electron microscopic analysis...
  40. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2004
    ..Our goal is to provide information on possible mechanisms of noise damage in the cochlea as a basis for developing scientifically sound strategies for reducing noise-induced hearing loss in humans. ..
  41. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2002
    ..Our goal is to provide information on possible mechanisms of noise damage in the cochlea as a basis for developing scientifically sound strategies for reducing noise-induced hearing loss in humans. ..
  42. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2003
    ..Our goal is to provide information on possible mechanisms of noise damage in the cochlea as a basis for developing scientifically sound strategies for reducing noise-induced hearing loss in humans. ..
  43. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2008
    ..Our results will provide information on mechanisms of damage from moderate noise so that drugs that will prevent or minimize noise-induced hair-cell death can be developed. ..
  44. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2001
    ..Our goal is to provide information on possible mechanisms of noise damage in the cochlea as a basis for developing scientifically sound strategies for reducing noise-induced hearing loss in humans. ..
  45. Adverse Effects of Noise on Hearing: Basic Mechanisms
    BARBARA BOHNE; Fiscal Year: 2009
    ..Our results will provide information on mechanisms of damage from moderate noise so that drugs that will prevent or minimize noise-induced hair-cell death can be developed. ..