corrosion casting

..This process occurs without disrupting organ function. Improvements in our understanding of the process should enable the development of novel pro- and anti-angiogenic therapeutic treatments...

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..is limited, the in vivo studies are complemented by a powerful analysis of the vasculature using vascular corrosion casting. Both techniques revealed age-dependent blood flow alterations and cerebrovascular abnormalities in these ..

..Capillary regression hits F at any stage and characterizes F atresia...

Vascular corrosion casting has been established as a method to reconstruct the three-dimensional (3D) structure of blood vessels of organs and tissues...

..The methods described have been used to cast the mouse uteroplacental vasculature from 5.5-18.5 d gestation and the fetoplacental vasculature from 12.5 d gestation to term...

..The differential angiogenic and degenerative response of theca interna capillaries may reflect differences in the microenvironment of the follicles, which in turn determine the fate of the follicles (continued growth versus atresia)...

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..Differential quantitative outcome of IBR in arteries and veins, and the experimental induction of IBR strongly suggest that hemodynamic factors can instruct embryonic vascular remodeling toward optimality...

..Vascularization of tissues, organs, and tumors rely heavily on both mechanisms; neglecting one or the other would obscure our understanding of the angiogenesis process...

..This may be probably related to the significant increase of the ovarian blood flow necessary for the maintenance of CL endocrine functions during pregnancy...

..The present observations indicate that follicular vascular baskets of diverse sizes and architecture in various developmental stages support the gradual increase of follicular blood flow during follicle growth in the pig...

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..Use of the term "open circulatory system" within arthropods is re-emphasized, as it refers to the general organization of the body cavity (i.e. mixocoely) rather than to the complexity of the circulatory system...

..The vasculature and structure of these lymph nodes was described using a combination of vascular corrosion casting, ink injection, and light and electron microscopy...

The vascular corrosion casting technique produces a replica of vascular beds of normal or pathological tissues...

..The objective of this study was to determine whether there are also aging changes in the microvascular architecture and vascular dispersion of the liver that might influence liver function...

..We examined the possibility that ischaemic preconditioning might preserve the integrity of the coronary microcirculation following ischaemia and reperfusion...

..Aging changes of capillary density and arterial morphology of the spinal cord may be important contributory factors in vascular myelopathy...

..The present study suggested that the risk of brain edema or hemorrhagic infarction increased beyond 3 h of MCA occlusion, and vasospasm of the arterioles might participate in stroke pathophysiology...

..In addition, we confirmed the close correspondence between cellular ultrastructural detail (pores, corrugations of cellular membrane, perivascular cell branches) and the impressions left on the resin visible only at high magnifications...

..gilts 72 h after equine chorionic gonadotropin (eCG) (1,250 IU) treatment, using combined vascular corrosion casting and scanning electron microscopy...

..In the nail bed, the vessels were arranged in many longitudinal trabeculae parallel to the major axis of the digit. In the root of the nail, we found many columnar vessels characterized by multiple angiogenic buttons on their surface...

..Perioperative CO inhalation at a low concentration resulted in protection against ischemia/reperfusion injury to intestinal grafts with prolonged cold preservation...

..Therefore, the technique may be useful for macroscopic studies of large specimens, retaining sufficiently fine details...

..These findings are contrary to the thesis that defends the existence of a physiologically closed and morphologically open circulation in the dog spleen. Lymphatic vessels in the spleen of the dog are also demonstrated...

..CNVs in rats treated with FR118487 were less well developed than in the controls. CONCLUSION: FR118487 inhibits the development of experimental CNV induced by photocoagulation in pigmented rats...

..The aim of this study is to investigate the ultra structural difference of placentas in IUGR fetuses that were all found to have abnormal umbilical artery Doppler waveforms...

..We suggest that this kind of lesion and the secondary functional injury to the blood-brain barrier (BBB) could play an important role in the development of the secondary lesions that these patients show in the subacute phase...

..This difference may influence pathological changes in vascular lesions in these respective areas...

..Angiogenic remodeling of the pododermal angioarchitecture was also the connectional reparative principle in pathologically altered claws. Related research perspectives for prophylaxis and therapy of claw diseases are discussed...

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..this reason, we have studied the terminal hepatic artery branches in the rat by using special microvascular corrosion casting procedure which makes possible to better follow the hepatic artery terminal branches...

..These results suggest that these previously unappreciated inflammation-responsive precapillary constrictors regulate capillary recruitment in the mouse ear microcirculation...

..Blood flowing across the vascular endothelium creates wall shear stress, dependent on velocity of flow and vessel geometry, that tends to disrupt lymphocyte-endothelial cell adhesion...

..The present study uses vascular corrosion casting and scanning electron microscopy to describe the three-dimensional anatomy of the microvasculature of the ..

..The ultimate goal of this series of studies is the application of cells onto a prevascularised matrix, with the ambition to enhance cell survival after transplantation in vivo...

..Quantitative data are discussed both in respect to methodical pitfalls and the optimality principles possibly underlying the design of vascular bifurcations/mergings in selected brain areas of a nonteleost primitive actinopterygian fish...

..Swainsonine caused a great distortion to the vasculature at 18 weeks. The effects of this compound on the vascular development lend credibility to its potential as an anticancer agent...

..We developed a method based on modified vascular corrosion casting (VCC), scanning electron microscopy (SEM), and desktop and synchrotron radiation microCT (SRmicroCT) ..

..The reported microvascular organization and its adaptation to the epiphyseal growth represent the morphological basis for understanding the reciprocal interaction among the different tissues in developing and adult rat epiphysis...

..Therefore, the morphology of the vasculature distinctly reflects the functional status of this primary lymphoid organ during its lifespan...

..Static ex vivo modalities still prevail, however, the future will be dominated by intravital high-resolution techniques...

The three-dimensional microvascular structure of many organs can be adequately investigated only using the corrosion casting technique...

..Thus, we investigated bone remodeling in the healing process after mucoperiosteal flap surgery, focusing our attention on the microcirculation...

..To investigate the role of the periosteal vascular plexus in the healing process, we used 3-dimensional (3-D) and ultrastructural monitoring of the angiogenic process after elevation of the mucoperiosteal flap...

..The implantation of vascular carriers into given growth factor-loaded matrix volumes may eventually allow efficient generation of axially vascularized, tissue-engineered composites...

..The utility of vessel painting in morphometry was assessed using morphometric comparisons with corrosion casting and 2-dimensional and 3-dimensional scanning electron microscopy...

..We studied the effect exerted on the PDL vascular plexus during restoration of the circulatory tract...

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..24 +/- 4.66 degrees in the olfactory bulb. Finally, the branching angles of the venules and veins range from 67.84 +/- 6.83 degrees in the tegmentum of the mesencephalon to 92.30 +/- 6.35 degrees in the optic tectum...

..This venous trunk had not been described in literature till now and does not have a name in anatomical nomenclature. The authors showed the relationship of the pineal gland to adjacent venous vessels...

..These findings indicate that hypertensive choroidopathy is as important as retinopathy...

..The results of the present study will be useful in the interpretation of the changes seen in hypertensive, diabetic and aged choroids...

..The nuclei of endothelial cell cores could be seen on the vessel surfaces. Collagen fibers type I and III were noted in the connective tissue and in the fibrous layer of vessels...

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..They were sparse and showed a few primordial pores. In addition, they began to make contacts with primordial podocytes. At the stage of 40-60 cm CRT, the renal microcirculation showed a complex and almost completely organized morphology...

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..Each region of the olfactory organ had characteristic three-dimensional vascular patterns that were related to their cellular architecture...

..These venules were collected into the large vein at the bottom of the tonsil. The blood vascular architecture of the musk shrew tonsil is basically the same as those of other mucosa-associated lymphoid tissues in mammals...

..This dramatic event is followed by almost complete recovery of glomerular architecture. However, the precise cellular mechanisms of revascularization are not fully understood...

..The role of Rho proteins in endothelial cell shape change in situ in the myocardial capillary bed has to date not been investigated...

..To demonstrate the vascular anatomy of the normal and undescended testes, emphasizing the surgical importance of the anastomotic channels between the testicular and deferential arteries in the undescended testis...

..and fetal microvasculature was studied in ewes at days 50, 90 and 130 of gestation using microvascular corrosion casting and scanning electron microscopy...

..Sound king crabs, Lopholithodes mandtii, and brown box crabs, Lopholithodes foraminatus, were mapped using corrosion casting techniques. Both species have a similar external morphology and a very similar cardiovascular system...

..Samples were then injected with India ink or methylmetacrylate Mercox resin for microdissection and corrosion casting for scanning electron microscopy...

..of three euphausiacean species based on semi-thin sections and micro computer tomography in combination with corrosion casting. We were also able to study for the first time representatives of Bentheuphausia amblyops, the suggested ..

b>Corrosion casting and immunohistochemical staining with anti-alpha smooth muscle actin and anti-CD34 was utilized to demonstrate the capillary plexus and venous system in control and malformed mouse hearts.

..Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed to date...

The cardiovascular system of majid crabs was mapped using corrosion casting techniques...

..We applied corrosion casting (CC) technique to the toes of a patient affected by Type 2 diabetes, who underwent surgery for explantation ..

..We used a corrosion casting technique applied to nude mice previously transplanted subcutaneously with human modified neoplastic Kaposi ..

..network of normal colon mucosa, adenomas and invasive carcinomas by means of quantitative microvascular corrosion casting. Fresh hemicolectomy specimens from 20 patients undergoing cancer or polyposis coli surgery were used for ..

..To understand the etiology of this disorder, we evaluated the cerebral vasculature of endoglin heterozygous (Eng+/-) mice, which represent the only animal model of HHT1...

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Microvascular corrosion casting was used to assess the effects of thrombin and D609, a phospholipase C inhibitor, on the vascularity of the chick embryo chorioallantoic membrane (CAM)...

..Three-dimensional microvascular corrosion casting and transmission electron microscopy showed further structural differences between xenografts...

..b>Corrosion casting and scanning electron microscopy of the mouse colon demonstrated highly branched bridging vessels that ..

..Nor has endothelial visualization been facilitated. Vascular corrosion casting techniques are promising in that regard, and were applied in an extended lateral thoracoabdominal ..

..The geometry of six saphenous vein CABGs was replicated by post-mortem infusion of Batson's #17 anatomical corrosion casting compound...

Vascular architecture of the human pancreas was investigated by corrosion casting combined with scanning electron microscopy in fetuses aged 20 and 25 gestational weeks...

..modified processing techniques for both transmission and scanning electron microscopy including micro-corrosion casting of cultured cells were established...

Vascular corrosion casting provides a permanent three-dimensional record of the deeper vasculature of the anterior segment whereas fluorescein angiography allows clinical examination of superficial vessels...

..an analysis of serial histological sectioning, ultrathin sections, microvascular perfusion with plastics and corrosion casting, X-ray microcomputed tomography, in vivo imaging including analysis of transgenic mice expressing GFP-..

..To quantify tissue remodeling induced by static and cyclical application of tensional forces in a living perfused tissue...

..microscopy demonstrated an immature sinusoidal architecture in the liver and persistent hyaloid arteries in the eyes of adult Ah null mice, whereas corrosion casting experiments described aberrations in kidney vascular patterns.

..immunohistochemistry, vascular perfusion by intravital Lycopersicon esculentum lectin perfusion and vascular corrosion casting. Micro-CT scanning found a similar level of vascularisation within the VEGF treated scaffolds to that ..

To construct in vitro models of normal uterine arterial vascular network through vascular corrosion casting and explore their characteristics and clinical significances.

..b>Corrosion casting techniques were refined and used to map the circulatory system of a variety of crab species (order: Decapoda;..

..normal tissue, transitional mucosa and adenocarcinoma of the human colon was investigated with microvascular corrosion casting (MVCC) combined with scanning electron microscopy (SEM)...

..To quantitatively assess microvascular dimensions in the eyes of neonatal wild-type and VEGF(120)-tg mice, using a novel combination of techniques which permit three-dimensional (3D) image reconstruction...

..In an additional study, animals were treated at day 19 and quantitative three-dimensional microvascular corrosion casting was performed to enable detailed assessment of the tumor vascular architecture...

..study, animals were treated according to the same regimen and quantitative three-dimensional microvascular corrosion casting was performed to enable detailed assessment of the tumor vascular architecture...

..Here we used vascular corrosion casting and hierarchical micro-computed tomography combined with a new network analysis tool to characterize the ..

..In this study we focused on the chronological changes in VEGF concentration and flap perfusion in order to optimise the duration of surgical delay...

..Preliminary results showed a marked increase in coronary blood flow associated with exposure to environmental hypoxia...

..Using the method of corrosion casting visualized with scanning electron microscopy, a single dose of CYT997 (7.5 mg/kg i.p...

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..Previously, the PI has detailed the microvascular anatomy (methylmethacrylate corrosion casting) of the anterior optic nerve of non-primate mammals, non-human primates and humans...

..Previously, the PI has detailed the microvascular anatomy (methylmethacrylate corrosion casting) of the anterior optic nerve of non-primate mammals, non-human primates and humans...

..b>Corrosion casting and 3-dimensional scanning electron microscopy will be used to visualize and quantify the structural ..

..Moreover, one of the principal techniques allowing the collection of 3D quantitative vascular data, corrosion casting, destroys surrounding tissue, eliminating the possibility of simultaneous probing for molecules of interest...

..Demonstrated by corrosion casting and 3-dimensional scanning electron microscopy (SEM), the pillars form within 7 days after chemical exposure...

..Demonstrated by corrosion casting and 3-dimensional scanning electron microscopy (SEM), the pillars form within 7 days after chemical exposure...

..The third aim will focus on the use of 3D tissue sections and corrosion casting/scanning electron microscopy to make detailed observations of the inflammatory microcirculation...

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..This will be facilitated by the investigator's longstanding and close collaboration with experimental physiologists. ..

..This will be facilitated by well-established and active collaborations with experimental physiologists. ..

..A better knowledge of the hepatic pathophysiology of alcohol bringing also should result. ..

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..This will be facilitated by well-established and active collaborations with experimental physiologists. ..

..The results should provide novel, new information about the aging liver. ..

..Predictions will be compared with experimentally measured distributions in the same vascular networks. ..

..3. EFFICACY. The new formulations will also be evaluated for activity against sperm, chlamydia and HSV utilizing in vitro assays and the mouse model. ..

..Predictions will be compared with experimentally measured distributions in the same vascular networks. ..

..This will be facilitated by well-established and active collaborations with experimental physiologists. ..

..Particular emphasis will be given to comparisons between model predictions and corresponding experimental observations, taking advantage of well- established consultative and collaborative arrangements with experimental hemorheologists...

..Thus, impaired flow in some diseases may result principally from the cells' reduced ability to traverse non-uniform pathways. The proposed research will provide quantitative predictions about these aspects of microvascular blood flow...

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..This will be facilitated by well-established and active collaborations with experimental hemorheologists and physiologists. ..