flow cytometry


Summary: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

Top Publications

  1. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
  2. pmc Prospective identification of tumorigenic breast cancer cells
    Muhammad Al-Hajj
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 100:3983-8. 2003
  3. ncbi Multilineage cells from human adipose tissue: implications for cell-based therapies
    P A Zuk
    Laboratory for Regenerative Bioengineering and Repair, UCLA School of Medicine, Los Angeles, California, USA
    Tissue Eng 7:211-28. 2001
  4. pmc Obesity is associated with macrophage accumulation in adipose tissue
    Stuart P Weisberg
    Division of Moleuclar Genetics, Department of Pediatrics, Columbia University, New York, New York 10032, USA
    J Clin Invest 112:1796-808. 2003
  5. ncbi A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
    I Nicoletti
    Istituto di Clinica Medica 1, Perugia University School of Medicine, Italy
    J Immunol Methods 139:271-9. 1991
  6. ncbi 'Green mice' as a source of ubiquitous green cells
    M Okabe
    Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEBS Lett 407:313-9. 1997
  7. ncbi IL-6 programs T(H)-17 cell differentiation by promoting sequential engagement of the IL-21 and IL-23 pathways
    Liang Zhou
    The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, New York 10016, USA
    Nat Immunol 8:967-74. 2007
  8. pmc Phenotypic and functional features of human Th17 cells
    Francesco Annunziato
    Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders DENOThe, University of Florence, Florence 50134, Italy
    J Exp Med 204:1849-61. 2007
  9. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
  10. pmc Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells
    Angela M Thornton
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 184:3433-41. 2010

Detail Information

Publications360 found, 100 shown here

  1. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
    ..These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential...
  2. pmc Prospective identification of tumorigenic breast cancer cells
    Muhammad Al-Hajj
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 100:3983-8. 2003
    ..Furthermore, because these cells drive tumor development, strategies designed to target this population may lead to more effective therapies...
  3. ncbi Multilineage cells from human adipose tissue: implications for cell-based therapies
    P A Zuk
    Laboratory for Regenerative Bioengineering and Repair, UCLA School of Medicine, Los Angeles, California, USA
    Tissue Eng 7:211-28. 2001
    ..Immunofluorescence and flow cytometry show that the majority of PLA cells are of mesodermal or mesenchymal origin with low levels of contaminating ..
  4. pmc Obesity is associated with macrophage accumulation in adipose tissue
    Stuart P Weisberg
    Division of Moleuclar Genetics, Department of Pediatrics, Columbia University, New York, New York 10032, USA
    J Clin Invest 112:1796-808. 2003
    ..Adipose tissue macrophage numbers increase in obesity and participate in inflammatory pathways that are activated in adipose tissues of obese individuals...
  5. ncbi A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
    I Nicoletti
    Istituto di Clinica Medica 1, Perugia University School of Medicine, Italy
    J Immunol Methods 139:271-9. 1991
    ..This new rapid, simple and reproducible method should prove useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes...
  6. ncbi 'Green mice' as a source of ubiquitous green cells
    M Okabe
    Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    FEBS Lett 407:313-9. 1997
    ..The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments...
  7. ncbi IL-6 programs T(H)-17 cell differentiation by promoting sequential engagement of the IL-21 and IL-23 pathways
    Liang Zhou
    The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, New York 10016, USA
    Nat Immunol 8:967-74. 2007
    ..IL-6 therefore orchestrates a series of 'downstream' cytokine-dependent signaling pathways that, in concert with TGF-beta, amplify RORgammat-dependent differentiation of T(H)-17 cells...
  8. pmc Phenotypic and functional features of human Th17 cells
    Francesco Annunziato
    Center for Research, Transfer and High Education on Chronic, Inflammatory, Degenerative and Neoplastic Disorders DENOThe, University of Florence, Florence 50134, Italy
    J Exp Med 204:1849-61. 2007
  9. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
    ..Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression...
  10. pmc Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells
    Angela M Thornton
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 184:3433-41. 2010
    ..Collectively, these results demonstrate that Helios is potentially a specific marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3(+) Treg cells are generated extrathymically...
  11. pmc Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature
    Emmanuelle Charafe-Jauffret
    Centre de Recherche en Cancerologie de Marseille, Laboratoire d Oncologie Moleculaire, UMR891 Inserm Institut Paoli Calmettes, Universite de la Mediterranee, Marseille, France
    Cancer Res 69:1302-13. 2009
  12. ncbi CD4+CD25+ regulatory T cells control Leishmania major persistence and immunity
    Yasmine Belkaid
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 420:502-7. 2002
  13. ncbi Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells
    Maria M Ho
    Department of Cancer Genetics and Developmental Biology, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
    Cancer Res 67:4827-33. 2007
    ..In this study, we used flow cytometry and Hoechst 33342 dye efflux assay to isolate and characterize SP cells from six human lung cancer cell lines (..
  14. ncbi Development, cytokine profile and function of human interleukin 17-producing helper T cells
    Nicholas J Wilson
    Department of Discovery Research, Schering Plough Biopharma Formerly DNAX Research, Palo Alto, California 94304 1104, USA
    Nat Immunol 8:950-7. 2007
    ..Our data collectively indicate that human and mouse T(H)-17 cells require distinct factors during differentiation and that human T(H)-17 cells may regulate innate immunity in epithelial cells...
  15. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
    ..These findings offer an important new tool for the study of normal and malignant breast stem cells and facilitate the clinical application of stem cell concepts...
  16. ncbi IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge
    Shabaana A Khader
    Trudeau Institute, Saranac Lake, New York 12983, USA
    Nat Immunol 8:369-77. 2007
  17. pmc Highly tumorigenic lung cancer CD133+ cells display stem-like features and are spared by cisplatin treatment
    Giulia Bertolini
    Molecular Cytogenetics Unit, Department of Experimental Oncology, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico, Istituto Nazionale dei Tumori, 20133 Milan, Italy
    Proc Natl Acad Sci U S A 106:16281-6. 2009
    ..The molecular features of these cells may provide the rationale for more specific therapeutic targeting and the definition of predictive factors in clinical management of this lethal disease...
  18. doi T-cell quality in memory and protection: implications for vaccine design
    Robert A Seder
    Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Rev Immunol 8:247-58. 2008
    T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T ..
  19. ncbi The response of CD24(-/low)/CD44+ breast cancer-initiating cells to radiation
    Tiffany M Phillips
    Department of Radiation Oncology, David Geffen School of Medicine at UCLA, 10833 Le Conte Ave, Los Angeles, CA 90095 1714, USA
    J Natl Cancer Inst 98:1777-85. 2006
    ..Cancer-initiating cells can now be prospectively isolated from breast cancer cell lines and tumor samples and propagated as mammospheres in vitro under serum-free conditions...
  20. ncbi Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells
    Eva V Acosta-Rodriguez
    Institute for Research in Biomedicine, CH 6500 Bellinzona, Switzerland
    Nat Immunol 8:942-9. 2007
  21. ncbi Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors
    Jalees Rehman
    Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, USA
    Circulation 107:1164-9. 2003
    ..It is not known whether the proangiogenic effects are a result of such events as endothelial differentiation and subsequent proliferation of EPCs or secondary to secretion of angiogenic growth factors...
  22. pmc SPICE: exploration and analysis of post-cytometric complex multivariate datasets
    Mario Roederer
    Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA
    Cytometry A 79:167-74. 2011
    Polychromatic flow cytometry results in complex, multivariate datasets...
  23. ncbi Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
  24. pmc Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata
    Filip K Swirski
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    J Clin Invest 117:195-205. 2007
    ..Conversely, the number of Ly-6C(lo) cells remained unaffected. Thus, we believe that Ly-6C(hi) monocytes represent a newly recognized component of the inflammatory response in experimental atherosclerosis...
  25. pmc Generation of breast cancer stem cells through epithelial-mesenchymal transition
    Anne Pierre Morel
    Centre Leon Berard, Lyon, France
    PLoS ONE 3:e2888. 2008
    ..The acquisition of these stem and tumorigenic characters is driven by EMT induction...
  26. ncbi Noise in protein expression scales with natural protein abundance
    Arren Bar-Even
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Genet 38:636-43. 2006
  27. ncbi A tumorigenic subpopulation with stem cell properties in melanomas
    Dong Fang
    Program of Molecular and Cellular Oncogenesis, The Wistar Institute and Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, PA 19104, USA
    Cancer Res 65:9328-37. 2005
    ..Based on these findings, we propose that melanomas can contain a subpopulation of stem cells that contribute to heterogeneity and tumorigenesis. Targeting this population may lead to effective treatments for melanomas...
  28. ncbi Human endothelial progenitor cells from type II diabetics exhibit impaired proliferation, adhesion, and incorporation into vascular structures
    Oren M Tepper
    Laboratory of Microvascular Research and Vascular Tissue Engineering, Institute of Reconstructive Plastic Surgery, New York University Medical Center, New York, NY 10016, USA
    Circulation 106:2781-6. 2002
    ..Because diabetic complications occur in conditions in which EPC contributions have been demonstrated, EPC dysfunction may be important in their pathophysiology...
  29. ncbi Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response
    Cord Sunderkotter
    Institute of Experimental Dermatology and Department of Dermatology, University of Munster, Munster, Germany
    J Immunol 172:4410-7. 2004
    ..Taken together, these data identify distinct subpopulations of mouse blood monocytes that differ in maturation stage and capacity to become recruited to inflammatory sites...
  30. pmc Fate mapping of IL-17-producing T cells in inflammatory responses
    Keiji Hirota
    Division of Molecular Immunology, Medical Research Council National Institute for Medical Research, Mill Hill, London, UK
    Nat Immunol 12:255-63. 2011
    ..Thus, this model allows the actual functional fate of effector T cells to be related to T(H)17 developmental origin regardless of IL-17 expression...
  31. pmc A distinct "side population" of cells with high drug efflux capacity in human tumor cells
    C Hirschmann-Jax
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children s Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:14228-33. 2004
  32. pmc CD4(+)CD25(-)Foxp3(-) Th1 cells are the source of IL-10-mediated immune suppression in chronic cutaneous leishmaniasis
    Charles F Anderson
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:285-97. 2007
  33. doi A hierarchy of self-renewing tumor-initiating cell types in glioblastoma
    Ruihuan Chen
    Department of Tumor Biology and Angiogenesis, Genentech Inc, San Francisco, CA 94080, USA
    Cancer Cell 17:362-75. 2010
  34. ncbi The inhibitory cytokine IL-35 contributes to regulatory T-cell function
    Lauren W Collison
    Department of Immunology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 450:566-9. 2007
    ..Taken together, these data identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity...
  35. ncbi Identification of a factor that links apoptotic cells to phagocytes
    Rikinari Hanayama
    Department of Genetics, Osaka University Medical School, 2 2 Yamada oka, Suita, Osaka, 565 0871, Japan
    Nature 417:182-7. 2002
    ..These results indicate that MFG-E8 secreted from activated macrophages binds to apoptotic cells, and brings them to phagocytes for engulfment...
  36. ncbi Microglia in the adult brain arise from Ly-6ChiCCR2+ monocytes only under defined host conditions
    Alexander Mildner
    Institute of Neuropathology, Georg August University, Gottingen, Germany
    Nat Neurosci 10:1544-53. 2007
    ..Our results identify Ly-6C(hi)CCR2(+) monocytes as direct precursors of microglia in the adult brain and establish the importance of local factors in the adult CNS for microglia engraftment...
  37. pmc Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus
    Emily C Baechler
    Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:2610-5. 2003
    ..These results provide insights into the genetic pathways underlying SLE, and identify a subgroup of patients who may benefit from therapies targeting the IFN pathway...
  38. pmc Foxp3-dependent microRNA155 confers competitive fitness to regulatory T cells by targeting SOCS1 protein
    Li Fan Lu
    Department of Immunology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Immunity 30:80-91. 2009
  39. ncbi Effector memory T cells, early metastasis, and survival in colorectal cancer
    Franck Pages
    INSERM UNITE 255, René Descartes Faculté de Médecine, Assistance Publique Hopitaux de Paris, Cordeliers Biomedical Research Center, University Paris 6, Paris, France
    N Engl J Med 353:2654-66. 2005
    ..The role of tumor-infiltrating immune cells in the early metastatic invasion of colorectal cancer is unknown...
  40. pmc Efficient targeting of protein antigen to the dendritic cell receptor DEC-205 in the steady state leads to antigen presentation on major histocompatibility complex class I products and peripheral CD8+ T cell tolerance
    Laura Bonifaz
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021, USA
    J Exp Med 196:1627-38. 2002
    ..Therefore, DEC-205 provides an efficient receptor-based mechanism for DCs to process proteins for MHC class I presentation in vivo, leading to tolerance in the steady state and immunity after DC maturation...
  41. pmc Regulatory mechanisms for adipose tissue M1 and M2 macrophages in diet-induced obese mice
    Shiho Fujisaka
    First Department of Internal Medicine, Graduate School of Medicine and Pharmaceutical Science for Research, Tokyo, Japan
    Diabetes 58:2574-82. 2009
    ..To characterize the phenotypic changes of adipose tissue macrophages (ATMs) under different conditions of insulin sensitivity...
  42. ncbi Transcriptional regulation of nanog by OCT4 and SOX2
    David J Rodda
    Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    J Biol Chem 280:24731-7. 2005
    ..These studies extend the understanding of the pluripotent genetic regulatory network within which the Sox2-Oct4 complex are at the top of the regulatory hierarchy...
  43. ncbi Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells
    L Patrawala
    Department of Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, 78957, USA
    Oncogene 25:1696-708. 2006
    ..Altogether, our results suggest that the CD44(+) PCa cell population is enriched in tumorigenic and metastatic progenitor cells...
  44. ncbi HLA expression and immunologic properties of differentiated and undifferentiated mesenchymal stem cells
    Katarina Le Blanc
    Division of Clinical Immunology, Centre for Allogeneic Stem Cell Transplantation, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
    Exp Hematol 31:890-6. 2003
    ..Mesenchymal stem cells (MSC) do not elicit alloreactive lymphocyte responses due to immune modulations. We investigated the immunologic properties of MSC after differentiation along three lineages: bone, cartilage, and adipose...
  45. pmc Molecular antagonism and plasticity of regulatory and inflammatory T cell programs
    Xuexian O Yang
    Department of Immunology, M D Anderson Cancer Center, Houston, TX 77030, USA
    Immunity 29:44-56. 2008
    ..STAT3 regulated Foxp3 downregulation, whereas STAT3, RORgamma, and ROR* were required for IL-17 expression in Treg cells. Our data demonstrate molecular antagonism and plasticity of Treg and Th17 cell programs...
  46. pmc Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes
    Carey N Lumeng
    Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA
    Diabetes 57:3239-46. 2008
    ..To establish the mechanism of the phenotypic switch of adipose tissue macrophages (ATMs) from an alternatively activated (M2a) to a classically activated (M1) phenotype with obesity...
  47. pmc Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell Stem Cell 3:391-401. 2008
  48. doi C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE
    Andrea Reboldi
    Institute for Research in Biomedicine, Bellinzona, Switzerland
    Nat Immunol 10:514-23. 2009
    ..Our results identify distinct molecular requirements and ports of lymphocyte entry into uninflamed versus inflamed CNS and suggest that the CCR6-CCL20 axis in the choroid plexus controls immune surveillance of the CNS...
  49. ncbi Direct isolation of satellite cells for skeletal muscle regeneration
    Didier Montarras
    CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France
    Science 309:2064-7. 2005
    ..mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- ..
  50. pmc Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity
    Xuyu Zhou
    Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA
    J Exp Med 205:1983-91. 2008
    ..These results support a central role for miRNAs in maintaining the stability of differentiated T reg cell function in vivo and homeostasis of the adaptive immune system...
  51. ncbi Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches
    Tatsuki Sugiyama
    Department of Medical Systems Control, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Immunity 25:977-88. 2006
    ..CXCL12-CXCR4 signaling plays an essential role in maintaining the quiescent HSC pool, and CAR cells appear to be a key component of HSC niches, including both vascular and endosteal niches in adult bone marrow...
  52. pmc Generation of T follicular helper cells is mediated by interleukin-21 but independent of T helper 1, 2, or 17 cell lineages
    Roza I Nurieva
    Department of Immunology, University of Texas MD Anderson Cancer Center and Graduate School of Biomedical Sciences, Houston, TX 77030, USA
    Immunity 29:138-49. 2008
    ..This study thus demonstrates that Tfh is a distinct Th cell lineage...
  53. ncbi Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization
    Jun Ichi Yamaguchi
    Division of Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 107:1322-8. 2003
    ..Given the close relationship between hematopoietic stem cells and endothelial progenitor cells (EPCs), we investigated the effect of SDF-1 on EPC-mediated vasculogenesis...
  54. pmc Late developmental plasticity in the T helper 17 lineage
    Yun Kyung Lee
    Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Immunity 30:92-107. 2009
    ..These results support a model of late Th17 developmental plasticity with implications for autoimmunity and host defense...
  55. doi Snail and slug mediate radioresistance and chemoresistance by antagonizing p53-mediated apoptosis and acquiring a stem-like phenotype in ovarian cancer cells
    Nawneet K Kurrey
    National Centre for Cell Science, NCCS Complex, Pune University Campus, Pune, India
    Stem Cells 27:2059-68. 2009
  56. ncbi Neural stem and progenitor cells in nestin-GFP transgenic mice
    John L Mignone
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Comp Neurol 469:311-24. 2004
  57. pmc Increased circulating myeloid-derived suppressor cells correlate with clinical cancer stage, metastatic tumor burden, and doxorubicin-cyclophosphamide chemotherapy
    C Marcela Diaz-Montero
    Department of Surgery, Medical University of South Carolina, Hollings Cancer Center, Charleston, SC, USA
    Cancer Immunol Immunother 58:49-59. 2009
    ..This information must be incorporated into the design of future trials exploring immune-based therapeutic strategies. Pharmacologic modulation of MDSC should also be tested in future clinical trials...
  58. ncbi Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow
    C Campagnoli
    Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, London, United Kingdom
    Blood 98:2396-402. 2001
    ..MSCs similar to those derived from adult bone marrow, fetal liver, and fetal bone marrow circulate in first-trimester human blood and may provide novel targets for in utero cellular and gene therapy...
  59. doi A perivascular origin for mesenchymal stem cells in multiple human organs
    Mihaela Crisan
    Department of Pediatrics, Stem Cell Research Center, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Cell Stem Cell 3:301-13. 2008
    ..Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells...
  60. ncbi Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions
    H Jonuleit
    Department of Dermatology, University of Mainz, Germany
    Eur J Immunol 27:3135-42. 1997
    ..TNF-alpha/IL-1, IL-6 + PGE2-cultured DC seem to be optimal for generation of IFN-gamma-producing CD4/CD8+ T cells...
  61. ncbi Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta
    Pieternella S In 't Anker
    Department of Obstetrics, Lieden University Medical Center, Leiden, The Netherlands
    Stem Cells 22:1338-45. 2004
    ..Decidua basalis and decidua parietalis are sources for maternal MSCs. The expansion potency from both fetal and maternal placenta-derived MSCs was higher compared with adult bone marrow-derived MSCs...
  62. pmc Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation during colon tumorigenesis
    Emina H Huang
    Department of Surgery, University of Florida, Gainesville, Florida, USA
    Cancer Res 69:3382-9. 2009
    ..These findings also support our original hypothesis, derived previously from mathematical modeling of crypt dynamics, that progressive colonic SC overpopulation occurs during colon tumorigenesis and drives CRC development...
  63. pmc Increase in TNF-alpha and inducible nitric oxide synthase-expressing dendritic cells in psoriasis and reduction with efalizumab (anti-CD11a)
    Michelle A Lowes
    Laboratory for Investigative Dermatology and Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:19057-62. 2005
  64. ncbi Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants
    David Traver
    Children s Hospital Boston and the Howard Hughes Medical Institute, 320 Longwood Avenue, Enders 720, Boston, Massachusetts 02115, USA
    Nat Immunol 4:1238-46. 2003
    ..Each major blood lineage can be isolated by flow cytometry, and with these lineal profiles, defects in zebrafish blood mutants can be quantified...
  65. ncbi Identification of clonogenic common lymphoid progenitors in mouse bone marrow
    M Kondo
    Department of Pathology, Stanford University School of Medicine, California 94305, USA
    Cell 91:661-72. 1997
    ..A single Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) cell could generate at least both T and B cells. These data provide direct evidence for the existence of common lymphoid progenitors in sites of early hematopoiesis...
  66. pmc A human memory T cell subset with stem cell-like properties
    Luca Gattinoni
    Center for Cancer Research, National Cancer Institute, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Med 17:1290-7. 2011
    ..The identification of a human stem cell-like memory T cell population is of direct relevance to the design of vaccines and T cell therapies...
  67. ncbi Isolation and expansion of adult cardiac stem cells from human and murine heart
    Elisa Messina
    Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy
    Circ Res 95:911-21. 2004
  68. doi Multifunctional CD4(+) T cells correlate with active Mycobacterium tuberculosis infection
    Nadia Caccamo
    Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, University of Palermo, Palermo, Italy
    Eur J Immunol 40:2211-20. 2010
    ..Using multiparametric flow cytometry, we have evaluated the distribution of seven distinct functional states (IFN-γ/IL-2/TNF-α triple expressors, ..
  69. ncbi Side population purified from hepatocellular carcinoma cells harbors cancer stem cell-like properties
    Tetsuhiro Chiba
    Department of Regenerative Medicine, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan
    Hepatology 44:240-51. 2006
    ..In conclusion, we propose that a minority population, detected as SP cells in HCC cells, possess extreme tumorigenic potential and provide heterogeneity to the cancer stem cell system characterized by distinct hierarchy...
  70. doi Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses
    Harumichi Ishigame
    Center for Experimental Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Immunity 30:108-19. 2009
    ..These observations indicate that IL-17A and IL-17F have overlapping yet distinct roles in host immune and defense mechanisms...
  71. doi Prolonged interleukin-2Ralpha expression on virus-specific CD8+ T cells favors terminal-effector differentiation in vivo
    Vandana Kalia
    Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
    Immunity 32:91-103. 2010
    ..Consistent with this, sustained IL-2 receptor signaling during expansion drove terminal-effector differentiation. These data support the hypothesis that prolonged IL-2 signals during priming promote terminal-effector differentiation...
  72. pmc Severe CD4+ T-cell depletion in gut lymphoid tissue during primary human immunodeficiency virus type 1 infection and substantial delay in restoration following highly active antiretroviral therapy
    Moraima Guadalupe
    Department of Medical Microbiology and Immunology, University of California, Davis, California 95616, USA
    J Virol 77:11708-17. 2003
    ..Monitoring of the restoration and/or maintenance of CD4(+) T cells in GALT provides a more accurate assessment of the efficacy of antiviral host immune responses as well as HAART...
  73. pmc Arginase-1-expressing macrophages suppress Th2 cytokine-driven inflammation and fibrosis
    John T Pesce
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA
    PLoS Pathog 5:e1000371. 2009
  74. ncbi Molecular signature of CD8+ T cell exhaustion during chronic viral infection
    E John Wherry
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road Room G211, Atlanta, GA 30322, USA
    Immunity 27:670-84. 2007
    ..Thus, functional exhaustion is probably due to both active suppression and passive defects in signaling and metabolism. These results provide a framework for designing rational immunotherapies during chronic infections...
  75. pmc Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint
    Q Liu
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 14:1448-59. 2000
    ..Taken together, these data indicate that Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression, and that Atr regulates Chk1...
  76. ncbi hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase
    H Vaziri
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 107:149-59. 2001
    ..In contrast, expression of a catalytically inactive hSir2 protein potentiates p53-dependent apoptosis and radiosensitivity. We propose that hSir2 is involved in the regulation of p53 function via deacetylation...
  77. ncbi Circulating endothelial progenitor cells are reduced in peripheral vascular complications of type 2 diabetes mellitus
    Gian Paolo Fadini
    Department of Clinical and Experimental Medicine, Division of Metabolic Diseases, University of Padova, School of Medicine, Padova, Italy
    J Am Coll Cardiol 45:1449-57. 2005
    ..We sought to establish whether a reduction in endothelial progenitor cells (EPCs) has a putative role in peripheral vascular disease (PVD) of type 2 diabetic patients...
  78. pmc PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 203:2281-92. 2006
  79. ncbi Tumour-derived soluble MIC ligands impair expression of NKG2D and T-cell activation
    Veronika Groh
    Fred Hutchinson Cancer Research Center, Clinical Research Division, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
    Nature 419:734-8. 2002
    ..This mode of T-cell silencing may promote tumour immune evasion and, by inference, compromise host resistance to infections...
  80. ncbi Alternative macrophage activation is essential for survival during schistosomiasis and downmodulates T helper 1 responses and immunopathology
    De Broski R Herbert
    University of Cape Town, Health Sciences Faculty, 7925 Cape Town, South Africa
    Immunity 20:623-35. 2004
    ..Together, this shows that alternative macrophages are essential during schistosomiasis for protection against organ injury through downregulation of egg-induced inflammation...
  81. pmc The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions
    Matthias Nahrendorf
    Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    J Exp Med 204:3037-47. 2007
  82. ncbi Pax7 is required for the specification of myogenic satellite cells
    P Seale
    Department of Biology, McMaster University, Hamilton, Ontario, Canada
    Cell 102:777-86. 2000
    ..Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs...
  83. pmc Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis
    Heather R Conti
    Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo, NY 14201, USA
    J Exp Med 206:299-311. 2009
    ..Thus, the Th17 lineage, acting largely through IL-17, confers the dominant response to oral candidiasis through neutrophils and antimicrobial factors...
  84. pmc CD44+ CD24(-) prostate cells are early cancer progenitor/stem cells that provide a model for patients with poor prognosis
    E M Hurt
    Cancer Stem Cell Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702, USA
    Br J Cancer 98:756-65. 2008
    ..Using flow cytometry, we isolated a population of CD44+CD24(-) prostate cells that display stem cell characteristics as well as ..
  85. ncbi Monocyte emigration from bone marrow during bacterial infection requires signals mediated by chemokine receptor CCR2
    Natalya V Serbina
    Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, Immunology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Nat Immunol 7:311-7. 2006
    ..Thus, CCR2-mediated signals in bone marrow determine the frequency of Ly6C(hi) monocytes in the circulation...
  86. doi RORγt drives production of the cytokine GM-CSF in helper T cells, which is essential for the effector phase of autoimmune neuroinflammation
    Laura Codarri
    Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland
    Nat Immunol 12:560-7. 2011
    ..Thus, in contrast to all other known helper T cell-derived cytokines, GM-CSF serves a nonredundant function in the initiation of autoimmune inflammation regardless of helper T cell polarization...
  87. doi Cross-talk between T cells and NK cells generates rapid effector responses to Plasmodium falciparum-infected erythrocytes
    Amir Horowitz
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    J Immunol 184:6043-52. 2010
  88. ncbi Regulatory T cell lineage specification by the forkhead transcription factor foxp3
    Jason D Fontenot
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Immunity 22:329-41. 2005
    ..Analysis of Foxp3 expression during thymic development suggests that this mechanism is not hard-wired but is dependent on TCR/MHC ligand interactions...
  89. pmc Foxo1 links homing and survival of naive T cells by regulating L-selectin, CCR7 and interleukin 7 receptor
    Yann M Kerdiles
    Molecular Biology Section, Division of Biological Sciences, Department of Cellular and Molecular Medicine, University of California, San Diego, California 92093 0377, USA
    Nat Immunol 10:176-84. 2009
    ..These data suggest that Foxo1 regulates the homeostasis and life span of naive T cells by sensing growth factor availability and regulating homing and survival signals...
  90. ncbi Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
    John R S Newman
    Howard Hughes Medical Institute, San Francisco, California, USA
    Nature 441:840-6. 2006
    ..Here we present a strategy that pairs high-throughput flow cytometry and a library of GFP-tagged yeast strains to monitor rapidly and precisely protein levels at single-cell ..
  91. pmc Vpu enhances HIV-1 virus release in the absence of Bst-2 cell surface down-modulation and intracellular depletion
    Eri Miyagi
    Laboratory of Molecular Microbiology, Viral Biochemistry Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 106:2868-73. 2009
  92. pmc Induction of interleukin 10-producing, nonproliferating CD4(+) T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells
    H Jonuleit
    Department of Dermatology, University of Mainz, D 55101 Mainz, Germany
    J Exp Med 192:1213-22. 2000
    ..These data show that immature and mature DCs induce different types of T cell responses: inflammatory Th1 cells are induced by mature DCs, and IL-10-producing T cell regulatory 1-like cells by immature DCs...
  93. ncbi Analysis of apoptosis by propidium iodide staining and flow cytometry
    Carlo Riccardi
    Department of Clinical and Experimental Medicine, School of Medicine, University of Perugia, and Foundation IBiT, 06122 Perugia, Italy
    Nat Protoc 1:1458-61. 2006
    ..For this reason, since its publication, the PI assay has been widely used, as demonstrated by the large number of citations of the original paper and/or the continuous use of the method in many laboratories...
  94. pmc Identification of an IL-17-producing NK1.1(neg) iNKT cell population involved in airway neutrophilia
    Marie Laure Michel
    Unité Mixte de Recherche 8147, Centre National de la Recherche Scientifique, Faculté de Médecine René Descartes, Paris V, Hopital Necker, Paris, Cedex 15, France
    J Exp Med 204:995-1001. 2007
    ..Collectively, our findings reveal that NK1.1(neg) iNKT lymphocytes represent a new population of IL-17-producing cells that can contribute to neutrophil recruitment through preferential IL-17 secretion...
  95. ncbi CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs
    R Forster
    Molecular Tumorgenetics and Immunogenetics, Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Cell 99:23-33. 1999
    ..Thus, in order to bring together lymphocytes and dendritic cells to form the characteristic microarchitecture of secondary lymphoid organs, CCR7 is required to rapidly initiate an adoptive immune response...
  96. pmc Interleukin-2 and inflammation induce distinct transcriptional programs that promote the differentiation of effector cytolytic T cells
    Matthew E Pipkin
    Harvard Medical School, Boston, MA 02115, USA Immune Disease Institute, Boston, MA 02115, USA
    Immunity 32:79-90. 2010
    ..Thus, inflammation influences both effector and memory CTL differentiation, whereas persistent IL-2 stimulation promotes effector at the expense of memory CTL development...
  97. pmc Foxp3+ follicular regulatory T cells control the germinal center response
    Michelle A Linterman
    Cambridge Institute for Medical Research and the Department of Medicine, University of Cambridge School of Clinical Medicine, Addenbrooke s Hospital, Cambridge, UK
    Nat Med 17:975-82. 2011
    ..Thus, the T(FH) differentiation pathway is co-opted by T(reg) cells to control the germinal center response...
  98. pmc PD-1 expression in acute hepatitis C virus (HCV) infection is associated with HCV-specific CD8 exhaustion
    Simona Urbani
    Laboratorio Immunopatologia Virale, Unit of Infectious Diseases and Hepatology, Azienda Ospedaliero Universitaria di Parma, Via Gramsci 14, 43100 Parma, Italy
    J Virol 80:11398-403. 2006
    ..Blocking PD-1/PDL-1 interaction with an anti-PDL-1 antibody improved the capacity of expansion of virus-specific CD8 cells...
  99. pmc Central memory self/tumor-reactive CD8+ T cells confer superior antitumor immunity compared with effector memory T cells
    Christopher A Klebanoff
    Howard Hughes Medical Institute, National Institutes of Health Research Scholars Program, Bethesda, MD 20814, USA
    Proc Natl Acad Sci U S A 102:9571-6. 2005
    ..Thus, tumor-reactive CD8+ T cell populations with the phenotypic and functional attributes of T(CM) may be superior to T(EM)/effector T cells for adoptive immunotherapies using concomitant tumor-antigen vaccination...
  100. ncbi Mechanism of CD1d-restricted natural killer T cell activation during microbial infection
    Manfred Brigl
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 4:1230-7. 2003
    ..We propose this mechanism of activation as a major pathway responsible for the rapid activation of NKT cells in different microbial infections...
  101. pmc Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum
    Sean C Bendall
    Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA
    Science 332:687-96. 2011
    b>Flow cytometry is an essential tool for dissecting the functional complexity of hematopoiesis...

Research Grants85

    Larry Sklar; Fiscal Year: 2003
    ..Cellular adhesive interactions under shear in vivo and in vitro can be observed by microscopy. Flow cytometry is already an important tool for studying cell activation...
  2. In Vivo Flow Cytometry to Measure Circulating Tumor Load
    David Benaron; Fiscal Year: 2004
    ..b>Flow cytometry -- ex vivo stained cells flowing past a fixed detector-- is an established technique which has allowed for ..
  3. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
    ..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
  4. The role of Fli1 in T cell function and the pathogenesis of lupus
    Tamara K Nowling; Fiscal Year: 2011
    ..Cell proliferation and apoptosis will be analyzed by standard flow cytometry assays...
  5. DNA Damage and Response in the Bladder Microenvironment.
    Bradley P Dixon; Fiscal Year: 2013
    ..These include a number of core facilities directly relevant to this project such as the flow cytometry core, mass spectrometry core, and vivarium at CCHMC as well as numerous faculty members in the Division of ..
  6. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..down, site-directed mutagenesis, DNA cloning, confocal laser microscopy, enzyme and in vitro kinase assays, flow cytometry, and HPLC...
  7. Collagen X and heparan sulfate proteoglycan in the hematopoietic stem cell niche.
    ELIZABETH G SWEENEY; Fiscal Year: 2012
    ..confirm EO generates the hematopoietic niche and that collagen X mice are defective in this process, and 3) flow cytometry and co-culture assays will confirm and begin to characterize the hematopoietic defects in the perlecan ..
  8. Genistein, microRNAs and kidney cancer
    Rajvir Dahiya; Fiscal Year: 2013
    ..Assays include cell proliferation, flow cytometry, migration, clonogenic survival, in vitro invasion and apoptosis assays...
  9. Cellular Immunity to Category A-C Viruses in Humans
    ROBERT WILLIAM FINBERG; Fiscal Year: 2013
    ..projects funding, and centralized services for clinical studies (subject recruitment and characterization), flow cytometry, and production of MHC proteins for use by the research projects and TOP...
  10. Cell signaling as a leukemia biomarker
    ..cytokines and measuring the fine kinetics of modified epitopes on signaling proteins by immunofluorescence flow cytometry. Leukemia is an evolutionary process that works by clonal selection...
  11. Tumor Microenvironment Interactions in Brain Tumors
    Eric C Holland; Fiscal Year: 2010
    ..a comprehensive experimental strategy combining sophisticated mouse models, imaging, organotypic coculture, flow cytometry, microarray profiling, pharmacological approaches and analysis of patient samples...
    Anisa Shaker; Fiscal Year: 2012
    ..participation in national conferences including DDW, FASEB, and AAI, and instruction in bone marrow transfer, flow cytometry, and laser capture microdissection...
  13. Molecular Epidemiology of DNA Repair in Head and Neck Cancer
    Qingyi Wei; Fiscal Year: 2012
    ..Many genes participate in these two apoptotic pathways, and there is an established flow cytometry method to detect the apoptosis phenotype...
  14. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..These aims will be pursued in tissue culture cells using flow cytometry, confocal microscopy, immunofluorescence, molecular biology, and biochemistry...
  15. Dual-Receptor Targeted Nanoparticles for Photodynamic Therapy of Brain Cancer
    Ann Marie Broome; Fiscal Year: 2013
    ..assays, and cell permeability assays, including trypan blue staining and Annexin V/ propidium iodide flow cytometry. Specific Aim 3: In vivo translation of PDT therapy and post-therapy monitoring in glioma tumor bearing mice...
  16. Hematopoietic Stem Cells (HSCs) as Juvenile Protective Factors that Alter Aging
    David E Harrison; Fiscal Year: 2011
    ..Long term (LT)-HSCs, short term (ST)-HSCs and common myeloid precursors (CMPs) will be identified by flow cytometry markers optimized for aging studies in these strains...
  17. The Role of Interferon-gamma in the Regulation of Hematopoietic Stem Cells
    KATHERINE YUDEH KING; Fiscal Year: 2013
    ..expertise in biostatistics, research conduct and ethics, and state-of-the-art technology in microscopy and flow cytometry. I will participate in local, national, and international meetings in the fields of infectious diseases, ..
  18. The voltage-gated Kv1.3 channel in psoriatic disease: A novel therapeutic target
    Siba P Raychaudhuri; Fiscal Year: 2012
    ..T cells in the pathogenesis of psoriasis and psoriatic arthritis using a combination of immunohistochemistry, flow cytometry and electrophysiology studies. To further substantiate the pathologic role of Kv1...
  19. MicroRNAs in the Progression and Metastisis of Prostate Cancer
    Rajvir Dahiya; Fiscal Year: 2013
    ..Assays to be used include cell proliferation, flow cytometry, migration, clonogenic survival, in vitro invasion and TUNEL-based ELISA apoptosis assays...
  20. Mucosal Immunity and Influenza Vaccines: Phenotype and Role of Activated B Cells
    HARRY BERNARD GREENBERG; Fiscal Year: 2010
    ..LAIV or TIV vaccination and analyze plasmablasts for expression of trafficking receptors using multi-color flow cytometry. Aim 2. Compare the antibodies produced by plasmablasts after LAIV or TIV immunization...
    DONALD JOSEPH MCCRANN; Fiscal Year: 2010
    ..steady-state erythropoiesis, EPO-responsiveness, and anemia in Tsad- null mice using hematological analyses, flow cytometry, and ex vivo pro-erythroblast culture...
  22. Preclinical testing of a novel PDK1 inhibitor for treating vestibular schwannoma
    Abraham Jacob; Fiscal Year: 2013
    ..Using a variety of in vitro laboratory techniques (cytotoxicity assays, flow cytometry, TUNEL, antibody arrays, caspase activity assays, proliferation indices, FACS, RNA interference, PCR and ..
  23. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    ..b>Flow cytometry analysis showed that inhibition of decatenation activates ATM in PSCs and arrests them in G2...
  24. DNA Methylation Control Hematopoietic Stem Cell Lineage Differentiation
    GRANT ANTHONY CHALLEN; Fiscal Year: 2013
    ..Medicine (STaR) Center, I will have access to diverse core facilities with expertise in microarray, flow cytometry, microscopy and embryonic stem cells as well as access to a high-level, pathogen-free barrier utilizing ..
  25. Regulation of Fibronectin Matrix Assembly by uPAR
    PAULA J MC KEOWN-LONGO; Fiscal Year: 2012
    ..sequestration and degradation in response to uPAR or EGFR ligands will be analyzed by Western blot, FLOW cytometry and confocal microscopy...
  26. K-Channels in Lymphocyte Function and Autoimmunity
    ..In Aim 3 we will use patch-clamp recording and flow cytometry to determine whether Kv1...
  27. The role of SIRT1/FOXO4 pathway in podocyte apoptosis of diabetes mellitus
    PETER YENLUNG CHUANG; Fiscal Year: 2013
    ..Apoptosis will be assessed by flow cytometry, TUNEL staining, and caspase assays. Gene expression will be quantified by real-time PCR and Western blot...
  28. RYGB Improves Metabolism by Interrupting the Gastric Adipose Tissue Axis
    Naji N Abumrad; Fiscal Year: 2013
    ..pro- and anti-oxidative stress enzymes in adipose tissue biopsies (b) adipose tissue macrophage content via flow cytometry, RT-qPCR and immunohistochemistry (c) cellular factors involved in insulin signaling in adipose tissue and ..
  29. Biologic Activities of IL-36 Cytokines in Psoriasis
    Andrew Johnston; Fiscal Year: 2013
    ..ft. of lab space furnished with equipment for cell culture, RNA isolation, PCR, flow cytometry. I have the mentorship of Dr...
  30. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..unstimulated endothelial cells, and localized to either the cell surface or internal organelles, or both, by flow cytometry, immunofluorescence microscopy and electron microscopy...
  31. WI State Public Health Lab Capacity for SCID Screening
    CHARLES DENNIS BROKOPP; Fiscal Year: 2010
    ..TRECs assay and 2-actin measurement, (both on the initial DBS), a second whole blood specimen is assessed by flow cytometry (CD3, CD4, CD8, CD19, CD56, CD45 and CD45RO);low counts warrant immediate referral and assessment at the ..
  32. Epigenetic regulation of stem cell senescence in aging
    DAVID TOCHUKWU ASUZU; Fiscal Year: 2011
    ..will quantify ICC and ICC precursors in gastric muscles from the klotho mouse, a model of premature aging, by flow cytometry. In two established ICC stem cell lines, I will study gene expression and the role of Wnt signaling and its ..
  33. Endothelial Progenitor Cells in Brain Arteriovenous Malformations
    Yongmei Chen; Fiscal Year: 2010
    ..The level of circulating EPCs will be quantified by flow cytometry in blood obtained preoperatively. EPC function will be determined by colony forming units assay...
  34. Rapid Analysis of Single T Cell Immunity Signatures in Tuberculosis
    Sanjay Tyagi; Fiscal Year: 2013
    ..with (ii) measurement of inducible tell-tale markers of T cell activation and function by quantitative flow cytometry. Induced gene expression will be detected by mRNA enumeration using single molecule fluorescence in situ ..
  35. NKT cells, fatty acids, and insulin resistance
    CURTIS LEE GABRIEL; Fiscal Year: 2010
    ..this goal, we will feed mice a lard diet, a fish oil diet or a low-fat diet, and will analyze NKT cells using flow cytometry, ELISA and ex vivo stimulation assays...
    Kafait U Malik; Fiscal Year: 2013
    ..function;4) histological, immunohistochemical, and fluorescence microscopy and biochemical techniques;5) flow cytometry to determine immune cell population in the blood and tissues;6) T cells from the spleen of transgenic mice to ..
  37. High-throughput multiplex microsphere screening for toxin protease inhibitors
    Steven W Graves; Fiscal Year: 2012
    ..To discover active compounds for these proteases we developed a high throughput flow cytometry based protease assay to perform pilot screens of the Prestwick Library, which identified 3 lead inhibitors for ..
  38. Differentiation of Cord Blood Mesenchymal Stem Cells to Corneal Endothelium
    Nancy C Joyce; Fiscal Year: 2010
    ..Microarray analysis will determine relative gene expression, flow cytometry will identify surface marker expression, Western blots will compare expression of specific cellular and matrix ..
    ..Our flow cytometry results further demonstrate that each HK mutant is unable to properly coordinate DNA replication initiation ..
  40. Regulation of TSLP receptor expression and function in eczema in mice and man
    Steven F Ziegler; Fiscal Year: 2013
    ..will be tested for their capacity to upregulate TSLPR and the relevant signaling components elucidated using flow cytometry and gene silencing techniques...
  41. Regenerating Human Oral Mucosa by Bone Marrow-derived Mesenchymal Stem Cells
    RUBIE ANN RAKIAN; Fiscal Year: 2013
    ..provide training in stem cell maintenance and differentiation, quantitative gene expression, immunochemistry, flow cytometry, FACS sorting, animal modeling, and cell culture...
  42. The Role of Syntrophic Bacteria in Methanogenic Metabolism in the Human Gut
    Catherine Lozupone; Fiscal Year: 2013
    ..In Situ Hybridization (FISH) and 2) metagenomic sequencing of cell populations that were concentrated using flow cytometry. Specific Aim 3 further explores the underlying cause of co-occurrence patterns by developing and applying ..
  43. Th2 Cytokines and Signaling in Pediatric Inflammatory Bowel Disease
    Michael J Rosen; Fiscal Year: 2013
    ..This project will advance our understanding of the influence of Th2 inflammation on therapeutic outcome in children with IBD and determine the potential of the Th2-inducing cytokine IL-33 as a target for future UC therapy. ..
  44. Immune Regulation of CNS Viral Recrudescence
    Cornelia Bergmann; Fiscal Year: 2010
    ..Using a novel transgenic mouse, termed Blimpgfp/+, a combination of flow cytometry and ELISPOT techniques will characterize GFP+ ASC populations unique to the CNS and their potential to ..
  45. Biophysics of Drug Interactions with Viral Episomes
    James K Bashkin; Fiscal Year: 2013
    ..and provide absolute binding constants with the aid of PA concentrations in cells measured by confocal and flow cytometry methods. Measure viral DNA concentrations vs...
  46. Age-dependent microglial responses in hypoxia-ischemia
    PETER A FERRAZZANO; Fiscal Year: 2013
    ..and neuroimaging, and provides core facility resources for animal imaging, behavioral testing, microscopy, and flow cytometry. We have assembled a team of experienced mentors with expertise in the fields vital to my research: molecular ..
  47. Angiogenic tissue engineering to limit post-infarction ventricular remodeling
    Y Joseph Woo; Fiscal Year: 2012
    ..EPCs will be tracked with flow cytometry and immunohistochemistry. Angiogenesis will be quantified with lectin microangiography...
  48. GABA chaperoning of epilepsy-associated GABAA receptor mutants.
    Nancy J Leidenheimer; Fiscal Year: 2012
    ..receptor cell surface expression will be measured using immunofluorescence confocal microscopy and flow cytometry. PUBLIC HEALTH RELEVANCE: The GABAA receptor is a neurotransmitter receptor that can carry various genetic ..
    Stephen W Chensue; Fiscal Year: 2013
    ..cell transfer, preparation of bone marrow chimeras, mDC preparation and culture, gene expression analysis, and flow cytometry. Mycobacterial and helminth infections are responsible for a profound degree of global morbidity and mortality...
  50. Toll-like Receptor 2, Vitamin D, and Extrapulmonary Tuberculosis
    CHRISTINA A FISKE; Fiscal Year: 2013
    ..Measurements of quantity and function will be performed using polymerase chain reaction (PCR) and flow cytometry. Results will be compared to results obtained from persons with previous pulmonary TB, persons with latent M...
  51. The Role of CRACM1 in the Pathogenesis of Systemic Lupus Erythematosus
    Peter C Chien; Fiscal Year: 2013
    ..to controls, T cells will be stained with fluorescently labeled anti-CRACM1 antibodies and then analyzed by flow cytometry. Immunophenotyping will also determine which subpopulations of T cells may be expressing CRACM1...
  52. FAMU RCMI Pharmaceutical Research Center
    Karam F A Soliman; Fiscal Year: 2013
    ..services in proteomics;(peptide sequencing, protein identification, post translational modifications), flow cytometry, imaging, high throughput screening and other advanced biomedical applications;(2) Drug Discovery Core ..
  53. Cerebral Artery Alpha1 Adrenergic and PKC Regulatory Mechanisms
    Lawrence D Longo; Fiscal Year: 2013
    ..contractility and intracellular [Ca2+] measurements, Western immunoblots, RT- PCR, confocal microscopy, flow cytometry, 2D-gel-mass spectroscopy, gene silencing by double stranded RNA or morpholinos, gene upregulation, gene ..
  54. Polymorphisms of estrogen-metabolizing genes in kidney cancer
    Yuichiro Tanaka; Fiscal Year: 2013
    ..cell proliferation, apoptosis, cell invasion, and cell cycle distribution in EMG-treated renal cell lines by flow cytometry and biological assays, (d) Evaluate the effects of EMG on cell morphology and expression of various genes by ..
  55. System for Rapid Generation of Aptamers for Transplant Therapies
    KAREN QIAN; Fiscal Year: 2010
    ..We will measure the binding efficiency and specificity to CD34+ cells with flow cytometry, and perform magnetic cell selection of spiked CD34+ cells in whole blood...
  56. Proteolytic Pathways in Thrombus Resolution
    Toni M Antalis; Fiscal Year: 2013
    ..Venous thrombi will be analyzed by immunohistochemistry, flow cytometry, mRNA and protein analyses for molecular indicators of inflammation and thrombus resolution...
  57. Role of Interleukin-1 in mediating epithelial-dendritic cell crosstalk in the lun
    BART N contact LAMBRECHT; Fiscal Year: 2010
    ..These analyses involve the use of cutting edge flow cytometry and imaging of the dynamic behavior of DCs in vital tracheal tissue using dual- photon microscopy...
  58. Pulmonary Vascular Changes in Early Chronic Obstructive Pulmonary Disease (COPD)
    R Graham Barr; Fiscal Year: 2011
    ..severe COPD, and 100 healthy controls) will be characterized with MR and full-lung CT imaging, spirometry, flow cytometry, and gene expression profiling to test the following hypotheses: 1) Functional and structural changes in the ..
  59. Chronic Toll-like Receptor Ligation and Hematopoietic Stem Cell Aging
    Brandt L Esplin; Fiscal Year: 2010
    ..Multi-color flow cytometry and molecular assays such as western blot and RT-PCR will reveal whether chronic TLR stimulation induces ..
  60. Heterologous abs from llama and chicken egg yolk to prevent rotavirus diarrhea
    Linda J Saif; Fiscal Year: 2010
    ..Students from INTA will be trained in Dr. Saif's lab to learn newer immunologic procedures (flow cytometry, etc) and work with specialized animal disease models (Gn pigs) as well as to enhance analytical skills ..
  61. Structure and function analysis of C1/C1q and MBL using a novel peptide inhibitor
    NEEL KUMAR KRISHNA; Fiscal Year: 2012
    ..function by: (a) determining the ability of CPP to inhibit C1q binding to CRT/cC1qR on neutrophils utilizing flow cytometry and (b) characterizing the biological consequence of CPP disruption of the C1q-CRT/cC1qR interaction on ..
  62. Stem Cell Based Therapy for Regenerative Endodontics
    George T J Huang; Fiscal Year: 2013
    ..Procedures include flow cytometry, real-time RT-PCR, Western blot, immunohistochemical analysis, etc...
  63. Naltrexone and AIDS progression
    GREGORY MICHAEL MILLER; Fiscal Year: 2013
    ..exhaustion markers expressed by circulating memory CD4+ and CD8+ T cells will be determined by a polychromatic flow cytometry panel that includes antibodies to CD3, CD4, CD28, CD95, CCR7, CCR5, CD69, HLA-DR and PD-1, and monitored every ..
  64. Role of complement in alpha-synuclein based models of Parkinson disease
    HEATHER ELIZABETH ALLEN; Fiscal Year: 2013
    ..protein expression of complement components expressed by microglia, changes in surface complement receptors by flow cytometry, changes in phagocytosis by commercially available assays, and changes in cytokine expression by ELISA...
  65. Identification and Characterization of Adult Salivary Gland Stem Cells
    Soosan Ghazizadeh; Fiscal Year: 2013
    ..We will then recover these slowly-cycling cells by flow cytometry and assess their regenerative abilities;2) we will utilize lentiviral-mediated in situ genetic marking and ..
    Hasan Mukhtar; Fiscal Year: 1999
    ..fragmentation, histology, direct immunoperoxidase staining of digoxigenin-labeled genomic DNA in sections, or flow cytometry. 2) The cellular target of apoptosis will be studied in RIF-1 tumors in which apoptosis occurs during PcPDT ..
    Larry Sklar; Fiscal Year: 2002
    ..Cellular adhesive interactions under shear in vivo and in vitro can be observed by microscopy. Flow cytometry is already an important tool for studying cell activation...
    GEORGE DEGEORGE; Fiscal Year: 2000
    ..The purpose of this proposal is to determine the feasibility of applying flow cytometry to enhance the LLNA...
    ROSS BASCH; Fiscal Year: 1991
    ..is for fund to a FACStarplus flow cytometer/cell sorter to replace the Ortho 50H now operated by the NYU Flow Cytometry Facility. The Ortho 50H, while remarkably advanced for its time, is an awkward instrument to use as a sorter...
  70. A High-throughput Screen of Lytic Granule Exocytosis
    Adam Zweifach; Fiscal Year: 2012
    ..protein 1 (LAMP-1) from lytic granules to the plasma membrane in a simple no-wash assay protocol suitable for flow cytometry. We plan to capitalize on this simple assay together with the unique HTS flow cytometry capabilities at the ..
  71. Microfluidic Processing of Leukocytes for Molecular Diagnostic Testing
    James C Sturm; Fiscal Year: 2012
    ..pathway status of multiple types of normal leukocytes vs leukemia cells simultaneously, by multi- parameter flow cytometry or in the future, atomic mass spectrometry...
    Patricia Jones; Fiscal Year: 1999
    ..Sciences seeks a NIH-NCRR Shared Instrumentation grant to purchase a flow cytometer/cell sorter for its Flow Cytometry Facility...
    Dennis Van Epps; Fiscal Year: 1990
    This proposal will use fluorescent chemotactic factors and techniques of flow cytometry and quantitative video intensification microscopy (QUAVIM) to study human neutrophil (PMN) and monocyte function and dysfunction...
    John Schroeder; Fiscal Year: 2002
    ..b>Flow cytometry will be used to determine intracellular cytokine expression...
    Dennis Van Epps; Fiscal Year: 1990
    ..Responses will be evaluted using specific fluorescent probes and quantitative flow cytometry and will allow us to determine which lymphocyte subsets respond to LCF...
  76. Targeting the B Cell Receptor Signaling Network in Lymphoma
    Jonathan Irish; Fiscal Year: 2009
    ..of technologies, including measurement of signaling in individual cells from primary human tissues by flow cytometry and computational modeling of the BCR signaling network, will be used to achieve this goal...
  77. High-speed Flow Cytometer
    Kenneth Weinberg; Fiscal Year: 2003
    ..Because flow cytometry has been a key methodologic tool for the research performed by the GIST investigators, the program has built ..
  78. BD Facsaria for use in BSL3
    Padmini Salgame; Fiscal Year: 2009
    ..Centre for Emerging and Re-emerging Pathogens at NJMS and will be operated and maintained by an experienced flow cytometry technician, Ms. Angelika Batista. The Principal Investigator, Dr...
  79. Cell Sorting and Molecular Analysis for Minimal Disease Detection
    Barbara Zehentner; Fiscal Year: 2006
    ..by applicant): Project Summary: The aim of this study is to validate the novel technical application of flow cytometry and cell sorting combined with molecular analysis to detect and to confirm low levels of disease in ..
  80. Amnis ImageStream Cell Analysis System
    Patricia Fitzgerald Bocarsly; Fiscal Year: 2007
    ..Instrument Grant proposal is for the purchase of an Amnis ImageStream instrument that combines the power of flow cytometry with the capture of high resolution digital images of cells in flow...
  81. FACS Aria II Three Laser Special Order System
    DESCRIPTION (provided by applicant): The University of Nebraska-Lincoln (UNL) flow cytometry core facility requests funds to replace its FACS Vantage SE flow cytometer/cell sorter (which is obsolete) with a FACS Aria II three laser ..
  82. Validation of a Vascular Health Profile for Cardiovascular Disease
    Emile R Mohler; Fiscal Year: 2010
    ..recently completed a pilot study evaluating levels of MPs in patients with diabetes mellitus (DM) and compared flow cytometry results with those of a non cell specific Enzyme Linked ImmunoSorbent assay (ELISA)...
  83. Beckman Coulter BioMek 2000 Workstation and Accessories
    Joseph McCune; Fiscal Year: 2002
    For the automation of flow cytometry-based assays of immune function, funding is requested to purchase a Beckman Coulter BioMek 2000 system, to be located in the Gladstone/UCSF Core Immunology Laboratory at San Francisco General Hospital...
  84. Biological Definition of FCRL Molecules in Malignancy
    Randall Davis; Fiscal Year: 2009
    ..of patients into indolent or more aggressive cohorts;however, analysis of this cytoplasmic marker by flow cytometry has created technical challenges resulting in diagnostic variability...
    ROBYN MCGUIRE; Fiscal Year: 1999
    ..A flow cytometry assay that takes days instead of weeks was developed for the phenotypic determination of drug susceptibility ..