cell separation


Top Publications

  1. ncbi Adipose-derived stem cells for regenerative medicine
    Jeffrey M Gimble
    Stem Cell Biology Laboratory and Clinical Nutrition Research Unit, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    Circ Res 100:1249-60. 2007
  2. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
  3. ncbi Circulating tumor cells, disease progression, and survival in metastatic breast cancer
    Massimo Cristofanilli
    Department of Breast Medical Oncology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    N Engl J Med 351:781-91. 2004
  4. pmc Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma
    M E Prince
    Department of Otolaryngology Head and Neck Surgery, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 104:973-8. 2007
  5. ncbi Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion
    Akira Orimo
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 121:335-48. 2005
  6. ncbi Identification of human brain tumour initiating cells
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, M5G 1X8, Canada
    Nature 432:396-401. 2004
  7. pmc Isolation of rare circulating tumour cells in cancer patients by microchip technology
    Sunitha Nagrath
    Surgical Services and BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts 02114, USA
    Nature 450:1235-9. 2007
  8. ncbi Mesenchymal stem cells reside in virtually all post-natal organs and tissues
    Lindolfo da Silva Meirelles
    Departamento de Genetica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    J Cell Sci 119:2204-13. 2006
  9. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
  10. ncbi Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004

Detail Information

Publications361 found, 100 shown here

  1. ncbi Adipose-derived stem cells for regenerative medicine
    Jeffrey M Gimble
    Stem Cell Biology Laboratory and Clinical Nutrition Research Unit, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    Circ Res 100:1249-60. 2007
    ..The isolation, characterization, and preclinical and clinical application of adipose-derived stem cells (ASCs) are reviewed in this article...
  2. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
    ..These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential...
  3. ncbi Circulating tumor cells, disease progression, and survival in metastatic breast cancer
    Massimo Cristofanilli
    Department of Breast Medical Oncology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    N Engl J Med 351:781-91. 2004
    ..We tested the hypothesis that the level of circulating tumor cells can predict survival in metastatic breast cancer...
  4. pmc Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma
    M E Prince
    Department of Otolaryngology Head and Neck Surgery, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 104:973-8. 2007
  5. ncbi Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion
    Akira Orimo
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 121:335-48. 2005
    ..Our findings indicate that fibroblasts within invasive breast carcinomas contribute to tumor promotion in large part through the secretion of SDF-1...
  6. ncbi Identification of human brain tumour initiating cells
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, M5G 1X8, Canada
    Nature 432:396-401. 2004
  7. pmc Isolation of rare circulating tumour cells in cancer patients by microchip technology
    Sunitha Nagrath
    Surgical Services and BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts 02114, USA
    Nature 450:1235-9. 2007
    ..It has broad implications in advancing both cancer biology research and clinical cancer management, including the detection, diagnosis and monitoring of cancer...
  8. ncbi Mesenchymal stem cells reside in virtually all post-natal organs and tissues
    Lindolfo da Silva Meirelles
    Departamento de Genetica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    J Cell Sci 119:2204-13. 2006
    ..These findings have implications for understanding MSC biology, and for clinical and pharmacological purposes...
  9. pmc ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome
    Christophe Ginestier
    Department of Internal Medicine, Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Stem Cell 1:555-67. 2007
    ..These findings offer an important new tool for the study of normal and malignant breast stem cells and facilitate the clinical application of stem cell concepts...
  10. ncbi Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
    ..In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow...
  11. pmc Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
    Shannon L Stott
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 107:18392-7. 2010
    ..In a subset of patient samples, the low shear design of the HB-Chip revealed microclusters of CTCs, previously unappreciated tumor cell aggregates that may contribute to the hematogenous dissemination of cancer...
  12. ncbi Purification and characterization of mouse hematopoietic stem cells
    G J Spangrude
    Department of Pathology, Stanford University School of Medicine, CA 94305
    Science 241:58-62. 1988
    ..Thirty of these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the survivors...
  13. pmc Isolation by size of epithelial tumor cells : a new method for the immunomorphological and molecular characterization of circulatingtumor cells
    G Vona
    INSERM U 370, Faculte Necker, Paris, France
    Am J Pathol 156:57-63. 2000
    ..Overall, ISET constitutes a novel approach that should open new perpectives in molecular medicine...
  14. ncbi Isolation of amniotic stem cell lines with potential for therapy
    Paolo De Coppi
    Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1094, USA
    Nat Biotechnol 25:100-6. 2007
  15. pmc EpCAM-positive hepatocellular carcinoma cells are tumor-initiating cells with stem/progenitor cell features
    Taro Yamashita
    Liver Carcinogenesis Section, Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA
    Gastroenterology 136:1012-24. 2009
    ..We hypothesized that the heterogeneous nature of hepatocellular carcinoma (HCC), in part, may be owing to the presence of hepatic cancer cells with stem/progenitor features...
  16. pmc Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    J Immunol Methods 329:112-24. 2008
  17. ncbi Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
    Yanming Bi
    Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, US National Institutes of Health, 30 Convent Dr 30 225 MSC 4320, Bethesda, Maryland 20892, USA
    Nat Med 13:1219-27. 2007
    ..Our results, while offering new insights into the biology of tendon cells, may assist in future strategies to treat tendon diseases...
  18. pmc Epigenetic control of the foxp3 locus in regulatory T cells
    Stefan Floess
    Experimentelle Rheumatologie, Charite Universitaetsmedizin Berlin, Berlin, Germany
    PLoS Biol 5:e38. 2007
  19. ncbi Identification and characterization of tumorigenic liver cancer stem/progenitor cells
    Stephanie Ma
    Department of Pathology, The University of Hong Kong, Pokfulam, Hong Kong, China
    Gastroenterology 132:2542-56. 2007
    ..The aim of this study is to identify, isolate, and characterize the CSC population that drives and maintains hepatocellular carcinoma (HCC) growth and metastasis...
  20. pmc Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Exp Med 206:2483-96. 2009
    ..Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities...
  21. ncbi Isolation and expansion of adult cardiac stem cells from human and murine heart
    Elisa Messina
    Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy
    Circ Res 95:911-21. 2004
  22. pmc 3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood
    Siyang Zheng
    Department of Bioengineering, Pennsylvania State University, University Park, PA 16802, USA
    Biomed Microdevices 13:203-13. 2011
    ..The device provides a highly valuable tool for assessing and characterizing viable enriched circulating tumor cells in both research and clinical settings...
  23. ncbi A population of very small embryonic-like (VSEL) CXCR4(+)SSEA-1(+)Oct-4+ stem cells identified in adult bone marrow
    M Kucia
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 20:857-69. 2006
    ..We hypothesize that this population of Sca-1(+)lin(-)CD45- very small embryonic-like (VSEL) stem cells is deposited early during development in BM and could be a source of pluripotent stem cells for tissue/organ regeneration...
  24. ncbi Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow
    Stan Gronthos
    Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia
    J Cell Sci 116:1827-35. 2003
    ..Using a combination of cell separation techniques, we have isolated an almost homogeneous population of BMSSCs from adult human bone marrow...
  25. ncbi Endothelial progenitor cells: identity defined?
    Frank Timmermans
    Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, University Hospital Ghent, De Pintelaan, Ghent, Belgium
    J Cell Mol Med 13:87-102. 2009
    ..We also discuss our recent findings on the origin of endothelial outgrowth cells (EOCs), showing that this in vitro defined EC population does not originate from circulating CD133(+) cells or CD45(+) haematopoietic cells...
  26. ncbi Purification and unique properties of mammary epithelial stem cells
    John Stingl
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver V5Z 1L3, British Columbia, Canada
    Nature 439:993-7. 2006
    ..The mammary stem cells are also a rapidly cycling population in the normal adult and have molecular features indicative of a basal position in the mammary epithelium...
  27. ncbi Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87
    Shi cang Yu
    Institute of Pathology, Southwest Hospital, Third Military Medical University, Gaotanyan 30, Shapingba District, Chongqing 400038, PR China
    Cancer Lett 265:124-34. 2008
  28. pmc Label-free cell separation and sorting in microfluidic systems
    Daniel R Gossett
    Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, CA 90095 1600, USA
    Anal Bioanal Chem 397:3249-67. 2010
    b>Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods...
  29. pmc Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line
    Toru Kondo
    Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Proc Natl Acad Sci U S A 101:781-6. 2004
    ..We propose that many cancer cell lines contain a minor subpopulation of stem cells that is enriched in an SP, can be maintained indefinitely in culture, and is crucial for their malignancy...
  30. ncbi Double spiral microchannel for label-free tumor cell separation and enrichment
    Jiashu Sun
    CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China
    Lab Chip 12:3952-60. 2012
    This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces...
  31. ncbi Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation
    Ali Asgar S Bhagat
    BioSystems and Micromechanics IRG, Singapore MIT Alliance for Research and Technology Centre, Singapore
    Lab Chip 11:1870-8. 2011
  32. ncbi Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages
    S I Nishikawa
    Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan
    Development 125:1747-57. 1998
    ..This line of evidence implicates Flk1+VE-cadherin+ cells as a diverging point of hemopoietic and endothelial cell lineages...
  33. ncbi Laser-capture microdissection
    Virginia Espina
    Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd MS 4E3, Manassas, Virginia, USA
    Nat Protoc 1:586-603. 2006
    ..Herein we provide a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1-1.5 h...
  34. ncbi Dystrophin expression in the mdx mouse restored by stem cell transplantation
    E Gussoni
    Division of Genetics, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 401:390-4. 1999
    ..Our studies also suggest that the inherent developmental potential of stem cells isolated from diverse tissues or organs may be more similar than previously anticipated...
  35. ncbi High-throughput selection, enumeration, electrokinetic manipulation, and molecular profiling of low-abundance circulating tumor cells using a microfluidic system
    Udara Dharmasiri
    Center for Bio Modular Multi Scale Systems, Louisiana State University, 8000 GSRI Road, Building 3100, Baton Rouge, Louisiana 70820 7403, United States
    Anal Chem 83:2301-9. 2011
    ..However, the HT29 cells did not contain these mutations, consistent with their known genotype...
  36. ncbi Very small embryonic-like stem cells with maximum regenerative potential get discarded during cord blood banking and bone marrow processing for autologous stem cell therapy
    Deepa Bhartiya
    Stem Cell Biology Department, National Institute for Research in Reproductive Health, Parel, Mumbai, India
    Stem Cells Dev 21:1-6. 2012
  37. ncbi Stromal cell-derived factor-1 effects on ex vivo expanded endothelial progenitor cell recruitment for ischemic neovascularization
    Jun Ichi Yamaguchi
    Division of Cardiovascular Research and Medicine, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 107:1322-8. 2003
    ..Given the close relationship between hematopoietic stem cells and endothelial progenitor cells (EPCs), we investigated the effect of SDF-1 on EPC-mediated vasculogenesis...
  38. ncbi Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells
    Elena A Jones
    University of Leeds, Leeds, UK
    Arthritis Rheum 46:3349-60. 2002
    ..The aim of this study was to isolate and characterize human bone marrow (BM) MPCs...
  39. ncbi A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability
    Peter Eirew
    Terry Fox Laboratory, British Columbia Cancer Agency, 675 West Tenth Avenue, Vancouver, British Columbia, Canada
    Nat Med 14:1384-9. 2008
    ..This new assay procedure sets the stage for investigations of mechanisms regulating normal human mammary stem cells (and possibly stem cells in other tissues) and their relationship to human cancer stem cell populations...
  40. pmc Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration
    Zhuqing Qu-Petersen
    Growth and Development Laboratory, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh, PA 15260, USA
    J Cell Biol 157:851-64. 2002
    ..Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell-mediated therapies...
  41. ncbi Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research?
    Andrea Romana Prusa
    Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Hum Reprod 18:1489-93. 2003
    ..Oct-4 is a marker for pluripotent human stem cells so far known to be expressed in embryonal carcinoma cells, embryonic stem cells and embryonic germ cells...
  42. ncbi Isolation and characterization of a stem cell population from adult human liver
    Maria Beatriz Herrera
    Department of Internal Medicine, University of Torino, Torino, Italy
    Stem Cells 24:2840-50. 2006
    ..In conclusion, we here identified a pluripotent progenitor population in adult human liver that could provide a basis for cell therapy strategies...
  43. pmc Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem cell antigen-1
    Venkata Lokesh Battula
    University of Tubingen, Department of Internal Medicine II, Medical, Otfried Muller Str 10, 72076, Tubingen, Germany
    Haematologica 94:173-84. 2009
  44. ncbi Novel markers for the prospective isolation of human MSC
    Hans Jorg Buhring
    Department of Internal Medicine II, University Clinic, Medical Research Center, Otfried Müller Str 27, 72076 Tubingen, Germany
    Ann N Y Acad Sci 1106:262-71. 2007
    ..These MSC may be used as an improved starting population for transplantation in diseases like osteogenesis imperfecta, cartilage repair, and myocardial infarction...
  45. pmc Direct isolation of human central nervous system stem cells
    N Uchida
    StemCells, Inc, 525 Del Rey Avenue, Suite C, Sunnyvale, CA 94085 Laboratory of Genetics, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 97:14720-5. 2000
    ..Upon transplantation into brains of immunodeficient neonatal mice, the sorted/expanded hCNS-SC showed potent engraftment, proliferation, migration, and neural differentiation...
  46. ncbi Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection
    Melody Baddoo
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Cell Biochem 89:1235-49. 2003
    ..Therefore, FGF2 appears to function as a mitogen and self-maintenance factor for murine MSCs enriched from bone marrow by negative selection...
  47. ncbi Bone marrow stromal cells generate muscle cells and repair muscle degeneration
    Mari Dezawa
    Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoecho, Sakyo ku, Kyoto, 606 8501 Japan
    Science 309:314-7. 2005
    ..These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow...
  48. pmc Molecular signatures of proliferation and quiescence in hematopoietic stem cells
    Teresa A Venezia
    Cell and Molecular Biology Program, Baylor College of Medicine, Houston, Texas, USA
    PLoS Biol 2:e301. 2004
    ..These data will be of use in attempts to recapitulate the HSC self renewal process for therapeutic expansion of stem cells, and our model may correlate with acquisition of self renewal characteristics by cancer stem cells...
  49. ncbi Deformability based cell margination--a simple microfluidic design for malaria-infected erythrocyte separation
    Han Wei Hou
    Division of Bioengineering, National University of Singapore, 7 Engineering Drive 1, Singapore, 117574, Singapore
    Lab Chip 10:2605-13. 2010
    ..g. sickle cell anemia and leukemia, characterized by changes in cell stiffness...
  50. ncbi Mobilization of bone marrow-derived Oct-4+ SSEA-4+ very small embryonic-like stem cells in patients with acute myocardial infarction
    Wojciech Wojakowski
    Third Division of Cardiology, Medical University of Silesia, Katowice, Poland
    J Am Coll Cardiol 53:1-9. 2009
    ..This study sought to assess of the mobilization of nonhematopoietic very small embryonic-like stem cells (VSELs) in acute myocardial infarction (MI)...
  51. pmc The RNAseIII enzyme Drosha is critical in T cells for preventing lethal inflammatory disease
    Mark M W Chong
    The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA
    J Exp Med 205:2005-17. 2008
    ..Thus, miRNA-dependent regulation is critical for preventing spontaneous inflammation and autoimmunity...
  52. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
    ..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
  53. pmc Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase
    Matthew R Clay
    Department of Molecular and Cell Biology, University of Wisconsin, Madison, Wisconsin, USA
    Head Neck 32:1195-201. 2010
    ..We previously reported that CD44 isolates tumorigenic cells from head and neck squamous cell cancer (HNSCC). Recent studies indicate that aldehyde dehydrogenase (ALDH) activity may represent a more specific marker of CSCs...
  54. ncbi Origin of stem cells in organogenesis
    J M W Slack
    Stem Cell Institute, University of Minnesota, McGuire Translational Research Facility, 2001 6th Street SE, Minneapolis, MN 55455, USA
    Science 322:1498-501. 2008
    ..Possible explanations for the existence of such cells, and the issue of whether they also exist in vivo, are discussed...
  55. pmc Detection and characterization of CD133+ cancer stem cells in human solid tumours
    Virginia Tirino
    Dipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia, Seconda Universita degli Studi di Napoli, Napoli, Italy
    PLoS ONE 3:e3469. 2008
    ..Therefore, our aim was to observe the possible presence of cells expressing the CD133 antigen within solid tumour cell lines of osteosarcoma and, then, understand their biological characteristics and performances...
  56. ncbi Microdevice for the isolation and enumeration of cancer cells from blood
    Swee Jin Tan
    Institute of Microelectronics, A STAR Agency for Science, Technology and Research, 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore
    Biomed Microdevices 11:883-92. 2009
    ..The microdevice has the potential to be used for routine monitoring of cancer development and cancer therapy in a clinical setting...
  57. ncbi Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol
    Ming Song Tsai
    Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan
    Hum Reprod 19:1450-6. 2004
    ..The aim of this study was to isolate mesenchymal stem cells (MSCs) from amniotic fluid obtained by second-trimester amniocentesis...
  58. ncbi Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential
    Alexandra Peister
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Blood 103:1662-8. 2004
    ..The differences among MSCs from different strains may explain some of the conflicting data recently published on the engraftment of mouse MSCs or other bone marrow cells into nonhematopoietic tissues...
  59. ncbi Versatile label free biochip for the detection of circulating tumor cells from peripheral blood in cancer patients
    Swee Jin Tan
    NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 12 Medical Drive, Singapore 117598, Singapore
    Biosens Bioelectron 26:1701-5. 2010
    ..Identification of CTCs will aid in the detection of malignancy and disease stage as well as understanding the phenotypic and genotypic expressions of cancer cells...
  60. ncbi The immunogenicity of human adipose-derived cells: temporal changes in vitro
    Kevin McIntosh
    Cognate Therapeutics, Inc, Baltimore, Maryland, USA
    Stem Cells 24:1246-53. 2006
    ..Indeed, late passage ASCs actually suppressed the MLR response. Although these results support the feasibility of allogeneic human ASC transplantation, confirmatory in vivo animal studies will be required...
  61. ncbi Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta
    Pieternella S In 't Anker
    Department of Obstetrics, Lieden University Medical Center, Leiden, The Netherlands
    Stem Cells 22:1338-45. 2004
    ..Decidua basalis and decidua parietalis are sources for maternal MSCs. The expansion potency from both fetal and maternal placenta-derived MSCs was higher compared with adult bone marrow-derived MSCs...
  62. pmc Myogenic specification of side population cells in skeletal muscle
    Atsushi Asakura
    Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, K1H 8L6 Canada
    J Cell Biol 159:123-34. 2002
  63. ncbi Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6
    Katsutoshi Ozaki
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    J Immunol 173:5361-71. 2004
    ..Finally, BXSB-Yaa mice, which develop a systemic lupus erythematosus-like disease, have greatly elevated IL-21, suggesting a role for IL-21 in the development of autoimmune disease...
  64. ncbi Isolation and characterization of cancer stem like cells in human glioblastoma cell lines
    Lei Qiang
    Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, PR China
    Cancer Lett 279:13-21. 2009
    ..Molecular and functional characterization of such a tumorigenic population may be exploited in the development of novel cancer therapeutic drugs...
  65. ncbi Pancreatic cancer stem cells
    Cheong J Lee
    Department of Surgery, University of Michigan, Ann Arbor, MI 48109, USA
    J Clin Oncol 26:2806-12. 2008
    ..Understanding the biologic behavior and the molecular pathways that regulate growth, survival, and metastasis of pancreatic CSCs will help to identify novel therapeutic approaches to treat this dismal disease...
  66. ncbi Multipotent stem cells from umbilical cord: cord is richer than blood!
    Mariane Secco
    Human Genome Research Center, Department of Genetic and Evolutive Biology, University of Sao Paulo, Rua do Matao, n 106, Cidade Universitaria, Sao Paulo, SP, CEP 05508 090, Brazil
    Stem Cells 26:146-50. 2008
  67. pmc Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations
    Jan Pruszak
    Center for Neuroregeneration Research, McLean Hospital Harvard Medical School, Belmont, Massachusetts 02478, USA
    Stem Cells 25:2257-68. 2007
  68. ncbi Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Friedrich Ebert Strasse 107, D 68167 Mannheim
    Stem Cells 22:625-34. 2004
    ..Because MSC-like cells can be isolated at high efficacy from full-term UCB donations, we regard UCB as an additional stem cell source for experimental and potentially clinical purposes...
  69. ncbi Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior
    Henry J Klassen
    Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Invest Ophthalmol Vis Sci 45:4167-73. 2004
    ..To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration...
  70. pmc Facile isolation and the characterization of human retinal stem cells
    Brenda L K Coles
    Departments of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8
    Proc Natl Acad Sci U S A 101:15772-7. 2004
    ..Their facile isolation, integration, and differentiation suggest that human RSCs eventually may be valuable in treating human retinal diseases...
  71. ncbi 1,25-dihydroxyvitamin D3 modulates Th17 polarization and interleukin-22 expression by memory T cells from patients with early rheumatoid arthritis
    E M Colin
    Erasmus MC, University Medical Center, Department of Rheumatology, Rotterdam, The Netherlands
    Arthritis Rheum 62:132-42. 2010
    ..To examine the immunologic mechanism by which 1,25-dihydroxyvitamin D(3) (1,25[OH](2)D(3)) may prevent corticosteroid-induced osteoporosis in patients with early rheumatoid arthritis (RA), with a focus on T cell biology...
  72. ncbi High efficiency of reprogramming CD34⁺ cells derived from human amniotic fluid into induced pluripotent stem cells with Oct4
    Te Liu
    International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai, China
    Stem Cells Dev 21:2322-32. 2012
    ..Besides, HuAECs were used as feeder layers to culture human iPS cells, which could not only avoid contamination with heterogeneous proteins, but also maintain iPS cells in a self-renewing and undifferentiated state for a long time...
  73. ncbi Improved synchronous production of Plasmodium falciparum gametocytes in vitro
    Quinton L Fivelman
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Mol Biochem Parasitol 154:119-23. 2007
    ..The protocol described lends itself to large scale culturing and harvesting of synchronous parasites suitable for biochemical assays, northern blots, flow cytometry, microarrays and proteomic analysis...
  74. ncbi Identification and isolation from either adult human bone marrow or G-CSF-mobilized peripheral blood of CD34(+)/CD133(+)/CXCR4(+)/ Lin(-)CD45(-) cells, featuring morphological, molecular, and phenotypic characteristics of very small embryonic-like (VSEL)
    Hanna Sovalat
    Institut de Recherche en Hématologie et Transplantation, Mulhouse, France
    Exp Hematol 39:495-505. 2011
  75. ncbi Isolation of circulating tumor cells in patients with hepatocellular carcinoma using a novel cell separation strategy
    Wen Xu
    Molecular Oncology Laboratory, and Departments of Comprehensive Treatment and Radio intervention Therapy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China
    Clin Cancer Res 17:3783-93. 2011
    ..To establish a sensitive and specific isolation and enumeration system for circulating tumor cells (CTC) in patients with hepatocellular carcinoma (HCC)...
  76. ncbi Human placenta-derived cells have mesenchymal stem/progenitor cell potential
    Yumi Fukuchi
    Division of Cellular Therapy, The Advanced Clinical Research Center, Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, 108 8639, Japan
    Stem Cells 22:649-58. 2004
    ..The placenta may prove to be a useful source of MSCs...
  77. pmc Kaposi's sarcoma associated herpes virus (KSHV) induced COX-2: a key factor in latency, inflammation, angiogenesis, cell survival and invasion
    Neelam Sharma-Walia
    H M Bligh Cancer Research Laboratories, Department of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois, United States of America
    PLoS Pathog 6:e1000777. 2010
  78. pmc Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells
    Kazim H Narsinh
    Department of Medicine, Stanford University School of Medicine, Stanford, California, USA
    J Clin Invest 121:1217-21. 2011
    ..Our results suggest that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims...
  79. pmc Ex vivo isolation and characterization of CD4(+)CD25(+) T cells with regulatory properties from human blood
    D Dieckmann
    Department of Dermatology, University of Erlangen-Nuremberg, Hartmannstrasse 14, 91052 Erlangen, Germany
    J Exp Med 193:1303-10. 2001
    ..The identification of regulatory CD4(+)CD25(+) T cells has important implications for the study of tolerance in man, notably in the context of autoimmunity, transplantation, and cancer...
  80. ncbi Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow
    Philippe Tropel
    INSERM U318, Grenoble, France
    Exp Cell Res 295:395-406. 2004
    ..Therefore, mMSCs will be an important tool to study the in vivo behaviour and fate of this cell type after grafting in mouse pathology models...
  81. pmc Isolation of renal progenitor cells from adult human kidney
    Benedetta Bussolati
    Cattedra di Nefrologia, Dipartimento di Medicina Interna, Ospedale Maggiore S Giovanni Battista, Corso Dogliotti 14, 10126 Turin, Italy
    Am J Pathol 166:545-55. 2005
    ..We propose that CD133+ cells from kidney represent a multipotent adult resident stem cell population that may contribute to the repair of renal injury...
  82. ncbi Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp
    Laura Pierdomenico
    Department of Histology, Embryology, and Applied Biology, University of Bologna, Bologna, Italy
    Transplantation 80:836-42. 2005
    ..The aim of this study was to evaluate dental pulp (DP) as a potential source of MSCs instead of bone marrow (BM)...
  83. pmc Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device
    AmirAli H Talasaz
    Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:3970-5. 2009
    ..In contrast, we could not find any circulating epithelial cells in samples from 5 healthy donors. The isolated CEpCs are all stored individually for further molecular analysis...
  84. pmc Embryonic stem cell-derived hematopoietic stem cells
    Yuan Wang
    Division of Hematology Oncology, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Division of Hematology, Brigham and Women s Hospital, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:19081-6. 2005
    ..These data document the cardinal stem cell features of self-renewal and multilineage differentiation of ESC-derived hematopoietic stem cells...
  85. ncbi Isolation of cancer stem-like cells from a side population of a human glioblastoma cell line, SK-MG-1
    Raita Fukaya
    Department of Neurosurgery, Keio University School of Medicine, Tokyo, Japan
    Cancer Lett 291:150-7. 2010
    ..Therefore, the SP cells from SK-MG-1 may be useful for analyzing BCSCs because of the ease with which they can be handled and their yield...
  86. ncbi Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells
    Marie Catherine Giarratana
    Laboratoire d Hématologie EA1638, Universite Paris VI, CHU Saint Antoine, 27 rue de Chaligny 75571 Paris Cedex 12, France
    Nat Biotechnol 23:69-74. 2005
    ..Further development of large-scale production of cultured RBCs will have implications for gene therapy, blood transfusion and tropical medicine...
  87. ncbi Purified hematopoietic stem cells can differentiate into hepatocytes in vivo
    E Lagasse
    StemCells, 525 Del Rey Avenue, Suite C, Sunnyvale, California 94085, USA
    Nat Med 6:1229-34. 2000
  88. ncbi Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source
    Yusuke Sakaguchi
    Tokyo Medical and Dental University, Japan
    Arthritis Rheum 52:2521-9. 2005
    ..To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue...
  89. ncbi A leukemic stem cell with intrinsic drug efflux capacity in acute myeloid leukemia
    G G Wulf
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA
    Blood 98:1166-73. 2001
    ..They also suggest a mechanism by which SP cells could escape the effects of cytostatic drugs and might eventually contribute to leukemia relapse. (Blood. 2001;98:1166-1173)..
  90. ncbi Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis
    Koichiro Iohara
    Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi, Japan
    Stem Cells 24:2493-503. 2006
    ..Thus, adult pulp contains SP cells, which are enriched for stem cell properties and useful for cell therapy with BMP2 for dentin regeneration...
  91. ncbi Heterogeneity of mesenchymal stromal cell preparations
    A D Ho
    Department of Medicine V, University of Heidelberg, Heidelberg, Germany
    Cytotherapy 10:320-30. 2008
  92. ncbi Mammalian neural stem cells
    F H Gage
    The Salk Institute, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 287:1433-8. 2000
    ..Before the full potential of neural stem cells can be realized, we need to learn what controls their proliferation, as well as the various pathways of differentiation available to their daughter cells...
  93. ncbi Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanoma
    Vincenzo de Giorgi
    Department of Critical Care Medicine and Surgery, Dermatology Unit, University of Florence, Florence, Italy
    J Invest Dermatol 130:2440-7. 2010
    ..001). CTC detection corroborated by suitable molecular characterization may assist in the identification and monitoring of more appropriate therapies in melanoma patients...
  94. pmc Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activity
    Simone A Joosten
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Pathog 6:e1000782. 2010
    ..Our results significantly increase our understanding of the human immune response to Mtb by identification of CD8(+) T-cell responses to novel HLA-E binding peptides of Mtb, which have cytotoxic as well as immunoregulatory activity...
  95. ncbi WNT5A is induced by inflammatory mediators in bone marrow stromal cells and regulates cytokine and chemokine production
    Martina Rauner
    Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine III, Technical University, Dresden, Germany
    J Bone Miner Res 27:575-85. 2012
    ..This study underlines the critical role of BMSC-derived WNT5A in the regulation of inflammatory processes and suggests its participation in the pathogenesis of RA...
  96. ncbi CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor
    Rebecca S McHugh
    Cellular Immunology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Immunity 16:311-23. 2002
    ..Importantly, antibodies to GITR abrogated suppression, demonstrating a functional role for this receptor in regulating the CD4(+)CD25(+) T cell subset...
  97. pmc Rare cell separation and analysis by magnetic sorting
    Maciej Zborowski
    Department of Biomedical Engineering ND 20, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, United States
    Anal Chem 83:8050-6. 2011
  98. ncbi Analysis of circulating tumor cells in patients with non-small cell lung cancer using epithelial marker-dependent and -independent approaches
    Matthew G Krebs
    Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, University of Manchester, United Kingdom
    J Thorac Oncol 7:306-15. 2012
    ..Molecular characteristics of CTCs were also explored...
  99. ncbi A new device for rapid isolation by size and characterization of rare circulating tumor cells
    Isabelle Desitter
    ScreenCell, 75012 Paris, France
    Anticancer Res 31:427-41. 2011
    ..Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells...
  100. ncbi Distinct stem cells subpopulations isolated from human adipose tissue exhibit different chondrogenic and osteogenic differentiation potential
    Tommaso Rada
    3B s Research Group Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, 4806 909 Taipas, Guimaraes, Portugal
    Stem Cell Rev 7:64-76. 2011
    ..Furthermore, the results obtained clearly evidence of the advantages of using selected populations in cell-based therapies, such as bone and cartilage regenerative medicine approaches...
  101. ncbi B cell receptor-mediated calcium signaling is impaired in B lymphocytes of type Ia patients with common variable immunodeficiency
    Christian Foerster
    Division of Rheumatology and Clinical Immunology, Centre of Chronic Immunodeficiency, University Medical Centre, Freiburg, Germany
    J Immunol 184:7305-13. 2010
    ..These data from patients with CVID suggest that a defect leading to impaired BCR-induced calcium signaling is associated with the expansion of CD21low B cells, hypogammaglobulinemia, autoimmune dysregulation, and lymphadenopathy...

Research Grants72

    David J Stillman; Fiscal Year: 2010
    ..Generally, Swi5 activates genes that promote cell cycle progression while Ace2 activates genes required for cell separation. Chromatin immunoprecipitation (ChIP) experiments show that there are two distinct mechanisms operative to ..
  2. FSH - Responsive Genes in Mouse Sertoli Cells
    T Kumar; Fiscal Year: 2005
    ..In the first approach, a rapid magnetic cell separation method will be used to selectively enrich Sertoli cells based on their unique cell surface expression of FSH-..
  3. High Performance Magnetic Cell Sorting
    JEFFREY CHALMERS; Fiscal Year: 2006
    ..This project will focus on the development/application of a high throughput, flow through immunomagnetic cell separation system currently under development for clinical scale T-cell depletion and NK isolation...
  4. Dietary Risk for Colon Cancer in the Mouse
    Leonard H Augenlicht; Fiscal Year: 2013
    ..abstract_text> ..
  5. Human hepatocytes for drug toxicity screening from Cardiac Death Donor livers
    Mark G Clemens; Fiscal Year: 2012
    ..should allow us to quickly translate this innovative technology into a commercially viable product. ..
  6. HIV and CD16+ monocytes as cellular reservoir &HIV/pulmonary hypertension
    Sharilyn Almodovar; Fiscal Year: 2013
    ..We will use several laboratory techniques single-genome amplification, phylogenetic analyses, cell isolation using magnetic beads, fluorescent in itu hybridization and in vivo cell imaging. ..
  7. Stem Cell Based Therapy for Regenerative Endodontics
    George T J Huang; Fiscal Year: 2013
    ..If successful, a subsequent human trial may be carried out in the future and its success will promise a reduced need of traditional aggressive root canal therapies. ..
  8. High Efficiency Microfluidic Purification of Stem Cells to Improve Transplants
    James C Sturm; Fiscal Year: 2012
    ..depend on the numbers of leukocytes and HSPCs per recipient body weight, it is essential to develop new cell separation methods to ensure high yields of pure, viable leukocytes and HSPCs from harvested UCB...
  9. A Novel Therapy for Radiation-Induced Xerostomia Using Human Salivary Stem Cells
    QUYNH THU XUAN LE; Fiscal Year: 2012
    ..These studies will support future use of SMG stem cells for salivary function restoration in irradiated HNC patients. ..
  10. Changes in antioxidants and susceptibility to parasites
    DEBORAH KRISTAN; Fiscal Year: 2012
  11. Role of CaMKII Phosphorylation of RyR in Pathological &Physiological Hypertrophy
    Jonathan L Respress; Fiscal Year: 2011
    ..Abnormal Ca2+ regulation in the heart muscle cell could lead to development of HP. In this project, we will use a genetic approach to test whether inhibition of Ca2+ leak leads to prevention of hypertrophy and heart failure development. ..
  12. Structure and Function of Endothelial Fenestrae
    RADU VIRGIL STAN; Fiscal Year: 2010
    ..The techniques employed are cell isolation, fractionation, biochemical assays, cell free-assays, genetically modified animal models, cell culture, transfections, light and electron microscopy. ..
  13. Nano-sized Cell Guidance System for Ischemic Tissue Repair
    Hyunjoon Kong; Fiscal Year: 2010
    ..In the end, this study will aid saving a number of patients who suffer from the ischemic disorders of myocardial and peripheral tissues. ..
  14. Use of Umbilical Core Blood derived HSC to Treat Acute Kidney Injury
    Fangming Lin; Fiscal Year: 2010
    ..Stem cells offer therapeutic potential for kidney disease. The goal of this application is to develop cell therapeutic agents from human umbilical cord blood-derived stem cells to treat acute kidney injury. ..
  15. Role of BMPs in Adipogenesis, Mitochondrial Function and Energy Metabolism
    Yu Hua Tseng; Fiscal Year: 2013
  16. Peptidoglycan Biogenesis in Escherichia Coli
    Thomas G Bernhardt; Fiscal Year: 2013
    ..of the PG hydrolases (amidases) AmiA and AmiB that stimulates their activity to bring about daughter cell separation during cytokinesis...
  17. Biocompatibility of Implantable Renal Replacement Devices
    Shuvo Roy; Fiscal Year: 2013
  18. Roles of the Unfolded Protein Response in Cell Cycle
    MAHO R NIWA; Fiscal Year: 2013
    ..dynamics of the septin complex, which normally assembles at the mother/daughter cell bud neck and mediates cell separation during cytokinesis...
  19. Development of a Novel TGF-beta Antagonist for Treating Hepatic Fibrosis
    Jung S Huang; Fiscal Year: 2010
    ..The novel TGF-B receptor antagonist developed in this project should be an ideal drug candidate for treating HF in humans. ..
    Weimin Zhong; Fiscal Year: 2010
  21. 1st International Symposium on Childhood, Adolescent and Young Adult Hodgkin Lymp
    Monika Metzger; Fiscal Year: 2011
  22. Autogeneic Mitochondria: Surgical Cardioprotection
    James D McCully; Fiscal Year: 2013
  23. Anti-PV1 Therapy for Pancreatic Cancer
    RADU VIRGIL STAN; Fiscal Year: 2013
    ..In summary, the data we expect to obtain are relevant to the pathophysiology of cancer, establishing PV1 as a novel target for cancer treatment, which is one of NCI's stated goals. ..
  24. TLR4 signaling in the pathogenesis of surgical necrotizing enterocolitis
    David J Hackam; Fiscal Year: 2013
  25. Molecular Evolution of Multifunctional DNA Nanoparticles
    Bradley T Messmer; Fiscal Year: 2013
    ..Particles tagged with biotin or iron oxide will used for magnetic cell separation. Aim 3. Identify the molecular targets of the cancer cell specific DNA nanoparticles...
  26. Adult Stem Cell Minimally Invasive Bone Marrow Harvester
    DANIEL LEWIS KRAFT; Fiscal Year: 2012
    ..Most of the research and investment dollars to date have been spent on stem cell separation and especially delivery...
  27. An Integrated Approach to Airway Epithelial Repair and Regeneration
    Barry R Stripp; Fiscal Year: 2013
    ..The group brings unique skills and resources to the Consortium that will accelerate discovery of new therapies for lung diseases afflicting millions of people...
  28. Training in the Molecular Basis of Skin/Mucosa Parthobiology
    Molly F Kulesz-Martin; Fiscal Year: 2013
    ..5 million people in the US annually, ..
  29. Magnetophoretic Cell Sorting and Analysis
    Maciej Zborowski; Fiscal Year: 2013
    ..We propose to investigate the feasibility of extending the current magnetic cell separation methods to applications relying on the cell's own, natural (intrinsic) magnetization...
  30. Development of an adipose-derived stem cell isolation matrix
    MARTYN DARBY; Fiscal Year: 2010
    ..We currently envision this peptide-based reagent to be used in a culture-free method of ASC isolation prior to their use in tissue engineering applications. ..
  31. Mitochondrial genetics in skeletal muscle aging
    Jonathan Wanagat; Fiscal Year: 2013
    ..Additionally, this Career Development Award will support the unique career of a physician-scientist working in the areas of gerontology and geriatric medicine, for which there is a growing public health need. ..
  32. Multi-Dimensional Separation of Bacteria
    G Scott Worthen; Fiscal Year: 2010
    ..We also are interested in contributing to an understanding of how tools such as fluid dynamics (for which a new program of numerical simulation will be presented to the scientific community) and microfluidics intersect with medicine. ..
  33. Role of Proteasome Inhibition in the Pathogenesis of CADASIL
    Michael M Wang; Fiscal Year: 2010
    ..Finally, proteasome inhibition in cultured smooth muscle cells causes cell-cell separation and cell death, two features seen in CADASIL tissues...
  34. The Early Detection Research Network: Biomarker Developmental Laboratories
    Alvin Y Liu; Fiscal Year: 2013
    ..These can be applied to diagnose tumors for the presence of specific cancer cell types, which will then provide prognostic information. ..
  35. A Compartmental Analysis of HIV Reservoirs and Immune Reconstitution
    Timothy W Schacker; Fiscal Year: 2012
    ..Dr. Douek will perform flow cytometry to measure antigen specific responses and cell separation and culture virus from CD4 cells obtained from each compartment before and during treatment interruption...
  36. Comparative transcriptome of brain blood vessels: age, hypertension and diabetes
    Eng H Lo; Fiscal Year: 2010
    ..And we will also find out how aging, hypertension and diabetes alter these gene patterns. This database should be invaluable for stroke research, in terms of finding new therapeutic targets in the future. ..
  37. Immune Response to pneumococcal vaccination in HIV infected adults
    M A Westerink; Fiscal Year: 2009
    ..abstract_text> ..
  38. Alveolar Bone Marrow Derived Stem Cells for Clinical Cell Therapy
    Darnell Kaigler; Fiscal Year: 2013
    ..Additionally, the isolation of aBMSCs with the technique employed would have broad scientific impact in providing an alternative source of MSCs to be used in fundamental investigations of stem cell and bone biology. ..
  39. Role of SNARE proteins in HIV-1 assembly and release
    Anjali Joshi; Fiscal Year: 2013
    ..These include daughter cell separation during cytokinesis, a phenomenon that bears striking similarity to the process of HIV-1 budding...
  40. Inflammation and the Host Responses to Injury
    RONALD GARY TOMPKINS; Fiscal Year: 2010
    ..including microarray and comparative, quantitative proteomics coupled with novel macroscale and microfluidics cell separation methodologies and bioinformatics approaches (including knowledge-based pathway analysis)...
  41. Normal and Cancer Stem Cells of the Mammary Gland
    Jonathan A Cooper; Fiscal Year: 2012
    ..These studies represent a new and promising paradigm for breast cancer research. ..
  42. Host-Oriented Therapeutics Targeting Filovirus Budding.
    Ronald N Harty; Fiscal Year: 2013
    ..As L-domain containing matrix proteins are required for efficient virus-cell separation of many RNA viruses, including retroviruses, arenaviruses, rhabdoviruses, paramyxoviruses, henipaviruses, and ..
  43. Immune Response to pneumococcal vaccination in HIV infected adults
    M A JULIA WESTERINK; Fiscal Year: 2013
    ..More importantly, the results of these studies could clarify controversies in the present vaccine recommendations in the HIV-infected population. ..
  44. Cell separation in Neisseria gonorrhoeae
    Joseph P Dillard; Fiscal Year: 2013
    ..This proposal seeks to understand the mechanisms of peptidoglycan breakdown involved in cell separation and to develop cell separation as a target for antimicrobial therapy...
  45. A mouse model for genetic tracing to study stress responses
    Tsonwin Hai; Fiscal Year: 2013
    ..The broad inducibility of Cre* makes the model an excellent tool to study environmentally-induced diseases - a special emphasis of NIEHS. ..
    Hidekazu Tsukamoto; Fiscal Year: 2013
    ..New information generated by these studies with support from the core, will lead to identification of new treatment modalities for the diseases. ..
  47. Cell-Specific Transcription in Cancer Microenvironment in vitro and in vivo
    Richard A Steinman; Fiscal Year: 2013
    ..protozoan enzyme (UPRT) to enable cell-type specific transcript labeling without requiring microdissection or cell separation. Global reprogramming of stromal cells during direct contact with highly bone-metastatic and less metastatic ..
  48. Point of Care Adult Stem Cell Isolation
    MARTYN DARBY; Fiscal Year: 2012
    ..This product will provide a safe and cost-effective point-of-care isolation system for the preparation of autologous cells. ..
  49. Arrays for Cloning Growth Suppressed Cells
    Nancy L Allbritton; Fiscal Year: 2013
    ..The ability to isolate and grow cells carrying such genes will enhance our understanding of the importance of the molecular basis of tumor suppressor genes in cancer. ..
    Timothy L Ratliff; Fiscal Year: 2013
    ..The four scientific programs are served by seven shared resources: Flow Cytometry and Cell Separation;DNA Sequencing;Proteomics;Macromolecular Crystallography;Mass Spectrometry;Nuclear Magnetic Resonance;and ..
  51. Cell Based Therapy for Lung Disease
    Carl W White; Fiscal Year: 2010
  52. Preclinical Investigation of a Bioengineered Vascular Graft
    MELINA RAE KIBBE; Fiscal Year: 2013
    ..This proposal will allow us to gather the necessary expertise and data to design a clinical trial to assess early stage efficacy of the bioengineered grafts. ..
  53. IPF Fibroblast Phenotype
    Craig A Henke; Fiscal Year: 2013
    ..A major objective of this Program Project is to inform decisions of the IPF Clinical Network by providing information that can be translated into novel therapeutic strategies for IPF. ..
    Erik J Woods; Fiscal Year: 2010
  55. COBRE in Stem Cell Biology and Regenerative Medicine
    Don Wojchowski; Fiscal Year: 2012
    ..components;acquired several R01 and K01 awards;made major infrastructural advances (eg, new cores in cell separation, histopathology, bioinformatics and a new research building addition);and have extended intra-inter Institute ..
  56. Impaired AMPK activity contributes to vascular pathogenesis associated with PH
    Judy R Creighton; Fiscal Year: 2013
    ..2] Changes in AMPK ????? expression patterns correlate with segment specific vascular disease in PH. ..
  57. Reversing age-related dysfunction of skeletal muscle stem cells
    AMY JO WAGERS; Fiscal Year: 2013
    ..abstract_text> ..
  58. Collaborative Approaches to the Study of Intestinal Epithelial Stem Cells
    Susan J Henning; Fiscal Year: 2013
    ..Moreover, in situations where the stem cells themselves are damaged (e.g. after radiation or chemotherapy), transplantation of healthy stem cells may afford a novel and effective therapy. ..
  59. GUDMAP2 - Production of Mouse Strains for Gene Anatomy of the Lower Urinary Tract
    Andrew P McMahon; Fiscal Year: 2013
    ..These strains will be distributed to GUDMAP II members to facilitate their analyses and to NIH-funded Mouse Mutant Resource Centers for distribution to the biomedical research community. ..
  60. Commercialization of Magnetic Nanobeads for Cancer Cell Enrichment
    Arfaan Rampersaud; Fiscal Year: 2009
    ..b>Cell separation is becoming increasingly commonplace for researchers and clinicians who isolate rare cells from complex cell ..
  61. MicroRNA Profile in Peripheral Artery Disease
    NAOMI MIRIAM HAMBURG; Fiscal Year: 2013
    ..We relate endothelial cell miRNA expression to circulating miRNAs and PAD status. These studies will provide new information regarding circulating and endothelial miRNAs in PAD and may stimulate innovative treatment strategies. ..
  62. LEAP Image Scanning Cytometer/Sorter/Optoinjection Shared Instrument
    James F Leary; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The Purdue Flow Cytometry and Cell Separation Facility of the Bindley Biosciences Center in Discovery Park at Purdue University proposes to purchase a new LEAP (Laser Enabled Analysis and Processing) ..
  63. Development of an adipose-derived stem cell isolation matrix
    MARTYN DARBY; Fiscal Year: 2013
    ..In this phase 2 proposal, we will develop a point-of-care autologous ASC enrichment system for bone grafting that eliminates the need for ex vivo cell expansion prior to implantation. ..
  64. Microfluidic Cell Separation for Tissue Engineering and Regenerative Medicine
    SHASHI MURTHY; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The major goal of this proposal is to create microfluidic cell separation systems to isolate or enrich key cell types for tissue engineering and regenerative medicine...
  65. A Novel Method to Isolate Islet Cells
    JERRY KNOBELOCH; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
    David Sullivan; Fiscal Year: 1993
    ALLEN COOPER; Fiscal Year: 2003
    ..As a back up strategy, cell separation techniques can be used to accomplish the goals of the first two methods...
  68. Generation of therapeutic T cells from cord blood-derived stem cells
    Krishnendu Roy; Fiscal Year: 2009
    ..We would also investigate if tetramer signaling through magnetic microbeads (artificial antigen presenting cells) can further differentiate these stem cell-derived early T cells into more mature, CD8+ antigen specific T cells. ..
  69. Mechanical Induced Resonant Mixing for Islet Separation
    FANGXIAO YANG; Fiscal Year: 2004
    The proposed research is aimed to develop a mechanically-induced resonant mixing process for pancreas islet cell separation. The proposed study incorporates a unique mixing technology into a Resonant Enhanced Enzyme Tissue Digester (..
  70. Chondroprogenitor cells in mature cartilage
    Martin Lotz; Fiscal Year: 2006
    Donald Schauwecker; Fiscal Year: 1993
    ..Several methods exist for isolating white blood cells which differ considerably in the complexity of cell separation and the final cell purity...
  72. High Throughput Rare Cell Isolation System
    CAROLYN CONANT; Fiscal Year: 2009
    ..This proposal aims to develop a high throughput system to isolate rare cells from biological samples and provide a range of analysis options to accommodate the desired endpoints of both clinical and research applications. ..