freeze drying

..It was concluded that enhanced serum and mucosal antibody responses were elicited by a dry powder nasal vaccine, specifically, administered in the presence of sodium alginate...

The purpose of this study is to investigate whether spray freeze drying produces an inhalable solid dispersion powder in which Delta(9)-tetrahydrocannabinol (THC) is stabilised...

..ESEM analysis was carried out to monitor morphology of the rehydrated liposomes...

..To investigate the impact of drying method on the storage stability of dried vaccine formulations...

..This research represents a significant step towards the development of measles vaccine formulation that is effective, temperature-stable, easy to administer in a resource-poor setting and amenable to large scale manufacture...

..We propose that residual moisture is followed during lyophilization of bacteriophage from minimal concentrations of bulking agent...

..This study describes the preparation and pharmacokinetics of docetaxel based on freeze-dried nanostructured lipid carriers (NLCs)...

..The ABCB1 purification procedure was optimised to allow successful freeze drying by substitution of glycerol with the disaccharides trehalose or maltose...

..lipid concentration range for lyophilization of sterically stabilized phospholipid nanomicelles (SSM) and the freeze drying feasibility of self-associated therapeutic peptide-SSM assemblies...

..Our results help explain how RL platelets restore hemostasis in vivo, and indicate that these cells might be a viable alternative to fresh stored platelets in transfusion medicine...

..The initial burst was dependent on the drug loading and could be significantly reduced by wet (non-aqueous) temperature curing...

..The rate of water sorption can be rank ordered as trehalose prepared by dehydration < freeze-dried < spray-dried...

..Furthermore, the storage of lyophilized lipoplexes stabilized with the disaccharide trehalose did not affect cell viability...

..Collectively, our data indicate that the fructo-oligosaccharides accumulated in many plant species under stress conditions could indeed play an important role in cellular dehydration tolerance...

..In conclusion, Raman spectroscopy is a potentially useful technique to monitor physical changes during freeze-drying...

Spray freeze drying produces protein particles suitable for microencapsulation into polymeric microspheres intended for sustained release...

..For the development of lyophilization cycles the lowered Tg', as well as the tendency to mannitol hydrate formation predominantly at lower temperature needs to be considered...

..W., 2008. A novel bottom-up process to produce drug nanocrystals: controlled crystallization during freeze drying. J. Control. Release 128, 179-183]...

..The modified process with a single lyophilization step is a more practical process and can be utlized to prepare cationic PLG microparticles with adsorbed DNA on a large scale...

..Overall, these results allow for the use of freeze-dried NPs in upcoming preclinical trials of the different squalenoylated compounds developed in our laboratory...

..At midterm follow-up, there was no aseptic loosening. In this limited case report, the results at midterm for freeze-dried allograft bone chips in acetabular reconstructions are acceptable...

..It can be carried out with a balance and a freeze-dryer, and has the additional advantage that a large number of candidate formulations can be evaluated simultaneously...

..Compared to dextran, inulin was also a superior lyoprotectant of PEGylated lipoplexes: during freeze drying and subsequent storage at 37 degrees C of the dried powders for 3 months the PEGylated lipoplexes maintained ..

..Raman spectroscopy (in-line) and NIR spectroscopy and XRPD (at-line) not only allowed the real-time monitoring of mannitol freeze-drying processes but also helped (in combination with experimental design) us to understand the process...

In the present study, the effect of irradiation, storage, and freeze drying on grapefruit bioactive compounds was investigated...

..e. lyophilization, spray drying, spray-freeze drying, vacuum drying or supercritical fluid drying), the current status of dry influenza vaccine development and the ..

To improve the dissolution behavior of lipophilic drugs, a novel bottom-up process based upon freeze drying which allows for the production of nanocrystalline particles was developed: "controlled crystallization during freeze drying&..

..More important, this study led to the selection of a formulation system that had been successfully tested in a human clinical study...

This study investigates the feasibility of using the process of spray-freeze drying (SFD) to produce DNA dry powders for non-viral gene delivery. The effect of protective agents was assessed on the stability of DNA dry powders after SFD...

..It is concluded that freeze-drying in the presence of inulin as a lyoprotectant completely preserves the structure and function of influenza virosomes...

..Herein, the freeze drying process of nanocrystal dispersions was examined at varying freezing rates (speed of freezing interface)...

..Freeze-dried grafts provide more stable fixation of the stem than fresh-frozen morselized grafts, when tested in a hip simulator...

..coated plasmid DNA/cationic liposome complexes ("PEGylated lipoplexes") during freeze thawing and freeze drying [Hinrichs et al., 2005. J. Control. Release 103, 465]. By contrast, dextran clearly failed as stabilizer...

..Vaccine powder was produced by spray-freeze drying (SFD) using the oligosaccharide inulin as stabilizer...

..0 and pH 9.8. Also, the glass transition temperature (Tg) of sugar glasses obtained after freeze drying of these solutions was elucidated...

..Further optimization of particle size and morphology is required for these powders to be suitable for clinical evaluation...

..These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine and for conservation strategies...

..Both formulations provided adequate stability for the adenovirus, with 2.6 and 5.6 x 10(11) lfu/ml detected 150 days after drying, respectively...

..We have investigated the structural consequences of moisture sorption by lyophilized insulin under controlled humidity conditions by employing Fourier transform-infrared (FT-IR) microscopy...

..This can be understood because the dehydration and not the freezing process, as shown in an air-drying experiment, mainly causes protein structural alterations...

..Formulation of influenza virosomes as a dry-powder is advantageous for storage and transport and offers the possibility to develop needle-free dosage forms, e.g. for oral, nasal, pulmonal, or dermal delivery...

..The half-life of solute retention in dry liposomes may be prolonged by storing dry liposomes at temperatures below the Tg and by increasing the Tg of the dry liposome preparation...

To develop a new technique, spray freeze drying, for preparing protein aerosol powders. Also, to compare the spray freeze-dried powders with spray-dried powders in terms of physical properties and aerosol performance.

..The mobility of protein in powders at different hydration levels was studied in relation to aggregation and activity...

..For mannitol and sucrose, however, the level of inactivation was higher than could be explained by surface inactivation alone, and additional mechanisms must also be considered...

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..To investigate the effect of atomization conditions on particle size and stability of spray-freeze dried protein...

..Moreover, the HA potency of the influenza subunit vaccine powder was stable for at least 26 weeks at room temperature...

..When freeze drying is envisaged as method of drying, a relatively high Tg of the freeze concentrated fraction (Tg') is ..

..The results of impaction bone grafting with freeze-dried bone alone have been satisfactory, although we recommend caution in its use alone in the hostile acetabulum...

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..In addition, the application of this new method to the passive loading and active loading of drugs into liposomes is discussed in detail...

..The acceleration of sublimation rate seems to depend on the temperature of annealing. The annealing of sucrose solution slows down the secondary drying kinetic whereas no effect is observed in the case of PVP...

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..We suggest that this finding will obviate the storage problem with platelets and that the principles established here may be extended to more complex eukaryotic cells...

..2 g H2O/g dry weight, as measured by gravimetric analysis. These results indicate that p26 improves cell survival following drying and rehydration, and suggest that dry storage of mammalian cells is a likely possibility in the future...

..We review the available mechanisms for introducing trehalose into the cytoplasm of living cells as an introduction to the papers that follow...

..Imexon 100 mg/vial lyophilised product was shown stable for at least 12 months of storage at -20 degrees C and +5 +/- 3 degrees C in the dark...

..In vitro release studies showed that the dissolution rate of nanosuspension, compared with micronized powder, had been obviously increased...

..Thus, different mass transport mechanisms govern the release of the drug, co-lyophilisation agent and release modifier out of the lipidic implants...

..Addition of the antioxidant sodium metabisulfite increased the stability of lycopene-HP-beta-CD binary system in water. At -20 degrees C, the lyophilized lycopene-cyclodextrin binary systems were stable for at least 2 weeks...

..The drug is currently used in phase I clinical trials...

..8%) was investigated after three processes: freeze-thawing, freeze-drying, and storage at three temperatures (20, 40, and 60 degrees C) above and/or below the glass transition temperature (T(g))...

..The results demonstrate the importance of understanding effect of excipient ratios, specifically in systems containing crystallizing and non-crystallizing excipients, and thermal history when developing freeze-dried formulations...

..This study demonstrates the usefulness of TSDC in the analysis of lot-to-lot variations in amorphous pharmaceuticals...

..The glassy character of these formulations as well as their high collapse temperature values (-9 and -12 degrees C, respectively) should allow simplification and shortening of freeze-drying process...

..Experimental results confirm that an inclusion complex exists in the solid state and possible structure of the complex is briefly discussed...

..Water may provide a favourable environment for degradation. These findings, taken together, should be considered during the selection of excipients for a possible formulation of gabapentin and baclofen...

..Solid-state NMR spectroscopy was shown to be a powerful technique for the analysis of drug formulations and investigations of the effects of processing conditions...

..Fast releasing indomethacin microparticles were prepared encapsulating co-freeze-dried indomethacin/poly(vinylpyrrolidone) particles (IMC/PVP) into molten stearic acid (SA), by means of a ultrasonic spray-congealing technique...

..The main objective of the study was to formulate and characterize testosterone (TS) solid lipid microparticles (SLM) to be applied as a transdermal delivery system...

..Microparticles prepared by spray-drying method had a slower release compared to those of casting-drying method. Spray-drying method seems to be a more suitable method to prepare microparticles for prolongation in release...

..In addition to increasing aqueous solubility, a nanosized compound/carrageenan complex had a faster dissolution rate than the complex, the free compound, and the nanosuspension of the free compound...

..6% Pfizer product) and polyvinylpyrrolidone (40 kDa) likely due to water "re-adsorption" by the amorphous product during the MTM test...

..The physicochemical properties of the pure drug and the corresponding products prepared by two different SCF methods were also compared...

..5-8.5 h) relative to the faster drug release from carriers that contained no filler (3.5 h). In summary, alginate, agar, and gellan beads with filler inclusion may be useful for slow drug release...

..Both the voidage and dissolution characteristics are postulated to be determined by the microstructure established during drying...

..However, the large zeta potential associated with the suspensions suggested that charge stabilisation may also influence the pMDI physical stability...

..Therefore, trehalose and HA offer complementary properties that improve the stability of PKase during dehydration, storage and rehydration...

..This variety in cryoprotective ability of saccharide species can be attributed to differences in their interaction with the E5531 head group...

..Solutions of Met-hGH with different stabilizers were dried by different methods (freeze drying, spray drying, and film drying), with and without surfactant...

..Despite secondary structural changes, freeze-dried protein in the presence and absence of amino acids refolded back into its native conformation upon reconstitution in water...

..0002025 + 20.23l, is similar to that obtained by Pikal [Pikal, M.J., 1985. Use of laboratory data in freeze drying process design: heat and product resistance parameters and the compute simulation of freeze drying. J. Parent...

..Generally, 95% of the incorporated protein was delivered continuously within 1 month in monomeric form, even after 6 months' storage of the implants at room temperature...

..Here the benefit of NaCl on HSA stability outbalances the potential drawbacks that morphological changes, like crystallization impose on protein stability in the lyophilized products...

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..The present study demonstrates the synergistic effect of combining two types of stabilizers, PVP and CAR, on the stability of amorphous drug during compression and storage as compared to their effect when used alone...

..Finally, secondary drying kinetics were studied by measuring the values of the desorption constant by a simple gravimetric method. In this study, annealing turned out to decrease desorption kinetics by a factor of two...

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..These modifications, and the resulting aggregation of Alhydrogel particles can be minimized through choice of buffer ions, or kinetically inhibited by rapidly forming a glassy state during freezing...

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..1 cells was over 4-fold higher than that of the CoQ10-free nanoparticles (P < .05). In conclusion, CoQ10-NPs with potential application for oral CoQ10 delivery were engineered readily from microemulsion precursors...

..Dioscorea starches were pregelatinized followed by either oven drying (PS) or freeze drying (FD) and used as excipient in direct compression...

..The results indicate that the choice of lyophilisation excipient for aziridinylbenzoquinones cannot be based on previous literature studies of related agents...

..The possibility of predicting storage stability based on the temperature dependence of the estimated parameters of inactivation/aggregation--time constant (tau) and its distribution (beta) is discussed...

..The results are consistent with an osmotically driven release mechanism...

..The often discussed failure of dextran as a stabilizing excipient in the freeze-drying of proteins seems to be a question of the selection of the correct molecular weight...

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..Vaccine campaigns have failed to eliminate measles because the vaccine goes bad when it is heated. Our project is to make a new vaccine that will not lose its ability to protect people if there is no refrigeration. ..

..are treated with various chemicals (detergents, DTT, exogenous DNA) or are subjected to mechanical insults (freeze drying and freezing without cryoprotection), and then injected into the oocytes, the paternal chromosomes in the ..

..Phase II studies will be performed to develop formulations and methods that permit storage at refrigeration or ambient temperatures and scale-up the resulting freeze-drying technology to sheep and human heart valves. ..

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..Vaccine campaigns have failed to eliminate measles because the vaccine goes bad when it is heated. Our project is to make a new vaccine that will not lose its ability to protect people if there is no refrigeration. ..

..The overall result will be a healthier life for people with diabetes and lower medical costs for diabetes care for the society. ..

..The third specific aim will optimize fermentation and freeze drying conditions to produce a product with satisfactory cost, efficacy, safety and shelf life...

..In addition to designing excipient formulations to stabilize lyophilized preparations, these studies will help to better characterize the "native state" of synthetic gene delivery systems. ..

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..We believe the novel delivery approach of this vaccine has potential to provide a broad immune response more capable of decreasing if not preventing tumor burden. ..

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..loading methodologies, from endocytotic to peptide-mediated, will be evaluated for their applicability for pre-freeze drying loading...

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..g., lipoplexes and polyplexes) in order to develop rational formulation guidelines that are generally applicable to the stabilization of macromolecular complexes (e.g., vaccines, viruses, nanoparticles) during freeze-drying and storage. ..

..g., vaccines, viruses, nanoparticles) during freeze-drying and storage. ..

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..The objectives of the studies, the hypothesis upon which the experimental design is based, and a timeline for completion of the studies are presented. ..