freeze drying

Summary

Summary: Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.

Top Publications

  1. ncbi Novel dry powder preparations of whole inactivated influenza virus for nasal vaccination
    Robert J Garmise
    School of Pharmacy, University of North Carolina at Chapel Hill, CB 7360 Kerr Hall Room 1310, Chapel Hill, NC 27599, USA
    AAPS PharmSciTech 8:E81. 2007
  2. ncbi Drying-induced variations in physico-chemical properties of amorphous pharmaceuticals and their impact on Stability II: stability of a vaccine
    Ahmad M Abdul-Fattah
    Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA
    Pharm Res 24:715-27. 2007
  3. doi Stabilization of bacteriophage during freeze drying
    U Puapermpoonsiri
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Strathclyde G4 0NR, UK
    Int J Pharm 389:168-75. 2010
  4. doi Effect of culture medium and cryoprotectants on the growth and survival of probiotic lactobacilli during freeze drying
    A Siaterlis
    Department of Food Biosciences, The University of Reading, Berkshire, Reading, UK
    Lett Appl Microbiol 48:295-301. 2009
  5. doi An overview of liposome lyophilization and its future potential
    Chengjun Chen
    College of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road, No 103, Shenyang, 110016, PR China
    J Control Release 142:299-311. 2010
  6. ncbi The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state
    J H Crowe
    Biostabilization Program, University of California, Davis, CA 95616, USA
    Cryobiology 43:89-105. 2001
  7. doi Development of a lyophilized plasmid/LPEI polyplex formulation with long-term stability--A step closer from promising technology to application
    Julia Christina Kasper
    Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig Maximilians University, Butenandtstrasse 5, 81377 Munich, Germany
    J Control Release 151:246-55. 2011
  8. ncbi Measles virus hemagglutinin protein expressed in transgenic lettuce induces neutralising antibodies in mice following mucosal vaccination
    D E Webster
    The Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne, and School of Biological Sciences, Department of Immunology, Monash University, Vic, Australia
    Vaccine 24:3538-44. 2006
  9. doi Application of different methods to formulate PEG-liposomes of oxaliplatin: evaluation in vitro and in vivo
    Sara Zalba
    Department of Pharmacy and Pharmaceutical Technology, University of Navarra, Pamplona, Spain
    Eur J Pharm Biopharm 81:273-80. 2012
  10. doi Characterisation of freeze-dried wafers and solvent evaporated films as potential drug delivery systems to mucosal surfaces
    Joshua S Boateng
    Department of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, UK
    Int J Pharm 389:24-31. 2010

Research Grants

  1. A Thermostable Measles Vaccine
    Jeffrey Griffiths; Fiscal Year: 2009
  2. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
  3. Freeze-Dried, Viable Allogeneic Heart Valves
    Kelvin Brockbank; Fiscal Year: 2006
  4. Stable Micronized Vaccines Against Smallpox and Japanese Encephalitis
    VICTOR BRONSHTEIN; Fiscal Year: 2007
  5. Novel Osteoinductive Protein Synthesizing Implant System
    Kyumin Whang; Fiscal Year: 2003
  6. Revitalizing Allografts for Craniofacial Reconstruction
    Edward Schwarz; Fiscal Year: 2007
  7. A Thermostable Measles Vaccine
    Jeffrey K Griffiths; Fiscal Year: 2010
  8. Fluorescence Sensor Probe for Transcutaneous Glucose Sensing
    Ralph Ballerstadt; Fiscal Year: 2007
  9. DIGESTION OF FOOD OXALATE
    Harmeet Sidhu; Fiscal Year: 2002
  10. LYOPHILIZATION OF NONVIRAL GENE DELIVERY SYSTEMS
    THOMAS ANCHORDOQUY; Fiscal Year: 2002

Detail Information

Publications321 found, 100 shown here

  1. ncbi Novel dry powder preparations of whole inactivated influenza virus for nasal vaccination
    Robert J Garmise
    School of Pharmacy, University of North Carolina at Chapel Hill, CB 7360 Kerr Hall Room 1310, Chapel Hill, NC 27599, USA
    AAPS PharmSciTech 8:E81. 2007
    ..It was concluded that enhanced serum and mucosal antibody responses were elicited by a dry powder nasal vaccine, specifically, administered in the presence of sodium alginate...
  2. ncbi Drying-induced variations in physico-chemical properties of amorphous pharmaceuticals and their impact on Stability II: stability of a vaccine
    Ahmad M Abdul-Fattah
    Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA
    Pharm Res 24:715-27. 2007
    ..To investigate the impact of drying method on the storage stability of dried vaccine formulations...
  3. doi Stabilization of bacteriophage during freeze drying
    U Puapermpoonsiri
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Strathclyde G4 0NR, UK
    Int J Pharm 389:168-75. 2010
    ..We propose that residual moisture is followed during lyophilization of bacteriophage from minimal concentrations of bulking agent...
  4. doi Effect of culture medium and cryoprotectants on the growth and survival of probiotic lactobacilli during freeze drying
    A Siaterlis
    Department of Food Biosciences, The University of Reading, Berkshire, Reading, UK
    Lett Appl Microbiol 48:295-301. 2009
    To investigate the effects of the medium and cryoprotective agents used on the growth and survival of Lactobacillus plantarum and Lactobacillus rhamnosus GG during freeze drying.
  5. doi An overview of liposome lyophilization and its future potential
    Chengjun Chen
    College of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road, No 103, Shenyang, 110016, PR China
    J Control Release 142:299-311. 2010
    ..Finally, the major areas and future potential of research on lyophilized liposomes are highlighted...
  6. ncbi The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state
    J H Crowe
    Biostabilization Program, University of California, Davis, CA 95616, USA
    Cryobiology 43:89-105. 2001
    ..We review the available mechanisms for introducing trehalose into the cytoplasm of living cells as an introduction to the papers that follow...
  7. doi Development of a lyophilized plasmid/LPEI polyplex formulation with long-term stability--A step closer from promising technology to application
    Julia Christina Kasper
    Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig Maximilians University, Butenandtstrasse 5, 81377 Munich, Germany
    J Control Release 151:246-55. 2011
    ..All samples were amorphous with low residual moisture levels (<1.3%) and high glass transition temperatures (>90°C)...
  8. ncbi Measles virus hemagglutinin protein expressed in transgenic lettuce induces neutralising antibodies in mice following mucosal vaccination
    D E Webster
    The Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne, and School of Biological Sciences, Department of Immunology, Monash University, Vic, Australia
    Vaccine 24:3538-44. 2006
    ..This research represents a significant step towards the development of measles vaccine formulation that is effective, temperature-stable, easy to administer in a resource-poor setting and amenable to large scale manufacture...
  9. doi Application of different methods to formulate PEG-liposomes of oxaliplatin: evaluation in vitro and in vivo
    Sara Zalba
    Department of Pharmacy and Pharmaceutical Technology, University of Navarra, Pamplona, Spain
    Eur J Pharm Biopharm 81:273-80. 2012
    ..In conclusion, the FM was the simplest method in comparison with REV and HM to develop in vivo stable and efficient PEG-coated liposomes of oxaliplatin with a loading higher than those reported for REV...
  10. doi Characterisation of freeze-dried wafers and solvent evaporated films as potential drug delivery systems to mucosal surfaces
    Joshua S Boateng
    Department of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, UK
    Int J Pharm 389:24-31. 2010
    ....
  11. ncbi A pilot study of freeze drying of poly(epsilon-caprolactone) nanocapsules stabilized by poly(vinyl alcohol): formulation and process optimization
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés UMR CNRS 5007, CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France
    Int J Pharm 309:178-88. 2006
    ..Therefore, freeze drying of these colloidal systems is an alternative method to achieve long-term stability...
  12. doi In vitro drug release studies of polymeric freeze-dried wafers and solvent-cast films using paracetamol as a model soluble drug
    Joshua S Boateng
    Department of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, UK
    Int J Pharm 378:66-72. 2009
    ..The wafers which formed a porous network, hydrated faster than the more dense and continuous, (non-porous) sheet-like structure of the films...
  13. pmc Freeze drying of peptide drugs self-associated with long-circulating, biocompatible and biodegradable sterically stabilized phospholipid nanomicelles
    Sok Bee Lim
    Department of Biopharmaceutical Sciences, University of Illinois at Chicago, Chicago, IL 60612 7231, USA
    Int J Pharm 356:345-50. 2008
    ..lipid concentration range for lyophilization of sterically stabilized phospholipid nanomicelles (SSM) and the freeze drying feasibility of self-associated therapeutic peptide-SSM assemblies...
  14. ncbi Effect of pH value of freeze-drying solution on the chromosome integrity and developmental ability of mouse spermatozoa
    Takehito Kaneko
    The Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 68:136-9. 2003
    ..0) rather than near neutral or acidic (pH 7.4-6.0). The data indicated that the chromosome integrity and developmental ability of mouse spermatozoa are affected by the pH value of freeze-drying solution...
  15. ncbi Preparation of DNA dry powder for non-viral gene delivery by spray-freeze drying: effect of protective agents (polyethyleneimine and sugars) on the stability of DNA
    Jung Hua Steven Kuo
    Department of Biotechnology, Chia Nan University of Pharmacy and Science, 60 Erh Jen Rd, Sec 1, Jen Te, Tainan 717, Taiwan, ROC
    J Pharm Pharmacol 56:27-33. 2004
    This study investigates the feasibility of using the process of spray-freeze drying (SFD) to produce DNA dry powders for non-viral gene delivery. The effect of protective agents was assessed on the stability of DNA dry powders after SFD...
  16. ncbi Luminescent bacterial sensor for cadmium and lead
    S Tauriainen
    University of Turku, Department of Biotechnology, Finland
    Biosens Bioelectron 13:931-8. 1998
    ..These results were obtained with only 2-3 h incubation times. Freeze-drying of the sensor strains had only moderate effects on the performance with respect to sensitivity or induction coefficients...
  17. ncbi Localized viral vector delivery to enhance in situ regenerative gene therapy
    W W Hu
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 1078, USA
    Gene Ther 14:891-901. 2007
    ..In contrast, AdBMP-2 lyophilized in gelatin sponges led to more than 80% regeneration of critical-size calvarial defects...
  18. ncbi Long-term preservation of mouse spermatozoa after freeze-drying and freezing without cryoprotection
    Monika A Ward
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
    Biol Reprod 69:2100-8. 2003
    ..Both preservation methods are successful, but rapid freezing without cryoprotection is the preferred method for preservation of spermatozoa from mouse strains carrying unique genes and mutations...
  19. ncbi Freeze-drying of nanoparticles: formulation, process and storage considerations
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés LAGEP UMR CNRS 5007 CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 43, Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Adv Drug Deliv Rev 58:1688-713. 2006
    ....
  20. ncbi Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells
    Tetsuo Ono
    Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe, Japan
    J Reprod Dev 54:486-91. 2008
    ..Our findings show that the genomic integrity of cells can be maintained after freeze-drying and that it is possible to produce offspring from the cells using nuclear transfer techniques...
  21. doi Impact of matrix properties on the survival of freeze-dried bacteria
    Per Wessman
    Department of Microbiology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7025, SE 750 07 Uppsala, Sweden
    J Sci Food Agric 91:2518-28. 2011
    ..hydroxypropylmethylcellulose and polyvinylalcohol to support the survival of three bacterial strains during freeze drying. The initial osmotic conditions were adjusted to be similar for all formulations...
  22. doi Effect of culture age, protectants, and initial cell concentration on viability of freeze-dried cells of Metschnikowia pulcherrima
    Davide Spadaro
    Department of Exploitation and Protection of Agricultural and Forestry Resources, University of Turin, Grugliasco, TO, Italy
    Can J Microbiol 56:809-15. 2010
    ..pulcherrima cells was similar to that obtained in other studies. The results constitute a first step towards the commercial development of M. pulcherrima as a biocontrol agent...
  23. ncbi Factors affecting short-term and long-term stabilities of proteins
    T Arakawa
    Amgen Inc, Amgen Center, Thousand Oaks, CA, USA
    Adv Drug Deliv Rev 46:307-26. 2001
    ..This review discusses the current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses, and also the various factors to be considered for long-term storage of proteins in solution...
  24. ncbi Effect of intracellular trehalose in Cryptococcus laurentii and exogenous lyoprotectants on its viability and biocontrol efficacy on Penicillium expansum in apple fruit
    B Q Li
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
    Lett Appl Microbiol 44:437-42. 2007
    To improve viability and biocontrol efficacy of Cryptococcus laurentii after freeze drying and in subsequent storage.
  25. ncbi First steps towards tissue engineering of small-diameter blood vessels: preparation of flat scaffolds of collagen and elastin by means of freeze drying
    L Buttafoco
    Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology and Institute of Biomedical Technology BMTI, University of Twente, Enschede, 7500AE, The Netherlands
    J Biomed Mater Res B Appl Biomater 77:357-68. 2006
    Porous scaffolds composed of collagen or collagen and elastin were prepared by freeze drying at temperatures between -18 and -196 degrees C. All scaffolds had a porosity of 90-98% and a homogeneous distribution of pores...
  26. doi Survival of freeze-dried leuconostoc mesenteroides and Lactobacillus plantarum related to their cellular fatty acids composition during storage
    Ibourahema Coulibaly
    Unité des Bio industries, Centre Wallon de Biologie Industrielle, Faculte Universitaire des Sciences Agronomiques de Gembloux, Passage des Deportes, Gembloux, Belgium
    Appl Biochem Biotechnol 157:70-84. 2009
    ..mesenteroides and L. plantarum was noted in the freeze-dried powder held at 4 degrees C or under vacuum and in dark than at 20 degrees C or in the presence of oxygen and light...
  27. ncbi Freeze-drying fungi using a shelf freeze-drier
    C Shu hui Tan
    Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands
    Methods Mol Biol 368:119-25. 2007
    ..This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture collection (http://www.cbs.knaw.nl/)...
  28. ncbi Viability, efficacy, and storage stability of freeze-dried biocontrol agent Candida sake using different protective and rehydration media
    M Abadias
    Postharvest Unit, CeRTA, Centre UdL IRTA, Lleida, Catalonia, Spain
    J Food Prot 64:856-61. 2001
    ..The effect of several protective agents and rehydration media was investigated in the freeze drying of C. sake. Skimmed milk at 10% concentration was a good rehydration medium for all protectants tested...
  29. pmc Lyophilization prior to direct DNA extraction from bovine feces improves the quantification of Escherichia coli O157:H7 and Campylobacter jejuni
    Delphine Rapp
    Climate, Land and Environment, AgResearch, East Street, Private Bag 3123, Hamilton 3240, New Zealand
    Appl Environ Microbiol 76:1686-8. 2010
    ....
  30. ncbi Molecular cloning of mRNA from toad granular gland secretion and lyophilized skin: identification of Bo8--a novel prokineticin from Bombina orientalis
    Tianbao Chen
    Molecular Therapeutics Research Group, School of Pharmacy, Queen s University, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK
    Peptides 26:377-83. 2005
    ..Amino acid substitutions in skin prokineticins from the skin secretions of bombinid toads are confined to discrete sites affording the necessary information for structure/activity studies and analog design...
  31. ncbi Survival curves for microbial species stored by freeze-drying
    Yukie Miyamoto-Shinohara
    International Patent Organism Depository, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Cryobiology 52:27-32. 2006
    ..The excellent survival after freeze-drying of each species might be attributable to the high level of desiccation and to sealing under vacuum...
  32. ncbi Unmasking venom gland transcriptomes in reptile venoms
    Tianbao Chen
    Pharmaceutical Biotechnology, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Ireland
    Anal Biochem 311:152-6. 2002
    ....
  33. ncbi Freeze-dried sperm fertilization leads to full-term development in rabbits
    Ji Long Liu
    Department of Animal Science Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Biol Reprod 70:1776-81. 2004
    ..The present study also raises the question of whether rabbit sperm centrosomes survive freeze-drying or are not essential for embryonic development...
  34. ncbi Tolerance of the mouse sperm nuclei to freeze-drying depends on their disulfide status
    Takehito Kaneko
    Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 69:1859-62. 2003
    ..The potential for freeze-drying damage seems likely to relate to the -SS- status of sperm proteins, in particular its protamines...
  35. ncbi Glycine betaine as a cryoprotectant for prokaryotes
    David Cleland
    Bacteriology Program, American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110, USA
    J Microbiol Methods 58:31-8. 2004
    ..These results indicate that GB is a simple and useful cryoprotectant that works for a wide range of prokaryotic organisms under different cryopreservation regimens...
  36. ncbi Offspring derived from oocytes injected with rat sperm, frozen or freeze-dried without cryoprotection
    T Kaneko
    Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, 2 2 1 Honjo, Kumamoto, Japan
    Theriogenology 68:1017-21. 2007
    ..We concluded that this simple preservation method, in which cryoprotection was not required, allowed sperm to be preserved efficiently with maintenance of their fertilizing ability...
  37. ncbi Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa
    In Kiu Kwon
    The Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Biol Reprod 71:1430-6. 2004
    ..These results demonstrate that pig oocytes can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst stage...
  38. ncbi Preservation of micro-organisms by drying; a review
    C A Morgan
    BTF Pty Ltd, PO Box 599, North Ryde BC, NSW 1670, Australia
    J Microbiol Methods 66:183-93. 2006
    The preservation of micro-organisms by different drying methodologies has been used for decades. Freeze drying in particular is the preferred method for transporting and storing vast culture collections of micro-organism strain types...
  39. ncbi Improvement in the long-term stability of freeze-dried mouse spermatozoa by adding of a chelating agent
    Takehito Kaneko
    Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, Kumamoto 860 0811, Japan
    Cryobiology 53:279-82. 2006
    ..This finding suggested that mouse spermatozoa can be freeze-dried in a simple solution containing the same composition as that used to preserve extracted DNA...
  40. ncbi Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads
    Masumi Hirabayashi
    National Institute for Physiological Sciences, The Graduate University for Advanced Studies, Okazaki, Aichi, Japan
    Zygote 13:79-85. 2005
    ..Thus, the successful participation of freeze-dried spermatozoa in full-term development was demonstrated by applying ICSI in the rat...
  41. ncbi Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa
    Levent Keskintepe
    Transgenic and Embryonic Stem Cell Core, IMMAG, Medical College of Georgia, Augusta, GA 30912, USA
    Biol Reprod 67:409-15. 2002
    ..These results demonstrate that in vitro-matured bovine oocytes can be fertilized with freeze-dried sperm cells, and that resultant zygotes can develop into karyotypically normal blastocysts...
  42. doi Encapsulation and sustained release from biodegradable microcapsules made by emulsification/freeze drying and spray/freeze drying
    Weisi Yin
    Department of Chemical Engineering, University of Rochester, 206 Gavett Hall, Rochester, NY 14627, USA
    J Colloid Interface Sci 336:155-61. 2009
    Hollow biodegradable poly(DL-lactide) (PLA) particles with porous shell walls were prepared by freeze drying small droplets of PLA solution formed by emulsification or spraying...
  43. doi Importance of primary culture conditions for the development of rat ICSI embryos and long-term preservation of freeze-dried sperm
    Takehito Kaneko
    Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, Honjo, Kumamoto, Japan
    Cryobiology 58:293-7. 2009
    ..Also, rat sperm after freeze-drying in TE buffer were preserved at 4 degrees C for long term without their deterioration...
  44. pmc Controlled crystallization of the lipophilic drug fenofibrate during freeze-drying: elucidation of the mechanism by in-line Raman spectroscopy
    Hans de Waard
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, The Netherlands
    AAPS J 12:569-75. 2010
    ..In conclusion, for the model drug fenofibrate, a high freezing rate and a relatively low crystallization temperature resulted in the smallest crystals and therefore the highest dissolution rate...
  45. doi Solid state fluorescence of lyophilized proteins
    Ranjini Ramachander
    Product and Process Development, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    Anal Biochem 376:173-82. 2008
    ..This suggests that solid state, intrinsic protein fluorescence measurements using the Cary-Eclipse holder may be feasible for long-term stability studies and formulation development...
  46. pmc Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation
    Tomasz Pniewski
    Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60 479, Poznan, Poland
    J Appl Genet 52:125-36. 2011
    ..The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B...
  47. doi Constant size, variable density aerosol particles by ultrasonic spray freeze drying
    Suzanne M D'Addio
    Advanced Drug Delivery Group, Faculty of Pharmacy, The University of Sydney, Camperdown, NSW 2006, Australia
    Int J Pharm 427:185-91. 2012
    ..of critical process parameters involved in the production of inhalation aerosol particles by ultrasonic spray freeze drying to enable precise control over particle size and aerodynamic properties...
  48. doi Preparation and pharmacokinetics of docetaxel based on nanostructured lipid carriers
    Xiang Li
    Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang, People s Republic of China
    J Pharm Pharmacol 61:1485-92. 2009
    ..This study describes the preparation and pharmacokinetics of docetaxel based on freeze-dried nanostructured lipid carriers (NLCs)...
  49. ncbi Influence of the stabilizer coating layer on the purification and freeze-drying of poly(D,L-lactic acid) nanoparticles prepared by an emulsion-diffusion technique
    D Quintanar Guerrero
    Interuniversity Centre Geneva Lyon, Archamps, France
    J Microencapsul 15:107-19. 1998
    In this study, the purification by cross-flow filtration (CFF) and freeze drying of poly(D,L-lactic acid) (PLA) nanoparticles prepared by an emulsion-diffusion technique using poly(vinyl alcohol) (PVAL) or poloxamer 188 (P-188) were ..
  50. ncbi Freeze-drying of nanocapsules: impact of annealing on the drying process
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés LAGEP, UMR CNRS 5007, CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Int J Pharm 324:74-82. 2006
    ..The acceleration of sublimation rate seems to depend on the temperature of annealing. The annealing of sucrose solution slows down the secondary drying kinetic whereas no effect is observed in the case of PVP...
  51. ncbi From anhydrobiosis to freeze-drying of eukaryotic cells
    Willem F Wolkers
    Department of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Comp Biochem Physiol A Mol Integr Physiol 131:535-43. 2002
    ..We suggest that this finding will obviate the storage problem with platelets and that the principles established here may be extended to more complex eukaryotic cells...
  52. ncbi Visualization of actin filaments in keratocyte lamellipodia: negative staining compared with freeze-drying
    J V Small
    Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg
    J Struct Biol 113:135-41. 1994
    ..Drying in negative stain appears to stabilise the actin network.(ABSTRACT TRUNCATED AT 250 WORDS)..
  53. doi Preparation and characterization of medium-chain fatty acid liposomes by lyophilization
    Chengmei Liu
    State Key Laboratory of Food Science and Technology, Department of Life Science and Food Engineering, Nanchang University, Nanchang, China
    J Liposome Res 20:183-90. 2010
    ..The mean diameters were 251.1 and 265.3 nm, and the encapsulation efficiencies were 80.5 and 79.2% for freshly prepared and reconstituted liposomes, respectively...
  54. pmc The stabilisation of purified, reconstituted P-glycoprotein by freeze drying with disaccharides
    Adam Heikal
    University of Brighton, School of Pharmacy and Biomolecular Sciences, Cockcroft Building, Lewes Road, Brighton BN2 4GJ, UK
    Cryobiology 58:37-44. 2009
    ..The ABCB1 purification procedure was optimised to allow successful freeze drying by substitution of glycerol with the disaccharides trehalose or maltose...
  55. ncbi Freeze-dried primate sperm retains early reproductive potential after intracytoplasmic sperm injection
    Luis Gabriel Sánchez-Partida
    Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    Fertil Steril 89:742-5. 2008
    ..Oocytes inseminated by intracytoplasmic sperm injection using fresh ejaculated or freeze-dried rhesus macaque sperm showed similar activation, sperm aster assembly, and male-female pronuclear apposition rates...
  56. ncbi Lyoprotected nanosphere formulations for paclitaxel controlled delivery
    T Musumeci
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Catania, V le A Doria, 6 I 95125 Catania, Italy
    J Nanosci Nanotechnol 6:3118-25. 2006
    ..The PLGA NS, in particular, showed a cell growth inhibitory activity comparable to the free drug...
  57. doi Freeze-drying of squalenoylated nucleoside analogue nanoparticles
    L Bildstein
    Universite Paris Sud XI, Faculte de Pharmacie, UMR CNRS 8612, IFR 141, 92296 Chatenay Malabry Cedex, France
    Int J Pharm 381:140-5. 2009
    ..Overall, these results allow for the use of freeze-dried NPs in upcoming preclinical trials of the different squalenoylated compounds developed in our laboratory...
  58. doi Preparation of drug nanocrystals by controlled crystallization: application of a 3-way nozzle to prevent premature crystallization for large scale production
    Hans de Waard
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Groningen, The Netherlands
    Eur J Pharm Sci 38:224-9. 2009
    ..W., 2008. A novel bottom-up process to produce drug nanocrystals: controlled crystallization during freeze drying. J. Control. Release 128, 179-183]...
  59. ncbi Bioactive compounds of grapefruit (Citrus paradisi Cv. Rio Red) respond differently to postharvest irradiation, storage, and freeze drying
    Jairam Vanamala
    Texas A and M University Kingsville Citrus Center, 312 N International Blvd, Weslaco, TX 78596, USA
    J Agric Food Chem 53:3980-5. 2005
    In the present study, the effect of irradiation, storage, and freeze drying on grapefruit bioactive compounds was investigated...
  60. ncbi Effect of preparation method on physical properties of amorphous trehalose
    Rahul Surana
    Department of Pharmaceutics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Pharm Res 21:1167-76. 2004
    ..To determine the effect of preparation method on the physical properties of amorphous trehalose...
  61. ncbi Cell biology: just add water
    Geoff Brumfiel
    Nature 428:14-5. 2004
  62. ncbi Influenza vaccine powder formulation development: spray-freeze-drying and stability evaluation
    Yuh Fun Maa
    PowderJect Vaccines, Incorporated, 8551 Research Way Boulevard, Middleton, Wisconsin 53562, USA
    J Pharm Sci 93:1912-23. 2004
    ..More important, this study led to the selection of a formulation system that had been successfully tested in a human clinical study...
  63. ncbi Inulin sugar glasses preserve the structural integrity and biological activity of influenza virosomes during freeze-drying and storage
    Jørgen de Jonge
    Department of Medical Microbiology, Molecular Virology Section, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands
    Eur J Pharm Sci 32:33-44. 2007
    ..Formulation of influenza virosomes as a dry-powder is advantageous for storage and transport and offers the possibility to develop needle-free dosage forms, e.g. for oral, nasal, pulmonal, or dermal delivery...
  64. ncbi Inulin is a promising cryo- and lyoprotectant for PEGylated lipoplexes
    W L J Hinrichs
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    J Control Release 103:465-79. 2005
    ..Compared to dextran, inulin was also a superior lyoprotectant of PEGylated lipoplexes: during freeze drying and subsequent storage at 37 degrees C of the dried powders for 3 months the PEGylated lipoplexes maintained ..
  65. ncbi Critical freezing rate in freeze drying nanocrystal dispersions
    Jonghwi Lee
    Department of Chemical Engineering and Materials Science, Chung Ang University, 221 Heukseok dong, Dongjak Gu, Seoul 156 756, Korea South
    J Control Release 111:185-92. 2006
    ..Herein, the freeze drying process of nanocrystal dispersions was examined at varying freezing rates (speed of freezing interface)...
  66. ncbi The choice of a suitable oligosaccharide to prevent aggregation of PEGylated nanoparticles during freeze thawing and freeze drying
    W L J Hinrichs
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Int J Pharm 311:237-44. 2006
    ..PEG) coated plasmid DNA/cationic liposome complexes ("PEGylated lipoplexes") during freeze thawing and freeze drying [Hinrichs et al., 2005. J. Control. Release 103, 465]. By contrast, dextran clearly failed as stabilizer...
  67. ncbi Implementation of a process analytical technology system in a freeze-drying process using Raman spectroscopy for in-line process monitoring
    T R M De Beer
    Laboratory of Drug Quality Control, Department of Pharmaceutical Analysis, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Anal Chem 79:7992-8003. 2007
    ..Raman spectroscopy (in-line) and NIR spectroscopy and XRPD (at-line) not only allowed the real-time monitoring of mannitol freeze-drying processes but also helped (in combination with experimental design) us to understand the process...
  68. ncbi Preservation of influenza virosome structure and function during freeze-drying and storage
    Jan Wilschut
    Department of Medical Microbiology, Molecular Virology Section, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    J Liposome Res 17:173-82. 2007
    ..It is concluded that freeze-drying in the presence of inulin as a lyoprotectant completely preserves the structure and function of influenza virosomes...
  69. ncbi Rational design of an influenza subunit vaccine powder with sugar glass technology: preventing conformational changes of haemagglutinin during freezing and freeze-drying
    J P Amorij
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Vaccine 25:6447-57. 2007
    ..Moreover, the HA potency of the influenza subunit vaccine powder was stable for at least 26 weeks at room temperature...
  70. ncbi Monitoring of mannitol phase behavior during freeze-drying using non-invasive Raman spectroscopy
    Saly Romero-Torres
    Department of Chemistry, Purdue University, West Lafayette, IN 47907 2091, USA
    PDA J Pharm Sci Technol 61:131-45. 2007
    ..In conclusion, Raman spectroscopy is a potentially useful technique to monitor physical changes during freeze-drying...
  71. doi A novel bottom-up process to produce drug nanocrystals: controlled crystallization during freeze-drying
    H de Waard
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    J Control Release 128:179-83. 2008
    To improve the dissolution behavior of lipophilic drugs, a novel bottom-up process based upon freeze drying which allows for the production of nanocrystalline particles was developed: "controlled crystallization during freeze drying"...
  72. ncbi Annealing to optimize the primary drying rate, reduce freezing-induced drying rate heterogeneity, and determine T(g)' in pharmaceutical lyophilization
    J A Searles
    Center for Pharmaceutical Biotechnology and Department of Chemical Engineering, University of Colorado, Boulder, Colorado 80309, USA
    J Pharm Sci 90:872-87. 2001
    ..It can be carried out with a balance and a freeze-dryer, and has the additional advantage that a large number of candidate formulations can be evaluated simultaneously...
  73. pmc Development of stable influenza vaccine powder formulations: challenges and possibilities
    J P Amorij
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Groningen, The Netherlands
    Pharm Res 25:1256-73. 2008
    ..e. lyophilization, spray drying, spray-freeze drying, vacuum drying or supercritical fluid drying), the current status of dry influenza vaccine development and the ..
  74. pmc Freeze-dried somatic cells direct embryonic development after nuclear transfer
    Pasqualino Loi
    Department of Comparative Biomedical Sciences, Teramo University, Teramo, Italy
    PLoS ONE 3:e2978. 2008
    ..These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine and for conservation strategies...
  75. ncbi A modified process for preparing cationic polylactide-co-glycolide microparticles with adsorbed DNA
    Manmohan Singh
    Novartis Vaccines, 4560 Horton Street, Emeryville, CA 94608, USA
    Int J Pharm 327:1-5. 2006
    ..The modified process with a single lyophilization step is a more practical process and can be utlized to prepare cationic PLG microparticles with adsorbed DNA on a large scale...
  76. ncbi Impact of freezing procedure and annealing on the physico-chemical properties and the formation of mannitol hydrate in mannitol-sucrose-NaCl formulations
    Andrea Hawe
    Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig Maximilians University Munich, Munich, Germany
    Eur J Pharm Biopharm 64:316-25. 2006
    ..For the development of lyophilization cycles the lowered Tg', as well as the tendency to mannitol hydrate formation predominantly at lower temperature needs to be considered...
  77. doi Preparation of preformed porous PLGA microparticles and antisense oligonucleotides loading
    Abid Riaz Ahmed
    College of Pharmacy, Freie Universitat Berlin, Berlin, Germany
    Eur J Pharm Biopharm 71:264-70. 2009
    ..The initial burst was dependent on the drug loading and could be significantly reduced by wet (non-aqueous) temperature curing...
  78. doi Short- and long-term stability study of lyophilized solid lipid nanoparticles for gene therapy
    A del Pozo-Rodríguez
    Pharmacy and Pharmaceutical Technology Laboratory, University of the Basque Country UPV EHU, Vitoria Gasteiz, Spain
    Eur J Pharm Biopharm 71:181-9. 2009
    ..Furthermore, the storage of lyophilized lipoplexes stabilized with the disaccharide trehalose did not affect cell viability...
  79. pmc Formulation of a dry powder influenza vaccine for nasal delivery
    Robert J Garmise
    School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    AAPS PharmSciTech 7:E19. 2006
    ..Further optimization of particle size and morphology is required for these powders to be suitable for clinical evaluation...
  80. doi The relation between moisture-induced aggregation and structural changes in lyophilized insulin
    Giselle M Flores-Fernández
    Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, San Juan, Puerto Rico
    J Pharm Pharmacol 61:1555-61. 2009
    ..We have investigated the structural consequences of moisture sorption by lyophilized insulin under controlled humidity conditions by employing Fourier transform-infrared (FT-IR) microscopy...
  81. ncbi Pulmonary delivery of an inulin-stabilized influenza subunit vaccine prepared by spray-freeze drying induces systemic, mucosal humoral as well as cell-mediated immune responses in BALB/c mice
    J P Amorij
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Vaccine 25:8707-17. 2007
    ..Vaccine powder was produced by spray-freeze drying (SFD) using the oligosaccharide inulin as stabilizer...
  82. ncbi Acetabular revision with impacted freeze-dried cancellous bone chips and a cemented cup: a report of 7 cases at 5 to 9 years' follow-up
    T M Thien
    Department of Orthopaedics, University Medical Centre Nijmegen, Postbox 9101, 6500 HB Nijmegen, The Netherlands
    J Arthroplasty 16:666-70. 2001
    ..At midterm follow-up, there was no aseptic loosening. In this limited case report, the results at midterm for freeze-dried allograft bone chips in acetabular reconstructions are acceptable...
  83. ncbi Inulin glasses for the stabilization of therapeutic proteins
    W L Hinrichs
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands
    Int J Pharm 215:163-74. 2001
    ..When freeze drying is envisaged as method of drying, a relatively high Tg of the freeze concentrated fraction (Tg') is ..
  84. ncbi Impaction bone grafting using freeze-dried allograft in revision hip arthroplasty
    N J de Roeck
    Orthopaedic Research Unit, East Surrey Hospital, Redhill, Surrey RH1 5RH, United Kingdom
    J Arthroplasty 16:201-6. 2001
    ..The results of impaction bone grafting with freeze-dried bone alone have been satisfactory, although we recommend caution in its use alone in the hostile acetabulum...
  85. ncbi Protein spray-freeze drying. Effect of atomization conditions on particle size and stability
    H R Costantino
    Alkermes, Inc, Cambridge, MA 02139, USA
    Pharm Res 17:1374-83. 2000
    ..To investigate the effect of atomization conditions on particle size and stability of spray-freeze dried protein...
  86. ncbi Protein spray freeze drying. 2. Effect of formulation variables on particle size and stability
    Henry R Costantino
    Alkermes, Inc, 64 Sidney Street, Cambridge, Massachusetts 02139, USA
    J Pharm Sci 91:388-95. 2002
    Spray freeze drying produces protein particles suitable for microencapsulation into polymeric microspheres intended for sustained release...
  87. ncbi Lyophilization and development of solid protein pharmaceuticals
    W Wang
    Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 203:1-60. 2000
    ....
  88. ncbi Factors that influence stability of recombinant adenoviral preparations for human gene therapy
    M A Croyle
    College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    Pharm Dev Technol 3:373-83. 1998
    ..Both formulations provided adequate stability for the adenovirus, with 2.6 and 5.6 x 10(11) lfu/ml detected 150 days after drying, respectively...
  89. ncbi Thrombus formation with rehydrated, lyophilized platelets
    Thomas H Fischer
    Department of Pathology, 350 Old Fayetteville Road, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
    Hematology 7:359-69. 2002
    ..Our results help explain how RL platelets restore hemostasis in vivo, and indicate that these cells might be a viable alternative to fresh stored platelets in transfusion medicine...
  90. ncbi Investigations into the stabilization of drugs by sugar glasses: III. The influence of various high-pH buffers
    Jonas H C Eriksson
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, 9713 AV Groningen, The Netherlands
    Pharm Res 20:1437-43. 2003
    ..0 and pH 9.8. Also, the glass transition temperature (Tg) of sugar glasses obtained after freeze drying of these solutions was elucidated...
  91. ncbi Specific effects of fructo- and gluco-oligosaccharides in the preservation of liposomes during drying
    Dirk K Hincha
    Max Planck Institut fur Molekulare Pflanzenphysiologie, D 14424 Potsdam, Germany
    Glycobiology 12:103-10. 2002
    ..Collectively, our data indicate that the fructo-oligosaccharides accumulated in many plant species under stress conditions could indeed play an important role in cellular dehydration tolerance...
  92. ncbi Spray-drying of trypsin - surface characterisation and activity preservation
    A Millqvist-Fureby
    Institute for Surface Chemistry, P O Box 5607, SE 114 86, Stockholm, Sweden
    Int J Pharm 188:243-53. 1999
    ..For mannitol and sucrose, however, the level of inactivation was higher than could be explained by surface inactivation alone, and additional mechanisms must also be considered...
  93. ncbi Impaction bone grafting with freeze-dried irradiated bone. Part I. Femoral implant stability: cadaver experiments in a hip simulator
    Olivier Cornu
    Orthopaedic Research Laboratory, Universite Catholique de Louvain, Brussels, Belgium
    Acta Orthop Scand 74:547-52. 2003
    ..Freeze-dried grafts provide more stable fixation of the stem than fresh-frozen morselized grafts, when tested in a hip simulator...
  94. ncbi Relationship between conformational stability and lyophilization-induced structural changes in chymotrypsin
    K G Carrasquillo
    Department of Chemistry, University of Puerto Rico, , P.O. Box 23346, San Juan, PR 00931-3346, Puerto Rico
    Biotechnol Appl Biochem 31:41-53. 2000
    ..This can be understood because the dehydration and not the freezing process, as shown in an air-drying experiment, mainly causes protein structural alterations...
  95. ncbi Instability, stabilization, and formulation of liquid protein pharmaceuticals
    W Wang
    Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 185:129-88. 1999
    ....
  96. ncbi Protein inhalation powders: spray drying vs spray freeze drying
    Y F Maa
    Genentech, Inc, South San Francisco, California 94080, USA
    Pharm Res 16:249-54. 1999
    To develop a new technique, spray freeze drying, for preparing protein aerosol powders. Also, to compare the spray freeze-dried powders with spray-dried powders in terms of physical properties and aerosol performance.
  97. ncbi A solid-state NMR study of protein hydration and stability
    F Separovic
    School of Chemistry, University of Melbourne, Parkville, Vic, Australia
    Pharm Res 15:1816-21. 1998
    ..The mobility of protein in powders at different hydration levels was studied in relation to aggregation and activity...
  98. pmc Stability of dry liposomes in sugar glasses
    W Q Sun
    Department of Botany, National University of Singapore, Singapore
    Biophys J 70:1769-76. 1996
    ..The half-life of solute retention in dry liposomes may be prolonged by storing dry liposomes at temperatures below the Tg and by increasing the Tg of the dry liposome preparation...
  99. ncbi A small stress protein acts synergistically with trehalose to confer desiccation tolerance on mammalian cells
    Xiaocui Ma
    UC Davis Medical Center, Department of Internal Medicine, Transplant Research Program, 4635 2nd Avenue, Suite 1001, Sacramento, CA 95817, USA
    Cryobiology 51:15-28. 2005
    ..2 g H2O/g dry weight, as measured by gravimetric analysis. These results indicate that p26 improves cell survival following drying and rehydration, and suggest that dry storage of mammalian cells is a likely possibility in the future...
  100. ncbi The stability of lyophilized lipid/DNA complexes during prolonged storage
    Marion d C Molina
    Center for Pharmaceutical Biotechnology, University of Colorado School of Pharmacy, Denver, Colorado 80262, USA
    J Pharm Sci 93:2259-73. 2004
    ....
  101. ncbi A novel method for the preparation of liposomes: freeze drying of monophase solutions
    Chunlei Li
    Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, No 103, Wenhua Road, P O Box 52, Shenyang City, Liaoning Province, 110016, People s Republic of China
    J Pharm Sci 93:1403-14. 2004
    ..In addition, the application of this new method to the passive loading and active loading of drugs into liposomes is discussed in detail...

Research Grants70

  1. A Thermostable Measles Vaccine
    Jeffrey Griffiths; Fiscal Year: 2009
    ..Vaccine campaigns have failed to eliminate measles because the vaccine goes bad when it is heated. Our project is to make a new vaccine that will not lose its ability to protect people if there is no refrigeration. ..
  2. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
    ..are treated with various chemicals (detergents, DTT, exogenous DNA) or are subjected to mechanical insults (freeze drying and freezing without cryoprotection), and then injected into the oocytes, the paternal chromosomes in the ..
  3. Freeze-Dried, Viable Allogeneic Heart Valves
    Kelvin Brockbank; Fiscal Year: 2006
    ..Phase II studies will be performed to develop formulations and methods that permit storage at refrigeration or ambient temperatures and scale-up the resulting freeze-drying technology to sheep and human heart valves. ..
  4. Stable Micronized Vaccines Against Smallpox and Japanese Encephalitis
    VICTOR BRONSHTEIN; Fiscal Year: 2007
    ....
  5. Novel Osteoinductive Protein Synthesizing Implant System
    Kyumin Whang; Fiscal Year: 2003
    ....
  6. Revitalizing Allografts for Craniofacial Reconstruction
    Edward Schwarz; Fiscal Year: 2007
    ....
  7. A Thermostable Measles Vaccine
    Jeffrey K Griffiths; Fiscal Year: 2010
    ..Vaccine campaigns have failed to eliminate measles because the vaccine goes bad when it is heated. Our project is to make a new vaccine that will not lose its ability to protect people if there is no refrigeration. ..
  8. Fluorescence Sensor Probe for Transcutaneous Glucose Sensing
    Ralph Ballerstadt; Fiscal Year: 2007
    ..The overall result will be a healthier life for people with diabetes and lower medical costs for diabetes care for the society. ..
  9. DIGESTION OF FOOD OXALATE
    Harmeet Sidhu; Fiscal Year: 2002
    ..The third specific aim will optimize fermentation and freeze drying conditions to produce a product with satisfactory cost, efficacy, safety and shelf life...
  10. LYOPHILIZATION OF NONVIRAL GENE DELIVERY SYSTEMS
    THOMAS ANCHORDOQUY; Fiscal Year: 2002
    ..In addition to designing excipient formulations to stabilize lyophilized preparations, these studies will help to better characterize the "native state" of synthetic gene delivery systems. ..
  11. Biochemical Stabilization for Freeze Dried Platelets
    Christopher Wagner; Fiscal Year: 2003
    ..abstract_text> ..
  12. Breaking Tolerance to a Tumor associated Antigen
    Scott Bingham; Fiscal Year: 2005
    ..We believe the novel delivery approach of this vaccine has potential to provide a broad immune response more capable of decreasing if not preventing tumor burden. ..
  13. NOVEL APPROACHES TO IMPROVE AND EXTEND PLATELET STORAGE
    Sherrill Slichter; Fiscal Year: 2001
    ....
  14. Stabilized Platelets for Advanced Wound Healing
    Cindy Orser; Fiscal Year: 2004
    ..loading methodologies, from endocytotic to peptide-mediated, will be evaluated for their applicability for pre-freeze drying loading...
  15. CONCENTRATION METHOD FOR THERMALLY LABILE PHARMACEUTICAL
    JOHN BOWSER; Fiscal Year: 2004
    ....
  16. Formulation of Synthetic Vectors as Lyophilized Products
    THOMAS ANCHORDOQUY; Fiscal Year: 2007
    ..g., lipoplexes and polyplexes) in order to develop rational formulation guidelines that are generally applicable to the stabilization of macromolecular complexes (e.g., vaccines, viruses, nanoparticles) during freeze-drying and storage. ..
  17. Formulation of Synthetic Vectors as Lyophilized Products
    THOMAS ANCHORDOQUY; Fiscal Year: 2009
    ..g., vaccines, viruses, nanoparticles) during freeze-drying and storage. ..
  18. ORIGIN OF CELLS THAT REPOPULATE RESORBABLE ECM SCAFFOLDS
    Stephen Badylak; Fiscal Year: 2003
    ..abstract_text> ..
  19. Cell Recruitment Induced by ECM Scaffold Degradation
    Stephen Badylak; Fiscal Year: 2009
    ..The objectives of the studies, the hypothesis upon which the experimental design is based, and a timeline for completion of the studies are presented. ..