freeze drying

Summary

Summary: Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.

Top Publications

  1. ncbi Spray freeze drying to produce a stable Delta(9)-tetrahydrocannabinol containing inulin-based solid dispersion powder suitable for inhalation
    Dirk Jan van Drooge
    Department of Pharmaceutical Technology and Biopharmacy, Groningen University Institute for Drug Exploration GUIDE, Antonius Deusinglaan 1, 9713AV Groningen, The Netherlands
    Eur J Pharm Sci 26:231-40. 2005
  2. ncbi Stabilization of bacteriophage during freeze drying
    U Puapermpoonsiri
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Strathclyde G4 0NR, UK
    Int J Pharm 389:168-75. 2010
  3. ncbi Freeze-drying of nanoparticles: formulation, process and storage considerations
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés LAGEP UMR CNRS 5007 CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 43, Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Adv Drug Deliv Rev 58:1688-713. 2006
  4. ncbi Effect of culture medium and cryoprotectants on the growth and survival of probiotic lactobacilli during freeze drying
    A Siaterlis
    Department of Food Biosciences, The University of Reading, Berkshire, Reading, UK
    Lett Appl Microbiol 48:295-301. 2009
  5. ncbi Lyophilization and development of solid protein pharmaceuticals
    W Wang
    Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 203:1-60. 2000
  6. ncbi Technological and biological evaluation of tablets containing different strains of lactobacilli for vaginal administration
    L Maggi
    Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy
    Eur J Pharm Biopharm 50:389-95. 2000
  7. ncbi Characterisation of freeze-dried wafers and solvent evaporated films as potential drug delivery systems to mucosal surfaces
    Joshua S Boateng
    Department of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, UK
    Int J Pharm 389:24-31. 2010
  8. ncbi Gelatin coated hybrid lipid nanoparticles for oral delivery of amphotericin B
    Sanyog Jain
    Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar Mohali, Punjab 160062, India
    Mol Pharm 9:2542-53. 2012
  9. ncbi The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state
    J H Crowe
    Biostabilization Program, University of California, Davis, CA 95616, USA
    Cryobiology 43:89-105. 2001
  10. pmc Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation
    Tomasz Pniewski
    Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60 479, Poznan, Poland
    J Appl Genet 52:125-36. 2011

Detail Information

Publications351 found, 100 shown here

  1. ncbi Spray freeze drying to produce a stable Delta(9)-tetrahydrocannabinol containing inulin-based solid dispersion powder suitable for inhalation
    Dirk Jan van Drooge
    Department of Pharmaceutical Technology and Biopharmacy, Groningen University Institute for Drug Exploration GUIDE, Antonius Deusinglaan 1, 9713AV Groningen, The Netherlands
    Eur J Pharm Sci 26:231-40. 2005
    The purpose of this study is to investigate whether spray freeze drying produces an inhalable solid dispersion powder in which Delta(9)-tetrahydrocannabinol (THC) is stabilised...
  2. ncbi Stabilization of bacteriophage during freeze drying
    U Puapermpoonsiri
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor St, Glasgow, Strathclyde G4 0NR, UK
    Int J Pharm 389:168-75. 2010
    ..We propose that residual moisture is followed during lyophilization of bacteriophage from minimal concentrations of bulking agent...
  3. ncbi Freeze-drying of nanoparticles: formulation, process and storage considerations
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés LAGEP UMR CNRS 5007 CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 43, Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Adv Drug Deliv Rev 58:1688-713. 2006
    ....
  4. ncbi Effect of culture medium and cryoprotectants on the growth and survival of probiotic lactobacilli during freeze drying
    A Siaterlis
    Department of Food Biosciences, The University of Reading, Berkshire, Reading, UK
    Lett Appl Microbiol 48:295-301. 2009
    To investigate the effects of the medium and cryoprotective agents used on the growth and survival of Lactobacillus plantarum and Lactobacillus rhamnosus GG during freeze drying.
  5. ncbi Lyophilization and development of solid protein pharmaceuticals
    W Wang
    Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 203:1-60. 2000
    ....
  6. ncbi Technological and biological evaluation of tablets containing different strains of lactobacilli for vaginal administration
    L Maggi
    Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy
    Eur J Pharm Biopharm 50:389-95. 2000
    ..A larger batch of tablets made with a mixture of the three strains was then evaluated, confirming the feasibility of their industrial production and a good bacterial viability in the final dosage form...
  7. ncbi Characterisation of freeze-dried wafers and solvent evaporated films as potential drug delivery systems to mucosal surfaces
    Joshua S Boateng
    Department of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, UK
    Int J Pharm 389:24-31. 2010
    ....
  8. ncbi Gelatin coated hybrid lipid nanoparticles for oral delivery of amphotericin B
    Sanyog Jain
    Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Sector 67, SAS Nagar Mohali, Punjab 160062, India
    Mol Pharm 9:2542-53. 2012
    ..The histopathology of the kidney tissues further confirmed the absence of any changes in the morphology of the renal tubules...
  9. ncbi The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state
    J H Crowe
    Biostabilization Program, University of California, Davis, CA 95616, USA
    Cryobiology 43:89-105. 2001
    ..We review the available mechanisms for introducing trehalose into the cytoplasm of living cells as an introduction to the papers that follow...
  10. pmc Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation
    Tomasz Pniewski
    Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60 479, Poznan, Poland
    J Appl Genet 52:125-36. 2011
    ..The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B...
  11. ncbi Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year
    Shinichi Hochi
    Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan
    Mol Reprod Dev 75:890-4. 2008
    ..In conclusion, rat spermatozoa freeze-dried and stored at +4 degrees C for 1 year are capable of participating in full-term development after ICSI...
  12. ncbi Lyophilized inserts for nasal administration harboring bacteriophage selective for Staphylococcus aureus: in vitro evaluation
    Munerah Alfadhel
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UK
    Int J Pharm 416:280-7. 2011
    ..Our results therefore suggest that lyophilized inserts harboring bacteriophage selective for S. aureus may be a novel means for the eradication of MRSA resident in the nose...
  13. ncbi Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa
    Levent Keskintepe
    Transgenic and Embryonic Stem Cell Core, IMMAG, Medical College of Georgia, Augusta, GA 30912, USA
    Biol Reprod 67:409-15. 2002
    ..These results demonstrate that in vitro-matured bovine oocytes can be fertilized with freeze-dried sperm cells, and that resultant zygotes can develop into karyotypically normal blastocysts...
  14. ncbi Lyophilization monophase solution technique for improvement of the physicochemical properties of an anticancer drug, flutamide
    Nazik Elgindy
    Department of Industrial Pharmacy, University of Alexandria, Alexandria, Egypt
    Eur J Pharm Biopharm 74:397-405. 2010
    ..These data suggest that cyclodextrins might be useful adjuncts in preparation of immediate-release formulations of FLT...
  15. ncbi Preservation of micro-organisms by drying; a review
    C A Morgan
    BTF Pty Ltd, PO Box 599, North Ryde BC, NSW 1670, Australia
    J Microbiol Methods 66:183-93. 2006
    The preservation of micro-organisms by different drying methodologies has been used for decades. Freeze drying in particular is the preferred method for transporting and storing vast culture collections of micro-organism strain types...
  16. ncbi Freeze-dried sperm fertilization leads to full-term development in rabbits
    Ji Long Liu
    Department of Animal Science Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Biol Reprod 70:1776-81. 2004
    ..The present study also raises the question of whether rabbit sperm centrosomes survive freeze-drying or are not essential for embryonic development...
  17. ncbi An overview of liposome lyophilization and its future potential
    Chengjun Chen
    College of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road, No 103, Shenyang, 110016, PR China
    J Control Release 142:299-311. 2010
    ..Finally, the major areas and future potential of research on lyophilized liposomes are highlighted...
  18. ncbi Desiccation tolerance of spermatozoa dried at ambient temperature: production of fetal mice
    Sankha Bhowmick
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02115, USA
    Biol Reprod 68:1779-86. 2003
    ..05). The results demonstrate the feasibility of using a convective drying protocol for the successful desiccation of mouse sperm and identifies some of the important parameters required for optimization of the procedure...
  19. ncbi Structural effects caused by spray- and freeze-drying of liposomes and bilayer disks
    Per Wessman
    Department of Physical and Analytical Chemistry, Uppsala University, Box 579, Uppsala 751 23, Sweden
    J Pharm Sci 99:2032-48. 2010
    ..The radius of bilayer disks increased upon both spray- and freeze-drying, but the drying procedure did not change the open single-bilayer structure of the disks...
  20. pmc Is trehalose special for preserving dry biomaterials?
    L M Crowe
    Section of Molecular and Cellular Biology, University of California, Davis 95616, USA
    Biophys J 71:2087-93. 1996
    ..Because samples remain in the glassy state at ambient temperatures, viscosity is high and fusion between liposomes is prevented...
  21. ncbi Measles virus hemagglutinin protein expressed in transgenic lettuce induces neutralising antibodies in mice following mucosal vaccination
    D E Webster
    The Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne, and School of Biological Sciences, Department of Immunology, Monash University, Vic, Australia
    Vaccine 24:3538-44. 2006
    ..This research represents a significant step towards the development of measles vaccine formulation that is effective, temperature-stable, easy to administer in a resource-poor setting and amenable to large scale manufacture...
  22. ncbi Development of a lyophilized plasmid/LPEI polyplex formulation with long-term stability--A step closer from promising technology to application
    Julia Christina Kasper
    Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig Maximilians University, Butenandtstrasse 5, 81377 Munich, Germany
    J Control Release 151:246-55. 2011
    ..All samples were amorphous with low residual moisture levels (<1.3%) and high glass transition temperatures (>90°C)...
  23. ncbi In vitro drug release studies of polymeric freeze-dried wafers and solvent-cast films using paracetamol as a model soluble drug
    Joshua S Boateng
    Department of Pharmaceutical Sciences, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, UK
    Int J Pharm 378:66-72. 2009
    ..The wafers which formed a porous network, hydrated faster than the more dense and continuous, (non-porous) sheet-like structure of the films...
  24. ncbi A pilot study of freeze drying of poly(epsilon-caprolactone) nanocapsules stabilized by poly(vinyl alcohol): formulation and process optimization
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés UMR CNRS 5007, CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France
    Int J Pharm 309:178-88. 2006
    ..Therefore, freeze drying of these colloidal systems is an alternative method to achieve long-term stability...
  25. ncbi Application of different methods to formulate PEG-liposomes of oxaliplatin: evaluation in vitro and in vivo
    Sara Zalba
    Department of Pharmacy and Pharmaceutical Technology, University of Navarra, Pamplona, Spain
    Eur J Pharm Biopharm 81:273-80. 2012
    ..In conclusion, the FM was the simplest method in comparison with REV and HM to develop in vivo stable and efficient PEG-coated liposomes of oxaliplatin with a loading higher than those reported for REV...
  26. ncbi Effect of pH value of freeze-drying solution on the chromosome integrity and developmental ability of mouse spermatozoa
    Takehito Kaneko
    The Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 68:136-9. 2003
    ..0) rather than near neutral or acidic (pH 7.4-6.0). The data indicated that the chromosome integrity and developmental ability of mouse spermatozoa are affected by the pH value of freeze-drying solution...
  27. pmc Freeze drying of peptide drugs self-associated with long-circulating, biocompatible and biodegradable sterically stabilized phospholipid nanomicelles
    Sok Bee Lim
    Department of Biopharmaceutical Sciences, University of Illinois at Chicago, Chicago, IL 60612 7231, USA
    Int J Pharm 356:345-50. 2008
    ..lipid concentration range for lyophilization of sterically stabilized phospholipid nanomicelles (SSM) and the freeze drying feasibility of self-associated therapeutic peptide-SSM assemblies...
  28. ncbi Freeze-drying of bioproducts: putting principles into practice
    F Franks
    BioUpdate Foundation, Cambridge, UK
    Eur J Pharm Biopharm 45:221-9. 1998
    ....
  29. ncbi Mechanism of protein stabilization by sugars during freeze-drying and storage: native structure preservation, specific interaction, and/or immobilization in a glassy matrix?
    Liuquan Lucy Chang
    Department of Pharmaceutical Science, School of Pharmacy, University of Connecticut, Storrs, CN 06269, USA
    J Pharm Sci 94:1427-44. 2005
    ..However, while a specific interaction between stabilizer and protein might be responsible for the preservation of native structure, the evidence supporting this position is not compelling...
  30. ncbi Preparation of DNA dry powder for non-viral gene delivery by spray-freeze drying: effect of protective agents (polyethyleneimine and sugars) on the stability of DNA
    Jung Hua Steven Kuo
    Department of Biotechnology, Chia Nan University of Pharmacy and Science, 60 Erh Jen Rd, Sec 1, Jen Te, Tainan 717, Taiwan, ROC
    J Pharm Pharmacol 56:27-33. 2004
    This study investigates the feasibility of using the process of spray-freeze drying (SFD) to produce DNA dry powders for non-viral gene delivery. The effect of protective agents was assessed on the stability of DNA dry powders after SFD...
  31. ncbi Luminescent bacterial sensor for cadmium and lead
    S Tauriainen
    University of Turku, Department of Biotechnology, Finland
    Biosens Bioelectron 13:931-8. 1998
    ..These results were obtained with only 2-3 h incubation times. Freeze-drying of the sensor strains had only moderate effects on the performance with respect to sensitivity or induction coefficients...
  32. ncbi In vitro lung delivery of bacteriophages KS4-M and ΦKZ using dry powder inhalers for treatment of Burkholderia cepacia complex and Pseudomonas aeruginosa infections in cystic fibrosis
    L Golshahi
    University of Alberta, Edmonton, AB, Canada
    J Appl Microbiol 110:106-17. 2011
    ..To determine the feasibility of formulating and aerosolizing powders containing bacteriophages KS4-M and ΦKZ for lung delivery and treatment of pulmonary Burkholderia cepacia complex and Pseudomonas aeruginosa infections...
  33. ncbi Localized viral vector delivery to enhance in situ regenerative gene therapy
    W W Hu
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 1078, USA
    Gene Ther 14:891-901. 2007
    ..In contrast, AdBMP-2 lyophilized in gelatin sponges led to more than 80% regeneration of critical-size calvarial defects...
  34. ncbi Effects of the acyl chain composition of phosphatidylcholines on the stability of freeze-dried small liposomes in the presence of maltose
    H Komatsu
    Division of Drugs, National Institute of Health Sciences, Osaka, 540 0005, Japan
    Chem Phys Lipids 113:29-39. 2001
    ..These interactions were significant in the saturated or mixed chain liposomes but were markedly reduced in the DOPC liposomes...
  35. ncbi Practical aspects of lyophilization using non-aqueous co-solvent systems
    Dirk L Teagarden
    Department of Sterile Products Development, Pharmacia Corporation, 7000 Portage Road, 49001 0199, Kalamazoo, MI 49001 0199, USA
    Eur J Pharm Sci 15:115-33. 2002
    ..Other co-solvent systems which do not freeze completely in commercial freeze-dryers were more difficult to use and often resulted in unacceptable freeze-dried cakes. Their use appears limited to levels of not more than 10%...
  36. ncbi Influenza vaccine powder formulation development: spray-freeze-drying and stability evaluation
    Yuh Fun Maa
    PowderJect Vaccines, Incorporated, 8551 Research Way Boulevard, Middleton, Wisconsin 53562, USA
    J Pharm Sci 93:1912-23. 2004
    ..More important, this study led to the selection of a formulation system that had been successfully tested in a human clinical study...
  37. pmc Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa
    H Kusakabe
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, HI 96822, USA
    Proc Natl Acad Sci U S A 98:13501-6. 2001
    ..The technique provides an effective means of storing mouse spermatozoa from many different inbred, mutant, and transgenic strains for biomedical research...
  38. ncbi Short- and long-term stability study of lyophilized solid lipid nanoparticles for gene therapy
    A del Pozo-Rodríguez
    Pharmacy and Pharmaceutical Technology Laboratory, University of the Basque Country UPV EHU, Vitoria Gasteiz, Spain
    Eur J Pharm Biopharm 71:181-9. 2009
    ..Furthermore, the storage of lyophilized lipoplexes stabilized with the disaccharide trehalose did not affect cell viability...
  39. ncbi Preparation of drug nanocrystals by controlled crystallization: application of a 3-way nozzle to prevent premature crystallization for large scale production
    Hans de Waard
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Groningen, The Netherlands
    Eur J Pharm Sci 38:224-9. 2009
    ..W., 2008. A novel bottom-up process to produce drug nanocrystals: controlled crystallization during freeze drying. J. Control. Release 128, 179-183]...
  40. ncbi Preservation of influenza virosome structure and function during freeze-drying and storage
    Jan Wilschut
    Department of Medical Microbiology, Molecular Virology Section, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    J Liposome Res 17:173-82. 2007
    ..It is concluded that freeze-drying in the presence of inulin as a lyoprotectant completely preserves the structure and function of influenza virosomes...
  41. ncbi Pure F-actin networks are distorted and branched by steps in the critical-point drying method
    Guenter P Resch
    Department of Cell Biology, Institute of Molecular Biology, Billrothstrasse 11, A 5020 Salzburg, Austria
    J Struct Biol 137:305-12. 2002
    ....
  42. ncbi Freeze-drying polymeric colloidal suspensions: nanocapsules, nanospheres and nanodispersion. A comparative study
    Scheila R Schaffazick
    Faculdade de Farmácia Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Eur J Pharm Biopharm 56:501-5. 2003
    ..In contrast to the literature, nanocapsules can be dried by freeze-drying without leakage of drug or breaking the capsule wall...
  43. ncbi Preparation and evaluation of lyophilized liposome-encapsulated bufadienolides
    Fang Li
    Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China
    Drug Dev Ind Pharm 35:1048-58. 2009
    ..The objective of this study was to prepare bufadienolides-loaded liposome (BU-lipo)...
  44. ncbi Long-term preservation of mouse spermatozoa after freeze-drying and freezing without cryoprotection
    Monika A Ward
    Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822, USA
    Biol Reprod 69:2100-8. 2003
    ..Both preservation methods are successful, but rapid freezing without cryoprotection is the preferred method for preservation of spermatozoa from mouse strains carrying unique genes and mutations...
  45. ncbi Freeze drying of human serum albumin (HSA) nanoparticles with different excipients
    Marion G Anhorn
    Institute of Pharmaceutical Technology, Biocenter of Johann Wolfgang Goethe University, Frankfurt, Germany
    Int J Pharm 363:162-9. 2008
    b>Freeze drying is a suitable technique to improve the long-term storage stability of colloidal drug carrier systems such as nanoparticles...
  46. ncbi Preparation and characterization of lyophilized liposomes with incorporated quercetin
    Elena Alexopoulou
    Department of Pharmaceutical Technology, School of Pharmacy, Panepistimiopolis Zografou, 15771 Athens, Greece
    J Liposome Res 16:17-25. 2006
    ....
  47. ncbi Effect of sugars on storage stability of lyophilized liposome/DNA complexes with high transfection efficiency
    Yoshie Maitani
    Institute of Medicinal Chemistry, Hoshi University, Ebara 2 4 41, Shinagawa ku, Tokyo 142 8501, Japan
    Int J Pharm 356:69-75. 2008
    ....
  48. ncbi Effect of lyophilization and storage temperature on the activity of salivaricin CRL 1328, a potential bioactive ingredient of a urogenital probiotic product
    Esteban Vera Pingitore
    Centro de Referencia para Lactobacilos CERELA CONICET, Chacabuco 145 T4000ILC San Miguel de Tucumán, Tucuman, Argentina
    J Gen Appl Microbiol 58:71-81. 2012
    ..This study provides useful information for the potential application of salivaricin as a bioactive principle for a pharmaceutical formulation...
  49. ncbi Preparation of submicron unilamellar liposomes by freeze-drying double emulsions
    Ting Wang
    Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, P O Box 52, No 103 Wenhua Road, Shenyang City, Liaoning Province 110016, PR China
    Biochim Biophys Acta 1758:222-31. 2006
    ..Thus, this reproducible and simple technique can be used to prepare sterilized, submicron unilamellar liposomes with a relatively high encapsulation efficiency, and excellent stability during long-term storage...
  50. ncbi The freezing step in lyophilization: physico-chemical fundamentals, freezing methods and consequences on process performance and quality attributes of biopharmaceuticals
    Julia Christina Kasper
    Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig Maximilians University, Munich, Germany
    Eur J Pharm Biopharm 78:248-63. 2011
    ....
  51. pmc Formulation of a dry powder influenza vaccine for nasal delivery
    Robert J Garmise
    School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    AAPS PharmSciTech 7:E19. 2006
    ..Further optimization of particle size and morphology is required for these powders to be suitable for clinical evaluation...
  52. pmc Stability of lyophilized siRNA nanosome formulations
    Anup K Kundu
    Center for Nanomedicine and Drug Delivery, Xavier University College of Pharmacy, New Orleans, LA 70125, United States
    Int J Pharm 423:525-34. 2012
    ..Thus, this study indicates that the stored lyophilized siRNA formulation is as effective as the fresh preparation and that long-term storage could be a viable option to treat deadly diseases such as cancer and viral infection...
  53. ncbi Development of stable lyophilized protein drug products
    Richard L Remmele
    Vaccine Formulation Development, MedImmune, 319 Bernardo Ave, Mountain View, CA 94043, USA
    Curr Pharm Biotechnol 13:471-96. 2012
    b>Freeze drying, or lyophilization is widely used for biopharmaceuticals to improve the long term storage stability of labile molecules. This review examines general theory and practice of rational lyophilization of biopharmaceuticals...
  54. pmc Comparison of spray freeze drying and the solvent evaporation method for preparing solid dispersions of baicalein with Pluronic F68 to improve dissolution and oral bioavailability
    Xiuqiong He
    Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
    AAPS PharmSciTech 12:104-13. 2011
    ..consisting of baicalein and a carrier with a low glass transition/melting point (Pluronic F68) by spray freeze drying (SFD). We compared these powders to those produced from the conventional solvent evaporation method...
  55. ncbi Lyophilized aspirin with trehalose may decrease the incidence of gastric injuries in healthy dogs
    Lee Shuan Lin
    Department of Veterinary Surgery, Graduate School of Agriculture and Life Science, The University of Tokyo, Tokyo, Japan
    J Vet Med Sci 74:1511-6. 2012
    ..Lyophilized aspirin/trehalose might be a solution to decrease aspirin-induced gastropathy...
  56. ncbi Preparation of redispersible dry nanocapsules by means of spray-drying: development and characterisation
    Patrice Tewa-Tagne
    Universite de Lyon, Lyon F 69003, France
    Eur J Pharm Sci 30:124-35. 2007
    ..The spray-drying technique is an attractive method to improve the NC conservation and facilitate future handling...
  57. ncbi Pharmacokinetics of desmopressin administrated as an oral lyophilisate dosage form in children with primary nocturnal enuresis and healthy adults
    Ole Osterberg
    Ferring Pharmaceuticals A S, Kay Fiskers Plads 11 DK 2300 Copenhagen S, Denmark
    J Clin Pharmacol 46:1204-11. 2006
    ..The main difference in the pharmacokinetics between children and adults was the absorption delay. The pharmacokinetic difference was minor and presumably of no clinical relevance...
  58. ncbi Freeze-drying of liposomes using tertiary butyl alcohol/water cosolvent systems
    JingXia Cui
    School of Pharmacy, Hebei Medical University, No 361, Zhongshan East Road, Shijiazhuang City, Hebei Province 050017, PR China
    Int J Pharm 312:131-6. 2006
    ..In addition, in conjunction with a modified injection method, this FD technology might be used to produce dehydrated HSPC liposomes on a large scale...
  59. ncbi Inulin sugar glasses preserve the structural integrity and biological activity of influenza virosomes during freeze-drying and storage
    Jørgen de Jonge
    Department of Medical Microbiology, Molecular Virology Section, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands
    Eur J Pharm Sci 32:33-44. 2007
    ..Formulation of influenza virosomes as a dry-powder is advantageous for storage and transport and offers the possibility to develop needle-free dosage forms, e.g. for oral, nasal, pulmonal, or dermal delivery...
  60. ncbi Aerosol delivery of nanoparticles in uniform mannitol carriers formulated by ultrasonic spray freeze drying
    Suzanne M D'Addio
    Advanced Drug Delivery Group, Faculty of Pharmacy, The University of Sydney, Pharmacy Building A15, Camperdown, New South Wales, 2006, Australia
    Pharm Res 30:2891-901. 2013
    ..We describe a successful dry powder formulation which is capable of delivering nanoparticles to the lungs with good aerosolization properties, high loadings of nanoparticles, and limited irreversible aggregation...
  61. ncbi Correlation of laboratory and production freeze drying cycles
    Wei Y Kuu
    BioPharma Solutions, Baxter Healthcare Corporation, Round Lake, IL 60073, USA
    Int J Pharm 302:56-67. 2005
    ..The cycle run for the LDH formulation using the Edwards production dryer verified that the cycle developed in a laboratory freeze-dryer was transferable at the production scale...
  62. ncbi Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells
    Tetsuo Ono
    Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology, Kobe, Japan
    J Reprod Dev 54:486-91. 2008
    ..Our findings show that the genomic integrity of cells can be maintained after freeze-drying and that it is possible to produce offspring from the cells using nuclear transfer techniques...
  63. ncbi Impact of matrix properties on the survival of freeze-dried bacteria
    Per Wessman
    Department of Microbiology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7025, SE 750 07 Uppsala, Sweden
    J Sci Food Agric 91:2518-28. 2011
    ..hydroxypropylmethylcellulose and polyvinylalcohol to support the survival of three bacterial strains during freeze drying. The initial osmotic conditions were adjusted to be similar for all formulations...
  64. ncbi Factors affecting short-term and long-term stabilities of proteins
    T Arakawa
    Amgen Inc, Amgen Center, Thousand Oaks, CA, USA
    Adv Drug Deliv Rev 46:307-26. 2001
    ..This review discusses the current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses, and also the various factors to be considered for long-term storage of proteins in solution...
  65. ncbi Survival of freeze-dried leuconostoc mesenteroides and Lactobacillus plantarum related to their cellular fatty acids composition during storage
    Ibourahema Coulibaly
    Unité des Bio industries, Centre Wallon de Biologie Industrielle, Faculte Universitaire des Sciences Agronomiques de Gembloux, Passage des Deportes, Gembloux, Belgium
    Appl Biochem Biotechnol 157:70-84. 2009
    ..mesenteroides and L. plantarum was noted in the freeze-dried powder held at 4 degrees C or under vacuum and in dark than at 20 degrees C or in the presence of oxygen and light...
  66. ncbi Effect of intracellular trehalose in Cryptococcus laurentii and exogenous lyoprotectants on its viability and biocontrol efficacy on Penicillium expansum in apple fruit
    B Q Li
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
    Lett Appl Microbiol 44:437-42. 2007
    To improve viability and biocontrol efficacy of Cryptococcus laurentii after freeze drying and in subsequent storage.
  67. ncbi First steps towards tissue engineering of small-diameter blood vessels: preparation of flat scaffolds of collagen and elastin by means of freeze drying
    L Buttafoco
    Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology and Institute of Biomedical Technology BMTI, University of Twente, Enschede, 7500AE, The Netherlands
    J Biomed Mater Res B Appl Biomater 77:357-68. 2006
    Porous scaffolds composed of collagen or collagen and elastin were prepared by freeze drying at temperatures between -18 and -196 degrees C. All scaffolds had a porosity of 90-98% and a homogeneous distribution of pores...
  68. ncbi Effect of culture age, protectants, and initial cell concentration on viability of freeze-dried cells of Metschnikowia pulcherrima
    Davide Spadaro
    Department of Exploitation and Protection of Agricultural and Forestry Resources, University of Turin, Grugliasco, TO, Italy
    Can J Microbiol 56:809-15. 2010
    ..pulcherrima cells was similar to that obtained in other studies. The results constitute a first step towards the commercial development of M. pulcherrima as a biocontrol agent...
  69. ncbi Survival curves for microbial species stored by freeze-drying
    Yukie Miyamoto-Shinohara
    International Patent Organism Depository, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Cryobiology 52:27-32. 2006
    ..The excellent survival after freeze-drying of each species might be attributable to the high level of desiccation and to sealing under vacuum...
  70. ncbi Freeze-drying fungi using a shelf freeze-drier
    C Shu hui Tan
    Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands
    Methods Mol Biol 368:119-25. 2007
    ..This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture collection (http://www.cbs.knaw.nl/)...
  71. ncbi Molecular cloning of mRNA from toad granular gland secretion and lyophilized skin: identification of Bo8--a novel prokineticin from Bombina orientalis
    Tianbao Chen
    Molecular Therapeutics Research Group, School of Pharmacy, Queen s University, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK
    Peptides 26:377-83. 2005
    ..Amino acid substitutions in skin prokineticins from the skin secretions of bombinid toads are confined to discrete sites affording the necessary information for structure/activity studies and analog design...
  72. ncbi Viability, efficacy, and storage stability of freeze-dried biocontrol agent Candida sake using different protective and rehydration media
    M Abadias
    Postharvest Unit, CeRTA, Centre UdL IRTA, Lleida, Catalonia, Spain
    J Food Prot 64:856-61. 2001
    ..The effect of several protective agents and rehydration media was investigated in the freeze drying of C. sake. Skimmed milk at 10% concentration was a good rehydration medium for all protectants tested...
  73. ncbi Unmasking venom gland transcriptomes in reptile venoms
    Tianbao Chen
    Pharmaceutical Biotechnology, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Ireland
    Anal Biochem 311:152-6. 2002
    ....
  74. pmc Lyophilization prior to direct DNA extraction from bovine feces improves the quantification of Escherichia coli O157:H7 and Campylobacter jejuni
    Delphine Rapp
    Climate, Land and Environment, AgResearch, East Street, Private Bag 3123, Hamilton 3240, New Zealand
    Appl Environ Microbiol 76:1686-8. 2010
    ....
  75. ncbi Tolerance of the mouse sperm nuclei to freeze-drying depends on their disulfide status
    Takehito Kaneko
    Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biol Reprod 69:1859-62. 2003
    ..The potential for freeze-drying damage seems likely to relate to the -SS- status of sperm proteins, in particular its protamines...
  76. ncbi Glycine betaine as a cryoprotectant for prokaryotes
    David Cleland
    Bacteriology Program, American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110, USA
    J Microbiol Methods 58:31-8. 2004
    ..These results indicate that GB is a simple and useful cryoprotectant that works for a wide range of prokaryotic organisms under different cryopreservation regimens...
  77. ncbi Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa
    In Kiu Kwon
    The Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Biol Reprod 71:1430-6. 2004
    ..These results demonstrate that pig oocytes can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst stage...
  78. ncbi Improvement in the long-term stability of freeze-dried mouse spermatozoa by adding of a chelating agent
    Takehito Kaneko
    Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, Kumamoto 860 0811, Japan
    Cryobiology 53:279-82. 2006
    ..This finding suggested that mouse spermatozoa can be freeze-dried in a simple solution containing the same composition as that used to preserve extracted DNA...
  79. ncbi Offspring derived from oocytes injected with rat sperm, frozen or freeze-dried without cryoprotection
    T Kaneko
    Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, 2 2 1 Honjo, Kumamoto, Japan
    Theriogenology 68:1017-21. 2007
    ..We concluded that this simple preservation method, in which cryoprotection was not required, allowed sperm to be preserved efficiently with maintenance of their fertilizing ability...
  80. ncbi Encapsulation and sustained release from biodegradable microcapsules made by emulsification/freeze drying and spray/freeze drying
    Weisi Yin
    Department of Chemical Engineering, University of Rochester, 206 Gavett Hall, Rochester, NY 14627, USA
    J Colloid Interface Sci 336:155-61. 2009
    Hollow biodegradable poly(DL-lactide) (PLA) particles with porous shell walls were prepared by freeze drying small droplets of PLA solution formed by emulsification or spraying...
  81. ncbi Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads
    Masumi Hirabayashi
    National Institute for Physiological Sciences, The Graduate University for Advanced Studies, Okazaki, Aichi, Japan
    Zygote 13:79-85. 2005
    ..Thus, the successful participation of freeze-dried spermatozoa in full-term development was demonstrated by applying ICSI in the rat...
  82. ncbi Importance of primary culture conditions for the development of rat ICSI embryos and long-term preservation of freeze-dried sperm
    Takehito Kaneko
    Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, Honjo, Kumamoto, Japan
    Cryobiology 58:293-7. 2009
    ..Also, rat sperm after freeze-drying in TE buffer were preserved at 4 degrees C for long term without their deterioration...
  83. ncbi Solid state fluorescence of lyophilized proteins
    Ranjini Ramachander
    Product and Process Development, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    Anal Biochem 376:173-82. 2008
    ..This suggests that solid state, intrinsic protein fluorescence measurements using the Cary-Eclipse holder may be feasible for long-term stability studies and formulation development...
  84. ncbi Constant size, variable density aerosol particles by ultrasonic spray freeze drying
    Suzanne M D'Addio
    Advanced Drug Delivery Group, Faculty of Pharmacy, The University of Sydney, Camperdown, NSW 2006, Australia
    Int J Pharm 427:185-91. 2012
    ..of critical process parameters involved in the production of inhalation aerosol particles by ultrasonic spray freeze drying to enable precise control over particle size and aerodynamic properties...
  85. pmc Controlled crystallization of the lipophilic drug fenofibrate during freeze-drying: elucidation of the mechanism by in-line Raman spectroscopy
    Hans de Waard
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, The Netherlands
    AAPS J 12:569-75. 2010
    ..In conclusion, for the model drug fenofibrate, a high freezing rate and a relatively low crystallization temperature resulted in the smallest crystals and therefore the highest dissolution rate...
  86. ncbi Preparation and pharmacokinetics of docetaxel based on nanostructured lipid carriers
    Xiang Li
    Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang, People s Republic of China
    J Pharm Pharmacol 61:1485-92. 2009
    ..This study describes the preparation and pharmacokinetics of docetaxel based on freeze-dried nanostructured lipid carriers (NLCs)...
  87. ncbi Influence of the stabilizer coating layer on the purification and freeze-drying of poly(D,L-lactic acid) nanoparticles prepared by an emulsion-diffusion technique
    D Quintanar Guerrero
    Interuniversity Centre Geneva Lyon, Archamps, France
    J Microencapsul 15:107-19. 1998
    In this study, the purification by cross-flow filtration (CFF) and freeze drying of poly(D,L-lactic acid) (PLA) nanoparticles prepared by an emulsion-diffusion technique using poly(vinyl alcohol) (PVAL) or poloxamer 188 (P-188) were ..
  88. ncbi Visualization of actin filaments in keratocyte lamellipodia: negative staining compared with freeze-drying
    J V Small
    Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg
    J Struct Biol 113:135-41. 1994
    ..Drying in negative stain appears to stabilise the actin network.(ABSTRACT TRUNCATED AT 250 WORDS)..
  89. ncbi Preparation and characterization of medium-chain fatty acid liposomes by lyophilization
    Chengmei Liu
    State Key Laboratory of Food Science and Technology, Department of Life Science and Food Engineering, Nanchang University, Nanchang, China
    J Liposome Res 20:183-90. 2010
    ..The mean diameters were 251.1 and 265.3 nm, and the encapsulation efficiencies were 80.5 and 79.2% for freshly prepared and reconstituted liposomes, respectively...
  90. ncbi Freeze-drying of nanocapsules: impact of annealing on the drying process
    Wassim Abdelwahed
    Laboratoire d Automatique et de Génie des Procédés LAGEP, UMR CNRS 5007, CPE Lyon, ISPB, Universite Claude Bernard Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
    Int J Pharm 324:74-82. 2006
    ..The acceleration of sublimation rate seems to depend on the temperature of annealing. The annealing of sucrose solution slows down the secondary drying kinetic whereas no effect is observed in the case of PVP...
  91. ncbi Freeze-dried primate sperm retains early reproductive potential after intracytoplasmic sperm injection
    Luis Gabriel Sánchez-Partida
    Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    Fertil Steril 89:742-5. 2008
    ..Oocytes inseminated by intracytoplasmic sperm injection using fresh ejaculated or freeze-dried rhesus macaque sperm showed similar activation, sperm aster assembly, and male-female pronuclear apposition rates...
  92. ncbi From anhydrobiosis to freeze-drying of eukaryotic cells
    Willem F Wolkers
    Department of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Comp Biochem Physiol A Mol Integr Physiol 131:535-43. 2002
    ..We suggest that this finding will obviate the storage problem with platelets and that the principles established here may be extended to more complex eukaryotic cells...
  93. pmc The stabilisation of purified, reconstituted P-glycoprotein by freeze drying with disaccharides
    Adam Heikal
    University of Brighton, School of Pharmacy and Biomolecular Sciences, Cockcroft Building, Lewes Road, Brighton BN2 4GJ, UK
    Cryobiology 58:37-44. 2009
    ..The ABCB1 purification procedure was optimised to allow successful freeze drying by substitution of glycerol with the disaccharides trehalose or maltose...
  94. ncbi Lyoprotected nanosphere formulations for paclitaxel controlled delivery
    T Musumeci
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Catania, V le A Doria, 6 I 95125 Catania, Italy
    J Nanosci Nanotechnol 6:3118-25. 2006
    ..The PLGA NS, in particular, showed a cell growth inhibitory activity comparable to the free drug...
  95. ncbi Protein spray-freeze drying. Effect of atomization conditions on particle size and stability
    H R Costantino
    Alkermes, Inc, Cambridge, MA 02139, USA
    Pharm Res 17:1374-83. 2000
    ..To investigate the effect of atomization conditions on particle size and stability of spray-freeze dried protein...
  96. pmc Development of stable influenza vaccine powder formulations: challenges and possibilities
    J P Amorij
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Groningen, The Netherlands
    Pharm Res 25:1256-73. 2008
    ..e. lyophilization, spray drying, spray-freeze drying, vacuum drying or supercritical fluid drying), the current status of dry influenza vaccine development and the ..
  97. ncbi Impaction bone grafting using freeze-dried allograft in revision hip arthroplasty
    N J de Roeck
    Orthopaedic Research Unit, East Surrey Hospital, Redhill, Surrey RH1 5RH, United Kingdom
    J Arthroplasty 16:201-6. 2001
    ..The results of impaction bone grafting with freeze-dried bone alone have been satisfactory, although we recommend caution in its use alone in the hostile acetabulum...
  98. ncbi Inulin glasses for the stabilization of therapeutic proteins
    W L Hinrichs
    Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands
    Int J Pharm 215:163-74. 2001
    ..When freeze drying is envisaged as method of drying, a relatively high Tg of the freeze concentrated fraction (Tg') is ..
  99. ncbi Instability, stabilization, and formulation of liquid protein pharmaceuticals
    W Wang
    Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
    Int J Pharm 185:129-88. 1999
    ....
  100. pmc Stability of dry liposomes in sugar glasses
    W Q Sun
    Department of Botany, National University of Singapore, Singapore
    Biophys J 70:1769-76. 1996
    ..The half-life of solute retention in dry liposomes may be prolonged by storing dry liposomes at temperatures below the Tg and by increasing the Tg of the dry liposome preparation...
  101. ncbi The relation between moisture-induced aggregation and structural changes in lyophilized insulin
    Giselle M Flores-Fernández
    Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, San Juan, Puerto Rico
    J Pharm Pharmacol 61:1555-61. 2009
    ..We have investigated the structural consequences of moisture sorption by lyophilized insulin under controlled humidity conditions by employing Fourier transform-infrared (FT-IR) microscopy...

Research Grants60

  1. Optical Coherence Tomography Based Freeze Drying Microscopy
    Mircea Mujat; Fiscal Year: 2010
    ..b>Freeze drying below Tc is necessary to insure elegant appearance, low residual water content and therefore good storage ..
  2. Development of stable and rapidly acting adjuvanted vaccines for biodefense
    ROBERT NEWTON BREY; Fiscal Year: 2013
    ..This is expected to result in dried thermostable subunit vaccines that can be given in an accelerated regimen (fewer numbers of doses) and which result in more rapid onset of protective immunity. ..
  3. Optical Coherence Tomography Based Freeze Drying Microscopy
    Mircea Mujat; Fiscal Year: 2013
    ..system to advance the understanding of freeze-drying and to enable the development of efficient commercial freeze drying processes...
  4. Genotype Analysis for Diagnosis of Urea Cycle Disorders
    Steven Dobrowolski; Fiscal Year: 2005
    ..Aim 4 will demonstrate gene scanning reagents (buffer, dye, dNTPs, oligonucleotides, enzyme) are stabilized by freeze drying and following re-suspension perform in a manner comparable to freshly prepared reagents...
  5. A Thermostable Measles Vaccine
    Jeffrey K Griffiths; Fiscal Year: 2010
    ..Vaccine campaigns have failed to eliminate measles because the vaccine goes bad when it is heated. Our project is to make a new vaccine that will not lose its ability to protect people if there is no refrigeration. ..
  6. YOUNG FROM STORED AND IMPAIRED MOUSE SPERM
    John Biggers; Fiscal Year: 2001
    ..Other studies will be concerned with improving the current methods of in vitro fertilization, intracytoplasmic sperm injection and embryo transfer in mice, the results of which will be incorporated in a final algorithm. ..
  7. Organic Solvent Vapor Mass Flow Rate Monitor for Pharmaceutical Drying Operations
    William J Kessler; Fiscal Year: 2012
    ..Example drying operations may include vacuum drying or fluid bed drying. Alternatively, freeze drying of labile drug products involves filling vials with liquid product (containing dissolved solids) and ..
  8. Freeze-Dried, Viable Allogeneic Heart Valves
    Kelvin Brockbank; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  9. Novel Osteoinductive Protein Synthesizing Implant System
    Kyumin Whang; Fiscal Year: 2003
    ....
  10. Revitalizing Allografts for Craniofacial Reconstruction
    Edward Schwarz; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  11. Process Analytic Technologies (PAT) for Biologics
    WILLIAM KESSLER; Fiscal Year: 2010
    ..abstract_text> ..
  12. DEVEL. OF A MINI-LYOPHILIZER FOR PHARMA. PRODUCTFORMUL.&PRO. &DEVEL. HHSN261201
    WILLIAM KESSLER; Fiscal Year: 2013
    ..The process of developing a drug formulation and freeze drying process begins with laboratory experiments followed by scale-up to pilot and production scale lyophilizers...
  13. CONCENTRATION METHOD FOR THERMALLY LABILE PHARMACEUTICAL
    JOHN BOWSER; Fiscal Year: 2000
    ..When product activity is sensitive to temperatures above room temperature, processes such as freeze drying are employed...
  14. STRUCTURE DETERMINATION OF THE NA/K-ATPASE
    Manijeh Mohraz; Fiscal Year: 1992
    ..It is closely related to Na, K-ATPase and the resulting structural similarities and differences could shed light on the mechanism of ion transport by these important biological systems...
  15. LYOPHILIZATION OF NONVIRAL GENE DELIVERY SYSTEMS
    THOMAS ANCHORDOQUY; Fiscal Year: 2002
    ..In addition to designing excipient formulations to stabilize lyophilized preparations, these studies will help to better characterize the "native state" of synthetic gene delivery systems. ..
  16. DIGESTION OF FOOD OXALATE
    Harmeet Sidhu; Fiscal Year: 2002
    ..The third specific aim will optimize fermentation and freeze drying conditions to produce a product with satisfactory cost, efficacy, safety and shelf life...
  17. Biochemical Stabilization for Freeze Dried Platelets
    Christopher Wagner; Fiscal Year: 2003
    ..abstract_text> ..
  18. Breaking Tolerance to a Tumor associated Antigen
    Scott Bingham; Fiscal Year: 2005
    ..We believe the novel delivery approach of this vaccine has potential to provide a broad immune response more capable of decreasing if not preventing tumor burden. ..
  19. NOVEL APPROACHES TO IMPROVE AND EXTEND PLATELET STORAGE
    Sherrill Slichter; Fiscal Year: 2001
    ....
  20. Stabilized Platelets for Advanced Wound Healing
    Cindy Orser; Fiscal Year: 2004
    ..loading methodologies, from endocytotic to peptide-mediated, will be evaluated for their applicability for pre-freeze drying loading...
  21. SIMPLE METHODS FOR SPERM CRYOPRESERVATION
    Ryuzo Yanagimachi; Fiscal Year: 2003
    ..based on our published and unpublished data which demonstrate that simple cryopreservation methods such as freeze drying can preserve the genetic material without the requirement to maintain sperm viability...
  22. Development of a highly efficient sperm freeze drying system
    Taylor Thompson; Fiscal Year: 2009
    ..Without this new technology, this priceless genetic resource could be lost and become unavailable for lifesaving research in the future. ..
  23. CONCENTRATION METHOD FOR THERMALLY LABILE PHARMACEUTICAL
    JOHN BOWSER; Fiscal Year: 2004
    ....
  24. Formulation of Synthetic Vectors as Lyophilized Products
    THOMAS ANCHORDOQUY; Fiscal Year: 2009
    ..g., vaccines, viruses, nanoparticles) during freeze-drying and storage. ..
  25. REGULATION OF PLATELET SHAPE CHANGES
    John Hartwig; Fiscal Year: 1993
    ..We propose a comprehensive interdisciplinary study on to define the resting platelet cytoskeleton and understand the mechanism of platelet actin assembly during activation of the cells...
  26. Chemoprevention of Esophageal Squamous Cell Carcinoma
    Gary Stoner; Fiscal Year: 2004
    ..abstract_text> ..
  27. METABOLISM OF CARCINOGENIC TOBACCO SPECIFIC NITROSAMINES
    Stephen Hecht; Fiscal Year: 2003
    ..abstract_text> ..
  28. Cellular Remodeling of ECM Scaffolds
    Stephen Badylak; Fiscal Year: 2009
    ..We have identified clearly defined specific aims with quantifiable end points and an efficient three year timeline to conduct this work. ..
  29. Development of Hydrogel Spinal Disc Replacement
    Barbara Boyan; Fiscal Year: 2004
    ..abstract_text> ..
  30. Nano Fibers for ADME/Toxicology
    Peter Ma; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  31. Targeting Histone Deacetylation: A Tool for Chemopevention of Esophageal Cancer
    Laura Kresty; Fiscal Year: 2008
    ..goal of identifying and translating efficacious preventive agents into the clinic for evaluation in high risk human cohorts, including patients with long standing Barrett's esophagus or [unreadable] [unreadable] [unreadable]..
  32. BIOLOGY OF PROTEOLYTIC DERIVATIVES OF LP(A)
    ANGELO SCANU; Fiscal Year: 2009
    ..The results of the proposed studies should shed light on whether ox-PL adducts play a role in the cardiovascular pathogenicity of Lp(a). ..
  33. Development and Characterization of a Novel Contrast Agent
    Margaret Wheatley; Fiscal Year: 2009
    ..A diagnostic tool that could safely and non-invasively detect tumors at an early stage, before metastasis, would be a great advance in health care. ..
  34. Orchestrating Bone Regeneration on Multiple Scales
    Peter X Ma; Fiscal Year: 2010
    ..SA 3. Confirm that the multi-scaled and factor-releasing scaffolds (selected from Aims 1&2) afford a superior environment for vascularized bone regeneration in a rat critical defect model. ..
  35. INTERACTIONS OF LP(A) WITH VASCULAR EXTRACELLULAR MATRIX
    ANGELO SCANU; Fiscal Year: 2002
    ..Underlying these studies is the hypothesis, that the MMP-mediated changes in Lp(a)/apo(a) are favored by the inflammatory milieu of the human atheroma. ..
  36. CARCINOGENIC CYCLIC NITROSAMINE DNA ADDUCTS
    Stephen S Hecht; Fiscal Year: 2010
    ..The proposed research is significant because of the known human exposure to these carcinogens through the diet, tobacco products, and endogenous formation. ..
  37. Cell Recruitment Induced by ECM Scaffold Degradation
    Stephen F Badylak; Fiscal Year: 2010
    ..The objectives of the studies, the hypothesis upon which the experimental design is based, and a timeline for completion of the studies are presented. ..
  38. BONE FACTORS AND ENDOCHONDRAL OSSIFICATION
    Barbara Boyan; Fiscal Year: 2001
    ..It is suggested that these results will provide insight into the role these factors play in bone development and repair. ..
  39. ROLE OF MEMBRANES AND MINERALIZATION OF BONES AND TEETH
    Barbara Boyan; Fiscal Year: 2001
    ..This work will have major implications for our understanding of MV and how cells that calcify their matrix are able to regulate these extracellular organelles at distant sites. ..
  40. Lung Cancer Chemoprevention: Isothiocyanates, inositol
    Stephen Hecht; Fiscal Year: 2008
    ..abstract_text> ..
  41. Gamma-Delta T cells and Cryptosporidiosis
    Beth Kirkpatrick; Fiscal Year: 2008
    ..These data will advance our understanding of the mucosal immune response to cryptosporidiosis, which will lead to further advances in prevention and therapy. ..
  42. Contrast Enhanced Ultrasound with Therapeutic Modality
    Margaret Wheatley; Fiscal Year: 2004
    ..These studies are a prelude to show feasibility prior to in vivo studies. ..
  43. Influence of Sex and Gender on Musculoskeletal Health
    Barbara Boyan; Fiscal Year: 2004
    ..Worldwide current and future clinical trials will be discussed. Short papers on the workshop topics, future directions, and scientific updates will be published via Internet and summary papers in peer-reviewed journals. ..
  44. Chemoprevention of Oral Dysplasia by Black Raspberries
    Susan Mallery; Fiscal Year: 2005
    ..This experimental design (comparison of pretreatment and post-treatments tissues) permits each patient to serve as their own internal control. ..
  45. Biomimetic Nano Scaffolds
    Peter Ma; Fiscal Year: 2004
    ..This knowledge will enable us to develop a predictable biomimetic scaffold for in vivo application and will be the basis for our planned R01 investigation. ..
  46. Preservation of ejaculated mouse spermatozoa
    MONIKA WARD; Fiscal Year: 2006
    ..Our expectations are that the results of this application will allow us to recommend ICSI with preserved ejaculated spermatozoa as a simple and efficient method for preservation of valuable mouse genomes. ..
  47. Tobacco Carcinogenesis
    Stephen Hecht; Fiscal Year: 2005
    ..Steven Tannenbaum (MIT), Dr. Jack Henningfield (Johns Hopkins and Pinney Associates), Drs. Jeffrey Seeman and Anthony Tricker (Philip Morris), and Dr. John Lauterbach (an independent consultant, formerly with Brown and Williamson). ..
  48. NanobioReactor for monitoring small cell populations
    Ales Prokop; Fiscal Year: 2003
    ..abstract_text> ..
  49. Mechanisms of ECM Scaffold Remodeling
    Stephen Badylak; Fiscal Year: 2006
    ..The work will be conducted by an experienced interdisciplinary team of scientists and a timeline is presented for completion of the work. ..
  50. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
    ..are treated with various chemicals (detergents, DTT, exogenous DNA) or are subjected to mechanical insults (freeze drying and freezing without cryoprotection), and then injected into the oocytes, the paternal chromosomes in the ..
  51. The Role of VDR in PTH and Vitamin D3(D3) Synergy
    Tara Aghaloo; Fiscal Year: 2008
    ..These studies will help understand the mechanisms of PTH and D3 synergy through VDR and their effects on osteoblastic differentiation and function. [unreadable] [unreadable] [unreadable]..
  52. Ice Nucelation in Biological Tissues - Implications to Cryopreservation
    Ram Devireddy; Fiscal Year: 2007
    ..SA2: Extension of the technique to measure ice nucleation kinetics in whole tissues. SA3: Modeling and prediction of ice nucleation kinetics in whole tissue sections. [unreadable] [unreadable] [unreadable]..
  53. Bacterial Vaccine Antigen Discovery
    LAWRENCE PAOLETTI; Fiscal Year: 2006
    ..Aim 2). Findings from these studies utilizing this unique approach to vaccine antigen discovery could be directly applied to other bacterial pathogens. ..
  54. Mechanobiology and Regenerative Medicine
    Michael S Sacks; Fiscal Year: 2010
    ..A timeline for completion of these studies, hypotheses to drive the specific aims, alternative approaches for completion of the work, and quantitative criteria for success are provided. ..
  55. Development of a Global GBS Vaccine
    LAWRENCE PAOLETTI; Fiscal Year: 2007
    ..This work will lead to a vaccine effective in preventing GBS diseases worldwide. ..
  56. Role of Oxidants & Angiogenesis in Kaposi's Sarcoma
    Susan Mallery; Fiscal Year: 2007
    ..Elucidation of these interactions will not only clarify KS pathogenic mechanisms, but will also identify sites for KS therapeutic intervention. ..
  57. Formulation & Evaluation of Bioadhesive Gels for Oral Cancer Chemoprevention
    Susan Mallery; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  58. ORIGIN OF CELLS THAT REPOPULATE RESORBABLE ECM SCAFFOLDS
    Stephen Badylak; Fiscal Year: 2003
    ..abstract_text> ..
  59. ORIGIN OF CELLS THAT REPOPULATE RESORBABLE ECM SCAFFOLDS
    Stephen Badylak; Fiscal Year: 2001
    ..abstract_text> ..