cryopreservation

Summary

Summary: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.

Top Publications

  1. ncbi Cryopreservation and autotransplantation of human ovarian tissue prior to cytotoxic therapy--a technique in its infancy but already successful in fertility preservation
    Michael von Wolff
    Department of Gynecological Endocrinology and Reproductive Medicine, University of Bern, Women s Hospital, Inselspital, Effingerstrasse 102, Bern 3010, Switzerland
    Eur J Cancer 45:1547-53. 2009
  2. ncbi The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissue
    Erik Ernst
    Department of Gynecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark
    Hum Reprod 25:1280-1. 2010
  3. ncbi The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure
    Shinsuke Seki
    Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996 0840, USA
    Cryobiology 59:75-82. 2009
  4. ncbi Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification
    Yun Xia Cao
    Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, People s Republic of China
    Fertil Steril 92:1306-11. 2009
  5. ncbi Storage of human oocytes in the vapor phase of nitrogen
    Ana Cobo
    IVI Universidad de Valencia, Valencia, Spain
    Fertil Steril 94:1903-7. 2010
  6. ncbi Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification
    Gary D Smith
    Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA
    Fertil Steril 94:2088-95. 2010
  7. ncbi Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue
    Claus Yding Andersen
    Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Hum Reprod 23:2266-72. 2008
  8. ncbi Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units
    A Bielanski
    Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa Nepean, ON, Canada K2H 8P9
    Hum Reprod 24:2457-67. 2009
  9. ncbi American Society of Clinical Oncology recommendations on fertility preservation in cancer patients
    Stephanie J Lee
    Dana-Farber Cancer Institute, Boston, MA, USA
    J Clin Oncol 24:2917-31. 2006
  10. ncbi Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis
    L K Thomson
    Fertility First, PO Box 807, Hurstville, NSW 2220, Australia
    Hum Reprod 24:2061-70. 2009

Research Grants

  1. CYROBIOLOGICAL PRESERVATION OF ANOPHELES EMBRYOS
    Peter Mazur; Fiscal Year: 2001
  2. ICE-FREE PRESERVATION OF OVARIES FOR LONG-TERM FECUNDITY
    Ying Song; Fiscal Year: 2004
  3. Lyophilization of Plasmodium falciparum sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2007
  4. A Novel Testicular Germ Cell Maturation Model for Fertility Preservation in Pre-p
    Kirk C Lo; Fiscal Year: 2010
  5. HEPTOCELLULAR RESPONCE TO CRYOPRESERVATION
    Mehmet Toner; Fiscal Year: 2002
  6. ORGANIZATION AND FUNCTIONS OF LIPID RAFTS IN SPERMATOZOA
    Alexander Travis; Fiscal Year: 2007
  7. PROTOCOLS FOR MATURING AND FREEZING OOCYTES
    Catherine VandeVoort; Fiscal Year: 2001
  8. PRESERVATION OF GENETICS LINES IN A NON-MAMMALIAN SYSTEM
    Mary Hagedorn; Fiscal Year: 2001
  9. Letrozole Therapy for IVF/ET: Effects on Multiple Pregnancy and Treatment Burden
    Robert Brzyski; Fiscal Year: 2007
  10. Potency of cryopreserved, irradiated sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2005

Detail Information

Publications281 found, 100 shown here

  1. ncbi Cryopreservation and autotransplantation of human ovarian tissue prior to cytotoxic therapy--a technique in its infancy but already successful in fertility preservation
    Michael von Wolff
    Department of Gynecological Endocrinology and Reproductive Medicine, University of Bern, Women s Hospital, Inselspital, Effingerstrasse 102, Bern 3010, Switzerland
    Eur J Cancer 45:1547-53. 2009
    ..Several techniques are now available for fertility preservation in these patients. A new promising method is cryopreservation and transplantation of ovarian cortex...
  2. ncbi The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissue
    Erik Ernst
    Department of Gynecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark
    Hum Reprod 25:1280-1. 2010
    ..of these children were born in Denmark following transport of the ovarian tissue for a period of 5 h prior to cryopreservation. One of these women, who was originally transplanted with six pieces of ovarian cortex, after having ..
  3. ncbi The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure
    Shinsuke Seki
    Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996 0840, USA
    Cryobiology 59:75-82. 2009
    ..The two contrasting routes to avoiding it are slow equilibrium freezing and vitrification. The cryopreservation of mammalian oocytes by either method continues to be difficult, but there seems a slowly emerging consensus ..
  4. ncbi Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification
    Yun Xia Cao
    Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, People s Republic of China
    Fertil Steril 92:1306-11. 2009
    ..To compare the survival, fertilization, early embryonic development, and meiotic spindle assembly and chromosome alignment in frozen-thawed human oocytes after slow-freezing and vitrification...
  5. ncbi Storage of human oocytes in the vapor phase of nitrogen
    Ana Cobo
    IVI Universidad de Valencia, Valencia, Spain
    Fertil Steril 94:1903-7. 2010
    ..To evaluate the effectiveness of long-term vapor-phase nitrogen storage of vitrified human oocytes as a strategy for preventing the risk of cross-contamination due to direct contact with the liquid nitrogen (LN)...
  6. ncbi Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification
    Gary D Smith
    Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA
    Fertil Steril 94:2088-95. 2010
    To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy.
  7. ncbi Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue
    Claus Yding Andersen
    Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Hum Reprod 23:2266-72. 2008
    b>Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease...
  8. ncbi Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units
    A Bielanski
    Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa Nepean, ON, Canada K2H 8P9
    Hum Reprod 24:2457-67. 2009
    b>Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies...
  9. ncbi American Society of Clinical Oncology recommendations on fertility preservation in cancer patients
    Stephanie J Lee
    Dana-Farber Cancer Institute, Boston, MA, USA
    J Clin Oncol 24:2917-31. 2006
    ..Sperm and embryo cryopreservation are considered standard practice and are widely available; other available fertility preservation methods ..
  10. ncbi Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis
    L K Thomson
    Fertility First, PO Box 807, Hurstville, NSW 2220, Australia
    Hum Reprod 24:2061-70. 2009
    Whereas studies have revealed that the cryopreservation of human semen increases sperm DNA fragmentation, the mechanisms involved in this type of cryo-injury are largely unknown...
  11. ncbi Highly efficient vitrification method for cryopreservation of human oocytes
    Masashige Kuwayama
    Kato Ladies Clinic, Tokyo, Japan
    Reprod Biomed Online 11:300-8. 2005
    ..methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0...
  12. ncbi Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials
    Marta Bull
    Children s Hospital and Regional Medical Center, Seattle, WA, USA
    J Immunol Methods 322:57-69. 2007
    ..function of the resulting cells, including anticoagulant type, time from venipuncture to PBMC isolation/cryopreservation, method of PBMC isolation and procedure for sample shipping...
  13. ncbi Xenotransplantation of cryopreserved ovarian tissue from patients with ovarian tumors into SCID mice--no evidence of malignant cell contamination
    Laura Lotz
    Department of Obstetrics and Gynecology, Erlangen University Hospital, Erlangen, Germany
    Fertil Steril 95:2612-4.e1. 2011
    ..None of the mice presented symptoms of reintroduced malignancy nor did microscopic and immunohistochemical evaluation of the grafts raise any suspicion of residual malignant disease...
  14. ncbi Micro-organ ovarian transplantation enables pregnancy: a case report
    Ariel Revel
    Department of Obstetrics and Gynecology, Hadassah Medical Center and Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Hum Reprod 26:1097-103. 2011
    ..We report that preparation of autologous tiny ovarian fragments (MO)s and reimplantation into our patient resulted in IVF pregnancy and delivery of a healthy baby...
  15. ncbi Isolation of pre-antral follicles from human ovarian medulla tissue
    Stine Gry Kristensen
    Copenhagen University Hospital Rigshospitalet, Copenhagen University, København Ø, Denmark
    Hum Reprod 26:157-66. 2011
    b>Cryopreservation of ovarian tissue for fertility preservation is based on the ovarian cortex that contains the vast majority of the follicular reserve, while the remaining tissue, the medulla is discarded...
  16. ncbi Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis
    Kalliopi E Loutradi
    Unit for Human Reproduction, 1st Department of Obstetrics and Gynaecology, Papageorgiou General Hospital, Thessaloniki, Greece
    Fertil Steril 90:186-93. 2008
    ..the literature systematically in order to identify prospective comparative trials answering the following question: Is vitrification of human embryos associated with a higher postthawing survival rate as compared with slow freezing?..
  17. ncbi The efficacy and safety of human oocyte vitrification
    Zsolt Peter Nagy
    Reproductive Biology Associates, Atlanta, Georgia 30342, USA
    Semin Reprod Med 27:450-5. 2009
    Vitrification is now a widely applied and highly successful approach for cryopreservation in reproductive biology. Rapidly increasing data prove that it is also a highly efficient technique for low-temperature storage of human oocytes...
  18. ncbi Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique
    Mikkel Rosendahl
    Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Denmark
    Reprod Biomed Online 22:162-71. 2011
    This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated...
  19. ncbi Effect of cryopreservation on sperm DNA integrity in patients with teratospermia
    Guruprasad Kalthur
    Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India
    Fertil Steril 89:1723-7. 2008
    ..To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing...
  20. ncbi Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafe
    Marie Madeleine Dolmans
    Department of Gynecology, GYNE Research Unit, Institut de Recherche Expérimentale et Clinique, Universite Catholique de Louvain, Brussels, Belgium
    Blood 116:2908-14. 2010
    Ovarian tissue cryopreservation is currently proposed to young cancer patients to preserve their fertility before radiochemotherapy. The potential risk is that the tissue might harbor malignant cells that could induce disease recurrence...
  21. ncbi A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation
    B Balaban
    Assisted Reproduction Unit, American Hospital of Istanbul, Guzelbahce Sokak 20, Nisantasi, Istanbul 34365, Turkey
    Hum Reprod 23:1976-82. 2008
    The aim of this study was to compare two methods of cryopreservation for the cleavage-stage human embryo: slow freezing and vitrification.
  22. ncbi Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos
    Lodovico Parmegiani
    Reproductive Medicine Unit, GynePro Medical Centers, Bologna, Italy
    Fertil Steril 94:1525-8. 2010
    ..To evaluate the efficacy of ultraviolet (UV) irradiation for rapid microbial decontamination of liquid nitrogen (LN2)...
  23. ncbi Cryopreservation of human embryos by vitrification or slow freezing: which one is better?
    Efstratios M Kolibianakis
    First Department of Obstetrics and Gynaecology, Unit for Human Reproduction, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
    Curr Opin Obstet Gynecol 21:270-4. 2009
    To summarize the available evidence from randomized controlled trials comparing vitrification versus slow freezing for cryopreservation of human embryos.
  24. ncbi Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM
    P Vanderzwalmen
    IVF Centers Prof Zech, Bregenz, Austria
    Reprod Biomed Online 19:700-7. 2009
    ..In spite of reduced cooling rates due to aseptic vitrification conditions, a three-step exposure to cryoprotectant solutions protects the embryos effectively from cryo-injuries and guaranties high survival rates...
  25. ncbi Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle
    R Soleimani
    Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Hum Reprod 25:1458-70. 2010
    Ovarian tissue (OT) cryopreservation and transplantation are options for fertility preservation in young female cancer patients.
  26. ncbi Preservation of fertility in females with haematological malignancy
    Jacques Donnez
    Department of Gynecology, Universite Catholique de Louvain, Brussels, Belgium
    Br J Haematol 154:175-84. 2011
    Several options are currently available for the preservation of fertility in cancer patients: embryo cryopreservation, oocyte cryopreservation or ovarian tissue cryopreservation...
  27. ncbi Orthotopic and heterotopic ovarian tissue transplantation
    I Demeestere
    Research Laboratory on Human Reproduction, Medicine Faculty, Universite Libre de Bruxelles ULB, Erasme Hospital, 808 Route de Lennik, 1070 Brussels, Belgium
    Hum Reprod Update 15:649-65. 2009
    ..Here, we review the different factors affecting follicular survival and growth after grafting...
  28. ncbi Live birth after vitrification of in vitro matured human oocytes
    Ri Cheng Chian
    McGill Reproductive Centre, McGill University Health Centre, McGill University, Montreal, Quebec, Canada
    Fertil Steril 91:372-6. 2009
    To report the first healthy live birth from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM) and cryopreservation of the oocytes by vitrification.
  29. ncbi Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants
    Prudencio B Pedro
    Laboratory of Animal Science, College of Agriculture, Kochi University, Japan
    J Reprod Dev 51:235-46. 2005
    ..Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development.
  30. ncbi Analysis of oocyte physiology to improve cryopreservation procedures
    David K Gardner
    Colorado Center for Reproductive Medicine, Englewood, CO 80113, USA
    Theriogenology 67:64-72. 2007
    ..Differences in membrane permeability and in physiology are two main reasons why successful oocyte cryopreservation has remained elusive...
  31. ncbi Gene expression profiling of formalin-fixed, paraffin-embedded familial breast tumours using the whole genome-DASL assay
    Nic Waddell
    The Queensland Institute of Medical Research, Brisbane, Australia
    J Pathol 221:452-61. 2010
    ....
  32. ncbi Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates
    Gottumukkala Achyuta Rama Raju
    Embryology Division, Krishna IVF Clinic, Zillaparishad Junction, Visakhapatnam, Pincode 530002, Andhra Pradesh, India
    Reprod Biomed Online 11:434-7. 2005
    ..Ethylene glycol is a good cryoprotectant to preserve human 8-cell embryos because of its low toxicity as shown by the high survival rate, and vitrification is a promising alternate to the conventional slow-freezing method...
  33. ncbi At what age can human oocytes be obtained?
    Ariel Revel
    Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    Fertil Steril 92:458-63. 2009
    ..To determine whether oocyte retrieval and in vitro maturation (IVM) is effective in girls undergoing fertility preservation before cancer treatment...
  34. ncbi Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method
    B E Reubinoff
    Department of Obstetrics and Gynecology, Goldyn Savad Institute of Gyne Therapy, Hadassah University Hospital, Ein Kerem, Jerusalem 91120, Israel
    Hum Reprod 16:2187-94. 2001
    ..The study aim was to determine whether the open pulled straw (OPS) vitrification method, which is highly effective for the cryopreservation of embryos, might be also efficient for human ES cells.
  35. ncbi Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue
    Victoria Keros
    Department of Clinical Science, Technology and Intervention, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, K 57, Stockholm SE 141 86, Sweden
    Hum Reprod 24:1670-83. 2009
    ..To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus ..
  36. ncbi An efficient method for the rapid establishment of Epstein-Barr virus immortalization of human B lymphocytes
    H-M Oh
    Department of Microbiology and Immunology, Wonkwang University School of Medicine, Chonbuk, Korea
    Cell Prolif 36:191-7. 2003
    ..We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester-induced EBV stock significantly improved ..
  37. ncbi Dendritic cell immunotherapy for urological cancers using cryopreserved allogeneic tumour lysate-pulsed cells: a phase I/II study
    Hardev S Pandha
    Department of Histopathology, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
    BJU Int 94:412-8. 2004
    ..To assess the feasibility, toxicity and immunogenicity of dendritic cell (DC)-based immunotherapy in patients with advanced urological cancers...
  38. ncbi Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC
    Lisa S Kierstead
    Vaccine and Biologics Research, Merck Research Laboratories, West Point and Wayne, PA 19087, USA
    AIDS Res Hum Retroviruses 23:86-92. 2007
    ..These studies establish the importance of optimal conditions for PBMC collection and handling to the success of a clinical development program...
  39. ncbi Fertility preservation in women--a practical guide to preservation techniques and therapeutic strategies in breast cancer, Hodgkin's lymphoma and borderline ovarian tumours by the fertility preservation network FertiPROTEKT
    Michael von Wolff
    Department of Gynaecological Endocrinology and Reproductive Medicine, University Women s Hospital, Effingerstrasse 102, 3010 Bern, Switzerland
    Arch Gynecol Obstet 284:427-35. 2011
    ..However, balanced practical recommendations concerning all relevant fertility preservation, to support doctors in counselling and treating patients, are still rare...
  40. ncbi Ultra-rapid vitrification of mouse oocytes in low cryoprotectant concentrations
    Ho Joon Lee
    Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Reprod Biomed Online 20:201-8. 2010
    The ideal cryopreservation protocol would combine the benefits of slow freezing with the benefits of vitrification. This report describes a method for the ultra-rapid vitrification of oocytes using slush nitrogen in quartz capillaries...
  41. ncbi Autotransplantation of cryopreserved ovarian tissue in 12 women with chemotherapy-induced premature ovarian failure: the Danish experience
    Kirsten Tryde Schmidt
    The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    Fertil Steril 95:695-701. 2011
    ..To describe a cohort of 12 Danish women who received autotransplantation of frozen-thawed cryopreserved ovarian tissue because of premature ovarian failure after cancer treatment...
  42. ncbi Fertility preservation for young patients with cancer: who is at risk and what can be offered?
    W Hamish B Wallace
    Department of Child Life and Health, Division of Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh, UK
    Lancet Oncol 6:209-18. 2005
    ....
  43. ncbi Motility, acrosome integrity, membrane integrity and oocyte cleavage rate of sperm separated by swim-up or Percoll gradient method from frozen-thawed buffalo semen
    A Mehmood
    Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
    Anim Reprod Sci 111:141-8. 2009
    ..05), while the Percoll gradient method did not. It has been concluded that separation of sperm from frozen-thawed buffalo semen by swim-up method can be more expedient for IVF in buffalo...
  44. ncbi Sterilisation of skin allograft with gamma irradiation
    P Rooney
    National Health Service Blood and Transplant, Tissue Services, Estuary Banks, Liverpool L24 8RB, UK
    Burns 34:664-73. 2008
    ..These non-viable grafts can be made available for surgical use when cryopreserved viable grafts are not available or required...
  45. ncbi Fertility preservation: successful transplantation of cryopreserved ovarian tissue in a young patient previously treated for Hodgkin's disease
    Isabelle Demeestere
    Research Laboratory on Human Reproduction, Campus Erasme Bat GE CP636, 808 Route de Lennik, 1070, Brussels, Belgium
    Oncologist 12:1437-42. 2007
    b>Cryopreservation of ovarian tissue is now offered as an experimental procedure to preserve the fertility of young patients with a high risk for premature ovarian failure resulting from cancer therapy...
  46. ncbi Alginate encapsulation as a novel strategy for the cryopreservation of neurospheres
    Rita Malpique
    Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Tissue Eng Part C Methods 16:965-77. 2010
    ..Such applications would greatly benefit from the development of efficient cryopreservation protocols that assure the availability of viable and genetically stable stocks of functional neurospheres...
  47. ncbi Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification
    Joseph Saragusty
    Department of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, Alfred Kowalke Strasse 17, Berlin, Germany
    Reproduction 141:1-19. 2011
    Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and ..
  48. ncbi A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells
    Sepideh Mollamohammadi
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, PO Box 19395 4644, Tehran, Iran
    Hum Reprod 24:2468-76. 2009
    An essential prerequisite for the future widespread application of human induced pluripotent (hiPSCs) and embryonic stem cells (hESCs) is the development of efficient cryopreservation methods to facilitate their storage and transportation.
  49. ncbi Changes in sperm quality and lipid composition during cryopreservation of boar semen
    A Maldjian
    Bio Innovations, Southburn, East Yorkshire, YO25 9ED, UK
    Theriogenology 63:411-21. 2005
    ..concerns the eventual exchanges of lipid components between the spermatozoa and the yolk-based diluent during cryopreservation. Two types of yolk have been considered as ingredients in diluents for cryopreservation: yolks with a ..
  50. ncbi Symposium: Update on prediction and management of OHSS. Prevention of OHSS
    Mohamed Aboulghar
    Cairo University and the Egyptian IVF Center, Maadi, Cairo, Egypt
    Reprod Biomed Online 19:33-42. 2009
    ..Intravenous albumin is useful in the prevention when given at time of oocyte retrieval. Cryopreservation of all embryos will reduce late-onset OHSS but not early-onset OHSS...
  51. ncbi The effect of cryopreservation or heating on the mechanical properties and histomorphology of rat bone-patellar tendon-bone
    Hwang Jung Park
    Department of Orthopaedic Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
    Cell Tissue Bank 10:11-8. 2009
    The effects of cryopreservation on tendon allograft have been reported, but remain unclear, particularly the potential effects on mechanical properties and histological changes by ice crystal formation...
  52. ncbi Effect of different cryopreservation protocols on the metaphase II spindle in human oocytes
    A Cobo
    IVI Universidad de Valencia Plaza de la Policía local, 3 46015 Valencia, Spain
    Reprod Biomed Online 17:350-9. 2008
    The aim of this study was to evaluate the impact of different cryopreservation protocols on the repolymerization of metaphase (M)II spindles in human oocytes...
  53. ncbi Ovarian cryopreservation for fertility preservation: indications and outcomes
    R A Anderson
    The Queen s Medical Research Institute, Centre for Reproductive Biology, University of Edinburgh, Edinburgh, UK
    Reproduction 136:681-9. 2008
    ..provides significantly different challenges to that for the male, with the only established method being cryopreservation of embryos thus necessitating the involvement of a male...
  54. ncbi Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa
    G Martin
    Laboratoire de Biologie de la Reproduction GIMAP, Hopital Nord, Saint Etienne, France
    Hum Reprod 22:380-8. 2007
    b>Cryopreservation/thawing of bovine spermatozoa induces a reduction in cell viability and is possibly associated with a form of programmed cell death that we previously named 'apoptosis-like phenomenon'.
  55. ncbi New options in assisted reproduction technology: the Cryotop method of oocyte vitrification
    Ana Cobo
    IVI Universidad de Valencia, Plaza de la Policia Local, 3 46015 Valencia, Spain
    Reprod Biomed Online 17:68-72. 2008
    The Cryotop vitrification method has been shown to be a very useful tool for oocyte cryopreservation, giving excellent results regarding survival and clinical outcome...
  56. ncbi An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples
    Xiao Zhang
    Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
    J Mol Diagn 10:513-9. 2008
    ..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...
  57. ncbi Report of four donor-recipient oocyte cryopreservation cycles resulting in high pregnancy and implantation rates
    Jason Barritt
    Department of Obstetrics and Gynecology, and Department of Reproductive Endocrinology and Infertility, Mount Sinai School of Medicine, New York, New York, USA
    Fertil Steril 87:189.e13-7. 2007
    To determine the clinical potential of donor-oocyte cryopreservation and thaw techniques for recipient patients.
  58. ncbi Clinical-grade ex vivo-expanded human natural killer cells up-regulate activating receptors and death receptor ligands and have enhanced cytolytic activity against tumor cells
    Maria Berg
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Cytotherapy 11:341-55. 2009
    ..We provide a method for expanding highly cytotoxic clinical-grade NK cells in vitro for adoptive transfer following bortezomib treatment in patients with advanced malignancies...
  59. ncbi Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll. Arg.)
    Ludovic Lardet
    CIRAD CP, UMR 1098, TA 80 03, Avenue Agropolis, 34398 Montpellier, France
    Plant Cell Rep 26:559-69. 2007
    A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis...
  60. ncbi Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio
    Huiping Yang
    Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
    Theriogenology 68:128-36. 2007
    Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible...
  61. ncbi Integrated morphophysiological assessment of two methods for sperm selection in bovine embryo production in vitro
    A Cesari
    Instituto de Investigaciones Biologicas, Facultad de Ciencias Exactas y Naturales, UNMdP CC 1245 7600 Mar del Plata, Buenos Aires, Argentina
    Theriogenology 66:1185-93. 2006
    ....
  62. ncbi Morphological and ultrastructural evaluation of cultured frozen-thawed human fetal ovarian tissue
    Jean Clair Sadeu
    Follicle Biology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
    Fertil Steril 85:1130-41. 2006
    ..To investigate a defined culture condition for the culture of frozen-thawed human ovarian tissue...
  63. ncbi Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation
    Jack Y J Huang
    Department of Obstetrics and Gynecology, McGill University Health Center, McGill University, Montreal, Quebec, Canada
    Fertil Steril 89:567-72. 2008
    ..To report an additional strategy of fertility preservation, which combines ovarian tissue cryobanking with retrieval of immature oocytes from excised ovarian tissue, followed by in vitro maturation (IVM) and vitrification...
  64. ncbi Vitrification by ultra-fast cooling at a low concentration of cryoprotectants in a quartz micro-capillary: a study using murine embryonic stem cells
    Xiaoming He
    Center for Engineering in Medicine and Department of Surgical Service, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, 51 Blossom Street, Boston, MA 02114, USA
    Cryobiology 56:223-32. 2008
    Conventional cryopreservation protocols for slow-freezing or vitrification involve cell injury due to ice formation/cell dehydration or toxicity of high cryoprotectant (CPA) concentrations, respectively...
  65. ncbi Retrieval of immature oocytes from unstimulated ovaries followed by in vitro maturation and vitrification: A novel strategy of fertility preservation for breast cancer patients
    Jack Yu Jen Huang
    Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
    Am J Surg 200:177-83. 2010
    ..We report a novel fertility preservation strategy that may be useful for young breast cancer patients who present with time constraints or concerns about the effect of ovarian stimulation...
  66. ncbi Obstetric outcomes following vitrification of in vitro and in vivo matured oocytes
    Ri Cheng Chian
    McGill Reproductive Center, McGill University Health Centre, Montreal, Quebec, Canada
    Fertil Steril 91:2391-8. 2009
    ..To evaluate obstetric outcomes with oocyte vitrification after ovarian stimulation (OS) and in vitro maturation (IVM) of immature oocytes...
  67. ncbi Long-term storage of mouse spermatozoa after evaporative drying
    Ming W Li
    School of Veterinary Medicine, Center for Comparative Medicine, University of California, Davis, CA 95616, USA
    Reproduction 133:919-29. 2007
    ..The possibility that the storage temperature must be less than the glass transition temperature is discussed...
  68. ncbi Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births
    Jacques Donnez
    Universite Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Department of Gynecology, Cliniques Universitaires Saint Luc, Brussels, Belgium
    Ann Med 43:437-50. 2011
    ..fertility in cancer patients and allow them to conceive when they have overcome their disease: embryo cryopreservation, oocyte cryopreservation, and ovarian tissue cryopreservation...
  69. ncbi Culture of cryopreserved ovarian tissue: state of the art in 2008
    Raffaella Fabbri
    Human Reproductive Medicine Unit, S Orsola Malpighi Hospital, University of Bologna, Bologna, Italy
    Fertil Steril 91:1619-29. 2009
    ..Information on strategies for culturing whole ovarian tissue and isolated follicles are provided as well as an updated and comprehensive view of the role that growth factors have in mediating and regulating in vitro folliculogenesis...
  70. ncbi Children born after cryopreservation of embryos or oocytes: a systematic review of outcome data
    U B Wennerholm
    Perinatal Center, Department of Obstetrics and Gynecology, Institute for Clinical Sciences, Sahlgrenska Academy, SE 416 85 East Hospital, Goteborg, Sweden
    Hum Reprod 24:2158-72. 2009
    ..We reviewed the data in order to evaluate current knowledge of medical outcome for IVF/ICSI children born after cryopreservation, slow freezing and vitrification of early cleavage stage embryos, blastocysts and oocytes.
  71. ncbi Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients
    Dror Meirow
    IVF Unit, Division of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel Hashomer, Israel
    Hum Reprod 23:1007-13. 2008
    ..Methods to exclude presence of cancer cells were used to improve the safety of cryopreservation-reimplantation procedures.
  72. ncbi Assessment of the genotoxicity of three cryoprotectants used for human oocyte vitrification: dimethyl sulfoxide, ethylene glycol and propylene glycol
    M Aye
    Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Fédération de Recherche CNRS 3098 ECCOREV, Universite de la Mediterranee, Facultés de Médecine et Pharmacie, 27 Bd Jean Moulin, 13005 Marseille, France
    Food Chem Toxicol 48:1905-12. 2010
    ..PrOH produced in vitro DNA-damage leading to chromosome mutations in the presence and absence of the S9 Mix. These results showed that high concentrations of EG and PrOH could induce in vitro chromosomal damage in eukaryotic cells...
  73. ncbi Malignant cells are not found in ovarian cortex from breast cancer patients undergoing ovarian cortex cryopreservation
    María Sánchez-Serrano
    Department of Obstetrics and Gynaecology, Hospital Universitario Dr Peset, Av Gaspar Aguilar 90, 46017 Valencia, Spain
    Hum Reprod 24:2238-43. 2009
    Breast cancer is a frequent indication for ovarian cortex cryopreservation due to its high incidence...
  74. ncbi Sperm harvesting and cryopreservation during vasectomy reversal is not cost effective
    Karen E Boyle
    Department of Urology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Fertil Steril 85:961-4. 2006
    OBJECTIVE: To determine whether sperm harvesting and cryopreservation at the time of vasectomy reversal is cost-effective. DESIGN: Model of actual costs and results at five institutions...
  75. ncbi 1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology
    M G Larman
    Colorado Center for Reproductive Medicine, Englewood, CO 80133, USA
    Hum Reprod 22:250-9. 2007
    The aim of this work was to examine the effect of 1,2-propanediol (PrOH) and type of cryopreservation procedure (slow freezing and vitrification) on oocyte physiology.
  76. ncbi In vivo fertility of bull semen following cryopreservation with an LDL (low density lipoprotein) extender: preliminary results of artificial inseminations
    L Amirat-Briand
    Laboratoire de Biotechnologie et pathologie de la reproduction, ONIRIS Ecole Nationale Vétérinaire, Agroalimentaire et de l alimentation Nantes Atlantique, BP 40706, 44307 Nantes, France
    Anim Reprod Sci 122:282-7. 2010
    ..LDL) has been used instead of a standard extender that is already available on the market for the cryopreservation of bovine semen...
  77. ncbi Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome
    Mandy G Katz-Jaffe
    Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA
    Fertil Steril 89:1441-7. 2008
    ..To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome...
  78. ncbi Successful cryopreservation of mouse oocytes by using low concentrations of trehalose and dimethylsulfoxide
    Ali Eroglu
    Institute of Molecular Medicine and Genetics, Department of Medicine, Medical College of Georgia, Augusta, Georgia 30912, USA
    Biol Reprod 80:70-8. 2009
    ..The objective of this study was to examine the potential application of sugars to mammalian oocyte cryopreservation. To this end, we used trehalose, a nonreducing disaccharide, and mouse metaphase II oocytes as models...
  79. ncbi Effects of cryopreservation on human sperm deoxyribonucleic acid integrity
    Nassira Zribi
    Laboratory of Histology Embryology, Medicine Faculty of Sfax, Sfax, Tunisia
    Fertil Steril 93:159-66. 2010
    To evaluate the effect of cryopreservation on sperm motility and viability and to assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation before and after cryopreservation in liquid nitrogen.
  80. ncbi The efficacy and safety of human oocyte cryopreservation by slow cooling
    Andrea Borini
    Tecnobios Procreazione, Bologna, Italy
    Semin Reprod Med 27:443-9. 2009
    ..In the context of human in vitro fertilization (IVF), for decades cryopreservation has been used to store different materials of reproductive function, mainly embryos at various developmental ..
  81. ncbi Ovarian cryopreservation and transplantation for fertility preservation for medical indications: report of an ongoing experience
    Kutluk Oktay
    Department of Obstetrics and Gynecology, New York Medical College, Valhalla, New York and Institute for Fertility Preservation, Center for Human Reproduction, New York, New York 10021, USA
    Fertil Steril 93:762-8. 2010
    ..To assess the indications, safety, utilization, and success of ovarian tissue freezing and transplantation...
  82. ncbi Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols
    B Demirci
    Laboratoire de Zootechnie, Ecole Nationale Veterinaire de Lyon, Marcy l Etoile, France
    Fertil Steril 75:754-62. 2001
    ..To test the toxicity of cryoprotectant in sheep ovarian tissue and to determine optimal conditions for freezing hemiovary cortex...
  83. ncbi Current perspective on primordial follicle cryopreservation and culture for reproductive medicine
    Roger G Gosden
    Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
    Hum Reprod Update 8:105-10. 2002
    ..So far, there has been more success with cryopreservation of primordial follicles than with culture, and frozen-thawed ovarian tissue grafts have restored fertility to ..
  84. ncbi A rational approach to oocyte cryopreservation
    S J Paynter
    Department of Obstetrics and Gynaecology, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Reprod Biomed Online 10:578-86. 2005
    ..Methods of cryopreservation are often poorly described or protocols are not strictly adhered to, resulting in variability of outcome...
  85. ncbi Efficiency of oocyte cryopreservation: a meta-analysis
    Kutluk Oktay
    Fertility Preservation Program at Center for Reproductive Medicine and Infertility, New York, New York, USA
    Fertil Steril 86:70-80. 2006
    To determine the efficiency of oocyte cryopreservation relative to IVF with unfrozen oocytes.
  86. ncbi Effect of cryopreservation on expression of Th1 and Th2 cytokines in blood mononuclear cells from patients with different cytokine profiles, analysed with three common assays: an overall decrease of interleukin-4
    M Kvarnstrom
    Clinical Research Centre, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, Linkoping University, S 581 85 Linkoping, Sweden
    Cryobiology 49:157-68. 2004
    ..Previous studies show that cryopreservation affects cytokine expression, but the findings are not consistent...
  87. ncbi Cryopreservation of adherent human embryonic stem cells
    Lin Ji
    Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA
    Biotechnol Bioeng 88:299-312. 2004
    Standard human embryonic stem (HES) cell cryopreservation methodologies, including slow freezing and vitrification of colonies in suspension, are plagued by poor viability and high differentiation rates upon recovery...
  88. ncbi Parent identity and 'virtual' children: why patients discard rather than donate unused embryos
    Sheryl de Lacey
    Research Centre for Reproductive Health and Repromed, Department of Obstetrics and Gynaecology, The University of Adelaide, South Australia 5005
    Hum Reprod 20:1661-9. 2005
    ..To plan effective counselling practices, it is important to understand the dynamics of decision-making for patients when determining outcomes for unused embryos...
  89. ncbi Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditions
    Jeffrey G Smith
    Vaccine and Biologics Research, Merck Research Laboratories, WP26B 1144, Merck and Co, Inc, 770 Sumneytown Pike, West Point, PA 19486, USA
    Clin Vaccine Immunol 14:527-37. 2007
    ..Although all three AC measures could be effectively applied, the apoptosis AC limit applied was best for separating samples that could respond to antigenic stimulation from samples that could not effectively respond...
  90. ncbi Improved vitrification solutions based on the predictability of vitrification solution toxicity
    Gregory M Fahy
    21st Century Medicine, Inc, 10844 Edison Court, Rancho Cucamonga, CA 91730, USA
    Cryobiology 48:22-35. 2004
    ....
  91. ncbi Cryoloop vitrification of human day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live births
    Nina Desai
    Cleveland Clinic Fertility Centre Cleveland Clinic Foundation, Beachwood, OH 44122, USA
    Reprod Biomed Online 14:208-13. 2007
    Vitrification technology has shown great promise for cryopreservation of human embryos. The majority of this work has been with blastocyst-stage embryos...
  92. ncbi Safety of fertility preservation by ovarian stimulation with letrozole and gonadotropins in patients with breast cancer: a prospective controlled study
    Amr A Azim
    Department of Obstetrics and Gynecology, New York Medical College, Valhalla, New York, USA
    J Clin Oncol 26:2630-5. 2008
    ..determine the effect of controlled ovarian stimulation (COS) using a combination of letrozole with standard fertility medications on disease-free survival in women undergoing embryo or oocyte cryopreservation before adjuvant chemotherapy.
  93. ncbi Biochemical pregnancy after fertilization of an oocyte aspirated from a heterotopic autotransplant of cryopreserved ovarian tissue: case report
    M Rosendahl
    The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Denmark
    Hum Reprod 21:2006-9. 2006
    ..The oocytes of aspirated mature follicles are capable of fertilization after ICSI, and the resulting embryo is competent of producing hCG at detectable levels...
  94. ncbi Human oocyte cryopreservation: new perspectives regarding oocyte survival
    R Fabbri
    IVF Center, Human Reproductive Medicine Unit, Institute of Obstetrics and Gynecology, University of Bologna, 40138 Bologna, Italy
    Hum Reprod 16:411-6. 2001
    The success of human oocyte cryopreservation depends on morphological and biophysical factors that could influence oocyte survival after thawing. Various attempts to cryopreserve human oocytes have been performed with contrasting results...
  95. ncbi Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomalies
    N Noyes
    Department of Obstetrics and Gynecology, NYU Fertility Center, NYU School of Medicine, New York 10016, USA
    Reprod Biomed Online 18:769-76. 2009
    Over the past decade, the number of reported live births resulting from oocyte cryopreservation has rapidly increased. To appreciate the true number of children born, verified live births were tabulated and assessed...
  96. ncbi Human ovarian tissue cryopreservation: indications and feasibility
    Catherine Poirot
    Laboratoire de Biologie de la Reproduction, Groupe Hospitalier Pitie Salpetriere, Universite Paris VI, 75013 Paris, France
    Hum Reprod 17:1447-52. 2002
    The cryopreservation of ovarian tissue may enable women exposed to gonadotoxic treatments to have children at a later date.
  97. ncbi Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa
    Uwe Paasch
    Department of Dermatology Andrology Unit, University of Leipzig, D 04103 Leipzig, Germany
    Biol Reprod 71:1828-37. 2004
    We investigated the impact of cryopreservation and thawing on levels of caspases-3, -8, and -9 activity, intact mitochondrial membrane potential (Deltapsim), and DNA fragmentation in human spermatozoa...
  98. ncbi Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis
    Boon Chin Heng
    Stem Cell Laboratory, Faculty of Dentistry, National University of Singapore, Singapore
    J Biomed Sci 13:433-45. 2006
    ..embryonic stem (hES) cells in clinical therapy and basic scientific research is the development of efficient cryopreservation protocols. Conventional slow-cooling protocols utilizing standard cryoprotectant concentrations i.e...
  99. ncbi Cryopreserved peripheral blood mononuclear cells are suitable for the assessment of immunological markers in type 1 diabetic children
    Stina Axelsson
    Division of Paediatrics and Diabetes Research Centre, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, S 581 85 Linkoping, Sweden
    Cryobiology 57:201-8. 2008
    ..This study investigated the effect of cryopreservation on cytokine and chemokine secretion, and on expression of regulatory T-cell associated markers, in samples ..
  100. ncbi Ovarian function and spontaneous pregnancy after combined heterotopic and orthotopic cryopreserved ovarian tissue transplantation in a patient previously treated with bone marrow transplantation: case report
    Isabelle Demeestere
    Research Laboratory on Human Reproduction, French Speaking Free University of Brussels, Erasme Hospital, Belgium
    Hum Reprod 21:2010-4. 2006
    b>Cryopreservation of ovarian tissue has been proposed for storing gametes of young patients at high risk of premature ovarian failure...
  101. ncbi The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio)
    Shinsuke Seki
    Laboratory of Animal Science, College of Agriculture, Kochi University, Nankoku, Kochi 783 8502, Japan
    Cryobiology 54:121-4. 2007
    To identify a stage feasible for the cryopreservation of zebrafish oocytes, we investigated the permeability to water and cryoprotectants of immature (stage III) and mature (stage V) oocytes...

Research Grants85

  1. CYROBIOLOGICAL PRESERVATION OF ANOPHELES EMBRYOS
    Peter Mazur; Fiscal Year: 2001
    ..That impediment would largely disappear if cryopreservation of embryos or larvae were possible as it is with Drosophila embryos...
  2. ICE-FREE PRESERVATION OF OVARIES FOR LONG-TERM FECUNDITY
    Ying Song; Fiscal Year: 2004
    ..S. alone and thousands lose their ovarian function due to gynecologic surgery. Cryopreservation of ovarian tissue prior to initiation of cancer therapy, followed by autologous transplantation or in vitro ..
  3. Lyophilization of Plasmodium falciparum sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2007
    ..a separate Phase II SBIR, we are developing the prototype, a PfSPZ vaccine using the NF54 strain of Pf using cryopreservation as the method of storage of the live attenuated sporozoites...
  4. A Novel Testicular Germ Cell Maturation Model for Fertility Preservation in Pre-p
    Kirk C Lo; Fiscal Year: 2010
    ..fertility in young men about to undergo cancer therapy are to reduce the toxicity of the cancer therapies and cryopreservation of gametes for future assisted reproductive technology...
  5. HEPTOCELLULAR RESPONCE TO CRYOPRESERVATION
    Mehmet Toner; Fiscal Year: 2002
    ..Although there has been reasonable progress in cryopreservation of cultured hepatocytes, the freezing of isolated hepatocytes is still unreliable...
  6. ORGANIZATION AND FUNCTIONS OF LIPID RAFTS IN SPERMATOZOA
    Alexander Travis; Fiscal Year: 2007
    ..relevance of answering these questions include improving diagnostics for male infertility, improving sperm cryopreservation and handling in vitro, and helping design male contraceptives...
  7. PROTOCOLS FOR MATURING AND FREEZING OOCYTES
    Catherine VandeVoort; Fiscal Year: 2001
    The objective of this project is to devise technical protocols for in vitro maturation (IVM) and cryopreservation of non-human primate oocytes using rhesus monkeys...
  8. PRESERVATION OF GENETICS LINES IN A NON-MAMMALIAN SYSTEM
    Mary Hagedorn; Fiscal Year: 2001
    This proposal addresses cryobiological mechanisms that will assist in developing effective cryopreservation procedures for the germ plasm of teleost fish...
  9. Letrozole Therapy for IVF/ET: Effects on Multiple Pregnancy and Treatment Burden
    Robert Brzyski; Fiscal Year: 2007
    ..with assisted reproductive technology (ART), to reduce the incidence of excess embryo production and embryo cryopreservation associated with ART, and to reduce the burden on patients of ART therapy...
  10. Potency of cryopreserved, irradiated sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2005
    ..Additionally, there have been no advances in methods of cryopreservation for sporozoites in over 20 years...
  11. MOLECULAR ICE CONTROL FOR ENGINEERED TISSUES
    Michael Taylor; Fiscal Year: 2003
    Long term preservation is crucial as an enabling technology for tissue engineering. Cryopreservation would provide an ideal solution to this need except that ice crystallization in complex multicomponent tissues causes injury to the ..
  12. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010
    ..This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro ..
  13. UCD MUTANT MOUSE REGIONAL RESOURCE CENTER
    KC LLOYD; Fiscal Year: 2006
    ..knockouts, and all other kinds of mutant mouse lines at no cost to the donor, and after SPF-rederivation and cryopreservation, distributes breeding stock orgermplasm of genetically, and in many cases phenotypically verified mice for a ..
  14. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart Meyers; Fiscal Year: 2007
    ..This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro ..
  15. Heptocellular Response to Cryopreservation
    Mehmet Toner; Fiscal Year: 2009
    ..desiccation at ambient temperatures, the only existing strategy for long-term storage of mammalian cells is cryopreservation. Cryopreservation relies on cryogenic temperatures (typically < -80fC) in order to halt all chemical ..
  16. ISLET-CELL RESOURCES CTR OF SOUTHERN CALIFORNIA
    ARTHUR RIGGS; Fiscal Year: 2006
    ..first year and more than 200 pancreata by year 4; 2) Technologic advancement of islet-cell proliferation and cryopreservation techniques to allow for multiple donor use or repeated islet transfer from stored or proliferated cells; 3) ..
  17. Light Scattering & Flourescence Study of Sugar Solutions as Cryoprotective Agents
    DAVID LEE SIDEBOTTOM; Fiscal Year: 2010
    Aqueous sugar solutions feature prominently in the cryopreservation and drying of biological membranes and soluable proteins as well as in the anhydrobiosis of many organisms in nature...