cryopreservation

Summary

Summary: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.

Top Publications

  1. ncbi American Society of Clinical Oncology recommendations on fertility preservation in cancer patients
    Stephanie J Lee
    Dana Farber Cancer Institute, Boston, MA, USA
    J Clin Oncol 24:2917-31. 2006
  2. ncbi Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation
    S P Bruder
    Osiris Therapeutics, Inc, Baltimore, MD 21231 2001, USA
    J Cell Biochem 64:278-94. 1997
  3. ncbi Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method
    Masashige Kuwayama
    Kato Ladies Clinic, 7 20 3 Nishishinjuku, Shinjuku, Tokyo, Japan
    Theriogenology 67:73-80. 2007
  4. ncbi Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment
    Victoria Keros
    Karolinska Institute, Division of Obstetrics and Gynaecology, Department of Clinical Science, Technology and Intervention, Karolinska University Hospital, Stockholm, Sweden
    Hum Reprod 22:1384-95. 2007
  5. ncbi Ovary cryopreservation and transplantation for fertility preservation
    S J Silber
    Infertility Center of St Louis, St Luke s Hospital, St Louis, MO 63017, USA
    Mol Hum Reprod 18:59-67. 2012
  6. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
  7. ncbi Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births
    Jacques Donnez
    Universite Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Department of Gynecology, Cliniques Universitaires Saint Luc, Brussels, Belgium
    Ann Med 43:437-50. 2011
  8. ncbi Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification
    Joseph Saragusty
    Department of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, Alfred Kowalke Strasse 17, Berlin, Germany
    Reproduction 141:1-19. 2011
  9. ncbi Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence
    Amy Jones
    Reproductive Biology Associates, 1150 Lake Hearn Dr, Suite 600, Atlanta, GA 30342, USA
    Hum Reprod 19:1861-6. 2004
  10. ncbi Optimization of the cryopreservation and thawing protocol for human hepatocytes for use in cell transplantation
    Claire Terry
    Institute of Liver Studies, King s College London School of Medicine at King s College Hospital, London SE5 9PJ, United Kingdom
    Liver Transpl 16:229-37. 2010

Detail Information

Publications369 found, 100 shown here

  1. ncbi American Society of Clinical Oncology recommendations on fertility preservation in cancer patients
    Stephanie J Lee
    Dana Farber Cancer Institute, Boston, MA, USA
    J Clin Oncol 24:2917-31. 2006
    ..To develop guidance to practicing oncologists about available fertility preservation methods and related issues in people treated for cancer...
  2. ncbi Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation
    S P Bruder
    Osiris Therapeutics, Inc, Baltimore, MD 21231 2001, USA
    J Cell Biochem 64:278-94. 1997
    ..capacity and the osteogenic potential of purified MSCs during extensive subcultivation and following cryopreservation. Primary cultures of MSCs were established from normal iliac crest bone marrow aspirates, an aliquot was ..
  3. ncbi Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method
    Masashige Kuwayama
    Kato Ladies Clinic, 7 20 3 Nishishinjuku, Shinjuku, Tokyo, Japan
    Theriogenology 67:73-80. 2007
    Vitrification is frequently referred to as a novel technology of cryopreservation in embryology, although some young embryologists were born after its first successful application...
  4. ncbi Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment
    Victoria Keros
    Karolinska Institute, Division of Obstetrics and Gynaecology, Department of Clinical Science, Technology and Intervention, Karolinska University Hospital, Stockholm, Sweden
    Hum Reprod 22:1384-95. 2007
    ..We wanted to find optimal methods for cryopreservation of testicular tissue from pre-pubertal boys, modifying techniques developed for fetal and adult human ..
  5. ncbi Ovary cryopreservation and transplantation for fertility preservation
    S J Silber
    Infertility Center of St Louis, St Luke s Hospital, St Louis, MO 63017, USA
    Mol Hum Reprod 18:59-67. 2012
    The aim of this review is to summarize the state-of-the-art of ovarian transplantation and cryopreservation. This field has progressed over the last half century from simple animal experiments to sophisticated application in humans...
  6. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
    ....
  7. ncbi Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births
    Jacques Donnez
    Universite Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Department of Gynecology, Cliniques Universitaires Saint Luc, Brussels, Belgium
    Ann Med 43:437-50. 2011
    ..fertility in cancer patients and allow them to conceive when they have overcome their disease: embryo cryopreservation, oocyte cryopreservation, and ovarian tissue cryopreservation...
  8. ncbi Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification
    Joseph Saragusty
    Department of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, Alfred Kowalke Strasse 17, Berlin, Germany
    Reproduction 141:1-19. 2011
    Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and ..
  9. ncbi Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence
    Amy Jones
    Reproductive Biology Associates, 1150 Lake Hearn Dr, Suite 600, Atlanta, GA 30342, USA
    Hum Reprod 19:1861-6. 2004
    ....
  10. ncbi Optimization of the cryopreservation and thawing protocol for human hepatocytes for use in cell transplantation
    Claire Terry
    Institute of Liver Studies, King s College London School of Medicine at King s College Hospital, London SE5 9PJ, United Kingdom
    Liver Transpl 16:229-37. 2010
    b>Cryopreservation of human hepatocytes is important for their use in hepatocyte transplantation. On thawing, cryopreserved hepatocytes often have reduced viability and metabolic function in comparison with fresh cells...
  11. ncbi Factors affecting cryopreservation of porcine oocytes
    Tamas Somfai
    National Institute of Livestock and Grassland Science, Ibaraki 305 0901, Japan
    J Reprod Dev 58:17-24. 2012
    Recent improvements in cryopreservation of mammalian eggs enable the long-term preservation of female germ cells in several mammalian species. Nevertheless, cryopreservation of porcine oocytes is still considered as a challenge...
  12. ncbi A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos
    David H Edgar
    Reproductive Services Melbourne IVF, Royal Women s Hospital, Parkville, Victoria 3052, Australia
    Hum Reprod Update 18:536-54. 2012
    Vitrification is now a commonly applied technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology...
  13. ncbi Highly efficient vitrification method for cryopreservation of human oocytes
    Masashige Kuwayama
    Kato Ladies Clinic, Tokyo, Japan
    Reprod Biomed Online 11:300-8. 2005
    ..methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0...
  14. ncbi Propagation of human spermatogonial stem cells in vitro
    Hooman Sadri-Ardekani
    Center for Reproductive Medicine, Department of Obstetrics and Gynaecology, University of Amsterdam, Amsterdam, The Netherlands
    JAMA 302:2127-34. 2009
    ..Cryopreserving testicular tissue before chemotherapy and autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for restoration of fertility...
  15. ncbi The relevance of ice crystal formation for the cryopreservation of tissues and organs
    David E Pegg
    Department of Biology, University of York, YO10 5YW, UK
    Cryobiology 60:S36-44. 2010
    ..contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs...
  16. pmc Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use
    Brandon C Perry
    General BioTechnology, LLC, Indianapolis, Indiana, USA
    Tissue Eng Part C Methods 14:149-56. 2008
    ..Further, the recovery of viable DPSC after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate ..
  17. ncbi Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units
    A Bielanski
    Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa Nepean, ON, Canada K2H 8P9
    Hum Reprod 24:2457-67. 2009
    b>Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies...
  18. ncbi Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification
    Yun Xia Cao
    Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, People s Republic of China
    Fertil Steril 92:1306-11. 2009
    ..To compare the survival, fertilization, early embryonic development, and meiotic spindle assembly and chromosome alignment in frozen-thawed human oocytes after slow-freezing and vitrification...
  19. ncbi Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification
    Gary D Smith
    Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA
    Fertil Steril 94:2088-95. 2010
    To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy.
  20. ncbi Storage of human oocytes in the vapor phase of nitrogen
    Ana Cobo
    IVI Universidad de Valencia, Valencia, Spain
    Fertil Steril 94:1903-7. 2010
    ..To evaluate the effectiveness of long-term vapor-phase nitrogen storage of vitrified human oocytes as a strategy for preventing the risk of cross-contamination due to direct contact with the liquid nitrogen (LN)...
  21. ncbi Developmental competence of in vitro-fertilized porcine oocytes after in vitro maturation and solid surface vitrification: effect of cryopreservation on oocyte antioxidative system and cell cycle stage
    Tamas Somfai
    Genetic Diversity Department, National Institute of Agrobiological Sciences, Kannondai 2 1 2, Tsukuba, Ibaraki, Japan
    Cryobiology 55:115-26. 2007
    ..These factors might have contributed to the poor developmental competence of vitrified oocytes...
  22. ncbi Membrane permeability modeling: Kedem-Katchalsky vs a two-parameter formalism
    F W Kleinhans
    Department of Physics, IUPUI, Indianapolis, Indiana, 46202, USA
    Cryobiology 37:271-89. 1998
    ..We also discuss the phenomenological nature of transport parameters in many experiments, especially when both bilayer and channel transport are present...
  23. ncbi Fundamental cryobiology of reproductive cells and tissues
    Erik J Woods
    General BioTechnology, LLC, Harlan Biotechnology Center, 1102 Stadium Drive, Indianapolis, IN 46202, USA
    Cryobiology 48:146-56. 2004
    ..Therefore, there is an urgent need for the development of optimum cryopreservation methods for reproductive cells and tissues from many species...
  24. ncbi Obstetric outcomes following vitrification of in vitro and in vivo matured oocytes
    Ri Cheng Chian
    McGill Reproductive Center, McGill University Health Centre, Montreal, Quebec, Canada
    Fertil Steril 91:2391-8. 2009
    ..To evaluate obstetric outcomes with oocyte vitrification after ovarian stimulation (OS) and in vitro maturation (IVM) of immature oocytes...
  25. ncbi Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue
    Claus Yding Andersen
    Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
    Hum Reprod 23:2266-72. 2008
    b>Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease...
  26. ncbi Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis
    L K Thomson
    Fertility First, PO Box 807, Hurstville, NSW 2220, Australia
    Hum Reprod 24:2061-70. 2009
    Whereas studies have revealed that the cryopreservation of human semen increases sperm DNA fragmentation, the mechanisms involved in this type of cryo-injury are largely unknown...
  27. pmc Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials
    Marta Bull
    Children s Hospital and Regional Medical Center, Seattle, WA, USA
    J Immunol Methods 322:57-69. 2007
    ..function of the resulting cells, including anticoagulant type, time from venipuncture to PBMC isolation/cryopreservation, method of PBMC isolation and procedure for sample shipping...
  28. pmc Conserving, distributing and managing genetically modified mouse lines by sperm cryopreservation
    G Charles Ostermeier
    Technology Evaluation and Development, The Jackson Laboratory, Bar Harbor, Maine, United States of America
    PLoS ONE 3:e2792. 2008
    ..Sperm from C57BL/6 mice are difficult to cryopreserve and recover. Yet, the majority of genetically modified (GM) lines are maintained on this genetic background...
  29. ncbi Outcomes of vitrified early cleavage-stage and blastocyst-stage embryos in a cryopreservation program: evaluation of 3,150 warming cycles
    Ana Cobo
    IVI Valencia, Institut Universitari IVI, Valencia, Spain
    Fertil Steril 98:1138-46.e1. 2012
    ....
  30. ncbi Development of a cryopreservation protocol for type A spermatogonia
    Fariborz Izadyar
    Department of Endocrinology, Faculty of Biology, University Medical Center, Utrecht, The Netherlands
    J Androl 23:537-45. 2002
    The aim of this study was to develop a cryopreservation protocol for type A spermatogonia...
  31. ncbi Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology
    Jaime A Teixeira da Silva
    Faculty of Agriculture, Kagawa University, Miki cho, Ikenobe 2393, Kagawa 761 0795, Japan
    Biotechnol Adv 21:715-66. 2003
    ..This review highlights all available literature that exists on ornamental Chrysanthemum in vitro cell, tissue and organ culture, micropropagation and transformation...
  32. ncbi Effects of cryopreservation on lymphocyte immunophenotype and function
    A Costantini
    Service of Clinical Immunology, Department of Internal Medicine, Ancona University Medical School, Ancona, Italy
    J Immunol Methods 278:145-55. 2003
    b>Cryopreservation of isolated peripheral blood mononuclear cells (PBMCs) for phenotypic and functional analyses is considered a standard procedure in order to minimize operator-dependent inter-assay variability and to optimize the use of ..
  33. ncbi The effect of cancer treatment on female fertility and strategies for preserving fertility
    Theodoros Maltaris
    Department of Obstetrics and Gynecology, Johannes Gutenberg University, Mainz, Germany
    Eur J Obstet Gynecol Reprod Biol 130:148-55. 2007
    ..GnRH analog treatment can preserve fertility in some patients, but not in all. At present, cryopreservation of ovarian tissue appears as a very promising method of providing the cancer patient with a realistic chance ..
  34. pmc Fresh or frozen? Classifying 'spare' embryos for donation to human embryonic stem cell research
    Kathryn Ehrich
    King s College London, UK
    Soc Sci Med 71:2204-11. 2010
    ..reported on here, which received this funding, most of the embryos used for hESC research came from long term cryopreservation storage and/or outside clinics...
  35. ncbi Production of live piglets following cryopreservation of embryos derived from in vitro-matured oocytes
    Hiroshi Nagashima
    Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University, Tama, Kawasaki 214 8571, Japan
    Biol Reprod 76:900-5. 2007
    We have successfully produced healthy piglets following cryopreservation of embryos derived from oocytes matured and fertilized in vitro...
  36. ncbi Effects of osmotic and cold shock on adherent human mesenchymal stem cells during cryopreservation
    Xia Xu
    National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China
    J Biotechnol 162:224-31. 2012
    b>Cryopreservation is one of the most practical methods for the long-term storage of cell-matrix systems to ensure off-shelf availability in tissue engineering, stem cell therapy and drug testing...
  37. ncbi A chronologic review of mature oocyte vitrification research in cattle, pigs, and sheep
    S F Mullen
    The World Egg Bank, Phoenix, Arizona, USA
    Theriogenology 78:1709-19. 2012
    Vitrification as a means of cryopreservation has become a standard approach for oocytes from livestock...
  38. pmc High-throughput cryopreservation of plant cell cultures for functional genomics
    Yoichi Ogawa
    Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
    Plant Cell Physiol 53:943-52. 2012
    ..b>Cryopreservation of these lines may reduce the need for subculturing...
  39. pmc Recent advances in oocyte and ovarian tissue cryopreservation and transplantation
    Kenny A Rodriguez-Wallberg
    Karolinska Institute, Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Best Pract Res Clin Obstet Gynaecol 26:391-405. 2012
    ..In recent years, oocyte cryopreservation methods have further developed, reaching promising standards...
  40. ncbi Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method
    Ana Cobo
    IVI Universidad de Valencia, Valencia, Spain
    Fertil Steril 89:1657-64. 2008
    ..To evaluate the outcome of oocyte vitrification using the Cryotop method, observed in an egg donation program by simultaneously evaluating embryos derived from vitrified and fresh oocytes coming from the same stimulated cycle...
  41. ncbi Twenty years experience with semen cryopreservation in testicular cancer patients: who needs it?
    H Magelssen
    The Norwegian Radium Hospital, Dep of Clinical Research, Montebello, 0310 Oslo, Norway
    Eur Urol 48:779-85. 2005
    To evaluate the role of semen cryopreservation (SCP) in the fertility saving management of testicular cancer (TC) patients, treated at the Norwegian Radium Hospital between 1983 and 2002.
  42. ncbi Vitrification at the pre-antral stage transiently alters inner mitochondrial membrane potential but proteome of in vitro grown and matured mouse oocytes appears unaffected
    Myriam Demant
    Laboratory for Functional Genome Analysis, Gene Center, Ludwig Maximilians Universitat, München 81377, Germany
    Hum Reprod 27:1096-111. 2012
    ..fast and effective method to cryopreserve ovarian tissue, but it might influence mitochondrial activity and affect gene expression to cause persistent alterations in the proteome of oocytes that grow and mature following cryopreservation.
  43. pmc Cornea lenticule viability and structural integrity after refractive lenticule extraction (ReLEx) and cryopreservation
    Karim Mohamed-Noriega
    Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore
    Mol Vis 17:3437-49. 2011
    To assess and compare keratocyte viability and collagen structure in cornea stroma lenticules collected immediately after refractive lenticule extraction (ReLEx) and one month after cryopreservation.
  44. ncbi Cryopreservation and autotransplantation of human ovarian tissue prior to cytotoxic therapy--a technique in its infancy but already successful in fertility preservation
    Michael von Wolff
    Department of Gynecological Endocrinology and Reproductive Medicine, University of Bern, Women s Hospital, Inselspital, Effingerstrasse 102, Bern 3010, Switzerland
    Eur J Cancer 45:1547-53. 2009
    ..Several techniques are now available for fertility preservation in these patients. A new promising method is cryopreservation and transplantation of ovarian cortex...
  45. ncbi Options for fertility preservation in prepubertal boys
    Christine Wyns
    Department of Gynecology, Universite Catholique de Louvain, Cliniques Universitaires St Luc, Avenue Hippocrate 10, Brussels, Belgium
    Hum Reprod Update 16:312-28. 2010
    ..For young boys who do not yet produce spermatozoa, cryopreservation of immature testicular tissue (ITT) is an option to preserve their fertility, albeit still experimental...
  46. pmc Maturation, fertilization, and the structure and function of the endoplasmic reticulum in cryopreserved mouse oocytes
    Katie M Lowther
    Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Biol Reprod 81:147-54. 2009
    Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction...
  47. ncbi Cryopreservation of prepubertal mouse testicular tissue by vitrification
    Mara Curaba
    Gynecology Research Unit, Institut de Recherche Expérimentale et Clinique, Universite Catholique de Louvain, Brussels, Belgium
    Fertil Steril 95:1229-34.e1. 2011
    To compare cryopreservation of prepubertal testicular tissue after vitrification (V) and slow-freezing (SF).
  48. ncbi Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence
    Jun Woo Jo
    Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Gyeonggi, South Korea
    Fertil Steril 96:1239-45. 2011
    ..To investigate the effect of antifreeze protein (AFP) supplementation during mouse oocyte vitrification on the survival, fertilization and embryonic development...
  49. ncbi Optimization of a protocol for cryopreservation of mouse spermatozoa using cryotubes
    Ayumi Hasegawa
    RIKEN BioResource Center, Ibaraki 305 0074, Japan
    J Reprod Dev 58:156-61. 2012
    ..new protocol for sperm freezing using cryotubes as the container to increase the accessibility of mouse sperm cryopreservation. Epididymal spermatozoa were collected from mature ICR or C57BL/6J (B6) males and were suspended in 18% ..
  50. ncbi Bovine oocyte vitrification using the Cryotop method: effect of cumulus cells and vitrification protocol on survival and subsequent development
    X L Zhou
    School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
    Cryobiology 61:66-72. 2010
    ..Theoretical analysis of permeability characteristics and tolerance limits could not explain the low developmental competence of vitrified oocytes...
  51. ncbi Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries
    Monica Antinori
    International Associated Research Institute for Human Reproduction Infertility Unit Day Hospital, Via Timavo No 2, Rome, Italy
    Reprod Biomed Online 14:72-9. 2007
    Vitrification, an ultra-rapid cooling technique, offers a new perspective in attempts to develop an optimal cryopreservation procedure for human oocytes and embryos...
  52. ncbi Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen
    B Macias Garcia
    Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Avda de Universidad s n, Caceres, Spain
    Anim Reprod Sci 114:193-202. 2009
    ..Single-layer centrifugation (SLC) using Androcoll-E significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI...
  53. ncbi Mouse ovarian follicle cryopreservation using vitrification or slow programmed cooling: assessment of in vitro development, maturation, ultra-structure and meiotic spindle organization
    Nina Desai
    Department of Ob Gyn, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    J Obstet Gynaecol Res 37:1-12. 2011
    ..To compare different outcomes of vitrification and slow freezing of isolated pre-antral follicles and to evaluate different cryo-devices for vitrification of isolated follicles...
  54. ncbi Possible improvements in human ovarian grafting by various host and graft treatments
    Or Friedman
    Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel
    Hum Reprod 27:474-82. 2012
    Anticancer treatment poses a high risk of ovarian failure. In many cases cryopreservation of ovarian tissue is the only option for fertility preservation...
  55. ncbi Cryopreservation: a cold look at technology for fertility preservation
    Roger Gosden
    Fertil Steril 96:264-8. 2011
    To outline the history of cryopreservation technology and its contributions to reproductive medicine, including fertility preservation.
  56. ncbi Autotransplantation of cryopreserved ovarian tissue in 12 women with chemotherapy-induced premature ovarian failure: the Danish experience
    Kirsten Tryde Schmidt
    The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    Fertil Steril 95:695-701. 2011
    ..To describe a cohort of 12 Danish women who received autotransplantation of frozen-thawed cryopreserved ovarian tissue because of premature ovarian failure after cancer treatment...
  57. pmc Efficient generation and cryopreservation of cardiomyocytes derived from human embryonic stem cells
    Chunhui Xu
    Geron Corporation, Menlo Park, CA 94025, USA
    Regen Med 6:53-66. 2011
    ..We also aim to examine whether these methods are scalable and whether the differentiated cells can be cryopreserved...
  58. ncbi Infertility patients' beliefs about their embryos and their disposition preferences
    V Provoost
    Bioethics Institute Ghent, Ghent University, Blandijnberg 2 B 9000 Ghent, Belgium
    Hum Reprod 24:896-905. 2009
    ..The objective of this study was to describe how patients speak about their embryos, in moral (e.g. status) and non-moral terms, and to investigate how patients' narratives are related to their disposition preferences...
  59. ncbi Micro-organ ovarian transplantation enables pregnancy: a case report
    Ariel Revel
    Department of Obstetrics and Gynecology, Hadassah Medical Center and Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Hum Reprod 26:1097-103. 2011
    ..We report that preparation of autologous tiny ovarian fragments (MO)s and reimplantation into our patient resulted in IVF pregnancy and delivery of a healthy baby...
  60. ncbi Xenotransplantation of cryopreserved ovarian tissue from patients with ovarian tumors into SCID mice--no evidence of malignant cell contamination
    Laura Lotz
    Department of Obstetrics and Gynecology, Erlangen University Hospital, Erlangen, Germany
    Fertil Steril 95:2612-4.e1. 2011
    ..None of the mice presented symptoms of reintroduced malignancy nor did microscopic and immunohistochemical evaluation of the grafts raise any suspicion of residual malignant disease...
  61. ncbi Isolation of pre-antral follicles from human ovarian medulla tissue
    Stine Gry Kristensen
    Copenhagen University Hospital Rigshospitalet, Copenhagen University, København Ø, Denmark
    Hum Reprod 26:157-66. 2011
    b>Cryopreservation of ovarian tissue for fertility preservation is based on the ovarian cortex that contains the vast majority of the follicular reserve, while the remaining tissue, the medulla is discarded...
  62. ncbi Cryopreservation of human embryos by vitrification or slow freezing: which one is better?
    Efstratios M Kolibianakis
    First Department of Obstetrics and Gynaecology, Unit for Human Reproduction, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
    Curr Opin Obstet Gynecol 21:270-4. 2009
    To summarize the available evidence from randomized controlled trials comparing vitrification versus slow freezing for cryopreservation of human embryos.
  63. ncbi Effect of cryopreservation on sperm DNA integrity in patients with teratospermia
    Guruprasad Kalthur
    Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India
    Fertil Steril 89:1723-7. 2008
    ..To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing...
  64. ncbi Preservation of fertility in females with haematological malignancy
    Jacques Donnez
    Department of Gynecology, Universite Catholique de Louvain, Brussels, Belgium
    Br J Haematol 154:175-84. 2011
    Several options are currently available for the preservation of fertility in cancer patients: embryo cryopreservation, oocyte cryopreservation or ovarian tissue cryopreservation...
  65. ncbi Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis
    Kalliopi E Loutradi
    Unit for Human Reproduction, 1st Department of Obstetrics and Gynaecology, Papageorgiou General Hospital, Thessaloniki, Greece
    Fertil Steril 90:186-93. 2008
    ..the literature systematically in order to identify prospective comparative trials answering the following question: Is vitrification of human embryos associated with a higher postthawing survival rate as compared with slow freezing?..
  66. ncbi Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos
    Lodovico Parmegiani
    Reproductive Medicine Unit, GynePro Medical Centers, Bologna, Italy
    Fertil Steril 94:1525-8. 2010
    ..To evaluate the efficacy of ultraviolet (UV) irradiation for rapid microbial decontamination of liquid nitrogen (LN2)...
  67. ncbi Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique
    Mikkel Rosendahl
    Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Denmark
    Reprod Biomed Online 22:162-71. 2011
    This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated...
  68. ncbi A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation
    B Balaban
    Assisted Reproduction Unit, American Hospital of Istanbul, Guzelbahce Sokak 20, Nisantasi, Istanbul 34365, Turkey
    Hum Reprod 23:1976-82. 2008
    The aim of this study was to compare two methods of cryopreservation for the cleavage-stage human embryo: slow freezing and vitrification.
  69. ncbi The efficacy and safety of human oocyte vitrification
    Zsolt Peter Nagy
    Reproductive Biology Associates, Atlanta, Georgia 30342, USA
    Semin Reprod Med 27:450-5. 2009
    Vitrification is now a widely applied and highly successful approach for cryopreservation in reproductive biology. Rapidly increasing data prove that it is also a highly efficient technique for low-temperature storage of human oocytes...
  70. ncbi Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle
    R Soleimani
    Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Hum Reprod 25:1458-70. 2010
    Ovarian tissue (OT) cryopreservation and transplantation are options for fertility preservation in young female cancer patients.
  71. ncbi Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM
    P Vanderzwalmen
    IVF Centers Prof Zech, Bregenz, Austria
    Reprod Biomed Online 19:700-7. 2009
    ..In spite of reduced cooling rates due to aseptic vitrification conditions, a three-step exposure to cryoprotectant solutions protects the embryos effectively from cryo-injuries and guaranties high survival rates...
  72. ncbi Live birth after vitrification of in vitro matured human oocytes
    Ri Cheng Chian
    McGill Reproductive Centre, McGill University Health Centre, McGill University, Montreal, Quebec, Canada
    Fertil Steril 91:372-6. 2009
    To report the first healthy live birth from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM) and cryopreservation of the oocytes by vitrification.
  73. ncbi Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification
    Mukesh Kumar Gupta
    Department of Animal Biotechnology, Bio Organ Research Center, Konkuk University, 1 Hwayang dong, Gwangjin gu, Seoul 143 701, South Korea
    Theriogenology 67:238-48. 2007
    b>Cryopreservation of normal, lipid-containing porcine oocytes has had limited practical success...
  74. pmc An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples
    Xiao Zhang
    Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
    J Mol Diagn 10:513-9. 2008
    ..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...
  75. ncbi Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice
    Seiji Kito
    Research Center for Radiation Safety, National Institute of Radiological Sciences, 4 9 1 Anagawa Inage Chiba, 263 8555, Japan
    Comp Med 54:564-70. 2004
    ..The data also indicate that rapid freezing of pronucleate embryos following IVF is suitable for cryopreservation and embryo banking of inbred mice and for the production of genetically modified mice.
  76. ncbi Variation in dimethyl sulfoxide use in stem cell transplantation: a survey of EBMT centres
    P Windrum
    Department of Haematology, Belfast City Hospital, Belfast, Northern Ireland
    Bone Marrow Transplant 36:601-3. 2005
    ..A large-scale prospective study of the strategies for reduction in exposure to DMSO and reduction in toxic effects is required before guidelines in the use of DMSO in stem cell cryopreservation can be promulgated.
  77. ncbi Ultra-structural changes and developmental potential of porcine oocytes following vitrification
    Lian Yu Shi
    College of Veterinary Medicine, Gyeongsang National University, Chinju 660 701, Republic of Korea
    Anim Reprod Sci 100:128-40. 2007
    ..In conclusion, porcine oocytes at MII stage are very sensitive to vitrification with altered microtubule localization and mitochondrial organization thus resulting in impaired fertilization and embryo development...
  78. ncbi Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing
    P Vanderzwalmen
    Schoysman Infertility Management Foundation, AZ Jan Portaels, Gendarmeriestraat 65, 1800 Vilvoorde, Belgium
    Hum Reprod 18:1504-11. 2003
    ..The present study was undertaken to examine the usefulness of both vitrification and assisted hatching (AH) on blastocysts that originate from embryos showing different qualities during their cleavage stage...
  79. ncbi Density and distribution of primordial follicles in single pieces of cortex from 21 patients and in individual pieces of cortex from three entire human ovaries
    K L T Schmidt
    Laboratory of Reproductive Biology, Section 5712, The Fertility Clinic, Section 4071, University Hospital of Copenhagen, Rigshospitalet, Denmark
    Hum Reprod 18:1158-64. 2003
    At the time of cryopreservation of ovarian tissue for fertility preservation a small biopsy of ovarian cortex is usually taken for histological evaluation of the follicular reserve...
  80. ncbi MicroRNA profiling of clear cell renal cell carcinoma by whole-genome small RNA deep sequencing of paired frozen and formalin-fixed, paraffin-embedded tissue specimens
    Lihong Weng
    Department of Pathology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Pathol 222:41-51. 2010
    ..To our knowledge, this is the first study to demonstrate that FFPE specimens can be used reliably for miRNA deep-sequencing analysis, making future large-scale clinical cohort/trial-based studies possible...
  81. pmc Performance of plate-based cytokine flow cytometry with automated data analysis
    Maria A Suni
    BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
    BMC Immunol 4:9. 2003
    ..To increase the throughput of CFC assays, we have optimized methods for stimulating, staining, and acquiring whole blood or PBMC samples in 96-well or 24-well plates...
  82. ncbi Cryopreservation characteristics of adipose-derived stem cells: maintenance of differentiation potential and viability
    Brian C Goh
    Stem Cell Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    J Tissue Eng Regen Med 1:322-4. 2007
    ..Because freshly collected adipose tissue is not always readily available, there will be a need for improved cryopreservation methods to reproducibly maintain ASC viablility and multipotentiality in long-term storage...
  83. ncbi Human oocyte cryopreservation: new perspectives regarding oocyte survival
    R Fabbri
    IVF Center, Human Reproductive Medicine Unit, Institute of Obstetrics and Gynecology, University of Bologna, 40138 Bologna, Italy
    Hum Reprod 16:411-6. 2001
    The success of human oocyte cryopreservation depends on morphological and biophysical factors that could influence oocyte survival after thawing. Various attempts to cryopreserve human oocytes have been performed with contrasting results...
  84. ncbi Cryopreservation of porcine embryos derived from in vitro-matured oocytes
    Ritsuko Esaki
    Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University, 1 1 1 Higashimita, Tama, Kawasaki 214 8571, Japan
    Biol Reprod 71:432-7. 2004
    This study describes a cryopreservation method for porcine in vitro-produced (IVP) embryos using as a model parthenogenetic embryos derived from in vitro-matured (IVM) oocytes...
  85. ncbi Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis
    Faten F AbdelHafez
    Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, Cleveland, OH, USA
    Reprod Biomed Online 20:209-22. 2010
    Embryo cryopreservation is an important aspect of assisted reproduction. Many methods have been described, but they have been poorly investigated in randomized trials, highlighting the need for a systematic review of the literature...
  86. ncbi Genome-wide, large-scale production of mutant mice by ENU mutagenesis
    M H Hrabe de Angelis
    Institute of Experimental Genetics, GSF Research Center for Environment and Health, Neuherberg, Germany
    Nat Genet 25:444-7. 2000
    ..Our mutant lines are freely accessible to non-commercial users (for information, see http://www.gsf.de/ieg/groups/enu-mouse.html)...
  87. ncbi Cryopreserved dendritic cells for intratumoral immunotherapy do not require re-culture prior to human vaccination
    Justin John
    Department of Oncology, St George s Hospital Medical School, Cranmer Terrace, London SW17 ORE, United Kingdom
    J Immunol Methods 299:37-46. 2005
    ....
  88. ncbi Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients
    Dror Meirow
    IVF Unit, Division of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel Hashomer, Israel
    Hum Reprod 23:1007-13. 2008
    ..Methods to exclude presence of cancer cells were used to improve the safety of cryopreservation-reimplantation procedures.
  89. ncbi Cryopreservation of spermatozoa in alginic acid capsules
    Andreas Herrler
    Department of Anatomy and Reproductive Biology, Medical School, RWTH Aachen, Aachen, Germany
    Fertil Steril 85:208-13. 2006
    ..To develop a method of freezing small amounts of spermatozoa in polymerized alginic acid drops, which can be liquified after thawing for recovery of the spermatozoa...
  90. ncbi Slow cooling of human oocytes: ultrastructural injuries and apoptotic status
    Roberto Gualtieri
    Department of Structural and Functional Biology, University of Naples Federico II, Monte S Angelo, Naples, Italy
    Fertil Steril 91:1023-34. 2009
    ..To identify the damages caused by slow cooling human metaphase II (MII) oocytes comparing the ultrastructure, inner mitochondrial membrane potential (DeltaPsim), and apoptotic status of fresh and cryopreserved oocytes...
  91. ncbi Intratesticular transplantation of testicular cells from leukemic rats causes transmission of leukemia
    K Jahnukainen
    Pediatric Endocrinology Unit, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden
    Cancer Res 61:706-10. 2001
    ..b>Cryopreservation of the donor germ cells caused a 3- to 6-day delay in the terminal phase of leukemia...
  92. pmc Embryo development of fresh 'versus' vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study
    Laura Rienzi
    G E N E R A Centre for Reproductive Medicine, Clinica Valle Giulia, Via De Notaris 2b, Rome, Italy
    Hum Reprod 25:66-73. 2010
    A successful oocyte cryopreservation programme is of utmost importance where a limited number of oocytes can be inseminated per cycle, to overcome legal and ethical issues related to embryo storage, for oocyte donation programmes and for ..
  93. ncbi Machine perfusion versus cold storage for preservation of kidneys from expanded criteria donors after brain death
    Jürgen Treckmann
    Clinic for General, Visceral and Transplantation Surgery, University Hospital Essen, Hufelandstrasse 55, Germany
    Transpl Int 24:548-54. 2011
    ..3% vs. 80.2%, P=0.02). In the present study, MP preservation clearly reduced the risk of DGF and improved 1-year graft survival and function in ECD kidneys. (Current Controlled Trials number: ISRCTN83876362)...
  94. ncbi Freeze-thawing procedures have no influence on the phenotypic and functional development of dendritic cells generated from peripheral blood CD14+ monocytes
    Shin Ichi Hori
    Hematopoietic Stem Cell Transplant Immunotherapy Unit, National Cancer Center Hospital, Tokyo, Japan
    J Immunother 27:27-35. 2004
    Little is known about the potential influence of cryopreservation on the biologic activities of dendritic cells (DCs)...
  95. ncbi The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissue
    Erik Ernst
    Department of Gynecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark
    Hum Reprod 25:1280-1. 2010
    ..of these children were born in Denmark following transport of the ovarian tissue for a period of 5 h prior to cryopreservation. One of these women, who was originally transplanted with six pieces of ovarian cortex, after having ..
  96. ncbi Mechanism and prevention of cold storage-induced human renal tubular cell injury
    A K Salahudeen
    Department of Medicine, University of Mississippi Medical Center, Jackson 39216 4505, USA
    Transplantation 70:1424-31. 2000
    ..The objective was, therefore, to seek direct evidence for the free radical production during the cold storage of human renal tubular cells, and to define the roles of extrinsic and intrinsic antioxidants in cold-induced cell injury...
  97. pmc The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure
    Shinsuke Seki
    Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996 0840, USA
    Cryobiology 59:75-82. 2009
    ..The two contrasting routes to avoiding it are slow equilibrium freezing and vitrification. The cryopreservation of mammalian oocytes by either method continues to be difficult, but there seems a slowly emerging consensus ..
  98. ncbi Management of severe early ovarian hyperstimulation syndrome by re-initiation of GnRH antagonist
    T G Lainas
    Eugonia Iatriki Erevna IVF Unit, 7 Ventiri str, 11528, Athens, Greece
    Reprod Biomed Online 15:408-12. 2007
    ..proceed to embryo transfer, while if severe early OHSS ensues, antagonist administration combined with embryo cryopreservation appear to be associated with prevention of life-threatening OHSS, facilitation of regression of severe OHSS ..
  99. pmc Orthotopic and heterotopic ovarian tissue transplantation
    I Demeestere
    Research Laboratory on Human Reproduction, Medicine Faculty, Universite Libre de Bruxelles ULB, Erasme Hospital, 808 Route de Lennik, 1070 Brussels, Belgium
    Hum Reprod Update 15:649-65. 2009
    ..Here, we review the different factors affecting follicular survival and growth after grafting...
  100. ncbi Clean technique for cryoprotectant-free vitrification of human spermatozoa
    Vladimir Isachenko
    Department of Endocrinology and Reproductive Medicine, University of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    Reprod Biomed Online 10:350-4. 2005
    ....
  101. ncbi Single sperm cryopreservation on cryoloops: an alternative to hamster zona for freezing individual spermatozoa
    Nina N Desai
    Cleveland Clinic Foundation, Cleveland Clinic Fertility Centre, Suite 220 S, Beachwood, OH 44122, USA
    Reprod Biomed Online 9:47-53. 2004
    Methodology enabling cryopreservation of individual spermatozoa in extreme cases of oligozoospermia would tremendously benefit patients...

Research Grants74

  1. CYROBIOLOGICAL PRESERVATION OF ANOPHELES EMBRYOS
    Peter Mazur; Fiscal Year: 2001
    ..That impediment would largely disappear if cryopreservation of embryos or larvae were possible as it is with Drosophila embryos...
  2. ICE-FREE PRESERVATION OF OVARIES FOR LONG-TERM FECUNDITY
    Ying Song; Fiscal Year: 2004
    ..S. alone and thousands lose their ovarian function due to gynecologic surgery. Cryopreservation of ovarian tissue prior to initiation of cancer therapy, followed by autologous transplantation or in vitro ..
  3. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
    ..b>Cryopreservation is an effective means to store biological materials without functional degradation over a prolonged period of ..
  4. Experimental engineering of ovarian grafting to promote angiogenesis for fertilit
    GLENN LEWIS SCHATTMAN; Fiscal Year: 2010
    ..b>Cryopreservation or vitrification of embryos is a standard of care, and oocyte banking is now emerging for single women, but ..
  5. High Efficiency Microfluidic Purification of Stem Cells to Improve Transplants
    James C Sturm; Fiscal Year: 2012
    ..Because erythrocytes increase both the risk of harmful side effects in transplant patients and the cost of cryopreservation, they must be depleted from the harvested HSPC tissues...
  6. Processing and Preservation of Human Umbilical Cord Stem Cells for Cell Therapeut
    Alfred Bahnson; Fiscal Year: 2010
    ..We also propose to confirm the viability and stability of the stem cell phenotype after processing, cryopreservation and thawing (Aim 2)...
  7. Development of ex vivo Expanded Megakaryocyte Progenitors for Platelet Recovery F
    Holger Karsunky; Fiscal Year: 2009
    ..This will include development of cryopreservation/thawing procedures for the long-term storage of MKP cells...
  8. Real-time microviscosity measurement tools for the cell
    Mark A Haidekker; Fiscal Year: 2010
    ..Studies involving membrane lipid rafts - Analysis of the role of cytoplasmic viscosity in the cryopreservation of cells While it is not the goal of this application to actually apply molecular rotors in the above ..
  9. Characterization of male germline stem cells in a rhesus model of infertility
    Kyle E Orwig; Fiscal Year: 2012
    ..homologous donor cells, and the effect of cryopreservation on SSC regenerative potential...
  10. A Novel Testicular Germ Cell Maturation Model for Fertility Preservation in Pre-p
    Kirk C Lo; Fiscal Year: 2012
    ..fertility in young men about to undergo cancer therapy are to reduce the toxicity of the cancer therapies and cryopreservation of gametes for future assisted reproductive technology...
  11. PEROMYSCUS LABORATORY MODELS FOR BIOMEDICAL RESEARCH
    Gabor Szalai; Fiscal Year: 2013
    ..The research aspect of the project is to continue the development of embryo cryopreservation methods and re- derivation of living animals in order to preserve rare mutants and germplasm for future use ..
  12. AMP-activated protein kinase management will improve oocyte cryopreservation
    Daniel Allen Rappolee; Fiscal Year: 2010
    ..An understanding of the health of the oocyte or embryo depends on knowledge of the stress during cryopreservation, handling, thawing, and reintroduction into culture...
  13. Oklahoma Medical Research Foundation Clinical Research Construction
    Stephen M Prescott; Fiscal Year: 2010
    ..Additional aims focus on construction and support of large, centralized freezers and cryopreservation facilities which will support the above investigators, as well as investigators throughout OMRF...
  14. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2011
    ....
  15. Device for Hyperfast Scalable Vitrification of Germplasm in Large Volumes
    IGOR KATKOV; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cryopreservation of germplasm (GP) (sperm, oocytes, embryos, stem cells, ovarian tissues) is essential for preserving the genetic variety of model animals, reproductive health in humans, the animal ..
  16. Improving Primordial Follicle Survival After Transplantation of Cryopreserved Hum
    Kutluk H Oktay; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Ovarian tissue cryopreservation is one of the key approaches to fertility preservation. It does not require ovarian stimulation and can be done in a relatively short time and even in minors...
  17. Non-Enzymatic Cryogenic Isolation of Therapeutic Cells
    MICHAEL JOHN TAYLOR; Fiscal Year: 2012
    ..injury, to affect the separation of different cell types by virtue of a facilitated differential frezing and cryopreservation techniques...
  18. Hypothermic Storage and Cryopreservation of Corneas
    J Baust; Fiscal Year: 2009
    ..This paradigm shift has led to improved processes for the hypothermic storage and cryopreservation of a variety of human cells and tissues...
  19. Mouse Genetic Models for Alcohol Research
    Tamara J Phillips; Fiscal Year: 2013
    ..Aim 4. Partially defray costs for cryopreservation of long term selected lines and congenic strains...
  20. Development of Germplasm Resources for Preservation of Aquatic Models
    TERRENCE ROBERT TIERSCH; Fiscal Year: 2011
    ..The availability of research lines through cryopreservation will profoundly influence the advancement of biomedical research in toxicology, embryology, genetics, drug ..
  21. CONSORTIUM FOR LARGE-SCALE PRODUCTION AND CRYOPRESERVATION KNOCKOUT MICE: AIDS
    MONICA JEAN JUSTICE; Fiscal Year: 2011
    ..Currently we have some understanding of the function of just one third of human genes. If we are to fully understand human health and disease we must expand knowledge of gene function to all of our genes. ..
  22. Light Scattering & Flourescence Study of Sugar Solutions as Cryoprotective Agents
    DAVID LEE SIDEBOTTOM; Fiscal Year: 2010
    Aqueous sugar solutions feature prominently in the cryopreservation and drying of biological membranes and soluable proteins as well as in the anhydrobiosis of many organisms in nature...
  23. Novel Microcapillary Technology for the Cryopreservation of Human Oocytes
    Mehmet Toner; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Cryopreservation plays a pivotal role in the ability to preserve the long term viability, structure and function of biological cells and tissues...
  24. National Gnotobiotic Rodent Resource Center
    Ryan B Sartor; Fiscal Year: 2013
    ..Resource Center (MMRRC), 2) the experience of the applicant team, 3) innovation in embryo transfer and cryopreservation, 4) The option for onsite tissue collection by external investigators, 5) highly trained technical staff to ..
  25. OPTIMIZING SPERMATOGONIAL TRANSPLANTATION FOR FERTILITY PRESERVATION
    Gunapala Shetty; Fiscal Year: 2010
    ..In such cases cryopreservation of spermatogonia and autologous transplantation is the only a potential method for restoring spermatogenesis ..
  26. Alternative human cell-based models of fatty liver disease
    Bentley Cheatham; Fiscal Year: 2010
    ..Additional confounding factors for the use of primary human hepatocytes include poor recovery from cryopreservation, rapid loss of functional characteristics, and a short lifespan in culture...
  27. High Throughput Cryopreservation of In Vivo-Derived Swine Embryos
    Randall S Prather; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The current methods for cryopreservation of swine embryos are time consuming and troublesome...
  28. CONSORTIUM FOR LARGE-SCALE PRODUCTION AND CRYOPRESERVATION OF KNOCKOUT MICE
    FRANCESCO JOHN DEMAYO; Fiscal Year: 2013
    ..Currently we have some understanding of the function of just one third of human genes. If we are to fully understand human health and disease we must expand knowledge of gene function to all of our genes. ..
  29. Fertility Preservation in Prepubertal Boys: An Experimental Approach
    JILL PHILLIPS GINSBERG; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: This protocol will examine the feasibility of offering testicular cryopreservation to prepubertal boys who are at risk for infertility from cancer therapy (a cohort who currently have no ..
  30. IMPROVING MANUFACTURING AND POTENCY OF CRYOPRESERVED MALARIA SPOROZOITE VACCINE
    STEPHEN LEV HOFFMAN; Fiscal Year: 2013
    ..7 to 6-fold less than that of fresh SPZ. All of Sanaria's products rely on stabilization and cryopreservation of aseptic, purified PfSPZ...
  31. ChNKG2D-Targeted Cellular Cancer Therapy: Phase I Clinical Trial
    Michael W Fanger; Fiscal Year: 2013
    ..and multiple doses, identified biomarkers for use in the clinic, demonstrated equivalent function post- cryopreservation, developed protocols for expansion in serum-free media, designed and sequenced the human vector, expanded ..
  32. Enhanced Propagation of Adult Human Renal Epithelial Cells Allowing for Commercia
    Angela J Westover; Fiscal Year: 2011
    ..The overall goal of this proposal is to optimize the protocols necessary for the isolation, propagation, cryopreservation, differentiation, integration and maintenance of human EP cells in a bioartificial renal epithelial cell ..
  33. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2013
    ..Another possibility is that their efficacy depends more on the degree to which they osmotically dehydrate the cells prior to initiating cooling to -1960C, We propose to test that hypothesis in a quantitative manner. ..
  34. Development of a system (devices and protocols) to improve biospecimen preservati
    BRIAN HRUDKA; Fiscal Year: 2012
    ..The practical benefits of a successful project to improve biospecimen cryopreservation and shipping from patient to analytical laboratory will be to: 1...
  35. R01D: An Interdisciplinary Perspective: A Social Science Examination of Oncoferti
    LINDA LEAH EMANUEL; Fiscal Year: 2011
    ..how families facing a child's cancer diagnosis decide whether or not to participate in the ovarian cryopreservation clinical trial and the role that others, such as healthcare practitioners and religious counsel, play in this ..
  36. Feasibility of immunotherapy by ice-free cryopreservation of engineered human all
    Kelvin G M Brockbank; Fiscal Year: 2012
    ..model with ice-free cryopreserved porcine tissue and human responder cells indicates that an ice- free cryopreservation method developed by the Company modifies the recipient's immune reaction...
  37. Improvement of University of Oregon Animal Research Facilities
    RICHARD W LINTON; Fiscal Year: 2010
    ..research, promote safety and welfare of research animals, researchers, and animal care staff, and expand a cryopreservation repository for genome-wide research...
  38. Cord Blood Biomarkers for Engraftment
    KRISTIN PAGE-CHARTRAND; Fiscal Year: 2013
    ..assay in determining CBU potency and determine the relationship between the segment and the product in the cryopreservation bag, and (3) to prospectively validate the CBA by studying units released for UCBT from the CCBB...
  39. Development of an Economic Process to Store and Recover Homozygous Mouse Strains
    MICHAEL VAN WILES; Fiscal Year: 2010
    ..b>Cryopreservation is used to store strains that are not actively being studied and to protect strains if the breeding colony is ..
  40. Natl Phys Coop to Preserve Fertility for Female Cancer Patients
    R Jeffrey Chang; Fiscal Year: 2011
    ..options for women with cancer;and (3) to disseminate the technical knowledge of follicle maturation and cryopreservation to the Allied centers. The collective goal is to ultimately provide new options for fertility preservation...
  41. CANCER CENTER SUPPORT (CORE) GRANT
    Edison Tak Bun Liu; Fiscal Year: 2013
    ..Sciences for cell biology and microinjection services, and g) Reproductive Sciences for rederivation, cryopreservation and reconstitution of mice...
  42. A Mouse Model for Complex Human Diseases
    James Cheverud; Fiscal Year: 2009
    ..new strains will be genetically characterized for 500 microsatellites and 4290 SNPs and archived by embryonic cryopreservation. Strain genotypes and phenotypes will be made available through our web site, the Mouse Phenome Project, and ..
  43. Technology Project: Genetic Tools and Resources for Orofacial Clefting Research
    Stephen A Murray; Fiscal Year: 2013
    ..Second, we will provide a repository for importation, cryopreservation, genetic quality control, and distribution of new and existing mouse models and tool strains important for ..
  44. Minimizing the role of cryoprotectant toxicity for cryopreservation
    Utkan Demirci; Fiscal Year: 2013
    ..5M CPA concentration. We are adapting this new knowledge to the vital needs of cell cryopreservation at the clinic including discarded anonymous human oocytes...
  45. Automated High Throughput Cryopreservation Using Microfluidics
    STEVEN FRANCIS MULLEN; Fiscal Year: 2010
    ..Having improved means for cell and tissue cryopreservation can increase the effectiveness of bioresource centers containing banks of high quality cells and tissues for ..
  46. NEURAL DEVELOPMENT AND GENETICS OF ZEBRAFISH
    William S Reznikoff; Fiscal Year: 2013
    ..the first week the students learn basic techniques that are standard to any zebrafish laboratory: microscopy, cryopreservation, RNA and antisense morpholino injections, cell transplantation, whole-mount RNA in situ hybridizations and ..
  47. Rat sperm cryobanking for genetic resources
    Yuksel Agca; Fiscal Year: 2010
    ..Although rats are important system for modeling human disease, there is no established germplasm cryopreservation protocol which would allow cost-effective management of rat genetic resources...
  48. Rhesus Monkey Oocyte Resources
    Catherine A Vandevoort; Fiscal Year: 2013
    ..Improved technologies for in vitro maturation (IVM) and cryopreservation of non-human primate oocytes would help alleviate this shortage and increase the efficiency of research in ..
  49. NEUROPEPTIDE REGULATION OF ENTEROPANCREATIC FUNCTION
    Michael W Mulholland; Fiscal Year: 2013
    ..7) Production of large volumes of scrapie infected hamster and mouse brains for purification, 8) Providing cryopreservation of the various mouse lines...
  50. Cryopreservation of Human Epidermal Skin Models
    Lia H Campbell; Fiscal Year: 2010
    ..engineered tissues skin equivalents is a significant issue that can be solved by development of effective cryopreservation methods...
  51. Hyperquenching Cryocooler for Biomolecular X-ray Crystallography
    Robert Newman; Fiscal Year: 2012
    ..overall goal for this project is to design a compact cryocooling device for fast, reproducible and reliable cryopreservation of protein and virus crystals for X-ray cryocrystallography...
  52. Functionalizing Non-Useable Cryopreserved Human Hepatocytes into Useable Hepatic
    Randall McClelland; Fiscal Year: 2011
    ..With cryopreservation technology, human hepatocytes are commercially-available on demand, however, about 75% of the world stores of ..
  53. Microencapsulation of oocytes for low-CPA (cryoprotectant) vitrification
    Xiaoming He; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Oocyte cryopreservation is of great importance to the advancement of assisted reproductive medicine, maintenance of animal resources, and livestock management...
  54. Regeneration of meiotic spindle during oocyte vitrification
    Yusuke Marikawa; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cryopreservation of oocytes is becoming crucial for fertility preservation in women...
  55. A NOVEL APPROACH TO GMP PRODUCTION, CRYOPRESERVATION AND DISTRIBUTION OF TOOTH DE
    Erik J Woods; Fiscal Year: 2010
    ..our group has begun to evaluate the ability of these cells and their respective tissues of origin to survive cryopreservation processing...
  56. Developing a device for visualization of large-scale cryopreservation
    Yoed Rabin; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Preservation of tissues and organs at very low temperatures-cryopreservation-appears to be only feasible alternative to providing high quality biomaterial for transplantation medicine...
  57. A Human Stem Cell-Derived Assay for Detection of Toxicants that Promote Obesity
    Kelvin G M Brockbank; Fiscal Year: 2013
    ..In aim 2 optimization experiments for the cryopreservation, storage and thawing of transduced and control adherent preadipocytes on 96 well assay plates will be ..
  58. Biopsy and Freezing of Later-stage Mouse Blastocysts Using the Dracula Pipette
    Edward E Schmidt; Fiscal Year: 2013
    ..and potentially valuable mouse lines are likely lost or abandoned because the cost and/or complexity of cryopreservation are prohibitive. GeneSearch, Inc...
  59. CRYOPRESERVATION OF DROSOPHILA MELANOGASTER EMBRYOS
    PETER STEPONKUS; Fiscal Year: 1993
    ..We have investigated three different strategies: conventional cryopreservation, vitrification, and supercooling...
  60. High Throughput Microrepository for Genetic Materials
    ROBERT C HAUSHALTER; Fiscal Year: 2012
    ..were to "encourage the development of high throughput and scalable technologies for Germplasm processing and cryopreservation" and "support novel 'high risk/high return'preservation technologies that will break new ground"...
  61. KOMP PHASE II MOUSE PRODUCTION AND CRYOPRESERVATION
    KC KENT LLOYD; Fiscal Year: 2011
    ..track record of handling and converting ES cells to mice, quality control, testing and analyses, germplasm cryopreservation and recovery, and rapid expansion of breeding colonies, especially on the C57BL/6N background, fully supports ..
  62. Establishment of a transgenic monkey of Huntingtons's diesease
    Anthony Wing Sang Chan; Fiscal Year: 2013
    ..HD monkeys, (2) Assessment of molecular and cellular characteristics in transgenic HD monkeys, and (3) Cryopreservation of HD monkey's spermatozoa and embryos...
  63. KOMP PHASE II MOUSE PRODUCTION AND CRYOPRESERVATION
    KC KENT LLOYD; Fiscal Year: 2013
    ..track record of handling and converting ES cells to mice, quality control, testing and analyses, germplasm cryopreservation and recovery, and rapid expansion of breeding colonies, especially on the C57BL/6N background, fully supports ..
  64. CORE GRANT FOR VISION RESEARCH
    Linda D Hazlett; Fiscal Year: 2013
    ..of ocular cells for primary culture, subculture and propagation of established cell lines, and cryopreservation of cells...
  65. Lyophilization of Plasmodium falciparum sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2007
    ..a separate Phase II SBIR, we are developing the prototype, a PfSPZ vaccine using the NF54 strain of Pf using cryopreservation as the method of storage of the live attenuated sporozoites...
  66. BASIC AND APPLIED RESEARCH ON SPERM CRYOPRESERVATION
    James Overstreet; Fiscal Year: 1992
    The long range goal of this project is to improve cryopreservation (CP) procedures for human sperm, so that more sperm competent to fertilize eggs will survive frozen storage, and the success rate of artificial insemination will be ..
  67. OXIDATIVE DAMAGE TO CRYOPRESERVED SPERM
    BAYARD STOREY; Fiscal Year: 1992
    ..test donors for HIV has focussed attention on the damage to the sperm cells brought about by the process of cryopreservation. The percentage of motile cells is decreased and there appears to be lowered pregnancy rates in AI programs, ..
  68. A Novel Device Design for Sperm Cryopreservation
    ERIK WOODS; Fiscal Year: 2009
    ..Sperm cryopreservation would be a simpler and substantially less costly approach compared to embryo cryopreservation which is ..
  69. UMBILICAL CORD STEM CELL CRYOPRESERVATION
    John Critser; Fiscal Year: 2000
    ..immunology, auto-and allotransplantation, and gene therapy transplantation) highlights a great concern that cryopreservation and banking of purified and/or expanded progenitor cells is necessary, and a high and stable cell ..
  70. Potency of cryopreserved, irradiated sporozoite vaccine
    Stephen Hoffman; Fiscal Year: 2005
    ..Additionally, there have been no advances in methods of cryopreservation for sporozoites in over 20 years...
  71. MOLECULAR ICE CONTROL FOR ENGINEERED TISSUES
    Michael Taylor; Fiscal Year: 2003
    Long term preservation is crucial as an enabling technology for tissue engineering. Cryopreservation would provide an ideal solution to this need except that ice crystallization in complex multicomponent tissues causes injury to the ..
  72. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010
    ..This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro ..
  73. FUNDAMENTAL CRYOBIOLOGY OF HUMAN SPERMATOZOA
    John Critser; Fiscal Year: 1992
    ..of the fundamental cryobiology of human sperm will permit the development of optimal procedures for cryopreservation. Knowledge of the permeability of a cell to water and to cryoprotectants can be powerful tools in predicting ..
  74. AUTOMATED CLOSED SYSTEM FOR DMSO REMOVAL FROM PERIPHERAL BLOOD STEM CELLS
    ERIK WOODS; Fiscal Year: 2006
    ..Current PBSC cryopreservation requires the use of molar concentrations of the cryoprotectant additive dimethyl sulfoxide (DMSO)...