Genomes and Genes
Summary: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Publications281 found, 100 shown here
- Cryopreservation and autotransplantation of human ovarian tissue prior to cytotoxic therapy--a technique in its infancy but already successful in fertility preservationMichael von Wolff
Department of Gynecological Endocrinology and Reproductive Medicine, University of Bern, Women s Hospital, Inselspital, Effingerstrasse 102, Bern 3010, Switzerland
Eur J Cancer 45:1547-53. 2009..Several techniques are now available for fertility preservation in these patients. A new promising method is cryopreservation and transplantation of ovarian cortex...
- The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissueErik Ernst
Department of Gynecology and Obstetrics, Aarhus University Hospital, Aarhus, Denmark
Hum Reprod 25:1280-1. 2010..of these children were born in Denmark following transport of the ovarian tissue for a period of 5 h prior to cryopreservation. One of these women, who was originally transplanted with six pieces of ovarian cortex, after having ..
- The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedureShinsuke Seki
Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996 0840, USA
Cryobiology 59:75-82. 2009..The two contrasting routes to avoiding it are slow equilibrium freezing and vitrification. The cryopreservation of mammalian oocytes by either method continues to be difficult, but there seems a slowly emerging consensus ..
- Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrificationYun Xia Cao
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, People s Republic of China
Fertil Steril 92:1306-11. 2009..To compare the survival, fertilization, early embryonic development, and meiotic spindle assembly and chromosome alignment in frozen-thawed human oocytes after slow-freezing and vitrification...
- Storage of human oocytes in the vapor phase of nitrogenAna Cobo
IVI Universidad de Valencia, Valencia, Spain
Fertil Steril 94:1903-7. 2010..To evaluate the effectiveness of long-term vapor-phase nitrogen storage of vitrified human oocytes as a strategy for preventing the risk of cross-contamination due to direct contact with the liquid nitrogen (LN)...
- Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrificationGary D Smith
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA
Fertil Steril 94:2088-95. 2010To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy.
- Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissueClaus Yding Andersen
Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark
Hum Reprod 23:2266-72. 2008b>Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease...
- Risk of contamination of germplasm during cryopreservation and cryobanking in IVF unitsA Bielanski
Canadian Food Inspection Agency, Animal Diseases Research Institute, Germplasm Centre of Expertise, Ottawa Nepean, ON, Canada K2H 8P9
Hum Reprod 24:2457-67. 2009b>Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies...
- American Society of Clinical Oncology recommendations on fertility preservation in cancer patientsStephanie J Lee
Dana-Farber Cancer Institute, Boston, MA, USA
J Clin Oncol 24:2917-31. 2006..Sperm and embryo cryopreservation are considered standard practice and are widely available; other available fertility preservation methods ..
- Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosisL K Thomson
Fertility First, PO Box 807, Hurstville, NSW 2220, Australia
Hum Reprod 24:2061-70. 2009Whereas studies have revealed that the cryopreservation of human semen increases sperm DNA fragmentation, the mechanisms involved in this type of cryo-injury are largely unknown...
- Highly efficient vitrification method for cryopreservation of human oocytesMasashige Kuwayama
Kato Ladies Clinic, Tokyo, Japan
Reprod Biomed Online 11:300-8. 2005..methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0...
- Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trialsMarta Bull
Children s Hospital and Regional Medical Center, Seattle, WA, USA
J Immunol Methods 322:57-69. 2007..function of the resulting cells, including anticoagulant type, time from venipuncture to PBMC isolation/cryopreservation, method of PBMC isolation and procedure for sample shipping...
- Xenotransplantation of cryopreserved ovarian tissue from patients with ovarian tumors into SCID mice--no evidence of malignant cell contaminationLaura Lotz
Department of Obstetrics and Gynecology, Erlangen University Hospital, Erlangen, Germany
Fertil Steril 95:2612-4.e1. 2011..None of the mice presented symptoms of reintroduced malignancy nor did microscopic and immunohistochemical evaluation of the grafts raise any suspicion of residual malignant disease...
- Micro-organ ovarian transplantation enables pregnancy: a case reportAriel Revel
Department of Obstetrics and Gynecology, Hadassah Medical Center and Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
Hum Reprod 26:1097-103. 2011..We report that preparation of autologous tiny ovarian fragments (MO)s and reimplantation into our patient resulted in IVF pregnancy and delivery of a healthy baby...
- Isolation of pre-antral follicles from human ovarian medulla tissueStine Gry Kristensen
Copenhagen University Hospital Rigshospitalet, Copenhagen University, København Ø, Denmark
Hum Reprod 26:157-66. 2011b>Cryopreservation of ovarian tissue for fertility preservation is based on the ovarian cortex that contains the vast majority of the follicular reserve, while the remaining tissue, the medulla is discarded...
- Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysisKalliopi E Loutradi
Unit for Human Reproduction, 1st Department of Obstetrics and Gynaecology, Papageorgiou General Hospital, Thessaloniki, Greece
Fertil Steril 90:186-93. 2008..the literature systematically in order to identify prospective comparative trials answering the following question: Is vitrification of human embryos associated with a higher postthawing survival rate as compared with slow freezing?..
- The efficacy and safety of human oocyte vitrificationZsolt Peter Nagy
Reproductive Biology Associates, Atlanta, Georgia 30342, USA
Semin Reprod Med 27:450-5. 2009Vitrification is now a widely applied and highly successful approach for cryopreservation in reproductive biology. Rapidly increasing data prove that it is also a highly efficient technique for low-temperature storage of human oocytes...
- Cryopreservation of ovarian tissue for a decade in Denmark: a view of the techniqueMikkel Rosendahl
Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Denmark
Reprod Biomed Online 22:162-71. 2011This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated...
- Effect of cryopreservation on sperm DNA integrity in patients with teratospermiaGuruprasad Kalthur
Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, India
Fertil Steril 89:1723-7. 2008..To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing...
- Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafeMarie Madeleine Dolmans
Department of Gynecology, GYNE Research Unit, Institut de Recherche Expérimentale et Clinique, Universite Catholique de Louvain, Brussels, Belgium
Blood 116:2908-14. 2010Ovarian tissue cryopreservation is currently proposed to young cancer patients to preserve their fertility before radiochemotherapy. The potential risk is that the tissue might harbor malignant cells that could induce disease recurrence...
- A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formationB Balaban
Assisted Reproduction Unit, American Hospital of Istanbul, Guzelbahce Sokak 20, Nisantasi, Istanbul 34365, Turkey
Hum Reprod 23:1976-82. 2008The aim of this study was to compare two methods of cryopreservation for the cleavage-stage human embryo: slow freezing and vitrification.
- Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryosLodovico Parmegiani
Reproductive Medicine Unit, GynePro Medical Centers, Bologna, Italy
Fertil Steril 94:1525-8. 2010..To evaluate the efficacy of ultraviolet (UV) irradiation for rapid microbial decontamination of liquid nitrogen (LN2)...
- Cryopreservation of human embryos by vitrification or slow freezing: which one is better?Efstratios M Kolibianakis
First Department of Obstetrics and Gynaecology, Unit for Human Reproduction, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
Curr Opin Obstet Gynecol 21:270-4. 2009To summarize the available evidence from randomized controlled trials comparing vitrification versus slow freezing for cryopreservation of human embryos.
- Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVMP Vanderzwalmen
IVF Centers Prof Zech, Bregenz, Austria
Reprod Biomed Online 19:700-7. 2009..In spite of reduced cooling rates due to aseptic vitrification conditions, a three-step exposure to cryoprotectant solutions protects the embryos effectively from cryo-injuries and guaranties high survival rates...
- Xenotransplantation of cryopreserved human ovarian tissue into murine back muscleR Soleimani
Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
Hum Reprod 25:1458-70. 2010Ovarian tissue (OT) cryopreservation and transplantation are options for fertility preservation in young female cancer patients.
- Preservation of fertility in females with haematological malignancyJacques Donnez
Department of Gynecology, Universite Catholique de Louvain, Brussels, Belgium
Br J Haematol 154:175-84. 2011Several options are currently available for the preservation of fertility in cancer patients: embryo cryopreservation, oocyte cryopreservation or ovarian tissue cryopreservation...
- Orthotopic and heterotopic ovarian tissue transplantationI Demeestere
Research Laboratory on Human Reproduction, Medicine Faculty, Universite Libre de Bruxelles ULB, Erasme Hospital, 808 Route de Lennik, 1070 Brussels, Belgium
Hum Reprod Update 15:649-65. 2009..Here, we review the different factors affecting follicular survival and growth after grafting...
- Live birth after vitrification of in vitro matured human oocytesRi Cheng Chian
McGill Reproductive Centre, McGill University Health Centre, McGill University, Montreal, Quebec, Canada
Fertil Steril 91:372-6. 2009To report the first healthy live birth from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM) and cryopreservation of the oocytes by vitrification.
- Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectantsPrudencio B Pedro
Laboratory of Animal Science, College of Agriculture, Kochi University, Japan
J Reprod Dev 51:235-46. 2005..Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development.
- Analysis of oocyte physiology to improve cryopreservation proceduresDavid K Gardner
Colorado Center for Reproductive Medicine, Englewood, CO 80113, USA
Theriogenology 67:64-72. 2007..Differences in membrane permeability and in physiology are two main reasons why successful oocyte cryopreservation has remained elusive...
- Gene expression profiling of formalin-fixed, paraffin-embedded familial breast tumours using the whole genome-DASL assayNic Waddell
The Queensland Institute of Medical Research, Brisbane, Australia
J Pathol 221:452-61. 2010....
- Vitrification of human 8-cell embryos, a modified protocol for better pregnancy ratesGottumukkala Achyuta Rama Raju
Embryology Division, Krishna IVF Clinic, Zillaparishad Junction, Visakhapatnam, Pincode 530002, Andhra Pradesh, India
Reprod Biomed Online 11:434-7. 2005..Ethylene glycol is a good cryoprotectant to preserve human 8-cell embryos because of its low toxicity as shown by the high survival rate, and vitrification is a promising alternate to the conventional slow-freezing method...
- At what age can human oocytes be obtained?Ariel Revel
Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Fertil Steril 92:458-63. 2009..To determine whether oocyte retrieval and in vitro maturation (IVM) is effective in girls undergoing fertility preservation before cancer treatment...
- Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification methodB E Reubinoff
Department of Obstetrics and Gynecology, Goldyn Savad Institute of Gyne Therapy, Hadassah University Hospital, Ein Kerem, Jerusalem 91120, Israel
Hum Reprod 16:2187-94. 2001..The study aim was to determine whether the open pulled straw (OPS) vitrification method, which is highly effective for the cryopreservation of embryos, might be also efficient for human ES cells.
- Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissueVictoria Keros
Department of Clinical Science, Technology and Intervention, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, K 57, Stockholm SE 141 86, Sweden
Hum Reprod 24:1670-83. 2009..To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus ..
- An efficient method for the rapid establishment of Epstein-Barr virus immortalization of human B lymphocytesH-M Oh
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Chonbuk, Korea
Cell Prolif 36:191-7. 2003..We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester-induced EBV stock significantly improved ..
- Dendritic cell immunotherapy for urological cancers using cryopreserved allogeneic tumour lysate-pulsed cells: a phase I/II studyHardev S Pandha
Department of Histopathology, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
BJU Int 94:412-8. 2004..To assess the feasibility, toxicity and immunogenicity of dendritic cell (DC)-based immunotherapy in patients with advanced urological cancers...
- Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMCLisa S Kierstead
Vaccine and Biologics Research, Merck Research Laboratories, West Point and Wayne, PA 19087, USA
AIDS Res Hum Retroviruses 23:86-92. 2007..These studies establish the importance of optimal conditions for PBMC collection and handling to the success of a clinical development program...
- Fertility preservation in women--a practical guide to preservation techniques and therapeutic strategies in breast cancer, Hodgkin's lymphoma and borderline ovarian tumours by the fertility preservation network FertiPROTEKTMichael von Wolff
Department of Gynaecological Endocrinology and Reproductive Medicine, University Women s Hospital, Effingerstrasse 102, 3010 Bern, Switzerland
Arch Gynecol Obstet 284:427-35. 2011..However, balanced practical recommendations concerning all relevant fertility preservation, to support doctors in counselling and treating patients, are still rare...
- Ultra-rapid vitrification of mouse oocytes in low cryoprotectant concentrationsHo Joon Lee
Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Reprod Biomed Online 20:201-8. 2010The ideal cryopreservation protocol would combine the benefits of slow freezing with the benefits of vitrification. This report describes a method for the ultra-rapid vitrification of oocytes using slush nitrogen in quartz capillaries...
- Autotransplantation of cryopreserved ovarian tissue in 12 women with chemotherapy-induced premature ovarian failure: the Danish experienceKirsten Tryde Schmidt
The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
Fertil Steril 95:695-701. 2011..To describe a cohort of 12 Danish women who received autotransplantation of frozen-thawed cryopreserved ovarian tissue because of premature ovarian failure after cancer treatment...
- Fertility preservation for young patients with cancer: who is at risk and what can be offered?W Hamish B Wallace
Department of Child Life and Health, Division of Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh, UK
Lancet Oncol 6:209-18. 2005....
- Motility, acrosome integrity, membrane integrity and oocyte cleavage rate of sperm separated by swim-up or Percoll gradient method from frozen-thawed buffalo semenA Mehmood
Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan
Anim Reprod Sci 111:141-8. 2009..05), while the Percoll gradient method did not. It has been concluded that separation of sperm from frozen-thawed buffalo semen by swim-up method can be more expedient for IVF in buffalo...
- Sterilisation of skin allograft with gamma irradiationP Rooney
National Health Service Blood and Transplant, Tissue Services, Estuary Banks, Liverpool L24 8RB, UK
Burns 34:664-73. 2008..These non-viable grafts can be made available for surgical use when cryopreserved viable grafts are not available or required...
- Fertility preservation: successful transplantation of cryopreserved ovarian tissue in a young patient previously treated for Hodgkin's diseaseIsabelle Demeestere
Research Laboratory on Human Reproduction, Campus Erasme Bat GE CP636, 808 Route de Lennik, 1070, Brussels, Belgium
Oncologist 12:1437-42. 2007b>Cryopreservation of ovarian tissue is now offered as an experimental procedure to preserve the fertility of young patients with a high risk for premature ovarian failure resulting from cancer therapy...
- Alginate encapsulation as a novel strategy for the cryopreservation of neurospheresRita Malpique
Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras, Portugal
Tissue Eng Part C Methods 16:965-77. 2010..Such applications would greatly benefit from the development of efficient cryopreservation protocols that assure the availability of viable and genetically stable stocks of functional neurospheres...
- Current progress in oocyte and embryo cryopreservation by slow freezing and vitrificationJoseph Saragusty
Department of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, Alfred Kowalke Strasse 17, Berlin, Germany
Reproduction 141:1-19. 2011Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and ..
- A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cellsSepideh Mollamohammadi
Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, PO Box 19395 4644, Tehran, Iran
Hum Reprod 24:2468-76. 2009An essential prerequisite for the future widespread application of human induced pluripotent (hiPSCs) and embryonic stem cells (hESCs) is the development of efficient cryopreservation methods to facilitate their storage and transportation.
- Changes in sperm quality and lipid composition during cryopreservation of boar semenA Maldjian
Bio Innovations, Southburn, East Yorkshire, YO25 9ED, UK
Theriogenology 63:411-21. 2005..concerns the eventual exchanges of lipid components between the spermatozoa and the yolk-based diluent during cryopreservation. Two types of yolk have been considered as ingredients in diluents for cryopreservation: yolks with a ..
- Symposium: Update on prediction and management of OHSS. Prevention of OHSSMohamed Aboulghar
Cairo University and the Egyptian IVF Center, Maadi, Cairo, Egypt
Reprod Biomed Online 19:33-42. 2009..Intravenous albumin is useful in the prevention when given at time of oocyte retrieval. Cryopreservation of all embryos will reduce late-onset OHSS but not early-onset OHSS...
- The effect of cryopreservation or heating on the mechanical properties and histomorphology of rat bone-patellar tendon-boneHwang Jung Park
Department of Orthopaedic Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
Cell Tissue Bank 10:11-8. 2009The effects of cryopreservation on tendon allograft have been reported, but remain unclear, particularly the potential effects on mechanical properties and histological changes by ice crystal formation...
- Effect of different cryopreservation protocols on the metaphase II spindle in human oocytesA Cobo
IVI Universidad de Valencia Plaza de la Policía local, 3 46015 Valencia, Spain
Reprod Biomed Online 17:350-9. 2008The aim of this study was to evaluate the impact of different cryopreservation protocols on the repolymerization of metaphase (M)II spindles in human oocytes...
- Ovarian cryopreservation for fertility preservation: indications and outcomesR A Anderson
The Queen s Medical Research Institute, Centre for Reproductive Biology, University of Edinburgh, Edinburgh, UK
Reproduction 136:681-9. 2008..provides significantly different challenges to that for the male, with the only established method being cryopreservation of embryos thus necessitating the involvement of a male...
- Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoaG Martin
Laboratoire de Biologie de la Reproduction GIMAP, Hopital Nord, Saint Etienne, France
Hum Reprod 22:380-8. 2007b>Cryopreservation/thawing of bovine spermatozoa induces a reduction in cell viability and is possibly associated with a form of programmed cell death that we previously named 'apoptosis-like phenomenon'.
- New options in assisted reproduction technology: the Cryotop method of oocyte vitrificationAna Cobo
IVI Universidad de Valencia, Plaza de la Policia Local, 3 46015 Valencia, Spain
Reprod Biomed Online 17:68-72. 2008The Cryotop vitrification method has been shown to be a very useful tool for oocyte cryopreservation, giving excellent results regarding survival and clinical outcome...
- An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samplesXiao Zhang
Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
J Mol Diagn 10:513-9. 2008..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...
- Report of four donor-recipient oocyte cryopreservation cycles resulting in high pregnancy and implantation ratesJason Barritt
Department of Obstetrics and Gynecology, and Department of Reproductive Endocrinology and Infertility, Mount Sinai School of Medicine, New York, New York, USA
Fertil Steril 87:189.e13-7. 2007To determine the clinical potential of donor-oocyte cryopreservation and thaw techniques for recipient patients.
- Clinical-grade ex vivo-expanded human natural killer cells up-regulate activating receptors and death receptor ligands and have enhanced cytolytic activity against tumor cellsMaria Berg
Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Cytotherapy 11:341-55. 2009..We provide a method for expanding highly cytotoxic clinical-grade NK cells in vitro for adoptive transfer following bortezomib treatment in patients with advanced malignancies...
- Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll. Arg.)Ludovic Lardet
CIRAD CP, UMR 1098, TA 80 03, Avenue Agropolis, 34398 Montpellier, France
Plant Cell Rep 26:559-69. 2007A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis...
- Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerioHuiping Yang
Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
Theriogenology 68:128-36. 2007Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible...
- Integrated morphophysiological assessment of two methods for sperm selection in bovine embryo production in vitroA Cesari
Instituto de Investigaciones Biologicas, Facultad de Ciencias Exactas y Naturales, UNMdP CC 1245 7600 Mar del Plata, Buenos Aires, Argentina
Theriogenology 66:1185-93. 2006....
- Morphological and ultrastructural evaluation of cultured frozen-thawed human fetal ovarian tissueJean Clair Sadeu
Follicle Biology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
Fertil Steril 85:1130-41. 2006..To investigate a defined culture condition for the culture of frozen-thawed human ovarian tissue...
- Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservationJack Y J Huang
Department of Obstetrics and Gynecology, McGill University Health Center, McGill University, Montreal, Quebec, Canada
Fertil Steril 89:567-72. 2008..To report an additional strategy of fertility preservation, which combines ovarian tissue cryobanking with retrieval of immature oocytes from excised ovarian tissue, followed by in vitro maturation (IVM) and vitrification...
- Vitrification by ultra-fast cooling at a low concentration of cryoprotectants in a quartz micro-capillary: a study using murine embryonic stem cellsXiaoming He
Center for Engineering in Medicine and Department of Surgical Service, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, 51 Blossom Street, Boston, MA 02114, USA
Cryobiology 56:223-32. 2008Conventional cryopreservation protocols for slow-freezing or vitrification involve cell injury due to ice formation/cell dehydration or toxicity of high cryoprotectant (CPA) concentrations, respectively...
- Retrieval of immature oocytes from unstimulated ovaries followed by in vitro maturation and vitrification: A novel strategy of fertility preservation for breast cancer patientsJack Yu Jen Huang
Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
Am J Surg 200:177-83. 2010..We report a novel fertility preservation strategy that may be useful for young breast cancer patients who present with time constraints or concerns about the effect of ovarian stimulation...
- Obstetric outcomes following vitrification of in vitro and in vivo matured oocytesRi Cheng Chian
McGill Reproductive Center, McGill University Health Centre, Montreal, Quebec, Canada
Fertil Steril 91:2391-8. 2009..To evaluate obstetric outcomes with oocyte vitrification after ovarian stimulation (OS) and in vitro maturation (IVM) of immature oocytes...
- Long-term storage of mouse spermatozoa after evaporative dryingMing W Li
School of Veterinary Medicine, Center for Comparative Medicine, University of California, Davis, CA 95616, USA
Reproduction 133:919-29. 2007..The possibility that the storage temperature must be less than the glass transition temperature is discussed...
- Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live birthsJacques Donnez
Universite Catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Department of Gynecology, Cliniques Universitaires Saint Luc, Brussels, Belgium
Ann Med 43:437-50. 2011..fertility in cancer patients and allow them to conceive when they have overcome their disease: embryo cryopreservation, oocyte cryopreservation, and ovarian tissue cryopreservation...
- Culture of cryopreserved ovarian tissue: state of the art in 2008Raffaella Fabbri
Human Reproductive Medicine Unit, S Orsola Malpighi Hospital, University of Bologna, Bologna, Italy
Fertil Steril 91:1619-29. 2009..Information on strategies for culturing whole ovarian tissue and isolated follicles are provided as well as an updated and comprehensive view of the role that growth factors have in mediating and regulating in vitro folliculogenesis...
- Children born after cryopreservation of embryos or oocytes: a systematic review of outcome dataU B Wennerholm
Perinatal Center, Department of Obstetrics and Gynecology, Institute for Clinical Sciences, Sahlgrenska Academy, SE 416 85 East Hospital, Goteborg, Sweden
Hum Reprod 24:2158-72. 2009..We reviewed the data in order to evaluate current knowledge of medical outcome for IVF/ICSI children born after cryopreservation, slow freezing and vitrification of early cleavage stage embryos, blastocysts and oocytes.
- Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patientsDror Meirow
IVF Unit, Division of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel Hashomer, Israel
Hum Reprod 23:1007-13. 2008..Methods to exclude presence of cancer cells were used to improve the safety of cryopreservation-reimplantation procedures.
- Assessment of the genotoxicity of three cryoprotectants used for human oocyte vitrification: dimethyl sulfoxide, ethylene glycol and propylene glycolM Aye
Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Fédération de Recherche CNRS 3098 ECCOREV, Universite de la Mediterranee, Facultés de Médecine et Pharmacie, 27 Bd Jean Moulin, 13005 Marseille, France
Food Chem Toxicol 48:1905-12. 2010..PrOH produced in vitro DNA-damage leading to chromosome mutations in the presence and absence of the S9 Mix. These results showed that high concentrations of EG and PrOH could induce in vitro chromosomal damage in eukaryotic cells...
- Malignant cells are not found in ovarian cortex from breast cancer patients undergoing ovarian cortex cryopreservationMaría Sánchez-Serrano
Department of Obstetrics and Gynaecology, Hospital Universitario Dr Peset, Av Gaspar Aguilar 90, 46017 Valencia, Spain
Hum Reprod 24:2238-43. 2009Breast cancer is a frequent indication for ovarian cortex cryopreservation due to its high incidence...
- Sperm harvesting and cryopreservation during vasectomy reversal is not cost effectiveKaren E Boyle
Department of Urology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Fertil Steril 85:961-4. 2006OBJECTIVE: To determine whether sperm harvesting and cryopreservation at the time of vasectomy reversal is cost-effective. DESIGN: Model of actual costs and results at five institutions...
- 1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiologyM G Larman
Colorado Center for Reproductive Medicine, Englewood, CO 80133, USA
Hum Reprod 22:250-9. 2007The aim of this work was to examine the effect of 1,2-propanediol (PrOH) and type of cryopreservation procedure (slow freezing and vitrification) on oocyte physiology.
- In vivo fertility of bull semen following cryopreservation with an LDL (low density lipoprotein) extender: preliminary results of artificial inseminationsL Amirat-Briand
Laboratoire de Biotechnologie et pathologie de la reproduction, ONIRIS Ecole Nationale Vétérinaire, Agroalimentaire et de l alimentation Nantes Atlantique, BP 40706, 44307 Nantes, France
Anim Reprod Sci 122:282-7. 2010..LDL) has been used instead of a standard extender that is already available on the market for the cryopreservation of bovine semen...
- Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteomeMandy G Katz-Jaffe
Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA
Fertil Steril 89:1441-7. 2008..To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome...
- Successful cryopreservation of mouse oocytes by using low concentrations of trehalose and dimethylsulfoxideAli Eroglu
Institute of Molecular Medicine and Genetics, Department of Medicine, Medical College of Georgia, Augusta, Georgia 30912, USA
Biol Reprod 80:70-8. 2009..The objective of this study was to examine the potential application of sugars to mammalian oocyte cryopreservation. To this end, we used trehalose, a nonreducing disaccharide, and mouse metaphase II oocytes as models...
- Effects of cryopreservation on human sperm deoxyribonucleic acid integrityNassira Zribi
Laboratory of Histology Embryology, Medicine Faculty of Sfax, Sfax, Tunisia
Fertil Steril 93:159-66. 2010To evaluate the effect of cryopreservation on sperm motility and viability and to assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation before and after cryopreservation in liquid nitrogen.
- The efficacy and safety of human oocyte cryopreservation by slow coolingAndrea Borini
Tecnobios Procreazione, Bologna, Italy
Semin Reprod Med 27:443-9. 2009..In the context of human in vitro fertilization (IVF), for decades cryopreservation has been used to store different materials of reproductive function, mainly embryos at various developmental ..
- Ovarian cryopreservation and transplantation for fertility preservation for medical indications: report of an ongoing experienceKutluk Oktay
Department of Obstetrics and Gynecology, New York Medical College, Valhalla, New York and Institute for Fertility Preservation, Center for Human Reproduction, New York, New York 10021, USA
Fertil Steril 93:762-8. 2010..To assess the indications, safety, utilization, and success of ovarian tissue freezing and transplantation...
- Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocolsB Demirci
Laboratoire de Zootechnie, Ecole Nationale Veterinaire de Lyon, Marcy l Etoile, France
Fertil Steril 75:754-62. 2001..To test the toxicity of cryoprotectant in sheep ovarian tissue and to determine optimal conditions for freezing hemiovary cortex...
- Current perspective on primordial follicle cryopreservation and culture for reproductive medicineRoger G Gosden
Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada
Hum Reprod Update 8:105-10. 2002..So far, there has been more success with cryopreservation of primordial follicles than with culture, and frozen-thawed ovarian tissue grafts have restored fertility to ..
- A rational approach to oocyte cryopreservationS J Paynter
Department of Obstetrics and Gynaecology, Wales College of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Reprod Biomed Online 10:578-86. 2005..Methods of cryopreservation are often poorly described or protocols are not strictly adhered to, resulting in variability of outcome...
- Efficiency of oocyte cryopreservation: a meta-analysisKutluk Oktay
Fertility Preservation Program at Center for Reproductive Medicine and Infertility, New York, New York, USA
Fertil Steril 86:70-80. 2006To determine the efficiency of oocyte cryopreservation relative to IVF with unfrozen oocytes.
- Effect of cryopreservation on expression of Th1 and Th2 cytokines in blood mononuclear cells from patients with different cytokine profiles, analysed with three common assays: an overall decrease of interleukin-4M Kvarnstrom
Clinical Research Centre, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, Linkoping University, S 581 85 Linkoping, Sweden
Cryobiology 49:157-68. 2004..Previous studies show that cryopreservation affects cytokine expression, but the findings are not consistent...
- Cryopreservation of adherent human embryonic stem cellsLin Ji
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA
Biotechnol Bioeng 88:299-312. 2004Standard human embryonic stem (HES) cell cryopreservation methodologies, including slow freezing and vitrification of colonies in suspension, are plagued by poor viability and high differentiation rates upon recovery...
- Parent identity and 'virtual' children: why patients discard rather than donate unused embryosSheryl de Lacey
Research Centre for Reproductive Health and Repromed, Department of Obstetrics and Gynaecology, The University of Adelaide, South Australia 5005
Hum Reprod 20:1661-9. 2005..To plan effective counselling practices, it is important to understand the dynamics of decision-making for patients when determining outcomes for unused embryos...
- Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditionsJeffrey G Smith
Vaccine and Biologics Research, Merck Research Laboratories, WP26B 1144, Merck and Co, Inc, 770 Sumneytown Pike, West Point, PA 19486, USA
Clin Vaccine Immunol 14:527-37. 2007..Although all three AC measures could be effectively applied, the apoptosis AC limit applied was best for separating samples that could respond to antigenic stimulation from samples that could not effectively respond...
- Improved vitrification solutions based on the predictability of vitrification solution toxicityGregory M Fahy
21st Century Medicine, Inc, 10844 Edison Court, Rancho Cucamonga, CA 91730, USA
Cryobiology 48:22-35. 2004....
- Cryoloop vitrification of human day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live birthsNina Desai
Cleveland Clinic Fertility Centre Cleveland Clinic Foundation, Beachwood, OH 44122, USA
Reprod Biomed Online 14:208-13. 2007Vitrification technology has shown great promise for cryopreservation of human embryos. The majority of this work has been with blastocyst-stage embryos...
- Safety of fertility preservation by ovarian stimulation with letrozole and gonadotropins in patients with breast cancer: a prospective controlled studyAmr A Azim
Department of Obstetrics and Gynecology, New York Medical College, Valhalla, New York, USA
J Clin Oncol 26:2630-5. 2008..determine the effect of controlled ovarian stimulation (COS) using a combination of letrozole with standard fertility medications on disease-free survival in women undergoing embryo or oocyte cryopreservation before adjuvant chemotherapy.
- Biochemical pregnancy after fertilization of an oocyte aspirated from a heterotopic autotransplant of cryopreserved ovarian tissue: case reportM Rosendahl
The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Denmark
Hum Reprod 21:2006-9. 2006..The oocytes of aspirated mature follicles are capable of fertilization after ICSI, and the resulting embryo is competent of producing hCG at detectable levels...
- Human oocyte cryopreservation: new perspectives regarding oocyte survivalR Fabbri
IVF Center, Human Reproductive Medicine Unit, Institute of Obstetrics and Gynecology, University of Bologna, 40138 Bologna, Italy
Hum Reprod 16:411-6. 2001The success of human oocyte cryopreservation depends on morphological and biophysical factors that could influence oocyte survival after thawing. Various attempts to cryopreserve human oocytes have been performed with contrasting results...
- Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomaliesN Noyes
Department of Obstetrics and Gynecology, NYU Fertility Center, NYU School of Medicine, New York 10016, USA
Reprod Biomed Online 18:769-76. 2009Over the past decade, the number of reported live births resulting from oocyte cryopreservation has rapidly increased. To appreciate the true number of children born, verified live births were tabulated and assessed...
- Human ovarian tissue cryopreservation: indications and feasibilityCatherine Poirot
Laboratoire de Biologie de la Reproduction, Groupe Hospitalier Pitie Salpetriere, Universite Paris VI, 75013 Paris, France
Hum Reprod 17:1447-52. 2002The cryopreservation of ovarian tissue may enable women exposed to gonadotoxic treatments to have children at a later date.
- Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoaUwe Paasch
Department of Dermatology Andrology Unit, University of Leipzig, D 04103 Leipzig, Germany
Biol Reprod 71:1828-37. 2004We investigated the impact of cryopreservation and thawing on levels of caspases-3, -8, and -9 activity, intact mitochondrial membrane potential (Deltapsim), and DNA fragmentation in human spermatozoa...
- Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosisBoon Chin Heng
Stem Cell Laboratory, Faculty of Dentistry, National University of Singapore, Singapore
J Biomed Sci 13:433-45. 2006..embryonic stem (hES) cells in clinical therapy and basic scientific research is the development of efficient cryopreservation protocols. Conventional slow-cooling protocols utilizing standard cryoprotectant concentrations i.e...
- Cryopreserved peripheral blood mononuclear cells are suitable for the assessment of immunological markers in type 1 diabetic childrenStina Axelsson
Division of Paediatrics and Diabetes Research Centre, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, S 581 85 Linkoping, Sweden
Cryobiology 57:201-8. 2008..This study investigated the effect of cryopreservation on cytokine and chemokine secretion, and on expression of regulatory T-cell associated markers, in samples ..
- Ovarian function and spontaneous pregnancy after combined heterotopic and orthotopic cryopreserved ovarian tissue transplantation in a patient previously treated with bone marrow transplantation: case reportIsabelle Demeestere
Research Laboratory on Human Reproduction, French Speaking Free University of Brussels, Erasme Hospital, Belgium
Hum Reprod 21:2010-4. 2006b>Cryopreservation of ovarian tissue has been proposed for storing gametes of young patients at high risk of premature ovarian failure...
- The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio)Shinsuke Seki
Laboratory of Animal Science, College of Agriculture, Kochi University, Nankoku, Kochi 783 8502, Japan
Cryobiology 54:121-4. 2007To identify a stage feasible for the cryopreservation of zebrafish oocytes, we investigated the permeability to water and cryoprotectants of immature (stage III) and mature (stage V) oocytes...
- CYROBIOLOGICAL PRESERVATION OF ANOPHELES EMBRYOSPeter Mazur; Fiscal Year: 2001..That impediment would largely disappear if cryopreservation of embryos or larvae were possible as it is with Drosophila embryos...
- ICE-FREE PRESERVATION OF OVARIES FOR LONG-TERM FECUNDITYYing Song; Fiscal Year: 2004..S. alone and thousands lose their ovarian function due to gynecologic surgery. Cryopreservation of ovarian tissue prior to initiation of cancer therapy, followed by autologous transplantation or in vitro ..
- Lyophilization of Plasmodium falciparum sporozoite vaccineStephen Hoffman; Fiscal Year: 2007..a separate Phase II SBIR, we are developing the prototype, a PfSPZ vaccine using the NF54 strain of Pf using cryopreservation as the method of storage of the live attenuated sporozoites...
- A Novel Testicular Germ Cell Maturation Model for Fertility Preservation in Pre-pKirk C Lo; Fiscal Year: 2010..fertility in young men about to undergo cancer therapy are to reduce the toxicity of the cancer therapies and cryopreservation of gametes for future assisted reproductive technology...
- HEPTOCELLULAR RESPONCE TO CRYOPRESERVATIONMehmet Toner; Fiscal Year: 2002..Although there has been reasonable progress in cryopreservation of cultured hepatocytes, the freezing of isolated hepatocytes is still unreliable...
- ORGANIZATION AND FUNCTIONS OF LIPID RAFTS IN SPERMATOZOAAlexander Travis; Fiscal Year: 2007..relevance of answering these questions include improving diagnostics for male infertility, improving sperm cryopreservation and handling in vitro, and helping design male contraceptives...
- PROTOCOLS FOR MATURING AND FREEZING OOCYTESCatherine VandeVoort; Fiscal Year: 2001The objective of this project is to devise technical protocols for in vitro maturation (IVM) and cryopreservation of non-human primate oocytes using rhesus monkeys...
- PRESERVATION OF GENETICS LINES IN A NON-MAMMALIAN SYSTEMMary Hagedorn; Fiscal Year: 2001This proposal addresses cryobiological mechanisms that will assist in developing effective cryopreservation procedures for the germ plasm of teleost fish...
- Letrozole Therapy for IVF/ET: Effects on Multiple Pregnancy and Treatment BurdenRobert Brzyski; Fiscal Year: 2007..with assisted reproductive technology (ART), to reduce the incidence of excess embryo production and embryo cryopreservation associated with ART, and to reduce the burden on patients of ART therapy...
- Potency of cryopreserved, irradiated sporozoite vaccineStephen Hoffman; Fiscal Year: 2005..Additionally, there have been no advances in methods of cryopreservation for sporozoites in over 20 years...
- MOLECULAR ICE CONTROL FOR ENGINEERED TISSUESMichael Taylor; Fiscal Year: 2003Long term preservation is crucial as an enabling technology for tissue engineering. Cryopreservation would provide an ideal solution to this need except that ice crystallization in complex multicomponent tissues causes injury to the ..
- Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative StressStuart A Meyers; Fiscal Year: 2010..This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro ..
- UCD MUTANT MOUSE REGIONAL RESOURCE CENTERKC LLOYD; Fiscal Year: 2006..knockouts, and all other kinds of mutant mouse lines at no cost to the donor, and after SPF-rederivation and cryopreservation, distributes breeding stock orgermplasm of genetically, and in many cases phenotypically verified mice for a ..
- Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative StressStuart Meyers; Fiscal Year: 2007..This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro ..
- Heptocellular Response to CryopreservationMehmet Toner; Fiscal Year: 2009..desiccation at ambient temperatures, the only existing strategy for long-term storage of mammalian cells is cryopreservation. Cryopreservation relies on cryogenic temperatures (typically < -80fC) in order to halt all chemical ..
- ISLET-CELL RESOURCES CTR OF SOUTHERN CALIFORNIAARTHUR RIGGS; Fiscal Year: 2006..first year and more than 200 pancreata by year 4; 2) Technologic advancement of islet-cell proliferation and cryopreservation techniques to allow for multiple donor use or repeated islet transfer from stored or proliferated cells; 3) ..
- Light Scattering & Flourescence Study of Sugar Solutions as Cryoprotective AgentsDAVID LEE SIDEBOTTOM; Fiscal Year: 2010Aqueous sugar solutions feature prominently in the cryopreservation and drying of biological membranes and soluable proteins as well as in the anhydrobiosis of many organisms in nature...