biomolecular nuclear magnetic resonance

Summary

Summary: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.

Top Publications

  1. pmc BioMagResBank
    Eldon L Ulrich
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Nucleic Acids Res 36:D402-8. 2008
  2. ncbi Automated NMR structure calculation with CYANA
    Peter Güntert
    RIKEN Genomic Sciences Center, Yokohama, Japan
    Methods Mol Biol 278:353-78. 2004
  3. ncbi The Xplor-NIH NMR molecular structure determination package
    Charles D Schwieters
    Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Building 12A, 20892 5624, Bethesda, MD, USA
    J Magn Reson 160:65-73. 2003
  4. ncbi Targeted profiling: quantitative analysis of 1H NMR metabolomics data
    Aalim M Weljie
    Chenomx Inc, Edmonton, Alberta, Canada, and Metabolomics Research Centre, University of Calgary, Calgary, Canada
    Anal Chem 78:4430-42. 2006
  5. pmc Not so crystal clear: the structure of the human telomere G-quadruplex in solution differs from that present in a crystal
    Jing Li
    Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216 4505, USA
    Nucleic Acids Res 33:4649-59. 2005
  6. ncbi Evaluating protein structures determined by structural genomics consortia
    Aneerban Bhattacharya
    Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers University and Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Proteins 66:778-95. 2007
  7. pmc HMDB: the Human Metabolome Database
    David S Wishart
    Department of Computing Science, University of Alberta, Edmonton, AB, Canada T6G 2E8
    Nucleic Acids Res 35:D521-6. 2007
  8. pmc An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human BCL-2 promoter region in solution
    Jixun Dai
    College of Pharmacy, University of Arizona, Tucson, 85721, USA
    J Am Chem Soc 128:1096-8. 2006
  9. ncbi Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains
    J J Chou
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Nat Struct Biol 8:990-7. 2001
  10. ncbi Structure of PTB bound to RNA: specific binding and implications for splicing regulation
    Florian C Oberstrass
    Institute for Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, Zurich, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 309:2054-7. 2005

Research Grants

  1. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2006
  2. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
  3. NATIONAL BIOMEDICAL NMR RESOURCE AT MADISON
    JOHN LUTE MARKLEY; Fiscal Year: 2013
  4. NATIONAL BIOMEDICAL NMR RESOURCE AT MADISON
    John L Markley; Fiscal Year: 2011
  5. Molecular Target Focused Discovery of Anticancer Drugs
    George R Pettit; Fiscal Year: 2010
  6. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2007
  7. 900 MHZ NMR SPECTROMETER
    John Markley; Fiscal Year: 2001
  8. Integrated state-of-the-art LC-MS/NMR system for the University of Wisconsin
    John Markley; Fiscal Year: 2008
  9. Center for Eukaryotic Structural Genomics
    John Markley; Fiscal Year: 2004
  10. 900 MHz NMR Spectrometer Biomolecular Investigations
    John Markley; Fiscal Year: 2006

Detail Information

Publications331 found, 100 shown here

  1. pmc BioMagResBank
    Eldon L Ulrich
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Nucleic Acids Res 36:D402-8. 2008
    ..protein.osaka-u.ac.jp) and the other at CERM, University of Florence, Florence, Italy (bmrb.postgenomicnmr.net/). The site at Osaka also accepts and processes data depositions...
  2. ncbi Automated NMR structure calculation with CYANA
    Peter Güntert
    RIKEN Genomic Sciences Center, Yokohama, Japan
    Methods Mol Biol 278:353-78. 2004
    ....
  3. ncbi The Xplor-NIH NMR molecular structure determination package
    Charles D Schwieters
    Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Building 12A, 20892 5624, Bethesda, MD, USA
    J Magn Reson 160:65-73. 2003
    ..Support for these scripting languages also facilitates interaction with existing external programs for structure analysis, structure manipulation, visualization, and spectral analysis...
  4. ncbi Targeted profiling: quantitative analysis of 1H NMR metabolomics data
    Aalim M Weljie
    Chenomx Inc, Edmonton, Alberta, Canada, and Metabolomics Research Centre, University of Calgary, Calgary, Canada
    Anal Chem 78:4430-42. 2006
    ..We discuss how targeted profiling can be applied for mixture analysis and examine the effect of various acquisition parameters on the accuracy of quantification...
  5. pmc Not so crystal clear: the structure of the human telomere G-quadruplex in solution differs from that present in a crystal
    Jing Li
    Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216 4505, USA
    Nucleic Acids Res 33:4649-59. 2005
    ..These models suggest that the biologically relevant structure of the human telomere quadruplex in K+ solution is not the one determined in the published crystalline state...
  6. ncbi Evaluating protein structures determined by structural genomics consortia
    Aneerban Bhattacharya
    Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers University and Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Proteins 66:778-95. 2007
    ..Potential reasons for this "structure quality score gap" between NMR and X-ray crystal structures are discussed...
  7. pmc HMDB: the Human Metabolome Database
    David S Wishart
    Department of Computing Science, University of Alberta, Edmonton, AB, Canada T6G 2E8
    Nucleic Acids Res 35:D521-6. 2007
    ..The HMDB is designed to address the broad needs of biochemists, clinical chemists, physicians, medical geneticists, nutritionists and members of the metabolomics community. The HMDB is available at: www.hmdb.ca...
  8. pmc An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human BCL-2 promoter region in solution
    Jixun Dai
    College of Pharmacy, University of Arizona, Tucson, 85721, USA
    J Am Chem Soc 128:1096-8. 2006
    ..This predominant bcl-2 G-quadruplex represents an attractive novel target for the design of new anticancer drugs that specifically modulate bcl-2 gene expression...
  9. ncbi Solution structure of Ca(2+)-calmodulin reveals flexible hand-like properties of its domains
    J J Chou
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Nat Struct Biol 8:990-7. 2001
    ..In contrast, the vast majority of side chains making up the target binding surface are locked into the same chi(1) rotameric states as in complexes with target peptide...
  10. ncbi Structure of PTB bound to RNA: specific binding and implications for splicing regulation
    Florian C Oberstrass
    Institute for Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, Zurich, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 309:2054-7. 2005
    ..Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor...
  11. ncbi Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d
    Brian P Hudson
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 11:257-64. 2004
    ..The TIS11d structure provides insights into the RNA-binding functions of this large family of CCCH zinc finger proteins...
  12. ncbi Evaluation of the orthogonal projection on latent structure model limitations caused by chemical shift variability and improved visualization of biomarker changes in 1H NMR spectroscopic metabonomic studies
    Olivier Cloarec
    Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College London, South Kensington, London SW7 2AZ, UK
    Anal Chem 77:517-26. 2005
    ....
  13. pmc Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution
    Attila Ambrus
    College of Pharmacy, The University of Arizona, 1703 E Mabel St, Tucson, AZ 85721, USA
    Nucleic Acids Res 34:2723-35. 2006
    ....
  14. ncbi Combined chemical shift changes and amino acid specific chemical shift mapping of protein-protein interactions
    Frank H Schumann
    Institute of Biophysics and Physical Biochemistry, University of Regensburg, 93040 Regensburg, Germany
    J Biomol NMR 39:275-89. 2007
    ..As we show combined chemical shifts can also be applied for a more reliable quantitative evaluation of titration data...
  15. ncbi Probabilistic quotient normalization as robust method to account for dilution of complex biological mixtures. Application in 1H NMR metabonomics
    Frank Dieterle
    F Hoffman La Roche Ltd, Pharmaceuticals Division, PRBD E, Building 065 512, 4070 Basel, Switzerland
    Anal Chem 78:4281-90. 2006
    ....
  16. ncbi SOFAST-HMQC experiments for recording two-dimensional heteronuclear correlation spectra of proteins within a few seconds
    Paul Schanda
    Institut de Biologie Structurale Jean Pierre Ebel, UMR5075 CNRS CEA UJF, 41, rue Jules Horowitz, 38027, Grenoble Cedex, France
    J Biomol NMR 33:199-211. 2005
    ....
  17. ncbi Automated structure determination from NMR spectra
    Peter Güntert
    Institute of Biophysical Chemistry, Goethe University Frankfurt am Main, Max von Laue Str 9, 60438 Frankfurt am Main, Germany
    Eur Biophys J 38:129-43. 2009
    ....
  18. ncbi Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA
    Torsten Herrmann
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Honggerberg, CH 8093 Zurich, Switzerland
    J Mol Biol 319:209-27. 2002
    ....
  19. ncbi Small change in a G-rich sequence, a dramatic change in topology: new dimeric G-quadruplex folding motif with unique loop orientations
    Martin Crnugelj
    NMR Center, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    J Am Chem Soc 125:7866-71. 2003
    ..Further examination of the influence of different monovalent cations on the folding of d(G(3)T(4)G(4)) showed that it forms a bimolecular G-quadruplex in the presence of K+, Na+, and NH4+ ions with the same general fold...
  20. pmc High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA
    Senada Nozinovic
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 38:683-94. 2010
    ..Here, we discuss the impact of various NMR restraints on structure quality and discuss in detail the dynamics of this system as previously determined...
  21. ncbi Linkage between dynamics and catalysis in a thermophilic-mesophilic enzyme pair
    Magnus Wolf-Watz
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Struct Mol Biol 11:945-9. 2004
    ..This comparative and quantitative study of activity, structure and dynamics revealed a close link between protein dynamics and catalytic turnover...
  22. ncbi Comparison of multiple Amber force fields and development of improved protein backbone parameters
    Viktor Hornak
    Center for Structural Biology, Stony Brook University, Stony Brook, New York 11794, USA
    Proteins 65:712-25. 2006
    ..It also accomplishes improved agreement with published experimental data for conformational preferences of short alanine peptides and better accord with experimental NMR relaxation data of test protein systems...
  23. pmc Structure and mechanism of the M2 proton channel of influenza A virus
    Jason R Schnell
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 451:591-5. 2008
    ..Drug-resistance mutations are predicted to counter the effect of drug binding by either increasing the hydrophilicity of the pore or weakening helix-helix packing, thus facilitating channel opening...
  24. ncbi New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes
    Robert D Peterson
    Department of Chemistry and Biochemistry, and Molecular Biology Institute, University of California, Los Angeles, CA 90095 1569, U S A
    J Biomol NMR 28:59-67. 2004
    ..These experiments are also useful for identifying intermolecular NOEs in RNA-protein complexes. Applications to NOE assignment of larger RNAs and an RNA-protein complex are presented...
  25. pmc The structure of phospholamban pentamer reveals a channel-like architecture in membranes
    Kirill Oxenoid
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:10870-5. 2005
    ..The dynamic N-terminal amphipathic helices point away from the membrane, perhaps facilitating recognition and inhibition of the calcium pump...
  26. ncbi NMR characterization of the dynamics of biomacromolecules
    Arthur G Palmer
    Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Chem Rev 104:3623-40. 2004
  27. ncbi Metabonomic and microbiological analysis of the dynamic effect of vancomycin-induced gut microbiota modification in the mouse
    Ivan K S Yap
    Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Proteome Res 7:3718-28. 2008
    ....
  28. pmc An extended structure of the APOBEC3G catalytic domain suggests a unique holoenzyme model
    Elena Harjes
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, 55455, USA
    J Mol Biol 389:819-32. 2009
    ....
  29. pmc Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model
    Francois Pierre J Martin
    Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, London, UK
    Mol Syst Biol 4:157. 2008
    ....
  30. pmc Crystal structure of the APOBEC3G catalytic domain reveals potential oligomerization interfaces
    Shivender M D Shandilya
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Structure 18:28-38. 2010
    ..NMR solution data provides evidence that another interface, which coordinates a novel zinc site, also exists. Thus, the observed crystallographic interfaces of APOBEC3G may be important for both oligomerization and function...
  31. ncbi Cross-correlated relaxation enhanced 1H[bond]13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes
    Vitali Tugarinov
    Protein Engineering Network Centers of Excellence and Department of Medical Genetics, The University of Toronto, Toronto, Ontario, Canada M5S 1A8
    J Am Chem Soc 125:10420-8. 2003
    ..The high quality of HMQC spectra obtained in short measuring times strongly suggests that methyl groups will be useful probes of structure and dynamics in supramolecular complexes...
  32. ncbi General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy
    Jihui Wu
    Department of Biological Sciences and Department of Chemistry, National University of Singapore, 14 Science Drive 4, Singapore 117543
    J Am Chem Soc 126:15018-9. 2004
    ....
  33. ncbi Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure
    Anna Huppler
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Nat Struct Biol 9:431-5. 2002
    ..A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing...
  34. ncbi Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein
    Qiulong Huang
    Global Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, Illinois 60064 6098, USA
    Biochemistry 43:6059-63. 2004
    ..Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site...
  35. ncbi DNA-induced alpha-helical structure in the NH2-terminal domain of histone H1
    R Vila
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, , 08193 Bellaterra, Barcelona, Spain
    J Biol Chem 276:46429-35. 2001
    ..In chromatin, the high positive charge density of the inducible NH(2)-terminal helical element may contribute to the binding stability of the globular domain...
  36. ncbi NMR R1 rho rotating-frame relaxation with weak radio frequency fields
    Francesca Massi
    Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, New York 10032, USA
    J Am Chem Soc 126:2247-56. 2004
    ..The new experiments also extend the capability of the R(1 rho) technique to study exchange processes outside the fast exchange limit...
  37. pmc Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence
    Jixun Dai
    College of Pharmacy, The University of Arizona, 1703 E Mabel St, Tucson, AZ 85721, USA
    Nucleic Acids Res 35:4927-40. 2007
    ....
  38. ncbi MUNIN: a new approach to multi-dimensional NMR spectra interpretation
    V Y Orekhov
    Swedish NMR Centre at Göteborg University
    J Biomol NMR 20:49-60. 2001
    ..As a result, the extraction of structural information simply consists of one-dimensional peak picking of the shape along the NOE-axis obtained for each amide group...
  39. ncbi Fast time scale dynamics of protein backbones: NMR relaxation methods, applications, and functional consequences
    Virginia A Jarymowycz
    Department of Chemistry and Interdisciplinary Biochemistry Program, Indiana University, Bloomington, Indiana 47405 0001, USA
    Chem Rev 106:1624-71. 2006
  40. ncbi NMR analysis of a 900K GroEL GroES complex
    Jocelyne Fiaux
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
    Nature 418:207-11. 2002
    ..This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes...
  41. ncbi Solution structure of the Reps1 EH domain and characterization of its binding to NPF target sequences
    S Kim
    Department of Biochemistry, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Biochemistry 40:6776-85. 2001
    ..In addition, we characterized binding to a peptide containing a DPF sequence (Kd = 0.5 +/- 0.2 mM). The characterization of binding between the Reps1 EH domain and its target proteins provides information about their role in endocytosis...
  42. ncbi Probing the conformational heterogeneity of the acetylaminofluorene-modified 2'-deoxyguanosine and DNA by 19F NMR spectroscopy
    B P Cho
    Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kingston 02881, USA
    Biochemistry 38:7572-83. 1999
    ..et al. (1997) J. Am. Chem. Soc. 119, 5384-5389]. The exclusive stacked nature of the AAF adducts may provide insight into why AAF adducts are more mutagenic and prone to repair than the nonacetylated AF adducts...
  43. ncbi An intramolecular quadruplex of (GGA)(4) triplet repeat DNA with a G:G:G:G tetrad and a G(:A):G(:A):G(:A):G heptad, and its dimeric interaction
    A Matsugami
    Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama, 240-8501, Japan
    J Mol Biol 313:255-69. 2001
    ..This is in contrast to the case of d(GGAGGAN) (N=G or T), which forms a duplex under low salt concentration conditions. On the basis of these results, the structure of naturally occurring GGA triplet repeat DNA is discussed...
  44. ncbi Structure of Bax: coregulation of dimer formation and intracellular localization
    M Suzuki
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 103:645-54. 2000
    ..The Bax structure shows that the orientation of helix alpha 9 provides simultaneous control over its mitochondrial targeting and dimer formation...
  45. pmc Structural model and functional significance of pH-dependent talin-actin binding for focal adhesion remodeling
    J Srivastava
    Department of Cell and Tissue Biology, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 105:14436-41. 2008
    ..These data identify a molecular mechanism for pH-sensitive actin binding by talin and suggest that FA turnover is pH-dependent and in part mediated by pH-dependent affinity of talin for binding actin...
  46. ncbi Using NMRView to visualize and analyze the NMR spectra of macromolecules
    Bruce A Johnson
    Molecular Systems, Merck Research Labs, Rahway, NJ, USA
    Methods Mol Biol 278:313-52. 2004
    ..NMR spectral peaks can be analyzed and assigned. A suite of tools exists within NMRView for assigning the data from triple-resonance experiments...
  47. ncbi Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif
    D J Craik
    Centre for Drug Design and Development, The University of Queensland, Brisbane, QLD 4072, Australia
    J Mol Biol 294:1327-36. 1999
    ..Here we define the structural features of the two apparent subfamilies of the CCK peptides which may be significant for the likely defense related role of these peptides within plants...
  48. pmc Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase
    Wade D Van Horn
    Department of Biochemistry and Center for Structural Biology, Vanderbilt University, Nashville, TN 37232, USA
    Science 324:1726-9. 2009
    ..Mutations to cysteine that caused severe misfolding were located in or near the active site, indicating a high degree of overlap between sites responsible for folding and for catalysis...
  49. ncbi Inferential structure determination
    Wolfgang Rieping
    Unite de Bioinformatique Structurale, Institut Pasteur, CNRS URA 2185, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Science 309:303-6. 2005
    ..We implemented this approach by using Markov chain Monte Carlo techniques. Our method provides an objective figure of merit and improves structural quality...
  50. pmc The NMR restraints grid at BMRB for 5,266 protein and nucleic acid PDB entries
    Jurgen F Doreleijers
    Radboud University Medical Centre Nijmegen, Geert Grooteplein 26 28, HB, Nijmegen, The Netherlands
    J Biomol NMR 45:389-96. 2009
    ..Continuous efforts in automating the parsing of both old, and newly deposited files is steadily decreasing this fraction. The cleaned data files are available from the NMR restraints grid at http://restraintsgrid.bmrb.wisc.edu ...
  51. ncbi Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts
    Olaf Beckonert
    Department of Biomolecular Medicine, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, UK
    Nat Protoc 2:2692-703. 2007
    ....
  52. ncbi Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3
    Hugo van Ingen
    Bijvoet Centre for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands
    Structure 16:1245-56. 2008
    ..Interference by asymmetric dimethylation of arginine 2 suggests that a H3R2/K4 "methyl-methyl" switch in the histone code dynamically regulates TFIID-promoter association...
  53. ncbi Structural basis of integrin activation by talin
    Kate L Wegener
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England, UK
    Cell 128:171-82. 2007
    ....
  54. ncbi Amyloid fibrils of the HET-s(218-289) prion form a beta solenoid with a triangular hydrophobic core
    Christian Wasmer
    Physical Chemistry, ETH Zurich, 8093 Zurich, Switzerland
    Science 319:1523-6. 2008
    ..The structure is likely to have broad implications for understanding the infectious amyloid state...
  55. pmc Inhibitors of amyloid toxicity based on beta-sheet packing of Abeta40 and Abeta42
    Takeshi Sato
    Department of Biochemistry and Cell Biology, Center for Structural Biology, Stony Brook University, Stony Brook, New York 11794 5215, USA
    Biochemistry 45:5503-16. 2006
    ..Importantly, the designed peptide inhibitors significantly reduce the toxicity induced by Abeta42 on cultured rat cortical neurons...
  56. ncbi Correlation between the occurrence of 1H-MRS lipid signal, necrosis and lipid droplets during C6 rat glioma development
    Sonja Zoula
    Laboratoire mixte INSERM U438 Université Joseph Fourier, Laboratoire de Recherche Correspondant du CEA, Centre Hospitalier Universitaire, Grenoble, France
    NMR Biomed 16:199-212. 2003
    ..These necrotic areas contained large amounts of lipid droplets. All these results suggest that mobile lipids detected in vivo by 1H MRS (136 ms echo time) in C6 rat brain glioma arise mainly from lipid droplets located in necrosis...
  57. ncbi PRIMe: a Web site that assembles tools for metabolomics and transcriptomics
    Kenji Akiyama
    RIKEN Plant Science Center, Yokohama, Kanagawa, Japan
    In Silico Biol 8:339-45. 2008
    ....
  58. ncbi A site-specific low-enrichment (15)N,(13)C isotope-labeling approach to unambiguous NMR spectral assignments in nucleic acids
    Anh Tuân Phan
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center New York, New York 10021, USA
    J Am Chem Soc 124:1160-1. 2002
    ..This simple and cost-effective methodology is demonstrated on the d(GGGTTCAGG) DNA sequence, which forms a dimeric G-quadruplex containing G.G.G.G tetrads sandwiched between G.(C-A) triads...
  59. pmc Measurement of bond vector orientations in invisible excited states of proteins
    Pramodh Vallurupalli
    Department of Molecular and Medical Genetics, University of Toronto, Toronto, ON, Canada
    Proc Natl Acad Sci U S A 104:18473-7. 2007
    ....
  60. pmc Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III
    Haihong Wu
    Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, CA 90095 1569, USA
    Proc Natl Acad Sci U S A 101:8307-12. 2004
    ..These results show how a single dsRBD can convey specificity for particular RNA targets, by structure specific recognition of a conserved tetraloop fold...
  61. ncbi 1H NMR metabonomics can differentiate the early atherogenic effect of dairy products in hyperlipidemic hamsters
    Jean Charles Martin
    Institut National de la Recherche Agronomique, UMR1260 Nutriments Lipidiques et Prevention des Maladies Metaboliques, Marseille, France
    Atherosclerosis 206:127-33. 2009
    ..This proof-of-principle study demonstrates the first use of plasma metabonomics for improving the prognosis of diet-induced atherogenesis, revealing novel potential disease biomarkers...
  62. ncbi Structures and mechanisms of Nudix hydrolases
    A S Mildvan
    Department of Biological Chemistry, The Johns Hopkins School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205 2185, USA
    Arch Biochem Biophys 433:129-43. 2005
    ..The overall rate accelerations can be explained by both independent and cooperative effects of these catalytic components...
  63. pmc Constructing RNA dynamical ensembles by combining MD and motionally decoupled NMR RDCs: new insights into RNA dynamics and adaptive ligand recognition
    Aaron T Frank
    Department of Chemistry, University of California Irvine, 1102 Natural Sciences 2, Irvine, CA 92697, USA
    Nucleic Acids Res 37:3670-9. 2009
    ....
  64. ncbi Conformational averaging in structural biology: issues, challenges and computational solutions
    Daniela Kruschel
    Laboratory of Computational Biophysics, Mediterranean Institute for Life Sciences, Mestrovicevo setaliste bb, Split, HR 21000, Croatia
    Mol Biosyst 5:1606-16. 2009
    ....
  65. ncbi Differential responses of the backbone and side-chain conformational dynamics in FKBP12 upon binding the transition-state analog FK506: implications for transition-state stabilization and target protein recognition
    Ulrika Brath
    Division of Biophysical Chemistry, Center for Molecular Protein Science, Lund University, Lund, Sweden
    J Mol Biol 387:233-44. 2009
    ....
  66. ncbi Structure of the C-terminal MA-3 domain of the tumour suppressor protein Pdcd4 and characterization of its interaction with eIF4A
    L C Waters
    Department of Biochemistry, University of Leicester, Leicester, UK
    Oncogene 26:4941-50. 2007
    ..This site is strongly conserved in other MA-3 domains known to interact with eIF4A, including the preceding domain of Pdcd4, suggesting a common mode of binding...
  67. pmc Metal-ion binding and metal-ion induced folding of the adenine-sensing riboswitch aptamer domain
    Jonas Noeske
    Department of Biochemistry, The University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA
    Nucleic Acids Res 35:5262-73. 2007
    ..Compared to the highly similar guanine-sensing riboswitch, the folding pathway for the adenine-sensing riboswitch aptamer domain is more complex and the influence of Mg2+ is more pronounced...
  68. pmc NMR and biochemical characterization of recombinant human tRNA(Lys)3 expressed in Escherichia coli: identification of posttranscriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription
    C Tisne
    Laboratoire de Cristallographie et Résonance Magnétique Nucléaire Biologiques, Faculte de Pharmacie, Centre National de la Recherche Scientifique Equipe Postulante 2075, Paris, France
    RNA 6:1403-12. 2000
    ..coli (m2G10, psi27, m5C48, m5C49, and m1A58) as well as the mnm5 group at position 34 are dispensable for initiation of reverse transcription. In contrast, the 2-thio group at position 34 seems to play an important part in this process...
  69. pmc Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation
    Shivani Ahuja
    Department of Physics and Astronomy, Stony Brook University, Stony Brook, New York 11794 5215, USA
    Nat Struct Mol Biol 16:168-75. 2009
    ..These results provide a comprehensive view of how retinal isomerization triggers helix motion and activation in this prototypical G protein-coupled receptor...
  70. pmc Toward a unified representation of protein structural dynamics in solution
    Phineus R L Markwick
    Protein Dynamics and Flexibility, Institute de Biologie Structurale Jean Pierre Ebel, CNRS CEA UJF UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France
    J Am Chem Soc 131:16968-75. 2009
    ..This constraint-free approach thereby provides an atomic resolution free-energy weighted Boltzmann description of protein dynamics occurring on time scales over many orders of magnitude in the protein ubiquitin...
  71. pmc SCOR: a Structural Classification of RNA database
    Peter S Klosterman
    Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Nucleic Acids Res 30:392-4. 2002
    ..A classification of the well-characterized tertiary interactions found in the larger RNA structures is also included along with examples. The SCOR database is accessible at http://scor.lbl.gov...
  72. ncbi Structure of the DNA deaminase domain of the HIV-1 restriction factor APOBEC3G
    Kuan Ming Chen
    Department of Biochemistry, Molecular Biology and Biophysics, of Minnesota, Minneapolis, Minnesota 55455, USA
    Nature 452:116-9. 2008
    ..The structure of the APOBEC3G catalytic domain will help us to understand functions of other family members and interactions that occur with pathogenic proteins such as HIV-1 Vif...
  73. ncbi Determination of an ensemble of structures representing the denatured state of the bovine acyl-coenzyme a binding protein
    Kresten Lindorff-Larsen
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom
    J Am Chem Soc 126:3291-9. 2004
    ..The preferential formation of these contacts suggests that the sequence-dependent patterns of helical propensity and hydrophobicity are important determinants of the structure in the denatured state of ACBP...
  74. ncbi Heteronuclear NMR studies of the interaction of tRNA(Lys)3 with HIV-1 nucleocapsid protein
    C Tisne
    Laboratoire de Cristallographie et RMN Biologiques, EP 2075 CNRS Faculté de Pharmacie, 4 avenue de l Observatoire, Paris, 75006, France
    J Mol Biol 306:443-54. 2001
    ..It is interesting that for the biological role of the NC protein, these pairs could be the starting points of the tRNA melting required for the hybridisation to the viral RNA...
  75. pmc Structural biology of transmembrane domains: efficient production and characterization of transmembrane peptides by NMR
    Jian Hu
    Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306, USA
    Protein Sci 16:2153-65. 2007
    ..The high yield, the simplicity of purification, and the convenient protocol represents a suitable approach for NMR studies and a starting point for characterizing the transmembrane domains of membrane proteins...
  76. ncbi Automated NMR determination of protein backbone dihedral angles from cross-correlated spin relaxation
    Karin Kloiber
    Institute of Organic Chemistry, University of Innsbruck, Austria
    J Biomol NMR 22:349-63. 2002
    ..The approach is illustrated with an application to 13C, 15N-labeled ubiquitin...
  77. pmc Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR
    Alexander N Volkov
    Leiden Institute of Chemistry, Leiden University, Gorlaeus Laboratories, P O Box 9502, 2300 RA Leiden, The Netherlands
    Proc Natl Acad Sci U S A 103:18945-50. 2006
    ..Our findings corroborate the dynamic behavior of this complex and offer an insight into the mechanism of the protein complex formation in solution...
  78. ncbi The structural and dynamic basis of Ets-1 DNA binding autoinhibition
    Gregory M Lee
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 280:7088-99. 2005
    ..Ets-1 autoinhibition illustrates how conformational equilibria within structural domains can regulate macromolecular interactions...
  79. ncbi Application of NMR in structural proteomics: screening for proteins amenable to structural analysis
    Till Rehm
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 München, Germany
    Structure 10:1613-8. 2002
    ..NMR can thus provide important information for optimizing conditions for protein constructs that are amenable to structural studies...
  80. ncbi Quantitative dynamics and binding studies of the 20S proteasome by NMR
    Remco Sprangers
    Department of Biochemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Nature 445:618-22. 2007
    ..Our results establish that NMR spectroscopy can provide detailed insight into supra-molecular structures over an order of magnitude larger than those routinely studied using methodology that is generally applicable...
  81. ncbi Metabonomic investigations of aging and caloric restriction in a life-long dog study
    Yulan Wang
    Department of Biomolecular Medicine, SORA Division, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, United Kingdom
    J Proteome Res 6:1846-54. 2007
    ....
  82. pmc Prediction of charge-induced molecular alignment of biomolecules dissolved in dilute liquid-crystalline phases
    Markus Zweckstetter
    Max Planck Institute for Biophysical Chemistry, Am Fassberg, Gottingen, Germany
    Biophys J 86:3444-60. 2004
    ..Inclusion of electrostatic interactions in addition to steric effects makes the extended model applicable to all liquid crystals used in biological NMR to date...
  83. ncbi FAST-Modelfree: a program for rapid automated analysis of solution NMR spin-relaxation data
    Roger Cole
    Department of Chemistry, Yale University, P O Box 208107, New Haven, CT 06520, USA
    J Biomol NMR 26:203-13. 2003
    ..In all cases results obtained with FAST-Modelfree compared favorably with the original literature results...
  84. pmc Structural and dynamic characterization of an unfolded state of poplar apo-plastocyanin formed under nondenaturing conditions
    Y Bai
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Protein Sci 10:1056-66. 2001
    ....
  85. pmc Characterization of conformational and dynamic properties of natively unfolded human and mouse alpha-synuclein ensembles by NMR: implication for aggregation
    Kuen Phon Wu
    Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854, USA
    J Mol Biol 378:1104-15. 2008
    ..These transient interchain interactions coupled with a non-A beta amyloid component (NAC) region that is both more exposed and has a higher propensity to beta structure may accelerate the rate of fibril formation of aS...
  86. ncbi Solid-state NMR spectroscopy reveals that E. coli inclusion bodies of HET-s(218-289) are amyloids
    Christian Wasmer
    Laboratorium fur Physikalische Chemie, ETH Zurich, 8093 Zurich, Switzerland
    Angew Chem Int Ed Engl 48:4858-60. 2009
    ..coli IBs of the prion-forming domain from the fungal prion HET-s is the same as that previously determined for fibrils assembled in vitro, and show prion infectivity. These results demonstrate that the IBs of HET-s(218-289) are amyloids...
  87. ncbi A phase cycle scheme that significantly suppresses offset-dependent artifacts in the R2-CPMG 15N relaxation experiment
    Grover N B Yip
    Department of Chemistry, University of Michigan Biophysics Research Division 930 N University Ave, Ann Arbor, MI 48109, USA
    J Magn Reson 171:25-36. 2004
    ....
  88. ncbi Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA:RNA hybrids
    Michael Petersen
    Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Odense University, DK 5230 Odense M, Denmark
    J Am Chem Soc 124:5974-82. 2002
    ....
  89. ncbi Complete protein structure determination using backbone residual dipolar couplings and sidechain rotamer prediction
    Michael Andrec
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, NJ 08854 8087, USA
    J Struct Funct Genomics 2:103-11. 2002
    ....
  90. ncbi Experimental and analytical variation in human urine in 1H NMR spectroscopy-based metabolic phenotyping studies
    Anthony D Maher
    Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics SORA, Faculty of Medicine, Imperial College London, South Kensington SW7 2AZ, United Kingdom
    Anal Chem 79:5204-11. 2007
    ....
  91. pmc Defining the role of active-site loop fluctuations in dihydrofolate reductase catalysis
    Dan McElheny
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:5032-7. 2005
    ....
  92. pmc Probing the sequence effects on NarI-induced -2 frameshift mutagenesis by dynamic 19F NMR, UV, and CD spectroscopy
    Nidhi Jain
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA
    Biochemistry 46:13310-21. 2007
    ..We also provide evidence for AF/FAF conformational compatibility in the NarI sequences...
  93. pmc Conformation of alamethicin in oriented phospholipid bilayers determined by (15)N solid-state nuclear magnetic resonance
    M Bak
    Laboratory for Biomolecular NMR Spectroscopy, Department of Molecular and Structural Biology, Science Park, University of Aarhus, DK-8000 Aarhus C, Denmark
    Biophys J 81:1684-98. 2001
    ..300:325-330, 1982). The solid-state NMR-compatible structures may support the formation of a left-handed and parallel multimeric ion channel...
  94. pmc Structural basis of site-specific histone recognition by the bromodomains of human coactivators PCAF and CBP/p300
    Lei Zeng
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York University, New York, NY 10029, USA
    Structure 16:643-52. 2008
    ....
  95. ncbi NMR structure of the full-length linear dimer of stem-loop-1 RNA in the HIV-1 dimer initiation site
    Nikolai B Ulyanov
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA
    J Biol Chem 281:16168-77. 2006
    ..These guanines may be recognized by the nucleocapsid protein, which binds tightly to the G-bulge in vitro...
  96. ncbi The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution
    F Mueller
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, Berlin, 14195, Germany
    J Mol Biol 298:35-59. 2000
    ....
  97. pmc Characterization of the fast dynamics of protein amino acid side chains using NMR relaxation in solution
    Tatyana I Igumenova
    Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6059, USA
    Chem Rev 106:1672-99. 2006
  98. pmc A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III
    H Wu
    Department of Chemistry and Biochemistry, 405 Hilgard Avenue, PO Box 951569, University of California, Los Angeles, CA 90095-1569, USA
    EMBO J 20:7240-9. 2001
    ....
  99. pmc Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunit
    Geoffrey A Mueller
    Laboratory of Structural Biology, MR 01, National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 187:7081-9. 2005
    ..The new theta structure is expected to provide additional insight into its physiological role and its effect on the epsilon proofreading subunit...
  100. ncbi Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex
    Barbara Beuth
    Division of Molecular Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U K
    J Am Chem Soc 129:10205-10. 2007
    ..Our NMR binding assays using the third KH domain of the Nova-1 protein provide a proof-of-principle for the method and novel information on the specificity of this domain for its RNA targets...
  101. ncbi Competitive Na(+) and Rb(+) binding in the minor groove of DNA
    Flaminia Cesare Marincola
    Department of Biophysical Chemistry, Lund University, SE 22100 Lund, Sweden
    J Am Chem Soc 126:6739-50. 2004
    ..At 4 degrees C, the mean residence time of groove-bound ions is 0.2 +/- 0.1 micros for Rb(+) and 10 ns to 100 micros for Na(+). A shorter correlation time of 2 ns is attributed to counterions bridging cross-strand phosphate groups...

Research Grants65

  1. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2006
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  2. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..unreadable] [unreadable] All the major users of the instrument are supported by NIH R01 grants. [unreadable] [unreadable] [unreadable]..
  3. NATIONAL BIOMEDICAL NMR RESOURCE AT MADISON
    JOHN LUTE MARKLEY; Fiscal Year: 2013
    The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource for biomolecular nuclear magnetic resonance (NMR) spectroscopy...
  4. NATIONAL BIOMEDICAL NMR RESOURCE AT MADISON
    John L Markley; Fiscal Year: 2011
    The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource for biomolecular nuclear magnetic resonance (NMR) spectroscopy...
  5. Molecular Target Focused Discovery of Anticancer Drugs
    George R Pettit; Fiscal Year: 2010
    ....
  6. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2007
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs direct at improving human cancer treatments. ..
  7. 900 MHZ NMR SPECTROMETER
    John Markley; Fiscal Year: 2001
    ..abstract_text> ..
  8. Integrated state-of-the-art LC-MS/NMR system for the University of Wisconsin
    John Markley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  9. Center for Eukaryotic Structural Genomics
    John Markley; Fiscal Year: 2004
    ..thaliana to be funded separately. The methods developed by the Center will be applicable to other eukaryotic genomes, including the human genome. ..
  10. 900 MHz NMR Spectrometer Biomolecular Investigations
    John Markley; Fiscal Year: 2006
    ..Web-based software will be used to pair users with local collaborator(s), assess specific needs, design and schedule data collection, and share results. ..
  11. NMR Spectrometer System Optimized for Direct 13C or 15N Detection
    John Markley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  12. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2009
    ....
  13. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2008
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  14. CONFERENCE ON MAGNETIC RESONANCE IN BIOLOGICAL SYSTEMS
    John Markley; Fiscal Year: 2002
    ..Those receiving travel support will be selected by a committee composed of the U.S. members of the International Organizing Committee for the meeting. ..
  15. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John L Markley; Fiscal Year: 2010
    ..abstract_text> ..
  16. EFFECT OF PB2+ TOXICITY AT THE MOLECULAR LEVEL
    TERRY DOWD; Fiscal Year: 2009
    ..abstract_text> ..
  17. BcI-2 family proteins as targets for chemoprevention an*
    Maurizio Pellecchia; Fiscal Year: 2005
    ....
  18. DNA Damage: Roles in Toxicity and Mutagenicity
    Barry Gold; Fiscal Year: 2008
    ..The results of the above aims should provide useful information on the toxicity and tumorigenicity of 3-MeA and related DNA lesions, as well as how tumorigenicity can be avoided to afford improved and safer cancer chemotherapies. ..
  19. Dynamics, Function, and Stability in Large Enzymes
    JOSEPH LORIA; Fiscal Year: 2009
    ..This combination of experiments will allow characterization of the dynamics of catalytically important residues and the active site loop. ..
  20. MOLECULAR BASIS OF LOCAL ANESTHESIA
    Walter Chazin; Fiscal Year: 2008
    ....
  21. Biomimetic Studies of NO-binding Respiratory Chain Hemes
    JAMES COLLMAN; Fiscal Year: 2008
    ..An emphasis will be placed on kinetic and mechanistic studies to elucidate the putitive reaction pathways of CcO and NOR. ..
  22. ATP-Ubiquitin Dependent Proteolysis
    Arthur L Haas; Fiscal Year: 2011
    ....
  23. Oxygen-linked Phenol-DNA Adducts
    SHANA STURLA; Fiscal Year: 2008
    ..The research environment presents faculty expertise and major instrumentation ideally suited for the proposed work. ..
  24. Structure and Function of Tau
    David Eliezer; Fiscal Year: 2008
    ..This work may also have broader implications for understanding and treating other protein aggregation diseases. ..
  25. 31st NATIONAL MEDICINAL CHEMISTRY SYMPOSIUM
    Barry Gold; Fiscal Year: 2008
    ..NIH funds will be used to reimburse travel award recipients for the registration fee, campus lodging, awards banquet, and airfare. [unreadable] [unreadable] [unreadable] [unreadable]..
  26. Structure and Function of Alpha-Synuclein
    David Eliezer; Fiscal Year: 2010
    ....
  27. ANTIFUNGALS FROM MARINE INVERTEBRATES--AIDS ADJUVANTS
    Tadeusz F Molinski; Fiscal Year: 2010
    ..Optimized leads identified from those libraries will be advanced to in vitro and in vivo trials. ..
  28. Anti-apoptotic compounds for treatment of brain ischemia
    Maurizio Pellecchia; Fiscal Year: 2010
    ..abstract_text> ..
  29. SYNTHESIS OF ANTICANCER AGENTS
    K C Nicolaou; Fiscal Year: 2010
    ..abstract_text> ..
  30. THE PHAGOCYTE MEMBRANE
    M Amin Arnaout; Fiscal Year: 2009
    ..3) Identify novel intermediates that regulate inside-out activation of integrins using genetic (RNAi), immunochemical and biochemical approaches. ..
  31. DESIGN AND SYNTHESIS OF NONPEPTIDE PROTEASE INHIBITORS
    Arun K Ghosh; Fiscal Year: 2010
    ..This research integrates organic synthesis, protein-ligand x-ray crystallography, molecular modeling and in-depth virus and cell-biological studies to design the next generation of HIV-1 protease inhibitors. ..
  32. S100 PROTEINS: STRUCTURAL BASIS OF FUNCTIONAL PROPERTIES
    Walter Chazin; Fiscal Year: 2009
    ..These results will provide the insights on the structural basis of S100 function in AGE receptor signaling, the first such information on function of S100 proteins in the extracellular milieu. ..
  33. Lipoproteins, HIV, and Antiretroviral Therapy in SMART
    Daniel Duprez; Fiscal Year: 2008
    ..This proposal will produce additional information that will enhance their disease-free survival rate. (End of Abstract) [unreadable] [unreadable] [unreadable]..
  34. Regulation Vascular Smooth Muscle Myosin Phosphatase
    Masumi Eto; Fiscal Year: 2010
    ..Relevance) Drugs, blocking the contraction, are used for treatment of hypertension, asthma and erectile dysfunction. The results of this project will expose potential therapeutic targets for smooth muscle diseases. ..
  35. Cellular Functions of the Human Filamins
    Masumi Eto; Fiscal Year: 2007
    ..abstract_text> ..
  36. Sodium Channel Contratoxins Derived from Cone Snail Venoms
    Grzegorz Bulaj; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  37. DEVELOPMENT OF LIGAND ASSISTED ASYMMETRIC SYNTHESES
    Arun Ghosh; Fiscal Year: 2001
    ..Besides the broad range of scope and generality, this line of research will provide excellent opportunities to teach and train graduate and undergraduate students in the laboratory. ..
  38. DNA TOPOISOMERASE I TARGET INTERACTIONS OF CAMPTOTHECINS
    Danzhou Yang; Fiscal Year: 2004
    ..This research project is intended to define the academic research program of Dr. Yang who is intent on achieving a faculty position in a first- rate research-oriented College of Pharmacy or Medicine in the United States. ..
  39. TOTAL SYNTHESIS OF APOPTOLIDIN
    K Nicolaou; Fiscal Year: 2004
    ..The proposed work is expected to have significant impact in the area of cancer chemotherapy and should provide enabling technologies and tools for biology and medicine. ..
  40. RELATIONSHIP BETWEEN DNA STRUCTURE AND ADDUCT FORMATION
    Barry Gold; Fiscal Year: 2004
    ..abstract_text> ..
  41. FOLDING OF THE INTRACELLULAR LIPID-BINDING PROTEINS
    Ira Ropson; Fiscal Year: 2003
    ..Single-site mutant proteins will be generated and studied for their effects on the transition state in protein folding. ..
  42. INTERFACIAL ACTIVATION OF WATER SOLUBLE PHOSPHOLIPASES
    Mary Roberts; Fiscal Year: 2003
    ..abstract_text> ..
  43. Single Molecule Studies of SNARE-Induced Vesicle Fusion
    Axel T Brunger; Fiscal Year: 2010
    ..Such a system could serve as an efficient model system for novel drug discovery. ..
  44. ABI 3100 Genetic Analyzer for Nucleic Acid Sequencing
    Arthur Haas; Fiscal Year: 2003
    ..In addition, the proposed instrument will allow longer reads than currently available on the ABI 310 instrument. ..
  45. CYTOCHROME P450 SUBSTRATE SITING AND MOTION
    Ann McDermott; Fiscal Year: 2002
    ..Studies involving microbial detoxification enzymes with similar chemical mechanisms are also planned. ..
  46. Intermolecular Multiple-Quantum Coherence Effects in NMR
    Warren Warren; Fiscal Year: 2002
    ....
  47. Proteomics: The Next Grand Biological Challenge
    Walter Chazin; Fiscal Year: 2002
    ..A concentrated effort will be made to attract ethnic minorities, women and persons with disabilities as participants, organizers, and speakers. ..
  48. Two-Photon Absorption Imaging by Laser Pulse Shaping
    Warren Warren; Fiscal Year: 2004
    ..abstract_text> ..
  49. STRUCTURAL BIOLOGY OF EF-HAND CALCIUM BINDING PROTEINS
    Walter Chazin; Fiscal Year: 2005
    ..These results will link the large volume of experimental data available on EF-hand proteins, uncover the mechanisms by which these proteins function, and help to define their biological activities. ..
  50. Allosteric JNK modulators for the treatment of diabetes
    Maurizio Pellecchia; Fiscal Year: 2007
    ..By exploring novel routes for the inhibition of a key modulator of the metabolic disorder, the protein target JNK, we hope to derive new agents to advance novel therapies against obesity and diabetes. [unreadable] [unreadable]..
  51. NMR Structure of Membrane Proteins of T. maritima
    Linda Columbus; Fiscal Year: 2005
    ..In addition, more helical transmembrane structures will aid in the understanding of membrane protein stability and increase the likelihood of predicting membrane protein structures. ..
  52. NMR spectroscopy instrumentation for probing intermolecular interations
    Maurizio Pellecchia; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  53. FUNCTION OF AN INTERFERON INDUCED UBIQUITIN HOMOLOG
    Arthur Haas; Fiscal Year: 2003
    ..These studies are the first concerted functional survey of ISG15 conjugation and the enzymology of this modification. ..
  54. Solid State Nuclear Magnetic Resonance Studies of TIM
    Ann McDermott; Fiscal Year: 2005
    ..abstract_text> ..
  55. Structure-function of the Proangiogenic Integrin alphaVb
    M Amin Arnaout; Fiscal Year: 2006
    ....
  56. KALLIKREINS AS DIAGNOSTIC MARKERS OF OVARIAN CARCINOMA
    Eleftherios Diamandis; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  57. Human Kallikrein 4 (hK4): A New Prostatic Biomarker?
    Eleftherios Diamandis; Fiscal Year: 2003
    ..If successful, our research will contribute to the development of new prostatic biomarkers and possibly, to better diagnosis which may lead to better clinical outcomes and reduced healthcare costs. ..
  58. A New Biomarker for Ovarian Cancer Detection
    Eleftherios Diamandis; Fiscal Year: 2003
    ..In this grant proposal, we describe further studies, which will examine in greater detail, the ability of a test for the amount of this protein in blood to correctly diagnose the presence of ovarian cancer. ..
  59. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2003
    ....
  60. Nanoscale-Natural Products Chemistry
    TADEUSZ MOLINSKI; Fiscal Year: 2009
    ....
  61. Natural Product Antitumor Inducers of Apoptosis
    TADEUSZ MOLINSKI; Fiscal Year: 2004
    ..abstract_text> ..
  62. Nitroreductase: Determinants of Flavin Enzyme Activity
    Anne Frances Miller; Fiscal Year: 2004
    ..These tools will have widespread applicability to flavoproteins. ..
  63. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2008
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  64. Structure-Function Relationships in Lung Surfactants
    Joanna Long; Fiscal Year: 2008
    ..We will be using novel, sensitive solid state NMR experiments which allow us to determine at atomic resolution these parameters at low concentrations in complex lipid environments. ..
  65. NOVEL PROBES FOR IMMUNOPHILIN MEDIATED CELL CONTROL
    TADEUSZ MOLINSKI; Fiscal Year: 2001
    ..5. To understand how modulation of the FKBP12/ryanodine receptor complex by bastadins influences cellular growth and differentiation using the BC3H1 cell line as model. ..