biomolecular nuclear magnetic resonance

Summary

Summary: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.

Top Publications

  1. pmc BioMagResBank
    Eldon L Ulrich
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Nucleic Acids Res 36:D402-8. 2008
  2. ncbi Evaluating protein structures determined by structural genomics consortia
    Aneerban Bhattacharya
    Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers University and Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Proteins 66:778-95. 2007
  3. ncbi The Xplor-NIH NMR molecular structure determination package
    Charles D Schwieters
    Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Building 12A, 20892 5624, Bethesda, MD, USA
    J Magn Reson 160:65-73. 2003
  4. ncbi Structure of PTB bound to RNA: specific binding and implications for splicing regulation
    Florian C Oberstrass
    Institute for Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, Zurich, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 309:2054-7. 2005
  5. ncbi NMR characterization of the dynamics of biomacromolecules
    Arthur G Palmer
    Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Chem Rev 104:3623-40. 2004
  6. ncbi Combined chemical shift changes and amino acid specific chemical shift mapping of protein-protein interactions
    Frank H Schumann
    Institute of Biophysics and Physical Biochemistry, University of Regensburg, 93040 Regensburg, Germany
    J Biomol NMR 39:275-89. 2007
  7. ncbi Probabilistic quotient normalization as robust method to account for dilution of complex biological mixtures. Application in 1H NMR metabonomics
    Frank Dieterle
    F Hoffman La Roche Ltd, Pharmaceuticals Division, PRBD E, Building 065 512, 4070 Basel, Switzerland
    Anal Chem 78:4281-90. 2006
  8. pmc High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA
    Senada Nozinovic
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 38:683-94. 2010
  9. ncbi Comparison of multiple Amber force fields and development of improved protein backbone parameters
    Viktor Hornak
    Center for Structural Biology, Stony Brook University, Stony Brook, New York 11794, USA
    Proteins 65:712-25. 2006
  10. ncbi Linkage between dynamics and catalysis in a thermophilic-mesophilic enzyme pair
    Magnus Wolf-Watz
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Struct Mol Biol 11:945-9. 2004

Research Grants

Detail Information

Publications295 found, 100 shown here

  1. pmc BioMagResBank
    Eldon L Ulrich
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Nucleic Acids Res 36:D402-8. 2008
    ..protein.osaka-u.ac.jp) and the other at CERM, University of Florence, Florence, Italy (bmrb.postgenomicnmr.net/). The site at Osaka also accepts and processes data depositions...
  2. ncbi Evaluating protein structures determined by structural genomics consortia
    Aneerban Bhattacharya
    Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers University and Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Proteins 66:778-95. 2007
    ..Potential reasons for this "structure quality score gap" between NMR and X-ray crystal structures are discussed...
  3. ncbi The Xplor-NIH NMR molecular structure determination package
    Charles D Schwieters
    Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Building 12A, 20892 5624, Bethesda, MD, USA
    J Magn Reson 160:65-73. 2003
    ..Support for these scripting languages also facilitates interaction with existing external programs for structure analysis, structure manipulation, visualization, and spectral analysis...
  4. ncbi Structure of PTB bound to RNA: specific binding and implications for splicing regulation
    Florian C Oberstrass
    Institute for Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, Zurich, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Science 309:2054-7. 2005
    ..Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor...
  5. ncbi NMR characterization of the dynamics of biomacromolecules
    Arthur G Palmer
    Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Chem Rev 104:3623-40. 2004
  6. ncbi Combined chemical shift changes and amino acid specific chemical shift mapping of protein-protein interactions
    Frank H Schumann
    Institute of Biophysics and Physical Biochemistry, University of Regensburg, 93040 Regensburg, Germany
    J Biomol NMR 39:275-89. 2007
    ..As we show combined chemical shifts can also be applied for a more reliable quantitative evaluation of titration data...
  7. ncbi Probabilistic quotient normalization as robust method to account for dilution of complex biological mixtures. Application in 1H NMR metabonomics
    Frank Dieterle
    F Hoffman La Roche Ltd, Pharmaceuticals Division, PRBD E, Building 065 512, 4070 Basel, Switzerland
    Anal Chem 78:4281-90. 2006
    ....
  8. pmc High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA
    Senada Nozinovic
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 38:683-94. 2010
    ..Here, we discuss the impact of various NMR restraints on structure quality and discuss in detail the dynamics of this system as previously determined...
  9. ncbi Comparison of multiple Amber force fields and development of improved protein backbone parameters
    Viktor Hornak
    Center for Structural Biology, Stony Brook University, Stony Brook, New York 11794, USA
    Proteins 65:712-25. 2006
    ..It also accomplishes improved agreement with published experimental data for conformational preferences of short alanine peptides and better accord with experimental NMR relaxation data of test protein systems...
  10. ncbi Linkage between dynamics and catalysis in a thermophilic-mesophilic enzyme pair
    Magnus Wolf-Watz
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Struct Mol Biol 11:945-9. 2004
    ..This comparative and quantitative study of activity, structure and dynamics revealed a close link between protein dynamics and catalytic turnover...
  11. pmc Crystal structure of the APOBEC3G catalytic domain reveals potential oligomerization interfaces
    Shivender M D Shandilya
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Structure 18:28-38. 2010
    ..NMR solution data provides evidence that another interface, which coordinates a novel zinc site, also exists. Thus, the observed crystallographic interfaces of APOBEC3G may be important for both oligomerization and function...
  12. pmc Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model
    Francois Pierre J Martin
    Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, London, UK
    Mol Syst Biol 4:157. 2008
    ....
  13. ncbi Solution structure of the Reps1 EH domain and characterization of its binding to NPF target sequences
    S Kim
    Department of Biochemistry, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Biochemistry 40:6776-85. 2001
    ..In addition, we characterized binding to a peptide containing a DPF sequence (Kd = 0.5 +/- 0.2 mM). The characterization of binding between the Reps1 EH domain and its target proteins provides information about their role in endocytosis...
  14. ncbi NMR analysis of a 900K GroEL GroES complex
    Jocelyne Fiaux
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
    Nature 418:207-11. 2002
    ..This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes...
  15. ncbi Fast time scale dynamics of protein backbones: NMR relaxation methods, applications, and functional consequences
    Virginia A Jarymowycz
    Department of Chemistry and Interdisciplinary Biochemistry Program, Indiana University, Bloomington, Indiana 47405 0001, USA
    Chem Rev 106:1624-71. 2006
  16. ncbi Probing the conformational heterogeneity of the acetylaminofluorene-modified 2'-deoxyguanosine and DNA by 19F NMR spectroscopy
    B P Cho
    Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kingston 02881, USA
    Biochemistry 38:7572-83. 1999
    ..et al. (1997) J. Am. Chem. Soc. 119, 5384-5389]. The exclusive stacked nature of the AAF adducts may provide insight into why AAF adducts are more mutagenic and prone to repair than the nonacetylated AF adducts...
  17. pmc The NMR restraints grid at BMRB for 5,266 protein and nucleic acid PDB entries
    Jurgen F Doreleijers
    Radboud University Medical Centre Nijmegen, Geert Grooteplein 26 28, HB, Nijmegen, The Netherlands
    J Biomol NMR 45:389-96. 2009
    ..Continuous efforts in automating the parsing of both old, and newly deposited files is steadily decreasing this fraction. The cleaned data files are available from the NMR restraints grid at http://restraintsgrid.bmrb.wisc.edu ...
  18. ncbi Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts
    Olaf Beckonert
    Department of Biomolecular Medicine, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, UK
    Nat Protoc 2:2692-703. 2007
    ....
  19. doi Amyloid fibrils of the HET-s(218-289) prion form a beta solenoid with a triangular hydrophobic core
    Christian Wasmer
    Physical Chemistry, ETH Zurich, 8093 Zurich, Switzerland
    Science 319:1523-6. 2008
    ..The structure is likely to have broad implications for understanding the infectious amyloid state...
  20. ncbi Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3
    Hugo van Ingen
    Bijvoet Centre for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands
    Structure 16:1245-56. 2008
    ..Interference by asymmetric dimethylation of arginine 2 suggests that a H3R2/K4 "methyl-methyl" switch in the histone code dynamically regulates TFIID-promoter association...
  21. ncbi Correlation between the occurrence of 1H-MRS lipid signal, necrosis and lipid droplets during C6 rat glioma development
    Sonja Zoula
    Laboratoire mixte INSERM U438 Université Joseph Fourier, Laboratoire de Recherche Correspondant du CEA, Centre Hospitalier Universitaire, Grenoble, France
    NMR Biomed 16:199-212. 2003
    ..These necrotic areas contained large amounts of lipid droplets. All these results suggest that mobile lipids detected in vivo by 1H MRS (136 ms echo time) in C6 rat brain glioma arise mainly from lipid droplets located in necrosis...
  22. ncbi Structural basis of integrin activation by talin
    Kate L Wegener
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England, UK
    Cell 128:171-82. 2007
    ....
  23. pmc Inhibitors of amyloid toxicity based on beta-sheet packing of Abeta40 and Abeta42
    Takeshi Sato
    Department of Biochemistry and Cell Biology, Center for Structural Biology, Stony Brook University, Stony Brook, New York 11794 5215, USA
    Biochemistry 45:5503-16. 2006
    ..Importantly, the designed peptide inhibitors significantly reduce the toxicity induced by Abeta42 on cultured rat cortical neurons...
  24. ncbi PRIMe: a Web site that assembles tools for metabolomics and transcriptomics
    Kenji Akiyama
    RIKEN Plant Science Center, Yokohama, Kanagawa, Japan
    In Silico Biol 8:339-45. 2008
    ....
  25. ncbi A site-specific low-enrichment (15)N,(13)C isotope-labeling approach to unambiguous NMR spectral assignments in nucleic acids
    Anh Tuân Phan
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center New York, New York 10021, USA
    J Am Chem Soc 124:1160-1. 2002
    ..This simple and cost-effective methodology is demonstrated on the d(GGGTTCAGG) DNA sequence, which forms a dimeric G-quadruplex containing G.G.G.G tetrads sandwiched between G.(C-A) triads...
  26. pmc Metal-ion binding and metal-ion induced folding of the adenine-sensing riboswitch aptamer domain
    Jonas Noeske
    Department of Biochemistry, The University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA
    Nucleic Acids Res 35:5262-73. 2007
    ..Compared to the highly similar guanine-sensing riboswitch, the folding pathway for the adenine-sensing riboswitch aptamer domain is more complex and the influence of Mg2+ is more pronounced...
  27. pmc Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation
    Shivani Ahuja
    Department of Physics and Astronomy, Stony Brook University, Stony Brook, New York 11794 5215, USA
    Nat Struct Mol Biol 16:168-75. 2009
    ..These results provide a comprehensive view of how retinal isomerization triggers helix motion and activation in this prototypical G protein-coupled receptor...
  28. ncbi Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA
    Torsten Herrmann
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Honggerberg, CH 8093 Zurich, Switzerland
    J Mol Biol 319:209-27. 2002
    ....
  29. pmc NMR and biochemical characterization of recombinant human tRNA(Lys)3 expressed in Escherichia coli: identification of posttranscriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription
    C Tisne
    Laboratoire de Cristallographie et Résonance Magnétique Nucléaire Biologiques, Faculte de Pharmacie, Centre National de la Recherche Scientifique Equipe Postulante 2075, Paris, France
    RNA 6:1403-12. 2000
    ..coli (m2G10, psi27, m5C48, m5C49, and m1A58) as well as the mnm5 group at position 34 are dispensable for initiation of reverse transcription. In contrast, the 2-thio group at position 34 seems to play an important part in this process...
  30. ncbi Quantitative dynamics and binding studies of the 20S proteasome by NMR
    Remco Sprangers
    Department of Biochemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Nature 445:618-22. 2007
    ..Our results establish that NMR spectroscopy can provide detailed insight into supra-molecular structures over an order of magnitude larger than those routinely studied using methodology that is generally applicable...
  31. ncbi Structure of Bax: coregulation of dimer formation and intracellular localization
    M Suzuki
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 103:645-54. 2000
    ..The Bax structure shows that the orientation of helix alpha 9 provides simultaneous control over its mitochondrial targeting and dimer formation...
  32. ncbi Metabonomic and microbiological analysis of the dynamic effect of vancomycin-induced gut microbiota modification in the mouse
    Ivan K S Yap
    Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Proteome Res 7:3718-28. 2008
    ....
  33. pmc SCOR: a Structural Classification of RNA database
    Peter S Klosterman
    Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Nucleic Acids Res 30:392-4. 2002
    ..A classification of the well-characterized tertiary interactions found in the larger RNA structures is also included along with examples. The SCOR database is accessible at http://scor.lbl.gov...
  34. pmc Toward a unified representation of protein structural dynamics in solution
    Phineus R L Markwick
    Protein Dynamics and Flexibility, Institute de Biologie Structurale Jean Pierre Ebel, CNRS CEA UJF UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France
    J Am Chem Soc 131:16968-75. 2009
    ..This constraint-free approach thereby provides an atomic resolution free-energy weighted Boltzmann description of protein dynamics occurring on time scales over many orders of magnitude in the protein ubiquitin...
  35. doi Conformational averaging in structural biology: issues, challenges and computational solutions
    Daniela Kruschel
    Laboratory of Computational Biophysics, Mediterranean Institute for Life Sciences, Mestrovicevo setaliste bb, Split, HR 21000, Croatia
    Mol Biosyst 5:1606-16. 2009
    ....
  36. pmc Characterization of conformational and dynamic properties of natively unfolded human and mouse alpha-synuclein ensembles by NMR: implication for aggregation
    Kuen Phon Wu
    Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854, USA
    J Mol Biol 378:1104-15. 2008
    ..These transient interchain interactions coupled with a non-A beta amyloid component (NAC) region that is both more exposed and has a higher propensity to beta structure may accelerate the rate of fibril formation of aS...
  37. ncbi Complete protein structure determination using backbone residual dipolar couplings and sidechain rotamer prediction
    Michael Andrec
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, NJ 08854 8087, USA
    J Struct Funct Genomics 2:103-11. 2002
    ....
  38. ncbi A phase cycle scheme that significantly suppresses offset-dependent artifacts in the R2-CPMG 15N relaxation experiment
    Grover N B Yip
    Department of Chemistry, University of Michigan Biophysics Research Division 930 N University Ave, Ann Arbor, MI 48109, USA
    J Magn Reson 171:25-36. 2004
    ....
  39. pmc Probing the sequence effects on NarI-induced -2 frameshift mutagenesis by dynamic 19F NMR, UV, and CD spectroscopy
    Nidhi Jain
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA
    Biochemistry 46:13310-21. 2007
    ..We also provide evidence for AF/FAF conformational compatibility in the NarI sequences...
  40. ncbi Experimental and analytical variation in human urine in 1H NMR spectroscopy-based metabolic phenotyping studies
    Anthony D Maher
    Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics SORA, Faculty of Medicine, Imperial College London, South Kensington SW7 2AZ, United Kingdom
    Anal Chem 79:5204-11. 2007
    ....
  41. pmc Defining the role of active-site loop fluctuations in dihydrofolate reductase catalysis
    Dan McElheny
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:5032-7. 2005
    ....
  42. pmc Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence
    Jixun Dai
    College of Pharmacy, The University of Arizona, 1703 E Mabel St, Tucson, AZ 85721, USA
    Nucleic Acids Res 35:4927-40. 2007
    ....
  43. doi Solid-state NMR spectroscopy reveals that E. coli inclusion bodies of HET-s(218-289) are amyloids
    Christian Wasmer
    Laboratorium fur Physikalische Chemie, ETH Zurich, 8093 Zurich, Switzerland
    Angew Chem Int Ed Engl 48:4858-60. 2009
    ..coli IBs of the prion-forming domain from the fungal prion HET-s is the same as that previously determined for fibrils assembled in vitro, and show prion infectivity. These results demonstrate that the IBs of HET-s(218-289) are amyloids...
  44. ncbi 1H NMR metabonomics can differentiate the early atherogenic effect of dairy products in hyperlipidemic hamsters
    Jean Charles Martin
    Institut National de la Recherche Agronomique, UMR1260 Nutriments Lipidiques et Prevention des Maladies Metaboliques, Marseille, France
    Atherosclerosis 206:127-33. 2009
    ..This proof-of-principle study demonstrates the first use of plasma metabonomics for improving the prognosis of diet-induced atherogenesis, revealing novel potential disease biomarkers...
  45. pmc Constructing RNA dynamical ensembles by combining MD and motionally decoupled NMR RDCs: new insights into RNA dynamics and adaptive ligand recognition
    Aaron T Frank
    Department of Chemistry, University of California Irvine, 1102 Natural Sciences 2, Irvine, CA 92697, USA
    Nucleic Acids Res 37:3670-9. 2009
    ....
  46. ncbi Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA:RNA hybrids
    Michael Petersen
    Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Odense University, DK 5230 Odense M, Denmark
    J Am Chem Soc 124:5974-82. 2002
    ....
  47. pmc An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human BCL-2 promoter region in solution
    Jixun Dai
    College of Pharmacy, University of Arizona, Tucson, 85721, USA
    J Am Chem Soc 128:1096-8. 2006
    ..This predominant bcl-2 G-quadruplex represents an attractive novel target for the design of new anticancer drugs that specifically modulate bcl-2 gene expression...
  48. ncbi NMR structure of the full-length linear dimer of stem-loop-1 RNA in the HIV-1 dimer initiation site
    Nikolai B Ulyanov
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143, USA
    J Biol Chem 281:16168-77. 2006
    ..These guanines may be recognized by the nucleocapsid protein, which binds tightly to the G-bulge in vitro...
  49. pmc Characterization of the fast dynamics of protein amino acid side chains using NMR relaxation in solution
    Tatyana I Igumenova
    Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6059, USA
    Chem Rev 106:1672-99. 2006
  50. ncbi The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution
    F Mueller
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, Berlin, 14195, Germany
    J Mol Biol 298:35-59. 2000
    ....
  51. pmc Structural basis of site-specific histone recognition by the bromodomains of human coactivators PCAF and CBP/p300
    Lei Zeng
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York University, New York, NY 10029, USA
    Structure 16:643-52. 2008
    ....
  52. pmc Conformation of alamethicin in oriented phospholipid bilayers determined by (15)N solid-state nuclear magnetic resonance
    M Bak
    Laboratory for Biomolecular NMR Spectroscopy, Department of Molecular and Structural Biology, Science Park, University of Aarhus, DK-8000 Aarhus C, Denmark
    Biophys J 81:1684-98. 2001
    ..300:325-330, 1982). The solid-state NMR-compatible structures may support the formation of a left-handed and parallel multimeric ion channel...
  53. pmc A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III
    H Wu
    Department of Chemistry and Biochemistry, 405 Hilgard Avenue, PO Box 951569, University of California, Los Angeles, CA 90095-1569, USA
    EMBO J 20:7240-9. 2001
    ....
  54. pmc Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunit
    Geoffrey A Mueller
    Laboratory of Structural Biology, MR 01, National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 187:7081-9. 2005
    ..The new theta structure is expected to provide additional insight into its physiological role and its effect on the epsilon proofreading subunit...
  55. pmc Structure and mechanism of the M2 proton channel of influenza A virus
    Jason R Schnell
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 451:591-5. 2008
    ..Drug-resistance mutations are predicted to counter the effect of drug binding by either increasing the hydrophilicity of the pore or weakening helix-helix packing, thus facilitating channel opening...
  56. ncbi Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex
    Barbara Beuth
    Division of Molecular Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U K
    J Am Chem Soc 129:10205-10. 2007
    ..Our NMR binding assays using the third KH domain of the Nova-1 protein provide a proof-of-principle for the method and novel information on the specificity of this domain for its RNA targets...
  57. ncbi Competitive Na(+) and Rb(+) binding in the minor groove of DNA
    Flaminia Cesare Marincola
    Department of Biophysical Chemistry, Lund University, SE 22100 Lund, Sweden
    J Am Chem Soc 126:6739-50. 2004
    ..At 4 degrees C, the mean residence time of groove-bound ions is 0.2 +/- 0.1 micros for Rb(+) and 10 ns to 100 micros for Na(+). A shorter correlation time of 2 ns is attributed to counterions bridging cross-strand phosphate groups...
  58. ncbi A method for efficient isotopic labeling of recombinant proteins
    J Marley
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Biomol NMR 20:71-5. 2001
    ..Following a short period for growth recovery and unlabeled metabolite clearance, the cells are induced. The expression yields obtained provide a fourfold to eightfold reduction in isotope costs using simple shake flask growths...
  59. pmc The apical stem-loop of the hepatitis B virus encapsidation signal folds into a stable tri-loop with two underlying pyrimidine bulges
    Sara Flodell
    Department of Medical Biochemistry and Biophysics, Umea University, S 901 87 Umea, Sweden
    Nucleic Acids Res 30:4803-11. 2002
    ..In particular, the residues essential for this fold are either totally conserved or show rare non-disruptive mutations. These data strongly indicate that this fold is essential for recognition by the reverse transcriptase...
  60. ncbi Automated NMR structure calculation with CYANA
    Peter Güntert
    RIKEN Genomic Sciences Center, Yokohama, Japan
    Methods Mol Biol 278:353-78. 2004
    ....
  61. ncbi MUNIN: a new approach to multi-dimensional NMR spectra interpretation
    V Y Orekhov
    Swedish NMR Centre at Göteborg University
    J Biomol NMR 20:49-60. 2001
    ..As a result, the extraction of structural information simply consists of one-dimensional peak picking of the shape along the NOE-axis obtained for each amide group...
  62. ncbi Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel
    Hoi Jong Jung
    Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500 712, Korea
    Biochemistry 44:6015-23. 2005
    ....
  63. ncbi SOFAST-HMQC experiments for recording two-dimensional heteronuclear correlation spectra of proteins within a few seconds
    Paul Schanda
    Institut de Biologie Structurale Jean Pierre Ebel, UMR5075 CNRS CEA UJF, 41, rue Jules Horowitz, 38027, Grenoble Cedex, France
    J Biomol NMR 33:199-211. 2005
    ....
  64. pmc i-motif solution structure and dynamics of the d(AACCCC) and d(CCCCAA) tetrahymena telomeric repeats
    Narcisse Esmaili
    Laboratoire de RMN à Haut Champ, Institut de Chimie des Substances Naturelles Gif sur Yvette, F 91198, France
    Nucleic Acids Res 33:213-24. 2005
    ..In both topologies, the adenosine bases are fairly well stacked to the adjacent C*C+ pairs. They are not paired but form symmetrical pseudo-pairs with their H6cis amino proton and N1 nitrogen pointing towards each other...
  65. pmc Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses
    Denise S B Chan
    Department of Biochemistry, Centre for Protein Science and Crystallography and Molecular Biotechnology Programme, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    Nucleic Acids Res 35:1660-72. 2007
    ..In an analogy to the role of stalk proteins in binding elongation factors, we propose that interaction with acidic ribosomal stalk proteins help TCS to locate its RNA substrate...
  66. pmc HMDB: the Human Metabolome Database
    David S Wishart
    Department of Computing Science, University of Alberta, Edmonton, AB, Canada T6G 2E8
    Nucleic Acids Res 35:D521-6. 2007
    ..The HMDB is designed to address the broad needs of biochemists, clinical chemists, physicians, medical geneticists, nutritionists and members of the metabolomics community. The HMDB is available at: www.hmdb.ca...
  67. pmc The formation pathway of i-motif tetramers
    Jean Louis Leroy
    Laboratoire de Chimie et Biologie Structurale, Institut de Chimie des Substances Naturelles, Gif sur Yvette 91190, France
    Nucleic Acids Res 37:4127-34. 2009
    ....
  68. ncbi Conserved structure for single-stranded telomeric DNA recognition
    Rachel M Mitton-Fry
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    Science 296:145-7. 2002
    ..The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes...
  69. pmc How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures
    Robert Court
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO Rep 6:39-45. 2005
    ..The binding of the TRF2-Dbd to the DNA double helix shows no distortions that would account for the promotion of t-loops in which TRF2 has been implicated...
  70. doi [5mCCTCTCTCC]4: an i-motif tetramer with intercalated T*T pairs
    Muriel Canalia
    Laboratoire de Chimie et Biologie Structurale, Institut de Chimie des Substances Naturelles, Gif sur Yvette, France 91190
    J Am Chem Soc 131:12870-1. 2009
    ..This observation provides indications of the origin of the conflict that usually hinders T*T intercalation into i-motif structures and more generally of the constraints influencing i-motif formation...
  71. pmc Structural and dynamic characterization of an unfolded state of poplar apo-plastocyanin formed under nondenaturing conditions
    Y Bai
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Protein Sci 10:1056-66. 2001
    ....
  72. ncbi NMR structures of loop B RNAs from the stem-loop IV domain of the enterovirus internal ribosome entry site: a single C to U substitution drastically changes the shape and flexibility of RNA
    Zhihua Du
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    Biochemistry 43:5757-71. 2004
    ....
  73. doi Structure determination of protein-protein complexes using NMR chemical shifts: case of an endonuclease colicin-immunity protein complex
    Rinaldo W Montalvao
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Am Chem Soc 130:15990-6. 2008
    ..The results that we report thus indicate that chemical shifts can be used as structural restraints for the determination of the conformations of protein complexes that are difficult to obtain by more standard NMR approaches...
  74. pmc A comparative analysis of the equilibrium dynamics of a designed protein inferred from NMR, X-ray, and computations
    Lin Liu
    Department of Computational Biology, School of Medicine, University of Pittsburgh, Biomedical Science Tower 3, Pittsburgh, Pennsylvania 15213, USA
    Proteins 77:927-39. 2009
    ....
  75. ncbi Molecular structure of amyloid fibrils: insights from solid-state NMR
    Robert Tycko
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    Q Rev Biophys 39:1-55. 2006
    ....
  76. ncbi Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF
    Haitao Li
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 442:91-5. 2006
    ..Our findings call attention to the PHD finger as a previously uncharacterized chromatin-binding module found in a large number of chromatin-associated proteins...
  77. ncbi Recognition of pre-formed and flexible elements of an RNA stem-loop by nucleolin
    P Bouvet
    Laboratoire de Pharmacologie et de Biologie Structurale, 205 route de Narbonne, Toulouse Cedex, 31077, France
    J Mol Biol 309:763-75. 2001
    ..The loop E motif also has specific interactions with the protein. Implications of these findings for the mechanism of recognition of RNA structures by modular proteins are discussed...
  78. ncbi Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1)
    Thomas Strahl
    Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720 3202, USA
    J Biol Chem 282:30949-59. 2007
    ..Frq1 may activate Pik1 by facilitating membrane targeting via the exposed N-myristoyl group and by imposing a structural transition that promotes association of the lipid kinase unique motif with the kinase domain...
  79. ncbi EDTA-derivatized deoxythymidine as a tool for rapid determination of protein binding polarity to DNA by intermolecular paramagnetic relaxation enhancement
    Junji Iwahara
    Laboratories of Chemical Physics and Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Bethesda, MD 20892 0510, USA
    J Am Chem Soc 125:6634-5. 2003
    ....
  80. pmc Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III
    Haihong Wu
    Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, CA 90095 1569, USA
    Proc Natl Acad Sci U S A 101:8307-12. 2004
    ..These results show how a single dsRBD can convey specificity for particular RNA targets, by structure specific recognition of a conserved tetraloop fold...
  81. doi Characterization of enzyme motions by solution NMR relaxation dispersion
    J Patrick Loria
    Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA
    Acc Chem Res 41:214-21. 2008
    ..Solution NMR relaxation dispersion experiments are powerful experimental tools that can elucidate protein motions with atomic resolution and can provide insight into the role of these motions in biological function...
  82. pmc NMR structure of the active conformation of the Varkud satellite ribozyme cleavage site
    Bernd Hoffmann
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 100:7003-8. 2003
    ..The combination of NMR and bioinformatic approaches presented here has identified a novel RNA fold and provides insights into the structural basis of catalytic function in the Neurospora VS ribozyme...
  83. doi Differential responses of the backbone and side-chain conformational dynamics in FKBP12 upon binding the transition-state analog FK506: implications for transition-state stabilization and target protein recognition
    Ulrika Brath
    Division of Biophysical Chemistry, Center for Molecular Protein Science, Lund University, Lund, Sweden
    J Mol Biol 387:233-44. 2009
    ....
  84. ncbi Protein alignment by a coexpressed lanthanide-binding tag for the measurement of residual dipolar couplings
    Jens Wöhnert
    Institut für Organische Chemie und Chemische Biologie, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt M, Germany
    J Am Chem Soc 125:13338-9. 2003
    ..6 to 6.1 Hz for Dy3+, while an opposite alignment tensor is observed for Tm3+ (4.5 to -2.9 Hz) at 800 MHz. Experimental RDCs are in excellent agreement with those predicted on the basis of the X-ray structure of the protein...
  85. ncbi Using NMRView to visualize and analyze the NMR spectra of macromolecules
    Bruce A Johnson
    Molecular Systems, Merck Research Labs, Rahway, NJ, USA
    Methods Mol Biol 278:313-52. 2004
    ..NMR spectral peaks can be analyzed and assigned. A suite of tools exists within NMRView for assigning the data from triple-resonance experiments...
  86. ncbi NMR analysis of a Tau phosphorylation pattern
    Isabelle Landrieu
    CNRS UMR 8525, Institut Pasteur de Lille, 59019 Lille Cedex, France
    J Am Chem Soc 128:3575-83. 2006
    ..We finally demonstrate that the NMR approach can equally be used to evaluate potential kinase inhibitors in a straightforward manner...
  87. pmc A novel chair-type G-quadruplex formed by a Bombyx mori telomeric sequence
    Samir Amrane
    Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371
    Nucleic Acids Res 37:931-8. 2009
    ..Our result highlights the effect of G-tract length on the folding topology of G-quadruplexes, but also poses the question of whether a similar chair-type G-quadruplex fold exists in the human telomeric sequences...
  88. ncbi Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP
    D Liu
    Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Cell 94:573-83. 1998
    ..This protein mimicry of the TATA element surface provides the structural basis of the mechanism by which dTAF(II)230 negatively controls the TATA box-binding activity within the TFIID complex...
  89. ncbi Design of a novel globular protein fold with atomic-level accuracy
    Brian Kuhlman
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Science 302:1364-8. 2003
    ..2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature...
  90. pmc De novo protein structure generation from incomplete chemical shift assignments
    Yang Shen
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    J Biomol NMR 43:63-78. 2009
    ..This strategy, which uses traditional Rosetta for pre-filtering of the fragment selection process, is demonstrated for two paramagnetic proteins and also for two proteins with solid-state NMR chemical shift assignments...
  91. pmc Thermodynamics and kinetics for base-pair opening in the P1 duplex of the Tetrahymena group I ribozyme
    Joon Hwa Lee
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309 0215, USA
    Nucleic Acids Res 35:2965-74. 2007
    ....
  92. pmc Insight into the structural basis of pro- and antiapoptotic p53 modulation by ASPP proteins
    Jinwoo Ahn
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 284:13812-22. 2009
    ..Our detailed structural analyses of the ASPP-p53 interactions provide insight into the structural basis of the differential behavior of pro- and antiapoptotic ASPP family members...
  93. ncbi Discrepancies between the NMR and X-ray structures of uncomplexed barstar: analysis suggests that packing densities of protein structures determined by NMR are unreliable
    G S Ratnaparkhi
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore
    Biochemistry 37:6958-66. 1998
    ....
  94. pmc NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis
    Yuan Chao Lou
    Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
    Nucleic Acids Res 37:2381-94. 2009
    ..The tvMyb1(35-141)/DNA complex model furthers our understanding of DNA recognition by Myb proteins and this approach could be applied in determining the complex structures involving proteins with multiple domains...
  95. pmc NMR structure of the amino-terminal domain of the lambda integrase protein in complex with DNA: immobilization of a flexible tail facilitates beta-sheet recognition of the major groove
    Evgeny A Fadeev
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1570, USA
    J Mol Biol 388:682-90. 2009
    ..This finding reveals the structural basis for the observation that the "unstructured" amino terminus is required for recombination...
  96. pmc A correspondence between solution-state dynamics of an individual protein and the sequence and conformational diversity of its family
    Gregory D Friedland
    Graduate Group in Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS Comput Biol 5:e1000393. 2009
    ..More practically, the presented method can be applied to improve protein design predictions by accounting for intrinsic native-state dynamics...
  97. ncbi Protein backbone chemical shifts predicted from searching a database for torsion angle and sequence homology
    Yang Shen
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    J Biomol NMR 38:289-302. 2007
    ..52, 0.51, 0.27, 0.98, 1.07 and 1.08 ppm for (15)N, (1)H(N), (1)H(alpha), (13)C(alpha), (13)C(beta) and (13)C', respectively, including outliers...
  98. pmc Molecular basis for temperature sensing by an RNA thermometer
    Saheli Chowdhury
    Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, Switzerland
    EMBO J 25:2487-97. 2006
    ..Our results indicate that RNA thermometers are able to sense temperature changes without the aid of accessory factors...
  99. pmc Rapid preparation of RNA samples for NMR spectroscopy and X-ray crystallography
    Hae Kap Cheong
    Magnetic Resonance Team, Korea Basic Science Institute, Eoun Dong 52, Yuseong Gu, Daejeon 305 333, Korea
    Nucleic Acids Res 32:e84. 2004
    ..The 3' end tag is then removed by sequence-specific cleavage using trans-acting DNAzyme, the arm lengths of which are optimized for turnover number. This purification method is simpler and faster than the conventional method...
  100. ncbi On the effect of covalently appended quinolones on termini of DNA duplexes
    Jennifer Tuma
    Department of Chemistry, University of Constance, Fach M 709, D 78457 Konstanz, Germany
    J Am Chem Soc 124:4236-46. 2002
    ..The structure of the "molecular cap" with its disrupted terminal base pair may also be helpful for modeling how quinolones block re-ligation of DNA strands in the active site of gyrases...
  101. pmc Unusual intercalation of acridin-9-ylthiourea into the 5'-GA/TC DNA base step from the minor groove: implications for the covalent DNA adduct profile of a novel platinum-intercalator conjugate
    Hemanta Baruah
    Department of Chemistry, Wake Forest University, Winston Salem, NC 27109 7486, USA
    Nucleic Acids Res 31:4138-46. 2003
    ..The unusual tolerance of the GA/TC intercalation site and the pronounced groove specificity of ACRAMTU play a significant role in the molecular recognition between the corresponding platinum conjugate, Pt-ACRAMTU, and DNA...

Research Grants132 found, 100 shown here

  1. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2006
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  2. CORE--MACROMOLECULAR STRUCTURE
    Andrew Hinck; Fiscal Year: 2002
    ..The Shared Resource is directed by Andrew P. Hinck, Ph.D., who has worked in the field of biomolecular Nuclear Magnetic Resonance (NMR) for 14 years...
  3. CORE--NUCLEAR MAGNETIC RESONANCE FACILITY
    Stephen Prescott; Fiscal Year: 2004
    The Biomolecular Nuclear Magnetic Resonance (NMR) Shared Resource provides a state-of-the-art laboratory for biomolecular structure determination as well as routine analytical NMR services for the health sciences research community...
  4. NATIONAL BIOMEDICAL NMR RESOURCE AT MADISON
    John L Markley; Fiscal Year: 2011
    The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource for biomolecular nuclear magnetic resonance (NMR) spectroscopy...
  5. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2007
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs direct at improving human cancer treatments. ..
  6. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2005
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs directed at improving human cancer treatments. ..
  7. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2001
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs directed at improving human cancer treatments. ..
  8. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2002
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs directed at improving human cancer treatments. ..
  9. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2004
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs directed at improving human cancer treatments. ..
  10. Molecular Target Focused Discovery of Anticancer Drugs
    George R Pettit; Fiscal Year: 2010
    ....
  11. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2003
    ..In summary, the proposed research will be sharply aimed at the discovery and very rapid development of new anticancer drugs for the NCI programs directed at improving human cancer treatments. ..
  12. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2008
    ....
  13. Molecular Target Focused Discovery of Anticancer Drugs
    GEORGE PETTIT; Fiscal Year: 2009
    ....
  14. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2008
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  15. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2008
    ....
  16. Solid-state NMR studies of antimicrobial peptides
    Mei Hong; Fiscal Year: 2007
    ..Moreover, the proposed research will fill our knowledge gap of how beta-sheet peptides interact with lipid bilayers in general. ..
  17. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2007
    ..To achieve these aims, we will make use of state-of-the-art approaches to protein production and isotopic labeling, NMR data collection and analysis, modeling of structures and complexes, and quantum chemical calculations. ..
  18. Specialized Center for Eukaryotic Structural Genomics
    John Markley; Fiscal Year: 2008
    ..CESG will hold workshops and training programs. Activities of the Center will be monitored and guided by an advisory committee representing both biological and technical expertise. ..
  19. The sweet protein brazzein and its interaction with the human taste receptor
    Fariba Assadi Porter; Fiscal Year: 2009
    ..Explanation of molecular features that are essential for the brazzein-sweet receptor interaction may result in design of superior tasting non-caloric sweetener as an approach to help addressing diabetes and related disorders. ..
  20. Solid-state NMR studies of antimicrobial peptides
    Mei Hong; Fiscal Year: 2004
    ..Moreover, the proposed research will fill our knowledge gap of how beta-sheet peptides interact with lipid bilayers in general. ..
  21. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2007
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  22. PGIS, TXAS & PGES: STRUCTURE /FUNCTION
    Ke He Ruan; Fiscal Year: 2004
    ....
  23. 900 MHz NMR Spectrometer Biomolecular Investigations
    John Markley; Fiscal Year: 2006
    ..Web-based software will be used to pair users with local collaborator(s), assess specific needs, design and schedule data collection, and share results. ..
  24. The sweet protein brazzein and its interaction with the human taste receptor
    Fariba Assadi Porter; Fiscal Year: 2008
    ..Explanation of molecular features that are essential for the brazzein-sweet receptor interaction may result in design of superior tasting non-caloric sweetener as an approach to help addressing diabetes and related disorders. ..
  25. Solid-state NMR studies of antimicrobial peptides
    Mei Hong; Fiscal Year: 2006
    ..Moreover, the proposed research will fill our knowledge gap of how beta-sheet peptides interact with lipid bilayers in general. ..
  26. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2006
    ..Effort will also be expended toward developing new methodology for paramagnetic NMR, in particular, for systems that are not amenable to established approaches. ..
  27. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2006
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  28. Center for Eukaryotic Structural Genomics
    John Markley; Fiscal Year: 2004
    ..thaliana to be funded separately. The methods developed by the Center will be applicable to other eukaryotic genomes, including the human genome. ..
  29. Solid-state NMR of antimicrobial and cationic membrane peptides
    Mei Hong; Fiscal Year: 2008
    ..It will also benefit the development of more effective drug-delivery compounds that cross the cell membrane without damaging its integrity. ..
  30. PGIS, TXAS & PGES: STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 2005
    ....
  31. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2009
    ..To achieve these aims, we will make use of state-of-the-art approaches to protein production and isotopic labeling, NMR data collection and analysis, modeling of structures and complexes, and quantum chemical calculations. ..
  32. Solid-state NMR studies of antimicrobial peptides
    Mei Hong; Fiscal Year: 2005
    ..Moreover, the proposed research will fill our knowledge gap of how beta-sheet peptides interact with lipid bilayers in general. ..
  33. PGIS, TXAS & PGES: STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 2006
    ....
  34. Integrated state-of-the-art LC-MS/NMR system for the University of Wisconsin
    John Markley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  35. NATIONAL BIOMEDICAL NMR RESOURCE AT MADISON
    John Markley; Fiscal Year: 2007
    ..Our goal is to develop methods for making these investigations faster and less costly, as well as applicable to larger classes of proteins and nucleic acids of importance in human disease. ..
  36. PGI & TXA SYNTHASES-MEMBRANE ANCHOR STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 2001
    ....
  37. Solid-state NMR of antimicrobial and cationic membrane peptides
    Mei Hong; Fiscal Year: 2010
    ..It will also benefit the development of more effective drug-delivery compounds that cross the cell membrane without damaging its integrity. ..
  38. The sweet protein brazzein and its interaction with the human taste receptor
    Fariba M Assadi Porter; Fiscal Year: 2010
    ..Explanation of molecular features that are essential for the brazzein-sweet receptor interaction may result in design of superior tasting non-caloric sweetener as an approach to help addressing diabetes and related disorders. ..
  39. 900 MHZ NMR SPECTROMETER
    John Markley; Fiscal Year: 2001
    ..abstract_text> ..
  40. PGI & TXA SYNTHASES-MEMBRANE ANCHOR STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 2000
    ....
  41. NMR Spectrometer System Optimized for Direct 13C or 15N Detection
    John Markley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  42. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2000
    ....
  43. Solid-state NMR of antimicrobial and cationic membrane peptides
    Mei Hong; Fiscal Year: 2009
    ..It will also benefit the development of more effective drug-delivery compounds that cross the cell membrane without damaging its integrity. ..
  44. Solid-state NMR of influenza M2 protein in lipid bilayers
    Mei Hong; Fiscal Year: 2010
    ..The high-resolution structural information will be crucial for the design of new antiviral drugs to target the drug-resistant mutant proton channel, S31N-M2 in influenza A viruses. ..
  45. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 1999
    ....
  46. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John L Markley; Fiscal Year: 2010
    ..abstract_text> ..
  47. STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
    Ke He Ruan; Fiscal Year: 2007
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  48. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2007
    ....
  49. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2009
    ....
  50. CONFERENCE ON MAGNETIC RESONANCE IN BIOLOGICAL SYSTEMS
    John Markley; Fiscal Year: 2002
    ..Those receiving travel support will be selected by a committee composed of the U.S. members of the International Organizing Committee for the meeting. ..
  51. Solid-state NMR of influenza M2 protein in lipid bilayers
    Mei Hong; Fiscal Year: 2009
    ..The high-resolution structural information will be crucial for the design of new antiviral drugs to target the drug-resistant mutant proton channel, S31N-M2 in influenza A viruses. ..
  52. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2008
    ..To achieve these aims, we will make use of state-of-the-art approaches to protein production and isotopic labeling, NMR data collection and analysis, modeling of structures and complexes, and quantum chemical calculations. ..
  53. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2003
    ..Effort will also be expended toward developing new methodology for paramagnetic NMR, in particular, for systems that are not amenable to established approaches. ..
  54. Solid-state NMR studies of antimicrobial peptides
    Mei Hong; Fiscal Year: 2003
    ..Moreover, the proposed research will fill our knowledge gap of how beta-sheet peptides interact with lipid bilayers in general. ..
  55. PGIS, TXAS & PGES: STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 2006
    ....
  56. PGI & TXA SYNTHASES-MEMBRANE ANCHOR STRUCTURE/FUNCTION
    Ke He Ruan; Fiscal Year: 2002
    ....
  57. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2001
    ....
  58. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2007
    ..abstract_text> ..
  59. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John Markley; Fiscal Year: 2002
    ....
  60. NMR SPECTROSCOPY OF IRON-SULFUR PROTEINS
    John L Markley; Fiscal Year: 2010
    ..To achieve these aims, we will make use of state-of-the-art approaches to protein production and isotopic labeling, NMR data collection and analysis, modeling of structures and complexes, and quantum chemical calculations. ..
  61. Structural Basis of Dilated Cardiomyopathy
    Gianluigi Veglia; Fiscal Year: 2008
    ..To the best of our knowledge, these are the first biophysical studies of protein-protein interactions between integral membrane proteins and soluble proteins ever carried out using NMR spectroscopy. ..
  62. KALLIKREINS AS DIAGNOSTIC MARKERS OF OVARIAN CARCINOMA
    Eleftherios Diamandis; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  63. Structural Basis of Dilated Cardiomyopathy
    Gianluigi Veglia; Fiscal Year: 2007
    ..To the best of our knowledge, these are the first biophysical studies of protein-protein interactions between integral membrane proteins and soluble proteins ever carried out using NMR spectroseopy. ..
  64. Structural Basis of Dilated Cardiomyopathy
    Gianluigi Veglia; Fiscal Year: 2010
    ..To the best of our knowledge, these are the first biophysical studies of protein-protein interactions between integral membrane proteins and soluble proteins ever carried out using NMR spectroscopy. ..
  65. KALLIKREINS AS DIAGNOSTIC MARKERS OF OVARIAN CARCINOMA
    Eleftherios Diamandis; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  66. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2006
    ..abstract_text> ..
  67. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2005
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  68. Structural Basis of Dilated Cardiomyopathy
    Gianluigi Veglia; Fiscal Year: 2007
    ..To the best of our knowledge, these are the first biophysical studies of protein-protein interactions between integral membrane proteins and soluble proteins ever carried out using NMR spectroscopy. ..
  69. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2003
    ....
  70. Human Kallikrein 4 (hK4): A New Prostatic Biomarker?
    Eleftherios Diamandis; Fiscal Year: 2003
    ..If successful, our research will contribute to the development of new prostatic biomarkers and possibly, to better diagnosis which may lead to better clinical outcomes and reduced healthcare costs. ..
  71. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2002
    ....
  72. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2002
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  73. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2002
    ..abstract_text> ..
  74. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2002
    ....
  75. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2010
    ..Therefore, understanding SERCA regulation by both SLN and PLN is central to the development of non-conventional drug therapy such as gene therapy. ..
  76. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2000
    ....
  77. SYNTHESIS OF TEDANOLIDES CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 1999
    ....
  78. A New Biomarker for Ovarian Cancer Detection
    Eleftherios Diamandis; Fiscal Year: 2003
    ..In this grant proposal, we describe further studies, which will examine in greater detail, the ability of a test for the amount of this protein in blood to correctly diagnose the presence of ovarian cancer. ..
  79. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2003
    ..abstract_text> ..
  80. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2008
    ..Therefore, understanding SERCA regulation by both SLN and PLN is central to the development of non-conventional drug therapy such as gene therapy. ..
  81. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2005
    ..abstract_text> ..
  82. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2004
    ..abstract_text> ..
  83. KALLIKREINS AS DIAGNOSTIC MARKERS OF OVARIAN CARCINOMA
    Eleftherios Diamandis; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  84. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2003
    ..abstract_text> ..
  85. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2004
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  86. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2003
    ....
  87. NMR Structural Analysis of Calcium Regulation in Muscle
    Gianluigi Veglia; Fiscal Year: 2009
    ..Therefore, understanding SERCA regulation by both SLN and PLN is central to the development of non-conventional drug therapy such as gene therapy. ..
  88. Structural Basis of Dilated Cardiomyopathy
    Gianluigi Veglia; Fiscal Year: 2008
    ..To the best of our knowledge, these are the first biophysical studies of protein-protein interactions between integral membrane proteins and soluble proteins ever carried out using NMR spectroscopy. ..
  89. Structural Basis of Dilated Cardiomyopathy
    Gianluigi Veglia; Fiscal Year: 2009
    ..To the best of our knowledge, these are the first biophysical studies of protein-protein interactions between integral membrane proteins and soluble proteins ever carried out using NMR spectroscopy. ..
  90. SYNTHESIS OF THE TEDANOLIDES, CYTOTOXIC POLYPROPIONATES
    Michael Jung; Fiscal Year: 2001
    ....
  91. Structural Studies of Metabolic Membrane Proteins
    Joanne Yeh; Fiscal Year: 2003
    ..Our specific aims are to obtain atomic resolution structures of both membrane proteins, both of which are likely to be novel targets for antibiotic design and therapy. ..
  92. ANTIFUNGALS FROM MARINE INVERTEBRATES--AIDS ADJUVANTS
    TADEUSZ MOLINSKI; Fiscal Year: 2003
    ....
  93. Natural Product Antitumor Inducers of Apoptosis
    TADEUSZ MOLINSKI; Fiscal Year: 2003
    ..abstract_text> ..
  94. ANTIFUNGALS FROM MARINE INVERTEBRATES--AIDS ADJUVANTS
    TADEUSZ MOLINSKI; Fiscal Year: 2008
    ..Optimized leads identified from those libraries will be advanced to in vitro and in vivo trials. ..
  95. Nitroreductase: Determinants of Flavin Enzyme Activity
    Anne Frances Miller; Fiscal Year: 2003
    ..These tools will have widespread applicability to flavoproteins. ..
  96. Enzyme Mis-Metallation, Consequences and Opportunities
    Anne Frances Miller; Fiscal Year: 2009
    ..abstract_text> ..