fluorescence recovery after photobleaching

Summary

Summary: A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).

Top Publications

  1. ncbi Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope
    S Seiffert
    Institute of Physical Chemistry, Clausthal University of Technology, Clausthal Zellerfeld, Germany
    J Microsc 220:20-30. 2005
  2. ncbi Live-cell imaging reveals a stable cohesin-chromatin interaction after but not before DNA replication
    Daniel Gerlich
    Gene Expression Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Curr Biol 16:1571-8. 2006
  3. ncbi Investigating membrane protein dynamics in living cells
    Ian R Bates
    Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada
    Biochem Cell Biol 84:825-31. 2006
  4. ncbi Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells
    Satoru Kobayakawa
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Ten noudai, Tsukuba, Ibaraki 305 8572, Japan
    Genes Cells 12:447-60. 2007
  5. ncbi Development and use of fluorescent protein markers in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:87-91. 2003
  6. pmc Analysis of binding reactions by fluorescence recovery after photobleaching
    Brian L Sprague
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 86:3473-95. 2004
  7. pmc Mobility of cytoplasmic, membrane, and DNA-binding proteins in Escherichia coli
    Mohit Kumar
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Heidelberg, Germany
    Biophys J 98:552-9. 2010
  8. ncbi Polyglutamine protein aggregates are dynamic
    Soojin Kim
    Department of Biochemistry, Molecular Biology and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Nat Cell Biol 4:826-31. 2002
  9. pmc FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?
    Florian Mueller
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 22:403-11. 2010
  10. ncbi Dynamics of single mRNPs in nuclei of living cells
    Yaron Shav-Tal
    Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 304:1797-800. 2004

Research Grants

  1. MECHANICAL LOADING AND BONE
    Hiroki Yokota; Fiscal Year: 2007
  2. Function and Expression of Connexins in the pre-Botzinger Complex
    JONATHAN KELTY; Fiscal Year: 2007
  3. THE ROLES OF ESPINS IN HAIR CELL STEREOCILIA
    JAMES BARTLES; Fiscal Year: 2007
  4. MECHANICAL LOADING AND BONE
    Hiroki Yokota; Fiscal Year: 2010
  5. INTERMEDIATE FILAMENT--CELL SURFACE INTERACTIONS
    Robert Goldman; Fiscal Year: 1991
  6. Analysis of Z-band assembly and maintenance in living skeletal muscle cells
    JOSEPH WILLIAM contact SANGER; Fiscal Year: 2010
  7. Novel Epigenetic Marks in Mitosis
    KENNETH S contact ZARET; Fiscal Year: 2010
  8. Control of Vascular Cell Motility by CaMKII
    Harold A Singer; Fiscal Year: 2010
  9. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    DONALD BENEDICT DEFRANCO; Fiscal Year: 2010
  10. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    DONALD BENEDICT DEFRANCO; Fiscal Year: 2010

Detail Information

Publications291 found, 100 shown here

  1. ncbi Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope
    S Seiffert
    Institute of Physical Chemistry, Clausthal University of Technology, Clausthal Zellerfeld, Germany
    J Microsc 220:20-30. 2005
    ..of the diffusion process can be determined by straightforward and facile data analysis, when fluorescence recovery after photobleaching (FRAP) is measured as a function of time and space by means of confocal laser scanning ..
  2. ncbi Live-cell imaging reveals a stable cohesin-chromatin interaction after but not before DNA replication
    Daniel Gerlich
    Gene Expression Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Curr Biol 16:1571-8. 2006
    ....
  3. ncbi Investigating membrane protein dynamics in living cells
    Ian R Bates
    Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada
    Biochem Cell Biol 84:825-31. 2006
    ..of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking...
  4. ncbi Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells
    Satoru Kobayakawa
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Ten noudai, Tsukuba, Ibaraki 305 8572, Japan
    Genes Cells 12:447-60. 2007
    ..Thus, this experimental system should facilitate studies focusing on relationships between nuclear organization, epigenetic status and cell differentiation...
  5. ncbi Development and use of fluorescent protein markers in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:87-91. 2003
    ....
  6. pmc Analysis of binding reactions by fluorescence recovery after photobleaching
    Brian L Sprague
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 86:3473-95. 2004
    b>Fluorescence recovery after photobleaching (FRAP) is now widely used to investigate binding interactions in live cells...
  7. pmc Mobility of cytoplasmic, membrane, and DNA-binding proteins in Escherichia coli
    Mohit Kumar
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Heidelberg, Germany
    Biophys J 98:552-9. 2010
    ..Here we combined fluorescence recovery after photobleaching with numerical modeling to analyze mobility of a set of fluorescent protein fusions in the ..
  8. ncbi Polyglutamine protein aggregates are dynamic
    Soojin Kim
    Department of Biochemistry, Molecular Biology and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Nat Cell Biol 4:826-31. 2002
    ..These studies provide new insights into the composite organization and formation of protein aggregates and show that molecular chaperones are not sequestered into aggregates, but are instead transiently associated...
  9. pmc FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?
    Florian Mueller
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 22:403-11. 2010
    ..in live cells has been analyzed by kinetic modeling procedures applied to experimental data from fluorescence recovery after photobleaching (FRAP)...
  10. ncbi Dynamics of single mRNPs in nuclei of living cells
    Yaron Shav-Tal
    Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 304:1797-800. 2004
    ..This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model...
  11. pmc Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates
    Timothy J Stasevich
    National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 99:3093-101. 2010
    ..To address this uncertainty, we compare fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) measurements of the binding kinetics of a ..
  12. pmc Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes
    Diana A Stavreva
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute Light Imaging Facility, National Institute for Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA
    Mol Cell Biol 24:2682-97. 2004
    ..Moreover, our results reveal that longer GR residence times are consistently associated with greater transcriptional output, suggesting a new paradigm in which the rate of rapid exchange provides a means to tune transcriptional levels...
  13. pmc Protein diffusion in mammalian cell cytoplasm
    Thomas Kuhn
    Nanoscience Center, Department of Physics, University of Jyvaskyla, Jyvaskyla, Finland
    PLoS ONE 6:e22962. 2011
    ..The cytosol results were found to be in very good agreement with those by FCS...
  14. pmc Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching
    Jérôme Bove
    Departement de sciences biologiques, Universite de Montreal, Montreal, Quebec, Canada H1X 2B2
    Plant Physiol 147:1646-58. 2008
    ..b>Fluorescence recovery after photobleaching confirmed vesicle accumulation in the shoulder of the apex, and it revealed that the extreme ..
  15. pmc Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching
    Florian Mueller
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, Maryland 20892, USA
    Biophys J 94:3323-39. 2008
    ..transcription factors with chromatin have been investigated recently using quantitative fluorescence recovery after photobleaching (FRAP)...
  16. ncbi Effects of interphase and mitotic phosphorylation on the mobility and location of nucleolar protein B23
    Sandeep S Negi
    Department of Biochemistry, University of Mississippi Medical Center, Jackson, MS 39216, USA
    J Cell Sci 119:3676-85. 2006
    ..results in slower recovery of the mutant compared with the wild-type protein as measured by fluorescence recovery after photobleaching (FRAP)...
  17. pmc Intrinsic dynamic behavior of fascin in filopodia
    YVONNE S ARATYN
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Mol Biol Cell 18:3928-40. 2007
    ..b>Fluorescence recovery after photobleaching analysis revealed that fascin rapidly dissociates from filopodial filaments with a kinetic off-..
  18. pmc Kinetic and molecular analysis of nuclear export factor CRM1 association with its cargo in vivo
    Dirk Daelemans
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Mol Cell Biol 25:728-39. 2005
    ..Using fluorescence recovery after photobleaching (FRAP), we show that CRM1 moves at rates similar to that of free green fluorescent protein in ..
  19. pmc Dosage-sensitive regulation of cohesin chromosome binding and dynamics by Nipped-B, Pds5, and Wapl
    Maria Gause
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St Louis, MO 63104, USA
    Mol Cell Biol 30:4940-51. 2010
    ..We addressed this question by in vivo fluorescence recovery after photobleaching (FRAP) with Drosophila salivary glands...
  20. pmc Endocytosis is required for E-cadherin redistribution at mature adherens junctions
    Simon de Beco
    Centre de Recherche, Institut Curie, F 75248 Paris, France
    Proc Natl Acad Sci U S A 106:7010-5. 2009
    ..the dynamics of E-cadherin were quantitatively analyzed by a new approach combining 2-photon fluorescence recovery after photobleaching (FRAP) and fast 3D wide-field fluorescence microscopy...
  21. pmc Ras diffusion is sensitive to plasma membrane viscosity
    J Shawn Goodwin
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biophys J 89:1398-410. 2005
    ..Using confocal fluorescence recovery after photobleaching, we examined the diffusion of green fluorescent protein (GFP)-tagged HRas, NRas, and KRas in COS-..
  22. doi Conditions for using FRAP as a quantitative technique--influence of the bleaching protocol
    Dominika O Trembecka
    Faculty of Biochemistry, Biophysics and Biotechnology, Division of Cell Biophysics, Jagiellonian University, Krakow, Poland
    Cytometry A 77:366-70. 2010
    b>Fluorescence recovery after photobleaching (FRAP) is a tool widely used in studies of dynamic behavior of fluorescently-tagged proteins in live cells...
  23. pmc Differentiation driven changes in the dynamic organization of Basal transcription initiation
    Giuseppina Giglia-Mari
    Department of Genetics, Erasmus MC, Rotterdam, The Netherlands
    PLoS Biol 7:e1000220. 2009
    ....
  24. pmc Ligand-specific dynamics of the progesterone receptor in living cells and during chromatin remodeling in vitro
    Geetha V Rayasam
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, National Cancer Institute, National Institutes of Health, 41 Library Dr, Bethesda, MD 20892 5055, USA
    Mol Cell Biol 25:2406-18. 2005
    ..of the interaction of PR with a natural target promoter in living cells through the use of fluorescence recovery after photobleaching (FRAP) analysis and also have characterized the dynamics of the interaction of PR with the mouse ..
  25. pmc Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis
    Timothy J Stasevich
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, US National Institutes of Health, Bethesda, MD 20892 5055, USA
    EMBO J 29:1225-34. 2010
    ..Using a novel fluorescence recovery after photobleaching (FRAP) procedure, we have measured the affinities of these regions individually, in pairs, and ..
  26. doi How to quantify protein diffusion in the bacterial membrane
    Siet M J L van den Wildenberg
    Department of Physics and Astronomy, VU University, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands
    Biopolymers 95:312-21. 2011
    ..using various experimental techniques, including fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), and particle tracking using single-molecule fluorescence (SMF) microscopy...
  27. pmc A quantitative approach to analyze binding diffusion kinetics by confocal FRAP
    Minchul Kang
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Biophys J 99:2737-47. 2010
    ..We validate this method by measuring kinetic rate constants for the CAAX-mediated binding of Ras to membranes of the endoplasmic reticulum, obtaining binding constants of k(on) ∼ 255/s and k(off) ∼ 31/s...
  28. pmc Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase
    Yoko Hayashi-Takanaka
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    J Cell Biol 187:781-90. 2009
    ..Visualizing histone modifications in living cells will facilitate future epigenetic and cell regulation studies...
  29. pmc Temporal changes in microvessel leakiness during wound healing discriminated by in vivo fluorescence recovery after photobleaching
    Maria J C Machado
    Centre for Molecular Biosciences, University of Ulster, Cromore Road, Coleraine, Co Londonderry, UK
    J Physiol 589:4681-96. 2011
    ..vessels, angiogenic plexus and blind-ended vessels (BEVs)) was quantified using in vivo fluorescence recovery after photobleaching (FRAP) and linear regression analysis of recovery profiles...
  30. pmc The human deafness-associated connexin 30 T5M mutation causes mild hearing loss and reduces biochemical coupling among cochlear non-sensory cells in knock-in mice
    Melanie Schütz
    Institut fuer Genetik, Rheinische Friedrich Wilhelms Universitaet Bonn, Roemerstrasse 164, D 53117 Bonn, Germany
    Hum Mol Genet 19:4759-73. 2010
    ..Our findings link hearing loss to decreased biochemical coupling due to the point-mutated Cx30 in mice...
  31. pmc The transcriptional cycle of HIV-1 in real-time and live cells
    Stephanie Boireau
    Institute of Molecular Genetics of Montpellier, Unite Mixte de Recherche 5535, Centre National de la Recherche Scientifique, 34293 Montpellier, France
    J Cell Biol 179:291-304. 2007
    ..The strengths and limitations of this kinetic approach to analyze mRNA biogenesis in living cells are discussed...
  32. doi Caveolin-1 loss of function accelerates glucose transporter 4 and insulin receptor degradation in 3T3-L1 adipocytes
    Elena González-Muñoz
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Institute for Research in Biomedicine IRB Barcelona, Serveis Científico Tècnics, Universitat de Barcelona, 08028 Barcelona, Spain
    Endocrinology 150:3493-502. 2009
    ..We propose that caveolin-1/caveolae control insulin action in adipose cells...
  33. doi Gephyrin oligomerization controls GlyR mobility and synaptic clustering
    Martino Calamai
    INSERM U789 Biologie Cellulaire de Synapse and Département de Biologie, Ecole Normale Superieure, 75005 Paris, France
    J Neurosci 29:7639-48. 2009
    ..Using fluorescence recovery after photobleaching, we studied the exchange kinetics of synaptic gephyrin clusters...
  34. pmc Phase coexistence and connectivity in the apical membrane of polarized epithelial cells
    Doris Meder
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 103:329-34. 2006
    ..connectivity was assessed by measuring long-range diffusion of several membrane proteins by fluorescence recovery after photobleaching in two polarized epithelial cell lines and one fibroblast cell line...
  35. pmc Obstructed diffusion in phase-separated supported lipid bilayers: a combined atomic force microscopy and fluorescence recovery after photobleaching approach
    Timothy V Ratto
    Biophysics Graduate Group, Division of Biological Sciences, University of California Davis, One Shields Avenue, Davis, CA 95616, USA
    Biophys J 83:3380-92. 2002
    ..b>Fluorescence recovery after photobleaching was used to measure the lateral diffusion coefficient in single supported bilayers composed of ..
  36. pmc Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin
    Georgia Xouri
    Laboratory of General Biology, School of Medicine, University of Patras, Patras, Greece
    EMBO J 26:1303-14. 2007
    ..We propose that dynamic Cdt1-chromatin associations and the recruitment of Geminin to chromatin provide spatio-temporal control of the licensing process...
  37. pmc Stress fibers are generated by two distinct actin assembly mechanisms in motile cells
    Pirta Hotulainen
    Institute of Biotechnology, University of Helsinki, Helsinki FI 00014, Finland
    J Cell Biol 173:383-94. 2006
    ..b>Fluorescence recovery after photobleaching analysis revealed that actin filament cross-linking in stress fibers is highly dynamic, ..
  38. pmc Myofibrillogenesis in skeletal muscle cells in zebrafish
    Joseph W Sanger
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York, USA
    Cell Motil Cytoskeleton 66:556-66. 2009
    ..b>Fluorescence Recovery After Photobleaching showed that the exchange of proteins (actin, alpha-actinin, FATZ, myotilin and telethonin) ..
  39. ncbi Fluorescence recovery after photobleaching studies of lipid rafts
    Anne K Kenworthy
    Dept of Molecular Physiology and Biophysics, Vanderbilt School of Medicine, Nashville, TN 37232, USA
    Methods Mol Biol 398:179-92. 2007
    b>Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique that can be used to ask how lipid rafts impact protein and lipid diffusion in cells...
  40. ncbi Membrane mobility and microdomain association of the dopamine transporter studied with fluorescence correlation spectroscopy and fluorescence recovery after photobleaching
    Erika M Adkins
    Molecular Neuropharmacology Group, Department of Neuroscience and Pharmacology and Center for Pharmacogenomics, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Biochemistry 46:10484-97. 2007
    ..dopamine transporter (DAT), we employed FCS (fluorescence correlation spectroscopy) and FRAP (fluorescence recovery after photobleaching)...
  41. pmc Modeling fluorescence recovery after photobleaching in loaded bone: potential applications in measuring fluid and solute transport in the osteocytic lacunar-canalicular system
    Xiaozhou Zhou
    Department of Mechanical Engineering, Center for Biomedical Engineering Research, University of Delaware, 126 Spencer Laboratory, Newark, DE 19716, USA
    Ann Biomed Eng 36:1961-77. 2008
    ..system is essential for osteocyte viability and function, and it can be measured using fluorescence recovery after photobleaching (FRAP)...
  42. ncbi Knee-loading modality drives molecular transport in mouse femur
    Min Su
    Department of Anatomy and Cell Biology, Indiana University Purdue University, Indianapolis, 46202, USA
    Ann Biomed Eng 34:1600-6. 2006
    ..order to evaluate load-driven molecular transport in a lacunocanalicular network, we conducted fluorescence recovery after photobleaching (FRAP) experiments using lacunae stained with uranine (376 Da)...
  43. ncbi Measurement of diffusion within the cell wall in living roots of Arabidopsis thaliana
    Eric M Kramer
    Biology Department, University of Massachusetts, 611 N Pleasant St, Amherst, MA 01003, USA
    J Exp Bot 58:3005-15. 2007
    ..The diffusion coefficient, D(cw), of CF in the cell wall was probed using two protocols: fluorescence recovery after photobleaching and fluorescence loss following perfusion with dye-free solution...
  44. doi Fibrillarin and Nop56 interact before being co-assembled in box C/D snoRNPs
    Tanguy Lechertier
    Institut Jacques Monod, UMR 7592 CNRS Universités Paris 6 et 7, 2 place Jussieu, 75251 Paris Cedex 05, France
    Exp Cell Res 315:928-42. 2009
    ..In addition, no RNA seems required to maintain fibrillarin-Nop56 interaction...
  45. doi Accurate quantification of diffusion and binding kinetics of non-integral membrane proteins by FRAP
    Ronen Berkovich
    School of Chemistry, Raymond and Beverley Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Traffic 12:1648-57. 2011
    ..Despite a fair number of approximate fitting functions for analyzing fluorescence recovery after photobleaching (FRAP) data, no approach was able to cope with the full diffusion-exchange problem...
  46. doi Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching
    Florian Mueller
    Groupe Imagerie et Modélisation, Institut Pasteur, CNRS, URA 2582, Paris, France
    Methods Mol Biol 833:153-76. 2012
    b>Fluorescence recovery after photobleaching (FRAP) has now become widely used to investigate nuclear protein binding to chromatin in live cells...
  47. pmc Uptake, distribution and diffusivity of reactive fluorophores in cells: implications toward target identification
    Christopher W Cunningham
    Department of Medicinal Chemistry and Pharmaceutical Chemistry, Molecular Graphics and Modeling Laboratory, and Specialized Chemistry Center, University of Kansas, Lawrence, Kansas, USA
    Mol Pharm 7:1301-10. 2010
    ..The intracellular diffusion coefficients were measured using a fluorescence recovery after photobleaching (FRAP)-based method...
  48. pmc In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160
    Stefan Wagner
    Dept of Cell Biology, S7 214, University of Massachusetts Medical School, 55 Lake Ave, Worcester, MA 01655, USA
    J Cell Biol 164:843-50. 2004
    ..binding and mobility of enhanced green fluorescent protein (EGFP)-labeled nuclear proteins by fluorescence recovery after photobleaching in digitonin-permeabilized cells...
  49. pmc Are assumptions about the model type necessary in reaction-diffusion modeling? A FRAP application
    Juliane Mai
    Department of Computational Hydrosystems, UFZ Helmholtz Centre for Environmental Research, Leipzig, Germany
    Biophys J 100:1178-88. 2011
    At present, fluorescence recovery after photobleaching (FRAP) data are interpreted using various types of reaction-diffusion (RD) models: the model type is usually fixed first, and corresponding model parameters are inferred subsequently...
  50. pmc Fluorescence recovery after photobleaching and photoconversion in multiple arbitrary regions of interest using a programmable array microscope
    Guy M Hagen
    Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Microsc Res Tech 72:431-40. 2009
    ..b>Fluorescence recovery after photobleaching (FRAP) is commonly used for the determination of lateral diffusion constants of membrane ..
  51. doi Fully automated attenuation measurement and motion correction in FLIP image sequences
    Martijn van de Giessen
    Division of Image Processing LKEB, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    IEEE Trans Med Imaging 31:461-73. 2012
    ..The proposed method is fully automatic, and runs in approximately one minute per sequence, making it suitable for unsupervised batch processing of large data series...
  52. pmc A generalization of theory for two-dimensional fluorescence recovery after photobleaching applicable to confocal laser scanning microscopes
    Minchul Kang
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Biophys J 97:1501-11. 2009
    b>Fluorescence recovery after photobleaching (FRAP) using confocal laser scanning microscopes (confocal FRAP) has become a valuable technique for studying the diffusion of biomolecules in cells...
  53. ncbi Role of three-dimensional bleach distribution in confocal and two-photon fluorescence recovery after photobleaching experiments
    Davide Mazza
    Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy MicroSCoBiO Research Center, Department of Physics, University of Genoa, Italy
    Appl Opt 46:7401-11. 2007
    The quantitative analysis of fluorescence perturbation experiments such as fluorescence recovery after photobleaching (FRAP) requires suitable analytical models to be developed...
  54. ncbi Mobility of model proteins in hydrogels composed of oppositely charged dextran microspheres studied by protein release and fluorescence recovery after photobleaching
    Sophie R Van Tomme
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, University Utrecht, The Netherlands
    J Control Release 110:67-78. 2005
    ..These results show that these hydrogels are very suitable as injectable matrix for diffusion-controlled delivery of pharmaceutically active proteins...
  55. doi FRAP analysis of molecular diffusion inside sea-urchin spermatozoa
    Daisuke Takao
    Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro, Tokyo 153 8902, Japan
    J Exp Biol 211:3594-600. 2008
    ..Using a FRAP (fluorescence recovery after photobleaching) technique, we determined the diffusion coefficients of fluorescein-derivatives (calcein, ..
  56. pmc A closed-form analytic expression for FRAP formula for the binding diffusion model
    Minchul Kang
    Biophys J 95:L13-5. 2008
    ..b>Fluorescence recovery after photobleaching (FRAP) can be used to determine these kinetic constants in living cells...
  57. pmc Dominant-negative mechanism of leukemogenic PAX5 fusions
    N Kawamata
    Hematology Oncology, Cedars Sinai Medical Center UCLA School of Medicine, Los Angeles, CA, USA
    Oncogene 31:966-77. 2012
    ..PAX5-C20S is a tetramer, which interacts extraordinarily stably with chromatin as determined by Fluorescence Recovery After Photobleaching in living cells...
  58. doi Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk
    Nick Smisdom
    Hasselt University, Transnational University Limburg, Biomedical Research Institute, School of Life Sciences, Agoralaan Building C, Diepenbeek 3590, Belgium
    J Biomed Opt 16:046021. 2011
    b>Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam...
  59. pmc Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells
    Jacek T Mika
    Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, Netherlands Proteomics Centre and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
    PLoS ONE 6:e25664. 2011
    Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP)...
  60. pmc Fatty acid 2-hydroxylase mediates diffusional mobility of Raft-associated lipids, GLUT4 level, and lipogenesis in 3T3-L1 adipocytes
    Lin Guo
    Department of Internal Medicine, Center for Human Nutrition, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 285:25438-47. 2010
    ..of FA2H appeared to increase raft lipid mobility as it significantly accelerated the rates of fluorescence recovery after photobleaching measurements of lipid rafts labeled with Alexa 488-conjugated cholera toxin subunit B...
  61. pmc Dynamics of the alpha6beta4 integrin in keratinocytes
    Cecile A W Geuijen
    Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Mol Biol Cell 13:3845-58. 2002
    ..This clustering ultimately determines whether alpha6beta4 will inhibit cell migration or not...
  62. pmc Quantitative kinetic study of the actin-bundling protein L-plastin and of its impact on actin turn-over
    Ziad Al Tanoury
    Laboratory of Cytoskeleton and Cell Plasticity, Life Sciences Research Unit, University of Luxembourg, Luxembourg City, Luxembourg
    PLoS ONE 5:e9210. 2010
    ..Phosphorylation of L-plastin on residue Ser5 increases its F-actin binding activity and is required for L-plastin-mediated cell invasion...
  63. ncbi Gap junctional intercellular communication capacity by gap-FRAP technique: a comparative study
    Muriel Abbaci
    Faculte de Medecine, Nancy University, Vandoeuvre les Nancy, France
    Biotechnol J 2:50-61. 2007
    ..in monolayer cultures, characterized by different patterns of phosphorylated Cx43, we used a fluorescence recovery after photobleaching (FRAP) technique, and compared two tracers, 5(6)-carboxyfluorescein diacetate (CFDA) and calcein ..
  64. doi Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli
    Kristin M Slade
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Biochemistry 48:5083-9. 2009
    b>Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding ..
  65. pmc FRAP analysis of membrane-associated proteins: lateral diffusion and membrane-cytoplasmic exchange
    Nathan W Goehring
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Biophys J 99:2443-52. 2010
    Obtaining quantitative kinetic parameters from fluorescence recovery after photobleaching (FRAP) experiments generally requires a theoretical analysis of protein mobility and appropriate solutions for FRAP recovery derived for a given ..
  66. ncbi Intranuclear ataxin1 inclusions contain both fast- and slow-exchanging components
    David L Stenoien
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Nat Cell Biol 4:806-10. 2002
    ..Here, we used fluorescence recovery after photobleaching (FRAP) to examine the intranuclear dynamics of polyglutamine-expanded ataxin1 and inclusion-..
  67. ncbi Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC
    Martijn S Luijsterburg
    Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, Nuclear Organisation Group, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
    J Cell Sci 120:2706-16. 2007
    ..We propose a scenario in which DDB2 prepares UV-damaged chromatin for assembly of the NER complex...
  68. ncbi The interaction between Stargazin and PSD-95 regulates AMPA receptor surface trafficking
    Cecile Bats
    Physiologie Cellulaire de la Synapse, UMR 5091 CNRS Institut François Magendie, Universite Bordeaux, Bordeaux 33077, France
    Neuron 53:719-34. 2007
    ..These results propose a model in which the Stargazin-PSD-95 interaction plays a key role to trap and transiently stabilize diffusing AMPARs in the postsynaptic density...
  69. pmc Investigating complexity of protein-protein interactions in focal adhesions
    Tanmay P Lele
    Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA
    Biochem Biophys Res Commun 369:929-34. 2008
    ..Here, we used the fluorescence recovery after photobleaching (FRAP) technique, combined with mathematical modeling and scaling analysis to quantify ..
  70. pmc Modulation of RNA polymerase assembly dynamics in transcriptional regulation
    Stanislaw A Gorski
    National Cancer Institute, Laboratory of Receptor Biology and Gene Expression, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 30:486-97. 2008
    ..Our results show that regulation of rDNA transcription in vivo occurs via modulation of the efficiency of transcription complex subunit capture and assembly...
  71. pmc Sorting of lipids and proteins in membrane curvature gradients
    A Tian
    Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 96:2676-88. 2009
    ..The sorting of Cholera toxin subunit B is rationalized by statistical models. We discuss the implications of our findings for intracellular sorting mechanisms...
  72. pmc Human ISWI chromatin-remodeling complexes sample nucleosomes via transient binding reactions and become immobilized at active sites
    Fabian Erdel
    Research Group Genome Organization and Function, Deutsches Krebsforschungszentrum and BIOQUANT, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 107:19873-8. 2010
    ..Due to the relatively high intranuclear remodeler concentrations cellular response times for repositioning a given nucleosome were calculated to be in the range of tens of seconds to minutes...
  73. ncbi Modulation of heterochromatin protein 1 dynamics in primary Mammalian cells
    Richard Festenstein
    CSC Gene Control Mechanisms and Disease Group, Division of Medicine, Imperial College School of Medicine, Hammersmith Campus, Du Cane Road, London W12 ONN, UK
    Science 299:719-21. 2003
    ..We demonstrate here, by fluorescence recovery after photobleaching, that a green fluorescent protein-HP1beta fusion protein is highly mobile within both the ..
  74. pmc In situ measurement of solute transport in the bone lacunar-canalicular system
    Liyun Wang
    Leni and Peter W May Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 102:11911-6. 2005
    ..By using fluorescence recovery after photobleaching, the movement of a vitally injected fluorescent dye (sodium fluorescein) among individual ..
  75. pmc Analyzing intracellular binding and diffusion with continuous fluorescence photobleaching
    Malte Wachsmuth
    Division Biophysics of Macromolecules, German Cancer Research Center, D 69120 Heidelberg, Germany
    Biophys J 84:3353-63. 2003
    ..The cells show fluorescent nucleoli, and binding is transient. CP yields residence times for mTTF-I-EGFP of approximately 13 s...
  76. pmc Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to coordinate free movement with spatial information
    Gerald R V Hammond
    Department of Pharmacology, University of Cambridge, Cambridge, England, UK
    J Cell Biol 184:297-308. 2009
    ..quantitatively how these conflicting functional needs are optimized, we used different fluorescence recovery after photobleaching techniques to investigate inositol lipid-effector protein kinetics in terms of the real-time ..
  77. pmc Ena/VASP proteins have an anti-capping independent function in filopodia formation
    Derek A Applewhite
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Mol Biol Cell 18:2579-91. 2007
    ..Furthermore, the EVH1 domain, together with the GAB motif in the EVH2 domain, helps to maintain Ena/VASP at the growing barbed ends...
  78. ncbi Improving parameter estimation for cell surface FRAP data
    Omer Dushek
    Department of Mathematics and Institute of Applied Mathematics, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z2
    J Biochem Biophys Methods 70:1224-31. 2008
    b>Fluorescence Recovery After Photobleaching (FRAP) using the confocal laser scanning microscope has become a standard method used to determine the diffusion coefficient and mobile fraction of cell surface proteins...
  79. pmc Simulations of (an)isotropic diffusion on curved biological surfaces
    Ivo F Sbalzarini
    Institute of Computational Science, and Institute of Biochemistry, ETH Zurich, Switzerland
    Biophys J 90:878-85. 2006
    ..simulations are conducted on geometries reconstructed from real ER samples and are compared to fluorescence recovery after photobleaching experiments in the same ER samples using the transmembrane protein tsO45-VSV-G, C-terminally ..
  80. pmc Membrane lateral diffusion and capture of CFTR within transient confinement zones
    Ian R Bates
    Department of Physiology, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Biophys J 91:1046-58. 2006
    ..b>Fluorescence recovery after photobleaching and image correlation spectroscopy of the dye-labeled channels revealed a significant immobile ..
  81. doi Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network
    Elina Vladimirou
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    FEBS Lett 583:3690-6. 2009
    ..Integral membrane proteins within both the stromal and granal membranes are therefore highly constrained, possibly forming 'microdomains' that are sharply separated...
  82. pmc Measuring rotational diffusion of MHC class I on live cells by polarized FPR
    David R Fooksman
    Department of Chemistry, Colorado State University, Ft Collins, CO 80523, USA
    Biophys Chem 130:10-6. 2007
    ..This result points the way to use of engineered fluorescent fusion proteins to measure rotational diffusion in native cell membranes...
  83. pmc Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells
    Chris Molenaar
    Dept of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, Netherlands
    J Cell Biol 165:191-202. 2004
    ..Rather than the poly(A)+ RNA within speckles serving a stable structural role, our findings support the suggestion of a more active role of these regions in nuclear RNA metabolism and/or transport...
  84. ncbi Unrestricted diffusion of exogenous and endogenous PIP(2 )in baby hamster kidney and Chinese hamster ovary cell plasmalemma
    Alp Yaradanakul
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9040, USA
    J Membr Biol 220:53-67. 2007
    ..Diffusion coefficients determined by fluorescence recovery after photobleaching were similar for the two lipids and were higher in lawns, approximately 0...
  85. pmc Synaptic receptor trafficking: the lateral point of view
    F Jaskolski
    MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, Department of Anatomy, University Walk, Bristol, BS8 1TD, UK
    Neuroscience 158:19-24. 2009
    ..Here, we provide an overview of the principles and methodology of these techniques and highlight the contributions they have made to current understanding of protein mobility in the plasma membrane...
  86. pmc Analyses of in vivo interaction and mobility of two spliceosomal proteins using FRAP and BiFC
    Gul Shad Ali
    Department of Biology and Program in Molecular Plant Biology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 3:e1953. 2008
    ..of the U1-70K/SR protein complex using bimolecular fluorescence complementation (BiFC) and fluorescence recovery after photobleaching (FRAP)...
  87. ncbi A mathematical model to determine molecular kinetic rate constants under non-steady state conditions using fluorescence recovery after photobleaching (FRAP)
    Tanmay P Lele
    Vascular Biology Program, Karp Family Research Laboratories, 11 127, Children s Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    Biophys Chem 120:32-5. 2006
    b>Fluorescence recovery after photobleaching (FRAP) analyses of binding and unbinding of molecules that interact with insoluble scaffolds, such as the cytoskeleton and nuclear matrix, in living cells commonly assume that this process is at ..
  88. ncbi Quantifying RhoA facilitated trafficking of the epithelial Na+ channel toward the plasma membrane with total internal reflection fluorescence-fluorescence recovery after photobleaching
    Oleh Pochynyuk
    Department of Physiology, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    J Biol Chem 282:14576-85. 2007
    ..with two complementary imaging methods, total internal reflection fluorescence microscopy and fluorescence recovery after photobleaching, to study the mechanistic basis of RhoA actions on ENaC...
  89. pmc Monitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, 18 Library Drive, Building 18, Room 101, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:6536-41. 2006
    ..Our findings provide insight into the normal organization and dynamics of an ER chaperone and characterize the capacity of the ER to maintain homeostasis during acute changes in chaperone activity and availability...
  90. ncbi In vitro FRAP identifies the minimal requirements for Mad2 kinetochore dynamics
    Martin Vink
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy
    Curr Biol 16:755-66. 2006
    ..b>Fluorescence recovery after photobleaching (FRAP) revealed that the interaction of cytosolic Mad2 with kinetochores is highly dynamic, ..
  91. pmc Glucocorticoid receptor mutants demonstrate increased motility inside the nucleus of living cells: time of fluorescence recovery after photobleaching (FRAP) is an integrated measure of receptor function
    Tomoshige Kino
    Pediatric Endocrinology Section, Reproductive Biology and Medicine Branch, National Institute of Child Health and Human Development National Institutes of Health, Bethesda, MD 20892 1109, USA
    Mol Med 10:80-8. 2004
    ..Using fluorescence recovery after photobleaching (FRAP) analysis, all GR pathologic mutant receptors examined, as well as 2 synthetic GR mutants ..
  92. pmc Line FRAP with the confocal laser scanning microscope for diffusion measurements in small regions of 3-D samples
    Kevin Braeckmans
    Laboratory General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium
    Biophys J 92:2172-83. 2007
    We present a truly quantitative fluorescence recovery after photobleaching (FRAP) model for use with the confocal laser scanning microscope based on the photobleaching of a long line segment...
  93. pmc Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors
    Vinciane Tillemans
    Laboratory of Plant Cell and Molecular Biology, Department of Life Sciences, Institute of Botany, University of Liege, B 4000 Liege, Belgium
    Plant Cell 18:3218-34. 2006
    ..Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments...
  94. pmc A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleaching
    José Braga
    Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal
    Biophys J 92:2694-703. 2007
    b>Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study molecular dynamics inside living cells...
  95. ncbi FRAP beam-size analysis to measure palmitoylation-dependent membrane association dynamics and microdomain partitioning of Ras proteins
    Yoav I Henis
    Department of Neurobiochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Methods 40:183-90. 2006
    ..We describe the application of fluorescence recovery after photobleaching (FRAP) beam-size analysis to investigate the role of palmitoylation in the membrane targeting ..
  96. pmc Clustering of raft-associated proteins in the external membrane leaflet modulates internal leaflet H-ras diffusion and signaling
    Sharon Eisenberg
    Department of Neurobiochemistry, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Mol Cell Biol 26:7190-200. 2006
    ..Here, we employed fluorescence recovery after photobleaching to study the effects of antibody-mediated patching of influenza hemagglutinin (HA) proteins [..
  97. pmc Role of fascin in filopodial protrusion
    Danijela Vignjevic
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    J Cell Biol 174:863-75. 2006
    ..b>Fluorescence recovery after photobleaching of GFP-tagged wild-type and S39A fascin showed that dephosphorylated fascin underwent rapid ..
  98. ncbi Golgi structural stability and biogenesis depend on associated PKA activity
    Eloy Bejarano
    Department of Cell Biology, Faculty of Biology, University of Seville, Avd Reina Mercedes s n, 41012 Seville, Spain
    J Cell Sci 119:3764-75. 2006
    ..We conclude that protein kinase A activity plays a relevant role in the assembly and maintenance of a continuous Golgi ribbon from separated membrane stacks...
  99. pmc A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites
    Dorothee Molle
    IGMM CNRS UMR 5535, Montpellier, France
    Retrovirology 4:36. 2007
    ....
  100. ncbi Methods for measuring rates of protein binding to insoluble scaffolds in living cells: histone H1-chromatin interactions
    Tanmay Lele
    Department of Pathology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biochem 99:1334-42. 2006
    ..k(ON) and dissociation rate constant k(OFF) to be determined based on data obtained from fluorescence recovery after photobleaching (FRAP) analysis...
  101. pmc Src kinase activity and SH2 domain regulate the dynamics of Src association with lipid and protein targets
    Dmitry E Shvartsman
    Department of Neurobiochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Cell Biol 178:675-86. 2007
    ..Our observations indicate that the interactions of Src with lipid and protein targets are dynamic and that the kinase and SH2 domain cooperate in the membrane targeting of Src...

Research Grants89

  1. MECHANICAL LOADING AND BONE
    Hiroki Yokota; Fiscal Year: 2007
    ..strains using electronic speckle pattern interferometry as well as molecular transport using fluorescence recovery after photobleaching; (3) conducting bone histomorphometry to evaluate ex vivo data; and (4) examining load-driven ..
  2. Function and Expression of Connexins in the pre-Botzinger Complex
    JONATHAN KELTY; Fiscal Year: 2007
    ..carbenoxolone, or CBX) and a control agent (glycyrrhizic acid or GZA), will be evaluated by a fluorescence recovery after photobleaching (FRAP) microscopy technique...
  3. THE ROLES OF ESPINS IN HAIR CELL STEREOCILIA
    JAMES BARTLES; Fiscal Year: 2007
    ....
  4. MECHANICAL LOADING AND BONE
    Hiroki Yokota; Fiscal Year: 2010
    ..strains using electronic speckle pattern interferometry as well as molecular transport using fluorescence recovery after photobleaching;(3) conducting bone histomorphometry to evaluate ex vivo data;and (4) examining load-driven ..
  5. INTERMEDIATE FILAMENT--CELL SURFACE INTERACTIONS
    Robert Goldman; Fiscal Year: 1991
    ..or not a steady state or dynamic equilibrium exists in interphase cells through the use of fluorescence recovery after photobleaching (FRAP) experiments...
  6. Analysis of Z-band assembly and maintenance in living skeletal muscle cells
    JOSEPH WILLIAM contact SANGER; Fiscal Year: 2010
    ..FRAP (Fluorescence Recovery After Photobleaching) experiments will measure the changing dynamics of key proteins in the z-bodies and Z-bands ..
  7. Novel Epigenetic Marks in Mitosis
    KENNETH S contact ZARET; Fiscal Year: 2010
    ..Our recent mechanistic studies using fluorescence recovery after photobleaching (FRAP) show that FoxA and PARP binding to chromatin is far more stable than that of most other ..
  8. Control of Vascular Cell Motility by CaMKII
    Harold A Singer; Fiscal Year: 2010
    ..b>Fluorescence recovery after photobleaching (FRAP) analysis of GFP-tagged focal adhesion components will be used to assess CaMKII4-dependent ..
  9. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    DONALD BENEDICT DEFRANCO; Fiscal Year: 2010
    ..We have recently developed a novel in situ fluorescence recovery after photobleaching (FRAP) assay that led to the identification of molecular chaperones and their associated co-..
  10. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    DONALD BENEDICT DEFRANCO; Fiscal Year: 2010
    ..We have recently developed a novel in situ fluorescence recovery after photobleaching (FRAP) assay that led to the identification of molecular chaperones and their associated co-..
  11. MECHANICAL LOADING AND BONE
    Hiroki Yokota; Fiscal Year: 2009
    ..strains using electronic speckle pattern interferometry as well as molecular transport using fluorescence recovery after photobleaching; (3) conducting bone histomorphometry to evaluate ex vivo data; and (4) examining load-driven ..
  12. SIGNAL TRANSDUCTION AT FERTILIZATION
    Laurinda A Jaffe; Fiscal Year: 2010
    ..live follicle-enclosed oocytes with fluorescent tracers, and analysis by 2-photon microscopy and fluorescence recovery after photobleaching. These studies will be complemented by investigations of the phosphorylation of connexin 43 on ..
  13. Biophysical Basis of Organization and Dynamics of Prestin Membrane Complexes
    ROBERT RAPHAEL; Fiscal Year: 2009
    ..including fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging (FLIM), fluorescence recovery after photobleaching (FRAP) and single molecule imaging...
  14. Biophysical Basis of Organization and Dynamics of Prestin Membrane Complexes
    Robert M Raphael; Fiscal Year: 2010
    ..including fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging (FLIM), fluorescence recovery after photobleaching (FRAP) and single molecule imaging...
  15. Ligand Binding Kinetics and Cell Adhesion by Fc Receptor
    Cheng Zhu; Fiscal Year: 2005
    ..Measure the kinetics of Fc-gamma-R-IgG interactions by a new contact area FRAP (fluorescence recovery after photobleaching) method...
  16. Confocal Microscopy Imaging System
    Murad Ookhtens; Fiscal Year: 2007
    ..such as live cell and time-lapse imaging, imaging specimens labeled with 3 or 4 fluorophores, fluorescence recovery after photobleaching, or fluorescence resonance energy transfer, which are sorely needed by our user base...
  17. STRUCTURE AND FUNCTION OF PAXILLIN
    Christopher E Turner; Fiscal Year: 2010
    ..proteins in fibroblasts and utilize confocal fluorescence time-lapse microscopy, combined with Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Resonance Energy Transfer (FRET) analysis to evaluate cell morphology, ..
  18. STRUCTURE AND FUNCTION OF PAXILLIN
    Christopher Turner; Fiscal Year: 2009
    ..proteins in fibroblasts and utilize confocal fluorescence time-lapse microscopy, combined with Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Resonance Energy Transfer (FRET) analysis to evaluate cell morphology, ..
  19. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 1993
    ..Steady-state anisotropy imaging, b. Time- resolved microfluorimetry, c. Fluorescence recovery after photobleaching, and d. Total internal reflection microfluorimetry; and the development of two new methods: e...
  20. Reticulon Function in ER Morphology
    Gia Voeltz; Fiscal Year: 2011
    ..For these studies, we will be using fluorescence microscopy, immunoprecipitations, fluorescence recovery after photobleaching, and electron microscopy with immunogold labeling...
  21. Reticulon Function in ER Morphology
    Gia Voeltz; Fiscal Year: 2010
    ..For these studies, we will be using fluorescence microscopy, immunoprecipitations, fluorescence recovery after photobleaching, and electron microscopy with immunogold labeling...
  22. Solute Transport in the Bone Lacunar-Canalicular System
    Liyun Wang; Fiscal Year: 2010
    ..To this end, we recently developed a new imaging method based on Fluorescence Recovery After Photobleaching (FRAP) that allows measurement of solute movement in the bone LCS in situ and in real-time (Wang ..
  23. MOLECULAR MECHANISM OF PHOTORECEPTOR G PROTEIN SIGNALING
    Nikolai O Artemyev; Fiscal Year: 2010
    ..The mutant Gat1 models will be examined with EGFP imaging, immunofluorescence, and Fluorescence Recovery After Photobleaching (FRAP) analysis of lateral and longitudinal diffusion...
  24. Solute Transport in the Bone Lacunar-Canalicular System
    Liyun Wang; Fiscal Year: 2009
    ..To this end, we recently developed a new imaging method based on Fluorescence Recovery After Photobleaching (FRAP) that allows measurement of solute movement in the bone LCS in situ and in real-time (Wang ..
  25. Solute Transport in the Bone Lacunar-Canalicular System
    Liyun Wang; Fiscal Year: 2007
    ..To this end, we recently developed a new imaging method based on Fluorescence Recovery After Photobleaching (FRAP) that allows measurement of solute movement in the bone LCS in situ and in real-time (Wang ..
  26. Olympus FV1000 Confocal Fluorescence Microscope
    James Goldenring; Fiscal Year: 2007
    ..unique capabilities for live cell investigations including enhanced experimental approaches to Fluorescence Recovery after Photobleaching (FRAP), imaging in the ultraviolet range, and the ability to utilize photoactivatable probes for ..
  27. Advanced Immunocytochemistry, In Situ Hybridization and Live Cell Imaging
    TERRI GRODZICKER; Fiscal Year: 2007
    ..to localize, quantify, and analyze proteins in living cells using standard fluorescence, fluorescence recovery after photobleaching, and resonance energy transfer...
  28. MYOFIBRILLOGENESIS IN LIVING CARDIAC MUSCLE CELLS
    Joseph Sanger; Fiscal Year: 2006
    ..Images of live cells will be analyzed by optical techniques, such as Fluorescence Recovery after Photobleaching (FRAP), confocal and deconvolution microscopy...
  29. MITOSIS IN NORMAL AND NEOPLASTIC CELLS
    BILL BRINKLEY; Fiscal Year: 2006
    ..Additional experiments and novel methodology will be introduced such as Fluorescence Recovery After Photobleaching (FRAP) to track the movement and dynamics of bioluminescent proteins in live cells and new FIAsH-..
  30. TRANSDUCIN INTERACTIONS WITH PHOTORECEPTOR MEMBRANES
    Theodore Wensel; Fiscal Year: 2007
    ..Specific Aim 2 is to use Fluorescence Recovery After Photobleaching (FRAP) and Fluctuation Correlation Spectroscopy (FCS) in transgenic frogs to study the diffusion ..
  31. TRANSDUCIN INTERACTIONS WITH PHOTORECEPTOR MEMBRANES
    Theodore G Wensel; Fiscal Year: 2010
    ..Specific Aim 2 is to use Fluorescence Recovery After Photobleaching (FRAP) and Fluctuation Correlation Spectroscopy (FCS) in transgenic frogs to study the diffusion ..
  32. ZEISS LSM 510 CONFOCAL MICROSCOPE SYSTEM
    Richard Kurten; Fiscal Year: 2005
    ..FRET, multiple color live cell tracking experiments, organelle-specific photobleaching (FRAP, fluorescence recovery after photobleaching and FLIP, fluorescence loss in photobleaching) as well as excellent standard confocal microscopy ..
  33. Asymmetric division as a morphological checkpoint for B. subtilis sporulation
    TINYA FLEMING; Fiscal Year: 2007
    ..Protein-protein interactions at the septum may also regulate aF and aE activity. The use of Fluorescence Recovery After Photobleaching (FRAP) will identify changes in protein mobility, which varies as a result of changes in protein ..