polyacrylamide gel electrophoresis

Summary

Summary: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

Top Publications

  1. ncbi Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry
    Rainy Mears
    Cancer Research UK Clinical Centre, St James s University Hospital, Leeds, UK
    Proteomics 4:4019-31. 2004
  2. ncbi Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expression
    A T Behan
    Department of Psychiatry, Royal College of Surgeons in Ireland, RCSI ERC, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland
    Mol Psychiatry 14:601-13. 2009
  3. ncbi A generic protein purification method for protein complex characterization and proteome exploration
    G Rigaut
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Biotechnol 17:1030-2. 1999
  4. pmc Oligomerization of the integrin alphaIIbbeta3: roles of the transmembrane and cytoplasmic domains
    R Li
    Department of Biochemistry and Biophysics and Hematology Oncology Division, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 98:12462-7. 2001
  5. ncbi Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition
    M T Bayliss
    Royal Veterinary College, Royal College Street, London NW1 OTU, United Kingdom
    J Biol Chem 274:15892-900. 1999
  6. ncbi Snapshots of DsbA in action: detection of proteins in the process of oxidative folding
    Hiroshi Kadokura
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 303:534-7. 2004
  7. pmc Biochemical and functional similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease
    G J Gleich
    Proc Natl Acad Sci U S A 83:3146-50. 1986
  8. pmc The many applications of acid urea polyacrylamide gel electrophoresis to studies of tRNAs and aminoacyl-tRNA synthetases
    Caroline Köhrer
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 68 671, Cambridge, MA 02139, USA
    Methods 44:129-38. 2008
  9. ncbi Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin
    Graciela Lorca
    Centro de Referencia para Lactobacilos CERELA, CONICET, Tucmán, Argentina
    FEMS Microbiol Lett 206:31-7. 2002
  10. ncbi Identification, proteomic profiling, and origin of ram epididymal fluid exosome-like vesicles
    Jean Luc Gatti
    Equipe Gamètes Males et Fertilité, URM 6175 INRA CNRS Université de TOURS Haras Nationaux, Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique de Nouzilly, Monnaie 37380, France
    Biol Reprod 72:1452-65. 2005

Detail Information

Publications333 found, 100 shown here

  1. ncbi Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry
    Rainy Mears
    Cancer Research UK Clinical Centre, St James s University Hospital, Leeds, UK
    Proteomics 4:4019-31. 2004
    ..The two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) protein profiles of exosomes from the two cell lines were highly comparable and ..
  2. ncbi Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expression
    A T Behan
    Department of Psychiatry, Royal College of Surgeons in Ireland, RCSI ERC, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland
    Mol Psychiatry 14:601-13. 2009
    ..methods, two-dimensional difference gel electrophoresis and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by reverse phase-liquid chromatography-tandem mass spectrometry (RP-LC-MS/MS) (gel ..
  3. ncbi A generic protein purification method for protein complex characterization and proteome exploration
    G Rigaut
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Biotechnol 17:1030-2. 1999
    ..Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms...
  4. pmc Oligomerization of the integrin alphaIIbbeta3: roles of the transmembrane and cytoplasmic domains
    R Li
    Department of Biochemistry and Biophysics and Hematology Oncology Division, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 98:12462-7. 2001
    ..Thus, these regions of the protein structure may contribute to the clustering of integrins that accompanies cellular adhesion...
  5. ncbi Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition
    M T Bayliss
    Royal Veterinary College, Royal College Street, London NW1 OTU, United Kingdom
    J Biol Chem 274:15892-900. 1999
    ..Furthermore, these changes in composition are a consequence of both extracellular, post-translational processing of the core protein of aggrecan and changes in the sulfotransferase activity of the chondrocyte...
  6. ncbi Snapshots of DsbA in action: detection of proteins in the process of oxidative folding
    Hiroshi Kadokura
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 303:534-7. 2004
    ..Alteration of the cis proline, highly conserved among thioredoxin superfamily members, may be useful for the detection of substrates and intermediate complexes in other systems...
  7. pmc Biochemical and functional similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease
    G J Gleich
    Proc Natl Acad Sci U S A 83:3146-50. 1986
    ..15 micrograms when injected into the cisterna magna; the proteins were comparable in their activities. These results indicate that EDN and ECP are related proteins and suggest that they derived from genes associated with the RNase family...
  8. pmc The many applications of acid urea polyacrylamide gel electrophoresis to studies of tRNAs and aminoacyl-tRNA synthetases
    Caroline Köhrer
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 68 671, Cambridge, MA 02139, USA
    Methods 44:129-38. 2008
    Here we describe the many applications of acid urea polyacrylamide gel electrophoresis (acid urea PAGE) followed by Northern blot analysis to studies of tRNAs and aminoacyl-tRNA synthetases...
  9. ncbi Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin
    Graciela Lorca
    Centro de Referencia para Lactobacilos CERELA, CONICET, Tucmán, Argentina
    FEMS Microbiol Lett 206:31-7. 2002
    ..Monoclonal antibodies showed that Fn binds to a 15-kDa protein, while Cn-I binds to proteins of 45 and 58 kDa...
  10. ncbi Identification, proteomic profiling, and origin of ram epididymal fluid exosome-like vesicles
    Jean Luc Gatti
    Equipe Gamètes Males et Fertilité, URM 6175 INRA CNRS Université de TOURS Haras Nationaux, Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique de Nouzilly, Monnaie 37380, France
    Biol Reprod 72:1452-65. 2005
    ..The main membrane-linked proteins of the vesicles were not retrieved in the extract from cauda or ejaculated sperm, suggesting that these vesicles did not fuse with sperm in vivo...
  11. pmc Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography cou
    Richard C Jones
    Divisions of Microbiology, Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079 9502, USA
    J Bacteriol 190:5265-78. 2008
    One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro ..
  12. ncbi The nuclear Hat1p/Hat2p complex: a molecular link between type B histone acetyltransferases and chromatin assembly
    Xi Ai
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    Mol Cell 14:195-205. 2004
    ..The association of Hif1p with acetylated H4 requires Hat1p and Hat2p providing a link between type B histone acetyltransferases and chromatin assembly...
  13. ncbi Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions
    Daisuke Miyoshi
    Frontier Institute for Biomolecular Engineering Research, Konan University, 8 9 1 Okamoto, Higashinada ku, Kobe 658 8501, Japan
    J Am Chem Soc 131:3522-31. 2009
    ..2 +/- 0.2 kcal mol(-1), respectively. Furthermore, it was revealed that the opposing effects of molecular crowding on the Hoogsteen and Watson-Crick base pairs were due to different behaviors of water molecules binding to the DNA strands...
  14. ncbi A novel WD repeat protein component of the methylosome binds Sm proteins
    Westley J Friesen
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    J Biol Chem 277:8243-7. 2002
    ....
  15. pmc Mapping of the p53 and mdm-2 interaction domains
    J Chen
    Department of Molecular Biology, Princeton University, New Jersey 08544 1014
    Mol Cell Biol 13:4107-14. 1993
    ..This region contains the transactivation domain of p53, suggesting that mdm-2 may inhibit p53 function by disrupting its interaction with the general transcription machinery...
  16. pmc GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies
    R P McEver
    St Francis Medical Research Institute, University of Oklahoma Health Science Center, Oklahoma City 73104
    J Clin Invest 84:92-9. 1989
    ..The restricted expression of GMP-140 in secretory granules of platelets and endothelium suggests that it has a specific function in the vascular system rather than a general role related to inducible secretion...
  17. ncbi A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
    C R R Ramos
    Centro de Biotecnologia, Instituto Butantan, Sao Paulo, SP, Brazil
    Braz J Med Biol Res 37:1103-9. 2004
    ..Thus, although proteins expressed by pRSET plasmids also have a hexa-histidine tag, the fusion peptide is much longer and may represent a problem for some recombinant proteins...
  18. ncbi Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.)
    Leo van Overbeek
    Plant Research International, Wageningen, The Netherlands
    FEMS Microbiol Ecol 64:283-96. 2008
    ..Its prevalence was affected by plant growth stage and experiment rather than by plant genotype. It was concluded that plant growth stage overwhelmed any effect of plant genotype on the bacterial communities associated with potato...
  19. pmc The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition
    W G Whitfield
    Department of Biochemistry, The University, Dundee, Scotland
    EMBO J 9:2563-72. 1990
    ..These observations suggest that cyclin A has a role in the G2-M transition that is independent of spindle formation, and that entry into anaphase is a key requirement for the degradation of cyclin B...
  20. pmc Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
    C Lange
    , Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt, Germany
    EMBO J 20:6591-600. 2001
    ..Site-directed mutagenesis of ligating residues confirmed the importance of the phosphatidylinositol- and cardiolipin-binding sites...
  21. pmc Apoptosis induced by a human milk protein
    A Hakansson
    Department of Medical Microbiology, Lund University, Sweden
    Proc Natl Acad Sci U S A 92:8064-8. 1995
    ..Finally, analysis of the mechanism by which multimeric alpha-lactalbumin induces apoptosis in transformed epithelial cells could lead to the design of antitumor agents...
  22. ncbi Use of polymerase chain reaction techniques and sodium dodecyl sulfate-polyacrylamide gel electrophoresis for differentiation of oral Lactobacillus species
    R Teanpaisan
    Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Thailand
    Oral Microbiol Immunol 21:79-83. 2006
    ..polymorphism analysis of polymerase chain reaction (PCR)-amplified 16S rRNA (16S rRNA gene PCR-RFLP) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for the identification of 13 reference strains of Lactobacillus.
  23. pmc Subinhibitory concentrations of linezolid reduce Staphylococcus aureus virulence factor expression
    Katussevani Bernardo
    Institute for Medical Microbiology, Immunology, and Hygiene, Medical Center, University of Cologne, Cologne, Germany
    Antimicrob Agents Chemother 48:546-55. 2004
    ..on the secretion of exotoxins by Staphylococcus aureus was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry and ..
  24. ncbi Suppression of sodium dodecyl sulfate-polyacrylamide gel electrophoresis sample preparation artifacts for analysis of IgG4 half-antibody
    Frederick R Taylor
    Biogen Idec, Inc, 14 Cambridge Center, Cambridge, MA 02142, USA
    Anal Biochem 353:204-8. 2006
    ..portion (5-50%) of a heavy chain-light chain dimer ("half-antibody") on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), in which the heavy chain is not covalently linked through the hinge disulfides to ..
  25. ncbi Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein
    H Hirano
    National Institute of Agrobiological Resources, Tsukuba, Japan
    Electrophoresis 11:573-80. 1990
    ..Using this technique, we determined the amino acid sequences of the basic 7S globulin (Bg), an insoluble protein present in soybean seeds. Based on sequence determination, the cDNA-encoding Bg could be easily cloned and characterized...
  26. ncbi A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cells
    Patrick H K Ngai
    Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Biochem Biophys Res Commun 314:988-93. 2004
    ..125 to 50mM) including NaCl, KCl, CaCl(2), MgCl(2), ZnCl(2), MnCl(2), and AlCl(3). However, it was potentiated by 12.5-50mM FeCl(3). The lectin was devoid of HIV-1 reverse transcriptase inhibitory and antifungal activities...
  27. ncbi Analysis of the native quaternary structure of vanilloid receptor 1
    N Kedei
    Laboratory of Cellular Carcinogenesis and Tumor Promotion, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:28613-9. 2001
    ..in dorsal root ganglion cells, we analyzed oligomer formation using perfluoro-octanoic acid polyacrylamide gel electrophoresis. Results were confirmed both with chemical cross-linking agents as well as through endogenous cross-..
  28. ncbi A fluorescent carbazole derivative: high sensitivity for quadruplex DNA
    Cheng Chung Chang
    Institute of Atomic and Molecular Sciences, Academia Sinica, PO Box 23 166, Taipei, 10764, Taiwan, Republic of China
    Anal Chem 75:6177-83. 2003
    ..Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T(2)AG(3))(4) can be revealed by BMVC...
  29. ncbi Gluing the respiratory chain together. Cardiolipin is required for supercomplex formation in the inner mitochondrial membrane
    Mei Zhang
    Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225, USA
    J Biol Chem 277:43553-6. 2002
    ..Complexes III and IV associate to form a supercomplex that can be displayed using blue native polyacrylamide gel electrophoresis. Both homodimeric complexes contain tightly associated cardiolipin (CL) required for function...
  30. pmc Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants
    Welington L Araujo
    Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, Brazil
    Appl Environ Microbiol 68:4906-14. 2002
    ..A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC...
  31. ncbi Analysis of the subunit composition of complex I from bovine heart mitochondria
    Joe Carroll
    Medical Research Council Dunn Human Nutrition Unit, The Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Mol Cell Proteomics 2:117-26. 2003
    ..It is unlikely that any more subunits of the bovine complex remain undiscovered. Therefore, the intact enzyme is a complex of 46 subunits, and, assuming there is one copy of each subunit in the complex, its mass is 980 kDa...
  32. ncbi Catalogue of soluble proteins in human vitreous humor by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization mass spectrometry including seven angiogenesis-regulating factors
    Reiko Koyama
    Department of Ophthalmology, Osaka Medical College, 2 7 Daigakucho, Takatsuki, Osaka 569 8686, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 792:5-21. 2003
    ..To improve the recovery of proteins in vitreous, we applied one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-PAGE)...
  33. pmc A systematic characterization of mitochondrial proteome from human T leukemia cells
    Karim Rezaul
    Department of Cell Biology, Center for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Mol Cell Proteomics 4:169-81. 2005
    ..Thus, we conclude that this approach is suitable for characterizing subcellular proteomes form multiple cells and tissues...
  34. pmc Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota
    Inés Martínez
    Department of Food Science and Technology, University of Nebraska, Lincoln, 68583 0919, USA
    Appl Environ Microbiol 75:4175-84. 2009
    ..This study provides evidence that modulation of the gut microbiota-host metabolic interrelationship by dietary intervention has the potential to improve mammalian cholesterol homeostasis, which has relevance for cardiovascular health...
  35. ncbi Preparation of coated nanoparticles for a new mucosal vaccine delivery system
    Olga Borges
    Leiden Amsterdam Center for Drug Research, Division of Pharmaceutical Technology, P O Box 9502, 2300 RA Leiden, The Netherlands
    Int J Pharm 299:155-66. 2005
    ..The optimisation of the coating process resulted in 35% (w/w) for the loading capacity of the coated particles. SEM investigations confirmed a suitable size of the coated nanoparticles for the uptake by M-cells...
  36. ncbi Deamidation affects structural and functional properties of human alphaA-crystallin and its oligomerization with alphaB-crystallin
    Ratna Gupta
    Department of Physiological Optics, University of Alabama at Birmingham, Birmingham, Alabama 35294 4390, USA
    J Biol Chem 279:44258-69. 2004
    ....
  37. ncbi The nonspecific DNA-binding and -bending proteins HMG1 and HMG2 promote the assembly of complex nucleoprotein structures
    T T Paull
    Molecular Biology Institute, University of California Los Angeles 90024
    Genes Dev 7:1521-34. 1993
    ..A general role for HMG1 and HMG2 in chromatin structure is also suggested by their ability to wrap DNA duplexes into highly compact forms...
  38. ncbi Structural polymorphism of telomeric DNA regulated by pH and divalent cation
    Daisuke Miyoshi
    Department of Chemistry, Faculty of Science and Engineering, Konan University, Higashinada ku, Kobe, Japan
    Nucleosides Nucleotides Nucleic Acids 22:203-21. 2003
    ....
  39. ncbi Roles of a membrane-bound caleosin and putative peroxygenase in biotic and abiotic stress responses in Arabidopsis
    Mark Partridge
    Biotechnology Unit, Division of Biological Sciences, University of Glamorgan, Treforest, CF37 1DL, United Kingdom
    Plant Physiol Biochem 47:796-806. 2009
    ..We propose that Clo-3 is part of an oxylipin pathway induced by multiple stresses and may also generate fatty acid derived anti-fungal compounds for plant defence...
  40. ncbi Inhibition of HIV-1 IIIB and clinical isolates by human parotid, submandibular, sublingual and palatine saliva
    Jan G M Bolscher
    Department of Dental Basic Sciences, Academic Center for Dentistry Amsterdam, The Netherlands
    Eur J Oral Sci 110:149-56. 2002
    ..In conclusion, saliva inhibits HIV-1-infection by the action of high molecular weight components in combination with low molecular weight components from serous as well as mucous saliva, affecting different stages of the infection cycle...
  41. ncbi Partial characterization of bacteriocins produced by human Lactococcus lactis and Pediococccus acidilactici isolates
    M Millette
    INRS Institut Armand Frappier, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre, 531, boulevard des Prairies, Laval, Quebec, Canada, H7V 1B7
    J Appl Microbiol 102:274-82. 2007
    ..The aim of this study was to isolate bacteriocin-producing lactic acid bacteria (LAB) from human intestine...
  42. ncbi Electrophoresis techniques to investigate defects in oxidative phosphorylation
    Maria Antonietta Calvaruso
    Nijmegen Center for Mitochondrial Disorders at the Department of Pediatrics, Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Methods 46:281-7. 2008
    ..Here we provide a practical guide on how to set-up the basic technique to study OXPHOS defects in patient-derived cells and tissues...
  43. ncbi Presence of tear lipocalin and other major proteins in lacrimal fluid of rabbits
    Ana Maria Azzarolo
    Charles E Schmidt Biomedical Science Department, Florida Atlantic University, Building 71, Room 145, 777 Glades Road, P O Box 3091, Boca Raton, FL 33431 0991, USA
    Comp Biochem Physiol B Biochem Mol Biol 138:111-7. 2004
    ....
  44. ncbi Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly
    Claudia Carissimi
    Dulbecco Telethon Institute, Institute of Cell Biology, Consiglio Nazionale delle Ricerche, 00016 Monterotondo Scalo, Rome, Italy
    J Biol Chem 281:8126-34. 2006
    ..These results demonstrate that Gemin8 is a novel integral component of the SMN complex and extend the repertoire of cellular proteins involved in the pathway of snRNP biogenesis...
  45. ncbi A unique role of an amino terminal 16-residue region of long-type GATA-6
    Mika Takeda
    Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565 0871
    J Biochem 135:639-50. 2004
    ..These results suggest that the translation of this region (Glu-31-Cys-46) could be critical for the apparent production of long-type GATA-6. We also demonstrated that long-type GATA-6 is potentially more active than the short-type...
  46. ncbi Analysis of avocado allergen (Prs a 1) IgE-binding peptides generated by simulated gastric fluid digestion
    Araceli Diaz-Perales
    Departamento de Biotecnologia, E T S Ingenieros Agronomos, UPM, Madrid, Spain
    J Allergy Clin Immunol 112:1002-7. 2003
    ..Allergenic plant class I chitinases, such as avocado Prs a 1, are the panallergens involved in the latex-fruit syndrome. Previous reports indicated their susceptibility to simulated gastric fluid (SGF) digestion...
  47. ncbi Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis
    E Mortz
    MDS Proteomics, Odense, Denmark
    Proteomics 1:1359-63. 2001
    ..Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry...
  48. ncbi Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon alpha2b
    Suzanne Hermeling
    Central Laboratory Animal Institute, Utrecht University, Utrecht, The Netherlands
    Pharm Res 22:1997-2006. 2005
    ..This study was conducted to study the influence of protein structure on the immunogenicity in wild-type and immune tolerant mice of well-characterized degradation products of recombinant human interferon alpha2b (rhIFNalpha2b)...
  49. ncbi Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregation
    Suzanne Hermeling
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Pharm Sci 95:1084-96. 2006
    ..gel permeation chromatography (GPC), dynamic light scattering (DLS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, and ELISA...
  50. ncbi Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity
    F Gharahdaghi
    The Rockefeller University Protein DNA Technology Center, New York, NY 10021, USA
    Electrophoresis 20:601-5. 1999
    ....
  51. ncbi Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of catalytic domains compared with presenilins
    Elena Friedmann
    Institute of Biochemistry, Swiss Federal Institute of Technology ETH, ETH Hoenggerberg, 8093 Zurich, Switzerland
    J Biol Chem 279:50790-8. 2004
    ..Other than presenilins, SPPL2a, -2b, -2c, and -3 are therefore predicted to cleave type II-oriented substrate peptides like the prototypic protease SPP...
  52. ncbi Disparate LDL phenotypic classification among 4 different methods assessing LDL particle characteristics
    Wayne Ensign
    Naval Health Research Center, San Diego, CA, USA
    Clin Chem 52:1722-7. 2006
    ....
  53. ncbi A second IgG-binding protein in Staphylococcus aureus
    L Zhang
    Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Microbiology 144:985-91. 1998
    ..Furthermore, it is shown that Sbi is highly expressed in strain Newman 4, which shows that IgG-binding activity in S. aureus can be mediated by proteins other than protein A...
  54. ncbi On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis
    Thomas Raschle
    ETH Zurich, Institut für Pflanzenwissenschaften, Zurich, Switzerland
    J Biol Chem 280:32291-300. 2005
    ..The results are discussed in light of the nature of the interaction of the two components of the enzyme complex...
  55. ncbi Degradation of Mcl-1 by granzyme B: implications for Bim-mediated mitochondrial apoptotic events
    Jie Han
    Departments of Pathology and Plastic Surgery, The University of Pittsburgh School of Medicine and The University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 279:22020-9. 2004
    ..The findings presented herein imply that Mcl-1L degradation by either GrB or caspase-3 interferes with Bim sequestration by Mcl-1L...
  56. pmc Structure and ion channel activity of the human respiratory syncytial virus (hRSV) small hydrophobic protein transmembrane domain
    Siok Wan Gan
    School of Biological Sciences, Nanyang Technological University, 637551 Singapore
    Protein Sci 17:813-20. 2008
    ..We provide a model for this pore, which should be useful in mutagenesis studies to elucidate its role during the virus cycle...
  57. ncbi A bacterially expressed particulate hepatitis E vaccine: antigenicity, immunogenicity and protectivity on primates
    Shao W Li
    The Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
    Vaccine 23:2893-901. 2005
    ..The efficacy of the vaccine was essentially the same for the homologous genotype 1 virus and heterologous genotype 4 virus...
  58. ncbi Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei
    Narisara Anuntagool
    Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand
    Microbiol Immunol 46:143-50. 2002
    ..e., the presence or absence of exopolysaccharide and the types of lipopolysaccharide. The heterogeneity of the LPS from these two closely related organisms is most likely related to the differences in its O-polysaccharide side chain...
  59. ncbi Design and self-assembly of two-dimensional DNA crystals
    E Winfree
    Computation and Neural Systems, California Institute of Technology, Pasadena 91125, USA
    Nature 394:539-44. 1998
    ..The patterned crystals have been visualized by atomic force microscopy...
  60. ncbi A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa
    G Q Zhang
    State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China
    Acta Biochim Pol 56:415-21. 2009
    ..In the case of the lectin from P. aurivella, its antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities have not been determined...
  61. ncbi Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism
    Nicolas Rouhier
    Unité Mixte de Recherche IaM INRA UHP Nancy I, Biochimie et Biologie Moléculaire Végétales, Universite Henri Poincare, 54506 Vandoeuvre Cedex, France
    J Biol Chem 277:13609-14. 2002
    ..Based on these experimental results, a catalytic mechanism is proposed to explain the Grx- and Trx-dependent activities of poplar Prx...
  62. doi Two-dimensional blue native/blue native polyacrylamide gel electrophoresis for the characterization of mitochondrial protein complexes and supercomplexes
    Stephanie Sunderhaus
    Abt Angewandte Genetik, Naturwissenschafiliche Fakultät, Universitat Hannover, Germany
    Methods Mol Biol 372:315-24. 2007
    Blue native polyacrylamide gel electrophoresis (BN-PAGE) employs the dye Coomassie for the labeling of proteins and protein complexes under native conditions...
  63. pmc A novel strategy involved in [corrected] anti-oxidative defense: the conversion of NADH into NADPH by a metabolic network
    Ranji Singh
    Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ontario, Canada
    PLoS ONE 3:e2682. 2008
    ....
  64. pmc Delivery of the non-membrane-permeative antibiotic gentamicin into mammalian cells by using Shigella flexneri membrane vesicles
    J L Kadurugamuwa
    Canadian Bacterial Diseases Network, Department of Microbiology, College of Biological Science, University of Guelph, Ontario, Canada
    Antimicrob Agents Chemother 42:1476-83. 1998
    ..Being bilayered, with outer faces composed of LPS and Ipa proteins, these MVs were readily engulfed by the otherwise impermeable membranes and eventually liberated their contents into the cytoplasmic substance of the host tissue...
  65. ncbi A sodium dodecyl sulfate-polyacrylamide gel electrophoresis-liquid chromatography tandem mass spectrometry analysis of bovine cartilage tissue response to mechanical compression injury and the inflammatory cytokines tumor necrosis factor alpha and interle
    Anna L Stevens
    Massachusetts Institute of Technology, Cambridge 02139, USA
    Arthritis Rheum 58:489-500. 2008
    ....
  66. ncbi Microfluidic polyacrylamide gel electrophoresis with in situ immunoblotting for native protein analysis
    Mei He
    Department of Bioengineering, University of California, Berkeley, California 94720, USA
    Anal Chem 81:8177-84. 2009
    ..Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting...
  67. ncbi "LaneSpector", a tool for membrane proteome profiling based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis/liquid chromatography-tandem mass spectrometry analysis: application to Listeria monocytogenes membrane proteins
    Dirk Wehmhöner
    Department of Cell Biology, GBF German Research Centre for Biotechnology, Braunschweig, Germany
    Electrophoresis 26:2450-60. 2005
    ..While the workflow based on two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) can be applied for many subproteomes and comprises well-established strategies for data ..
  68. ncbi A proteomic analysis of human bile
    Troels Zakarias Kristiansen
    McKusick Nathans Institute of Genetic Medicine and Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Cell Proteomics 3:715-28. 2004
    ..In combination with "tagging" approaches for differential proteomics, our method could be used for identification of cancer biomarkers from any body fluid...
  69. ncbi Characterization of the in vivo forms of lacrimal-specific proline-rich proteins in human tear fluid
    Kim Y C Fung
    Biochemical Mass Spectrometry Facility University of Colorado Health Sciences Center Denver, CO, USA
    Proteomics 4:3953-9. 2004
    ..None of these had previously been identified in tear. Our study highlights the dangers inherent in proteomic strategies that assign an identity to a protein based on limited coverage of tryptic peptides...
  70. pmc Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes
    Peter De Wulf
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 17:2902-21. 2003
    ..In addition, we propose that the three complexes have different functions with respect to force generation and MT attachment...
  71. ncbi A new algorithm for the simulation of sodium dodecil sulfate two-dimensional polyacrylamide gel electrophoresis data sets
    Emilio Marengo
    Department of Environmental and Life Sciences, University of Eastern Piedmont, viale Teresa Michel 11, Alessandria, Italy
    J Proteome Res 9:1864-72. 2010
    A new algorithm for generating simulated sodium dodecil sulfate two-dimensional polyacrylamide gel electrophoresis (SDS 2D-PAGE) map images was developed...
  72. ncbi Energetics and cooperativity of tertiary hydrogen bonds in RNA structure
    S K Silverman
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado at Boulder 80309 0215, USA
    Biochemistry 38:8691-702. 1999
    ..4 to -0.5 kcal/mol per tertiary hydrogen bond may be more generally applicable to RNA folding...
  73. ncbi Cystatins in human tear fluid
    T Barka
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, NY 10029
    Curr Eye Res 10:25-34. 1991
    ..Less abundant additional cystatin-like immuno-reactive proteins were detected by using the two latter antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)..
  74. ncbi Critical residues for structure and catalysis in short-chain dehydrogenases/reductases
    Charlotta Filling
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 277:25677-84. 2002
    ..This extends the previously recognized catalytic triad of Ser-Tyr-Lys residues to form a tetrad of Asn-Ser-Tyr-Lys in the majority of characterized short-chain dehydrogenases/reductase enzymes...
  75. pmc Inositol pyrophosphates and their unique metabolic complexity: analysis by gel electrophoresis
    Oriana Losito
    Department of Cell and Developmental Biology, Medical Research Council Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London, London, United States of America
    PLoS ONE 4:e5580. 2009
    ..Though likely involved in a wide range of cellular functions, the study of inositol pyrophosphates has suffered from a lack of readily available methods for their analysis...
  76. ncbi Reliable identification of clinically prevalent species and subspecies of staphylococci by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis
    Olinda Cabral da Silva Santos
    Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    Diagn Microbiol Infect Dis 64:1-5. 2009
    We identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 10 species and 5 subspecies of Staphylococcus among 139 clinical isolates and compared it with conventional tests...
  77. pmc Resolving mitochondrial protein complexes using nongradient blue native polyacrylamide gel electrophoresis
    Liang Jun Yan
    Department of Pharmacology and Neuroscience and Institute for Aging and Alzheimer s Disease Research, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX 76107, USA
    Anal Biochem 389:143-9. 2009
    Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful technique for separation and proteomics analysis of high-molecular-weight protein complexes...
  78. ncbi Polyacrylamide gel electrophoresis of fluorophore-labeled hyaluronan and chondroitin sulfate disaccharides: application to the analysis in cells and tissues
    Evgenia G Karousou
    Department of Experimental and Clinical Biomedical Sciences, University of Insubria, Varese, Italy
    Electrophoresis 25:2919-25. 2004
    This report describes a new formulation of polyacrylamide gel electrophoresis of fluorophore-labeled saccharides (PAGEFS) for the analysis of hyaluronan (HA) and chondroitin sulfate (CS) Delta-disaccharides...
  79. ncbi Investigating the fate of activated sludge extracellular proteins in sludge digestion using sodium dodecyl sulfate polyacrylamide gel electrophoresis
    Chul Park
    Department of Civil and Environmental Engineering, University of Massachusetts, Amherst, MA 01003, USA
    Water Environ Res 80:2219-27. 2008
    ..investigated using three different cation-associated extraction methods and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
  80. ncbi Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes
    Mahel Zeghouf
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6
    J Proteome Res 3:463-8. 2004
    ..We also present an adaptation of the SPA system for the efficient purification and identification of protein complexes in E. coli and, potentially, other bacteria...
  81. ncbi In situ alkylation with acrylamide for identification of cysteinyl residues in proteins during one- and two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis
    Reiko Mineki
    Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan
    Proteomics 2:1672-81. 2002
    Cysteinyl residues in proteins were alkylated with acrylamide during sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) to yield a thioether derivative, cys-S-beta-propionamide (PAM cys)...
  82. ncbi Purification of a novel low-molecular-mass laccase with HIV-1 reverse transcriptase inhibitory activity from the mushroom Tricholoma giganteum
    H X Wang
    Department of Microbiology, China Agricultural University, and State Key Laboratory of Agrobiotechnology, Beijing, China
    Biochem Biophys Res Commun 315:450-4. 2004
    ..The laccase was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-cellulose. It inhibited HIV-1 reverse transcriptase with an IC(50) of 2.2 microM...
  83. ncbi Isolation of cucurmoschin, a novel antifungal peptide abundant in arginine, glutamate and glycine residues from black pumpkin seeds
    H X Wang
    Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China
    Peptides 24:969-72. 2003
    A novel antifungal peptide, with a molecular mass of 8 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in gel filtration on Superdex 75 and designated cucurmoschin, was isolated from the seeds of the black pumpkin...
  84. ncbi Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and forster resonance energy transfer suggest weak interactions between fibroblast growth factor receptor 3 (FGFR3) transmembrane domains in the absence of extracellular domains and ligands
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 44:352-60. 2005
    ..We have performed sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with peptides produced via solid-phase peptide synthesis that correspond to the TM domain ..
  85. ncbi Cardiolipin stabilizes respiratory chain supercomplexes
    Kathy Pfeiffer
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    J Biol Chem 278:52873-80. 2003
    ..We show that a cardiolipin-deficient strain harbored almost inactive resting cytochrome c oxidase in the membrane. Transition to the fully active pulsed state occurred on a minute time scale...
  86. ncbi Fragmentation pattern of mucins in normal and diseased gastric mucosae: a glycoprotein fractionates with gastric mucins purified from mucosal scrapings of cancer and peptic ulcer patients
    A S Mall
    Department of Surgery and MRC Liver Research Centre, University of Cape Town Medical School, Cape Town, South Africa
    Digestion 60:216-26. 1999
    ..The aim of this study was a biochemical characterisation of gastric mucins in this disease, compared with gastric ulceration and controls from transplant donors...
  87. ncbi Electrophoretic mobility of Alzheimer's amyloid-beta peptides in urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    John K Kawooya
    Lead Discovery Technologies Protein Production, JR1 2201, Aventis Pharmaceuticals, 1041 Route 202 206 North, Bridgewater, NJ 08807 0800, USA
    Anal Biochem 323:103-13. 2003
    ..This conformer has a high tendency to aggregate during conventional SDS-polyacrylamide gel electrophoresis (PAGE)...
  88. ncbi Effect of beta-cyclodextrin on the renaturation of enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    Etsushi Yamamoto
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Anal Biochem 381:273-5. 2008
    ..incubation time (several hours) on renaturation of enzymatic activity after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)...
  89. pmc Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc
    K A Montzka
    Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven 06510
    Proc Natl Acad Sci U S A 85:8885-9. 1988
    ..Potential roles for the low-abundance snRNPs in aspects of pre-mRNA processing are discussed...
  90. ncbi Characteristics of human prostasomes isolated from three different sources
    Lena Carlsson
    Department of Medical Sciences, Clinical Chemistry Unit, University Hospital, Uppsala, Sweden
    Prostate 54:322-30. 2003
    ..Prostasomes are organelles secreted by human prostate epithelial cells and appear freely in semen. Most prostasomal functional and biochemical characteristics have been settled on basis of seminal prostasomes (SP)...
  91. ncbi Polyacrylamide gel electrophoresis followed by sodium dodecyl sulfate gradient polyacrylamide gel electrophoresis for the study of the dimer to monomer transition of human transthyretin
    Klaus Altland
    Institut fur Humangenetik, Justus Liebig Universitat, Giessen, Germany
    Electrophoresis 24:2265-71. 2003
    ..We have developed a simple double one-dimensional (D1-D) electrophoretic procedure with polyacrylamide gel electrophoresis (PAGE) followed by sodium dodecylsulfate (SDS) gradient PAGE to study the dimer to monomer ..
  92. ncbi Use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for routine identification of enzymatically digested proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    K L Stone
    HHMI Biopolymer Laboratory and W M Keck Foundation, Yale University, New Haven, CT 06519, USA
    Electrophoresis 19:1046-52. 1998
    ..LC-ESI-MS/MS) analysis of >100 tryptic digests carried out on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separated, Coomassie Blue-stained proteins that were prepared by >50 different ..
  93. ncbi Coupling of native liquid phase isoelectrofocusing and blue native polyacrylamide gel electrophoresis: a potent tool for native membrane multiprotein complex separation
    Gian Maria D'Amici
    Department of Environmental Sciences, Tuscia University, Viterbo, Italy
    J Proteome Res 7:1326-40. 2008
    ..isoelectrofocusing (N-LP-IEF) of protein complexes in the first dimension, followed by blue native polyacrylamide gel electrophoresis (BN-PAGE) in the second dimension, where both the pI and the molecular masses of protein complexes (..
  94. ncbi A multidimensional differential proteomic platform using dual-phase ion-exchange chromatography-polyacrylamide gel electrophoresis/reversed-phase liquid chromatography tandem mass spectrometry
    Andrew K Ottens
    Center for Neuroproteomics and Biomarker Research, University of Florida, Gainesville, Florida 32610, USA
    Anal Chem 77:4836-45. 2005
    ..platform, collectively termed CAX-PAGE/RPLC-MSMS, combines biphasic ion-exchange chromatography with polyacrylamide gel electrophoresis for protein separation, quantification, and differential band targeting, followed by capillary ..
  95. pmc Use of DNA ladders for reproducible protein fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantitative proteomics
    Guoan Zhang
    Department of Pharmacology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York City, New York 10016, USA
    J Proteome Res 7:678-86. 2008
    In proteomics, one-dimensional (1D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein fractionation prior to mass spectrometric analysis to enhance the dynamic range of analysis and to improve ..
  96. pmc High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis
    Tatiana N Laremore
    Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Anal Biochem 401:236-41. 2010
    ..ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment...
  97. ncbi The peripheral stalk participates in the yeast ATP synthase dimerization independently of e and g subunits
    Remi Fronzes
    Institut de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, UMR5095, Universite Victor Segalen Bordeaux 2, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    Biochemistry 45:6715-23. 2006
    ....
  98. ncbi Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation
    Sohini Mukherjee
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 312:1211-4. 2006
    ..The acetylation on MAPKK6 directly competed with phosphorylation, preventing activation of the modified protein. This covalent modification may be used as a general regulatory mechanism in biological signaling...
  99. pmc Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors
    Gustavo A De Souza
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej, Odense M, Denmark
    Genome Biol 7:R72. 2006
    ..Little is known about the protein composition of tear fluid but its deregulation is associated with disease states, such as diabetic dry eyes. This makes this body fluid an interesting candidate for in-depth proteomic analysis...
  100. ncbi Role of the Ada2 and Ada3 transcriptional coactivators in histone acetylation
    Ramakrishnan Balasubramanian
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 1014, USA
    J Biol Chem 277:7989-95. 2002
    ..Our results suggest that Ada2 potentiates the Gcn5 catalytic activity and that Ada3 facilitates nucleosomal acetylation and an expanded lysine specificity...
  101. ncbi Identification of some rabbit allergens as lipocalins
    J Baker
    Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia
    Clin Exp Allergy 31:303-12. 2001
    ..The 18 kDa allergen from saliva may be the previously named rabbit allergen, Ory c 1...

Research Grants62

  1. Controlled delivery of polypeptide hormone calcitonin
    Jagdish Singh; Fiscal Year: 2013
    ..evaluated using Fourier transform infrared spectroscopy, circular dichroism, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, native sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-assisted laser desorption/..
  2. Validation and Quantification of FFPE Antigen Retrieval by Proteome Analysis
    XUPEING FANG; Fiscal Year: 2010
    ..to sample amount constraints imposed by current proteome techniques including two-dimensional polyacrylamide gel electrophoresis and multidimensional liquid chromatography system, the lack of optimized methodologies for ..
  3. Identification of gentamicin-binding proteins in the inner ear
    Takatoshi Karasawa; Fiscal Year: 2010
    ..We will use a gentamicin-agarose pull-down assay and SDS-polyacrylamide gel electrophoresis to isolate such proteins from kidney proximal tubule and cochlear cell lines, and determine their ..
  4. Fast, High-Resolution Protein Separations using Controllable Electro-Osmotic Flow
    FRANK JAHNKE; Fiscal Year: 2010
    ..device that will improve by a factor of 100 upon the performance of traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) used to separation of complex protein mixtures...
  5. Cellular, molecular and functional characterization of proteasomes in EAE
    Oscar A Bizzozero; Fiscal Year: 2013
    ..measure the levels of each complex (20S, 11S- 20S-11S, 19S-20S-19S and 19S-20S-11S) by blue native-polyacrylamide gel electrophoresis and quantitative western blot analysis...
  6. Measuring and monitoring carbon nanotubes and their potential toxicity
    ROCKFORD KEITH DRAPER; Fiscal Year: 2010
    ..We have developed a method that uses sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) for quantifying small amounts of CNTs in liquid samples...
  7. TRANSFORMYLASE ENZYMES DIHYDROFOLATE REDUCTASE
    Stephen J Benkovic; Fiscal Year: 2013
    ..imaging of chimeric fluorescent protein constructs, of co- immunoprecipitation, and of blue native polyacrylamide gel electrophoresis, the latter two linked to MALDI/MS/MS...
  8. Two-Dimensional Electrophoresis Device for Biomarker Detection
    Z Hugh Fan; Fiscal Year: 2010
    ..first dimension (isoelectric focusing) and a number of orthogonal channels for the second dimension (polyacrylamide gel electrophoresis)...
  9. Molecular Micellar-Polyacrylamide Gel Electrophoresis (MoMi-PAGE)
    Isiah M Warner; Fiscal Year: 2010
    ..is to examine better separation strategies for analyses of protein samples using molecular micellar-polyacrylamide gel electrophoresis (MoMi-PAGE). To achieve this objective, this application is divided into three specific aims...
  10. New Materials for High Efficiency Protein Separations
    Mary J Wirth; Fiscal Year: 2011
    ..The separation speeds are limited by the materials. Presently, the workhorse of proteomics is 2D polyacrylamide gel electrophoresis (PAGE)...
  11. Proteomic Profiling of the Vocal Fold Extracellular Matrix
    Nathan Welham; Fiscal Year: 2009
    ..proteomic methodologies: (a) Protein separation by two- dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D SDS-PAGE), and (b) protein species identification by mass spectrometry (MS) fingerprinting, ..
  12. FIBROSIS IN CROHNS DISEASE--ROLE OF IGF I
    ELLEN ZIMMERMANN; Fiscal Year: 1999
    ..PG-PS injected and control rats will be performed by protein analysis of solubilized collagens using polyacrylamide gel electrophoresis and by Northern analysis using collagen specific cDNA probes...
  13. DIARRHEAL DISEASES: CALCIUM AND PHOSPHORYLATION
    GEOFFREY SHARP; Fiscal Year: 1991
    ..All these studies will be performed by incubation with Gamma32-P-ATP and analysis by SDS polyacrylamide gel electrophoresis, autoradiography and densitomery. 2...
  14. PERIPLASMIC FLAGELLA OF TREPONEMA DENTICOLA
    John Ruby; Fiscal Year: 1993
    ..The separation and analysis of PF proteins will be done using sodium dodecyl sulfate- polyacrylamide gel electrophoresis, two dimensional gel electrophoresis, and Western blotting using both polyclonal and monoclonal ..
  15. MATURATION OF LIVER FOR THE METABOLISM OF BILIRUBIN
    Glenn Gourley; Fiscal Year: 1993
    ..1 to 0.5 M KCI gradient. The eluted activator is then isolated by preparative polyacrylamide gel electrophoresis and electroeluted from the gel...
  16. ANTIMICROBIAL EFFECT OF PEROXIDASE IN LEUKOCYTES
    SEYMOUR KLEBANOFF; Fiscal Year: 1999
    ..The organisms will be prelabeled with 59Fe, and polyacrylamide gel electrophoresis, radioautography and histochemical staining will be employed to identify the proteins primarily ..
  17. CARDIAC MUSCLE CELL DEVELOPMENT AND NUCLEAR PROTEINS
    JASWANT BHORJEE; Fiscal Year: 1990
    ..T3 treated and untreated cardiac muscle cells by the high resolution two-dimensional NEPHGE/SDS polyacrylamide gel electrophoresis. High content of HMG 1 and 2 has been correlated to the cell's replicative activity...
  18. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ..nano-materials will be characterized by a number of chemical and bio-analytical techniques including polyacrylamide gel electrophoresis, HPLC, spectroscopy and mass spectrometry...
  19. CHARACTERIZATION OF SERUM FACTORS INDUCING MITOSIS
    HARRY ANTONIADES; Fiscal Year: 1980
    ..chromatography, gel filtration in l M acetic acid, isoelectric focusing and preparative SDS polyacrylamide gel electrophoresis. PDGF has a PI of about 9.8 and a molecular weight of about 13,000 on reduced SDS-PAGE gels...
  20. ERYTHROPOIESIS AND HEMOGLOBIN REGULATION IN MAN
    BLANCHE ALTER; Fiscal Year: 1990
    ..Globin synthesis will be assayed by polyacrylamide gel electrophoresis. Cellular components to be evaluated include monocytes, T cells, and T subsets...
  21. SECOND MESSENGER FUNCTION IN EXOCRINE GLANDS
    Thomas Hurley; Fiscal Year: 1993
    ..b>Polyacrylamide gel electrophoresis and autoradiography will be used to detect and characterize the phosphorylated substrates of kinases ..
  22. MECHANISMS OF BACTERIAL ADHERENCE & COLONIZATION
    DAVID HASTY; Fiscal Year: 1993
    ..The FBGs will be characterized by amino acid analysis, two- dimensional SDS polyacrylamide gel electrophoresis, peptide mapping, and/or immunological crossreactivity...
  23. CONTROL OF CILIA BIOGENESIS IN TETRAHYMENA THERMOPHILA
    David Pennock; Fiscal Year: 1990
    ..basis for cilia paralysis will be determined by comparing motile and paralyzed cilia by 2-D polyacrylamide gel electrophoresis and by electron microscopy...
  24. DEVELOPMENTAL CHANGES IN HEART AND MUSCLE PFK ISOZYMES
    George Dunaway; Fiscal Year: 1991
    ..and amounts of the PFK subunits which are present in the native PFK isozyme pools employing SDS-polyacrylamide gel electrophoresis, immunoblotting or silver staining and quantitative gel scanning...
  25. STUDIES ON SPERM FERTILIZING ABILITY
    Barry Bavister; Fiscal Year: 1993
    ..Factors will be isolated by standard biochemical procedures, including SDS-polyacrylamide gel electrophoresis followed by western blotting, and identified using immunoenzymatic assay...
  26. IONIZING RADIATION MUTAGENESIS IN ESCHERICHIA COLI
    KENDRIC SMITH; Fiscal Year: 1990
    ..in order to identify and characterize their gene products using the maxicell technique and SDS-polyacrylamide gel electrophoresis; (3) to fuse the plasmid-carried xmu genes with a lacZ gene in order to study the regulation of xmu ..
  27. REGULATION OF MITOSIS IN NORMAL AND TRANSFORMED CELLS
    Robert Schlegel; Fiscal Year: 1993
    ..to identify chromosomes that alter normal regulation of mitotic onset; 2) using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to identify specific proteins that are involved in preparing cells for mitosis; 3) ..
  28. INTRACELLULAR TRANSPORT PATHWAYS FOR PROCESSING ANTIGENS
    KATHLEEN MCCOY; Fiscal Year: 1993
    ..Conjugates will be isolated by gel filtration, and their composition will be assessed by polyacrylamide gel electrophoresis. First, the role of these pathways in antigen processing will be determined by the ability of ..
  29. IDENTIFICATION OF BIOCHEMICAL ABNORMALITIES IN PCD CILIA
    LAWRENCE OSTROWSKI; Fiscal Year: 2002
    ..The ciliary proteins will be compared using one- and two-dimensional polyacrylamide gel electrophoresis. Proteins which are altered or absent in the cilia from the PCD cells will be isolated and ..
  30. SIGNAL TRANSDUCTION OF A G PROTEIN COUPLED RECEPTOR
    Athan Kuliopulos; Fiscal Year: 1999
    ..will be retrieved from crude membrane preparations by Ni-chelation chromatography and preparative polyacrylamide gel electrophoresis using techniques established by our group...
  31. TEAR ABNORMALITIES IN NEUROLOGICAL DISEASES
    Patricia Coyle; Fiscal Year: 1990
    ..markers (oligoclonal bands, Ab to myelin basic protein, distinct protein bands) using SDS polyacrylamide gel electrophoresis, immunoblot, and 2-dimensional electrophoresis...
  32. Extreme Halophiles: A Potential Reservoir of New Antibiotics
    Stephen Beckstrom Sternberg; Fiscal Year: 2007
    ..of smaller and smaller pore sizes, gel filtration column chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and reversed-phase and ion exchange high performance liquid chromatography (HPLC)...
  33. SUBSTRATES FOR THE INSULIN RECEPTOR TYROSINE KINASE
    David Lebwohl; Fiscal Year: 1991
    ..by immunoblotting or by immunoprecipitation of labelled proteins, followed by resolution on SDS-polyacrylamide gel electrophoresis. (2) Antibodies prepared to peptides corresponding to the deduced amino acid sequence of the human ..
  34. NOVEL CHARACTERISTICS OF 1-IMIDAZOLE ENZYME INDUCTION
    Michael Franklin; Fiscal Year: 1990
    ..isozymes of cytochrome P-450 will be sought using characteristic monooxygenase activities and SDS polyacrylamide gel electrophoresis of microsomal fractions anion exchange HPLC fractionation, (also coupled with SDS-polyacrylamide gel ..
  35. PROTEINS OF THE HEPATOMA CELL PLASMA MEMBRANE
    DARRELL DOYLE; Fiscal Year: 1991
    ..These proteins then are resolved by two-dimensional polyacrylamide gel electrophoresis in the presence of detergents...
  36. RAPID DETECTION OF CELLULAR PROTEIN DIFFERENCES
    Jonathan Minden; Fiscal Year: 1999
    ..array of cellular proteins is to separate them according to their charge and mass by two-dimensional polyacrylamide gel electrophoresis (2DE), a technique that allows one to visualize approximately 2,000 of the most abundant cellular ..
  37. BLOOD COAGULATION--PLATELET-PLASMA PROTEIN INTERACTION
    ZAVERIO RUGGERI; Fiscal Year: 1990
    ..The subunit structure of normal and vWd plasma vWf was examined by SDS polyacrylamide gel electrophoresis and monoclonal antibody immunoblotting following rapid purification on an analytical scale in the ..
  38. ETHANOL ACTIONS ON NEURONAL MEMBRANE
    PAUL SZE; Fiscal Year: 1990
    ..These binding proteins will be identified by polyacrylamide gel electrophoresis. (3) The third study is to determine by radioimmunoassay the content of calmodulin in the isolated ..
  39. Proteomic and mRNA Profiling of Urine and Urine Sediment
    Brad Rovin; Fiscal Year: 2004
    ..These themes will be developed in two Specific Aims. In Aim 1, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) will be used to map the urine proteins of a well-characterized cohort of patients with SLE ..
  40. PROTEOMIC ANALYSIS OF DROSOPHILA GASTRULATION
    Jonathan Minden; Fiscal Year: 2004
    ..This new technique is a modified two-dimensional polyacrylamide gel electrophoresis method that involves labeling of proteins prior to electrophoresis with differently-colored ..
  41. ANALYSIS OF SPATIAL PATTERNING IN A UNICELLULAR ORGANISM
    Joseph Frankel; Fiscal Year: 1993
    ..A second approach will be to use two-dimensional polyacrylamide gel electrophoresis to detect cell-surface proteins that are absent in janus mutants, and thus might respond to or ..
  42. PLATELET INTERACTIONS WITH FIBRINOGEN
    Ellinor Peerschke; Fiscal Year: 1992
    ..established methods for platelet adhesion, aggregation, secretion, ligand-receptor interactions, SDS-polyacrylamide gel electrophoresis, immunoelectrophoresis, and cell sorting...
  43. MOLECULAR BIOLOGY OF PERIODONTAL LIGAMENT COLLAGENS
    Reginald Taylor; Fiscal Year: 1991
    ..The translation products will be analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate...
  44. STUDY OF HUMAN MULTIPLE MYELOMA CELL ANTIGENS
    Malcolm MacKenzie; Fiscal Year: 1980
    ..labeling followed by reaction with anti-B, T or plasma cell antisera, and the products analyzed by polyacrylamide gel electrophoresis. Antigens will be isolated and characterized by means of potassium chloride extraction gel ..
  45. ALCOHOL & PANCREATIC DIGESTIVE ENZYME SYNTHESIS
    BIDDANDA PONNAPPA; Fiscal Year: 1991
    ....
  46. A 2D-CE System for Rapid and Quantitative Analysis of Clinical Protein Biomarkers
    GARY LOGE; Fiscal Year: 2007
    ..This could be used for routine screening and monitoring for cancer and other diseases in patients, which will allow early treatment to increase survival rate. [unreadable] [unreadable] [unreadable]..
  47. CELL SURFACE INTERACTIONS WITH FIBRONECTIN
    STEVEN AKIYAMA; Fiscal Year: 1990
    ..metabolic labeling methods, immunoaffinity purification of 140K from cell lysates, and detection by polyacrylamide gel electrophoresis and autoradiography...
  48. NOVEL APPROACH TO THE MOLECULAR GENETICS OF PKD1
    Gregory Germino; Fiscal Year: 2003
    ..in affected individuals which can be detected using antisera raised against it by either 1D or 2D polyacrylamide gel electrophoresis. Polyclonal antibodies generated against the peptide products of candidate genes from the PKD1 ..
  49. Microtechnologies Enabling LCM-Based Clinical Proteomics
    Weijie Wang; Fiscal Year: 2006
    ..1-10 microgram. Current proteome technologies, including two-dimensional (2D) polyacrylamide gel electrophoresis and shotgun-based multidimensional liquid chromatography separations, require large cellular samples ..
  50. REGULATION OF CAMP-DEPENDENT PROTEIN KINASES
    Jack Erlichman; Fiscal Year: 1992
    ..Isolated fodrin subunits will be separated by SDS-polyacrylamide gel electrophoresis and transfected to nitrocellulose filters by the Western blot procedure...
  51. THERMODYNAMICS OF BASE PAIR OPENING IN DNA
    Roger Wartell; Fiscal Year: 1993
    ..UV absorbance and polyacrylamide gel electrophoresis will be used to monitor DNA duplex-coil transitions...
  52. LIGANDS FOR ALTERED PHOSPHOPROTEINS IN CANCER
    Kit Lam; Fiscal Year: 2002
    ..Although 2- dimensional polyacrylamide gel electrophoresis was first described in 1975, it is only in the last few years that this technique resurfaced as a ..
  53. CHARACTERIZATION OF CEREBRAL AMYLOID ANGIOPATHIC PEPTIDE
    Harry Vinters; Fiscal Year: 1992
    ..affected cerebral vessel walls, to be examined using isolation of microvessels involved by CAA, SDS polyacrylamide gel electrophoresis of CAA proteins, and their characterization using high performance liquid chromatography, ..
  54. MYOCARDIAL GLYCOPROTEINS IN NORMAL AND DIABETIC STATES
    MARY SPIRO; Fiscal Year: 1992
    ..Radiolabeling experiments and polyacrylamide gel electrophoresis have permitted identification of several matrix glycoproteins (Mr = 200K, 150K, 100K and 70K) of ..
  55. PHOSPHORYLATION OF NEURONAL PROTEINS IN AD
    LEELAVATI MURTHY; Fiscal Year: 1992
    ..of tau and PHF obtained from Western blots and identified by immunoblots from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
  56. TRNA PROCESSING NUCLEASES FROM LIVER MITOCHONDRIA
    GLENN VAN TUYLE; Fiscal Year: 1990
    ..moleuclar sieving techniques, and the multiplicity of protein components will be determined by SDS-polyacrylamide gel electrophoresis. Functional studies of the highly purified enzymes will be performed using normal and altered ..
  57. A BIOCHEMICAL STUDY OF ERROR-PRONE DNA SYNTHESIS
    Kenneth Beattie; Fiscal Year: 1990
    ..High resolution polyacrylamide gel electrophoresis is used to monitor the successive addition of nucleotides onto the 3'-OH terminus of a [5'-32P]..
  58. TWO DIMENSIONAL INTEGRAL ELECTROPHORESIS GEL
    GEORGE LEKA; Fiscal Year: 1999
    ..from applicant's abstract) Since its introduction over twenty years ago, Two dimensional (2D) polyacrylamide gel electrophoresis remains the most powerful and most under utilized approach to purifying the quantities of proteins ..
  59. Two-Dimensional Electrophoresis Gel
    GEORGE LEKA; Fiscal Year: 2002
    Since its introduction over twenty years ago, two dimensional (2D) polyacrylamide gel electrophoresis remains the most powerful and, most under-utilized approach to purifying the quantities of proteins needed for identification and ..
  60. Electronic Protein Transfer for Proteome Analysis
    JESSE BUCH; Fiscal Year: 2005
    ..For the analysis of complex protein mixtures such as cell lysates, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is still the method of choice for separating more than thousands of proteins...
  61. NEUROTOXICOLOGY OF TRIALKYL-TIN AND LEAD COMPOUNDS
    Arthur Haas; Fiscal Year: 1990
    ..on the rates and extent of phosphorylation of endogenous proteins by means of sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography...
  62. Drug Engineering of Transglutaminase 2 Inhibitors
    John Griffin; Fiscal Year: 2009
    ..aims are proposed in this application: 1) Characterize TG2 inhibitors using a non-denaturing polyacrylamide gel electrophoresis (PAGE) assay which distinguishes the closed, inactivated conformation of TG2 from the open, ..