polyacrylamide gel electrophoresis
Summary: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Publications255 found, 100 shown here
- Apoptosis induced by a human milk proteinA Hakansson
Department of Medical Microbiology, Lund University, Sweden
Proc Natl Acad Sci U S A 92:8064-8. 1995..Finally, analysis of the mechanism by which multimeric alpha-lactalbumin induces apoptosis in transformed epithelial cells could lead to the design of antitumor agents...
- Deamidation affects structural and functional properties of human alphaA-crystallin and its oligomerization with alphaB-crystallinRatna Gupta
Department of Physiological Optics, University of Alabama at Birmingham, Birmingham, Alabama 35294 4390, USA
J Biol Chem 279:44258-69. 2004....
- Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon alpha2bSuzanne Hermeling
Central Laboratory Animal Institute, Utrecht University, Utrecht, The Netherlands
Pharm Res 22:1997-2006. 2005..This study was conducted to study the influence of protein structure on the immunogenicity in wild-type and immune tolerant mice of well-characterized degradation products of recombinant human interferon alpha2b (rhIFNalpha2b)...
- Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregationSuzanne Hermeling
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands
J Pharm Sci 95:1084-96. 2006..gel permeation chromatography (GPC), dynamic light scattering (DLS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, and ELISA...
- A sodium dodecyl sulfate-polyacrylamide gel electrophoresis-liquid chromatography tandem mass spectrometry analysis of bovine cartilage tissue response to mechanical compression injury and the inflammatory cytokines tumor necrosis factor alpha and interleAnna L Stevens
Massachusetts Institute of Technology, Cambridge 02139, USA
Arthritis Rheum 58:489-500. 2008....
- Delivery of the non-membrane-permeative antibiotic gentamicin into mammalian cells by using Shigella flexneri membrane vesiclesJ L Kadurugamuwa
Canadian Bacterial Diseases Network, Department of Microbiology, College of Biological Science, University of Guelph, Ontario, Canada
Antimicrob Agents Chemother 42:1476-83. 1998..Being bilayered, with outer faces composed of LPS and Ipa proteins, these MVs were readily engulfed by the otherwise impermeable membranes and eventually liberated their contents into the cytoplasmic substance of the host tissue...
- A novel strategy involved in [corrected] anti-oxidative defense: the conversion of NADH into NADPH by a metabolic networkRanji Singh
Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ontario, Canada
PLoS ONE 3:e2682. 2008....
- Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditionsDaisuke Miyoshi
Frontier Institute for Biomolecular Engineering Research, Konan University, 8 9 1 Okamoto, Higashinada ku, Kobe 658 8501, Japan
J Am Chem Soc 131:3522-31. 2009..2 +/- 0.2 kcal mol(-1), respectively. Furthermore, it was revealed that the opposing effects of molecular crowding on the Hoogsteen and Watson-Crick base pairs were due to different behaviors of water molecules binding to the DNA strands...
- Role of the Ada2 and Ada3 transcriptional coactivators in histone acetylationRamakrishnan Balasubramanian
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 1014, USA
J Biol Chem 277:7989-95. 2002..Our results suggest that Ada2 potentiates the Gcn5 catalytic activity and that Ada3 facilitates nucleosomal acetylation and an expanded lysine specificity...
- Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanismNicolas Rouhier
Unité Mixte de Recherche IaM INRA UHP Nancy I, Biochimie et Biologie Moléculaire Végétales, Universite Henri Poincare, 54506 Vandoeuvre Cedex, France
J Biol Chem 277:13609-14. 2002..Based on these experimental results, a catalytic mechanism is proposed to explain the Grx- and Trx-dependent activities of poplar Prx...
- Two-dimensional blue native/blue native polyacrylamide gel electrophoresis for the characterization of mitochondrial protein complexes and supercomplexesStephanie Sunderhaus
Abt Angewandte Genetik, Naturwissenschafiliche Fakultät, Universitat Hannover, Germany
Methods Mol Biol 372:315-24. 2007Blue native polyacrylamide gel electrophoresis (BN-PAGE) employs the dye Coomassie for the labeling of proteins and protein complexes under native conditions...
- A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposaG Q Zhang
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China
Acta Biochim Pol 56:415-21. 2009..In the case of the lectin from P. aurivella, its antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities have not been determined...
- Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.)Leo van Overbeek
Plant Research International, Wageningen, The Netherlands
FEMS Microbiol Ecol 64:283-96. 2008..Its prevalence was affected by plant growth stage and experiment rather than by plant genotype. It was concluded that plant growth stage overwhelmed any effect of plant genotype on the bacterial communities associated with potato...
- High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresisTatiana N Laremore
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
Anal Biochem 401:236-41. 2010..ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment...
- Coupling of native liquid phase isoelectrofocusing and blue native polyacrylamide gel electrophoresis: a potent tool for native membrane multiprotein complex separationGian Maria D'Amici
Department of Environmental Sciences, Tuscia University, Viterbo, Italy
J Proteome Res 7:1326-40. 2008..isoelectrofocusing (N-LP-IEF) of protein complexes in the first dimension, followed by blue native polyacrylamide gel electrophoresis (BN-PAGE) in the second dimension, where both the pI and the molecular masses of protein complexes (..
- Use of DNA ladders for reproducible protein fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantitative proteomicsGuoan Zhang
Department of Pharmacology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York City, New York 10016, USA
J Proteome Res 7:678-86. 2008In proteomics, one-dimensional (1D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein fractionation prior to mass spectrometric analysis to enhance the dynamic range of analysis and to improve ..
- Polyacrylamide gel electrophoresis followed by sodium dodecyl sulfate gradient polyacrylamide gel electrophoresis for the study of the dimer to monomer transition of human transthyretinKlaus Altland
Institut fur Humangenetik, Justus Liebig Universitat, Giessen, Germany
Electrophoresis 24:2265-71. 2003..We have developed a simple double one-dimensional (D1-D) electrophoretic procedure with polyacrylamide gel electrophoresis (PAGE) followed by sodium dodecylsulfate (SDS) gradient PAGE to study the dimer to monomer ..
- Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plantsWelington L Araujo
Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, Brazil
Appl Environ Microbiol 68:4906-14. 2002..A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC...
- Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysisE Mortz
MDS Proteomics, Odense, Denmark
Proteomics 1:1359-63. 2001..Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry...
- Use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for routine identification of enzymatically digested proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresisK L Stone
HHMI Biopolymer Laboratory and W M Keck Foundation, Yale University, New Haven, CT 06519, USA
Electrophoresis 19:1046-52. 1998..spectrometry (LC-ESI-MS/MS) analysis of >100 tryptic digests carried out on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separated, Coomassie Blue-stained proteins that were prepared by >50 different ..
- A multidimensional differential proteomic platform using dual-phase ion-exchange chromatography-polyacrylamide gel electrophoresis/reversed-phase liquid chromatography tandem mass spectrometryAndrew K Ottens
Center for Neuroproteomics and Biomarker Research, University of Florida, Gainesville, Florida 32610, USA
Anal Chem 77:4836-45. 2005..platform, collectively termed CAX-PAGE/RPLC-MSMS, combines biphasic ion-exchange chromatography with polyacrylamide gel electrophoresis for protein separation, quantification, and differential band targeting, followed by capillary ..
- Critical residues for structure and catalysis in short-chain dehydrogenases/reductasesCharlotta Filling
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden
J Biol Chem 277:25677-84. 2002..This extends the previously recognized catalytic triad of Ser-Tyr-Lys residues to form a tetrad of Asn-Ser-Tyr-Lys in the majority of characterized short-chain dehydrogenases/reductase enzymes...
- Analysis of mitochondrial subunit assembly into respiratory chain complexes using Blue Native polyacrylamide gel electrophoresisMatthew McKenzie
Department of Biochemistry, La Trobe University, Melbourne, VIC 3086, Australia
Anal Biochem 364:128-37. 2007..complexes using radiolabeled, mitochondrially encoded subunits in conjunction with Blue Native polyacrylamide gel electrophoresis. The efficiency of assembly was found to differ markedly between complexes, and intermediate ..
- Characterization of renal damage in canine leptospirosis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting of the urinary proteinsC Zaragoza
Departamento de Medicina y Sanidad Animal, , Facultad de Veterinaria, Universidad de Extremadura, Avda. Universidad s/n, , Spain
J Comp Pathol 129:169-78. 2003..To determine the urine protein pattern in the disease we performed sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on the urine from 10 dogs with leptospirosis and 20 healthy dogs...
- Energetics and cooperativity of tertiary hydrogen bonds in RNA structureS K Silverman
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado at Boulder 80309 0215, USA
Biochemistry 38:8691-702. 1999..4 to -0.5 kcal/mol per tertiary hydrogen bond may be more generally applicable to RNA folding...
- Purification and characterization of a multiprotein component of the Drosophila 26 S (1500 kDa) proteolytic complexA Udvardy
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged
J Biol Chem 268:9055-62. 1993..In chicken, rabbit and human cells a high molecular weight multiprotein complex can be detected, which is immunologically related to the Drosophila mu particle...
- Large-format, two-dimensional polyacrylamide gel electrophoresis of ovine periimplantation uterine luminal fluid proteins: identification of aldose reductase, cytoplasmic actin, and transferrin as conceptus-synthesized proteinsR S Lee
Molecular Embryology AgResearch, Ruakura Research Center, Hamilton, New
Biol Reprod 59:743-52. 1998....
- A novel Bicine running buffer system for doubled sodium dodecyl sulfate - polyacrylamide gel electrophoresis of membrane proteinsTaufika Islam Williams
Department of Chemistry, University of Kentucky, Lexington, KY 40506-0286, USA
Electrophoresis 27:2984-95. 2006..The data clearly indicated that Bicine-dSDS-PAGE is a superior method for the analysis of membrane proteins, providing enhanced resolution and protein representation...
- Structural basis for the inhibition of the essential Plasmodium falciparum M1 neutral aminopeptidaseSheena McGowan
Department of Biochemistry and Molecular Biology and Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria, 3800, Australia
Proc Natl Acad Sci U S A 106:2537-42. 2009..These results set the groundwork for the development of antimalarial therapeutics that target the neutral aminopeptidases of the parasite...
- Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography couRichard C Jones
Divisions of Microbiology, Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079 9502, USA
J Bacteriol 190:5265-78. 2008One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro ..
- A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinaseS Triebel
Department of Biochemistry, Faculty of Chemistry, University of Bielefeld, Germany
FEBS Lett 314:386-8. 1992..The amino acid sequence of tryptic peptides of this protein revealed homology with an alpha 2-microglobulin-related protein from rats, a protein so far unknown in humans...
- N- and C-Terminal motifs in human alphaB crystallin play an important role in the recognition, selection, and solubilization of substratesJoy G Ghosh
Biomolecular Structure and Design, Department of Biological Structure, University of Washington, Seattle, Washington 98195 7420, USA
Biochemistry 45:13847-54. 2006..The results presented in this report established that interactive domains in the N- and C-termini of human alphaB crystallin are important for the recognition, selection, and solubility of unfolding substrate proteins...
- Monoclonal antibody-mediated tumor regression by induction of apoptosisB C Trauth
Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg
Science 245:301-5. 1989..Thus, induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO-1 may be a useful therapeutic approach in treatment of malignancy...
- Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cellsLisa Ott
Friedrich Alexander Universitat Erlangen Nurnberg, Lehrstuhl fur Mikrobiologie, Staudtstr 5, 91058 Erlangen, Germany
BMC Microbiol 10:2. 2010..diphtheriae factors crucial for colonization of the host. In this study, we investigated the function of surface-associated protein DIP1281, previously annotated as hypothetical invasion-associated protein...
- Expression, reconstitution, and structure of an archaeal RNA degrading exosomeEsben Lorentzen
Birkbeck College London, Institute of Structural Molecular Biology, London, United Kingdom
Methods Enzymol 447:417-35. 2008..Furthermore, we describe several crystal structures in which RNA substrates were diffused into crystals and how anomalous scattering from 5-iodo-uridine-modified RNA was used to locate low-occupancy RNA binding sites...
- Potential pathogenic role of aggregative-adhering Corynebacterium diphtheriae of different clonal groups in endocarditisR Hirata Jr
Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciencias Medicas, Universidade do Estado do Rio de Janeiro
Braz J Med Biol Res 41:986-91. 2008..diphtheriae of different clones in endocarditis. Accordingly, the aggregative-adherence pattern may be used as an indication of some invasive potential of C. diphtheriae strains...
- Proteasomal protein degradation in Mycobacteria is dependent upon a prokaryotic ubiquitin-like proteinKristin E Burns
Tuberculosis Research Section, NIAID, NIH, Bethesda, MD 20892, USA
J Biol Chem 284:3069-75. 2009..These data therefore establish that, despite differences in both sequence and target linkage, Pup plays an analogous role to ubiquitin in targeting proteins to the proteasome for degradation...
- GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodiesR P McEver
St Francis Medical Research Institute, University of Oklahoma Health Science Center, Oklahoma City 73104
J Clin Invest 84:92-9. 1989..The restricted expression of GMP-140 in secretory granules of platelets and endothelium suggests that it has a specific function in the vascular system rather than a general role related to inducible secretion...
- Proteomics analysis of nasopharyngeal carcinoma cell secretome using a hollow fiber culture system and mass spectrometryHsin Yi Wu
Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan
J Proteome Res 8:380-9. 2009..We suggest that the HFC system may be used to collect secreted proteins efficiently and facilitate comprehensive characterization of cell secretome...
- The three domains of the mitochondrial outer membrane protein Mim1 have discrete functions in assembly of the TOM complexFranziska Lueder
Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Australia
FEBS Lett 583:1475-80. 2009..In addition, a "late reaction" catalysed by the Sam37 subunit of the SAM complex is also influenced by Mim1. Thus, Mim1 participates at multiple stages in the assembly of the TOM complex...
- Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structureC Lange
, Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt, Germany
EMBO J 20:6591-600. 2001..Site-directed mutagenesis of ligating residues confirmed the importance of the phosphatidylinositol- and cardiolipin-binding sites...
- Preparation of genome-wide DNA fragment libraries using bisulfite in polyacrylamide gel electrophoresis slices with formamide denaturation and quality control for massively parallel sequencing by oligonucleotide ligation and detectionSwati S Ranade
Applied Biosystems, Foster City, CA 94404, USA
Anal Biochem 390:126-35. 2009....
- Inositol pyrophosphates and their unique metabolic complexity: analysis by gel electrophoresisOriana Losito
Department of Cell and Developmental Biology, Medical Research Council Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London, London, United States of America
PLoS ONE 4:e5580. 2009..Though likely involved in a wide range of cellular functions, the study of inositol pyrophosphates has suffered from a lack of readily available methods for their analysis...
- Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial diseaseAnn Saada
Metabolic Disease Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Am J Hum Genet 84:718-27. 2009..These data not only show that NDUFAF3 mutations cause complex I deficiency but also relate different complex I disease genes by the close cooperation of their encoded proteins during the assembly process...
- Roles of a membrane-bound caleosin and putative peroxygenase in biotic and abiotic stress responses in ArabidopsisMark Partridge
Biotechnology Unit, Division of Biological Sciences, University of Glamorgan, Treforest, CF37 1DL, United Kingdom
Plant Physiol Biochem 47:796-806. 2009..We propose that Clo-3 is part of an oxylipin pathway induced by multiple stresses and may also generate fatty acid derived anti-fungal compounds for plant defence...
- A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptideC R R Ramos
Centro de Biotecnologia, Instituto Butantan, , SP, Brazil
Braz J Med Biol Res 37:1103-9. 2004..Thus, although proteins expressed by pRSET plasmids also have a hexa-histidine tag, the fusion peptide is much longer and may represent a problem for some recombinant proteins...
- The beta-barrel outer membrane protein assembly complex of Neisseria meningitidisElena B Volokhina
Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
J Bacteriol 191:7074-85. 2009..Thus, the Bam complex in N. meningitidis consists of Omp85/BamA plus RmpM, BamC, ComL/BamD, and BamE, of which ComL/BamD and BamE appear to be the most important accessory components for OMP assembly...
- HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative BimDan Chen
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3206, USA
EMBO J 21:6801-10. 2002..Our findings reveal a strategy by which Tat induces apoptosis by targeting the microtubule network. Thus HIV-1 Tat joins a growing list of pathogen-derived proteins that target the cytoskeleton of host cells...
- The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factorsA J Ridley
Institute for Cancer Research, Chester Beatty Laboratories, London, England
Cell 70:389-99. 1992..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors...
- Electrophoresis techniques to investigate defects in oxidative phosphorylationMaria Antonietta Calvaruso
Nijmegen Center for Mitochondrial Disorders at the Department of Pediatrics, Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
Methods 46:281-7. 2008..Here we provide a practical guide on how to set-up the basic technique to study OXPHOS defects in patient-derived cells and tissues...
- Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresisEmiko Kinoshita Kikuta
Department of Functional Molecular Science, Graduate School of Biomedical Sciences, Kasumi 1 2 3, Hiroshima University, Hiroshima 734 8553, Japan
Mol Cell Proteomics 6:356-66. 2007..describe three applications of label-free kinase profiling using a novel type of phosphate affinity polyacrylamide gel electrophoresis. The phosphate affinity site is a polyacrylamide-bound dinuclear Mn2+ complex that enables the ..
- Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expressionA T Behan
Department of Psychiatry, Royal College of Surgeons in Ireland, RCSI ERC, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland
Mol Psychiatry 14:601-13. 2009..methods, two-dimensional difference gel electrophoresis and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by reverse phase-liquid chromatography-tandem mass spectrometry (RP-LC-MS/MS) (gel ..
- Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresisJerri Ross
Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8026, USA
J Clin Virol 42:374-80. 2008....
- A unique caleosin in oil bodies of lily pollenPei Luen Jiang
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan
Plant Cell Physiol 49:1390-5. 2008..Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies...
- Changes in ferritin H- and L-chains in canine lenses with age-related nuclear cataractMalgorzata Goralska
Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, North Carolina 27606, USA
Invest Ophthalmol Vis Sci 50:305-10. 2009..To determine potential differences in the characteristics of the iron storage protein ferritin and its heavy (H) and light (L) subunits in fiber cells from cataractous and noncataractous lenses of older dogs...
- Sequence effects in single-base loops for quadruplexesAurore Guédin
INSERM, U565, Acides nucléiques dynamique, ciblage et fonctions biologiques, 43 rue Cuvier, CP26, F 75231 Paris Cedex 05, France
Biochimie 90:686-96. 2008....
- A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresisChun Yi Liau
Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, R O C Department of Bioengineering, Tatung University, Taipei 104, Taiwan, ROC
J Biosci Bioeng 106:111-3. 2008..Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme...
- Gender difference in the composition of fecal flora in laboratory mice, as detected by denaturing gradient gel electrophoresis (DGGE)Seigo Fushuku
Center for Laboratory Animal Science, National Defense Medical College, Saitama, Japan
Exp Anim 57:489-93. 2008..Male and female mice were distributed on opposite sides of the origin of the plane in NMDS at weeks 0, 2, 3, and 8. These results suggest a gender difference in the composition of intestinal flora in mice...
- Histone H4 N-terminal acetylation in Kasumi-1 cells treated with depsipeptide determined by acetic acid-urea polyacrylamide gel electrophoresis, amino acid coded mass tagging, and mass spectrometryLiwen Zhang
Mass Spectrometry and Proteomics Facility, Campus Chemical Instrument Center, Department of Chemistry, Division of Hematology Oncology, The Ohio State University, Columbus, Ohio 43210, USA
J Proteome Res 6:81-8. 2007..We describe here an analytical approach that combines acid urea polyacrylamide gel electrophoresis (AU-PAGE), amino acid coded mass tagging (AACM), and mass spectrometry (MS) for the investigation of ..
- An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small moleculesYuan Yao
Laboratory of Biochemistry and Biophysics, College of Life Sciences, Wuhan University, Wuhan 430072, People s Republic of China
Nucleic Acids Res 35:e68. 2007..Here, we describe an exonuclease I hydrolysis assay that evaluates quadruplex stabilization by DNA-interacting compounds, discriminates inhibitory effect from different sources and helps determine the optimal compound concentration...
- Alanine scanning and Fe-BABE probing of the bacteriophage ø29 prohead RNA-connector interactionRockney Atz
Department of Diagnostic and Biological Sciences, University of Minnesota, Minneapolis, MN 55455, USA
J Mol Biol 369:239-48. 2007....
- Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virensK Feng
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing
Biochem Biophys Res Commun 345:1573-8. 2006....
- Important contributions of a new quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE) procedure for elucidating metal cofactor metabolisms in protein-misfolding diseases--a theoryBernd Kastenholz
Institute for Chemistry and Dynamics of the Geosphere, Institute III Phytosphere, Research Centre Juelich, Juelich 52425, Germany
Protein Pept Lett 13:503-8. 2006..purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis procedure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially ..
- Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometryCaroline Vipond
Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
Hum Vaccin 1:80-4. 2005..One dimensional polyacrylamide gel electrophoresis and semi-quantitative measurement of the major proteins is one method used to evaluate and control ..
- Altered proteolytic activities of ADAMTS-4 expressed by C-terminal processingMasahide Kashiwagi
Kennedy Institute of Rheumatology Division, Imperial College London, United Kingdom
J Biol Chem 279:10109-19. 2004..Finding of ADAMTS-4 in the interleukin-1alpha-treated porcine articular cartilage primarily as a 46-kDa form suggests that it exhibits a broader substrate spectrum in the tissue than originally considered...
- Functional domains of Brevibacillus thermoruber lon protease for oligomerization and DNA binding: role of N-terminal and sensor and substrate discrimination domainsAlan Yueh-Luen Lee
Institute of Biological Chemistry, Academia Sinica, Taipei 115
J Biol Chem 279:34903-12. 2004..The results also showed that hydrophobic interactions may play important roles in the dimerization of Bt-Lon, and ionic interactions are mainly responsible for the assembly of hexamers...
- Molecular cloning of goat 20alpha-hydroxysteroid dehydrogenase cDNAWalimuni Samantha Nilanthi Jayasekara
Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Japan
J Reprod Dev 50:323-31. 2004..The present study suggests that goat 20alpha-HSD is another member of the aldo-keto reductase superfamily and that it plays a role in the placenta during pregnancy...
- Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterizationTakeshi Ishimizu
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
J Biol Chem 279:38555-62. 2004..Indeed, AtEBM-like genes are only found in plants, indicating that endo-beta-mannosidase is a plant-specific enzyme. The role of this enzyme in the processing and/or degradation of N-glycan will be discussed...
- Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1betaDavide Comoletti
Department of Pharmacology, University of California, La Jolla, California 92093 0636, USA
J Biol Chem 278:50497-505. 2003..We show here that glycosylation processing of neuroligin, in addition to mRNA splicing and gene selection, contributes to the specificity of the neurexin-beta/neuroligin-1 association...
- On-column refolding and characterization of soluble human interleukin-15 receptor alpha-chain produced in Escherichia coliMitsuhiro Matsumoto
Pharmaceuticals and Biotechnology Laboratory, Japan Energy Corporation, 3-17-35 Niizo-Minami, Toda, 335-8502, Saitama, Japan
Protein Expr Purif 31:64-71. 2003..Thus, we succeeded in refolding shIL-15Ralpha to an active form on an affinity column...
- Functional roles of four conserved charged residues in the membrane domain subunit NuoA of the proton-translocating NADH-quinone oxidoreductase from Escherichia coliMou Chieh Kao
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 279:32360-6. 2004..The possible functional roles of residues Asp-79 and Glu-81 were discussed...
- Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich regionJung Mao Hsu
Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei 115, Taiwan
J Biol Chem 279:32592-602. 2004..Taken together, these findings further expound the diverse substrate-recognition abilities of the SCF complexes...
- The oligomerization state determines regulatory properties and inhibitor sensitivity of type 4 cAMP-specific phosphodiesterasesWito Richter
Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California 94305 5317, USA
J Biol Chem 279:30338-48. 2004..Taken together, our data indicate that dimerization defines the properties of PDE4 enzymes and suggest a common structural and functional organization for all PDEs...
- A Kazal-like extracellular serine protease inhibitor from Phytophthora infestans targets the tomato pathogenesis-related protease P69BMiaoying Tian
Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA
J Biol Chem 279:26370-7. 2004..In addition, this study points to a common virulence strategy between the oomycete plant pathogen P. infestans and several mammalian parasites, such as the apicomplexan Toxoplasma gondii...
- Tom1, a VHS domain-containing protein, interacts with tollip, ubiquitin, and clathrinMegumi Yamakami
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
J Biol Chem 278:52865-72. 2003..Thus, we propose that a Tom1-Tollip complex functions as a factor that links polyubiquitinated proteins to clathrin...
- Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptationYutaka Suzuki
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Japan
Eur J Biochem 271:1372-81. 2004..20 degrees C, and total soluble proteins extracted from the cells were analyzed by two-dimensional polyacrylamide gel electrophoresis. Comparison of these patterns showed that the cellular content of a protein with a molecular mass of ..
- Mutagenesis analysis of the interaction between the dorsal rel homology domain and HMG boxes of DSP1 proteinDavy Martin
Centre de Biophysique Moleculaire, CNRS, conventionné avec l Université d Orléans, rue Charles Sadron, 45071 Orleans Cedex 2, France
J Biochem 134:583-9. 2003....
- Purification and characterization of a barley aleurone abscisic acid-binding proteinFawzi A Razem
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
J Biol Chem 279:9922-9. 2004..Our data show that ABAP1 exerts high binding affinity for ABA. The interaction is reversible, follows saturation kinetics, and has stereospecificity, thus meeting the criteria for an ABA-binding protein...
- Functional analysis of multiple single-stranded DNA-binding proteins from Methanosarcina acetivorans and their effects on DNA synthesis by DNA polymerase BIJustin B Robbins
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
J Biol Chem 279:6315-26. 2004..Although bacterial SSB and eukaryotic RPA have been shown to stimulate DNA synthesis by their cognate DNA polymerases, our findings provide the first in vitro biochemical evidence for the conservation of this property in an archaeon...
- Specialized roles of the two pathways for the synthesis of mannosylglycerate in osmoadaptation and thermoadaptation of Rhodothermus marinusNuno Borges
, Universidade Nova de Lisboa, Oeiras, Portugal
J Biol Chem 279:9892-8. 2004..marinus to two different types of aggression. This is the only example of pathway multiplicity being rationalized in terms of the need to respond efficiently to distinct environmental stresses...
- The pyruvate formate lyase family: sequences, structures and activationL Lehtiö
Graduate School in Informational and Structural Biology, Institute of Biotechnology, University of Helsinki, PO Box 65, FIN 00014 Helsinki, Finland
Protein Eng Des Sel 17:545-52. 2004..Surprisingly, most of the conserved residues in PFL-like enzymes appear to be involved in preserving the structure, rather than forming the active site...
- The Pro335 --> Leu polymorphism of type 3 inositol 1,4,5-trisphosphate receptor found in mouse inbred lines results in functional changeSanghyeon Kim
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yusong gu, Daejeon
J Biol Chem 280:26024-31. 2005..The results indicate that the polymorphism of IP3R3 causes changes in receptor function, presumably affecting intracellular Ca2+ signaling...
- The intertidal copepod Tigriopus japonicus small heat shock protein 20 gene (Hsp20) enhances thermotolerance of transformed Escherichia coliJung Soo Seo
Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, Republic of Korea
Biochem Biophys Res Commun 340:901-8. 2006..These findings provide a better understanding of cellular protection mechanisms against environmental stress such as heat shock...
- Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinasesHiroshi Tokumitsu
Department of Signal Transduction Sciences, Faculty of Medicine, Kagawa University, 1750 1 Miki cho, Kita gun, Kagawa 761 0793, Japan
J Biol Chem 280:35108-18. 2005..Taken together, these results indicate that the Numb family proteins may be intracellular targets for CaM-Ks, and they may also be regulated by phosphorylation-dependent interaction with 14-3-3 protein...
- Identification of a novel secreted protease from Pseudomonas aeruginosa that causes corneal erosionsMary E Marquart
Department of Microbiology, University of Mississippi Medical Center, Jackson, 39216, USA
Invest Ophthalmol Vis Sci 46:3761-8. 2005..CONCLUSIONS: PASP is a protease that has not been previously identified. It causes corneal epithelial erosions, indicating its likely activity as a virulence-promoting factor in Pseudomonas keratitis...
- Production of recombinant human trimeric CD137L (4-1BBL). Cross-linking is essential to its T cell co-stimulation activityCatherine Rabu
INSERM U601, 9 quai Moncousu, 44035 Nantes Cedex, France
J Biol Chem 280:41472-81. 2005..In addition, we show that the cross-linking of two trimeric AviTag-4-1BBL molecules was the minimum step required to elicit significant costimulatory activity...
- AtKP1, a kinesin-like protein, mainly localizes to mitochondria in Arabidopsis thalianaCheng Zhi Ni
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing
Cell Res 15:725-33. 2005..The AtKP1 protein could not be released following a 0.6 M KI washing, indicating that AtKP1 is tightly bind to mitochondria and might function associated with this kind of organelles...
- Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitorSomchai Chutipongtanate
Medical Molecular Biology Unit, Office for Research and Development, Siriraj Hospital, Mahidol University, Bangkok, Thailand
J Clin Invest 115:3613-22. 2005..These data indicate that TFF1 is a novel potent CaOx crystal growth inhibitor with a potential pathophysiological role in nephrolithiasis...
- Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientationTakaki Koide
Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 950 2081, Japan
J Biol Chem 281:3432-8. 2006..R., Della, M., and Miller, A. D. (2001) J. Biol. Chem. 276, 49310-49319)...
- Purification, characterization, cloning and expression of pyruvate decarboxylase from Torulopsis glabrata IFO005Qinhong Wang
Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P.R. China
J Biochem (Tokyo) 136:447-55. 2004..They also exhibited the similar catalytic properties: low thermostability, similar pH stability and optimal pH, and complete inhibition by glyoxylate...
- A novel protein derived from the MUC1 gene by alternative splicing and frameshiftingFiana Levitin
Department of Cell Research and Immunology, Tel Aviv University, Ramat Aviv 69978, Israel
J Biol Chem 280:10655-63. 2005....
- Clumping factor B, a fibrinogen-binding MSCRAMM (microbial surface components recognizing adhesive matrix molecules) adhesin of Staphylococcus aureus, also binds to the tail region of type I cytokeratin 10Evelyn J Walsh
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland
J Biol Chem 279:50691-9. 2004..We provide an explanation for the molecular basis for S. aureus adherence to the squamous epithelium and suggest that nasal colonization might be prevented by reagents that inhibit this interaction...
- Expression and characterization of a recombinant unique acid phosphatase from kidney bean hypocotyl exhibiting chloroperoxidase activity in the yeast Pichia pastorisTohru Yoneyama
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099 2493, Japan
Protein Expr Purif 53:31-9. 2007..This expression system produces sufficient protein to allow us to attempt to determine the three-dimensional crystal structure, which will shed light on its unique mechanism of converting KhACP to vanadate-dependent chloroperoxidase...
- A novel 43-kDa protein as a negative regulatory component of phenoloxidase-induced melanin synthesisMingyi Zhao
National Research Laboratory of Defense Proteins, College of Pharmacy, Pusan National University, Jangjeon Dong, Kumjeong Ku, Busan 609-735, Korea
J Biol Chem 280:24744-51. 2005..These results suggest that this 43-kDa MIP inhibits the formation of melanin and thus is a modulator of the melanization reaction to prevent the insect from excessive melanin synthesis in places where it should be inappropriate...
- Porcine purple acid phosphatase: heterologous expression, characterization, and proteolytic analysisJoseph Itor Naseri
Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, 48149 Munster, Germany
Arch Biochem Biophys 432:25-36. 2004..Digestion with the aspartic protease pepsin resulted in a 2.5-fold enzyme inactivation correlated with the appearance of low molecular weight polypeptide fragments and the release of enzyme-bound iron...
- Mitochondrial trans-2-enoyl-CoA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesisMeike Hoffmeister
Institute of Botany III, , , Germany
J Biol Chem 280:4329-38. 2005..Trans-2-enoyl-CoA reductase assigns a biochemical activity, NAD(P)H-dependent acyl-CoA synthesis from enoyl-CoA, to one member of this gene family of previously unknown function...
- Deletion and purification studies to elucidate the structure of the Actinobacillus actinomycetemcomitans cytolethal distending toxinKeitarou Saiki
Department of Microbiology, Nippon Dental University School of Dentistry at Tokyo, Chiyoda ku, Tokyo 102 8159, Japan
J Biochem 136:335-42. 2004..Furthermore, this study describes an effective protocol for the purification of a native rather than reconstituted CDT, and clarifies the subunit composition of the active CDT holotoxin...
- Expression, purification and biochemical characterization of a single-stranded DNA binding protein from Herbaspirillum seropedicaeJavier Vernal
Laboratório de Expressão Gênica, Departamento de Bioquimica, Universidade Federal de Santa Catarina, 88040 900 Florianopolis, SC, Brazil
Protein Expr Purif 53:195-200. 2007..The production of this recombinant protein in good yield opens up the possibility of obtaining its 3D-structure and will help further investigations into DNA metabolism...
- Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zincSeiya Watanabe
Institute of Advanced Energy, Kyoto University, Gokasyo, Uji, Kyoto 611-0011, Japan
J Biol Chem 280:10340-9. 2005..The introduction of this mutation in D207A/I208R/F209S and D207A/I208R/F209S/N211R mutants increased the thermostability and further increased the catalytic activity with NADP(+)...
- Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insightsMartin Gengenbacher
, , Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany
J Biol Chem 281:3633-41. 2006..62 A. The obstruction of the oxyanion hole indicates that Pdx2 is in a resting state and that activation occurs upon Pdx1-Pdx2 complex formation...
- Expression of normal and abnormal porcine kidney D-amino acid oxidase in Escherichia coli: purification and characterization of the enzymesF Watanabe
Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka, Japan
Biochem Biophys Res Commun 165:1422-7. 1989..The use of a GTG triplet in the 5'-untranslated region of DAO cDNA as the initiation codon for the synthesis of the abnormal DAO is suggested...
- BIOPHYSICS AND GENETICS OF VIRAL DNA PACKAGINGPhilip Serwer; Fiscal Year: 1992..binding of fluorescent structure probes, protein-protein crosslinking, sodium dodecyl sulfate polyacrylamide gel electrophoresis and protease digestion will be used; (d) isolate new DNA packaging intermediates; packaging will be ..
- MOLECULAR STRUCTURE/FUNCTION OF TEKTINS IN MICROTUBULESRICHARD LINCK; Fiscal Year: 1993..Finally, using the cDNA probes, the expression and role of tektins in sea urchin embryonic ciliogenesis will be investigated...
- EFFECT OF MARIJUANA ON MACROPHAGE ANTIGEN PRESENTATIONGuy Cabral; Fiscal Year: 1993..14C]-amino acids, or [3H]-glucosamine, and subjected to one-dimensional or two-dimensional isodalt polyacrylamide gel electrophoresis in concert within radioautography, will be performed to establish the effect of THC on the time and ..
- NUCLEAR PROTEIN CONTENT AND HEAT-INDUCED CELL KILLINGJoseph Roti Roti; Fiscal Year: 1993..This aim will be accomplished by analyzing nuclear proteins by polyacrylamide gel electrophoresis (PAGE) immediately after various heat exposures. 3...