polyacrylamide gel electrophoresis

Summary

Summary: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

Top Publications

  1. ncbi Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry
    Rainy Mears
    Cancer Research UK Clinical Centre, St James s University Hospital, Leeds, UK
    Proteomics 4:4019-31. 2004
  2. ncbi Identification, proteomic profiling, and origin of ram epididymal fluid exosome-like vesicles
    Jean Luc Gatti
    Equipe Gamètes Males et Fertilité, URM 6175 INRA CNRS Université de TOURS Haras Nationaux, Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique de Nouzilly, Monnaie 37380, France
    Biol Reprod 72:1452-65. 2005
  3. pmc Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography cou
    Richard C Jones
    Divisions of Microbiology, Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079 9502, USA
    J Bacteriol 190:5265-78. 2008
  4. ncbi A proteomic analysis of human bile
    Troels Zakarias Kristiansen
    McKusick Nathans Institute of Genetic Medicine and Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Cell Proteomics 3:715-28. 2004
  5. doi Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.)
    Leo van Overbeek
    Plant Research International, Wageningen, The Netherlands
    FEMS Microbiol Ecol 64:283-96. 2008
  6. pmc Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
    C Lange
    , Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt, Germany
    EMBO J 20:6591-600. 2001
  7. ncbi A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
    C R R Ramos
    Centro de Biotecnologia, Instituto Butantan, Sao Paulo, SP, Brazil
    Braz J Med Biol Res 37:1103-9. 2004
  8. pmc The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition
    W G Whitfield
    Department of Biochemistry, The University, Dundee, Scotland
    EMBO J 9:2563-72. 1990
  9. pmc Apoptosis induced by a human milk protein
    A Hakansson
    Department of Medical Microbiology, Lund University, Sweden
    Proc Natl Acad Sci U S A 92:8064-8. 1995
  10. pmc Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota
    Inés Martínez
    Department of Food Science and Technology, University of Nebraska, Lincoln, 68583 0919, USA
    Appl Environ Microbiol 75:4175-84. 2009

Research Grants

Detail Information

Publications297 found, 100 shown here

  1. ncbi Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry
    Rainy Mears
    Cancer Research UK Clinical Centre, St James s University Hospital, Leeds, UK
    Proteomics 4:4019-31. 2004
    ..The two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) protein profiles of exosomes from the two cell lines were highly comparable and ..
  2. ncbi Identification, proteomic profiling, and origin of ram epididymal fluid exosome-like vesicles
    Jean Luc Gatti
    Equipe Gamètes Males et Fertilité, URM 6175 INRA CNRS Université de TOURS Haras Nationaux, Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique de Nouzilly, Monnaie 37380, France
    Biol Reprod 72:1452-65. 2005
    ..The main membrane-linked proteins of the vesicles were not retrieved in the extract from cauda or ejaculated sperm, suggesting that these vesicles did not fuse with sperm in vivo...
  3. pmc Relative quantitative comparisons of the extracellular protein profiles of Staphylococcus aureus UAMS-1 and its sarA, agr, and sarA agr regulatory mutants using one-dimensional polyacrylamide gel electrophoresis and nanocapillary liquid chromatography cou
    Richard C Jones
    Divisions of Microbiology, Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079 9502, USA
    J Bacteriol 190:5265-78. 2008
    One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro ..
  4. ncbi A proteomic analysis of human bile
    Troels Zakarias Kristiansen
    McKusick Nathans Institute of Genetic Medicine and Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA
    Mol Cell Proteomics 3:715-28. 2004
    ..In combination with "tagging" approaches for differential proteomics, our method could be used for identification of cancer biomarkers from any body fluid...
  5. doi Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.)
    Leo van Overbeek
    Plant Research International, Wageningen, The Netherlands
    FEMS Microbiol Ecol 64:283-96. 2008
    ..Its prevalence was affected by plant growth stage and experiment rather than by plant genotype. It was concluded that plant growth stage overwhelmed any effect of plant genotype on the bacterial communities associated with potato...
  6. pmc Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
    C Lange
    , Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt, Germany
    EMBO J 20:6591-600. 2001
    ..Site-directed mutagenesis of ligating residues confirmed the importance of the phosphatidylinositol- and cardiolipin-binding sites...
  7. ncbi A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
    C R R Ramos
    Centro de Biotecnologia, Instituto Butantan, Sao Paulo, SP, Brazil
    Braz J Med Biol Res 37:1103-9. 2004
    ..Thus, although proteins expressed by pRSET plasmids also have a hexa-histidine tag, the fusion peptide is much longer and may represent a problem for some recombinant proteins...
  8. pmc The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition
    W G Whitfield
    Department of Biochemistry, The University, Dundee, Scotland
    EMBO J 9:2563-72. 1990
    ..These observations suggest that cyclin A has a role in the G2-M transition that is independent of spindle formation, and that entry into anaphase is a key requirement for the degradation of cyclin B...
  9. pmc Apoptosis induced by a human milk protein
    A Hakansson
    Department of Medical Microbiology, Lund University, Sweden
    Proc Natl Acad Sci U S A 92:8064-8. 1995
    ..Finally, analysis of the mechanism by which multimeric alpha-lactalbumin induces apoptosis in transformed epithelial cells could lead to the design of antitumor agents...
  10. pmc Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota
    Inés Martínez
    Department of Food Science and Technology, University of Nebraska, Lincoln, 68583 0919, USA
    Appl Environ Microbiol 75:4175-84. 2009
    ..This study provides evidence that modulation of the gut microbiota-host metabolic interrelationship by dietary intervention has the potential to improve mammalian cholesterol homeostasis, which has relevance for cardiovascular health...
  11. pmc A systematic characterization of mitochondrial proteome from human T leukemia cells
    Karim Rezaul
    Department of Cell Biology, Center for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Mol Cell Proteomics 4:169-81. 2005
    ..Thus, we conclude that this approach is suitable for characterizing subcellular proteomes form multiple cells and tissues...
  12. ncbi Analysis of the subunit composition of complex I from bovine heart mitochondria
    Joe Carroll
    Medical Research Council Dunn Human Nutrition Unit, The Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    Mol Cell Proteomics 2:117-26. 2003
    ..It is unlikely that any more subunits of the bovine complex remain undiscovered. Therefore, the intact enzyme is a complex of 46 subunits, and, assuming there is one copy of each subunit in the complex, its mass is 980 kDa...
  13. ncbi Preparation of coated nanoparticles for a new mucosal vaccine delivery system
    Olga Borges
    Leiden Amsterdam Center for Drug Research, Division of Pharmaceutical Technology, P O Box 9502, 2300 RA Leiden, The Netherlands
    Int J Pharm 299:155-66. 2005
    ..The optimisation of the coating process resulted in 35% (w/w) for the loading capacity of the coated particles. SEM investigations confirmed a suitable size of the coated nanoparticles for the uptake by M-cells...
  14. pmc The many applications of acid urea polyacrylamide gel electrophoresis to studies of tRNAs and aminoacyl-tRNA synthetases
    Caroline Köhrer
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 68 671, Cambridge, MA 02139, USA
    Methods 44:129-38. 2008
    Here we describe the many applications of acid urea polyacrylamide gel electrophoresis (acid urea PAGE) followed by Northern blot analysis to studies of tRNAs and aminoacyl-tRNA synthetases...
  15. ncbi Catalogue of soluble proteins in human vitreous humor by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization mass spectrometry including seven angiogenesis-regulating factors
    Reiko Koyama
    Department of Ophthalmology, Osaka Medical College, 2 7 Daigakucho, Takatsuki, Osaka 569 8686, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 792:5-21. 2003
    ..To improve the recovery of proteins in vitreous, we applied one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-PAGE)...
  16. ncbi Deamidation affects structural and functional properties of human alphaA-crystallin and its oligomerization with alphaB-crystallin
    Ratna Gupta
    Department of Physiological Optics, University of Alabama at Birmingham, Birmingham, Alabama 35294 4390, USA
    J Biol Chem 279:44258-69. 2004
    ....
  17. ncbi Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis
    E Mortz
    MDS Proteomics, Odense, Denmark
    Proteomics 1:1359-63. 2001
    ..Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry...
  18. pmc GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies
    R P McEver
    St Francis Medical Research Institute, University of Oklahoma Health Science Center, Oklahoma City 73104
    J Clin Invest 84:92-9. 1989
    ..The restricted expression of GMP-140 in secretory granules of platelets and endothelium suggests that it has a specific function in the vascular system rather than a general role related to inducible secretion...
  19. ncbi Analysis of avocado allergen (Prs a 1) IgE-binding peptides generated by simulated gastric fluid digestion
    Araceli Diaz-Perales
    Departamento de Biotecnologia, E T S Ingenieros Agronomos, UPM, Madrid, Spain
    J Allergy Clin Immunol 112:1002-7. 2003
    ..Allergenic plant class I chitinases, such as avocado Prs a 1, are the panallergens involved in the latex-fruit syndrome. Previous reports indicated their susceptibility to simulated gastric fluid (SGF) digestion...
  20. ncbi Structural characterization and immunogenicity in wild-type and immune tolerant mice of degraded recombinant human interferon alpha2b
    Suzanne Hermeling
    Central Laboratory Animal Institute, Utrecht University, Utrecht, The Netherlands
    Pharm Res 22:1997-2006. 2005
    ..This study was conducted to study the influence of protein structure on the immunogenicity in wild-type and immune tolerant mice of well-characterized degradation products of recombinant human interferon alpha2b (rhIFNalpha2b)...
  21. ncbi Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregation
    Suzanne Hermeling
    Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
    J Pharm Sci 95:1084-96. 2006
    ..gel permeation chromatography (GPC), dynamic light scattering (DLS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting, and ELISA...
  22. ncbi A bacterially expressed particulate hepatitis E vaccine: antigenicity, immunogenicity and protectivity on primates
    Shao W Li
    The Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
    Vaccine 23:2893-901. 2005
    ..The efficacy of the vaccine was essentially the same for the homologous genotype 1 virus and heterologous genotype 4 virus...
  23. pmc Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants
    Welington L Araujo
    Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Piracicaba, Brazil
    Appl Environ Microbiol 68:4906-14. 2002
    ..A specific band with a high G+C ratio was observed only in asymptomatic plants. The higher frequency of C. flaccumfaciens in asymptomatic plants suggests a role for this organism in the resistance of plants to CVC...
  24. ncbi Design and self-assembly of two-dimensional DNA crystals
    E Winfree
    Computation and Neural Systems, California Institute of Technology, Pasadena 91125, USA
    Nature 394:539-44. 1998
    ..The patterned crystals have been visualized by atomic force microscopy...
  25. pmc Subinhibitory concentrations of linezolid reduce Staphylococcus aureus virulence factor expression
    Katussevani Bernardo
    Institute for Medical Microbiology, Immunology, and Hygiene, Medical Center, University of Cologne, Cologne, Germany
    Antimicrob Agents Chemother 48:546-55. 2004
    ..on the secretion of exotoxins by Staphylococcus aureus was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry and ..
  26. ncbi Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei
    Narisara Anuntagool
    Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand
    Microbiol Immunol 46:143-50. 2002
    ..e., the presence or absence of exopolysaccharide and the types of lipopolysaccharide. The heterogeneity of the LPS from these two closely related organisms is most likely related to the differences in its O-polysaccharide side chain...
  27. pmc A novel strategy involved in [corrected] anti-oxidative defense: the conversion of NADH into NADPH by a metabolic network
    Ranji Singh
    Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ontario, Canada
    PLoS ONE 3:e2682. 2008
    ....
  28. doi A sodium dodecyl sulfate-polyacrylamide gel electrophoresis-liquid chromatography tandem mass spectrometry analysis of bovine cartilage tissue response to mechanical compression injury and the inflammatory cytokines tumor necrosis factor alpha and interle
    Anna L Stevens
    Massachusetts Institute of Technology, Cambridge 02139, USA
    Arthritis Rheum 58:489-500. 2008
    ....
  29. doi Two-dimensional blue native/blue native polyacrylamide gel electrophoresis for the characterization of mitochondrial protein complexes and supercomplexes
    Stephanie Sunderhaus
    Abt Angewandte Genetik, Naturwissenschafiliche Fakultät, Universitat Hannover, Germany
    Methods Mol Biol 372:315-24. 2007
    Blue native polyacrylamide gel electrophoresis (BN-PAGE) employs the dye Coomassie for the labeling of proteins and protein complexes under native conditions...
  30. ncbi A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa
    G Q Zhang
    State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China
    Acta Biochim Pol 56:415-21. 2009
    ..In the case of the lectin from P. aurivella, its antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities have not been determined...
  31. doi Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions
    Daisuke Miyoshi
    Frontier Institute for Biomolecular Engineering Research, Konan University, 8 9 1 Okamoto, Higashinada ku, Kobe 658 8501, Japan
    J Am Chem Soc 131:3522-31. 2009
    ..2 +/- 0.2 kcal mol(-1), respectively. Furthermore, it was revealed that the opposing effects of molecular crowding on the Hoogsteen and Watson-Crick base pairs were due to different behaviors of water molecules binding to the DNA strands...
  32. ncbi Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism
    Nicolas Rouhier
    Unité Mixte de Recherche IaM INRA UHP Nancy I, Biochimie et Biologie Moléculaire Végétales, Universite Henri Poincare, 54506 Vandoeuvre Cedex, France
    J Biol Chem 277:13609-14. 2002
    ..Based on these experimental results, a catalytic mechanism is proposed to explain the Grx- and Trx-dependent activities of poplar Prx...
  33. pmc Delivery of the non-membrane-permeative antibiotic gentamicin into mammalian cells by using Shigella flexneri membrane vesicles
    J L Kadurugamuwa
    Canadian Bacterial Diseases Network, Department of Microbiology, College of Biological Science, University of Guelph, Ontario, Canada
    Antimicrob Agents Chemother 42:1476-83. 1998
    ..Being bilayered, with outer faces composed of LPS and Ipa proteins, these MVs were readily engulfed by the otherwise impermeable membranes and eventually liberated their contents into the cytoplasmic substance of the host tissue...
  34. pmc Mapping of the p53 and mdm-2 interaction domains
    J Chen
    Department of Molecular Biology, Princeton University, New Jersey 08544 1014
    Mol Cell Biol 13:4107-14. 1993
    ..This region contains the transactivation domain of p53, suggesting that mdm-2 may inhibit p53 function by disrupting its interaction with the general transcription machinery...
  35. doi Effect of beta-cyclodextrin on the renaturation of enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    Etsushi Yamamoto
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Anal Biochem 381:273-5. 2008
    ..incubation time (several hours) on renaturation of enzymatic activity after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)...
  36. ncbi Purification of a novel low-molecular-mass laccase with HIV-1 reverse transcriptase inhibitory activity from the mushroom Tricholoma giganteum
    H X Wang
    Department of Microbiology, China Agricultural University, and State Key Laboratory of Agrobiotechnology, Beijing, China
    Biochem Biophys Res Commun 315:450-4. 2004
    ..The laccase was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-cellulose. It inhibited HIV-1 reverse transcriptase with an IC(50) of 2.2 microM...
  37. ncbi Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes
    Mahel Zeghouf
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6
    J Proteome Res 3:463-8. 2004
    ..We also present an adaptation of the SPA system for the efficient purification and identification of protein complexes in E. coli and, potentially, other bacteria...
  38. doi Reliable identification of clinically prevalent species and subspecies of staphylococci by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis
    Olinda Cabral da Silva Santos
    Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    Diagn Microbiol Infect Dis 64:1-5. 2009
    We identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 10 species and 5 subspecies of Staphylococcus among 139 clinical isolates and compared it with conventional tests...
  39. pmc Resolving mitochondrial protein complexes using nongradient blue native polyacrylamide gel electrophoresis
    Liang Jun Yan
    Department of Pharmacology and Neuroscience and Institute for Aging and Alzheimer s Disease Research, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX 76107, USA
    Anal Biochem 389:143-9. 2009
    Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful technique for separation and proteomics analysis of high-molecular-weight protein complexes...
  40. ncbi Electrophoretic mobility of Alzheimer's amyloid-beta peptides in urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    John K Kawooya
    Lead Discovery Technologies Protein Production, JR1 2201, Aventis Pharmaceuticals, 1041 Route 202 206 North, Bridgewater, NJ 08807 0800, USA
    Anal Biochem 323:103-13. 2003
    ..This conformer has a high tendency to aggregate during conventional SDS-polyacrylamide gel electrophoresis (PAGE)...
  41. ncbi Polyacrylamide gel electrophoresis of fluorophore-labeled hyaluronan and chondroitin sulfate disaccharides: application to the analysis in cells and tissues
    Evgenia G Karousou
    Department of Experimental and Clinical Biomedical Sciences, University of Insubria, Varese, Italy
    Electrophoresis 25:2919-25. 2004
    This report describes a new formulation of polyacrylamide gel electrophoresis of fluorophore-labeled saccharides (PAGEFS) for the analysis of hyaluronan (HA) and chondroitin sulfate (CS) Delta-disaccharides...
  42. ncbi Cardiolipin stabilizes respiratory chain supercomplexes
    Kathy Pfeiffer
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    J Biol Chem 278:52873-80. 2003
    ..We show that a cardiolipin-deficient strain harbored almost inactive resting cytochrome c oxidase in the membrane. Transition to the fully active pulsed state occurred on a minute time scale...
  43. ncbi Investigating the fate of activated sludge extracellular proteins in sludge digestion using sodium dodecyl sulfate polyacrylamide gel electrophoresis
    Chul Park
    Department of Civil and Environmental Engineering, University of Massachusetts, Amherst, MA 01003, USA
    Water Environ Res 80:2219-27. 2008
    ..investigated using three different cation-associated extraction methods and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
  44. ncbi Fragmentation pattern of mucins in normal and diseased gastric mucosae: a glycoprotein fractionates with gastric mucins purified from mucosal scrapings of cancer and peptic ulcer patients
    A S Mall
    Department of Surgery and MRC Liver Research Centre, University of Cape Town Medical School, Cape Town, South Africa
    Digestion 60:216-26. 1999
    ..The aim of this study was a biochemical characterisation of gastric mucins in this disease, compared with gastric ulceration and controls from transplant donors...
  45. ncbi Isolation of cucurmoschin, a novel antifungal peptide abundant in arginine, glutamate and glycine residues from black pumpkin seeds
    H X Wang
    Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China
    Peptides 24:969-72. 2003
    A novel antifungal peptide, with a molecular mass of 8 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in gel filtration on Superdex 75 and designated cucurmoschin, was isolated from the seeds of the black pumpkin...
  46. pmc Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc
    K A Montzka
    Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven 06510
    Proc Natl Acad Sci U S A 85:8885-9. 1988
    ..Potential roles for the low-abundance snRNPs in aspects of pre-mRNA processing are discussed...
  47. ncbi Characteristics of human prostasomes isolated from three different sources
    Lena Carlsson
    Department of Medical Sciences, Clinical Chemistry Unit, University Hospital, Uppsala, Sweden
    Prostate 54:322-30. 2003
    ..Prostasomes are organelles secreted by human prostate epithelial cells and appear freely in semen. Most prostasomal functional and biochemical characteristics have been settled on basis of seminal prostasomes (SP)...
  48. ncbi In situ alkylation with acrylamide for identification of cysteinyl residues in proteins during one- and two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis
    Reiko Mineki
    Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, Tokyo, Japan
    Proteomics 2:1672-81. 2002
    Cysteinyl residues in proteins were alkylated with acrylamide during sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) to yield a thioether derivative, cys-S-beta-propionamide (PAM cys)...
  49. ncbi Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and forster resonance energy transfer suggest weak interactions between fibroblast growth factor receptor 3 (FGFR3) transmembrane domains in the absence of extracellular domains and ligands
    Edwin Li
    Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 44:352-60. 2005
    ..We have performed sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with peptides produced via solid-phase peptide synthesis that correspond to the TM domain ..
  50. pmc High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis
    Tatiana N Laremore
    Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Anal Biochem 401:236-41. 2010
    ..ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment...
  51. ncbi Use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for routine identification of enzymatically digested proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    K L Stone
    HHMI Biopolymer Laboratory and W M Keck Foundation, Yale University, New Haven, CT 06519, USA
    Electrophoresis 19:1046-52. 1998
    ..LC-ESI-MS/MS) analysis of >100 tryptic digests carried out on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separated, Coomassie Blue-stained proteins that were prepared by >50 different ..
  52. ncbi Polyacrylamide gel electrophoresis followed by sodium dodecyl sulfate gradient polyacrylamide gel electrophoresis for the study of the dimer to monomer transition of human transthyretin
    Klaus Altland
    Institut fur Humangenetik, Justus Liebig Universitat, Giessen, Germany
    Electrophoresis 24:2265-71. 2003
    ..We have developed a simple double one-dimensional (D1-D) electrophoretic procedure with polyacrylamide gel electrophoresis (PAGE) followed by sodium dodecylsulfate (SDS) gradient PAGE to study the dimer to monomer ..
  53. doi Coupling of native liquid phase isoelectrofocusing and blue native polyacrylamide gel electrophoresis: a potent tool for native membrane multiprotein complex separation
    Gian Maria D'Amici
    Department of Environmental Sciences, Tuscia University, Viterbo, Italy
    J Proteome Res 7:1326-40. 2008
    ..isoelectrofocusing (N-LP-IEF) of protein complexes in the first dimension, followed by blue native polyacrylamide gel electrophoresis (BN-PAGE) in the second dimension, where both the pI and the molecular masses of protein complexes (..
  54. ncbi A multidimensional differential proteomic platform using dual-phase ion-exchange chromatography-polyacrylamide gel electrophoresis/reversed-phase liquid chromatography tandem mass spectrometry
    Andrew K Ottens
    Center for Neuroproteomics and Biomarker Research, University of Florida, Gainesville, Florida 32610, USA
    Anal Chem 77:4836-45. 2005
    ..platform, collectively termed CAX-PAGE/RPLC-MSMS, combines biphasic ion-exchange chromatography with polyacrylamide gel electrophoresis for protein separation, quantification, and differential band targeting, followed by capillary ..
  55. pmc Use of DNA ladders for reproducible protein fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantitative proteomics
    Guoan Zhang
    Department of Pharmacology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York City, New York 10016, USA
    J Proteome Res 7:678-86. 2008
    In proteomics, one-dimensional (1D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein fractionation prior to mass spectrometric analysis to enhance the dynamic range of analysis and to improve ..
  56. ncbi A novel WD repeat protein component of the methylosome binds Sm proteins
    Westley J Friesen
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    J Biol Chem 277:8243-7. 2002
    ....
  57. ncbi Rapid protein separations in ultra-short microchannels: microchip sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing
    Jongyoon Han
    Sandia National Laboratories, Livermore, CA, USA
    J Chromatogr A 1049:205-9. 2004
    We have developed novel protein gel electrophoresis techniques, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) in short microchannels (approximately millimeters) that take less than a ..
  58. ncbi Identification of some rabbit allergens as lipocalins
    J Baker
    Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia
    Clin Exp Allergy 31:303-12. 2001
    ..The 18 kDa allergen from saliva may be the previously named rabbit allergen, Ory c 1...
  59. ncbi YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli
    Juliana C Malinverni
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Microbiol 61:151-64. 2006
    ..Genetic analysis verifies the importance of YfiO and its interactions with NlpB in maintaining the functional integrity of the YaeT complex...
  60. pmc Supercomplexes in the respiratory chains of yeast and mammalian mitochondria
    H Schagger
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    EMBO J 19:1777-83. 2000
    ..A fraction of total bovine ATP synthase (complex V) was isolated in dimeric form, suggesting that a dimeric state is not limited to S.cerevisiae, but also exists in mammalian mitochondria...
  61. ncbi Partial characterization of bacteriocins produced by human Lactococcus lactis and Pediococccus acidilactici isolates
    M Millette
    INRS Institut Armand Frappier, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre, 531, boulevard des Prairies, Laval, Quebec, Canada, H7V 1B7
    J Appl Microbiol 102:274-82. 2007
    ..The aim of this study was to isolate bacteriocin-producing lactic acid bacteria (LAB) from human intestine...
  62. ncbi Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov
    P Vandamme
    Laboratorium voor Microbiologie, Gent, Belgium
    Int J Syst Bacteriol 49:663-9. 1999
    ..Strains have been isolated from various human clinical and environmental sources...
  63. ncbi Role of the Ada2 and Ada3 transcriptional coactivators in histone acetylation
    Ramakrishnan Balasubramanian
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 1014, USA
    J Biol Chem 277:7989-95. 2002
    ..Our results suggest that Ada2 potentiates the Gcn5 catalytic activity and that Ada3 facilitates nucleosomal acetylation and an expanded lysine specificity...
  64. ncbi Analytical method for detection of beta-amylase isozymes in dehydrated cucumber cotyledons by using two-dimensional polyacrylamide gel electrophoresis
    Daisuke Todaka
    Department of Plant Production, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183 8509, Japan
    Anal Biochem 365:277-9. 2007
  65. ncbi A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus
    Y R Li
    State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China
    Biochim Biophys Acta 1780:51-7. 2008
    ..The lectin inhibited HIV-1 reverse transcriptase with an IC50 of 0.93 microM. It was devoid of antifungal activity...
  66. doi Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study
    Nicola A Burgess-Brown
    The Structural Genomics Consortium, Old Road Campus Research Building, University of Oxford, Roosevelt Drive, Oxford OX3 7DQ, UK
    Protein Expr Purif 59:94-102. 2008
    ..The trend is that heterologous expression of some proteins in bacteria can be improved by altering codon preference, but that this effect can be generally recapitulated by introducing rare codon tRNAs into the host cell...
  67. ncbi Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov
    T Coenye
    Laboratorium voor Microbiologie, Universiteit Gent, Belgium
    Int J Syst Bacteriol 49:405-13. 1999
    ..The six environmental and four human isolates belonged to the genus Ralstonia and were assigned to a new species for which the name Ralstonia gilardii sp. nov. is proposed. The type strain is LMG 5886T...
  68. ncbi Critical residues for structure and catalysis in short-chain dehydrogenases/reductases
    Charlotta Filling
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 277:25677-84. 2002
    ..This extends the previously recognized catalytic triad of Ser-Tyr-Lys residues to form a tetrad of Asn-Ser-Tyr-Lys in the majority of characterized short-chain dehydrogenases/reductase enzymes...
  69. ncbi Purification and characterization of a multiprotein component of the Drosophila 26 S (1500 kDa) proteolytic complex
    A Udvardy
    Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged
    J Biol Chem 268:9055-62. 1993
    ..In chicken, rabbit and human cells a high molecular weight multiprotein complex can be detected, which is immunologically related to the Drosophila mu particle...
  70. ncbi A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase
    S Triebel
    Department of Biochemistry, Faculty of Chemistry, University of Bielefeld, Germany
    FEBS Lett 314:386-8. 1992
    ..The amino acid sequence of tryptic peptides of this protein revealed homology with an alpha 2-microglobulin-related protein from rats, a protein so far unknown in humans...
  71. ncbi Characterization of renal damage in canine leptospirosis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting of the urinary proteins
    C Zaragoza
    Departamento de Medicina y Sanidad Animal, Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, Avda Universidad s n, Cáceres 10004, Spain
    J Comp Pathol 129:169-78. 2003
    ..To determine the urine protein pattern in the disease we performed sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on the urine from 10 dogs with leptospirosis and 20 healthy dogs...
  72. pmc Structural basis for the inhibition of the essential Plasmodium falciparum M1 neutral aminopeptidase
    Sheena McGowan
    Department of Biochemistry and Molecular Biology and Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria, 3800, Australia
    Proc Natl Acad Sci U S A 106:2537-42. 2009
    ..These results set the groundwork for the development of antimalarial therapeutics that target the neutral aminopeptidases of the parasite...
  73. ncbi Analysis of mitochondrial subunit assembly into respiratory chain complexes using Blue Native polyacrylamide gel electrophoresis
    Matthew McKenzie
    Department of Biochemistry, La Trobe University, Melbourne, VIC 3086, Australia
    Anal Biochem 364:128-37. 2007
    ..complexes using radiolabeled, mitochondrially encoded subunits in conjunction with Blue Native polyacrylamide gel electrophoresis. The efficiency of assembly was found to differ markedly between complexes, and intermediate ..
  74. ncbi Large-format, two-dimensional polyacrylamide gel electrophoresis of ovine periimplantation uterine luminal fluid proteins: identification of aldose reductase, cytoplasmic actin, and transferrin as conceptus-synthesized proteins
    R S Lee
    Molecular Embryology AgResearch, Ruakura Research Center, Hamilton, New
    Biol Reprod 59:743-52. 1998
    ....
  75. ncbi A novel Bicine running buffer system for doubled sodium dodecyl sulfate - polyacrylamide gel electrophoresis of membrane proteins
    Taufika Islam Williams
    Department of Chemistry, University of Kentucky, Lexington, KY 40506 0286, USA
    Electrophoresis 27:2984-95. 2006
    ..The data clearly indicated that Bicine-dSDS-PAGE is a superior method for the analysis of membrane proteins, providing enhanced resolution and protein representation...
  76. ncbi Energetics and cooperativity of tertiary hydrogen bonds in RNA structure
    S K Silverman
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado at Boulder 80309 0215, USA
    Biochemistry 38:8691-702. 1999
    ..4 to -0.5 kcal/mol per tertiary hydrogen bond may be more generally applicable to RNA folding...
  77. ncbi Monoclonal antibody-mediated tumor regression by induction of apoptosis
    B C Trauth
    Institute for Immunology and Genetics, German Cancer Research Center, Heidelberg
    Science 245:301-5. 1989
    ..Thus, induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO-1 may be a useful therapeutic approach in treatment of malignancy...
  78. ncbi N- and C-Terminal motifs in human alphaB crystallin play an important role in the recognition, selection, and solubilization of substrates
    Joy G Ghosh
    Biomolecular Structure and Design, Department of Biological Structure, University of Washington, Seattle, Washington 98195 7420, USA
    Biochemistry 45:13847-54. 2006
    ..The results presented in this report established that interactive domains in the N- and C-termini of human alphaB crystallin are important for the recognition, selection, and solubility of unfolding substrate proteins...
  79. doi The three domains of the mitochondrial outer membrane protein Mim1 have discrete functions in assembly of the TOM complex
    Franziska Lueder
    Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Australia
    FEBS Lett 583:1475-80. 2009
    ..In addition, a "late reaction" catalysed by the Sam37 subunit of the SAM complex is also influenced by Mim1. Thus, Mim1 participates at multiple stages in the assembly of the TOM complex...
  80. doi A unique caleosin in oil bodies of lily pollen
    Pei Luen Jiang
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan
    Plant Cell Physiol 49:1390-5. 2008
    ..Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies...
  81. ncbi Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis
    Emiko Kinoshita-Kikuta
    Department of Functional Molecular Science, Graduate School of Biomedical Sciences, Kasumi 1 2 3, Hiroshima University, Hiroshima 734 8553, Japan
    Mol Cell Proteomics 6:356-66. 2007
    ..describe three applications of label-free kinase profiling using a novel type of phosphate affinity polyacrylamide gel electrophoresis. The phosphate affinity site is a polyacrylamide-bound dinuclear Mn2+ complex that enables the ..
  82. pmc Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis
    Jerri Ross
    Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8026, USA
    J Clin Virol 42:374-80. 2008
    ....
  83. doi Sequence effects in single-base loops for quadruplexes
    Aurore Guédin
    INSERM, U565, Acides nucléiques dynamique, ciblage et fonctions biologiques, 43 rue Cuvier, CP26, F 75231 Paris Cedex 05, France
    Biochimie 90:686-96. 2008
    ....
  84. doi A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis
    Chun Yi Liau
    Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, R O C Department of Bioengineering, Tatung University, Taipei 104, Taiwan, ROC
    J Biosci Bioeng 106:111-3. 2008
    ..Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme...
  85. pmc HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim
    Dan Chen
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3206, USA
    EMBO J 21:6801-10. 2002
    ..Our findings reveal a strategy by which Tat induces apoptosis by targeting the microtubule network. Thus HIV-1 Tat joins a growing list of pathogen-derived proteins that target the cytoskeleton of host cells...
  86. doi Expression, reconstitution, and structure of an archaeal RNA degrading exosome
    Esben Lorentzen
    Birkbeck College London, Institute of Structural Molecular Biology, London, United Kingdom
    Methods Enzymol 447:417-35. 2008
    ..Furthermore, we describe several crystal structures in which RNA substrates were diffused into crystals and how anomalous scattering from 5-iodo-uridine-modified RNA was used to locate low-occupancy RNA binding sites...
  87. doi Changes in ferritin H- and L-chains in canine lenses with age-related nuclear cataract
    Malgorzata Goralska
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, North Carolina 27606, USA
    Invest Ophthalmol Vis Sci 50:305-10. 2009
    ..To determine potential differences in the characteristics of the iron storage protein ferritin and its heavy (H) and light (L) subunits in fiber cells from cataractous and noncataractous lenses of older dogs...
  88. ncbi Potential pathogenic role of aggregative-adhering Corynebacterium diphtheriae of different clonal groups in endocarditis
    R Hirata Jr
    Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciencias Medicas, Universidade do Estado do Rio de Janeiro
    Braz J Med Biol Res 41:986-91. 2008
    ..diphtheriae of different clones in endocarditis. Accordingly, the aggregative-adherence pattern may be used as an indication of some invasive potential of C. diphtheriae strains...
  89. ncbi Gender difference in the composition of fecal flora in laboratory mice, as detected by denaturing gradient gel electrophoresis (DGGE)
    Seigo Fushuku
    Center for Laboratory Animal Science, National Defense Medical College, Saitama, Japan
    Exp Anim 57:489-93. 2008
    ..Male and female mice were distributed on opposite sides of the origin of the plane in NMDS at weeks 0, 2, 3, and 8. These results suggest a gender difference in the composition of intestinal flora in mice...
  90. doi Electrophoresis techniques to investigate defects in oxidative phosphorylation
    Maria Antonietta Calvaruso
    Nijmegen Center for Mitochondrial Disorders at the Department of Pediatrics, Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Methods 46:281-7. 2008
    ..Here we provide a practical guide on how to set-up the basic technique to study OXPHOS defects in patient-derived cells and tissues...
  91. doi Proteomics analysis of nasopharyngeal carcinoma cell secretome using a hollow fiber culture system and mass spectrometry
    Hsin Yi Wu
    Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    J Proteome Res 8:380-9. 2009
    ..We suggest that the HFC system may be used to collect secreted proteins efficiently and facilitate comprehensive characterization of cell secretome...
  92. pmc Proteasomal protein degradation in Mycobacteria is dependent upon a prokaryotic ubiquitin-like protein
    Kristin E Burns
    Tuberculosis Research Section, NIAID, NIH, Bethesda, MD 20892, USA
    J Biol Chem 284:3069-75. 2009
    ..These data therefore establish that, despite differences in both sequence and target linkage, Pup plays an analogous role to ubiquitin in targeting proteins to the proteasome for degradation...
  93. doi Preparation of genome-wide DNA fragment libraries using bisulfite in polyacrylamide gel electrophoresis slices with formamide denaturation and quality control for massively parallel sequencing by oligonucleotide ligation and detection
    Swati S Ranade
    Applied Biosystems, Foster City, CA 94404, USA
    Anal Biochem 390:126-35. 2009
    ....
  94. pmc Inositol pyrophosphates and their unique metabolic complexity: analysis by gel electrophoresis
    Oriana Losito
    Department of Cell and Developmental Biology, Medical Research Council Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London, London, United States of America
    PLoS ONE 4:e5580. 2009
    ..Though likely involved in a wide range of cellular functions, the study of inositol pyrophosphates has suffered from a lack of readily available methods for their analysis...
  95. pmc The beta-barrel outer membrane protein assembly complex of Neisseria meningitidis
    Elena B Volokhina
    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Bacteriol 191:7074-85. 2009
    ..Thus, the Bam complex in N. meningitidis consists of Omp85/BamA plus RmpM, BamC, ComL/BamD, and BamE, of which ComL/BamD and BamE appear to be the most important accessory components for OMP assembly...
  96. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
    ..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors...
  97. doi Roles of a membrane-bound caleosin and putative peroxygenase in biotic and abiotic stress responses in Arabidopsis
    Mark Partridge
    Biotechnology Unit, Division of Biological Sciences, University of Glamorgan, Treforest, CF37 1DL, United Kingdom
    Plant Physiol Biochem 47:796-806. 2009
    ..We propose that Clo-3 is part of an oxylipin pathway induced by multiple stresses and may also generate fatty acid derived anti-fungal compounds for plant defence...
  98. pmc Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease
    Ann Saada
    Metabolic Disease Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    Am J Hum Genet 84:718-27. 2009
    ..These data not only show that NDUFAF3 mutations cause complex I deficiency but also relate different complex I disease genes by the close cooperation of their encoded proteins during the assembly process...
  99. doi Proteomic analysis of membrane microdomain-associated proteins in the dorsolateral prefrontal cortex in schizophrenia and bipolar disorder reveals alterations in LAMP, STXBP1 and BASP1 protein expression
    A T Behan
    Department of Psychiatry, Royal College of Surgeons in Ireland, RCSI ERC, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland
    Mol Psychiatry 14:601-13. 2009
    ..methods, two-dimensional difference gel electrophoresis and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by reverse phase-liquid chromatography-tandem mass spectrometry (RP-LC-MS/MS) (gel ..
  100. pmc Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells
    Lisa Ott
    Friedrich Alexander Universitat Erlangen Nurnberg, Lehrstuhl fur Mikrobiologie, Staudtstr 5, 91058 Erlangen, Germany
    BMC Microbiol 10:2. 2010
    ..diphtheriae factors crucial for colonization of the host. In this study, we investigated the function of surface-associated protein DIP1281, previously annotated as hypothetical invasion-associated protein...
  101. pmc Histone H4 N-terminal acetylation in Kasumi-1 cells treated with depsipeptide determined by acetic acid-urea polyacrylamide gel electrophoresis, amino acid coded mass tagging, and mass spectrometry
    Liwen Zhang
    Mass Spectrometry and Proteomics Facility, Campus Chemical Instrument Center, Department of Chemistry, Division of Hematology Oncology, The Ohio State University, Columbus, Ohio 43210, USA
    J Proteome Res 6:81-8. 2007
    ..We describe here an analytical approach that combines acid urea polyacrylamide gel electrophoresis (AU-PAGE), amino acid coded mass tagging (AACM), and mass spectrometry (MS) for the investigation of ..

Research Grants72

  1. BIOPHYSICS AND GENETICS OF VIRAL DNA PACKAGING
    Philip Serwer; Fiscal Year: 1992
    ..binding of fluorescent structure probes, protein-protein crosslinking, sodium dodecyl sulfate polyacrylamide gel electrophoresis and protease digestion will be used; (d) isolate new DNA packaging intermediates; packaging will be ..
  2. MOLECULAR STRUCTURE/FUNCTION OF TEKTINS IN MICROTUBULES
    RICHARD LINCK; Fiscal Year: 1993
    ..Finally, using the cDNA probes, the expression and role of tektins in sea urchin embryonic ciliogenesis will be investigated...
  3. EFFECT OF MARIJUANA ON MACROPHAGE ANTIGEN PRESENTATION
    Guy Cabral; Fiscal Year: 1993
    ..14C]-amino acids, or [3H]-glucosamine, and subjected to one-dimensional or two-dimensional isodalt polyacrylamide gel electrophoresis in concert within radioautography, will be performed to establish the effect of THC on the time and ..
  4. NUCLEAR PROTEIN CONTENT AND HEAT-INDUCED CELL KILLING
    Joseph Roti Roti; Fiscal Year: 1993
    ..This aim will be accomplished by analyzing nuclear proteins by polyacrylamide gel electrophoresis (PAGE) immediately after various heat exposures. 3...