biological dressings

..We therefore suggest that immunogenicity of AE should not be ignored, and use of AM from different donor placentas should be emphasized when repeated AM transplantation is required in patients clinically...

..Advanced healing therapies employing biological dressings, skin substitutes, growth factor-based therapies and synthetic acellular matrices, all of which aim to ..

Tissue-engineered biological dressings offer promise in the treatment of burns, chronic ulcers, donor site and other surgical wounds, and a variety of blistering and desquamating dermatologic conditions...

..However, further work is needed to elucidate whether it functions merely as a biologic contact lens or whether it has additional benefits...

..To evaluate how sutureless amniotic membrane patches may affect visual functions in normal human eyes...

..To assess the efficacy of amniotic membrane for treatment of partial limbal stem cell deficiency (LSCD)...

..This option is easy-to-use and enables psychoemotional stress associated with a surgical intervention to be avoided. It may be employed in patients with severe somatic diseases, its clinical effectiveness being retained...

..To investigate the expression of laminin-5 during epithelial healing and evaluate its expression in vivo using rat corneas on which amniotic membrane was applied to cover the wound after excimer laser photoablation...

..The study has demonstrated the ability of silver-impregnated amniotic membrane to combat microbial infection and its ideal physical characteristics for clinical use as a burn wound dressing...

..To determine if multilayer amniotic membrane transplantation (AMT) is useful in the treatment of corneal perforations, and in particular to assess to what extent efficacy is affected by perforation size...

..Ulcers with duration of minimum 3 weeks to maximum 12 months were found to heal in 2-6 weeks by the application of amniotic membranes...

..This article provides an overview of the currently available products which have already been introduced into clinical routine as well as describing advantages and disadvantages of the individual products and their indications...

..Therefore, the classic concept of topical therapy will be expanded and developed within two major stages: acute and rehabilitation. Comparison of the effectiveness of the different treatments and relevant literature will be discussed...

..It decreases: 1) the possibility of infection, and therefore reduces the consumption of antibiotic; 2) the use of analgesics; 3) the time of scaring and 4) the number of days of hospitalization...

..001). CONCLUSION: Amniotic membrane use after LASEK induced rapid epithelial healing with more favorable visual and refractive outcomes and lower corneal haze scores than conventional LASEK...

..Our study showed that fresh or acellular bovine AMs provided similar efficacy for wound healing as did the fresh human AMs...

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..It is expected that cultured epithelial cell grafts will prove to be an effective treatment not only for extensive burns but also to epithelialize small area burn wounds and resurfaced burn scars...

..At least 3 months may be needed for the proliferation and differentiation of in vivo cultivated corneal epithelium on AM...

..The application of radiation sterilized amniotic membranes on the burn wound favoured epithelialization. In all the patients, membranes dessicated and separated in 10-14 days time leaving behind an epithelialized surface...

..Following a debridement conservative treatment of superficial dermal burns involving wound coverage with biosynthetic dressings or nanocristalline silver gauze dressings or use of special disinfecting ointments can be implemented...

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..Thus, this study could provide basic data on the clinical application of the AMO in the days ahead...

..In addition, the beneficial effects of reducing inflammation and neovascularization persist for a long time, if properly cryopreserved...

..To establish the effectiveness of amniotic membrane transplantation (AMT) in relieving pain and discomfort in patients with painful bullous keratopathy and its role in improving vision in eyes with visual potential...

..Since accurate information about alternative cures is scanty, this study was conducted to assess the outcome of the use of xenoderm biologic dressings as one alternative...

..AM transplantation may thus be considered to be an alternative method for treating chronic leg ulcers...

..The removal of amniotic epithelial cells using our protocol does not appear to have any significant effects on the structure of the basal lamina or the distribution of ECM components...

..CONCLUSIONS: Both amniotic membrane transplantation and conventional management (tarsorrhaphy or bandage contact lens) are effective treatment modalities for refractory neurotrophic corneal ulcers...

..To study the association between surgical approach and postoperative results in chemical and thermal burns of the cornea...

..Further studies will undoubtedly reveal the true potential of the membrane, its mechanism(s) of action, and the effective use of this tissue in ophthalmology...

..The authors report complications of corneal surface disorders after successful amniotic membrane transplantation...

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..AMT is effective in managing large OSN by avoiding the complications that may be associated with conventional removal, specifically in cases where the limbal architecture is destroyed by surgical resection or adjuvant therapies...

..Thus, CCA should be considered as an initial treatment of choice for removal of eschar in children, having a partial-thickness burn wound without infection...

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..There was no evidence of recurrent epithelial ingrowth after a minimum of 4 months. Use of an amniotic membrane overlay can be an effective adjuvant therapy to secure a LASIK flap when direct suturing would threaten the visual axis...

..To describe amniotic membrane transplantation indications and results at the authors' institution...

..To evaluate the clinical outcome of a new sutureless approach for a temporary amniotic membrane patch (ProKera; Bio-Tissue, Inc, Miami, Florida) in eyes with acute burns...

..We postulate that angiogenic effects of this biological dressing may have contributed to the improved healing conditions and successful secondary wound closure...

..OrCel sites also exhibited reduced scarring. Therefore, treatment of donor site wounds with OrCel is well tolerated, promotes more rapid healing, and results in reduced scarring when compared with conventional therapy with Biobrane-L...

..5 months, and 5 months after surgery demonstrated closure of the conjunctival defect. The author concludes that amniotic membrane may be useful for the treatment of hydroxyapatite orbital implant exposures...

..This article is a chronological review of the important steps that brought ex vivo expanded stem cell transplantation in ocular, particularly corneal surface reconstruction...

..This paper shows the excellent results obtained with the use of this extremely economical dressing and emphasizes the importance of establishing such "Amnion Banks" in all hospitals especially in developing countries...

..The procurement, complications, and solutions of this delicate procedure are covered with a mention of what is in store for the future...

..The early detachment of the amniotic membrane implant remains a major problem, even with the use of multiple fixation sutures...

..To report the results of the management of painfully symptomatic postoperative bullous keratopathy (PBK) by performing a nonpreserved human amniotic membrane (NP-AMT) transplantation in nine eyes with poor visual potential...

..Anyway the 3-dimensional model proposed seems to be suitable for the study of the behaviour of peripheral CD 34+ and perhaps also other types of stem cells in 3-dimensional dermal matrix...

Biological products (tissue engineered skin, allograft and xenograft, and biological dressings) are widely used in the treatment of burns, chronic wounds, and other forms of acute injury...

..Numerous treatment protocols with variable energy settings allow for individualized treatments and provide the operator with fine control over the degree of injury and length of subsequent recovery time...

..This technique has been employed in patients where standard tarso-conjunctival flaps are not an option and free posterior lamellar grafts cannot be supported due to the lack of viable anterior lamellar flaps...

..This is in concordance with other reports on scalds. It would be worthwhile to perform more clinical studies with a larger number of patients to further evaluate the effect and function of allogenic skin for facial burns...

..Allogeneic CDS functions as an excellent biological dressing, and could dramatically change the treatment of intractable skin ulcers...

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..They do not require a biopsy from the patient, an advantage compared to autografts. They seem to act as a biological dressing, stimulating epithelialization from the wound edges and adnexae, probably through growth factor release...

..The possible mechanisms of the stimulatory effect of collagen-based dressing on wound healing are discussed...

..both directly during surgical debridement and indirectly by reducing infection and optimising the use of biological dressings. In deep partial thickness burns softer necrotic dermis can be removed with the Versajet sparing the ..

Tissue-engineered biological dressings offer promise in the treatment of burns, chronic ulcers, donor site and other surgical wounds, and a variety of dermatologic conditions...

..We also performed a flow cytometric analysis of sorted cells using a cell tracer...

..Adhering to a proposed weekly working schedule makes the rearing procedure effective and reliable...

..It has been demonstrated that mesenchymal stem cells are present in the adipose tissue (thus accelerating the effect of the PRP)...

..The possibilities of using epidermal stem cells in medicine are also presented...

..This article reviews the published data on microdebrider-assisted partial intracapsular tonsillectomy with a discussion of its advantages, potential limitations and areas of future research...

..The observed advantages of human amnion treatment include pain relief, ease of use, prevention of infection and acceleration of wound healing...

..To evaluate tonsillar wound healing histopathology in a canine model following microdebrider intracapsular and electrocautery tonsillectomy techniques...

..Lyophilized bovine collagen matrix is one of ideal biological dressings used in wound treatment. We present an example of type 1 bovine collagen (Gelfix, Euroresearch, Inc...

..Our results demonstrate that CTLA4Ig gene-modified pig skin is a promising biologic dressing for wound coverage and Ad5F35 an effective viral carrier for delivery...

..environment for fibroblast adhesion, proliferation and biological activity and therefore constitute an original three-dimensional environment for in vitro cell culture and in vivo applications, in particular as biological dressings.

..Although the success of these therapies is enticing, their use should be based on careful patient selection in a multidisciplinary setting...

..with wet-to-dry dressings were followed by admission to several facilities and 15 months of care with biological dressings and negative pressure wound therapy (NPWT)...

..This article will review honey, human amnion, xenograft, allograft, cultured epithelial autograft, and various engineered commercial products for use in the biologic treatment of burn wounds...

..The wound healed without adhesion formation. CONCLUSION: Excision plus amniotic membrane grafting is effective for recurrent labial adhesions...

..The presence of several cytokines in the amniotic membrane promotes epithelialization with reduction of fibrosis during healing...

..Staphylococcus aureus and beta-haemolytic streptococci are the most common causative organisms of cutaneous infections...

..A review of available dressing materials is reported with emphasis on the newly developed concept of moist environment for optimal healing. a practical guide for dressing selection is also proposed...

..No adverse reactions were recorded.Hyalomatrix PA is used in young and adults, in deep partial thickness and full thickness burns, as a temporary coverage before grafting or alone for wound healing...

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..0096). Hair growth is homogenous in both groups on day 30 and day 60 (ns). These results showed that scalp is a very interesting skin donor site and that Algosteril is of a real interest in donor site treatment...

..Cultured epithelial autograft (CEA) sheets provide an alternative means of covering debrided skin. This is a report on an initial experience with using CEA sheets...

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..This review focuses on the rationale for the use of these emerging tools, how they are prepared, clinical applications, limitations, and speculates on future directions...

..A cost-effectiveness analysis compared the potential economic benefit of an autologous, platelet-rich plasma (PRP) gel to alternative therapies in treating nonhealing diabetic foot ulcers...

..0% of genes over-expressed in fetal fibroblasts. It is perhaps these differences which contribute to efficient tissue repair seen in the clinic with fetal cell therapy...

..The aim of the present study was to synthesise and characterise chitosan-gelatin antimicrobial copolymer...

..Severe forms of epidermolysis bullosa (EB) are characterized by chronic, nonhealing wounds...

..The interaction between the various components of the biologic resorbable matrix seams to participate direct into the conjonctive healing process and ensure an active protection of surgical sites...

..To describe the clinical features of patients affected with vernal keratoconjunctivitis (VKC) with associated limbal stem cell deficiency (LSCD)...

..On the basis of the wound healing results, patients' evaluation of treatment and the clinicians' and staff experiences, this concept was implemented at different Surgical Departments of the Charité-Universitätsmedizin in Berlin...

..To report the clinical results of patients treated by preserved human amniotic membrane transplantation (AMT) following the removal of conjunctival and limbal tumors...

..We report our experience in the treatment of pediatric TEN patients with early debridement of necrotic skin and coverage with human allograft skin...

..The purpose of the present study was to confirm the clinical expediency of using a particular type of biological skin substitute--porcine skin xenograft--in the treatment of partial-thickness scald burns...

..Reimbursement issues are also discussed...

..These materials can protect the wound, increase healing, provide overall wound coverage, and improve patient care. The ideal skin substitute may soon become a reality...

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..It is anticipated that findings in this animal model will provide a basis for investigation of wound macrophage phenotypes in human disease, and may identify targets for therapeutic interventions in abnormal healing. ..