indirect fluorescent antibody technique


Summary: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)

Top Publications

  1. doi Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells
    Rico Hiemann
    Department of Biology, Chemistry and Process Technology, Lausitz University of Applied Sciences, Senftenberg, Germany
    Autoimmun Rev 9:17-22. 2009
  2. pmc Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body
    Jiang Hong Yu
    Department of Medicine, Havard Medical School, the Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Charlestown, MA 02129, USA
    RNA 11:1795-802. 2005
  3. ncbi A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis
    Vanda A Lennon
    Department of Neurology, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Lancet 364:2106-12. 2004
  4. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
  5. ncbi Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts
    R Tournebize
    Cell Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 2:13-9. 2000
  6. doi Autoantibody detection using indirect immunofluorescence on HEp-2 cells
    Ulrich Sack
    Institute of Clinical Immunology and Transfusion Medicine, Medical Faculty of the University, Leipzig, Germany
    Ann N Y Acad Sci 1173:166-73. 2009
  7. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  8. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
  9. doi Aquaporin-4 antibodies in neuromyelitis optica and longitudinally extensive transverse myelitis
    Patrick Waters
    Neurosciences Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England
    Arch Neurol 65:913-9. 2008
  10. ncbi Risk factors for infection with malaria in a low endemic community in Bataan, the Philippines
    M A Lansang
    Research Institute for Tropical Medicine, Department of Health, Alabang, Muntinlupa, Metro Manila, Philippines
    Acta Trop 63:257-65. 1997

Research Grants

Detail Information

Publications228 found, 100 shown here

  1. doi Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells
    Rico Hiemann
    Department of Biology, Chemistry and Process Technology, Lausitz University of Applied Sciences, Senftenberg, Germany
    Autoimmun Rev 9:17-22. 2009
    ..More sophisticated pattern recognition algorithms and novel calibration systems will improve standardised quantifications of IIF image interpretation...
  2. pmc Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body
    Jiang Hong Yu
    Department of Medicine, Havard Medical School, the Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Charlestown, MA 02129, USA
    RNA 11:1795-802. 2005
    ..Taken together, the results of this study show that Ge-1 is a central component of P-bodies and suggest that Ge-1 may act prior to the 5(')-decapping step in mRNA degradation...
  3. ncbi A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis
    Vanda A Lennon
    Department of Neurology, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Lancet 364:2106-12. 2004
    ..We assessed the capacity of a putative marker for neuromyelitis optica (NMO-IgG) to distinguish neuromyelitis optica and related disorders from multiple sclerosis...
  4. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  5. ncbi Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts
    R Tournebize
    Cell Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 2:13-9. 2000
    ..These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1...
  6. doi Autoantibody detection using indirect immunofluorescence on HEp-2 cells
    Ulrich Sack
    Institute of Clinical Immunology and Transfusion Medicine, Medical Faculty of the University, Leipzig, Germany
    Ann N Y Acad Sci 1173:166-73. 2009
    ..The aim is to improve diagnostic tests and care of patients with autoimmune diseases as a central concern of the European Autoimmunity Standardization Initiative (EASI)...
  7. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  8. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
    ..The three phases of methylation reprogramming may have roles in imprinting, the control of gene expression, and the establishment of nuclear totipotency...
  9. doi Aquaporin-4 antibodies in neuromyelitis optica and longitudinally extensive transverse myelitis
    Patrick Waters
    Neurosciences Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England
    Arch Neurol 65:913-9. 2008
    ..There is increasing recognition of antibody-mediated immunotherapy-responsive neurologic diseases and a need for appropriate immunoassays...
  10. ncbi Risk factors for infection with malaria in a low endemic community in Bataan, the Philippines
    M A Lansang
    Research Institute for Tropical Medicine, Department of Health, Alabang, Muntinlupa, Metro Manila, Philippines
    Acta Trop 63:257-65. 1997
    ..16, 0.52). The data suggest that in low-endemic communities like Morong, Bataan, control efforts should be primarily directed to focal areas identified by serology, particularly among migrants and among male young adults...
  11. pmc Nucleolar localization of the Werner syndrome protein in human cells
    R A Marciniak
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 95:6887-92. 1998
    ..Understanding the function of WRN in these organisms of vastly differing lifespan may yield new insights into the mechanisms of lifespan determination...
  12. pmc Heterogeneity of a fluorescent tegument component in single pseudorabies virus virions and enveloped axonal assemblies
    T del Rio
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    J Virol 79:3903-19. 2005
    ..These results support an assembly process in which PRV capsids acquire a membrane in the cell body prior to axonal entry and subsequent transport...
  13. ncbi The development of a serum-free derived bioengineered conjunctival epithelial equivalent using an ultrathin poly(epsilon-caprolactone) membrane substrate
    Leonard P K Ang
    Singapore National Eye Center, Singapore
    Invest Ophthalmol Vis Sci 47:105-12. 2006
    ..To evaluate the use of an ultrathin poly(epsilon-caprolactone) (PCL) membrane as a substrate for the development of a serum-free-derived conjunctival epithelial equivalent...
  14. doi The clinical significance of antinucleolar antibodies
    S Khan
    Path Links Immunology, Scunthorpe General Hospital, Scunthorpe, UK
    J Clin Pathol 61:283-6. 2008
    ..The importance of antinucleolar antibodies seen by indirect immunofluorescence on HEp-2 cells, although associated with systemic sclerosis (SSc), in unselected patients is unknown...
  15. ncbi Outbreak of cutaneous leishmaniasis in northern Israel
    Raymond L Jacobson
    Department of Parasitology, The Kuvin Center for Study of Infectious and Tropical Diseases, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Infect Dis 188:1065-73. 2003
    ..tropica foci. P. arabicus is the main vector, and it transmits parasites that are different from other L. tropica isolates, with respect to antigenic, molecular, and biochemical parameters...
  16. pmc G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214
    J van Deursen
    Department of Genetics, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    EMBO J 15:5574-83. 1996
    ..Our data suggest that CAN is critical to cell cycle progression and required for both nuclear protein import and mRNA export...
  17. ncbi Detection of anti-SSA antibodies by indirect immunofluorescence
    Xavier Bossuyt
    Laboratory Medicine and Internal Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 50:2361-9. 2004
    ..We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA...
  18. ncbi Detection of specific antinuclear reactivities in patients with negative anti-nuclear antibody immunofluorescence screening tests
    Ilse E A Hoffman
    Department of Rheumatology, Ghent University Hospital, De Pintelaan 185, Ghent 9000, Belgium
    Clin Chem 48:2171-6. 2002
    ..However, because anti-ENA reactivities are found in samples with low fluorescence intensities, we determined anti-ENA antibodies in samples with negative IIF and thus calculated the sensitivity of IIF for specific ANAs...
  19. ncbi In situ identification of mycobacteria in Crohn's disease patient tissue using confocal scanning laser microscopy
    S A Naser
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando 32816, USA
    Mol Cell Probes 16:41-8. 2002
    ..This technique adds an additional approach to investigate MAP role in CD etiology especially when the culture approach is long and inconsistent...
  20. pmc A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
    Theophany Eystathioy
    Department of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    Mol Biol Cell 13:1338-51. 2002
    ..We propose that the GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene transcripts involved in cell growth and homeostasis...
  21. ncbi Functional genomics of gam56: characterisation of the role of a 56 kilodalton sexual stage antigen in oocyst wall formation in Eimeria maxima
    Sabina I Belli
    Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Westbourne Street, Gore Hill, NSW 2065, Australia
    Int J Parasitol 32:1727-37. 2002
    ..In addition, these findings provide evidence for the molecular mechanisms underlying oocyst wall formation in Eimeria and the role of gametocyte antigens in this process...
  22. ncbi Cadherin-6, a cell adhesion molecule specifically expressed in the proximal renal tubule and renal cell carcinoma
    R Paul
    James Buchanan Brady Urological Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Cancer Res 57:2741-8. 1997
    ..Overall, these findings demonstrate specific expression of cadherin-6 in the proximal renal tubules in normal human kidney and suggest that alterations of cadherin-6 expression are associated with progression of renal cell carcinoma...
  23. ncbi Transient receptor potential (TRPC) channels in human sperm: expression, cellular localization and involvement in the regulation of flagellar motility
    Laura E Castellano
    Department of Genetics of Development and Molecular Physiology, Institute of Biotechnology, UNAM, Cuernavaca, Mexico
    FEBS Lett 541:69-74. 2003
    ..Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility...
  24. ncbi Biochemical characterisation of the 56 and 82 kDa immunodominant gametocyte antigens from Eimeria maxima
    Sabina I Belli
    Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Gore Hill, Westbourne Street, Sydney, N S W 2065, Australia
    Int J Parasitol 32:805-16. 2002
    ..Characterisation of these novel proteins has provided further insights into the molecular mechanisms of gametocyte differentiation in E. maxima...
  25. ncbi Anti-aquaporin-4 antibody is involved in the pathogenesis of NMO: a study on antibody titre
    Toshiyuki Takahashi
    Department of Neurology, Tohoku University Graduate School of Medicine, 1 1 Seiryomachi, Aobaku, Sendai 980 8574, Japan
    Brain 130:1235-43. 2007
  26. ncbi [Emerging and reemerging rickettsiosis in an endemic area of Minas Gerais State, Brazil]
    Marcio A M Galvao
    Departamento de Nutrição Clínica e Social, Escola de Nutrição, Universidade Federal de Ouro Preto, Ouro Preto, MG, 35400 000, Brasil
    Cad Saude Publica 18:1593-7. 2002
    ..81%), respectively. The results in schoolchildren and the presence of canine seroreactivity to Ehrlichiaspecies that are potentially pathogenic to humans suggests the risk of transmission of other Rickettsiaein the study area...
  27. ncbi Nestin as a marker for proliferative endothelium in gliomas
    Ken ichi Sugawara
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Lab Invest 82:345-51. 2002
    ..As expected, nestin immunostained strongly in all four samples of hemangioblastomas. We suggest that nestin is not only a marker for neuroepithelial stem cells and glioma cells but also for tumor endothelial cells during rapid growth...
  28. ncbi Seroprevalence of human granulocytic ehrlichiosis in high-risk groups in Denmark
    S Skarphedinsson
    Department of Clinical Microbiology, Odense University Hospital, Denmark
    Scand J Infect Dis 33:206-10. 2001
    ..Our study indicates that HGE infection with or without concomitant or previous Borrelia burgdorferi infection is common in tick-exposed individuals > 20 y old in the county of Funen, Denmark...
  29. ncbi Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae
    V C Cordes
    Division of Cell Biology, German Cancer Research Center, Heidelberg, Germany
    Exp Cell Res 237:419-33. 1997
    ..These data demonstrate that AL can represent distinct sites within the cytoplasm of transient accumulation of nuclear proteins and that the AL pore complex shares functional binding properties with the NPC...
  30. ncbi The heterotrimeric motor protein kinesin-II localizes to the midpiece and flagellum of sea urchin and sand dollar sperm
    J H Henson
    Department of Biology, Dickinson College, Carlisle, Pennsylvania 17013, USA
    Cell Motil Cytoskeleton 38:29-37. 1997
    ..We hypothesize that kinesin-II in the sperm may play functional roles in intraflagellar transport and/or the formation of flagella during spermatogenesis...
  31. ncbi Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion
    Stuart D Blacksell
    Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand
    Clin Infect Dis 44:391-401. 2007
    ..We suggest that the diagnosis of scrub typhus using IFA should be based on a > or =4-fold increase in the titer in paired serum samples and should only be based on a single sample titer when there is an adequate local evidence base...
  32. ncbi A comprehensive analysis of the expression of crystallins in mouse retina
    Jinghua Xi
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Vis 9:410-9. 2003
    ..This prompted us to undertake a comprehensive investigation to delineate the baseline expression of crystallin genes in the adult mouse retina...
  33. pmc RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery
    Asa E Y Engqvist-Goldstein
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Mol Biol Cell 15:1666-79. 2004
    ..Our results provide evidence that Hip1R helps to make the interaction between actin and the endocytic machinery functional and transient...
  34. ncbi p38 mitogen-activated protein kinase (MAPK) first regulates filamentous actin at the 8-16-cell stage during preimplantation development
    Andrew J M Paliga
    Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1
    Biol Cell 97:629-40. 2005
    ..In the present study, we have investigated the stage-specific action and role of p38 MAPK in regulating filamentous actin during murine preimplantation development...
  35. pmc Serum albumin level correlates with disease severity in patients with Hemorrhagic Fever with Renal Syndrome
    Young Ok Kim
    Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
    J Korean Med Sci 18:696-700. 2003
    ..001), serum creatinine (r=-0.394, p<0.001), and AST (r=-0.251, p=0.002). Our data suggest that hypoalbuminemia frequently occurs in the acute stage of HFRS, and level of serum albumin is associated with the disease severity of HFRS...
  36. ncbi An amino-terminal amphipathic alpha-helix mediates membrane association of the hepatitis C virus nonstructural protein 5A
    Volker Brass
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Biol Chem 277:8130-9. 2002
    ..These findings have important implications for the structural and functional organization of the HCV replication complex and may define novel targets for antiviral intervention...
  37. pmc Prevalence and clinical significance of isotype specific antinuclear antibodies in primary biliary cirrhosis
    E I Rigopoulou
    Institute of Liver Studies, King s College Hospital, Denmark Hill, London SE5 9RS, UK
    Gut 54:528-32. 2005
    ..Antinuclear antibodies (ANA) giving a rim-like/membranous (RL/M) or a multiple nuclear dot (MND) pattern are highly specific for primary biliary cirrhosis (PBC). Aim and..
  38. ncbi The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes
    W G Whitfield
    Department of Biological Sciences, University, Dundee, UK
    J Cell Sci 108:3377-87. 1995
    ..The data suggest that the function of CP190 involves cell cycle dependent associations with both the centrosome, and with specific chromosomal loci...
  39. ncbi Degradation of mutated bovine pancreatic trypsin inhibitor in the yeast vacuole suggests post-endoplasmic reticulum protein quality control
    Christina M Coughlan
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 279:15289-97. 2004
    ..Because only the mutant protein is quantitatively proteolyzed these data suggest that a post-ER quality control check-point diverts unstable proteins to the vacuole for degradation...
  40. ncbi Prevalence of Rickettsia infection in dogs from the urban and rural areas of Monte Negro municipality, western Amazon, Brazil
    Marcelo B Labruna
    Faculty of Veterinary Medicine, University of Sao Paulo, Sao Paulo, Brazil
    Vector Borne Zoonotic Dis 7:249-55. 2007
    ..parkeri, R. amblyommii, and R. rhipicephali. While R. parkeri is a known human pathogen, further studies are required to verify the potential role of R. amblyommii and R. rhipicephali as human pathogens...
  41. ncbi Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins
    Pavneesh Madan
    Department of Obstetrics and Gynaecology, University of Western Ontario, Children s Health Research Institute Victoria Research Laboratories, London, Ontario N6A 4G5, Canada
    J Biol Chem 282:12127-34. 2007
  42. ncbi Synapsin and synaptic vesicle protein expression during embryonic and post-natal lens fiber cell differentiation
    Peter H Frederikse
    Department of Pharmacology and Physiology, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    Mol Vis 10:794-804. 2004
    ..In the present study we examine lens expression and regulation of a complement of genes associated with cargo and synaptic vesicle transport in neurons...
  43. ncbi Indirect immunofluorescence in autoimmune diseases: assessment of digital images for diagnostic purpose
    Amelia Rigon
    Department of Clinical Medicine, Immunology, and Rheumatology, University Campus Bio Medico, Rome, Italy
    Cytometry B Clin Cytom 72:472-7. 2007
    ..To pursue a high image quality without artefacts and reduce interobserver variability, this study aims at evaluating the reliability of automatically acquired digital images of IIF slides for diagnostic purposes...
  44. pmc Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 10:R131. 2008
    ..The primary goal of this study was to determine the sensitivity of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P antibodies as detected by other immunoassays...
  45. ncbi Clinical applications of antineutrophil cytoplasmic antibody testing
    Wilhelm H Schmitt
    Vth Medical Clinic Nephrology, Endocrinolgy, Rheumatolgy, University Clinic Mannheim, Faculty of Clinical Medicine of the University of Heidelberg, Mannheim, Germany
    Curr Opin Rheumatol 16:9-17. 2004
    ..This review summarizes the most recent developments in the field, identifies areas of uncertainty, and gives practical guidelines...
  46. pmc Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins
    K Redding
    Department of Biochemistry, Stanford University, California 94305, USA
    Mol Cell Biol 16:6208-17. 1996
    ..soi1 mutants also exhibited a recessive defect in vacuolar protein sorting. Suppressor alleles of S0I2 were dominant. These results suggest that the SOI1 and S0I2 genes encode regulators or components of the TLS recognition machinery...
  47. ncbi Mitogen-activated protein kinase (MAPK) blockade of bovine preimplantation embryogenesis requires inhibition of both p38 and extracellular signal-regulated kinase (ERK) pathways
    Pavneesh Madan
    Department of Physiology and Pharmacology, University of Western Ontario, Child Health Research Institute, 5th Floor Victoria Research Laboratories, 800 Commissioners Road East, London, Ontario, Canada, N6A 4G5
    Reproduction 130:41-51. 2005
    ..We have determined that, in variance to what was observed during murine preimplantation development, bovine early embryos progress at normal frequencies to the blastocyst stage in the presence of p38 MAPK inhibitors...
  48. ncbi Methimazole-induced antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis and lupus-like syndrome with a cutaneous feature of vesiculo-bullous systemic lupus erythematosus
    Haw Yueh Thong
    Acta Derm Venereol 82:206-8. 2002
  49. ncbi p38 MAPK signaling during murine preimplantation development
    David R Natale
    Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1
    Dev Biol 268:76-88. 2004
    ..This study demonstrates that p38 MAPK activity is required to support development through the murine preimplantation interval...
  50. pmc A novel nuclear structure containing the survival of motor neurons protein
    Q Liu
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    EMBO J 15:3555-65. 1996
    ..These structures are similar in number (2-6) and size (0.1-1.0 micron) to coiled bodies, and frequently are found near or associated with coiled bodies. We term these prominent nuclear structures gems, for Gemini of coiled bodies...
  51. ncbi Propylthiouracil and carbimazole associated-antineutrophil cytoplasmic antibodies (ANCA) in patients with Graves' disease
    L Harper
    Division of Medical Sciences, University of Birmingham, Edgbaston, Birmingham, UK
    Clin Endocrinol (Oxf) 60:671-5. 2004
    ..We aimed to investigate whether carbimazole therapy was also associated with increased risk of ANCA...
  52. pmc Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation
    Barry Fong
    Department of Physiology and Pharmacology, The University of Western Ontario London, Ontario, Canada
    BMC Dev Biol 7:2. 2007
  53. ncbi [Comparison of techniques used to screen blood donations for malaria antibodies]
    C P Soler
    Service de qualification biologique du don, Centre de Transfusion Sanguine des Armées, 1 Rue du Lieutenant Batany, BP 140, 92141 Clamart, France
    Med Trop (Mars) 63:587-9. 2003
    ..The exception is malaria antibody detection that is performed by the indirect fluorescent antibody technique using commercially available kits...
  54. pmc A virosomal malaria peptide vaccine elicits a long-lasting sporozoite-inhibitory antibody response in a phase 1a clinical trial
    Shinji L Okitsu
    Molecular Immunology, Swiss Tropical Institute, Basel, Switzerland
    PLoS ONE 2:e1278. 2007
    ..The main objective of this study was to perform a detailed immunological and functional analysis of the CSP-specific antibodies elicited in this phase 1a trial...
  55. ncbi [Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing]
    Xiao Ran Yang
    National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
    Zhonghua Liu Xing Bing Xue Za Zhi 28:688-91. 2007
    ..To detect Bartonella henselae IgG antibody among healthy people in Changping, Beijing...
  56. pmc The nuclear pore complex protein Tpr is a common autoantigen in sera that demonstrate nuclear envelope staining by indirect immunofluorescence
    Y Ou
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Calgary, Calgary, AB, Canada
    Clin Exp Immunol 136:379-87. 2004
    ..This report indicates that Tpr, a component of the NuPC, is a common target of human autoantibodies that react with the NE...
  57. pmc Ste6p mutants defective in exit from the endoplasmic reticulum (ER) reveal aspects of an ER quality control pathway in Saccharomyces cerevisiae
    D Loayza
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Biol Cell 9:2767-84. 1998
  58. ncbi Hominoid-specific SPANXA/D genes demonstrate differential expression in individuals and protein localization to a distinct nuclear envelope domain during spermatid morphogenesis
    V A Westbrook
    Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, VA 22908, USA
    Mol Hum Reprod 12:703-16. 2006
  59. pmc Culturing MDCK cells in three dimensions for analyzing intracellular dynamics
    Natalie Elia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2009
    ..This unit discusses technical aspects for culturing and imaging MDCK 3-D culture for both fixed 3-D cultures and live-cell imaging...
  60. ncbi Evaluation of the BioPlex 2200 ANA screen: analysis of 510 healthy subjects: incidence of natural/predictive autoantibodies
    O Shovman
    Department of Medicine B and Center for Autoimmune Diseases, Chaim Sheba Medical Center, Tel Hashomer 52621, Israel
    Ann N Y Acad Sci 1050:380-8. 2005
    ..252) to the ELISA ANA screening test. Like the ELISA, the BioPlex 2200 has a lower (P < 0.001) positive rate than IIF for the autoantibody screening...
  61. pmc Contrasting roles of endosomal pH and the cytoskeleton in infection of human glial cells by JC virus and simian virus 40
    Aarthi Ashok
    Department of Molecular Microbiology and Immunology, Brown University, Providence, Rhode Island 02912, USA
    J Virol 77:1347-56. 2003
    ..These data also suggest a unique sequential involvement of cytoskeletal elements during infection of glial cells by JCV...
  62. pmc Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex
    R Bastos
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 137:989-1000. 1997
    ..In this way, Nup84 could play a central role in the organization of the interface between the pore complex and the cytoplasm...
  63. ncbi Caspr2, a new member of the neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels
    S Poliak
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Neuron 24:1037-47. 1999
    ..This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains...
  64. pmc Stress tolerance of misfolded carboxypeptidase Y requires maintenance of protein trafficking and degradative pathways
    Eric D Spear
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Mol Biol Cell 14:2756-67. 2003
    ..Expression of CPY* at levels tolerated by wild-type cells was toxic to these strains despite retaining the ability to activate the UPR...
  65. ncbi The in vitro and in vivo proliferative capacity of serum-free cultivated human conjunctival epithelial cells
    Leonard P K Ang
    Singapore National Eye Center, Singapore
    Curr Eye Res 28:307-17. 2004
    ..To investigate the in vitro and in vivo proliferative capacity of human conjunctival epithelial cells cultured in serum-free media, and to compare this with current methods that utilize serum-containing media and 3T3 feeder layers...
  66. pmc Dbp6p is an essential putative ATP-dependent RNA helicase required for 60S-ribosomal-subunit assembly in Saccharomyces cerevisiae
    D Kressler
    Departement de Biochimie Medicale, Centre Medical Universitaire, Universite de Geneve, Geneva, Switzerland
    Mol Cell Biol 18:1855-65. 1998
    ..We propose that Dbp6p is required for the proper assembly of preribosomal particles during the biogenesis of 60S ribosomal subunits, probably by acting as an rRNA helicase...
  67. pmc Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains
    C C F C Laurino
    Rheumatology Division, Universidade Federal de Sao Paulo, Escola Paulista de Medicina Unifesp EPM, Rua Botucatu 740, Sao Paulo, SP 04023 062, Brazil
    Clin Exp Immunol 143:572-84. 2006
    ..The clinical association of CDS-1 sera included a variety of systemic and organ-specific autoimmune diseases but they were also observed in patients with no evidence of autoimmune disease...
  68. pmc Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly
    P Grandi
    Biochemie Zentrum Heidelberg BZH, University of Heidelberg, Germany
    Mol Biol Cell 8:2017-38. 1997
  69. pmc Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein
    H J Matthies
    Division of Cellular and Molecular Medicine, University of California at an San Diego 92093 0683, USA
    J Cell Biol 134:455-64. 1996
    ..Based on these observations, we propose that microtubule bundling by the NCD kinesin-like protein promotes assembly of a stable bipolar spindle in the absence of typical MTOCs...
  70. ncbi Spotted fever in Brazil: a seroepidemiological study and description of clinical cases in an endemic area in the state of São Paulo
    E R de Lemos
    Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil
    Am J Trop Med Hyg 65:329-34. 2001
    ..Twenty-two (4.2%) of these samples demonstrated titers > or = 1:64. The results indicate that Brazilian spotted fever is endemic within this region of Brazil...
  71. ncbi Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants
    M S Toledo
    Department of Biochemistry, , Rua Botucatu 862, , SP, 04023-900, Brazil
    Glycobiology 11:105-12. 2001
    ..fumigatus was also observed. The availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed...
  72. pmc Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p
    J D Aitchison
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021, USA
    J Cell Biol 131:1133-48. 1995
  73. ncbi PKC isoenzymes in the chicken lens and TPA-induced effects on intercellular communication
    V M Berthoud
    Department of Pediatrics, University of Chicago, Illinois 60637 1470, USA
    Invest Ophthalmol Vis Sci 41:850-8. 2000
  74. ncbi Profile and clinical significance of anti-nuclear envelope antibodies found in patients with primary biliary cirrhosis: a multicenter study
    Kiyomitsu Miyachi
    First Diagnostic Division, Health Sciences Research Institute Inc, 106 Godo cho Hodogaya ku, Yokohama shi, Kanagawa 240 0005, Japan
    J Autoimmun 20:247-54. 2003
    ..This difference was found to be statistically significant. The presence of anti-p62 complex antibody may be related with the progressive or advanced state of PBC...
  75. pmc Detection and identification of antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testing
    I Peene
    Department of Rheumatology, Ghent University Hospital, Belgium
    Ann Rheum Dis 60:1131-6. 2001
    ..CONCLUSIONS: The data from this analysis are useful in estimating the probabilities of detecting specific ANA. Line immunoassay was shown to be a sensitive test for the detection of anti-ENA antibodies...
  76. ncbi Antinuclear antibodies specific for primary biliary cirrhosis
    Howard J Worman
    Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, 10th Floor, Room 508, New York, NY 10032, USA
    Autoimmun Rev 2:211-7. 2003
    ..While antibodies against gp210, sp100 and some other nuclear proteins are very specific to PBC and may therefore be useful diagnostic markers, their connection to pathogenesis remains to be elucidated...
  77. ncbi Anti-NuMA antibodies: an uncommon specificity in scleroderma sera
    R Herrera-Esparza
    Department of Immunology, Universidad Autonoma de Zacatecas, Mexico
    Rev Rhum Engl Ed 66:315-8. 1999
    ..Serum antibodies in scleroderma patients are generally directed against the nucleolus and centromeres. A small proportion of patients have serum antibodies to the centrioles and mitotic apparatus...
  78. ncbi CD36-dependent adhesion and knob expression of the transmission stages of Plasmodium falciparum is stage specific
    K P Day
    Wellcome Trust Centre for Epidemiology of Infectious Disease, Department of Zoology, University of Oxford
    Mol Biochem Parasitol 93:167-77. 1998
    ..The adhesion phenotype of these young gametocytes was indistinguishable from that of the trophozoites by all criteria examined. These data support the hypothesis that other host receptors mediate the binding of late gametocytes...
  79. pmc Collection of corneal impression cytology directly on a sterile glass slide for the detection of viral antigen: an inexpensive and simple technique for the diagnosis of HSV epithelial keratitis - a pilot study
    S Athmanathan
    Jhaveri Microbiology Center, Hyderabad Eye Research Foundation, India
    BMC Ophthalmol 1:3. 2001
    ..Conventional virology techniques are often expensive and time consuming. We describe here a highly economical, simple, rapid and sensitive technique for the collection of impression cytology, for the laboratory diagnosis of HSK...
  80. ncbi alpha-Internexin is the only neuronal intermediate filament expressed in developing cerebellar granule neurons
    C L Chien
    Department of Pathology, Columbia University College of Physicans and Surgeons, New York, 10032, USA
    J Neurobiol 29:304-18. 1996
    ..Furthermore, alpha-internexin may play a key role in neurite outgrowth and the establishment of neuronal cytoarchitecture...
  81. ncbi Lipopolysaccharide-dependent induction of leech leukocytes that cross-react with vertebrate cellular differentiation markers
    M de Eguileor
    DBSF University of Insubria, Varese, Italy
    Tissue Cell 32:437-45. 2000
    ..CD61 and CD68 both recognised a band of about 110 kD; CD11b recognised a band of 200 kD, and CD11c, a band of 180 kD...
  82. ncbi Serological investigation of Bartonella henselae infections in clinically cat-scratch disease-suspected patients, patients with cardiovascular diseases, and healthy veterinary students in Japan
    Eisaku Kikuchi
    Department of Veterinary Medicine, College Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan
    Microbiol Immunol 46:313-6. 2002
    ..henselae antibody had had some contacts with cats. In CSD-suspected patients, the B. henselae positive rate in females was significantly higher than in males, and high seropositive rates to B. henselae were found in younger age groups...
  83. ncbi Differential nuclear localization of the cancer/testis-associated protein, SPAN-X/CTp11, in transfected cells and in 50% of human spermatozoa
    V A Westbrook
    Departments of Cell Biology and Obstetrics and Gynecology, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    Biol Reprod 64:345-58. 2001
  84. ncbi Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif
    R Erlich
    Department of Pathology and Immunology, Monash University Medical School, Melbourne, Victoria 3181, Australia
    J Biol Chem 271:8328-37. 1996
    ..This is the first report of a cytosolic Golgi protein containing a granin motif. The structural characteristics of p230 indicate that it may play a role in vesicular transport from the trans-Golgi...
  85. ncbi RNA interference targeting transforming growth factor-beta type II receptor suppresses ocular inflammation and fibrosis
    Hiroshi Nakamura
    Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, 60612, USA
    Mol Vis 10:703-11. 2004
    ..The purpose of the study was to determine whether small interference RNAs (siRNAs) targeting the type II receptor of TGF-beta (TbetaRII) could be used to suppress the TGF-beta action...
  86. ncbi Involvement of SMAD4, but not of SMAD2, in transforming growth factor-beta1-induced trophoblast expression of matrix metalloproteinase-2
    Hai Yan Lin
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Front Biosci 11:637-46. 2006
    ..However, retrovirus-mediated sense Smad2 gene transfer had no effect. These findings suggest that Smad4, but not Smad2, mediates TGF-beta1-induced MMP-2 expression in invasive extravillous trophoblasts...
  87. ncbi Hypoxia induces the expression of membrane-type 1 matrix metalloproteinase in retinal glial cells
    Kousuke Noda
    Department of Pathology, Keio University School of Medicine, Tokyo, Japan
    Invest Ophthalmol Vis Sci 46:3817-24. 2005
    ..However, little is known about the triggers for MT1-MMP induction in the diabetic retina. In the present study, the effect of tissue hypoxia on MT1-MMP expression in retinal glial cells was investigated...
  88. pmc Sequential molecular and cellular events during neoplastic progression: a mouse syngeneic ovarian cancer model
    Paul C Roberts
    Department of Immunology Microbiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA
    Neoplasia 7:944-56. 2005
  89. ncbi LBP and CD14 secreted in tears by the lacrimal glands modulate the LPS response of corneal epithelial cells
    David R Blais
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ontario, Canada
    Invest Ophthalmol Vis Sci 46:4235-44. 2005
    ..Better understanding of the spatial expression of the LPS receptor components at the tear-corneal interface might facilitate enhanced functions of the LPS receptor complex in ocular defense against Gram-negative infections...
  90. ncbi Plasmodium yoelii: axenic development of the parasite mosquito stages
    Johanna M Porter-Kelley
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Exp Parasitol 112:99-108. 2006
    ..Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations...
  91. ncbi Development and characterization of human constitutive proteasome and immunoproteasome subunit-specific monoclonal antibodies
    N Bandoh
    Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Tissue Antigens 66:185-94. 2005
    ..These results suggest that the constitutive proteasome and immunoproteasome subunit-specific mAbs we have developed are useful probes to characterize the expression of proteasome subunits in normal tissues and in pathological lesions...
  92. ncbi Entamoeba histolytica: identification of a distinct beta2 integrin-like molecule with a potential role in cellular adherence
    Dylan R Pillai
    Division of Infectious Diseases, Department of Medicine, University of California, San Francisco 94143, USA
    Exp Parasitol 109:135-42. 2005
    ..The presence of integrin-dependent binding may allow trophozoites to opportunistically adhere to activated intestinal epithelium or vascular endothelium expressing ICAM-1 during amebic colitis or hepatic abscess...
  93. ncbi Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka
    A Naguleswaran
    Institute of Parasitology, University of Bern, Laenggass Str 122, CH 3001 Bern, Switzerland
    Vet Parasitol 126:257-62. 2004
    ..caninum antibodies. The optimal correlation between ELISA, IFAT, and Western blot confirms the suitability of the ELISA for large-scale seroepidemiologic studies, not only in cattle but also in goats...
  94. ncbi Production and characterization of a monoclonal antibody specific for NS3 protease and the ATPase region of Dengue-2 virus
    J Garcia-Cordero
    Departamento de Biomedicina Molecular, CINVESTAV IPN, Mexico City, Mexico
    Hybridoma (Larchmt) 24:160-4. 2005
    ..We conclude that these antibodies may be a useful tool, not only to study the replicative process of Dengue virus, but also to generate specific diagnostic tools for Dengue infection...
  95. ncbi Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors
    Gabriela Kania
    Institute of Plant Genetics and Crop Plant Research IPK, Corrensstr 3, D 06466 Gatersleben, Germany
    Stem Cells 23:791-804. 2005
    ..In conclusion, we present the somatic stem cell marker prominin-1 as a new parameter to define ES-derived committed and early progenitor cells...
  96. ncbi Icariin-mediated modulation of cell cycle and p53 during cardiomyocyte differentiation in embryonic stem cells
    Danyan Zhu
    Department of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China
    Eur J Pharmacol 514:99-110. 2005
    ..Results showed p53 to be an important regulator in the differentiation in embryonic stem cells treated with 10(-7) M icariin, controlling or adjusting the balance between differentiated cells and cells undergoing apoptosis...
  97. pmc Expression of macrophage colony-stimulating factor receptor is increased in the AbetaPP(V717F) transgenic mouse model of Alzheimer's disease
    G M Murphy
    Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, California 94305 5485, USA
    Am J Pathol 157:895-904. 2000
    ..Because chronic activation of microglia is thought to result in neuronal injury, the M-CSF system may be a potential target for therapeutic intervention in AD...
  98. ncbi Novel splicing variant of mouse Orc1 is deficient in nuclear translocation and resistant for proteasome-mediated degradation
    Yasuyuki Miyake
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:12643-52. 2005
  99. ncbi Rapid apoptosis induction by IGFBP-3 involves an insulin-like growth factor-independent nucleomitochondrial translocation of RXRalpha/Nur77
    Kuk Wha Lee
    Division of Pediatric Endocrinology, Mattel Children s Hospital at UCLA, David Geffen School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 280:16942-8. 2005
    ..This unique nongenotropic nuclear pathway supports an emerging role for IGFBP-3 as a novel, multicompartmental signaling molecule involved in induction of apoptosis in malignant cells...
  100. ncbi PAF-induced furin and MT1-MMP expression is independent of MMP-2 activation in corneal myofibroblasts
    Paulo Ottino
    Department of Ophthalmology and Neuroscience Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
    Invest Ophthalmol Vis Sci 46:487-96. 2005
    ..Corneal stromal myofibroblasts express the platelet-activating factor (PAF) receptor, but its role is unclear. In the present study, the effect of PAF on induction of metalloproteinases (MMPs) was investigated...
  101. ncbi Tissue-type plasminogen activator acts as a cytokine that triggers intracellular signal transduction and induces matrix metalloproteinase-9 gene expression
    Kebin Hu
    Department of Pathology, University of Pittsburgh School of Medicine, S 405 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    J Biol Chem 281:2120-7. 2006

Research Grants62

  1. Hospital and Household Studies of Respiratory Cryptosporidiosis and Transmission
    Jeffrey K Griffiths; Fiscal Year: 2013
    ..We wish to see if it can be spread from, or to, the lungs, like influenza or tuberculosis. If it can spread in this way, then we can prevent people from getting infected by actions such as wearing masks and washing hands after coughing. ..
  2. L2DTL Function in Maintaining Genomic Stability during Drosophila Development
    CATHERINE SILVER KEY; Fiscal Year: 2010
    ..Experiment results should support the hypothesis that L2DTL is essential for preventing re-replication in all cell contexts. ..
  3. Documentation of Novel Keratoconus Markers: In Vitro and In Vivo
    Dimitrios Karamichos; Fiscal Year: 2013
    ..Also, earlier detection of forme fruste keratoconus may lead to earlier intervention. ..
  4. Rapid point of care HIV PCR diagnostic device
    JOHN CHARLES GERDES; Fiscal Year: 2010
    ..We propose the miniaturization and automation of nucleic acid testing methods to enable low cost, near patient testing. ..
  5. Investigating the Role of Malaria Rhoptry-Associated Protein 2 in Severe Anemia
    NOE DAVID GOMEZ; Fiscal Year: 2010
    ..The full mechanism of disease associated with SMA is unknown. This study plans to shed light on how rhoptry-associated protein 2 (RAP2) contributes to the presentation of SMA. ..
    DON GRAVES; Fiscal Year: 1980
    ..It is also possible that there exists more than one strain of BLV and these studies may give suggestive evidence of this...
    ROBERT DEPHILIP; Fiscal Year: 1991
    ..This information should find immediate application in the consideration of spermatogenic dysfunction and in the design of protocols able to safely regulate male germ cell development...
    Manuel Buchwald; Fiscal Year: 1999
    ..The fifth aim is to isolate the FAE gene by the expression cloning used so successfully to date. ..
  9. Immunofluorescence Standards for Flow Cytometry
    Douglas Redelman; Fiscal Year: 2003
    ..abstract_text> ..
    Bruce Bochner; Fiscal Year: 1993
    Jorgen Slots; Fiscal Year: 1990
    ..The findings may lead to the formulation of new and more effective means of diagnosis, prevention and treatment of human periodontitis...
  12. Generation of Reagents for E. bieneusi Genome Sequencing
    Saul Tzipori; Fiscal Year: 2003
    ..The outcome of this proposal will lead to a submission of a full proposal to sequence the EB genome. ..
    JOEL EISSENBERG; Fiscal Year: 1992
    Regina Santella; Fiscal Year: 1991
    ..Levels of the different biological markers will be correlated with exposure. These studies will thus develop and validate methods to monitor the biological effects of human exposure to PAHs and serve as a model system for skin exposure...
  15. Adenosine 3',5'-cyclic monophosphate signaling in Mycobacterium tuberculosis
    Amanda Pendleton; Fiscal Year: 2009
    ..PUBLIC HEALTH RELEVANCE: Approximately two million people die each year from infection with Mtb. The identification of new Mtb drug targets and improved treatment strategies are sorely needed to control this emerging epidemic. ..
    George Wright; Fiscal Year: 1990
    Joseph Frankel; Fiscal Year: 1993
    ..though the causes are many, final developmental paths...are few indeed" (Opitz, 1985, Amer. J. Med. Genet. 21, 1-11). The proposed research is concerned with understanding general rules that constrain "final developmental paths"...
  18. Identifying Aspergillus adhesins that bind ECM proteins
    Diana Bartelt; Fiscal Year: 2006
    ..These infections can be fatal. There are very few effective anti-fungal drugs. Proteins that cause spores to bind to tissue could be important targets in the development of anti-fungal therapies. [unreadable] [unreadable] [unreadable]..
    JENNIFER MC KNIGHT; Fiscal Year: 1992
    LYNN WILEY; Fiscal Year: 1980
    STEVEN BRADWAY; Fiscal Year: 1990
    ..albicans surface components will be studied. This will be accomplished using purified salivary components derivatized with iodinated reversible photoaffinity crosslinking agents...
    Sudhir Gupta; Fiscal Year: 1990
    ..The studied would help in the understanding of the mechanisms of CsA on the early steps of lymphocyte activation and the mechanisms by which CsA corrects the pleiotropic drug resistance in neoplastic cells...
  23. Role of Spinal GABA Receptors in Neuropathic Pain
    Donna Hammond; Fiscal Year: 2006
    ..abstract_text> ..
    Molly Kulesz Martin; Fiscal Year: 2002
    ..Knowledge of different functional p53 protein forms in cells and cooperating factors may improve the resolution, prognosis or treatment plan for human tumors with selective loss of distinct p53 functions. ..
    FREDERICK HUGHSON; Fiscal Year: 2002
    ..These studies will provide the first insights into the role of this novel protein complex in the process of intracellular protein traffic. ..
    Henry Donahue; Fiscal Year: 2000
    ..The results of this project will provide insights into the mechanism by which biophysical signals regulate chondrocyte metabolism. ..
    ROGER SAWYER; Fiscal Year: 1990
    ..These studies are of particular importance to the understanding and control of the numerous skin disorders, affecting the human population...
  28. Malaria antigens binding to band 3 in red cell invasion
    Steven Oh; Fiscal Year: 2004
    ..falciparum malaria. ..
    Becky Marquez; Fiscal Year: 2004
    ..We propose that hyperactivation is mediated by a signaling pathway involving phosphorylation of flagellar proteins, which is dependent on Ca 2+ influx and alkalization of the cytosol. ..
    C Bell; Fiscal Year: 1980
    ..4. To determine the relationships of the occurrence of these parameters to the different stages of human lung cancer...
    Frank Yin; Fiscal Year: 2002
    ..Once these goals are achieved, we will have the tools to begin answering some crucial questions about cell function using AFM. ..
  32. Inducible Huntingtin Expression in a Neuronal Like Cell Model System
    Ricky Hirschhorn; Fiscal Year: 2009
    ..Characterization of these cell lines will advance understanding of the pathologic mechanism leading to the disease state and provide cell model systems to screen potential therapeutics in future studies. ..
    Christina Rogers; Fiscal Year: 1980
    ..Experiments will be conducted to determine optimum conditions for growth of recombinant DNA-containing cells, and for transfection of mouse fibroblasts and other eukaryotic cells. ..
    Gerald Litwack; Fiscal Year: 1992
    ..The genetically pure glucocorticoid receptor will be expressed in large quantity in an expression vector in order to assess its functions and for structural studies as well as use in reassembling the 320 kDa complex...
    PAUL BENYA; Fiscal Year: 1991
    ..Developing an understanding of the mechanisms of phenotypic change, using this unique model system, will greatly enhance the probability of future clinical intervention at natural control points...
  36. Identifying cellular genes as anti-HIV drug targets
    Ashlee Moses; Fiscal Year: 2003
    ..The company intends to develop intellectual property protection for the use of novel validated genes in antiviral drug assays, and pursue the development of such drugs. ..
    AHVIE HERSHKOWITZ; Fiscal Year: 1993
    ..It takes advantage of new technology and established collaborations in cardiology, immunology, pathology and virology to study the immunopathogenesis of AIDS-associated cardiomyopathy...
  38. Regulation of Mammalian Oocyte Maturation
    Stephen Downs; Fiscal Year: 2007
    ..Furthermore, these studies will benefit the development of in vitro systems where either meiotic arrest or completion of meiotic maturation is the desired endpoint. ..
    Christina Teng; Fiscal Year: 1980
    ..In tissue culture, the steroid hormone production and the gonadotropic hormone effect on epithelial-like cells obtained after density gradient separation will continue to be investigated...
  40. Regulation of PTEN Tumor Suppressor
    Andrew Chan; Fiscal Year: 2006
    ..In addition, novel signaling pathways will be delineated and will provide targets for tumor diagnosis and therapeutic interventions. ..
    Steven Hanks; Fiscal Year: 1991
    ..A major thrust of the proposed studies will be to further explore the structural and functional relationships between these proteins...
    HARRY IOACHIM; Fiscal Year: 1980
    ..The aim of this project is to study the mechanisms of antigenic modulation of leukemia cells and its correlations with tumor growth...
    Rajesh Gandhi; Fiscal Year: 2002
    Michael Forster; Fiscal Year: 1990
  45. Screening Small Molecules for Rescue of Peroxisome Assembly Defects
    Steven Steinberg; Fiscal Year: 2007
    ..Identified compounds will be tested in cell lines from patients with other known defects to determine broad applicability. Ultimately these drugs could be developed for patient use. [unreadable] [unreadable] [unreadable]..
    Joseph Culotti; Fiscal Year: 1991
    ..There is good reason to believe that at least some of the molecular mechanisms used for pioneer neuron guidance in C. elegans may be conserved in vertebrates. Eventually we plan to examine this possibility...
    DAVID BING; Fiscal Year: 1990
    ..The results of these studies (Phase 1) will indicate whether human lymphocyte transformation by retroviruses correlates with the expression of aberrant PDGFR-B mRNAs of different sizes and consequently different PDGFR- B proteins...
    JAMES CASELLA; Fiscal Year: 1991
    ..The long term goal of this proposal is to develop an understanding of how the length, number, and attachments of actin filaments to other structures are regulated...
    Rebecca Kohn; Fiscal Year: 1999
    ..Proper neurotransmitter release is crucial to proper function of the nervous system, and defective neurotransmitter release has been implicated in many psychiatric and neurobiological disorders. ..
  50. Proteomic Analysis of Retinal Ganglion Cell Death in Glaucoma
    GULGUN TEZEL; Fiscal Year: 2010
    ..These studies are expected to provide comprehensive information about the nerve cell death process, thereby allowing new treatment possibilities for 3 million Americans suffering from glaucoma. ..
  51. VTC Events Mediated by the Rab2 Complex
    Ellen J Tisdale; Fiscal Year: 2010
    ..The studies proposed in this application will develop a new and novel paradigm that couples Rab2-Apkc lA-GAPDH dysfunction with pathological consequences. ..
    Nazzareno Ballatori; Fiscal Year: 2007
  53. Proteomic Analyses of Human Trabecular Meshwork
    Sanjoy Bhattacharya; Fiscal Year: 2007
    ..The long-term goal of this proposal is to better understand mechanisms of glaucoma pathogenesis and facilitate the development of effective therapies for limiting disease progression. [unreadable] [unreadable]..
    Nazzareno Ballatori; Fiscal Year: 2005
    ..Evaluate the molecular mechanism by which NAC stimulates renal excretion of MeHg. IV. Test the hypothesis that urinary excretion of MeHg following an NAC oral challenge may be used as a new biomarker of MeHg exposure. ..
  55. Elevated potassium ion in lacrimal fluid and the health of the ocular surface
    John Ubels; Fiscal Year: 2009
    ..If the high potassium in tears is important for the health of the ocular surface, this information will be useful in the development of improved treatments for dry eye disease. ..
    John Ubels; Fiscal Year: 2002
    ..abstract_text> ..
  57. Gene Gun Technology, Opioids, and Corneal Diseases
    IAN ZAGON; Fiscal Year: 2003
    ..Ultimately, such data can be employed to design molecular strategies to remedy visual dysfunction. ..
  58. Analysis of Lacrimal Gland by Laser Microdissection
    John Ubels; Fiscal Year: 2003
    ..abstract_text> ..
  59. Muller glia and neuronal regeneration in the retina
    Andy Fischer; Fiscal Year: 2009
    ..This information could be applied to not only stimulate neural regeneration to treat sight-threatening diseases of the retina, but also to control gliosis that may be detrimental to the pathogenesis of retinal disorders. ..
    Stuart Meyers; Fiscal Year: 2004
    ..Dobrinski and experiments involving germ cell transplantation into male macaques will be performed at UC Davis. We expect to expand this study into a fully-independent project. ..
  61. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010