indirect fluorescent antibody technique

Summary

Summary: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)

Top Publications

  1. ncbi Outbreak of cutaneous leishmaniasis in northern Israel
    Raymond L Jacobson
    Department of Parasitology, The Kuvin Center for Study of Infectious and Tropical Diseases, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Infect Dis 188:1065-73. 2003
  2. pmc Prevalence and clinical significance of isotype specific antinuclear antibodies in primary biliary cirrhosis
    E I Rigopoulou
    Institute of Liver Studies, King s College Hospital, Denmark Hill, London SE5 9RS, UK
    Gut 54:528-32. 2005
  3. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
  4. pmc Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body
    Jiang Hong Yu
    Department of Medicine, Havard Medical School, the Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Charlestown, MA 02129, USA
    RNA 11:1795-802. 2005
  5. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  6. doi Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells
    Rico Hiemann
    Department of Biology, Chemistry and Process Technology, Lausitz University of Applied Sciences, Senftenberg, Germany
    Autoimmun Rev 9:17-22. 2009
  7. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
  8. ncbi A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis
    Vanda A Lennon
    Department of Neurology, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Lancet 364:2106-12. 2004
  9. ncbi [Comparison of techniques used to screen blood donations for malaria antibodies]
    C P Soler
    Service de qualification biologique du don, Centre de Transfusion Sanguine des Armées, 1 Rue du Lieutenant Batany, BP 140, 92141 Clamart, France
    Med Trop (Mars) 63:587-9. 2003
  10. ncbi Mitogen-activated protein kinase (MAPK) blockade of bovine preimplantation embryogenesis requires inhibition of both p38 and extracellular signal-regulated kinase (ERK) pathways
    Pavneesh Madan
    Department of Physiology and Pharmacology, University of Western Ontario, Child Health Research Institute, 5th Floor Victoria Research Laboratories, 800 Commissioners Road East, London, Ontario, Canada, N6A 4G5
    Reproduction 130:41-51. 2005

Research Grants

Detail Information

Publications217 found, 100 shown here

  1. ncbi Outbreak of cutaneous leishmaniasis in northern Israel
    Raymond L Jacobson
    Department of Parasitology, The Kuvin Center for Study of Infectious and Tropical Diseases, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Infect Dis 188:1065-73. 2003
    ..tropica foci. P. arabicus is the main vector, and it transmits parasites that are different from other L. tropica isolates, with respect to antigenic, molecular, and biochemical parameters...
  2. pmc Prevalence and clinical significance of isotype specific antinuclear antibodies in primary biliary cirrhosis
    E I Rigopoulou
    Institute of Liver Studies, King s College Hospital, Denmark Hill, London SE5 9RS, UK
    Gut 54:528-32. 2005
    ..Antinuclear antibodies (ANA) giving a rim-like/membranous (RL/M) or a multiple nuclear dot (MND) pattern are highly specific for primary biliary cirrhosis (PBC). Aim and..
  3. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  4. pmc Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body
    Jiang Hong Yu
    Department of Medicine, Havard Medical School, the Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Charlestown, MA 02129, USA
    RNA 11:1795-802. 2005
    ..Taken together, the results of this study show that Ge-1 is a central component of P-bodies and suggest that Ge-1 may act prior to the 5(')-decapping step in mRNA degradation...
  5. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
    ....
  6. doi Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells
    Rico Hiemann
    Department of Biology, Chemistry and Process Technology, Lausitz University of Applied Sciences, Senftenberg, Germany
    Autoimmun Rev 9:17-22. 2009
    ..More sophisticated pattern recognition algorithms and novel calibration systems will improve standardised quantifications of IIF image interpretation...
  7. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
    ..The three phases of methylation reprogramming may have roles in imprinting, the control of gene expression, and the establishment of nuclear totipotency...
  8. ncbi A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosis
    Vanda A Lennon
    Department of Neurology, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Lancet 364:2106-12. 2004
    ..We assessed the capacity of a putative marker for neuromyelitis optica (NMO-IgG) to distinguish neuromyelitis optica and related disorders from multiple sclerosis...
  9. ncbi [Comparison of techniques used to screen blood donations for malaria antibodies]
    C P Soler
    Service de qualification biologique du don, Centre de Transfusion Sanguine des Armées, 1 Rue du Lieutenant Batany, BP 140, 92141 Clamart, France
    Med Trop (Mars) 63:587-9. 2003
    ..The exception is malaria antibody detection that is performed by the indirect fluorescent antibody technique using commercially available kits...
  10. ncbi Mitogen-activated protein kinase (MAPK) blockade of bovine preimplantation embryogenesis requires inhibition of both p38 and extracellular signal-regulated kinase (ERK) pathways
    Pavneesh Madan
    Department of Physiology and Pharmacology, University of Western Ontario, Child Health Research Institute, 5th Floor Victoria Research Laboratories, 800 Commissioners Road East, London, Ontario, Canada, N6A 4G5
    Reproduction 130:41-51. 2005
    ..We have determined that, in variance to what was observed during murine preimplantation development, bovine early embryos progress at normal frequencies to the blastocyst stage in the presence of p38 MAPK inhibitors...
  11. ncbi In situ identification of mycobacteria in Crohn's disease patient tissue using confocal scanning laser microscopy
    S A Naser
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando 32816, USA
    Mol Cell Probes 16:41-8. 2002
    ..This technique adds an additional approach to investigate MAP role in CD etiology especially when the culture approach is long and inconsistent...
  12. ncbi Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion
    Stuart D Blacksell
    Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand
    Clin Infect Dis 44:391-401. 2007
    ..We suggest that the diagnosis of scrub typhus using IFA should be based on a > or =4-fold increase in the titer in paired serum samples and should only be based on a single sample titer when there is an adequate local evidence base...
  13. pmc Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins
    K Redding
    Department of Biochemistry, Stanford University, California 94305, USA
    Mol Cell Biol 16:6208-17. 1996
    ..soi1 mutants also exhibited a recessive defect in vacuolar protein sorting. Suppressor alleles of S0I2 were dominant. These results suggest that the SOI1 and S0I2 genes encode regulators or components of the TLS recognition machinery...
  14. pmc Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex
    R Bastos
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 137:989-1000. 1997
    ..In this way, Nup84 could play a central role in the organization of the interface between the pore complex and the cytoplasm...
  15. ncbi The heterotrimeric motor protein kinesin-II localizes to the midpiece and flagellum of sea urchin and sand dollar sperm
    J H Henson
    Department of Biology, Dickinson College, Carlisle, Pennsylvania 17013, USA
    Cell Motil Cytoskeleton 38:29-37. 1997
    ..We hypothesize that kinesin-II in the sperm may play functional roles in intraflagellar transport and/or the formation of flagella during spermatogenesis...
  16. ncbi Prevalence of Rickettsia infection in dogs from the urban and rural areas of Monte Negro municipality, western Amazon, Brazil
    Marcelo B Labruna
    Faculty of Veterinary Medicine, University of Sao Paulo, Sao Paulo, Brazil
    Vector Borne Zoonotic Dis 7:249-55. 2007
    ..parkeri, R. amblyommii, and R. rhipicephali. While R. parkeri is a known human pathogen, further studies are required to verify the potential role of R. amblyommii and R. rhipicephali as human pathogens...
  17. ncbi The development of a serum-free derived bioengineered conjunctival epithelial equivalent using an ultrathin poly(epsilon-caprolactone) membrane substrate
    Leonard P K Ang
    Singapore National Eye Center, Singapore
    Invest Ophthalmol Vis Sci 47:105-12. 2006
    ..To evaluate the use of an ultrathin poly(epsilon-caprolactone) (PCL) membrane as a substrate for the development of a serum-free-derived conjunctival epithelial equivalent...
  18. ncbi Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins
    Pavneesh Madan
    Department of Obstetrics and Gynaecology, University of Western Ontario, Children s Health Research Institute Victoria Research Laboratories, London, Ontario N6A 4G5, Canada
    J Biol Chem 282:12127-34. 2007
    ....
  19. ncbi An amino-terminal amphipathic alpha-helix mediates membrane association of the hepatitis C virus nonstructural protein 5A
    Volker Brass
    Department of Medicine II, University of Freiburg, D 79106 Freiburg, Germany
    J Biol Chem 277:8130-9. 2002
    ..These findings have important implications for the structural and functional organization of the HCV replication complex and may define novel targets for antiviral intervention...
  20. ncbi Clinical applications of antineutrophil cytoplasmic antibody testing
    Wilhelm H Schmitt
    Vth Medical Clinic Nephrology, Endocrinolgy, Rheumatolgy, University Clinic Mannheim, Faculty of Clinical Medicine of the University of Heidelberg, Mannheim, Germany
    Curr Opin Rheumatol 16:9-17. 2004
    ..This review summarizes the most recent developments in the field, identifies areas of uncertainty, and gives practical guidelines...
  21. pmc Culturing MDCK cells in three dimensions for analyzing intracellular dynamics
    Natalie Elia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2009
    ..This unit discusses technical aspects for culturing and imaging MDCK 3-D culture for both fixed 3-D cultures and live-cell imaging...
  22. pmc A novel nuclear structure containing the survival of motor neurons protein
    Q Liu
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    EMBO J 15:3555-65. 1996
    ..These structures are similar in number (2-6) and size (0.1-1.0 micron) to coiled bodies, and frequently are found near or associated with coiled bodies. We term these prominent nuclear structures gems, for Gemini of coiled bodies...
  23. ncbi Evaluation of the BioPlex 2200 ANA screen: analysis of 510 healthy subjects: incidence of natural/predictive autoantibodies
    O Shovman
    Department of Medicine B and Center for Autoimmune Diseases, Chaim Sheba Medical Center, Tel Hashomer 52621, Israel
    Ann N Y Acad Sci 1050:380-8. 2005
    ..252) to the ELISA ANA screening test. Like the ELISA, the BioPlex 2200 has a lower (P < 0.001) positive rate than IIF for the autoantibody screening...
  24. ncbi The in vitro and in vivo proliferative capacity of serum-free cultivated human conjunctival epithelial cells
    Leonard P K Ang
    Singapore National Eye Center, Singapore
    Curr Eye Res 28:307-17. 2004
    ..To investigate the in vitro and in vivo proliferative capacity of human conjunctival epithelial cells cultured in serum-free media, and to compare this with current methods that utilize serum-containing media and 3T3 feeder layers...
  25. ncbi Synapsin and synaptic vesicle protein expression during embryonic and post-natal lens fiber cell differentiation
    Peter H Frederikse
    Department of Pharmacology and Physiology, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    Mol Vis 10:794-804. 2004
    ..In the present study we examine lens expression and regulation of a complement of genes associated with cargo and synaptic vesicle transport in neurons...
  26. ncbi Propylthiouracil and carbimazole associated-antineutrophil cytoplasmic antibodies (ANCA) in patients with Graves' disease
    L Harper
    Division of Medical Sciences, University of Birmingham, Edgbaston, Birmingham, UK
    Clin Endocrinol (Oxf) 60:671-5. 2004
    ..We aimed to investigate whether carbimazole therapy was also associated with increased risk of ANCA...
  27. ncbi Hominoid-specific SPANXA/D genes demonstrate differential expression in individuals and protein localization to a distinct nuclear envelope domain during spermatid morphogenesis
    V A Westbrook
    Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, VA 22908, USA
    Mol Hum Reprod 12:703-16. 2006
    ....
  28. pmc The nuclear pore complex protein Tpr is a common autoantigen in sera that demonstrate nuclear envelope staining by indirect immunofluorescence
    Y Ou
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Calgary, Calgary, AB, Canada
    Clin Exp Immunol 136:379-87. 2004
    ..This report indicates that Tpr, a component of the NuPC, is a common target of human autoantibodies that react with the NE...
  29. pmc Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation
    Barry Fong
    Department of Physiology and Pharmacology, The University of Western Ontario London, Ontario, Canada
    BMC Dev Biol 7:2. 2007
    ....
  30. ncbi p38 mitogen-activated protein kinase (MAPK) first regulates filamentous actin at the 8-16-cell stage during preimplantation development
    Andrew J M Paliga
    Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1
    Biol Cell 97:629-40. 2005
    ..In the present study, we have investigated the stage-specific action and role of p38 MAPK in regulating filamentous actin during murine preimplantation development...
  31. pmc Serum albumin level correlates with disease severity in patients with Hemorrhagic Fever with Renal Syndrome
    Young Ok Kim
    Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
    J Korean Med Sci 18:696-700. 2003
    ..001), serum creatinine (r=-0.394, p<0.001), and AST (r=-0.251, p=0.002). Our data suggest that hypoalbuminemia frequently occurs in the acute stage of HFRS, and level of serum albumin is associated with the disease severity of HFRS...
  32. ncbi Anti-aquaporin-4 antibody is involved in the pathogenesis of NMO: a study on antibody titre
    Toshiyuki Takahashi
    Department of Neurology, Tohoku University Graduate School of Medicine, 1 1 Seiryomachi, Aobaku, Sendai 980 8574, Japan
    Brain 130:1235-43. 2007
    ....
  33. doi Aquaporin-4 antibodies in neuromyelitis optica and longitudinally extensive transverse myelitis
    Patrick Waters
    Neurosciences Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England
    Arch Neurol 65:913-9. 2008
    ..There is increasing recognition of antibody-mediated immunotherapy-responsive neurologic diseases and a need for appropriate immunoassays...
  34. ncbi [Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing]
    Xiao Ran Yang
    National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
    Zhonghua Liu Xing Bing Xue Za Zhi 28:688-91. 2007
    ..To detect Bartonella henselae IgG antibody among healthy people in Changping, Beijing...
  35. ncbi Detection of anti-SSA antibodies by indirect immunofluorescence
    Xavier Bossuyt
    Laboratory Medicine and Internal Medicine, University Hospital Leuven, Leuven, Belgium
    Clin Chem 50:2361-9. 2004
    ..We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA...
  36. ncbi Indirect immunofluorescence in autoimmune diseases: assessment of digital images for diagnostic purpose
    Amelia Rigon
    Department of Clinical Medicine, Immunology, and Rheumatology, University Campus Bio Medico, Rome, Italy
    Cytometry B Clin Cytom 72:472-7. 2007
    ..To pursue a high image quality without artefacts and reduce interobserver variability, this study aims at evaluating the reliability of automatically acquired digital images of IIF slides for diagnostic purposes...
  37. ncbi The clinical significance of antinucleolar antibodies
    S Khan
    Path Links Immunology, Scunthorpe General Hospital, Scunthorpe, UK
    J Clin Pathol 61:283-6. 2008
    ..The importance of antinucleolar antibodies seen by indirect immunofluorescence on HEp-2 cells, although associated with systemic sclerosis (SSc), in unselected patients is unknown...
  38. pmc A virosomal malaria peptide vaccine elicits a long-lasting sporozoite-inhibitory antibody response in a phase 1a clinical trial
    Shinji L Okitsu
    Molecular Immunology, Swiss Tropical Institute, Basel, Switzerland
    PLoS ONE 2:e1278. 2007
    ..The main objective of this study was to perform a detailed immunological and functional analysis of the CSP-specific antibodies elicited in this phase 1a trial...
  39. doi Autoantibody detection using indirect immunofluorescence on HEp-2 cells
    Ulrich Sack
    Institute of Clinical Immunology and Transfusion Medicine, Medical Faculty of the University, Leipzig, Germany
    Ann N Y Acad Sci 1173:166-73. 2009
    ..The aim is to improve diagnostic tests and care of patients with autoimmune diseases as a central concern of the European Autoimmunity Standardization Initiative (EASI)...
  40. pmc Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly
    P Grandi
    Biochemie Zentrum Heidelberg BZH, University of Heidelberg, Germany
    Mol Biol Cell 8:2017-38. 1997
    ....
  41. pmc Human autoantibodies to diacyl-phosphatidylethanolamine recognize a specific set of discrete cytoplasmic domains
    C C F C Laurino
    Rheumatology Division, Universidade Federal de Sao Paulo, Escola Paulista de Medicina Unifesp EPM, Rua Botucatu 740, Sao Paulo, SP 04023 062, Brazil
    Clin Exp Immunol 143:572-84. 2006
    ..The clinical association of CDS-1 sera included a variety of systemic and organ-specific autoimmune diseases but they were also observed in patients with no evidence of autoimmune disease...
  42. ncbi Risk factors for infection with malaria in a low endemic community in Bataan, the Philippines
    M A Lansang
    Research Institute for Tropical Medicine, Department of Health, Alabang, Muntinlupa, Metro Manila, Philippines
    Acta Trop 63:257-65. 1997
    ..16, 0.52). The data suggest that in low-endemic communities like Morong, Bataan, control efforts should be primarily directed to focal areas identified by serology, particularly among migrants and among male young adults...
  43. pmc RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery
    Asa E Y Engqvist-Goldstein
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Mol Biol Cell 15:1666-79. 2004
    ..Our results provide evidence that Hip1R helps to make the interaction between actin and the endocytic machinery functional and transient...
  44. pmc Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein
    H J Matthies
    Division of Cellular and Molecular Medicine, University of California at an San Diego 92093 0683, USA
    J Cell Biol 134:455-64. 1996
    ..Based on these observations, we propose that microtubule bundling by the NCD kinesin-like protein promotes assembly of a stable bipolar spindle in the absence of typical MTOCs...
  45. pmc Heterogeneity of a fluorescent tegument component in single pseudorabies virus virions and enveloped axonal assemblies
    T del Rio
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    J Virol 79:3903-19. 2005
    ..These results support an assembly process in which PRV capsids acquire a membrane in the cell body prior to axonal entry and subsequent transport...
  46. pmc Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 10:R131. 2008
    ..The primary goal of this study was to determine the sensitivity of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P antibodies as detected by other immunoassays...
  47. ncbi Spotted fever in Brazil: a seroepidemiological study and description of clinical cases in an endemic area in the state of São Paulo
    E R de Lemos
    Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil
    Am J Trop Med Hyg 65:329-34. 2001
    ..Twenty-two (4.2%) of these samples demonstrated titers > or = 1:64. The results indicate that Brazilian spotted fever is endemic within this region of Brazil...
  48. ncbi Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants
    M S Toledo
    Department of Biochemistry, , Rua Botucatu 862, , SP, 04023-900, Brazil
    Glycobiology 11:105-12. 2001
    ..fumigatus was also observed. The availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed...
  49. pmc A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
    Theophany Eystathioy
    Department of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    Mol Biol Cell 13:1338-51. 2002
    ..We propose that the GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene transcripts involved in cell growth and homeostasis...
  50. ncbi Methimazole-induced antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis and lupus-like syndrome with a cutaneous feature of vesiculo-bullous systemic lupus erythematosus
    Haw Yueh Thong
    Acta Derm Venereol 82:206-8. 2002
  51. pmc G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214
    J van Deursen
    Department of Genetics, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    EMBO J 15:5574-83. 1996
    ..Our data suggest that CAN is critical to cell cycle progression and required for both nuclear protein import and mRNA export...
  52. ncbi Serological investigation of Bartonella henselae infections in clinically cat-scratch disease-suspected patients, patients with cardiovascular diseases, and healthy veterinary students in Japan
    Eisaku Kikuchi
    Department of Veterinary Medicine, College Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan
    Microbiol Immunol 46:313-6. 2002
    ..henselae antibody had had some contacts with cats. In CSD-suspected patients, the B. henselae positive rate in females was significantly higher than in males, and high seropositive rates to B. henselae were found in younger age groups...
  53. ncbi Cadherin-6, a cell adhesion molecule specifically expressed in the proximal renal tubule and renal cell carcinoma
    R Paul
    James Buchanan Brady Urological Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Cancer Res 57:2741-8. 1997
    ..Overall, these findings demonstrate specific expression of cadherin-6 in the proximal renal tubules in normal human kidney and suggest that alterations of cadherin-6 expression are associated with progression of renal cell carcinoma...
  54. ncbi Functional genomics of gam56: characterisation of the role of a 56 kilodalton sexual stage antigen in oocyst wall formation in Eimeria maxima
    Sabina I Belli
    Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Westbourne Street, Gore Hill, NSW 2065, Australia
    Int J Parasitol 32:1727-37. 2002
    ..In addition, these findings provide evidence for the molecular mechanisms underlying oocyst wall formation in Eimeria and the role of gametocyte antigens in this process...
  55. ncbi Transient receptor potential (TRPC) channels in human sperm: expression, cellular localization and involvement in the regulation of flagellar motility
    Laura E Castellano
    Department of Genetics of Development and Molecular Physiology, Institute of Biotechnology, UNAM, Cuernavaca, Mexico
    FEBS Lett 541:69-74. 2003
    ..Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility...
  56. pmc Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p
    J D Aitchison
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021, USA
    J Cell Biol 131:1133-48. 1995
    ....
  57. ncbi The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes
    W G Whitfield
    Department of Biological Sciences, University, Dundee, UK
    J Cell Sci 108:3377-87. 1995
    ..The data suggest that the function of CP190 involves cell cycle dependent associations with both the centrosome, and with specific chromosomal loci...
  58. ncbi Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif
    R Erlich
    Department of Pathology and Immunology, Monash University Medical School, Melbourne, Victoria 3181, Australia
    J Biol Chem 271:8328-37. 1996
    ..This is the first report of a cytosolic Golgi protein containing a granin motif. The structural characteristics of p230 indicate that it may play a role in vesicular transport from the trans-Golgi...
  59. ncbi p38 MAPK signaling during murine preimplantation development
    David R Natale
    Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1
    Dev Biol 268:76-88. 2004
    ..This study demonstrates that p38 MAPK activity is required to support development through the murine preimplantation interval...
  60. ncbi CD36-dependent adhesion and knob expression of the transmission stages of Plasmodium falciparum is stage specific
    K P Day
    Wellcome Trust Centre for Epidemiology of Infectious Disease, Department of Zoology, University of Oxford
    Mol Biochem Parasitol 93:167-77. 1998
    ..The adhesion phenotype of these young gametocytes was indistinguishable from that of the trophozoites by all criteria examined. These data support the hypothesis that other host receptors mediate the binding of late gametocytes...
  61. ncbi alpha-Internexin is the only neuronal intermediate filament expressed in developing cerebellar granule neurons
    C L Chien
    Department of Pathology, Columbia University College of Physicans and Surgeons, New York, 10032, USA
    J Neurobiol 29:304-18. 1996
    ..Furthermore, alpha-internexin may play a key role in neurite outgrowth and the establishment of neuronal cytoarchitecture...
  62. ncbi A comprehensive analysis of the expression of crystallins in mouse retina
    Jinghua Xi
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Vis 9:410-9. 2003
    ..This prompted us to undertake a comprehensive investigation to delineate the baseline expression of crystallin genes in the adult mouse retina...
  63. ncbi Antinuclear antibodies specific for primary biliary cirrhosis
    Howard J Worman
    Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, 10th Floor, Room 508, New York, NY 10032, USA
    Autoimmun Rev 2:211-7. 2003
    ..While antibodies against gp210, sp100 and some other nuclear proteins are very specific to PBC and may therefore be useful diagnostic markers, their connection to pathogenesis remains to be elucidated...
  64. ncbi Profile and clinical significance of anti-nuclear envelope antibodies found in patients with primary biliary cirrhosis: a multicenter study
    Kiyomitsu Miyachi
    First Diagnostic Division, Health Sciences Research Institute Inc, 106 Godo cho Hodogaya ku, Yokohama shi, Kanagawa 240 0005, Japan
    J Autoimmun 20:247-54. 2003
    ..This difference was found to be statistically significant. The presence of anti-p62 complex antibody may be related with the progressive or advanced state of PBC...
  65. ncbi Biochemical characterisation of the 56 and 82 kDa immunodominant gametocyte antigens from Eimeria maxima
    Sabina I Belli
    Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Gore Hill, Westbourne Street, Sydney, N S W 2065, Australia
    Int J Parasitol 32:805-16. 2002
    ..Characterisation of these novel proteins has provided further insights into the molecular mechanisms of gametocyte differentiation in E. maxima...
  66. pmc Detection and identification of antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testing
    I Peene
    Department of Rheumatology, Ghent University Hospital, Belgium
    Ann Rheum Dis 60:1131-6. 2001
    ..CONCLUSIONS: The data from this analysis are useful in estimating the probabilities of detecting specific ANA. Line immunoassay was shown to be a sensitive test for the detection of anti-ENA antibodies...
  67. ncbi Differential nuclear localization of the cancer/testis-associated protein, SPAN-X/CTp11, in transfected cells and in 50% of human spermatozoa
    V A Westbrook
    Departments of Cell Biology and Obstetrics and Gynecology, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    Biol Reprod 64:345-58. 2001
    ....
  68. pmc Collection of corneal impression cytology directly on a sterile glass slide for the detection of viral antigen: an inexpensive and simple technique for the diagnosis of HSV epithelial keratitis - a pilot study
    S Athmanathan
    Jhaveri Microbiology Center, Hyderabad Eye Research Foundation, India
    BMC Ophthalmol 1:3. 2001
    ..Conventional virology techniques are often expensive and time consuming. We describe here a highly economical, simple, rapid and sensitive technique for the collection of impression cytology, for the laboratory diagnosis of HSK...
  69. ncbi PKC isoenzymes in the chicken lens and TPA-induced effects on intercellular communication
    V M Berthoud
    Department of Pediatrics, University of Chicago, Illinois 60637 1470, USA
    Invest Ophthalmol Vis Sci 41:850-8. 2000
    ....
  70. ncbi Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts
    R Tournebize
    Cell Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 2:13-9. 2000
    ..These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1...
  71. ncbi Anti-NuMA antibodies: an uncommon specificity in scleroderma sera
    R Herrera-Esparza
    Department of Immunology, Universidad Autonoma de Zacatecas, Mexico
    Rev Rhum Engl Ed 66:315-8. 1999
    ..Serum antibodies in scleroderma patients are generally directed against the nucleolus and centromeres. A small proportion of patients have serum antibodies to the centrioles and mitotic apparatus...
  72. ncbi Nestin as a marker for proliferative endothelium in gliomas
    Ken ichi Sugawara
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Lab Invest 82:345-51. 2002
    ..As expected, nestin immunostained strongly in all four samples of hemangioblastomas. We suggest that nestin is not only a marker for neuroepithelial stem cells and glioma cells but also for tumor endothelial cells during rapid growth...
  73. ncbi Lipopolysaccharide-dependent induction of leech leukocytes that cross-react with vertebrate cellular differentiation markers
    M de Eguileor
    DBSF University of Insubria, Varese, Italy
    Tissue Cell 32:437-45. 2000
    ..CD61 and CD68 both recognised a band of about 110 kD; CD11b recognised a band of 200 kD, and CD11c, a band of 180 kD...
  74. ncbi Lack of iNOS facilitates MCMV spread in the retina
    Ming Zhang
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, Georgia 30912, USA
    Invest Ophthalmol Vis Sci 48:285-92. 2007
    ..The purposes of this study were to identify iNOS-producing retinal cells and to determine whether lack of iNOS facilitates MCMV spread and replication in the retina...
  75. ncbi A fusion protein of IgG fc and mouse-derived antigen on the surface of pseudorabies virus particles does not accelerate production of harmful auto-reactive antibodies
    Haruko Ota
    Department of Global Agricultural Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    J Vet Med Sci 68:1179-83. 2006
    ..Therefore, this study confirmed that immunization with PRV/Fc did not induce harmful auto-reactive antibodies...
  76. ncbi Emodin suppression of ocular surface inflammatory reaction
    Ai Kitano
    Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan
    Invest Ophthalmol Vis Sci 48:5013-22. 2007
    ..To determine whether a Chinese herbal medicine component, emodin, suppresses inflammatory/fibrogenic reaction in cultured subconjunctival fibroblasts and reduces injury-induced increases in ocular surface inflammation in mice...
  77. ncbi Role of NF-kappaB and MAPKs in light-induced photoreceptor apoptosis
    Li Ping Yang
    Peking University Eye Center, Peking University Third Hospital, Peking University, Beijing, China
    Invest Ophthalmol Vis Sci 48:4766-76. 2007
    ....
  78. pmc Expression, subcellular localization, and regulation of sigma receptor in retinal muller cells
    Guoliang Jiang
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912 2000, USA
    Invest Ophthalmol Vis Sci 47:5576-82. 2006
    ..This study examined the expression, subcellular localization, binding activity, and regulation of sigmaR1 in retinal Müller cells...
  79. ncbi Rapid apoptosis induction by IGFBP-3 involves an insulin-like growth factor-independent nucleomitochondrial translocation of RXRalpha/Nur77
    Kuk Wha Lee
    Division of Pediatric Endocrinology, Mattel Children s Hospital at UCLA, David Geffen School of Medicine, Los Angeles, California 90095, USA
    J Biol Chem 280:16942-8. 2005
    ..This unique nongenotropic nuclear pathway supports an emerging role for IGFBP-3 as a novel, multicompartmental signaling molecule involved in induction of apoptosis in malignant cells...
  80. ncbi PAF-induced furin and MT1-MMP expression is independent of MMP-2 activation in corneal myofibroblasts
    Paulo Ottino
    Department of Ophthalmology and Neuroscience Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
    Invest Ophthalmol Vis Sci 46:487-96. 2005
    ..Corneal stromal myofibroblasts express the platelet-activating factor (PAF) receptor, but its role is unclear. In the present study, the effect of PAF on induction of metalloproteinases (MMPs) was investigated...
  81. ncbi Novel splicing variant of mouse Orc1 is deficient in nuclear translocation and resistant for proteasome-mediated degradation
    Yasuyuki Miyake
    Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
    J Biol Chem 280:12643-52. 2005
    ....
  82. ncbi Entamoeba histolytica: identification of a distinct beta2 integrin-like molecule with a potential role in cellular adherence
    Dylan R Pillai
    Division of Infectious Diseases, Department of Medicine, University of California, San Francisco 94143, USA
    Exp Parasitol 109:135-42. 2005
    ..The presence of integrin-dependent binding may allow trophozoites to opportunistically adhere to activated intestinal epithelium or vascular endothelium expressing ICAM-1 during amebic colitis or hepatic abscess...
  83. ncbi Tissue-type plasminogen activator acts as a cytokine that triggers intracellular signal transduction and induces matrix metalloproteinase-9 gene expression
    Kebin Hu
    Department of Pathology, University of Pittsburgh School of Medicine, S 405 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    J Biol Chem 281:2120-7. 2006
    ....
  84. ncbi Adenovirus type 37 keratitis in the C57BL/6J mouse
    Ashish V Chintakuntlawar
    Molecular Pathogenesis of Eye Infection Research Center, Dean A McGee Eye Institute OUHSC, Oklahoma City, OK 73104, USA
    Invest Ophthalmol Vis Sci 48:781-8. 2007
    ..To develop a mouse model of adenoviral keratitis that will allow further study of viral and host pathogenic mechanisms...
  85. ncbi Tumor necrosis factor-alpha-induced apoptosis in murine cytomegalovirus retinitis
    Jun Zhou
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA
    Invest Ophthalmol Vis Sci 48:1691-700. 2007
    ..To explore the mechanism underlying retinal apoptosis in MCMV retinitis, this study was initiated to determine whether the tumor necrosis factor receptor (TNFR)1-TNF pathway is involved in apoptosis during MCMV retinitis...
  86. ncbi Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri Lanka
    A Naguleswaran
    Institute of Parasitology, University of Bern, Laenggass Str 122, CH 3001 Bern, Switzerland
    Vet Parasitol 126:257-62. 2004
    ..caninum antibodies. The optimal correlation between ELISA, IFAT, and Western blot confirms the suitability of the ELISA for large-scale seroepidemiologic studies, not only in cattle but also in goats...
  87. ncbi RNA interference targeting transforming growth factor-beta type II receptor suppresses ocular inflammation and fibrosis
    Hiroshi Nakamura
    Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, 60612, USA
    Mol Vis 10:703-11. 2004
    ..The purpose of the study was to determine whether small interference RNAs (siRNAs) targeting the type II receptor of TGF-beta (TbetaRII) could be used to suppress the TGF-beta action...
  88. ncbi PLP2/A4 interacts with CCR1 and stimulates migration of CCR1-expressing HOS cells
    Sang Min Lee
    Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin, Kyunggi do 449 701, Republic of Korea
    Biochem Biophys Res Commun 324:768-72. 2004
    ..Overexpression of PLP2/A4 stimulated a twofold increase in the agonist-induced migration of HOS/CCR1 cells, implicating a functional role for PLP2/A4 in the chemotactic processes via CCR1...
  89. ncbi Icariin-mediated modulation of cell cycle and p53 during cardiomyocyte differentiation in embryonic stem cells
    Danyan Zhu
    Department of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China
    Eur J Pharmacol 514:99-110. 2005
    ..Results showed p53 to be an important regulator in the differentiation in embryonic stem cells treated with 10(-7) M icariin, controlling or adjusting the balance between differentiated cells and cells undergoing apoptosis...
  90. ncbi Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors
    Gabriela Kania
    Institute of Plant Genetics and Crop Plant Research IPK, Corrensstr 3, D 06466 Gatersleben, Germany
    Stem Cells 23:791-804. 2005
    ..In conclusion, we present the somatic stem cell marker prominin-1 as a new parameter to define ES-derived committed and early progenitor cells...
  91. pmc Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract
    Hua He
    TissueTech, Inc and the Ocular Surface Center, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 49:4468-75. 2008
    ..Experimentally, the authors and others have reported that AM facilitates macrophage apoptosis. However, it remains unclear whether such anti-inflammatory activity is retained in AM extract (AME)...
  92. ncbi LBP and CD14 secreted in tears by the lacrimal glands modulate the LPS response of corneal epithelial cells
    David R Blais
    Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ontario, Canada
    Invest Ophthalmol Vis Sci 46:4235-44. 2005
    ..Better understanding of the spatial expression of the LPS receptor components at the tear-corneal interface might facilitate enhanced functions of the LPS receptor complex in ocular defense against Gram-negative infections...
  93. ncbi Plasmodium yoelii: axenic development of the parasite mosquito stages
    Johanna M Porter-Kelley
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Exp Parasitol 112:99-108. 2006
    ..Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations...
  94. ncbi [Evaluation of the IgG anti-Toxoplasma response and its avidity by western-blot in HIV-patients]
    María Cristina Sarmiento
    Instituto de Salud en el Tropico, Facultad de Medicina Universidad Nacional de Colombia, Bogota, Colombia
    Rev Cubana Med Trop 57:125-32. 2005
    ..The intensity of the bands measured by densitometry varied significantly for proteins of 66 and 31 kDa. According to the results, these proteins are of interest to evaluate their role in the reactivation of toxoplasmosis in HIV patients...
  95. ncbi Impaired responses of leukemic dendritic cells derived from a human myeloid cell line to LPS stimulation
    Kwang Dong Kim
    Department of Biological Sciences, Sookmyung Women s University, Seoul 140 742, Korea
    Exp Mol Med 38:72-84. 2006
    ..Taken together, our findings suggest that, although MuDC express TLR4, unlike TNF-alpha and IL-1beta, LPS does not stimulate MuDC to acquire mature phenotypes, and they may have impaired activity to initiate innate immune response...
  96. ncbi TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression
    Susan G Elner
    Department of Ophthalmology, University of Michigan, W K Kellogg Eye Center, Ann Arbor 48105, USA
    Invest Ophthalmol Vis Sci 46:4627-33. 2005
    ..This study was performed to demonstrate the classical role of TLR4 in HRPE endotoxin (lipopolysaccharide; LPS) binding leading to HRPE proinflammatory cytokine secretion...
  97. pmc The complement system plays a critical role in the development of experimental autoimmune anterior uveitis
    Purushottam Jha
    Department of Ophthalmology, Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Invest Ophthalmol Vis Sci 47:1030-8. 2006
    ..The role of complement in ocular autoimmunity was explored in a experimental autoimmune anterior uveitis (EAAU) animal model...
  98. ncbi CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes
    Sebastien Vankoningsloo
    Laboratory of Biochemistry and Cellular Biology, University of Namur F U N D P, rue de Bruxelles, 61, 5000 Namur, Belgium
    J Cell Sci 119:1266-82. 2006
    ..These results highlight a new role for CREB in the control of triglyceride metabolism during the adaptative response of preadipocytes to mitochondrial dysfunction...
  99. ncbi Noncanonical cAMP pathway and p38 MAPK mediate beta2-adrenergic receptor-induced IL-6 production in neonatal mouse cardiac fibroblasts
    Feng Yin
    Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100083, China
    J Mol Cell Cardiol 40:384-93. 2006
    ..The present results suggest that beta(2)-AR mediates IL-6 production through a noncanonical cAMP responsible pathway and p38 MAPK...
  100. ncbi Hypoxic damage to the periventricular white matter in neonatal brain: role of vascular endothelial growth factor, nitric oxide and excitotoxicity
    Charanjit Kaur
    Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    J Neurochem 98:1200-16. 2006
    ..Our findings point towards the involvement of excitotoxicity, VEGF and NO in periventricular white matter damage in response to hypoxia...
  101. ncbi Autoimmune subepidermal blistering diseases in Uganda: correlation of autoantibody class with age of patients
    Grace K Mulyowa
    Department of Dermatology, University of Wurzburg, Wurzburg, Germany
    Int J Dermatol 45:1047-52. 2006
    ..No data are available on the incidence and immunoreactivity of autoimmune subepidermal blistering skin diseases in East Africa...

Research Grants70

  1. P2Y2 Purinergic Receptor and Membrane Microdomains
    Fernando Gonzalez; Fiscal Year: 2004
    ..abstract_text> ..
  2. REGULATION OF DENDRITIC CELL-LYMPHOCYTE INTERACTIONS
    Clare Twist; Fiscal Year: 1993
    ....
  3. LYME DISEASE IN WESTERN USA--ECOLOGY AND EPIDEMIOLOGY
    Robert Lane; Fiscal Year: 1991
    ..The effects of exposure to direct sunlight or of spirochetal infection on questing by immature ticks also will be determined...
  4. LYME DISEASE IN WESTERN USA--ECOLOGY AND EPIDEMIOLOGY
    Robert Lane; Fiscal Year: 1990
    ..The effects of exposure to direct sunlight or of spirochetal infection on questing by immature ticks also will be determined...
  5. LYME DISEASE IN WESTERN USA--ECOLOGY AND EPIDEMIOLOGY
    Robert Lane; Fiscal Year: 1992
    ..The effects of exposure to direct sunlight or of spirochetal infection on questing by immature ticks also will be determined...
  6. HSV1 PROCESSING/PACKAGING GENES
    Sandra Weller; Fiscal Year: 1999
    ..It is anticipated that an analysis of the processing/packaging proteins will not only provide an understanding of the mechanisms of their action but may also lead to the development of novel strategies for antiviral therapy. ..
  7. HSV-1 PROCESSING AND PACKAGING GENES
    Sandra Weller; Fiscal Year: 1999
    ..It is anticipated that an analysis of the processing/packaging proteins will not only provide an understanding of the mechanisms of their action but may also lead to the development of novel strategies for antiviral therapy. ..
  8. EUBACTERIA IN PATHOGENESIS OF SYSTEMIC LUPUS ERYTHEMATOSUS
    SHIRLEY KOVACS; Fiscal Year: 2000
    ..The possibility that bacteria are etiological agents of SLE unveils a wealth of antibiotic therapeutic opportunities for treatment of SLE or other autoimmune, rheumatological disorders. ..
  9. PATHOGENESIS OF MEMBRANOUS GLOMERULONEPHROPATHY
    Sudesh Makker; Fiscal Year: 1990
    ..This knowledge will lead to further progress in the understanding of the mechanism of MGN...
  10. CUTANEOUS LUPUS ERYTHEMATOSUS PATHOGENESIS MECHANISMS
    JAMES GILLIAM; Fiscal Year: 1980
    ..These studies will provide insight into the relative roles of cellular versus humoral mechanisms in the pathogenesis of LE skin disease amd many lead to a more rational approach to treatment...
  11. GRANULOCYTE CYTOPLASMIC ANTIGENS IN RENAL VASCULITIS
    M Amin Arnaout; Fiscal Year: 1990
    ..Structural characterization of these antigens may also make it possible to generate specific reagents that will improve the diagnostic potential of currently available methods of detecting the autoantibodies...
  12. GRANULOCYTE CYTOPLASMIC ANTIGENS IN RENAL VASCULITIS
    M Amin Arnaout; Fiscal Year: 1993
    ..Structural characterization of these antigens may also make it possible to generate specific reagents that will improve the diagnostic potential of currently available methods of detecting the autoantibodies...
  13. PROTEIN SORTING TO THE LYSOSOME-LIKE VACUOLE IN YEAST
    Scott Emr; Fiscal Year: 1992
    ..It is anticipated that these studies will result in a detailed view of the organization and control of protein sorting and vesicular traffic between the Golgi complex and the vacuole/lysosome in yeast and other eukaryotes...
  14. BOVINE LEUKEMIA VIRUS
    DON GRAVES; Fiscal Year: 1980
    ..It is also possible that there exists more than one strain of BLV and these studies may give suggestive evidence of this...
  15. CAVEOLAE AND CAVEOLINS IN C6 GLIAL CELL DIFFERENTIATION
    Walter Silva; Fiscal Year: 2001
    ..Using this integrated approach this aim promises to shed light on the relevance of caveolins and caveolae to the cell signaling and signal transduction processes of glial cells. ..
  16. CAVEOLAE AND CAVEOLINS IN C6 GLIAL CELL DIFFERENTIATION
    Walter Silva; Fiscal Year: 2000
    ..Using this integrated approach this aim promises to shed light on the relevance of caveolins and caveolae to the cell signaling and signal transduction processes of glial cells. ..
  17. PLEURAL DOSIMETRY AND BIOMARKERS OF RESPONSE TO FIBERS
    Agnes Kane; Fiscal Year: 1992
    ..Ultimately, this new model could be used to extrapolate dose-response relationships to human exposure to asbestos and man-made mineral fibers in order to assess more accurately the risk of developing malignant mesotheliomas...
  18. SERTOLI CELL-GERM CELL SURFACE MEMBRANE INTERACTIONS
    ROBERT DEPHILIP; Fiscal Year: 1990
    ..This information should find immediate application in the consideration of spermatogenic dysfunction and in the design of protocols able to safely regulate male germ cell development...
  19. STEROID REGULATION OF CONNEXIN43 IN MYOMETRIUM
    Janet Andersen; Fiscal Year: 1992
    ....
  20. NEUTROPHIL AUTOANTIBODIES IN VASCULITIS AND NEPHRITIS
    Ronald Falk; Fiscal Year: 1990
    ..The discovery that ANCA cause granulocytes to release reactive oxygen species and proteolytic enzymes will provide novel insights into the pathogenesis of arteritis...
  21. PATHOGENESIS OF PAGET'S DISEASE OF BONE
    MICHAEL VIOLA; Fiscal Year: 1980
    ....
  22. COPPER HOMEOSTASIS IN YEAST
    Dennis Thiele; Fiscal Year: 1999
    ..The experiments proposed in this application are aimed at elucidating the fundamental processes by which yeast, and all organisms, establish and maintain normal Cu homeostasis. ..
  23. STRUCTURE, FUNCTION, AND ASSEMBLY OF DESHEMI DESMOSOMES
    Jonathan Jones; Fiscal Year: 1990
    ..These studies may support a hypothesis that these two organelles play a role in mediating the kinds of cell interactions which are essential for tissue morphogenesis and the maintenance of tissue integrity...
  24. FAA AND FAD GENES AND PROTEINS
    Manuel Buchwald; Fiscal Year: 1999
    ..The fifth aim is to isolate the FAE gene by the expression cloning used so successfully to date. ..
  25. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2006
    ..The involvement of GABAA receptors in anxiety, sleep and seizure disorders underscores the importance of understanding the cellular and molecular mechanisms of this receptor. ..
  26. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2003
    ..The involvement of GABAA receptors in anxiety, sleep and seizure disorders underscores the importance of understanding the cellular and molecular mechanisms of this receptor. ..
  27. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2005
    ..The involvement of GABAA receptors in anxiety, sleep and seizure disorders underscores the importance of understanding the cellular and molecular mechanisms of this receptor. ..
  28. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2002
    ..The involvement of GABAA receptors in anxiety, sleep and seizure disorders underscores the importance of understanding the cellular and molecular mechanisms of this receptor. ..
  29. Regulation of GABAA Receptor Cell Surface Expression
    NANCY LEIDENHEIMER; Fiscal Year: 2004
    ..The involvement of GABAA receptors in anxiety, sleep and seizure disorders underscores the importance of understanding the cellular and molecular mechanisms of this receptor. ..
  30. A GENE FAMILY EXPRESSED IN SEA URCHIN EMBRYONIC ECTODERM
    WILLIAM KLEIN; Fiscal Year: 1992
    ..Antibodies against the Spec3 protein will be generated from the Spec3 ORF and the location of the protein in urchin embryos will be determined by immunological detection...
  31. A GENE FAMILY EXPRESSED IN SEA URCHIN EMBRYONIC ECTODERM
    WILLIAM KLEIN; Fiscal Year: 1991
    ..Antibodies against the Spec3 protein will be generated from the Spec3 ORF and the location of the protein in urchin embryos will be determined by immunological detection...
  32. A GENE FAMILY EXPRESSED IN SEA URCHIN EMBRYONIC ECTODERM
    WILLIAM KLEIN; Fiscal Year: 1990
    ..Antibodies against the Spec3 protein will be generated from the Spec3 ORF and the location of the protein in urchin embryos will be determined by immunological detection...
  33. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 1991
    ..I expect to continue in the future collaborations that have already been established so that my research program can continually take advantage of technologies developed by current and future collaborators...
  34. UBIQUITIN AND THE CYTOSKELETON IN ALZHEIMER DISEASE
    VICTOR FRIED; Fiscal Year: 1992
    ..The identification of specific ubiquitinated components in AD may provide new rationales for developing probes for diagnosis and therapeutic strategies...
  35. FUNCTIONS OF ALTERNATIVELY SPLICED CARTILAGE FIBRONECTIN
    Vickie Bennett; Fiscal Year: 1991
    ..These studies will provide important information regarding the role of fibronectin in the cartilage matrix and during cartilage differentiation...
  36. BASOPHIL ACTIVATION AND RECRUITMENT IN ALLGERGIC DISEASE
    Bruce Bochner; Fiscal Year: 1990
    ....
  37. Immunofluorescence Standards for Flow Cytometry
    Douglas Redelman; Fiscal Year: 2003
    ..abstract_text> ..
  38. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 1993
    ..Through these studies we hope to gain a better understanding of how regulated modification of a signal transducing protein influences its subcellular compartmentalization and association with ancillary factors that modify its function...
  39. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 1992
    ..Through these studies we hope to gain a better understanding of how regulated modification of a signal transducing protein influences its subcellular compartmentalization and association with ancillary factors that modify its function...
  40. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    Donald Defranco; Fiscal Year: 1991
    ..Through these studies we hope to gain a better understanding of how regulated modification of a signal transducing protein influences its subcellular compartmentalization and association with ancillary factors that modify its function...
  41. LYME ARTHRITIS: A NEW EPIDEMIC DISEASE
    Allen Steere; Fiscal Year: 1980
    ..If the agent can be identified, a serologic test for diagnosis will be developed, and attack rates will be determined within sample human and animal populations. The results would provide the basis for decisions about control measures...
  42. Rapid point of care HIV PCR diagnostic device
    John Gerdes; Fiscal Year: 2009
    ..We propose the miniaturization and automation of nucleic acid testing methods to enable low cost, near patient testing. ..
  43. EXPRESSION OF RETROVIRAL ENVELOPE GENE FUSION PROTEINS
    Daniel Donoghue; Fiscal Year: 1990
    ..This will allow us to examine the oncogenic potential of these related genes...
  44. INTERACTION OF ORAL BACTERIA WITH PERIODONTAL TISSUES
    Jorgen Slots; Fiscal Year: 1990
    ..The findings may lead to the formulation of new and more effective means of diagnosis, prevention and treatment of human periodontitis...
  45. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1991
    ..and role of these proteins in nucleating microtubule assembly will be examined.' The long term goal of these studies is to understand at a molecular level the components and events necessary for successful mitosis and cell growth...
  46. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1993
    ..and role of these proteins in nucleating microtubule assembly will be examined.' The long term goal of these studies is to understand at a molecular level the components and events necessary for successful mitosis and cell growth...
  47. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1992
    ..and role of these proteins in nucleating microtubule assembly will be examined.' The long term goal of these studies is to understand at a molecular level the components and events necessary for successful mitosis and cell growth...
  48. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Michael Snyder; Fiscal Year: 1990
    ..and role of these proteins in nucleating microtubule assembly will be examined.' The long term goal of these studies is to understand at a molecular level the components and events necessary for successful mitosis and cell growth...
  49. MONOCLONAL ANTIBODIES TO PROSTATE TUMOR ANTIGENS
    George Wright; Fiscal Year: 1991
    ..It is expected the experiments outlined in this grant proposal will yield a panel of well-characterized Mabs that will have clinical applicability...
  50. STUDY OF RABIES AND RABIES VIRUS ANTIGENS
    Hilary Koprowski; Fiscal Year: 1980
    ....
  51. BIOLOGICAL MONITORING FOR EXPOSURE TO COAL TAR
    Regina Santella; Fiscal Year: 1990
    ..Levels of the different biological markers will be correlated with exposure. These studies will thus develop and validate methods to monitor the biological effects of human exposure to PAHs and serve as a model system for skin exposure...
  52. MOLECULAR CYTOGENETICS OF HETEROCHROMATIN
    JOEL EISSENBERG; Fiscal Year: 1990
    ....