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Genomes and Genes | indirect fluorescent antibody techniqueSummarySummary: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984) Top Publications
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Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane culturesJayanta Debnath
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Methods 30:256-68. 2003....- Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plantsM S Toledo
Department of Biochemistry, , Rua Botucatu 862, , SP, 04023-900, Brazil
Glycobiology 11:105-12. 2001..fumigatus was also observed. The availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed... - A serum autoantibody marker of neuromyelitis optica: distinction from multiple sclerosisVanda A Lennon
Department of Neurology, Mayo Clinic Rochester, Rochester, MN 55905, USA
Lancet 364:2106-12. 2004..We assessed the capacity of a putative marker for neuromyelitis optica (NMO-IgG) to distinguish neuromyelitis optica and related disorders from multiple sclerosis... - Clinical applications of antineutrophil cytoplasmic antibody testingWilhelm H Schmitt
Vth Medical Clinic Nephrology, Endocrinolgy, Rheumatolgy, University Clinic Mannheim, Faculty of Clinical Medicine of the University of Heidelberg, Mannheim, Germany
Curr Opin Rheumatol 16:9-17. 2004..This review summarizes the most recent developments in the field, identifies areas of uncertainty, and gives practical guidelines... - Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusionStuart D Blacksell
Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand
Clin Infect Dis 44:391-401. 2007..We suggest that the diagnosis of scrub typhus using IFA should be based on a > or =4-fold increase in the titer in paired serum samples and should only be based on a single sample titer when there is an adequate local evidence base... 
SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeletonFarhana Haque
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
Mol Cell Biol 26:3738-51. 2006..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...- Risk factors for infection with malaria in a low endemic community in Bataan, the PhilippinesM A Lansang
Research Institute for Tropical Medicine, Department of Health, Alabang, Muntinlupa, Metro Manila, Philippines
Acta Trop 63:257-65. 1997..16, 0.52). The data suggest that in low-endemic communities like Morong, Bataan, control efforts should be primarily directed to focal areas identified by serology, particularly among migrants and among male young adults... - In situ identification of mycobacteria in Crohn's disease patient tissue using confocal scanning laser microscopyS A Naser
Department of Molecular Biology and Microbiology, University of Central Florida, Orlando 32816, USA
Mol Cell Probes 16:41-8. 2002..This technique adds an additional approach to investigate MAP role in CD etiology especially when the culture approach is long and inconsistent... - Evaluation of the BioPlex 2200 ANA screen: analysis of 510 healthy subjects: incidence of natural/predictive autoantibodiesO Shovman
Department of Medicine B and Center for Autoimmune Diseases, Chaim Sheba Medical Center, Tel-Hashomer 52621, Israel
Ann N Y Acad Sci 1050:380-8. 2005..252) to the ELISA ANA screening test. Like the ELISA, the BioPlex 2200 has a lower (P < 0.001) positive rate than IIF for the autoantibody screening... - The in vitro and in vivo proliferative capacity of serum-free cultivated human conjunctival epithelial cellsLeonard P K Ang
Singapore National Eye Center, Singapore
Curr Eye Res 28:307-17. 2004..To investigate the in vitro and in vivo proliferative capacity of human conjunctival epithelial cells cultured in serum-free media, and to compare this with current methods that utilize serum-containing media and 3T3 feeder layers... - [Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing]Xiao Ran Yang
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhonghua Liu Xing Bing Xue Za Zhi 28:688-91. 2007..To detect Bartonella henselae IgG antibody among healthy people in Changping, Beijing... - Synapsin and synaptic vesicle protein expression during embryonic and post-natal lens fiber cell differentiationPeter H Frederikse
Department of Pharmacology and Physiology, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
Mol Vis 10:794-804. 2004..In the present study we examine lens expression and regulation of a complement of genes associated with cargo and synaptic vesicle transport in neurons... - A virosomal malaria peptide vaccine elicits a long-lasting sporozoite-inhibitory antibody response in a phase 1a clinical trialShinji L Okitsu
Molecular Immunology, Swiss Tropical Institute, Basel, Switzerland
PLoS ONE 2:e1278. 2007..The main objective of this study was to perform a detailed immunological and functional analysis of the CSP-specific antibodies elicited in this phase 1a trial... - p38 mitogen-activated protein kinase (MAPK) first regulates filamentous actin at the 8-16-cell stage during preimplantation developmentAndrew J M Paliga
Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1
Biol Cell 97:629-40. 2005..In the present study, we have investigated the stage-specific action and role of p38 MAPK in regulating filamentous actin during murine preimplantation development... - A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic specklesTheophany Eystathioy
Department of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
Mol Biol Cell 13:1338-51. 2002..We propose that the GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene transcripts involved in cell growth and homeostasis... - Heterogeneity of a fluorescent tegument component in single pseudorabies virus virions and enveloped axonal assembliesT del Rio
Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA
J Virol 79:3903-19. 2005..These results support an assembly process in which PRV capsids acquire a membrane in the cell body prior to axonal entry and subsequent transport... - Prevalence and clinical significance of isotype specific antinuclear antibodies in primary biliary cirrhosisE I Rigopoulou
Institute of Liver Studies, King's College Hospital, Denmark Hill, London SE5 9RS, UK
Gut 54:528-32. 2005..PBC specific ANA, in particular of the IgG3 isotype, are associated with a more severe disease course, possibly reflecting the peculiar ability of this isotype to engage mediators of damage... - Indirect immunofluorescence in autoimmune diseases: assessment of digital images for diagnostic purposeAmelia Rigon
Department of Clinical Medicine, Immunology, and Rheumatology, University Campus Bio Medico, Rome, Italy
Cytometry B Clin Cytom 72:472-7. 2007..To pursue a high image quality without artefacts and reduce interobserver variability, this study aims at evaluating the reliability of automatically acquired digital images of IIF slides for diagnostic purposes... - Mitogen-activated protein kinase (MAPK) blockade of bovine preimplantation embryogenesis requires inhibition of both p38 and extracellular signal-regulated kinase (ERK) pathwaysPavneesh Madan
Department of Physiology and Pharmacology, University of Western Ontario, Child Health Research Institute, 5th Floor Victoria Research Laboratories, 800 Commissioners Road East, London, Ontario, Canada, N6A 4G5
Reproduction 130:41-51. 2005..We have determined that, in variance to what was observed during murine preimplantation development, bovine early embryos progress at normal frequencies to the blastocyst stage in the presence of p38 MAPK inhibitors... - Anti-aquaporin-4 antibody is involved in the pathogenesis of NMO: a study on antibody titreToshiyuki Takahashi
Department of Neurology, Tohoku University Graduate School of Medicine, 1 1 Seiryomachi, Aobaku, Sendai 980 8574, Japan
Brain 130:1235-43. 2007.... - Ge-1 is a central component of the mammalian cytoplasmic mRNA processing bodyJiang Hong Yu
Department of Medicine, Havard Medical School, the Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Charlestown, MA 02129, USA
RNA 11:1795-802. 2005..Taken together, the results of this study show that Ge-1 is a central component of P-bodies and suggest that Ge-1 may act prior to the 5(')-decapping step in mRNA degradation... - Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activationBarry Fong
Department of Physiology and Pharmacology, The University of Western Ontario London, Ontario, Canada
BMC Dev Biol 7:2. 2007.... 
Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cellsRico Hiemann
Department of Biology, Chemistry and Process Technology, Lausitz University of Applied Sciences, Senftenberg, Germany
Autoimmun Rev 9:17-22. 2009..More sophisticated pattern recognition algorithms and novel calibration systems will improve standardised quantifications of IIF image interpretation...- Hominoid-specific SPANXA/D genes demonstrate differential expression in individuals and protein localization to a distinct nuclear envelope domain during spermatid morphogenesisV A Westbrook
Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, VA 22908, USA
Mol Hum Reprod 12:703-16. 2006.... - RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machineryAsa E Y Engqvist-Goldstein
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
Mol Biol Cell 15:1666-79. 2004..Our results provide evidence that Hip1R helps to make the interaction between actin and the endocytic machinery functional and transient... - [Comparison of techniques used to screen blood donations for malaria antibodies]C P Soler
Service de qualification biologique du don, Centre de Transfusion Sanguine des Armées, 1 Rue du Lieutenant Batany, BP 140, 92141 Clamart, France
Med Trop (Mars) 63:587-9. 2003..The exception is malaria antibody detection that is performed by the indirect fluorescent antibody technique using commercially available kits... - The nuclear pore complex protein Tpr is a common autoantigen in sera that demonstrate nuclear envelope staining by indirect immunofluorescenceY Ou
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Calgary, Calgary, AB, Canada
Clin Exp Immunol 136:379-87. 2004..This report indicates that Tpr, a component of the NuPC, is a common target of human autoantibodies that react with the NE... - Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodiesMichael Mahler
Dr Fooke Laboratorien GmbH, Neuss, Germany
Arthritis Res Ther 10:R131. 2008..The primary goal of this study was to determine the sensitivity of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P antibodies as detected by other immunoassays... - Propylthiouracil and carbimazole associated-antineutrophil cytoplasmic antibodies (ANCA) in patients with Graves' diseaseL Harper
Division of Medical Sciences, University of Birmingham, Edgbaston, Birmingham, UK
Clin Endocrinol (Oxf) 60:671-5. 2004..We aimed to investigate whether carbimazole therapy was also associated with increased risk of ANCA... - Autoantibody detection using indirect immunofluorescence on HEp-2 cellsUlrich Sack
Institute of Clinical Immunology and Transfusion Medicine, Medical Faculty of the University, Leipzig, Germany
Ann N Y Acad Sci 1173:166-73. 2009..The aim is to improve diagnostic tests and care of patients with autoimmune diseases as a central concern of the European Autoimmunity Standardization Initiative (EASI)... - Detection of anti-SSA antibodies by indirect immunofluorescenceXavier Bossuyt
Laboratory Medicine and Internal Medicine, University Hospital Leuven, Leuven, Belgium
Clin Chem 50:2361-9. 2004..We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA... - The clinical significance of antinucleolar antibodiesS Khan
Path Links Immunology, Scunthorpe General Hospital, Scunthorpe, UK
J Clin Pathol 61:283-6. 2008..The importance of antinucleolar antibodies seen by indirect immunofluorescence on HEp-2 cells, although associated with systemic sclerosis (SSc), in unselected patients is unknown... - Aquaporin-4 antibodies in neuromyelitis optica and longitudinally extensive transverse myelitisPatrick Waters
Neurosciences Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England
Arch Neurol 65:913-9. 2008..There is increasing recognition of antibody-mediated immunotherapy-responsive neurologic diseases and a need for appropriate immunoassays... - Serum albumin level correlates with disease severity in patients with Hemorrhagic Fever with Renal SyndromeYoung Ok Kim
Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea
J Korean Med Sci 18:696-700. 2003..001), serum creatinine (r=-0.394, p<0.001), and AST (r=-0.251, p=0.002). Our data suggest that hypoalbuminemia frequently occurs in the acute stage of HFRS, and level of serum albumin is associated with the disease severity of HFRS... - p38 MAPK signaling during murine preimplantation developmentDavid R Natale
Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1
Dev Biol 268:76-88. 2004..This study demonstrates that p38 MAPK activity is required to support development through the murine preimplantation interval... - Dynamic reprogramming of DNA methylation in the early mouse embryoFatima Santos
Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
Dev Biol 241:172-82. 2002..The three phases of methylation reprogramming may have roles in imprinting, the control of gene expression, and the establishment of nuclear totipotency... - Antinuclear antibodies specific for primary biliary cirrhosisHoward J Worman
Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, 10th Floor, Room 508, New York, NY 10032, USA
Autoimmun Rev 2:211-7. 2003..While antibodies against gp210, sp100 and some other nuclear proteins are very specific to PBC and may therefore be useful diagnostic markers, their connection to pathogenesis remains to be elucidated... - A comprehensive analysis of the expression of crystallins in mouse retinaJinghua Xi
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA
Mol Vis 9:410-9. 2003..We suggest that crystallins play vital functions in protecting retinal neurons from damage by environmental and/or metabolic stress... - Profile and clinical significance of anti-nuclear envelope antibodies found in patients with primary biliary cirrhosis: a multicenter studyKiyomitsu Miyachi
First Diagnostic Division, Health Sciences Research Institute Inc, 106 Godo cho Hodogaya ku, Yokohama shi, Kanagawa 240 0005, Japan
J Autoimmun 20:247-54. 2003..This difference was found to be statistically significant. The presence of anti-p62 complex antibody may be related with the progressive or advanced state of PBC... - Functional genomics of gam56: characterisation of the role of a 56 kilodalton sexual stage antigen in oocyst wall formation in Eimeria maximaSabina I Belli
Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Westbourne Street, Gore Hill, NSW 2065, Australia
Int J Parasitol 32:1727-37. 2002..In addition, these findings provide evidence for the molecular mechanisms underlying oocyst wall formation in Eimeria and the role of gametocyte antigens in this process... - Nestin as a marker for proliferative endothelium in gliomasKen-ichi Sugawara
Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan
Lab Invest 82:345-51. 2002..As expected, nestin immunostained strongly in all four samples of hemangioblastomas. We suggest that nestin is not only a marker for neuroepithelial stem cells and glioma cells but also for tumor endothelial cells during rapid growth... - Biochemical characterisation of the 56 and 82 kDa immunodominant gametocyte antigens from Eimeria maximaSabina I Belli
Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, Gore Hill, Westbourne Street, Sydney, N S W 2065, Australia
Int J Parasitol 32:805-16. 2002..Characterisation of these novel proteins has provided further insights into the molecular mechanisms of gametocyte differentiation in E. maxima... - Serological investigation of Bartonella henselae infections in clinically cat-scratch disease-suspected patients, patients with cardiovascular diseases, and healthy veterinary students in JapanEisaku Kikuchi
Department of Veterinary Medicine, College Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan
Microbiol Immunol 46:313-6. 2002..henselae antibody had had some contacts with cats. In CSD-suspected patients, the B. henselae positive rate in females was significantly higher than in males, and high seropositive rates to B. henselae were found in younger age groups... - Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteinsPavneesh Madan
Department of Obstetrics and Gynaecology, University of Western Ontario, Children s Health Research Institute Victoria Research Laboratories, London, Ontario N6A 4G5, Canada
J Biol Chem 282:12127-34. 2007.... - Methimazole-induced antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis and lupus-like syndrome with a cutaneous feature of vesiculo-bullous systemic lupus erythematosusHaw-Yueh Thong
Acta Derm Venereol 82:206-8. 2002 - Transient receptor potential (TRPC) channels in human sperm: expression, cellular localization and involvement in the regulation of flagellar motilityLaura E Castellano
Department of Genetics of Development and Molecular Physiology, Institute of Biotechnology, UNAM, Cuernavaca, Mexico
FEBS Lett 541:69-74. 2003..Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility... - Collection of corneal impression cytology directly on a sterile glass slide for the detection of viral antigen: an inexpensive and simple technique for the diagnosis of HSV epithelial keratitis - a pilot studyS Athmanathan
Jhaveri Microbiology Center, Hyderabad Eye Research Foundation, India
BMC Ophthalmol 1:3. 2001..Conventional virology techniques are often expensive and time consuming. We describe here a highly economical, simple, rapid and sensitive technique for the collection of impression cytology, for the laboratory diagnosis of HSK... - alpha-Internexin is the only neuronal intermediate filament expressed in developing cerebellar granule neuronsC L Chien
Department of Pathology, Columbia University College of Physicans and Surgeons, New York, 10032, USA
J Neurobiol 29:304-18. 1996..Furthermore, alpha-internexin may play a key role in neurite outgrowth and the establishment of neuronal cytoarchitecture... - PKC isoenzymes in the chicken lens and TPA-induced effects on intercellular communicationV M Berthoud
Department of Pediatrics, University of Chicago, Illinois 60637 1470, USA
Invest Ophthalmol Vis Sci 41:850-8. 2000.... - Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extractsR Tournebize
Cell Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Nat Cell Biol 2:13-9. 2000..These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1... - Cadherin-6, a cell adhesion molecule specifically expressed in the proximal renal tubule and renal cell carcinomaR Paul
James Buchanan Brady Urological Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
Cancer Res 57:2741-8. 1997..Overall, these findings demonstrate specific expression of cadherin-6 in the proximal renal tubules in normal human kidney and suggest that alterations of cadherin-6 expression are associated with progression of renal cell carcinoma... - Anti-NuMA antibodies: an uncommon specificity in scleroderma seraR Herrera-Esparza
Department of Immunology, Universidad Autonoma de Zacatecas, Mexico
Rev Rhum Engl Ed 66:315-8. 1999..Serum antibodies in scleroderma patients are generally directed against the nucleolus and centromeres. A small proportion of patients have serum antibodies to the centrioles and mitotic apparatus... - Differential nuclear localization of the cancer/testis-associated protein, SPAN-X/CTp11, in transfected cells and in 50% of human spermatozoaV A Westbrook
Departments of Cell Biology and Obstetrics and Gynecology, University of Virginia Health System, Charlottesville, Virginia 22908, USA
Biol Reprod 64:345-58. 2001.... - CD36-dependent adhesion and knob expression of the transmission stages of Plasmodium falciparum is stage specificK P Day
Wellcome Trust Centre for Epidemiology of Infectious Disease, Department of Zoology, University of Oxford
Mol Biochem Parasitol 93:167-77. 1998..The adhesion phenotype of these young gametocytes was indistinguishable from that of the trophozoites by all criteria examined. These data support the hypothesis that other host receptors mediate the binding of late gametocytes... - The development of a serum-free derived bioengineered conjunctival epithelial equivalent using an ultrathin poly(epsilon-caprolactone) membrane substrateLeonard P K Ang
Singapore National Eye Center, Singapore
Invest Ophthalmol Vis Sci 47:105-12. 2006..To evaluate the use of an ultrathin poly(epsilon-caprolactone) (PCL) membrane as a substrate for the development of a serum-free-derived conjunctival epithelial equivalent... - G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214J van Deursen
Department of Genetics, St Jude Children s Research Hospital, Memphis, TN 38105, USA
EMBO J 15:5574-83. 1996..Our data suggest that CAN is critical to cell cycle progression and required for both nuclear protein import and mRNA export... - The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomesW G Whitfield
Department of Biological Sciences, University, Dundee, UK
J Cell Sci 108:3377-87. 1995..The data suggest that the function of CP190 involves cell cycle dependent associations with both the centrosome, and with specific chromosomal loci... - Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152pJ D Aitchison
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021, USA
J Cell Biol 131:1133-48. 1995.... - A novel nuclear structure containing the survival of motor neurons proteinQ Liu
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
EMBO J 15:3555-65. 1996..These structures are similar in number (2-6) and size (0.1-1.0 micron) to coiled bodies, and frequently are found near or associated with coiled bodies. We term these prominent nuclear structures gems, for Gemini of coiled bodies... - Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motifR Erlich
Department of Pathology and Immunology, Monash University Medical School, Melbourne, Victoria 3181, Australia
J Biol Chem 271:8328-37. 1996..This is the first report of a cytosolic Golgi protein containing a granin motif. The structural characteristics of p230 indicate that it may play a role in vesicular transport from the trans-Golgi... - Lipopolysaccharide-dependent induction of leech leukocytes that cross-react with vertebrate cellular differentiation markersM de Eguileor
DBSF University of Insubria, Varese, Italy
Tissue Cell 32:437-45. 2000..CD61 and CD68 both recognised a band of about 110 kD; CD11b recognised a band of 200 kD, and CD11c, a band of 180 kD... - Detection and identification of antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testingI Peene
Department of Rheumatology, Ghent University Hospital, Belgium
Ann Rheum Dis 60:1131-6. 2001..CONCLUSIONS: The data from this analysis are useful in estimating the probabilities of detecting specific ANA. Line immunoassay was shown to be a sensitive test for the detection of anti-ENA antibodies... - CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytesSebastien Vankoningsloo
Laboratory of Biochemistry and Cellular Biology, University of Namur F U N D P, rue de Bruxelles, 61, 5000 Namur, Belgium
J Cell Sci 119:1266-82. 2006..These results highlight a new role for CREB in the control of triglyceride metabolism during the adaptative response of preadipocytes to mitochondrial dysfunction... - Characterization of barrier properties and inducible VEGF expression of several types of retinal pigment epithelium in medium-term culturePete Geisen
Department of Ophthalmology, University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599, USA
Curr Eye Res 31:739-48. 2006..For disease, use of RPE cells that do not require long-term culture are more efficient and may be more relevant to study certain pathologies... - Expression, subcellular localization, and regulation of sigma receptor in retinal muller cellsGuoliang Jiang
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912 2000, USA
Invest Ophthalmol Vis Sci 47:5576-82. 2006..This study examined the expression, subcellular localization, binding activity, and regulation of sigmaR1 in retinal Müller cells... - Heat shock induces preferential translation of ERGIC-53 and affects its recycling pathwayCarmen Spatuzza
Dipartimento di Biochimica e Biotecnologie Mediche, Universita degli Studi di Napoli Federico II, I 80131, Naples, Italy
J Biol Chem 279:42535-44. 2004..These results support the view that ERGIC-53 alone, or in association with MCFD2, plays important functions during cellular response to stress conditions... - Noncanonical cAMP pathway and p38 MAPK mediate beta2-adrenergic receptor-induced IL-6 production in neonatal mouse cardiac fibroblastsFeng Yin
Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100083, China
J Mol Cell Cardiol 40:384-93. 2006..The present results suggest that beta(2)-AR mediates IL-6 production through a noncanonical cAMP responsible pathway and p38 MAPK... - The complement system plays a critical role in the development of experimental autoimmune anterior uveitisPurushottam Jha
Department of Ophthalmology, Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
Invest Ophthalmol Vis Sci 47:1030-8. 2006..The role of complement in ocular autoimmunity was explored in a experimental autoimmune anterior uveitis (EAAU) animal model... - Impaired responses of leukemic dendritic cells derived from a human myeloid cell line to LPS stimulationKwang Dong Kim
Department of Biological Sciences, Sookmyung Women's University, Seoul 140-742, Korea
Exp Mol Med 38:72-84. 2006..Taken together, our findings suggest that, although MuDC express TLR4, unlike TNF-alpha and IL-1beta, LPS does not stimulate MuDC to acquire mature phenotypes, and they may have impaired activity to initiate innate immune response... - Involvement of SMAD4, but not of SMAD2, in transforming growth factor-beta1-induced trophoblast expression of matrix metalloproteinase-2Hai Yan Lin
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Front Biosci 11:637-46. 2006..However, retrovirus-mediated sense Smad2 gene transfer had no effect. These findings suggest that Smad4, but not Smad2, mediates TGF-beta1-induced MMP-2 expression in invasive extravillous trophoblasts... - A novel role for the adaptor molecule CD2-associated protein in transforming growth factor-beta-induced apoptosisMario Schiffer
Department of Medicine, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10461, USA
J Biol Chem 279:37004-12. 2004..Together, our in vitro and in vivo findings suggest that TGF-beta-induced podocyte apoptosis is an early pathomechanism in mice developing focal-segmental glomerulosclerosis associated with functional impairment of CD2AP... - Serodiagnosis of canine Babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant P50 expressed in Escherichia coliShinya Fukumoto
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
J Parasitol 90:387-91. 2004..These results indicate that the recombinant P50 expressed in E. coil is a useful diagnostic antigen for practical use in the diagnosis of B. gibsoni infection in dogs... - RNA interference targeting transforming growth factor-beta type II receptor suppresses ocular inflammation and fibrosisHiroshi Nakamura
Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, 60612, USA
Mol Vis 10:703-11. 2004..A direct application of siRNA into eyes to downregulate TbetaRII expression may provide a novel therapy for preventing ocular inflammation and scarring... - Predominance of Trypanosoma rangeli infection in children from a Chagas disease endemic area in the west-shore of the Panama canalAzael Saldaña
Gorgas Memorial Institute of Health Studies, PO Box 0816 02593, Panama 5, Republic of Panama
Mem Inst Oswaldo Cruz 100:729-31. 2005..7% for trypanosome infections, a seroprevalence of 2.9% against T. cruzi and a predominance of T. rangeli infection (6.8%). The immunological and clinical implications of these findings are discussed... - Novel splicing variant of mouse Orc1 is deficient in nuclear translocation and resistant for proteasome-mediated degradationYasuyuki Miyake
Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
J Biol Chem 280:12643-52. 2005.... - Icariin-mediated modulation of cell cycle and p53 during cardiomyocyte differentiation in embryonic stem cellsDanyan Zhu
Department of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China
Eur J Pharmacol 514:99-110. 2005..Results showed p53 to be an important regulator in the differentiation in embryonic stem cells treated with 10(-7) M icariin, controlling or adjusting the balance between differentiated cells and cells undergoing apoptosis... - Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitorsGabriela Kania
Institute of Plant Genetics and Crop Plant Research IPK, Corrensstr 3, D 06466 Gatersleben, Germany
Stem Cells 23:791-804. 2005..In conclusion, we present the somatic stem cell marker prominin-1 as a new parameter to define ES-derived committed and early progenitor cells... - Production and characterization of a monoclonal antibody specific for NS3 protease and the ATPase region of Dengue-2 virusJ Garcia-Cordero
Departamento de Biomedicina Molecular, CINVESTAV IPN, Mexico City, Mexico
Hybridoma (Larchmt) 24:160-4. 2005..We conclude that these antibodies may be a useful tool, not only to study the replicative process of Dengue virus, but also to generate specific diagnostic tools for Dengue infection... - Development and characterization of human constitutive proteasome and immunoproteasome subunit-specific monoclonal antibodiesN Bandoh
Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
Tissue Antigens 66:185-94. 2005..These results suggest that the constitutive proteasome and immunoproteasome subunit-specific mAbs we have developed are useful probes to characterize the expression of proteasome subunits in normal tissues and in pathological lesions... - Hypoxia induces the expression of membrane-type 1 matrix metalloproteinase in retinal glial cellsKousuke Noda
Department of Pathology, Keio University School of Medicine, Tokyo, Japan
Invest Ophthalmol Vis Sci 46:3817-24. 2005..CONCLUSIONS: Hypoxia can induce MT1-MMP expression in retinal glial cells, and the hypoxia-induced expression of MT1-MMP is mediated by VEGF in an autocrine fashion... - Rapid apoptosis induction by IGFBP-3 involves an insulin-like growth factor-independent nucleomitochondrial translocation of RXRalpha/Nur77Kuk Wha Lee
Division of Pediatric Endocrinology, Mattel Children s Hospital at UCLA, David Geffen School of Medicine, Los Angeles, California 90095, USA
J Biol Chem 280:16942-8. 2005..This unique nongenotropic nuclear pathway supports an emerging role for IGFBP-3 as a novel, multicompartmental signaling molecule involved in induction of apoptosis in malignant cells... - Entamoeba histolytica: identification of a distinct beta2 integrin-like molecule with a potential role in cellular adherenceDylan R Pillai
Division of Infectious Diseases, Department of Medicine, University of California, San Francisco 94143, USA
Exp Parasitol 109:135-42. 2005..The presence of integrin-dependent binding may allow trophozoites to opportunistically adhere to activated intestinal epithelium or vascular endothelium expressing ICAM-1 during amebic colitis or hepatic abscess... - Sequential molecular and cellular events during neoplastic progression: a mouse syngeneic ovarian cancer modelPaul C Roberts
Department of Immunology Microbiology, School of Medicine, Wayne State University, Detroit, MI 48201, USA
Neoplasia 7:944-56. 2005.... - PAF-induced furin and MT1-MMP expression is independent of MMP-2 activation in corneal myofibroblastsPaulo Ottino
Department of Ophthalmology and Neuroscience Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
Invest Ophthalmol Vis Sci 46:487-96. 2005..Thus, PAF-mediated changes in extracellular matrix composition surrounding the myofibroblasts could be important in regulating the corneal scarring process. Moreover, PAF antagonists could be useful in maintaining corneal transparency... - LBP and CD14 secreted in tears by the lacrimal glands modulate the LPS response of corneal epithelial cellsDavid R Blais
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ontario, Canada
Invest Ophthalmol Vis Sci 46:4235-44. 2005..The complementation of these tear and corneal immune proteins could play an important role in LPS recognition and signaling and, therefore, could modulate ocular innate immunity... - Plasmodium yoelii: axenic development of the parasite mosquito stagesJohanna M Porter-Kelley
Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
Exp Parasitol 112:99-108. 2006..Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations... - Elaboration of a crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-specific antibodies in goats from Sri LankaA Naguleswaran
Institute of Parasitology, University of Bern, Laenggass-Str. 122, CH-3001 Bern, Switzerland
Vet Parasitol 126:257-62. 2004..caninum antibodies. The optimal correlation between ELISA, IFAT, and Western blot confirms the suitability of the ELISA for large-scale seroepidemiologic studies, not only in cattle but also in goats... - Tissue-type plasminogen activator acts as a cytokine that triggers intracellular signal transduction and induces matrix metalloproteinase-9 gene expressionKebin Hu
Department of Pathology, University of Pittsburgh School of Medicine, S 405 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15261, USA
J Biol Chem 281:2120-7. 2006.... - TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expressionSusan G Elner
Department of Ophthalmology, University of Michigan, W. K. Kellogg Eye Center, Ann Arbor 48105, USA
Invest Ophthalmol Vis Sci 46:4627-33. 2005..These roles indicate potential involvement in retinal degenerative and inflammatory processes... - Autoimmune subepidermal blistering diseases in Uganda: correlation of autoantibody class with age of patientsGrace K Mulyowa
Department of Dermatology, , , Germany
Int J Dermatol 45:1047-52. 2006..The high frequency of IgA autoantibodies in Ugandan patients may be explained by the age distribution of the Ugandan population... - Hypoxic damage to the periventricular white matter in neonatal brain: role of vascular endothelial growth factor, nitric oxide and excitotoxicityCharanjit Kaur
Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
J Neurochem 98:1200-16. 2006..Our findings point towards the involvement of excitotoxicity, VEGF and NO in periventricular white matter damage in response to hypoxia... - Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extractHua He
TissueTech, Inc and the Ocular Surface Center, Miami, Florida 33173, USA
Invest Ophthalmol Vis Sci 49:4468-75. 2008..Experimentally, the authors and others have reported that AM facilitates macrophage apoptosis. However, it remains unclear whether such anti-inflammatory activity is retained in AM extract (AME)... - p53 expression in the normal murine eyeRussell Pokroy
Department of Ophthalmology, Kaplan Medical Center, Rehovot, Israel
Invest Ophthalmol Vis Sci 43:1736-41. 2002..CONCLUSIONS: High levels of p53 protein are found in various normal murine ocular tissues, especially the corneal and conjunctival structures and the lens epithelium. Each of these tissues demonstrate unique patterns of staining... - Tumor necrosis factor-alpha-induced apoptosis in murine cytomegalovirus retinitisJun Zhou
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA
Invest Ophthalmol Vis Sci 48:1691-700. 2007..To explore the mechanism underlying retinal apoptosis in MCMV retinitis, this study was initiated to determine whether the tumor necrosis factor receptor (TNFR)1-TNF pathway is involved in apoptosis during MCMV retinitis... - Exogenous expression of E-cadherin in gallbladder carcinoma cell line G-415 restores its cellular polarity and differentiationW Jiao
Department of Surgery, Saga Medical School, Saga 849-8501, Japan
Int J Oncol 19:1099-107. 2001..Simultaneous translocation of alpha-, beta-catenin to membrane following functional E-cad expression may be a critical event for this process... - The angiogenic peptide vascular endothelial growth factor is expressed in foetal and ruptured tendonsT Pufe
University of Kiel, Department of Anatomy, Germany
Virchows Arch 439:579-85. 2001..These results prove the presence of an angiogenic peptide and vascularisation in ruptured and foetal tendons and support the view that microtrauma or degeneration in the Achilles tendon precedes its rupture... - Expression of cyclooxygenase-1 and -2 in normal and glaucomatous human eyesC Maihofner
Department of Experimental and Clinical Pharmacology and Toxicology, University of Erlangen Nurnberg, Germany
Invest Ophthalmol Vis Sci 42:2616-24. 2001.... - Attenuated glial reactions and photoreceptor degeneration after retinal detachment in mice deficient in glial fibrillary acidic protein and vimentinToru Nakazawa
Angiogenesis Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA
Invest Ophthalmol Vis Sci 48:2760-8. 2007.... - Role of NF-kappaB and MAPKs in light-induced photoreceptor apoptosisLi Ping Yang
Peking University Eye Center, Peking University Third Hospital, Peking University, Beijing, China
Invest Ophthalmol Vis Sci 48:4766-76. 2007.... - Expression of prolactin gene and secretion of prolactin by rat retinal capillary endothelial cellsA Ochoa
Neurobiology Center, National Autonomous University of Mexico, , Mexico
Invest Ophthalmol Vis Sci 42:1639-45. 2001..That the cloned prolactin receptor was not expressed in these cells argues against direct autocrine effects of prolactin. Possible paracrine effects are suggested by the expression of the prolactin receptor in retinal tissue... - Impaired retinal iron homeostasis associated with defective phagocytosis in Royal College of Surgeons ratsMarina G Yefimova
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia
Invest Ophthalmol Vis Sci 43:537-45. 2002..The increase of iron in the photoreceptor area may enhance the vulnerability of cells to oxidative stress...
Research Grants
- LYME DISEASE IN WESTERN USA--ECOLOGY AND EPIDEMIOLOGYRobert Lane; Fiscal Year: 1992..The effects of exposure to direct sunlight or of spirochetal infection on questing by immature ticks also will be determined...
- HSV-1 PROCESSING AND PACKAGING GENESSandra Weller; Fiscal Year: 1999..It is anticipated that an analysis of the processing/packaging proteins will not only provide an understanding of the mechanisms of their action but may also lead to the development of novel strategies for antiviral therapy. ..
- COPPER HOMEOSTASIS IN YEASTDennis Thiele; Fiscal Year: 1999..The experiments proposed in this application are aimed at elucidating the fundamental processes by which yeast, and all organisms, establish and maintain normal Cu homeostasis. ..
- Regulation of GABAA Receptor Cell Surface ExpressionNANCY LEIDENHEIMER; Fiscal Year: 2006..The involvement of GABAA receptors in anxiety, sleep and seizure disorders underscores the importance of understanding the cellular and molecular mechanisms of this receptor. ..
- A GENE FAMILY EXPRESSED IN SEA URCHIN EMBRYONIC ECTODERMWILLIAM KLEIN; Fiscal Year: 1992..Antibodies against the Spec3 protein will be generated from the Spec3 ORF and the location of the protein in urchin embryos will be determined by immunological detection...
- Immunofluorescence Standards for Flow CytometryDouglas Redelman; Fiscal Year: 2003..abstract_text> ..
- INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTIONDonald Defranco; Fiscal Year: 1993..Through these studies we hope to gain a better understanding of how regulated modification of a signal transducing protein influences its subcellular compartmentalization and association with ancillary factors that modify its function...
- MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEASTMichael Snyder; Fiscal Year: 1993..and role of these proteins in nucleating microtubule assembly will be examined.' The long term goal of these studies is to understand at a molecular level the components and events necessary for successful mitosis and cell growth...