tetracycline

Summary

Summary: A naphthacene antibiotic that inhibits AMINO ACYL TRNA binding during protein synthesis.

Top Publications

  1. ncbi Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution
    M C Roberts
    Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle 98195 7238, USA
    FEMS Microbiol Rev 19:1-24. 1996
  2. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
  3. ncbi Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells
    Caroline Beard
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genesis 44:23-8. 2006
  4. ncbi Update on acquired tetracycline resistance genes
    Marilyn C Roberts
    Department of Pathobiology, Box 357238, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
    FEMS Microbiol Lett 245:195-203. 2005
  5. ncbi Generation of cell lines with tetracycline-regulated autophagy and a role for autophagy in controlling cell size
    Nao Hosokawa
    Department of Bioregulation and Metabolism, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Tokyo 113 8613, Japan
    FEBS Lett 580:2623-9. 2006
  6. ncbi Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery
    Terry Roemer
    Elitra Canada, Montreal, Quebec, Canada, H2X 3Y8
    Mol Microbiol 50:167-81. 2003
  7. pmc A tetracycline-repressible transactivator system to study essential genes in malaria parasites
    Paco Pino
    Department of Microbiology and Molecular Medicine, CMU, University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    Cell Host Microbe 12:824-34. 2012
  8. ncbi Conditional control of gene expression in the mouse
    M Lewandoski
    Section of Genetics of Vertebrate Development, Laboratory of Cancer and Developmental Biology, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
    Nat Rev Genet 2:743-55. 2001
  9. pmc Tetracycline-inducible gene expression and gene deletion in Candida albicans
    Yang Nim Park
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Eukaryot Cell 4:1328-42. 2005
  10. pmc Tetracycline treatment retards the onset and slows the progression of diabetes in human amylin/islet amyloid polypeptide transgenic mice
    Jacqueline F Aitken
    School of Biological Sciences, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
    Diabetes 59:161-71. 2010

Detail Information

Publications333 found, 100 shown here

  1. ncbi Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution
    M C Roberts
    Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle 98195 7238, USA
    FEMS Microbiol Rev 19:1-24. 1996
    b>Tetracycline-resistant bacteria were first isolated in 1953 from Shigella dysenteriae, a bacterium which causes bacterial dysentery. Since then tetracycline-resistant bacterial have been found in increasing numbers of species and genera...
  2. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
    ..The tetracycline-inducible opsin expression system was assembled into this GnTI(-) HEK293S cell line...
  3. ncbi Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells
    Caroline Beard
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genesis 44:23-8. 2006
    ..by site-specific recombination that allows establishment of multiple embryonic stem (ES) cell lines carrying tetracycline-inducible genes targeted to a specific locus to assure predictable temporal and spatial expression in ES cells ..
  4. ncbi Update on acquired tetracycline resistance genes
    Marilyn C Roberts
    Department of Pathobiology, Box 357238, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
    FEMS Microbiol Lett 245:195-203. 2005
    This mini-review summarizes the changes in the field of bacterial acquired tetracycline resistance (tet) and oxytetracycline (otr) genes identified since the last major review in 2001...
  5. ncbi Generation of cell lines with tetracycline-regulated autophagy and a role for autophagy in controlling cell size
    Nao Hosokawa
    Department of Bioregulation and Metabolism, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Tokyo 113 8613, Japan
    FEBS Lett 580:2623-9. 2006
    ..The generated cell lines will be useful reagents for future mechanistic studies into the regulation and physiologic significance of autophagy...
  6. ncbi Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery
    Terry Roemer
    Elitra Canada, Montreal, Quebec, Canada, H2X 3Y8
    Mol Microbiol 50:167-81. 2003
    ..albicans essential gene set, and their respective conditional mutant strains may be directly used as sensitive whole-cell assays for drug screening...
  7. pmc A tetracycline-repressible transactivator system to study essential genes in malaria parasites
    Paco Pino
    Department of Microbiology and Molecular Medicine, CMU, University of Geneva, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    Cell Host Microbe 12:824-34. 2012
    ..and mosquito-stage parasites and used to create a robust conditional expression system for stage-specific, tetracycline-dependent gene regulation in Toxoplasma gondii, Plasmodium berghei, and Plasmodium falciparum...
  8. ncbi Conditional control of gene expression in the mouse
    M Lewandoski
    Section of Genetics of Vertebrate Development, Laboratory of Cancer and Developmental Biology, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
    Nat Rev Genet 2:743-55. 2001
    ..Here, I review these systems and discuss certain studies that exemplify the advantages and limitations of each system...
  9. pmc Tetracycline-inducible gene expression and gene deletion in Candida albicans
    Yang Nim Park
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, Rontgenring 11, D 97070 Wurzburg, Germany
    Eukaryot Cell 4:1328-42. 2005
    ..We have established a system that allows induction of gene expression in C. albicans by the addition of tetracycline (Tet)...
  10. pmc Tetracycline treatment retards the onset and slows the progression of diabetes in human amylin/islet amyloid polypeptide transgenic mice
    Jacqueline F Aitken
    School of Biological Sciences, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
    Diabetes 59:161-71. 2010
    ..Here, we used tetracycline, which modifies hA/hIAPP oligomerization, to probe mechanisms whereby hA/hIAPP causes diabetes in hemizygous hA/..
  11. ncbi Bismuth-based quadruple therapy using a single capsule of bismuth biskalcitrate, metronidazole, and tetracycline given with omeprazole versus omeprazole, amoxicillin, and clarithromycin for eradication of Helicobacter pylori in duodenal ulcer patients: a
    Loren Laine
    University of Southern California School of Medicine, Los Angeles, CA 90033, USA
    Am J Gastroenterol 98:562-7. 2003
    ..assessed efficacy of bismuth-based quadruple therapy with omeprazole, bismuth biskalcitrate, metronidazole, and tetracycline (OBMT) using a single-triple capsule of BMT compared with triple therapy with omeprazole, amoxicillin, and ..
  12. pmc An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast
    G Belli
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198 Lleida, Spain
    Nucleic Acids Res 26:942-7. 1998
    ..This combination allows tight expression of tetO- driven genes, both in a direct (tetracycline-repressible) and reverse (tetracycline-inducible) dual system...
  13. ncbi Tissue-specific and reversible RNA interference in transgenic mice
    Ross A Dickins
    Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Nat Genet 39:914-21. 2007
    ..By adapting the tetracycline (tet)-responsive system previously used for gene overexpression, we have developed a simple transgenic system ..
  14. ncbi The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit
    D E Brodersen
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 103:1143-54. 2000
    ..atomic structure of the 30S ribosomal subunit to determine the structures of its complexes with the antibiotics tetracycline, pactamycin, and hygromycin B...
  15. ncbi Conditional tetracycline-regulated expression of TGF-beta1 in liver of transgenic mice leads to reversible intermediary fibrosis
    Elke Ueberham
    University of Leipzig, Institute for Biochemistry, Germany
    Hepatology 37:1067-78. 2003
    Based on the tetracycline-regulated gene expression system, a double-transgenic mouse model for liver fibrosis was established in which the expression of transforming growth factor beta1 (TGF-beta1) can be regulated deliberately by ..
  16. ncbi Occurrence and transport of tetracycline, sulfonamide, quinolone, and macrolide antibiotics in the Haihe River Basin, China
    Yi Luo
    Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Sciences and Engineering, Nankai University, Wei Jin Road 94, Tianjin 300071, China
    Environ Sci Technol 45:1827-33. 2011
    The occurrence and transport of 12 antibiotics (from the tetracycline, sulfonamide, quinolone, and macrolide families) was studied in a 72-km stretch of the Haihe River, China, and in six of its tributaries...
  17. pmc Global changes in Kaposi's sarcoma-associated virus gene expression patterns following expression of a tetracycline-inducible Rta transactivator
    Hiroyuki Nakamura
    Department of Microbiology and Molecular Genetics, Division of Tumor Virology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA
    J Virol 77:4205-20. 2003
    ..This system uses Flp-mediated efficient recombination and tetracycline-inducible expression...
  18. pmc Strain background influences neurotoxicity and behavioral abnormalities in mice expressing the tetracycline transactivator
    Harry J Han
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    J Neurosci 32:10574-86. 2012
    ..The utility of this system lies in the assumption that the tetracycline transactivator (TTA) acts as an inert control element and does not contribute to phenotypes under study...
  19. pmc Chicken faecal microbiota and disturbances induced by single or repeated therapy with tetracycline and streptomycin
    Petra Videnska
    Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic
    BMC Vet Res 9:30. 2013
    ..we characterised the microbiota present in the faeces of 15- and 46-week-old egg laying hens before and after tetracycline or streptomycin therapy. In the first experiment, the layers were subjected to 7 days of therapy...
  20. pmc Influence of soil use on prevalence of tetracycline, streptomycin, and erythromycin resistance and associated resistance genes
    Magdalena Popowska
    Department of Applied Microbiology, Institute of Microbiology, University of Warsaw, Warsaw, Poland
    Antimicrob Agents Chemother 56:1434-43. 2012
    ..The most frequently detected resistance genes were tet(B), tet(D), tet(O), tet(T), and tet(W) for tetracycline resistance, str(A), str(B), and aac for streptomycin resistance, and erm(C), erm(V), erm(X), msr(A), ole(B), ..
  21. ncbi Multiplex PCR for the detection of tetracycline resistant genes
    L K Ng
    National Microbiology Laboratory, Population and Public Health Branch, Winnipeg, MB, R3E 3R2, Canada
    Mol Cell Probes 15:209-15. 2001
    Specific primer pairs were selected for the PCR amplification of 14 tetracycline resistant genes commonly found in Gram positive and Gram negative organisms...
  22. pmc A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor
    S K Poon
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Open Biol 2:110037. 2012
    Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into Trypanosoma brucei...
  23. ncbi Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance
    Xuanxian Peng
    Center for Proteomics, Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, People s Republic of China
    J Proteome Res 4:2257-65. 2005
    ..aeruginosa responding to ampicilin, kanamycin and tetracycline resistances...
  24. ncbi Transgenic calmodulin-dependent protein kinase II activation: dose-dependent effects on synaptic plasticity, learning, and memory
    Rafael Bejar
    Neurosciences Graduate Program, University of California at San Diego, La Jolla, California 92093, USA
    J Neurosci 22:5719-26. 2002
    ..We used transgenic mice carrying a tetracycline-regulated, calcium-independent form of CaMKII (CaMKII-Asp286) to investigate the role of CaMKII activation on ..
  25. pmc erm(B)-carrying elements in tetracycline-resistant pneumococci and correspondence between Tn1545 and Tn6003
    Ileana Cochetti
    Institute of Microbiology and Biomedical Sciences, Polytechnic University of Marche Medical School, Via Tronto 10 A, 60020 Ancona, Italy
    Antimicrob Agents Chemother 52:1285-90. 2008
    ..the genetic organization of erm(B)-carrying transposons of Streptococcus pneumoniae and their distribution in tetracycline-resistant clinical isolates...
  26. ncbi Hepatic differentiation of human bone marrow-derived mesenchymal stem cells by tetracycline-regulated hepatocyte nuclear factor 3beta
    Kyoko Ishii
    Department of Genetic Medicine and Regenerative Therapeutics, Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University, Yonago, Japan
    Hepatology 48:597-606. 2008
    ..In the present study, we established a tetracycline (Tet)-regulated expression system for HNF3beta in UE7T-13 BM-MSCs...
  27. ncbi Abundance of six tetracycline resistance genes in wastewater lagoons at cattle feedlots with different antibiotic use strategies
    Nicholas Peak
    Department of Civil, Environmental, and Architectural Engineering, University of Kansas, Lawrence, KS 66045, USA
    Environ Microbiol 9:143-51. 2007
    The abundance of six tetracycline resistance genes tet(O), tet(Q), tet(W), tet(M), tet(B) and tet(L), were quantified over time in wastewater lagoons at concentrated animal feeding operations (CAFO) to assess how feedlot operation affects ..
  28. pmc Silencing and un-silencing of tetracycline-controlled genes in neurons
    Peixin Zhu
    Max Planck Institute for Medical Research, Heidelberg, Germany
    PLoS ONE 2:e533. 2007
    To identify the underlying reason for the controversial performance of tetracycline (Tet)-controlled regulated gene expression in mammalian neurons, we investigated each of the three components that comprise the Tet inducible systems, ..
  29. ncbi A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells
    S Freundlieb
    Zentrum für Molekulare Biologie ZMBH, Universitat Heidelberg, Germany
    J Gene Med 1:4-12. 1999
    ..transcription can, however, be observed whenever integration of target genes driven by promoters responsive to tetracycline controlled transcriptional activators (tTA, rtTA) does not occur at suitable chromosomal sites...
  30. ncbi Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system
    G Belli
    Departament de Ciencies Mediques Basiques, Facultat de Medicina, Universitat de Lleida, Spain
    Yeast 14:1127-38. 1998
    ..cassette has been constructed that allows one-step substitution of chromosomal gene promoters for the tetracycline-regulatable tetO promoter in yeast cells, which uses kanMX4 as selective marker for geneticin resistance...
  31. pmc Silencing Mycobacterium smegmatis by using tetracycline repressors
    Xinzheng V Guo
    Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10021, USA
    J Bacteriol 189:4614-23. 2007
    ..processes in mycobacteria, we and others have developed regulated expression systems that are repressed by a tetracycline repressor (TetR) and induced with tetracyclines, permitting the construction of conditional mutants of ..
  32. ncbi Adsorption of tetracycline on single-walled and multi-walled carbon nanotubes as affected by aqueous solution chemistry
    Liangliang Ji
    State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Jiangsu 210093, China
    Environ Toxicol Chem 29:2713-9. 2010
    ..The present study investigated the influence of aqueous solution chemistry on the adsorption of tetracycline to carbon nanotubes...
  33. ncbi Pooled analysis on the efficacy of the second-line treatment regimens for Helicobacter pylori infection
    M Hojo
    Dept. of Gastroenterology, Juntendo University, School of Medicine, Tokyo, Japan
    Scand J Gastroenterol 36:690-700. 2001
    ..The strategy of adding two new antimicrobials to previous regimens was also effective in re-eradication therapy...
  34. ncbi A retroviral expression system based on tetracycline-regulated tricistronic transactivator/repressor vectors for functional analyses of antiproliferative and toxic genes
    Michael J Ausserlechner
    Molecular Biology Research Laboratory, Pediatric Department, Medical University Innsbruck, Innrain 66, A 6020 Innsbruck, Austria
    Mol Cancer Ther 5:1927-34. 2006
    ..To overcome such limitations, we designed a gene expression system that transcribes the tetracycline-dependent rtTA2-M2-activator, TRSID-silencer, and selection marker as a tricistronic mRNA from a single ..
  35. pmc An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit
    Shinji Masui
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology Minatojima minamimachi 2 2 3, Kobe, Japan
    Nucleic Acids Res 33:e43. 2005
    ..of the ROSA-TET system, an effective system for the establishment of multiple ES cell lines carrying a tetracycline (Tc)-regulatable transgene at the Gt (ROSA)26asSor (ROSA26) locus...
  36. ncbi Evidence for the predominance of a single tet(M) gene sequence type in tetracycline-resistant Ureaplasma parvum and Mycoplasma hominis isolates from Tunisian patients
    Boutheina Ben Abdelmoumen Mardassi
    Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, 13 Place Pasteur, B P 74, 1002 Tunis Belvedere, Tunis, Tunisia
    J Med Microbiol 61:1254-61. 2012
    ..Nucleotide sequence analysis of the tet(M) amplicon revealed a unique sequence shared by all tetracycline-resistant clinical isolates of both species. Molecular typing indicated that the tetracycline-resistant U...
  37. pmc Molecular characterization of a novel mosaic tet(S/M) gene encoding tetracycline resistance in foodborne strains of Streptococcus bovis
    Simona Barile
    INRAN National Research Institute on Food and Nutrition, Via Ardeatina 546, 00178 Roma, Italy
    Microbiology 158:2353-62. 2012
    ..We have focused on the molecular characterization of a tetracycline (Tet)-resistance gene present in 39 foodborne isolates of S. bovis phenotypically resistant to this drug...
  38. ncbi Tetracycline-resistant Chlamydia suis in cases of reproductive failure on Belgian, Cypriote and Israeli pig production farms
    Katelijn Schautteet
    Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    J Med Microbiol 62:331-4. 2013
    ..Nineteen of 23 (83 %) C. suis-positive sows were infected with tetracycline-resistant C. suis strains, as determined by MIC tests...
  39. ncbi The trends of one-week first-line and second-line eradication therapy for Helicobacter pylori infection in Korea
    Jun Won Chung
    Department of Internal Medicine, Gachon University, Gil Medical Center, Incheon
    Hepatogastroenterology 58:246-50. 2011
    ..The aim of this study was to evaluate 7-year changes in efficacy of one-week first line and second line therapies in Helicobacter pylori eradication rates in Korea...
  40. ncbi Correlation of tetracycline and sulfonamide antibiotics with corresponding resistance genes and resistant bacteria in a conventional municipal wastewater treatment plant
    Pin Gao
    College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China
    Sci Total Environ 421:173-83. 2012
    ..In this study, total concentrations of tetracycline and sulfonamide antibiotics in final effluent were detected at 652.6 and 261...
  41. ncbi Resistance of helicobacter pylori to metronidazole, tetracycline and amoxycillin
    H Wu
    Department of Microbiology, Shanghai Second Medical University, Chongqing South Road 280, China 200025
    J Antimicrob Chemother 46:121-3. 2000
    Resistance to metronidazole, tetracycline and amoxycillin, and beta-lactamase production were determined for 153 clinical isolates of Helicobacter pylori. Of these isolates, 77.8% were resistant to metronidazole (MIC > 8 mg/L), 58...
  42. ncbi Efficacy and safety of single-triple capsules of bismuth biskalcitrate, metronidazole and tetracycline, given with omeprazole, for the eradication of Helicobacter pylori: an international multicentre study
    C O'Morain
    Department of Gastroenterology, Adelaide and Health Hospital, Tallaght, Dublin, Ireland
    Aliment Pharmacol Ther 17:415-20. 2003
    ..The high prevalence of Helicobacter pylori resistance to metronidazole demands treatments more effective than standard bismuth-based triple therapy against these strains...
  43. ncbi Occurrence of two genotypes of tetracycline (TC) resistance gene tet(M) in the TC-resistant bacteria in marine sediments of Japan
    M Habibur Rahman
    Center for Marine Environmental Studies, Ehime University, Matsuyama 790 8577, Japan
    Environ Sci Technol 42:5055-61. 2008
    The tetracycline (TC) resistance gene tet(M) was monitored in bacteria isolated from Japanese coastal and off-shore marine sediments...
  44. ncbi Inhibition of proteolytic, serpinolytic, and progelatinase-b activation activities of periodontopathogens by doxycycline and the non-antimicrobial chemically modified tetracycline derivatives
    D Grenier
    Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada
    J Periodontol 73:79-85. 2002
    ..In this study, the effects of Doxy and CMT-1, -3, and -5 on proteolytic, serpinolytic, and progelatinase-B activation activities of potent periodontopathogens were studied...
  45. ncbi Propensity of activated sludge to amplify or attenuate tetracycline resistance genes and tetracycline resistant bacteria: a mathematical modeling approach
    Sungpyo Kim
    Department of Earth and Environmental Engineering, Columbia University, 500 West 120th Street, New York, NY 10027, USA
    Chemosphere 78:1071-7. 2010
    ..of the activated sludge (AS) process at three wastewater treatment plants (WWTP) to amplify or attenuate tetracycline resistant bacteria (TRB) and tetracycline resistance genes (TRG)...
  46. pmc Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging
    Bradley A Creamer
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
    Genesis 47:234-45. 2009
    We generated a novel mouse model, which expresses the tetracycline-inducible transactivator under the regulation of the endogenous whey acidic protein gene...
  47. ncbi Filarial susceptibility and effects of Wolbachia in Aedes pseudoscutellaris mosquitoes
    T J Dutton
    Vector Research Group, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
    Med Vet Entomol 19:60-5. 2005
    ..Maternal inheritance of filarial susceptibility in the complex has previously been asserted, and larval tetracycline treatment reduced susceptibility; the maternally inherited Wolbachia in these mosquitoes were suggested to be ..
  48. ncbi Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor
    Felix P Eckenstein
    Department of Neurology and Department of Anatomy and Neurobiology, College of Medicine, University of Vermont, HSRF 408, VT 05405, USA
    Exp Neurol 198:338-49. 2006
    ..EMBO J 9 833-840] can be used to drive widespread neuronal expression of the reverse tetracycline transactivator M2 (rtTA-M2 [Urlinger, S., Baron, U., Thellmann, M., Hasan, M.T., Bujard, H., and Hillen, W...
  49. ncbi The power of reversibility regulating gene activities via tetracycline-controlled transcription
    Kai Schonig
    Zentralinstitut fur Seelische Gesundheit, Mannheim, Germany
    Methods Enzymol 477:429-53. 2010
    b>Tetracycline-controlled transcriptional activation systems are widely used to control gene expression in transgenic animals in a truly conditional manner...
  50. ncbi Effects of tetracycline and zinc on selection of methicillin-resistant Staphylococcus aureus (MRSA) sequence type 398 in pigs
    Arshnee Moodley
    Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, 1870, Denmark
    Vet Microbiol 152:420-3. 2011
    An in vivo experiment was conducted to evaluate the effects of tetracycline and zinc on pig colonization and transmission of methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 398...
  51. pmc Tetracycline and streptomycin resistance genes, transposons, and plasmids in Salmonella enterica isolates from animals in Italy
    Cristina Pezzella
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    Antimicrob Agents Chemother 48:903-8. 2004
    ..of 20 serotypes, isolated from animal sources in Italy, were analyzed for tet(A) and strA-strB, conferring tetracycline and streptomycin resistance, respectively...
  52. ncbi Tetracyclines and chemically modified tetracycline-3 (CMT-3) modulate cytokine secretion by lipopolysaccharide-stimulated whole blood
    Julia Cazalis
    Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec City, Quebec, G1K 7P4, Canada
    Inflammation 32:130-7. 2009
    ..In the present study, we investigated the effects of tetracycline (TC), doxycycline (doxy), and chemically modified tetracycline-3 (CMT-3) on the production of pro-inflammatory ..
  53. pmc Tet(M)-promoted release of tetracycline from ribosomes is GTP dependent
    V Burdett
    Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 178:3246-51. 1996
    ..factor G (EF-G), interacts with the protein biosynthetic machinery to render this process resistant to tetracycline in vivo and in vitro...
  54. pmc The TetR family of transcriptional repressors
    Juan L Ramos
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Microbiol Mol Biol Rev 69:326-56. 2005
    ..e., TetR bound to the target operator represses tetA transcription and is released in the presence of tetracycline), or more complex, involving a series of regulatory cascades in which either the expression of the TetR family ..
  55. ncbi Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma brucei
    Bill Wickstead
    School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    Mol Biochem Parasitol 125:211-6. 2002
  56. ncbi Effects of mouse strain, position of integration and tetracycline analogue on the tetracycline conditional system in transgenic mice
    Andrea Robertson
    Imperial College School of Science, Technology and Medicine, Division of Biomedical Sciences and Clinical Sciences Centre, London, UK
    Gene 282:65-74. 2002
    The tetracycline conditional system is a very powerful method for achieving control of gene expression in transgenic mice, allowing one to turn expression both off and on in the same animal...
  57. ncbi Tetracycline treatment and sex-ratio distortion: a role for Wolbachia in the moulting of filarial nematodes?
    M Casiraghi
    Dipartimento di Patologia Animale, Universita di Milano, Milan, Italy
    Int J Parasitol 32:1457-68. 2002
    ..These bacteria are thought to be beneficial to the host nematode. Indeed, tetracycline treatments reduce the population of Wolbachia in filarial worms and have detrimental effects on the nematode...
  58. ncbi Identification of tet(M) in two Lactococcus lactis strains isolated from a Spanish traditional starter-free cheese made of raw milk and conjugative transfer of tetracycline resistance to lactococci and enterococci
    Ana Belén Flórez
    Instituto de Productos Lácteos de Asturias CSIC, Carretera de Infiesto s n, 33300 Villaviciosa, Asturias, Spain
    Int J Food Microbiol 121:189-94. 2008
    Specific PCR and sequencing showed that a tet(M) gene was present in two tetracycline-resistant Lactococcus lactis strains isolated from a raw milk, starter-free cheese...
  59. ncbi Tetracycline resistance genes persist at aquaculture farms in the absence of selection pressure
    Manu Tamminen
    Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland
    Environ Sci Technol 45:386-91. 2011
    ..prophylactic and therapeutic use of tetracyclines in aquaculture has been shown to contribute to the spread of tetracycline resistance in the environment...
  60. ncbi An improved tetracycline-inducible expression vector for Staphylococcus aureus
    Rebecca M Corrigan
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Plasmid 61:126-9. 2009
    The tetracycline-inducible expression vector pALC2073 allowed high level expression of the cloned sasG gene but repression by uninduced cells was leaky...
  61. pdf The application of Tet repressor in prokaryotic gene regulation and expression
    Ralph Bertram
    Lehrbereich Mikrobielle Genetik, Eberhard Karls Universitat Tubingen, Waldhäuserstr 70 8, 72076 Tubingen, Germany
    Microb Biotechnol 1:2-16. 2008
    ..In its original environment, Tet repressor (TetR) negatively controls tetracycline (tc) resistance in bacteria...
  62. ncbi Resistance among Streptococcus pneumoniae: Implications for drug selection
    Peter C Appelbaum
    Departments of Pathology and Clinical Microbiology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA
    Clin Infect Dis 34:1613-20. 2002
    ..Efforts should be made to prevent pneumococcal infections in high-risk patients through vaccination...
  63. ncbi Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1
    S A Lacks
    J Mol Biol 192:753-65. 1986
    The streptococcal plasmid pMV158 and its derivative pLS1 are able to replicate and confer tetracycline resistance in both Gram-positive and Gram-negative bacteria...
  64. pmc Epigallocatechin-gallate enhances the activity of tetracycline in staphylococci by inhibiting its efflux from bacterial cells
    Andrea Sudano Roccaro
    SIFI SpA, Research and Development Department, Via E Patti 36, 95020 Lavinaio, Catania, Italy
    Antimicrob Agents Chemother 48:1968-73. 2004
    ..article, we report that EGCg at doses half and below its calculated MIC of 100 microg/ml, is able to reverse tetracycline resistance in staphylococcal isolates expressing the specific efflux pump Tet(K) and appears to improve the ..
  65. ncbi Levofloxacin-based triple therapy versus bismuth-based quadruple therapy for persistent Helicobacter pylori infection: a meta-analysis
    Richard J Saad
    Department of Medicine, Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Ann Arbor, MI 48109, USA
    Am J Gastroenterol 101:488-96. 2006
    ..Levofloxacin-based triple therapy has been suggested as an alternative salvage therapy to bismuth-based quadruple therapy for persistent Helicobacter pylori (H. pylori) infection...
  66. ncbi In vivo gene regulation using tetracycline-regulatable systems
    Knut Stieger
    INSERM UMR U649, CHU Hotel Dieu, Nantes, France
    Adv Drug Deliv Rev 61:527-41. 2009
    ..Among these, the tetracycline-regulatable system is by far the most frequently used and the most advanced towards gene therapy trials...
  67. pmc Molecular analysis of a synthetic tetracycline-binding riboswitch
    Shane Hanson
    Lehrstuhl fur Mikrobiologie, Fried rich Alexander Universität Erlangen Nürnberg, Staudtstrasse 5, 91058 Erlangen, Germany
    RNA 11:503-11. 2005
    ..Regulation by a tetracycline (tc)-binding aptamer when inserted into the 5' untranslated region (UTR) of a reporter gene exhibits all ..
  68. ncbi Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi
    Wanderson D DaRocha
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Caixa Postal 486, Belo Horizonte, Brazil
    Mol Biochem Parasitol 133:175-86. 2004
    ..We generated a strain of T. cruzi CL-Brener in which the T7 promoter and tetracycline operator could be used to maximize tetracycline-regulated dsRNA synthesis and constructed plasmids that direct ..
  69. pmc Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent
    Douglas Strathdee
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom Centre for Neuroscience Research, University of Edinburgh, Edinburgh, Scotland
    PLoS ONE 1:e4. 2006
    ..A single copy of the DNA construct can be inserted by targeting into regions of chromatin that allow the desired developmental and tissue-specific expression of the transgene...
  70. pmc Effect of tetracycline on transfer and establishment of the tetracycline-inducible conjugative transposon Tn916 in the guts of gnotobiotic rats
    Martin Iain Bahl
    Department of General Microbiology, University of Copenhagen, 1307 Copenhagen K, Denmark
    Appl Environ Microbiol 70:758-64. 2004
    ..The animals continuously received tetracycline in drinking water...
  71. ncbi A tetracycline-binding RNA aptamer
    C Berens
    Institute of Microbiology and Genetics, Vienna Biocenter Dr. Bohrgasse 9/4, A-1030, Vienna, Austria
    Bioorg Med Chem 9:2549-56. 2001
    ..To study the mode of interaction of tetracycline with RNA, we isolated aptamers with high affinity to this antibiotic via in vitro selection...
  72. ncbi Viral evolution as a tool to improve the tetracycline-regulated gene expression system
    Atze T Das
    Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Biol Chem 279:18776-82. 2004
    ..We used this approach to optimize the tetracycline (Tc)-regulated gene expression system (Tet system) for its function in mammalian cells...
  73. ncbi The tetracycline resistome
    Maulik Thaker
    Department of Biochemistry and Biomedical Sciences, Michael G DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Canada
    Cell Mol Life Sci 67:419-31. 2010
    Resistance to tetracycline emerged soon after its discovery six decades ago...
  74. ncbi Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background expression and improved dynamic range
    Niels Heinz
    Experimental Hematology, Hannover Medical School, Carl Neuberg Strasse 1, Hannover, Germany
    Hum Gene Ther 22:166-76. 2011
    ..efficient regulation of transgene expression requires tightly controlled inducible promoters, as shown for the tetracycline regulatory system (tet-system)...
  75. ncbi Distribution of tetracycline and streptomycin resistance genes and class 1 integrons in Enterobacteriaceae isolated from dairy and nondairy farm soils
    Velusamy Srinivasan
    Department of Animal Science and the Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN 37996, USA
    Microb Ecol 55:184-93. 2008
    The prevalence of selected tetracycline and streptomycin resistance genes and class 1 integrons in Enterobacteriaceae (n = 80) isolated from dairy farm soil and nondairy soils was evaluated...
  76. ncbi Initiation of actinorhodin export in Streptomyces coelicolor
    Kapil Tahlan
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada
    Mol Microbiol 63:951-61. 2007
    ..and actA resemble the classic tetR and tetA repressor/efflux pump-encoding gene pairs that confer resistance to tetracycline. Like TetR, which represses tetA, ActR is a repressor of actA...
  77. pmc Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast
    Julia E Weigand
    Lehrstuhl fur Mikrobiologie, Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, 91058 Erlangen, Germany
    Nucleic Acids Res 35:4179-85. 2007
    ..We apply the synthetic tetracycline (tc)-binding riboswitch to establish a gene expression system for conditional tc-dependent control of pre-mRNA ..
  78. pmc Tetracycline-regulatable system to tightly control gene expression in the pathogenic fungus Candida albicans
    H Nakayama
    Department of Mycology, Nippon Roche K K Research Center, 200 Kajiwara, Kamakura, Kanagawa 247 8530, Japan
    Infect Immun 68:6712-9. 2000
    ..To this end, we developed a convenient system to control gene expression in C. albicans by the tetracycline-regulatable (TR) promoters...
  79. ncbi Second-generation tetracycline-regulatable promoter: repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness
    Siamak Agha-Mohammadi
    Hillman Cancer Center, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA
    J Gene Med 6:817-28. 2004
    The tetracycline-regulatable system is one of the most valuable tools for controlling gene expression...
  80. ncbi Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells
    Jullian Jones
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Biotechnol Bioeng 91:722-32. 2005
    ..The level of transferrin produced was found to increase proportionately with tetracycline concentration between 0 and 1 mug/mL with no significant increases in transferrin production above 1 mug/mL...
  81. ncbi Escherichia coli adapts to tetracycline resistance plasmid (pBR322) by mutating endogenous potassium transport: in silico hypothesis testing
    Ferdi L Hellweger
    Department of Civil and Environmental Engineering, Northeastern University, Boston, MA, USA
    FEMS Microbiol Ecol 83:622-31. 2013
    ..Escherichia coli can adapt to the plasmid pBR322 carrying the tetA tetracycline-resistance gene (codes for the TetA efflux pump) by a chromosome mutation, which requires an intact tetA gene ..
  82. ncbi Tetracycline therapy: update
    Marilyn C Roberts
    Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195 7238, USA
    Clin Infect Dis 36:462-7. 2003
    ..Bacterial resistance to tetracycline was identified shortly after the introduction of therapy...
  83. ncbi Effects of minocycline and tetracycline on retinal ganglion cell survival after axotomy
    D C Baptiste
    Laboratory for Retina and Optic Nerve Research, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7
    Neuroscience 134:575-82. 2005
    In the present study, we compared the in vivo neuroprotective efficacy of intraperitoneally administered tetracycline and minocycline to enhance the survival of retinal ganglion cells (RGCs) following unilateral axotomy of the adult rat ..
  84. ncbi Characterization of tetracycline resistance mediated by the efflux pump Tap from Mycobacterium fortuitum
    Santiago Ramón-García
    Departamento de Microbiologia, Medicina Preventiva y Salud Pública, Universidad de Zaragoza, 50009 Zaragoza, Spain
    J Antimicrob Chemother 57:252-9. 2006
    ..fortuitum, to test its sensitivity to well known efflux inhibitors, to study the interaction between tetracycline and these compounds and to test the ability of these compounds to overcome efflux pump-mediated tetracycline ..
  85. pmc Variability of inducible expression across the hematopoietic system of tetracycline transactivator transgenic mice
    Megumi Takiguchi
    Molecular Medicine Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    PLoS ONE 8:e54009. 2013
    The tetracycline (tet)-regulated expression system allows for the inducible overexpression of protein-coding genes, or inducible gene knockdown based on expression of short hairpin RNAs (shRNAs)...
  86. ncbi Tetracycline sorption to coal and soil humic acids: an examination of humic structural heterogeneity
    Huiyu Sun
    State Key Laboratory of Pollution Control and Resource Reuse, and School of the Environment, Nanjing University, Jiangsu 210093, People s Republic of China
    Environ Toxicol Chem 29:1934-42. 2010
    The sorption properties of tetracycline were compared between a coal humic acid (CHA) and a soil humic acid (SHA) under various solution chemistry conditions...
  87. ncbi Vav promoter-tTA conditional transgene expression system for hematopoietic cells drives high level expression in developing B and T cells
    Won Il Kim
    Graduate Program in Molecular, Cellular, Developmental Biology and Genetics, University of Minnesota, Minneapolis, MN 55455, USA
    Exp Hematol 35:1231-9. 2007
    We previously showed that Vav promoter-tetracycline transactivator (Vav-tTA)-driven tetracycline-regulated element (TRE)-NRAS(V12) expression resulted in mastocytosis development in mice...
  88. ncbi Ca2+ and Mg2+ bind tetracycline with distinct stoichiometries and linked deprotonation
    Lihua Jin
    Department of Chemistry, DePaul University, Chicago, IL 60614, USA
    Biophys Chem 128:185-96. 2007
    b>Tetracycline depends on divalent metal ions for its biological function, but its multiple ionization states, conformations, and tautomers at varying solution conditions complicate its ion-binding equilibria, and the stoichiometry of the ..
  89. ncbi Simultaneous adsorption and desorption of cadmium and tetracycline on cinnamon soil
    Ying Wan
    Key Laboratory of Pollution Processes and Environmental Criteria Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
    Chemosphere 80:807-12. 2010
    ..This study aims at evaluating interaction of cadmium (Cd) and a widely-used veterinary antibiotic tetracycline (TC) on the basis of their adsorption and desorption on cinnamon soil using the batch experiments...
  90. ncbi Sorption of tetracycline and chlortetracycline on K- and Ca-saturated soil clays, humic substances, and clay-humic complexes
    Jutta R V Pils
    Agronomy Department, Iowa State University, 100 Osborne Drive, Ames, Iowa 50011, USA
    Environ Sci Technol 41:1928-33. 2007
    b>Tetracycline (TC) and chlortetracycline (CTC) are used extensively for growth promotion and therapeutic purposes in livestock production...
  91. pmc Macrolide and tetracycline resistance and molecular relationships of clinical strains of Streptococcus agalactiae
    Esther Culebras
    Department of Clinical Microbiology, Hospital Universitario San Carlos, 28040 Madrid, Spain
    Antimicrob Agents Chemother 46:1574-6. 2002
    Mechanisms for tetracycline and macrolide resistance in 54 isolates of erythromycin-resistant Streptococcus agalactiae were analyzed by PCR...
  92. ncbi Genes encoding tetracycline resistance in a full-scale municipal wastewater treatment plant investigated during one year
    Stefan Borjesson
    Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Linkoping University, SE 581 85, Linkoping, Sweden
    J Water Health 8:247-56. 2010
    b>Tetracycline-resistant bacteria and genes encoding tetracycline resistance are common in anthropogenic environments...
  93. ncbi Selection of tetracycline inducible self-cleaving ribozymes as synthetic devices for gene regulation in yeast
    Alexander Wittmann
    Institute for Molecular Biosciences, Goethe Universitat Frankfurt am Main, Frankfurt am Main, Germany
    Mol Biosyst 7:2419-27. 2011
    ..Here we describe the selection of tetracycline inducible hammerhead ribozymes...
  94. ncbi Induction of vesicular steatosis by amiodarone and tetracycline is associated with up-regulation of lipogenic genes in HepaRG cells
    Sebastien Antherieu
    Inserm UMR 991, Rennes, France
    Hepatology 53:1895-905. 2011
    ..Many drugs such as amiodarone and tetracycline have been reported to cause phospholipidosis and/or steatosis...
  95. ncbi Sorption and desorption of antibiotic tetracycline on marine sediments
    Xiang Rong Xu
    Environmental Engineering Research Centre, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Chemosphere 78:430-6. 2010
    b>Tetracycline is commonly used for human therapy and veterinary purposes as well as agricultural feed additives. In this study, batch experiments were carried out to investigate the sorption behaviour of tetracycline on marine sediments...
  96. ncbi Water remediation by micelle-clay system: case study for tetracycline and sulfonamide antibiotics
    Tamara Polubesova
    Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
    Water Res 40:2369-74. 2006
    Removal of tetracycline and sulfonamide antibiotics from water by micelles pre-adsorbed on montmorillonite was studied. Micelles of benzyldimethylhexadecylammonium (BDMHDA) were used...
  97. ncbi Helicobacter pylori eradication with a capsule containing bismuth subcitrate potassium, metronidazole, and tetracycline given with omeprazole versus clarithromycin-based triple therapy: a randomised, open-label, non-inferiority, phase 3 trial
    Peter Malfertheiner
    Gastroenterologie, Hepatologie und Infektiologie, Otto von Guericke Universtität, Magdeburg, Germany
    Lancet 377:905-13. 2011
    ..Our aim was to assess the efficacy and safety of a new, single-capsule treatment versus the gold standard for H pylori eradication...
  98. pmc Molecular analysis of tet(W) gene-mediated tetracycline resistance in dominant intestinal Bifidobacterium species from healthy humans
    Ana Belén Flórez
    Instituto de Productos Lácteos de Asturias CSIC, Carretera de Infiesto s n, 33300 Villaviciosa, Asturias, Spain
    Appl Environ Microbiol 72:7377-9. 2006
    tet(W) was found responsible for tetracycline resistance (MICs, 4 to > or =32 microg ml(-1)) in dominant bifidobacterial species from the gastrointestinal tracts of healthy humans...
  99. doi Transposon-mediated stable integration and tetracycline-inducible expression of electroporated transgenes in chicken embryos
    Yoshiko Takahashi
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara 630 0192, Japan
    Methods Cell Biol 87:271-80. 2008
  100. ncbi Use of the tetracycline-controlled transcriptional silencer (tTS) to eliminate transgene leak in inducible overexpression transgenic mice
    Z Zhu
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8057, USA
    J Biol Chem 276:25222-9. 2001
    The doxycycline-inducible reverse tetracycline transactivator (rtTA) is frequently used to overexpress transgenes in a temporally regulated fashion in vivo...
  101. ncbi Tetracycline-inducible transgene expression mediated by a single AAV vector
    A Chtarto
    Interdisciplinary Research Institute, ULB Hospital Erasme, Brussels, Belgium
    Gene Ther 10:84-94. 2003
    ..We describe a single AAV vector in which transcription of both the reverse tetracycline transactivator (rtTA) and the transgene is initiated from a bidirectional tetracycline-responsive promoter and ..

Research Grants74

  1. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..will be performed using three unique NB cell lines, in which MYCN expression can be switched on/off by adding tetracycline. In Specific Aim 2 we will examine the role of polyamines in cell cycle regulation and PI3K/Akt cell survival ..
  2. Mechanism-Based Discovery of Protectants Against Iron-Dependant Oxidative Injury
    JUSTIN M SCHWARTZ; Fiscal Year: 2011
    ..To achieve this, two specific aims are proposed: 1. Screening of tetracycline-derived compounds to test the hypothesis that cytoprotective compounds are blockers of the mitochondrial ..
  3. Inducible recombination and gene expression in specific neurotransmitter systems
    Steven A Thomas; Fiscal Year: 2012
    ..For this approach, the tetracycline-inducible transactivator (tTA2) and reverse tTA (rtTA2S-M2) will be expressed specifically in each ..
  4. CTGF promotes beta cell proliferation &revascularization in islet transplants
    Uma Gunasekaran; Fiscal Year: 2011
    ..Transgenic mice with tetracycline operator-driven CTGF cDNA expression (TetO- CTGF) were interbred with mice in which the rat insulin promoter (..
  5. FEASIBILITY OF REPAIRING GBM DEFECTS IN VIVO
    Jeffrey H Miner; Fiscal Year: 2012
    ..We will use newly generated podocyte-specific reverse tetracycline transactivator transgenic mice and existing collagen IV and laminin mutant and transgenic mice to attempt to ..
  6. Genetic and Optogenetic Models to Dissect the Role of the Serotonin 1B Receptor
    Katherine M Nautiyal; Fiscal Year: 2013
    ..We have therefore generated a mouse line that permits temporal and spatial regulation of 5-HT1B R. A tetracycline operator (tetO) has been inserted between the promoter and coding region of the 5-HT1B R (tetO1B)...
  7. Catalytic inactivation of miRNA function by customized RNase P-based ribozymes
    DANIEL R contact SCHOENBERG; Fiscal Year: 2010
    ..To control the level of ribozyme, it will be expressed from a tetracycline-regulated promoter...
  8. Role of ROS-Induced DNA Damage in Mitochondria-Regulated Apoptosis
    Anne Tann; Fiscal Year: 2012
    ..Also available for this project is a new tetracycline-regulated APE1 siRNA expressing HeLa cell line...
  9. Gonococcal Mechanisms to Evade Host Defenses
    WILLIAM MAURICE SHAFER; Fiscal Year: 2013
    ..g., penicillin and tetracycline) has necessitated the use of more expensive drugs...
  10. Regulators of Cancer-Specific DNA Hypermethylation.
    Jean Pierre J Issa; Fiscal Year: 2013
    ..inserted in specific genomic loci, and later on moved in and out of repressive contexts due to the presence of tetracycline-induced repressors...
  11. miR132 regulation of neuronal plasticity, learning, and memory
    KATELIN L F HANSEN; Fiscal Year: 2013
    ..In Aim 2, I will employ the noted tetracycline-regulated transgenic mouse strain (tTA:miR132) that expresses miR132 in forebrain neurons to explore the ..
  12. The Biosynthesis of Anandamide
    Dale G Deutsch; Fiscal Year: 2010
    ..These mice will also be transgenic for a Cre recombinase gene that is under the control of a tetracycline operon that can be turned on in the absence of doxycycline...
  13. Mathematical Modeling of Inflammation in ARDS
    Gary F Nieman; Fiscal Year: 2010
    ..will enable us to isolate key therapeutic targets and to test novel therapeutics;one such agent is the modified tetracycline COL-3...
  14. Mathematical Modeling of Inflammation in ARDS
    Yoram Vodovotz; Fiscal Year: 2010
    ..will enable us to isolate key therapeutic targets and to test novel therapeutics;one such agent is the modified tetracycline COL-3...
  15. Neuron-specific block of T-type calcium channels
    Edward Perez-Reyes; Fiscal Year: 2012
    ..This will be accomplished using a newly developed version of the tetracycline repressor, which requires drug for activation (doxycycline-ON)...
  16. Role of an RNA-Binding Protein in Mammary Carcinogenesis
    Jeffrey Ross; Fiscal Year: 2009
    ..Aim I exploits a mouse model in which mammary- specific CRD-BP expression is induced by doxycycline (dox), a tetracycline analog. Mammary tumors are expected to arise in dox-treated mice...
  17. Synthesis of bone-selective osteogenic oxysterols
    Frank Stappenbeck; Fiscal Year: 2013
    ..Our strategy involves conjugation of OXY133 to a known bone-targeting agent (BTA) derived from tetracycline. We predict that systemic dosing of such conjugates will result in their selective deposition in bone followed ..
  18. A Genetic Model of Perinatal Hippocampal Iron Deficiency
    Michael Georgieff; Fiscal Year: 2009
    ..Model 2 uses a doxycycline induced (tetracycline transactivator) dominant-negative (DN) approach to functionally reduce the activity of transferrin receptor-1 (..
  19. Molecular genetic control of the immature female mosquito vector
    Helen Benes; Fiscal Year: 2012
    ..We will generate transgenic Ae. aegypti carrying 1) the tetracycline-repressible transcriptional activator gene (tTAV) under control of the Hex-Enh and 2) the Michelob_x gene as an ..
  20. DNA Methyltransferase Target Sites in Cancer
    ROCHELLE LEE TIEDEMANN; Fiscal Year: 2013
    ..cell lines that have been modified to inducibly express DNMT1 and DNMT3B, respectively in the presence of tetracycline. DNA methylation (bisulfite genomic sequencing), histone modifications (ChIP-quantitative PCR) and gene ..
  21. Transgenic Mouse Models of FUS/TLS-Mediated Amyotrophic Lateral Sclerosis
    Zuoshang Xu; Fiscal Year: 2010
    ..third approach, we will express wild type and mutant FUS/TLS in transgenic mice under temporal control using a tetracycline-inducible strategy...
  22. Immunological Mechanisms in Systemic Autoimmune Disease
    Ann Marshak-Rothstein; Fiscal Year: 2012
    ..will involve donor DO11 T cells, skewed to a Th1, Th2, or Th17 phenotype, and recipient mice that inherit (a) a tetracycline transactivator driven by either an invariant chain promoter or a vascular endothelial promoter and (2) a ..
  23. Estrogen signaling transgenic mouse models
    Richard R Behringer; Fiscal Year: 2011
    ..doxycycline-inducible over-expression of ERa was useful, but hindered by the small number of tissue-specific tetracycline transactivator transgenic mouse lines...
  24. Alteration of circadian rhythm and sleep by HIV protein Tat
    Jian M Ding; Fiscal Year: 2012
    ..To assess the long term effect of Tat to the sleep and circadian timing system, we used the tetracycline-dependent and brain (GFAP) specific Tat transgenic mice...
  25. The Regulation of Tumor Progression by Endothelial HoxA5
    NANCY JOAN BOUDREAU; Fiscal Year: 2013
    ..mouse model in which expression of HoxA5 is largely restricted to EC through TIE-2 driven expression of a tetracycline activator protein and HoxA5 can be activated in angiogenic conditions by removal of tetracycline...
  26. Drug and Viral Induced Thyroiditis and Diabetes
    Yaron Tomer; Fiscal Year: 2013
    ..to IFNa;we will also evaluate epigenetic effects in the thyroid in vivo by creating a new transgenic mouse with tetracycline-inducible IFNa expression in the thyroid...
  27. A High-Throughput Model for Human Melanoma
    Sheri L Holmen; Fiscal Year: 2013
    ..Genes that can be targeted therapeutically will be identified by downregulating their expression using a tetracycline-responsive retrovirus or a retrovirus capable of reducing gene expression via RNA interference...
  28. Recombination and Genetics in Chlamydia spp.
    Daniel D Rockey; Fiscal Year: 2010
    ..This includes measurable and frequent in vitro transfer of a tetracycline resistance marker to C. trachomatis from the pig pathogen C. suis...
  29. Remote Inflammation and Atherothrombosis
    NICOLE LEANNE WARD; Fiscal Year: 2013
    ..To determine if remote inflammation can instigate co-morbid complications such as CVD, we engineered a tetracycline-repressible binary mouse model of chronic inflammation by ectopically expressing the angiopoeitin receptor, ..
  30. Epigenetic Changes in Retinoid Signaling in Oral Cavity Carcinogenesis
    KATARZYNA MARIA MARCINKIEWICZ; Fiscal Year: 2013
    ..For Aim 2, I will use tetracycline (doxycycline) regulated vectors for the overexpression/RNAi knockdown experiments...
  31. A Novel Role of the Aryl Hydrocarbon Receptor in Hepatic Steatosis
    Wen Xie; Fiscal Year: 2013
    ..The tetracycline inducible AhR transgenic mice, exhibiting fatty liver even when maintained in chow diet, represent a novel, ..
  32. Role of macrophages in MSC-mediated recovery of hematopoiesis after irradiation
    Manoj M Pillai; Fiscal Year: 2010
    ..To address this limitation, a macrophage-specific, tetracycline-inducible transgenic mouse (CD68-rtTA) has been created and characterized...
  33. Calcium-Stimulated Adenylyl Cyclase Activity's Role in Stress-Facilitated Memory
    LINDSAY ANN WIECZOREK; Fiscal Year: 2010
    ..Through use of a novel transgenic mouse model, which uses a tetracycline-inducible system to replace AC8 expression in AC1 and AC8 double knock-out mice, we are able to assess the time-..
  34. Isolation of a cervical cancer tumor suppressor gene
    Eri S Srivatsan; Fiscal Year: 2012
    ..of cathepsin L, 3) determine the mechanism of cystatin E/M degradation by the HPV 16 E7 protein, 4) use of tetracycline inducible vector system to study the tumor suppressor function of cystatin E/M, and 5) determine the ..
  35. Structural basis of tetracycline resistance by efflux pump TetL
    DANENG WANG; Fiscal Year: 2013
    ..tetracyclines is mediated by membrane transporter proteins that catalyze efflux of tetracyclines in the form of tetracycline-magnesium complex. The structural basis for substrate recognition by such efflux proteins is poorly understood...
  36. Role of Twisted Gastrulation in Osteoclastogenesis
    Rajaram Gopalakrishnan; Fiscal Year: 2013
    ..b>Tetracycline labeling studies showed no significant decrease in mineral apposition rate in Twsg1-null mice compared to WT ..
  37. Macrophage-mediated gene delivery of neurotrophic factors for Parkinson's disease
    Senlin Li; Fiscal Year: 2013
    ..In the chronic toxin model, we will utilize a tetracycline-regulatable (Tet-on) system and address efficacy of our approach by doxycycline-induced switching on of GDNF ..
  38. Modeling Schizophrenia with Altered Expression of Neuregulin 1 (Nrg1)
    NANCY MEIYUN CHIU BIVENS; Fiscal Year: 2013
    ..MicroRNA technology will be utilized with control both by Tetracycline responsive promoters and by Cre recombinase activation...
  39. Defining and demystifying bone quality in type 2 diabetes mellitus
    KENDALL MOSELEY; Fiscal Year: 2013
    ..b>Tetracycline-labeled transiliac bone biopsies will be performed in recruited postmenopausal women in the three categories of ..
  40. Exploring Kabuki Syndrome as a Treatable Cause of Intellectual Disability
    Hans Tomas Bjornsson; Fiscal Year: 2013
    ..Here we propose to create a tetracycline responsive conditional mouse model of Kabuki syndrome...
  41. Estrogen signaling transgenic mouse models
    Richard R Behringer; Fiscal Year: 2012
    ..doxycycline-inducible over-expression of ERa was useful, but hindered by the small number of tissue-specific tetracycline transactivator transgenic mouse lines...
  42. Development and Plasticity of a Retinotectal System
    Mu ming Poo; Fiscal Year: 2012
    ..or mutated forms of Ephrin-B1 or EphB2 in a few RGCs or tectal cells by electroporation, and (3) global tetracycline-induced transgenic expression of these proteins in either all presynaptic RGCs or all postsynaptic tectal cells...
  43. Molecular analysis of oriT-independent transfer in the gonococcal R-plasmids
    LUIS TORRES-BAUZA; Fiscal Year: 2012
    ..of strains of Neisseria gonorrhoeae that express clinically significant levels of resistance to penicillin and tetracycline is a global problem. Three beta-lactamase-encoding (R) plasmids of 7.4 kb, 5.6 kb and 5...
  44. Development of a Mouse Trigeminal Pain Model
    ANDREW RUSSO; Fiscal Year: 2009
    ..Combined with the use of a tetracycline regulated promoter system, this approach will allow comparatively localized and acute expression that should ..
  45. Tetracycline derivatives as SMN2 mRNA splicing modifiers for treatment of SMA.
    S Ken Tanaka; Fiscal Year: 2013
    ..Paratek Pharmaceuticals proposes to develop a novel tetracycline (TC) compound, which has structural similarity to the anthracycline class, to provide a safe, effective drug ..
  46. Developmental Origins of Decreased Resilience
    Rodney W Johnson; Fiscal Year: 2013
    ..We propose a clinically relevant scheme using minocycline, a second-generation tetracycline that inhibits neuroinflammation by blocking activation of microglia.
  47. Synthetic lethality of bicyclomycin
    KARL A DRLICA; Fiscal Year: 2013
    ..g. bacteriostatic concentrations of chloramphenicol or tetracycline) either generates (E. coli) or dramatically increases (K. pneumoniae, A...
  48. Defining the role of BRAF in glioma initiation and maintenance
    Sheri L Holmen; Fiscal Year: 2013
    ..in the natural environment of mammalian physiology and we will use this approach in combination with the tetracycline-regulated expression system to determine if BRAFV600E is required for the maintenance of gliomas in vivo in the ..
  49. The role of endothelial Notch signaling in murine lung branching morphogenesis
    Eric Jelin; Fiscal Year: 2010
    ..In aim 1, we will express a constitutively-active form of Notch4 specifically in the endothelium under tetracycline regulation for variable intervals during lung development in mouse embryos...
  50. MOUSE MODELS OF HUMAN BREAST CANCER
    Jeffrey Rosen; Fiscal Year: 2009
    ..model to functionally assess oncogenesis in mammary gland Sca-1+ progenitor cells by a knock-in of the reverse tetracycline-responsive transactivator (rtTA) and the avian leukosis virus TVA receptor into the Sca-1 locus...
  51. Heart Failure and Thyroid Hormone
    Wolfgang H Dillmann; Fiscal Year: 2010
    ..These effects will be confirmed in binary transgenic mice allowing for tetracycline system based expression of TR in CM...
  52. Acute and Chronic Regulation of 20S Proteasome Function by cAMP-Dependent Kinase
    SARAH SCRUGGS; Fiscal Year: 2012
    ..Transgenic mice expressing a tetracycline-repressible, proteolytically-activating 20S mutation will be generated and subjected to transverse aortic ..
  53. Bmal1 as a Central and Peripheral Regulator of Sleep Homeostasis
    ALLISON JOY BRAGER; Fiscal Year: 2013
    ..Use of the tetracycline transactivator system in these mice also permits for temporal knockdown or rescue of tissue-specific BMAL1 ..
  54. NEUROPEPTIDERGIC INHIBITION OF SPINAL PAIN TRANSMISSION
    Bradley K Taylor; Fiscal Year: 2013
    ..to chronic pain, Aim #1 will utilize transgenic mice (NPYtet) that contain a doxycycline-regulated cassette (tetracycline trans-activator, tTA) at the promoter region of the npy locus...
  55. Disinfectant-resistant mycobacteria
    Mary Jackson; Fiscal Year: 2013
    ..resistance to a number of drugs including rifampicin, ciprofloxacin, clarithromycin, erythromycin, vancomycin, tetracycline and linezolid. Further, our preliminary results indicate that, compared to a GTA-susceptible reference M...
  56. Genetic Analysis of EGFRvIII-independent Glioblastoma Maintenance
    TIFFANY ELIZABETH TAYLOR; Fiscal Year: 2013
    ..Using a genetic model of tetracycline-regulated EGFR expression, the lab previously demonstrated that this receptor is esential for the maintenance ..
  57. CAPSULE REGULATION AND VIRULENCE IN CRYPTOCOCCUS NEOFORMANS
    Tamara L Doering; Fiscal Year: 2013
    ..expression analysis, microscopy, automated network reconstruction, quantitative modeling, RNA interference, tetracycline regulation, and chromatin immunoprecipitation coupled with sequencing...
  58. Trial of Minocycline to Reduce Pain after A1 Pulley and Carpal Tunnel Release
    CATHERINE MILLS CURTIN; Fiscal Year: 2012
    ..The drug to be studied will be minocycline, a tetracycline antibiotic that is a glial cell inhibitor and in animal models, minocycline has shown promise inhibiting ..
  59. LYMPHOTOXIN AND LYMPHOID NEOGENESIS
    Nancy H Ruddle; Fiscal Year: 2010
    ..to maintain HEVs and LVs in TLOs by treatment with LTbR and TNFR inhibitors and by turning LT on and off with a tetracycline inducible system...
  60. Therapeutic potential of ARE-mediated gene expression in Huntington's disease
    KARI RENE HOYT; Fiscal Year: 2010
    ..generated a novel transgenic mouse strain where the are transcription factors Nrf2 and MafK are driven in a tetracycline-inducible manner within forebrain neurons or glia...
  61. Transgenic in vivo delivery of MDA7/IL24 for maligannt glioma therapy
    Isabelle M Germano; Fiscal Year: 2012
    ..that we can generate a pure population of transgenic ESC-derived astrocytes with a highly regulated, robust tetracycline-inducible expression of transgenes...
  62. Isoform Specific Functions for AKt1 and Akt2 in Breast Cancer Progression
    MARIA LYNN MANCINI; Fiscal Year: 2010
    ..We will utilize a tissue specific tetracycline regulated siRNA transgene system to modulate expression in a dose dependent manner of the Akt1 and 2 in a tumor ..
  63. TRANSCRIPTIONAL CONTROL OF MAMMALIAN BRAIN DEVELOPMENT
    Eseng Lai; Fiscal Year: 2001
    ..differentiation, the PI has shown, using a mink lung cell line where BF-1 expression is under control of tetracycline withdrawal, that the presence of BF-1 promotes entry into S phase in the presence of TGF-b...
  64. Peptide Derivatives to treat Urinary Tract Infections
    Laszlo Otvos; Fiscal Year: 2003
    ..Pyrrhocoricin and its designed analogs kill (-lactam-, tetracycline- and aminoglycoside-resistant Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium, and Haemophilus ..
  65. NUCLEIC ACID PROBES OF RIBOSOMAL STRUCTURE AND FUNCTION
    BARRY COOPERMAN; Fiscal Year: 2003
    ..Many antibiotics in current clinical use, such as tetracycline, erythromycin and other macrolides, neomycin and other aminoglycosides, and chloramphenicol target ribosomes as ..
  66. Enhancement of antimicrobics by electrical current
    Robin Patel; Fiscal Year: 2007
    ..of electric current can enhance the killing of biofilm bacteria by non-aminoglycoside, non-quinolone, non-tetracycline antimicrobics and whether the bioelectric effect is generalizable to Staphylococcus aureus...
  67. SIRT1 Overexpression in Cellular Mitochondrial Metabolism and Function
    James Russell; Fiscal Year: 2009
    ..that expresses mouse SIRT1 and mitochondrial targeted enhanced yellow fluorescent protein under the control of tetracycline responsive element (TRE-SIRT1/mito-eYFP)...
  68. ANTIBIOTIC SUSCEPTIBILITIES OF PERIODONTAL BACTERIA
    Clay Walker; Fiscal Year: 1993
    ..whose disease condition does not respond to conventional therapy combined with the adjunctive use of tetracycline antibiotics...
  69. Azithromycin Combination Therapy in Resistant Malaria
    Charles Knirsch; Fiscal Year: 2000
    ..problem because the drugs commonly added to the above standard agents to augment efficacy-such as doxycycline/tetracycline or mefloquine-are contraindicated for these populations...
  70. Bone Targeting Peptides for the Prevention and Treatment of Infection
    HANNE GRON; Fiscal Year: 2009
    ..The use of general bone-binding moieties such as bisphosphonates, poly-aspartic acid and tetracycline provides preliminary evidence that localized drug delivery to bone is possible in numerous in vitro studies and ..
  71. Molecular mechanism of immune evasion by Yersinia pestis
    Tomas Mustelin; Fiscal Year: 2003
    ..Although several vaccines exist, and Yersinia usually is sensitive to streptomycin and tetracycline type antibiotics, the pneumonic form of the disease is difficult to treat and still often results in death...
  72. STRUCTURE AND GENETIC CONTROL OF COLICINES
    Donald Helinski; Fiscal Year: 2005
    ..Plasmid RK2 specifies resistance to the antibiotics ampicillin, tetracycline and kanamycin, and will replicate and is stably maintained in a wide range of Gram-negative bacteria...
  73. DEVELOPMENT OF TN916 INTO A MYCOPLASMA GENETIC TOOL
    Kevin Dybvig; Fiscal Year: 1993
    ..Transformed cells can be selected by their expression of the tetracycline resistance determinant, tetM, located on Tn916, Tetracycline-resistant transformants contain Tn916 inserted ..
  74. INTESTINAL LIPOPROTEINS IN THE NEONATE
    Dennis Black; Fiscal Year: 2007
    ..Further studies are proposed using an AAV vector expression system, tetracycline regulatable gene expression technology and subcellular fractionation techniques to test the main hypothesis ..