Summary: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)

Top Publications

  1. ncbi AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR
    R A Laskowski
    Department of Biochemistry and Molecular Biology, University College London, UK
    J Biomol NMR 8:477-86. 1996
  2. pmc Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing
    D I Svergun
    European Molecular Biology Laboratory, Hamburg, Germany and Institute of Crystallography, Russian Academy of Sciences, Moscow, Russia
    Biophys J 76:2879-86. 1999
  3. ncbi Structure of the human telomere in K+ solution: an intramolecular (3 + 1) G-quadruplex scaffold
    Kim Ngoc Luu
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Am Chem Soc 128:9963-70. 2006
  4. pmc Characterizing flexible and intrinsically unstructured biological macromolecules by SAS using the Porod-Debye law
    Robert P Rambo
    Life Sciences Division, Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Biopolymers 95:559-71. 2011
  5. ncbi Improved stability of Drosophila larval neuromuscular preparations in haemolymph-like physiological solutions
    B A Stewart
    Department of Physiology, University of Toronto, Ontario, Canada
    J Comp Physiol A 175:179-91. 1994
  6. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
  7. ncbi Crowding effects on diffusion in solutions and cells
    James A Dix
    Department of Chemistry, State University of New York, Binghamton, New York 13902, USA
    Annu Rev Biophys 37:247-63. 2008
  8. pmc Grid inhomogeneous solvation theory: hydration structure and thermodynamics of the miniature receptor cucurbit[7]uril
    Crystal N Nguyen
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 0736, USA
    J Chem Phys 137:044101. 2012
  9. pmc Not so crystal clear: the structure of the human telomere G-quadruplex in solution differs from that present in a crystal
    Jing Li
    Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216 4505, USA
    Nucleic Acids Res 33:4649-59. 2005
  10. ncbi Applications of small-angle X-ray scattering to biomacromolecular solutions
    Maxim V Petoukhov
    European Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, 22607 Hamburg, Germany
    Int J Biochem Cell Biol 45:429-37. 2013

Detail Information

Publications387 found, 100 shown here

  1. ncbi AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR
    R A Laskowski
    Department of Biochemistry and Molecular Biology, University College London, UK
    J Biomol NMR 8:477-86. 1996
    ..They are intended to be of use both to support ongoing NMR structure determination and in the validation of the final results...
  2. pmc Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing
    D I Svergun
    European Molecular Biology Laboratory, Hamburg, Germany and Institute of Crystallography, Russian Academy of Sciences, Moscow, Russia
    Biophys J 76:2879-86. 1999
    ..Application of the method is illustrated by the restoration of a ribosome-like model structure and more realistically by the determination of the shape of several proteins from experimental x-ray scattering data...
  3. ncbi Structure of the human telomere in K+ solution: an intramolecular (3 + 1) G-quadruplex scaffold
    Kim Ngoc Luu
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Am Chem Soc 128:9963-70. 2006
    ..The topological characteristics of this (3 + 1) G-quadruplex scaffold should provide a unique platform for structure-based anticancer drug design targeted to human telomeric DNA...
  4. pmc Characterizing flexible and intrinsically unstructured biological macromolecules by SAS using the Porod-Debye law
    Robert P Rambo
    Life Sciences Division, Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Biopolymers 95:559-71. 2011
  5. ncbi Improved stability of Drosophila larval neuromuscular preparations in haemolymph-like physiological solutions
    B A Stewart
    Department of Physiology, University of Toronto, Ontario, Canada
    J Comp Physiol A 175:179-91. 1994
    ..preparations from third instar larvae of Drosophila are not well-maintained in commonly used physiological solutions: vacuoles form in the muscle fibers, and membrane potential declines...
  6. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
    ..This new type of data analysis has a broad application in biology and it provides a powerful tool for measuring fast as well as slower dynamic processes in cellular systems using any standard laser confocal microscope...
  7. ncbi Crowding effects on diffusion in solutions and cells
    James A Dix
    Department of Chemistry, State University of New York, Binghamton, New York 13902, USA
    Annu Rev Biophys 37:247-63. 2008
    ..Experimental measurements of solute diffusion in solutions and cellular aqueous compartments, however, generally show Brownian diffusion...
  8. pmc Grid inhomogeneous solvation theory: hydration structure and thermodynamics of the miniature receptor cucurbit[7]uril
    Crystal N Nguyen
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 0736, USA
    J Chem Phys 137:044101. 2012
    ..Thus, localized regions of high solvent density can be generated in a binding site without strong, attractive solute-solvent interactions...
  9. pmc Not so crystal clear: the structure of the human telomere G-quadruplex in solution differs from that present in a crystal
    Jing Li
    Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216 4505, USA
    Nucleic Acids Res 33:4649-59. 2005
    ..that are inconsistent with the reported crystal structure, indicating that a different structure exists in K+ solutions. Sedimentation coefficients were determined experimentally in both Na+ and K+ solutions and were compared with ..
  10. ncbi Applications of small-angle X-ray scattering to biomacromolecular solutions
    Maxim V Petoukhov
    European Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, 22607 Hamburg, Germany
    Int J Biochem Cell Biol 45:429-37. 2013
    ..for SAXS data analysis are presented in this review including rapid characterization of macromolecular solutions in terms of low-resolution shapes, validation of high-resolution models in close-to-native conditions, ..
  11. pmc An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human BCL-2 promoter region in solution
    Jixun Dai
    College of Pharmacy, University of Arizona, Tucson, 85721, USA
    J Am Chem Soc 128:1096-8. 2006
    ..This predominant bcl-2 G-quadruplex represents an attractive novel target for the design of new anticancer drugs that specifically modulate bcl-2 gene expression...
  12. ncbi Structural characterization of proteins and complexes using small-angle X-ray solution scattering
    Haydyn D T Mertens
    European Molecular Biology Laboratory Hamburg Outstation, c o DESY, Notkestrasse 85, Hamburg, Germany
    J Struct Biol 172:128-41. 2010
    ..analysis methods have reached an advanced state, allowing for automated and rapid characterization of protein solutions in terms of low-resolution models, quaternary structure and oligomeric composition...
  13. ncbi On the mechanism of hydrolysis of phosphate monoesters dianions in solutions and proteins
    Marco Klahn
    Department of Chemistry, University of Southern California, Los Angeles, California 90089 1062, USA
    J Am Chem Soc 128:15310-23. 2006
    The nature of the hydrolysis of phosphate monoester dianions in solutions and in proteins is a problem of significant current interest...
  14. pmc Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution
    Attila Ambrus
    College of Pharmacy, The University of Arizona, 1703 E Mabel St, Tucson, AZ 85721, USA
    Nucleic Acids Res 34:2723-35. 2006
  15. pmc Multidomain assembled states of Hck tyrosine kinase in solution
    Sichun Yang
    Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 107:15757-62. 2010
    ..Here, BSS-SAXS reconstruction is used to reveal the structural organization of Hck in solution and the different shifts in the equilibrium population of assembly states upon the binding of different signaling peptides...
  16. ncbi Some precautions in using chelators to buffer metals in biological solutions
    Chris Patton
    Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950, USA
    Cell Calcium 35:427-31. 2004
    ..This communication discusses common misunderstandings and pitfalls in use of these buffers and provides information on choosing the best metal buffer for different experimental situations...
  17. pmc Method for determining molar concentrations of metabolites in complex solutions from two-dimensional 1H-13C NMR spectra
    Ian A Lewis
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Anal Chem 79:9385-90. 2007
    ..2% technical error under nearly ideal conditions. We propose FMQ by NMR as a practical alternative to 1D (1)H NMR for metabolomics studies in which 50-mg (extract dry weight) samples can be obtained...
  18. ncbi Effect of aggregation kinetics on the thermal conductivity of nanoscale colloidal solutions (nanofluid)
    Ravi Prasher
    Intel Corporation, Chandler, Arizona 85226 3699, USA
    Nano Lett 6:1529-34. 2006
    ..of k with respect to nanoparticle size, an increase as well as a decrease in the ratio of k of these colloidal solutions with the k of the base fluid with increasing temperature, and a dependence of k on pH and time...
  19. ncbi Classification with correlated features: unreliability of feature ranking and solutions
    Laura Tolosi
    Department of Computational Biology and Applied Algorithmics, Max Planck Institute for Informatics, Saarbrucken, Germany
    Bioinformatics 27:1986-94. 2011
  20. ncbi Solution structure and model membrane interactions of temporins-SH, antimicrobial peptides from amphibian skin. A NMR spectroscopy and differential scanning calorimetry study
    Feten Abbassi
    UPMC Univ Paris 06, CNRS FRE 2852, Peptidome de la Peau des Amphibiens, F 75005 Paris, France
    Biochemistry 47:10513-25. 2008
    ..This suggests that the charge-induced activity of temporins-SH for bacterial cells is due to changes in the membrane-disturbing mechanism of the bound peptides...
  21. pmc Determination of domain structure of proteins from X-ray solution scattering
    D I Svergun
    European Molecular Biology Laboratory, Hamburg Outstation, D 22603 Hamburg, Germany
    Biophys J 80:2946-53. 2001
    ..The new method substantially improves the resolution and reliability of models derived from scattering data and makes solution scattering a useful technique in large-scale structural characterization of proteins...
  22. pmc Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form
    Sheena D'Arcy
    Howard Hughes Medical Institute, Colorado State University, Fort Collins, CO 80523, USA Electronic address
    Mol Cell 51:662-77. 2013
    ..The organization of the complex reveals that Nap1 competes with histone-DNA and interhistone interactions observed in the nucleosome, thereby regulating the availability of histones for chromatin assembly. ..
  23. pmc Superheating of ice crystals in antifreeze protein solutions
    Yeliz Celik
    Department of Physics and Astronomy, Ohio University, Athens, OH 45701, USA
    Proc Natl Acad Sci U S A 107:5423-8. 2010
    ..Here we present the first quantitative measurements of superheating of ice in AFP solutions. Superheated ice crystals were stable for hours above their equilibrium melting point, and the maximum ..
  24. ncbi Removal of Cr(VI) from polluted solutions by electrocoagulation: Modeling of experimental results using artificial neural network
    S Aber
    Department of Applied Chemistry, Faculty of Chemistry, Environmental Protection Research Laboratory, University of Tabriz, Tabriz, Iran
    J Hazard Mater 171:484-90. 2009
    ..A comparison between the model results and experimental data gave high correlation coefficient (R(2)=0.976) shows that the model is able to predict the concentration of residual Cr(VI) in the solution...
  25. pmc A low frequency electromagnetic sensor for indirect measurement of glucose concentration: in vitro experiments in different conductive solutions
    Andrea Tura
    ISIB CNR, Corso Stati Uniti 4, 35127 Padua, Italy
    Sensors (Basel) 10:5346-58. 2010
    ..sensor, and we tested in vitro its ability to estimate variations in glucose concentration of different solutions with similarities to blood (sodium chloride and Ringer-lactate solutions), differing though in the lack of any ..
  26. pmc The ligand-free state of the TPP riboswitch: a partially folded RNA structure
    Mona Ali
    Department of Applied Physics, Stanford University, Stanford, CA 94305, USA
    J Mol Biol 396:153-65. 2010
    ..Our results also demonstrate the power of combining experimental small-angle X-ray scattering data with theoretical structure prediction tools in the determination of RNA structures beyond riboswitches...
  27. pmc Bridging the solution divide: comprehensive structural analyses of dynamic RNA, DNA, and protein assemblies by small-angle X-ray scattering
    Robert P Rambo
    Life Science Division, Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Curr Opin Struct Biol 20:128-37. 2010
    ..Here, we review recent SAXS computational tools, technologies, and applications to nucleic acids and related structures...
  28. pmc Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation
    P Schuck
    Bioengineering and Physical Science Program, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 75:1503-12. 1998
    ..In addition, in the case of a protein in rapid monomer-dimer equilibrium, this configuration was found to reveal the most precise estimate of the association constant...
  29. ncbi Surface acoustic wave biosensors: a review
    Kerstin Länge
    Institute for Microstructure Technology, Forschungszentrum Karlsruhe, P O Box 3670, 76021, Karlsruhe, Germany
    Anal Bioanal Chem 391:1509-19. 2008
    ..permit the highly sensitive detection of biorelevant molecules in liquid media (such as water or aqueous buffer solutions)...
  30. pmc Affinity makes the difference: nonselective interaction of the UBA domain of Ubiquilin-1 with monomeric ubiquitin and polyubiquitin chains
    Daoning Zhang
    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20910, USA
    J Mol Biol 377:162-80. 2008
    ..The structural data obtained in this study provide insights into the possible structural reasons for the diversity of polyubiquitin chain recognition by UBA domains...
  31. ncbi Solution properties of the galactomannans extracted from the seeds of Caesalpinia pulcherrima and Cassia javanica: comparison with locust bean gum
    C T Andrade
    Instituto de Macromoléculas Professora Eloisa Mano, Universidade Federal do Rio de Janeiro, RJ, Brazil
    Int J Biol Macromol 26:181-5. 1999
    ..The correlation between dynamic and steady shear properties (Cox Merz rule) was satisfactory for the three galactomannans...
  32. ncbi Structure of propeller-type parallel-stranded RNA G-quadruplexes, formed by human telomeric RNA sequences in K+ solution
    Herry Martadinata
    Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371
    J Am Chem Soc 131:2570-8. 2009
    ..Based on these findings we proposed how higher-order structures might be formed by long telomeric RNA...
  33. ncbi Solution structure of the DNA binding domain of rice telomere binding protein RTBP1
    Sunggeon Ko
    Department of Biochemistry and Biology, Protein Network Research Center, Yonsei University, Seoul 120 749, Korea
    Biochemistry 48:827-38. 2009
    ..These results suggest that helix 3 functions as a sequence-specific recognition helix while the N-terminal arm stabilizes the DNA binding...
  34. pmc Triggering germination represents a novel strategy to enhance killing of Clostridium difficile spores
    Michelle M Nerandzic
    Research Service, Cleveland Veterans Affairs Medical Center, Cleveland, Ohio, United States of America
    PLoS ONE 5:e12285. 2010
    ..difficile spores to radiation or other stressors...
  35. ncbi Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein
    Edvards Liepinsh
    Karolinska Institute, , Stockholm, Sweden
    Eur J Biochem 269:1259-66. 2002
    ..The present study presents the first complete sequence-specific resonance assignments and the first complete solution structure determination by NMR of any AFP I protein...
  36. ncbi NMR analysis of a 900K GroEL GroES complex
    Jocelyne Fiaux
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, CH 8093 Zurich, Switzerland
    Nature 418:207-11. 2002
    ..This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes...
  37. pmc Three-dimensional structure and orientation of rat islet amyloid polypeptide protein in a membrane environment by solution NMR spectroscopy
    Ravi Prakash Reddy Nanga
    Department of Chemistry and Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 131:8252-61. 2009
  38. ncbi Semi-rigid solution structures of heparin by constrained X-ray scattering modelling: new insight into heparin-protein complexes
    Sanaullah Khan
    Department of Structural and Molecular Biology, Division of Biosciences, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 395:504-21. 2010
    ..We conclude that heparin in solution has a semi-rigid and extended conformation that is preformed for its optimal binding to protein targets without major conformational changes...
  39. ncbi Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH(*) radical in alcoholic solutions
    Mario C Foti
    Istituto di Chimica Biomolecolare del CNR Sez di Catania, Via del Santuario 110, I 95028 Valverde CT, Italy
    J Org Chem 69:2309-14. 2004
    ..These impurities dramatically influence the ionization equilibrium of phenols and cause a reduction or an enhancement, respectively, of the measured rate constants...
  40. ncbi NMR solution structure of SlyD from Escherichia coli: spatial separation of prolyl isomerase and chaperone function
    Ulrich Weininger
    Institut fur Physik, Fachgruppe Biophysik, Martin Luther Universitat Halle Wittenberg, D 06099 Halle Saale, Germany
    J Mol Biol 387:295-305. 2009
    ..The capacity of the IF domain to establish an initial encounter-collision complex, together with the flexible orientation of the two interacting domains, makes SlyD* a very powerful catalyst of protein folding...
  41. pmc Complex behavior in solution of homodimeric SecA
    Ronald L Woodbury
    School of Molecular Biosciences, Washington State University, Pullman, Washington 99164 4660, USA
    Protein Sci 11:875-82. 2002
  42. ncbi Relationship between varietal amino acid profile of grapes and wine aromatic composition. Experiments with model solutions and chemometric study
    Purificacion Hernandez-Orte
    Department of Analytical Chemistry, Faculty of Sciences, University of Zaragoza, 50009 Zaragoza, Spain
    J Agric Food Chem 50:2891-9. 2002
    Synthetic solutions containing amino acids, sugar, water, and yeast nutrients have been fermented by Saccharomyces cerevisiae, and the volatile composition of the fermented media has been analyzed by GC...
  43. ncbi Molecular recognition in the HIV-1 capsid/cyclophilin A complex
    S Yoo
    Department of Biochemistry, University of Utah, Salt Lake City 84132, USA
    J Mol Biol 269:780-95. 1997
    ..These studies reveal that the active site of CypA, which can catalyze the isomerization of proline residues in vitro, also functions as a sequence-specific, protein-binding motif in HIV-1 replication...
  44. ncbi Comparison of reduced volume versus four liters sulfate-free electrolyte lavage solutions for colonoscopy colon cleansing
    Jack A DiPalma
    Division of Gastroenterology, University of South Alabama College of Medicine, Mobile, Alabama 36693, USA
    Am J Gastroenterol 98:2187-91. 2003
    ..o. bisacodyl (Half Lytely, Braintree Laboratories) was compared with standard 4 L SF-ELS lavage for safety and efficacy...
  45. ncbi Equilibrium cluster formation in concentrated protein solutions and colloids
    Anna Stradner
    Department of Physics, University of Fribourg, Chemin du Musee 3, CH 1700 Fribourg, Switzerland
    Nature 432:492-5. 2004
    ..successfully applied to complex soft-matter systems, such as colloid-polymer systems and concentrated protein solutions. However, intriguing problems like the frequent occurrence of cluster phases remain...
  46. pmc Structure of the chromatin binding (chromo) domain from mouse modifier protein 1
    L J Ball
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    EMBO J 16:2473-81. 1997
    ..Structural comparisons suggest that chromo domains, of which more than 40 are now known, act as protein interaction motifs and that the MoMOD1 protein acts as an adaptor mediating interactions between different proteins...
  47. ncbi Solution structures of UBA domains reveal a conserved hydrophobic surface for protein-protein interactions
    Thomas D Mueller
    Department of Chemistry and Biochemistry, Molecular Biology Institute, University of California at Los Angeles, 90095 1569, USA
    J Mol Biol 319:1243-55. 2002
    ..Detailed comparison of the structures of the two UBA domains, combined with previous mutagenesis studies, indicates that the binding site of HIV-1 Vpr on UBA(2) does not completely overlap the ubiquitin binding site...
  48. ncbi NMR studies in aqueous solution fail to identify significant conformational differences between the monomeric forms of two Alzheimer peptides with widely different plaque-competence, A beta(1-40)(ox) and A beta(1-42)(ox)
    R Riek
    , , , Switzerland
    Eur J Biochem 268:5930-6. 2001
    ..The lack of the observation of more extensive conformational differences between the two molecules is intriguing, considering that A beta(1-42)(ox) in aqueous solution has much higher plaque-competence than A beta(1-40)(ox)...
  49. ncbi Removal of methylene blue dye from aqueous solutions by adsorption using yellow passion fruit peel as adsorbent
    Flávio André Pavan
    Instituto de Quimica, Universidade Estadual de Campinas, UNICAMP, PO Box 6154, 13084 971 Campinas, SP, Brazil
    Bioresour Technol 99:3162-5. 2008
    ..tested as an alternative low-cost adsorbent for the removal of a basic dye, methylene blue (MB), from aqueous solutions. Adsorption of MB onto this natural adsorbent was studied by batch adsorption isotherms at room temperature...
  50. ncbi Biosorption of heavy metals and radionuclide from aqueous solutions by pre-treated arca shell biomass
    Sudhir Dahiya
    Environmental Studies Section, Health Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India
    J Hazard Mater 150:376-86. 2008
    ..Arca shell biomass can be effectively and efficiently employed for removal of studied elements after optimisation of parameters...
  51. ncbi Guava (Psidium guajava) leaf powder: novel adsorbent for removal of methylene blue from aqueous solutions
    V Ponnusami
    Department of Chemical Engineering, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur 613402, India
    J Hazard Mater 152:276-86. 2008
    ..out using a novel adsorbent, guava leaf powder (GLP), for the removal of methylene blue (MB) from aqueous solutions. Potential of GLP for adsorption of MB from aqueous solution was found to be excellent...
  52. ncbi The effective hard particle model provides a simple, robust, and broadly applicable description of nonideal behavior in concentrated solutions of bovine serum albumin and other nonassociating proteins
    Allen P Minton
    Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, NIH DHHS, Bethesda, MD 20892 0830, USA
    J Pharm Sci 96:3466-9. 2007
    Published data on the concentration dependence of osmotic pressure of solutions of bovine serum albumin in 0...
  53. ncbi Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter
    Anh Tuân Phan
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Am Chem Soc 129:4386-92. 2007
    ..This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit...
  54. ncbi A new strategy for structure determination of large proteins in solution without deuteration
    Yingqi Xu
    Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543
    Nat Methods 3:931-7. 2006
    ..The strategy extends the size limit for structure determination by NMR spectroscopy to 42 kDa for monomeric proteins and to 65 kDa for differentially labeled multimeric proteins without the need for deuteration or selective labeling...
  55. ncbi Optimizing high-throughput metabolomic biomarker screening: a study of quenching solutions to freeze intracellular metabolism in CHO cells
    Jennifer Kronthaler
    University of Innsbruck, Innsbruck, Austria
    OMICS 16:90-7. 2012
    ..Three precooled quenching solutions were tested for their ability to fix the metabolic activity of CHO cells: phosphate-buffered saline (PBS) (pH 7...
  56. pmc Self-assembly and conformational heterogeneity of the AXH domain of ataxin-1: an unusual example of a chameleon fold
    Cesira de Chiara
    Medical Research Council National Institute for Medical Research London, United Kingdom
    Biophys J 104:1304-13. 2013
  57. pmc Adsorption of Cr(VI) and speciation of Cr(VI) and Cr(III) in aqueous solutions using chemically modified chitosan
    Jun Dai
    College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, China
    Int J Environ Res Public Health 9:1757-70. 2012
    ..The detection limit of this method was 20 ng/L, the relatively standard deviation was 1.2% and the recovery was 99%~105%...
  58. pmc Highly robust, recyclable displacement assay for mercuric ions in aqueous solutions and living cells
    Dingbin Liu
    Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu 214063, China
    ACS Nano 6:10999-1008. 2012
    ..Besides, this displacement assay can be regenerated by resupplying free RBITC into the AuNPs solutions that were already used for detecting Hg(2+). Importantly, the detection limit of this assay for Hg(2+) (2...
  59. pmc A statistical-mechanical theory of fibril formation in dilute protein solutions
    Jeroen van Gestel
    Physical Chemistry and Molecular Thermodynamics Group, DelftChemTech, Technische Universiteit Delft, 2628 BL Delft, The Netherlands
    Biophys J 90:3134-45. 2006
    We outline a theoretical treatment that describes fibril formation in dilute protein solutions. For this, we combine a theory describing self-assembly and conformational transition with a description of the lateral association of linear ..
  60. pmc Solution structure of the first Sam domain of Odin and binding studies with the EphA2 receptor
    Flavia Anna Mercurio
    Department of Biological Sciences, University of Naples Federico II, Naples, Italy
    Biochemistry 51:2136-45. 2012
    ..This work further validates structural elements relevant for the heterotypic Sam-Sam interactions of EphA2 and provides novel insights for the design of potential therapeutic compounds that can modulate receptor endocytosis...
  61. ncbi Untreated coffee husks as biosorbents for the removal of heavy metals from aqueous solutions
    Waleska E Oliveira
    Departamento de Engenharia Quimica, UFMG, R Espírito Santo, 35 Sexto Andar, 30160 030 Belo Horizonte, MG, Brazil
    J Hazard Mater 152:1073-81. 2008
    ..husks (CH), a coffee processing residue, as untreated sorbents for the removal of heavy metal ions from aqueous solutions. Biosorption studies were conducted in a batch system as a function of contact time, initial metal ion ..
  62. ncbi Isomerization of spirobenzopyrans bearing electron-donating and electron-withdrawing groups in acidic aqueous solutions
    Taku Satoh
    Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 5, 1 1 1, Higashi, Tsukuba, Ibaraki 305 8568, Japan
    Phys Chem Chem Phys 13:7322-9. 2011
    ..The effects of the substitution can be explained by changes in the electron density of the oxygen atom of the spirobenzopyrans...
  63. ncbi From sulfur-amine solutions to metal sulfide nanocrystals: peering into the oleylamine-sulfur black box
    Jordan W Thomson
    Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario M5S 3H6, Canada
    J Am Chem Soc 133:5036-41. 2011
    In this Article, we present our findings on the formation of metal sulfide nanocrystals from sulfur-alkylamine solutions. By pulsed field gradient diffusion NMR along with the standard toolbox of 1D and 2D NMR, we determined that sulfur-..
  64. ncbi A modified protocol to prepare seed-free starting solutions of amyloid-β (Aβ)₁₋₄₀ and Aβ₁₋₄₂ from the corresponding depsipeptides
    Marten Beeg
    Department of Biochemistry and Molecular Pharmacology, Instituto di Ricerche Farmacologiche Mario Negri, 20156 Milano, Italy
    Anal Biochem 411:297-9. 2011
    Preparing reliable, seed-free stock solutions of the highly amyloidogenic peptides amyloid-β (Aβ) is difficult...
  65. pmc Successful stabilization of graphene oxide in electrolyte solutions: enhancement of biofunctionalization and cellular uptake
    Bong Jin Hong
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    ACS Nano 6:63-73. 2012
    ..or steric stabilization, have been developed to enhance the stability of graphene oxide in electrolyte solutions, allowing it to stay dispersed in cell culture media and serum...
  66. pmc Functional characterization of alternate optimal solutions of Escherichia coli's transcriptional and translational machinery
    Ines Thiele
    Center for Systems Biology, University of Iceland, Reykjavik, Iceland
    Biophys J 98:2072-81. 2010
    ..The subset of alternate optimal solutions (AOS) consistent with maximal ribosome production was calculated...
  67. ncbi Motion analysis of self-propelled Pt-silica particles in hydrogen peroxide solutions
    Hua Ke
    C Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, West Virginia 26506 6045, USA
    J Phys Chem A 114:5462-7. 2010
    Silica microspheres that are half-coated with platinum metal undergo self-propulsion in solutions of H(2)O(2), with the average speed increasing with increasing H(2)O(2) concentration...
  68. ncbi Estimating viral titres in solutions with low viral loads
    C Brownie
    Statistical consultant, Raleigh, NC 27627, USA
    Biologicals 39:224-30. 2011
    ..In this case, the SK method produces upwardly biased estimates of titres. Based on our results, we recommend the use of either ML or SK at most virus concentrations; however, at low virus concentrations ML is preferred...
  69. ncbi Scaling form of viscosity at all length-scales in poly(ethylene glycol) solutions studied by fluorescence correlation spectroscopy and capillary electrophoresis
    Robert Holyst
    Institute of Physical Chemistry PAS, Kasprzaka 44 52, 01 224 Warsaw, Poland
    Phys Chem Chem Phys 11:9025-32. 2009
    ..The SE relation is correct even in PEG solutions of very high viscosity (three orders of magnitude larger than that of water).
  70. ncbi Removal of hexavalent chromium [Cr(VI)] from aqueous solutions by the diatomite-supported/unsupported magnetite nanoparticles
    Peng Yuan
    Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
    J Hazard Mater 173:614-21. 2010
    ..These results indicate that the diatomite-supported/unsupported magnetite nanoparticles are readily prepared, enabling promising applications for the removal of Cr(VI) from aqueous solution...
  71. ncbi Measurement of aptamer-protein interactions with back-scattering interferometry
    Ian R Olmsted
    Anal Chem 83:8867-70. 2011
    ..Preliminary modeling efforts suggest evidence for allosteric linkage between the two exosites...
  72. ncbi Hypothermic storage of isolated human hepatocytes: a comparison between University of Wisconsin solution and a hypothermosol platform
    Alina Ostrowska
    Wroclaw University of Environmental and Life Sciences, Wrocław, Poland
    Arch Toxicol 83:493-502. 2009
    ..Although hepatocytes are shown to be satisfactory after 24 h storage in all of the tested solutions, the cell viability, energy status, and xenobiotic metabolism following cold preservation in HTS-FRS was ..
  73. ncbi Biosorption of cadmium (II) and lead (II) from aqueous solutions using mushrooms: a comparative study
    R Vimala
    School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore, Tamil Nadu, India
    J Hazard Mater 168:376-82. 2009
    ..cadmium (II) and lead (II) from aqueous solutions. The optimum sorption conditions were studied for each metal separately...
  74. ncbi A two-dimensional spectrum analysis for sedimentation velocity experiments of mixtures with heterogeneity in molecular weight and shape
    Emre Brookes
    Department of Biochemistry, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, MC 7760, San Antonio, TX 78229 3901, USA
    Eur Biophys J 39:405-14. 2010
    ..which permits simultaneous determination of shape and molecular weight distributions for mono- and polydisperse solutions of macromolecules...
  75. ncbi Study of the acceptability of antibiotic syrups, suspensions, and oral solutions prescribed to pediatric outpatients
    Robert Cohen
    Laboratoire de Microbiologie, Hopital Intercommunal de Creteil, Creteil, France
    Eur J Pediatr 168:851-7. 2009
    ..Poor compliance increases the risk of therapeutic failures and the emergence of resistance...
  76. ncbi The removal of phenol from aqueous solutions by adsorption using surfactant-modified bentonite and kaolinite
    Uday F Alkaram
    Department of Chemistry, College of Education, Ibn Al Haitham, University of Baghdad, Baghdad, Iraq
    J Hazard Mater 169:324-32. 2009
    ..The ability of raw and organo-modified clays to remove phenol from aqueous solutions has been carried out as a function of contact time, pH and temperatures using a batch technique...
  77. ncbi Removal of copper ions from aqueous solutions by kaolinite and batch design
    Mahir Alkan
    Balikesir University, Faculty of Science and Literature, Department of Chemistry, Cagis Balikesir, Turkey
    J Hazard Mater 153:867-76. 2008
    The removal of copper ions from aqueous solutions by kaolinite was investigated by using a batch-type method...
  78. ncbi Enhanced diffusion of polycyclic aromatic hydrocarbons in artificial and natural aqueous solutions
    Philipp Mayer
    National Environmental Research Institute, University of Aarhus, P O Box 358, 4000 Roskilde, Denmark
    Environ Sci Technol 41:6148-55. 2007 determine the diffusive mass transfer of 12 polycyclic aromatic hydrocarbons through water, air, surfactant solutions, humic acid solutions, aqueous soil and horse manure extracts, digestive fluid of a deposit-feeding worm, and ..
  79. ncbi Adsorption of crude oil from aqueous solution by hydrogel of chitosan based polyacrylamide prepared by radiation induced graft polymerization
    H H Sokker
    Jazan University, Faculty of Science, Saudi Arabia hesham
    J Hazard Mater 190:359-65. 2011
    ..Equilibrium data were found to fit very well with both Freundlich and Langmuir models. Also the adsorption of oil onto the hydrogel behaves as a pseudo-second-order kinetic models rather than the pseudo-first-order kinetic model...
  80. ncbi Copper corrosion-simulated uterine solutions
    J M Bastidas
    National Center for Metallurgical Research CSIC, Avda Gregorio del Amo 8, 28040 Madrid, Spain
    Contraception 61:395-9. 2000
    ..4-4.5 microg/day for 15 days and 0.1-0.3 microg/day for 360 days. Of the order of 30%-40% of dissolved cupric ions were trapped in the product layers. The main compounds identified were cuprite (Cu(2)O), calcite (CaCO(3)), and phosphates...
  81. ncbi Cryopreservation of human bone marrow-derived mesenchymal stem cells with reduced dimethylsulfoxide and well-defined freezing solutions
    Yang Liu
    Dalian R and D Center for Stem Cell and Tissue Engineering, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, PR China
    Biotechnol Prog 26:1635-43. 2010
    The aim of this study is to investigate the feasibility of using well defined, serum-free freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2...
  82. ncbi Generalized concentration dependence of globular protein self-diffusion coefficients in aqueous solutions
    Irina V Nesmelova
    Laboratory of Molecular Biophysics, Kazan Institute of Biochemistry and Biophysics, Kazan 420503, Russia
    Biopolymers 63:132-40. 2002
    ..self-diffusion coefficients of globular proteins (myoglobin, bovine serum albumin, barstar, lysozyme) in aqueous solutions at different temperatures and pH values are obtained by pulsed-gradient spin-echo NMR, and their concentration ..
  83. pmc APOBEC2 is a monomer in solution: implications for APOBEC3G models
    Troy C Krzysiak
    Department of Structural Biology, University of Pittsburgh School of Medicine, and Pittsburgh Center for HIV Protein Interactions, Pittsburgh, Pennsylvania 15261, United States
    Biochemistry 51:2008-17. 2012
    ..In summary, our results cast doubt on all previous structure-function predictions for APOBEC3G that were based on the crystal structure of APOBEC2...
  84. ncbi The influence of charge distribution on self-association and viscosity behavior of monoclonal antibody solutions
    Sandeep Yadav
    Late Stage Pharmaceutical Development, Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Mol Pharm 9:791-802. 2012
    ..However, replacement of charge residues in the sequence of MAb-2, M-10, did not invoke charge distribution to the same extent as MAb-1 and hence exhibited a similar viscosity and self-association profile as MAb-2...
  85. ncbi Plasma glucagon and insulin responses depend on the rate of appearance of amino acids after ingestion of different protein solutions in humans
    Jose A L Calbet
    Copenhagen Muscle Research Center, Rigshospitalet, Section 7652, Blegdamsvej 9, Denmark
    J Nutr 132:2174-82. 2002
    To find out whether the hormonal response to feeding with protein solutions is influenced by the nature and degree of protein fractionation, we examined insulin and glucagon responses after intake of protein solutions containing the same ..
  86. pmc Minireview: structural insights into early folding events using continuous-flow time-resolved small-angle X-ray scattering
    Sagar V Kathuria
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Biopolymers 95:550-8. 2011
    ..Current improvements and future prospects of microsecond time-resolved SAXS and the synergy with ab initio structure prediction and molecular dynamics simulations are discussed...
  87. ncbi Cluster-driven dynamical arrest in concentrated lysozyme solutions
    Frédéric Cardinaux
    Department of Physics, University of Fribourg, Fribourg, Switzerland
    J Phys Chem B 115:7227-37. 2011
    ..a detailed experimental and numerical study of the structural and dynamical properties of salt-free lysozyme solutions. In particular, by combining small-angle X-ray scattering (SAXS) data with neutron spin echo (NSE) and rheology ..
  88. pmc X-ray solution scattering studies of the structural diversity intrinsic to protein ensembles
    Lee Makowski
    Department of Electrical and Computer Engineering, Northeastern University, Boston, MA 02115, USA
    Biopolymers 95:531-42. 2011
    ..The insights which these analyses support are discussed in terms of the function of the various proteins...
  89. ncbi Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector
    Kengo Suzuki
    Hamamatsu Photonics K K, Joko cho, Hamamatsu 431 3196, Japan
    Phys Chem Chem Phys 11:9850-60. 2009
    We reevaluate the absolute fluorescence and phosphorescence quantum yields of standard solutions by using a novel instrument developed for measuring the absolute emission quantum yields of solutions...
  90. pmc Characterization of the decaheme c-type cytochrome OmcA in solution and on hematite surfaces by small angle x-ray scattering and neutron reflectometry
    A Johs
    Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA
    Biophys J 98:3035-43. 2010
  91. pmc Solution structure of the human signaling protein RACK1
    Kaliandra A Gonçalves
    Laboratório Nacional de Biociências LNBio, Centro de Pesquisa em Energia e Materiais CNPEM, Campinas, SP, Brazil
    BMC Struct Biol 10:15. 2010
    ..RACK1 is ubiquitously expressed and has been implicated in diverse cellular processes involving: protein translation regulation, neuropathological processes, cellular stress, and tissue development...
  92. ncbi Solution NMR studies of the A beta(1-40) and A beta(1-42) peptides establish that the Met35 oxidation state affects the mechanism of amyloid formation
    Liming Hou
    Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106 7078, USA
    J Am Chem Soc 126:1992-2005. 2004
  93. ncbi Storage of platelets in additive solutions: the effects of magnesium and potassium on the release of RANTES, beta-thromboglobulin, platelet factor 4 and interleukin-7, during storage
    A Shanwell
    Department of Transfusion Medicine, Huddinge University Hospital, Stockholm, Sweden
    Vox Sang 85:206-12. 2003
    ..Therefore, we investigated whether the addition of magnesium and potassium to platelets stored in a platelet additive solution (PAS) would affect the generation of cytokines during platelet storage...
  94. ncbi Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy
    Yuki Abe
    Department of Biochemical Engineering, University College London, UK
    Biochem J 432:101-11. 2010
    ..The present results clarify IgG4 function and provide a starting point to investigate antibody stability...
  95. ncbi Metastable liquid clusters in super- and undersaturated protein solutions
    Olga Gliko
    Department of Chemical and Biomolecular Engineering, University of Houston, Houston, TX 77204, USA
    J Phys Chem B 111:3106-14. 2007
    ..metastable with respect to a solid phase, but stable with respect to the solution, have been known to form in solutions of proteins and small-molecule substances...
  96. ncbi Recognition of caffeine in aqueous solutions
    Roberto Fiammengo
    Laboratory of Supramolecular Chemistry and Technology MESA Research Institute, University of Twente, 7500 AE Enschede, The Netherlands
    Chemistry 9:784-92. 2003
    Binding of caffeine in aqueous solutions has been achieved for the first time by using water-soluble, tetracationic peptide-porphyrin conjugates Zn-1 as the receptor molecules...
  97. ncbi Structure determinations of human and chimaeric antibodies by solution scattering and constrained molecular modelling
    Stephen J Perkins
    Department of Biochemistry and Molecular Biology, Darwin Building, University College London, Gower Street, London WC1E 6BT, U K
    Biochem Soc Trans 36:37-42. 2008
    ..Constrained modelling confirms the experimental data analysis and produces families of best-fit molecular models. Its usage has clarified several aspects of antibody structure and function in solution...
  98. pmc Detecting and minimizing zinc contamination in physiological solutions
    Alan R Kay
    Dept of Biological Sciences, 138 BB, University of Iowa, Iowa City, IA 52242, USA
    BMC Physiol 4:4. 2004
    ..physiology it is important to be able to control and quantify the level of Zn contamination in experimental solutions. A technique that relies on a Zn-sensitive fluorimetric probe is introduced for measuring Zn concentrations as ..
  99. ncbi In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite
    Morgana Eli Vianna
    Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, SP, Brazil
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:79-84. 2004
    ..0% and 2.0% CHX liquid to eliminate all microorganisms was the same required for 5.25% NaOCl. The antimicrobial action is related to type, concentration, and presentation form of the irrigants as well as the microbial susceptibility...
  100. ncbi Lead removal from aqueous solution by natural and pretreated clinoptilolite: adsorption equilibrium and kinetics
    Ahmet Gunay
    Chairmenship of Environmental Protection Department, Antalya Great Municipality, Antalya, Turkey
    J Hazard Mater 146:362-71. 2007
    ..The negative value of change in Gibbs free energy (DeltaG degrees ) indicates that adsorption of Pb(II) on clinoptilolite is spontaneous...
  101. ncbi Ultrafast x-ray diffraction of transient molecular structures in solution
    H Ihee
    Department of Chemistry and School of Molecular Science BK21, Korea Advanced Institute of Science and Technology KAIST, Daejeon, 305 701, Republic of Korea
    Science 309:1223-7. 2005
    ..Solvent dynamics were also extracted from the data, revealing a change in the solvent cage geometry, heating, and thermal expansion...

Research Grants80

  1. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ..xerostomia-based treatments are inadequate and temporary thus creating a significant clinical need for long-term solutions that include replacing irreversibly damaged or lost salivary tissue, with functional tissue grown on ..
  2. Tobacco Control Research Branch Scientific and Technical Services Support
    KOURTNEY MARSHALL; Fiscal Year: 2009
    ..submit an annual report of no more than five (5) (Is this too specific or restrictive, or appropriate?) pages that briefly summarizes results and problems encountered (with their respective solutions) during the covered contract period.
    Sridhar Sunderam; Fiscal Year: 2013
    ..EEG is therefore a critical barrier to the discovery of genes that promote sleep disorders. Signal Solutions, LLC, has developed a sensor cage environment for noninvasive, HT behavioral monitoring that is being used by ..
  4. Effectivenss of a New Non-Invasive Erectile Support System in Aging Populations
    S ALEXANDER WEINSTOCK; Fiscal Year: 2012
    ..b>Solutions include: Over-the-counter chemical remedies, mechanical vacuum devices, still controversial prescribed pills, ..
  5. A Computational Framework for Mapping Long Range Genetic Circuits
    Tolga Tasdizen; Fiscal Year: 2010
    ..Creating solutions to understand these brain systems will broadly accelerate unraveling the connectivity of many circuits that are ..
  6. Validation of a Novel NF-kB Inhibitor in Inflammatory Bowel Disease
    Anthony Blikslager; Fiscal Year: 2013
    ..The multidisciplinary team assembled for this phase 2 proposal has developed innovative solutions to these hurdles...
  7. Repression of PPARgamma-2 by Glucocorticoid-Induced Leucine Zipper
    Xing Ming Shi; Fiscal Year: 2012
    ..are major public health threats, understanding how PPAR(2 expression can be negatively regulated will provide solutions not only for treating osteoporosis, but obesity and obesity-related disorders as well...
  8. Nitric Oxide Signaling with Heme Proteins
    William R Montfort; Fiscal Year: 2012
    ..NO is a reactive molecule with a short half-life in biological solutions and many of the reaction products are toxic...
  9. Biophysics of the morphology and motility of Borrelia burgdorferi in diverse envi
    Charles W Wolgemuth; Fiscal Year: 2013
    ..directed toward moving the current understanding of motility in non-physiological liquid and/or methycellulose solutions to biologically realistic environments in which spirochetes adhere to cells or ECM in order to complete their ..
  10. Biomarkers of Fusarium biofilm formation in vitro and in vivo
    PRANAB KUMAR MUKHERJEE; Fiscal Year: 2012
    ..we showed that: (1) Fusarium forms biofilms on soft contact lenses, which were resistant to lens cleaning solutions, (2) Fusarium biofilms formed on silicone hydrogel contact lenses induce keratitis on injured corneas in our ..
  11. Avoiding toxicity associated with MTP ablation
    M Mahmood Hussain; Fiscal Year: 2013
    ..will find out molecular mechanisms responsible for unwanted side effects associated with MTP therapy and suggest solutions to avoid these toxicities...
  12. Nikkomycin Z treatment of early coccidioidal pneumonia: Phase II clinical trial
    DAVID LARWOOD; Fiscal Year: 2010
    ..If commercialization of nikkomycin Z is successful, the Valley Fever Solutions business plan projects $250 million annual revenue within 5 years of NDA approval...
  13. Developmental Regulation of Brain and Behavior
    JULI S WADE; Fiscal Year: 2011
    ..of factors critical to normal development of brain morphology and behavior, and can lead to the discovery of solutions to problems, including those associated with mental health disorders such as autism, schizophrenia and ..
    CLARK ALGAIER; Fiscal Year: 2009
    ..During the Fiscal Year hundreds of chemicals were tested for solubility, prepared 10/20mM solutions and shipped to different labs for high through-put screening experiments...
  15. Translational Approaches to Reducing Health Disparities in Lung Disease
    Joe G N Garcia; Fiscal Year: 2012 various lung diseases, and highlight the existing health disparities with the idea of finding appropriate solutions. The proposed conference will focus on five major thematic areas: 1...
  16. RCT of US versus CT for Patients in the ED with Suspected Renal Colic
    Rebecca Smith Bindman; Fiscal Year: 2010
    ..ED overcrowding is on the verge of crisis according to the Institute of Medicine, and one of the solutions identified is to increase the efficiency of patient care in the ED setting...
  17. Genetic and Cellular Basis of Resistance/Sensitivity to Myocardial Ischemia
    Howard J Jacob; Fiscal Year: 2012
    ..The significance of this aim is proving a mutation is casual and uses innovative solutions to generate the transgenic rescue animals...
  18. Bioconductor: an open computing resource for genomics
    Martin Morgan; Fiscal Year: 2010
    ..Software solutions must be able to rapidly adapt and to facilitate new problems when they arise...
  19. Histone Deacetylation Regulates Growth Plate Development
    Lei Wei; Fiscal Year: 2013
    ..Leg length discrepancy and dwarfism are just a few clinical dilemmas that may be provided with more elegant solutions if the molecular triggers and stops to the growth plate can be elucidated...
    ..Importantly we are following up on new pilot data that suggest that young rats are more motivated for sweet solutions than adult rats in our lab, and we observe an effect of high fat diet...
  21. Financial Risks and Ethnic/Racial Disparities in Live Kidney Donation
    TANJALA PURNELL; Fiscal Year: 2011
    ..Bloomberg School of Public Health, Johns Hopkins School of Medicine, Johns Hopkins Center for Health Disparities Solutions, and the Johns Hopkins Welch Center for Prevention, Epidemiology, and Clinical Research on projects directly ..
  22. Recombinant Polymers for Transarterial Chemoembolization of Liver Cancer
    AZADEH POURSAID; Fiscal Year: 2013
    ..Aqueous solutions of SELP are unique in that they remain soluble until injected into the body where they transition irreversibly ..
  23. Scripps Translational Science Institute (TL1)
    ERIC JEFFREY TOPOL; Fiscal Year: 2013
    ..Table 3) highlighting the culture of collaboration amongst clinical and basic scientists to pursue basic science solutions ,to important medical challenges...
  24. Statistical models to investigate long-distance QTL transcription regulation
    ..use Markov chain Monte Carlo and linear programming relaxation methods that have been shown to find near-optimal solutions to this type of problem...
    JEFFREY SCOTT BARRETT; Fiscal Year: 2010
    ..pharmacology and health IT sectors we envision a heightened appreciation for pharmacology-based decision support solutions and an evolving strategy to use such solutions to improve outcomes in children...
  26. The XXII nd North American Testis Workshop
    WILLIAM WALLACE WRIGHT; Fiscal Year: 2013
    ..on the regulation and function of the fertile and infertile testis The first goal of this meeting is to promote solutions for two major medical and public health problems: the high incidence of male infertility in the developed world ..
  27. Development of a Recombinant Subunit Vaccine for CCHF
    David E Clements; Fiscal Year: 2013
    ..The NIAID Strategic Plan for Biodefense Research is specifically directed at promoting research that can provide solutions to mitigate the threat posed by Category A, B, and C priority pathogens...
  28. Molecular genetic control of the immature female mosquito vector
    Helen Benes; Fiscal Year: 2012 female mosquitoes continue to pose threats to health and life and to challenge researchers to find novel solutions to vector control and pathogen transmission...
  29. Directed differentiation of embryonic stem cells for urologic applications
    James A Bassuk; Fiscal Year: 2010
    ..stem cells, (ii) generating new in vitro models of urothelial cell differentiation and (iii) tissue engineered solutions for urologic use...
  30. Preventing Human Prion Diseases - Inactivation of Prions
    Stanley B Prusiner; Fiscal Year: 2010
    ..have been extended to the study of denaturing detergents, including sodium dodecyl sulfate (SDS) in acidic solutions. We have determined that treatment with acidic SDS combined with autoclaving reduces the infectivity of Syrian ..
  31. Specific Contribution of a New IG Isotype (IGT) in Teleost Fish Immune Responses
    J Oriol Sunyer; Fiscal Year: 2013
    ..Thus, some of the novel immunological solutions driven by these selective forces in fish and mammals are likely to share fundamental structural and mechanistic ..
  32. Workplace Health Promotion
    MARGARET A HANNON; Fiscal Year: 2010
    ..goal of this application is to enhance the dissemination potential of a successful intervention, Workplace Solutions that was developed to disseminate a set of 15 evidence- based cancer prevention strategies to workplaces...
  33. Technology platform for development of multi-component preservation solution
    Allison Hubel; Fiscal Year: 2013
    ..Testing solutions using multiple compounds would normally require thousands of experiments...
  34. Alkylammonium Formate Mobile Phase Modifiers for Protein Liquid Chromatography
    NEIL DANIELSON; Fiscal Year: 2009
    ..should be more stable in mixtures of buffer and MAF or EAF than corresponding buffer-organic solvent solutions permitting their use as analytical reagents...
  35. GHSU/WSU Cooperative Reproductive Medicine Network
    Michael Peter Diamond; Fiscal Year: 2013
    ..on the Network's capacity to attain its goal of identifying and/or improving diagnostic and therapeutic solutions to problems in reproductive medicine...
  36. Sorbents for Toxic-Metal Removal in Pharmaceutical Development and Manufacture
    GIRISH SRINIVAS; Fiscal Year: 2013
    ..The carbons will be sold in canisters;pharmaceutical mixtures purified by eluting solutions through packed beds of the activated carbons.
    Martin Morad; Fiscal Year: 2013
    ..These signals are suppressed by Na+-free or CGP-37157- containing solutions, implicating mNCX in mediating the Ca2+ release process...
  38. Thromboresistant Polymers via Catalytic Generation of NO
    Mark E Meyerhoff; Fiscal Year: 2012 organoselenium (RSe) species are capable of generating physiologically relevant levels of NO when bathed in solutions containing 5M levels of RSNOs, the concentrations of RSNOs found in fresh blood...
    John J Eppig; Fiscal Year: 2012
    ..Resolution of these mechanisms of intercellular signaling will lead to novel clinical solutions to female infertility.
  40. GE Healthcare Discovery MR750 3T MRI System
    FRANK KOROSEC; Fiscal Year: 2010
    ..Acquisition of the MR750 has positive impact on the environment, since GE has several "green" solutions such as a refurbishment and recycling program where 94% of materials are reused, redeployed, or recycled, and ..
  41. A New Low Power Low Cost Ozone Sterilizer for Medical Equipment
    Joseph R Stetter; Fiscal Year: 2012
    ..These sterilization methods, while adaptable for hospitals, cannot be used as portable sterilization solutions for field operations...
  42. Translational Optimization of Bone Regeneration in the Irradiated Mandible
    Steven R Buchman; Fiscal Year: 2013
    ..synergies and potentially be isolated and manipulated directly in the operating room still require innovative solutions in order to be fully optimized for translation to the clinical aena...
  43. The role of taste in amino acid appetite
    Ivan E de Araujo; Fiscal Year: 2013
    ..neurochemical measurements in wild-type and Trpm5 knockout mice exposed to different carbohydrate and amino acid solutions. Importantly, by testing the glucose utilization hypothesis, the proposed project will provide novel data on how ..
  44. Hepatocyte - Based Therapies of Primary Hyperoxaluria 1
    Namita Roy Chowdhury; Fiscal Year: 2010
    ..Three recent developments in our laboratory offer potential solutions to these problems...
  45. Acuson Sequoia C512 Echocardiography System
    Sandrine V Pierre; Fiscal Year: 2010
    ..proposal to acquire an ACUSON SequoiaTM C512 cardiac ultrasound imaging system, manufactured by Siemens Medical Solutions USA, Inc...
  46. Crystalline hydroxocobalamin and methemoglobin as a treatment of H2S intoxication
    PHILIPPE A HAOUZI; Fiscal Year: 2013
    ..The goal of this project is to test the hypothesis that solutions of methemoglobin or Vitamin B12 can be used as antidotes following H2S exposure...
  47. Role of Fgfr2IIIb in epithelial mesenchymal signaling in intestinal development
    Peter F Nichol; Fiscal Year: 2013
    ..collaborative efforts in this area as well as direct attention and resources towards developing therapies and solutions for this problem. All of these steps will facilitate my successful transition to independence...
    Diane J Mathis; Fiscal Year: 2013
    ..The resulting cross-fertilization will almost certainly continue to result in novel solutions to the persisting problems posed by autoimmune disease.
  49. RRSS #1 - Evaluation of Process Efficiency in the Task of Case Consolidation with
    Kevin Ward; Fiscal Year: 2009
    ..Best practices should be reviewed and possible solutions brainstormed. Steps that hinder productivity through waste should be eliminated...
  50. Optimization of Protein Therapeutics Formulations
    Lawrence J Delucas; Fiscal Year: 2012
    ..and physical stability of each protein by measuring the second virial coefficient (B-value) for the protein in solutions containing different excipients...
  51. Determinants of growth and fitness in drug resistant malaria parasites
    Michael T Ferdig; Fiscal Year: 2012
    ..drug resistance adaptations, we focus on basic growth and normal cellular processes in two different parasite solutions to historical drug pressure: 1) progeny of a genetic cross that maintain a common pfcrt allele in a segregating ..
  52. Aging Stratum Corneum pH and Barrier Function
    Theodora M Mauro; Fiscal Year: 2012
    ..Further, we have found that applying acidic solutions to moderately aged skin restores skin acidity and normal lipid processing, thus improving the epidermal barrier...
    Sridhar Madala; Fiscal Year: 2010
    ..However, existing solutions for continuous noninvasive monitoring require cumbersome cuffs and are unsuitable for true ambulatory use...
  54. Sound Source Segregation
    Mark A Bee; Fiscal Year: 2013
    ..proposed here employs similar logic based on the premise that evolution is well known for finding diverse solutions to common problems in different animal lineages...
  55. Structure and Function of S-adenosyl-L-methionine Riboswitches
    Ailong Ke; Fiscal Year: 2013
    ..These RNAs represent three independent evolution solutions to achieve specific SAM recognition...
  56. Center for Protein Folding Machinery
    Vijay S Pande; Fiscal Year: 2013
    ..our highly interdisciplinary strategy and collaboration to develop cutting-edge methodologies and therapeufic solutions. Our approach consists of 4 major aims aligned with stages in the therapeutics discovery process: [unreadable] ..
  57. Structural and Dynamical Response of Escherichia coli to Osmotic Stress
    James C Weisshaar; Fiscal Year: 2010
    ..Remarkably little is known about how to extend kinetics and thermodynamic results from dilute solutions to the crowded, complex environment of the cytoplasm...
    Ying Xia; Fiscal Year: 2013
    ..underlying the EA-induced protection from cerebral ischemia and may provide novel clues for therapeutic solutions of stroke...
  59. Ubiquitous Mobile Multimedia for Environmental Public Health Outreach and Social
    CESAR BANDERA; Fiscal Year: 2010
    ..of any user to post multimedia content on a social network web site from any cell phone, require technical solutions to the current lack of interoperability...
  60. Prospective Validation and Cost Analysis of the National Guidelines for Field Tri
    Craig D Newgard; Fiscal Year: 2011
    ..Importance: Data-driven solutions to increase trauma system efficiency, reduce costs, and preserve valuable resources, while still maximizing ..
  61. Statistical models to investigate long-distance QTL transcription regulation
    ..use Markov chain Monte Carlo and linear programming relaxation methods that have been shown to nd near-optimal solutions to this type of problem...
  62. Lysosomal Disease Network
    Chester B Whitley; Fiscal Year: 2013
    ..b>Solutions to these problems will have direct impact on patients suffering from lysosomal diseases, and important ..
  63. Passivating Proteins in Implantable Glucose Sensors
    W Ward; Fiscal Year: 2004
    ..The degree to which four proteins in buffered solutions will adsorb to polyurethane-coated surfaces will be measured using Iodine-125 radiolabeled proteins...
    PETER STEPONKUS; Fiscal Year: 1993
    ..and mixtures of cryoprotectants in the loading step and (2) the development of improved vitrification solutions and procedures for their use that reduce their toxicity...
  65. Exchangeable Heart Valve for Tissue Engineering Applications
    Ivan Vesely; Fiscal Year: 2007
    ..VXI will thus continue to advance the field of prosthetic valve technologies into new areas where innovative solutions for patients with heart valve disease are needed...
  66. Betahistine: Novel Therapeutic in Attention Deficit Hyperactivity Disorder
    CHARLES PIERCE; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Satiety Solutions, is developing a novel histamine agonist, betahistine, as a treatment for attention-deficit, hyperactivity disorder (ADHD)...
  67. New Solutions to Improve Islet Recovery after Machine Preservation of Pancreas
    Michael Taylor; Fiscal Year: 2009
    ..The primary objective of this proposal is to use state-of-the-art perfusion technology and new preservation solutions to test the hypothesis that machine perfusion can improve the yield and viability of islets prepared from ..
  68. Artificial Cornea Using New Biomaterials
    MAX MAGINNESS; Fiscal Year: 2009
    ..It affects some 12 million people worldwide. [1] Corneal transplants with human donor tissue offer solutions for a fraction of patients with less severe disease, with approximately 100,000 such procedures worldwide every ..
  69. An injectable biocompatible hydrogel nucleus pulposus replacement
    Gavin Braithwaite; Fiscal Year: 2009
    ..Although commonly prescribed, moderately invasive surgical solutions such as discectomy and laminectomy, and more invasive solutions such as intervertebral fusion and total disc ..
  70. Iron Delivery Via hemodialysate in ESRD
    Ajay Gupta; Fiscal Year: 2005
    ..FePPi), a monomeric iron salt (745 Da), can be delivered directly into the circulation when added to dialysis solutions. Fe(III) complexes tightly with pyrophosphate (PPi), thereby reducing dissociation and release of free iron...
  71. Integrating Patient Generated Family Health History from Varied EHR Entry Portals
    Jennifer S Haas; Fiscal Year: 2010
    ..b>Solutions do not currently exist by which most Americans can organize their family health history and then place it into ..
  72. DNA and RNA Stability in Glycine Betaine, TMAO, and Urea Solutions: Correlating S
    JEFFREY JAMES contact SCHWINEFUS; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Cellular biochemical reactions involving nucleic acids occur in aqueous solutions of salts, cosolutes, and biopolymers, such as proteins...
  73. Ultraprecision Diamond Machining of Conventional Non-Diamond Machinable Materials
    Gang Zhang; Fiscal Year: 2007
    ..Current technical solutions for extending diamond tool life only achieve limited success; suffer from high cost and complicated setup; and ..
  74. Enhancing the Obesity Prevention Program at Harvard's DACP
    Matthew W Gillman; Fiscal Year: 2010
    ..Yet the origins of obesity remain poorly understood and many potential solutions to the problem are understudied...
    Pamela Whitten; Fiscal Year: 2000 research objective 23: Care for Older People For the past two decades, policy analysts have struggled to find solutions for rising long-term care costs for chronically impaired older persons...
    Sherrill Slichter; Fiscal Year: 2009
    ..evaluating the effects of an initial collection injury that may limit storage duration and the role of storage solutions in facilitating extended platelet storage. Dr...
  77. Modular internalizing molecules for treatment of more than one stage of malignanc
    Michael Lawrence Greenberg; Fiscal Year: 2010
    ..have tremendous potential in therapeutic applications but their development as therapeutics requires suitable solutions for a) cell-specific targeting;b) delivery to the RNAi processing machinery as well as c) systemic delivery of ..
    Jatinder Bhatia; Fiscal Year: 1990
    ..The detrimental photooxidation processes could be initiated when the TPN solutions are exposed to light, particularly the intense ambient light in neonatal nurseries, or when the solutions are in ..
  79. Effects of Hyaluronan on Interstitial Convection
    MICHAEL FLESSNER; Fiscal Year: 2007
    ..factor determining interstitial transport properties of the barrier between the blood and therapeutic dialysis solutions in the peritoneal cavity...
  80. The Seven Pillars: Crossing the Patient Safety-Medical Liability Chasm
    TIMOTHY BRIAN MCDONALD; Fiscal Year: 2010
    ..S. health care system. Innovative strategies combining solutions to minimize preventable harms while curbing medical liability risk are rare...