selenomethionine

Summary

Summary: Diagnostic aid in pancreas function determination.

Top Publications

  1. ncbi Differential responses to selenomethionine supplementation by sex and genotype in healthy adults
    Gerald F Combs
    Grand Forks Human Nutrition Research Center, USDA ARS, 2420 2nd Avenue North, Stop 9034, Grand Forks, ND 58202 9034, USA
    Br J Nutr 107:1514-25. 2012
  2. pmc Superior in vivo inhibitory efficacy of methylseleninic acid against human prostate cancer over selenomethionine or selenite
    Guang Xun Li
    Hormel Institute, University of Minnesota, 801 16th Avenue Northeast, Austin, MN 55912, USA
    Carcinogenesis 29:1005-12. 2008
  3. ncbi Effects of a six month treatment with selenomethionine in patients with autoimmune thyroiditis
    Leonidas H Duntas
    Endocrine Unit, Evgenidion Hospital, University of Athens Medical School, 20 Papadiamantopoulou Str, 11528 Athens, Greece
    Eur J Endocrinol 148:389-93. 2003
  4. ncbi Effects of 12 months treatment with L-selenomethionine on serum anti-TPO Levels in Patients with Hashimoto's thyroiditis
    Elias E Mazokopakis
    Department of Internal Medicine, University Hospital of Heraklion, Crete, Greece
    Thyroid 17:609-12. 2007
  5. ncbi Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice
    Lin Yan
    U S Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202, USA
    Int J Cancer 131:1260-6. 2012
  6. ncbi Dietary selenomethionine increases exon-specific DNA methylation of the p53 gene in rat liver and colon mucosa
    Huawei Zeng
    USDA, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, USA
    J Nutr 141:1464-8. 2011
  7. pmc Selenomethionine regulation of p53 by a ref1-dependent redox mechanism
    Young R Seo
    Department of Microbiology, Walther Oncology Center, and Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, IN 46208, USA
    Proc Natl Acad Sci U S A 99:14548-53. 2002
  8. ncbi Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
    Hassan K Sreenath
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
    Protein Expr Purif 40:256-67. 2005
  9. ncbi Dietary supplementation of selenomethionine reduces metastasis of melanoma cells in mice
    L Yan
    Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA
    Anticancer Res 19:1337-42. 1999
  10. ncbi Selenite and selenomethionine promote HL-60 cell cycle progression
    Huawei Zeng
    U S Department of Agriculture, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202 9034, USA
    J Nutr 132:674-9. 2002

Detail Information

Publications273 found, 100 shown here

  1. ncbi Differential responses to selenomethionine supplementation by sex and genotype in healthy adults
    Gerald F Combs
    Grand Forks Human Nutrition Research Center, USDA ARS, 2420 2nd Avenue North, Stop 9034, Grand Forks, ND 58202 9034, USA
    Br J Nutr 107:1514-25. 2012
    ..to characterise the responses of multiple biomarkers of Se status in healthy American adults to supplemental selenomethionine (SeMet) and to identify factors affecting those responses...
  2. pmc Superior in vivo inhibitory efficacy of methylseleninic acid against human prostate cancer over selenomethionine or selenite
    Guang Xun Li
    Hormel Institute, University of Minnesota, 801 16th Avenue Northeast, Austin, MN 55912, USA
    Carcinogenesis 29:1005-12. 2008
    ..methylselenol precursors methylseleninic acid (MSeA) and Se-methylselenocysteine (MSeC) in comparison with selenomethionine (SeMet) and selenite in DU145 and PC-3 human PCa xenografts in athymic nude mice...
  3. ncbi Effects of a six month treatment with selenomethionine in patients with autoimmune thyroiditis
    Leonidas H Duntas
    Endocrine Unit, Evgenidion Hospital, University of Athens Medical School, 20 Papadiamantopoulou Str, 11528 Athens, Greece
    Eur J Endocrinol 148:389-93. 2003
    ..Therefore, we performed a randomised, placebo-controlled prospective study to investigate the effects of Se treatment on patients with autoimmune thyroiditis (AIT)...
  4. ncbi Effects of 12 months treatment with L-selenomethionine on serum anti-TPO Levels in Patients with Hashimoto's thyroiditis
    Elias E Mazokopakis
    Department of Internal Medicine, University Hospital of Heraklion, Crete, Greece
    Thyroid 17:609-12. 2007
    ..We studied the effects of selenium (Se) treatment on serum anti-thyroid peroxidase (TPO) levels in Greek patients with Hashimoto's thyroiditis (HT)...
  5. ncbi Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice
    Lin Yan
    U S Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202, USA
    Int J Cancer 131:1260-6. 2012
    ..investigated the effects of dietary supplementation with methylseleninic acid (MSeA), in comparison with selenomethionine (SeMet), on spontaneous metastasis of Lewis lung carcinoma (LLC) in male C57BL/6 mice using intramuscular and ..
  6. ncbi Dietary selenomethionine increases exon-specific DNA methylation of the p53 gene in rat liver and colon mucosa
    Huawei Zeng
    USDA, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, USA
    J Nutr 141:1464-8. 2011
    ..15 [Se adequate (A)], or 4 mg [Se supranutritional (S)] (Se as l-selenomethionine)/kg diet for 104 d, respectively...
  7. pmc Selenomethionine regulation of p53 by a ref1-dependent redox mechanism
    Young R Seo
    Department of Microbiology, Walther Oncology Center, and Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, IN 46208, USA
    Proc Natl Acad Sci U S A 99:14548-53. 2002
    ..We show that selenium in the form of selenomethionine (SeMet) can activate the p53 tumor suppressor protein by a redox mechanism that requires the redox factor ..
  8. ncbi Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
    Hassan K Sreenath
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
    Protein Expr Purif 40:256-67. 2005
    Protocols have been developed and applied in the high-throughput production of selenomethionine labeled fusion proteins using the conditional Met auxotroph Escherichia coli B834...
  9. ncbi Dietary supplementation of selenomethionine reduces metastasis of melanoma cells in mice
    L Yan
    Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA
    Anticancer Res 19:1337-42. 1999
    The present study investigated the effect of dietary supplementation of selenomethionine on pulmonary metastasis of B16BL6 murine melanoma cells in C57BL/6 mice. Mice were assigned to four groups of 15 each...
  10. ncbi Selenite and selenomethionine promote HL-60 cell cycle progression
    Huawei Zeng
    U S Department of Agriculture, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202 9034, USA
    J Nutr 132:674-9. 2002
    ..In this study, the effects of selenium deficiency or adequacy (0.25 micromol/L selenite or selenomethionine) on HL-60 cell cycle progression were examined in serum-free media...
  11. ncbi Methioninase cancer gene therapy with selenomethionine as suicide prodrug substrate
    K Miki
    AntiCancer Incorporated, San Diego, California 92111, USA
    Cancer Res 61:6805-10. 2001
    ..This gene therapy strategy exploits the toxic pro-oxidant property of methylselenol, which is released from selenomethionine (SeMET) by cancer cells with the adenoviral-delivered methionine alpha,gamma-lyase (MET) gene cloned from ..
  12. ncbi Methylselenol generated from selenomethionine by methioninase downregulates integrin expression and induces caspase-mediated apoptosis of B16F10 melanoma cells
    Aeyung Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
    J Cell Physiol 212:386-400. 2007
    ..In this study, we examine the effects of methylselenol generated from selenomethionine (SeMet) by methioninase (METase) on cell proliferation, adhesion, and expression of integrins in murine ..
  13. ncbi Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice
    Huali Wang
    University of Science and Technology of China, Hefei 230052, People s Republic of China
    Free Radic Biol Med 42:1524-33. 2007
    ..b>Selenomethionine is considered to be the most appropriate supplemental form due to its excellent bioavailability and lower ..
  14. pmc Optimization of selenoprotein P and other plasma selenium biomarkers for the assessment of the selenium nutritional requirement: a placebo-controlled, double-blind study of selenomethionine supplementation in selenium-deficient Chinese subjects
    Yiming Xia
    Sichuan Provincial Center for Disease Control and Prevention, Chengdu, China
    Am J Clin Nutr 92:525-31. 2010
    ..The intake of selenium needed for optimal health has not been established. Selenoproteins perform the functions of selenium, and the selenium intake needed for their full expression is not known...
  15. ncbi Speciation and bioavailability of selenium in yeast-based intervention agents used in cancer chemoprevention studies
    Erik H Larsen
    Danish Institute for Food and Veterinary Research, Department of Food Chemistry, 19 Mørkhøj Bygade, DK 2860 Søborg, Denmark
    J AOAC Int 87:225-32. 2004
    ..The results for the NPC tablets showed that selenomethionine, together with 3 unidentified selenium compounds, were predominant in the sample hydrolysates...
  16. ncbi Protection against radiation-induced oxidative stress in cultured human epithelial cells by treatment with antioxidant agents
    X Steven Wan
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Int J Radiat Oncol Biol Phys 64:1475-81. 2006
    ..To evaluate the protective effects of antioxidant agents against space radiation-induced oxidative stress in cultured human epithelial cells...
  17. pmc Analysis of methionine/selenomethionine oxidation and methionine sulfoxide reductase function using methionine-rich proteins and antibodies against their oxidized forms
    Dung Tien Le
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 47:6685-94. 2008
    ..Furthermore, we prepared a selenomethionine form of an MRP and found that selenomethionine selenoxide residues can be efficiently reduced ..
  18. ncbi Selenomethionine protects against adverse biological effects induced by space radiation
    Ann R Kennedy
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, 195 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA, USA
    Free Radic Biol Med 36:259-66. 2004
    ..was to characterize HZE particle radiation-induced adverse biological effects and evaluate the effect of D-selenomethionine (SeM) on the HZE particle radiation-induced adverse biological effects...
  19. ncbi Comparative effects of 2 antioxidants, selenomethionine and epigallocatechin-gallate, on catabolic and anabolic gene expression of articular chondrocytes
    Rina Andriamanalijaona
    Laboratory of Connective Tissue Biochemistry, Faculty of Medicine, 14032 Caen Cedex, France
    J Rheumatol 32:1958-67. 2005
    To determine the effects of selenomethionine (Se-met) and epigallocatechin-gallate (EGCg) on gene expression, activation of mitogen-activating kinases, and DNA binding of nuclear factor-kappaB (NF-kappaB) and apolipoprotein-1 (AP-1) in ..
  20. pmc A novel chemopreventive mechanism of selenomethionine: enhancement of APE1 enzyme activity via a Gadd45a, PCNA and APE1 protein complex that regulates p53-mediated base excision repair
    Hwa Jin Jung
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Oncol Rep 30:1581-6. 2013
    ..Our previous study showed that selenomethionine (SeMet) induces p53 activation without genotoxic effects including apoptosis and cell cycle arrest...
  21. ncbi Anticlastogenic and antigenotoxic effects of selenomethionine on doxorubicin-induced damage in vitro in human lymphocytes
    Raquel Alves Dos Santos
    Department of Genetics, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av Bandeirantes, 3900, 14040 900 Ribeirão Preto, SP, Brazil
    Food Chem Toxicol 46:671-7. 2008
    ..The present study investigated the possible protective effects of selenomethionine (SM) on the cytotoxicity, genotoxicity and clastogenicity of the chemotherapic doxorubicin (DXR), a key ..
  22. ncbi The nutritional significance, metabolism and toxicology of selenomethionine
    Gerhard Norbert Schrauzer
    Biological Trace Element Research Institute, 2400 Boswell Rd, Suite 200, Chula Vista, CA 91914, USA
    Adv Food Nutr Res 47:73-112. 2003
    ..The expectation is that lower additions to feedstock of equivalent levels of SeMet will suffice to achieve adequacy than currently approved maximum levels of Se in the form of inorganic Se salts...
  23. ncbi Selenomethionine and selenocysteine double labeling strategy for crystallographic phasing
    Marie Paule Strub
    Centre de Biochimie Structurale, UMR CNRS 5048, UMR 554 INSERM, Universite Montpellier I, 34090 Cedex, Montpellier, France
    Structure 11:1359-67. 2003
    A protocol for the quantitative incorporation of both selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli is described...
  24. ncbi Highly UV-absorbing complex in selenomethionine-substituted alcohol dehydrogenase from Sulfolobus solfataricus
    Antonietta Giordano
    Institute of Protein Biochemistry, C N R, Via G Marconi 10, I 80125 Naples, Italy
    J Proteome Res 3:613-20. 2004
    ..This effect was explained by the formation of Se complex with some groups of protein. It was revealed that this complex does not participate in fluorescence and does not transfer excitation energy to tryptophan or tyrosine residues...
  25. ncbi Selenium accumulation in prostate tissue during a randomized, controlled short-term trial of l-selenomethionine: a Southwest Oncology Group Study
    Anita L Sabichi
    University of Texas M D Anderson Cancer Center, Baylor College of Medicine, Houston, Texas, USA
    Clin Cancer Res 12:2178-84. 2006
    ..Epidemiologic and clinical data suggest that selenium could prevent prostate cancer, but it has not been shown that supplemental selenium leads to an increased concentration of selenium in prostate tissue compared with adjacent tissue...
  26. ncbi Selenomethionine induces p53 mediated cell cycle arrest and apoptosis in human colon cancer cells
    Ajay Goel
    Division of Gastroenterology, Department of Internal Medicine, Baylor University Medical Center and Baylor Research Institute, Dallas, Texas 75246, USA
    Cancer Biol Ther 5:529-35. 2006
    ..However, the molecular mechanisms of action of selenomethionine, an organoselenium compound present in selenized yeast and currently being investigated in human clinical ..
  27. ncbi Protective effects of L-selenomethionine on space radiation induced changes in gene expression
    J Stewart
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, 195 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104 6072, USA
    Radiat Environ Biophys 46:161-5. 2007
    ..studies are to characterize HZE particle radiation induced biological effects and evaluate the effects of L-selenomethionine (SeM) on these adverse biological effects...
  28. ncbi A comparison of the teratogenicity of methylmercury and selenomethionine injected into bird eggs
    Gary H Heinz
    Patuxent Wildlife Research Center, United States Geological Survey, BARC East, Building 308, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Arch Environ Contam Toxicol 62:519-28. 2012
    ..For all three species, selenomethionine alone caused more deformities than did methylmercury alone...
  29. ncbi Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems
    Zhaopeng Li
    Helmholtz Centre for Infection Research SB, Braunschweig, Germany
    Appl Microbiol Biotechnol 92:823-33. 2011
    ..A simple protocol for producing heavy isotope as well as selenomethionine (Se-Met)-labeled proteins was developed using T7-based Escherichia coli expression systems...
  30. pmc Selenomethionine inhibits IL-1β inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) expression in primary human chondrocytes
    A W M Cheng
    Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 19:118-25. 2011
    ....
  31. ncbi Selenium from selenium-rich Spirulina is less bioavailable than selenium from sodium selenite and selenomethionine in selenium-deficient rats
    J Cases
    , , , 34095 Montpellier, France
    J Nutr 131:2343-50. 2001
    ..Se-deficient rats were then repleted with Se (75 microg/kg) by the addition of sodium selenite, selenomethionine (SeMet) or SeSp to the TY basal diet...
  32. ncbi Simultaneous analysis of mercury and selenium species including chiral forms of selenomethionine in human urine and serum by HPLC column-switching coupled to ICP-MS
    F Moreno
    Departamento de Quimica y Ciencia de los Materiales, Facultad de Ciencias Experimentales, Universidad de Huelva, Campus de El Carmen, 21007 Huelva, Spain
    Analyst 135:2700-5. 2010
    ..and analyzing different selenium and mercury species (Se-methylselenocysteine, selenite, selenate, L-selenomethionine, D-selenomethionine, methylmercury and inorganic mercury)...
  33. ncbi Induction of oxidative stress by selenomethionine in isolated hepatocytes of rainbow trout (Oncorhynchus mykiss)
    Sougat Misra
    Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, Canada S7N 5E2
    Toxicol In Vitro 26:621-9. 2012
    Fish are exposed to environmental selenium predominantly in the form of dietary selenomethionine (SeMet)...
  34. pmc 1,4-phenylenebis(methylene)selenocyanate, but not selenomethionine, inhibits androgen receptor and Akt signaling in human prostate cancer cells
    Nicole D Facompre
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Penn State Hershey Cancer Institute, Hershey, 17033, USA
    Cancer Prev Res (Phila) 3:975-84. 2010
    ..Our initial investigations show that the naturally occurring organoselenium compound selenomethionine (SM) and the synthetic 1,4-phenylenebis(methylene)selenocyanate (p-XSC) can inhibit human prostate cancer ..
  35. ncbi Dietary selenomethionine exposure in adult zebrafish alters swimming performance, energetics and the physiological stress response
    J K Thomas
    Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada
    Aquat Toxicol 102:79-86. 2011
    b>Selenomethionine (Se-Met) is the major form of organoselenium present in food...
  36. ncbi The effect of levothyroxine and selenomethionine on lymphocyte and monocyte cytokine release in women with Hashimoto's thyroiditis
    Robert Krysiak
    Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medykow 18, PL 40 752 Katowice, Poland
    J Clin Endocrinol Metab 96:2206-15. 2011
    No previous study determined monocyte- and lymphocyte-suppressing effects of levothyroxine and selenomethionine and assessed whether their coadministration is superior to treatment with only one of these drugs.
  37. pmc Se-adenosyl-L-selenomethionine cofactor analogue as a reporter of protein methylation
    Ian R Bothwell
    Molecular Pharmacology and Chemistry Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    J Am Chem Soc 134:14905-12. 2012
    ..Here we report a SAM surrogate, ProSeAM (propargylic Se-adenosyl-l-selenomethionine), as a reporter of methyltransferases...
  38. ncbi A novel method for detection of selenomethionine incorporation in protein crystals via Raman microscopy
    Alessandro Vergara
    Dipartimento di Chimica, Universita degli Studi di Napoli Federico II, Complesso Monte S Angelo, Via Cinthia, I 80126 Napoli, Italy
    Acta Crystallogr D Biol Crystallogr 64:167-71. 2008
    ..Mass spectrometry is currently used to evaluate the selenomethionine (SeMet) content in solution, but excluding fluorescence spectroscopy at the absorption edge, no other routine ..
  39. ncbi Sites and extent of selenomethionine incorporation into recombinant Cas6 protein by top-down and bottom-up proteomics with 14.5 T Fourier transform ion cyclotron resonance mass spectrometry
    Xu Wang
    Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, Florida 32306, USA
    Rapid Commun Mass Spectrom 24:2386-92. 2010
    b>Selenomethionine-modified proteins can improve X-ray crystallographic structural resolution by multi-wavelength anomalous diffraction (MAD) phasing...
  40. ncbi Contribution of enzymic alpha, gamma-elimination reaction in detoxification pathway of selenomethionine in mouse liver
    T Okuno
    Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Toxicol Appl Pharmacol 176:18-23. 2001
    The objective of this study was to clarify the detoxification pathways of selenomethionine (SeMet) in mouse liver. It has been postulated that SeMet may be metabolized to selenocysteine (SeCyH) via a pathway similar to methionine (Met)...
  41. ncbi Rapamycin suppresses ROS-dependent apoptosis caused by selenomethionine in A549 lung carcinoma cells
    Maiko Suzuki
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Cancer Chemother Pharmacol 67:1129-36. 2011
    ..In this study, we investigated the effects of rapamycin on apoptosis induced by seleno-L-methionine (SeMet) or selenite in A549 cells...
  42. pmc A human model of selenium that integrates metabolism from selenite and selenomethionine
    Meryl E Wastney
    Metabolic Modeling Services Ltd, 7201 Blenheim, New Zealand
    J Nutr 141:708-17. 2011
    ..of this study was to simultaneously compare kinetics of absorbed Se from inorganic selenite (Sel) and organic selenomethionine (SeMet) in healthy participants (n = 31)...
  43. ncbi Non-optimal levels of dietary selenomethionine alter splenocyte response and modify oxidative stress markers in female mice
    Libia Vega
    Sección Externa de Toxicología, Centro de Investigacion y de Estudios Avanzados, Av IPN 2508, San Pedro Zacatenco, Mexico, DF 07360, Mexico
    Food Chem Toxicol 45:1147-53. 2007
    Many studies evaluating the effects of selenium (Se) status on immunity utilize inorganic Se, although selenomethionine (Se-Met) has been suggested to be more bioavailable and less toxic...
  44. pmc Selenomethionine reduces visual deficits due to developmental methylmercury exposures
    Daniel N Weber
    Marine and Freshwater Biomedical Sciences Center, University of Wisconsin Milwaukee, 600 E Greenfield Ave, Milwaukee, WI 53204, United States
    Physiol Behav 93:250-60. 2008
    ..0-0.30 microM MeHg and/or selenomethionine (SeMet) until 24 hpf then placed in clean medium...
  45. ncbi Uptake, distribution, and speciation of selenoamino acids by human cancer cells: X-ray absorption and fluorescence methods
    Claire M Weekley
    School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia
    Biochemistry 50:1641-50. 2011
    ..The uptake, speciation, and distribution of the common selenoamino acid supplements, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys), in A549 human lung cancer cells were investigated using X-ray ..
  46. ncbi Metabolism of 76Se-methylselenocysteine compared with that of 77Se-selenomethionine and 82Se-selenite
    Kazuo T Suzuki
    Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260 8675, Japan
    Toxicol Appl Pharmacol 217:185-95. 2006
    Se-Methylated selenoamino acids, Se-methylselenocysteine (MeSeCys) and selenomethionine (SeMet), are chemically inert storage forms of selenium in selenium-accumulators, and a nutritional and supplemental source...
  47. ncbi Selective labeling of selenomethionine residues in proteins with a fluorescent derivative of benzyl bromide
    Shenhui Lang
    Department of Chemistry, University of Waterloo, Waterloo, Ont, Canada N2L 3G1
    Anal Biochem 359:253-8. 2006
    ..of the bromobenzyl moiety as a reactive group for the targeted fluorescent labeling of methionine and selenomethionine residues in proteins...
  48. pmc Interactions of Cu(I) with selenium-containing amino acids determined by NMR, XAS, and DFT studies
    Hsiao C Wang
    Department of Chemistry, Clemson University, Clemson, South Carolina 29634 0973, USA
    Inorg Chem 50:10893-900. 2011
    ..selenium antioxidant activity, Cu(I) coordination of the selenoamino acids methyl-Se-cysteine (MeSeCys) and selenomethionine (SeMet) was investigated...
  49. ncbi Selenomethionine induction of DNA repair response in human fibroblasts
    Young R Seo
    Indiana University Cancer Center, Department of Microbiology, Walther Oncology Center, Indiana University School of Medicine and Walther Cancer Institute, Indianapolis, IN 46202, USA
    Oncogene 21:3663-9. 2002
    ..We show that selenium in the form of selenomethionine induces a DNA repair response in normal human fibroblasts in vitro, and protects cells from DNA damage...
  50. ncbi Dimethyldiselenide and methylseleninic acid generate superoxide in an in vitro chemiluminescence assay in the presence of glutathione: implications for the anticarcinogenic activity of L-selenomethionine and L-Se-methylselenocysteine
    J E Spallholz
    Department of Ophthalmology, Texas Tech University Health Sciences Center, Selenium Technologies, Inc, Lubbock, TX, USA
    Nutr Cancer 40:34-41. 2001
    The reduction of cancer incidence by dietary supplementation with L-selenomethionine, L-Se-methylselenocysteine, and other methylated selenium compounds and metabolites is believed to be due to the metabolic generation of the ..
  51. pmc Prostatic response to supranutritional selenium supplementation: comparison of the target tissue potency of selenomethionine vs. selenium-yeast on markers of prostatic homeostasis
    David J Waters
    Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907, USA
    Nutrients 4:1650-63. 2012
    ..the Selenium and Vitamin E Cancer Prevention Trial (SELECT) showed no such cancer-protective advantage using selenomethionine (SeMet)...
  52. ncbi Metabolism of selenomethionine by rainbow trout (Oncorhynchus mykiss) embryos can generate oxidative stress
    Vince P Palace
    Department of Fisheries and Oceans, Habitat Impacts Research, Freshwater Institute, 501 University Crescent, Winnipeg, MB, Canada R3T 2N6
    Ecotoxicol Environ Saf 58:17-21. 2004
    ..L-Selenomethionine, the predominant form of selenium in the eggs of oviparous vertebrates, does not generate oxidative radicals ..
  53. ncbi Selenomethionine treatment in patients with autoimmune thyroiditis: a prospective, quasi-randomised trial
    A D Anastasilakis
    Department of Endocrinology, 424 Military Hospital, Thessaloniki, Greece
    Int J Clin Pract 66:378-83. 2012
    ..To test whether selenium administration affects autoantibodies to thyroid peroxidase (anti-TPO) and thyroglobulin (anti-TG) titres in chronic autoimmune (Hashimoto's - HT) thyroiditis...
  54. ncbi Development of stable isotope and selenomethionine labeling methods for proteins expressed in Pseudomonas fluorescens
    Krishna Madduri
    Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, IN 46268, USA
    Protein Expr Purif 65:57-65. 2009
    ..in structure elucidation studies, the availability of target proteins labeled with stable isotopes or selenomethionine is essential for the determination of protein structures...
  55. ncbi The anticancer effects of sodium selenite and selenomethionine on human colorectal carcinoma cell lines in nude mice
    Yang Yang
    National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, People s Republic of China
    Oncol Res 18:1-8. 2009
    ..to evaluate the chemotherapeutic potential of selenium containing compounds such as sodium selenite (SSe) and selenomethionine (SeMet). Three doses of anticancer drugs were used, including 0...
  56. pmc Selenomethionine incorporation into amyloid sequences regulates fibrillogenesis and toxicity
    Javier Martinez
    Instituto de Quimica Fisica Rocasolano, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    PLoS ONE 6:e27999. 2011
    ..Some of the sequences undergoing fibrillation contain critical methionine (Met) residues which in vivo can be synthetically substituted by selenomethionine (SeM) and alter their properties.
  57. ncbi Determination of methionine and selenomethionine in selenium-enriched yeast by species-specific isotope dilution with liquid chromatography-mass spectrometry and inductively coupled plasma mass spectrometry detection
    Shona McSheehy
    Institute for National Measurement Standards, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R9
    Anal Chem 77:344-9. 2005
    b>Selenomethionine (SeMet) and methionine (Met), liberated by acid hydrolysis of selenium-enriched yeast, were quantified by liquid chromatography-mass spectrometry (LC/MS) using standard additions calibrations as well as isotope dilution (..
  58. ncbi Selenomethionine incorporation into a protein by cell-free synthesis
    Takanori Kigawa
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama, 230 0045 Japan
    J Struct Funct Genomics 2:29-35. 2002
    ..b>Selenomethionine substituted proteins are commonly used for this purpose...
  59. ncbi Crystal structure of the rat liver fructose-2,6-bisphosphatase based on selenomethionine multiwavelength anomalous dispersion phases
    Y H Lee
    Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA
    Biochemistry 35:6010-9. 1996
    ..5 A resolution. The selenomethionine-substituted protein was induced in the methionine auxotroph, Escherichia coli DL41DE3, purified, and ..
  60. ncbi Enhancement of methyl methanesulfonate-induced base excision repair in the presence of selenomethionine on p53-dependent pathway
    Hwa Jin Jung
    Department of Pharmacology, Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
    J Med Food 12:340-4. 2009
    b>Selenomethionine (SeMet) has been identified as a chemopreventive antioxidant to activate p53-mediated nucleotide excision repair. In this study, we examined whether p53-mediated base excision repair (BER) might be induced by SeMet...
  61. pmc Selenomethionine and alpha-tocopherol do not inhibit prostate carcinogenesis in the testosterone plus estradiol-treated NBL rat model
    Nur Ozten
    Department of Pathology, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA
    Cancer Prev Res (Phila) 3:371-80. 2010
    ..In this study, we examined the potential of selenomethionine and alpha-tocopherol to modulate prostate cancer development in the testosterone plus estradiol-treated NBL ..
  62. ncbi Selenium and the thyroid: a close-knit connection
    Leonidas H Duntas
    Endocrine Unit, Evgenidion Hospital, University of Athens, 20 Papadiamantopoulou Street 115 28 Athens, Greece
    J Clin Endocrinol Metab 95:5180-8. 2010
    ..It has additionally been established that the thyroid contains more selenium than any other tissue and that selenium deficiency aggravates the manifestation of endemic myxedematous cretinism and autoimmune thyroid disease...
  63. ncbi Effects of topical L-selenomethionine with topical and oral vitamin E on pigmentation and skin cancer induced by ultraviolet irradiation in Skh:2 hairless mice
    Karen E Burke
    Department of Biostatistics, University of Connecticut Health Center, Farmington, USA
    J Am Acad Dermatol 49:458-72. 2003
    ..The antioxidants selenium and vitamin E can be effective in reducing acute and chronic ultraviolet (UV)-induced skin damage...
  64. ncbi Bioavailability of selenium from raw or cured selenomethionine-enriched fillets of Atlantic salmon (Salmo salar) assessed in selenium-deficient rats
    Robin Ornsrud
    Directorate of Fisheries, Institute of Nutrition, Bergen, Norway
    Br J Nutr 87:13-20. 2002
    The bioavailability of Se from raw and cured selenomethionine-enriched (Se-enriched) salmon fillets was assessed in Se-deficient male albino rats (Mol: Wist)...
  65. ncbi Speciation analysis of selenium metabolites in urine and breath by HPLC- and GC-inductively coupled plasma-MS after administration of selenomethionine and methylselenocysteine to rats
    Yuki Ohta
    Graduate School of Pharmaceutical Sciences, Chiba University, Chuo, Chiba 260 8675 Japan
    Chem Res Toxicol 22:1795-801. 2009
    Selenium is an essential trace element found in vegetables as selenomethionine (SeMet) and methylselenocysteine (MeSeCys)...
  66. ncbi Selenium metabolites in urine of cancer patients receiving L-selenomethionine at high doses
    Doris Kuehnelt
    Institute of Chemistry Analytical Chemistry, Karl Franzens University Graz, Universitaetsplatz 1, 8010 Graz, Austria
    Toxicol Appl Pharmacol 220:211-5. 2007
    ..HPLC/mass spectrometry, the selenium metabolites in urine from five cancer patients receiving high doses of L-selenomethionine over an extended period (2 x 4000 microg Se/day for 7 days, then 4000 microg Se/day for 21 days) as an ..
  67. ncbi The organic selenium compound selenomethionine modulates bleomycin-induced DNA damage and repair in human leukocytes
    Blanca Laffon
    Toxicology Unit, Department of Psychobiology, University of A Coruna, Edificio de Servicios Centrales de Investigación, Campus Elviña s n, 15071, A Coruna, Spain
    Biol Trace Elem Res 133:12-9. 2010
    The objective of this work was to evaluate the effects of selenomethionine (SeMet) on the induction, repair, and persistence of DNA damage in human leukocytes challenged with bleomycin (BLM)...
  68. ncbi Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system
    Ghader Bashiri
    Maurice Wilkins Center for Molecular Biodiscovery, Laboratory of Structural Biology, School of Biological Sciences, University of Auckland, Private Bag 92019, New Zealand
    Protein Expr Purif 54:38-44. 2007
    ..Both native and selenomethionine-substituted FGD1 were obtained by culturing M...
  69. ncbi Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination
    Lucy Stols
    Environmental Research Division, Argonne National Laboratory, Argonne, IL 60439, USA
    J Struct Funct Genomics 5:95-102. 2004
    ..that the approach is also well suited for production of proteins in defined media with incorporation of selenomethionine to facilitate structure determination by multiwavelength anomalous diffraction...
  70. pmc Crystal structure of Bfr A from Mycobacterium tuberculosis: incorporation of selenomethionine results in cleavage and demetallation of haem
    Vibha Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi, India
    PLoS ONE 4:e8028. 2009
    ..In addition, this study provides evidence against the general belief that a selenium derivative of a protein represents its true physiological native structure...
  71. ncbi Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells
    Norio Yamamoto
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Cancer Gene Ther 10:445-50. 2003
    We have previously shown that the toxic pro-oxidant methylselenol is released from selenomethionine (SeMET) by cancer cells transformed with the adenoviral methionine alpha,gamma-lyase (methioninase, MET) gene cloned from Pseudomonas ..
  72. pmc Apoptosis induced by selenomethionine and methioninase is superoxide mediated and p53 dependent in human prostate cancer cells
    Rui Zhao
    Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4 868, Clinical Science Center, Box 8550, 600 Highland Avenue, Madison, WI 53792, USA
    Mol Cancer Ther 5:3275-84. 2006
    b>Selenomethionine (SeMet) is the chemical form or major component of selenium used for cancer chemoprevention in several clinical trials...
  73. ncbi The in vitro effects of selenomethionine on methylmercury-induced neurotoxicity
    Parvinder Kaur
    Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Olav Kyrresgt 3, N 7489 Trondheim, Norway
    Toxicol In Vitro 23:378-85. 2009
    ..Therefore, we investigated whether 24h. preincubation or 50min. coincubation with selenomethionine (SeMet) was effective in reducing methylmercury (MeHg)-induced cytotoxicity in C6-glioma and B35-neuronal ..
  74. ncbi Oxidation of selenomethionine: some MADness in the method!
    A J Sharff
    Department of Biochemistry, University of Cambridge, England
    Acta Crystallogr D Biol Crystallogr 56:785-8. 2000
    ..Here, it is demonstrated that the anomalous signal from selenomethione (SeMet) substituted proteins can be significantly enhanced by oxidation...
  75. ncbi Selenium and selenomethionine levels in prostate cancer patients
    David W Nyman
    Department of Pharmacology Toxicology, University of Arizona Health Sciences Center, Tucson, AZ 85721 0207, USA
    Cancer Detect Prev 28:8-16. 2004
    Selenium (Se) and selenomethionine (Se-Met) have been identified as potential chemopreventive agents of prostate cancer...
  76. ncbi Biomarkers of exposure and effect as indicators of the interference of selenomethionine on methylmercury toxicity
    Ana P M dos Santos
    Centro de Estudos de Ciências Farmacêuticas, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Toxicol Lett 169:121-8. 2007
    The present study was conducted to clarify the interference of selenomethionine (SeMet) on methylmercury (MeHg) toxicity through the evaluation of changes in biomarkers of exposure and effect in rats exposed to MeHg and co-exposed to MeHg ..
  77. ncbi Profiling of selenomethionine responsive genes in colon cancer by microarray analysis
    Anne Christine Goulet
    Department of Pathology, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA
    Cancer Biol Ther 6:494-503. 2007
    ..Previous studies by our group demonstrated that selenomethionine-induced growth arrest appears to be mediated by activation of ERK and subsequent phosphorylation of RSK and ..
  78. pmc Reduction of L-methionine selenoxide to seleno-L-methionine by endogenous thiols, ascorbic acid, or methimazole
    Renee J Krause
    Department of Comparative Biosciences and the Center for Molecular and Environmental Toxicology, University of Wisconsin Madison, Madison, WI 53706, United States
    Biochem Pharmacol 77:134-40. 2009
    ..Furthermore, the novel reduction of MetSeO by methimazole provides clear evidence that methimazole should not be used as an alternative FMO substrate when studying FMO-mediated oxidation of SeMet...
  79. ncbi Selenite or selenomethionine interaction with methylmercury on uptake and toxicity showing a weak selenite protection: studies on cultured K-562 cells
    P Frisk
    Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences, Uppsala University, Sweden
    Biol Trace Elem Res 80:251-68. 2001
    ..Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 microM) or selenomethionine (10 or 50 microM) together with (3.5 or 5 microM) MMC...
  80. ncbi A phase I and pharmacokinetic study of fixed-dose selenomethionine and irinotecan in solid tumors
    Marwan G Fakih
    Department of Medicine, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Clin Cancer Res 12:1237-44. 2006
    We conducted a phase I study to determine the maximum tolerated dose (MTD) of irinotecan with fixed, nontoxic high dose of selenomethionine.
  81. ncbi Selenomethionine stimulates expression of glutathione peroxidase 1 and 3 and growth of bovine mammary epithelial cells in primary culture
    S G Miranda
    Department of Animal Science, University of Zulia, Maracaibo, Venezuela 4005
    J Dairy Sci 92:2670-83. 2009
    ..The effect of selenium as selenomethionine (SeMet) on the growth and viability of BMEC and GPx protein expression and activity were also studied...
  82. ncbi Selenium compounds regulate p53 by common and distinctive mechanisms
    Martin L Smith
    Indiana University School of Medicine, Department of Microbiology and Walther Oncology Center, Indianapolis, IN 46202, USA
    Anticancer Res 24:1401-8. 2004
    ..The main dietary form is selenomethionine, which we showed modulated p53 activity by causing redox regulation of key p53 cysteine residues...
  83. ncbi Randomized, placebo-controlled, esophageal squamous cell cancer chemoprevention trial of selenomethionine and celecoxib
    Paul J Limburg
    Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Gastroenterology 129:863-73. 2005
    ..Squamous dysplasia, the accepted histological precursor for esophageal squamous cell carcinoma, represents a potentially modifiable intermediate end point for chemoprevention trials in high-risk populations...
  84. ncbi Selenomethionine regulates cyclooxygenase-2 (COX-2) expression through nuclear factor-kappa B (NF-kappaB) in colon cancer cells
    Durga P Cherukuri
    Department of Pathology, Arizona Cancer Center, The University of Arizona College of Medicine, Tucson, Arizona 85724, USA
    Cancer Biol Ther 4:175-80. 2005
    Previously, we showed that selenomethionine (Se-Met) inhibits growth of colon cancer cells via suppressing COX-2 expression at both mRNA and protein level...
  85. ncbi Changes in serum proteomic patterns by presurgical alpha-tocopherol and L-selenomethionine supplementation in prostate cancer
    Jeri Kim
    Department of Genitourinary Medical Oncology, University of Massachusetts Medical School, Lazare Research Building, Room 504, 364 Plantation Street, Worcester, MA 01605 2324, USA
    Cancer Epidemiol Biomarkers Prev 14:1697-702. 2005
    Evidence of the chemopreventive effects of the dietary antioxidants alpha-tocopherol (vitamin E) and l-selenomethionine (selenium) comes from secondary analysis of two phase III clinical trials that found treatment with these antioxidants ..
  86. ncbi Production of selenomethionine-labelled proteins using simplified culture conditions and generally applicable host/vector systems
    S A Guerrero
    , Braunschweig, Germany
    Appl Microbiol Biotechnol 56:718-23. 2001
    The amino acid analogue selenomethionine (SeMet) is shown to be efficiently incorporated into recombinant proteins expressed in Escherichia coli grown in a simple minimal medium without the addition of synthetic amino acids...
  87. ncbi Incorporation of selenomethionine into induced intracytoplasmic membrane proteins of Rhodobacter species
    Philip D Laible
    Biosciences Division, Argonne National Laboratory, 9700 S Cass Avenue, Argonne, IL 60439, USA
    J Struct Funct Genomics 6:95-102. 2005
    ..Development of methods for the in vivo substitution of methionine by selenomethionine (SeMet) has revolutionized the process for determination of structures of soluble proteins in recent years...
  88. ncbi Methioninase and selenomethionine but not Se-methylselenocysteine generate methylselenol and superoxide in an in vitro chemiluminescent assay: implications for the nutritional carcinostatic activity of selenoamino acids
    Julian E Spallholz
    Food and Nutrition, College of Human Sciences, Texas Tech University, Lubbock, TX 79409, USA
    Biochem Pharmacol 67:547-54. 2004
    ..methylselenol-generating chemiluminesence by superoxide (O2*-) is shown to be catalytically produced from L-selenomethionine and D,L-selenoethionine, but not from methionine or L-Se-methylselenocysteine (SeMC)...
  89. pmc Selenomethionine incorporation in proteins expressed in Lactococcus lactis
    Ronnie P A Berntsson
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Protein Sci 18:1121-7. 2009
    ..Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis...
  90. pmc Effects of selenomethionine supplementation on selenium status and thyroid hormone concentrations in healthy adults
    Gerald F Combs
    Grand Forks Human Nutrition Research Center, US Department of Agriculture, Agricultural Research Service, Grand Forks, ND 55202, USA
    Am J Clin Nutr 89:1808-14. 2009
    ..The effects of long-term selenium supplementation on thyroid hormone concentrations are unknown...
  91. ncbi The effect of selenium, as selenomethionine, on genome stability and cytotoxicity in human lymphocytes measured using the cytokinesis-block micronucleus cytome assay
    Jing Wu
    Nutrigenomics Laboratory, CSIRO Human Nutrition, SA, Australia
    Mutagenesis 24:225-32. 2009
    ..Therefore, a cautious approach to supplementation with Se-met is required to ensure that optimal genome health is achieved without cytotoxic effects...
  92. pmc Reduction of gene repair by selenomethionine with the use of single-stranded oligonucleotides
    Timothy R Schwartz
    Department of Biological Sciences, University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way Newark, DE 19711, USA
    BMC Mol Biol 8:7. 2007
    ..This process is regulated by the activity of various factors and proteins that either elevate or depress the frequency at which gene repair takes place...
  93. ncbi Selenomethionine induces sustained ERK phosphorylation leading to cell-cycle arrest in human colon cancer cells
    Anne Christine Goulet
    Department of Pathology, Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, USA
    Carcinogenesis 26:109-17. 2005
    b>Selenomethionine (SeMet) is being tested alone and in combination with other agents in cancer chemoprevention trials...
  94. pmc XANES measurements of the rate of radiation damage to selenomethionine side chains
    James M Holton
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158 2330, USA
    J Synchrotron Radiat 14:51-72. 2007
    The radiation-induced disordering of selenomethionine (SeMet) side chains represents a significant impediment to protein structure solution...
  95. ncbi Selenomethionine inhibits ultraviolet radiation-induced p53 transactivation
    Nicola J Traynor
    Photobiology Unit, Ninewells Hospital, University of Dundee, Dundee, UK
    Photodermatol Photoimmunol Photomed 22:297-303. 2006
    ....
  96. ncbi Selenomethionine inhibits growth and suppresses cyclooxygenase-2 (COX-2) protein expression in human colon cancer cell lines
    Antonio Baines
    Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA
    Cancer Biol Ther 1:370-4. 2002
    Currently, selenium (in the form of high selenium containing yeast or selenomethionine) is being evaluated for anticancer effects against both human colon polyp recurrence and human prostate cancer, respectively...
  97. pmc An essential role of s-adenosyl-L-methionine:L-methionine s-methyltransferase in selenium volatilization by plants. Methylation of selenomethionine to selenium-methyl-L-selenium- methionine, the precursor of volatile selenium
    Abderrahmane Tagmount
    Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720 3102, USA
    Plant Physiol 130:847-56. 2002
    ..Thus, our results provide in vivo evidence that MMT is the key enzyme catalyzing the methylation of Se-Met to SeMM...
  98. ncbi Selenomethionine labeling of recombinant proteins
    Anna M Larsson
    Department of Cell and Molecular Biology, University of Uppsala, Uppsala, Sweden
    Methods Mol Biol 389:165-74. 2007
    b>Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion...
  99. ncbi A Phase I and pharmacokinetic study of selenomethionine in combination with a fixed dose of irinotecan in solid tumors
    Marwan G Fakih
    Department of Medicine, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Cancer Chemother Pharmacol 62:499-508. 2008
    We conducted a phase I study to determine the recommended dose of selenomethionine (SLM) in combination with irinotecan that consistently results in a protective plasma selenium (Se) concentrations > 15 microM after 1 week of SLM ..
  100. ncbi Selenium (Se) from high-selenium broccoli is utilized differently than selenite, selenate and selenomethionine, but is more effective in inhibiting colon carcinogenesis
    J W Finley
    United States Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, ND 58202, USA
    Biofactors 14:191-6. 2001
    ..amounts of Se from high-Se processed wheat also significantly reduced aberrant crypts (AC), although pure selenomethionine, (the predominant chemical form of Se in wheat), did not significantly reduce AC...
  101. ncbi Comparative toxicosis of sodium selenite and selenomethionine in lambs
    Asheesh K Tiwary
    California Animal Health and Food Safety Laboratory System, University of California, Davis, USA
    J Vet Diagn Invest 18:61-70. 2006
    ..The objective of the study reported here was to compare the acute toxicosis caused by organic selenium (selenomethionine) found in plants with that caused by the supplemental, inorganic form of selenium (sodium selenite)...

Research Grants63

  1. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven A Belinsky; Fiscal Year: 2012
    ..This trial is testing the hypothesis that 200 5g of L-selenomethionine given as selenized yeast can decrease the rate of second primary tumors in patients who have undergone ..
  2. The influence of selenium on biomarkers of prostate cancer risk
    KARAM E EL-BAYOUMY; Fiscal Year: 2012
    ..a double-blind, randomized, placebo-controlled clinical trial of selenium supplementation in the form of selenomethionine (SM=200 5g/day) and SY (240 5g/day and 350 5g/day the higher dose will deliver 200 5g/day of SM;(the ..
  3. Structural studies of PKR regulation by viral non-coding RNA
    GRAEME LESLIE CONN; Fiscal Year: 2013
    ..Alternatively, selenomethionine incorporation into protein components of the complex will be used to obtain experimental phases to determine ..
  4. Colorectal Neoplasia in SELECT Participants
    M PETER LANCE; Fiscal Year: 2011
    ..The SELECT Trial will assess the effect of selenium, as L-selenomethionine, and vitamin E alone and in combination on the clinical incidence of prostate cancer in 35,584 now-randomized ..
  5. Chemoprevention of arsenic-induced skin cancer
    Habibul Ahsan; Fiscal Year: 2013
    ..4,444 individuals with arsenic-related skin lesions into 4 treatment arms: vitamin E only (100 IU/day), L-selenomethionine only (200 5g/day), both vitamin E and selenium, and placebo...
  6. Prevention of PTEN deletion driven prostate cancer by selenium
    Junxuan Lu; Fiscal Year: 2012
    ..Trial (SELECT) in October 2008 ahead of schedule because of the failure to demonstrate an efficacy of selenomethionine (SeMet) for PCa prevention in North American men and a slight but non- statistically significant increase in ..
  7. Investigations of Bacterial Biofilm Proteomes Using Artifical Amino Acids
    NICHOLAS DEWAYNE BALL; Fiscal Year: 2013
    ..To address this issue we propose using L-selenomethionine (SeMet) as a surrogate amino acid for protein labeling due to its near complete incorporation into proteins ..
  8. Supplemental Se and Vitamin E and Pulmonary Function
    PATRICIA CASSANO; Fiscal Year: 2007
    ..men testing whether daily supplementation with vitamin E (400mg alpha-tocopherol), selenium (200 micrograms selenomethionine) or both vitamin E and selenium can prevent prostate cancer...
  9. Differential Gene Expression Induced by Antioxidants
    Jeri Kim; Fiscal Year: 2004
    ..The aims of this study are: 1) to identify genes differentially induced by the antioxidants l-selenomethionine (selenium) and alpha-tocopherol (vitamin E), singly and in combination, in normal and cancerous prostate ..
  10. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2006
    ..of DNA lesions in single MN and to determine if known and potentially new pharmacotherapies (creatine and selenomethionine) for mouse ALS attenuate the formation of DNA lesions...
  11. STRUCTURE AND ASSEMBLY OF THE HIV1 CAPSID PROTEIN
    Christopher Hill; Fiscal Year: 2001
    ..363, and grown crystals of both the native and selenomethionine- substituted proteins. Very recently, we have collected complete data sets at 2...
  12. A new method for delivery of selinium for prevention of melanoma
    Pamela Cassidy; Fiscal Year: 2007
    ..Aim 2: Evaluate new formulations of Se- methylselenocysteine (SeMSec) and selenomethionine (SeMet) for the delivery of selenium to the skin of mice...
  13. Genetic Analysis of Selenium Toxicity
    Annette Estevez; Fiscal Year: 2005
    ..In Caenorhabditis elegans exposure to selenomethionine (SeMet) causes progressive paralysis, decreased fertility, and growth retardation of adults derived from ..
  14. X RAY STRUCTURE OF UDP GALACTOPYRANOSE MUTASE
    Michael McNeil; Fiscal Year: 2000
    ..0, beta = 96.38_). Dr. Naismith's group has identified a platinum derivative of the E. coli enzyme using heavy metal soaking experiments and crystallized selenomethionine E. coli UDP-galactopyranose mutase.
  15. Development of selenomethionine resistant yeast
    TERRI KINZY; Fiscal Year: 2007
    ..In this aspect, one of the most important techniques now routinely used is the production of selenomethionine (SeMet) substituted recombinant proteins...
  16. SYNERGISTIC METHIONINASE GENE THERAPY OF CANCER
    Yuying Tan; Fiscal Year: 2000
    ..either: 1) the recombinant methioninase protein (rMETase), which itself has selective antitumor efficacy; 2) selenomethionine (SeMeth) as a prodrug analog cleaved by the METase gene product to toxic methylselenol...
  17. MHCL RESTRICTED TCR RECOGNITION--STRUCTURAL BASIS
    Jia huai Wang; Fiscal Year: 2002
    ..8 A resolution by molecular replacement in conjunction with selenomethionine/MAD phasing...
  18. SELENIUM SUPPLEMENTATION OF PATIENTS WITH CIRRHOSIS
    Raymond Burk; Fiscal Year: 2000
    ..Subjects in the groups will receive, respectively, 200 mug selenium as selenate, 200 mug selenium as selenomethionine, or placebo in a double blind fashion...
  19. STRUCTURAL BASIS OF GANGLIOSIDE RECOGNITION
    Christine Wright; Fiscal Year: 2003
    ..The crystal structure of the selenomethionine mutant of the GM2-AP has been solved at 2.4 A by multiwavelength anomalous diffraction...
  20. ASSESSMENT OF SELENIUM STATUS IN MAN
    Morteza Janghorbani; Fiscal Year: 1990
    ..b>selenomethionine) on the validity of the method...
  21. SELENIUM--ANTIOXIDANT OR PROOXIDANT MECHANISM
    Barbara Pence; Fiscal Year: 2001
    ..A recent intervention trial in human skin cancer patients demonstrated no efficacy of selenomethionine against the recurrence of basal cell and squamous cell carcinomas, although there was a decrease in incidence ..
  22. Structures of Bacterial Membrane-Bound Oxidoreductases
    Joel Weiner; Fiscal Year: 2006
    ..9Angstrom resolution and selenomethionine labeled protein crystals, (ii) to develop improved over-expression and purification protocols for DmsABC to ..
  23. Photoprotection of Skin by Topical Selenium
    MOSTAFA OMAR; Fiscal Year: 2002
    ..Formulations - including selenium sulfite, L-selenomethionine, selenotrisulfide, derivatives of lipoic acid and lipoamide - will be tested for percutaneous absorption in ..
  24. Structure and Diversity of N-Acyl Amino Acid Synthases
    RYAN VAN WAGONER; Fiscal Year: 2004
    ..coli. 2-A high-resolution model of the enzyme will be determined through X- ray crystallography on a selenomethionine-containing derivative of an N-aceyl amino acid synthase...
  25. STRUCTURAL ORGANIZATION AND PROTEOMICS OF TB
    Thomas Terwilliger; Fiscal Year: 2005
    ..b>Selenomethionine MAD X-ray data collection on characterized crystals at synchrotron facilities will be emphasized, with ..
  26. A FEASIBILITY STUDY OF L-SELENOMETHIONINE AND ALPHA-TOCO
    Jeri Kim; Fiscal Year: 2001
    ..To these ends, we will conduct a randomized, placebo-controlled short-term trial of d- alpha-tocopherol, 1-selenomethionine and the combination of d- alpha-tocopherol plus 1-selenomenthionine in presurgical cohorts with clinically ..
  27. Structure and Mechanism of C1pB from E. coli
    BRITTNAIE BELL; Fiscal Year: 2005
    ..the classical multiple isomorphous replacent method or by multiwavelength anomalous diffraction (MAD) of the selenomethionine form of the enzyme...
  28. Structural Studies of NAGS
    Dashuang Shi; Fiscal Year: 2006
    ..abstract_text> ..
  29. Selenium Based Chemoprevention - HGPIN
    James Marshall; Fiscal Year: 2008
    ..This study promises to be the largest and most methodologically rigorous to date of HGPIN, which is widely believed to be the key premalignant lesion for prostate cancer. ..
  30. Clinical Trial of Selenium and Prostate Biomarkers
    James Marshall; Fiscal Year: 2010
    ..scheduled for brachythrapy and prostatectomy to pretreatment supplementation by daily dose of 400 meg selenomethionine, or to placebo...
  31. NUTRITIONAL PREVENTION OF CANCER
    James Marshall; Fiscal Year: 2003
    ..The proposed project will complete the data collection for the NPC trial and will utilize this invaluable resource to verify the effects of selenium supplementation through a series of publications. ..
  32. Selenium molecular signaling in human prostate cancer
    Clement Ip; Fiscal Year: 2007
    ..Aim 4 is to study the transcriptional control of GADD153 by selenium. The proposed research is thus designed to examine systemically the impact of GADD153 induction as a result of selenium treatment. ..
  33. MAMMARY CANCER PREVENTION BY CONJUGATED LINOLEIC ACID
    Clement Ip; Fiscal Year: 2007
    ..abstract_text> ..
  34. Selenium protection of bone marrow during chemotherapy
    Martin Smith; Fiscal Year: 2009
    ..These studies will provide a molecular mechanism for bone marrow protection by selenium in combination with cancer chemotherapeutics, and will foster further clinical studies. ..
  35. Molecular biomarkers of selenium chemoprevention
    Clement Ip; Fiscal Year: 2006
    ..The goal is to broaden our understanding of the significance of clonal suppression of early transformed cells in selenium protection of cancer. ..
  36. WHAT IS THE METABOLIC FUNCTION OF SELENOPROTEIN W?
    PHILIP WHANGER; Fiscal Year: 2003
    ..These animals will also be supplemented with selenium to determine the effects upon selenoprotein W and GPX1 and GPX4 induction. ..
  37. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  38. CANCER PREVENTION BY SELENIUM-ENRICHED ALLIUM VEGETABLES
    Clement Ip; Fiscal Year: 2003
    ..The objective is to corroborate the significance of clonal suppression of preneoplastic lesions in selenium protection of cancer. ..
  39. Targets for selenium in prostate cancer prevention
    JOHN PINTO; Fiscal Year: 2009
    ..identified in this study will provide relevant biomarkers useful for the on-going SELECT Study that examines selenomethionine for prevention of CaP...
  40. ROLE OF THE P34CDC2 RELATED PITSLRE PROTEIN KINASES
    Mark Nelson; Fiscal Year: 2007
    ..The greatest impact will be realized in the provision of novel molecular targets for therapeutic intervention strategies. ..
  41. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  42. Molecular Epidemiology of Colorectal Cancer Subtypes
    Paul J Limburg; Fiscal Year: 2010
    ....
  43. Effects of HAART drugs on endothelial dysfunction of pu*
    Changyi Chen; Fiscal Year: 2009
    ..abstract_text> ..
  44. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond F Burk; Fiscal Year: 2010
    ..An equation was derived that related increase in plasma selenium concentration to selenomethionine intake. The third study showed that some patients in the U.S...
  45. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  46. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  47. HIV/SIV ENVELOPE GLYCOPROTEIN EFFECTS ON ENDOTHELIA
    Changyi Chen; Fiscal Year: 2002
    ....
  48. Allium Compounds in Control of Human Prostate Cancer
    JOHN PINTO; Fiscal Year: 2003
    ....
  49. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2003
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  50. In Vitro Cancer Prevention Biomarker Development
    Eugene Elmore; Fiscal Year: 2004
    ..Using cluster analysis, we will determine the global gene expression changes induced by each agent. The strength of the proposed approach lies in the high quality data that has already determined from the HEC Assay. ..
  51. HIV PROTEASE INHIBITORS EFFECT--NENDOTHELIAL DYSFUNCTION
    Changyi Chen; Fiscal Year: 2004
    ..Together, the integrated basic science, non-human primate and human investigations offer a multi disciplinary approach to the understanding and prevention of protease inhibitor-associated vascular complications. ..
  52. Neurobehavioral Development in Mercury-Exposed Zebrafish
    Daniel Weber; Fiscal Year: 2005
    ..exposure through continual yolk absorption of maternally-derived Hg. This study will mirror prospective studies on human populations over much shorter time periods. ..
  53. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  54. Selenium Prevention of Tobacco Smoke-Induced Lung Cancer
    Teresa Fan; Fiscal Year: 2005
    ..1) To examine whether dietary Se supplement in the form of selenized yeast (Se-yeast) or its major component selenomethionine (Se-Met) is chemopreventive for mice that have "quit" smoking; 2) To characterize biomarkers of Se action ..
  55. Effects of HIV Protease Inhibitors of Endothelial Barrie
    Changyi Chen; Fiscal Year: 2005
    ..Our findings may also suggest new therapeutic strategies, which may eventually prove effective for the prevention and treatment of these complications. ..
  56. Visualizing Clonal Expansion in Living Mice
    Douglas Brash; Fiscal Year: 2005
    ..abstract_text> ..
  57. Insulin, the IGF Axis, and Colorectal Neoplasia
    Paul Limburg; Fiscal Year: 2005
    ..Following achievement of these goals, I am confident that I will be ready to continue my career as a successful independent investigator committed to academic research. ..
  58. Molecular Surgeon Symposium on Vascular Injury, Repair
    Changyi Chen; Fiscal Year: 2005
    ..A pre-approved letter for acceptance of submission of this application from NIH/NHLBI is included. ..
  59. Mechanistic Effects of Organic Selenium Against Colon C
    Mark Nelson; Fiscal Year: 2006
    ..However, the mechanism by which organic selenium exerts its anticancer effects remains to be identified. Selenomethionine is the predominant form of selenium in the yeast...
  60. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  61. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  62. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..