selenomethionine

Summary

Summary: Diagnostic aid in pancreas function determination.

Top Publications

  1. ncbi Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice
    Huali Wang
    University of Science and Technology of China, Hefei 230052, People s Republic of China
    Free Radic Biol Med 42:1524-33. 2007
  2. ncbi Selenite and selenomethionine promote HL-60 cell cycle progression
    Huawei Zeng
    U S Department of Agriculture, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202 9034, USA
    J Nutr 132:674-9. 2002
  3. doi Dietary selenomethionine increases exon-specific DNA methylation of the p53 gene in rat liver and colon mucosa
    Huawei Zeng
    USDA, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, USA
    J Nutr 141:1464-8. 2011
  4. doi Differential responses to selenomethionine supplementation by sex and genotype in healthy adults
    Gerald F Combs
    Grand Forks Human Nutrition Research Center, USDA ARS, 2420 2nd Avenue North, Stop 9034, Grand Forks, ND 58202 9034, USA
    Br J Nutr 107:1514-25. 2012
  5. pmc Superior in vivo inhibitory efficacy of methylseleninic acid against human prostate cancer over selenomethionine or selenite
    Guang Xun Li
    Hormel Institute, University of Minnesota, 801 16th Avenue Northeast, Austin, MN 55912, USA
    Carcinogenesis 29:1005-12. 2008
  6. doi Selenium and the thyroid: a close-knit connection
    Leonidas H Duntas
    Endocrine Unit, Evgenidion Hospital, University of Athens, 20 Papadiamantopoulou Street 115 28 Athens, Greece
    J Clin Endocrinol Metab 95:5180-8. 2010
  7. pmc Selenomethionine regulation of p53 by a ref1-dependent redox mechanism
    Young R Seo
    Department of Microbiology, Walther Oncology Center, and Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, IN 46208, USA
    Proc Natl Acad Sci U S A 99:14548-53. 2002
  8. ncbi Selenium accumulation in prostate tissue during a randomized, controlled short-term trial of l-selenomethionine: a Southwest Oncology Group Study
    Anita L Sabichi
    University of Texas M D Anderson Cancer Center, Baylor College of Medicine, Houston, Texas, USA
    Clin Cancer Res 12:2178-84. 2006
  9. ncbi Methylselenol generated from selenomethionine by methioninase downregulates integrin expression and induces caspase-mediated apoptosis of B16F10 melanoma cells
    Aeyung Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
    J Cell Physiol 212:386-400. 2007
  10. doi Speciation analysis of selenium metabolites in urine and breath by HPLC- and GC-inductively coupled plasma-MS after administration of selenomethionine and methylselenocysteine to rats
    Yuki Ohta
    Graduate School of Pharmaceutical Sciences, Chiba University, Chuo, Chiba 260 8675 Japan
    Chem Res Toxicol 22:1795-801. 2009

Detail Information

Publications257 found, 100 shown here

  1. ncbi Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice
    Huali Wang
    University of Science and Technology of China, Hefei 230052, People s Republic of China
    Free Radic Biol Med 42:1524-33. 2007
    ..b>Selenomethionine is considered to be the most appropriate supplemental form due to its excellent bioavailability and lower ..
  2. ncbi Selenite and selenomethionine promote HL-60 cell cycle progression
    Huawei Zeng
    U S Department of Agriculture, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202 9034, USA
    J Nutr 132:674-9. 2002
    ..In this study, the effects of selenium deficiency or adequacy (0.25 micromol/L selenite or selenomethionine) on HL-60 cell cycle progression were examined in serum-free media...
  3. doi Dietary selenomethionine increases exon-specific DNA methylation of the p53 gene in rat liver and colon mucosa
    Huawei Zeng
    USDA, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, USA
    J Nutr 141:1464-8. 2011
    ..15 [Se adequate (A)], or 4 mg [Se supranutritional (S)] (Se as l-selenomethionine)/kg diet for 104 d, respectively...
  4. doi Differential responses to selenomethionine supplementation by sex and genotype in healthy adults
    Gerald F Combs
    Grand Forks Human Nutrition Research Center, USDA ARS, 2420 2nd Avenue North, Stop 9034, Grand Forks, ND 58202 9034, USA
    Br J Nutr 107:1514-25. 2012
    ..to characterise the responses of multiple biomarkers of Se status in healthy American adults to supplemental selenomethionine (SeMet) and to identify factors affecting those responses...
  5. pmc Superior in vivo inhibitory efficacy of methylseleninic acid against human prostate cancer over selenomethionine or selenite
    Guang Xun Li
    Hormel Institute, University of Minnesota, 801 16th Avenue Northeast, Austin, MN 55912, USA
    Carcinogenesis 29:1005-12. 2008
    ..methylselenol precursors methylseleninic acid (MSeA) and Se-methylselenocysteine (MSeC) in comparison with selenomethionine (SeMet) and selenite in DU145 and PC-3 human PCa xenografts in athymic nude mice...
  6. doi Selenium and the thyroid: a close-knit connection
    Leonidas H Duntas
    Endocrine Unit, Evgenidion Hospital, University of Athens, 20 Papadiamantopoulou Street 115 28 Athens, Greece
    J Clin Endocrinol Metab 95:5180-8. 2010
    ..It has additionally been established that the thyroid contains more selenium than any other tissue and that selenium deficiency aggravates the manifestation of endemic myxedematous cretinism and autoimmune thyroid disease...
  7. pmc Selenomethionine regulation of p53 by a ref1-dependent redox mechanism
    Young R Seo
    Department of Microbiology, Walther Oncology Center, and Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, IN 46208, USA
    Proc Natl Acad Sci U S A 99:14548-53. 2002
    ..We show that selenium in the form of selenomethionine (SeMet) can activate the p53 tumor suppressor protein by a redox mechanism that requires the redox factor ..
  8. ncbi Selenium accumulation in prostate tissue during a randomized, controlled short-term trial of l-selenomethionine: a Southwest Oncology Group Study
    Anita L Sabichi
    University of Texas M D Anderson Cancer Center, Baylor College of Medicine, Houston, Texas, USA
    Clin Cancer Res 12:2178-84. 2006
    ..Epidemiologic and clinical data suggest that selenium could prevent prostate cancer, but it has not been shown that supplemental selenium leads to an increased concentration of selenium in prostate tissue compared with adjacent tissue...
  9. ncbi Methylselenol generated from selenomethionine by methioninase downregulates integrin expression and induces caspase-mediated apoptosis of B16F10 melanoma cells
    Aeyung Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea
    J Cell Physiol 212:386-400. 2007
    ..In this study, we examine the effects of methylselenol generated from selenomethionine (SeMet) by methioninase (METase) on cell proliferation, adhesion, and expression of integrins in murine ..
  10. doi Speciation analysis of selenium metabolites in urine and breath by HPLC- and GC-inductively coupled plasma-MS after administration of selenomethionine and methylselenocysteine to rats
    Yuki Ohta
    Graduate School of Pharmaceutical Sciences, Chiba University, Chuo, Chiba 260 8675 Japan
    Chem Res Toxicol 22:1795-801. 2009
    Selenium is an essential trace element found in vegetables as selenomethionine (SeMet) and methylselenocysteine (MeSeCys)...
  11. pmc Optimization of selenoprotein P and other plasma selenium biomarkers for the assessment of the selenium nutritional requirement: a placebo-controlled, double-blind study of selenomethionine supplementation in selenium-deficient Chinese subjects
    Yiming Xia
    Sichuan Provincial Center for Disease Control and Prevention, Chengdu, China
    Am J Clin Nutr 92:525-31. 2010
    ..The intake of selenium needed for optimal health has not been established. Selenoproteins perform the functions of selenium, and the selenium intake needed for their full expression is not known...
  12. ncbi Speciation and bioavailability of selenium in yeast-based intervention agents used in cancer chemoprevention studies
    Erik H Larsen
    Danish Institute for Food and Veterinary Research, Department of Food Chemistry, 19 Mørkhøj Bygade, DK 2860 Søborg, Denmark
    J AOAC Int 87:225-32. 2004
    ..The results for the NPC tablets showed that selenomethionine, together with 3 unidentified selenium compounds, were predominant in the sample hydrolysates...
  13. ncbi Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system
    Ghader Bashiri
    Maurice Wilkins Center for Molecular Biodiscovery, Laboratory of Structural Biology, School of Biological Sciences, University of Auckland, Private Bag 92019, New Zealand
    Protein Expr Purif 54:38-44. 2007
    ..Both native and selenomethionine-substituted FGD1 were obtained by culturing M...
  14. pmc Analysis of methionine/selenomethionine oxidation and methionine sulfoxide reductase function using methionine-rich proteins and antibodies against their oxidized forms
    Dung Tien Le
    Department of Biochemistry and Redox Biology Center, University of Nebraska, Lincoln, Nebraska 68588, USA
    Biochemistry 47:6685-94. 2008
    ..Furthermore, we prepared a selenomethionine form of an MRP and found that selenomethionine selenoxide residues can be efficiently reduced ..
  15. doi A novel method for detection of selenomethionine incorporation in protein crystals via Raman microscopy
    Alessandro Vergara
    Dipartimento di Chimica, Universita degli Studi di Napoli Federico II, Complesso Monte S Angelo, Via Cinthia, I 80126 Napoli, Italy
    Acta Crystallogr D Biol Crystallogr 64:167-71. 2008
    ..Mass spectrometry is currently used to evaluate the selenomethionine (SeMet) content in solution, but excluding fluorescence spectroscopy at the absorption edge, no other routine ..
  16. ncbi Contribution of enzymic alpha, gamma-elimination reaction in detoxification pathway of selenomethionine in mouse liver
    T Okuno
    Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge cho, Hirakata, Osaka 573 0101, Japan
    Toxicol Appl Pharmacol 176:18-23. 2001
    The objective of this study was to clarify the detoxification pathways of selenomethionine (SeMet) in mouse liver. It has been postulated that SeMet may be metabolized to selenocysteine (SeCyH) via a pathway similar to methionine (Met)...
  17. pmc A human model of selenium that integrates metabolism from selenite and selenomethionine
    Meryl E Wastney
    Metabolic Modeling Services Ltd, 7201 Blenheim, New Zealand
    J Nutr 141:708-17. 2011
    ..of this study was to simultaneously compare kinetics of absorbed Se from inorganic selenite (Sel) and organic selenomethionine (SeMet) in healthy participants (n = 31)...
  18. doi Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice
    Lin Yan
    U S Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202, USA
    Int J Cancer 131:1260-6. 2012
    ..investigated the effects of dietary supplementation with methylseleninic acid (MSeA), in comparison with selenomethionine (SeMet), on spontaneous metastasis of Lewis lung carcinoma (LLC) in male C57BL/6 mice using intramuscular and ..
  19. doi Rapamycin suppresses ROS-dependent apoptosis caused by selenomethionine in A549 lung carcinoma cells
    Maiko Suzuki
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Cancer Chemother Pharmacol 67:1129-36. 2011
    ..In this study, we investigated the effects of rapamycin on apoptosis induced by seleno-L-methionine (SeMet) or selenite in A549 cells...
  20. ncbi Sites and extent of selenomethionine incorporation into recombinant Cas6 protein by top-down and bottom-up proteomics with 14.5 T Fourier transform ion cyclotron resonance mass spectrometry
    Xu Wang
    Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, Florida 32306, USA
    Rapid Commun Mass Spectrom 24:2386-92. 2010
    b>Selenomethionine-modified proteins can improve X-ray crystallographic structural resolution by multi-wavelength anomalous diffraction (MAD) phasing...
  21. ncbi Non-optimal levels of dietary selenomethionine alter splenocyte response and modify oxidative stress markers in female mice
    Libia Vega
    Sección Externa de Toxicología, Centro de Investigacion y de Estudios Avanzados, Av IPN 2508, San Pedro Zacatenco, Mexico, DF 07360, Mexico
    Food Chem Toxicol 45:1147-53. 2007
    Many studies evaluating the effects of selenium (Se) status on immunity utilize inorganic Se, although selenomethionine (Se-Met) has been suggested to be more bioavailable and less toxic...
  22. pmc Prostatic response to supranutritional selenium supplementation: comparison of the target tissue potency of selenomethionine vs. selenium-yeast on markers of prostatic homeostasis
    David J Waters
    Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907, USA
    Nutrients 4:1650-63. 2012
    ..the Selenium and Vitamin E Cancer Prevention Trial (SELECT) showed no such cancer-protective advantage using selenomethionine (SeMet)...
  23. ncbi Methioninase cancer gene therapy with selenomethionine as suicide prodrug substrate
    K Miki
    AntiCancer Incorporated, San Diego, California 92111, USA
    Cancer Res 61:6805-10. 2001
    ..This gene therapy strategy exploits the toxic pro-oxidant property of methylselenol, which is released from selenomethionine (SeMET) by cancer cells with the adenoviral-delivered methionine alpha,gamma-lyase (MET) gene cloned from ..
  24. ncbi Selenomethionine induction of DNA repair response in human fibroblasts
    Young R Seo
    Indiana University Cancer Center, Department of Microbiology, Walther Oncology Center, Indiana University School of Medicine and Walther Cancer Institute, Indianapolis, IN 46202, USA
    Oncogene 21:3663-9. 2002
    ..We show that selenium in the form of selenomethionine induces a DNA repair response in normal human fibroblasts in vitro, and protects cells from DNA damage...
  25. pmc Selenomethionine reduces visual deficits due to developmental methylmercury exposures
    Daniel N Weber
    Marine and Freshwater Biomedical Sciences Center, University of Wisconsin Milwaukee, 600 E Greenfield Ave, Milwaukee, WI 53204, United States
    Physiol Behav 93:250-60. 2008
    ..0-0.30 microM MeHg and/or selenomethionine (SeMet) until 24 hpf then placed in clean medium...
  26. ncbi Selective labeling of selenomethionine residues in proteins with a fluorescent derivative of benzyl bromide
    Shenhui Lang
    Department of Chemistry, University of Waterloo, Waterloo, Ont, Canada N2L 3G1
    Anal Biochem 359:253-8. 2006
    ..of the bromobenzyl moiety as a reactive group for the targeted fluorescent labeling of methionine and selenomethionine residues in proteins...
  27. ncbi Dimethyldiselenide and methylseleninic acid generate superoxide in an in vitro chemiluminescence assay in the presence of glutathione: implications for the anticarcinogenic activity of L-selenomethionine and L-Se-methylselenocysteine
    J E Spallholz
    Department of Ophthalmology, Texas Tech University Health Sciences Center, Selenium Technologies, Inc, Lubbock, TX, USA
    Nutr Cancer 40:34-41. 2001
    The reduction of cancer incidence by dietary supplementation with L-selenomethionine, L-Se-methylselenocysteine, and other methylated selenium compounds and metabolites is believed to be due to the metabolic generation of the ..
  28. ncbi Metabolism of 76Se-methylselenocysteine compared with that of 77Se-selenomethionine and 82Se-selenite
    Kazuo T Suzuki
    Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260 8675, Japan
    Toxicol Appl Pharmacol 217:185-95. 2006
    Se-Methylated selenoamino acids, Se-methylselenocysteine (MeSeCys) and selenomethionine (SeMet), are chemically inert storage forms of selenium in selenium-accumulators, and a nutritional and supplemental source...
  29. ncbi Dietary supplementation of selenomethionine reduces metastasis of melanoma cells in mice
    L Yan
    Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, USA
    Anticancer Res 19:1337-42. 1999
    The present study investigated the effect of dietary supplementation of selenomethionine on pulmonary metastasis of B16BL6 murine melanoma cells in C57BL/6 mice. Mice were assigned to four groups of 15 each...
  30. ncbi Development of stable isotope and selenomethionine labeling methods for proteins expressed in Pseudomonas fluorescens
    Krishna Madduri
    Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, IN 46268, USA
    Protein Expr Purif 65:57-65. 2009
    ..in structure elucidation studies, the availability of target proteins labeled with stable isotopes or selenomethionine is essential for the determination of protein structures...
  31. doi Selenomethionine treatment in patients with autoimmune thyroiditis: a prospective, quasi-randomised trial
    A D Anastasilakis
    Department of Endocrinology, 424 Military Hospital, Thessaloniki, Greece
    Int J Clin Pract 66:378-83. 2012
    ..To test whether selenium administration affects autoantibodies to thyroid peroxidase (anti-TPO) and thyroglobulin (anti-TG) titres in chronic autoimmune (Hashimoto's - HT) thyroiditis...
  32. pmc Selenomethionine incorporation into amyloid sequences regulates fibrillogenesis and toxicity
    Javier Martinez
    Instituto de Quimica Fisica Rocasolano, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    PLoS ONE 6:e27999. 2011
    ..Some of the sequences undergoing fibrillation contain critical methionine (Met) residues which in vivo can be synthetically substituted by selenomethionine (SeM) and alter their properties.
  33. ncbi The anticancer effects of sodium selenite and selenomethionine on human colorectal carcinoma cell lines in nude mice
    Yang Yang
    National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, People s Republic of China
    Oncol Res 18:1-8. 2009
    ..to evaluate the chemotherapeutic potential of selenium containing compounds such as sodium selenite (SSe) and selenomethionine (SeMet). Three doses of anticancer drugs were used, including 0...
  34. pmc Interactions of Cu(I) with selenium-containing amino acids determined by NMR, XAS, and DFT studies
    Hsiao C Wang
    Department of Chemistry, Clemson University, Clemson, South Carolina 29634 0973, USA
    Inorg Chem 50:10893-900. 2011
    ..selenium antioxidant activity, Cu(I) coordination of the selenoamino acids methyl-Se-cysteine (MeSeCys) and selenomethionine (SeMet) was investigated...
  35. ncbi Metabolism of selenomethionine by rainbow trout (Oncorhynchus mykiss) embryos can generate oxidative stress
    Vince P Palace
    Department of Fisheries and Oceans, Habitat Impacts Research, Freshwater Institute, 501 University Crescent, Winnipeg, MB, Canada R3T 2N6
    Ecotoxicol Environ Saf 58:17-21. 2004
    ..L-Selenomethionine, the predominant form of selenium in the eggs of oviparous vertebrates, does not generate oxidative radicals ..
  36. doi Uptake, distribution, and speciation of selenoamino acids by human cancer cells: X-ray absorption and fluorescence methods
    Claire M Weekley
    School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia
    Biochemistry 50:1641-50. 2011
    ..The uptake, speciation, and distribution of the common selenoamino acid supplements, selenomethionine (SeMet) and Se-methylselenocysteine (MeSeCys), in A549 human lung cancer cells were investigated using X-ray ..
  37. doi Enhancement of methyl methanesulfonate-induced base excision repair in the presence of selenomethionine on p53-dependent pathway
    Hwa Jin Jung
    Department of Pharmacology, Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, Republic of Korea
    J Med Food 12:340-4. 2009
    b>Selenomethionine (SeMet) has been identified as a chemopreventive antioxidant to activate p53-mediated nucleotide excision repair. In this study, we examined whether p53-mediated base excision repair (BER) might be induced by SeMet...
  38. ncbi Crystal structure of the rat liver fructose-2,6-bisphosphatase based on selenomethionine multiwavelength anomalous dispersion phases
    Y H Lee
    Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA
    Biochemistry 35:6010-9. 1996
    ..5 A resolution. The selenomethionine-substituted protein was induced in the methionine auxotroph, Escherichia coli DL41DE3, purified, and ..
  39. ncbi Selenomethionine incorporation into a protein by cell-free synthesis
    Takanori Kigawa
    RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama, 230 0045 Japan
    J Struct Funct Genomics 2:29-35. 2002
    ..b>Selenomethionine substituted proteins are commonly used for this purpose...
  40. ncbi Determination of methionine and selenomethionine in selenium-enriched yeast by species-specific isotope dilution with liquid chromatography-mass spectrometry and inductively coupled plasma mass spectrometry detection
    Shona McSheehy
    Institute for National Measurement Standards, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R9
    Anal Chem 77:344-9. 2005
    b>Selenomethionine (SeMet) and methionine (Met), liberated by acid hydrolysis of selenium-enriched yeast, were quantified by liquid chromatography-mass spectrometry (LC/MS) using standard additions calibrations as well as isotope dilution (..
  41. ncbi Selenium metabolites in urine of cancer patients receiving L-selenomethionine at high doses
    Doris Kuehnelt
    Institute of Chemistry Analytical Chemistry, Karl Franzens University Graz, Universitaetsplatz 1, 8010 Graz, Austria
    Toxicol Appl Pharmacol 220:211-5. 2007
    ..HPLC/mass spectrometry, the selenium metabolites in urine from five cancer patients receiving high doses of L-selenomethionine over an extended period (2 x 4000 microg Se/day for 7 days, then 4000 microg Se/day for 21 days) as an ..
  42. ncbi Bioavailability of selenium from raw or cured selenomethionine-enriched fillets of Atlantic salmon (Salmo salar) assessed in selenium-deficient rats
    Robin Ornsrud
    Directorate of Fisheries, Institute of Nutrition, Bergen, Norway
    Br J Nutr 87:13-20. 2002
    The bioavailability of Se from raw and cured selenomethionine-enriched (Se-enriched) salmon fillets was assessed in Se-deficient male albino rats (Mol: Wist)...
  43. doi The organic selenium compound selenomethionine modulates bleomycin-induced DNA damage and repair in human leukocytes
    Blanca Laffon
    Toxicology Unit, Department of Psychobiology, University of A Coruna, Edificio de Servicios Centrales de Investigación, Campus Elviña s n, 15071, A Coruna, Spain
    Biol Trace Elem Res 133:12-9. 2010
    The objective of this work was to evaluate the effects of selenomethionine (SeMet) on the induction, repair, and persistence of DNA damage in human leukocytes challenged with bleomycin (BLM)...
  44. pmc Selenomethionine and alpha-tocopherol do not inhibit prostate carcinogenesis in the testosterone plus estradiol-treated NBL rat model
    Nur Ozten
    Department of Pathology, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA
    Cancer Prev Res (Phila) 3:371-80. 2010
    ..In this study, we examined the potential of selenomethionine and alpha-tocopherol to modulate prostate cancer development in the testosterone plus estradiol-treated NBL ..
  45. ncbi Effects of 12 months treatment with L-selenomethionine on serum anti-TPO Levels in Patients with Hashimoto's thyroiditis
    Elias E Mazokopakis
    Department of Internal Medicine, University Hospital of Heraklion, Crete, Greece
    Thyroid 17:609-12. 2007
    ..We studied the effects of selenium (Se) treatment on serum anti-thyroid peroxidase (TPO) levels in Greek patients with Hashimoto's thyroiditis (HT)...
  46. pmc Crystal structure of Bfr A from Mycobacterium tuberculosis: incorporation of selenomethionine results in cleavage and demetallation of haem
    Vibha Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi, India
    PLoS ONE 4:e8028. 2009
    ..In addition, this study provides evidence against the general belief that a selenium derivative of a protein represents its true physiological native structure...
  47. ncbi Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination
    Lucy Stols
    Environmental Research Division, Argonne National Laboratory, Argonne, IL 60439, USA
    J Struct Funct Genomics 5:95-102. 2004
    ..that the approach is also well suited for production of proteins in defined media with incorporation of selenomethionine to facilitate structure determination by multiwavelength anomalous diffraction...
  48. ncbi The nutritional significance, metabolism and toxicology of selenomethionine
    Gerhard Norbert Schrauzer
    Biological Trace Element Research Institute, 2400 Boswell Rd, Suite 200, Chula Vista, CA 91914, USA
    Adv Food Nutr Res 47:73-112. 2003
    ..The expectation is that lower additions to feedstock of equivalent levels of SeMet will suffice to achieve adequacy than currently approved maximum levels of Se in the form of inorganic Se salts...
  49. ncbi Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells
    Norio Yamamoto
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Cancer Gene Ther 10:445-50. 2003
    We have previously shown that the toxic pro-oxidant methylselenol is released from selenomethionine (SeMET) by cancer cells transformed with the adenoviral methionine alpha,gamma-lyase (methioninase, MET) gene cloned from Pseudomonas ..
  50. pmc Apoptosis induced by selenomethionine and methioninase is superoxide mediated and p53 dependent in human prostate cancer cells
    Rui Zhao
    Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4 868, Clinical Science Center, Box 8550, 600 Highland Avenue, Madison, WI 53792, USA
    Mol Cancer Ther 5:3275-84. 2006
    b>Selenomethionine (SeMet) is the chemical form or major component of selenium used for cancer chemoprevention in several clinical trials...
  51. ncbi The in vitro effects of selenomethionine on methylmercury-induced neurotoxicity
    Parvinder Kaur
    Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Olav Kyrresgt 3, N 7489 Trondheim, Norway
    Toxicol In Vitro 23:378-85. 2009
    ..Therefore, we investigated whether 24h. preincubation or 50min. coincubation with selenomethionine (SeMet) was effective in reducing methylmercury (MeHg)-induced cytotoxicity in C6-glioma and B35-neuronal ..
  52. ncbi Profiling of selenomethionine responsive genes in colon cancer by microarray analysis
    Anne Christine Goulet
    Department of Pathology, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA
    Cancer Biol Ther 6:494-503. 2007
    ..Previous studies by our group demonstrated that selenomethionine-induced growth arrest appears to be mediated by activation of ERK and subsequent phosphorylation of RSK and ..
  53. ncbi Selenium and selenomethionine levels in prostate cancer patients
    David W Nyman
    Department of Pharmacology Toxicology, University of Arizona Health Sciences Center, Tucson, AZ 85721 0207, USA
    Cancer Detect Prev 28:8-16. 2004
    Selenium (Se) and selenomethionine (Se-Met) have been identified as potential chemopreventive agents of prostate cancer...
  54. ncbi Oxidation of selenomethionine: some MADness in the method!
    A J Sharff
    Department of Biochemistry, University of Cambridge, England
    Acta Crystallogr D Biol Crystallogr 56:785-8. 2000
    ..Here, it is demonstrated that the anomalous signal from selenomethione (SeMet) substituted proteins can be significantly enhanced by oxidation...
  55. ncbi Biomarkers of exposure and effect as indicators of the interference of selenomethionine on methylmercury toxicity
    Ana P M dos Santos
    Centro de Estudos de Ciências Farmacêuticas, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Toxicol Lett 169:121-8. 2007
    The present study was conducted to clarify the interference of selenomethionine (SeMet) on methylmercury (MeHg) toxicity through the evaluation of changes in biomarkers of exposure and effect in rats exposed to MeHg and co-exposed to MeHg ..
  56. ncbi A phase I and pharmacokinetic study of fixed-dose selenomethionine and irinotecan in solid tumors
    Marwan G Fakih
    Department of Medicine, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Clin Cancer Res 12:1237-44. 2006
    We conducted a phase I study to determine the maximum tolerated dose (MTD) of irinotecan with fixed, nontoxic high dose of selenomethionine.
  57. doi Selenomethionine stimulates expression of glutathione peroxidase 1 and 3 and growth of bovine mammary epithelial cells in primary culture
    S G Miranda
    Department of Animal Science, University of Zulia, Maracaibo, Venezuela 4005
    J Dairy Sci 92:2670-83. 2009
    ..The effect of selenium as selenomethionine (SeMet) on the growth and viability of BMEC and GPx protein expression and activity were also studied...
  58. pmc Reduction of L-methionine selenoxide to seleno-L-methionine by endogenous thiols, ascorbic acid, or methimazole
    Renee J Krause
    Department of Comparative Biosciences and the Center for Molecular and Environmental Toxicology, University of Wisconsin Madison, Madison, WI 53706, United States
    Biochem Pharmacol 77:134-40. 2009
    ..Furthermore, the novel reduction of MetSeO by methimazole provides clear evidence that methimazole should not be used as an alternative FMO substrate when studying FMO-mediated oxidation of SeMet...
  59. ncbi Selenite or selenomethionine interaction with methylmercury on uptake and toxicity showing a weak selenite protection: studies on cultured K-562 cells
    P Frisk
    Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences, Uppsala University, Sweden
    Biol Trace Elem Res 80:251-68. 2001
    ..Human K-562 cells were pretreated or simultaneously treated with either selenite (5 or 50 microM) or selenomethionine (10 or 50 microM) together with (3.5 or 5 microM) MMC...
  60. ncbi Selenomethionine regulates cyclooxygenase-2 (COX-2) expression through nuclear factor-kappa B (NF-kappaB) in colon cancer cells
    Durga P Cherukuri
    Department of Pathology, Arizona Cancer Center, The University of Arizona College of Medicine, Tucson, Arizona 85724, USA
    Cancer Biol Ther 4:175-80. 2005
    Previously, we showed that selenomethionine (Se-Met) inhibits growth of colon cancer cells via suppressing COX-2 expression at both mRNA and protein level...
  61. ncbi Selenium compounds regulate p53 by common and distinctive mechanisms
    Martin L Smith
    Indiana University School of Medicine, Department of Microbiology and Walther Oncology Center, Indianapolis, IN 46202, USA
    Anticancer Res 24:1401-8. 2004
    ..The main dietary form is selenomethionine, which we showed modulated p53 activity by causing redox regulation of key p53 cysteine residues...
  62. ncbi Randomized, placebo-controlled, esophageal squamous cell cancer chemoprevention trial of selenomethionine and celecoxib
    Paul J Limburg
    Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Gastroenterology 129:863-73. 2005
    ..Squamous dysplasia, the accepted histological precursor for esophageal squamous cell carcinoma, represents a potentially modifiable intermediate end point for chemoprevention trials in high-risk populations...
  63. ncbi Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium
    Hassan K Sreenath
    Department of Biochemistry, Center for Eukaryotic Structural Genomics, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1549, USA
    Protein Expr Purif 40:256-67. 2005
    Protocols have been developed and applied in the high-throughput production of selenomethionine labeled fusion proteins using the conditional Met auxotroph Escherichia coli B834...
  64. ncbi Effects of a six month treatment with selenomethionine in patients with autoimmune thyroiditis
    Leonidas H Duntas
    Endocrine Unit, Evgenidion Hospital, University of Athens Medical School, 20 Papadiamantopoulou Str, 11528 Athens, Greece
    Eur J Endocrinol 148:389-93. 2003
    ..Therefore, we performed a randomised, placebo-controlled prospective study to investigate the effects of Se treatment on patients with autoimmune thyroiditis (AIT)...
  65. ncbi Incorporation of selenomethionine into induced intracytoplasmic membrane proteins of Rhodobacter species
    Philip D Laible
    Biosciences Division, Argonne National Laboratory, 9700 S Cass Avenue, Argonne, IL 60439, USA
    J Struct Funct Genomics 6:95-102. 2005
    ..Development of methods for the in vivo substitution of methionine by selenomethionine (SeMet) has revolutionized the process for determination of structures of soluble proteins in recent years...
  66. ncbi Methioninase and selenomethionine but not Se-methylselenocysteine generate methylselenol and superoxide in an in vitro chemiluminescent assay: implications for the nutritional carcinostatic activity of selenoamino acids
    Julian E Spallholz
    Food and Nutrition, College of Human Sciences, Texas Tech University, Lubbock, TX 79409, USA
    Biochem Pharmacol 67:547-54. 2004
    ..methylselenol-generating chemiluminesence by superoxide (O2*-) is shown to be catalytically produced from L-selenomethionine and D,L-selenoethionine, but not from methionine or L-Se-methylselenocysteine (SeMC)...
  67. ncbi Selenomethionine induces sustained ERK phosphorylation leading to cell-cycle arrest in human colon cancer cells
    Anne Christine Goulet
    Department of Pathology, Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, USA
    Carcinogenesis 26:109-17. 2005
    b>Selenomethionine (SeMet) is being tested alone and in combination with other agents in cancer chemoprevention trials...
  68. ncbi Comparative effects of 2 antioxidants, selenomethionine and epigallocatechin-gallate, on catabolic and anabolic gene expression of articular chondrocytes
    Rina Andriamanalijaona
    Laboratory of Connective Tissue Biochemistry, Faculty of Medicine, 14032 Caen Cedex, France
    J Rheumatol 32:1958-67. 2005
    To determine the effects of selenomethionine (Se-met) and epigallocatechin-gallate (EGCg) on gene expression, activation of mitogen-activating kinases, and DNA binding of nuclear factor-kappaB (NF-kappaB) and apolipoprotein-1 (AP-1) in ..
  69. ncbi Selenomethionine and selenocysteine double labeling strategy for crystallographic phasing
    Marie Paule Strub
    Centre de Biochimie Structurale, UMR CNRS 5048, UMR 554 INSERM, Universite Montpellier I, 34090 Cedex, Montpellier, France
    Structure 11:1359-67. 2003
    A protocol for the quantitative incorporation of both selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli is described...
  70. ncbi Changes in serum proteomic patterns by presurgical alpha-tocopherol and L-selenomethionine supplementation in prostate cancer
    Jeri Kim
    Department of Genitourinary Medical Oncology, University of Massachusetts Medical School, Lazare Research Building, Room 504, 364 Plantation Street, Worcester, MA 01605 2324, USA
    Cancer Epidemiol Biomarkers Prev 14:1697-702. 2005
    Evidence of the chemopreventive effects of the dietary antioxidants alpha-tocopherol (vitamin E) and l-selenomethionine (selenium) comes from secondary analysis of two phase III clinical trials that found treatment with these antioxidants ..
  71. ncbi Selenomethionine labeling of recombinant proteins
    Anna M Larsson
    Department of Cell and Molecular Biology, University of Uppsala, Uppsala, Sweden
    Methods Mol Biol 389:165-74. 2007
    b>Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion...
  72. ncbi Production of selenomethionine-labelled proteins using simplified culture conditions and generally applicable host/vector systems
    S A Guerrero
    , Braunschweig, Germany
    Appl Microbiol Biotechnol 56:718-23. 2001
    The amino acid analogue selenomethionine (SeMet) is shown to be efficiently incorporated into recombinant proteins expressed in Escherichia coli grown in a simple minimal medium without the addition of synthetic amino acids...
  73. pmc Reduction of gene repair by selenomethionine with the use of single-stranded oligonucleotides
    Timothy R Schwartz
    Department of Biological Sciences, University of Delaware, Delaware Biotechnology Institute, 15 Innovation Way Newark, DE 19711, USA
    BMC Mol Biol 8:7. 2007
    ..This process is regulated by the activity of various factors and proteins that either elevate or depress the frequency at which gene repair takes place...
  74. ncbi A Phase I and pharmacokinetic study of selenomethionine in combination with a fixed dose of irinotecan in solid tumors
    Marwan G Fakih
    Department of Medicine, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Cancer Chemother Pharmacol 62:499-508. 2008
    We conducted a phase I study to determine the recommended dose of selenomethionine (SLM) in combination with irinotecan that consistently results in a protective plasma selenium (Se) concentrations > 15 microM after 1 week of SLM ..
  75. pmc XANES measurements of the rate of radiation damage to selenomethionine side chains
    James M Holton
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158 2330, USA
    J Synchrotron Radiat 14:51-72. 2007
    The radiation-induced disordering of selenomethionine (SeMet) side chains represents a significant impediment to protein structure solution...
  76. ncbi Selenomethionine inhibits ultraviolet radiation-induced p53 transactivation
    Nicola J Traynor
    Photobiology Unit, Ninewells Hospital, University of Dundee, Dundee, UK
    Photodermatol Photoimmunol Photomed 22:297-303. 2006
    ....
  77. ncbi Selenium (Se) from high-selenium broccoli is utilized differently than selenite, selenate and selenomethionine, but is more effective in inhibiting colon carcinogenesis
    J W Finley
    United States Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, ND 58202, USA
    Biofactors 14:191-6. 2001
    ..amounts of Se from high-Se processed wheat also significantly reduced aberrant crypts (AC), although pure selenomethionine, (the predominant chemical form of Se in wheat), did not significantly reduce AC...
  78. doi Synthesis of selenocysteine and selenomethionine derivatives from sulfur-containing amino acids
    Michio Iwaoka
    Department of Chemistry, School of Science, Tokai University, Kitakaname, Hiratsuka shi, Kanagawa 259 1292, Japan
    Chem Biodivers 5:359-74. 2008
    ..our recent progresses in the transformation from sulfur-containing amino acids to selenocysteine (SeCys) and selenomethionine (SeMet) derivatives are reviewed along with the surveys of general organic methodologies for the synthesis of ..
  79. ncbi Selenomethionine inhibits growth and suppresses cyclooxygenase-2 (COX-2) protein expression in human colon cancer cell lines
    Antonio Baines
    Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA
    Cancer Biol Ther 1:370-4. 2002
    Currently, selenium (in the form of high selenium containing yeast or selenomethionine) is being evaluated for anticancer effects against both human colon polyp recurrence and human prostate cancer, respectively...
  80. ncbi Comparative toxicosis of sodium selenite and selenomethionine in lambs
    Asheesh K Tiwary
    California Animal Health and Food Safety Laboratory System, University of California, Davis, USA
    J Vet Diagn Invest 18:61-70. 2006
    ..The objective of the study reported here was to compare the acute toxicosis caused by organic selenium (selenomethionine) found in plants with that caused by the supplemental, inorganic form of selenium (sodium selenite)...
  81. ncbi Hydrolysis of proteins with methanesulfonic acid for improved HPLC-ICP-MS determination of seleno-methionine in yeast and nuts
    Katarzyna Wrobel
    Department of Chemistry, University of Cincinnati, Cincinnati, OH, 45221 0172, USA
    Anal Bioanal Chem 375:133-8. 2003
    ..5)/methanol (95:5). The results obtained by standard addition showed 816+/-17 micro g g(-1) and 36.2+/-1.5 micro g g(-1) of selenium in the form of Se-methionine in yeast and nuts, respectively (65% and 75% of total selenium)...
  82. doi The effect of selenium, as selenomethionine, on genome stability and cytotoxicity in human lymphocytes measured using the cytokinesis-block micronucleus cytome assay
    Jing Wu
    Nutrigenomics Laboratory, CSIRO Human Nutrition, SA, Australia
    Mutagenesis 24:225-32. 2009
    ..Therefore, a cautious approach to supplementation with Se-met is required to ensure that optimal genome health is achieved without cytotoxic effects...
  83. pmc An essential role of s-adenosyl-L-methionine:L-methionine s-methyltransferase in selenium volatilization by plants. Methylation of selenomethionine to selenium-methyl-L-selenium- methionine, the precursor of volatile selenium
    Abderrahmane Tagmount
    Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720 3102, USA
    Plant Physiol 130:847-56. 2002
    ..Thus, our results provide in vivo evidence that MMT is the key enzyme catalyzing the methylation of Se-Met to SeMM...
  84. pmc Selenomethionine incorporation in proteins expressed in Lactococcus lactis
    Ronnie P A Berntsson
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Protein Sci 18:1121-7. 2009
    ..Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis...
  85. pmc Effects of selenomethionine supplementation on selenium status and thyroid hormone concentrations in healthy adults
    Gerald F Combs
    Grand Forks Human Nutrition Research Center, US Department of Agriculture, Agricultural Research Service, Grand Forks, ND 55202, USA
    Am J Clin Nutr 89:1808-14. 2009
    ..The effects of long-term selenium supplementation on thyroid hormone concentrations are unknown...
  86. pmc Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing
    Michael G Malkowski
    Center for Pediatric Biomedical Research and Departments of Environmental Medicine and Biochemistry and Biophysics, University of Rochester Medical School, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 104:6678-83. 2007
    ..However, extensive replacement of methionine by selenomethionine for anomalous dispersion phasing has proven intractable in yeast...
  87. ncbi Selenomethionine does not affect PSA secretion independent of its effect on LNCaP cell growth
    Suvarna Bhamre
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Prostate 54:315-21. 2003
    Individuals supplemented with selenium have reduced incidence of prostate cancer. This study determines whether selenomethionine specifically affects the secretion of prostate specific antigen (PSA) in vitro.
  88. pmc Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold
    Michael Rossbach
    Institute of Neurobiochemistry, The Protein Chemistry Group, Witten Herdecke University, Stockumer Strasse 10, 58448 Witten, Germany
    BMC Struct Biol 5:7. 2005
    ..Although annotated as a nucleotide kinase, such an activity could not be confirmed for aaTHEP1 experimentally and the in vivo function of aaTHEP1 is still unknown...
  89. pmc Structure at 1.6 A resolution of the protein from gene locus At3g22680 from Arabidopsis thaliana
    Simon T M Allard
    Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin Madison, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:647-50. 2005
    ..Protein At3g22680 shows no structural homology to any other known proteins and represents a new fold in protein conformation space...
  90. pmc Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri
    Cristiane R Guzzo
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, CEP 05508 000, Sao Paulo, SP, Brazil
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:493-5. 2005
    ..6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P2(1) and crystals diffracted to 1.9 A resolution at a synchrotron source...
  91. pmc Crystallization of the C-terminal globular domain of avian reovirus fibre
    Mark J van Raaij
    Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus Sur, E 15782 Santiago de Compostela, Spain
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:651-4. 2005
    ..Therefore, a search is under way for suitable heavy-atom derivatives and attempts are being made to grow protein crystals containing selenomethionine instead of methionine.
  92. ncbi Crystallization and preliminary crystallographic analysis of BbCRASP-1, a complement regulator-acquiring surface protein of Borrelia burgdorferi
    Frank S Cordes
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England
    Acta Crystallogr D Biol Crystallogr 60:929-32. 2004
    ..Crystals of native and selenomethionine-substituted BbCRASP-1 have been obtained and a native data set to 2...
  93. ncbi Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-B
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:957-61. 2004
    ..The native XenA data set includes reflections between 35 and 1.65 A. Four-wavelength MAD data sets from selenomethionine-enriched XenA and from three different ligand complexes are also reported...
  94. ncbi Expression, purification and preliminary crystallographic studies of a single-point mutant of Mos1 mariner transposase
    Julia M Richardson
    School of Biological Sciences, University of Edinburgh, The King s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland
    Acta Crystallogr D Biol Crystallogr 60:962-4. 2004
    ..5 A resolution and show tetragonal symmetry, with unit-cell parameters a = b = 44.5, c = 205.6 A. Multiple-wavelength anomalous data from a selenomethionyl form of the protein and data from a heavy-atom derivative have been collected...
  95. ncbi Structure of the molybdenum-cofactor biosynthesis protein MoaB of Escherichia coli
    Gerd Bader
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Acta Crystallogr D Biol Crystallogr 60:1068-75. 2004
    ..The close structural homology to MogA and the gephyrin and Cnx1 domains suggests that MoaB may bind a hitherto unidentified pterin compound, possibly an intermediate in molybdopterin biosynthesis...
  96. ncbi Crystallization and preliminary X-ray analysis of alpha-isopropylmalate synthase from Mycobacterium tuberculosis
    Nayden Koon
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    Acta Crystallogr D Biol Crystallogr 60:1167-9. 2004
    ..in Mycobacterium tuberculosis (Rv3710) has been cloned, expressed in Escherichia coli, both in native and selenomethionine-substituted forms, and crystallized...
  97. ncbi The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates beta-1,4- and beta-1,3-1,4-mixed linked glucans at a single binding site
    Ana L Carvalho
    Rede de Química e Technologia Centro de Química Fina e Biotechnologia, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    J Biol Chem 279:34785-93. 2004
    ..We propose, therefore, that CtCBM11 contains a single ligand-binding site that displays affinity for both beta-1,4- and beta-1,3-1,4-mixed linked glucans...
  98. pmc Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase
    Alessia Ruggiero
    Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I 80134 Naples, Italy
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:906-9. 2005
    ..77, b = 120.68, c = 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.
  99. pmc Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus
    Ling Xu
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, England
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:778-80. 2006
    ..Crystals of selenomethionine-labelled SA0961 diffract to beyond 2...
  100. ncbi Expression, purification and preliminary crystallographic analysis of dipeptidyl peptidase IV from Porphyromonas gingivalis
    Dean Rea
    Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, England
    Acta Crystallogr D Biol Crystallogr 60:1871-3. 2004
    ..9, c = 310.0 A, beta = 95.0 degrees. There are ten molecules per asymmetric unit, indicating a solvent content of 50%. Data were also collected from selenomethionine-derived crystals and structure solution by SAD or MAD is in progress.
  101. ncbi Selenium metabolites in human urine after ingestion of selenite, L-selenomethionine, or DL-selenomethionine: a quantitative case study by HPLC/ICPMS
    Doris Kuehnelt
    Institute of Chemistry Analytical Chemistry, Karl Franzens University Graz, Universitaetsplatz 1, 8010, Graz, Austria
    Anal Bioanal Chem 383:235-46. 2005
    ..0 mg) as sodium selenite, L-selenomethionine, or DL-selenomethionine. The three separate experiments were performed in duplicate...

Research Grants75

  1. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2009
    ..This trial is testing the hypothesis that 200 5g of L-selenomethionine given as selenized yeast can decrease the rate of second primary tumors in patients who have undergone ..
  2. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven A Belinsky; Fiscal Year: 2010
    ..This trial is testing the hypothesis that 200 5g of L-selenomethionine given as selenized yeast can decrease the rate of second primary tumors in patients who have undergone ..
  3. The influence of selenium on biomarkers of prostate cancer risk
    KARAM E EL BAYOUMY; Fiscal Year: 2010
    ..a double-blind, randomized, placebo-controlled clinical trial of selenium supplementation in the form of selenomethionine (SM=200 5g/day) and SY (240 5g/day and 350 5g/day the higher dose will deliver 200 5g/day of SM;(the ..
  4. The influence of selenium on biomarkers of prostate cancer risk
    Karam El Bayoumy; Fiscal Year: 2009
    ..a double-blind, randomized, placebo-controlled clinical trial of selenium supplementation in the form of selenomethionine (SM=200 5g/day) and SY (240 5g/day and 350 5g/day the higher dose will deliver 200 5g/day of SM;(the ..
  5. Supplemental Se and Vitamin E and Pulmonary Function
    PATRICIA CASSANO; Fiscal Year: 2007
    ..men testing whether daily supplementation with vitamin E (400mg alpha-tocopherol), selenium (200 micrograms selenomethionine) or both vitamin E and selenium can prevent prostate cancer...
  6. Supplemental Se and Vitamin E and Pulmonary Function
    PATRICIA CASSANO; Fiscal Year: 2003
    ..men testing whether daily supplementation with vitamin E (400mg alpha-tocopherol), selenium (200 micrograms selenomethionine) or both vitamin E and selenium can prevent prostate cancer...
  7. Supplemental Se and Vitamin E and Pulmonary Function
    PATRICIA CASSANO; Fiscal Year: 2004
    ..men testing whether daily supplementation with vitamin E (400mg alpha-tocopherol), selenium (200 micrograms selenomethionine) or both vitamin E and selenium can prevent prostate cancer...
  8. Supplemental Se and Vitamin E and Pulmonary Function
    PATRICIA CASSANO; Fiscal Year: 2006
    ..men testing whether daily supplementation with vitamin E (400mg alpha-tocopherol), selenium (200 micrograms selenomethionine) or both vitamin E and selenium can prevent prostate cancer...
  9. Supplemental Se and Vitamin E and Pulmonary Function
    PATRICIA CASSANO; Fiscal Year: 2005
    ..men testing whether daily supplementation with vitamin E (400mg alpha-tocopherol), selenium (200 micrograms selenomethionine) or both vitamin E and selenium can prevent prostate cancer...
  10. HUMAN SELENIUM NUTRITIONAL REQUIREMENT AND BIOMARKERS
    Raymond Burk; Fiscal Year: 2001
    ..There is a non-specific metabolism, involving the selenium in selenomethionine, and a specific metabolism that recognizes selenium and incorporates it into specific selenoproteins and ..
  11. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2005
    ..of DNA lesions in single MN and to determine if known and potentially new pharmacotherapies (creatine and selenomethionine) for mouse ALS attenuate the formation of DNA lesions...
  12. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2005
    ..of DNA lesions in single MN and to determine if known and potentially new pharmacotherapies (creatine and selenomethionine) for mouse ALS attenuate the formation of DNA lesions...
  13. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2003
    ..of DNA lesions in single MN and to determine if known and potentially new pharmacotherapies (creatine and selenomethionine) for mouse ALS attenuate the formation of DNA lesions...
  14. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2004
    ..of DNA lesions in single MN and to determine if known and potentially new pharmacotherapies (creatine and selenomethionine) for mouse ALS attenuate the formation of DNA lesions...
  15. Mechanisms of Motor Neuron Death
    Lee Martin; Fiscal Year: 2006
    ..of DNA lesions in single MN and to determine if known and potentially new pharmacotherapies (creatine and selenomethionine) for mouse ALS attenuate the formation of DNA lesions...
  16. Chemoprevention of arsenic-induced skin cancer
    Habibul Ahsan; Fiscal Year: 2010
    ..4,444 individuals with arsenic-related skin lesions into 4 treatment arms: vitamin E only (100 IU/day), L-selenomethionine only (200 5g/day), both vitamin E and selenium, and placebo...
  17. MEMBRANE PROTEIN TOPOLOGY--CYTOCHROME C
    Martin Caffrey; Fiscal Year: 1992
    ..XSW measurements to determine i) selenium atom location in cytochrome c modified at three or more sites with selenomethionine substituting for the native methionine and ii) ruthenium atom location in cytochrome c derivatized singly at ..
  18. Differential Gene Expression Induced by Antioxidants
    Jeri Kim; Fiscal Year: 2003
    ..The aims of this study are: 1) to identify genes differentially induced by the antioxidants l-selenomethionine (selenium) and alpha-tocopherol (vitamin E), singly and in combination, in normal and cancerous prostate ..
  19. Differential Gene Expression Induced by Antioxidants
    Jeri Kim; Fiscal Year: 2004
    ..The aims of this study are: 1) to identify genes differentially induced by the antioxidants l-selenomethionine (selenium) and alpha-tocopherol (vitamin E), singly and in combination, in normal and cancerous prostate ..
  20. FMOs: Roles in Metabolism and Toxicity
    ADNAN ELFARRA; Fiscal Year: 2006
    ..With most chemicals, the FMO-mediated reaction is a detoxication reaction. However, with compounds such as selenomethionine, a naturally occurring compound that has antioxidant and anticancer properties, and the cysteine S-conjugates ..
  21. FMOs: Roles in Metabolism and Toxicity
    ADNAN ELFARRA; Fiscal Year: 2005
    ..With most chemicals, the FMO-mediated reaction is a detoxication reaction. However, with compounds such as selenomethionine, a naturally occurring compound that has antioxidant and anticancer properties, and the cysteine S-conjugates ..
  22. FMOs: Roles in Metabolism and Toxicity
    ADNAN ELFARRA; Fiscal Year: 2007
    ..With most chemicals, the FMO-mediated reaction is a detoxication reaction. However, with compounds such as selenomethionine, a naturally occurring compound that has antioxidant and anticancer properties, and the cysteine S-conjugates ..
  23. Prevention of PTEN deletion driven prostate cancer by selenium
    Yibin Deng; Fiscal Year: 2011
    ..Trial (SELECT) in October 2008 ahead of schedule because of the failure to demonstrate an efficacy of selenomethionine (SeMet) for PCa prevention in North American men and a slight but non- statistically significant increase in ..
  24. DETERMINANTS OF CLATHRIN STRUCTURE AND FUNCTION
    Joel Ybe; Fiscal Year: 2007
    ..7-2.3 A at Argonne and at the Advanced Light Source. Selenomethionine crystals have been prepared to solve the phasing problem by MAD analysis, while heavy metal replacement and "..
  25. DETERMINANTS OF CLATHRIN STRUCTURE AND FUNCTION
    Joel Ybe; Fiscal Year: 2005
    ..7-2.3 A at Argonne and at the Advanced Light Source. Selenomethionine crystals have been prepared to solve the phasing problem by MAD analysis, while heavy metal replacement and "..
  26. DETERMINANTS OF CLATHRIN STRUCTURE AND FUNCTION
    Joel Ybe; Fiscal Year: 2003
    ..7-2.3 A at Argonne and at the Advanced Light Source. Selenomethionine crystals have been prepared to solve the phasing problem by MAD analysis, while heavy metal replacement and "..
  27. DETERMINANTS OF CLATHRIN STRUCTURE AND FUNCTION
    Joel Ybe; Fiscal Year: 2004
    ..7-2.3 A at Argonne and at the Advanced Light Source. Selenomethionine crystals have been prepared to solve the phasing problem by MAD analysis, while heavy metal replacement and "..
  28. DETERMINANTS OF CLATHRIN STRUCTURE AND FUNCTION
    Joel Ybe; Fiscal Year: 2006
    ..7-2.3 A at Argonne and at the Advanced Light Source. Selenomethionine crystals have been prepared to solve the phasing problem by MAD analysis, while heavy metal replacement and "..
  29. STRUCTURE AND ASSEMBLY OF THE HIV1 CAPSID PROTEIN
    Christopher Hill; Fiscal Year: 2001
    ..363, and grown crystals of both the native and selenomethionine- substituted proteins. Very recently, we have collected complete data sets at 2...
  30. STRUCTURE AND ASSEMBLY OF THE HIV1 CAPSID PROTEIN
    Christopher Hill; Fiscal Year: 2000
    ..363, and grown crystals of both the native and selenomethionine- substituted proteins. Very recently, we have collected complete data sets at 2...
  31. STRUCTURE AND ASSEMBLY OF THE HIV1 CAPSID PROTEIN
    Christopher Hill; Fiscal Year: 1999
    ..363, and grown crystals of both the native and selenomethionine- substituted proteins. Very recently, we have collected complete data sets at 2...
  32. CYSTEINE CONJUGATE SULFOXIDATION BY MULTIPLE FMOS
    ADNAN ELFARRA; Fiscal Year: 2001
    ..Similar studies will be conducted with methionine and selenomethionine, structural analogues of cysteine S-conjugates which we have also identified as FMO substrates and are known ..
  33. CYSTEINE CONJUGATE SULFOXIDATION BY MULTIPLE FMOS
    ADNAN ELFARRA; Fiscal Year: 2004
    ..Similar studies will be conducted with methionine and selenomethionine, structural analogues of cysteine S-conjugates which we have also identified as FMO substrates and are known ..
  34. CYSTEINE CONJUGATE SULFOXIDATION BY MULTIPLE FMOS
    ADNAN ELFARRA; Fiscal Year: 1999
    ..Similar studies will be conducted with methionine and selenomethionine, structural analogues of cysteine S-conjugates which we have also identified as FMO substrates and are known ..
  35. CYSTEINE CONJUGATE SULFOXIDATION BY MULTIPLE FMOS
    ADNAN ELFARRA; Fiscal Year: 2000
    ..Similar studies will be conducted with methionine and selenomethionine, structural analogues of cysteine S-conjugates which we have also identified as FMO substrates and are known ..
  36. Effects of Organic Selenium Against Colon Cancer
    Mark Nelson; Fiscal Year: 2002
    ..However, the mechanism by which organic selenium exerts its anticancer effects remains to be identified. Selenomethionine is the predominant form of selenium in the yeast...
  37. Colorectal Neoplasia in SELECT Participants
    M PETER LANCE; Fiscal Year: 2010
    ..The SELECT Trial will assess the effect of selenium, as L-selenomethionine, and vitamin E alone and in combination on the clinical incidence of prostate cancer in 35,584 now-randomized ..
  38. Colorectal Neoplasia in SELECT Participants
    M Lance; Fiscal Year: 2009
    ..The SELECT Trial will assess the effect of selenium, as L-selenomethionine, and vitamin E alone and in combination on the clinical incidence of prostate cancer in 35,584 now-randomized ..
  39. Colorectal Neoplasia in SELECT Participants
    M Lance; Fiscal Year: 2007
    ..The SELECT Trial will assess the effect of selenium, as L-selenomethionine, and vitamin E alone and in combination on the clinical incidence of prostate cancer in 35,584 now-randomized ..
  40. SELENIUM CHEMOPREVENTION OF MAMMARY CARCINOGENESIS
    Clement Ip; Fiscal Year: 1991
    ..Aim 1 is designed to study the in vivo metabolism of Se under conditions in which selenite and selenomethionine are found to have anticarcinogenic activities...
  41. A new method for delivery of selinium for prevention of melanoma
    Pamela Cassidy; Fiscal Year: 2006
    ..Aim 2: Evaluate new formulations of Se- methylselenocysteine (SeMSec) and selenomethionine (SeMet) for the delivery of selenium to the skin of mice...
  42. A new method for delivery of selinium for prevention of melanoma
    Pamela Cassidy; Fiscal Year: 2007
    ..Aim 2: Evaluate new formulations of Se- methylselenocysteine (SeMSec) and selenomethionine (SeMet) for the delivery of selenium to the skin of mice...
  43. CHEMISTRY AND BIOLOGY OF HUMAN AND OTHER GONADOTROPINS
    OM BAHL; Fiscal Year: 1992
    ..More importantly, it has been possible to substitute selenomethionine for methionine in hCG...
  44. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2007
    ..Ingestion of L-selenomethionine has been associated with a 50% decrease in expected rates of lung cancer...
  45. SELENIUM TOXICITY: SELENOCYSTEINE IN PROTEINS
    ALEX SHRIFT; Fiscal Year: 1980
    ..organisms, such as the selenium accumulating Astragali, are believed unable to recognize selenocystein and selenomethionine, resulting in the exclusion of these selenoamino acids from proteins...
  46. Genetic Analysis of Selenium Toxicity
    Annette Estevez; Fiscal Year: 2003
    ..In Caenorhabditis elegans exposure to selenomethionine (SeMet) causes progressive paralysis, decreased fertility, and growth retardation of adults derived from ..
  47. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2009
    ..for resected Stage I lung cancer patients participating in a Phase III Chemoprevention trial evaluating L- selenomethionine. The studies proposed in this competitive supplement will complement and extend the work in the parent grant ..
  48. Genetic Analysis of Selenium Toxicity
    Annette Estevez; Fiscal Year: 2004
    ..In Caenorhabditis elegans exposure to selenomethionine (SeMet) causes progressive paralysis, decreased fertility, and growth retardation of adults derived from ..
  49. Genetic Analysis of Selenium Toxicity
    Annette Estevez; Fiscal Year: 2005
    ..In Caenorhabditis elegans exposure to selenomethionine (SeMet) causes progressive paralysis, decreased fertility, and growth retardation of adults derived from ..
  50. Clinical Trial of Selenium and Prostate Biomarkers
    James Marshall; Fiscal Year: 2010
    ..scheduled for brachythrapy and prostatectomy to pretreatment supplementation by daily dose of 400 meg selenomethionine, or to placebo...
  51. Clinical Trial of Selenium and Prostate Biomarkers
    James Marshall; Fiscal Year: 2009
    ..scheduled for brachythrapy and prostatectomy to pretreatment supplementation by daily dose of 400 meg selenomethionine, or to placebo...
  52. Clinical Trial of Selenium and Prostate Biomarkers
    James Marshall; Fiscal Year: 2007
    ..scheduled for brachythrapy and prostatectomy to pretreatment supplementation by daily dose of 400 meg selenomethionine, or to placebo...
  53. Selenium Prevention of Tobacco Smoke-Induced Lung Cancer
    Teresa Fan; Fiscal Year: 2005
    ..1) To examine whether dietary Se supplement in the form of selenized yeast (Se-yeast) or its major component selenomethionine (Se-Met) is chemopreventive for mice that have "quit" smoking; 2) To characterize biomarkers of Se action ..
  54. Selenium Prevention of Tobacco Smoke-Induced Lung Cancer
    Teresa Fan; Fiscal Year: 2004
    ..1) To examine whether dietary Se supplement in the form of selenized yeast (Se-yeast) or its major component selenomethionine (Se-Met) is chemopreventive for mice that have "quit" smoking; 2) To characterize biomarkers of Se action ..
  55. Selenium Prevention of Tobacco Smoke-Induced Lung Cancer
    Teresa Fan; Fiscal Year: 2003
    ..1) To examine whether dietary Se supplement in the form of selenized yeast (Se-yeast) or its major component selenomethionine (Se-Met) is chemopreventive for mice that have "quit" smoking; 2) To characterize biomarkers of Se action ..
  56. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2004
    ..Ingestion of L-selenomethionine has been associated with a 50% decrease in expected rates of lung cancer...
  57. CHEMOPREVENTION OF LUNG CANCER BY ORGANOSELENIUM
    Karam El Bayoumy; Fiscal Year: 2001
    ..4- (methylnitrosamino)1-(3-pyridyl)1-butanone (NNK), in female A/J mice while the naturally occurring selenomethionine (SM) found in selenium- enriched had no female A/J mice while the naturally occurring selenomethionine (SM) ..
  58. Methyl-selenium for prostate cancer chemoprevention
    Junxuan Lu; Fiscal Year: 2009
    The ongoing Selenium-vitamin E Cancer Trial (SELECT) is testing the efficacy of selenium (Se) in the form of selenomethionine (SeMet) alone or in combination with vitamin E to prevent prostate cancer (PCa) in a cohort of some 32,400 ..
  59. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2006
    ..Ingestion of L-selenomethionine has been associated with a 50% decrease in expected rates of lung cancer...
  60. Methyl-selenium for prostate cancer chemoprevention
    Junxuan Lu; Fiscal Year: 2007
    The ongoing Selenium-vitamin E Cancer Trial (SELECT) is testing the efficacy of selenium (Se) in the form of selenomethionine (SeMet) alone or in combination with vitamin E to prevent prostate cancer (PCa) in a cohort of some 32,400 ..
  61. X RAY STRUCTURE OF UDP GALACTOPYRANOSE MUTASE
    Michael McNeil; Fiscal Year: 2000
    ..0, beta = 96.38_). Dr. Naismith's group has identified a platinum derivative of the E. coli enzyme using heavy metal soaking experiments and crystallized selenomethionine E. coli UDP-galactopyranose mutase.
  62. Methyl-selenium for prostate cancer chemoprevention
    Junxuan Lu; Fiscal Year: 2010
    The ongoing Selenium-vitamin E Cancer Trial (SELECT) is testing the efficacy of selenium (Se) in the form of selenomethionine (SeMet) alone or in combination with vitamin E to prevent prostate cancer (PCa) in a cohort of some 32,400 ..
  63. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2006
    ..Ingestion of L-selenomethionine has been associated with a 50% decrease in expected rates of lung cancer...
  64. X RAY STRUCTURE OF UDP GALACTOPYRANOSE MUTASE
    Michael McNeil; Fiscal Year: 1999
    ..0, beta = 96.38_). Dr. Naismith's group has identified a platinum derivative of the E. coli enzyme using heavy metal soaking experiments and crystallized selenomethionine E. coli UDP-galactopyranose mutase.
  65. STRUCTURAL BASIS OF GANGLIOSIDE RECOGNITION
    Christine Wright; Fiscal Year: 2001
    ..The crystal structure of the selenomethionine mutant of the GM2-AP has been solved at 2.4 A by multiwavelength anomalous diffraction...
  66. STRUCTURAL BASIS OF GANGLIOSIDE RECOGNITION
    Christine Wright; Fiscal Year: 2002
    ..The crystal structure of the selenomethionine mutant of the GM2-AP has been solved at 2.4 A by multiwavelength anomalous diffraction...
  67. STRUCTURAL BASIS OF GANGLIOSIDE RECOGNITION
    Christine Wright; Fiscal Year: 2003
    ..The crystal structure of the selenomethionine mutant of the GM2-AP has been solved at 2.4 A by multiwavelength anomalous diffraction...
  68. STRUCTURAL BASIS OF GANGLIOSIDE RECOGNITION
    Christine Wright; Fiscal Year: 2000
    ..The crystal structure of the selenomethionine mutant of the GM2-AP has been solved at 2.4 A by multiwavelength anomalous diffraction...
  69. SELENIUM CHEMOPREVENTION OF MAMMARY CARCINOGENESIS
    Clement Ip; Fiscal Year: 1990
    ..experiments in the quantitative analysis of the proposed synergisms between selenium (both selenite and selenomethionine) and vitamin E in protection against mammary tumorigenesis...
  70. Biomarkers to Assess Selenium Chemoprevention for NSCLC
    Steven Belinsky; Fiscal Year: 2003
    ..Ingestion of L-selenomethionine has been associated with a 50% decrease in expected rates of lung cancer...
  71. Development of selenomethionine resistant yeast
    TERRI KINZY; Fiscal Year: 2007
    ..In this aspect, one of the most important techniques now routinely used is the production of selenomethionine (SeMet) substituted recombinant proteins...