Genomes and Genes
Summary: Phosphate salt of carbamic acid.
Publications227 found, 100 shown here
- Mutational analysis of carbamyl phosphate synthetase. Substitution of Glu841 leads to loss of functional coupling between the two catalytic domains of the synthetase subunitF Guillou
Department of Molecular Genetics, Public Health Research Institute, New York, New York 10016
Biochemistry 31:1656-64. 1992The synthetase subunit of Escherichia coli carbamyl phosphate synthetase has two catalytic nucleotide-binding domains, one involved in the activation of HCO3- and the second in phosphorylation of carbamate...
- Catalytic domains of carbamyl phosphate synthetase. Glutamine-hydrolyzing site of Escherichia coli carbamyl phosphate synthetaseS D Rubino
J Biol Chem 261:11320-7. 1986We present evidence that cysteine 269 of the small subunit of Escherichia coli carbamyl phosphate synthetase is essential for the hydrolysis of glutamine...
- Insights into the mechanism and regulation of pyruvate carboxylase by characterisation of a biotin-deficient mutant of the Bacillus thermodenitrificans enzymeAbdussalam Adina-Zada
School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Int J Biochem Cell Biol 40:1743-52. 2008..Attempts to trap the putative carboxyphosphate intermediate in K1112A using diazomethane were unsuccessful...
- The structure of carbamoyl phosphate synthetase determined to 2.1 A resolutionJ B Thoden
Institute for Enzyme Research, The Graduate School, and Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53705, USA
Acta Crystallogr D Biol Crystallogr 55:8-24. 1999..The carboxyphosphate and carbamoyl phosphate synthetic components, while topologically equivalent, are structurally different, as would be expected in light of their separate biochemical functions...
- Carbamate kinase: New structural machinery for making carbamoyl phosphate, the common precursor of pyrimidines and arginineA Marina
Instituto de Biomedicina de Valencia CSIC, Spain
Protein Sci 8:934-40. 1999..The orientation of the crevices in the dimer precludes their physical cooperation in the catalytic process. Such cooperation is not needed in the CK reaction but is a requirement of the mechanism of CPSs...
- Tertiary and quaternary conformational changes in aspartate transcarbamylase: a normal mode studyA Thomas
Laboratoire de Dynamique Moleculaire, Institut de Biologie Structurale Jean Pierre Ebel, Grenoble, France
Proteins 34:96-112. 1999..The particular dynamic behavior of these residues is examined in the light of biochemical results to obtain insights into their role in the transmission of the allosteric signal...
- Half of Saccharomyces cerevisiae carbamoyl phosphate synthetase produces and channels carbamoyl phosphate to the fused aspartate transcarbamoylase domainV Serre
Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, UMR 7631 CNRS Université Pierre et Marie Curie, 96 Bd Raspail 75006 Paris, France
J Biol Chem 274:23794-801. 1999....
- Deconstruction of the catalytic array within the amidotransferase subunit of carbamoyl phosphate synthetaseX Huang
Department of Chemistry, Texas A and M University, College Station, Texas, 77843, USA
Biochemistry 38:15909-14. 1999..It is concluded that contrary to the original proposal for the catalytic triad, Glu-355 is not an essential residue for catalysis. The results are consistent with Ser-47 and Gln-273 playing significant roles in the binding of glutamine...
- The 1.5 A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight inS Ramon-Maiques
Consejo Superior de Investigaciones Cientificas IBV CSIC, Instituto de Biomedicina de Valencia, C Jaime Roig 11, Valencia, 46010, Spain
J Mol Biol 299:463-76. 2000..The structure provides the first information on substrate binding and catalysis in CKs, and suggests that the slow rate at 37 degrees C is possibly a consequence of slow product dissociation...
- Regulatory changes in the control of carbamoyl phosphate synthetase induced by truncation and mutagenesis of the allosteric binding domainR M Czerwinski
Department of Chemistry, Texas A and M University, College Station 77843, USA
Biochemistry 34:13920-7. 1995..Six conserved amino acids within the allosteric domain were mutated.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Direct observation of an altered quaternary-structure transition in a mutant aspartate transcarbamoylaseH Tsuruta
Stanford Synchrotron Radiation Laboratory, California, USA
Proteins 31:383-90. 1998..They also illustrate the crucial role played by domain motions and quaternary-structure changes for both the homotropic and heterotropic aspects of allostery...
- Ligation alters the pathway of urea-induced denaturation of the catalytic trimer of Escherichia coli aspartate transcarbamylaseS Bromberg
Department of Biochemistry, University of Minnesota, St Paul 55108
Protein Sci 3:1236-44. 1994..c3) of aspartate transcarbamylase from Escherichia coli at low temperature in the absence and presence of carbamyl phosphate (CP; a substrate), N-(phosphonacetyl)-L-Asp (PALA; a bisubstrate analog), and 2 anionic inhibitors, Cl- and ..
- Carbamoylphosphate serves as the source of CN(-), but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutrophaOliver Lenz
Humboldt Universitat zu Berlin, Institut für Biologie Mikrobiologie, Chausseestrasse 117, Berlin, Germany
FEBS Lett 581:3322-6. 2007..Incorporation of label from (13)CO exclusively into the carbonyl ligand indicates that free CO is a possible precursor in carbonyl ligand biosynthesis...
- Structural model of the R state of Escherichia coli aspartate transcarbamoylase with substrates boundJie Wang
Department of Chemistry, Boston College, Merkert Chemistry Center, Chestnut Hill, MA 02467, USA
J Mol Biol 371:1261-73. 2007..By combining these data with previously determined structures we can now visualize the allosteric transition through detailed atomic motions that underlie the molecular mechanism...
- Importance of ornithine transcarbamylase (OTC) deficiency in small intestine for urinary orotic acid excretion: analysis of OTC-deficient spf-ash mice with OTC transgeneT Saheki
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan
Biochim Biophys Acta 1270:87-93. 1995..These results suggest that the level of OTC activity in the small intestine is important for production of orotic acid...
- Acetylornithine transcarbamylase: a novel enzyme in arginine biosynthesisHiroki Morizono
Children s Research Institute, Children s National Medical Center, 111 Michigan Avenue NW, Washington, DC 20010, USA
J Bacteriol 188:2974-82. 2006..coli and show that it catalyzes the formation of N-acetyl-L-citrulline from N-acetyl-L-ornithine and carbamyl phosphate. We now designate this enzyme as an acetylornithine transcarbamylase...
- Apparent cooperativity for carbamoylphosphate in Escherichia coli aspartate transcarbamoylase only reflects cooperativity for aspartateP England
Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, URA CNRS 1682, Universite Pierre et Marie Curie, Paris, France
Eur J Biochem 222:775-80. 1994....
- Kinetics of the quaternary structure change of aspartate transcarbamylase triggered by succinate, a competitive inhibitorH Tsuruta
Stanford Synchrotron Radiation Laboratory, SLAC, California 94309 0210
Biochemistry 33:10007-12. 1994..This is consistent with a concerted structural transition of ATCase.(ABSTRACT TRUNCATED AT 250 WORDS)..
- A 70-amino acid zinc-binding polypeptide fragment from the regulatory chain of aspartate transcarbamoylase causes marked changes in the kinetic mechanism of the catalytic trimerB B Zhou
Department of Molecular and Cell Biology, University of California, Berkeley 94720
Protein Sci 3:967-74. 1994..The rate of dissociation of carbamoyl phosphate from the Zn domain-C trimer complex was about 10(-2) that from C trimer.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Weakening of the interface between adjacent catalytic chains promotes domain closure in Escherichia coli aspartate transcarbamoylaseD P Baker
Merkert Chemistry Center, Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02167 3860, USA
Protein Sci 4:258-67. 1995..Solution X-ray scattering was also used to investigate the structural consequences of nucleotide binding to the Glu-50/Asp-100-->Ala enzyme...
- HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe] hydrogenase maturationAthanasios Paschos
Mikrobiologie, Department I der Fakultät für Biologie, Ludwig Maximilians Universitat Munchen, Maria Ward Strasse 1a, D 80638 Munich, Germany
J Biol Chem 277:49945-51. 2002..The results are discussed in terms of the involvement of HypF in the conversion of carbamoyl phosphate to the CN ligand...
- Three-dimensional structure of carbamoyl phosphate and succinate bound to aspartate carbamoyltransferaseJ E Gouaux
Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138
Proc Natl Acad Sci U S A 85:4205-8. 1988..In comparing the structure of the active sites in the PALA-enzyme complex to the active sites in the carbamoyl phosphate-succinate-enzyme complex, we find that they are similar...
- Perforation of the tunnel wall in carbamoyl phosphate synthetase derails the passage of ammonia between sequential active sitesJungwook Kim
Department of Chemistry, Texas A and M University, P O Box 30012, College Station, Texas 77842 3012, USA
Biochemistry 43:5334-40. 2004..Overall, the catalytic constants of the mutant were consistent with the model where the channeling of ammonia has been disrupted due to the leakage from the ammonia tunnel to the protein exterior...
- Structural defects within the carbamate tunnel of carbamoyl phosphate synthetaseJungwook Kim
Department of Chemistry, Texas A and M University, P O Box 30012, College Station, Texas 77843 3012, USA
Biochemistry 41:12575-81. 2002..The partial reactions of CPS have not been significantly impaired by these mutations, and thus, the catalytic machinery at the individual active sites has not been functionally perturbed...
- Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycleMelanie Blokesch
Microbiology, Department I Biology, University of Munich, Maria Ward Strasse 1a, D 80638 Munich, Germany
J Mol Biol 324:287-96. 2002..On the basis of the results, a cycle of HypC activity is proposed whose function is to transfer the iron atom that has been liganded at the HypC-HypD complex to the precursor of the large hydrogenase subunit...
- Taming of a poison: biosynthesis of the NiFe-hydrogenase cyanide ligandsStefanie Reissmann
Department Biologie I, Mikrobiologie, University of Munich, Maria Ward Strasse 1a, D 80638 Munich, Germany
Science 299:1067-70. 2003..This finding underscores a striking parallel between biochemistry and organometallic chemistry in the formation of an iron-cyano complex...
- Metabolic channelling of carbamoyl phosphate in the hyperthermophilic archaeon Pyrococcus furiosus: dynamic enzyme-enzyme interactions involved in the formation of the channelling complexJ Massant
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussels, Belgium
Biochem Soc Trans 32:306-9. 2004....
- Extent of genetic lesions of the arginine and pyrimidine biosynthetic pathways in Lactobacillus plantarum, L. paraplantarum, L. pentosus, and L. casei: prevalence of CO(2)-dependent auxotrophs and characterization of deficient arg genes in L. plantarumFrançoise Bringel
Laboratoire de Dynamique, Expression et Evolution des génomes de micro organismes, FRE 2326 Université Louis Pasteur CNRS, 28 rue Goethe, 67083 Strasbourg, France
Appl Environ Microbiol 69:2674-83. 2003..These HCR auxotrophs may have had their CP cellular pool-related genes altered or deregulated...
- Kinetic characterization of yeast pyruvate carboxylase isozyme Pyc1 and the Pyc1 mutant, C249AJoy P Branson
Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Biochemistry 43:1075-81. 2004....
- Isolation and partial characterization of an argR mutant of Salmonella typhimuriumR A Kelln
J Bacteriol 128:528-35. 1976..Ingraham, Abstr. Annu. Meet. Am. Soc. Microbiol. 1975, K169, p. 175) have shown constitutive synthesis of carbamyl phosphate synthetase in the argR mutant, the regulation of the synthesis of the last five enzymes of the pyrimidine ..
- A functional analysis of the allosteric nucleotide monophosphate binding site of carbamoyl phosphate synthetaseOlivier A Pierrat
Department of Chemistry, Texas A and M University, College Station, Texas, 77842 3012
Arch Biochem Biophys 400:34-42. 2002..With the exception of V994A, these mutations had a modest influence on the binding and subsequent allosteric effects by UMP and IMP...
- Molecular physiology of phosphoryl group transfer from carbamoyl phosphate by a hyperthermophilic enzyme at low temperatureSantiago Ramon-Maiques
Instituto de Biomedicina de Valencia CSIC, Jaume Roig 11, 46010 Valencia, Spain
Biochemistry 41:3916-24. 2002..Probably the catalysis of the phosphoryl group transfer is less efficient at low temperature, as suggested by structural data showing that Lys131 is improperly positioned to assist the transfer...
- Metabolic channeling of carbamoyl phosphate, a thermolabile intermediate: evidence for physical interaction between carbamate kinase-like carbamoyl-phosphate synthetase and ornithine carbamoyltransferase from the hyperthermophile Pyrococcus furiosusJan Massant
Department of Microbiology, Vrije Universiteit Brussel, Flanders Interuniversity Institute for Biotechnology, Universite Libre de Bruxelles, Belgium
J Biol Chem 277:18517-22. 2002..Therefore, by physically interacting with each other, CKase and OTCase prevent the thermodenaturation of carbamoyl phosphate in the aqueous cytoplasmic environment...
- Site-directed mutagenesis of Arg60 and Cys271 in ornithine transcarbamylase from rat liverS McDowall
Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia
Protein Eng 4:73-7. 1990..The small loss in binding energy and relatively high residual catalytic activity of the mutant strongly suggests that a number of other residues are involved in the binding of ornithine.(ABSTRACT TRUNCATED AT 250 WORDS)..
- cDNA cloning of two isoforms of ornithine carbamoyltransferase from Canavalia lineata leaves and the effect of site-directed mutagenesis of the carbamoyl phosphate binding siteY Lee
School of Biological Sciences, Seoul National University, Korea
Plant Mol Biol 46:651-60. 2001..It is assumed that the substrate specificity of ClOCT1 or ClOCT2 proteins partially depends on the amino acid sequence of the CP-binding site...
- In Pseudomonas syringae pv. phaseolicola, expression of the argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase, is regulated indirectly by temperature and directly by a precursor resembling carbamoylphosphateKarina López-López
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados CINVESTAV del Instituto Politecnico Nacional IPN, Unidad Irapuato, Irapuato, Guanajuato, C P 36500, Mexico
J Bacteriol 186:146-53. 2004..These results indicate that argK expression is independent of temperature and is regulated directly by a compound resembling the inorganic moiety of phaseolotoxin...
- New experimental approaches for investigating interactions between Pyrococcus furiosus carbamate kinase and carbamoyltransferases, enzymes involved in the channeling of thermolabile carbamoyl phosphateJan Massant
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Pleinlaan 2, B 1050 Brussel, Belgium
Archaea 1:365-73. 2005....
- Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal centerJ B Thoden
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
Biochemistry 40:6989-97. 2001..The ensuing tetrahedral intermediate collapses with C-O bond cleavage and expulsion of the hydroxide which then bridges the binuclear metal center...
- Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi. Insights into cooperative and allosteric mechanismsSigrid Van Boxstael
Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium
FEBS J 272:2670-83. 2005..In contrast to the same enzyme in P. abyssi cellular extracts, the pure enzyme is inhibited by the carbamyl phosphate analogue phosphonacetate; this difference supports the idea that in native cells ATCase interacts with ..
- The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesisV Durbecq
Laboratoire de Microbiologie, Universite Libre de Bruxelles, Brussels, Belgium
Proc Natl Acad Sci U S A 94:12803-8. 1997..The gene coding for this CK-like CPS was called cpkA...
- Thermodynamics of the reactions of carbamoyl phosphateRobert A Alberty
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Arch Biochem Biophys 451:17-22. 2006..As usual the increase in the number of enzyme-catalyzed reactions for which apparent equilibrium constants can be calculated is larger than the number of reactions required to obtain the thermodynamic properties of the species involved...
- Low carbamoyl phosphate pools may drive Lactobacillus plantarum CO2-dependent growth phenotypeFrançoise Bringel
UMR 7156 Université Louis Pasteur, CNRS Génétique Moléculaire, Genomique, Microbiologie, Département Microorganismes, Génomes, Environnement, Strasbourg, France
J Mol Microbiol Biotechnol 14:22-30. 2008..plantarum isolates...
- Protein expression under uracil privation in Lactobacillus sakeiA Marceau
, INRA, Domaine de Vilvert, 78350 Jouy en Josas, France
FEMS Microbiol Lett 200:49-52. 2001..Two of these polypeptides were identified as orotate phosphoribosyl transferase, catalyzing the fifth step of pyrimidine biosynthesis, and PyrR, the transcriptional regulator of the pyr operon, respectively...
- An engineered blockage within the ammonia tunnel of carbamoyl phosphate synthetase prevents the use of glutamine as a substrate but not ammoniaX Huang
Department of Chemistry, Texas A and M University, College Station, Texas 77843, USA
Biochemistry 39:3240-7. 2000..These results suggest the existence of an alternate route to the bicarbonate phosphorylation site when ammonia is provided as an external nitrogen source...
- Glutamine-dependent carbamoyl-phosphate synthetase and other enzyme activities related to the pyrimidine pathway in spleen of Squalus acanthias (spiny dogfish)P M Anderson
Department of Biochemistry, University of Minnesota, Duluth 55812
Biochem J 261:523-9. 1989..Biol. Chem. 262, 198-202] that dogfish express two different glutamine synthetases, and (3) provide indirect evidence that glutamine may not be available in liver for biosynthetic reactions other than urea formation...
- Urea cycle of Fasciola gigantica: purification and characterization of arginaseSaleh A Mohamed
Molecular Biology Department, National Research Centre, Cairo, Egypt
Comp Biochem Physiol B Biochem Mol Biol 142:308-16. 2005..The enzyme was activated with Mn2+, where the other metals Fe2+, Ca2+, Hg2+, Ni2+, Co2+ and Mg2+ had inhibitory effects...
- African sharptooth catfish Clarias gariepinus does not detoxify ammonia to urea or amino acids but actively excretes ammonia during exposure to environmental ammoniaYuen K Ip
Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Road, Singapore 117543, Republic of Singapore
Physiol Biochem Zool 77:242-54. 2004..These results represent the first report on a freshwater fish (C. gariepinus) adopting active excretion of ammonia (probably NH4+) as a major strategy to defend against ammonia toxicity when exposed to environmental ammonia...
- Cloning of cDNA coding for carbamyl phosphate synthetase I and changes in levels of CPS1 mRNA during hepatocarcinogenesisS J Wu
Faculty of Basic Medical Sciences, Peking Union Medical College, Beijing
Sci Sin B 31:197-203. 1988cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insert complementary to the mRNA for CPS1 followed by hybrid-selected translation screening...
- Y-box binding protein-1 down-regulates expression of carbamoyl phosphate synthetase-I by suppressing CCAAT enhancer-binding protein-alpha function in miceYen Rong Chen
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
Gastroenterology 137:330-40. 2009..Despite expression of C/EBPalpha, CPS1 is not expressed in fetal liver, indicating an additional factor is involved in the regulation of CPS1 expression. The aim of this study was to elucidate the mechanism of CPS1 expression...
- On the origin of biochemistry at an alkaline hydrothermal ventWilliam Martin
Institute of Botany, University of Dusseldorf, 40225 Dusseldorf, Germany
Philos Trans R Soc Lond B Biol Sci 362:1887-925. 2007....
- Effects of short-chain acyl-CoA dehydrogenase deficiency on development expression of metabolic enzyme genes in the mouseM E Hinsdale
Department of Comparative Medicine, School of Medicine, University of Alabama at Birmingham 35294, USA
Biochem Mol Med 57:106-15. 1996..SCAD) acyl-CoA dehydrogenases, pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), carbamyl phosphate synthetase I (CPS), ornithine transcarbamylase (OTC), and argininosuccinate synthetase (AS) in fed or fasted ..
- N2-hydroxyguanosine 5'-monophosphate is a time-dependent inhibitor of Escherichia coli guanosine monophosphate synthetaseM L Deras
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
Biochemistry 38:303-10. 1999..In addition, these results complement recent structural studies of GMPS and implicate the formation of the XMP-adenylate intermediate inducing a probable conformational change that stimulates the hydrolysis of glutamine...
- Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCRHeleen Nailis
Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B 9000, Ghent, Belgium
BMC Mol Biol 7:25. 2006..To validate our normalization strategies we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts...
- Uridine and cytidine nucleotide synthesis in renal hypertrophy: biochemical differences in response to the growth stimulus of diabetes and unilateral nephrectomyS Kunjara
Department of Biochemistry, University College and Middlesex School of Medicine, London, Great Britain
Biochem Med Metab Biol 47:168-80. 1992....
- Molecular biology and biochemistry of malarial parasite pyrimidine biosynthetic pathwayJerapan Krungkrai
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Southeast Asian J Trop Med Public Health 34:32-43. 2003..The genes encoding these enzymes were identified in order, from the first to the sixth step, as CPSII (carbamyl phosphate synthase II), ATC (aspartate transcarbamylase), DHO (dihydroorotase), DHOD (dihydroorotate dehydrogenase, ..
- Ornithine transcarbamylase deficiency: a urea cycle defectNeil Gordon
Eur J Paediatr Neurol 7:115-21. 2003..Liver transplant can be considered when symptoms are life-threatening, although there may be severe complications.Gene replacement therapy is the hope of the future...
- Orotic acid excretion and arginine metabolismMargaret E Brosnan
Department of Biochemistry, Memorial University of Newfoundland, St John s, NL, Canada A1B 3X9
J Nutr 137:1656S-1661S. 2007....
- The dogfish shark (Squalus acanthias) increases both hepatic and extrahepatic ornithine urea cycle enzyme activities for nitrogen conservation after feedingMakiko Kajimura
Department of Biology, McMaster University, Hamilton, Ontario, Canada
Physiol Biochem Zool 79:602-13. 2006..Furthermore, exogenous nitrogen from food is converted into urea not only by the liver but also by the muscle and to a small extent by the intestine...
- Neonatal pulmonary hypertension--urea-cycle intermediates, nitric oxide production, and carbamoyl-phosphate synthetase functionD L Pearson
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
N Engl J Med 344:1832-8. 2001....
- Ornithine transcarbamylase and arginase I deficiency are responsible for diminished urea cycle function in the human hepatoblastoma cell line HepG2Demetra Mavri-Damelin
The UCL Institute of Hepatology, Hampstead Campus, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
Int J Biochem Cell Biol 39:555-64. 2007..This strategy could provide a cell source capable of urea synthesis, and hence ammonia detoxificatory function, which would be useful in a bio-artificial liver...
- Lamprey parasitism of sharks and teleosts: high capacity urea excretion in an extant vertebrate relicMichael P Wilkie
Department of Biology, Mount Allison University, Sackville, NB, Canada E4L 1E4
Comp Biochem Physiol A Mol Integr Physiol 138:485-92. 2004....
- Differentially expressed genes associated with hepatitis B virus HBx and MHBs protein function in hepatocellular carcinomaDae Ghon Kim
Division of GI and Hepatology, Department of Internal Medicine, Chonbuk National University Medical School and Hospital, Jeonbuk, Republic of Korea
Methods Mol Biol 317:141-55. 2006..secretory trypsin inhibitor (PSTI), H19, guanidine nucleotide-binding protein alpha-1 subunit (GNAZ), carbamyl phosphate synthetase I (CPS I), insulin-like growth factor (IGF)-II, and 10 ribosomal proteins genes...
- Relationship between carbamoyl-phosphate synthetase genotype and systemic vascular functionMarshall L Summar
Department of Pediatrics, Division of Medical Genetics, Vanderbilt University Medical Center, Nashville, Tenn 37232 6602, USA
Hypertension 43:186-91. 2004..943). These data indicate that a polymorphism in the gene encoding carbamoyl-phosphate synthetase 1 influences nitric oxide production as well as vascular smooth muscle reactivity...
- [A case of late-onset carbamoyl phosphate synthetase I deficiency, presenting periodic psychotic episodes coinciding with menstrual periods]Y Wakutani
Department of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University
Rinsho Shinkeigaku 41:780-5. 2001..Genomic DNA analysis showed that she was heterozygous in V1149G mutation. The mutation allele was derived from her father. The causative factor for absence or very low level of maternal CPSI mRNA will require further analysis...
- Ornithine carbamoyltransferase from the extreme thermophile Thermus thermophilus--analysis of the gene and characterisation of the proteinR Sanchez
Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Brussels, Belgium
Eur J Biochem 248:466-74. 1997..In the presence of its substrates the half-life time was several hours at 85 degrees C. Ionic and hydrophobic interactions contribute to the stability. The expression of T. thermophilus OTC was negatively regulated by arginine...
- Integration host factor (IHF) modulates the expression of the pyrimidine-specific promoter of the carAB operons of Escherichia coli K12 and Salmonella typhimurium LT2D Charlier
Research Institute, CERIA COOVI, Brussels, Belgium
Mol Gen Genet 237:273-86. 1993..IHF thus appears to be required for maximal expression as well as for establishment of full repression. IHF could exert this function by modulating the binding of a pyrimidine-specific regulatory molecule...
- Cell cycle-dependent regulation of pyrimidine biosynthesisFrederic D Sigoillot
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
J Biol Chem 278:3403-9. 2003..Thus, the cell cycle-dependent regulation of pyrimidine biosynthesis results from the sequential phosphorylation and dephosphorylation of CAD under the control of two important signaling cascades...
- Urea cycle enzymes through the development of pacu (Piaractus mesopotamicus): the role of ornithine carbamoyl transferasePaulo Sérgio Monzani
Laboratory of Adaptive Biochemistry, Department of Genetics and Evolution, Federal University of Sao Carlos, Rod Washington Luiz Km 235, CP 676, CEP 13565 905, Sao Carlos, SP, Brazil
Fish Physiol Biochem 34:139-49. 2008..We suppose that OCT during the embryogenesis is a control step regulating the cellular concentration of ornithine for polyamines synthesis...
- Population screening in a Druze community: the challenge and the rewardTzipora C Falik-Zaccai
Institute of Human Genetics, Western Galilee Hospital Nahariya, Israel
Genet Med 10:903-9. 2008..of metabolism cerebrotendinous xanthomatosis, prolidase deficiency, argininosuccinate lyase deficiency, and carbamyl phosphate synthetase I deficiency were identified in an isolated Druze village in northern Israel...
- Effect of alkalinity (pH 10) on ureogenesis in the air-breathing walking catfish, Clarias batrachusNirmalendu Saha
Biochemical Adaptation Laboratory, Department of Zoology, North Eastern Hill University, Shillong 93 022, India
Comp Biochem Physiol A Mol Integr Physiol 132:353-64. 2002..The activity of glutamine synthetase and three enzymes operating in the urea cycle (carbamyl phosphate synthetase, argininosuccinate synthetase, argininosuccinate lyase) increased significantly in hepatic and ..
- Growth factor-regulated expression of enzymes involved in nucleotide biosynthesis: a novel mechanism of growth factor actionM G Gassmann
Max Planck Institute of Biochemistry, Martinsried, Germany
Oncogene 18:6667-76. 1999....
- Role of ureogenesis in the mud-dwelled Singhi catfish (Heteropneustes fossilis) under condition of water shortageN Saha
Biochemical Adaptation Laboratory, Department of Zoology, North Eastern Hill University, 793022, Shillong, India
Comp Biochem Physiol A Mol Integr Physiol 128:137-46. 2001..The activities (units/g tissue and /mg protein) of glutamine synthetase and three enzymes of the urea cycle, carbamyl phosphate synthetase, argininosuccinate synthetase and argininosuccinate lyase increased significantly in most of the ..
- Organization of carbamoyl-phosphate synthase genes in Neisseria gonorrhoeae includes a large, variable intergenic sequence which is also present in other Neisseria speciesF S Lawson
Department of Biology, University of Ottawa, Ontario, Canada
Microbiology 141:1183-91. 1995..meningitidis and 18 commensal Neisseria isolates comprising nine species. This unexpected organization of the CPS genes in N. gonorrhoeae is therefore widespread throughout the genus Neisseria...
- A comparison of the effects of environmental ammonia exposure on the Asian freshwater stingray Himantura signifer and the Amazonian freshwater stingray Potamotrygon motoroYuen K Ip
Department of Biological Science, National University of Singapore, Kent Ridge, Singapore 117543, Republic of Singapore
J Exp Biol 206:3625-33. 2003..Our results suggest that such a strategy began to develop in those elasmobranchs, e.g. H. signifer, that migrate into a freshwater environment from the sea but not in those permanently adapted to a freshwater environment...
- Structure and catalytic mechanism of a novel N-succinyl-L-ornithine transcarbamylase in arginine biosynthesis of Bacteroides fragilisDashuang Shi
Children s Research Institute, Children s National Medical Center, George Washington University, Washington, D C 20010, USA
J Biol Chem 281:20623-31. 2006..Crystal structures of this novel transcarbamylase complexed with carbamyl phosphate and N-succinyl-L-norvaline, as well as sulfate and N-succinyl-L-norvaline have been determined and refined ..
- Genetic variation in the mitochondrial enzyme carbamyl-phosphate synthetase I predisposes children to increased pulmonary artery pressure following surgical repair of congenital heart defects: a validated genetic association studyJeffrey A Canter
Center for Human Genetics Research, Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN, USA
Mitochondrion 7:204-10. 2007..In conclusion, the CPSI T1405N genotype appears to be an important new factor in predicting susceptibility to increased PAP following surgical repair of congenital cardiac defects in children...
- Urea synthesis in the African lungfish Protopterus dolloi--hepatic carbamoyl phosphate synthetase III and glutamine synthetase are upregulated by 6 days of aerial exposureShit F Chew
Natural Sciences, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore
J Exp Biol 206:3615-24. 2003..dolloi possesses transporters that facilitate the excretion of urea in water...
- Effects of crowding on ornithine-urea cycle enzyme mRNA expression and activity in gulf toadfish (Opsanus beta)Tammy Laberge
Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Science, University of Miami, 4600 Rickenbacker Causeway, Miami, FL 33149, USA
J Exp Biol 212:2394-402. 2009..These results suggest that the activities of O-UC enzymes are better predictors for urea production than O-UC enzyme mRNA expression levels...
- Converting catabolic ornithine carbamoyltransferase to an anabolic enzymeH Baur
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, Zurich, Switzerland
J Biol Chem 265:14728-31. 1990..coli; the hybrid enzyme had a markedly reduced homotropic cooperativity. This gene fusion method should be generally useful for directed enzyme evolution...
- The use of nucleotide analogs to evaluate the mechanism of the heterotropic response of Escherichia coli aspartate transcarbamoylaseJ B Sakash
Department of Chemistry, Merkert Chemistry Center, Boston College, Massachusetts 02467, USA
Protein Sci 9:53-63. 2000....
- The effects of various inhibitors on the regulation of orotic acid excretion in sparse-fur mutant mice (spf/Y) deficient in ornithine transcarbamylaseJ Nelson
Department of Nutrition, University of Montreal, Quebec, Canada
Chem Biol Interact 89:35-47. 1993..The orotic aciduria remained insensitive to acivicin, indicating mitochondria as the source of carbamyl phosphate. However, orotate excretion was significantly decreased by PALA (P < 0...
- Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetaseSatu Pekkala
Centro de Investigacion Principe Felipe, Avda Autopista del Saler 16, Valencia 46012, Spain
Biochem J 424:211-20. 2009....
- 3-isobutylmethylxanthine inhibits hepatic urea synthesis: protection by agmatineItzhak Nissim
Children s Hospital of Philadelphia, Division of Child Development, Department of Pediatrics, University of Pennsylvania School of Medicine, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
J Biol Chem 283:15063-71. 2008..Together, the data may suggest a new experimental application of IBMX in studies of CPS-I malfunction and the use of agmatine as intervention therapy...
- A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylaseDashuang Shi
Children s National Medical Center, The George Washington University, Washington, DC 20010, USA
Protein Sci 16:1689-99. 2007Transcarbamylases catalyze the transfer of the carbamyl group from carbamyl phosphate (CP) to an amino group of a second substrate such as aspartate, ornithine, or putrescine...
- Substrate binding and catalysis in carbamate kinase ascertained by crystallographic and site-directed mutagenesis studies: movements and significance of a unique globular subdomain of this key enzyme for fermentative ATP production in bacteriaSantiago Ramon-Maiques
Instituto de Biomedicina de Valencia Consejo Superior de Investigaciones Científicas and Centro de Investigación Biomédica en Red de Enfermedades Raras Instituto de Salud Carlos III, Jaime Roig 11, Valencia 46010, Spain
J Mol Biol 397:1261-75. 2010..It is concluded that the PSD movement allows CK to select against the abundant CP/carbamate analogues acetylphosphate/acetate and bicarbonate, rendering CK highly selective for CP/carbamate...
- Genomic organization of the human gene (CA5) and pseudogene for mitochondrial carbonic anhydrase V and their localization to chromosomes 16q and 16pY Nagao
Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, Missouri 63104, USA
Genomics 28:477-84. 1995..FISH analysis allowed CA5 to be assigned to chromosome 16q24.3. An unprocessed pseudogene containing sequence homologous to exons 3-7 and introns 3-6 was also isolated and was assigned by FISH analysis to chromosome 16p11.2-p12...
- Shifting patterns of nitrogen excretion and amino acid catabolism capacity during the life cycle of the sea lamprey (Petromyzon marinus)Michael P Wilkie
Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, N2L 3C5, Canada
Physiol Biochem Zool 79:885-98. 2006..The subsequent generation of energy-rich carbon skeletons can then be oxidized or retained for glycogen and fatty acid synthesis, which are essential fuels for the upstream migratory and spawning phases of the sea lamprey's life cycle...
- Adenovirus preterminal protein binds to the CAD enzyme at active sites of viral DNA replication on the nuclear matrixP C Angeletti
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham, 35294 0005, USA
J Virol 72:2896-904. 1998..Amino acid sequencing of the 150-kDa band revealed sequence identity to carbamyl phosphate synthetase I (CPS I) and a high degree of homology to the related trifunctional enzyme known as CAD (for ..
- Nitrogen metabolism in the African lungfish (Protopterus dolloi) aestivating in a mucus cocoon on landShit F Chew
Natural Sciences, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore
J Exp Biol 207:777-86. 2004..Our results indicate that a reduction in ammonia production and decreases in hepatic arginine and cranial tryptophan contents are important facets of aestivation in P. dolloi...
- The amidotransferase family of enzymes: molecular machines for the production and delivery of ammoniaF M Raushel
Department of Chemistry, Texas A and M University, College Station 77843, USA
Biochemistry 38:7891-9. 1999..Molecular tunnels between distant active sites have also been identified in tryptophan synthase and glutamine phosphoribosyl pyrophosphate amidotransferase and are likely architectural features in an expanding list of enzymes...
- Opposite effects on regulation of urea synthesis by early and late uraemia in ratsSusanne Schouw Nielsen
Department of Medicine V Hepatology and Gastroenterology, Aarhus University Hospital, 44 Noerrebrogade, DK 8000 Aarhus C, Denmark
Clin Nutr 26:245-51. 2007..The aims were to investigate the effects of early and late experimental renal failure on the regulation of hepatic urea synthesis and the expression of urea cycle enzyme genes in the liver...
- Crystal structure of a transcarbamylase-like protein from the anaerobic bacterium Bacteroides fragilis at 2.0 A resolutionDashuang Shi
Children s Research Institute, Children s National Medical Center, 111 Michigan Avenue N W, Washington, DC 20010 2970, USA
J Mol Biol 320:899-908. 2002..A similar protein has been found in Xylella fastidiosa, a bacterium that infects grapes, citrus and other plants...
- Genetic variation in the urea cycle: a model resource for investigating key candidate genes for common diseasesSabrina Mitchell
Center for Human Genetics Research, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Hum Mutat 30:56-60. 2009..Screening all the genes in the pathway provides a catalog of variants that can be used in investigating candidate diseases...
- Activation by fusion of the glutaminase and synthetase subunits of Escherichia coli carbamyl-phosphate synthetaseH I Guy
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
J Biol Chem 272:19913-8. 1997Escherichia coli carbamyl-phosphate synthetase consists of two subunits that act in concert to synthesize carbamyl phosphate. The 40-kDa subunit is an amidotransferase (GLN subunit) that hydrolyzes glutamine and transfers ammonia to the ..
- Non-radioactive detection of palmitoylated mitochondrial proteins using an azido-palmitate analogueMorris A Kostiuk
Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
Methods Enzymol 457:149-65. 2009..Recently, we used this labeling method, combined with mass spectrometry analysis of the labeled proteins, to identify 21 palmitoylated proteins from rat liver mitochondria...
- Somatotropin-induced amino acid conservation in pigs involves differential regulation of liver and gut urea cycle enzyme activityJill A Bush
U S Department of Agriculture Agricultural Research Service Children s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
J Nutr 132:59-67. 2002..The effect of ST treatment on porcine urea cycle enzymes is tissue-specific and is associated with a reduction in substrate availability for hepatic ureagenesis...
- Different alleles of the response regulator gene bldM arrest Streptomyces coelicolor development at distinct stagesV Molle
Department of Molecular Microbiology, John Innes Centre, Colney, Norwich, UK
Mol Microbiol 36:1265-78. 2000..bldM was not phosphorylated in vitro with either of the small-molecule phosphodonors acetyl phosphate or carbamoyl phosphate under conditions in which a control response regulator protein, NtrC, was labelled efficiently...
- Repression of the pyr operon in Lactobacillus plantarum prevents its ability to grow at low carbon dioxide levelsHerve Nicoloff
Laboratoire de Dynamique, Evolution et Expression de Génomes de Microorganismes, Université Louis Pasteur CNRS FRE 2326, 28 rue Goethe, F 67083 Strasbourg, France
J Bacteriol 187:2093-104. 2005..Therefore, growth inhibition without CO2 enrichment may be due to low carbamoyl phosphate pools from lack of CPS activity...
- Gene array analysis of Yersinia enterocolitica FlhD and FlhC: regulation of enzymes affecting synthesis and degradation of carbamoylphosphateVinayak Kapatral
Integrated Genomics, Inc, 2201 West Campbell Park Dr, Chicago, IL 60612, USA
Microbiology 150:2289-300. 2004..These data are consistent with the hypothesis that temperature regulation of flagella genes might be mediated by the flagella-specific sigma factor FliA and not the flagella master regulator FlhD/FlhC...
- Effect of dietary carbamyl phosphate on dentine apposition in rat molarsJohn Leonora
Department of Physiology Pharmacology, Loma Linda University, Loma Linda, CA 92350, USA
Arch Oral Biol 47:147-53. 2002..In earlier work it was found that carbamyl phosphate could significantly reverse sucrose-induced cariogenesis and also stimulate sucrose-depressed movement of ..
- Carbonic anhydrase inhibitors. Interaction of isozymes I, II, IV, V, and IX with phosphates, carbamoyl phosphate, and the phosphonate antiviral drug foscarnetStefano Rusconi
Istituto di Malattie Infettive e Tropicali, Universita degli Studi di Milano, Ospedale Luigi Sacco, Via G B Grassi 74, 20157 Milano, Italy
Bioorg Med Chem Lett 14:5763-7. 2004..The transmembrane, tumor-associated isozyme hCA IX was on the other hand slightly inhibited by all these anions...
- MECHANISM AND CONTROL OF UREA BIOSYNTHESISFrank M Raushel; Fiscal Year: 2011..These studies may lead to the development of new antibiotics in the defense of bacterial infections. ..
- REGULATION OF 15N UREA ISOTOPOMERS PRODUCTIONItzhak Nissim; Fiscal Year: 2013..Our preliminary results suggest that such treatment is feasible. The outcome of the propose study may provide new guidelines for evaluation, diagnosis and treatment of defective urea synthesis in NAFLD or MS. ..
- REGULATION OF 15N UREA ISOTOPOMERS PRODUCTIONItzhak Nissim; Fiscal Year: 2009..and disease states and thus may lead to development of a novel intervention to improve urea synthesis and detoxification of ammonia associated with fatty liver or in cases such as drug-induced inhibition of urea synthesis ..
- The molecular bases of inherited urea cycle disorders and ureagenesis regulationMendel Tuchman; Fiscal Year: 2010..evidence for multiple NAGS proteins in the liver and for in vivo and in vitro interactions between NAGS and carbamyl phosphate synthetase I (CPSI)...
- Rare Diseases Clinical Research Consortia (RDCRC) for the RDCR NetworkMark L Batshaw; Fiscal Year: 2013..six enzymes and two membrane transporters involved in urea biosynthesis: N-acetylglutamate synthase (NAGS);Carbamyl phosphate synthase I (CPSI) deficiency;Ornithine transcarbamylase deficiency (OTCD);Argininosuccinate synthase (AS) ..
- Genotype Analysis for Diagnosis of Urea Cycle DisordersSteven Dobrowolski; Fiscal Year: 2007..Products of the following genes are required for ureagenesis: N-acetylglutamate synthetase carbamyl phosphate synthetase 1 , ornithine transcarbamylase , argininosuccinate synthetase , argininosuccinate lyase , ..
- T. Gondii: Pyrimidine Synthesis as a Chemo TargetDavid J Bzik; Fiscal Year: 2013..Consequently, this research project will accelerate the discovery of new vaccines and treatments for devastating opportunistic parasite infections in AIDS. ..
- Genotype Analysis for Diagnosis of Urea Cycle DisordersSteven Dobrowolski; Fiscal Year: 2005..The urea cycle contains 6 core genes: N-acetylglutamate synthetase, carbamyl phosphate synthetase, ornithine transcarbamylase (OTC), argininosuccinate synthetase, argininosuccinate lyase, and ..
- PHOSPHORUS-CONTAINING TRANSITION STATE ANALOGSPaul Bartlett; Fiscal Year: 1990..the inhibition of these enzymes will be developed and applied to the synthesis of multisubstrate analogs for carbamyl phosphate synthetase and phosphoribosyl pyrophosphate amidotransferase. E...
- N-carbamylglutamate in the treatment of hyperammonemiaMendel Tuchman; Fiscal Year: 2013..hyperammonemia with N-carbamyl-L-glutamate (NCG) in propionic acidemia (PA), methylmalonic acidemia (MMA), carbamyl phosphate synthetase 1 deficiency (CPSD) and ornithine transcarbamylase deficiency (OTCD) changes the clinical outcome ..
- MOLECULAR GENETIC STUDIES OF UREA CYCLE DISORDERSJANICE FINKELSTEIN; Fiscal Year: 1990..This application focuses on disorders of ornithine transcarbamylase (OTC) and carbamyl phosphate synthetase (CPS)...
- ORGANIZATION AND REGULATION OF THE ATCASE CISTRONSJames Wild; Fiscal Year: 1993....
- CONTROL OF PYRIMIDINE BIOSYNTHESIS IN MAMMALIAN CELLSDavid Evans; Fiscal Year: 1990..The first three enzymes of the pathway, carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase are carried by a single 230 Kd polypeptide chain...
- CARBON DIOXIDE EXCHANGES IN THE LIVERSUSANNA DODGSON; Fiscal Year: 1991..of CAmit is to provide the substrate HCO3- for the first urea cycle enzyme, the mitochondrially located carbamyl phosphate synthetase I. We will determine if there are any other effects of the sulfonamides on the mitochondria...
- Structure-Function Analysis of EnzymesHazel Holden; Fiscal Year: 2004....
- CATALYSIS AND REGULATION OF THE UREA CYCLESUSAN POWERS LEE; Fiscal Year: 2001..Proposed specific aims 4 and 5 are intended to elucidate the structural and functional routes of communication among the various domains of CPS that allow its catalysis and regulation. ..
- PURCHASE OF 500 MHZ NMR SPECTROMETERFRANK RAUSHEL; Fiscal Year: 1991..The structures of novel amino acid and peptide mimics from controlled radical reactions are being investigated by the Newcomb laboratory...
- REGULATION OF UREA CYCLE ENZYME SYNTHESISSidney Morris; Fiscal Year: 1991..This grant proposes to isolate and characterize genomic DNA clones encoding two of the urea cycle enzymes -- carbamyl phosphate synthetase I and argininosuccinate synthetase...
- STRUCTURE/FUNCTION OF CARBAMYL PHOSPHATE SYNTHETASECAROL LUSTY; Fiscal Year: 2001..coli enzyme, glutamine-dependent carbamyl phosphate synthetase (CPS)...
- Chemotherapy Toxicity: Reduction via Urea Cycle SupportBrian Christman; Fiscal Year: 2004..We have also discovered a novel, population-based, exonic, single nucleotide polymorphism (SNP) in carbamyl phosphate synthetase-1 (CPS-1), the rate limiting enzyme in urea cycle function...
- Discodermolide Biosynthesis GenesCharles Hutchinson; Fiscal Year: 2002..Our research will also establish the technological base for a general approach to making many other currently scarce marine polyketides with anti-tumor activity available in large amounts for drug development. ..
- Mechanisms and Control of Urea BiosynthesisFRANK RAUSHEL; Fiscal Year: 2007..abstract_text> ..
- PORPHYRIN AND CORRINOID BIOSYNTHESISFRANK RAUSHEL; Fiscal Year: 2008..Finally, the multi-enzyme synthesis of advanced intermediates and of B12 itself will be addressed. ..
- ISOTOPIC PROBES OF ENZYME REACTION MECHANISMSFRANK RAUSHEL; Fiscal Year: 2005..These investigations will complement concurrent efforts to direct the evolution of new and enhanced activities from present-day proteins. ..
- Structural Studies of NAGSDashuang Shi; Fiscal Year: 2006..abstract_text> ..
- COMMON POLYMORPHISM EFFECTS ON UREA CYCLE FUNCTIONMarshall Summar; Fiscal Year: 2001..My research has focused on characterizing the gene encoding the first, rate-limiting step of ureagenesis, carbamyl phosphate synthetase I (CPSI)...
- MITOCHONDRIAL CYTOCHROME C RELEASE VIA MEMBRANE PORESElliott Crouser; Fiscal Year: 2004..These advisors are actively investigating the role mitochondria in the pathogenesis of disease. Thus, the prospects for successful collaborative initiatives, a critical determinant of future success, are excellent. ..
- PREVENTION OF IFOSFAMIDE INDUCED NEPHROTOXICITYItzhak Nissim; Fiscal Year: 2004..abstract_text> ..
- Structural Studies of the Pyrimidine PathwayJacqueline Vitali; Fiscal Year: 2004..Finally, understanding the means of extreme heat stability will allow us to engineer selected features in other aspartate transcarbamoylases to obtain enzymes with enhanced thermostability. ..
- REGULATION OF CARBAMYL PHOSPH SYNTHETASES BY MAP KINASELee Graves; Fiscal Year: 2002..CAD is composed of three enzymes carbamyl phosphate synthetase (CPS II), aspartate transcarbamylase and dihydrooratase...
- THERMODYNAMICS OF BIOCHEMICAL SYSTEMSROBERT ALBERTY; Fiscal Year: 2005..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
- Stromal Gene Expression During Pulmonary SarcoidosisElliott Crouser; Fiscal Year: 2005..Statistical comparisons of gene expression patterns will then be performed. Novel insights relating gene profiles to disease manifestation, prognosis and pathogenesis are likely outcomes of these investigations. ..
- Induction of Inflammation by Mitochondrial ProteinsElliott Crouser; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Iron as a Nutritional Modifier of Toxic Neuropathy in HIV/AIDSAsha Kallianpur; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Regulation of Nucleoside Transporters by Protein KinasesLee Graves; Fiscal Year: 2007..Aim #2. Elucidate the promoter elements necessary for the expression of ENT1 and ENT2. Aim #3. Examine the specific roles of ENTs in growth, differentiation and chemosensitization . ..