p chloromercuribenzoic acid

Summary

Summary: An organic mercurial used as a sulfhydryl reagent.

Top Publications

  1. ncbi Partial purification and kinetic properties of three different D-glucosamine 6-P:N-acetyltransferase forms from human placenta
    M Vessal
    Department of Biochemistry, Shiraz University of Medical Sciences, Iran
    Comp Biochem Physiol B Biochem Mol Biol 121:379-84. 1998
  2. ncbi Critical role of the cysteine 323 residue in the catalytic activity of human glutamate dehydrogenase isozymes
    Seung Ju Yang
    Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138 736, Korea
    Mol Cells 19:97-103. 2005
  3. ncbi Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus
    Hosam E Ewis
    Department of Biology, Georgia State University, 24 Peachtree Center Avenue, Atlanta, GA 30303, USA
    Gene 329:187-95. 2004
  4. ncbi Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168
    Qing Zhang
    The Key Laboratory of Gene Engineering of Ministry of Education, Sun Yet Sen University, Guangzhou 510275, PR China
    FEMS Microbiol Lett 228:39-44. 2003
  5. ncbi Sensitivity of lysosomal enzymes to the plant alkaloid sanguinarine: comparison with other SH-specific agents
    T Belyaeva
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Cell Biol Int 27:887-95. 2003
  6. ncbi Simultaneous modulation of transport and metabolism of acyclovir prodrugs across rabbit cornea: An approach involving enzyme inhibitors
    Suresh Katragadda
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri Kansas City, 5005 Rockhill Road, Kansas City, MO 64110 2499, USA
    Int J Pharm 320:104-13. 2006
  7. ncbi Diffusion pathways to critical cysteines in the vesicular acetylcholine transporter of Torpedo
    James E Keller
    Department of Chemistry and Biochemistry, The Neuroscience Research Institute, University of California, Santa Barbara, California 93106, USA
    Neurochem Res 28:477-82. 2003
  8. ncbi Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange
    Judith Lahav
    Coagulation Laboratory, Institute of Hematology, Rabin Medical Center Beilinson Campus, Petah Tiqva, Israel
    Blood 100:2472-8. 2002
  9. pmc The reaction of iodine and thiol-blocking reagents with human complement components C2 and factor B. Purification and N-terminal amino acid sequence of a peptide from C2a containing a free thiol group
    C Parkes
    Biochem J 213:201-9. 1983
  10. ncbi Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biochem 132:967-73. 2002

Scientific Experts

Detail Information

Publications154 found, 100 shown here

  1. ncbi Partial purification and kinetic properties of three different D-glucosamine 6-P:N-acetyltransferase forms from human placenta
    M Vessal
    Department of Biochemistry, Shiraz University of Medical Sciences, Iran
    Comp Biochem Physiol B Biochem Mol Biol 121:379-84. 1998
    ..The results are discussed in terms of differences among the enzyme properties of human placental, rodent and porcine liver forms...
  2. ncbi Critical role of the cysteine 323 residue in the catalytic activity of human glutamate dehydrogenase isozymes
    Seung Ju Yang
    Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138 736, Korea
    Mol Cells 19:97-103. 2005
    ..There were no differences between the two hGDH isozymes in sensitivities to mutagenesis at C323. Our results suggest that C323 plays an important role in catalysis by human GDH isozymes...
  3. ncbi Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus
    Hosam E Ewis
    Department of Biology, Georgia State University, 24 Peachtree Center Avenue, Atlanta, GA 30303, USA
    Gene 329:187-95. 2004
    ..The enzymatic properties of Est30 and Est55 reported here warrant the potential applications of these enzymes in biotechnological industries...
  4. ncbi Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168
    Qing Zhang
    The Key Laboratory of Gene Engineering of Ministry of Education, Sun Yet Sen University, Guangzhou 510275, PR China
    FEMS Microbiol Lett 228:39-44. 2003
    ..The purified enzyme hydrolyzed various N-methylcarbamate insecticides. Carbaryl is the preferred substrate...
  5. ncbi Sensitivity of lysosomal enzymes to the plant alkaloid sanguinarine: comparison with other SH-specific agents
    T Belyaeva
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Cell Biol Int 27:887-95. 2003
    ..Thus, both the experimental observations obtained in this study and the literature data imply a significant role of redox-based mechanisms in regulating lysosomal functional activity...
  6. ncbi Simultaneous modulation of transport and metabolism of acyclovir prodrugs across rabbit cornea: An approach involving enzyme inhibitors
    Suresh Katragadda
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri Kansas City, 5005 Rockhill Road, Kansas City, MO 64110 2499, USA
    Int J Pharm 320:104-13. 2006
    ..In conclusion, utility of enzyme inhibitors to modulate transport and metabolism of prodrugs appears to be promising strategy for enhancing drug transport across cornea...
  7. ncbi Diffusion pathways to critical cysteines in the vesicular acetylcholine transporter of Torpedo
    James E Keller
    Department of Chemistry and Biochemistry, The Neuroscience Research Institute, University of California, Santa Barbara, California 93106, USA
    Neurochem Res 28:477-82. 2003
    ..The results suggest that hydrophobic and electrostatic barriers inhibit modification of Torpedo VAChT by negatively charged organomercurials and glutathione cannot reach cysteine 2 from either side of the membrane...
  8. ncbi Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange
    Judith Lahav
    Coagulation Laboratory, Institute of Hematology, Rabin Medical Center Beilinson Campus, Petah Tiqva, Israel
    Blood 100:2472-8. 2002
    ....
  9. pmc The reaction of iodine and thiol-blocking reagents with human complement components C2 and factor B. Purification and N-terminal amino acid sequence of a peptide from C2a containing a free thiol group
    C Parkes
    Biochem J 213:201-9. 1983
    ..Automated Edman degradation of the peptide showed that it was the N-terminal peptide of C2a. The free thiol group was identified at position 18...
  10. ncbi Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biochem 132:967-73. 2002
    ..Chemical modification studies of the CPY-I(C) complex with specific reagents demonstrated that the catalytic Ser146 and S1 substrate-binding site of CPY are covered in the complex...
  11. pmc Na self inhibition of human epithelial Na channel: temperature dependence and effect of extracellular proteases
    Ahmed Chraibi
    Institut de Pharmacologie et de Toxicologie, CH 1005 Lausanne, Switzerland
    J Gen Physiol 120:133-45. 2002
    ..The extracellular Na(+)-dependent inactivation has a large energy of activation and can be abolished by treatment with extracellular proteases...
  12. ncbi Purification and some properties of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1
    Shinji Mitsuiki
    Department of Industrial Chemistry, Kyushu Sangyo University, Fukuoka, Japan
    Biosci Biotechnol Biochem 66:164-7. 2002
    ..Strain TOA-1 produced a variety of alkaline hydrolytic enzymes. An alkaline protease, designated NAPase, was purified and characterized. NAPase had a very high keratinolytic activity and high stability under acidic conditions...
  13. pmc Repacking of the transmembrane domains of P-glycoprotein during the transport ATPase cycle
    M F Rosenberg
    Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, UK
    EMBO J 20:5615-25. 2001
    ..Biochemical data suggest that these rearrangements may involve rotation of transmembrane alpha-helices. A mechanism for transport is suggested...
  14. ncbi [Effect of p-chloromercuribenzoic acid and dithiothreitol on the Ca(2+) content of salivary glands and their protein secretion]
    N V Fedirko
    Ivan Franko Nataional University of Lviv
    Fiziol Zh 47:35-41. 2001
    ..Finally, we confirm important functional role of SH-groups in the regulation of Ca(2+)-homeostasis in secretory cells of exocrine glands...
  15. ncbi Identification of residues in the translocation pathway of EmrE, a multidrug antiporter from Escherichia coli
    M Lebendiker
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 271:21193-9. 1996
    ..The results demonstrate the existence of a domain accessible only to substrates and provide a unique tool for studying the substrate permeation pathway of an ion-coupled transporter...
  16. ncbi Isolation and characterization of mitochondrial F(1)-ATPase from crayfish (Orconectes virilis) gills
    Z Li
    Mount Sinai School of Medicine, Department of Human Genetics, 1425 Madison Ave, New York, NY 10029, USA
    Comp Biochem Physiol B Biochem Mol Biol 128:325-38. 2001
    ..In addition, an unmodified F(0) stabilizes F(1) and decreases F(1) sensitivities to inhibitors and modulators...
  17. ncbi Functional activity of a monocarboxylate transporter, MCT1, in the human retinal pigmented epithelium cell line, ARPE-19
    Soumyajit Majumdar
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri Kansas City, 5005 Rockhill Road, Kansas City, MO 64110, USA
    Mol Pharm 2:109-17. 2005
    ..This cell line may thus be used as an effective screening tool for intravitreally administered drugs/prodrugs targeted toward MCT1 expressed on the RPE...
  18. ncbi The role of monocarboxylate transporter 2 and 4 in the transport of gamma-hydroxybutyric acid in mammalian cells
    Qi Wang
    Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Amherst, NY 14260, USA
    Drug Metab Dispos 35:1393-9. 2007
    ..This investigation demonstrated that GHB is a substrate for both MCT2 and MCT4; these transporters may be important in the nonlinear disposition of GHB, as well as influencing its tissue distribution...
  19. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
    ..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
  20. ncbi The role of monocarboxylate transporters in uptake of lactic acid in HeLa cells
    Sravanthi Cheeti
    Division of Pharmaceutical Sciences, University of Missouri, School of Pharmacy, 5005 Rock Hill Road, Kansas City, MO 64110 2499, USA
    Int J Pharm 325:48-54. 2006
    ..MCT isoforms 1, 5 and 6 in HeLa cells were identified by RT-PCR. HeLa cell line can be used as an effective screening tool for intravaginally administered drugs targeted toward MCT...
  21. ncbi Monocarboxylate transporter 1 (MCT1) mediates transport of short-chain fatty acids in bovine caecum
    Doaa Kirat
    Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069 8501, Japan
    Exp Physiol 91:835-44. 2006
    ..The results show that MCT1 is a major route for SCFA efflux across the basolateral membrane of bovine large intestine and that it could play a role in the regulation of intracellular pH...
  22. pmc The intracellular pathway of the acetylcholine-induced contraction in cat detrusor muscle cells
    J Y An
    Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul, 156 756, Republic of Korea
    Br J Pharmacol 137:1001-10. 2002
    ..5. These results suggest that in cat detrusor muscle contraction induced by ACh is mediated via M3 muscarinic receptor-dependent activation of Gq/11 and PLC-beta1 and IP3-dependent Ca(2+) release...
  23. ncbi The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolution
    I P Korndörfer
    Institute of Molecular Biology Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, OR, 97403 1229, USA
    J Mol Biol 300:917-33. 2000
    ..The available structural data suggest that a metal-mediated hydride-shift mechanism, which is generally favored for xylose isomerase, is also feasible for rhamnose isomerase...
  24. ncbi Inhibition of l-arginine transport in platelets by asymmetric dimethylarginine and N-monomethyl-l-arginine: effects of arterial hypertension
    Tmc Brunini
    Departamento de Farmacologia e Psicobiologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil
    Clin Exp Pharmacol Physiol 31:738-40. 2004
    ..It is possible that endogenous l-arginine analogues, by inhibiting NO synthesis, are involved in the platelet activation present in hypertension...
  25. ncbi [Effect of inhibitors of energy-dependent Ca2+-transporting systems on ATP-hydrolase activity of smooth muscle actomyosin]
    S A Kosterin
    Palladine Institute of Biochemistry, NAS of Ukraine, Kyiv
    Ukr Biokhim Zh 73:57-62. 2001
    ..The data obtained could be used for the further development of the ideas about regularities of Ca(2+)-dependent control of the smooth muscles contraction-relaxation...
  26. ncbi Purification and characterization of a recombinant listeriolysin O expressed in Escherichia coli and possible diagnostic applications
    Camilla Giammarini
    Centro di Biotecnologie, Universita degli Studi di Urbino, via T Campanella 1, 61032 Fano, Italy
    J Biotechnol 109:13-20. 2004
    ..Here we report the characterization of toxin properties and its preliminary application in an ELISA diagnostic test for listeriosis...
  27. ncbi Biochemical characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase)
    P F Weller
    J Biol Chem 259:15100-5. 1984
    ..1%, sodium dodecyl sulfate. The hydrophobic nature of this protein, which accounts for 10% of the protein of the eosinophil, may contribute to its unique propensity for crystallization in vivo...
  28. ncbi Role of cysteine residues in glutathione synthetase from Escherichia coli B. Chemical modification and oligonucleotide site-directed mutagenesis
    H Kato
    Institute for Chemical Research, Kyoto University, Japan
    J Biol Chem 263:11646-51. 1988
    ..Replacement of Cys-122 to Ala-122 enhanced the reactivity of Cys-289 with sulfhydryl reagents...
  29. ncbi X-ray diffraction of heavy-atom labelled two-dimensional crystals of rhodopsin identifies the position of cysteine 140 in helix 3 and cysteine 316 in helix 8
    Thorsten Mielke
    Freie Universitat Berlin, Biophysics Group, Department of Physics, Berlin, Germany
    J Mol Biol 316:693-709. 2002
    ....
  30. ncbi Existence of an iron-oxidizing bacterium Acidithiobacillus ferrooxidans resistant to organomercurial compounds
    Fumiaki Takeuchi
    Environmental Management and Safety Section, Health and Environment Center, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    J Biosci Bioeng 99:586-91. 2005
    ..The cytosol prepared from MON-1 could volatilize Hg0 from PCMB (0.015 nmol mg(-1) h(-1)), PMA (0.33 nmol mg(-1) h(-1)) and MMC (0.005 nmol mg(-1) h(-1)) in the presence of NADPH and beta-mercaptoethanol...
  31. ncbi Extracellular alpha-amylase from Thermus filiformis Ork A2: purification and biochemical characterization
    M C Egas
    Departamento de Bioquimica, Universidade de Coimbra, Portugal
    Extremophiles 2:23-32. 1998
    ..To our knowledge this is the first report of the characterization of an alpha-amylase from a strain of the genus Thermus...
  32. ncbi A novel alpha-glucosidase from the moss Scopelophila cataractae
    Yoshiki Yamasaki
    Research Institute for Bioresources, Okayama University, Okayama, Japan
    Acta Biochim Pol 54:401-6. 2007
    ..These results show that alpha-glucosidases should be capable of complete starch digestion by themselves in cells of S. cataractae...
  33. ncbi Purification and some properties of two enzymes from rat liver cytosol that catalyze carbonyl reduction of 6-tert-butyl-2, 3-epoxy-5-cyclohexene-1,4-dione, a metabolite of 3-tert-butyl-4-hydroxyanisole
    K Tajima
    Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, 920 1181, Japan
    Arch Biochem Biophys 361:207-14. 1999
    ..Dicoumarol inhibited significantly TBE-R1 activity but not TBE-R2 activity. In the conversion of TBE to TBEH, TBE-R1 preferentially reduced TBE to (4R)-TBEH, whereas TBE-R2 preferred the reduction of TBE to (4S)-TBEH...
  34. ncbi Volatilization and recovery of mercury from mercury-polluted soils and wastewaters using mercury-resistant Acidithiobacillus ferrooxidans strains SUG 2-2 and MON-1
    Fumiaki Takeuchi
    Environmental Management and Safety Section, Health and Environment Center, Okayama University, 3 1 1, Tsushima Naka, Okayama 700 8530, Japan
    Environ Sci 13:305-16. 2006
    ..5) containing 40 microM Hg(2+) and ferrous iron. The MON-1-immobilized PVA resins were used repeatedly...
  35. ncbi Differential metabolism of Tyr-MIF-1 and MIF-1 in rat and human plasma
    A J Kastin
    VA Medical Center, New Orleans, LA
    Biochem Pharmacol 47:699-709. 1994
    ....
  36. doi Identification of valproic acid glucuronide hydrolase as a key enzyme for the interaction of valproic acid with carbapenem antibiotics
    Eiko Suzuki
    Drug Metabolism and Pharmacokinetics Research Laboratories, Daiichi Sankyo Co, Ltd, 1 2 58 Hiromachi, Shinagawa ku, Tokyo, Japan
    Drug Metab Dispos 38:1538-44. 2010
    ..Taken together, APEH plays a major role in the PAPM-sensitive VPA-G hydrolysis in the liver. These findings suggest that APEH could be a key enzyme for the drug interaction of VPA with carbapenems via VPA-G hydrolysis...
  37. ncbi Very long chain and long chain acyl-CoA thioesterases in rat liver mitochondria. Identification, purification, characterization, and induction by peroxisome proliferators
    L T Svensson
    Department of Metabolic Research, Wenner Gren Institute Arrhenius Laboratories F3, Stockholm University, Sweden
    J Biol Chem 270:12177-83. 1995
    ..Comparison of physical and catalytical characteristics of the enzymes studied here and previously purified acyl-CoA thioesterases suggest that MTE-I and MTE-II are novel enzymes...
  38. ncbi [Involvement of SH-groups in the regulation of adenylate cyclase signal system by isoproterenol and serotonin in cultured murine fibroblasts L (subline LSM)]
    A O Shpakov
    Institute of Evolutionary Physiology and Biochemistry RAS, St Petersburg
    Tsitologiia 43:1130-5. 2001
    ..The forms of SH-groups in proteins involved in the ACS determine their functional activities and a possibility of transduction of the hormonal signal on the effector systems...
  39. ncbi The multiple site binding of carboxypeptidase Y inhibitor (IC) to the cognate proteinase. Implications for the biological roles of the phosphatidylethanolamine-binding protein
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biol Chem 278:29792-8. 2003
    ..Considering the fact that mouse PEBP has recently been identified as a novel thrombin inhibitor, the binding that characterizes the CPY-IC complex could be a common feature of PEBPs...
  40. ncbi Characterization of an alkaline protease from Bacillus sp. no. AH-101
    H Takami
    Department of Microbial Ecology, Institute of Physical and Chemical Research, Saitama, Japan
    Appl Microbiol Biotechnol 33:519-23. 1990
    ..The no. AH-101 alkaline protease was compared with subtilisin BPN', subtilisin Carlsberg, no. 221, and Ya-B alkaline proteases. Extensive sequence homology existed among these enzymes...
  41. ncbi Purification and characterization of chitinase from Streptomyces sp. M-20
    Kyoung Ja Kim
    Department of Life Science, Soonchunhyang University, Asan si 336 745, Korea
    J Biochem Mol Biol 36:185-9. 2003
    ..The chitinase activity was high on colloidal chitin, chitotriose, and chitooligosaccharide. The purified chitinase showed antifungal activity against Botrytis cinerea, and lysozyme activity against the cell wall of Botrytis cinerea...
  42. ncbi Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4-carboxylic acid
    K Mochizuki
    National Institute of Bioscience and Human Technology, AIST, 1 1, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Arch Microbiol 172:182-5. 1999
    ..The enzyme required ATP, Mg(2+), and KCl for the cleavage of L-2-oxothiazolidine-4-carboxylic acid. The enzyme also cleaved 5-oxo-L-proline to L-glutamic acid and is considered to be 5-oxo-L-prolinase...
  43. ncbi Biliverdin-IX alpha reductase and biliverdin-IX beta reductase from human liver. Purification and characterization
    T Yamaguchi
    Department of Biochemical Genetics, Tokyo Medical and Dental University, Japan
    J Biol Chem 269:24343-8. 1994
    ..2 whereas that for isozymes III and IV was 7.4. The proportion of the total activity of isozymes I and II to that of isozymes III and IV was considerably higher in the fetal than in the adult liver...
  44. ncbi cDNA cloning of MCT2, a second monocarboxylate transporter expressed in different cells than MCT1
    C K Garcia
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235 9046
    J Biol Chem 270:1843-9. 1995
    ..These findings suggest that MCT1 and MCT2 are adapted to play different roles in monocarboxylate transport in different cells of the body...
  45. pmc Human brain contains a novel non-AT1, non-AT2 binding site for active angiotensin peptides
    Vardan T Karamyan
    Department of Pharmacology, School of Pharmacy, University of Mississippi, University, MS 38677, USA
    Life Sci 83:421-5. 2008
    ..To determine whether the novel non-AT1, non-AT2 binding site for angiotensins recently discovered in rodent brains occurs in the human brain...
  46. pmc Oxygen consumption rates and oxygen concentration in molt-4 cells and their mtDNA depleted (rho0) mutants
    Jiangang Shen
    EPR Center, Department of Diagnostic Radiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Biophys J 84:1291-8. 2003
    ..The results are consistent with our earlier findings suggesting that such oxygen gradients may be a general phenomenon found in most or all cell systems under appropriate conditions...
  47. ncbi Regional distribution and age-dependent expression of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain
    Jun Morishita
    Department of Biochemistry, Kagawa University School of Medicine, Kagawa, Japan
    J Neurochem 94:753-62. 2005
    ..These results demonstrated wide distribution of NAPE-PLD in various brain regions and its age-dependent expression, suggesting the central role of this enzyme in the formation of anandamide and other N-acylethanolamines in the brain...
  48. ncbi Isolation and characterization of novel long-chain acyl-CoA thioesterase/carboxylesterase isoenzymes from Candida rugosa
    M A Diczfalusy
    Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, Sweden
    Arch Biochem Biophys 334:104-12. 1996
    ..N-terminal sequence analysis and labeling by DFP suggest that these long-chain acyl-CoA thioesterases belong to a novel group of yeast serine esterases...
  49. ncbi Identification of a novel non-AT1, non-AT2 angiotensin binding site in the rat brain
    Vardan T Karamyan
    Department of Pharmacology and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA
    Brain Res 1143:83-91. 2007
    ..4.24.15 and/or EC 3.4.24.16. However, some unique characteristics of this non-AT1, non-AT2 binding site suggest that it may be a novel angiotensin binding substance...
  50. ncbi Cell death and cytotoxic effects in YAC-1 lymphoma cells following exposure to various forms of mercury
    Margaret Yole
    Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, University of Saskatchewan, Saskatoon SK S7N 5B4, Canada
    Toxicology 231:40-57. 2007
    ..These findings indicate the two groups of Hg compounds may induce cell death by distinct pathways, reflecting interactions with different cellular targets leading to cell death...
  51. ncbi Esterase patterns and phylogenetic relationships of Drosophila species in the saltans subgroup (saltans group)
    A P Nascimento
    Instituto de Biociencias, Letras e Ciencias Exatas UNESP, Sao Jose do Rio Preto, SP, Brazil
    Genetica 114:41-51. 2002
    ..septentriosaltans, D. saltans and D. austrosaltans are closer to each other than to D. prosaltans. These results showed to be consistent with phylogenetic relationships previously inferred from inversion polymorphism...
  52. ncbi Salt stress enhances choline uptake in the halotolerant cyanobacterium Aphanothece halophytica
    Aran Incharoensakdi
    Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand
    Biochim Biophys Acta 1621:102-9. 2003
    ..The results of competition studies suggested that N-methyl on one end of molecule and on the other end either an aldehyde, an alcohol or a neutral group were important features for substrate recognition...
  53. doi Brain angiotensin receptors and binding proteins
    Robert C Speth
    Department of Pharmacology, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, Oxford, MS 38677, USA
    Naunyn Schmiedebergs Arch Pharmacol 377:283-93. 2008
    ..g., stimulation of dopamine release from the striatum, as a peptidase, or as a clearance receptor, and the importance of the state of the internal environment of the brain to its function is reviewed...
  54. ncbi 2-(2'-Hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2: purification and characterization
    J Konishi
    Bio Refining Process Laboratory, Japan Cooperation Center, Petroleum, 1900 Sodeshi cho, 424 0037, Shimizu, Shizuoka, Japan
    Appl Microbiol Biotechnol 62:356-61. 2003
    ..TdsB desulfurized 2-(2'-hydroxyphenyl)ethen-1-sulfinate to yield o-hydroxystyrene, but DszB could not. A site-directed mutagenesis study revealed the cysteine residue at position 17 to be essential to the catalytic activity of TdsB...
  55. ncbi Isolation and characterization of a cysteine protease of freesia corms
    Tetsuya Uchikoba
    Kagoshima University Musium, Japan
    Biosci Biotechnol Biochem 66:448-52. 2002
    ..From the hydrolysis of peptidyl-p-NAs, the specificity of FP-A was found to be broad. It was thought that FP-A was a new protease from freesia corms...
  56. ncbi Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism
    Li Ying Zhao
    Department of Biochemistry, Kagawa University School of Medicine, 1750 1 Ikenobe, Miki, Kagawa 761 0793, Japan
    Biochim Biophys Acta 1771:1397-405. 2007
    ..The results exhibited that Asn-37, Asn-107, Asn-309, and Asn-333 are actual N-glycosylation sites. The glycosylation appeared to play an important role in stabilizing the enzyme protein...
  57. doi Characterization of the brain-specific non-AT(1), non-AT(2) angiotensin binding site in the mouse
    Vardan T Karamyan
    Department of Pharmacology, School of Pharmacy, University of Mississippi, University, MS 38677, United States
    Eur J Pharmacol 590:87-92. 2008
    ..Whether this binding site functions as a novel receptor for angiotensins or a specific angiotensinase with variable functionality at different redox states will require further study...
  58. ncbi Isolation and characterization of a new extracellular bacteriolytic endopeptidase of Lysobacter sp. XL1
    O A Stepnaya
    Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia
    Biochemistry (Mosc) 70:1031-7. 2005
    ..The enzyme is inhibited by p-chloromercuribenzoic acid by 72% and by phenylmethylsulfonyl fluoride by 43%, which indicates the involvement of serine and thiol groups in its functioning...
  59. pmc Purification and characterization of a novel thermostable alpha-L-arabinofuranosidase from a color-variant strain of Aureobasidium pullulans
    B C Saha
    Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, U S Department of Agriculture, Peoria, Illinois 61604, USA
    Appl Environ Microbiol 64:216-20. 1998
    ..2 M). The enzyme did not require a metal ion for activity, and its activity was not affected by p-chloromercuribenzoate (0.2 mM), EDTA (10 mM), or dithiothreitol (10 mM)...
  60. ncbi Characterization of phospholipase A2 and acyltransferase activities in squid (Loligo pealei) axoplasm: comparison with enzyme activities in other neural tissues, axolemma and axoplasmic subfractions
    M Alberghina
    Institute of Biochemistry, Faculty of Medicine, University of Catania, Italy
    Neurochem Int 21:563-71. 1992
    ..Acyltransferase activity was present at high content in both axolemma membrane rich fractions, and among subaxoplasmic fractions and axoplasmic vesicles...
  61. ncbi Reversible inhibition of NADPH-cytochrome P450 reductase by alpha-lipoic acid
    I A Slepneva
    Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia
    Biochem Biophys Res Commun 214:1246-53. 1995
    ..The results have shown that well known therapeutic agent alpha-lipoic acid is an efficient inhibitor of both purified and microsomal reductase...
  62. ncbi A novel NADPH:diamide oxidoreductase activity in arabidopsis thaliana P1 zeta-crystallin
    J Mano
    Research Institute for Food Science, Kyoto University, Uji, Japan
    Eur J Biochem 267:3661-71. 2000
    ..The diamide-reducing activity of P1-ZCr accounts for the tolerance of P1-overexpressing yeast to diamide. Other possible physiological functions of P1-ZCr in plants are discussed...
  63. ncbi Purification and characterization of a prolidase from Aureobacterium esteraromaticum
    M Fujii
    Central Laboratory, Asahi Chemical Industry Co Ltd, Shizuoka, Japan
    Biosci Biotechnol Biochem 60:1118-22. 1996
    ..Addition of Mn2+ ion did not stimulate activity. These results suggest either that the metal ion in the enzyme may be tightly bound to the polypeptide chain, or that the enzyme is not a metallo-enzyme but a thiol-enzyme...
  64. ncbi Characterization of the norovirus 3C-like protease
    Yuichi Someya
    Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Virus Res 110:91-7. 2005
    ....
  65. ncbi Protection of hexaprenyl-diphosphate synthase of Micrococcus luteus B-P 26 against inactivation by sulphydryl reagents and arginine-specific reagents
    I Yoshida
    Chemical Research Institute of Non Aqueous Solutions, Tohoku University, Sendai, Japan
    Biochim Biophys Acta 995:138-43. 1989
    ..These results suggest that the catalytic site of the synthase is formed by cooperative interaction between components A and B, and that cysteine and arginine residues on component B play important roles in the synthase activity...
  66. ncbi Vanadate changes Ca2+ influx pathway properties in human red blood cells
    L Varecka
    Department of Biochemistry and Microbiology, Slovak University of Technology, Bratislava, Slovakia
    Gen Physiol Biophys 16:359-72. 1997
    ..These results suggest that the change in the Ca2+ influx pathway properties induced by vanadate may involve changes in the metabolism of phosphoinositides but not of the arachidonate metabolism nor G-protein activation...
  67. doi Esterase patterns in species of the triatome bug Rhodnius
    E Tartarotti
    Departamento de Biologia, Instituto de Biociencias, Letras e Ciencias Exatas, Universidade Estadual Paulista, UNESP, Sao Paulo, Brazil
    Med Vet Entomol 24:26-31. 2010
    ..domesticus and R. robustus when compared with R. neivai. The present study expands our knowledge about genetic variability among triatomines and accords with the hypothesis that R. domesticus is a species derived from R. robustus...
  68. pmc Effects of thiols and mercurials on the periplasmic hydrogenase from Desulfovibrio vulgaris (Hildenborough)
    T F Fagan
    Department of Biochemistry, University College, Dublin, Ireland
    Biochem J 293:237-41. 1993
    ..This reactivation was followed by a slower irreversible inactivation, as also occurred with native enzyme; the inactivation was O2 dependent and it was partly prevented by catalase, suggesting that H2O2 may be involved...
  69. ncbi Purification and properties of an acid endo-1,4-beta-glucanase from Bacillus sp. KSM-330
    K Ozaki
    Tochigi Research Laboratories, Kao Corporation, Japan
    J Gen Microbiol 137:41-8. 1991
    ....
  70. ncbi Purification to homogeneity of latent and active 58-kilodalton forms of human neutrophil collagenase
    K A Mookhtiar
    Department of Chemistry, Florida State University, Tallahassee 32306
    Biochemistry 29:10620-7. 1990
    ..The mobility of latent HNC is consistent with a molecular weight of approximately 58K, with the active form exhibiting a slightly lower (less than 1-2K) molecular weight...
  71. ncbi The inactivation of ubiquitin accounts for the inability to demonstrate ATP, ubiquitin-dependent proteolysis in liver extracts
    A L Haas
    J Biol Chem 260:4694-703. 1985
    ..Thus the previous inability to demonstrate energy-dependent proteolysis in liver extracts is accounted for by the artifactual inactivation of ubiquitin...
  72. ncbi Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori)
    C Hirayama
    National Institute of Sericultural and Entomological Science, Ibaraki, Japan
    Insect Biochem Mol Biol 28:473-82. 1998
    ..These result show that NADH-GOGAT in the silkworm fat body strongly resembles other eukaryotic NADH-GOGATs, suggesting that it plays a significant role in ammonia assimilation in the same manner as the other enzymes...
  73. ncbi EFG-independent translocation of the mRNA:tRNA complex is promoted by modification of the ribosome with thiol-specific reagents
    Daniel R Southworth
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, MD 21205, USA
    J Mol Biol 324:611-23. 2002
    ..These data suggest that molecular targets (ribosomal proteins) in the interface region of the ribosome are critical barriers that influence the translocation of the mRNA:tRNA complex...
  74. ncbi Glutamate uptake inhibition by oxygen free radicals in rat cortical astrocytes
    A Volterra
    Center of Neuropharmacology, Institute of Pharmacological Sciences, University of Milan, Italy
    J Neurosci 14:2924-32. 1994
    ..Na+/K+ ATPase is a known target of oxygen radicals and may be involved in glutamate uptake inhibition. Indeed, ouabain, a blocker of the pump, reduces uptake in astrocytes.(ABSTRACT TRUNCATED AT 250 WORDS)..
  75. ncbi Purification and characterization of L-phenylalanine aminopeptidase from chick-pea cotyledons (Cicer arietinum L.)
    Margarita Marinova
    Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad G Bonchev Str 9, 1113 Sofia, Bulgaria
    Protein Pept Lett 16:207-12. 2009
    ..The enzyme was strongly inhibited by p-chloromercuribenzoic acid as well as in a competitive rate by the antihypertensive peptides Ile-Pro-Pro and Val-Pro-Pro...
  76. ncbi Calcium regulation by lens plasma membrane vesicles
    A Galvan
    Department of Veterinary Biology, University of Minnesota, St Paul 55108
    Arch Biochem Biophys 264:472-81. 1988
    ..The sensitivity of both calcium uptake and release to -SH reagents has implications in lens cataract formation, where oxidation of lens proteins has been proposed to account for the elevated cytosolic Ca2+ in this condition...
  77. ncbi Purification and characterization of esterases D-1 and D-2 from human erythrocytes
    Y Okada
    Department of Biochemistry, Yamanashi Medical College, Japan
    Arch Biochem Biophys 263:130-6. 1988
    ..Esterase D-2 cross-reacted with a rabbit antibody raised against the purified esterase D-1...
  78. ncbi Ribonuclease and ribonuclease inhibitor in the human pancreas
    H Kiyohara
    Gastroenterol Jpn 18:468-73. 1983
    ..RNase inhibitor is most likely a protein, which binds with both neutral and alkaline RNases. The presence of RNase inhibitor has not yet been demonstrated in the normal human serum...
  79. ncbi Characterization of an aminopeptidase and a proline iminopeptidase from cabbage leaves
    Margarita Marinova
    Institute of Organic Chemistry with Centre of Phytochemistry, Acad G Bonchev Str 9, 1113 Sofia, Bulgaria
    Z Naturforsch C 63:105-12. 2008
    ..Preliminary data of their amino acid sequences showed similarities to those of aminopeptidases N (family M1) and proline iminopeptidases (family S33)...
  80. ncbi The mechanism of contraction by 2-chloroadenosine in cat detrusor muscle cells
    S J Yang
    Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul, Republic of Korea
    J Urol 163:652-8. 2000
    ....
  81. ncbi Regulation of aspartate carbamoyltransferase in Neisseria and Branhamella species
    S Jyssum
    Kaptein W Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway
    APMIS 100:48-56. 1992
    ..An estimation of the molecular weight (Mr) of the ACTase enzyme from N. meningitidis showed it to be about 295,000, which resembles the class B enzymes found in the Enterobacteriaceae...
  82. ncbi Purification and characterization of phosphotriesterases from Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL11
    Subhas Das
    Department of Zoology, University of Delhi, India
    Can J Microbiol 52:157-68. 2006
    ..4 h at 50 degrees C), while both showed the same temperature optimum of 37 degrees C. Inhibitors like dithiothreitol and EDTA inhibited the purified enzyme, while p-chloromercuribenzoic acid and indoleacetic acid had a very little effect...
  83. pmc Denaturation studies of active-site labeled papain using electron paramagnetic resonance and fluorescence spectroscopy
    Z A Ping
    Department of Chemistry, University of Kentucky, Lexington 40506 0055
    Biophys J 60:623-8. 1991
    ..These results strongly suggest that SL-PMB and Acrylodan labels can be used to characterize the physical state of the active site of the enzyme...
  84. ncbi Purification and characterization of rat liver naloxone reductase that is identical to 3alpha-hydroxysteroid dehydrogenase
    S Yamano
    Faculty of Pharmaceutical Sciences, Fukuoka University, Japan
    Xenobiotica 29:917-30. 1999
    ..4. Amino acid sequences of five peptides obtained by proteolytic digestion of the purified enzyme were completely identical to the corresponding regions of previously reported 3alpha-hydroxysteroid dehydrogenase...
  85. doi Comparative NMR studies of diffusional water permeability of red blood cells from different species: XV. Agile wallaby (Macropus agilis), red-necked wallaby (Macropus rufogriseus) and Goodfellow's tree kangaroo (Dendrolagus goodfellowi)
    Gheorghe Benga
    Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy Cluj Napoca, Romania
    Comp Biochem Physiol A Mol Integr Physiol 154:105-9. 2009
    ..The values of E(a,d) increased after exposure of agile wallaby RBCs to PCMB, reaching a value of approximately 43-46 kJ mol(-1) when the maximal inhibition of P(d) was achieved...
  86. ncbi Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo
    Masaki Fujishima
    Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, 582 Bunkyodai, Midorimachi, Ebetsu, Hokkaido 069 8501, Japan
    New Phytol 165:513-24. 2005
    ..The enzyme tentatively classified as fructan: fructan 6(G)-fructosyltransferase (6G-FFT). The enzyme is proposed to play an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulbs...
  87. ncbi Dissociation of bovine cytochrome c1 subcomplex and the status of cysteine residues in the subunits
    K Mukai
    J Biochem 98:1417-25. 1985
    ..The conformation of the hinge protein was predicted to be composed largely of either two-alpha-helical or four-alpha-helical conformation with the amino (N)-terminal 20 residues being in a random structure...
  88. ncbi Purification and characterization of a highly thermostable chitinase from the stomach of the red scorpionfish Scorpaena scrofa with bioinsecticidal activity toward cowpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae)
    Hassiba Laribi-Habchi
    Laboratory of Environmental Biotechnology and Process Engineering LEBPE, Department of Environmental Engineering, Ecole Nationale Polytechnique ENP, avenue Pasteur, Hacene Badi, PO Box 182, El Harrach, 16200 Algiers, Algeria
    Biosci Biotechnol Biochem 76:1733-40. 2012
    ..The enzyme showed bioinsecticidal activity toward Callosobruchus maculatus, indicating the possibility of using it in biotechnological strategies for insect management for stored cowpea seeds...
  89. ncbi A new unstable, high oxygen affinity hemoglobin: Hb Nagoya or beta 97 (FG4) His----Pro
    Y Ohba
    Hemoglobin 9:11-24. 1985
    ..Subunit dissociation seemed to be slightly enhanced. The variant was markedly susceptible to oxidation and rapidly lost heme upon oxidation...
  90. ncbi Purification of an antifungal endochitinase from a potential biocontrol Agent Streptomyces griseus
    M Rabeeth
    Chikkanna Government Arts College, Tiruppur 602, Tamil Nadu, India
    Pak J Biol Sci 14:788-97. 2011
    ..griseus producing endochitinase made them suitable for biotechnological purpose such as for degradation of chitin containing waste and it might be a promising biocontrol agent for plant pathogens...
  91. pmc Purification and characterization of a prolyl aminopeptidase from Debaryomyces hansenii
    Tomás Bolumar
    Instituto de Agroquimica y Tecnologia de Alimentos, Consejo Superior de Investigaciones Cientificas, 46100 Burjassot, Valencia, Spain
    Appl Environ Microbiol 69:227-32. 2003
    ..This is the first report on the identification and purification of this type of aminopeptidase in yeast, which may contribute to the scarce knowledge about D. hansenii proteases and their possible roles in meat fermentation...
  92. ncbi Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa)
    Kazuyuki Hiwatashi
    Department of Bioengineering, Akita Research Institute of Food and Brewing, Akita, Japan
    Biosci Biotechnol Biochem 68:1395-7. 2004
    ..The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylmethylsulfonyl fluoride and arphamenin A...
  93. ncbi Accessibility of the N-ethylmaleimide-unreactive sulfhydryl of human erythrocyte Band 3
    P K Werner
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biochim Biophys Acta 982:309-15. 1989
    ....
  94. ncbi Inactivation of glyceraldehyde-3-phosphate dehydrogenase with SH-reagents and its relationship to the protein quaternary structure
    O P Malhotra
    Department of Chemistry, Banaras Hindu University, Varanasi
    Indian J Biochem Biophys 30:264-9. 1993
    ..A comparison of these data with literature reports on this and other GPDHs reveals that all reagents having large non-polar moieties exhibit half site reactivity with this enzyme...
  95. ncbi Comparative characterization of receptor and non-receptor associated protein tyrosine kinases
    A K Srivastava
    Laboratory of Diabetes and Carbohydrate Metabolism, Clinical Research Institute of Montreal, Canada
    Biochim Biophys Acta 996:13-8. 1989
    ..These data indicate that only a few but significant differences exist in the characteristics of receptor and non-receptor associated PTKs...
  96. pmc Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines
    Juan José R Coque
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Appl Environ Microbiol 69:5089-95. 2003
    ....
  97. ncbi An in vivo experimental system to study sugar phloem unloading in ripening grape berries during water deficiency stress
    Zhen ping Wang
    AGRO Montpellier, UMR 1083 Sciences pour l Oenologie et la Viticulture, 2 place P Viala, F 34060 Montpellier Cedex 1, France
    Ann Bot 92:523-8. 2003
    ..The results showed that water deficiency inhibits sugar unloading in grape berries...
  98. ncbi Retention of radiolead by human erythrocytes in vitro
    J C Barton
    Veterans Administration Medical Center, Birmingham, Alabama
    Toxicol Appl Pharmacol 99:314-22. 1989
    ..Iron and ceruloplasmin significantly decreased radiolead uptake, but inorganic metal cations other than iron, hyperosmolarity, human serum albumin, cholesterol, and transferrin had no significant effect on uptake or release...
  99. ncbi Characterization of cysteine proteases functioning in degradation of dynorphin in neuroblastoma cells: evidence for the presence of a novel enzyme with strict specificity toward paired basic residues
    M Satoh
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    J Neurochem 52:61-8. 1989
    ..Cysteine protease I was further found to show strict specificity toward the Arg-Arg doublet, when susceptibilities of various peptides containing paired basic residues were examined as substrates for the enzyme...
  100. ncbi Identification of the iron entry channels in apoferritin. Chemical modification and spectroscopic studies
    S Stefanini
    Department of Biochemical Sciences, CNR Center of Molecular Biology, University of Rome, La Sapienza, Italy
    Biochemistry 28:378-82. 1989
    ....
  101. pmc Phorbol 12-myristate 13-acetate activates an electrogenic H(+)-conducting pathway in the membrane of neutrophils
    A Kapus
    Department of Physiology, Semmelweis University Medical School, Budapest, Hungary
    Biochem J 281:697-701. 1992
    ..The activation of this route by phorbol esters seems to be independent of the stimulation of NADPH oxidase...