p chloromercuribenzoic acid

Summary

Summary: An organic mercurial used as a sulfhydryl reagent.

Top Publications

  1. ncbi Purification and some properties of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1
    Shinji Mitsuiki
    Department of Industrial Chemistry, Kyushu Sangyo University, Fukuoka, Japan
    Biosci Biotechnol Biochem 66:164-7. 2002
  2. ncbi Sensitivity of lysosomal enzymes to the plant alkaloid sanguinarine: comparison with other SH-specific agents
    T Belyaeva
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Cell Biol Int 27:887-95. 2003
  3. ncbi Diffusion pathways to critical cysteines in the vesicular acetylcholine transporter of Torpedo
    James E Keller
    Department of Chemistry and Biochemistry, The Neuroscience Research Institute, University of California, Santa Barbara, California 93106, USA
    Neurochem Res 28:477-82. 2003
  4. ncbi Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biochem 132:967-73. 2002
  5. ncbi EFG-independent translocation of the mRNA:tRNA complex is promoted by modification of the ribosome with thiol-specific reagents
    Daniel R Southworth
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, MD 21205, USA
    J Mol Biol 324:611-23. 2002
  6. ncbi The reaction of iodine and thiol-blocking reagents with human complement components C2 and factor B. Purification and N-terminal amino acid sequence of a peptide from C2a containing a free thiol group
    C Parkes
    Biochem J 213:201-9. 1983
  7. ncbi Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange
    Judith Lahav
    Coagulation Laboratory, Institute of Hematology, Rabin Medical Center Beilinson Campus, Petah Tiqva, Israel
    Blood 100:2472-8. 2002
  8. ncbi Na self inhibition of human epithelial Na channel: temperature dependence and effect of extracellular proteases
    Ahmed Chraibi
    Institut de Pharmacologie et de Toxicologie, CH 1005 Lausanne, Switzerland
    J Gen Physiol 120:133-45. 2002
  9. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
  10. ncbi Repacking of the transmembrane domains of P-glycoprotein during the transport ATPase cycle
    M F Rosenberg
    Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, UK
    EMBO J 20:5615-25. 2001

Scientific Experts

Detail Information

Publications128 found, 100 shown here

  1. ncbi Purification and some properties of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1
    Shinji Mitsuiki
    Department of Industrial Chemistry, Kyushu Sangyo University, Fukuoka, Japan
    Biosci Biotechnol Biochem 66:164-7. 2002
    ..Strain TOA-1 produced a variety of alkaline hydrolytic enzymes. An alkaline protease, designated NAPase, was purified and characterized. NAPase had a very high keratinolytic activity and high stability under acidic conditions...
  2. ncbi Sensitivity of lysosomal enzymes to the plant alkaloid sanguinarine: comparison with other SH-specific agents
    T Belyaeva
    Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia
    Cell Biol Int 27:887-95. 2003
    ..Thus, both the experimental observations obtained in this study and the literature data imply a significant role of redox-based mechanisms in regulating lysosomal functional activity...
  3. ncbi Diffusion pathways to critical cysteines in the vesicular acetylcholine transporter of Torpedo
    James E Keller
    Department of Chemistry and Biochemistry, The Neuroscience Research Institute, University of California, Santa Barbara, California 93106, USA
    Neurochem Res 28:477-82. 2003
    ..The results suggest that hydrophobic and electrostatic barriers inhibit modification of Torpedo VAChT by negatively charged organomercurials and glutathione cannot reach cysteine 2 from either side of the membrane...
  4. ncbi Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biochem 132:967-73. 2002
    ..Chemical modification studies of the CPY-I(C) complex with specific reagents demonstrated that the catalytic Ser146 and S1 substrate-binding site of CPY are covered in the complex...
  5. ncbi EFG-independent translocation of the mRNA:tRNA complex is promoted by modification of the ribosome with thiol-specific reagents
    Daniel R Southworth
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, MD 21205, USA
    J Mol Biol 324:611-23. 2002
    ..These data suggest that molecular targets (ribosomal proteins) in the interface region of the ribosome are critical barriers that influence the translocation of the mRNA:tRNA complex...
  6. ncbi The reaction of iodine and thiol-blocking reagents with human complement components C2 and factor B. Purification and N-terminal amino acid sequence of a peptide from C2a containing a free thiol group
    C Parkes
    Biochem J 213:201-9. 1983
    ..Automated Edman degradation of the peptide showed that it was the N-terminal peptide of C2a. The free thiol group was identified at position 18...
  7. ncbi Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange
    Judith Lahav
    Coagulation Laboratory, Institute of Hematology, Rabin Medical Center Beilinson Campus, Petah Tiqva, Israel
    Blood 100:2472-8. 2002
    ....
  8. ncbi Na self inhibition of human epithelial Na channel: temperature dependence and effect of extracellular proteases
    Ahmed Chraibi
    Institut de Pharmacologie et de Toxicologie, CH 1005 Lausanne, Switzerland
    J Gen Physiol 120:133-45. 2002
    ..The extracellular Na(+)-dependent inactivation has a large energy of activation and can be abolished by treatment with extracellular proteases...
  9. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
    ..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
  10. ncbi Repacking of the transmembrane domains of P-glycoprotein during the transport ATPase cycle
    M F Rosenberg
    Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, UK
    EMBO J 20:5615-25. 2001
    ..Biochemical data suggest that these rearrangements may involve rotation of transmembrane alpha-helices. A mechanism for transport is suggested...
  11. ncbi [Effect of p-chloromercuribenzoic acid and dithiothreitol on the Ca(2+) content of salivary glands and their protein secretion]
    N V Fedirko
    Ivan Franko Nataional University of Lviv
    Fiziol Zh 47:35-41. 2001
    ..Finally, we confirm important functional role of SH-groups in the regulation of Ca(2+)-homeostasis in secretory cells of exocrine glands...
  12. ncbi Isolation and characterization of mitochondrial F(1)-ATPase from crayfish (Orconectes virilis) gills
    Z Li
    Mount Sinai School of Medicine, Department of Human Genetics, 1425 Madison Ave, New York, NY 10029, USA
    Comp Biochem Physiol B Biochem Mol Biol 128:325-38. 2001
    ..In addition, an unmodified F(0) stabilizes F(1) and decreases F(1) sensitivities to inhibitors and modulators...
  13. ncbi Partial purification and kinetic properties of three different D-glucosamine 6-P:N-acetyltransferase forms from human placenta
    M Vessal
    Department of Biochemistry, Shiraz University of Medical Sciences, Iran
    Comp Biochem Physiol B Biochem Mol Biol 121:379-84. 1998
    ..The results are discussed in terms of differences among the enzyme properties of human placental, rodent and porcine liver forms...
  14. ncbi Identification of residues in the translocation pathway of EmrE, a multidrug antiporter from Escherichia coli
    M Lebendiker
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 271:21193-9. 1996
    ..The results demonstrate the existence of a domain accessible only to substrates and provide a unique tool for studying the substrate permeation pathway of an ion-coupled transporter...
  15. ncbi Purification and characterization of a novel carbaryl hydrolase from Aspergillus niger PY168
    Qing Zhang
    The Key Laboratory of Gene Engineering of Ministry of Education, Sun Yet-sen University, Guangzhou 510275, PR China
    FEMS Microbiol Lett 228:39-44. 2003
    ..The purified enzyme hydrolyzed various N-methylcarbamate insecticides. Carbaryl is the preferred substrate...
  16. ncbi Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus
    Hosam E Ewis
    Department of Biology, Georgia State University, 24 Peachtree Center Avenue, Atlanta, GA 30303, USA
    Gene 329:187-95. 2004
    ..The enzymatic properties of Est30 and Est55 reported here warrant the potential applications of these enzymes in biotechnological industries...
  17. ncbi The role of monocarboxylate transporter 2 and 4 in the transport of gamma-hydroxybutyric acid in mammalian cells
    Qi Wang
    Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, State University of New York, Amherst, NY 14260, USA
    Drug Metab Dispos 35:1393-9. 2007
    ..This investigation demonstrated that GHB is a substrate for both MCT2 and MCT4; these transporters may be important in the nonlinear disposition of GHB, as well as influencing its tissue distribution...
  18. ncbi The role of monocarboxylate transporters in uptake of lactic acid in HeLa cells
    Sravanthi Cheeti
    Division of Pharmaceutical Sciences, University of Missouri, School of Pharmacy, 5005 Rock Hill Road, Kansas City, MO 64110-2499, USA
    Int J Pharm 325:48-54. 2006
    ..MCT isoforms 1, 5 and 6 in HeLa cells were identified by RT-PCR. HeLa cell line can be used as an effective screening tool for intravaginally administered drugs targeted toward MCT...
  19. ncbi Monocarboxylate transporter 1 (MCT1) mediates transport of short-chain fatty acids in bovine caecum
    Doaa Kirat
    Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069 8501, Japan
    Exp Physiol 91:835-44. 2006
    ..The results show that MCT1 is a major route for SCFA efflux across the basolateral membrane of bovine large intestine and that it could play a role in the regulation of intracellular pH...
  20. ncbi Functional activity of a monocarboxylate transporter, MCT1, in the human retinal pigmented epithelium cell line, ARPE-19
    Soumyajit Majumdar
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri Kansas City, 5005 Rockhill Road, Kansas City, MO 64110, USA
    Mol Pharm 2:109-17. 2005
    ..This cell line may thus be used as an effective screening tool for intravitreally administered drugs/prodrugs targeted toward MCT1 expressed on the RPE...
  21. ncbi Simultaneous modulation of transport and metabolism of acyclovir prodrugs across rabbit cornea: An approach involving enzyme inhibitors
    Suresh Katragadda
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City, 5005 Rockhill Road, Kansas City, MO 64110-2499, USA
    Int J Pharm 320:104-13. 2006
    ..In conclusion, utility of enzyme inhibitors to modulate transport and metabolism of prodrugs appears to be promising strategy for enhancing drug transport across cornea...
  22. ncbi Purification and properties of an acid endo-1,4-beta-glucanase from Bacillus sp. KSM-330
    K Ozaki
    Tochigi Research Laboratories, Kao Corporation, Japan
    J Gen Microbiol 137:41-8. 1991
    ....
  23. ncbi Critical role of the cysteine 323 residue in the catalytic activity of human glutamate dehydrogenase isozymes
    Seung Ju Yang
    Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138 736, Korea
    Mol Cells 19:97-103. 2005
    ..There were no differences between the two hGDH isozymes in sensitivities to mutagenesis at C323. Our results suggest that C323 plays an important role in catalysis by human GDH isozymes...
  24. ncbi The intracellular pathway of the acetylcholine-induced contraction in cat detrusor muscle cells
    J Y An
    Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul, 156-756, Republic of Korea
    Br J Pharmacol 137:1001-10. 2002
    ..5. These results suggest that in cat detrusor muscle contraction induced by ACh is mediated via M3 muscarinic receptor-dependent activation of Gq/11 and PLC-beta1 and IP3-dependent Ca(2+) release...
  25. ncbi The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolution
    I P Korndörfer
    Institute of Molecular Biology Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, OR, 97403 1229, USA
    J Mol Biol 300:917-33. 2000
    ..The available structural data suggest that a metal-mediated hydride-shift mechanism, which is generally favored for xylose isomerase, is also feasible for rhamnose isomerase...
  26. ncbi Inhibition of l-arginine transport in platelets by asymmetric dimethylarginine and N-monomethyl-l-arginine: effects of arterial hypertension
    Tmc Brunini
    Departamento de Farmacologia e Psicobiologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil
    Clin Exp Pharmacol Physiol 31:738-40. 2004
    ..It is possible that endogenous l-arginine analogues, by inhibiting NO synthesis, are involved in the platelet activation present in hypertension...
  27. ncbi [Effect of inhibitors of energy-dependent Ca2+-transporting systems on ATP-hydrolase activity of smooth muscle actomyosin]
    S A Kosterin
    Palladine Institute of Biochemistry, NAS of Ukraine, Kyiv
    Ukr Biokhim Zh 73:57-62. 2001
    ..The data obtained could be used for the further development of the ideas about regularities of Ca(2+)-dependent control of the smooth muscles contraction-relaxation...
  28. ncbi Purification and characterization of a recombinant listeriolysin O expressed in Escherichia coli and possible diagnostic applications
    Camilla Giammarini
    Centro di Biotecnologie, , Via T. Campanella 1, 61032 Fano, Italy
    J Biotechnol 109:13-20. 2004
    ..Here we report the characterization of toxin properties and its preliminary application in an ELISA diagnostic test for listeriosis...
  29. ncbi Biochemical characterization of human eosinophil Charcot-Leyden crystal protein (lysophospholipase)
    P F Weller
    J Biol Chem 259:15100-5. 1984
    ..1%, sodium dodecyl sulfate. The hydrophobic nature of this protein, which accounts for 10% of the protein of the eosinophil, may contribute to its unique propensity for crystallization in vivo...
  30. ncbi X-ray diffraction of heavy-atom labelled two-dimensional crystals of rhodopsin identifies the position of cysteine 140 in helix 3 and cysteine 316 in helix 8
    Thorsten Mielke
    , Biophysics Group, Department of Physics, Berlin, Germany
    J Mol Biol 316:693-709. 2002
    ....
  31. ncbi Role of cysteine residues in glutathione synthetase from Escherichia coli B. Chemical modification and oligonucleotide site-directed mutagenesis
    H Kato
    Institute for Chemical Research, Kyoto University, Japan
    J Biol Chem 263:11646-51. 1988
    ..Replacement of Cys-122 to Ala-122 enhanced the reactivity of Cys-289 with sulfhydryl reagents...
  32. ncbi Existence of an iron-oxidizing bacterium Acidithiobacillus ferrooxidans resistant to organomercurial compounds
    Fumiaki Takeuchi
    Environmental Management and Safety Section, Health and Environment Center, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan
    J Biosci Bioeng 99:586-91. 2005
    ..The cytosol prepared from MON-1 could volatilize Hg0 from PCMB (0.015 nmol mg(-1) h(-1)), PMA (0.33 nmol mg(-1) h(-1)) and MMC (0.005 nmol mg(-1) h(-1)) in the presence of NADPH and beta-mercaptoethanol...
  33. ncbi The mechanism of contraction by 2-chloroadenosine in cat detrusor muscle cells
    S J Yang
    Department of Pharmacology, College of Pharmacy, Chung Ang University, Seoul, Republic of Korea
    J Urol 163:652-8. 2000
    ....
  34. ncbi Extracellular alpha-amylase from Thermus filiformis Ork A2: purification and biochemical characterization
    M C Egas
    Departamento de Bioquimica, Universidade de Coimbra, Portugal
    Extremophiles 2:23-32. 1998
    ..To our knowledge this is the first report of the characterization of an alpha-amylase from a strain of the genus Thermus...
  35. ncbi Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori)
    C Hirayama
    National Institute of Sericultural and Entomological Science, Ibaraki, Japan
    Insect Biochem Mol Biol 28:473-82. 1998
    ..These result show that NADH-GOGAT in the silkworm fat body strongly resembles other eukaryotic NADH-GOGATs, suggesting that it plays a significant role in ammonia assimilation in the same manner as the other enzymes...
  36. ncbi A novel alpha-glucosidase from the moss Scopelophila cataractae
    Yoshiki Yamasaki
    Research Institute for Bioresources, Okayama University, Okayama, Japan
    Acta Biochim Pol 54:401-6. 2007
    ..These results show that alpha-glucosidases should be capable of complete starch digestion by themselves in cells of S. cataractae...
  37. ncbi A novel NADPH:diamide oxidoreductase activity in arabidopsis thaliana P1 zeta-crystallin
    J Mano
    Research Institute for Food Science, Kyoto University, Uji, Japan
    Eur J Biochem 267:3661-71. 2000
    ..The diamide-reducing activity of P1-ZCr accounts for the tolerance of P1-overexpressing yeast to diamide. Other possible physiological functions of P1-ZCr in plants are discussed...
  38. ncbi Purification and some properties of two enzymes from rat liver cytosol that catalyze carbonyl reduction of 6-tert-butyl-2, 3-epoxy-5-cyclohexene-1,4-dione, a metabolite of 3-tert-butyl-4-hydroxyanisole
    K Tajima
    Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, 920 1181, Japan
    Arch Biochem Biophys 361:207-14. 1999
    ..Dicoumarol inhibited significantly TBE-R1 activity but not TBE-R2 activity. In the conversion of TBE to TBEH, TBE-R1 preferentially reduced TBE to (4R)-TBEH, whereas TBE-R2 preferred the reduction of TBE to (4S)-TBEH...
  39. ncbi Volatilization and recovery of mercury from mercury-polluted soils and wastewaters using mercury-resistant Acidithiobacillus ferrooxidans strains SUG 2-2 and MON-1
    Fumiaki Takeuchi
    Environmental Management and Safety Section, Health and Environment Center, Okayama University, 3 1 1, Tsushima Naka, Okayama 700 8530, Japan
    Environ Sci 13:305-16. 2006
    ..5) containing 40 microM Hg(2+) and ferrous iron. The MON-1-immobilized PVA resins were used repeatedly...
  40. ncbi Differential metabolism of Tyr-MIF-1 and MIF-1 in rat and human plasma
    A J Kastin
    VA Medical Center, New Orleans, LA
    Biochem Pharmacol 47:699-709. 1994
    ....
  41. ncbi Identification of valproic acid glucuronide hydrolase as a key enzyme for the interaction of valproic acid with carbapenem antibiotics
    Eiko Suzuki
    Drug Metabolism and Pharmacokinetics Research Laboratories, Daiichi Sankyo Co, Ltd, 1 2 58 Hiromachi, Shinagawa ku, Tokyo, Japan
    Drug Metab Dispos 38:1538-44. 2010
    ..Taken together, APEH plays a major role in the PAPM-sensitive VPA-G hydrolysis in the liver. These findings suggest that APEH could be a key enzyme for the drug interaction of VPA with carbapenems via VPA-G hydrolysis...
  42. ncbi Very long chain and long chain acyl-CoA thioesterases in rat liver mitochondria. Identification, purification, characterization, and induction by peroxisome proliferators
    L T Svensson
    Department of Metabolic Research, Wenner Gren Institute Arrhenius Laboratories F3, Stockholm University, Sweden
    J Biol Chem 270:12177-83. 1995
    ..Comparison of physical and catalytical characteristics of the enzymes studied here and previously purified acyl-CoA thioesterases suggest that MTE-I and MTE-II are novel enzymes...
  43. ncbi Characterization of an alkaline protease from Bacillus sp. no. AH-101
    H Takami
    Department of Microbial Ecology, Institute of Physical and Chemical Research, Saitama, Japan
    Appl Microbiol Biotechnol 33:519-23. 1990
    ..The no. AH-101 alkaline protease was compared with subtilisin BPN', subtilisin Carlsberg, no. 221, and Ya-B alkaline proteases. Extensive sequence homology existed among these enzymes...
  44. ncbi The multiple site binding of carboxypeptidase Y inhibitor (IC) to the cognate proteinase. Implications for the biological roles of the phosphatidylethanolamine-binding protein
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biol Chem 278:29792-8. 2003
    ..Considering the fact that mouse PEBP has recently been identified as a novel thrombin inhibitor, the binding that characterizes the CPY-IC complex could be a common feature of PEBPs...
  45. ncbi [Involvement of SH-groups in the regulation of adenylate cyclase signal system by isoproterenol and serotonin in cultured murine fibroblasts L (subline LSM)]
    A O Shpakov
    Institute of Evolutionary Physiology and Biochemistry RAS, St Petersburg
    Tsitologiia 43:1130-5. 2001
    ..The forms of SH-groups in proteins involved in the ACS determine their functional activities and a possibility of transduction of the hormonal signal on the effector systems...
  46. ncbi Purification and characterization of chitinase from Streptomyces sp. M-20
    Kyoung Ja Kim
    Department of Life Science, Soonchunhyang University, Asan si 336 745, Korea
    J Biochem Mol Biol 36:185-9. 2003
    ..The chitinase activity was high on colloidal chitin, chitotriose, and chitooligosaccharide. The purified chitinase showed antifungal activity against Botrytis cinerea, and lysozyme activity against the cell wall of Botrytis cinerea...
  47. ncbi Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4-carboxylic acid
    K Mochizuki
    National Institute of Bioscience and Human Technology, AIST, 1 1, Higashi, Tsukuba, Ibaraki 305 8566, Japan
    Arch Microbiol 172:182-5. 1999
    ..The enzyme required ATP, Mg(2+), and KCl for the cleavage of L-2-oxothiazolidine-4-carboxylic acid. The enzyme also cleaved 5-oxo-L-proline to L-glutamic acid and is considered to be 5-oxo-L-prolinase...
  48. ncbi Biliverdin-IX alpha reductase and biliverdin-IX beta reductase from human liver. Purification and characterization
    T Yamaguchi
    Department of Biochemical Genetics, Tokyo Medical and Dental University, Japan
    J Biol Chem 269:24343-8. 1994
    ..2 whereas that for isozymes III and IV was 7.4. The proportion of the total activity of isozymes I and II to that of isozymes III and IV was considerably higher in the fetal than in the adult liver...
  49. ncbi cDNA cloning of MCT2, a second monocarboxylate transporter expressed in different cells than MCT1
    C K Garcia
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235 9046
    J Biol Chem 270:1843-9. 1995
    ..These findings suggest that MCT1 and MCT2 are adapted to play different roles in monocarboxylate transport in different cells of the body...
  50. ncbi Oxygen consumption rates and oxygen concentration in molt-4 cells and their mtDNA depleted (rho0) mutants
    Jiangang Shen
    EPR Center, Department of Diagnostic Radiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Biophys J 84:1291-8. 2003
    ..The results are consistent with our earlier findings suggesting that such oxygen gradients may be a general phenomenon found in most or all cell systems under appropriate conditions...
  51. ncbi Human brain contains a novel non-AT1, non-AT2 binding site for active angiotensin peptides
    Vardan T Karamyan
    Department of Pharmacology, School of Pharmacy, University of Mississippi, University, MS 38677, USA
    Life Sci 83:421-5. 2008
    ..To determine whether the novel non-AT1, non-AT2 binding site for angiotensins recently discovered in rodent brains occurs in the human brain...
  52. ncbi Regional distribution and age-dependent expression of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain
    Jun Morishita
    Department of Biochemistry, Kagawa University School of Medicine, Kagawa, Japan
    J Neurochem 94:753-62. 2005
    ..These results demonstrated wide distribution of NAPE-PLD in various brain regions and its age-dependent expression, suggesting the central role of this enzyme in the formation of anandamide and other N-acylethanolamines in the brain...
  53. ncbi Isolation and characterization of novel long-chain acyl-CoA thioesterase/carboxylesterase isoenzymes from Candida rugosa
    M A Diczfalusy
    Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, Sweden
    Arch Biochem Biophys 334:104-12. 1996
    ..N-terminal sequence analysis and labeling by DFP suggest that these long-chain acyl-CoA thioesterases belong to a novel group of yeast serine esterases...
  54. ncbi Purification and characterization of L-phenylalanine aminopeptidase from chick-pea cotyledons (Cicer arietinum L.)
    Margarita Marinova
    Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad G Bonchev Str 9, 1113 Sofia, Bulgaria
    Protein Pept Lett 16:207-12. 2009
    ..The enzyme was strongly inhibited by p-chloromercuribenzoic acid as well as in a competitive rate by the antihypertensive peptides Ile-Pro-Pro and Val-Pro-Pro...
  55. ncbi Identification of a novel non-AT1, non-AT2 angiotensin binding site in the rat brain
    Vardan T Karamyan
    Department of Pharmacology and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA
    Brain Res 1143:83-91. 2007
    ..4.24.15 and/or EC 3.4.24.16. However, some unique characteristics of this non-AT1, non-AT2 binding site suggest that it may be a novel angiotensin binding substance...
  56. ncbi Cell death and cytotoxic effects in YAC-1 lymphoma cells following exposure to various forms of mercury
    Margaret Yole
    Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, University of Saskatchewan, Saskatoon SK S7N 5B4, Canada
    Toxicology 231:40-57. 2007
    ..These findings indicate the two groups of Hg compounds may induce cell death by distinct pathways, reflecting interactions with different cellular targets leading to cell death...
  57. ncbi Esterase patterns and phylogenetic relationships of Drosophila species in the saltans subgroup (saltans group)
    A P Nascimento
    , Letras e Ciencias Exatas-UNESP, , SP, Brazil
    Genetica 114:41-51. 2002
    ..septentriosaltans, D. saltans and D. austrosaltans are closer to each other than to D. prosaltans. These results showed to be consistent with phylogenetic relationships previously inferred from inversion polymorphism...
  58. ncbi Salt stress enhances choline uptake in the halotolerant cyanobacterium Aphanothece halophytica
    Aran Incharoensakdi
    Laboratory of Biochemical Products, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand
    Biochim Biophys Acta 1621:102-9. 2003
    ..The results of competition studies suggested that N-methyl on one end of molecule and on the other end either an aldehyde, an alcohol or a neutral group were important features for substrate recognition...
  59. ncbi 2-(2'-Hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2: purification and characterization
    J Konishi
    Bio Refining Process Laboratory, Japan Cooperation Center, Petroleum, 1900 Sodeshi cho, 424 0037, Shimizu, Shizuoka, Japan
    Appl Microbiol Biotechnol 62:356-61. 2003
    ..TdsB desulfurized 2-(2'-hydroxyphenyl)ethen-1-sulfinate to yield o-hydroxystyrene, but DszB could not. A site-directed mutagenesis study revealed the cysteine residue at position 17 to be essential to the catalytic activity of TdsB...
  60. ncbi Isolation and characterization of a cysteine protease of freesia corms
    Tetsuya Uchikoba
    Kagoshima University Musium, Japan
    Biosci Biotechnol Biochem 66:448-52. 2002
    ..From the hydrolysis of peptidyl-p-NAs, the specificity of FP-A was found to be broad. It was thought that FP-A was a new protease from freesia corms...
  61. ncbi Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism
    Li Ying Zhao
    Department of Biochemistry, Kagawa University School of Medicine, 1750 1 Ikenobe, Miki, Kagawa 761 0793, Japan
    Biochim Biophys Acta 1771:1397-405. 2007
    ..The results exhibited that Asn-37, Asn-107, Asn-309, and Asn-333 are actual N-glycosylation sites. The glycosylation appeared to play an important role in stabilizing the enzyme protein...
  62. ncbi Brain angiotensin receptors and binding proteins
    Robert C Speth
    Department of Pharmacology, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, Oxford, MS 38677, USA
    Naunyn Schmiedebergs Arch Pharmacol 377:283-93. 2008
    ..g., stimulation of dopamine release from the striatum, as a peptidase, or as a clearance receptor, and the importance of the state of the internal environment of the brain to its function is reviewed...
  63. ncbi Characterization of an aminopeptidase and a proline iminopeptidase from cabbage leaves
    Margarita Marinova
    Institute of Organic Chemistry with Centre of Phytochemistry, Acad G Bonchev Str 9, 1113 Sofia, Bulgaria
    Z Naturforsch C 63:105-12. 2008
    ..Preliminary data of their amino acid sequences showed similarities to those of aminopeptidases N (family M1) and proline iminopeptidases (family S33)...
  64. ncbi Characterization of the brain-specific non-AT(1), non-AT(2) angiotensin binding site in the mouse
    Vardan T Karamyan
    Department of Pharmacology, School of Pharmacy, University of Mississippi, University, MS 38677, United States
    Eur J Pharmacol 590:87-92. 2008
    ..Whether this binding site functions as a novel receptor for angiotensins or a specific angiotensinase with variable functionality at different redox states will require further study...
  65. ncbi Isolation and characterization of a new extracellular bacteriolytic endopeptidase of Lysobacter sp. XL1
    O A Stepnaya
    Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia
    Biochemistry (Mosc) 70:1031-7. 2005
    ..The enzyme is inhibited by p-chloromercuribenzoic acid by 72% and by phenylmethylsulfonyl fluoride by 43%, which indicates the involvement of serine and thiol groups in its functioning...
  66. ncbi Purification and characterization of a prolidase from Aureobacterium esteraromaticum
    M Fujii
    Central Laboratory, Asahi Chemical Industry Co Ltd, Shizuoka, Japan
    Biosci Biotechnol Biochem 60:1118-22. 1996
    ..Addition of Mn2+ ion did not stimulate activity. These results suggest either that the metal ion in the enzyme may be tightly bound to the polypeptide chain, or that the enzyme is not a metallo-enzyme but a thiol-enzyme...
  67. ncbi An in vivo experimental system to study sugar phloem unloading in ripening grape berries during water deficiency stress
    Zhen-Ping Wang
    AGRO Montpellier, UMR 1083 'Sciences pour l'Oenologie et la Viticulture, 2 place P.Viala, F-34060 Montpellier Cedex 1, France
    Ann Bot (Lond) 92:523-8. 2003
    ..The results showed that water deficiency inhibits sugar unloading in grape berries...
  68. ncbi Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines
    Juan José R Coque
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Appl Environ Microbiol 69:5089-95. 2003
    ....
  69. ncbi Assay of dehydrogenases with an O2-consuming biosensor
    A Haouz
    Laboratoire de Technologies des Enzymes et des Biomembranes, Universite Paris 7 Denis Diderot, France
    Enzyme Microb Technol 16:292-7. 1994
    ..NAD+ is recycled. The detection limit was 50 nM. Applications can be found in medicine, the food industry, and the environment...
  70. ncbi Comparative characterization of receptor and non-receptor associated protein tyrosine kinases
    A K Srivastava
    Laboratory of Diabetes and Carbohydrate Metabolism, Clinical Research Institute of Montreal, Canada
    Biochim Biophys Acta 996:13-8. 1989
    ..These data indicate that only a few but significant differences exist in the characteristics of receptor and non-receptor associated PTKs...
  71. ncbi Accessibility of the N-ethylmaleimide-unreactive sulfhydryl of human erythrocyte Band 3
    P K Werner
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biochim Biophys Acta 982:309-15. 1989
    ....
  72. ncbi Chlorophyllase as a serine hydrolase: identification of a putative catalytic triad
    Tohru Tsuchiya
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori ku, Yokohama, Kanagawa, 226 8501 Japan
    Plant Cell Physiol 44:96-101. 2003
    ..These results indicate that Chlase is a serine hydrolase and, by analogy with a plausible catalytic mechanism of serine hydrolases, we proposed a mechanism for hydrolysis catalyzed by Chlase...
  73. ncbi The thiol proteinases from the latex of Carica papaya L. I. Fractionation, purification and preliminary characterization
    T Dubois
    Laboratoire de Chimie Générale I, université de Bruxelles
    Biol Chem Hoppe Seyler 369:733-40. 1988
    ..The active sites of chymopapain and proteinase omega could not be distinguished from that of papain neither by the analysis of the pH dependence of kcat/Km nor by the examination of the pH dependence of the fluorescence emission spectra...
  74. ncbi Retention of radiolead by human erythrocytes in vitro
    J C Barton
    Veterans Administration Medical Center, Birmingham, Alabama
    Toxicol Appl Pharmacol 99:314-22. 1989
    ..Iron and ceruloplasmin significantly decreased radiolead uptake, but inorganic metal cations other than iron, hyperosmolarity, human serum albumin, cholesterol, and transferrin had no significant effect on uptake or release...
  75. ncbi Identification of the iron entry channels in apoferritin. Chemical modification and spectroscopic studies
    S Stefanini
    Department of Biochemical Sciences, CNR Center of Molecular Biology, University of Rome, La Sapienza, Italy
    Biochemistry 28:378-82. 1989
    ....
  76. ncbi Mechanism of inhibition of human neutrophil collagenase by Gold(I) chrysotherapeutic compounds. Interaction at a heavy metal binding site
    S K Mallya
    Department of Chemistry, Florida State University, Tallahassee 32306
    J Biol Chem 264:1594-601. 1989
    ..Occupancy of this site by Zn(II) is characterized by retention of activity...
  77. ncbi Study of biochemical pathway and enzyme involved in metsulfuron-methyl degradation by Ancylobacter sp. XJ-412-1 isolated from soil
    Peng Lu
    Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, People s Republic of China
    Curr Microbiol 62:1718-25. 2011
    ..The carboxyesterase activity of the cell-free extracts was assayed and strongly inhibited by 4-chloromercuribenzoic acid (PCMB), diethyl pyrocarbonate (DEPC), phenylmethylsulfonyl fluoride (PMSF), and malathion...
  78. ncbi Characterization of interactions involving anti-metatype antibodies and immune complexes
    K M Weidner
    Department of Microbiology, University of Illinois, Urbana 61801
    Mol Immunol 29:303-12. 1992
    ....
  79. ncbi Oxidized glutathione causes sensitization of calcium release to inositol 1,4,5-trisphosphate in permeabilized hepatocytes
    D C Renard
    Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107
    Biochem J 284:507-12. 1992
    ..The data suggest that the sensitivity of the intracellular Ca2+ pools to release by Ins(1,4,5)P3 can be modulated by the formation of mixed disulphides with GSSG or other oxidized thiols...
  80. ncbi Characterization of NADP+: 3 beta-hydroxysteroid dehydrogenase from microsomes of rat liver
    T Akao
    Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan
    Biochim Biophys Acta 1046:271-6. 1990
    ..The molecular weight of the enzyme was estimated to be about 43,000 by Sephadex G-200 column chromatography. From these results, it is concluded that the enzyme is a new type of microsomal NADP+:3 beta-hydroxysteroid dehydrogenase...
  81. ncbi 4-Bromoacetamidoprocaine: an affinity ligand for brain muscarinic and nicotinic cholinergic receptors
    M Saraswati
    Department of Pharmacology, University of Rochester Medical Center, NY 14642
    Neurochem Res 17:247-52. 1992
    ..The findings indicate that BAP is a useful affinity ligand for examining the biochemical and functional characteristics of brain cholinergic receptors, particularly the muscarinic which has an affinity near the nM concentration range...
  82. ncbi The trypsin-catalyzed activation of glutamate dehydrogenase purified from eel liver
    M Q Tang
    Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University
    J Biochem 111:655-61. 1992
    ..p-Chloromercuribenzoic acid inhibited the activity of the trypsin-treated GDH, but stimulated that of the native GDH. The response of trypsin-treated GDH to ADP and GTP was decreased compared with that of the native GDH...
  83. ncbi Superoxide anion production by lipoamide dehydrogenase redox-cycling: effect of enzyme modifiers
    L Grinblat
    Centro de Investigaciones Bioenergéticas, Facultad de Medicina, Buenos Aires, Argentina
    Biochem Int 23:83-92. 1991
    ..Comparison of heart and yeast enzyme preparations revealed a close correlation between lipoamide reductase and NADH-oxidase activities. It is concluded that O2-. production is a molecular property of lipoamide dehydrogenase...
  84. ncbi Thyrotropin-releasing hormone-degrading enzyme in human serum is classified as type II of pyroglutamyl aminopeptidase: influence of thyroid status
    M Yamada
    First Department of Internal Medicine, Gunma University, School of Medicine, Maebashi, Japan
    Proc Soc Exp Biol Med 194:346-51. 1990
    ..The present data indicate that a TRH-degrading enzyme in human serum possesses a nature identical to type II of pGlu-peptidase which is not altered by thyroid status...
  85. ncbi Cellobiose uptake by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes
    L K Maas
    Department of Microbiology, North Dakota State University, Fargo 58105
    Can J Microbiol 37:141-7. 1991
    ..Na+, but not Li+, stimulated cellobiose transport...
  86. ncbi Characteristics of thiol:protein disulfide oxidoreductase from wheat (Triticum aestivum L.) grain
    S V Osipova
    Siberian Institute of Plant Physiology and Biochemistry, Siberian Division of the Russian Academy of Sciences, Irkutsk, 664033, Russia
    Biochemistry (Mosc) 70:935-40. 2005
    ..The proposed physiological role of TPDO is participation in creation and regulation of SH/SS-status of wheat endosperm proteins and formation of the rheological properties of gluten...
  87. ncbi Activation of potassium efflux from Escherichia coli by glutathione metabolites
    M J Elmore
    Department of Genetics and Microbiology, University of Aberdeen, Marischal College, UK
    Mol Microbiol 4:405-12. 1990
    ..It is concluded that the KefB and KefC systems are gated by glutathione metabolites and that the degree to which they are activated is dependent upon the nature of the substituent on the sulphydryl group...
  88. ncbi Purification and characterization of a new arginine carboxypeptidase in human serum
    D Hendriks
    Department of Pharmaceutical Sciences, University of Antwerp, Wilrijk, Belgium
    Biochim Biophys Acta 1034:86-92. 1990
    ..These characteristics differentiate carboxypeptidase U from carboxypeptidase N and other known carboxypeptidases...
  89. ncbi The action of organic mercury compounds on the function of isolated mammalian heart muscle
    S Halbach
    Institut fur Toxikologie, Gesellschaft für Strahlen und Umweltforschung, Neuherberg, F R G
    Eur J Pharmacol 167:255-64. 1989
    ..This notion was confirmed by experiments with isolated membrane particles. The prevalence of the negative or positive inotropic action of these compounds could be ascribed to their lipophilic or hydrophilic properties, respectively...
  90. ncbi Phorbol 12-myristate 13-acetate activates an electrogenic H(+)-conducting pathway in the membrane of neutrophils
    A Kapus
    Department of Physiology, Semmelweis University Medical School, Budapest, Hungary
    Biochem J 281:697-701. 1992
    ..The activation of this route by phorbol esters seems to be independent of the stimulation of NADPH oxidase...
  91. ncbi Production of long-chain alcohols by yeasts
    M J White
    Zymology Laboratory, School of Biological Sciences, Bath University, Avon, UK
    J Gen Microbiol 133:2181-90. 1987
    ..The fatty-acyl chain-length profile of the triacylglycerol and to a lesser extent the sterol/wax ester fractions from C. albicans reflected that of the long-chain alcohols produced by this yeast...
  92. ncbi N-terminal acetyl group is essential for the inhibitory function of carboxypeptidase Y inhibitor (I(C))
    Joji Mima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    FEBS Lett 532:207-10. 2002
    ..Thus, the N-terminal acetyl group of I(C) is essential for achieving a tight interaction with CPY and for its complete inactivation...
  93. ncbi Characterization and partial purification of microsomal NAD(P)H:quinone oxidoreductases
    A K Jaiswal
    Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA
    Arch Biochem Biophys 375:62-8. 2000
    ..The microsomal mNQO proteins are expected to provide additional protection after cytosolic NQOs against quinone toxicity and mutagenicity...
  94. ncbi Purification and characterization of human biliverdin reductase
    M D Maines
    Department of Biophysics, University of Rochester School of Medicine, New York 14642
    Arch Biochem Biophys 300:320-6. 1993
    ..Of the seven cysteine residues present in the human, four or five were titratable with 5'-dithiobis(2-nitrobenzoic acid)...
  95. ncbi A monocarboxylate transporter MCT1 is located at the basolateral pole of rat jejunum
    M N Orsenigo
    Dipartimento di Fisiologia e Biochimica Generali, Universita di Milano, Via Celoria 26, I 20133 Milano, Italy
    Exp Physiol 84:1033-42. 1999
    ....
  96. ncbi Characterization of an NADH-linked cupric reductase activity from the Escherichia coli respiratory chain
    V A Rapisarda
    Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas Consejo Nacional de Investigaciones Científicas y Tecnológicas Universidad Nacional de Tucumán, San Miguel de Turcumán, Argentina
    Arch Biochem Biophys 370:143-50. 1999
    ..To our knowledge, this is the first protein with cupric reductase activity to be isolated and characterized in its biochemical properties...
  97. ncbi Enzymic characterization in vitro of recombinant proprotein convertase PC4
    A Basak
    Molecular Neuroendocrinology Laboratory, Clinical Research Institute of Montreal, Montreal, Que, Canada
    Biochem J 343:29-37. 1999
    ..PC4A was also inhibited by transition-metal ions (Cu(2+)>Hg(2+)>Zn(2+) Ni(2+)>Co(2+)), as well as by small peptide semicarbazones (SCs), such as Arg-Lys-Lys-Arg-SC (K(i) 0.75 microM) and Arg-Ser-Lys-Arg-SC (K(i) 11.4 microM)...
  98. ncbi Analysis and modification of thiols in the hemolymph juvenile hormone binding protein of Manduca sexta
    Y C Park
    Department of Entomology, University of Wisconsin Madison 53706
    Arch Biochem Biophys 302:12-8. 1993
    ..The analyses support the observation that there is a single form of the hemolymph juvenile hormone-binding protein in our strain of M. sexta...
  99. ncbi Functional analysis of a genetic defect of copper transport (Menkes disease) in different cell lines
    Y Qian
    Department of Biochemistry, Texas A and M University, College Station 77843, USA
    Am J Physiol 271:C378-84. 1996
    ..As exemplified by BeWo and Menkes cells, failure to efflux Cu ions may be linked with the failure to express a functional Cu-transporting ATPase, namely, the Menkes protein...
  100. ncbi The amino acid transport system y+L/4F2hc is a heteromultimeric complex
    R Estevez
    Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Barcelona 08028
    FASEB J 12:1319-29. 1998
    ..To our knowledge, this is the first direct evidence for a heteromultimeric protein structure of an organic solute carrier in mammals...
  101. ncbi Coincident expression of Menkes gene with copper efflux in human placental cells
    Y Qian
    Department of Biochemistry and Biophysics Texas A and M University, College Station 77843 2128, USA
    Am J Physiol 270:C1880-4. 1996
    ..The data link the Menkes gene product with the ability of cells to release copper ions. They also suggest that the expression of the Menkes gene may be regulated by the development of polarized cell membranes...