phosphorylcholine

Summary

Summary: Calcium and magnesium salts used therapeutically in hepatobiliary dysfunction.

Top Publications

  1. ncbi Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda
    Philippe J Guerin
    Norwegian Institute of Public Health, Norway
    Lancet Infect Dis 2:494-501. 2002
  2. pmc Miltefosine induces apoptosis-like death in Leishmania donovani promastigotes
    Caroline Paris
    UMR 8076 CNRS, Chimiothérapie Antiparasitaire, France
    Antimicrob Agents Chemother 48:852-9. 2004
  3. pmc Phosphorylcholine decreases early inflammation and promotes the establishment of stable biofilm communities of nontypeable Haemophilus influenzae strain 86-028NP in a chinchilla model of otitis media
    Wenzhou Hong
    Department of Microbiology and Immunology, Wake Forest University Health Sciences, Winston Salem, NC 27157, USA
    Infect Immun 75:958-65. 2007
  4. ncbi Oral miltefosine for Indian visceral leishmaniasis
    Shyam Sundar
    Institutes of Medical Sciences, Banaras Hindu University, Varanasi, India
    N Engl J Med 347:1739-46. 2002
  5. ncbi Perifosine: update on a novel Akt inhibitor
    Joell J Gills
    Medical Oncology Branch, National Cancer Institute, NNMC Building 8, Room 5101, 8901 Wisconsin Avenue, Bethesda, MD 20889, USA
    Curr Oncol Rep 11:102-10. 2009
  6. ncbi Synergistic induction of apoptosis in human leukemia T cells by the Akt inhibitor perifosine and etoposide through activation of intrinsic and Fas-mediated extrinsic cell death pathways
    Maria Nyakern
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Mol Cancer Ther 5:1559-70. 2006
  7. doi Low levels of IgM antibodies to phosphorylcholine predict cardiovascular disease in 60-year old men: effects on uptake of oxidized LDL in macrophages as a potential mechanism
    Ulf de Faire
    Division of Cardiovascular Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Autoimmun 34:73-9. 2010
  8. doi Miltefosine as an effective choice in the treatment of post-kala-azar dermal leishmaniasis
    V Ramesh
    Department of Dermatology, Safdarjung Hospital Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Br J Dermatol 165:411-4. 2011
  9. pmc Miltefosine affects lipid metabolism in Leishmania donovani promastigotes
    M Rakotomanga
    Chimiothérapie Antiparasitaire, UMR 8076 CNRS, Faculte de Pharmacie, Universite Paris Sud XI, F 92290 Chatenay Malabry, France
    Antimicrob Agents Chemother 51:1425-30. 2007
  10. doi Comparison of short-course multidrug treatment with standard therapy for visceral leishmaniasis in India: an open-label, non-inferiority, randomised controlled trial
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Lancet 377:477-86. 2011

Detail Information

Publications375 found, 100 shown here

  1. ncbi Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda
    Philippe J Guerin
    Norwegian Institute of Public Health, Norway
    Lancet Infect Dis 2:494-501. 2002
    ..This article reviews the current situation and perspectives for diagnosis, treatment, and control of visceral leishmaniasis, and lists some priorities for research and development...
  2. pmc Miltefosine induces apoptosis-like death in Leishmania donovani promastigotes
    Caroline Paris
    UMR 8076 CNRS, Chimiothérapie Antiparasitaire, France
    Antimicrob Agents Chemother 48:852-9. 2004
    ..Identification of the death-signaling pathways activated in HePC-sensitive parasites appears to be essential for a better understanding of the molecular mechanisms of action and resistance in these parasites...
  3. pmc Phosphorylcholine decreases early inflammation and promotes the establishment of stable biofilm communities of nontypeable Haemophilus influenzae strain 86-028NP in a chinchilla model of otitis media
    Wenzhou Hong
    Department of Microbiology and Immunology, Wake Forest University Health Sciences, Winston Salem, NC 27157, USA
    Infect Immun 75:958-65. 2007
    ..b>Phosphorylcholine (PCho) is added to some LOS forms in a phase-variable manner, and these PCho(+) variants predominate in vivo...
  4. ncbi Oral miltefosine for Indian visceral leishmaniasis
    Shyam Sundar
    Institutes of Medical Sciences, Banaras Hindu University, Varanasi, India
    N Engl J Med 347:1739-46. 2002
    ..We performed a clinical trial in India comparing miltefosine with the most effective standard treatment, amphotericin B...
  5. ncbi Perifosine: update on a novel Akt inhibitor
    Joell J Gills
    Medical Oncology Branch, National Cancer Institute, NNMC Building 8, Room 5101, 8901 Wisconsin Avenue, Bethesda, MD 20889, USA
    Curr Oncol Rep 11:102-10. 2009
    ..In this article, we review the clinical history of perifosine and discuss its many biologic activities...
  6. ncbi Synergistic induction of apoptosis in human leukemia T cells by the Akt inhibitor perifosine and etoposide through activation of intrinsic and Fas-mediated extrinsic cell death pathways
    Maria Nyakern
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Mol Cancer Ther 5:1559-70. 2006
    ..These findings might be useful when designing clinical trials where chemotherapy is combined with perifosine for a potential broad use against hematologic malignancies in which the Akt survival pathway is frequently activated...
  7. doi Low levels of IgM antibodies to phosphorylcholine predict cardiovascular disease in 60-year old men: effects on uptake of oxidized LDL in macrophages as a potential mechanism
    Ulf de Faire
    Division of Cardiovascular Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Autoimmun 34:73-9. 2010
    We here determine the role of IgM antibodies against phosphorylcholine (anti-PC) in prediction of cardiovascular disease (CVD) and on macrophage uptake of Oxidized LDL (OxLDL).
  8. doi Miltefosine as an effective choice in the treatment of post-kala-azar dermal leishmaniasis
    V Ramesh
    Department of Dermatology, Safdarjung Hospital Institute of Pathology ICMR, Safdarjung Hospital Campus, New Delhi 110029, India
    Br J Dermatol 165:411-4. 2011
    ..Unacceptable treatment regimens and increasing drug resistance blight control programmes. The success of oral miltefosine in VL prompted a clinical, histopathological and parasitological study of this drug in PKDL...
  9. pmc Miltefosine affects lipid metabolism in Leishmania donovani promastigotes
    M Rakotomanga
    Chimiothérapie Antiparasitaire, UMR 8076 CNRS, Faculte de Pharmacie, Universite Paris Sud XI, F 92290 Chatenay Malabry, France
    Antimicrob Agents Chemother 51:1425-30. 2007
    ....
  10. doi Comparison of short-course multidrug treatment with standard therapy for visceral leishmaniasis in India: an open-label, non-inferiority, randomised controlled trial
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
    Lancet 377:477-86. 2011
    ..Improved treatment approaches are needed for visceral leishmaniasis. We assessed the efficacy and safety of three potential short-course combination treatments compared with the standard monotherapy in India...
  11. pmc Perifosine inhibits mammalian target of rapamycin signaling through facilitating degradation of major components in the mTOR axis and induces autophagy
    Lei Fu
    Department of Hematology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 69:8967-76. 2009
    ....
  12. ncbi Passive immunization with monoclonal IgM antibodies against phosphorylcholine reduces accelerated vein graft atherosclerosis in apolipoprotein E-null mice
    Jose R Faria-Neto
    Atherosclerosis Research Center, Division of Cardiology, Department of Medicine and Burns and Allen Research Institute, Cedars Sinai Medical Center and David Geffen School of Medicine at UCLA, Los Angeles, CA 90048, USA
    Atherosclerosis 189:83-90. 2006
    b>Phosphorylcholine (PC) headgroup is one of the neoantigens exposed by LDL oxidation that can elicit an immune response...
  13. pmc In vitro susceptibility of field isolates of Leishmania donovani to Miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity
    Dhiraj Kumar
    Institute of Pathology, Safdarjung Hospital Campus, New Delhi, India
    Antimicrob Agents Chemother 53:835-8. 2009
    ..The results of gene expression analysis of LdMT and LdRos3 were suggestive of alternate mechanisms of Miltefosine susceptibility in the isolates...
  14. ncbi Characterisation of Leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine)
    Karin Seifert
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Antimicrob Agents 22:380-7. 2003
    ..Resistance was stable up to 12 weeks in drug-free culture medium. No amplification of specific genes, including the multidrug resistance P-glycoprotein gene, could be detected in the resistant parasites...
  15. pmc Synergistic proapoptotic activity of recombinant TRAIL plus the Akt inhibitor Perifosine in acute myelogenous leukemia cells
    Pier Luigi Tazzari
    Servizio di Immunoematologia e Trasfusionale, Policlinico S Orsola Malpighi, Universita di Bologna, Italy
    Cancer Res 68:9394-403. 2008
    ..In contrast, CD34(+) cells from healthy donors were resistant to perifosine and TRAIL treatment. Our findings suggest that the combination of perifosine and TRAIL might offer a novel therapeutic strategy for AML...
  16. doi Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells
    Irene Canton
    The Krebs Institute, University of Sheffield, Sheffield, UK
    FASEB J 27:98-108. 2013
    ..This delivery system is based on poly[2-(methacryloyloxy)ethyl phosphorylcholine]-block-[2-(diisopropylamino)ethyl methacrylate] (PMPC-PDPA), a pH-sensitive diblock copolymer that self-..
  17. ncbi Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation
    Jose M Mancheno
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Structure 11:1319-28. 2003
    ..These results provide the structural basis for the membrane recognition step of actinoporins and unexpected insights into the oligomerization step...
  18. doi A Rho kinase/myocardin-related transcription factor-A-dependent mechanism underlies the sphingosylphosphorylcholine-induced differentiation of mesenchymal stem cells into contractile smooth muscle cells
    Eun Su Jeon
    Department of Physiology, Pusan National University College of Medicine, Busan 602 739, Republic of Korea
    Circ Res 103:635-42. 2008
    ..These results suggest that SPC induced differentiation of hADSCs into contractile SMCs through a mechanism involving RhoA/Rho kinase-dependent nuclear translocation of MRTF-A...
  19. ncbi Phosphocholine of pneumococcal teichoic acids: role in bacterial physiology and pneumococcal infection
    W Fischer
    Institut fur Biochemie, Med Fak, Universitat Erlangen Nurnberg, Erlangen, Germany
    Res Microbiol 151:421-7. 2000
    ..The genes encoding the enzymes for PCho incorporation are described. Two strains that acquired the ability to grow in the absence of choline are discussed...
  20. doi Akt down-modulation induces apoptosis of human prostate cancer cells and synergizes with EGFR tyrosine kinase inhibitors
    Claudio Festuccia
    Departments of Experimental Medicine, University of L Aquila, L Aquila, Italy
    Prostate 68:965-74. 2008
    ..PTEN is a well-characterized tumor suppressor that negatively regulates cell growth and survival through the modulation of PI3K/Akt pathway...
  21. ncbi Intranasal immunization with phosphorylcholine induces antigen specific mucosal and systemic immune responses in mice
    Norimitsu Tanaka
    Department of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
    Vaccine 25:2680-7. 2007
    b>Phosphorylcholine (PC) is a structural component of a wide variety of pathogens including Streptococcus pneumoniae and Haemophilus influenzae, and anti-PC immune responses are known to protect mice against invasive bacterial diseases...
  22. ncbi Oral miltefosine treatment in children with mild to moderate Indian visceral leishmaniasis
    Shyam Sundar
    Institutes of Medical Sciences, Banaras Hindu University, 6 SK Gupta Nagar, Varanasi 221005, India
    Pediatr Infect Dis J 22:434-8. 2003
    ..Because approximately one-half of the visceral leishmaniasis patients worldwide are children, we performed a Phase I/II dose ranging study in the pediatric population in India...
  23. ncbi B-1 cells: the lineage question revisited
    L A Herzenberg
    Department of Genetics, Stanford University School of Medicine, CA 94305, USA
    Immunol Rev 175:9-22. 2000
    ....
  24. ncbi Development of miltefosine as an oral treatment for leishmaniasis
    H Sindermann
    Zentaris GmbH, Weismuellerstrasse 50, 60314 Frankfurt, Germany
    Trans R Soc Trop Med Hyg 100:S17-20. 2006
    ..It is therefore contraindicated for use during pregnancy, and contraception is required beyond the end of treatment in women of child-bearing age...
  25. pmc Pharmacokinetics of miltefosine in Old World cutaneous leishmaniasis patients
    Thomas P C Dorlo
    Department of Pharmacy and Pharmacology, Slotervaart Hospital The Netherlands Cancer Institute, Louwesweg 6, 1066 EC, Amsterdam, The Netherlands
    Antimicrob Agents Chemother 52:2855-60. 2008
    ....
  26. ncbi Sensitivities of Leishmania species to hexadecylphosphocholine (miltefosine), ET-18-OCH(3) (edelfosine) and amphotericin B
    Patricia Escobar
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Acta Trop 81:151-7. 2002
    ..5-37.1 microM against amastigotes. Amphotericin B deoxycholate was used as the standard drug and gave submicromolar ED(50) values in all assays; L. mexicana was the least sensitive species to this drug...
  27. ncbi Edelfosine and perifosine induce selective apoptosis in multiple myeloma by recruitment of death receptors and downstream signaling molecules into lipid rafts
    Consuelo Gajate
    Unidad de Investigacion, Hospital Universitario de Salamanca, Spain
    Blood 109:711-9. 2007
    ..These data uncover a novel lipid raft-mediated therapy in MM involving concentration of death receptors in membrane rafts, with Fas/CD95 playing a major role in ALP-mediated apoptosis...
  28. ncbi Efficacy of miltefosine for Bolivian cutaneous leishmaniasis
    Jaime Soto
    Fundación FADER, Bogota, Colombia
    Am J Trop Med Hyg 78:210-1. 2008
    ..braziliensis in this region than for L. braziliensis in Guatemala. Chemotherapy needs to be evaluated in each endemic region, even if the "same" species of Leishmania causes disease in these locales...
  29. pmc In vitro and in vivo inhibition of neuroblastoma tumor cell growth by AKT inhibitor perifosine
    Zhijie Li
    Cell and Molecular Biology Section, Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health, 10 Center Drive MSC 1928, Bldg 10 CRC 1 3940, Bethesda, MD 20892, USA
    J Natl Cancer Inst 102:758-70. 2010
    ..The aim of this study was to investigate the effect of perifosine, a nontoxic AKT inhibitor, as a single agent on NB cell growth in vitro and in vivo...
  30. pmc Phosphorylcholine expression by nontypeable Haemophilus influenzae correlates with maturation of biofilm communities in vitro and in vivo
    Wenzhou Hong
    Department of Microbiology and Immunology, Wake Forest University Health Sciences, 5101A Gray Building, Medical Center Boulevard, Winston Salem, NC 27157, USA
    J Bacteriol 189:8300-7. 2007
    ..NTHI variants within biofilms have on their surfaces lipooligosaccharides containing sialic acid (NeuAc) and phosphorylcholine (PCho)...
  31. ncbi Detection of increased choline compounds with proton nuclear magnetic resonance spectroscopy subsequent to malignant transformation of human prostatic epithelial cells
    E Ackerstaff
    Magnetic Resonance Oncology Section, Division of Magnetic Resonance Research, Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cancer Res 61:3599-603. 2001
    ..These data strongly support the use of proton magnetic resonance spectroscopic imaging to detect the presence of prostate cancer for diagnosis, to detect response subsequent to androgen ablation therapy, and to detect recurrence...
  32. doi Visceral leishmaniasis: elimination with existing interventions
    Greg Matlashewski
    Special Programme for Research and Training in Tropical Diseases, WHO, Geneva, Switzerland
    Lancet Infect Dis 11:322-5. 2011
    ....
  33. ncbi Inactivation of the miltefosine transporter, LdMT, causes miltefosine resistance that is conferred to the amastigote stage of Leishmania donovani and persists in vivo
    Karin Seifert
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Antimicrob Agents 30:229-35. 2007
    ..90% inhibition in wild-type infections). No cross-resistance to other antileishmanial drugs was observed in M-mutR amastigotes...
  34. pmc Lipooligosaccharides containing phosphorylcholine delay pulmonary clearance of nontypeable Haemophilus influenzae
    Bing Pang
    Department of Microbiology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Infect Immun 76:2037-43. 2008
    ..Persistent NTHi populations express a discrete subset of LOS glycoforms, including those containing phosphorylcholine (PCho)...
  35. pmc Alteration of fatty acid and sterol metabolism in miltefosine-resistant Leishmania donovani promastigotes and consequences for drug-membrane interactions
    M Rakotomanga
    Chimiothérapie Antiparasitaire, UMR 8076 CNRS, Faculte de Pharmacie, Universite Paris XI, F 92290, Chatenay Malabry, France
    Antimicrob Agents Chemother 49:2677-86. 2005
    ..In conclusion, HePC resistance affects three lipid biochemical pathways: fatty acid elongation, the desaturase system responsible for fatty acid alkyl chain unsaturation, and the C-24-alkylation of sterols...
  36. ncbi Phase II study of perifosine in previously untreated patients with metastatic melanoma
    D Scott Ernst
    London Regional Cancer Centre, 790 Commissioners Rd East, London, ON N6A 4L6, UK
    Invest New Drugs 23:569-75. 2005
    ..To assess the response rate and toxicity of the alkylphosphocholine analogue, perifosine, in patients with metastatic or recurrent malignant melanoma...
  37. pmc Possible mechanism of miltefosine-mediated death of Leishmania donovani
    Navin K Verma
    Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Punjab, India
    Antimicrob Agents Chemother 48:3010-5. 2004
    ..Understanding of miltefosine-mediated death will facilitate the design of new therapeutic strategies against Leishmania parasites...
  38. ncbi Phase 4 trial of miltefosine for the treatment of Indian visceral leishmaniasis
    Sujit Kumar Bhattacharya
    Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, India
    J Infect Dis 196:591-8. 2007
    ..Hospital-based studies have shown that oral miltefosine is a highly effective treatment for VL both in adults and in children...
  39. ncbi Mechanisms of experimental resistance of Leishmania to miltefosine: Implications for clinical use
    F Javier Pérez-Victoria
    Instituto de Parasitologia y Biomedicina Lopez Neyra, Spanish Research Council C S I C, Technological Park of Health Sciences, Avda del Conocimiento s n, 18100 Armilla, Granada, Spain
    Drug Resist Updat 9:26-39. 2006
    ....
  40. pmc Randomized controlled clinical trial to access efficacy and safety of miltefosine in the treatment of cutaneous leishmaniasis Caused by Leishmania (Viannia) guyanensis in Manaus, Brazil
    Anette Chrusciak-Talhari
    Fundação de Medicina Tropical do Amazonas and Universidade Estadual do Amazonas, Manaus, AM, Brasil
    Am J Trop Med Hyg 84:255-60. 2011
    ..05) for miltefosine and antimonial, respectively. There were no differences in cure rates between age groups within the same treatment arms. Miltefosine was safe and relatively well tolerated and cure rate was higher than antimony...
  41. pmc Phosphorylcholine on the lipopolysaccharide of Haemophilus influenzae contributes to persistence in the respiratory tract and sensitivity to serum killing mediated by C-reactive protein
    J N Weiser
    Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Exp Med 187:631-40. 1998
    Haemophilus influenzae undergoes phase variation in expression of the phosphorylcholine (ChoP) epitope, a structure present on several invasive pathogens residing in the human respiratory tract...
  42. doi Low level natural antibodies against phosphorylcholine: a novel risk marker and potential mechanism in atherosclerosis and cardiovascular disease
    Johan Frostegard
    Department of Medicine, Karolinska University Hospital, Huddinge, Karolinska Institutet, Stockholm, Sweden
    Clin Immunol 134:47-54. 2010
    ..A common denominator is phosphorylcholine (PC), a hapten-like epitope which is exposed on OxLDL and some microorganisms...
  43. ncbi Magnetic resonance spectroscopy monitoring of mitogen-activated protein kinase signaling inhibition
    Mounia Beloueche-Babari
    Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey SM2 5PT, UK
    Cancer Res 65:3356-63. 2005
    ..Thus, phosphocholine has potential as a noninvasive pharmacodynamic marker for monitoring MAPK signaling blockade...
  44. pmc Rheumatoid cachexia is associated with dyslipidemia and low levels of atheroprotective natural antibodies against phosphorylcholine but not with dietary fat in patients with rheumatoid arthritis: a cross-sectional study
    Ann Charlotte Elkan
    Department of Rheumatology, Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Arthritis Res Ther 11:R37. 2009
    ..The aim of this study was to analyze the associations between diet, body composition, lipids and atheroprotective natural antibodies against phosphorylcholine (anti-PC) in patients with RA.
  45. ncbi Perifosine inhibits multiple signaling pathways in glial progenitors and cooperates with temozolomide to arrest cell proliferation in gliomas in vivo
    Hiroyuki Momota
    Department of Cancer Biology and Genetics and Surgery Neurosurgery and Neurology, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cancer Res 65:7429-35. 2005
    ..01). These results indicate that perifosine is an effective drug in gliomas in which Akt and Ras-Erk 1/2 pathways are frequently activated, and may be a new candidate for glioma treatment in the clinic...
  46. pmc Decoration of lipopolysaccharide with phosphorylcholine: a phase-variable characteristic of Haemophilus influenzae
    J N Weiser
    Department of Pediatrics, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, 19104 4318, USA
    Infect Immun 65:943-50. 1997
    ..Incorporated choline is in the form of phosphorylcholine (ChoP) based on the reactivity with the monoclonal antibody with specificity for this structure, TEPC-15...
  47. ncbi Phosphorylcholine-based pH-responsive diblock copolymer micelles as drug delivery vehicles: light scattering, electron microscopy, and fluorescence experiments
    Cristiano Giacomelli
    Laboratoire de Chimie des Polymeres Organiques, LCPO ENSCPB, Universite Bordeaux 1, 16 Av Pey Berland, 33607 Pessac Cedex, France
    Biomacromolecules 7:817-28. 2006
    ..of a diblock copolymer comprising a highly hydrophilic and biocompatible poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) corona-forming block and a pH-sensitive poly(2-(diisopropylamino)ethyl methacrylate) (PDPA) core-..
  48. ncbi Streptococcus pneumoniae anchor to activated human cells by the receptor for platelet-activating factor
    D R Cundell
    Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, New York 10021 6399, USA
    Nature 377:435-8. 1995
    ..Only virulent pneumococci engage the PAF receptor. Attachment of the bacterial phosphorylcholine to the PAF receptor enhanced adherence, which was coupled to invasion of endothelial, epithelial and PAF-..
  49. pmc Presence of phosphorylcholine on a filarial nematode protein influences immunoglobulin G subclass response to the molecule by an interleukin-10-dependent mechanism
    K M Houston
    Department of Immunology, University of Strathclyde, Glasgow G4 0NR, United Kingdom
    Infect Immun 68:5466-8. 2000
    The filarial nematode product ES-62 contains phosphorylcholine (PC) covalently attached to N-linked glycans...
  50. pmc A novel small molecule antagonist of choline kinase-α that simultaneously suppresses MAPK and PI3K/AKT signaling
    B F Clem
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    Oncogene 30:3370-80. 2011
    ....
  51. doi Natural antibodies against phosphorylcholine as potential protective factors in SLE
    J Su
    Rheumatology Unit, Karolinska University Hospital, Huddinge, Karolinska Institutet corrected Stockholm, Sweden
    Rheumatology (Oxford) 47:1144-50. 2008
    We have recently reported that natural antibodies against phosphorylcholine (anti-PC) have atheroprotective properties. Here we compare anti-PC with other autoantibodies in SLE patients with and without cardiovascular disease (CVD).
  52. ncbi Involvement of Src family protein tyrosine kinases in Ca(2+) sensitization of coronary artery contraction mediated by a sphingosylphosphorylcholine-Rho-kinase pathway
    Fumiaki Nakao
    Department of Cardiovascular Medicine, Yamaguchi University School of Medicine, Yamaguchi, Japan
    Circ Res 91:953-60. 2002
    ..Translocation and activation of SrcPTKs, including Fyn, play an important role in Ca(2+) sensitization of VSM contractions mediated by a SPC-Rho-kinase pathway...
  53. doi Balance of human choline kinase isoforms is critical for cell cycle regulation: implications for the development of choline kinase-targeted cancer therapy
    Jens Gruber
    Enzyme Biochemistry Research Group, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany
    FEBS J 279:1915-28. 2012
    The enzyme choline kinase (CK), which catalyzes the phosphorylation of choline to phosphorylcholine in the presence of ATP, has an essential role in the biosynthesis of phosphatidylcholine, the major constituent of all mammalian cell ..
  54. ncbi A phosphorylcholine-containing filarial nematode-secreted product disrupts B lymphocyte activation by targeting key proliferative signaling pathways
    M R Deehan
    Department of Immunology, University of Strathclyde, Glasgow, United Kingdom
    J Immunol 160:2692-9. 1998
    ..In this study, we demonstrate that ES-62 (2 microg/ml), a phosphorylcholine (PC)-containing glycoprotein released by the rodent filarial parasite Acanthocheilonema viteae, is able to ..
  55. ncbi Intracellular mediators of erucylphosphocholine-induced apoptosis
    Verena Jendrossek
    Department of Radiation Oncology, University of Tubingen, Hoppe Seyler Str 3, D 72076 Tubingen, Germany
    Oncogene 22:2621-31. 2003
    ..Thus, ErPC triggers apoptosis via a Bcl-2-dependent mitochondrial but death receptor-independent pathway...
  56. doi Co-nonsolvency effects for surface-initiated poly(2-(methacryloyloxy)ethyl phosphorylcholine) brushes in alcohol/water mixtures
    Steve Edmondson
    Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, South Yorkshire S3 7HF, United Kingdom
    Langmuir 26:7216-26. 2010
    ..transfer radical polymerization (SI-ATRP) has been used to grow brushes of poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC) from silicon wafers using a polyelectrolytic macroinitiator on planar silicon wafers...
  57. doi Novel phosphorylcholine-containing protein of Pseudomonas aeruginosa chronic infection isolates interacts with airway epithelial cells
    Mariette Barbier
    Institut Universitari d Investigacions en Ciències de la Salut, Palma de Mallorca, Spain
    J Infect Dis 197:465-73. 2008
    Pseudomonas aeruginosa undergoes phase variation in the expression of the phosphorylcholine (ChoP) epitope, a structure crucial for the virulence of several respiratory pathogens...
  58. pmc Histone deacetylase inhibition increases levels of choline kinase α and phosphocholine facilitating noninvasive imaging in human cancers
    Mounia Beloueche-Babari
    Cancer Research UK and EPSRC Cancer Imaging Centre, Division of Radiotherapy and Imaging, The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, Sutton, Surrey, United Kingdom
    Cancer Res 72:990-1000. 2012
    ..Together, our findings provide mechanistic insights into the impact of HDAC inhibition on cancer cell metabolism and highlight PC as a candidate noninvasive imaging biomarker for monitoring the action of HDAC inhibitors...
  59. pmc IgM antibodies to apoptosis-associated determinants recruit C1q and enhance dendritic cell phagocytosis of apoptotic cells
    Yifang Chen
    Department of Medicine, Laboratory of B Cell Immunobiology, University of California at San Diego, La Jolla, CA 92093, USA
    J Immunol 182:6031-43. 2009
    ..to neoantigenic determinants on apoptotic thymocytes is dominated by Abs to oxidation-associated Ags, phosphorylcholine (PC), a head group that becomes exposed during programmed cell death, and malondialdehyde (MDA), a reactive ..
  60. pmc Platelet-activating factor receptor initiates contact of Acinetobacter baumannii expressing phosphorylcholine with host cells
    Younes Smani
    Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville IBIS, University Hospital Virgen del Rocío CSIC University of Seville, 41013 Seville, Spain
    J Biol Chem 287:26901-10. 2012
    ..baumannii and the host cells remains unclear. Here, we demonstrated that the phosphorylcholine (ChoP)-containing outer membrane protein of A...
  61. doi Substrate recognition of a structure motif for phosphorylcholine post-translational modification in Neisseria meningitidis
    Freda E C Jen
    Institute for Glycomics, Griffith University, Gold Coast Campus, Qld 4222, Australia
    Biochem Biophys Res Commun 431:808-14. 2013
    ..meningitidis. In this study, we investigate the substrate recognition of phosphorylcholine transferase...
  62. doi IgM-phosphorylcholine autoantibodies and outcome in acute coronary syndromes
    Kenneth Caidahl
    Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden
    Int J Cardiol 167:464-9. 2013
    Antibodies against proinflammatory phosphorylcholine (anti-PC) seem to be protective and reduce morbidity...
  63. ncbi Metabolic markers of breast cancer: enhanced choline metabolism and reduced choline-ether-phospholipid synthesis
    Rachel Katz-Brull
    Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel
    Cancer Res 62:1966-70. 2002
    ..Uniquely, reduced levels and synthesis of a choline-ether-phospholipid may also serve as a metabolic marker of breast cancer...
  64. pmc Highly reduced protection against Streptococcus pneumoniae after deletion of a single heavy chain gene in mouse
    Q S Mi
    Laboratory of Immunology, B Cell Development Section, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Proc Natl Acad Sci U S A 97:6031-6. 2000
    ....
  65. doi Molecular simulation studies of protein interactions with zwitterionic phosphorylcholine self-assembled monolayers in the presence of water
    Yi He
    Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
    Langmuir 24:10358-64. 2008
    Molecular simulations were performed to study the interactions between a protein (lysozyme, LYZ) and phosphorylcholine-terminated self-assembled monolayers (PC-SAMs) in the presence of explicit water molecules and ions...
  66. doi Autoantibodies against apolipoprotein A-1 and phosphorylcholine for diagnosis of non-ST-segment elevation myocardial infarction
    P F Keller
    Division of Cardiology, Department of Internal Medicine, Geneva University Hospitals, Geneva, Switzerland
    J Intern Med 271:451-62. 2012
    To explore the diagnostic accuracies of anti-apolipoproteinA-1 (anti-ApoA-1) IgG and anti-phosphorylcholine (anti-PC) IgM alone, expressed as a ratio (anti-ApoA-1 IgG/anti-PC IgM), and combined with the Thrombolysis In Myocardial ..
  67. pmc Induction of thyroid gene expression and radioiodine uptake in thyroid cancer cells by targeting major signaling pathways
    Peng Hou
    Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, 1830 East Monument Street, Suite 333, Baltimore, Maryland 21287, USA
    J Clin Endocrinol Metab 95:820-8. 2010
    ..Radioiodine ablation is commonly used to treat thyroid cancer, but a major challenge is often the loss of radioiodine avidity of the cancer caused by aberrant silencing of iodide-handling genes...
  68. pmc Perifosine induces protective autophagy and upregulation of ATG5 in human chronic myelogenous leukemia cells in vitro
    Yin Tong
    Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
    Acta Pharmacol Sin 33:542-50. 2012
    ..In this study, the cytotoxic effects of perifosine on CML and acute myeloid leukemia (AML) cell lines were compared to elucidate the mechanisms underlying the differences...
  69. ncbi Relapse of visceral leishmaniasis after miltefosine treatment in a Nepalese patient
    Basu Dev Pandey
    Sukraraj Tropical and Infectious Diseases Hospital, Kathmandu, Nepal
    Am J Trop Med Hyg 80:580-2. 2009
    ..The patient was subsequently treated with 1 mg/kg of amphotericin B for a total of 14 days and recovered completely...
  70. ncbi Physical properties and pharmacological activity in vitro and in vivo of optimised liposomes prepared from a new cancerostatic alkylphospholipid
    R Zeisig
    Phospholipids Department, Max Delbruck Centre for Molecular Medicine, R Rössle Str 10, 13122 Berlin Buch, Germany
    Biochim Biophys Acta 1414:238-48. 1998
    ..The beneficial therapeutic effect of these liposomes was accompanied by better tolerance and a significant inhibition of haemolysis compared to micellar OPP...
  71. ncbi Effect of biological therapy on levels of atheroprotective antibodies against phosphorylcholine and apolipoproteins in rheumatoid arthritis - a one year study
    Sofia Ajeganova
    Karolinska University Hospital Huddinge, Stockholm, Sweden
    Clin Exp Rheumatol 29:942-50. 2011
    ..B-cell targeting rituximab influences novel markers of atherosclerosis and inflammation, such as atheroprotective natural IgM antibodies against phosphorylcholine (anti-PC), oxidised low-density lipoprotein (oxLDL) and apolipoproteins.
  72. ncbi Characterisation of the phosphorylcholine-containing N-linked oligosaccharides in the excretory-secretory 62 kDa glycoprotein of Acanthocheilonema viteae
    S M Haslam
    Department of Biochemistry, Imperial College, London, UK
    Mol Biochem Parasitol 85:53-66. 1997
    ..product of the rodent filarial nematode Acanthocheilonema viteae is a 62 kDa glycoprotein (ES-62), which has phosphorylcholine, attached to the N-linked carbohydrates...
  73. ncbi Alkylphospholipids inhibit capillary-like endothelial tube formation in vitro: antiangiogenic properties of a new class of antitumor agents
    Shuraila F Zerp
    Division of Cellular Biochemistry, Department of Pathology, The Netherlands Cancer Institute Antoni van Leeuwenhoek Hospital, Plesmanlaan, CX Amsterdam, The Netherlands
    Anticancer Drugs 19:65-75. 2008
    ..Interference with the angiogenic process represents a novel mode of action of APLs and might significantly contribute to the antitumor effect of these compounds...
  74. pmc Perifosine as a potential novel anti-cancer agent inhibits EGFR/MET-AKT axis in malignant pleural mesothelioma
    Giulia Pinton
    Department of Pharmaceutical Sciences and Drug and Food Biotechnology Center, University of Piemonte Orientale A Avogadro, Novara, Italy
    PLoS ONE 7:e36856. 2012
    ....
  75. ncbi Cure of antimony-unresponsive Indian post-kala-azar dermal leishmaniasis with oral miltefosine
    Shyam Sundar
    Kala azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India
    Trans R Soc Trop Med Hyg 100:698-700. 2006
    ..Oral miltefosine appears to be an important alternative for the treatment of PKDL in India and confirmatory studies in controlled clinical trials are needed...
  76. ncbi Piezoelectric immunosensor for bisphenol A based on signal enhancing step with 2-methacrolyloxyethyl phosphorylcholine polymeric nanoparticle
    Jong Won Park
    Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology AIST, 1 1Higashi, Tsukuba 305 8565, Japan
    Analyst 131:155-62. 2006
    ..Field emission-scanning electron microscopy (FE-SEM) showed that polymeric 2-methacrolyloxyethyl phosphorylcholine (MPC) nanoparticles coupled to antibodies remained monodisperse on the surface of the immunosensor and ..
  77. ncbi Phase II study of daily oral perifosine in patients with advanced soft tissue sarcoma
    Howard H Bailey
    University of Wisconsin Comprehensive Cancer Center, Madison, Wisconsin 53792, USA
    Cancer 107:2462-7. 2006
    ....
  78. pmc Microbial modulation of host immunity with the small molecule phosphorylcholine
    Sarah E Clark
    Department of Microbiology, University of Pennsylvania, Philadelphia, PA, USA
    Infect Immun 81:392-401. 2013
    ..The small molecule phosphorylcholine, or choline phosphate (ChoP), is used for both of these purposes by a wide array of bacterial and parasitic ..
  79. ncbi Phosphocholine and sphingosine-1-phosphate synergistically stimulate DNA synthesis by a MAP kinase-dependent mechanism
    Z Kiss
    The Hormel Institute, University of Minnesota, Austin 55912, USA
    FEBS Lett 412:197-200. 1997
    ....
  80. ncbi Coadministration of histone deacetylase inhibitors and perifosine synergistically induces apoptosis in human leukemia cells through Akt and ERK1/2 inactivation and the generation of ceramide and reactive oxygen species
    Mohamed Rahmani
    Department of Medicine, Virginia Commonwealth University, School of Medicine, Richmond, VA 23298, USA
    Cancer Res 65:2422-32. 2005
    ..They also raise the possibility that combining these agents may represent a novel antileukemic strategy...
  81. doi Miltefosine, a promising novel agent for schistosomiasis mansoni
    Maha M Eissa
    Department of Medical Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt
    Int J Parasitol 41:235-42. 2011
    ..In conclusion, we believe this is the first study highlighting miltefosine as a promising novel agent for schistosomiasis mansoni...
  82. doi Perifosine synergistically enhances TRAIL-induced myeloma cell apoptosis via up-regulation of death receptors
    Ebenezer David
    Department of Hematology and Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia, USA
    Clin Cancer Res 14:5090-8. 2008
    ..The aim of this study is to investigate the efficacy of a novel Akt inhibitor, perifosine, in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in human myeloma cells and primary patient samples...
  83. ncbi Proapoptotic activity and chemosensitizing effect of the novel Akt inhibitor perifosine in acute myelogenous leukemia cells
    V Papa
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Universita di Bologna, Bologna, Italy
    Leukemia 22:147-60. 2008
    ..Our findings indicate that perifosine, either alone or in combination with existing drugs, might be a promising therapeutic agent for the treatment of those AML cases characterized by upregulation of the PI3K-Akt survival pathway...
  84. ncbi Increased junctional diversity in fetal B cells results in a loss of protective anti-phosphorylcholine antibodies in adult mice
    C L Benedict
    Department of Microbiology, University of Alabama at Birmingham, 35294 3300, USA
    Immunity 10:607-17. 1999
    ..These results show that maintenance of lower Ig diversity in early life is essential for the acquisition of a complete functional adult repertoire...
  85. doi Changes in choline metabolism as potential biomarkers of phospholipase C{gamma}1 inhibition in human prostate cancer cells
    Mounia Beloueche-Babari
    Cancer Research UK Clinical Magnetic Resonance Research Group, The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, Sutton, Surrey, United Kingdom
    Mol Cancer Ther 8:1305-11. 2009
    ....
  86. ncbi First case of typical Old World cutaneous leishmaniasis treated with miltefosine
    Marija Stojkovic
    Section of Clinical Tropical Medicine, Heidelberg University Hospital, Heidelberg, and General Practice, Dreisamstr 11, Freiburg, Germany
    Int J Dermatol 46:385-7. 2007
    ..Ten days after completion of treatment, the rolled edge had disappeared and the central ulcer had almost healed (Fig. 3). Figure 4 shows the lesion 5 months after completion of treatment...
  87. ncbi Anticancer alkylphospholipids: mechanisms of action, cellular sensitivity and resistance, and clinical prospects
    Wim J van Blitterswijk
    Division of Cellular Biochemistry, Department of Radiotherapy, Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands
    Curr Pharm Des 14:2061-74. 2008
    ..For example, ALPs increase the cancer cell sensitivity to radiotherapy in vitro and in vivo. We highlight the clinical potential of perifosine, an orally available APL...
  88. ncbi Treatment of New World cutaneous leishmaniasis with miltefosine
    J Soto
    Consorcio de Investigaciones Bioclinicas, Calle 60 A 5 54 Suite 201, Bogota, Colombia
    Trans R Soc Trop Med Hyg 100:S34-40. 2006
    ..Miltefosine was well tolerated, and as effective as historic values of antimony for treatment of L. panamensis disease...
  89. pmc Increased prevalence of vulnerable atherosclerotic plaques and low levels of natural IgM antibodies against phosphorylcholine in patients with systemic lupus erythematosus
    Cristina Anania
    Department of Medicine, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden
    Arthritis Res Ther 12:R214. 2010
    ..We recently reported a negative association between natural IgM-antibodies against phosphorylcholine (anti-PC) in the general population, high anti-PC levels leading to decreased atherosclerosis development ..
  90. pmc Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment
    David Gallego-Ortega
    Translational Oncology Unit, CSIC UAM La Paz, Instituto de Investigaciones Biomedicas, Madrid, Spain
    PLoS ONE 4:e7819. 2009
    ..Recently ChoKalpha has been implicated in the carcinogenic process, since it is over-expressed in a variety of human cancers. However, no evidence for a role of ChoKbeta in carcinogenesis has been reported...
  91. doi Anti-acanthamoeba efficacy and toxicity of miltefosine in an organotypic skin equivalent
    Julia Walochnik
    Department of Medical Parasitology, Institute of Hygiene, Medical University of Vienna, 1095 Vienna, Austria
    J Antimicrob Chemother 64:539-45. 2009
    ..in vitro. The aim of the current study was to evaluate the suitability of miltefosine for the topical treatment of Acanthamoeba infections...
  92. doi Sphingosylphosphorylcholine inhibits melanin synthesis via pertussis toxin-sensitive MITF degradation
    Dong Seok Kim
    Department of Biochemistry, Chung Ang University College of Medicine, Seoul, Republic of Korea
    J Pharm Pharmacol 62:181-7. 2010
    ..In this study we aimed to examine sphingosylphosphorylcholine-mediated signalling pathways related to melanogenesis...
  93. pmc The acute-phase reactant C-reactive protein binds to phosphorylcholine-expressing Neisseria meningitidis and increases uptake by human phagocytes
    Rosalyn Casey
    Division of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Stag Hill, Guildford, Surrey GU2 7XH, United Kingdom
    Infect Immun 76:1298-304. 2008
    ..has been shown to help protect the host from several bacterial pathogens, which it recognizes by binding to phosphorylcholine (PC) on their surfaces...
  94. ncbi Quantification of the response to miltefosine treatment for visceral leishmaniasis by QT-NASBA
    P J de Vries
    Academic Medical Center, Division of Infectious Diseases, Tropical Medicine and AIDS, Meibergdreef 9, Amsterdam, The Netherlands
    Trans R Soc Trop Med Hyg 100:1183-6. 2006
    ..QT-NASBA values declined exponentially and were negative after 6 weeks. Miltefosine plasma concentrations continued to accumulate during the 4 weeks of treatment. The terminal elimination half-life was 14.8 days...
  95. doi Transition from octahedral to tetrahedral geometry causes the activation or inhibition by Znf2+ of Pseudomonas aeruginosa phosphorylcholine phosphatase
    Lisandro H Otero
    Departamento de Biologia Molecular, Universidad Nacional de Rio Cuarto, 5800 Rio Cuarto, Cordoba, Argentina
    Biometals 23:307-14. 2010
    Pseudomonas aeruginosa phosphorylcholine phosphatase (PchP) catalyzes the hydrolysis of phosphorylcholine, which is produced by the action of hemolytic phospholipase C on phosphatidylcholine or sphyngomielin, to generate choline and ..
  96. ncbi Antibodies of IgM subclass to phosphorylcholine and oxidized LDL are protective factors for atherosclerosis in patients with hypertension
    Jun Su
    Center of Infectious Medicine and Unit of Rheumatology, F 59 Department of Medicine, Karolinska University Hospital, Huddinge, Stockholm 141 86, Sweden
    Atherosclerosis 188:160-6. 2006
    To determine the importance of antibodies against phosphorylcholine (PC) and oxidized low density lipoprotein (OxLDL) for development of atherosclerosis.
  97. ncbi The sensitivity of clinical isolates of Leishmania from Peru and Nepal to miltefosine
    Vanessa Yardley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Am J Trop Med Hyg 73:272-5. 2005
    ..If this model can be correlated to therapeutic outcome, it may have implications for the interpretation of clinical trials...
  98. doi Miltefosine treatment of Leishmania major infection: an observational study involving Dutch military personnel returning from northern Afghanistan
    P P A M van Thiel
    Department of Internal Medicine, Division of Infectious Diseases, Tropical Medicine and AIDS and Center for Infection and Immunity, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Clin Infect Dis 50:80-3. 2010
    ..Thirty patients experienced cure after receipt of miltefosine, 3 after receipt of additional ilSb(v), and 1 after 28 daily intravenous injections of antimony. Temporary diminution of ejaculate volume was reported by 21 patients...
  99. ncbi Efficacy and tolerability of miltefosine for childhood visceral leishmaniasis in India
    Sujit K Bhattacharya
    Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, India
    Clin Infect Dis 38:217-21. 2004
    ..This trial indicates that miltefosine is as effective and well tolerated in Indian children with VL as in adults and that it can be recommended as the first choice for treatment of childhood VL in India...
  100. pmc Noninvasive detection of lentiviral-mediated choline kinase targeting in a human breast cancer xenograft
    Balaji Krishnamachary
    Johns Hopkins University In Vivo Cellular Molecular Imaging Center Program, The Russell H Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Cancer Res 69:3464-71. 2009
    ..This study shows the use of lentiviral vectors to target chk in a human breast cancer xenograft and noninvasive MRS detection of this targeting...
  101. ncbi A new class of anticancer alkylphospholipids uses lipid rafts as membrane gateways to induce apoptosis in lymphoma cells
    Arnold H van der Luit
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Mol Cancer Ther 6:2337-45. 2007
    ..Overall, our results suggest that lipid rafts are critical membrane portals for cellular entry of alkylphospholipids depending on SMS1 activity and, therefore, are potential targets for alkylphospholipid anticancer therapy...

Research Grants66

  1. Global regulatory interactions in bacterial pathogenesis
    BRIAN AKERLEY; Fiscal Year: 2009
    ..One such structure, phosphorylcholine (PC), contributes to pathogenesis by promoting adherence to host cells, yet is also a target for host immune ..
  2. Deregulated lipid metabolism in stroke
    Robert J Dempsey; Fiscal Year: 2013
    ..This proposal explores the therapeutic potential of D609 and how it affects lipid second messenger ceramide that regulates the cell cycle both in vitro and in vivo stroke models. ..
  3. H. influenzae: Role of Sialic Acid in Virulence
    Michael Apicella; Fiscal Year: 2009
    ..from these patients now allow determination of how phase variation effects the state of sialylation and phosphorylcholine expression in NTHi after colonization of the normal human nasopharynx...
  4. PPAR delta functions in liver
    Chih Hao Lee; Fiscal Year: 2013
    ..The study is expected to lead to new areas of research and to have important clinical implication, as this novel lipid signaling may serve as a marker and a therapeutic target of metabolic diseases. ..
  5. The Role of PcpA in the Mechanism of Virulence and Protective Immunity.
    MELISSA MARIE WALKER; Fiscal Year: 2013
    ....
  6. Functional Genomics and Molecular Imaging of Liver Disease and Cancer
    GORDON SHOJI OKIMOTO; Fiscal Year: 2013
    ..This proposal is submitted in response to NIH PA-08-243. ..
  7. BIOACTIVITIES OF PNEUMOCOCCAL CELL WALL IN MENINGITIS
    Elaine I Tuomanen; Fiscal Year: 2013
    ..blood brain barrier (CbpA on the cell wall ligates laminin receptor LR) and then penetrates into the CNS (phosphorylcholine PCho on the cell wall ligates platelet activating factor receptor PAFr) and have shown that these steps are ..
  8. Quantitative Biomarker Imaging for Early Therapy Response Assessment in Cancer
    James M Mountz; Fiscal Year: 2013
    ..Development and use of quantitative imaging for early therapy assessment will greatly facilitate cancer patient management by sparing patients from weeks or months of toxicity and ineffective treatment. ..
  9. Host sphingolipids and fungal infection
    Maurizio Del Poeta; Fiscal Year: 2011
    ..The studies that we propose in this application will reveal new regulatory mechanisms involved in the killing of Cn by neutrophils. We will identify the nexus between SMS, PKD and infection. ..
  10. STRUCTURE AND FUNCTION OF PHOSPHOETHANOLAMINE METHYLTRANSFERASES: NEW ANTI-PARAS
    JOSEPH MARTIN JEZ; Fiscal Year: 2013
    ....
  11. CryoMAS Probes for Order-of-Magnitude Advance in S/N in NMR of Solids
    FRANCIS DAVID DOTY; Fiscal Year: 2011
    ..Market potential of CryoMAS probes over the 15 years following completion of the Phase II almost certainly exceeds $100M and may exceed $300M. ..
  12. Genetic Control of Phospholipid Biosynthesis and Muscular Dystrophy
    Gregory A Cox; Fiscal Year: 2013
    ....
  13. Functional Imaging of Twist induced breast cancer
    Venu Raman; Fiscal Year: 2013
    ..In the long term, these studies will help in the pathological diagnosis of breast cancer and provide insights that will contribute to the development of novel imaging markers and chemotherapeutic drugs for breast cancer. ..
  14. Selection of B cell repertoire in senescence
    Richard L Riley; Fiscal Year: 2013
    ..pneumoniae antigen phosphorylcholine will be assessed...
  15. Oxidized Phospholipid-Induced Inflammation in Atherosclerosis
    Norbert Leitinger; Fiscal Year: 2010
    ..We will investigate the chemokine expression pattern as well as time course of expression in OxPL-induced compared to LPS-induced inflammation. Furthermore, we will investigate the role of 12-LO in OxPL-induced inflammation in vivo. ..
  16. Synthesis and Application of Novel Apolipoprotein Mimetics
    M Reza Ghadiri; Fiscal Year: 2012
    ..Our results may establish a platform for potential therapeutic agents to treat atherosclerosis by the regression of atherosclerotic plaque in vivo. ..
  17. Zwitterionic Clicking for Enhanced Orthopedics
    LAURIE LAWIN; Fiscal Year: 2012
    ..Academic work from Japan demonstrated a promising photochemical graft coating of phosphorylcholine zwitterion that reduced wear significantly while also decreasing pro-inflammatory cytokine release and ..
  18. CryoMAS Probes for Order-of-Magnitude Advance in S/N in NMR of Solids
    FRANCIS DAVID DOTY; Fiscal Year: 2013
    ..Key Words: solids NMR probes, cryoprobes, HR-MAS, cancer diagnostic tests, molecular structures, cryogenic preamps, dynamic nuclear polarization (DNP) ..
  19. Development of Chemical Receptors for Proteins
    Sanku Mallik; Fiscal Year: 2005
    ..Testing of the synthetic receptors for possible applications in detection of metastatic cancer, imaging and delivery of anti- cancer drugs. ..
  20. Selective Small Molecule Membrane Transport Using Cavitand Receptors
    Michael P Schramm; Fiscal Year: 2013
    ..These new approaches to selective molecular transport will provide exciting opportunities towards advancing current drug delivery methods and designing nanoscale medical devices. ..
  21. PLATELET ACTIVATING FACTOR DEPENDENT TRANSACETYLASES
    TEN CHING LEE; Fiscal Year: 1999
    ..Obtaining such information could ultimately be used to modify some of the adverse biological consequences elicited by PAF. ..
  22. MR Metabolic Imaging of Response to Targeted Therapies in GBM
    SABRINA MIRIAM RONEN; Fiscal Year: 2013
    ..It will enable longitudinal monitoring of drug action at the tumor site and will provide a tool to optimize therapeutic regimens, resulting in more personalized care and improved outcome for GBM patients. ..
  23. CHOLINERGIC PHARMACOLOGY OF BASAL FOREBRAIN NEURONS
    Susan Robinson; Fiscal Year: 1990
    ..by a mass fragmentographic technique that measures the relative incorporation of deuterium label from phosphorylcholine into choline and ACh...
  24. A Novel Approach to the Development of a Syphilis Vaccine Using Rabbit Monoclonal
    James Miller; Fiscal Year: 2009
    ..M131 binds a phosphorylcholine epitope on the T. pallidum surface...
  25. A Phosphorylcholine Vesicle (PCV) Platform for Cancer Therapeutics
    TODD SHANNON EMRICK; Fiscal Year: 2013
    ..provided by applicant): A polymer therapeutics and nanotechnology project is proposed, in which a phosphorylcholine vesicle (PCV) platform is designed and synthesized to improve the efficacy of cancer drugs...
  26. C-Reactive Protein in Human Mucosal Immunity
    Jane Gould; Fiscal Year: 2005
    ..It's function in the serum includes binding to phosphorylcholine (ChoP) on the cell wall of Streptococcus pneumoniae, which leads to activation of the complement pathway and ..
  27. Competition Among Bacterial Resiratory Pathogens
    Jeffrey Weiser; Fiscal Year: 2004
    ..Both organisms express cell-surface phosphorylcholine (ChoP), an unusual bacterial structure that allows for binding to epithelial cells expressing the receptor ..
  28. METABOLISM OF PLATELET ACTIVATING FACTOR
    ROBERT WYKLE; Fiscal Year: 1993
    ..Effects of the diet on PAF synthesis and on responses to PAF will be investigated...
  29. Regulation of PAF Acetylhydrolase Expression by Oxidized Phospholipids
    SUZANNE BARBOUR; Fiscal Year: 2009
    ..The objective of this study is to delineate the molecular mechanisms of PAFAH induction by oxPL in hopes that this will uncover novel mechanisms for regulating the enzyme and thereby controlling cardiovascular disease. ..
  30. CELL SIGNALLING BY PHOSPHATIDYLCHOLINE BREAKDOWN
    John Exton; Fiscal Year: 1993
    ..P-choline is elevated in certain human cancer cells and in fibroblasts transformed by the Ha-ras oncogene...
  31. ANALYSIS OF IDIOTYPE MUTANTS
    Heinz Kohler; Fiscal Year: 1980
    The S107 BALB/c plasmacytoma tumor produces an IgA myeloma protein which has specificity for the hapten phosphorylcholine (PC)...
  32. NOVEL METHODS FOR PREPARING SYNTHETIC ANTIBODIES
    Sanku Mallik; Fiscal Year: 1999
    ..abstract_text> ..
  33. The Exploration of New Bio-Relevant Materials Using Surface-Initiated ROMP of Low
    Wei Chen; Fiscal Year: 2007
    ..Of the few existing biocompatible materials, poly(ethylene glycol) (PEG) and phosphorylcholine-containing molecules are the most well-recognized and studied...
  34. ACCELERATED PLASMALOGEN CATABOLISM DURING ISCHEMIA
    David Ford; Fiscal Year: 1999
    ....
  35. FUNCTIONAL DIFFERENTIATION IN B LYMPHOCYTES
    MARVIN RITTENBERG; Fiscal Year: 2002
    ..These studies bear on B-cell wastage and homeostasis and the causes of apoptosis in germinal centers where recent evidence has suggested some lymphoid tumors such as Hodgkin's disease may originate. ..
  36. MR Based Molecular Imaging of Prostate Cancer
    John Kurhanewicz; Fiscal Year: 2009
    ....
  37. REGULATION OF ENZYME OF LIPID METABOLISM
    Robert Bell; Fiscal Year: 1990
    ..Alterations of sphingolipid metabolites are likely of importance to the sphingolipidosis, progressive dementias and other neurological disorders, cancer, psoriasis, immune disorders and inflammation...
  38. Endothelial Metabolism of Atherogenic Aldehydes
    Sanjay Srivastava; Fiscal Year: 2005
    ..These studies could also form the basis of future assessments of individual risk for atherosclerosis due to differences in the vascular metabolism of aldehydes. ..
  39. Bacterial host mimicry and chronic airway infection
    WILLIAM SWORDS; Fiscal Year: 2009
    ..that share certain structural modifications, including the addition of host structures like sialic acid and phosphorylcholine. Data included in this application show that the addition of phosphorylcholine to these endotoxins decreases ..
  40. Biosynthesis of Phosphorylcholine Oligosaccharides
    CARLOS HIRSCHBERG; Fiscal Year: 2007
    ..Previous studies have suggested that the anti-inflammatory action of phosphorylcholine oligosaccharide -containing proteins, occurring in nematodes, but not in the hosts, play an important role ..
  41. Global regulatory interactions in bacterial pathogenesis
    BRIAN AKERLEY; Fiscal Year: 2006
    ..influenzae that disrupts redox control over one such modification (addition of a phosphorylcholine epitope, termed ChoP, to the LPS) and also results in a pronounced colonization defect in an animal model of ..
  42. ACID SPHINGOMYELINASE ACTIVATION IN APOPTOSIS
    Timothy Driscoll; Fiscal Year: 2000
    ..D3 and TNF-alpha activated a neutral sphingomyelinase, which hydrolyzes sphingomyelin to ceramide and phosphorylcholine. Ceramide inhibits cell proliferation, induces differentiation, and stimulates apoptosis...
  43. Bacterial phosphorycholine and pathogenesis
    Jeffrey Weiser; Fiscal Year: 2004
    We have shown that choline phosphate or phosphorylcholine (ChoP) is found on the surface of numerous bacterial species...
  44. Activation of CTP:phosphocholine cytidyltransferase
    JON FRIESEN; Fiscal Year: 2002
    ....
  45. REGULATION OF POLYPLOIDY BY CYTIDYLYLTRANSFERASE (CT)
    Zheng Cui; Fiscal Year: 2002
    ..A better understanding of the mechanism that underlines the genome instability in cancer cells will be helpful for designing more efficient strategies of treating cancer. ..
  46. Structural mimicry and host innate responses
    Shayla West Barnette; Fiscal Year: 2006
    ..Work in Dr. Swords' laboratory has established that one such epitope, phosphorylcholine, profoundly changes host-pathogen interactions for the bacterium Haemophilus influenzae...
  47. CONTROL OF ANTIBODY DIVERSITY AND EXPRESSION
    J Claflin; Fiscal Year: 1991
    ..We expect that new and significant information will also be forthcoming about the nature of B cell memory...
  48. Role of B-1 Lymphocytes in Athergenesis
    Joseph Witztum; Fiscal Year: 2004
    ..sequenced their variable region genes and discovered a 100% homology of both VH/VL gene usage with T15 anti-phosphorylcholine (PC) Abs that have been extensively studied for over 30 years. T15 Abs bind to S...
  49. B-LYMPHOCYTE MEDIATED IMMUNE MECHANISMS IN ATHEROGENESIS
    Joseph Witztum; Fiscal Year: 2001
    ..their variable region genes and discovered a 100 percent homology of both VH/VL gene usage with T15 anti-phosphorylcholine (PC) Abs that have been extensively studied for over 30 years. T15 Abs bind to S...
  50. IN VIVO 31P-1H MRSI AND MRI BRAIN STUDIES OF NICOTINE
    JAY PETTEGREW; Fiscal Year: 2004
    ..We propose to investigate these aspects of brain metabolism in humans using 31P-1H MRSI. ..
  51. High Field MRS Assessment of Stimulant Exposure in Rats
    Matthew Galloway; Fiscal Year: 2007
    ..Development of a neurochemical fingerprint describing the effects of 3 stimulants will provide a solid basis for hypothesis-driven investigations of stimulant-induced plasticity and/or toxicity in the CNS. ..
  52. ALKYL PHOSPHOCHOLINE AND PHOSPHOETHANOLIMINE DETERGENTS
    MELVIN KEYES; Fiscal Year: 1999
    ..A great need exists for new, pure structurally defined detergents for purifying and analyzing membrane proteins. Anatrace plans to market these new detergents to the research community via its established distribution routes. ..
  53. TOXINS AND HEPATOCELLULAR PHOSPHOGLYCERIDE CATABOLISM
    ROBERT LAMB; Fiscal Year: 1990
    ..These studies will increase our understanding of how reactive chemical metabolites cause liver cell injury. This information is crucial to our understanding of how to prevent and treat chemical-dependent liver cell necrosis...
  54. A Cryo-coil MAS 1H/13C/2H/15N Probe for Highest S/N in NMR of Solids
    FRANCIS DOTY; Fiscal Year: 2009
    ..This is important to drug developments, catalysts, and enzymes, to name but a few. ..
  55. REGULATION OF MAMMALIAN PHOSPHOLIPID METABOLISM
    Claudia Kent; Fiscal Year: 1991
    ..These experiments will thus examine in depth the structural aspects of cytidylyltransferase that allow it to be found associated with different cellular structures, as will identify those structures...
  56. Cryo-coil MAS H/ C/ H/ N/ Probe for Highest S/N in NMR
    FRANCIS DOTY; Fiscal Year: 2006
    ..Initial field-testing at the University of Georgia is expected midway through the Phase II. Subsequent work following this Phase II is expected to extend the technology to 800 MHz. ..
  57. IDIOTYPE RECOGNIZING T-HELPER CELLS
    Heinz Kohler; Fiscal Year: 1991
    ..is on the role of T helper cells which recognize the dominant T15 idiotype in the response of BALB/c mice to phosphorylcholine (PC)...
  58. NMR STUDIES OF BRAIN AGING IN ALZHEIMER'S DISEASE
    William Klunk; Fiscal Year: 1990
    ..These studies could provide insights into the role of brain aging on its response to brief anoxia or ischemia...
  59. IN VIVO 31P MRS STUDIES OF NON-HODGKIN'S LYMPHOMA
    Truman R Brown; Fiscal Year: 2010
    ..The proposed research is the final step leading a directed-therapy clinical trial and the culmination of an unprecedented program of translational research in MRS. ..
  60. Cryo-coil HR-MAS Probe for SNR in NMR of Tissues
    F Doty; Fiscal Year: 2002
    ..8 T (800 MHz). Initial field testing at an outside institution is expected by the end of Phase II. ..
  61. EXCESS METHYLATION & PARKINSONS DISEASE--PROPOSALS
    CLIVEL CHARLTON; Fiscal Year: 2000
    ..Lyso-PTC is cytotoxic. Free diacylglycerol (DAG), arachidonic acid (AA), phosphatidic acid and phosphorylcholine are also produced with potentially neuropathological consequences...
  62. ANTIGEN AND ANTIBODY IN THE IMMUNE RESPONSE
    Heinz Kohler; Fiscal Year: 1980
    Balb/c or A/He mice immunized with the phosphorylcholine (PC) binding IgA myeloma protein (T15), produced by the TEPC-15 myeloma, produce antibody which specifically neutralizes mouse antibody to PC and suppresses the antibody response ..
  63. THE MICROVASCULAR PATHOBIOLOGY OF AGEPC
    David Humphrey; Fiscal Year: 1990
    ..objective of this research is to characterize the biological roles of 1-0-hexadecyl-2-acetyl-sn-glyceryl-3- phosphorylcholine (AGEPC) as a mediator of inflammation...
  64. IMMUNE RESPONSE TO HAPTEN MODIFIED LYSOZYME
    Byung Kim; Fiscal Year: 1999
    ..It has been demonstrated that immunization of non/low-responder mice with phosphorylcholine (PC)-conjugated HEL (PC-HEL) can convert these low- responder mice into high-responders for antibody as well ..
  65. CDP-choline: Mechanisms in Cerebral Ischemia
    RAO ADIBHATLA; Fiscal Year: 2006
    ..Arachidonic acid activates neutral sphingomyelinase, resulting in membrane disintegration. PCCT activity was stimulated by exogenous CDP-choline. Aim 2 will be tested in gerbil transient forebrain ischemia. ..
  66. BACTERIAL PHOSPHORYLCHOLINE AND PATHOGENESIS
    Jeffrey Weiser; Fiscal Year: 2009
    b>Phosphorylcholine (ChoP) is found on the surface of many of the potentially invasive pathogens that colonize the mucosal surface of the respiratory tract, including members of the genera Streptococcus, Haemophilus, and Neisseria...