brefeldin a

Summary

Summary: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.

Top Publications

  1. doi Differential effects of the brefeldin A analogue (6R)-hydroxy-BFA in tobacco and Arabidopsis
    Markus Langhans
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, D 69120 Heidelberg, Germany
    J Exp Bot 62:2949-57. 2011
  2. ncbi Endoplasmic reticulum stress stimulates the expression of cyclooxygenase-2 through activation of NF-kappaB and pp38 mitogen-activated protein kinase
    Jui Hsiang Hung
    Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
    J Biol Chem 279:46384-92. 2004
  3. doi BFA effects are tissue and not just plant specific
    David G Robinson
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Trends Plant Sci 13:405-8. 2008
  4. ncbi Intracellular localization and in vivo trafficking of p24A and p23
    R Blum
    Institute of Physiology II, Medical Biochemistry and Department of Anatomy, University of the Saarland, D 66421 Homburg Saar, Germany
    J Cell Sci 112:537-48. 1999
  5. ncbi A GFP-based assay reveals a role for RHD3 in transport between the endoplasmic reticulum and Golgi apparatus
    Huanquan Zheng
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Plant J 37:398-414. 2004
  6. pmc The endosomal system of plants: charting new and familiar territories
    David G Robinson
    Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Plant Physiol 147:1482-92. 2008
  7. ncbi GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat
    Kazumasa Kawamoto
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Traffic 3:483-95. 2002
  8. pmc A critical role of a cellular membrane traffic protein in poliovirus RNA replication
    George A Belov
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000216. 2008
  9. pmc Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination
    O Staub
    Hospital for Sick Children, Division of Respiratory Research, Toronto, Ontario, Canada
    EMBO J 16:6325-36. 1997
  10. pmc PINOID positively regulates auxin efflux in Arabidopsis root hair cells and tobacco cells
    Sang Ho Lee
    Department of Biology, Chungnam National University, Daejeon 305 764, Korea
    Plant Cell 18:1604-16. 2006

Detail Information

Publications260 found, 100 shown here

  1. doi Differential effects of the brefeldin A analogue (6R)-hydroxy-BFA in tobacco and Arabidopsis
    Markus Langhans
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, D 69120 Heidelberg, Germany
    J Exp Bot 62:2949-57. 2011
    The effects of two brefeldin A (BFA) analogues (BFA lactam; 6(R)-hydroxy-BFA) on plant cells were tested...
  2. ncbi Endoplasmic reticulum stress stimulates the expression of cyclooxygenase-2 through activation of NF-kappaB and pp38 mitogen-activated protein kinase
    Jui Hsiang Hung
    Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
    J Biol Chem 279:46384-92. 2004
    ..Tunicamycin and brefeldin A, two ER stress inducers, increased the expression of COX-2 in ML-1 or MCF-7 cells...
  3. doi BFA effects are tissue and not just plant specific
    David G Robinson
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Trends Plant Sci 13:405-8. 2008
    b>Brefeldin A (BFA) is one of the most popular drugs used by researchers for studies on secretion and endocytosis because it interferes with specific vesicle coat proteins via action on a guanine nucleotide exchange factor...
  4. ncbi Intracellular localization and in vivo trafficking of p24A and p23
    R Blum
    Institute of Physiology II, Medical Biochemistry and Department of Anatomy, University of the Saarland, D 66421 Homburg Saar, Germany
    J Cell Sci 112:537-48. 1999
    ..This study therefore gives new insights into the localization and trafficking behavior of the two COPI-binding proteins p24A and p23...
  5. ncbi A GFP-based assay reveals a role for RHD3 in transport between the endoplasmic reticulum and Golgi apparatus
    Huanquan Zheng
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Plant J 37:398-414. 2004
    ..Inhibition of secGFP transport between the ER and plasma membrane (PM), either by Brefeldin A (BFA) treatment or by genetic intervention results in increased intracellular secGFP accumulation accompanied ..
  6. pmc The endosomal system of plants: charting new and familiar territories
    David G Robinson
    Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Plant Physiol 147:1482-92. 2008
  7. ncbi GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat
    Kazumasa Kawamoto
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Traffic 3:483-95. 2002
    ..This process is blocked by brefeldin A, which is an inhibitor of guanine nucleotide exchange factors for ADP-ribosylation factor...
  8. pmc A critical role of a cellular membrane traffic protein in poliovirus RNA replication
    George A Belov
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000216. 2008
    ..Virus replication is inhibited by brefeldin A (BFA), implicating some components(s) of the cellular secretory pathway in virus growth...
  9. pmc Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination
    O Staub
    Hospital for Sick Children, Division of Respiratory Research, Toronto, Ontario, Canada
    EMBO J 16:6325-36. 1997
    ..retention or recycling of ENaC and incorporation of new channels at the plasma membrane, as determined by Brefeldin A treatment...
  10. pmc PINOID positively regulates auxin efflux in Arabidopsis root hair cells and tobacco cells
    Sang Ho Lee
    Department of Biology, Chungnam National University, Daejeon 305 764, Korea
    Plant Cell 18:1604-16. 2006
    ..Localization of PID or PIN3 at the cell boundary was disrupted by brefeldin A and staurosporine...
  11. ncbi Munc18 interacting proteins: ADP-ribosylation factor-dependent coat proteins that regulate the traffic of beta-Alzheimer's precursor protein
    Karen Hill
    Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322 3050, USA
    J Biol Chem 278:36032-40. 2003
    ..We propose that MINTs are a family of Arf-dependent, vesicle-coat proteins that can regulate the traffic of beta-APP...
  12. pmc Caveolar endocytosis of simian virus 40 is followed by brefeldin A-sensitive transport to the endoplasmic reticulum, where the virus disassembles
    Leonard C Norkin
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003 5720, USA
    J Virol 76:5156-66. 2002
    ..Additionally, transport of SV40 to the ER, as well as infection, is sensitive to brefeldin A. This drug acts by specifically inhibiting the ARF1 GTPase, which is known to regulate assembly of COPI coat ..
  13. ncbi The antibody to GD3 ganglioside, R24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment. Inhibitory effect of brefeldin A and monensin
    Ramiro Iglesias-Bartolomé
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Universidad Nacional de Cordoba, Argentina
    FEBS J 273:1744-58. 2006
    ..exits the recycling compartment en route to the plasma membrane by a dynamin 2-dependent pathway sensitive to brefeldin A and monensin...
  14. ncbi Involvement of caspase-2 and caspase-9 in endoplasmic reticulum stress-induced apoptosis: a role for the IAPs
    Herman H Cheung
    Apoptosis Research Centre, Children s Hospital of Eastern Ontario Research Institute II, Ottawa, Ontario, Canada K1H 8L1
    Exp Cell Res 312:2347-57. 2006
    ..Here we show that brefeldin A and tunicamycin-mediated ER stress lead to caspase-dependent apoptosis involving caspase-2...
  15. ncbi ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1
    S E Langille
    Program in Molecular Medicine, Worcester, Massachusetts 01605, USA
    J Biol Chem 274:27099-104. 1999
    ..GTP loading of ARF6 in both control cells and in response to GRP1 or cytohesin-1 was insensitive to brefeldin A, consistent with previous data on endogenous ARF6 exchange activity...
  16. pmc PDMP blocks brefeldin A-induced retrograde membrane transport from golgi to ER: evidence for involvement of calcium homeostasis and dissociation from sphingolipid metabolism
    J W Kok
    Department of Physiological Chemistry, University of Groningen, Groningen Institute for Drug Studies GIDS, 9713 AV Groningen, The Netherlands
    J Cell Biol 142:25-38. 1998
    ..of sphingolipid biosynthesis, D,L-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol (PDMP), inhibits brefeldin A (BFA)-induced retrograde membrane transport from Golgi to endoplasmic reticulum (ER)...
  17. ncbi On the action of Brefeldin A on Sec7-stimulated membrane-recruitment and GDP/GTP exchange of Arf proteins
    J Cherfils
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Biochem Soc Trans 33:635-8. 2005
    ..The drug BFA (Brefeldin A) was used to trap the complex that initiates the exchange reaction, providing a structural basis for its ..
  18. ncbi Transmembrane domain length determines intracellular membrane compartment localization of syntaxins 3, 4, and 5
    R T Watson
    Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA
    Am J Physiol Cell Physiol 281:C215-23. 2001
    ..These data support a model wherein short transmembrane domains (< or =17 amino acids) direct the cis-Golgi localization of syntaxins, whereas long transmembrane domains (> or =23 amino acids) direct plasma membrane localization...
  19. doi The SPCA1 Ca2+ pump and intracellular membrane trafficking
    Massimo Micaroni
    Laboratory of Intracellular Traffic, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Via Nazionale 8 A, 66030 Santa Maria Imbaro Chieti, Italy
    Traffic 11:1315-33. 2010
    ..and delays retrograde redistribution of the GA glycosylation enzymes into the endoplasmic reticulum caused by brefeldin A; however, exit of these enzymes from the endoplasmic reticulum is not affected...
  20. pmc Characterization of class I and II ADP-ribosylation factors (Arfs) in live cells: GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1
    Justin Chun
    Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Mol Biol Cell 19:3488-500. 2008
    ..Live cell imaging detected Arfs on peripheral puncta that also contained Golgi-specific brefeldin A (BFA) resistance factor (GBF) 1 and the ERGIC marker p58...
  21. pmc Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain
    N Morinaga
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:12856-60. 1996
    b>Brefeldin A (BFA) inhibited the exchange of ADP ribosylation factor (ARF)-bound GDP for GTP by a Golgi-associated guanine nucleotide-exchange protein (GEP) [Helms, J.B. & Rothman, J.E...
  22. ncbi Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation
    Chisa Shinotsuka
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biol Chem 277:9468-73. 2002
    ..A fungal metabolite, brefeldin A (BFA), inhibits ARF-GEFs and leads to redistribution of coat proteins from membranes to the cytoplasm and ..
  23. pmc Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells
    Yu Chung Tse
    Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant Cell 16:672-93. 2004
    ..b>Brefeldin A induced the YFP-labeled Golgi stacks but not the YFP-marked PVCs to form typical enlarged structures...
  24. ncbi Functional diversification of closely related ARF-GEFs in protein secretion and recycling
    Sandra Richter
    ZMBP, Entwicklungsgenetik, Tubingen, Germany
    Nature 448:488-92. 2007
    ....
  25. pmc Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets
    A G Ostermeyer
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Cell Biol 152:1071-8. 2001
    ..Third, incubation of cells with brefeldin A caused caveolin-1 to accumulate in the droplets...
  26. doi Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuole
    Ombretta Foresti
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
    Mol Plant 1:1067-76. 2008
    ..is delivered to the vacuole and fragmented, in a process that is inhibited by the secretory traffic inhibitor brefeldin A. Moreover, a fusion between GFP and a domain of the maize storage protein gamma-zein involved in zein ..
  27. pmc ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes
    C E Ooi
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    J Cell Biol 142:391-402. 1998
    ..Association of AP-3 with membranes in vitro was enhanced by GTPgammaS and inhibited by brefeldin A (BFA), an inhibitor of ARF1 guanine nucleotide exchange...
  28. ncbi The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
    Niko Geldner
    ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
    Cell 112:219-30. 2003
    ..The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of ..
  29. pmc p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A
    S J Mansour
    Department of Cell Biology, University of Alberta, MSB 5 14, Edmonton, AB T6G 2H7, Canada
    Proc Natl Acad Sci U S A 96:7968-73. 1999
    The drug brefeldin A (BFA) disrupts protein traffic and Golgi morphology by blocking activation of ADP ribosylation factors (ARFs) through an unknown mechanism...
  30. ncbi Brefeldin A action and recovery in Chlamydomonas are rapid and involve fusion and fission of Golgi cisternae
    E Hummel
    Department of Cell Biology, Heidelberg Institute for Plant Sciences HIP, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
    Plant Biol (Stuttg) 9:489-501. 2007
    ..These structures then divide by vertical fission to produce Golgi stacks of normal size and morphology roughly 60 min after drug wash-out...
  31. pmc Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway
    Claude Saint-Jore-Dupas
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant Cell 18:3182-200. 2006
    ..We conclude that the localization of N-glycan processing enzymes corresponds to an assembly line in the early secretory pathway and depends on both TMD length and signals in the cytoplasmic tail...
  32. pmc GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 149:81-94. 2000
    ..Treatment with brefeldin A or overexpression of dominant-negative ADP ribosylation factor 1 (ARF1) caused dissociation of GGAs from ..
  33. ncbi Brefeldin a inhibits circadian remodeling of chloroplast structure in the dinoflagellate gonyaulax
    Nasha Nassoury
    Institut de recherche en biologie végétale, Departement de sciences biologiques, Universite de Montreal, 4101 Sherbrooke est, Montreal, Quebec, Canada H1X2B2
    Traffic 6:548-61. 2005
    ..Rubisco transit through the Golgi in dinoflagellates; hence, we blocked protein import into the plastids using Brefeldin A (BFA) to explore the mechanism for plastid remodeling...
  34. ncbi The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast
    A Peyroche
    Service de Biochimie et Génétique Moléculaire, Bat 142, Departement de Biologie Cellulaire et Moleculaire, CEA Saclay, 91191 Gif sur Yvette, France
    J Cell Sci 114:2241-53. 2001
    ..In addition, the structure of Golgi elements was severely perturbed in gea mutants. We conclude that Gea1p and Gea2p play an important role in the structure and functioning of the Golgi apparatus in yeast...
  35. doi Proteomics on brefeldin A-treated Arabidopsis roots reveals profilin 2 as a new protein involved in the cross-talk between vesicular trafficking and the actin cytoskeleton
    Tomáš Takáč
    Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Department of Cell Biology, Palacký University, Olomouc, Czech Republic
    J Proteome Res 10:488-501. 2011
    ..Here, proteomic and cell biology approaches were applied to study effects of brefeldin A (BFA), an inhibitor of vesicle recycling and secretion, in Arabidopsis roots...
  36. pmc Quantitative ER <--> Golgi transport kinetics and protein separation upon Golgi exit revealed by vesicular integral membrane protein 36 dynamics in live cells
    T Dahm
    Light Microscopy Group, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Biol Cell 12:1481-98. 2001
    ....
  37. ncbi Auxin transport inhibitors block PIN1 cycling and vesicle trafficking
    N Geldner
    , , Auf der Morgenstelle 3, , Germany
    Nature 413:425-8. 2001
    ..In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.
  38. ncbi Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A
    Elena Ortiz-Zapater
    Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad de Valencia, Avda Vicente Andres Estelles s n, 46100 Burjassot Valencia, Spain
    Plant J 48:757-70. 2006
    ..In contrast, treatment with brefeldin A, which inhibits recycling from endosomes back to the plasma membrane in plant cells, leads to the accumulation ..
  39. ncbi Structural integrity of the Golgi stack is essential for normal secretory functions of rat parotid acinar cells: effects of brefeldin A and okadaic acid
    Hideaki Tamaki
    Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
    J Histochem Cytochem 50:1611-23. 2002
    We examined the effects of specific inhibitors, brefeldin A (BFA) and okadaic acid (OA), on the ultrastructural organization of the Golgi apparatus and distributions of amylase, Golgi-associated proteins, and cathepsin D in the rat ..
  40. ncbi Inhibition of Japanese encephalitis virus maturation and transport in PS cells to cell surface by brefeldin A
    V J Lad
    National Institute of Virology, Indian Council of Medical Research, 20 A, Dr Ambedkar Road, P B No 11, Pune 411 001, India
    Acta Virol 46:187-90. 2002
    A brefeldin A (BFA) treatment of porcine stable kidney (PS) cells resulted in inhibition of Japanese encephalitis virus (JEV) maturation and its transport to the cell surface...
  41. pmc Synaptic vesicle recycling at CNS snapses without AP-2
    Sung Hyun Kim
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA
    J Neurosci 29:3865-74. 2009
    ..These results demonstrate that in the absence of AP-2, SV proteins still become endocytosed, and synaptic vesicle recycling remains operational...
  42. ncbi Brefeldin A and filipin distinguish two globotriaosyl ceramide/verotoxin-1 intracellular trafficking pathways involved in Vero cell cytotoxicity
    Aye Aye Khine
    Department of Laboratory Medicine and Pathobiology, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada
    Glycobiology 14:701-12. 2004
    ..b>Brefeldin A (BFA), which prevents Golgi-dependent retrograde traffic, protects cells from low VT1 concentrations but not ..
  43. pmc TNO1 is involved in salt tolerance and vacuolar trafficking in Arabidopsis
    Sang Jin Kim
    Department of Genetics, Iowa State University, Ames, Iowa 50011, USA
    Plant Physiol 156:514-26. 2011
    ..The tno1 mutant had delayed formation of the brefeldin A (BFA) compartment in cotyledons upon application of BFA, suggesting less efficient membrane fusion processes in ..
  44. ncbi Ganglioside GD3 traffics from the trans-Golgi network to plasma membrane by a Rab11-independent and brefeldin A-insensitive exocytic pathway
    Pilar Maria Crespo
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, 5000 Cordoba, Argentina
    J Biol Chem 279:47610-8. 2004
    ..did not partition into GEM domains in the Golgi complex and trafficked from TGN to plasma membrane by a brefeldin A-insensitive exocytic pathway...
  45. pmc Arf3 is activated uniquely at the trans-Golgi network by brefeldin A-inhibited guanine nucleotide exchange factors
    Florin Manolea
    Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
    Mol Biol Cell 21:1836-49. 2010
    ..These results suggest that Arf3 plays a unique function at the TGN that likely involves recruitment by a specific receptor...
  46. pmc Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis
    Rodolfo D Vicetti Miguel
    Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA
    J Immunol Methods 384:191-5. 2012
    ..trachomatis genital tract infection were used to compare the ability of brefeldin A (BFA) and monensin (MN) to concurrently trap interferon-γ (IFN-γ), tumor necrosis factor (TNF), interleukin (..
  47. pmc Brefeldin A: deciphering an enigmatic inhibitor of secretion
    Andreas Nebenführ
    Department of Botany, University of Tennessee, Knoxville, Tennessee 37996 1100, USA
    Plant Physiol 130:1102-8. 2002
  48. pmc bfr1+, a novel gene of Schizosaccharomyces pombe which confers brefeldin A resistance, is structurally related to the ATP-binding cassette superfamily
    K Nagao
    Department of Biotechnology, University of Tokyo, Japan
    J Bacteriol 177:1536-43. 1995
    ..a Schizosaccharomyces pombe gene, bfr1+, which on a multicopy plasmid vector, pDB248', confers resistance to brefeldin A (BFA), an inhibitor of intracellular protein transport...
  49. pmc Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera
    Christophe Ritzenthaler
    Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
    Plant Cell 14:237-61. 2002
    b>Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus...
  50. ncbi Dissection of brefeldin A-sensitive and -insensitive steps in apicoplast protein targeting
    Amy DeRocher
    Seattle Biomedical Research Institute, 307 Westlake Avenue N, Suite 500 Seattle, WA 98109, USA
    J Cell Sci 118:565-74. 2005
    ..Incubation with brefeldin A, which blocks ER-to-Golgi trafficking by inhibiting a GTP exchange factor required for retrograde trafficking, ..
  51. ncbi Multidrug resistance phenotype conferred by overexpressing bfr2+/pad1+/sks1+ or pap1+ genes and mediated by bfr1+ gene product, a structural and functional homologue of P-glycoprotein in Schizosaccharomyces pombe
    M Arioka
    Department of Biotechnology, University of Tokyo, Japan
    Biosci Biotechnol Biochem 62:390-2. 1998
    ..These findings suggest that bfr1+ acts as the most downstream effector of the multidrug resistance conferred by bfr2+ and pap1+ genes...
  52. pmc Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor
    E Meacci
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Proc Natl Acad Sci U S A 94:1745-8. 1997
    ..Cytohesin-1 also increased ARF1 binding to a Golgi fraction, but its effect was not inhibited by brefeldin A (BFA), a drug that reversibly inhibits Golgi function...
  53. ncbi Brefeldin A influences the cell surface abundance and intracellular pools of low and high ouabain affinity Na+, K(+)-ATPase alpha subunit isoforms in articular chondrocytes
    A Mobasheri
    Department of Biomedical Sciences, School of Biosciences, University of Westminster, London, United Kingdom
    Histol Histopathol 14:427-38. 1999
    ..05) upregulation of Na+, K(+)-ATPase density and treatment with various pharmacological inhibitors (Brefeldin A, monensin and cycloheximide) significantly (p < 0.001) blocked the upregulation...
  54. ncbi Plasmodium vivax and Plasmodium chabaudi: intraerythrocytic traffic of antigenically homologous proteins involves a brefeldin A-sensitive secretory pathway
    C Bracho
    Instituto Venezolano de Investigaciones Cientificas IVIC, Caracas, Venezula
    Eur J Cell Biol 80:164-70. 2001
    ..In the presence of brefeldin A (BFA), secretion of antigenically homologous Pc-190 into the host cell cytoplasm was inhibited and the antigen ..
  55. doi Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens
    Fang yi Cheng
    Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695 7609, USA
    Planta 230:1093-103. 2009
    ..After SA treatment, MTD was not detected in the Golgi apparatus, and its SA-induced secretion was resistant to brefeldin A, an inhibitor of Golgi-mediated protein transport...
  56. doi Brefeldin A inhibits vesicular MHC class I processing in resting but not in CpG- and disruption-activated DC
    Liying Chen
    Microbiology, Tumor and Cell Biology MTC, Karolinska Institute, 171 77 Stockholm, Sweden
    Mol Immunol 46:158-65. 2008
    ..Here we investigate the sensitivity of this VP to Brefeldin A (BFA), a fungal metabolite which inhibits vesicular trafficking by preventing the activation of some ADP-..
  57. pmc Low-level doxorubicin resistance in P-glycoprotein-negative human pancreatic tumour PSN1/ADR cells implicates a brefeldin A-sensitive mechanism of drug extrusion
    V N Verovski
    Cancer Research Unit, Department of Radiotherapy, Academic Hospital, Free University Brussels, Belgium
    Br J Cancer 73:596-602. 1996
    ..In addition, these defects were reversible by the vesicular traffic inhibitors brefeldin A, fluoroaluminate and nocodazole...
  58. ncbi The role of de novo ceramide synthesis in the mechanism of action of the tricyclic xanthate D609
    Ryan J Perry
    Department of Pediatrics, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia, Canada
    J Lipid Res 45:164-73. 2004
    ..In D609-treated CHO-K1 cells, sphingomyelin synthesis was stimulated by brefeldin A, and C5-DMB-ceramide transport to the Golgi apparatus was blocked, indicating ceramide accumulation in the ..
  59. doi Different effects of aluminum on the actin cytoskeleton and brefeldin A-sensitive vesicle recycling in root apex cells of two maize varieties differing in root elongation rate and aluminum tolerance
    Montse Amenós
    Institut für Zelluläre und Molekulare Botanik, Universitat Bonn, Kirschallee 1, D 53115 Bonn, Germany
    Plant Cell Physiol 50:528-40. 2009
    ..Visualization of boron-cross-linked rhamnogalacturonan II (RGII)-containing brefeldin A (BFA) compartments revealed that Al inhibited the formation of these compartments, especially in variety 16x36...
  60. ncbi Brefeldin A disrupts asymmetric spindle positioning in mouse oocytes
    Ling Wang
    Ottawa Health Research Institute, Ottawa Hospital, Civic Campus, 1053 Carling Avenue, Ottawa, Canada
    Dev Biol 313:155-66. 2008
    ..We show here that treating denuded mouse oocytes with brefeldin A, an inhibitor of Golgi-based membrane fusion, abolished the asymmetric positioning of the metaphase I spindle ..
  61. ncbi Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1
    Melanie L Styers
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, GA 30322, USA
    J Cell Sci 119:3643-54. 2006
    ..We find that treatment of cells with Brefeldin A, an inhibitor of specific stages of membrane transport, causes changes in the organization of vimentin ..
  62. pmc Assembly and polarized release of Punta Toro virus and effects of brefeldin A
    S Y Chen
    Department of Microbiology, University of Alabama, Birmingham 35294
    J Virol 65:1427-39. 1991
    ..We have examined PTV glycoprotein transport, assembly, and release and the effects of brefeldin A (BFA) on these processes...
  63. ncbi Brefeldin A enhances Helicobacter pylori vacuolating cytotoxin-induced vacuolation of epithelial cells
    Richard H Argent
    Institute of Infection, Immunity and Inflammation, and the Wolfson Digestive Diseases Centre, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, UK
    FEMS Microbiol Lett 237:163-70. 2004
    ..We show that the Golgi-disturbing agent brefeldin A (BFA) enhances VacA-induced vacuolation of epithelial cells by Helicobacter pylori co-culture and, importantly, ..
  64. ncbi Paired basic/Furin-like proprotein convertase cleavage of Pro-BMP-1 in the trans-Golgi network
    Mat Leighton
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, Stopford Building 2 205, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 278:18478-84. 2003
    ..The observation that BMP-1 occurs inside the cell raises the possibility that BMP-1 might begin to cleave its substrates prior to secretion to the extracellular matrix...
  65. pmc ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis
    Mi Hee Lee
    Center for Plant Intracellular Trafficking, Pohang University of Science and Technology, Pohang, 790 784, Korea
    Plant Physiol 129:1507-20. 2002
    ..The treatment of protoplasts with brefeldin A had exactly the same effect as Arf1[T31N] on various intracellular trafficking pathways...
  66. ncbi Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistance
    T G Turi
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510
    J Biol Chem 269:24229-36. 1994
    The fungal metabolite brefeldin A (BFA) causes the inhibition of protein secretion and the disruption of the structure and function of the Golgi complex in mammalian cells...
  67. ncbi Molecular cloning and functional characterization of brefeldin A-ADP-ribosylated substrate. A novel protein involved in the maintenance of the Golgi structure
    S Spano
    Istituto di Ricerche Farmacologiche Mario Negri, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro Chieti, 20157 Milano, Italy
    J Biol Chem 274:17705-10. 1999
    b>Brefeldin A (BFA) is a fungal metabolite that disassembles the Golgi apparatus into tubular networks and causes the dissociation of coatomer proteins from Golgi membranes...
  68. ncbi Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane
    M M Cavenagh
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322 3050, USA
    J Biol Chem 271:21767-74. 1996
    ..The plasma membrane distrubution was unaffected by either GTPgammaS (guanosine 5'-O-(3-thio)triphosphate) or brefeldin A, an activator and inhibitor of Arf activities, respectively...
  69. ncbi Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis
    Pankaj Dhonukshe
    Section of Molecular Cytology and Center for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands
    Dev Cell 10:137-50. 2006
    ..Our results reveal that endocytic delivery of cell surface material significantly contributes to cell plate formation during plant cytokinesis...
  70. pmc Dynamic response of prevacuolar compartments to brefeldin a in plant cells
    Yu Chung Tse
    Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant Physiol 142:1442-59. 2006
    ..Here, we demonstrate that Golgi and PVCs have different sensitivity in response to brefeldin A (BFA) treatment in living tobacco BY-2 cells...
  71. pmc Uptake of a fluorescent marker in plant cells is sensitive to brefeldin A and wortmannin
    Neil Emans
    Institute for Molecular Biotechnology, Biology VII, Rheinisch Westfälische Technische Hochschule Aachen, Worringerweg 1, D 52074 Aachen, Germany
    Plant Cell 14:71-86. 2002
    ..FM1-43 uptake in suspension cells was stimulated more than twofold by brefeldin A and inhibited approximately 0.4-fold by wortmannin...
  72. ncbi Cutting edge: re-evaluating the in vivo cytokine responses of CD8+ T cells during primary and secondary viral infections
    Fei Liu
    Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 174:5936-40. 2005
    ..This technique will be useful when evaluating in vivo immune cell activity in many situations, including a variety of noninfectious (e.g., autoimmune) diseases...
  73. ncbi Ca2+ permeability of the plasma membrane induced by rotavirus infection in cultured cells is inhibited by tunicamycin and brefeldin A
    Marie Christine Ruiz
    Laboratorio de Fisiologia Gastrointestinal, Instituto Venezolano de Investigaciones Cientificas, Caracas 1020A, Venezuela
    Virology 333:54-65. 2005
    ..The viral product responsible for this effect is not known. We have used tunicamycin and brefeldin A to prevent glycosylation and membrane traffic and study the involvement of viral glycoproteins, NSP4 and/or VP7,..
  74. doi Brefeldin A activates CHOP promoter at the AARE, ERSE and AP-1 elements
    Simon C M Kwok
    Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, Philadelphia, PA 19141 3098, USA
    Mol Cell Biochem 319:203-8. 2008
    b>Brefeldin A induces apoptosis in PC-3 and MCF-7 cells at a concentration of 30 ng/ml. RT-PCR analyses showed up-regulation of CHOP/GADD153 and splicing of XBP-1 mRNA in brefeldin A-treated cells...
  75. ncbi Disturbance of endomembrane trafficking by brefeldin A and calyculin A reorganizes the actin cytoskeleton of Lilium longiflorum pollen tubes
    K Hörmanseder
    Fachbereich Molekulare Biologie, Universitat Salzburg, Hellbrunnerstrasse, Salzburg, Austria
    Protoplasma 227:25-36. 2005
    We investigated the effect of brefeldin A on membrane trafficking and the actin cytoskeleton of pollen tubes of Lilium longiflorum with fluorescent dyes, inhibitor experiments, and confocal laser scanning microscopy...
  76. doi Aggregation of proteins having Golgi apparatus sorting determinant induces large globular structures derived from the endoplasmic reticulum in plant seed cells
    Nobuyuki Maruyama
    Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    FEBS Lett 582:1599-606. 2008
    ..These results indicate that aggregation of the Golgi protein blocks transport from the ER to the Golgi...
  77. ncbi Cryopreparation provides new insight into the effects of brefeldin A on the structure of the HepG2 Golgi apparatus
    M W Hess
    Institute of Biotechnology, University of Helsinki, Helsinki, Finland
    J Struct Biol 130:63-72. 2000
    High-pressure freezing and freeze-substitution were used to study Golgi ultrastructure and its brefeldin A-induced transformations in HepG2 human hepatoma cells...
  78. pmc Capacity of the Golgi apparatus for cargo transport prior to complete assembly
    Shu Jiang
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Mol Biol Cell 17:4105-17. 2006
    ..In the case of de novo Golgi formation from the ER after brefeldin A/H89 washout in HeLa cells, we found that scattered, golgin-enriched, structures formed early and contained ..
  79. pmc Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70
    Kai feng Xu
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:2784-9. 2005
    ..Mammalian brefeldin A-inhibited guanine nucleotide-exchange proteins, BIG1 and BIG2, are important activators of ADP-ribosylation ..
  80. pmc Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi
    Ting Kuang Niu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:1213-22. 2005
    ..The drug brefeldin A (BFA), an uncompetitive inhibitor of the exchange reaction that binds to an Arf-GDP-Arf GEF complex, stabilizes ..
  81. doi Mechanosensitive hyaluronan secretion: stimulus-response curves and role of transcription-translation-translocation in rabbit joints
    A K T Wann
    Physiology, Basic Medical Sciences, St George s Hospital Medical School, University of London, London SW170RE, UK
    Exp Physiol 94:350-61. 2009
    ..the transcription-translation inhibitors actinomycin D and puromycin and by the Golgi translocation inhibitor brefeldin A. There is thus a graded coupling between HA secretion and cyclic joint movement that depends partly on new ..
  82. pmc Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors
    Philip Ian Padilla
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:2752-7. 2004
    b>Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) is an approximately 200-kDa brefeldin A-inhibited guanine nucleotide-exchange protein that preferentially activates ADP-ribosylation factor 1 (ARF1) and ARF3...
  83. ncbi Induction of terminal differentiation and apoptosis in human colonic carcinoma cells by brefeldin A, a drug affecting ganglioside biosynthesis
    H Nojiri
    Faculty of Pharmaceutical Sciences, Teikyo University, Tsukui gun, Kanagawa, Japan
    FEBS Lett 453:140-4. 1999
    ..When the cells were treated with brefeldin A (BFA), a striking increase in de novo biosynthesis of G(M3) and a decrease in biosynthesis of neolactoseries ..
  84. ncbi Sulfation in the Golgi lumen of Madin-Darby canine kidney cells is inhibited by brefeldin A and depends on a factor present in the cytoplasm and on Golgi membranes
    Katja Fjeldstad
    Department of Biochemistry and Institute for Nutrition Research, University of Oslo, Oslo 0316, Norway
    J Biol Chem 277:36272-9. 2002
    Madin-Darby canine kidney cells are more resistant than most other cell types to the classical effects of brefeldin A (BFA) treatment, the induction of retrograde transport of Golgi cisternae components to the endoplasmic reticulum...
  85. ncbi Redistribution of membrane proteins between the Golgi apparatus and endoplasmic reticulum in plants is reversible and not dependent on cytoskeletal networks
    Claude M Saint-Jore
    School of Biological and Molecular Sciences, Oxford Brookes University, Headington, Oxford OX3 0BP, UK
    Plant J 29:661-78. 2002
    ..Exposure of tissue to brefeldin A (BFA) resulted in the reversible redistribution of ST-GFP into the endoplasmic reticulum...
  86. ncbi Release of canine parvovirus from endocytic vesicles
    Sanna Suikkanen
    Department of Biological and Environmental Science, PO Box 35, FIN 40014 University of Jyväskylä, Finland
    Virology 316:267-80. 2003
    ..The presence of drugs modifying endocytosis (amiloride, bafilomycin A(1), brefeldin A, and monensin) caused viral proteins to remain in endosomal/lysosomal vesicles, even though the drugs were not ..
  87. ncbi Arabidopsis sterol endocytosis involves actin-mediated trafficking via ARA6-positive early endosomes
    Markus Grebe
    Department of Molecular Cell Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Curr Biol 13:1378-87. 2003
    ..The vesicle trafficking inhibitor brefeldin A (BFA) has been suggested to disrupt biosynthetic sterol transport at the Golgi level.
  88. ncbi Brefeldin A-regulated retrograde transport into the endoplasmic reticulum of internalised wheat germ agglutinin
    Monika Vetterlein
    Institute of Histology and Embryology, Department of Cell Biology and Ultrastructure Research, University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria
    Histochem Cell Biol 120:121-8. 2003
    The effects of the fungal metabolite brefeldin A (BFA) on the endocytic routes of internalised wheat germ agglutinin (WGA) were studied in human HepG2 hepatoma cells, drawing particular attention to the application times in relation to ..
  89. pmc Exo1: a new chemical inhibitor of the exocytic pathway
    Yan Feng
    Institute of Chemistry and Cell Biology, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6469-74. 2003
    ..Like Brefeldin A (BFA), Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less ..
  90. pmc Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors
    M Sata
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 96:2752-7. 1999
    ..fall into two classes that differ in molecular size and susceptibility to inhibition by the fungal metabolite brefeldin A (BFA)...
  91. ncbi Effects of brefeldin A on autophagy in cultured rat fibroblasts
    P Purhonen
    Department of Cell Biology, University of Jyvaskyla, Finland
    Eur J Cell Biol 74:63-7. 1997
    Effects of brefeldin A on cellular autophagy were studied in cultured rat fibroblasts...
  92. ncbi Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: involvement of specific residues of the Sec7 domain
    A Peyroche
    Service de Biochimie et Génétique Moléculaire, CEA Saclay, Gif sur Yvette, France
    Mol Cell 3:275-85. 1999
    We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and ..
  93. pmc Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking
    M Steegmaier
    Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305 5345, USA
    Mol Biol Cell 10:1957-72. 1999
    ..Immunoprecipitation of VAMP4 from rat brain detergent extracts revealed that VAMP4 exists in a complex containing syntaxin 6. Converging lines of evidence implicate a role for VAMP4 in TGN-to-endosome transport...
  94. ncbi Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46
    Lionel Breuza
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    J Biol Chem 279:47242-53. 2004
    ..we have developed a novel procedure for the purification of ERGIC membranes from HepG2 cells treated with brefeldin A, a drug known to accumulate cycling proteins in the ERGIC...
  95. ncbi Resistance against ricin-induced apoptosis in a brefeldin A-resistant mutant cell line (BER-40) of Vero cells
    Tadashi Tamura
    Division of Biochemistry, Faculty of Fisheries, Nagasaki University, Bunkyo Machi, Nagasaki, 852 8521, Japan
    J Biochem 132:441-9. 2002
    We have found that a brefeldin A (BFA)-resistant mutant cell line derived from Vero cells (BER-40) is highly resistant to ricin-induced apoptosis as compared with parental Vero cells...
  96. ncbi Synthesis and anticancer activity of brefeldin A ester derivatives
    Nwanne O Anadu
    Department of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmaceutical Sciences, and the Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
    J Med Chem 49:3897-905. 2006
    Ester derivatives of brefeldin A (BFA) were synthesized to determine which of its two hydroxyl groups could be modified while still maintaining biological activity...
  97. ncbi Dissection of membrane dynamics of the ARF-guanine nucleotide exchange factor GBF1
    Tomasz Szul
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35924, USA
    Traffic 6:374-85. 2005
    ..of an inactive (E794K) GBF1 mutant, expression of the ARF1 (T31N) mutant with decreased affinity for GTP and Brefeldin A treatment], we show that GBF1 is stabilized on membranes when in a complex with ARF-GDP...
  98. ncbi A homologue of N-ethylmaleimide-sensitive factor in the malaria parasite Plasmodium falciparum is exported and localized in vesicular structures in the cytoplasm of infected erythrocytes in the brefeldin A-sensitive pathway
    M Hayashi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan
    J Biol Chem 276:15249-55. 2001
    ..The export of PfNSF is inhibited by brefeldin A, with 9 microM causing 50% inhibition...
  99. pmc A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance
    A Pol
    Institute for Molecular Bioscience, Centre for Microscopy and Microanalysis and Department of Physiology and Pharmacology, University of Queensland, Queensland 4072, Australia
    J Cell Biol 152:1057-70. 2001
    ..We conclude that caveolin can associate with the membrane surrounding lipid droplets and is a key component involved in intracellular cholesterol balance and lipid transport in fibroblasts...
  100. ncbi Arf, Sec7 and Brefeldin A: a model towards the therapeutic inhibition of guanine nucleotide-exchange factors
    M Zeghouf
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Biochem Soc Trans 33:1265-8. 2005
    ..One of the Arf-Sec7 intermediates was trapped by BFA (Brefeldin A), an uncompetitive inhibitor of Arf activation that has been instrumental in deciphering the molecular ..
  101. ncbi Actin-based motility of endosomes is linked to the polar tip growth of root hairs
    Boris Voigt
    Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D 53115 Bonn, Germany
    Eur J Cell Biol 84:609-21. 2005
    ..In conclusion, actin-propelled endosomal motility is tightly linked to the polar tip growth of root hairs...

Research Grants67

  1. Pathogenesis of Lyme Borreliosis in the Rhesus Monkey
    MARIO PHILIPP; Fiscal Year: 2007
    ..be stimulated ex vivo with lipoprotein or live spirochetes in the presence of the protein-secretion blocker Brefeldin A. After fixation and cutting of frozen sections, the cytokine/mediator-producing cell phenotype will be ..
  2. FUNGAL POLYKETIDE SYNTHASES
    CHARLES SIH; Fiscal Year: 2001
    ..of the fungal PKSs involved in the biosynthesis of the biologically active compounds mevinolin (lovastatin) and brefeldin A. We will strive to establish how these two multifunctional proteins, which are likely to contain sets of ..
  3. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek Rangnekar; Fiscal Year: 2009
    ..The apoptotic activity was diminished in the CM from Par-4 transfectants treated with brefeldin A, indicating Par-4 and SAC secretion (or sPar-4 and sSAC, respectively) occurs by the classical pathway (i.e...
  4. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek Rangnekar; Fiscal Year: 2009
    ..The apoptotic activity was diminished in the CM from Par-4 transfectants treated with brefeldin A, indicating Par-4 and SAC secretion (or sPar-4 and sSAC, respectively) occurs by the classical pathway (i.e...
  5. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek M Rangnekar; Fiscal Year: 2010
    ..The apoptotic activity was diminished in the CM from Par-4 transfectants treated with brefeldin A, indicating Par-4 and SAC secretion (or sPar-4 and sSAC, respectively) occurs by the classical pathway (i.e...
  6. Lyme neuroborreliosis pathogenesis in the rhesus monkey
    MARIO PHILIPP; Fiscal Year: 2009
    ..are stained intracellularly with appropriate antibodies in freshly fixed brain slices treated ex vivo with Brefeldin A. The cell phenotype also will be identified, TLR expression determined, and glial/neuronal apoptosis verified ..
  7. Lyme neuroborreliosis pathogenesis in the rhesus monkey
    MARIO PHILIPP; Fiscal Year: 2007
    ..are stained intracellularly with appropriate antibodies in freshly fixed brain slices treated ex vivo with Brefeldin A. The cell phenotype also will be identified, TLR expression determined, and glial/neuronal apoptosis verified ..
  8. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor W Hsu; Fiscal Year: 2010
    ..Thus, as defects in intracellular transport pathways are being appreciated as the cause for a growing number of human diseases, our findings will likely be broadly applicable to understanding and treating these diseases. ..
  9. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2007
    ..Thus, as defects in intracellular transport pathways are being appreciated as the cause for a growing number of human diseases, our findings will likely be broadly applicable to understanding and treating these diseases. ..
  10. CLONOTYPIC REGULATION OF ENCEPHALITOGENIC T LYMPHOCYTES
    Halina Offner; Fiscal Year: 2001
    ..2-39-59 reactive T cells and antibodies will be inhibited by monoclonal antibodies, brefeldin A, and anti-sense DNA...
  11. RADICAL MEDIATED CYCLIZATION REACTIONS
    Ken Feldman; Fiscal Year: 1993
    ..Syntheses of the antitumor antibiotic brefeldin A and The antileukemic agent rocaglamide, which each utilize intramolecular variants of the cyclo- pentannelation ..
  12. STUDIES OF SYNAPSE FORMATION
    LYNN LANDMESSER; Fiscal Year: 2007
    ..designed to define the role of the 140 and/or 120 kD isoform of NCAM in the down regulation of the immature, brefeldin A and L-type Ca+2 channel sensitive, immature vesicle cycling/release mechanism from the axon...