brefeldin a

Summary

Summary: A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.

Top Publications

  1. ncbi The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
    Niko Geldner
    ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
    Cell 112:219-30. 2003
  2. ncbi Auxin transport inhibitors block PIN1 cycling and vesicle trafficking
    N Geldner
    , , Auf der Morgenstelle 3, , Germany
    Nature 413:425-8. 2001
  3. ncbi An ARF-GEF acting at the Golgi and in selective endocytosis in polarized plant cells
    Ooi Kock Teh
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Nature 448:493-6. 2007
  4. pmc A critical role of a cellular membrane traffic protein in poliovirus RNA replication
    George A Belov
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000216. 2008
  5. pmc bfr1+, a novel gene of Schizosaccharomyces pombe which confers brefeldin A resistance, is structurally related to the ATP-binding cassette superfamily
    K Nagao
    Department of Biotechnology, University of Tokyo, Japan
    J Bacteriol 177:1536-43. 1995
  6. pmc Brefeldin A: deciphering an enigmatic inhibitor of secretion
    Andreas Nebenführ
    Department of Botany, University of Tennessee, Knoxville, Tennessee 37996 1100, USA
    Plant Physiol 130:1102-8. 2002
  7. pmc Human papillomavirus type 16 infection of human keratinocytes requires clathrin and caveolin-1 and is brefeldin a sensitive
    Valerie Laniosz
    Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
    J Virol 83:8221-32. 2009
  8. pmc Synaptic vesicle recycling at CNS snapses without AP-2
    Sung Hyun Kim
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA
    J Neurosci 29:3865-74. 2009
  9. ncbi GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat
    Kazumasa Kawamoto
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Traffic 3:483-95. 2002
  10. pmc Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor
    George A Belov
    Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6120, Bethesda, MD 20892 8011, USA
    J Virol 79:7207-16. 2005

Detail Information

Publications286 found, 100 shown here

  1. ncbi The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
    Niko Geldner
    ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
    Cell 112:219-30. 2003
    ..The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of ..
  2. ncbi Auxin transport inhibitors block PIN1 cycling and vesicle trafficking
    N Geldner
    , , Auf der Morgenstelle 3, , Germany
    Nature 413:425-8. 2001
    ..In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.
  3. ncbi An ARF-GEF acting at the Golgi and in selective endocytosis in polarized plant cells
    Ooi Kock Teh
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Nature 448:493-6. 2007
    ..at the Golgi but is also required for selective internalization from the plasma membrane in the presence of brefeldin A (BFA)...
  4. pmc A critical role of a cellular membrane traffic protein in poliovirus RNA replication
    George A Belov
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000216. 2008
    ..Virus replication is inhibited by brefeldin A (BFA), implicating some components(s) of the cellular secretory pathway in virus growth...
  5. pmc bfr1+, a novel gene of Schizosaccharomyces pombe which confers brefeldin A resistance, is structurally related to the ATP-binding cassette superfamily
    K Nagao
    Department of Biotechnology, University of Tokyo, Japan
    J Bacteriol 177:1536-43. 1995
    ..a Schizosaccharomyces pombe gene, bfr1+, which on a multicopy plasmid vector, pDB248', confers resistance to brefeldin A (BFA), an inhibitor of intracellular protein transport...
  6. pmc Brefeldin A: deciphering an enigmatic inhibitor of secretion
    Andreas Nebenführ
    Department of Botany, University of Tennessee, Knoxville, Tennessee 37996 1100, USA
    Plant Physiol 130:1102-8. 2002
  7. pmc Human papillomavirus type 16 infection of human keratinocytes requires clathrin and caveolin-1 and is brefeldin a sensitive
    Valerie Laniosz
    Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
    J Virol 83:8221-32. 2009
    ..An understanding of the steps involved in HPV16 sorting and trafficking opens up the possibility of developing novel approaches to interfere with HPV16 infection and reduce the burden of papillomavirus diseases including cervical cancer...
  8. pmc Synaptic vesicle recycling at CNS snapses without AP-2
    Sung Hyun Kim
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA
    J Neurosci 29:3865-74. 2009
    ..These results demonstrate that in the absence of AP-2, SV proteins still become endocytosed, and synaptic vesicle recycling remains operational...
  9. ncbi GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat
    Kazumasa Kawamoto
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Traffic 3:483-95. 2002
    ..This process is blocked by brefeldin A, which is an inhibitor of guanine nucleotide exchange factors for ADP-ribosylation factor...
  10. pmc Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor
    George A Belov
    Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6120, Bethesda, MD 20892 8011, USA
    J Virol 79:7207-16. 2005
    ..Replication of poliovirus is sensitive to brefeldin A (BFA), a fungal metabolite known to prevent normal function of the ADP-ribosylation factor (ARF) family of ..
  11. ncbi Dissection of brefeldin A-sensitive and -insensitive steps in apicoplast protein targeting
    Amy DeRocher
    Seattle Biomedical Research Institute, 307 Westlake Avenue N, Suite 500 Seattle, WA 98109, USA
    J Cell Sci 118:565-74. 2005
    ..Incubation with brefeldin A, which blocks ER-to-Golgi trafficking by inhibiting a GTP exchange factor required for retrograde trafficking, ..
  12. ncbi Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism
    Elena Mossessova
    Howard Hughes Medical Institute, Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell 12:1403-11. 2003
    ..b>Brefeldin A (BFA) is a fungal metabolite that binds to the ARF1*GDP*Sec7 complex and blocks GEF activity at an early stage ..
  13. ncbi Intracellular localization and in vivo trafficking of p24A and p23
    R Blum
    Institute of Physiology II, Medical Biochemistry and Department of Anatomy, University of the Saarland, D 66421 Homburg Saar, Germany
    J Cell Sci 112:537-48. 1999
    ..This study therefore gives new insights into the localization and trafficking behavior of the two COPI-binding proteins p24A and p23...
  14. ncbi BFA-induced compartments from the Golgi apparatus and trans-Golgi network/early endosome are distinct in plant cells
    Sheung Kwan Lam
    Department of Biology and Molecular Biotechnology Program, Centre for Cell and Developmental Biology, State China Key Laboratory for Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant J 60:865-81. 2009
    b>Brefeldin A (BFA) is a useful tool for studying protein trafficking and identifying organelles in the plant secretory and endocytic pathways...
  15. ncbi Characterization of Aspergillus nidulans RabC/Rab6
    Areti Pantazopoulou
    Department of Molecular and Cellular Medicine, Centro de Investigaciones Biologicas CSIC, Ramiro de Maeztu 9, Madrid 28040, Spain
    Traffic 12:386-406. 2011
    ..rabCΔ mutants, showing markedly impaired apical extension, have conspicuously fragmented, brefeldin A-insensitive early and late GEs, indicating that the Golgi network organization requires RabC...
  16. ncbi Endoplasmic reticulum stress stimulates the expression of cyclooxygenase-2 through activation of NF-kappaB and pp38 mitogen-activated protein kinase
    Jui Hsiang Hung
    Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China
    J Biol Chem 279:46384-92. 2004
    ..Tunicamycin and brefeldin A, two ER stress inducers, increased the expression of COX-2 in ML-1 or MCF-7 cells...
  17. pmc Organization and dynamics of the Aspergillus nidulans Golgi during apical extension and mitosis
    Areti Pantazopoulou
    Departamento de Microbiologia Molecular, Centro de Investigaciones Biologicas C S I C, Madrid 28040, Spain
    Mol Biol Cell 20:4335-47. 2009
    ..b>Brefeldin A transiently collapses late and early GEs into distinct aggregates containing Sec7/mRFP-PH(OSBP) and GrhA-GFP, ..
  18. ncbi Functional diversification of closely related ARF-GEFs in protein secretion and recycling
    Sandra Richter
    ZMBP, Entwicklungsgenetik, Tubingen, Germany
    Nature 448:488-92. 2007
    ....
  19. ncbi Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors
    A Togawa
    Pulmonary Critical Care Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 274:12308-15. 1999
    ..Two major families of ARF GEPs are known: approximately 200-kDa molecules that are inhibited by brefeldin A (BFA), a fungal metabolite that blocks protein secretion and causes apparent disintegration of Golgi structure, ..
  20. ncbi Intracellular trafficking of Potato leafroll virus movement protein in transgenic Arabidopsis
    Florian Vogel
    Friedrich Alexander Universitat Erlangen Nurnberg, Staudtstrasse 5, 91058 Erlangen, Germany
    Traffic 8:1205-14. 2007
    ..Interestingly, inhibition of the 26S proteasome led to aggregation of MP17 in aggresome-like structures. Formation of these structures could be inhibited by colchicine, as was shown for aggresomes in mammalian cells...
  21. pmc The endosomal system of plants: charting new and familiar territories
    David G Robinson
    Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Plant Physiol 147:1482-92. 2008
  22. ncbi A GFP-based assay reveals a role for RHD3 in transport between the endoplasmic reticulum and Golgi apparatus
    Huanquan Zheng
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK
    Plant J 37:398-414. 2004
    ..Inhibition of secGFP transport between the ER and plasma membrane (PM), either by Brefeldin A (BFA) treatment or by genetic intervention results in increased intracellular secGFP accumulation accompanied ..
  23. ncbi BFA effects are tissue and not just plant specific
    David G Robinson
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
    Trends Plant Sci 13:405-8. 2008
    b>Brefeldin A (BFA) is one of the most popular drugs used by researchers for studies on secretion and endocytosis because it interferes with specific vesicle coat proteins via action on a guanine nucleotide exchange factor...
  24. pmc F-actin-dependent endocytosis of cell wall pectins in meristematic root cells. Insights from brefeldin A-induced compartments
    Frantisek Baluska
    Plant Cell Biology, Institute of Botany, University of Bonn, Kirschallee 1, D 53115 Bonn, Germany
    Plant Physiol 130:422-31. 2002
    b>Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries...
  25. ncbi Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46
    Lionel Breuza
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    J Biol Chem 279:47242-53. 2004
    ..we have developed a novel procedure for the purification of ERGIC membranes from HepG2 cells treated with brefeldin A, a drug known to accumulate cycling proteins in the ERGIC...
  26. pmc Epac mediates PACAP-dependent long-term depression in the hippocampus
    Jeanne Ster
    CNRS, UMR 5203, Institut de Genomique Fonctionnelle, Montpellier, France
    J Physiol 587:101-13. 2009
    ..Altogether these results provided evidence for a new form of hippocampal LTD...
  27. pmc Uptake of a fluorescent marker in plant cells is sensitive to brefeldin A and wortmannin
    Neil Emans
    Institute for Molecular Biotechnology, Biology VII, Rheinisch Westfälische Technische Hochschule Aachen, Worringerweg 1, D 52074 Aachen, Germany
    Plant Cell 14:71-86. 2002
    ..FM1-43 uptake in suspension cells was stimulated more than twofold by brefeldin A and inhibited approximately 0.4-fold by wortmannin...
  28. pmc Dynein supports motility of endoplasmic reticulum in the fungus Ustilago maydis
    Roland Wedlich-Soldner
    Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Strasse, D 35043 Marburg, Germany
    Mol Biol Cell 13:965-77. 2002
    ..Both the gradient and the Golgi apparatus were sensitive to brefeldin A or benomyl treatment, suggesting that the gradient represents microtubule-dependent vesicle trafficking between ..
  29. pmc Localization and recycling of gp27 (hp24gamma3): complex formation with other p24 family members
    J Fullekrug
    Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 10:1939-55. 1999
    ..Second, treatment with brefeldin A caused gp27 to relocate into peripheral structures positive for both KDEL receptor and COPII...
  30. pmc PINOID positively regulates auxin efflux in Arabidopsis root hair cells and tobacco cells
    Sang Ho Lee
    Department of Biology, Chungnam National University, Daejeon 305 764, Korea
    Plant Cell 18:1604-16. 2006
    ..Localization of PID or PIN3 at the cell boundary was disrupted by brefeldin A and staurosporine...
  31. pmc Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination
    O Staub
    Hospital for Sick Children, Division of Respiratory Research, Toronto, Ontario, Canada
    EMBO J 16:6325-36. 1997
    ..retention or recycling of ENaC and incorporation of new channels at the plasma membrane, as determined by Brefeldin A treatment...
  32. ncbi Munc18 interacting proteins: ADP-ribosylation factor-dependent coat proteins that regulate the traffic of beta-Alzheimer's precursor protein
    Karen Hill
    Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322 3050, USA
    J Biol Chem 278:36032-40. 2003
    ..We propose that MINTs are a family of Arf-dependent, vesicle-coat proteins that can regulate the traffic of beta-APP...
  33. pmc Caveolar endocytosis of simian virus 40 is followed by brefeldin A-sensitive transport to the endoplasmic reticulum, where the virus disassembles
    Leonard C Norkin
    Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003 5720, USA
    J Virol 76:5156-66. 2002
    ..Additionally, transport of SV40 to the ER, as well as infection, is sensitive to brefeldin A. This drug acts by specifically inhibiting the ARF1 GTPase, which is known to regulate assembly of COPI coat ..
  34. ncbi Brefeldin A, but not monensin, completely blocks CD69 expression on mouse lymphocytes: efficacy of inhibitors of protein secretion in protocols for intracellular cytokine staining by flow cytometry
    S Nylander
    Cell Biology and Biochemistry, Preclinical R and D, ASTRA Hässle, Molndal, Sweden
    J Immunol Methods 224:69-76. 1999
    ..The two most widely used reagents are monensin and brefeldin A (BFA). These reagents differ somewhat in their mode of action, which might explain their different effects...
  35. pmc Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking
    M Steegmaier
    Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305 5345, USA
    Mol Biol Cell 10:1957-72. 1999
    ..Immunoprecipitation of VAMP4 from rat brain detergent extracts revealed that VAMP4 exists in a complex containing syntaxin 6. Converging lines of evidence implicate a role for VAMP4 in TGN-to-endosome transport...
  36. pmc The brefeldin A resistance protein Bfr1p is a component of polyribosome-associated mRNP complexes in yeast
    B D Lang
    Graduate Program in Biochemistry, Emory University School of Medicine, 1462 Clifton Road NE, Atlanta, GA 30322, USA
    Nucleic Acids Res 29:2567-74. 2001
    The yeast gene BFR1 was originally isolated from a genetic screen for high-copy suppressors of brefeldin A-induced lethality in Saccharomyces cerevisiae...
  37. pmc Arf3 is activated uniquely at the trans-Golgi network by brefeldin A-inhibited guanine nucleotide exchange factors
    Florin Manolea
    Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
    Mol Biol Cell 21:1836-49. 2010
    ..These results suggest that Arf3 plays a unique function at the TGN that likely involves recruitment by a specific receptor...
  38. ncbi Ganglioside GD3 traffics from the trans-Golgi network to plasma membrane by a Rab11-independent and brefeldin A-insensitive exocytic pathway
    Pilar Maria Crespo
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, 5000 Cordoba, Argentina
    J Biol Chem 279:47610-8. 2004
    ..did not partition into GEM domains in the Golgi complex and trafficked from TGN to plasma membrane by a brefeldin A-insensitive exocytic pathway...
  39. ncbi Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistance
    T G Turi
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510
    J Biol Chem 269:24229-36. 1994
    The fungal metabolite brefeldin A (BFA) causes the inhibition of protein secretion and the disruption of the structure and function of the Golgi complex in mammalian cells...
  40. pmc TNO1 is involved in salt tolerance and vacuolar trafficking in Arabidopsis
    Sang Jin Kim
    Department of Genetics, Iowa State University, Ames, Iowa 50011, USA
    Plant Physiol 156:514-26. 2011
    ..The tno1 mutant had delayed formation of the brefeldin A (BFA) compartment in cotyledons upon application of BFA, suggesting less efficient membrane fusion processes in ..
  41. ncbi Differential effects of the brefeldin A analogue (6R)-hydroxy-BFA in tobacco and Arabidopsis
    Markus Langhans
    Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, D 69120 Heidelberg, Germany
    J Exp Bot 62:2949-57. 2011
    The effects of two brefeldin A (BFA) analogues (BFA lactam; 6(R)-hydroxy-BFA) on plant cells were tested...
  42. ncbi Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells
    Joachim Seemann
    Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
    Science 295:848-51. 2002
    ..We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein...
  43. ncbi Proteomics on brefeldin A-treated Arabidopsis roots reveals profilin 2 as a new protein involved in the cross-talk between vesicular trafficking and the actin cytoskeleton
    Tomáš Takáč
    Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Department of Cell Biology, Palacký University, Olomouc, Czech Republic
    J Proteome Res 10:488-501. 2011
    ..Here, proteomic and cell biology approaches were applied to study effects of brefeldin A (BFA), an inhibitor of vesicle recycling and secretion, in Arabidopsis roots...
  44. pmc Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective alpha(1b)-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers
    Meritxell Canals
    Molecular Pharmacology Group, Neuroscience and Molecular Pharmacology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G128QQ, Scotland, UK
    Biochem J 417:161-72. 2009
    ....
  45. ncbi Involvement of caspase-2 and caspase-9 in endoplasmic reticulum stress-induced apoptosis: a role for the IAPs
    Herman H Cheung
    Apoptosis Research Centre, Children s Hospital of Eastern Ontario Research Institute II, Ottawa, Ontario, Canada K1H 8L1
    Exp Cell Res 312:2347-57. 2006
    ..Here we show that brefeldin A and tunicamycin-mediated ER stress lead to caspase-dependent apoptosis involving caspase-2...
  46. ncbi The antibody to GD3 ganglioside, R24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment. Inhibitory effect of brefeldin A and monensin
    Ramiro Iglesias-Bartolomé
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC UNC CONICET, Departamento de Quimica Biologica, Universidad Nacional de Cordoba, Argentina
    FEBS J 273:1744-58. 2006
    ..exits the recycling compartment en route to the plasma membrane by a dynamin 2-dependent pathway sensitive to brefeldin A and monensin...
  47. ncbi Transmembrane domain length determines intracellular membrane compartment localization of syntaxins 3, 4, and 5
    R T Watson
    Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA
    Am J Physiol Cell Physiol 281:C215-23. 2001
    ..These data support a model wherein short transmembrane domains (< or =17 amino acids) direct the cis-Golgi localization of syntaxins, whereas long transmembrane domains (> or =23 amino acids) direct plasma membrane localization...
  48. ncbi ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1
    S E Langille
    Program in Molecular Medicine, Worcester, Massachusetts 01605, USA
    J Biol Chem 274:27099-104. 1999
    ..GTP loading of ARF6 in both control cells and in response to GRP1 or cytohesin-1 was insensitive to brefeldin A, consistent with previous data on endogenous ARF6 exchange activity...
  49. ncbi The SPCA1 Ca2+ pump and intracellular membrane trafficking
    Massimo Micaroni
    Laboratory of Intracellular Traffic, Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Via Nazionale 8 A, 66030 Santa Maria Imbaro Chieti, Italy
    Traffic 11:1315-33. 2010
    ..and delays retrograde redistribution of the GA glycosylation enzymes into the endoplasmic reticulum caused by brefeldin A; however, exit of these enzymes from the endoplasmic reticulum is not affected...
  50. pmc PDMP blocks brefeldin A-induced retrograde membrane transport from golgi to ER: evidence for involvement of calcium homeostasis and dissociation from sphingolipid metabolism
    J W Kok
    Department of Physiological Chemistry, University of Groningen, Groningen Institute for Drug Studies GIDS, 9713 AV Groningen, The Netherlands
    J Cell Biol 142:25-38. 1998
    ..of sphingolipid biosynthesis, D,L-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol (PDMP), inhibits brefeldin A (BFA)-induced retrograde membrane transport from Golgi to endoplasmic reticulum (ER)...
  51. ncbi The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast
    A Peyroche
    Service de Biochimie et Génétique Moléculaire, Bat 142, Departement de Biologie Cellulaire et Moleculaire, CEA Saclay, 91191 Gif sur Yvette, France
    J Cell Sci 114:2241-53. 2001
    ..In addition, the structure of Golgi elements was severely perturbed in gea mutants. We conclude that Gea1p and Gea2p play an important role in the structure and functioning of the Golgi apparatus in yeast...
  52. pmc Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell
    T Fujimoto
    Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    J Cell Biol 152:1079-85. 2001
    ..b>Brefeldin A treatment induced further accumulation of caveolin-2 along with caveolin-1 in LD...
  53. pmc Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11
    B Sönnichsen
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 149:901-14. 2000
    ..We propose that endosomes are organized as a mosaic of different Rab domains created through the recruitment of specific effector proteins, which cooperatively act to generate a restricted environment on the membrane...
  54. pmc Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae
    Elena V Gazina
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    J Virol 76:11113-22. 2002
    ..b>Brefeldin A (BFA) has been shown to strongly inhibit RNA replication of poliovirus but not of encephalomyocarditis virus (..
  55. pmc Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activation
    Monique H Verheije
    Virology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    PLoS Pathog 4:e1000088. 2008
    ..We now show that MHV replication is sensitive to brefeldin A (BFA)...
  56. pmc Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex
    A R Skop
    Laboratory of Molecular Biology, University of Colorado, Boulder, CO 80309, USA
    Curr Biol 11:735-46. 2001
    ..In this study, we investigated the possibility that targeted secretion in the vicinity of the spindle midzone is required for the execution of the terminal phase of cytokinesis...
  57. ncbi Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A
    Elena Ortiz-Zapater
    Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad de Valencia, Avda Vicente Andres Estelles s n, 46100 Burjassot Valencia, Spain
    Plant J 48:757-70. 2006
    ..In contrast, treatment with brefeldin A, which inhibits recycling from endosomes back to the plasma membrane in plant cells, leads to the accumulation ..
  58. ncbi Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation
    Chisa Shinotsuka
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biol Chem 277:9468-73. 2002
    ..A fungal metabolite, brefeldin A (BFA), inhibits ARF-GEFs and leads to redistribution of coat proteins from membranes to the cytoplasm and ..
  59. pmc Characterization of class I and II ADP-ribosylation factors (Arfs) in live cells: GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1
    Justin Chun
    Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada
    Mol Biol Cell 19:3488-500. 2008
    ..Live cell imaging detected Arfs on peripheral puncta that also contained Golgi-specific brefeldin A (BFA) resistance factor (GBF) 1 and the ERGIC marker p58...
  60. pmc Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain
    N Morinaga
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:12856-60. 1996
    b>Brefeldin A (BFA) inhibited the exchange of ADP ribosylation factor (ARF)-bound GDP for GTP by a Golgi-associated guanine nucleotide-exchange protein (GEP) [Helms, J.B. & Rothman, J.E...
  61. pmc Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells
    Yu Chung Tse
    Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Plant Cell 16:672-93. 2004
    ..b>Brefeldin A induced the YFP-labeled Golgi stacks but not the YFP-marked PVCs to form typical enlarged structures...
  62. pmc Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets
    A G Ostermeyer
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Cell Biol 152:1071-8. 2001
    ..Third, incubation of cells with brefeldin A caused caveolin-1 to accumulate in the droplets...
  63. pmc Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi
    Ting Kuang Niu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:1213-22. 2005
    ..The drug brefeldin A (BFA), an uncompetitive inhibitor of the exchange reaction that binds to an Arf-GDP-Arf GEF complex, stabilizes ..
  64. pmc ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis
    Mi Hee Lee
    Center for Plant Intracellular Trafficking, Pohang University of Science and Technology, Pohang, 790 784, Korea
    Plant Physiol 129:1507-20. 2002
    ..The treatment of protoplasts with brefeldin A had exactly the same effect as Arf1[T31N] on various intracellular trafficking pathways...
  65. ncbi Different effects of aluminum on the actin cytoskeleton and brefeldin A-sensitive vesicle recycling in root apex cells of two maize varieties differing in root elongation rate and aluminum tolerance
    Montse Amenós
    Institut für Zelluläre und Molekulare Botanik, Universitat Bonn, Kirschallee 1, D 53115 Bonn, Germany
    Plant Cell Physiol 50:528-40. 2009
    ..Visualization of boron-cross-linked rhamnogalacturonan II (RGII)-containing brefeldin A (BFA) compartments revealed that Al inhibited the formation of these compartments, especially in variety 16x36...
  66. ncbi Regulation of Arf activation: the Sec7 family of guanine nucleotide exchange factors
    James E Casanova
    Department of Cell Biology, University of Virginia Health System, Charlottesville, VA 22908 0732, USA
    Traffic 8:1476-85. 2007
    ..Here we review recent progress in our understanding of the structure, localization and biology of the different classes of Arf GEFs...
  67. ncbi The Intra- and intercellular movement of Melon necrotic spot virus (MNSV) depends on an active secretory pathway
    Ainhoa Genoves
    Instituto Biologia Molecular y Celular de Plantas, Universidad Politecnica, Universidad Politecnica de Valencia CSIC, Avenida de los Naranjos s n, 46022 Valencia, Spain
    Mol Plant Microbe Interact 23:263-72. 2010
    ..Hence, the secretory pathway inhibitor, Brefeldin A (BFA), and the overexpression of the GTPase-defective mutant of Sar1p, Sar1[H74L], fully retained the p7B ..
  68. ncbi Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens
    Fang yi Cheng
    Department of Horticultural Science, North Carolina State University, Raleigh, NC 27695 7609, USA
    Planta 230:1093-103. 2009
    ..After SA treatment, MTD was not detected in the Golgi apparatus, and its SA-induced secretion was resistant to brefeldin A, an inhibitor of Golgi-mediated protein transport...
  69. ncbi Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: involvement of specific residues of the Sec7 domain
    A Peyroche
    Service de Biochimie et Génétique Moléculaire, CEA Saclay, Gif sur Yvette, France
    Mol Cell 3:275-85. 1999
    We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and ..
  70. pmc Exo1: a new chemical inhibitor of the exocytic pathway
    Yan Feng
    Institute of Chemistry and Cell Biology, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6469-74. 2003
    ..Like Brefeldin A (BFA), Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less ..
  71. ncbi Effects of brefeldin A on the localization of Tobamovirus movement protein and cell-to-cell movement of the virus
    Yuko Tagami
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro, Tokyo 153 8902, Japan
    Virology 361:133-40. 2007
    ..Treatment with brefeldin A (BFA), which disrupts ER-to-Golgi transport, inhibited the formation of irregularly shaped and filamentous ..
  72. pmc ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes
    C E Ooi
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    J Cell Biol 142:391-402. 1998
    ..Association of AP-3 with membranes in vitro was enhanced by GTPgammaS and inhibited by brefeldin A (BFA), an inhibitor of ARF1 guanine nucleotide exchange...
  73. ncbi Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuole
    Ombretta Foresti
    Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, via Bassini 15, 20133 Milano, Italy, EU
    Mol Plant 1:1067-76. 2008
    ..is delivered to the vacuole and fragmented, in a process that is inhibited by the secretory traffic inhibitor brefeldin A. Moreover, a fusion between GFP and a domain of the maize storage protein gamma-zein involved in zein ..
  74. pmc p200 ARF-GEP1: a Golgi-localized guanine nucleotide exchange protein whose Sec7 domain is targeted by the drug brefeldin A
    S J Mansour
    Department of Cell Biology, University of Alberta, MSB 5 14, Edmonton, AB T6G 2H7, Canada
    Proc Natl Acad Sci U S A 96:7968-73. 1999
    The drug brefeldin A (BFA) disrupts protein traffic and Golgi morphology by blocking activation of ADP ribosylation factors (ARFs) through an unknown mechanism...
  75. ncbi Brefeldin A action and recovery in Chlamydomonas are rapid and involve fusion and fission of Golgi cisternae
    E Hummel
    Department of Cell Biology, Heidelberg Institute for Plant Sciences HIP, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
    Plant Biol (Stuttg) 9:489-501. 2007
    ..These structures then divide by vertical fission to produce Golgi stacks of normal size and morphology roughly 60 min after drug wash-out...
  76. pmc Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway
    Claude Saint-Jore-Dupas
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6037, IFRMP 23, GDR 2590, UFR des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant Cell 18:3182-200. 2006
    ..We conclude that the localization of N-glycan processing enzymes corresponds to an assembly line in the early secretory pathway and depends on both TMD length and signals in the cytoplasmic tail...
  77. ncbi Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport
    John F Presley
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 417:187-93. 2002
    ..This role for Arf1/coatomer might provide a model for investigating the behaviour of other coat protein systems within cells...
  78. ncbi Brefeldin A-induced neurotoxicity in cultured spinal cord neurons
    Seiji Kikuchi
    Department of Neurology, Hokkaido University School of Medicine, Sapporo, Hokkaido, Japan
    J Neurosci Res 71:591-9. 2003
    b>Brefeldin A (BFA) is a fungus metabolite that is known to cause the disassembly of the Golgi complex and apoptosis in exposed cells, both of which have been suggested as playing roles in the pathogenesis of neurodegenerative diseases, ..
  79. pmc Interstitial adenosine triphosphate modulates muscle afferent nerve-mediated pressor reflex
    Jianhua Li
    Penn State Heart and Vascular Institute, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Muscle Nerve 38:972-7. 2008
    ..05 vs. control). In contrast, glibenclamide (an inhibitor of the ATP-binding cassette protein), monensin, and brefeldin A, which interfere with vesicular formation (or trafficking) and inhibit exocytosis, did not significantly affect ..
  80. ncbi Regulation of brefeldin A-induced ER stress and apoptosis by mitochondrial NADP⁺-dependent isocitrate dehydrogenase
    Jung Lee Moon
    School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu 702 701, Republic of Korea
    Biochem Biophys Res Commun 417:760-4. 2012
    b>Brefeldin A (BFA), an endoplasmic reticulum (ER)-Golgi transport inhibitor, has been shown to cause accumulation of proteins in the ER, ER stress, and ultimately apoptosis...
  81. ncbi On the action of Brefeldin A on Sec7-stimulated membrane-recruitment and GDP/GTP exchange of Arf proteins
    J Cherfils
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Biochem Soc Trans 33:635-8. 2005
    ..The drug BFA (Brefeldin A) was used to trap the complex that initiates the exchange reaction, providing a structural basis for its ..
  82. pmc Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas
    A Oprins
    Department of Cell Biology, Medical School, Utrecht University, The Netherlands
    J Cell Biol 121:49-59. 1993
    ..After 10 min of Brefeldin A treatment 91% of beta-COP was cytoplasmic and only 7% associated with membranes of the Golgi complex...
  83. ncbi Brefeldin a inhibits circadian remodeling of chloroplast structure in the dinoflagellate gonyaulax
    Nasha Nassoury
    Institut de recherche en biologie végétale, Departement de sciences biologiques, Universite de Montreal, 4101 Sherbrooke est, Montreal, Quebec, Canada H1X2B2
    Traffic 6:548-61. 2005
    ..Rubisco transit through the Golgi in dinoflagellates; hence, we blocked protein import into the plastids using Brefeldin A (BFA) to explore the mechanism for plastid remodeling...
  84. pmc GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 149:81-94. 2000
    ..Treatment with brefeldin A or overexpression of dominant-negative ADP ribosylation factor 1 (ARF1) caused dissociation of GGAs from ..
  85. pmc Quantitative ER <--> Golgi transport kinetics and protein separation upon Golgi exit revealed by vesicular integral membrane protein 36 dynamics in live cells
    T Dahm
    Light Microscopy Group, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Biol Cell 12:1481-98. 2001
    ....
  86. ncbi Structural integrity of the Golgi stack is essential for normal secretory functions of rat parotid acinar cells: effects of brefeldin A and okadaic acid
    Hideaki Tamaki
    Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
    J Histochem Cytochem 50:1611-23. 2002
    We examined the effects of specific inhibitors, brefeldin A (BFA) and okadaic acid (OA), on the ultrastructural organization of the Golgi apparatus and distributions of amylase, Golgi-associated proteins, and cathepsin D in the rat ..
  87. ncbi Brefeldin A and filipin distinguish two globotriaosyl ceramide/verotoxin-1 intracellular trafficking pathways involved in Vero cell cytotoxicity
    Aye Aye Khine
    Department of Laboratory Medicine and Pathobiology, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada
    Glycobiology 14:701-12. 2004
    ..b>Brefeldin A (BFA), which prevents Golgi-dependent retrograde traffic, protects cells from low VT1 concentrations but not ..
  88. ncbi Inhibition of Japanese encephalitis virus maturation and transport in PS cells to cell surface by brefeldin A
    V J Lad
    National Institute of Virology, Indian Council of Medical Research, 20 A, Dr Ambedkar Road, P B No 11, Pune 411 001, India
    Acta Virol 46:187-90. 2002
    A brefeldin A (BFA) treatment of porcine stable kidney (PS) cells resulted in inhibition of Japanese encephalitis virus (JEV) maturation and its transport to the cell surface...
  89. pmc Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis
    Rodolfo D Vicetti Miguel
    Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224, USA
    J Immunol Methods 384:191-5. 2012
    ..trachomatis genital tract infection were used to compare the ability of brefeldin A (BFA) and monensin (MN) to concurrently trap interferon-γ (IFN-γ), tumor necrosis factor (TNF), interleukin (..
  90. ncbi Multidrug resistance phenotype conferred by overexpressing bfr2+/pad1+/sks1+ or pap1+ genes and mediated by bfr1+ gene product, a structural and functional homologue of P-glycoprotein in Schizosaccharomyces pombe
    M Arioka
    Department of Biotechnology, University of Tokyo, Japan
    Biosci Biotechnol Biochem 62:390-2. 1998
    ..These findings suggest that bfr1+ acts as the most downstream effector of the multidrug resistance conferred by bfr2+ and pap1+ genes...
  91. pmc Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera
    Christophe Ritzenthaler
    Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
    Plant Cell 14:237-61. 2002
    b>Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus...
  92. pmc Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor
    E Meacci
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Proc Natl Acad Sci U S A 94:1745-8. 1997
    ..Cytohesin-1 also increased ARF1 binding to a Golgi fraction, but its effect was not inhibited by brefeldin A (BFA), a drug that reversibly inhibits Golgi function...
  93. ncbi Brefeldin A inhibits vesicular MHC class I processing in resting but not in CpG- and disruption-activated DC
    Liying Chen
    Microbiology, Tumor and Cell Biology MTC, Karolinska Institute, 171 77 Stockholm, Sweden
    Mol Immunol 46:158-65. 2008
    ..Here we investigate the sensitivity of this VP to Brefeldin A (BFA), a fungal metabolite which inhibits vesicular trafficking by preventing the activation of some ADP-..
  94. ncbi Plasmodium vivax and Plasmodium chabaudi: intraerythrocytic traffic of antigenically homologous proteins involves a brefeldin A-sensitive secretory pathway
    C Bracho
    Instituto Venezolano de Investigaciones Cientificas IVIC, Caracas, Venezula
    Eur J Cell Biol 80:164-70. 2001
    ..In the presence of brefeldin A (BFA), secretion of antigenically homologous Pc-190 into the host cell cytoplasm was inhibited and the antigen ..
  95. ncbi Brefeldin A influences the cell surface abundance and intracellular pools of low and high ouabain affinity Na+, K(+)-ATPase alpha subunit isoforms in articular chondrocytes
    A Mobasheri
    Department of Biomedical Sciences, School of Biosciences, University of Westminster, London, United Kingdom
    Histol Histopathol 14:427-38. 1999
    ..05) upregulation of Na+, K(+)-ATPase density and treatment with various pharmacological inhibitors (Brefeldin A, monensin and cycloheximide) significantly (p < 0.001) blocked the upregulation...
  96. pmc Assembly and polarized release of Punta Toro virus and effects of brefeldin A
    S Y Chen
    Department of Microbiology, University of Alabama, Birmingham 35294
    J Virol 65:1427-39. 1991
    ..We have examined PTV glycoprotein transport, assembly, and release and the effects of brefeldin A (BFA) on these processes...
  97. pmc Low-level doxorubicin resistance in P-glycoprotein-negative human pancreatic tumour PSN1/ADR cells implicates a brefeldin A-sensitive mechanism of drug extrusion
    V N Verovski
    Cancer Research Unit, Department of Radiotherapy, Academic Hospital, Free University Brussels, Belgium
    Br J Cancer 73:596-602. 1996
    ..In addition, these defects were reversible by the vesicular traffic inhibitors brefeldin A, fluoroaluminate and nocodazole...
  98. ncbi Brefeldin A disrupts asymmetric spindle positioning in mouse oocytes
    Ling Wang
    Ottawa Health Research Institute, Ottawa Hospital, Civic Campus, 1053 Carling Avenue, Ottawa, Canada
    Dev Biol 313:155-66. 2008
    ..We show here that treating denuded mouse oocytes with brefeldin A, an inhibitor of Golgi-based membrane fusion, abolished the asymmetric positioning of the metaphase I spindle ..
  99. ncbi The role of de novo ceramide synthesis in the mechanism of action of the tricyclic xanthate D609
    Ryan J Perry
    Department of Pediatrics, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia, Canada
    J Lipid Res 45:164-73. 2004
    ..In D609-treated CHO-K1 cells, sphingomyelin synthesis was stimulated by brefeldin A, and C5-DMB-ceramide transport to the Golgi apparatus was blocked, indicating ceramide accumulation in the ..
  100. ncbi Brefeldin A enhances Helicobacter pylori vacuolating cytotoxin-induced vacuolation of epithelial cells
    Richard H Argent
    Institute of Infection, Immunity and Inflammation, and the Wolfson Digestive Diseases Centre, University of Nottingham, Queen s Medical Centre, Nottingham, NG7 2UH, UK
    FEMS Microbiol Lett 237:163-70. 2004
    ..We show that the Golgi-disturbing agent brefeldin A (BFA) enhances VacA-induced vacuolation of epithelial cells by Helicobacter pylori co-culture and, importantly, ..
  101. ncbi Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1
    Melanie L Styers
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, GA 30322, USA
    J Cell Sci 119:3643-54. 2006
    ..We find that treatment of cells with Brefeldin A, an inhibitor of specific stages of membrane transport, causes changes in the organization of vimentin ..

Research Grants62

  1. ER stress: role in myocyte apoptosis and myocardial remodeling
    Krishna Singh; Fiscal Year: 2013
    ..Well-known pharmacological ER stressors (brefeldin A, thapsigargin and tunicamycin) activated GSK-32 and increased cardiac myocyte apoptosis...
  2. Mechanism of Apoptosis and Inhibition of Tumor Progression and Metastasis by Par-
    Vivek M Rangnekar; Fiscal Year: 2012
    ..The apoptotic activity was diminished in the CM from Par-4 transfectants treated with brefeldin A, indicating Par-4 and SAC secretion (or sPar-4 and sSAC, respectively) occurs by the classical pathway (i.e...
  3. Enteropathogenic E. coli effector proteins in intestinal tight junction recovery
    LILA GOLLOGLY; Fiscal Year: 2013
    ..IEC will be transfected to express NleA, or treated with brefeldin A, and the effects on TJ recovery following calcium chelation will be measured...
  4. A Small Molecule Approach Toward Understanding Bacterial Toxin Transport
    JOSE BERNARDO SAENZ; Fiscal Year: 2010
    ..One novel compound, B06, produces a brefeldin A-like dispersal of the Golgi, and preliminary evidence suggests that it could serve as a useful tool in ..
  5. Pathogenesis of Lyme Borreliosis in the Rhesus Monkey
    MARIO PHILIPP; Fiscal Year: 2007
    ..be stimulated ex vivo with lipoprotein or live spirochetes in the presence of the protein-secretion blocker Brefeldin A. After fixation and cutting of frozen sections, the cytokine/mediator-producing cell phenotype will be ..
  6. FUNGAL POLYKETIDE SYNTHASES
    CHARLES SIH; Fiscal Year: 2001
    ..of the fungal PKSs involved in the biosynthesis of the biologically active compounds mevinolin (lovastatin) and brefeldin A. We will strive to establish how these two multifunctional proteins, which are likely to contain sets of ..
  7. ABNORMAL TERMINAL COLON OF THE 1S/1S MUTANT MOUSE
    TAUBE ROTHMAN; Fiscal Year: 2000
    ..They will use immunocytochemistry to identify these cells. They also will use a drug called brefeldin A which blocks secretion of proteins and should enhance the identification of the cells...
  8. ARF PROTEINS IN CELLULAR REGULATION OF PHOSPHOLIPASE D
    Guillermo Romero; Fiscal Year: 2000
    ..Cells that overexpress mutated receptors do not show this behavior. Likewise, the isoprenoid Brefeldin A, an agent that disrupts intracellular membrane traffic and whose effects are linked to the inhibition of ARF ..
  9. PROCESSING OF PROINSULIN/INSULIN BY B-CELL ORGANELLES
    Philippe Halban; Fiscal Year: 1993
    ..b>Brefeldin A inhibits RER-Golgi transfer but allows the reverse movement of molecules (regurgitation from the Golgi)...
  10. Engineering Biosynthesis of Brefeldin A and Its Analogs
    Weiwen Zhang; Fiscal Year: 2002
    ..b>Brefeldin A, a macrolide polyketide produced by Eupenicilium brefeldianum, exhibits a wide range of antibiotic, antifungal ..
  11. ANTIVIRAL/BIOACTIVE COMPOUNDS--SYNTHESIS AND CHEMISTRY
    ELIAS COREY; Fiscal Year: 1999
    ..agent, pseudopterosin E; (7) synthesis of the inhibitor of protein translocation and processing, brefeldin A; (8) synthesis of the inhibitors of biosynthesis of initiation factor 4D; (9) synthesis of new serotonin uptake ..
  12. CELLULAR BIOPHYSICS
    Eric Siggia; Fiscal Year: 2000
    ..The kinetics of the adsorption of the Golgi by the ER induced by Brefeldin A treatment are not diffusive and a model involving surface tension driven flow is being pursued.
  13. STUDIES OF SYNAPSE FORMATION
    LYNN LANDMESSER; Fiscal Year: 2007
    ..designed to define the role of the 140 and/or 120 kD isoform of NCAM in the down regulation of the immature, brefeldin A and L-type Ca+2 channel sensitive, immature vesicle cycling/release mechanism from the axon...
  14. Pertussis Toxin Trafficking and Processing in Cells
    NICHOLAS CARBONETTI; Fiscal Year: 2005
    ..Therefore the specific aims of this proposal are to investigate the trafficking, processing and transcytosis of PT in mammalian cells and the key features of this toxin that mediate these events. ..
  15. Role of Pertussis Toxin in Bordetella pertussis infection
    Nicholas H Carbonetti; Fiscal Year: 2010
    ..pertussis infection will increase our understanding of the pathogenic mechanisms of this organism, and may lead to novel therapeutic approaches to combat pertussis infection and disease. ..
  16. Synthesis of Macrolides, Steroids, Cyclopentanoids, etc.
    BARRY TROST; Fiscal Year: 2009
    ..These new synthetic methods apply to many structural types beyond those illustrated and constitutes a significant to gain access to complex molecular targets more easily. ..
  17. Effect of Pertussis Toxin on Cough in Guinea Pig
    NICHOLAS CARBONETTI; Fiscal Year: 2005
    ..abstract_text> ..
  18. LACTOSE SYNTHESIS REGULATION: ROLE OF GLUCOSE TRANSPORT
    PETER HANEY; Fiscal Year: 2002
    ..These will be related to changes in glucose transport activity an lactose synthesis that are essential for milk production. ..
  19. Engineering a CFTR Deficient Porcine Model of CF
    SCOTT O GRADY; Fiscal Year: 2007
    ..abstract_text> ..
  20. Genetics of SV40 Entry and Minichromosome Transport
    LEONARD NORKIN; Fiscal Year: 2008
    ..Recent studies report evidence that SV40 infection may lead to neoplastic disease in humans, including mesotheliornas, osteosarcomas, childhood brain tumors, and non-Hodgkin's Iymphoma. ..
  21. Dysfuntional antigen presenting cell responses to influenza in geriatric individu
    DAVID CANADAY; Fiscal Year: 2008
    ..Ag processing and presentation efficiency of B cells is important for them to receive optimal costimulation from CD4+ T helper cells. [unreadable] [unreadable] [unreadable]..
  22. Estrogen and Aging effects on Transvaginal Transport
    George Gorodeski; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  23. RHESUS MACAQUE MODEL OF TUBERCULOSIS
    DAVID LEWINSOHN; Fiscal Year: 2001
    ..g., CT scans, quantitative culture of mycobacteria from BAL fluids and liver biopsies) which correlate with survival. ..
  24. CD8+T cell responses to Chlamydia pneumoniae
    Benjamin Wizel; Fiscal Year: 2006
    ..pneumoniae and will suggest methods of immunological intervention in infections caused by C. pneumoniae and other chlamydial pathogens. ..
  25. Mechanisms of MHC-II antigen processing of HIV in macrophages and dendritic cells
    DAVID CANADAY; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  26. IRON BINDING PROTEINS AND CONTROL OF IRON METABOLISM
    Philip Aisen; Fiscal Year: 2001
    ..Understanding the fundamental chemistry of iron transport, storage and utilization will provide the rational basis for managing the multitude of diseases where aberrations of iron metabolism contribute to cellular injury and death. ..
  27. Connexin acetylation: Impact on gap junction function and HDAC inhibitor respons
    JEANNETTE CHLOE BULINSKI; Fiscal Year: 2010
    ..In a broader view, we will also demonstrate novel roles by which protein acetylation regulates cellular functions not directly involved with gene expression. ..
  28. Functional Characterization of Nuclear Myosin I in Transcription
    Primal de Lanerolle; Fiscal Year: 2010
    ..The experiments described in this proposal investigate the role of a new protein in transcription. They have the potential to increase our understanding of the transcription process at the molecular level ..
  29. CFTR-Ion Conduction and Gating Mechanisms
    David C Dawson; Fiscal Year: 2010
    ....
  30. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James E Casanova; Fiscal Year: 2010
    ....
  31. A new approach to xenotransplantation in primate
    Juan Contreras; Fiscal Year: 2005
    ..The research proposed in this grant will provide significant information to the xenotransplant research and the potential use of porcine islets as an alternative to human tissue for pancreatic islet transplantation. ..
  32. Intercellular signaling during terminal differentiation
    William Loomis; Fiscal Year: 2009
    ..Together these studies will add to the foundation for improvements in the diagnosis and treatment of neurological diseases. ..
  33. Analysis of apoptotic signaling induced by the mammalian endoplasmic reticulum
    JAY BREWSTER; Fiscal Year: 2008
    ..of ER stress and its signaling mechanisms will offer vital insight into the cell biology of stressed cells, and will be valuable to our understanding of diseases that result from abnormal ER function [unreadable] [unreadable] [unreadable]..
  34. Mitogen activated protein kinases regulation-PTH/PTHrP
    Hesham Tawfeek; Fiscal Year: 2008
    ..Primary osteoblastic cell culture from wild type and genetically engineered mice as well as several other biochemical and pharmacological tools will be employed to achieve the goals of the study. ..
  35. Hormonal Therapy to Increase Islet Recovery/Engraftment From Multiorgan Donors
    Juan Contreras; Fiscal Year: 2008
    ..abstract_text> ..
  36. Effects of brain-death on islet recovery and functional*
    John Thompson; Fiscal Year: 2006
    ..Overall, these studies will bring novel information about human islet biology and will provide c potential therapeutic strategy to enhance the therapeutic efficacy of PIT as a cure for diabetes. ..
  37. Antigen Processing in HIV-infected Individuals
    DAVID CANADAY; Fiscal Year: 2006
    ..e. HIV-induced deficits in both APC and T cells were potentially present), our studies will provide the first well-controlled comparison of APC function in HIV+ and HIV- persons. ..
  38. Strategy for islet recovery, functionality & engraftment
    Juan Contreras; Fiscal Year: 2006
    ..The proposed studies are expected to yield novel information about the effects of BD on isolated islet biology and to develop potential targets for therapeutic intervention, thereby enhancing the efficacy of PIT as a cure for diabetes. ..
  39. Cysteine String Protein and Acute Pancreatitis
    GUY GROBLEWSKI; Fiscal Year: 2004
    ..These studies will allow us to directly evaluate the role of CSP in the secretory pathway of acini under physiological and pathophysiological conditions. ..
  40. ANTIBODY PROBES OF CNS MITOCHONDRIAL APOPTOTIC FUNCTION
    Ronald Jemmerson; Fiscal Year: 2004
    ..The feasibility of the proposed research is supported by preliminary results and by the participation of on-site collaborators with expertise in the field of neuroscience, in particular the physiology of CNS mitochondria. ..
  41. New Perspectives in Transporter Biology-FASEB conference
    Robert Edwards; Fiscal Year: 2003
    ..Each oral session will be followed by a panel discussion of the topic. To promote the advancement of junior faculty, the most exciting poster submissions will be selected for short oral presentations during the regular oral sessions. ..
  42. CD8+ T CELLS AND MYCOPLASMA TUBERCULOSIS
    DAVID CANADAY; Fiscal Year: 2003
    ..Aim 3. To characterize the functional CD8+ T cell responses to specific proteins and peptides from in patients with active M. tuberculosis infection. ..
  43. NUCLEAR MYOSIN I
    Primal de Lanerolle; Fiscal Year: 2003
    ..This proposal will also develop a number of tools (eg: plasmids, monoclonal and anti-peptide antibodies, proteins expressed in baculovirus, knock-out cells) that will be instrumental in realizing our long range goal. ..
  44. MOLECULAR NETWORKS THAT COORDINATE DIFFERENTIATION
    William Loomis; Fiscal Year: 2003
    ..Together these studies will help to establish a signal transduction pathway that can serve as a model for studies in other systems. ..
  45. IRON TRANSPORT IN MAMMALIAN CELLS
    Philip Aisen; Fiscal Year: 2003
    ..Project 5: Structure, function and dynamics in transferrin investigates the molecular mechanisms through which the uptake and release of iron and other metals by transferrin are modulated by physiological factors. ..
  46. ASSEMBLY AND TARGETING OF EPITHELIAL P-TYPE ATPASES
    Curtis Okamoto; Fiscal Year: 2001
    ....
  47. MYOSIN LIGHT CHAIN KINASE IN SMOOTH MUSCLE PROLIFERATION
    Primal de Lanerolle; Fiscal Year: 2002
    ..This, in turn could result in improved therapy for intimal hyperplasia. ..
  48. NOVEL USE OF ZEBRAFISH FOR APOPTOSIS ASSAY
    Chuenlei Parng; Fiscal Year: 2004
    ..Complementary visual and flow cytometry based assays will also be developed to provide comprehensive analysis of apoptosis in zebrafish. ..
  49. GTPase Regulation of Smooth Muscle Contraction
    Primal de Lanerolle; Fiscal Year: 2004
    ..These experiments represent a powerful test of our hypothesis and they will provide important insights into the molecular mechanisms that regulate the calcium sensitivity of smooth muscle contraction. ..
  50. TRANSPORT OF NEUROTRANSMITTERS INTO SYNAPTIC VESICLES
    Robert Edwards; Fiscal Year: 2005
    ..Third, we will manipulate the expression, localization and regulation of the transporters in vivo to assess the role of these mechanisms in synaptic transmission and behavior. ..
  51. Nitric oxide in bladder neural-epithelial signaling
    Lori Birder; Fiscal Year: 2005
    ..Elucidation of mechanisms impacting on urothelial function may provide insights into the pathology of bladder dysfunction. ..
  52. Role of the GTPase ARF6 in Epithelial Cell Migration
    James Casanova; Fiscal Year: 2005
    ..abstract_text> ..
  53. TRANSEPITHELIAL TRANSPORT OF IMMUNOGLOBULINS
    James Casanova; Fiscal Year: 2002
    ..These studies will provide insight into the mechanisms by which macromolecules are transported across epithelia, a process that is particularly important in the mucosal immunity. ..
  54. Proteomic Biomarkers of Health Behaviors
    Paul Lampe; Fiscal Year: 2005
    ..abstract_text> ..
  55. Zebrafish Screen for Neuroprotective Anti-oxidants
    Chuenlei Parng; Fiscal Year: 2005
    ..Assessment of drug effects in a convenient vertebrate model, prior to evaluation in a complex system, such as mouse, can potentially streamline the drug development process and dramatically reduce costs. ..
  56. Functional Genomics of Dictyostelium Development
    William Loomis; Fiscal Year: 2005
    ..Predictions derived from such networks will be tested when microarray analyses are extended to include further developmental mutants. ..
  57. Cardiotoxicity Drug Screening Using Zebrafish
    Chuenlei Parng; Fiscal Year: 2004
    ..Zebrafish drug screens can serve as an intermediate step between cell-based and mammalian testing, streamlining the drug development timeline. ..
  58. ASSAY FOR DETERMINING DRUG TOXICITY
    Chuenlei Parng; Fiscal Year: 2004
    ..abstract_text> ..
  59. REGULATION OF EXTRACELLULAR SUPEROXIDE DISMUTASE BY PROT
    Russell Bowler; Fiscal Year: 2004
    ..This will not only define new aspects of the pathogenesis and regulation of vascular tone and inflammation, but can open new approaches to pharmacologically control these events. ..
  60. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..