squalene

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Top Publications

  1. ncbi Development and evaluation of AS03, an Adjuvant System containing α-tocopherol and squalene in an oil-in-water emulsion
    Nathalie Garçon
    GlaxoSmithKline Biologicals, Rixensart, Belgium
    Expert Rev Vaccines 11:349-66. 2012
  2. ncbi Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase
    Saloni Gill
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia
    Cell Metab 13:260-73. 2011
  3. pmc Influence of squalene on lipid particle/droplet and membrane organization in the yeast Saccharomyces cerevisiae
    Miroslava Spanova
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    Biochim Biophys Acta 1821:647-53. 2012
  4. ncbi Safety of MF59-adjuvanted A/H1N1 influenza vaccine in pregnancy: a comparative cohort study
    Terho Heikkinen
    Department of Pediatrics, University of Turku, Turku, Finland
    Am J Obstet Gynecol 207:177.e1-8. 2012
  5. pmc Effect of lipid particle biogenesis on the subcellular distribution of squalene in the yeast Saccharomyces cerevisiae
    Miroslava Spanova
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 II, A 8010 Graz, Austria
    J Biol Chem 285:6127-33. 2010
  6. ncbi From squalene to brassinolide: the steroid metabolic and signaling pathways across the plant kingdom
    Cécile Vriet
    CropDesign NV, a BASF Plant Science Company, 9052 Gent, Belgium
    Mol Plant 6:1738-57. 2013
  7. ncbi Fatty acid profile, tocopherol, squalene and phytosterol content of walnuts, almonds, peanuts, hazelnuts and the macadamia nut
    L S Maguire
    Department of Food and Nutritional Sciences, University College Cork, Ireland
    Int J Food Sci Nutr 55:171-8. 2004
  8. ncbi Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats
    K H Sabeena Farvin
    Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri P O, Cochin 682 029, India
    Pharmacol Res 50:231-6. 2004
  9. ncbi A new nanomedicine of gemcitabine displays enhanced anticancer activity in sensitive and resistant leukemia types
    L Harivardhan Reddy
    Universite Paris Sud XI, Faculte de Pharmacie, UMR CNRS 8612, IFR 141, 92296 Chatenay Malabry Cedex, France
    J Control Release 124:20-7. 2007
  10. ncbi The antioxidant network of the stratum corneum
    J J Thiele
    Department of Dermatology and Allergology, Friedrich Schiller University, Jena, Germany
    Curr Probl Dermatol 29:26-42. 2001

Detail Information

Publications272 found, 100 shown here

  1. ncbi Development and evaluation of AS03, an Adjuvant System containing α-tocopherol and squalene in an oil-in-water emulsion
    Nathalie Garçon
    GlaxoSmithKline Biologicals, Rixensart, Belgium
    Expert Rev Vaccines 11:349-66. 2012
    AS03 is an Adjuvant System composed of α-tocopherol, squalene and polysorbate 80 in an oil-in-water emulsion...
  2. ncbi Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase
    Saloni Gill
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia
    Cell Metab 13:260-73. 2011
    ..b>Squalene monooxygenase (SM) catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before ..
  3. pmc Influence of squalene on lipid particle/droplet and membrane organization in the yeast Saccharomyces cerevisiae
    Miroslava Spanova
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    Biochim Biophys Acta 1821:647-53. 2012
    In a previous study (Spanova et al., 2010, J. Biol. Chem., 285, 6127-6133) we demonstrated that squalene, an intermediate of sterol biosynthesis, accumulates in yeast strains bearing a deletion of the HEM1 gene...
  4. ncbi Safety of MF59-adjuvanted A/H1N1 influenza vaccine in pregnancy: a comparative cohort study
    Terho Heikkinen
    Department of Pediatrics, University of Turku, Turku, Finland
    Am J Obstet Gynecol 207:177.e1-8. 2012
    ..The 2009-2010 A/H1N1 pandemic provided a unique setting to study the safety of MF59-adjuvanted vaccination in pregnancy...
  5. pmc Effect of lipid particle biogenesis on the subcellular distribution of squalene in the yeast Saccharomyces cerevisiae
    Miroslava Spanova
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 II, A 8010 Graz, Austria
    J Biol Chem 285:6127-33. 2010
    b>Squalene belongs to the group of isoprenoids and is a precursor for the synthesis of sterols, steroids, and ubiquinones...
  6. ncbi From squalene to brassinolide: the steroid metabolic and signaling pathways across the plant kingdom
    Cécile Vriet
    CropDesign NV, a BASF Plant Science Company, 9052 Gent, Belgium
    Mol Plant 6:1738-57. 2013
    ..We also discuss emerging insights into the regulations of these pathways, their interactions with other hormonal pathways and multiple environmental signals, and the putative nature of sterols as signaling molecules. ..
  7. ncbi Fatty acid profile, tocopherol, squalene and phytosterol content of walnuts, almonds, peanuts, hazelnuts and the macadamia nut
    L S Maguire
    Department of Food and Nutritional Sciences, University College Cork, Ireland
    Int J Food Sci Nutr 55:171-8. 2004
    ..Nuts also contain other potentially cardioprotective constituents including phytosterols, tocopherols and squalene. In the present study, the total oil content, peroxide value, composition of fatty acids, tocopherols, ..
  8. ncbi Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats
    K H Sabeena Farvin
    Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri P O, Cochin 682 029, India
    Pharmacol Res 50:231-6. 2004
    This study was designed to examine the effects of squalene on tissue antioxidant status in isoproterenol-induced myocardial infarction in male albino rats...
  9. ncbi A new nanomedicine of gemcitabine displays enhanced anticancer activity in sensitive and resistant leukemia types
    L Harivardhan Reddy
    Universite Paris Sud XI, Faculte de Pharmacie, UMR CNRS 8612, IFR 141, 92296 Chatenay Malabry Cedex, France
    J Control Release 124:20-7. 2007
    ..The preclinical data obtained in vitro and in vivo with SQdFdC NA demonstrate that this nanomedicine represents a new therapeutic system for the effective treatment of leukemia...
  10. ncbi The antioxidant network of the stratum corneum
    J J Thiele
    Department of Dermatology and Allergology, Friedrich Schiller University, Jena, Germany
    Curr Probl Dermatol 29:26-42. 2001
    ....
  11. pmc Immunogenicity and safety of the influenza A/H1N1 2009 inactivated split-virus vaccine in young and older adults: MF59-adjuvanted vaccine versus nonadjuvanted vaccine
    Hee Jin Cheong
    Division of Infectious Diseases, Department of Internal Medicine, Korea University Guro Hospital, Korea University College of Medicine, 97 Gurodong Gil, Guro Gu, Seoul 152 703, South Korea
    Clin Vaccine Immunol 18:1358-64. 2011
    ..75-μg, MF59 adjuvanted vaccine priming schedule. Following a two-dose priming schedule, the increase in hemagglutinin inhibition titers was higher with MF59 adjuvanted vaccine than with nonadjuvanted vaccine...
  12. ncbi Assessment of the immunogenicity and safety of varying doses of an MF59®-adjuvanted cell culture-derived A/H1N1 pandemic influenza vaccine in Japanese paediatric, adult and elderly subjects
    Hiroyuki Fukase
    CPC Clinic, Medipolis Medical Research Institute, Kagoshima, Japan
    Vaccine 30:5030-7. 2012
    ....
  13. ncbi The adjuvants aluminum hydroxide and MF59 induce monocyte and granulocyte chemoattractants and enhance monocyte differentiation toward dendritic cells
    Anja Seubert
    Novartis Vaccines, Siena, Italy
    J Immunol 180:5402-12. 2008
    ....
  14. pmc Effects of overproduction of the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase on squalene synthesis in Saccharomyces cerevisiae
    K A Donald
    Department of Cellular Physiology, Babraham Institute, Cambridge, United Kingdom
    Appl Environ Microbiol 63:3341-4. 1997
    ..This overproduction leads to an enhancement of squalene production, implying that HMG-R has been deregulated...
  15. ncbi Squalene emulsions for parenteral vaccine and drug delivery
    Christopher B Fox
    Infectious Disease Research Institute, Seattle, WA 98104, USA
    Molecules 14:3286-312. 2009
    b>Squalene is a linear triterpene that is extensively utilized as a principal component of parenteral emulsions for drug and vaccine delivery. In this review, the chemical structure and sources of squalene are presented...
  16. pmc Squalene-hopene cyclases
    Gabriele Siedenburg
    Institute for Microbiology, University of Stuttgart, Stuttgart, Germany
    Appl Environ Microbiol 77:3905-15. 2011
    ..They are biochemically synthesized from linear precursors (squalene, 2,3-oxidosqualene) in only one enzymatic step that is catalyzed by squalene-hopene cyclase (SHC) or ..
  17. ncbi Cytoprotective effects of DL-alpha-lipoic acid or squalene on cyclophosphamide-induced oxidative injury: an experimental study on rat myocardium, testicles and urinary bladder
    Tarek M K Motawi
    Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
    Food Chem Toxicol 48:2326-36. 2010
    The present study aimed to evaluate the role of DL-alpha-lipoic acid (LA) and squalene (SQ) on oxidative cardiac, testicular and urotoxic damage induced by cyclophosphamide (CP)...
  18. pmc Photo-oxidation products of skin surface squalene mediate metabolic and inflammatory responses to solar UV in human keratinocytes
    Vladimir Kostyuk
    Laboratory of Tissue Engineering and Skin Pathophysiology, Istituto Dermopatico dell Immacolata IDI IRCCS, Rome, Italy
    PLoS ONE 7:e44472. 2012
    ..The decay of photo-sensitive lipid-soluble components, alpha-tocopherol, squalene (Sq), and cholesterol in SSL was analysed and products of squalene photo-oxidation (SqPx) were quantitatively ..
  19. ncbi Squalene: A natural triterpene for use in disease management and therapy
    L Harivardhan Reddy
    Univ Paris Sud XI, Faculte de Pharmacie, UMR CNRS 8612, 92296 Chatenay Malabry Cedex, France
    Adv Drug Deliv Rev 61:1412-26. 2009
    b>Squalene is a natural lipid belonging to the terpenoid family and a precursor of cholesterol biosynthesis. It is synthesized in humans and also in a wide array of organisms and substances, from sharks to olives and even bran, among others...
  20. ncbi Cardioprotective effect of squalene on lipid profile in isoprenaline-induced myocardial infarction in rats
    K H Sabeena Farvin
    Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri PO, Cochin, India
    J Med Food 9:531-6. 2006
    We studied the cardioprotective effect of squalene on isoprenaline-induced myocardial infarction in male albino rats with respect to changes in the levels of lipid components in plasma and heart tissue...
  21. ncbi Preclinical toxicology (subacute and acute) and efficacy of a new squalenoyl gemcitabine anticancer nanomedicine
    L Harivardhan Reddy
    Universite Paris Sud XI, Faculte de Pharmacie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 8612, Institut Fédératif de Recherche 141, 92296 Chatenay Malabry Cedex, France
    J Pharmacol Exp Ther 325:484-90. 2008
    ..These data support the candidature of SQgem for clinical trials...
  22. pmc Characterization of antibodies induced by vaccination with hepatitis C virus envelope glycoproteins
    Ranjit Ray
    Department of Internal Medicine and Vaccine and Treatment Evaluation Unit, Saint Louis University, St Louis, Missouri, USA
    J Infect Dis 202:862-6. 2010
    ..Neutralizing serum samples had increased affinity levels and displayed >2-fold higher specific activity levels to well-characterized epitopes on E1/E2, especially to the hypervariable region 1 of E2...
  23. ncbi Vaccine adjuvants alum and MF59 induce rapid recruitment of neutrophils and monocytes that participate in antigen transport to draining lymph nodes
    Samuele Calabro
    Novartis Vaccines and Diagnostics, Siena, Italy
    Vaccine 29:1812-23. 2011
    ..Further studies will reveal whether other single cell types are crucial or whether the different recruited cell populations are redundant with overlapping functions...
  24. ncbi Enhancement of gemcitabine affinity for biomembranes by conjugation with squalene: differential scanning calorimetry and Langmuir-Blodgett studies using biomembrane models
    Francesco Castelli
    Dipartimento di Scienze Chimiche, Universita degli Studi di Catania, Viale Andrea Doria 6, 95125 Catania, Italy
    J Colloid Interface Sci 316:43-52. 2007
    Molecular interactions between gemcitabine, alone or conjugated with squalene to form the gem-squalene prodrug, with dimyristoylphosphatidylcholine have been investigated by differential scanning calorimetry and Langmuir film balance ..
  25. pmc MF59-adjuvanted H5N1 vaccine induces immunologic memory and heterotypic antibody responses in non-elderly and elderly adults
    Angelika Banzhoff
    Novartis Vaccines, Marburg, Germany
    PLoS ONE 4:e4384. 2009
    ..Pathogenic avian influenza virus (H5N1) has the potential to cause a major global pandemic in humans. Safe and effective vaccines that induce immunologic memory and broad heterotypic response are needed...
  26. ncbi MF59-adjuvanted versus non-adjuvanted influenza vaccines: integrated analysis from a large safety database
    Michele Pellegrini
    Global Clinical Research and Development, Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Vaccine 27:6959-65. 2009
    ..Adding adjuvants such as MF59((R)) to influenza vaccines can enhance the immune response. This analysis evaluated the safety profile of MF59-adjuvanted [(+)MF59] compared with non-adjuvanted [(-)MF59] vaccines in a large clinical database...
  27. pmc Safety and immunogenicity of HCV E1E2 vaccine adjuvanted with MF59 administered to healthy adults
    Sharon E Frey
    Saint Louis University School of Medicine, Division of Infectious Diseases and Immunology, St Louis, MO 63104, USA
    Vaccine 28:6367-73. 2010
    ..We describe the first in humans clinical trial of this prophylactic HCV vaccine...
  28. ncbi The validity of serum squalene and non-cholesterol sterols as surrogate markers of cholesterol synthesis and absorption in type 2 diabetes
    Piia Simonen
    Department of Medicine, Division of Internal Medicine, University of Helsinki, Helsinki, Finland
    Atherosclerosis 197:883-8. 2008
    ..In type 2 diabetes (T2D) cholesterol metabolism is perturbed, and the role of squalene and non-cholesterol sterols has not been related to absolute cholesterol metabolism in detail.
  29. ncbi Visceral obesity is associated with high levels of serum squalene
    Paula Peltola
    Department of Medicine, University of Kuopio, Kuopio, Finland
    Obesity (Silver Spring) 14:1155-63. 2006
    ..To investigate the impact of visceral obesity on cholesterol metabolism in normoglycemic offspring of patients with type 2 diabetes...
  30. ncbi A new MF59-adjuvanted influenza vaccine enhances the immune response in the elderly with chronic diseases: results from an immunogenicity meta-analysis
    Angelika Banzhoff
    Clinical Research and Medical Affairs, Chiron Vaccines, Marburg, Germany
    Gerontology 49:177-84. 2003
    ..The elderly are at a higher risk of morbidity and mortality associated with influenza infection than younger adults, but get less protection from conventional vaccination...
  31. ncbi Cross-protection by MF59-adjuvanted influenza vaccine: neutralizing and haemagglutination-inhibiting antibody activity against A(H3N2) drifted influenza viruses
    Filippo Ansaldi
    CIRI IV, Department of Health Sciences, University of Genoa, Genoa, Italy
    Vaccine 26:1525-9. 2008
    ..Thus, MF59-adjuvanted vaccine confers greater immunogenicity than non-adjuvanted vaccines in vulnerable populations...
  32. pmc Cloning, solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1
    Sungwon Lee
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112, USA
    J Bacteriol 190:3808-16. 2008
    b>Squalene synthase (SQS) is a bifunctional enzyme that catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to give presqualene diphosphate (PSPP) and the subsequent rearrangement of PSPP to squalene...
  33. ncbi Predictors of immune response and reactogenicity to AS03B-adjuvanted split virion and non-adjuvanted whole virion H1N1 (2009) pandemic influenza vaccines
    Nick J Andrews
    Department of Statistics, Modelling and Economics, Health Protection Services, Health Protection Agency, Colindale, London NW9 5EQ, UK
    Vaccine 29:7913-9. 2011
    ..Reduction in the interval between doses for earlier protection would be at the cost of reduced immunogenicity. The effect of seasonal vaccine on immunogenicity merits further investigation...
  34. ncbi MF59 is a safe and potent vaccine adjuvant that enhances protection against influenza virus infection
    Derek T O'Hagan
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Expert Rev Vaccines 6:699-710. 2007
    ..MF59 has been shown to be more potent for both antibody and T-cell responses than aluminum-based adjuvants. MF59 has broad potential to be used as a safe and effective vaccine adjuvant for a wide range of vaccine types...
  35. ncbi Adjuvanted seasonal influenza vaccines and perpetual viral metamorphosis: the importance of cross-protection
    Filippo Ansaldi
    Department of Health Sciences, University of Genoa CIRI IV, Inter University Research Centre on Influenza, Via Pastore 1, 16132 Genoa, Italy
    Vaccine 27:3345-8. 2009
    ..Promising results were obtained using a new generation of oil-in-water emulsion adjuvants, named AS, offering cross-protection against heterologous challenge in ferrets...
  36. ncbi Safety of MF59-adjuvanted versus non-adjuvanted influenza vaccines in children and adolescents: an integrated analysis
    Steven Black
    Center for Global Health and Division of Infectious Diseases, Cincinnati Children s Hospital, 3333 Burnet Ave, Cincinnati, OH 45326, USA
    Vaccine 28:7331-6. 2010
    ..These data support the safety of MF59-adjuvanted influenza vaccines in the pediatric population...
  37. ncbi Peroxidated squalene induces the production of inflammatory mediators in HaCaT keratinocytes: a possible role in acne vulgaris
    Monica Ottaviani
    San Gallicano Dermatological Institute IRCCS, Rome, Italy
    J Invest Dermatol 126:2430-7. 2006
    ..can be responsible for the induction of inflammatory processes, HaCaT keratinocytes were treated with peroxidated squalene. NF-kappaB activation, secretion, and expression of IL-6, as well as peroxisome proliferator-activated receptor ..
  38. ncbi Impact of corticosteroids on the immune response to a MF59-adjuvanted influenza vaccine in elderly COPD-patients
    A de Roux
    Department of Chest and Infectious Diseases, Heckeshorn, Helios Kliniken Emil von Behring, Berlin, Germany
    Vaccine 24:1537-42. 2006
    ..Systemic steroids did not influence the antibody response towards the MF59-adjuvanted influenza vaccine. We found that the strains included in the vaccine showed varying long-term immunogenicity...
  39. ncbi Phytosterol, squalene, tocopherol content and fatty acid profile of selected seeds, grains, and legumes
    E Ryan
    Department of Food and Nutritional Sciences, University College, Cork, Ireland
    Plant Foods Hum Nutr 62:85-91. 2007
    ..lipid fraction of plant-based foods is a potential source of bioactive components such as phytosterols, squalene, and tocopherols...
  40. ncbi Conversion of squalene to the pentacarbocyclic hopene
    Dirk J Reinert
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstr 21, D 79104 Freiburg, Germany
    Chem Biol 11:121-6. 2004
    The membrane protein squalene-hopene cyclase was cocrystallized with 2-azasqualene and analyzed by X-ray diffraction to 2.13 A resolution...
  41. ncbi Laser desorption/ionization time-of-flight mass spectrometry of squalene in oil samples
    Carlo G Zambonin
    Rapid Commun Mass Spectrom 20:325-7. 2006
  42. ncbi Attenuation of cyclophosphamide induced toxicity by squalene in experimental rats
    Subramanian Senthilkumar
    Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India
    Chem Biol Interact 160:252-60. 2006
    ..In this study, the protective role of squalene (SQ) towards the tissue defense system in the toxicity induced by CP (150 mg/kg b.w...
  43. ncbi Application of supercritical fluid chromatography in the quantitative analysis of minor components (carotenes, vitamin E, sterols, and squalene) from palm oil
    Yuen May Choo
    Malaysian Palm Oil Board MPOB, No 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia
    Lipids 40:429-32. 2005
    ..with a UV variable-wavelength detector to isolate the minor components (carotenes, vitamin E, sterols, and squalene) in crude palm oil (CPO) and the residual oil from palm-pressed fiber is reported...
  44. ncbi Airway gene transfer using cationic emulsion as a mucosal gene carrier
    Tae Woo Kim
    Graduate School of Medicine, Korea University, 1, 5 Ka, Anam Dong, Sungbuk Ku, Seoul 136 791, Korea
    J Gene Med 7:749-58. 2005
    ..In this study, we use a cationic emulsion system, which is physically stable and facilitates the transfer of genes in the presence of up to 90% serum, as a mucosal gene carrier...
  45. ncbi Squalene does not exhibit a chemopreventive activity and increases plasma cholesterol in a Wistar rat hepatocarcinogenesis model
    Clarissa Scolastici
    Department of Pathology, Faculty of Medicine, Sao Paulo State University, Botucatu, Brazil
    Nutr Cancer 50:101-9. 2004
    The eventual chemopreventive effect of squalene (SQ), a triterpene present in olive oil, was evaluated when administered to Wistar rats during a period comprising the initiation and selection/promotion of the "resistant hepatocyte&..
  46. ncbi Accumulation of squalene is associated with the clustering of lipid droplets
    Minh T Ta
    School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia
    FEBS J 279:4231-44. 2012
    The isoprenoid squalene is an important precursor for the biosynthesis of sterols. The cellular storage of squalene and its impact on membrane properties have been the subject of recent investigations...
  47. ncbi Molecular characterization of Glycine max squalene synthase genes in seed phytosterol biosynthesis
    Hanh T M Nguyen
    Division of Plant Sciences and National Center for Soybean Biotechnology, University of Missouri, Columbia, MO 65211, USA
    Plant Physiol Biochem 73:23-32. 2013
    The reaction catalyzed by squalene synthase (EC.2.5.1...
  48. ncbi The monotopic membrane protein human oxidosqualene cyclase is active as monomer
    Armin Ruf
    F Hoffmann La Roche Ltd, Pharma Research Discovery, CH 4070 Basel, Switzerland
    Biochem Biophys Res Commun 315:247-54. 2004
    ..The availability of this active recombinant human membrane protein is a very important step on the way to a more detailed functional and structural characterization of OSCs...
  49. ncbi Balancing kinetic and thermodynamic control: the mechanism of carbocation cyclization by squalene cyclase
    Ramkumar Rajamani
    Department of Chemistry and Supercomputing Institute, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 125:12768-81. 2003
    ..a combined quantum mechanical and molecular mechanical (QM/MM) potential have been carried out to investigate the squalene-to-hopene carbocation cyclization mechanism in squalene-hopene cyclase (SHC)...
  50. ncbi Formation of the C ring in the lanosterol biosynthesis from squalene
    B Andes Hess
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA
    Org Lett 5:165-7. 2003
    ..to elucidate the mechanism of the formation of the five-membered C ring in the biosynthesis of lanosterol from squalene. A conformational analysis of the side chain containing the double bond indicated that the conformer that should ..
  51. pmc Assessment of squalene adjuvanted and non-adjuvanted vaccines against pandemic H1N1 influenza in children 6 months to 17 years of age
    Timo Vesikari
    Vaccine Research Center, University of Tampere, Tampere, Finland
    Hum Vaccin Immunother 8:1283-92. 2012
    ..We tested H1N1 vaccines with or without AF03, a squalene-based adjuvant, in children.   Two randomized, open-label, trials were conducted...
  52. ncbi Screening and characterization of squalene-producing thraustochytrids from Hong Kong mangroves
    Qian Li
    Department of Biology and Kwong Living Trust Food Safety and Analysis Laboratory, Hong Kong Baptist University, Kowloon Tong, Hong Kong
    J Agric Food Chem 57:4267-72. 2009
    Eighteen strains of thraustochytrids were newly isolated from Hong Kong mangroves, and their fatty acid and squalene contents were analyzed. All strains could grow well heterotrophically with glucose as the sole carbon source...
  53. ncbi An adjuvanted pandemic influenza H1N1 vaccine provides early and long term protection in health care workers
    Abdullah S Madhun
    Influenza Centre, The Gade Institute, University of Bergen, N 5021 Bergen, Norway
    Vaccine 29:266-73. 2010
    ..A rapid induction of protective antibody is important in reducing community spread of pandemic influenza and in helping maintain the integrity of the health care system during the pandemic...
  54. pmc Presence of wax esters and squalene in human saliva
    Andrew J Brasser
    Dows Institute for Dental Research, University of Iowa, Iowa City, IA 52242, USA
    Arch Oral Biol 56:588-91. 2011
    ..The purpose of this study was to determine the presence and relative composition of neutral lipids in human saliva...
  55. ncbi 5-Nitrofuranes and 5-nitrothiophenes with anti-Trypanosoma cruzi activity and ability to accumulate squalene
    Alejandra Gerpe
    Departamento de Quimica Organica, Facultad de Ciencias Facultad de Química, Universidad de la Republica, Igua 4225, 11400 Montevideo, Uruguay
    Bioorg Med Chem 17:7500-9. 2009
    ..cruzi through oxidative stress and inhibition of parasite ergosterol biosynthesis, specifically at the level of squalene epoxidase...
  56. ncbi MF59 adjuvant: the best insurance against influenza strain diversity
    Derek T O'Hagan
    Novartis Vaccines and Diagnostics, Cambridge, MA, USA
    Expert Rev Vaccines 10:447-62. 2011
    ....
  57. ncbi Confirmation of the presence of squalene in human eyelid lipid by heteronuclear single quantum correlation spectroscopy
    Douglas Borchman
    Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, 301 E Muhammad Ali Blvd, Louisville, KY, 40202, USA
    Lipids 48:1269-77. 2013
    1)H-NMR spectroscopy has been used to quantify squalene in meibum and sebum...
  58. ncbi Heterogeneous oxidation of squalene film by ozone under various indoor conditions
    L Petrick
    Technion, ITT Civil and Environmental Engineering, Technion City, Haifa, Israel
    Indoor Air 19:381-91. 2009
    ..conditions (ozone concentration, temperature, relative humidity (RH), and the presence of NO(x)) on heterogeneous squalene oxidation were studied with Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy...
  59. ncbi Increased squalene concentrations in the clitoral gland during the estrous cycle in rats: an estrus-indicating scent mark?
    Shanmugam Achiraman
    Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, Tamilnadu, India
    Theriogenology 76:1676-83. 2011
    b>Squalene in the rat clitoral gland is reported to be semi-volatile and may serve as a chemo-signal. The objective was to determine squalene concentrations in the clitoral gland throughout the reproductive cycle...
  60. ncbi Pharmacologic inhibition of squalene synthase and other downstream enzymes of the cholesterol synthesis pathway: a new therapeutic approach to treatment of hypercholesterolemia
    Stephanie Seiki
    Department of Internal Medicine, University of Colorado Health Science Center, Denver, USA
    Cardiol Rev 17:70-6. 2009
    ..b>Squalene synthase is another enzyme that is downstream to HMG-CoA reductase in the cholesterol synthesis pathway and ..
  61. ncbi Influence of squalene feeding on plasma leptin, testosterone & blood pressure in rats
    Yuxi Liu
    College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China
    Indian J Med Res 129:150-3. 2009
    Obesity, hyperlipidaemia and hypercholesterolaemia are known risk factors in the pathogenesis of hypertension. Squalene has been shown to reduce serum cholesterol and triglycerides in dogs although its therapeutic use in high BP and obese ..
  62. ncbi Proteomics and gene expression analyses of squalene-supplemented mice identify microsomal thioredoxin domain-containing protein 5 changes associated with hepatic steatosis
    Adela Ramírez-Torres
    Departamento Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto de Investigación Sanitaria de Aragón IIS, Universidad de Zaragoza, Spain
    J Proteomics 77:27-39. 2012
    b>Squalene is an abundant hydrocarbon present in virgin olive oil. Previous studies showed that its administration decreased atherosclerosis and steatosis in male apoE-knock-out mice...
  63. ncbi Biological importance and applications of squalene and squalane
    Se Kwon Kim
    Department of Chemistry, Pukyong National University, Busan, Republic of Korea
    Adv Food Nutr Res 65:223-33. 2012
    b>Squalene is a polyunsaturated hydrocarbon with a formula of C₃₀H₅₀. Squalene can be found in certain fish oils, especially shark liver oil, in high amounts and some vegetable oils in relatively smaller amounts...
  64. ncbi Squalene in a sex-dependent manner modulates atherosclerotic lesion which correlates with hepatic fat content in apoE-knockout male mice
    Natalia Guillen
    Departamento Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Spain
    Atherosclerosis 197:72-83. 2008
    b>Squalene is an intermediate of cholesterol biosynthesis which can be obtained from the diet where it is abundant, for example, in olive oil...
  65. ncbi Fast and easy in vitro screening assay for cholesterol biosynthesis inhibitors in the post-squalene pathway
    Martin Giera
    Department Pharmazie, Zentrum für Pharmaforschung, Ludwig Maximilians Universitat, Munchen, Butenandtstrasse 5 13, 81377 Munich, Germany
    Steroids 72:633-42. 2007
    A whole-cell assay for screening cholesterol biosynthesis inhibitors in the post-squalene pathway has been developed. HL 60 cells were incubated for 24h with test substances...
  66. ncbi Transmembrane diffusion of gemcitabine by a nanoparticulate squalenoyl prodrug: an original drug delivery pathway
    L Bildstein
    Univ Paris Sud, UMR CNRS 8612, IFR 141 ITFM, Faculte de Pharmacie, Chatenay Malabry, F 92296, France
    J Control Release 147:163-70. 2010
    We have designed an amphiphilic prodrug of gemcitabine (dFdC) by its covalent coupling to a derivative of squalene, a natural lipid...
  67. ncbi Squalene and squalane emulsions as adjuvants
    A C Allison
    SurroMed Corporation, 1060 East Meadow Circle, Palo Alto, California 94303, USA
    Methods 19:87-93. 1999
    Microfluidized squalene or squalane emulsions are efficient adjuvants, eliciting both humoral and cellular immune responses. Microfluidization stabilizes the emulsions and allows sterilization by terminal filtration...
  68. ncbi Squalane is in the midplane of the lipid bilayer: implications for its function as a proton permeability barrier
    Thomas Hauss
    Hahn Meitner Institute, Glienicker Strasse 100, D 14109, Berlin, Germany
    Biochim Biophys Acta 1556:149-54. 2002
    ..The alkaliphiles are unique in that they contain up to 40 mol% isoprenes in their membranes including 10-11 mol% squalene [J. Bacteriol. 168 (1986) 334]. Squalene is a polyisoprene hydrocarbon without polar groups...
  69. pmc Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis
    N Shibata
    Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 98:2244-9. 2001
    b>Squalene epoxidase, a membrane-associated enzyme that converts squalene to squalene 2,3-oxide, plays an important role in the maintenance of cholesterol homeostasis...
  70. ncbi Squalene content and antioxidant activity of Terminalia catappa leaves and seeds
    Ting Fu Ko
    Graduate Institute of Food Science, National Chiayi University, Chiayi, Taiwan, Republic of China
    J Agric Food Chem 50:5343-8. 2002
    b>Squalene was identified by gas chromatography-mass spectrometry and high-performance liquid chromatography (HPLC) spiking analyses in the supercritical CO(2) extracts of freeze-dried abscisic leaves of Terminalia catappa L...
  71. ncbi Inhibition of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis by dietary olive oil and squalene
    T J Smith
    Laboratory for Cancer Research, College of Pharmacy, Rutgers University, Piscataway, NJ 08854 8020, USA
    Carcinogenesis 19:703-6. 1998
    ..b>Squalene, a characteristic compound in olive oil, is an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase ..
  72. ncbi Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B
    Shigeru Okada
    Laboratory of Marine Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Arch Biochem Biophys 422:110-8. 2004
    ..Botryococcenes are structurally similar to squalene raising the possibility of a common enzyme for the biosynthesis of both. While B...
  73. ncbi Impact of prior or concomitant seasonal influenza vaccination on MF59-adjuvanted H1N1v vaccine (Focetria) in adult and elderly subjects
    R Gasparini
    Department of Health Sciences, Genoa University, Genoa, Italy
    Int J Clin Pract 64:432-8. 2010
    ..We investigated prior seasonal vaccination in adult and elderly subjects, and concomitant vaccination with seasonal vaccine in adults, on the tolerability and immunogenicity of the Novartis MF59-adjuvanted H1N1v vaccine, Focetria...
  74. ncbi Antibodies to squalene in US Navy Persian Gulf War veterans with chronic multisymptom illness
    Christopher J Phillips
    Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, CA 92106 3521, USA
    Vaccine 27:3921-6. 2009
    ..One of the many possible exposures suspected of causing chronic multisymptom illnesses Gulf War veterans is squalene, thought to be present in anthrax vaccine...
  75. ncbi Cloning, heterologous expression, and enzymological characterization of human squalene monooxygenase
    B P Laden
    Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536 0082, USA
    Arch Biochem Biophys 374:381-8. 2000
    The cDNA for human squalene monooxygenase, a key enzyme in the committed pathway for cholesterol biosynthesis, was amplified from a human liver cDNA library and cloned, and the protein was expressed in Escherichia coli and purified...
  76. ncbi An alternative renewable source of squalene for use in emulsion adjuvants
    Luis A Brito
    Novartis Vaccines and Diagnostics, 350 Massachusetts Ave, Cambridge, MA 02139, USA
    Vaccine 29:6262-8. 2011
    ..to reduce reactogenicity of emulsion adjuvants lead to the development of oil in water emulsions based on squalene. MF59 is an oil-in-water emulsion that is a component of an approved influenza product in Europe...
  77. pmc Production of a polyunsaturated isoprenoid wax ester during aerobic metabolism of squalene by Marinobacter squalenivorans sp. nov
    Jean François Rontani
    Laboratoire d Océanographie et de Biogéochimie UMR 6535, Centre d Océanologie de Marseille OSU, Campus de Luminy, Case 901, 13288 Marseille, France
    Appl Environ Microbiol 69:4167-76. 2003
    ..during the aerobic degradation of squalene by a Marinobacter strain, 2Asq64, isolated from the marine environment...
  78. ncbi Recombinant squalene synthase. A mechanism for the rearrangement of presqualene diphosphate to squalene
    Brian S J Blagg
    Department of Chemistry, University of Utah, 315 South 1400 East Room 2020, Salt Lake City, Utah 84112, USA
    J Am Chem Soc 124:8846-53. 2002
    b>Squalene synthase (SQase) catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to form presqualene diphosphate (PSPP) and the subsequent rearrangement and NADPH-dependent reduction of PSPP to squalene (SQ)...
  79. ncbi Supercritical carbon dioxide extraction of oil and squalene from amaranthus grain
    Han Ping He
    Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, People s Republic of China
    J Agric Food Chem 51:7921-5. 2003
    Supercritical carbon dioxide (SC CO(2)) was used for the extraction of oil and squalene from Amaranthus grain...
  80. pmc Enantioselective inhibition of squalene synthase by aziridine analogues of presqualene diphosphate
    Ali Koohang
    Department of Chemistry, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    J Org Chem 75:4769-77. 2010
    b>Squalene synthase catalyzes the conversion of two molecules of (E,E)-farnesyl diphosphate to squalene via the cyclopropylcarbinyl intermediate, presqualene diphosphate (PSPP)...
  81. pmc Squalene selectively protects mouse bone marrow progenitors against cisplatin and carboplatin-induced cytotoxicity in vivo without protecting tumor growth
    Bikul Das
    Developmental Biology and Stem Cell Program, Research Institute, The Hospital for Sick Children, Toronto, Canada
    Neoplasia 10:1105-19. 2008
    b>Squalene, an isoprenoid antioxidant is a potential cytoprotective agent against chemotherapy-induced toxicity...
  82. pmc Adjuvanted H5N1 vaccine induces early CD4+ T cell response that predicts long-term persistence of protective antibody levels
    Grazia Galli
    Research Center, Novartis Vaccines and Diagnostics, Via Fiorentina 1, Siena 53100, Italy
    Proc Natl Acad Sci U S A 106:3877-82. 2009
    ..We suggest that CD4(+) T cell priming might be used as an early predictor of the immunogenicity of prepandemic vaccines...
  83. ncbi Antifungal activity of bis-azasqualenes, inhibitors of oxidosqualene cyclase
    Samuele Voyron
    Dipartimento di Biologia Vegetale, Universita degli Studi di Torino, Torino, Italy
    Mycoses 53:481-7. 2010
    The antifungal activity and in vitro toxicity toward animal cells of two inhibitors of oxidosqualene cyclase, squalene bis-diethylamine (SBD) and squalene bis-diethylmethylammonium iodide (SBDI) were studied...
  84. ncbi Anticancer efficacy of squalenoyl gemcitabine nanomedicine on 60 human tumor cell panel and on experimental tumor
    L Harivardhan Reddy
    Universite Paris Sud XI, Faculte de Pharmacie, UMR CNRS 8612, IFR 141, 92296 Chatenay Malabry Cedex, France
    Mol Pharm 6:1526-35. 2009
    ..have recently developed the concept of squalenoylation which consisted in the bioconjugation of gemcitabine with squalene, a natural lipid...
  85. ncbi Squalenoylation favorably modifies the in vivo pharmacokinetics and biodistribution of gemcitabine in mice
    L Harivardhan Reddy
    Faculte de Pharmacie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 8612, Institut Fédératif de Recherche 141, Universite Paris XI, Chatenay Malabry Cedex, France
    Drug Metab Dispos 36:1570-7. 2008
    ..Herein, this paper brings comprehensive pharmacokinetic and biodistribution insights that may explain the previously observed greater efficacy of SQdFdC nanoassemblies against experimental leukemia...
  86. ncbi Circadian rhythm of cholesterol synthesis in mouse liver: a statistical analysis of the post-squalene metabolites in wild-type and Crem-knock-out mice
    Jure Acimovic
    Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, Ljubljana, Slovenia
    Biochem Biophys Res Commun 408:635-41. 2011
    ..Here, we show for the first time that intermediates of the post-squalene portion of cholesterol synthesis also follow a circadian rhythm in the mouse liver...
  87. ncbi A linear sesterterpene, two squalene derivatives and two peptide derivatives from Croton hieronymi
    Cesar A N Catalan
    Instituto de Quimica Organica, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Ayacucho 491, 4000 S M de Tucumán, Argentina
    Phytochemistry 64:625-9. 2003
    ..furnished in addition to a large number of plant sterols and triterpenes the C-25 analog of trans-phytol, the squalene derivatives all-trans-2,6,15,19,23-pentamethyltetracosa-2,6,10,(28),14,22,28-hexaene-11-ol and all-trans-10-..
  88. ncbi MF59-adjuvanted vaccine: a safe and useful tool to enhance and broaden protection against seasonal influenza viruses in subjects at risk
    Paolo Durando
    Department of Health Sciences Section of Hygiene and Preventive Medicine, University of Genoa, Head of the Outpatient Clinic of the Hygiene Unit, San Martino University Hospital, Via Pastore 1 16132, Genoa, Italy
    Expert Opin Biol Ther 10:639-51. 2010
    ..The protection offered by non-adjuvanted vaccines may be further reduced by periodic antigenic drifts...
  89. ncbi [Evaluation of alum-adjuvanted whole virus influenza vaccine and future aspects of influenza A (H1N1) 2009 vaccine]
    Toshiaki Ihara
    National Hospital Organization Mie National Hospital, Department of Pediatrics 357 Ohsato Kubota, Tsu, Mie 514 0125, Japan
    Uirusu 60:69-78. 2010
    ..Additionally, since WHO speculates (H1N1) 2009 virus could be endemic in near future, the (H1N1) 2009 virus-derived strain is included in the 2010/11 seasonal influenza vaccine...
  90. ncbi Induction and detection of antibodies to squalene
    G R Matyas
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, MD 20910 7500, USA
    J Immunol Methods 245:1-14. 2000
    ..coated on hydrophobic polyvinyldiene fluoride (PVDF) membranes is described for detecting antibodies that bind to squalene (SQE)...
  91. ncbi Effect of squalene on cyclophosphamide-induced toxicity
    Subramanian Senthilkumar
    Department of Biochemistry, University of Madras, Guindy Campus, Chennai, Tamilnadu 600025, India
    Clin Chim Acta 364:335-42. 2006
    ..Cyclophosphamide (CYP) is a widely used antineoplastic drug, which could cause toxicity of normal cells due to its toxic metabolites. We evaluated the protective role of squalene (SQ) in the toxicity induced by cyclophosphamide.
  92. pmc Improved squalene production via modulation of the methylerythritol 4-phosphate pathway and heterologous expression of genes from Streptomyces peucetius ATCC 27952 in Escherichia coli
    Gopal Prasad Ghimire
    Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, 100 Kalsan ri, Tangjeong myeon, Asansi, Chungnam 336 708, South Korea
    Appl Environ Microbiol 75:7291-3. 2009
    ..hopanoid genes from Streptomyces peucetius were introduced into Escherichia coli to improve the production of squalene, an industrially important compound...
  93. ncbi Squalene-based oil-in-water emulsion adjuvants perturb metabolism of neutral lipids and enhance lipid droplet formation
    Lucie Kalvodova
    Infectious Disease Research Institute, 1124 Columbia St, Suite 400, Seattle, WA 98104, USA
    Biochem Biophys Res Commun 393:350-5. 2010
    ..The accumulated lipids comprise not only the delivered squalene, but also cholesteryl esters, triacylglycerols, fatty acids, and diacylglycerols...
  94. ncbi Induction of CYP3A by 2,3-oxidosqualene:lanosterol cyclase inhibitors is mediated by an endogenous squalene metabolite in primary cultured rat hepatocytes
    Sarita D Shenoy
    Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan 48201, USA
    Mol Pharmacol 65:1302-12. 2004
    ..following agents that inhibit steps upstream of cyclase in the cholesterol biosynthetic pathway: squalestatin 1 (squalene synthase inhibitor), (E)N-ethyl-N-(6,6-dimethyl-2-hepten-4-ynyl)-3-[(3,3'-bithiophen-5-yl)methoxy]..
  95. ncbi Synthesis, characterization, and in vivo delivery of siRNA-squalene nanoparticles targeting fusion oncogene in papillary thyroid carcinoma
    Mouna Raouane
    Laboratoire de Physicochimie, Pharmacotechnie et Biopharmacie, Faculté de Pharmacie, UMR CNRS 8612, Université Paris Sud 11, 5 rue J B Clément, 92296 Châtenay Malabry, France
    J Med Chem 54:4067-76. 2011
    We report the conjugation of the natural lipid squalene (SQ) with a small interfering RNA (siRNA), against the junction oncogene RET/PTC1, usually found in papillary thyroid carcinoma (PTC)...
  96. ncbi Chemopreventive effect of squalene on colon cancer
    C V Rao
    Division of Nutritional Carcinogenesis, American Health Foundation, Valhalla, NY 10595, USA
    Carcinogenesis 19:287-90. 1998
    ..b>Squalene, a constituent of olive oil, and a key intermediate in cholesterol synthesis may be regarded as partially ..
  97. ncbi Cloning and characterization of the Arabidopsis thaliana SQS1 gene encoding squalene synthase--involvement of the C-terminal region of the enzyme in the channeling of squalene through the sterol pathway
    R Kribii
    Laboratoire de Génétique Physiologique et Moléculaire, Institut de Biologie Moléculaire et d Ingénierie Génétique, Universite de Poitiers, France
    Eur J Biochem 249:61-9. 1997
    b>Squalene synthase (SQS) catalyzes the first committed step of the sterol biosynthetic pathway. A full-length Arabidopsis thaliana SQS cDNA has been isolated by combining library screening and PCR-based approaches...
  98. ncbi Yeast oxidosqualene cyclase (Erg7p) is a major component of lipid particles
    Paola Milla
    Dipartimento di Scienza e Tecnologia del Farmaco, Facolta di Farmacia, Universita degli Studi di Torino, Corso Raffaello 31, I 10125 Torino, Italy
    J Biol Chem 277:2406-12. 2002
    ....
  99. ncbi Inhibition of cholesterol synthesis by squalene synthase inhibitors does not induce myotoxicity in vitro
    O P Flint
    Department of Experimental Pathology, Bristol Myers Squibb Pharmaceutical Research Institute, Syracuse, New York 13057, USA
    Toxicol Appl Pharmacol 145:91-8. 1997
    ..Farnesol, geranylgeraniol, and squalene are metabolites of mevalonate...
  100. ncbi Vinyl sulfide derivatives of truncated oxidosqualene as selective inhibitors of oxidosqualene and squalene-hopene cyclases
    M Ceruti
    Dipartimento Farmacochimico, Tossicologico e Biologico, , Italy
    Lipids 36:629-36. 2001
    ..These compounds, having the reactive functions at positions C-2, C-15 and C-19 of the squalene skeleton, were studied as inhibitors of pig liver and Saccharomyces cerevisiae oxidosqualene cyclases (OSC) (EC 5...
  101. ncbi In vitro cytoprotective activity of squalene on a bone marrow versus neuroblastoma model of cisplatin-induced toxicity. implications in cancer chemotherapy
    B Das
    New Agent and Innovative Therapy Program, Division of Hematology and Oncology, The Hospital for Sick Children, Toronto, Canada
    Eur J Cancer 39:2556-65. 2003
    ..Here, we report the in vitro selective cytoprotective activity of squalene, an isoprenoid molecule with antioxidant properties...

Research Grants51

  1. Developing Immunotherapeutics for Methamphetamine Abuse
    THOMAS RICHARD KOSTEN; Fiscal Year: 2010
    ..These adjuvants include CpG oligonucleotides, MPL, and a squalene-based adjuvant;CFA and alum will be included as controls...
  2. Protein Structure/Function by NMR, Crystallography and Computational Chemistry
    Eric Oldfield; Fiscal Year: 2012
    ..In recent work, we discovered that human squalene synthase inhibitors can also block staphyloxanthin biosynthesis in S. aureus, at 200 nM levels. The resulting S...
  3. Regulation of Hepatic P450s by Anti-Cholesterol Drugs
    Thomas A Kocarek; Fiscal Year: 2013
    ..For example, we found that inhibitors of squalene synthase (e.g...
  4. Role of iNOS, Nitric Oxide &Arginase in Statin-Mediated Toxicity in Cancer Cells
    Balaraman Kalyanaraman; Fiscal Year: 2012
    ..their ability to inhibit HMG CoA reductase, which blocks the synthesis of isoprenylated small GTPases, and not by squalene, an immediate precursor of cholesterol...
  5. Human methamphetamine vaccine: Translational Avante Garde Award
    THOMAS RICHARD KOSTEN; Fiscal Year: 2013
    ..protein - Keyhole Limpet Hemocyanin (KLH), which is already approved for human use, and an approved Chinese squalene lipid adjuvant that is better than standard alum to optimize the anti-MA antibody response to vaccination...
  6. Brain Lipid Rafts In Cholesterol Biosynthesis Disorders
    Hongwei Yu; Fiscal Year: 2011
    ..reductase (Dhcr7) deficiencies, because these two models represent disruption of two obligatory pathways of post-squalene cholesterol synthesis...
  7. Characterization of EGCG-receptors: Role in mediating the effects of green tea
    MARK LUNDQUIST; Fiscal Year: 2010
    ..Alternatively, other EGCG-proteins, such as squalene monooxygenase, MICAL, or 67LR might mediate the effects of EGCG in neurons...
  8. SPHINGOLIPIDS AND STEROLS--METABOLISM AND INTERACTIONS
    Seiichi Matsuda; Fiscal Year: 2000
    ..Another hereditary disorder of development, suggested to be a defect in squalene epoxidase, is also being studied...
  9. THE CELLULAR ORGANIZATION OF CHOLESTEROL BIOSYNTHESIS
    Yvonne Lange; Fiscal Year: 1990
    ..will be incubated with 3H-acetate to label biosynthetically so as to induce the accumulation of the intermediates squalene, squalene oxide and lanosterol: (a) incubation at 10 degrees C; (b) treatment with 4,4,10 beta - Trimethyl-trans-..
  10. NST-001 Nanoparticle Formulation for Osmotic pump delivery to inner ear
    Thomas J Lobl; Fiscal Year: 2010
    ..In Phase I and follow-up research, NeuroSystec has successfully developed a squalene-based nanoparticle formulation that addresses the problems with NST-001 formulation...
  11. Synthesis of Macrolides, Steroids, Cyclopentanoids, etc.
    BARRY TROST; Fiscal Year: 2009
    ..to families of compounds possessing powerful antihelmintic and antinematodol mold metabolites ranging to squalene synthase and Ras farnesyl transfer inhibitors...
  12. DRUG RESISTANCE AND GENE AMPLIFICATION IN LEISHMANIA
    Stephen Beverley; Fiscal Year: 2002
    ..genes within the H region for two other drugs of interest to prospective antileishmanial chemotherapy (a squalene epoxidase inhibitor and an 8-aminoquinoline) will be mapped, sequenced and the protein products characterized...
  13. ASYMMETRIC SYNTHESIS OF NOVEL NATURAL PRODUCTS
    MICHAEL CRIMMINS; Fiscal Year: 2006
    ..photocycloadditions applied to a synthesis of the novel natural products CP-263,114 and CP-225,917 which inhibit squalene synthase and ras farnesyl transferase...
  14. MOLECULAR STUDIES OF X-LINKED CHONDRODYSPLASIA PUNCTATA
    Gail Herman; Fiscal Year: 2009
    Seven human disorders involving enzyme defects in post-squalene cholesterol biosynthesis have been identified...
  15. Cytokine-Mediated Regulation of Cholesterogenesis
    Ishaiahu Shechter; Fiscal Year: 2005
    b>Squalene synthase (SQS) catalyzes the first reaction of the isoprenoid metabolic pathway committed to cholesterol biosynthesis and its activity regulates the flux of intermediates to sterols...
  16. NOVEL INHIBITORS OF CHOLESTEROL BIOSYNTHESIS
    Thomas Spencer; Fiscal Year: 1990
    ..Other experiments will be conducted to determine if 24(S),25-epoxycholesterol or other substances derived from squalene dioxide have additional diochemical importance...
  17. SQUALENE AND OXIDOSQUALENE CYCLASES
    Glenn Prestwich; Fiscal Year: 2002
    DESCRIPTION (verbatim from the abstract): Oxidosqualene cyclases (OSCs) and bacterial squalene:hopene cyclases (SHCs) are responsible for generating over 80 different steroidal and other polycyclic triterpene structures...
  18. REGULATION OF ISOPRENOID BIOSYNTHESIS IN RAT LIVER
    RONALD KELLER; Fiscal Year: 1993
    ..that mevalonate derived nonsterol products also affect the branchpoint enzymes trans prenyltransferase and squalene synthase will be examined...
  19. REGULATION OF ENZYMES IN MEVALONATE SYNTHESIS
    HARRY RUDNEY; Fiscal Year: 1991
    ..that there is an alternate pathway for endogenous synthesis of oxysterols which arises from the diversion of squalene 2, 3 monoxide to squalene 2, 3:22,23 diepoxide...
  20. Effect of dietary components on DNA repair
    Michael Wyatt; Fiscal Year: 2004
    ..Phenethyl isothiocyanate (PEITC) and squalene are dietary components that are effective inhibitors of NNK-induced lung tumorigenesis...
  21. SYNTHESIS OF BIOLOGICALLY ACTIVE NATURAL PRODUCTS
    BARRY SNIDER; Fiscal Year: 2009
    ..5) Bisabosqual A inhibits the microsomal squalene syntheses from Saccharomyces cerevisiae, Candida albicans, HepG2 cell and rat liver with IC50 valuesof 0.43, 0...
  22. Natural Product Leads for Drug Development
    KATHLYN PARKER; Fiscal Year: 2009
    ..The bisabosquals are inhibitors of yeast squalene synthase and have potential as leads for the development of antihypercholesteremics...
  23. TOTAL SYNTHESIS OF NATURAL PRODUCTS
    K Nicolaou; Fiscal Year: 2002
    ..The CP-molecules (IVa and IVb) are inhibitors of squalene synthase and farnesyl transferase and are relevant to cardiovascular disease (cholesterol lowering) and cancer ..
  24. Attenuating sterol synthesis in WT and NPC mice brains
    Ta Yuan Chang; Fiscal Year: 2005
    ..We now propose to treat normal and NPC1 mice with a specific cholesterol synthesis inhibitor (squalene synthase inhibitor; SSI) CP-340868 (developed by Pfizer, Inc...
  25. TOTAL SYNTHESIS OF CP-263, 114 AND CP-225, 917
    GARY SULIKOWSKI; Fiscal Year: 2001
    ..compounds, CP-263,114 and CP-225,917, were recently isolated from an unidentified fungus while screening for squalene synthase inhibitors. These natural products were found to inhibit ras farnesyl transferase...
  26. Studies in the Synthesis of Physiologically Important
    John Wood; Fiscal Year: 2006
    ..This proposal describes three such synthetic efforts. The most mature is directed toward a class of squalene synthase inhibitors known as the phomoidrides...
  27. STRUCTURE AND ROLE OF STEROL CARRIER PROTEIN
    Mary Dempsey; Fiscal Year: 1991
    ..In particular, studies will be performed on the multi-functional protein originally called squalene and sterol carrier protein (SCP). One specific aim is to complete the amino acid sequence of rat liver SCP...
  28. ENZYMIC CHOLESTEROL SYNTHESIS
    Mary Dempsey; Fiscal Year: 1980
    The goal of this project is to further basic knowledge in the area of cholesterol biosynthesis and its regulation. In particular, we are purifying and characterizing the squalene and sterol carrier protein.
  29. Sterol Metabolism in Pneumocystis
    EDNA KANESHIRO; Fiscal Year: 2009
    ....
  30. Pneumocystis Biochemistry: Ubiquinones
    EDNA KANESHIRO; Fiscal Year: 2002
    ..carinii CoQ biosynthesis and growth in axenic cultures, and (4) develop atovaquone-resistant P. carinii populations to evaluate CoQ synthesis in sensitive and resistant organisms. ..
  31. NINTH INTERNATIONAL WORKSHOPS ON OPPORTUNISTIC PEOTISTS (IWOP-9)
    EDNA KANESHIRO; Fiscal Year: 2006
    ..As in several IWOP meetings in the past, the 2006 Workshops will be held jointly with the annual meetings of the International Society of Protistologists ( formerly Society of Protozoologists). ..
  32. SPECTROSCOPIC AND RELATED STUDIES ON LENS MEMBRANE LIPID
    Douglas Borchman; Fiscal Year: 2003
    ..An understanding of these alterations will facilitate the development of therapies to prevent or perhaps even reverse these changes. ..
  33. STUDIES IN MOLECULAR RECOGNITION
    Julius Rebek; Fiscal Year: 2010
    ..These cavitands could allow the identification of genes whose expression is influenced by trimethylation of particular lysines of the histones. ..
  34. PHASE IB TRIAL OF CYTOMEGALOVIRUS GLYCOPROTEIN B VACCINE
    Paul Griffiths; Fiscal Year: 2008
    ..If a vaccine schedule can be identified which controls viral load, this will be recommended for a phase II placebo-controlled trial of clinical efficacy. ..
  35. ACUTE RENAL FAILURE--IMPACT OF FLUORINATED ANESTHETICS
    RICHARD ZAGER; Fiscal Year: 2001
    ....
  36. ASSEMBLIES OF SELF COMPLEMENTARY STRUCTURES
    Julius Rebek; Fiscal Year: 2005
    ..We also propose to synthesize new and larger capsules, especially those made up of many identical molecular subunits. ..
  37. SMITH-LEMLI-OPITZ SYNDROME STEROIDOGENESIS
    Cedric Shackleton; Fiscal Year: 2002
    ..Finally, if ring B dehydrosteroids are produced, is the ratio of the individual dehydrometabolites to corresponding conventional metabolites related to the dehydrocholesterol to cholesterol ratio and clinical severity? ..
  38. QUALITATIVE NATURE OF ANTIBODIES TO HCV GLYCOPROTEIN SUBUNIT VACCINE IN HUMANS
    Ranjit Ray; Fiscal Year: 2009
    ..Our proposed complimentary experimental approaches in this vaccinated cohort will help in developing successful vaccine strategies for the intervention of the first crucial step of the HCV life cycle. ..
  39. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..We will concentrate on five enzymes that are potentially enzymes that are potentially excellent drug targets: squalene synthetase, squalene epoxidase, C14 demethylase, delta 14-reductase, and C8 isomerase...
  40. Role of arginine deficiency in pathogenesis of lupus
    Minoru Satoh; Fiscal Year: 2004
    ..abstract_text> ..
  41. Neurosteroids and Smith-Lemli-Optiz Syndrome
    Cedric Shackleton; Fiscal Year: 2005
    ..All studies will require high sensitivity steroid analysis by chemical ionization (CI) GC/MS and HPLC/MS. ..
  42. Functional Activities of HCV Core Protein
    Ranjit Ray; Fiscal Year: 2005
    ....
  43. Gas chromatograph-mass spectrometer with EI and CI
    Cedric Shackleton; Fiscal Year: 2003
    ..g., malondialdehyde) and markers of DNA damage (incorporation of uracil). Dr Kuypers has several projects on the red cell membrane, which require profiling of fatty acids using EI GC/MS. ..
  44. Alphavirus Replicons for Mucosal HIV Vaccines
    Michael Vajdy; Fiscal Year: 2002
    ....
  45. THE YEAST ERGOSTEROL PATHWAY
    Martin Bard; Fiscal Year: 2007
    ..The post-squalene pathway leading to ergosterol in fungi or cholesterol in animal cells share eleven enzymatic reactions in ..
  46. The 17th Annual Conference of the International Society of Exposure of Analysis
    Clifford Weisel; Fiscal Year: 2007
    ..The primary outcome of the meeting will be the advancement of knowledge of human exposure science. [unreadable] [unreadable] [unreadable] [unreadable]..
  47. Iron Therapy in Renal Disease: Potential Toxicities
    RICHARD ZAGER; Fiscal Year: 2008
    ..Potential reasons for these differences will be assessed, with the ultimate goal of defining the safest way of administering parenteral irons to renal disease patients. ..
  48. Characterization of a vaccine against Chlamydia trachomatis
    RHEA COLER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  49. HCV ENVELOPE GLYCOPROTEINS AND VACCINE DEVELOPMENT
    Ranjit Ray; Fiscal Year: 2003
    ....
  50. Antimicrobial Lipids within the Oral Cavity
    PHILIP WESLEY WERTZ; Fiscal Year: 2010
    ....