carbocyanines

Summary

Summary: Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.

Top Publications

  1. ncbi A G-quadruplex ligand 3,3'-diethyloxadicarbocyanine iodide induces mitochondrion-mediated apoptosis but not decrease of telomerase activity in nasopharyngeal carcinoma NPC-TW01 cells
    Chung Pin Li
    Cooperative Laboratory, Cancer Research Division, National Health Research Institutes, Taipei, Taiwan, ROC
    Pharm Res 21:93-100. 2004
  2. pmc Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Mark Bates
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Science 317:1749-53. 2007
  3. pmc In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodies
    Yoshinori Koyama
    Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room 1B40, Bethesda, MD, 20892 1088, USA
    Neoplasia 9:1021-9. 2007
  4. ncbi Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci
    Mandana Veiseh
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Cancer Res 67:6882-8. 2007
  5. ncbi Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells
    James M Flynn
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Mol Vis 12:271-82. 2006
  6. ncbi Evaluation of a proposed in vitro test strategy using neuronal and non-neuronal cell systems for detecting neurotoxicity
    J Gartlon
    ECVAM, European Commission Joint Research Centre, via E Fermi 1, Ispra 21020, Italy
    Toxicol In Vitro 20:1569-81. 2006
  7. doi Accurate distance determination of nucleic acids via Förster resonance energy transfer: implications of dye linker length and rigidity
    Simon Sindbert
    Institut für Physikalische Chemie, Lehrstuhl für Molekulare Physikalische Chemie, Heinrich Heine Universität, Düsseldorf, Germany
    J Am Chem Soc 133:2463-80. 2011
  8. pmc Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrier
    Omid Veiseh
    Departments of Materials Science and Engineering, University of Washington, Seattle, Washington 99195, USA
    Cancer Res 69:6200-7. 2009
  9. ncbi Carbocyanine dyes as efficient reversible single-molecule optical switch
    Mike Heilemann
    Applied Laserphysics and Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 127:3801-6. 2005
  10. ncbi Localized biosensing with Topas microstructured polymer optical fiber
    Grigoriy Emiliyanov
    COM DTU, Department of Communications, Optics and Materials, Technical University of Denmark, Muilding 345v, 2800 Kgs Lyngby, Denmark
    Opt Lett 32:460-2. 2007

Research Grants

  1. STRUCTURAL BASIS OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 1990
  2. Integrated Instrumentation for Proteomics
    Helen Kim; Fiscal Year: 2002
  3. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
  4. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2004
  5. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2006
  6. Molecular Imaging Probes for Reporting on Vascular Oxidative Response
    Alexei A Bogdanov; Fiscal Year: 2010
  7. NOVEL TYROSINASE MUTANTS AS IMAGING MARKER GENES
    Alexei Bogdanov; Fiscal Year: 2002
  8. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2008
  9. Transcription Factor Reporter Technology
    Alexei Bogdanov; Fiscal Year: 2006
  10. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2007

Detail Information

Publications226 found, 100 shown here

  1. ncbi A G-quadruplex ligand 3,3'-diethyloxadicarbocyanine iodide induces mitochondrion-mediated apoptosis but not decrease of telomerase activity in nasopharyngeal carcinoma NPC-TW01 cells
    Chung Pin Li
    Cooperative Laboratory, Cancer Research Division, National Health Research Institutes, Taipei, Taiwan, ROC
    Pharm Res 21:93-100. 2004
    ..In this study, a mitochondrion-mediated apoptotic pathway was demonstrated as another cytotoxic mechanism for DODC action...
  2. pmc Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Mark Bates
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Science 317:1749-53. 2007
    ..Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale...
  3. pmc In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodies
    Yoshinori Koyama
    Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room 1B40, Bethesda, MD, 20892 1088, USA
    Neoplasia 9:1021-9. 2007
    ..Multiple filter sets increase the signal-to-noise ratio by substantially reducing the background signal, and may allow more optical dyes to be resolved within the narrow limits of the near-infrared spectrum...
  4. ncbi Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci
    Mandana Veiseh
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Cancer Res 67:6882-8. 2007
    ..Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies...
  5. ncbi Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells
    James M Flynn
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Mol Vis 12:271-82. 2006
    ....
  6. ncbi Evaluation of a proposed in vitro test strategy using neuronal and non-neuronal cell systems for detecting neurotoxicity
    J Gartlon
    ECVAM, European Commission Joint Research Centre, via E Fermi 1, Ispra 21020, Italy
    Toxicol In Vitro 20:1569-81. 2006
    ....
  7. doi Accurate distance determination of nucleic acids via Förster resonance energy transfer: implications of dye linker length and rigidity
    Simon Sindbert
    Institut für Physikalische Chemie, Lehrstuhl für Molekulare Physikalische Chemie, Heinrich Heine Universität, Düsseldorf, Germany
    J Am Chem Soc 133:2463-80. 2011
    ..A detailed analysis of possible orientation effects (κ(2) problem) indicates that, for short linkers and unknown local environments, additional κ(2)-related uncertainties are clearly outweighed by better defined dye positions...
  8. pmc Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrier
    Omid Veiseh
    Departments of Materials Science and Engineering, University of Washington, Seattle, Washington 99195, USA
    Cancer Res 69:6200-7. 2009
    ....
  9. ncbi Carbocyanine dyes as efficient reversible single-molecule optical switch
    Mike Heilemann
    Applied Laserphysics and Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 127:3801-6. 2005
    ....
  10. ncbi Localized biosensing with Topas microstructured polymer optical fiber
    Grigoriy Emiliyanov
    COM DTU, Department of Communications, Optics and Materials, Technical University of Denmark, Muilding 345v, 2800 Kgs Lyngby, Denmark
    Opt Lett 32:460-2. 2007
    ..The concept is demonstrated using a fluorescence-based method for selective detection of fluorophore-labeled antibodies...
  11. pmc Neurotrophic effects of a cyanine dye via the PI3K-Akt pathway: attenuation of motor discoordination and neurodegeneration in an ataxic animal model
    Hitomi Ohta
    Research Center, Biomedical Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
    PLoS ONE 6:e17137. 2011
    ..In the screening of biologically active molecules for neurotrophic potency, we found that a photosensitizing cyanine dye, NK-4, had remarkable neurotrophic activities and was a potent radical scavenger...
  12. ncbi Accumulation of liposome with Sialyl Lewis X to inflammation and tumor region: application to in vivo bio-imaging
    Masahiko Hirai
    Kobe R and D Center, Katayama Chemical Industries Co, Ltd, Japan
    Biochem Biophys Res Commun 353:553-8. 2007
    ..Thus, it was proven that this liposome is useful not only as an in vivo bio-imaging reagent but also as a drug delivery system (DDS)...
  13. pmc Targeting gelatinases with a near-infrared fluorescent cyclic His-Try-Gly-Phe peptide
    Wei Wang
    Department of Experimental Diagnostic Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Mol Imaging Biol 11:424-33. 2009
    ..The purpose of the study is to synthesize and characterize near-infrared (NIR) fluorescence imaging probes targeted to gelatinases...
  14. doi Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments
    Marcia Levitus
    Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA
    Q Rev Biophys 44:123-51. 2011
    ..These interactions not only affect the photophysical properties of the probes but also create a large uncertainty in their orientation...
  15. ncbi Spontaneous changes in mitochondrial membrane potential in cultured neurons
    J F Buckman
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
    J Neurosci 21:5054-65. 2001
    ..We hypothesize that these DeltaPsi(m) fluctuations are dependent on the proper functioning of the mitochondria and reflect mitochondria alternating between the active and inactive states of oxidative phosphorylation...
  16. ncbi Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
    Judith E Berlier
    Molecular Probes, Inc, Eugene, Oregon 97402, USA
    J Histochem Cytochem 51:1699-712. 2003
    ....
  17. ncbi Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
    Kristen E Adams
    Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030, USA
    J Biomed Opt 12:024017. 2007
    ..5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered...
  18. ncbi Translation factors direct intrinsic ribosome dynamics during translation termination and ribosome recycling
    Samuel H Sternberg
    Department of Chemistry, Columbia University, New York, New York, USA
    Nat Struct Mol Biol 16:861-8. 2009
    ..Our results support the view that specific regulation of the global state of the ribosome is a fundamental characteristic of all translation factors and a unifying theme throughout protein synthesis...
  19. ncbi Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) by using fluorescence-activated flow cytometry
    H David Guthrie
    US Department of Agriculture, Animal Bioscience and Biotechnology Laboratory, Agricultural Research Service, Beltsville, Maryland, USA
    Methods Mol Biol 477:89-97. 2008
    ..We found that the reactive oxygen species generator, menadione reduced sperm motility and reduced Deltapsi(m) in a dose responsive fashion that was closely correlated with the loss of motility...
  20. ncbi Cyanine dyes attenuate cerebral ischemia and reperfusion injury in rats
    Satomi Koya-Miyata
    Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc
    Biol Pharm Bull 33:1872-7. 2010
    ..These results suggest that NK-4 and NK-150 effectively prevented ischemia-induced brain injury through their potent neurotrophin-like activity as well as antioxidative activity...
  21. doi Ribonuclease protection assay on microchip electrophoresis
    Yuka Yamaguchi
    Health Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 2217 14 Hayashi Cho, Takamatsu, 761 0395, Japan
    Analyst 136:2247-51. 2011
    ..These results clearly indicate the potential of microchip electrophoresis for the analysis of mRNA expression in cells...
  22. pmc Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids
    Asif Iqbal
    Cancer Research United Kingdom Nucleic Acid Structure Research Group, MSI WTB complex, The University of Dundee, Dundee DD1 5EH, United Kingdom
    Proc Natl Acad Sci U S A 105:11176-81. 2008
    ..Our data unequivocally establish that Förster transfer obeys the orientation dependence as expected for a dipole-dipole interaction...
  23. pmc Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism
    Qianfan Yang
    Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People s Republic of China
    Nucleic Acids Res 38:1022-33. 2010
    ....
  24. ncbi In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in brain tumor xenografts
    Xiaoyuan Chen
    PET Imaging Science Center, University of Southern California Keck School of Medicine, Los Angeles, California, USA
    Cancer Res 64:8009-14. 2004
    ....
  25. ncbi Heptamethine cyanine dyes with a large stokes shift and strong fluorescence: a paradigm for excited-state intramolecular charge transfer
    Xiaojun Peng
    State Key Laboratory of Fine Chemicals, Dalian University of Technology, 158 Zhongshan Rd, Dalian 116012, PR China
    J Am Chem Soc 127:4170-1. 2005
    ..They were suggested to be a new paradigm for excited-state intramolecular charge transfer (ICT). The configuration change of the bridgehead amine accompanying ICT was investigated in different viscosity and pH media...
  26. ncbi Solid-phase methods for the synthesis of cyanine dyes
    Stephen J Mason
    University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U K
    J Org Chem 70:2939-49. 2005
    ..Overall, both approaches appear to be robust and versatile strategies to delivering a wide range of cyanine-based dyes in high purity...
  27. doi Multi-colour direct STORM with red emitting carbocyanines
    André Lampe
    Institute of Chemistry, Freie Universitat Berlin, 14195 Berlin, Germany
    Biol Cell 104:229-37. 2012
    ....
  28. ncbi Complexation of polymethine dyes with human serum albumin: a spectroscopic study
    Alexander S Tatikolov
    Centro de Quimica Estrutural, Complexo 1, Instituto Superior Tecnico, Av Rovisco Pais, Lisboa 1049 001, Portugal
    Biophys Chem 107:33-49. 2004
    ..Binding to HSA gives rise to dye CD signals and in many cases is accompanied by aggregation of the dyes. These aggregates often exhibit biphasic CD spectra. The aggregates formed by the dyes alone are decomposed in the presence of HSA...
  29. pmc Three color cDNA microarrays: quantitative assessment through the use of fluorescein-labeled probes
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Nucleic Acids Res 31:e14. 2003
    ..80, P < 0.001) was observed. We therefore conclude that slide selection based upon prehybridization quality scores will greatly benefit the ability to generate reliable gene expression data...
  30. ncbi Synthesis, characterization, and biological properties of cyanine-labeled somatostatin analogues as receptor-targeted fluorescent probes
    K Licha
    Institut für Diagnostikforschung GmbH an der Freien Universität Berlin, 14050 Berlin, Germany
    Bioconjug Chem 12:44-50. 2001
    ..In conclusion, receptor-specific peptides are promising tools for designing site-directed optical contrast agents for use in molecular optical imaging...
  31. ncbi Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin
    H J Gruber
    Institute of Biophysics and Institute of Chemistry, J Kepler University, Altenberger Strasse 69, A 4040 Linz, Austria
    Bioconjug Chem 11:696-704. 2000
    ..5, were similar to Cy5. In conclusion the Cy dye series is divided into two groups: Antibodies with multiple Cy3 or Cy3.5 labels yield bright fluorescence while extensive quenching occurs in antibodies labeled with Cy5 and Cy7...
  32. doi Nucleobase-specific enhancement of Cy3 fluorescence
    Billie J Harvey
    Biodesign Institute, Arizona State University, Tempe, AZ 85287 5601, USA
    J Fluoresc 19:443-8. 2009
    ..These results are consistent with a model in which Cy3-nucleoside pi-pi interactions decrease the efficiency of photoisomerization,increasing the efficiency of fluorescence...
  33. pmc Engineered knottin peptides: a new class of agents for imaging integrin expression in living subjects
    Richard H Kimura
    Department of Bioengineering, Cancer Center, Bio X Program, Stanford University, Stanford, California, USA
    Cancer Res 69:2435-42. 2009
    ..Thus, engineered integrin-binding knottin peptides show great potential as clinical diagnostics for a variety of cancers...
  34. pmc Short-range spectroscopic ruler based on a single-molecule optical switch
    Mark Bates
    Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA
    Phys Rev Lett 94:108101. 2005
    ..This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level...
  35. pmc Lack of neurotrophin 3 causes losses of both classes of spiral ganglion neurons in the cochlea in a region-specific fashion
    B Fritzsch
    Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68178, USA
    J Neurosci 17:6213-25. 1997
    ..Despite these impressive innervation deficiencies, the morphology of the organ of Corti and the development of inner and outer hair cells appeared comparatively normal...
  36. ncbi Gene-specific dye bias in microarray reference designs
    Alan A Dombkowski
    Institute of Environmental Health Sciences, Wayne State University, 2727 Second Ave, Detroit, MI 48201, USA
    FEBS Lett 560:120-4. 2004
    ..We demonstrate that this assumption is not reliable and that dye orientation can significantly influence measured changes in gene expression...
  37. pmc Flow cytometric probing of mitochondrial function in equine peripheral blood mononuclear cells
    Dominique Cassart
    Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, B 4000 Liege, Belgium
    BMC Vet Res 3:25. 2007
    ....
  38. ncbi In vivo imaging of experimental arthritis with near-infrared fluorescence
    Andreas Hansch
    Institute of Diagnostic and Interventional Radiology, Fredrich Schiller University Jena, Jena, Germany
    Arthritis Rheum 50:961-7. 2004
    ..To visualize early experimental arthritis with near-infrared fluorescence (NIRF) imaging in a murine model of antigen-induced arthritis (AIA)...
  39. doi False labelling due to quenching failure of N-hydroxy-succinimide-ester-coupled dyes
    Weiqun Wang
    Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, Medical Faculty, University of Munster, Munster, Germany
    Proteomics 10:1525-9. 2010
    ..It could be shown that only a 100-fold increase in the amount of quencher will silence residual dye to such an extent that no artificial signals are detected...
  40. ncbi 1H NMR and optical spectroscopic investigation of the sequence-dependent dimerization of a symmetrical cyanine dye in the DNA minor groove
    Kristen C Hannah
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
    Biochemistry 44:15924-9. 2005
    ....
  41. ncbi Experimental and theoretical study of the interaction of single-stranded DNA homopolymers and a monomethine cyanine dye: nature of specific binding
    Larisa Mikelsons
    Department of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    Photochem Photobiol Sci 4:798-802. 2005
    ....
  42. ncbi Suppressive effects of a cyanine dye against herpes simplex virus (HSV)-1 infection
    Chie Ushio
    Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
    Biomed Res 30:365-8. 2009
    ..These results suggest that lumin exerts an antiviral action against HSV-1 with the independent pathways of IFN-alpha and also it would become a therapeutically effective drug in clinical practice...
  43. ncbi Fluorescence properties and photophysics of the sulfoindocyanine Cy3 linked covalently to DNA
    Matthew E Sanborn
    Department of Chemistry and Biochemistry, Department of Physics and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287 5601, USA
    J Phys Chem B 111:11064-74. 2007
    ..The remarkable variations in the photophysical properties of Cy3-DNA constructs demonstrate that caution should be used when Cy3 is used in studies employing DNA conjugates...
  44. ncbi Nobiletin, a citrus flavonoid, suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cells
    Ai Eguchi
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    FEBS Lett 580:3321-8. 2006
    ..Together, our results suggest that nobiletin is a promising phytochemical for regulating atherosclerosis with reasonable action mechanisms...
  45. ncbi Vessel painting of the microcirculation using fluorescent lipophilic tracers
    Dino J Ravnic
    Laboratory of Immunophysiology, Department of Surgery, Brigham and Women s Hospital, Harvard Surgical Research Laboratories, Harvard Medical School, Room 259, 75 Francis Street, Boston, MA 02115, USA
    Microvasc Res 70:90-6. 2005
    ..These studies suggest the utility of fluorescent vessel painting as a complementary technique to corrosion casting in the morphometric study of the microcirculation...
  46. pmc Model-based analysis of two-color arrays (MA2C)
    Jun S Song
    Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Genome Biol 8:R178. 2007
    ..The tools have been implemented as a stand-alone Java program called MA2C, which can display various plots of statistical analysis for quality control...
  47. pmc Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI
    Yiwen Li
    Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, 506 McKnight Building, 1638 NW 10th Avenue, Miami, Florida 33136, USA
    Nat Protoc 3:1703-8. 2008
    ....
  48. ncbi A new method for in vitro detection of microbially produced mitochondrial toxins
    D Hoornstra
    University of Helsinki, Department of Applied Chemistry and Microbiology, Division of Microbiology, PO Box 56, FIN 00014 University of Helsinki, Finland
    Toxicol In Vitro 17:745-51. 2003
    ..Surfactin and lichenysin A caused mitochondrial damage at concentrations where the plasma membrane was also damaged...
  49. ncbi Kinetic mechanism of direct transfer of Escherichia coli SSB tetramers between single-stranded DNA molecules
    Alexander G Kozlov
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Biochemistry 41:11611-27. 2002
    ..Such a mechanism may be used to recycle SSB tetramers between old and newly formed ssDNA regions during lagging strand DNA replication...
  50. pmc Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements
    William S Branham
    Center for Functional Genomics, Division of Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079, USA
    BMC Biotechnol 7:8. 2007
    ..Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy5 signal relative to the Cy3 signal in 2-color microarrays will adversely reduce the Cy5/Cy3 ratio resulting in unreliable microarray data...
  51. ncbi Chiral transformation of achiral J-aggregates of a cyanine dye templated by human serum albumin
    Yazhou Zhang
    Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P R China
    Chemphyschem 8:224-6. 2007
  52. ncbi Effects of a modified dye-labeled nucleotide spacer arm on incorporation by thermophilic DNA polymerases
    Christopher Lacenere
    Division of Biology, California Institute of Technology, Pasadena, California, USA
    Nucleosides Nucleotides Nucleic Acids 25:9-15. 2006
    ..Furthermore, some of the assayed polymerases are capable of extending the primer to the full or near full length of 30 nucleotides using dye-labeled nucleotides exclusively...
  53. ncbi In vivo imaging of integrin alpha v beta 3 expression using fluorescence-mediated tomography
    Angelika von Wallbrunn
    Department of Clinical Radiology, University of Munster, Albert Schweitzer Strasse 33, 48129, Munster, Germany
    Eur J Nucl Med Mol Imaging 34:745-54. 2007
    ..We therefore synthesised an alpha v beta(3) targeted fluorochrome and imaged tumour xenografts with different alpha v beta(3) expression levels using both planar and tomographic optical imaging methods...
  54. ncbi Intrinsic connectivity of human superior colliculus
    Eric Tardif
    Division de Neuropsychologie, CHUV, 1011, Lausanne, Switzerland
    Exp Brain Res 166:316-24. 2005
    ..The putative roles of these connections are discussed with regard to visual receptive field organization, as well as visuomotor and multisensory integration...
  55. ncbi Comparison of two tricarbocyanine-based dyes for fluorescence optical imaging
    Christin Perlitz
    Optical Imaging and New Modalities Research, Research Laboratories, Schering AG, Müllerstrasse, 13342 Berlin, Germany
    J Fluoresc 15:443-54. 2005
    ..The primary aim was to modify the physicochemical properties of the previously synthesized dye SIDAG to investigate the effect on the in vivo imaging properties...
  56. pmc Triple-target microarray experiments: a novel experimental strategy
    Thorsten Forster
    Scottish Centre for Genomic Technology and Informatics, University of Edinburgh, The Chancellor s Building, College of Medicine, 49 Little France Crescent, Edinburgh, EH16 4SB, UK
    BMC Genomics 5:13. 2004
    ....
  57. ncbi Role reversal in a supramolecular assembly: a chiral cyanine dye controls the helicity of a peptide-nucleic acid duplex
    Babu Rao Renikuntla
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
    Langmuir 21:5362-6. 2005
    ..These results indicate that chirality is transferred from the dye to the PNA, as opposed to other examples of polymer-templated dye aggregation where chirality is transferred from the template to the dye...
  58. ncbi Olivary projection from the rostral part of the nucleus of Darkschewitsch in the postnatal rat as revealed through the use of a carbocyanine dye
    Satoru Onodera
    Department of Anatomy, School of Medicine, Iwate Medical University, Uchimaru 19 1, Morioka 020 8505, Japan
    Brain Res 1015:194-7. 2004
    ..The present study suggests the existence of a precise topographic organization from subnuclei of the area parafascicularlis prerublaris to subnuclei of the inferior olive...
  59. ncbi Nonblinking and long-lasting single-molecule fluorescence imaging
    Ivan Rasnik
    Department of Physics, Emory University, Atlanta, Georgia 30322, USA
    Nat Methods 3:891-3. 2006
    ....
  60. ncbi Effect of PNA backbone modifications on cyanine dye binding to PNA-DNA duplexes investigated by optical spectroscopy and molecular dynamics simulations
    Isil Dilek
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    J Am Chem Soc 127:3339-45. 2005
    ....
  61. pmc Intensity-based analysis of two-colour microarrays enables efficient and flexible hybridization designs
    Peter A C 't Hoen
    Center for Human and Clinical Genetics, Leiden University Medical Center, The Netherlands
    Nucleic Acids Res 32:e41. 2004
    ....
  62. ncbi A multimodal nanoparticle for preoperative magnetic resonance imaging and intraoperative optical brain tumor delineation
    Moritz F Kircher
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Cancer Res 63:8122-5. 2003
    ..This prototypical multimodal nanoparticle has unique properties that may allow radiologists and neurosurgeons to see the same probe in the same cells and may offer a new approach for obtaining tumor margins...
  63. pmc Optimization of oligonucleotide-based DNA microarrays
    Angela Relógio
    Gene Expression Programme and Functional Genomics Technology and Instrumentation Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nucleic Acids Res 30:e51. 2002
    ..These data will facilitate the design and standardization of custom-made microarrays applicable to gene expression profiling and sequencing analyses...
  64. ncbi Near-infrared fluorescent nanoparticles as combined MR/optical imaging probes
    Lee Josephson
    Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown 02129, USA
    Bioconjug Chem 13:554-60. 2002
    ..The probes may provide the basis for a new class of so-called smart nanoparticles, capable of pinpointing their position through their magnetic properties, while providing information on their environment by optical imaging techniques...
  65. doi Fluorescent probes for super-resolution imaging in living cells
    Marta Fernandez-Suarez
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Rev Mol Cell Biol 9:929-43. 2008
    ..We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development...
  66. pmc Robust and efficient synthetic method for forming DNA microarrays
    P L Dolan
    Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
    Nucleic Acids Res 29:E107-7. 2001
    ..Thus, the ATMS/diazotization chemistry facilitated covalent binding of unmodified DNA, and the reusable microarrays that were produced showed enhanced levels of hybridization and very low background fluorescence...
  67. ncbi G-quadruplex DNA binding by a series of carbocyanine dyes
    S M Kerwin
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, 78712, USA
    Bioorg Med Chem Lett 11:2411-4. 2001
    ..Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration...
  68. doi Live imaging of mammalian retina: rod outer segments are stained by conventional mitochondrial dyes
    Paolo Bianchini
    University of Genoa, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy LAMBS, MicroSCoBiO Research Center, Department of Physics, Via Dodecaneso 33, 16146, Genoa, Italy
    J Biomed Opt 13:054017. 2008
    ..This finding may shed light on previous reports of the existence of a proton potential across the disk membranes and on the mechanism of the adenosine tri-phosphate (ATP) supply for phototransduction, which still requires investigation...
  69. ncbi Investigation of DNA binding modes for a symmetrical cyanine dye trication: effect of DNA sequence and structure
    R Cao
    Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213-3890, USA
    J Biomol Struct Dyn 18:844-56. 2001
    ..Seifert, J.L., et al. (1999) J. Am. Chem. Soc. 121, 2987-2995] These studies illustrate the diversity of DNA binding modes that are available to a given ligand structure...
  70. ncbi DNA sequence-dependent enhancement of Cy3 fluorescence
    Billie Jo Harvey
    Biodesign Institute, Arizona State University, Arizona State University, Tempe, AZ, USA
    Photochem Photobiol Sci 8:1105-10. 2009
    ..We have shown that the ability of Cy3 to interact with DNA depends on the flexibility of the oligonucleotide and the presence of purines in the chain...
  71. ncbi Multiplex detection and quantitation of proteins on western blots using fluorescent probes
    J C Gingrich
    Bio Rad Laboratories, Hercules, CA, USA
    Biotechniques 29:636-42. 2000
    ..Two examples of three-color multiplex detection using FITC-, Cy3- and Cy5-conjugated probes on Western blots are provided to demonstrate applications of this approach...
  72. ncbi Location of cyanine-3 on double-stranded DNA: importance for fluorescence resonance energy transfer studies
    D G Norman
    Department of Biochemistry, The University of Dundee, UK
    Biochemistry 39:6317-24. 2000
    ..The knowledge of the fluorophore positions can now be used for more precise interpretation of FRET data from nucleic acids...
  73. ncbi The combined effects of trkB and trkC mutations on the innervation of the inner ear
    B Fritzsch
    Creighton University, Department of Biomedical Sciences, Omaha, NE 68178, USA
    Int J Dev Neurosci 16:493-505. 1998
    ....
  74. pmc Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes
    J B Randolph
    Center for Light Microscope Imaging and Biotechnology and Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Nucleic Acids Res 25:2923-9. 1997
    ..A mechanism that accounts for this destabilization is proposed which is consistent with our data. It involves dye-dye and dye-nucleotide interactions which appear to stabilize a single-stranded conformation of the probe...
  75. doi Chiral transformation of cyanine dye aggregates induced by small peptides
    Qianfan Yang
    Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry for Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, People s Republic of China
    J Phys Chem B 112:8783-7. 2008
    ..This might be the reason for cyanine dye to form the different chiral assembly induced by these oligo-peptide templates...
  76. pmc An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments
    Colin Echeverría Aitken
    Biophysics Program, Stanford University School of Medicine, Stanford, California 94305 5126, USA
    Biophys J 94:1826-35. 2008
    ..We observed increased stability of Cy3 and Alexa488 in the presence of the antioxidants ascorbic acid and n-propyl gallate. This new O(2)-scavenging system should have wide application for single-molecule fluorescence experiments...
  77. ncbi Effects of atmospheric ozone on microarray data quality
    Thomas L Fare
    Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA
    Anal Chem 75:4672-5. 2003
    ..To address this environmental effect, laboratory ozone levels should be kept below 2 ppb (e.g., with filters in HVAC) to achieve high quality microarray data...
  78. ncbi Comparison of Alexa Fluor and CyDye for practical DNA microarray use
    Joanne L Ballard
    Lotterywest State Microarray Facility, School of Medicine and Pharmacology, The University of Western Australia and Centre for Medicinal Research, Western Australian Institute for Medical Research, Nedlands, WA, Australia
    Mol Biotechnol 36:175-83. 2007
    ..These results suggest that precautions against potential dye affects will continue to be necessary and that no one dye pair negates this need...
  79. pmc Fluorescent labelling of cRNA for microarray applications
    Peter A C 't Hoen
    Center for Human and Clinical Genetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
    Nucleic Acids Res 31:e20. 2003
    ..In conclusion, the currently developed method is an efficient, robust and inexpensive technique for fluorescent labelling of cRNA and allows sensitive detection of gene expression profiles on oligonucleotide microarrays...
  80. pmc Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Anal Biochem 315:57-66. 2003
    ..These results suggest that maximum fluorescence enhancements occur about 90A from the silver surface, a distance readily obtained by one or two layers of proteins...
  81. pmc Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particles
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 317:136-46. 2003
    ..These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays...
  82. ncbi Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles
    Joanna Malicka
    Department of Chemistry, University of Gdansk, 80 952 Gdansk, Sobieskiego 18, Poland
    Biopolymers 72:96-104. 2003
    ..These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression...
  83. ncbi Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
    V N Dedov
    Department of Pharmacy, University of Sydney, NSW, 2006, Syndey, Australia
    Micron 32:653-60. 2001
    ....
  84. ncbi Real-time detection of nucleotide incorporation during complementary DNA strand synthesis
    Alexander Krieg
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chembiochem 4:589-92. 2003
    ..The efficiency of this reaction is of the same order of magnitude as that of a bimolecular hybridization reaction...
  85. ncbi Development of improved cell lysis, solubilization and imaging approaches for proteomic analyses
    Richard M Leimgruber
    Pharmacia, Discovery Research and Development, St Louis, MO 63198, USA
    Proteomics 2:135-44. 2002
    ..Using these approaches, it is possible to perform more rapid and accurate comparative analyses and proteomic, post-gel characterization of proteins of interest than using comparative image analysis of multiple gels...
  86. ncbi DNA-templated assembly of helical cyanine dye aggregates: a supramolecular chain polymerization
    Kristen C Hannah
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Acc Chem Res 37:845-53. 2004
    ..This Account describes how polymerization depends on the dye structure and DNA sequence and also summarizes the interesting optical properties exhibited by these chiral, helical materials...
  87. ncbi Fast detection of single nucleotide polymorphisms (SNPs) by primer elongation with monitoring of supercritical-angle fluorescence
    Alexander Krieg
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Chembiochem 5:1680-5. 2004
    ..We demonstrate the efficiency of the method by detecting an SNP in the ErbB2 gene that is involved in causing a higher risk of breast cancer...
  88. ncbi The use of CalciumOrange-5N as a specific marker of mitochondrial Ca2+ in mouse skeletal muscle fibers
    Pura Bolaños
    Laboratorio de Fisiologia Celular, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas IVIC, Apartado 21827, Caracas 1020A, Venezuela
    Pflugers Arch 455:721-31. 2008
    ..Our results show the loss of Ca(2+) from mitochondria when DeltaPsi(m) is depolarised and demonstrate the usefulness of CaOr-5N to mark mitochondrial [Ca(2+)](m)...
  89. ncbi Influence of co-down-regulation of caspase-3 and caspase-7 by siRNAs on sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin
    Yun Hee Sung
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Kusong Dong, Yusong gu, Daejon 305 701, Republic of Korea
    Metab Eng 9:452-64. 2007
    ..Taken together, co-down-regulation of caspase-3 and caspase-7 is effective in regard to extension of culture longevity and enhancement of hTPO production in a serum-free culture in the presence or absence of 1mM NaBu addition...
  90. ncbi Studies of monomeric and homodimeric oxazolo[4,5-b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization
    V B Kovalska
    Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, 150 Zabolotnogo St, 03143 Kyiv, Ukraine
    J Biochem Biophys Methods 68:155-65. 2006
    ..8 ng of dsdna...
  91. ncbi Anti-cancer effect of resveratrol is associated with induction of apoptosis via a mitochondrial pathway alignment
    Weimin Sun
    Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Adv Exp Med Biol 614:179-86. 2008
    ....
  92. ncbi P53 mediated regulation of metallothionein transcription in breast cancer cells
    Elena A Ostrakhovitch
    Department of Pathology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Cell Biochem 102:1571-83. 2007
    ..On the contrary, MRE activity remained unaltered in MDD2 cells with inactive p53. The above results demonstrate that activation of p53 is an important factor in metal regulation of MT...
  93. ncbi Psychosine-induced apoptosis and cytokine activation in immune peripheral cells of Krabbe patients
    Patrizia Formichi
    Department of Neurological and Behavioural Sciences, University of Siena, Siena, Italy
    J Cell Physiol 212:737-43. 2007
    ..In conclusion, the reduced apoptotic response and the atypical cytokine production observed in our experiments, suggest an involvement of inflammatory pattern in immune peripheral cells of KP...
  94. pmc Plasmonic enhancement of single-molecule fluorescence near a silver nanoparticle
    Yi Fu
    Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 W Lombard Street, Baltimore, MD 21201, USA
    J Fluoresc 17:811-6. 2007
    ..These results demonstrate that the use of fluorophore-metal interactions can increase the brightness and photostability of fluorophores for single molecule detection...
  95. ncbi The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells
    Justin C St John
    Mitochondrial Reproductive Genetics Group, Division of Medical Sciences, The Medical School, University of Birmingham, UK
    Methods Mol Biol 331:347-74. 2006
    ..Last, we describe real-time polymerase chain reaction protocols that allow both the identification of mtDNA copy number and determine whether mtDNA copy is uniform (homoplasmy) in its transmission or heterogeneous (heteroplasmy)...
  96. doi In vivo imaging of the inflammatory receptor CD40 after cerebral ischemia using a fluorescent antibody
    Jan Klohs
    Department Experimental Neurology, Charite University Medicine Berlin, Berlin, Germany
    Stroke 39:2845-52. 2008
    ..5 (cy5.5-cd40mab)...
  97. ncbi A new optical platform for biosensing based on fluorescence anisotropy
    F Baldini
    Institute of Applied Physics, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino FI, Italy
    Anal Bioanal Chem 391:1837-44. 2008
    ..The treatment with Eudragit L100 was found to be the most successful. Limits of detection and quantification of 0.05 microg mL(-1) and 0.2 microg mL(-1), respectively, were obtained with the configuration described...
  98. ncbi Role of wild-type estrogen receptor-beta in mitochondrial cytoprotection of cultured normal male and female human lens epithelial cells
    J M Flynn
    Department of Cell Biology, University of North Texas Health Science Center, Fort Worth, TX, USA
    Am J Physiol Endocrinol Metab 295:E637-47. 2008
    ..Thus, whereas the estrogen-mediated prevention of mitochondrial membrane permeability transition is sex independent, the mechanism of estrogen-induced mitochondrial cytoprotection is wtERbeta1 dependent...
  99. ncbi JC-1, a sensitive probe for a simultaneous detection of P-glycoprotein activity and apoptosis in leukemic cells
    Driss Chaoui
    Universite Pierre et Marie Curie Paris 6, UMR S 736, Les Cordeliers, France
    Cytometry B Clin Cytom 70:189-96. 2006
    ..Therefore, we wanted to see if JC-1 could also simultaneously assess both, P-gp activity and apoptosis, in acute myeloid leukemia (AML) cells...
  100. ncbi A selective method for transfection of retinal ganglion cells by retrograde transfer of antisense oligonucleotides against kynurenine aminotransferase II
    Sebastian Thaler
    Department of Pathophysiology of Vision and Neuro ophthalmology, University Eye Hospital, Tubingen, Germany
    Mol Vis 12:100-7. 2006
    ..Expression of kynurenine aminotransferase II (KAT II) in RGCs has been well described in the literature. We describe a new method for downregulating cellular KAT II expression via transfection of RGC by retrograde transfer of ODN...
  101. ncbi Exogenous phospholipids specifically affect transmembrane potential of brain mitochondria and cytochrome C release
    Lucia Piccotti
    Department of Internal Medicine, Laboratory of Biochemistry, University of Perugia, Perugia 06122, Italy
    J Biol Chem 277:12075-81. 2002
    ..Moreover, enrichment with CL and PG brought the low energy mitochondrial population to high Deltapsi(m) values and prevented phosphate-dependent cytochrome c release...

Research Grants108 found, 100 shown here

  1. STRUCTURAL BASIS OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 1990
    ..Improving assessment of these functions in hearing impaired individuals requires experiments such as the one proposed to increase our knowledge about the anatomical and physiological basis for neural processing of sound...
  2. Integrated Instrumentation for Proteomics
    Helen Kim; Fiscal Year: 2002
    ....
  3. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
    ..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
  4. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2004
    ....
  5. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2006
    ....
  6. Molecular Imaging Probes for Reporting on Vascular Oxidative Response
    Alexei A Bogdanov; Fiscal Year: 2010
    ....
  7. NOVEL TYROSINASE MUTANTS AS IMAGING MARKER GENES
    Alexei Bogdanov; Fiscal Year: 2002
    ..The long-term goal of this research is to explore and develop novel and useful bifunctional "imaging/therapeutic marker genes" for in vivo use. ..
  8. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2008
    ..These tools will have applications for tracking the molecules that are linked to the abnormal cells. This research will help scientists and physicians to detect these cells and follow their response to medicines. ..
  9. Transcription Factor Reporter Technology
    Alexei Bogdanov; Fiscal Year: 2006
    ..abstract_text> ..
  10. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2007
    ..These tools will have applications for tracking the molecules that are linked to the abnormal cells. This research will help scientists and physicians to detect these cells and follow their response to medicines. ..
  11. Molecular Imaging to Determine the Risk of Rupture of Cerebral Aneurysms
    Alexei Bogdanov; Fiscal Year: 2008
    ..This can be done by using magnetic resonance imaging in patients without the need of opening the skull. Then this group of patients could be treated before aneurysm bursts and cause a severe stroke. ..
  12. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2005
    ....
  13. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2005
    ....
  14. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2009
    ..These tools will have applications for tracking the molecules that are linked to the abnormal cells. This research will help scientists and physicians to detect these cells and follow their response to medicines. ..
  15. MR SIGNAL AMPLIFICATION FOR RECEPTOR IMAGING
    Alexei Bogdanov; Fiscal Year: 2003
    ....
  16. Annual Meeting of the NCRR/NIBIB Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2006
    ..5. Poster sessions and small focus groups arranged by the Principal Investigators, and 6. A closing session without NIH personnel to elect organizing committee and other discussions. ..
  17. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2006
    ..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
  18. CENTER FOR FLUORESCENCE SPECTROSCOPY
    Joseph Lakowicz; Fiscal Year: 2006
    ..Abstract Not Provided. ..
  19. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2008
    ..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
  20. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2005
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  21. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2005
    ..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
  22. HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITS
    CRAIG HENKEL; Fiscal Year: 2005
    ....
  23. Dissection of cellular interactions in T1DM with integrated functional genomics
    MARTIN JOHN HESSNER; Fiscal Year: 2010
    ....
  24. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2004
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  25. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2004
    ..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
  26. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2004
    ..3) Assess expression of the lesion and test efficacy of protective metabolites during ischemia/reperfusion of the kidney in vivo. ..
  27. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2004
    ..Both one-photon and multi-photon excitation will be used. We expect this project to determine how metallic particle effects on fluorescence can be used for new approaches to DNA analysis. ..
  28. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2004
    ..a. Develop sandwich (capture) immunoassays for PSA using anti-PSA (or anti-ACT-PSA) labeled with low and high quantum yield fluorophores. b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. ..
  29. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2006
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  30. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel M Weinberg; Fiscal Year: 2010
    ....
  31. Molecular and Microstructural Imaging of Cardiomyocyte Apoptosis and Autophagy
    David E Sosnovik; Fiscal Year: 2010
    ..The development of such strategies has the potential to significantly reduce the rapidly rising number of patients with heart failure, and is thus of major clinical and public health significance. ..
  32. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2009
    ..This extension of the specific aims will result in the hiring of two PhD-level research associates. ..
  33. Molecular and Microstructural Imaging of Cardiomyocyte Apoptosis and Autophagy
    David Sosnovik; Fiscal Year: 2009
    ..The development of such strategies has the potential to significantly reduce the rapidly rising number of patients with heart failure, and is thus of major clinical and public health significance. ..
  34. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2009
    ..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
  35. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2009
    ..B. Apply the nanostructures array for identification of class B biohazard agents. C and D. Apply the nanostructures for analysis of the cystic fibrosis transmembrane regulator gene (CFTR). ..
  36. Dissection of cellular interactions with integrated functional genomics in comple
    Martin Hessner; Fiscal Year: 2008
    ..Understanding the relationship between mast cells, T effector, and TREG cells in autoimmunity and has the potential to establish a new paradigm for immune regulation and create new insights to human disease. ..
  37. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2009
    ....
  38. Dissection of cellular interactions in T1DM with integrated functional genomics
    Martin Hessner; Fiscal Year: 2009
    ....
  39. Molecular and Microstructural Imaging of Cardiomyocyte Apoptosis and Autophagy
    David Sosnovik; Fiscal Year: 2008
    ..The development of such strategies has the potential to significantly reduce the rapidly rising number of patients with heart failure, and is thus of major clinical and public health significance. ..
  40. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2007
    ..abstract_text> ..
  41. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2007
    ..B. Apply the nanostructures array for identification of class B biohazard agents. C and D. Apply the nanostructures for analysis of the cystic fibrosis transmembrane regulator gene (CFTR). ..
  42. Plasmon-Controlled Fluorescence and Cardiac Markers
    Joseph Lakowicz; Fiscal Year: 2007
    ..Cardiac troponin I and BMP are present at lower concentrations and their detection will drive development of nanostructures for high sensitivity detection. ..
  43. Magnetic Resonance Imaging of Cardiomyocyte Apoptosis
    David Sosnovik; Fiscal Year: 2007
    ..The proposed research also has the potential to facilitate a greater understanding of cardiomyocyte apoptosis and accelerate the development of novel cardioprotective strategies. ..
  44. Metallic Surfaces and Particles in DNA Analysis
    Joseph R Lakowicz; Fiscal Year: 2010
    ..Each type of structure provides unique opportunities for new assay formats and can be readily available to other laboratories at low cost. ..
  45. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 1992
    ..The studies in this proposal provide an established and versatile approach for achieving this...
  46. STRUCTURE AND PLASTICITY OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 2002
    ..Results should lead to a better understanding of the influence of experience on the development of these binaural sensory pathways. ..
  47. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2000
    ..dependent, disassembly/reassembly of focal adhesions in the glycine-protected, isolated tubules. ..
  48. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2001
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  49. STRUCTURE AND PLASTICITY OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 2001
    ..Results should lead to a better understanding of the influence of experience on the development of these binaural sensory pathways. ..
  50. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2001
    ..3) Assess expression of the lesion and test efficacy of protective metabolites during ischemia/reperfusion of the kidney in vivo. ..
  51. HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITS
    CRAIG HENKEL; Fiscal Year: 2002
    ....
  52. HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITS
    CRAIG HENKEL; Fiscal Year: 2001
    ....
  53. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 1991
    ..The studies in this proposal provide an established and versatile approach for achieving this...
  54. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 1990
    ..The studies in this proposal provide an established and versatile approach for achieving this...
  55. STRUCTURE AND PLASTICITY OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 1999
    ..Results should lead to a better understanding of the influence of experience on the development of these binaural sensory pathways. ..
  56. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 1999
    ..dependent, disassembly/reassembly of focal adhesions in the glycine-protected, isolated tubules. ..
  57. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 1993
    ..Glycine cytoprotection is an extraordinarily powerful tool for this purpose with implications for injury to the kidney and other organs..
  58. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2002
    ..3) Assess expression of the lesion and test efficacy of protective metabolites during ischemia/reperfusion of the kidney in vivo. ..
  59. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2002
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  60. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2002
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  61. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2003
    ..3) Assess expression of the lesion and test efficacy of protective metabolites during ischemia/reperfusion of the kidney in vivo. ..
  62. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2003
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  63. Biohazard Detection Using Metal-Enhanced Fluorescence
    Joseph Lakowicz; Fiscal Year: 2003
    ..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
  64. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2003
    ..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
  65. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2003
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  66. HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITS
    CRAIG HENKEL; Fiscal Year: 2004
    ....
  67. Metallic Surfaces and Particles in DNA Analysis
    Joseph Lakowicz; Fiscal Year: 2003
    ..Both one-photon and multi-photon excitation will be used. We expect this project to determine how metallic particle effects on fluorescence can be used for new approaches to DNA analysis. ..
  68. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2004
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  69. HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITS
    CRAIG HENKEL; Fiscal Year: 2003
    ....
  70. Solvent exchange methods for protein microencapsulation
    Kinam Park; Fiscal Year: 2004
    ..Because of the simplicity of the solvent exchange method, it is expected to open a new avenue of microencapsulation of protein drugs with various properties. ..
  71. Genetic remodeling of Vagal Afferent Organization
    Edward Fox; Fiscal Year: 2009
    ..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
  72. Solvent exchange methods for protein microencapsulation
    Kinam Park; Fiscal Year: 2005
    ..Because of the simplicity of the solvent exchange method, it is expected to open a new avenue of microencapsulation of protein drugs with various properties. ..
  73. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2003
    ..This will allow more effective use of existing drugs and development of various new drug candidates that are otherwise eliminated from further consideration due to their poor water-solubilities. ..
  74. Microenvironment-Controlled Encapsulation (MICE) Process
    Kinam Park; Fiscal Year: 2005
    ..The MICE process will facilitate microencapsulation of a variety of protein drugs that have been difficult to formulate with the conventional methods, as well as small molecular weight drugs. ..
  75. Engineering Microbial Rhodopsins as Optical Voltage Sensors
    ADAM EZRA COHEN; Fiscal Year: 2011
    ..This protein will facilitate studies on the electrophysiology of a wide range of cells implicated in human health and disease. ..
  76. Adaptable Polymer Micelles for Tumor Targeting
    Kinam Park; Fiscal Year: 2011
    ..e., adaptable polymer micelles) for targeted delivery of anticancer agents to the tumor site via intravenous injection. ..
  77. Rapid ischemic tolerance: Synaptic re-organization and reduced excitotoxicity
    Robert Meller; Fiscal Year: 2009
    ..It is our aim to investigate these mechanisms to help develop new therapeutics for stroke, or for circumstances where ischemia can be predicted, such as heart bypass surgery. ..
  78. Rapid ischemic tolerance: Synaptic re-organization and reduced excitotoxicity
    Robert Meller; Fiscal Year: 2010
    ..It is our aim to investigate these mechanisms to help develop new therapeutics for stroke, or for circumstances where ischemia can be predicted, such as heart bypass surgery. ..
  79. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2004
    ..This will allow more effective use of existing drugs and development of various new drug candidates that are otherwise eliminated from further consideration due to their poor water-solubilities. ..
  80. Adaptable Polymer Micelles for Tumor Targeting
    Kinam Park; Fiscal Year: 2010
    ..e., adaptable polymer micelles) for targeted delivery of anticancer agents to the tumor site via intravenous injection. ..
  81. Solvent exchange methods for protein microencapsulation
    Kinam Park; Fiscal Year: 2003
    ..Because of the simplicity of the solvent exchange method, it is expected to open a new avenue of microencapsulation of protein drugs with various properties. ..
  82. Solvent exchange methods for protein microencapsulation
    Kinam Park; Fiscal Year: 2006
    ..Because of the simplicity of the solvent exchange method, it is expected to open a new avenue of microencapsulation of protein drugs with various properties. ..
  83. Protein ubiquitination based neuroprotection for stroke
    Robert Meller; Fiscal Year: 2006
    ....
  84. Genetic remodeling of Vagal Afferent Organization
    Edward Fox; Fiscal Year: 2007
    ..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
  85. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2007
    ..The oral paclitaxel formulations are expected to have great therapeutic potential not only for cancer treatment as well as neurodegenerative diseases that can be potentially treated by paclitaxel. [unreadable] [unreadable]..
  86. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2002
    ..This will allow more effective use of existing drugs and development of various new drug candidates that are otherwise eliminated from further consideration due to their poor water-solubilities. ..
  87. Layer-by-layer assembly for making drug-eluting stents
    Kinam Park; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  88. Genetic remodeling of Vagal Afferent Organization
    Edward Fox; Fiscal Year: 2006
    ..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
  89. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2006
    ..The oral paclitaxel formulations are expected to have great therapeutic potential not only for cancer treatment as well as neurodegenerative diseases that can be potentially treated by paclitaxel. [unreadable] [unreadable]..
  90. Genetic remodeling of Vagal Afferent Organization
    Edward Fox; Fiscal Year: 2005
    ..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
  91. Rapid ischemic tolerance: Ubiquitin-mediated structural reorganization
    Robert Meller; Fiscal Year: 2007
    ..Indeed, the long-term aim of these studies is to discover endogenous protective mechanisms that can be translated into effective rapid acting neuroprotective agents for stroke. [unreadable] [unreadable] [unreadable]..
  92. Layer-by-layer assembly for making drug-eluting stents
    Kinam Park; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  93. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2008
    ..The oral paclitaxel formulations are expected to have great therapeutic potential not only for cancer treatment as well as neurodegenerative diseases that can be potentially treated by paclitaxel. ..
  94. Genetic remodeling of Vagal Afferent Organization
    Edward Fox; Fiscal Year: 2008
    ..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
  95. Magnetic Resonance Imaging of Cardiomyocyte Apoptosis
    David Sosnovik; Fiscal Year: 2008
    ..The proposed research also has the potential to facilitate a greater understanding of cardiomyocyte apoptosis and accelerate the development of novel cardioprotective strategies. ..