carbocyanines

Summary

Summary: Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.

Top Publications

  1. ncbi A G-quadruplex ligand 3,3'-diethyloxadicarbocyanine iodide induces mitochondrion-mediated apoptosis but not decrease of telomerase activity in nasopharyngeal carcinoma NPC-TW01 cells
    Chung Pin Li
    Cooperative Laboratory, Cancer Research Division, National Health Research Institutes, Taipei, Taiwan, ROC
    Pharm Res 21:93-100. 2004
  2. pmc Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Mark Bates
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Science 317:1749-53. 2007
  3. ncbi Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments
    Marcia Levitus
    Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA
    Q Rev Biophys 44:123-51. 2011
  4. pmc Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrier
    Omid Veiseh
    Departments of Materials Science and Engineering, University of Washington, Seattle, Washington 99195, USA
    Cancer Res 69:6200-7. 2009
  5. ncbi Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci
    Mandana Veiseh
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Cancer Res 67:6882-8. 2007
  6. ncbi Evaluation of a proposed in vitro test strategy using neuronal and non-neuronal cell systems for detecting neurotoxicity
    J Gartlon
    ECVAM, European Commission Joint Research Centre, via E Fermi 1, Ispra 21020, Italy
    Toxicol In Vitro 20:1569-81. 2006
  7. ncbi Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells
    James M Flynn
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Mol Vis 12:271-82. 2006
  8. pmc Photoswitching mechanism of cyanine dyes
    Graham T Dempsey
    Graduate Program in Biophysics, School of Engineering and Applied Sciences, Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Am Chem Soc 131:18192-3. 2009
  9. ncbi Spontaneous changes in mitochondrial membrane potential in cultured neurons
    J F Buckman
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
    J Neurosci 21:5054-65. 2001
  10. pmc Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging
    Richa Dave
    Tri Institutional Program in Chemical Biology, Weill Cornell Medical College of Cornell University, New York, New York, USA
    Biophys J 96:2371-81. 2009

Research Grants

  1. Integrated Instrumentation for Proteomics
    Helen Kim; Fiscal Year: 2002
  2. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
  3. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2005
  4. NOVEL TYROSINASE MUTANTS AS IMAGING MARKER GENES
    Alexei Bogdanov; Fiscal Year: 2002
  5. Transcription Factor Reporter Technology
    Alexei Bogdanov; Fiscal Year: 2006
  6. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2007
  7. Biohazard Detection Using Metal-Enhanced Fluorescence
    Joseph Lakowicz; Fiscal Year: 2003
  8. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2006
  9. STRUCTURE AND PLASTICITY OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 2002
  10. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2006

Detail Information

Publications246 found, 100 shown here

  1. ncbi A G-quadruplex ligand 3,3'-diethyloxadicarbocyanine iodide induces mitochondrion-mediated apoptosis but not decrease of telomerase activity in nasopharyngeal carcinoma NPC-TW01 cells
    Chung Pin Li
    Cooperative Laboratory, Cancer Research Division, National Health Research Institutes, Taipei, Taiwan, ROC
    Pharm Res 21:93-100. 2004
    ..In this study, a mitochondrion-mediated apoptotic pathway was demonstrated as another cytotoxic mechanism for DODC action...
  2. pmc Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Mark Bates
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Science 317:1749-53. 2007
    ..Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale...
  3. ncbi Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments
    Marcia Levitus
    Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA
    Q Rev Biophys 44:123-51. 2011
    ..These interactions not only affect the photophysical properties of the probes but also create a large uncertainty in their orientation...
  4. pmc Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrier
    Omid Veiseh
    Departments of Materials Science and Engineering, University of Washington, Seattle, Washington 99195, USA
    Cancer Res 69:6200-7. 2009
    ....
  5. ncbi Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci
    Mandana Veiseh
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Cancer Res 67:6882-8. 2007
    ..Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies...
  6. ncbi Evaluation of a proposed in vitro test strategy using neuronal and non-neuronal cell systems for detecting neurotoxicity
    J Gartlon
    ECVAM, European Commission Joint Research Centre, via E Fermi 1, Ispra 21020, Italy
    Toxicol In Vitro 20:1569-81. 2006
    ....
  7. ncbi Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells
    James M Flynn
    Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Mol Vis 12:271-82. 2006
    ....
  8. pmc Photoswitching mechanism of cyanine dyes
    Graham T Dempsey
    Graduate Program in Biophysics, School of Engineering and Applied Sciences, Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Am Chem Soc 131:18192-3. 2009
    ..Mass spectrometry suggests that the photoconversion product is a thiol-cyanine adduct in which covalent attachment of the thiol to the polymethine bridge disrupts the original conjugated pi-electron system of the dye...
  9. ncbi Spontaneous changes in mitochondrial membrane potential in cultured neurons
    J F Buckman
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
    J Neurosci 21:5054-65. 2001
    ..We hypothesize that these DeltaPsi(m) fluctuations are dependent on the proper functioning of the mitochondria and reflect mitochondria alternating between the active and inactive states of oxidative phosphorylation...
  10. pmc Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging
    Richa Dave
    Tri Institutional Program in Chemical Biology, Weill Cornell Medical College of Cornell University, New York, New York, USA
    Biophys J 96:2371-81. 2009
    ..These efforts may ultimately facilitate tuning organic dye molecule performance according to each specific experimental demand...
  11. ncbi Carbocyanine dyes as efficient reversible single-molecule optical switch
    Mike Heilemann
    Applied Laserphysics and Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 127:3801-6. 2005
    ....
  12. ncbi High frequency vibrational modulations in two-dimensional electronic spectra and their resemblance to electronic coherence signatures
    N Christensson
    Electronic Properties of Materials, Faculty of Physics, University of Vienna, Strudlhofgasse 4, 1090 Vienna, Austria
    J Phys Chem B 115:5383-91. 2011
    ..Our results show that these anticorrelated oscillations occur for vibrational wavepackets and not exclusively for electronic coherences as has been assumed previously...
  13. pmc Short-range spectroscopic ruler based on a single-molecule optical switch
    Mark Bates
    Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA
    Phys Rev Lett 94:108101. 2005
    ..This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level...
  14. ncbi Multi-colour direct STORM with red emitting carbocyanines
    André Lampe
    Institute of Chemistry, Freie Universitat Berlin, 14195 Berlin, Germany
    Biol Cell 104:229-37. 2012
    ....
  15. pmc Orientation of cyanine fluorophores terminally attached to DNA via long, flexible tethers
    Jonathan Ouellet
    Cancer Research UK Nucleic Acid Structure Research Group, The University of Dundee, Dundee, United Kingdom
    Biophys J 101:1148-54. 2011
    ..This is consistent with the transition moments becoming approximately aligned with the long axis of the terminal basepair for the long-linker species...
  16. ncbi Accurate distance determination of nucleic acids via Förster resonance energy transfer: implications of dye linker length and rigidity
    Simon Sindbert
    Institut für Physikalische Chemie, Lehrstuhl für Molekulare Physikalische Chemie, Heinrich Heine Universität, Düsseldorf, Germany
    J Am Chem Soc 133:2463-80. 2011
    ..A detailed analysis of possible orientation effects (κ(2) problem) indicates that, for short linkers and unknown local environments, additional κ(2)-related uncertainties are clearly outweighed by better defined dye positions...
  17. ncbi Translation factors direct intrinsic ribosome dynamics during translation termination and ribosome recycling
    Samuel H Sternberg
    Department of Chemistry, Columbia University, New York, New York, USA
    Nat Struct Mol Biol 16:861-8. 2009
    ..Our results support the view that specific regulation of the global state of the ribosome is a fundamental characteristic of all translation factors and a unifying theme throughout protein synthesis...
  18. pmc Lack of neurotrophin 3 causes losses of both classes of spiral ganglion neurons in the cochlea in a region-specific fashion
    B Fritzsch
    Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68178, USA
    J Neurosci 17:6213-25. 1997
    ..Despite these impressive innervation deficiencies, the morphology of the organ of Corti and the development of inner and outer hair cells appeared comparatively normal...
  19. ncbi Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) by using fluorescence-activated flow cytometry
    H David Guthrie
    US Department of Agriculture, Animal Bioscience and Biotechnology Laboratory, Agricultural Research Service, Beltsville, Maryland, USA
    Methods Mol Biol 477:89-97. 2008
    ..We found that the reactive oxygen species generator, menadione reduced sperm motility and reduced Deltapsi(m) in a dose responsive fashion that was closely correlated with the loss of motility...
  20. ncbi Cyanine dyes attenuate cerebral ischemia and reperfusion injury in rats
    Satomi Koya-Miyata
    Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc
    Biol Pharm Bull 33:1872-7. 2010
    ..These results suggest that NK-4 and NK-150 effectively prevented ischemia-induced brain injury through their potent neurotrophin-like activity as well as antioxidative activity...
  21. pmc Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for the study of enzyme activity
    Wonbae Lee
    Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803 1804, USA
    Anal Chem 82:1401-10. 2010
    ..In all cases, no spectral leakage from single-molecule events into inappropriate color channels was observed...
  22. ncbi Maximising sensitivity for detecting changes in protein expression: experimental design using minimal CyDyes
    Natasha A Karp
    Department of Biochemistry, Cambridge University, Downing Site, Cambridge CB2 1QW, UK
    Proteomics 5:3105-15. 2005
    ..Technical variance was shown to encompass both experimental and analytical noise and thus is dependent on the analytical software utilised. Data is provided as a resource to the community to assess alternative software and upgrades...
  23. ncbi DNA sequence-dependent enhancement of Cy3 fluorescence
    Billie Jo Harvey
    Biodesign Institute, Arizona State University, Arizona State University, Tempe, AZ, USA
    Photochem Photobiol Sci 8:1105-10. 2009
    ..We have shown that the ability of Cy3 to interact with DNA depends on the flexibility of the oligonucleotide and the presence of purines in the chain...
  24. ncbi Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
    Judith E Berlier
    Molecular Probes, Inc, Eugene, Oregon 97402, USA
    J Histochem Cytochem 51:1699-712. 2003
    ....
  25. ncbi Fluorescent probes for super-resolution imaging in living cells
    Marta Fernandez-Suarez
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Rev Mol Cell Biol 9:929-43. 2008
    ..We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development...
  26. ncbi Excitonic couplings and interband energy transfer in a double-wall molecular aggregate imaged by coherent two-dimensional electronic spectroscopy
    F Milota
    Department of Physical Chemistry, University of Vienna, Wahringerstrasse 42, A 1090 Vienna, Austria
    J Chem Phys 131:054510. 2009
    ....
  27. pmc Single-molecule FRET and linear dichroism studies of DNA breathing and helicase binding at replication fork junctions
    Carey Phelps
    Oregon Center for Optics, Department of Chemistry and Biochemistry, and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403
    Proc Natl Acad Sci U S A 110:17320-5. 2013
    ....
  28. ncbi Development of improved cell lysis, solubilization and imaging approaches for proteomic analyses
    Richard M Leimgruber
    Pharmacia, Discovery Research and Development, St Louis, MO 63198, USA
    Proteomics 2:135-44. 2002
    ..Using these approaches, it is possible to perform more rapid and accurate comparative analyses and proteomic, post-gel characterization of proteins of interest than using comparative image analysis of multiple gels...
  29. pmc Two-photon optical properties of near-infrared dyes at 1.55 μm excitation
    Mikhail Y Berezin
    Department of Radiology, Washington University, St Louis, Missouri 63110, USA
    J Phys Chem B 115:11530-5. 2011
    ..Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region...
  30. ncbi Localized biosensing with Topas microstructured polymer optical fiber
    Grigoriy Emiliyanov
    COM DTU, Department of Communications, Optics and Materials, Technical University of Denmark, Muilding 345v, 2800 Kgs Lyngby, Denmark
    Opt Lett 32:460-2. 2007
    ..The concept is demonstrated using a fluorescence-based method for selective detection of fluorophore-labeled antibodies...
  31. pmc Lenticular mitoprotection. Part A: Monitoring mitochondrial depolarization with JC-1 and artifactual fluorescence by the glycogen synthase kinase-3β inhibitor, SB216763
    Morgan M Brooks
    Department of Cell Biology and Anatomy, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA
    Mol Vis 19:1406-12. 2013
    ..We demonstrate the novel use of spectral deconvolution to negate the interfering contributing fluorescence by SB216763, thus allowing an unfettered analysis of the JC-1 dye to determine the mitochondrial membrane potential...
  32. pmc Robust and efficient synthetic method for forming DNA microarrays
    P L Dolan
    Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
    Nucleic Acids Res 29:E107-7. 2001
    ..Thus, the ATMS/diazotization chemistry facilitated covalent binding of unmodified DNA, and the reusable microarrays that were produced showed enhanced levels of hybridization and very low background fluorescence...
  33. ncbi Heptamethine cyanine dyes with a robust C-C bond at the central position of the chromophore
    Hyeran Lee
    Department of Radiology, Washington University, St Louis, Missouri 63110, USA
    J Org Chem 71:7862-5. 2006
    ..The results demonstrate the potential of using the new compounds as fluorescent antennae for molecular imaging, spectroscopy, microscopy, and chemical or biological molecular recognition studies...
  34. ncbi Density functional theory and Raman spectroscopy applied to structure and vibrational mode analysis of 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloro- benzimidazolocarbocyanine iodide and its aggregate
    Metin Aydin
    Department of Chemistry, Faculty of Art and Sciences, Ondokuz Mayis University, 55139 Samsun, Turkey
    J Chem Phys 134:064325. 2011
    ....
  35. pmc Optical imaging predicts tumor response to anti-EGFR therapy
    Emily E Helman
    Department of Surgery, Division of Otolaryngology Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, AL, USA
    Cancer Biol Ther 10:166-71. 2010
    ..Tumors from cell lines that showed low internalized signal in vitro responded best to treatment with cetuximab. This preclinical model may aid in determining which cancer patients are best suited for cetuximab therapy...
  36. pmc Neurotrophic effects of a cyanine dye via the PI3K-Akt pathway: attenuation of motor discoordination and neurodegeneration in an ataxic animal model
    Hitomi Ohta
    Research Center, Biomedical Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
    PLoS ONE 6:e17137. 2011
    ..In the screening of biologically active molecules for neurotrophic potency, we found that a photosensitizing cyanine dye, NK-4, had remarkable neurotrophic activities and was a potent radical scavenger...
  37. ncbi Complexation of polymethine dyes with human serum albumin: a spectroscopic study
    Alexander S Tatikolov
    Centro de Quimica Estrutural, Complexo 1, Instituto Superior Tecnico, Av Rovisco Pais, Lisboa 1049 001, Portugal
    Biophys Chem 107:33-49. 2004
    ..Binding to HSA gives rise to dye CD signals and in many cases is accompanied by aggregation of the dyes. These aggregates often exhibit biphasic CD spectra. The aggregates formed by the dyes alone are decomposed in the presence of HSA...
  38. ncbi Synthesis, characterization, and biological properties of cyanine-labeled somatostatin analogues as receptor-targeted fluorescent probes
    K Licha
    Institut für Diagnostikforschung GmbH an der Freien Universität Berlin, 14050 Berlin, Germany
    Bioconjug Chem 12:44-50. 2001
    ..In conclusion, receptor-specific peptides are promising tools for designing site-directed optical contrast agents for use in molecular optical imaging...
  39. pmc Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids
    Asif Iqbal
    Cancer Research United Kingdom Nucleic Acid Structure Research Group, MSI WTB complex, The University of Dundee, Dundee DD1 5EH, United Kingdom
    Proc Natl Acad Sci U S A 105:11176-81. 2008
    ..Our data unequivocally establish that Förster transfer obeys the orientation dependence as expected for a dipole-dipole interaction...
  40. ncbi Ribonuclease protection assay on microchip electrophoresis
    Yuka Yamaguchi
    Health Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 2217 14 Hayashi Cho, Takamatsu, 761 0395, Japan
    Analyst 136:2247-51. 2011
    ..These results clearly indicate the potential of microchip electrophoresis for the analysis of mRNA expression in cells...
  41. ncbi Solid-phase methods for the synthesis of cyanine dyes
    Stephen J Mason
    University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U K
    J Org Chem 70:2939-49. 2005
    ..Overall, both approaches appear to be robust and versatile strategies to delivering a wide range of cyanine-based dyes in high purity...
  42. ncbi Heptamethine cyanine dyes with a large stokes shift and strong fluorescence: a paradigm for excited-state intramolecular charge transfer
    Xiaojun Peng
    State Key Laboratory of Fine Chemicals, Dalian University of Technology, 158 Zhongshan Rd, Dalian 116012, PR China
    J Am Chem Soc 127:4170-1. 2005
    ..They were suggested to be a new paradigm for excited-state intramolecular charge transfer (ICT). The configuration change of the bridgehead amine accompanying ICT was investigated in different viscosity and pH media...
  43. pmc Three color cDNA microarrays: quantitative assessment through the use of fluorescein-labeled probes
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Nucleic Acids Res 31:e14. 2003
    ..80, P < 0.001) was observed. We therefore conclude that slide selection based upon prehybridization quality scores will greatly benefit the ability to generate reliable gene expression data...
  44. pmc Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism
    Qianfan Yang
    Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People s Republic of China
    Nucleic Acids Res 38:1022-33. 2010
    ....
  45. ncbi Nucleobase-specific enhancement of Cy3 fluorescence
    Billie J Harvey
    Biodesign Institute, Arizona State University, Tempe, AZ 85287 5601, USA
    J Fluoresc 19:443-8. 2009
    ..These results are consistent with a model in which Cy3-nucleoside pi-pi interactions decrease the efficiency of photoisomerization,increasing the efficiency of fluorescence...
  46. pmc Engineered knottin peptides: a new class of agents for imaging integrin expression in living subjects
    Richard H Kimura
    Department of Bioengineering, Cancer Center, Bio X Program, Stanford University, Stanford, California, USA
    Cancer Res 69:2435-42. 2009
    ..Thus, engineered integrin-binding knottin peptides show great potential as clinical diagnostics for a variety of cancers...
  47. pmc Synergistic effects of light-emitting probes and peptides for targeting and monitoring integrin expression
    Samuel Achilefu
    Department of Radiology and Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:7976-81. 2005
    ..clearly demonstrate the synergistic effects of Cypate and GRD peptide for molecular recognition of integrin expression and suggest the potential of using carbocyanines as optical scaffolds for designing biologically active molecules.
  48. pmc In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodies
    Yoshinori Koyama
    Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room 1B40, Bethesda, MD, 20892 1088, USA
    Neoplasia 9:1021-9. 2007
    ..Multiple filter sets increase the signal-to-noise ratio by substantially reducing the background signal, and may allow more optical dyes to be resolved within the narrow limits of the near-infrared spectrum...
  49. ncbi Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
    Kristen E Adams
    Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030, USA
    J Biomed Opt 12:024017. 2007
    ..5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered...
  50. ncbi DNA-templated assembly of helical cyanine dye aggregates: a supramolecular chain polymerization
    Kristen C Hannah
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Acc Chem Res 37:845-53. 2004
    ..This Account describes how polymerization depends on the dye structure and DNA sequence and also summarizes the interesting optical properties exhibited by these chiral, helical materials...
  51. ncbi In vivo diagnosis of epidermal growth factor receptor expression using molecular imaging with a cocktail of optically labeled monoclonal antibodies
    Tristan Barrett
    Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Cancer Res 13:6639-48. 2007
    ..Here, we use a "cocktail" of optically labeled monoclonal antibodies directed against EGFR-1 (HER1) and EGFR-2 (HER2) to distinguish tumors by their cell surface expression profiles...
  52. ncbi Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivo
    David E Sosnovik
    Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Circulation 115:1384-91. 2007
    ....
  53. ncbi In vivo detection of head and neck cancer orthotopic xenografts by immunofluorescence
    Eben L Rosenthal
    Department of Surgery, Division of Otolaryngology Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Laryngoscope 116:1636-41. 2006
    ..To determine whether Cy5.5-labeled antiepidermal growth factor (EGFR) antibody could be used to detect head and neck squamous cell carcinoma (HNSCC) xenografts in vivo...
  54. ncbi Prospective isolation of late development multipotent precursors whose migration is promoted by EGFR
    Francesca Ciccolini
    Department of Neurobiology, Interdisciplinary Center for Neurosciences IZN, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany
    Dev Biol 284:112-25. 2005
    ..In addition, it can be used as a marker to effectively isolate telencephalic multipotent precursors from embryonic and adult tissue...
  55. ncbi Diagnosis of arthritis using near-infrared fluorochrome Cy5.5
    Andreas Hansch
    Institute of Diagnostic and Interventional Radiology, Friedrich Schiller University Jena, Jena, Germany
    Invest Radiol 39:626-32. 2004
    ..This applicability study sought to define whether imaging with fluorochrome Cy5.5 can identify arthritis in murine antigen-induced arthritis (AIA)...
  56. ncbi In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in brain tumor xenografts
    Xiaoyuan Chen
    PET Imaging Science Center, University of Southern California Keck School of Medicine, Los Angeles, California, USA
    Cancer Res 64:8009-14. 2004
    ....
  57. pmc Targeting gelatinases with a near-infrared fluorescent cyclic His-Try-Gly-Phe peptide
    Wei Wang
    Department of Experimental Diagnostic Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Mol Imaging Biol 11:424-33. 2009
    ..The purpose of the study is to synthesize and characterize near-infrared (NIR) fluorescence imaging probes targeted to gelatinases...
  58. ncbi Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin
    H J Gruber
    Institute of Biophysics and Institute of Chemistry, J Kepler University, Altenberger Strasse 69, A 4040 Linz, Austria
    Bioconjug Chem 11:696-704. 2000
    ..5, were similar to Cy5. In conclusion the Cy dye series is divided into two groups: Antibodies with multiple Cy3 or Cy3.5 labels yield bright fluorescence while extensive quenching occurs in antibodies labeled with Cy5 and Cy7...
  59. ncbi Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
    V N Dedov
    Department of Pharmacy, University of Sydney, NSW, 2006, Syndey, Australia
    Micron 32:653-60. 2001
    ....
  60. ncbi Accumulation of liposome with Sialyl Lewis X to inflammation and tumor region: application to in vivo bio-imaging
    Masahiko Hirai
    Kobe R and D Center, Katayama Chemical Industries Co, Ltd, Japan
    Biochem Biophys Res Commun 353:553-8. 2007
    ..Thus, it was proven that this liposome is useful not only as an in vivo bio-imaging reagent but also as a drug delivery system (DDS)...
  61. pmc Identification and removal of contaminating fluorescence from commercial and in-house printed DNA microarrays
    M Juanita Martinez
    Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
    Nucleic Acids Res 31:e18. 2003
    ....
  62. pmc Single-pair fluorescence resonance energy transfer (spFRET) for the high sensitivity analysis of low-abundance proteins using aptamers as molecular recognition elements
    Wonbae Lee
    Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803 1804, USA
    J Fluoresc 20:203-13. 2010
    ..We also demonstrate that the technique can discriminate thrombin molecules from its analogue prothrombin. The analytical sensitivity was >200-fold better than an ensemble measurement...
  63. ncbi 1H NMR and optical spectroscopic investigation of the sequence-dependent dimerization of a symmetrical cyanine dye in the DNA minor groove
    Kristen C Hannah
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
    Biochemistry 44:15924-9. 2005
    ....
  64. pmc Flow cytometric probing of mitochondrial function in equine peripheral blood mononuclear cells
    Dominique Cassart
    Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, B 4000 Liege, Belgium
    BMC Vet Res 3:25. 2007
    ....
  65. ncbi False labelling due to quenching failure of N-hydroxy-succinimide-ester-coupled dyes
    Weiqun Wang
    Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, Medical Faculty, University of Munster, Munster, Germany
    Proteomics 10:1525-9. 2010
    ..It could be shown that only a 100-fold increase in the amount of quencher will silence residual dye to such an extent that no artificial signals are detected...
  66. ncbi Suppressive effects of a cyanine dye against herpes simplex virus (HSV)-1 infection
    Chie Ushio
    Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
    Biomed Res 30:365-8. 2009
    ..These results suggest that lumin exerts an antiviral action against HSV-1 with the independent pathways of IFN-alpha and also it would become a therapeutically effective drug in clinical practice...
  67. ncbi Experimental and theoretical study of the interaction of single-stranded DNA homopolymers and a monomethine cyanine dye: nature of specific binding
    Larisa Mikelsons
    Department of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    Photochem Photobiol Sci 4:798-802. 2005
    ....
  68. ncbi Gene-specific dye bias in microarray reference designs
    Alan A Dombkowski
    Institute of Environmental Health Sciences, Wayne State University, 2727 Second Ave, Detroit, MI 48201, USA
    FEBS Lett 560:120-4. 2004
    ..We demonstrate that this assumption is not reliable and that dye orientation can significantly influence measured changes in gene expression...
  69. ncbi In vivo imaging of experimental arthritis with near-infrared fluorescence
    Andreas Hansch
    Institute of Diagnostic and Interventional Radiology, Fredrich Schiller University Jena, Jena, Germany
    Arthritis Rheum 50:961-7. 2004
    ..To visualize early experimental arthritis with near-infrared fluorescence (NIRF) imaging in a murine model of antigen-induced arthritis (AIA)...
  70. ncbi Kinetic mechanism of direct transfer of Escherichia coli SSB tetramers between single-stranded DNA molecules
    Alexander G Kozlov
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Biochemistry 41:11611-27. 2002
    ..Such a mechanism may be used to recycle SSB tetramers between old and newly formed ssDNA regions during lagging strand DNA replication...
  71. ncbi A new method for in vitro detection of microbially produced mitochondrial toxins
    D Hoornstra
    University of Helsinki, Department of Applied Chemistry and Microbiology, Division of Microbiology, PO Box 56, FIN 00014 University of Helsinki, Finland
    Toxicol In Vitro 17:745-51. 2003
    ..Surfactin and lichenysin A caused mitochondrial damage at concentrations where the plasma membrane was also damaged...
  72. ncbi Vessel painting of the microcirculation using fluorescent lipophilic tracers
    Dino J Ravnic
    Laboratory of Immunophysiology, Department of Surgery, Brigham and Women s Hospital, Harvard Surgical Research Laboratories, Harvard Medical School, Room 259, 75 Francis Street, Boston, MA 02115, USA
    Microvasc Res 70:90-6. 2005
    ..These studies suggest the utility of fluorescent vessel painting as a complementary technique to corrosion casting in the morphometric study of the microcirculation...
  73. pmc Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI
    Yiwen Li
    Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, 506 McKnight Building, 1638 NW 10th Avenue, Miami, Florida 33136, USA
    Nat Protoc 3:1703-8. 2008
    ....
  74. ncbi Nobiletin, a citrus flavonoid, suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cells
    Ai Eguchi
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    FEBS Lett 580:3321-8. 2006
    ..Together, our results suggest that nobiletin is a promising phytochemical for regulating atherosclerosis with reasonable action mechanisms...
  75. ncbi Fluorescence properties and photophysics of the sulfoindocyanine Cy3 linked covalently to DNA
    Matthew E Sanborn
    Department of Chemistry and Biochemistry, Department of Physics and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287 5601, USA
    J Phys Chem B 111:11064-74. 2007
    ..The remarkable variations in the photophysical properties of Cy3-DNA constructs demonstrate that caution should be used when Cy3 is used in studies employing DNA conjugates...
  76. pmc Model-based analysis of two-color arrays (MA2C)
    Jun S Song
    Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Genome Biol 8:R178. 2007
    ..The tools have been implemented as a stand-alone Java program called MA2C, which can display various plots of statistical analysis for quality control...
  77. ncbi Intrinsic connectivity of human superior colliculus
    Eric Tardif
    Division de Neuropsychologie, CHUV, 1011, Lausanne, Switzerland
    Exp Brain Res 166:316-24. 2005
    ..The putative roles of these connections are discussed with regard to visual receptive field organization, as well as visuomotor and multisensory integration...
  78. ncbi Near-infrared fluorescent nanoparticles as combined MR/optical imaging probes
    Lee Josephson
    Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown 02129, USA
    Bioconjug Chem 13:554-60. 2002
    ..The probes may provide the basis for a new class of so-called smart nanoparticles, capable of pinpointing their position through their magnetic properties, while providing information on their environment by optical imaging techniques...
  79. ncbi Role reversal in a supramolecular assembly: a chiral cyanine dye controls the helicity of a peptide-nucleic acid duplex
    Babu Rao Renikuntla
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
    Langmuir 21:5362-6. 2005
    ..These results indicate that chirality is transferred from the dye to the PNA, as opposed to other examples of polymer-templated dye aggregation where chirality is transferred from the template to the dye...
  80. ncbi G-quadruplex DNA binding by a series of carbocyanine dyes
    S M Kerwin
    Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, 78712, USA
    Bioorg Med Chem Lett 11:2411-4. 2001
    ..Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration...
  81. ncbi Effects of a modified dye-labeled nucleotide spacer arm on incorporation by thermophilic DNA polymerases
    Christopher Lacenere
    Division of Biology, California Institute of Technology, Pasadena, California, USA
    Nucleosides Nucleotides Nucleic Acids 25:9-15. 2006
    ..Furthermore, some of the assayed polymerases are capable of extending the primer to the full or near full length of 30 nucleotides using dye-labeled nucleotides exclusively...
  82. ncbi Chiral transformation of achiral J-aggregates of a cyanine dye templated by human serum albumin
    Yazhou Zhang
    Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P R China
    Chemphyschem 8:224-6. 2007
  83. ncbi In vivo imaging of integrin alpha v beta 3 expression using fluorescence-mediated tomography
    Angelika von Wallbrunn
    Department of Clinical Radiology, University of Munster, Albert Schweitzer Strasse 33, 48129, Munster, Germany
    Eur J Nucl Med Mol Imaging 34:745-54. 2007
    ..We therefore synthesised an alpha v beta(3) targeted fluorochrome and imaged tumour xenografts with different alpha v beta(3) expression levels using both planar and tomographic optical imaging methods...
  84. ncbi Nonblinking and long-lasting single-molecule fluorescence imaging
    Ivan Rasnik
    Department of Physics, Emory University, Atlanta, Georgia 30322, USA
    Nat Methods 3:891-3. 2006
    ....
  85. ncbi The combined effects of trkB and trkC mutations on the innervation of the inner ear
    B Fritzsch
    Creighton University, Department of Biomedical Sciences, Omaha, NE 68178, USA
    Int J Dev Neurosci 16:493-505. 1998
    ....
  86. ncbi Location of cyanine-3 on double-stranded DNA: importance for fluorescence resonance energy transfer studies
    D G Norman
    Department of Biochemistry, The University of Dundee, UK
    Biochemistry 39:6317-24. 2000
    ..The knowledge of the fluorophore positions can now be used for more precise interpretation of FRET data from nucleic acids...
  87. pmc Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements
    William S Branham
    Center for Functional Genomics, Division of Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079, USA
    BMC Biotechnol 7:8. 2007
    ..Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy5 signal relative to the Cy3 signal in 2-color microarrays will adversely reduce the Cy5/Cy3 ratio resulting in unreliable microarray data...
  88. ncbi Multiplex detection and quantitation of proteins on western blots using fluorescent probes
    J C Gingrich
    Bio Rad Laboratories, Hercules, CA, USA
    Biotechniques 29:636-42. 2000
    ..Two examples of three-color multiplex detection using FITC-, Cy3- and Cy5-conjugated probes on Western blots are provided to demonstrate applications of this approach...
  89. pmc Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes
    J B Randolph
    Center for Light Microscope Imaging and Biotechnology and Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Nucleic Acids Res 25:2923-9. 1997
    ..A mechanism that accounts for this destabilization is proposed which is consistent with our data. It involves dye-dye and dye-nucleotide interactions which appear to stabilize a single-stranded conformation of the probe...
  90. ncbi Investigation of DNA binding modes for a symmetrical cyanine dye trication: effect of DNA sequence and structure
    R Cao
    Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213-3890, USA
    J Biomol Struct Dyn 18:844-56. 2001
    ..Seifert, J.L., et al. (1999) J. Am. Chem. Soc. 121, 2987-2995] These studies illustrate the diversity of DNA binding modes that are available to a given ligand structure...
  91. pmc Optimization of oligonucleotide-based DNA microarrays
    Angela Relógio
    Gene Expression Programme and Functional Genomics Technology and Instrumentation Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nucleic Acids Res 30:e51. 2002
    ..These data will facilitate the design and standardization of custom-made microarrays applicable to gene expression profiling and sequencing analyses...
  92. ncbi A multimodal nanoparticle for preoperative magnetic resonance imaging and intraoperative optical brain tumor delineation
    Moritz F Kircher
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Cancer Res 63:8122-5. 2003
    ..This prototypical multimodal nanoparticle has unique properties that may allow radiologists and neurosurgeons to see the same probe in the same cells and may offer a new approach for obtaining tumor margins...
  93. pmc Triple-target microarray experiments: a novel experimental strategy
    Thorsten Forster
    Scottish Centre for Genomic Technology and Informatics, University of Edinburgh, The Chancellor s Building, College of Medicine, 49 Little France Crescent, Edinburgh, EH16 4SB, UK
    BMC Genomics 5:13. 2004
    ....
  94. ncbi Live imaging of mammalian retina: rod outer segments are stained by conventional mitochondrial dyes
    Paolo Bianchini
    University of Genoa, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy LAMBS, MicroSCoBiO Research Center, Department of Physics, Via Dodecaneso 33, 16146, Genoa, Italy
    J Biomed Opt 13:054017. 2008
    ..This finding may shed light on previous reports of the existence of a proton potential across the disk membranes and on the mechanism of the adenosine tri-phosphate (ATP) supply for phototransduction, which still requires investigation...
  95. ncbi Olivary projection from the rostral part of the nucleus of Darkschewitsch in the postnatal rat as revealed through the use of a carbocyanine dye
    Satoru Onodera
    Department of Anatomy, School of Medicine, Iwate Medical University, Uchimaru 19 1, Morioka 020 8505, Japan
    Brain Res 1015:194-7. 2004
    ..The present study suggests the existence of a precise topographic organization from subnuclei of the area parafascicularlis prerublaris to subnuclei of the inferior olive...
  96. pmc Intensity-based analysis of two-colour microarrays enables efficient and flexible hybridization designs
    Peter A C 't Hoen
    Center for Human and Clinical Genetics, Leiden University Medical Center, The Netherlands
    Nucleic Acids Res 32:e41. 2004
    ....
  97. pmc An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments
    Colin Echeverría Aitken
    Biophysics Program, Stanford University School of Medicine, Stanford, California 94305 5126, USA
    Biophys J 94:1826-35. 2008
    ..We observed increased stability of Cy3 and Alexa488 in the presence of the antioxidants ascorbic acid and n-propyl gallate. This new O(2)-scavenging system should have wide application for single-molecule fluorescence experiments...
  98. pmc Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particles
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
    Anal Biochem 317:136-46. 2003
    ..These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays...
  99. ncbi Effects of atmospheric ozone on microarray data quality
    Thomas L Fare
    Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA
    Anal Chem 75:4672-5. 2003
    ..To address this environmental effect, laboratory ozone levels should be kept below 2 ppb (e.g., with filters in HVAC) to achieve high quality microarray data...
  100. pmc Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
    Joanna Malicka
    Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Anal Biochem 315:57-66. 2003
    ..These results suggest that maximum fluorescence enhancements occur about 90A from the silver surface, a distance readily obtained by one or two layers of proteins...
  101. ncbi Comparison of two tricarbocyanine-based dyes for fluorescence optical imaging
    Christin Perlitz
    Optical Imaging and New Modalities Research, Research Laboratories, Schering AG, Müllerstrasse, 13342 Berlin, Germany
    J Fluoresc 15:443-54. 2005
    ..The primary aim was to modify the physicochemical properties of the previously synthesized dye SIDAG to investigate the effect on the in vivo imaging properties...

Research Grants63

  1. Integrated Instrumentation for Proteomics
    Helen Kim; Fiscal Year: 2002
    ....
  2. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
    ..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
  3. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2005
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  4. NOVEL TYROSINASE MUTANTS AS IMAGING MARKER GENES
    Alexei Bogdanov; Fiscal Year: 2002
    ..The long-term goal of this research is to explore and develop novel and useful bifunctional "imaging/therapeutic marker genes" for in vivo use. ..
  5. Transcription Factor Reporter Technology
    Alexei Bogdanov; Fiscal Year: 2006
    ..abstract_text> ..
  6. CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILURE
    Joel Weinberg; Fiscal Year: 2007
    ..abstract_text> ..
  7. Biohazard Detection Using Metal-Enhanced Fluorescence
    Joseph Lakowicz; Fiscal Year: 2003
    ..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
  8. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2006
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  9. STRUCTURE AND PLASTICITY OF CENTRAL HEARING MECHANISMS
    CRAIG HENKEL; Fiscal Year: 2002
    ..Results should lead to a better understanding of the influence of experience on the development of these binaural sensory pathways. ..
  10. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2006
    ..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
  11. Dissection of cellular interactions with integrated functional genomics in comple
    Martin Hessner; Fiscal Year: 2008
    ..Understanding the relationship between mast cells, T effector, and TREG cells in autoimmunity and has the potential to establish a new paradigm for immune regulation and create new insights to human disease. ..
  12. CENTER FOR FLUORESCENCE SPECTROSCOPY
    Joseph Lakowicz; Fiscal Year: 2006
    ..Abstract Not Provided. ..
  13. HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITS
    CRAIG HENKEL; Fiscal Year: 2005
    ....
  14. A role for dopamine in brain development
    Pradeep Bhide; Fiscal Year: 2007
    ..It is also an excellent model to mimic dopamine imbalance in the CNS that can be produced by therapeutic drugs that intentionally or unintentionally target the dopaminergic system of the developing brain. ..
  15. Membrane protein electrophoresis in lipid bilayers
    Mary Wirth; Fiscal Year: 2006
    ..If feasible, an R01 proposal would address separations of real mixtures of membrane proteins from cell lysates. The work could have enormous impact on drug discovery, allowing inexpensive screening that encompasses many receptors. ..
  16. Non-Radial Cell Migration in CNS Development
    Jeffrey A Golden; Fiscal Year: 2010
    ..abstract_text> ..
  17. SINGLE-MOLECULE ANALYSIS OF A TRANSCRIPTION ENHANCER
    Larry Friedman; Fiscal Year: 2006
    ..The single-molecule approach will highlight relations between the mechanisms at successive steps, thus contributing to an integrated description of the enhancer-mediated initiation process in this model system. ..
  18. Workshop on Molecular Imaging Agents
    David Vera; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  19. Role of the Foxn4 Gene during Retinogenesis
    Mengqing Xiang; Fiscal Year: 2007
    ..abstract_text> ..
  20. Pulmonary Mitochondrial Injury Caused by Tetrahydrocannabinol
    Theodore Sarafian; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. MULTIMODAL DOT-PET MOLECULAR PROBES AND TANDEM SYSTEM
    Joseph P Culver; Fiscal Year: 2010
    ..abstract_text> ..
  22. Effects of Methylmercury on Neuronal Cell Migration
    Hitoshi Komuro; Fiscal Year: 2010
    ..Answers to the questions raised in this project will provide insights for development of therapies that counter MeHg-induced brain malformation. ..
  23. Tissue-Specific Optical Imaging Agents and Methods
    Samuel Achilefu; Fiscal Year: 2007
    ..Products of this study will also be made available to other investigators interested in molecular-probe- mediated optical imaging and related medical/pharmaceutical applications. ..
  24. A new general methodology for studying conformational dynamics in single cells
    Marcia Levitus; Fiscal Year: 2008
    ..Understanding chromatin dynamics is a key step in the process of elucidating the mechanisms of development, differentiation and cancer. [unreadable] [unreadable] [unreadable]..
  25. Optical Probes & Methods for Imaging Integrin Expression
    Samuel Achilefu; Fiscal Year: 2008
    ..All these products will be made available to other investigators. ..
  26. AAV Vector Targeting Strategies for Gene Therapy
    Jeffrey Bartlett; Fiscal Year: 2008
    ..abstract_text> ..
  27. CYTOMETRIC CD4 COUNTING - A MINIMALIST APPROACH
    Howard Shapiro; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  28. Interactions of Lipoproteins with the Vascular Wall
    JOHN CALVERT RUTLEDGE; Fiscal Year: 2010
    ....
  29. ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE AND SURGERY CONFERENCE
    Samuel Achilefu; Fiscal Year: 2007
    ..unreadable] Aim 4: To promote interaction between the invited scientists and participating students, and to enable [unreadable] collaborations. [unreadable] [unreadable] [unreadable]..
  30. Ultrasound Monitoring of Rat Metastatic Uveal Melanoma
    RODNEY BRAUN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  31. INIA: Neurohistology
    ANDREA ELBERGER; Fiscal Year: 2006
    ..The final product produced by the Core will be tissue sections on microscope slides with appropriate coding to track each case. A database will be maintained in the Core to assure accuracy of data analyses. ..
  32. CELL MIGRATION AND CNS DEVELOPMENT ANOMALIES
    Jeffrey Golden; Fiscal Year: 2002
    ..abstract_text> ..
  33. BLOOD POOL CONTRAST AGENTS FOR CT AND MRI
    David Vera; Fiscal Year: 2002
    ..Additionally, this structure will serve as a neural carrier of for future class of nonparticulate receptor-binding CT and MR contrast media. ..
  34. DEVELOPMENT OF THE OLFACTORY SYSTEM IN ZEBRAFISH
    KATHLEEN WHITLOCK; Fiscal Year: 2004
    ..abstract_text> ..
  35. CONFORMATIONALLY ASSISTED FET PROBES
    Ronald Cook; Fiscal Year: 2002
    ..It is estimated that probe cost will diminish from the current average of $400 per probe to less than $100 per probe, thus opening quenched probes to entirely new applications and markets. ..
  36. DESIGN, SYNTHESIS AND STUDY OF NEW SENSITIZERS FOR PDT
    MICHAEL DETTY; Fiscal Year: 2004
    ....
  37. HIGH-PRECISION FLOW CYTOMETRY OF BACTERIA AND VIRUSES
    Howard Shapiro; Fiscal Year: 2004
    ..The relationship between staining intensity and nucleic acid type and content in viruses, will be investigated, with a view toward refining cytometric virus detection and identification. ..
  38. MicroPET and NIR Fluorescence Imaging Tumor Angiogenesis
    Xiaoyuan Chen; Fiscal Year: 2004
    ..All the results obtained here will be used for future application of a R01 type grant. ..
  39. Excitotoxic Signaling in HD Transgenic Neurons
    KARI HOYT; Fiscal Year: 2004
    ..The cell culture models of HD proposed in this work should also provide a convenient means for testing relevant therapies for HD. ..
  40. High Performance Lanthanide DNA Probes
    Ronald Cook; Fiscal Year: 2002
    ..As a result the practical and economic synthesis of lanthanide oligo probes will become possible, and their application to genomic assays can begin. ..
  41. Effects of alcohol on neuronal cell migration
    Hitoshi Komuro; Fiscal Year: 2005
    ..Answers to the questions raised in this project will provide a new insight for understanding how prenatal and early postnatal exposure to alcohol causes malformation of brain. ..
  42. Roles of Homeodomain Factors in Neural Development
    Mengqing Xiang; Fiscal Year: 2004
    ....
  43. Hybrid optical-ultrasound scanner for cancer imaging
    Vasilis Ntziachristos; Fiscal Year: 2005
    ..abstract_text> ..
  44. Mechanisms of Auto-Rhythmicity in Heart Development
    MARGARET KIRBY; Fiscal Year: 2005
    ..The results from the completion of the proposed experiments will lead to a greater understanding of cardiac arhythmias in human heart disease and facilitate the design of treatment strategies. ..
  45. A Novel Immunotoxin for Treatment and Cure of Strabismus
    LEO CHALUPA; Fiscal Year: 2005
    ..The use of IX-146 could be extended in the future to a wider variety of ophthalmologic, neurologic, and neuromuscular disorders, including blepharospasm as well as other types of dystonias. ..
  46. NMR AND FUNCTIONAL ANALYSIS OF ONCOGENE PRODUCTS
    Markus Germann; Fiscal Year: 2001
    ..The solution structure is of particular relevance for the design of agents that act as inhibitors of oncogene function. Inhibitors of hTCL-1 and hMTCP-1 have potential for treatment of low-grade T-cell lymphomas and leukemias. ..
  47. ROLES OF NITRIC OXIDE AND OXYGEN IN OCULAR MELANOMA
    RODNEY BRAUN; Fiscal Year: 2002
    ....
  48. NEUROPHYSIOLOGY OF CRANIAL HEADACHE
    Andrew Strassman; Fiscal Year: 2008
    ..This information can then be used to develop strategies for blocking the neural changes that result in headache. [unreadable] [unreadable]..
  49. Imaging of gene expression in glioblastoma
    JAMES PETER BASILION; Fiscal Year: 2010
    ..The conceptual scheme presented herein is also potentially applicable to a variety of diseases for which gene profiling analyses is available. ..
  50. Symposium on Imaging Agents and Molecular Beacons
    Samuel Achilefu; Fiscal Year: 2004
    ..Five sessions will be held and will cover topics on nuclear, magnetic resonance, ultrasound, radiographic, optical and miscellaneous imaging agents for medical applications. ..
  51. DNA-based Arrays of Cross-reactive Molecular Sensors
    Milan Stojanovic; Fiscal Year: 2004
    ..In the next phase, DNA-based cross-reactive sensors for oligosaccharides will be incorporated into arrays to expand the panel of metabolites that could be profiled in a single-step assay. ..
  52. NMR STRUCTURE OF ZINC FINGERS SPECIFIC FOR HIV RRE RNA
    Markus Germann; Fiscal Year: 2005
    ..The inhibitory activity of designed zinc fingers will be determined through in vitro and in vivo assays. ..
  53. DEVELOPMENT OF THE GUSTATORY SYSTEM
    RICHARD NORTHCUTT; Fiscal Year: 2005
    ..To test the hypothesis that retinoic acid acts as a signal to pattern endoderm directly during gastrulation (aim 3), presumptive pharyngeal endoderm will be excised, exposed to retinoic acid, and cultured. ..
  54. High Performance Lanthanide DNA Probes
    Ronald Cook; Fiscal Year: 2005
    ..We will seek to take advantage of the long fluorescence lifetimes of lanthanide reporters by employing "gated" detection of lanthanide fluorescence under thermocycling conditions for PCR assays. ..
  55. Protein ubiquitination based neuroprotection for stroke
    Robert Meller; Fiscal Year: 2006
    ....
  56. Genetic remodeling of Vagal Afferent Organization
    Edward Fox; Fiscal Year: 2009
    ..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
  57. Layer-by-layer assembly for making drug-eluting stents
    Kinam Park; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  58. Rapid ischemic tolerance: Ubiquitin-mediated structural reorganization
    Robert Meller; Fiscal Year: 2007
    ..Indeed, the long-term aim of these studies is to discover endogenous protective mechanisms that can be translated into effective rapid acting neuroprotective agents for stroke. [unreadable] [unreadable] [unreadable]..
  59. Microenvironment-Controlled Encapsulation (MICE) Process
    Kinam Park; Fiscal Year: 2005
    ..The MICE process will facilitate microencapsulation of a variety of protein drugs that have been difficult to formulate with the conventional methods, as well as small molecular weight drugs. ..
  60. Solvent exchange methods for protein microencapsulation
    Kinam Park; Fiscal Year: 2006
    ..Because of the simplicity of the solvent exchange method, it is expected to open a new avenue of microencapsulation of protein drugs with various properties. ..
  61. Hydrotropic Polymers for oral delivery of paclitaxel
    Kinam Park; Fiscal Year: 2008
    ..The oral paclitaxel formulations are expected to have great therapeutic potential not only for cancer treatment as well as neurodegenerative diseases that can be potentially treated by paclitaxel. ..
  62. RADIOIMMUNOTHERAPY OF B CELL LYMPHOMA WITH ANTI CD74
    M Mattes; Fiscal Year: 2002
    ..This study may lead to the development of more effective radio-conjugates for the therapy of cancer and other diseases. Also, it will provide basic information about the toxic effects of intracellular (really lysosomal) radionuclides. ..