Genomes and Genes
Summary: Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
Publications246 found, 100 shown here
- A G-quadruplex ligand 3,3'-diethyloxadicarbocyanine iodide induces mitochondrion-mediated apoptosis but not decrease of telomerase activity in nasopharyngeal carcinoma NPC-TW01 cellsChung Pin Li
Cooperative Laboratory, Cancer Research Division, National Health Research Institutes, Taipei, Taiwan, ROC
Pharm Res 21:93-100. 2004..In this study, a mitochondrion-mediated apoptotic pathway was demonstrated as another cytotoxic mechanism for DODC action...
- Multicolor super-resolution imaging with photo-switchable fluorescent probesMark Bates
School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
Science 317:1749-53. 2007..Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale...
- Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environmentsMarcia Levitus
Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, Arizona, USA
Q Rev Biophys 44:123-51. 2011..These interactions not only affect the photophysical properties of the probes but also create a large uncertainty in their orientation...
- Specific targeting of brain tumors with an optical/magnetic resonance imaging nanoprobe across the blood-brain barrierOmid Veiseh
Departments of Materials Science and Engineering, University of Washington, Seattle, Washington 99195, USA
Cancer Res 69:6200-7. 2009....
- Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer fociMandana Veiseh
Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N, Seattle, WA 98109, USA
Cancer Res 67:6882-8. 2007..Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies...
- Evaluation of a proposed in vitro test strategy using neuronal and non-neuronal cell systems for detecting neurotoxicityJ Gartlon
ECVAM, European Commission Joint Research Centre, via E Fermi 1, Ispra 21020, Italy
Toxicol In Vitro 20:1569-81. 2006....
- Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cellsJames M Flynn
Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
Mol Vis 12:271-82. 2006....
- Photoswitching mechanism of cyanine dyesGraham T Dempsey
Graduate Program in Biophysics, School of Engineering and Applied Sciences, Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
J Am Chem Soc 131:18192-3. 2009..Mass spectrometry suggests that the photoconversion product is a thiol-cyanine adduct in which covalent attachment of the thiol to the polymethine bridge disrupts the original conjugated pi-electron system of the dye...
- Spontaneous changes in mitochondrial membrane potential in cultured neuronsJ F Buckman
Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
J Neurosci 21:5054-65. 2001..We hypothesize that these DeltaPsi(m) fluctuations are dependent on the proper functioning of the mitochondria and reflect mitochondria alternating between the active and inactive states of oxidative phosphorylation...
- Mitigating unwanted photophysical processes for improved single-molecule fluorescence imagingRicha Dave
Tri Institutional Program in Chemical Biology, Weill Cornell Medical College of Cornell University, New York, New York, USA
Biophys J 96:2371-81. 2009..These efforts may ultimately facilitate tuning organic dye molecule performance according to each specific experimental demand...
- Carbocyanine dyes as efficient reversible single-molecule optical switchMike Heilemann
Applied Laserphysics and Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
J Am Chem Soc 127:3801-6. 2005....
- High frequency vibrational modulations in two-dimensional electronic spectra and their resemblance to electronic coherence signaturesN Christensson
Electronic Properties of Materials, Faculty of Physics, University of Vienna, Strudlhofgasse 4, 1090 Vienna, Austria
J Phys Chem B 115:5383-91. 2011..Our results show that these anticorrelated oscillations occur for vibrational wavepackets and not exclusively for electronic coherences as has been assumed previously...
- Short-range spectroscopic ruler based on a single-molecule optical switchMark Bates
Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA
Phys Rev Lett 94:108101. 2005..This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level...
- Multi-colour direct STORM with red emitting carbocyaninesAndré Lampe
Institute of Chemistry, Freie Universitat Berlin, 14195 Berlin, Germany
Biol Cell 104:229-37. 2012....
- Orientation of cyanine fluorophores terminally attached to DNA via long, flexible tethersJonathan Ouellet
Cancer Research UK Nucleic Acid Structure Research Group, The University of Dundee, Dundee, United Kingdom
Biophys J 101:1148-54. 2011..This is consistent with the transition moments becoming approximately aligned with the long axis of the terminal basepair for the long-linker species...
- Accurate distance determination of nucleic acids via Förster resonance energy transfer: implications of dye linker length and rigiditySimon Sindbert
Institut für Physikalische Chemie, Lehrstuhl für Molekulare Physikalische Chemie, Heinrich Heine Universität, Düsseldorf, Germany
J Am Chem Soc 133:2463-80. 2011..A detailed analysis of possible orientation effects (κ(2) problem) indicates that, for short linkers and unknown local environments, additional κ(2)-related uncertainties are clearly outweighed by better defined dye positions...
- Translation factors direct intrinsic ribosome dynamics during translation termination and ribosome recyclingSamuel H Sternberg
Department of Chemistry, Columbia University, New York, New York, USA
Nat Struct Mol Biol 16:861-8. 2009..Our results support the view that specific regulation of the global state of the ribosome is a fundamental characteristic of all translation factors and a unifying theme throughout protein synthesis...
- Lack of neurotrophin 3 causes losses of both classes of spiral ganglion neurons in the cochlea in a region-specific fashionB Fritzsch
Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68178, USA
J Neurosci 17:6213-25. 1997..Despite these impressive innervation deficiencies, the morphology of the organ of Corti and the development of inner and outer hair cells appeared comparatively normal...
- Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) by using fluorescence-activated flow cytometryH David Guthrie
US Department of Agriculture, Animal Bioscience and Biotechnology Laboratory, Agricultural Research Service, Beltsville, Maryland, USA
Methods Mol Biol 477:89-97. 2008..We found that the reactive oxygen species generator, menadione reduced sperm motility and reduced Deltapsi(m) in a dose responsive fashion that was closely correlated with the loss of motility...
- Cyanine dyes attenuate cerebral ischemia and reperfusion injury in ratsSatomi Koya-Miyata
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc
Biol Pharm Bull 33:1872-7. 2010..These results suggest that NK-4 and NK-150 effectively prevented ischemia-induced brain injury through their potent neurotrophin-like activity as well as antioxidative activity...
- Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for the study of enzyme activityWonbae Lee
Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803 1804, USA
Anal Chem 82:1401-10. 2010..In all cases, no spectral leakage from single-molecule events into inappropriate color channels was observed...
- Maximising sensitivity for detecting changes in protein expression: experimental design using minimal CyDyesNatasha A Karp
Department of Biochemistry, Cambridge University, Downing Site, Cambridge CB2 1QW, UK
Proteomics 5:3105-15. 2005..Technical variance was shown to encompass both experimental and analytical noise and thus is dependent on the analytical software utilised. Data is provided as a resource to the community to assess alternative software and upgrades...
- DNA sequence-dependent enhancement of Cy3 fluorescenceBillie Jo Harvey
Biodesign Institute, Arizona State University, Arizona State University, Tempe, AZ, USA
Photochem Photobiol Sci 8:1105-10. 2009..We have shown that the ability of Cy3 to interact with DNA depends on the flexibility of the oligonucleotide and the presence of purines in the chain...
- Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugatesJudith E Berlier
Molecular Probes, Inc, Eugene, Oregon 97402, USA
J Histochem Cytochem 51:1699-712. 2003....
- Fluorescent probes for super-resolution imaging in living cellsMarta Fernandez-Suarez
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Nat Rev Mol Cell Biol 9:929-43. 2008..We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development...
- Excitonic couplings and interband energy transfer in a double-wall molecular aggregate imaged by coherent two-dimensional electronic spectroscopyF Milota
Department of Physical Chemistry, University of Vienna, Wahringerstrasse 42, A 1090 Vienna, Austria
J Chem Phys 131:054510. 2009....
- Single-molecule FRET and linear dichroism studies of DNA breathing and helicase binding at replication fork junctionsCarey Phelps
Oregon Center for Optics, Department of Chemistry and Biochemistry, and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403
Proc Natl Acad Sci U S A 110:17320-5. 2013....
- Development of improved cell lysis, solubilization and imaging approaches for proteomic analysesRichard M Leimgruber
Pharmacia, Discovery Research and Development, St Louis, MO 63198, USA
Proteomics 2:135-44. 2002..Using these approaches, it is possible to perform more rapid and accurate comparative analyses and proteomic, post-gel characterization of proteins of interest than using comparative image analysis of multiple gels...
- Two-photon optical properties of near-infrared dyes at 1.55 μm excitationMikhail Y Berezin
Department of Radiology, Washington University, St Louis, Missouri 63110, USA
J Phys Chem B 115:11530-5. 2011..Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region...
- Localized biosensing with Topas microstructured polymer optical fiberGrigoriy Emiliyanov
COM DTU, Department of Communications, Optics and Materials, Technical University of Denmark, Muilding 345v, 2800 Kgs Lyngby, Denmark
Opt Lett 32:460-2. 2007..The concept is demonstrated using a fluorescence-based method for selective detection of fluorophore-labeled antibodies...
- Lenticular mitoprotection. Part A: Monitoring mitochondrial depolarization with JC-1 and artifactual fluorescence by the glycogen synthase kinase-3β inhibitor, SB216763Morgan M Brooks
Department of Cell Biology and Anatomy, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA
Mol Vis 19:1406-12. 2013..We demonstrate the novel use of spectral deconvolution to negate the interfering contributing fluorescence by SB216763, thus allowing an unfettered analysis of the JC-1 dye to determine the mitochondrial membrane potential...
- Robust and efficient synthetic method for forming DNA microarraysP L Dolan
Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
Nucleic Acids Res 29:E107-7. 2001..Thus, the ATMS/diazotization chemistry facilitated covalent binding of unmodified DNA, and the reusable microarrays that were produced showed enhanced levels of hybridization and very low background fluorescence...
- Heptamethine cyanine dyes with a robust C-C bond at the central position of the chromophoreHyeran Lee
Department of Radiology, Washington University, St Louis, Missouri 63110, USA
J Org Chem 71:7862-5. 2006..The results demonstrate the potential of using the new compounds as fluorescent antennae for molecular imaging, spectroscopy, microscopy, and chemical or biological molecular recognition studies...
- Density functional theory and Raman spectroscopy applied to structure and vibrational mode analysis of 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloro- benzimidazolocarbocyanine iodide and its aggregateMetin Aydin
Department of Chemistry, Faculty of Art and Sciences, Ondokuz Mayis University, 55139 Samsun, Turkey
J Chem Phys 134:064325. 2011....
- Optical imaging predicts tumor response to anti-EGFR therapyEmily E Helman
Department of Surgery, Division of Otolaryngology Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, AL, USA
Cancer Biol Ther 10:166-71. 2010..Tumors from cell lines that showed low internalized signal in vitro responded best to treatment with cetuximab. This preclinical model may aid in determining which cancer patients are best suited for cetuximab therapy...
- Neurotrophic effects of a cyanine dye via the PI3K-Akt pathway: attenuation of motor discoordination and neurodegeneration in an ataxic animal modelHitomi Ohta
Research Center, Biomedical Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
PLoS ONE 6:e17137. 2011..In the screening of biologically active molecules for neurotrophic potency, we found that a photosensitizing cyanine dye, NK-4, had remarkable neurotrophic activities and was a potent radical scavenger...
- Complexation of polymethine dyes with human serum albumin: a spectroscopic studyAlexander S Tatikolov
Centro de Quimica Estrutural, Complexo 1, Instituto Superior Tecnico, Av Rovisco Pais, Lisboa 1049 001, Portugal
Biophys Chem 107:33-49. 2004..Binding to HSA gives rise to dye CD signals and in many cases is accompanied by aggregation of the dyes. These aggregates often exhibit biphasic CD spectra. The aggregates formed by the dyes alone are decomposed in the presence of HSA...
- Synthesis, characterization, and biological properties of cyanine-labeled somatostatin analogues as receptor-targeted fluorescent probesK Licha
Institut für Diagnostikforschung GmbH an der Freien Universität Berlin, 14050 Berlin, Germany
Bioconjug Chem 12:44-50. 2001..In conclusion, receptor-specific peptides are promising tools for designing site-directed optical contrast agents for use in molecular optical imaging...
- Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acidsAsif Iqbal
Cancer Research United Kingdom Nucleic Acid Structure Research Group, MSI WTB complex, The University of Dundee, Dundee DD1 5EH, United Kingdom
Proc Natl Acad Sci U S A 105:11176-81. 2008..Our data unequivocally establish that Förster transfer obeys the orientation dependence as expected for a dipole-dipole interaction...
- Ribonuclease protection assay on microchip electrophoresisYuka Yamaguchi
Health Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 2217 14 Hayashi Cho, Takamatsu, 761 0395, Japan
Analyst 136:2247-51. 2011..These results clearly indicate the potential of microchip electrophoresis for the analysis of mRNA expression in cells...
- Solid-phase methods for the synthesis of cyanine dyesStephen J Mason
University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U K
J Org Chem 70:2939-49. 2005..Overall, both approaches appear to be robust and versatile strategies to delivering a wide range of cyanine-based dyes in high purity...
- Heptamethine cyanine dyes with a large stokes shift and strong fluorescence: a paradigm for excited-state intramolecular charge transferXiaojun Peng
State Key Laboratory of Fine Chemicals, Dalian University of Technology, 158 Zhongshan Rd, Dalian 116012, PR China
J Am Chem Soc 127:4170-1. 2005..They were suggested to be a new paradigm for excited-state intramolecular charge transfer (ICT). The configuration change of the bridgehead amine accompanying ICT was investigated in different viscosity and pH media...
- Three color cDNA microarrays: quantitative assessment through the use of fluorescein-labeled probesMartin J Hessner
The Max McGee National Research Center for Juvenile Diabetes, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Nucleic Acids Res 31:e14. 2003..80, P < 0.001) was observed. We therefore conclude that slide selection based upon prehybridization quality scores will greatly benefit the ability to generate reliable gene expression data...
- Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanismQianfan Yang
Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People s Republic of China
Nucleic Acids Res 38:1022-33. 2010....
- Nucleobase-specific enhancement of Cy3 fluorescenceBillie J Harvey
Biodesign Institute, Arizona State University, Tempe, AZ 85287 5601, USA
J Fluoresc 19:443-8. 2009..These results are consistent with a model in which Cy3-nucleoside pi-pi interactions decrease the efficiency of photoisomerization,increasing the efficiency of fluorescence...
- Engineered knottin peptides: a new class of agents for imaging integrin expression in living subjectsRichard H Kimura
Department of Bioengineering, Cancer Center, Bio X Program, Stanford University, Stanford, California, USA
Cancer Res 69:2435-42. 2009..Thus, engineered integrin-binding knottin peptides show great potential as clinical diagnostics for a variety of cancers...
- Synergistic effects of light-emitting probes and peptides for targeting and monitoring integrin expressionSamuel Achilefu
Department of Radiology and Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 102:7976-81. 2005..clearly demonstrate the synergistic effects of Cypate and GRD peptide for molecular recognition of integrin expression and suggest the potential of using carbocyanines as optical scaffolds for designing biologically active molecules.
- In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodiesYoshinori Koyama
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room 1B40, Bethesda, MD, 20892 1088, USA
Neoplasia 9:1021-9. 2007..Multiple filter sets increase the signal-to-noise ratio by substantially reducing the background signal, and may allow more optical dyes to be resolved within the narrow limits of the near-infrared spectrum...
- Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancerKristen E Adams
Baylor College of Medicine, Department of Radiology, One Baylor Plaza, MS 360, Houston, Texas 77030, USA
J Biomed Opt 12:024017. 2007..5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered...
- DNA-templated assembly of helical cyanine dye aggregates: a supramolecular chain polymerizationKristen C Hannah
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
Acc Chem Res 37:845-53. 2004..This Account describes how polymerization depends on the dye structure and DNA sequence and also summarizes the interesting optical properties exhibited by these chiral, helical materials...
- In vivo diagnosis of epidermal growth factor receptor expression using molecular imaging with a cocktail of optically labeled monoclonal antibodiesTristan Barrett
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Clin Cancer Res 13:6639-48. 2007..Here, we use a "cocktail" of optically labeled monoclonal antibodies directed against EGFR-1 (HER1) and EGFR-2 (HER2) to distinguish tumors by their cell surface expression profiles...
- Fluorescence tomography and magnetic resonance imaging of myocardial macrophage infiltration in infarcted myocardium in vivoDavid E Sosnovik
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Circulation 115:1384-91. 2007....
- In vivo detection of head and neck cancer orthotopic xenografts by immunofluorescenceEben L Rosenthal
Department of Surgery, Division of Otolaryngology Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA
Laryngoscope 116:1636-41. 2006..To determine whether Cy5.5-labeled antiepidermal growth factor (EGFR) antibody could be used to detect head and neck squamous cell carcinoma (HNSCC) xenografts in vivo...
- Prospective isolation of late development multipotent precursors whose migration is promoted by EGFRFrancesca Ciccolini
Department of Neurobiology, Interdisciplinary Center for Neurosciences IZN, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany
Dev Biol 284:112-25. 2005..In addition, it can be used as a marker to effectively isolate telencephalic multipotent precursors from embryonic and adult tissue...
- Diagnosis of arthritis using near-infrared fluorochrome Cy5.5Andreas Hansch
Institute of Diagnostic and Interventional Radiology, Friedrich Schiller University Jena, Jena, Germany
Invest Radiol 39:626-32. 2004..This applicability study sought to define whether imaging with fluorochrome Cy5.5 can identify arthritis in murine antigen-induced arthritis (AIA)...
- In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in brain tumor xenograftsXiaoyuan Chen
PET Imaging Science Center, University of Southern California Keck School of Medicine, Los Angeles, California, USA
Cancer Res 64:8009-14. 2004....
- Targeting gelatinases with a near-infrared fluorescent cyclic His-Try-Gly-Phe peptideWei Wang
Department of Experimental Diagnostic Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
Mol Imaging Biol 11:424-33. 2009..The purpose of the study is to synthesize and characterize near-infrared (NIR) fluorescence imaging probes targeted to gelatinases...
- Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidinH J Gruber
Institute of Biophysics and Institute of Chemistry, J Kepler University, Altenberger Strasse 69, A 4040 Linz, Austria
Bioconjug Chem 11:696-704. 2000..5, were similar to Cy5. In conclusion the Cy dye series is divided into two groups: Antibodies with multiple Cy3 or Cy3.5 labels yield bright fluorescence while extensive quenching occurs in antibodies labeled with Cy5 and Cy7...
- Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopyV N Dedov
Department of Pharmacy, University of Sydney, NSW, 2006, Syndey, Australia
Micron 32:653-60. 2001....
- Accumulation of liposome with Sialyl Lewis X to inflammation and tumor region: application to in vivo bio-imagingMasahiko Hirai
Kobe R and D Center, Katayama Chemical Industries Co, Ltd, Japan
Biochem Biophys Res Commun 353:553-8. 2007..Thus, it was proven that this liposome is useful not only as an in vivo bio-imaging reagent but also as a drug delivery system (DDS)...
- Identification and removal of contaminating fluorescence from commercial and in-house printed DNA microarraysM Juanita Martinez
Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
Nucleic Acids Res 31:e18. 2003....
- Single-pair fluorescence resonance energy transfer (spFRET) for the high sensitivity analysis of low-abundance proteins using aptamers as molecular recognition elementsWonbae Lee
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803 1804, USA
J Fluoresc 20:203-13. 2010..We also demonstrate that the technique can discriminate thrombin molecules from its analogue prothrombin. The analytical sensitivity was >200-fold better than an ensemble measurement...
- 1H NMR and optical spectroscopic investigation of the sequence-dependent dimerization of a symmetrical cyanine dye in the DNA minor grooveKristen C Hannah
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
Biochemistry 44:15924-9. 2005....
- Flow cytometric probing of mitochondrial function in equine peripheral blood mononuclear cellsDominique Cassart
Department of Pathology, Faculty of Veterinary Medicine, University of Liege, Sart Tilman B43, B 4000 Liege, Belgium
BMC Vet Res 3:25. 2007....
- False labelling due to quenching failure of N-hydroxy-succinimide-ester-coupled dyesWeiqun Wang
Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, Medical Faculty, University of Munster, Munster, Germany
Proteomics 10:1525-9. 2010..It could be shown that only a 100-fold increase in the amount of quencher will silence residual dye to such an extent that no artificial signals are detected...
- Suppressive effects of a cyanine dye against herpes simplex virus (HSV)-1 infectionChie Ushio
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan
Biomed Res 30:365-8. 2009..These results suggest that lumin exerts an antiviral action against HSV-1 with the independent pathways of IFN-alpha and also it would become a therapeutically effective drug in clinical practice...
- Experimental and theoretical study of the interaction of single-stranded DNA homopolymers and a monomethine cyanine dye: nature of specific bindingLarisa Mikelsons
Department of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
Photochem Photobiol Sci 4:798-802. 2005....
- Gene-specific dye bias in microarray reference designsAlan A Dombkowski
Institute of Environmental Health Sciences, Wayne State University, 2727 Second Ave, Detroit, MI 48201, USA
FEBS Lett 560:120-4. 2004..We demonstrate that this assumption is not reliable and that dye orientation can significantly influence measured changes in gene expression...
- In vivo imaging of experimental arthritis with near-infrared fluorescenceAndreas Hansch
Institute of Diagnostic and Interventional Radiology, Fredrich Schiller University Jena, Jena, Germany
Arthritis Rheum 50:961-7. 2004..To visualize early experimental arthritis with near-infrared fluorescence (NIRF) imaging in a murine model of antigen-induced arthritis (AIA)...
- Kinetic mechanism of direct transfer of Escherichia coli SSB tetramers between single-stranded DNA moleculesAlexander G Kozlov
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, 660 S Euclid Avenue, St Louis, MO 63110, USA
Biochemistry 41:11611-27. 2002..Such a mechanism may be used to recycle SSB tetramers between old and newly formed ssDNA regions during lagging strand DNA replication...
- A new method for in vitro detection of microbially produced mitochondrial toxinsD Hoornstra
University of Helsinki, Department of Applied Chemistry and Microbiology, Division of Microbiology, PO Box 56, FIN 00014 University of Helsinki, Finland
Toxicol In Vitro 17:745-51. 2003..Surfactin and lichenysin A caused mitochondrial damage at concentrations where the plasma membrane was also damaged...
- Vessel painting of the microcirculation using fluorescent lipophilic tracersDino J Ravnic
Laboratory of Immunophysiology, Department of Surgery, Brigham and Women s Hospital, Harvard Surgical Research Laboratories, Harvard Medical School, Room 259, 75 Francis Street, Boston, MA 02115, USA
Microvasc Res 70:90-6. 2005..These studies suggest the utility of fluorescent vessel painting as a complementary technique to corrosion casting in the morphometric study of the microcirculation...
- Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiIYiwen Li
Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, 506 McKnight Building, 1638 NW 10th Avenue, Miami, Florida 33136, USA
Nat Protoc 3:1703-8. 2008....
- Nobiletin, a citrus flavonoid, suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cellsAi Eguchi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
FEBS Lett 580:3321-8. 2006..Together, our results suggest that nobiletin is a promising phytochemical for regulating atherosclerosis with reasonable action mechanisms...
- Fluorescence properties and photophysics of the sulfoindocyanine Cy3 linked covalently to DNAMatthew E Sanborn
Department of Chemistry and Biochemistry, Department of Physics and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287 5601, USA
J Phys Chem B 111:11064-74. 2007..The remarkable variations in the photophysical properties of Cy3-DNA constructs demonstrate that caution should be used when Cy3 is used in studies employing DNA conjugates...
- Model-based analysis of two-color arrays (MA2C)Jun S Song
Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
Genome Biol 8:R178. 2007..The tools have been implemented as a stand-alone Java program called MA2C, which can display various plots of statistical analysis for quality control...
- Intrinsic connectivity of human superior colliculusEric Tardif
Division de Neuropsychologie, CHUV, 1011, Lausanne, Switzerland
Exp Brain Res 166:316-24. 2005..The putative roles of these connections are discussed with regard to visual receptive field organization, as well as visuomotor and multisensory integration...
- Near-infrared fluorescent nanoparticles as combined MR/optical imaging probesLee Josephson
Center for Molecular Imaging Research, Massachusetts General Hospital, Charlestown 02129, USA
Bioconjug Chem 13:554-60. 2002..The probes may provide the basis for a new class of so-called smart nanoparticles, capable of pinpointing their position through their magnetic properties, while providing information on their environment by optical imaging techniques...
- Role reversal in a supramolecular assembly: a chiral cyanine dye controls the helicity of a peptide-nucleic acid duplexBabu Rao Renikuntla
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
Langmuir 21:5362-6. 2005..These results indicate that chirality is transferred from the dye to the PNA, as opposed to other examples of polymer-templated dye aggregation where chirality is transferred from the template to the dye...
- G-quadruplex DNA binding by a series of carbocyanine dyesS M Kerwin
Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, 78712, USA
Bioorg Med Chem Lett 11:2411-4. 2001..Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration...
- Effects of a modified dye-labeled nucleotide spacer arm on incorporation by thermophilic DNA polymerasesChristopher Lacenere
Division of Biology, California Institute of Technology, Pasadena, California, USA
Nucleosides Nucleotides Nucleic Acids 25:9-15. 2006..Furthermore, some of the assayed polymerases are capable of extending the primer to the full or near full length of 30 nucleotides using dye-labeled nucleotides exclusively...
- Chiral transformation of achiral J-aggregates of a cyanine dye templated by human serum albuminYazhou Zhang
Beijing National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, P R China
Chemphyschem 8:224-6. 2007
- In vivo imaging of integrin alpha v beta 3 expression using fluorescence-mediated tomographyAngelika von Wallbrunn
Department of Clinical Radiology, University of Munster, Albert Schweitzer Strasse 33, 48129, Munster, Germany
Eur J Nucl Med Mol Imaging 34:745-54. 2007..We therefore synthesised an alpha v beta(3) targeted fluorochrome and imaged tumour xenografts with different alpha v beta(3) expression levels using both planar and tomographic optical imaging methods...
- Nonblinking and long-lasting single-molecule fluorescence imagingIvan Rasnik
Department of Physics, Emory University, Atlanta, Georgia 30322, USA
Nat Methods 3:891-3. 2006....
- The combined effects of trkB and trkC mutations on the innervation of the inner earB Fritzsch
Creighton University, Department of Biomedical Sciences, Omaha, NE 68178, USA
Int J Dev Neurosci 16:493-505. 1998....
- Location of cyanine-3 on double-stranded DNA: importance for fluorescence resonance energy transfer studiesD G Norman
Department of Biochemistry, The University of Dundee, UK
Biochemistry 39:6317-24. 2000..The knowledge of the fluorophore positions can now be used for more precise interpretation of FRET data from nucleic acids...
- Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurementsWilliam S Branham
Center for Functional Genomics, Division of Systems Toxicology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079, USA
BMC Biotechnol 7:8. 2007..Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy5 signal relative to the Cy3 signal in 2-color microarrays will adversely reduce the Cy5/Cy3 ratio resulting in unreliable microarray data...
- Multiplex detection and quantitation of proteins on western blots using fluorescent probesJ C Gingrich
Bio Rad Laboratories, Hercules, CA, USA
Biotechniques 29:636-42. 2000..Two examples of three-color multiplex detection using FITC-, Cy3- and Cy5-conjugated probes on Western blots are provided to demonstrate applications of this approach...
- Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probesJ B Randolph
Center for Light Microscope Imaging and Biotechnology and Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
Nucleic Acids Res 25:2923-9. 1997..A mechanism that accounts for this destabilization is proposed which is consistent with our data. It involves dye-dye and dye-nucleotide interactions which appear to stabilize a single-stranded conformation of the probe...
- Investigation of DNA binding modes for a symmetrical cyanine dye trication: effect of DNA sequence and structureR Cao
Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213-3890, USA
J Biomol Struct Dyn 18:844-56. 2001..Seifert, J.L., et al. (1999) J. Am. Chem. Soc. 121, 2987-2995] These studies illustrate the diversity of DNA binding modes that are available to a given ligand structure...
- Optimization of oligonucleotide-based DNA microarraysAngela Relógio
Gene Expression Programme and Functional Genomics Technology and Instrumentation Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nucleic Acids Res 30:e51. 2002..These data will facilitate the design and standardization of custom-made microarrays applicable to gene expression profiling and sequencing analyses...
- A multimodal nanoparticle for preoperative magnetic resonance imaging and intraoperative optical brain tumor delineationMoritz F Kircher
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
Cancer Res 63:8122-5. 2003..This prototypical multimodal nanoparticle has unique properties that may allow radiologists and neurosurgeons to see the same probe in the same cells and may offer a new approach for obtaining tumor margins...
- Triple-target microarray experiments: a novel experimental strategyThorsten Forster
Scottish Centre for Genomic Technology and Informatics, University of Edinburgh, The Chancellor s Building, College of Medicine, 49 Little France Crescent, Edinburgh, EH16 4SB, UK
BMC Genomics 5:13. 2004....
- Live imaging of mammalian retina: rod outer segments are stained by conventional mitochondrial dyesPaolo Bianchini
University of Genoa, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy LAMBS, MicroSCoBiO Research Center, Department of Physics, Via Dodecaneso 33, 16146, Genoa, Italy
J Biomed Opt 13:054017. 2008..This finding may shed light on previous reports of the existence of a proton potential across the disk membranes and on the mechanism of the adenosine tri-phosphate (ATP) supply for phototransduction, which still requires investigation...
- Olivary projection from the rostral part of the nucleus of Darkschewitsch in the postnatal rat as revealed through the use of a carbocyanine dyeSatoru Onodera
Department of Anatomy, School of Medicine, Iwate Medical University, Uchimaru 19 1, Morioka 020 8505, Japan
Brain Res 1015:194-7. 2004..The present study suggests the existence of a precise topographic organization from subnuclei of the area parafascicularlis prerublaris to subnuclei of the inferior olive...
- Intensity-based analysis of two-colour microarrays enables efficient and flexible hybridization designsPeter A C 't Hoen
Center for Human and Clinical Genetics, Leiden University Medical Center, The Netherlands
Nucleic Acids Res 32:e41. 2004....
- An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experimentsColin Echeverría Aitken
Biophysics Program, Stanford University School of Medicine, Stanford, California 94305 5126, USA
Biophys J 94:1826-35. 2008..We observed increased stability of Cy3 and Alexa488 in the presence of the antioxidants ascorbic acid and n-propyl gallate. This new O(2)-scavenging system should have wide application for single-molecule fluorescence experiments...
- Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particlesJoanna Malicka
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA
Anal Biochem 317:136-46. 2003..These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays...
- Effects of atmospheric ozone on microarray data qualityThomas L Fare
Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA
Anal Chem 75:4672-5. 2003..To address this environmental effect, laboratory ozone levels should be kept below 2 ppb (e.g., with filters in HVAC) to achieve high quality microarray data...
- Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotidesJoanna Malicka
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Anal Biochem 315:57-66. 2003..These results suggest that maximum fluorescence enhancements occur about 90A from the silver surface, a distance readily obtained by one or two layers of proteins...
- Comparison of two tricarbocyanine-based dyes for fluorescence optical imagingChristin Perlitz
Optical Imaging and New Modalities Research, Research Laboratories, Schering AG, Müllerstrasse, 13342 Berlin, Germany
J Fluoresc 15:443-54. 2005..The primary aim was to modify the physicochemical properties of the previously synthesized dye SIDAG to investigate the effect on the in vivo imaging properties...
- Integrated Instrumentation for ProteomicsHelen Kim; Fiscal Year: 2002....
- Metal-Enhanced Fluorescence SensingJoseph Lakowicz; Fiscal Year: 2006..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
- Annual Meeting of the NCRR P41 Principal InvestigatorsJoseph Lakowicz; Fiscal Year: 2005..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
- NOVEL TYROSINASE MUTANTS AS IMAGING MARKER GENESAlexei Bogdanov; Fiscal Year: 2002..The long-term goal of this research is to explore and develop novel and useful bifunctional "imaging/therapeutic marker genes" for in vivo use. ..
- Transcription Factor Reporter TechnologyAlexei Bogdanov; Fiscal Year: 2006..abstract_text> ..
- CELLULAR PATHOPHYSIOLOGY OF ACUTE RENAL FAILUREJoel Weinberg; Fiscal Year: 2007..abstract_text> ..
- Biohazard Detection Using Metal-Enhanced FluorescenceJoseph Lakowicz; Fiscal Year: 2003..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
- CFS Course on Fluorescence SpectroscopyJoseph Lakowicz; Fiscal Year: 2006..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
- STRUCTURE AND PLASTICITY OF CENTRAL HEARING MECHANISMSCRAIG HENKEL; Fiscal Year: 2002..Results should lead to a better understanding of the influence of experience on the development of these binaural sensory pathways. ..
- HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QAMartin Hessner; Fiscal Year: 2006..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
- Dissection of cellular interactions with integrated functional genomics in compleMartin Hessner; Fiscal Year: 2008..Understanding the relationship between mast cells, T effector, and TREG cells in autoimmunity and has the potential to establish a new paradigm for immune regulation and create new insights to human disease. ..
- CENTER FOR FLUORESCENCE SPECTROSCOPYJoseph Lakowicz; Fiscal Year: 2006..Abstract Not Provided. ..
- HEARING: DEVELOPMENT OF SYNAPTIC CIRCUITSCRAIG HENKEL; Fiscal Year: 2005....
- A role for dopamine in brain developmentPradeep Bhide; Fiscal Year: 2007..It is also an excellent model to mimic dopamine imbalance in the CNS that can be produced by therapeutic drugs that intentionally or unintentionally target the dopaminergic system of the developing brain. ..
- Membrane protein electrophoresis in lipid bilayersMary Wirth; Fiscal Year: 2006..If feasible, an R01 proposal would address separations of real mixtures of membrane proteins from cell lysates. The work could have enormous impact on drug discovery, allowing inexpensive screening that encompasses many receptors. ..
- Non-Radial Cell Migration in CNS DevelopmentJeffrey A Golden; Fiscal Year: 2010..abstract_text> ..
- SINGLE-MOLECULE ANALYSIS OF A TRANSCRIPTION ENHANCERLarry Friedman; Fiscal Year: 2006..The single-molecule approach will highlight relations between the mechanisms at successive steps, thus contributing to an integrated description of the enhancer-mediated initiation process in this model system. ..
- Workshop on Molecular Imaging AgentsDavid Vera; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable]..
- Role of the Foxn4 Gene during RetinogenesisMengqing Xiang; Fiscal Year: 2007..abstract_text> ..
- Pulmonary Mitochondrial Injury Caused by TetrahydrocannabinolTheodore Sarafian; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- MULTIMODAL DOT-PET MOLECULAR PROBES AND TANDEM SYSTEMJoseph P Culver; Fiscal Year: 2010..abstract_text> ..
- Effects of Methylmercury on Neuronal Cell MigrationHitoshi Komuro; Fiscal Year: 2010..Answers to the questions raised in this project will provide insights for development of therapies that counter MeHg-induced brain malformation. ..
- Tissue-Specific Optical Imaging Agents and MethodsSamuel Achilefu; Fiscal Year: 2007..Products of this study will also be made available to other investigators interested in molecular-probe- mediated optical imaging and related medical/pharmaceutical applications. ..
- A new general methodology for studying conformational dynamics in single cellsMarcia Levitus; Fiscal Year: 2008..Understanding chromatin dynamics is a key step in the process of elucidating the mechanisms of development, differentiation and cancer. [unreadable] [unreadable] [unreadable]..
- Optical Probes & Methods for Imaging Integrin ExpressionSamuel Achilefu; Fiscal Year: 2008..All these products will be made available to other investigators. ..
- AAV Vector Targeting Strategies for Gene TherapyJeffrey Bartlett; Fiscal Year: 2008..abstract_text> ..
- CYTOMETRIC CD4 COUNTING - A MINIMALIST APPROACHHoward Shapiro; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Interactions of Lipoproteins with the Vascular WallJOHN CALVERT RUTLEDGE; Fiscal Year: 2010....
- ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE AND SURGERY CONFERENCESamuel Achilefu; Fiscal Year: 2007..unreadable] Aim 4: To promote interaction between the invited scientists and participating students, and to enable [unreadable] collaborations. [unreadable] [unreadable] [unreadable]..
- Ultrasound Monitoring of Rat Metastatic Uveal MelanomaRODNEY BRAUN; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- INIA: NeurohistologyANDREA ELBERGER; Fiscal Year: 2006..The final product produced by the Core will be tissue sections on microscope slides with appropriate coding to track each case. A database will be maintained in the Core to assure accuracy of data analyses. ..
- CELL MIGRATION AND CNS DEVELOPMENT ANOMALIESJeffrey Golden; Fiscal Year: 2002..abstract_text> ..
- BLOOD POOL CONTRAST AGENTS FOR CT AND MRIDavid Vera; Fiscal Year: 2002..Additionally, this structure will serve as a neural carrier of for future class of nonparticulate receptor-binding CT and MR contrast media. ..
- DEVELOPMENT OF THE OLFACTORY SYSTEM IN ZEBRAFISHKATHLEEN WHITLOCK; Fiscal Year: 2004..abstract_text> ..
- CONFORMATIONALLY ASSISTED FET PROBESRonald Cook; Fiscal Year: 2002..It is estimated that probe cost will diminish from the current average of $400 per probe to less than $100 per probe, thus opening quenched probes to entirely new applications and markets. ..
- DESIGN, SYNTHESIS AND STUDY OF NEW SENSITIZERS FOR PDTMICHAEL DETTY; Fiscal Year: 2004....
- HIGH-PRECISION FLOW CYTOMETRY OF BACTERIA AND VIRUSESHoward Shapiro; Fiscal Year: 2004..The relationship between staining intensity and nucleic acid type and content in viruses, will be investigated, with a view toward refining cytometric virus detection and identification. ..
- MicroPET and NIR Fluorescence Imaging Tumor AngiogenesisXiaoyuan Chen; Fiscal Year: 2004..All the results obtained here will be used for future application of a R01 type grant. ..
- Excitotoxic Signaling in HD Transgenic NeuronsKARI HOYT; Fiscal Year: 2004..The cell culture models of HD proposed in this work should also provide a convenient means for testing relevant therapies for HD. ..
- High Performance Lanthanide DNA ProbesRonald Cook; Fiscal Year: 2002..As a result the practical and economic synthesis of lanthanide oligo probes will become possible, and their application to genomic assays can begin. ..
- Effects of alcohol on neuronal cell migrationHitoshi Komuro; Fiscal Year: 2005..Answers to the questions raised in this project will provide a new insight for understanding how prenatal and early postnatal exposure to alcohol causes malformation of brain. ..
- Roles of Homeodomain Factors in Neural DevelopmentMengqing Xiang; Fiscal Year: 2004....
- Hybrid optical-ultrasound scanner for cancer imagingVasilis Ntziachristos; Fiscal Year: 2005..abstract_text> ..
- Mechanisms of Auto-Rhythmicity in Heart DevelopmentMARGARET KIRBY; Fiscal Year: 2005..The results from the completion of the proposed experiments will lead to a greater understanding of cardiac arhythmias in human heart disease and facilitate the design of treatment strategies. ..
- A Novel Immunotoxin for Treatment and Cure of StrabismusLEO CHALUPA; Fiscal Year: 2005..The use of IX-146 could be extended in the future to a wider variety of ophthalmologic, neurologic, and neuromuscular disorders, including blepharospasm as well as other types of dystonias. ..
- NMR AND FUNCTIONAL ANALYSIS OF ONCOGENE PRODUCTSMarkus Germann; Fiscal Year: 2001..The solution structure is of particular relevance for the design of agents that act as inhibitors of oncogene function. Inhibitors of hTCL-1 and hMTCP-1 have potential for treatment of low-grade T-cell lymphomas and leukemias. ..
- ROLES OF NITRIC OXIDE AND OXYGEN IN OCULAR MELANOMARODNEY BRAUN; Fiscal Year: 2002....
- NEUROPHYSIOLOGY OF CRANIAL HEADACHEAndrew Strassman; Fiscal Year: 2008..This information can then be used to develop strategies for blocking the neural changes that result in headache. [unreadable] [unreadable]..
- Imaging of gene expression in glioblastomaJAMES PETER BASILION; Fiscal Year: 2010..The conceptual scheme presented herein is also potentially applicable to a variety of diseases for which gene profiling analyses is available. ..
- Symposium on Imaging Agents and Molecular BeaconsSamuel Achilefu; Fiscal Year: 2004..Five sessions will be held and will cover topics on nuclear, magnetic resonance, ultrasound, radiographic, optical and miscellaneous imaging agents for medical applications. ..
- DNA-based Arrays of Cross-reactive Molecular SensorsMilan Stojanovic; Fiscal Year: 2004..In the next phase, DNA-based cross-reactive sensors for oligosaccharides will be incorporated into arrays to expand the panel of metabolites that could be profiled in a single-step assay. ..
- NMR STRUCTURE OF ZINC FINGERS SPECIFIC FOR HIV RRE RNAMarkus Germann; Fiscal Year: 2005..The inhibitory activity of designed zinc fingers will be determined through in vitro and in vivo assays. ..
- DEVELOPMENT OF THE GUSTATORY SYSTEMRICHARD NORTHCUTT; Fiscal Year: 2005..To test the hypothesis that retinoic acid acts as a signal to pattern endoderm directly during gastrulation (aim 3), presumptive pharyngeal endoderm will be excised, exposed to retinoic acid, and cultured. ..
- High Performance Lanthanide DNA ProbesRonald Cook; Fiscal Year: 2005..We will seek to take advantage of the long fluorescence lifetimes of lanthanide reporters by employing "gated" detection of lanthanide fluorescence under thermocycling conditions for PCR assays. ..
- Protein ubiquitination based neuroprotection for strokeRobert Meller; Fiscal Year: 2006....
- Genetic remodeling of Vagal Afferent OrganizationEdward Fox; Fiscal Year: 2009..Moreover, it will form an essential foundation for further research on the development of, and the structural and functional organization of, the diverse systems that form the vagal sensorium. ..
- Layer-by-layer assembly for making drug-eluting stentsKinam Park; Fiscal Year: 2007..unreadable] [unreadable]..
- Rapid ischemic tolerance: Ubiquitin-mediated structural reorganizationRobert Meller; Fiscal Year: 2007..Indeed, the long-term aim of these studies is to discover endogenous protective mechanisms that can be translated into effective rapid acting neuroprotective agents for stroke. [unreadable] [unreadable] [unreadable]..
- Microenvironment-Controlled Encapsulation (MICE) ProcessKinam Park; Fiscal Year: 2005..The MICE process will facilitate microencapsulation of a variety of protein drugs that have been difficult to formulate with the conventional methods, as well as small molecular weight drugs. ..
- Solvent exchange methods for protein microencapsulationKinam Park; Fiscal Year: 2006..Because of the simplicity of the solvent exchange method, it is expected to open a new avenue of microencapsulation of protein drugs with various properties. ..
- Hydrotropic Polymers for oral delivery of paclitaxelKinam Park; Fiscal Year: 2008..The oral paclitaxel formulations are expected to have great therapeutic potential not only for cancer treatment as well as neurodegenerative diseases that can be potentially treated by paclitaxel. ..
- RADIOIMMUNOTHERAPY OF B CELL LYMPHOMA WITH ANTI CD74M Mattes; Fiscal Year: 2002..This study may lead to the development of more effective radio-conjugates for the therapy of cancer and other diseases. Also, it will provide basic information about the toxic effects of intracellular (really lysosomal) radionuclides. ..