A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activityCristobal Bernal
Departament de Bioquimica i Biologia Molecular, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain
Anal Biochem 340:245-51. 2005
....
Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coliAnnik Nanchen
Institute of Molecular Systems Biology, ETH Zurich, CH 8093 Zurich, Switzerland
Appl Environ Microbiol 72:1164-72. 2006
..this glyoxylate shunt activity is best understood from a network perspective as the recently described phosphoenolpyruvate (PEP)-glyoxylate cycle that oxidizes PEP (or pyruvate) to CO2...
- Structure of phosphorylated enzyme I, the phosphoenolpyruvate:sugar phosphotransferase system sugar translocation signal protein
Alexey Teplyakov
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 103:16218-23. 2006
Bacterial transport of many sugars, coupled to their phosphorylation, is carried out by the phosphoenolpyruvate (PEP):sugar phosphotransferase system and involves five phosphoryl group transfer reactions...
- 3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate
T Wagner
Department of Biology, University of Virginia, Charlottesville, VA, 22903 2477, USA
J Mol Biol 301:233-8. 2000
..coli KDOPS, grown in 24 % (w/v) polyethylene glycol (PEG) 1500 in the presence of the substrates, 2-phosphoenolpyruvate (PEP) and d-arabinose-5-phosphate (A5P), are also in space group I23 (a=118...
- Opposing effects of phosphoenolpyruvate and pyruvate with Mg(2+) on the conformational stability and dimerization of phosphotransferase enzyme I from Escherichia coli
Mariana N Dimitrova
Section on Protein Chemistry, Laboratory of Biochemistry, National Heart, Lung, and Blood Institute NHLBI, National Institutes of Health NIH, Bethesda, Maryland 20892 8012, USA
Protein Sci 12:2047-56. 2003
..The binding of PEP to EI(H189A) is synergistic with that of Mg(2+). Thus, physiological concentrations of PEP and Mg(2+) increase, whereas pyruvate and Mg(2+) decrease the amount of dimeric, active, dephospho-enzyme I...
- Transient state kinetics of Enzyme I of the phosphoenolpyruvate:glycose phosphotransferase system of Escherichia coli: equilibrium and second-order rate constants for the phosphotransfer reactions with phosphoenolpyruvate and HPr
Norman D Meadow
Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
Biochemistry 44:12790-6. 2005
The first two reactions in the phosphotransfer sequence of bacterial phosphoenolpyruvate:glycose phosphotransferase systems are the autophosphorylation of Enzyme I by phosphoenolpyruvate followed by the transfer of the phospho group to ..
- Molecular characterization of a phosphoenolpyruvate carboxylase from a thermophilic cyanobacterium, Synechococcus vulcanus with unusual allosteric properties
Li-Mei Chen
Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
Plant Cell Physiol 43:159-69. 2002
A gene for phosphoenolpyruvate carboxylase (PEPC) was isolated from a thermophilic cyanobacterium, Synechococcus vulcanus, by screening a genomic DNA library using the coding region of Anacystis nidulans 6301 PEPC as a probe. The S...
- Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution
E Zhang
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA
Biochemistry 36:12526-34. 1997
Enolase, a glycolytic enzyme that catalyzes the dehydration of 2-phospho-d-glycerate (PGA) to form phosphoenolpyruvate (PEP), is a homodimer in all eukaryotes and many prokaryotes...
- Phosphoenolpyruvate provision to plastids is essential for gametophyte and sporophyte development in Arabidopsis thaliana
Veena Prabhakar
Universitat zu Koln, Biozentrum, Botanisches Institut II, D 50674 Cologne, Germany
Plant Cell 22:2594-617. 2010
Restriction of phosphoenolpyruvate (PEP) supply to plastids causes lethality of female and male gametophytes in Arabidopsis thaliana defective in both a phosphoenolpyruvate/phosphate translocator (PPT) of the inner envelope membrane and ..
- Differential regulation of endogenous glucose-6-phosphatase and phosphoenolpyruvate carboxykinase gene expression by the forkhead transcription factor FKHR in H4IIE-hepatoma cells
A Barthel
Institut fur Pharmakologie und Toxikologie, RWTH Aachen, Aachen, D 52057, Germany
Biochem Biophys Res Commun 285:897-902. 2001
..H4 cells) was used in order to determine the role of the transcription factor FKHR in the regulation of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase)...
- Glucose transport by a mutant of Streptococcus mutans unable to accumulate sugars via the phosphoenolpyruvate phosphotransferase system
D G Cvitkovitch
Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada
J Bacteriol 177:2251-8. 1995
Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS)...
- A global regulatory role of gluconeogenic genes in Escherichia coli revealed by transcriptome network analysis
Katy C Kao
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, USA
J Biol Chem 280:36079-87. 2005
..These results suggest that the gluconeogenic flux to phosphoenolpyruvate is important for full activation of adenylate cyclase through the phosphorylated enzyme IIA(glu) of the ..
- Phosphoenolpyruvate-dependent tubulin-pyruvate kinase interaction at different organizational levels
Janos Kovacs
Department of General Zoology, , P.O. Box 330, H-1445 Budapest, Hungary
J Biol Chem 278:7126-30. 2003
Evidence for the direct binding of pyruvate kinase to tubulin/microtubule and for the inhibitory effect of phosphoenolpyruvate on tubulin-enzyme hetero-association were provided by surface plasmon resonance and pelleting experiments...
- The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA
Rosa Viana
Biotechnology Department, , IATA-CSIC, P.O. Box 73, 46100 Burjassot, Valencia, Spain
Arch Microbiol 183:385-93. 2005
..casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L...
- Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site
Pete Dunten
Roche Research Center, Hoffmann La Roche Inc, Nutley, NJ 07110, USA
J Mol Biol 316:257-64. 2002
We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates...
- The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression
S J Streatfield
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
Plant Cell 11:1609-22. 1999
..In this study, we determine that CUE1 encodes the plastid inner envelope phosphoenolpyruvate/phosphate translocator (PPT) and define amino acid residues that are critical for translocator function...
- First characterization of an archaeal GTP-dependent phosphoenolpyruvate carboxykinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1
Wakao Fukuda
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo ku, Kyoto 615 8510, Japan
J Bacteriol 186:4620-7. 2004
b>Phosphoenolpyruvate carboxykinase (PCK), which catalyzes the nucleotide-dependent, reversible decarboxylation of oxaloacetate to yield phosphoenolpyruvate and CO2, is one of the important enzymes in the interconversion between C3 and C4 ..
- Pathway engineering for the production of aromatic compounds in Escherichia coli
N Flores
Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
Nat Biotechnol 14:620-3. 1996
..formation of glucose-6-phosphate through the glucose phosphotransferase system (PTS), one molecule of phosphoenolpyruvate (PEP) is consumed...
- Inducer exclusion in Escherichia coli by non-PTS substrates: the role of the PEP to pyruvate ratio in determining the phosphorylation state of enzyme IIAGlc
B M Hogema
E C Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
Mol Microbiol 30:487-98. 1998
..repression in Escherichia coli is the dephosphorylation of enzyme IIAGlc, one of the enzymes of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS)...
- Nutrient-scavenging stress response in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system, as explored by gene expression profile analysis
Salvador Flores
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mexico
J Mol Microbiol Biotechnol 10:51-63. 2005
The physiological role of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) has been studied in Escherichia coli...
- Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS)
C Siebold
, , CH-3012, Bern, Switzerland
FEBS Lett 504:104-11. 2001
..Purified, cyclized IIA(Glc) and IICB(Glc) had 100% and 30% of wild-type glucose phosphotransferase activity, respectively. Cyclized IIA(Glc) displayed increased stability against temperature and GuHCl-induced unfolding...
- Cloning and in vivo expression of functional triose phosphate/phosphate translocators from C3- and C4-plants: evidence for the putative participation of specific amino acid residues in the recognition of phosphoenolpyruvate
K Fischer
Julius von Sachs Institut für Biowissenschaften der Universität Würzburg, Germany
Plant J 5:215-26. 1994
..between the recombinant translocator proteins from the two cell types with respect to the transport of phosphoenolpyruvate. Comparison of the cTPT sequences from F. pringlei and F...
- Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase of Thermoproteus tenax: key pieces in the puzzle of archaeal carbohydrate metabolism
Britta Tjaden
Department of Microbiology, Universitat Duisburg Essen, 45117 Essen, Germany
Mol Microbiol 60:287-98. 2006
The interconversion of phosphoenolpyruvate and pyruvate represents an important control point of the Embden-Meyerhof-Parnas (EMP) pathway in Bacteria and Eucarya, but little is known about this site of regulation in Archaea...
- Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose limitation in Escherichia coli
Annik Nanchen
Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
J Bacteriol 190:2323-30. 2008
..most relevant control mechanism was cyclic AMP (cAMP)-dependent catabolite repression as the inducer of the phosphoenolpyruvate (PEP)-glyoxylate cycle and thus low tricarboxylic acid cycle fluxes...
- pH Dependence of the reaction catalyzed by avian mitochondrial phosphoenolpyruvate carboxykinase
Todd Holyoak
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
Biochemistry 43:7054-65. 2004
The pH dependence of the reaction catalyzed by phosphoenolpyruvate carboxykinase (PEPCK) provides significant insight into the chemical mechanism...
- A new class of plastidic phosphate translocators: a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter
K Fischer
Botanisches Institut, Universitat zu Koln, Germany
Plant Cell 9:453-62. 1997
..transport of inorganic phosphate in exchange with C3 compounds phosphorylated at C-atom 2, particularly phosphoenolpyruvate, which is required inside the plastids for the synthesis of, for example, aromatic amino acids...
- Photosynthesis in phosphoenolpyruvate carboxykinase-type C4 plants: pathways of C4 acid decarboxylation in bundle sheath cells of Urochloa panicoides
J N Burnell
Division of Plant Industry, CSIRO, Canberra, Australia
Arch Biochem Biophys 260:187-99. 1988
..mechanism of C4 acid decarboxylation was studied in bundle sheath cell strands from Urochloa panicoides, a phosphoenolpyruvate carboxykinase (PCK)-type C4 plant...
- Lack of glyconeogenesis in pancreatic islets: expression of gluconeogenic enzyme genes in islets
M J MacDonald
Childrens Diabetes Center, University of Wisconsin Medical School, Madison
Horm Metab Res 24:158-60. 1992
..pyruvate carboxylase mRNA, but even islets from rats which had been starved (a condition which induces phosphoenolpyruvate carboxykinase (PEPCK) in liver, kidney and adipose tissue) showed no PEPCK mRNA...
- Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr
J Reizer
Department of Biology, University of California at San Diego, La Jolla 92093 0116, USA
J Bacteriol 178:5480-6. 1996
..We here demonstrate that mutation of His-15 of HPr, the site of phosphoenolpyruvate-dependent phosphorylation, also prevents carbon catabolite repression of the gnt operon...
- A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli
Eliane Fischer
Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
J Biol Chem 278:46446-51. 2003
....
- Structure of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli: comparison of the Mn(2+)*2-phosphoglycolate and the Pb(2+)*2-phosphoenolpyruvate complexes and implications for catalysis
T Wagner
Department of Biology, University of Virginia, Charlottesville, VA 22903 2477, USA
J Mol Biol 301:389-99. 2000
..distorted octahedral coordination sphere of the metal is completed by PGL, which replaces the substrate, 2-phosphoenolpyruvate (PEP), in the active site...
- Phosphoenolpyruvate availability and the biosynthesis of shikimic acid
Sunil S Chandran
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824 1322, USA
Biotechnol Prog 19:808-14. 2003
The impact of increased availability of phosphoenolpyruvate during shikimic acid biosynthesis has been examined in Escherichia coli K-12 constructs carrying plasmid-localized aroF(FBR) and tktA inserts encoding, respectively, feedback-..
- Streptomyces olivaceoviridis possesses a phosphotransferase system that mediates specific, phosphoenolpyruvate-dependent uptake of N-acetylglucosamine
F Wang
Fachbereich Biologie/Chemie, , Barbarastr. 11, , Germany
Mol Genet Genomics 268:344-51. 2002
..This phenotype can now be attributed to a functional phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS)...
- Phosphoenolpyruvate cycling via mitochondrial phosphoenolpyruvate carboxykinase links anaplerosis and mitochondrial GTP with insulin secretion
Romana Stark
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 284:26578-90. 2009
..Here we have investigated whether the mitochondrial isoform of phosphoenolpyruvate carboxykinase (PEPCK-M) is the GTPase linking hydrolysis of mtGTP made by succinyl-CoA synthetase (SCS-GTP) ..
- Spatial stochastic modelling of the phosphoenolpyruvate-dependent phosphotransferase (PTS) pathway in Escherichia coli
J Vidal Rodríguez
Section Computational Science, Faculty of Science, University of Amsterdam Kruislaan 403, 1098 SJ Amsterdam, The Netherlands
Bioinformatics 22:1895-901. 2006
..Pathways usually consist of a complex interaction network and are characterized by a large set of parameters. The inclusion of spatial and stochastic effects is a major challenge in developing quantitative and dynamic models of pathways...
Acetate metabolism in Escherichia coli strains lacking phosphoenolpyruvate: carbohydrate phosphotransferase system; evidence of carbon recycling strategies and futile cyclesJuan Carlos Sigala
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca Morelos, Mexico
J Mol Microbiol Biotechnol 16:224-35. 2009
..recovered its growth capacity on glucose by an adaptive evolution process (PB12, PTS(-)Glc(+)), part of the phosphoenolpyruvate not used in glucose transport has been utilized for the synthesis of aromatic compounds...
- How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli
Susanne Eschenburg
Max Planck Institute for Medical Research, Department of Biophysics, 69120 Heidelberg, Germany
Planta 216:129-35. 2002
..substrate, shikimate-3-phosphate (S3P), but displays a 30-fold lower affinity for its second substrate, phosphoenolpyruvate (PEP)...
- Analysis of global control of Escherichia coli carbohydrate uptake
Andreas Kremling
Max Planck Institut Magdeburg, Systems Biology, Sandtorstr, 1, 39106 Magdeburg, Germany
BMC Syst Biol 1:42. 2007
..A detailed understanding of the coordination of the control of individual transporters offers possibilities to explore the potential of microorganisms e.g. in biotechnology...
- New bioreactor-coupled rapid stopped-flow sampling technique for measurements of metabolite dynamics on a subsecond time scale
Stefan Buziol
Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany
Biotechnol Bioeng 80:632-6. 2002
....
- Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase
Jian Wang
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
J Mol Biol 324:205-14. 2002
..8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) by favoring the activation of a water molecule coordinated to the active-site metal ion...
- Influence of inorganic pyrophosphate on the kinetics of muscle pyruvate kinase: a simple nonallosteric feedback model
Marian Kuczek
Department of Experimental and Applied Biology, University of Opole, Kominka 4, Poland
Biosystems 66:11-20. 2002
..According to this model, mutual and simultaneous interactions of reaction products with substrates and with an enzyme result in the coexistence of simple and mixed, labile and inert complexes...
- A spectrophotometric assay for quantitative determination of kcat of herpes simplex virus type 1 thymidine kinase substrates
P Schelling
Department of Applied BioSciences, Swiss Federal Institute of Technology (ETH, Winterthurerstrasse 190, , CH-8057, Switzerland
Anal Biochem 295:82-7. 2001
..Exploiting this correlation, this method has been used to determine rapidly and precisely the catalytic turnover constant of antiviral lead compounds not readily available in the radioactive labeled form...
- MgATP-dependent activation by phosphoenolpyruvate of the E187A mutant of Escherichia coli phosphofructokinase
A S Pham
Division of Pathology and Laboratory Medicine, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
Biochemistry 40:4150-8. 2001
..we performed a linkage analysis of the three-ligand interaction of fructose 6-phosphate (Fru-6-P), phosphoenolpyruvate (PEP), and MgATP on E187A mutant Escherichia coli phosphofructokinase (PFK)...
- Allosteric activation of pyruvate kinase via NAD+ in rat liver cells
A Devin
NIH, NCI, Bldg 10, Room 6 N105, Bethesda, MD 20892, USA
Eur J Biochem 268:3943-9. 2001
....
- Human erythrocyte pyruvate kinase: characterization of the recombinant enzyme and a mutant form (R510Q) causing nonspherocytic hemolytic anemia
C Wang
Department of Biochemistry, University of Pavia, Pavia, Italy
Blood 98:3113-20. 2001
..properties characteristic of authentic human erythrocyte pyruvate kinase, including response to substrate, phosphoenolpyruvate, activation by fructose 1,6-bisphosphate, and inhibition by adenosine triphosphate (ATP)...
- Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis
J Wang
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Biochemistry 40:15676-83. 2001
..3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with phosphoenolpyruvate (PEP) and ribose 5-phosphate (R5P), and with a bisubstrate inhibitor that mimics the postulated linear ..
- Three-dimensional structures of protein-protein complexes in the E. coli PTS
A Peterkofsky
Laboratory of Biochemical Genetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 4036, USA
J Mol Microbiol Biotechnol 3:347-54. 2001
The bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) includes a collection of proteins that accomplish phosphoryl transfer from phosphoenolpyruvate (PEP) to a sugar in the course of transport...
- Kinetic linked-function analysis of the multiligand interactions on Mg(2+)-activated yeast pyruvate kinase
T J Bollenbach
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
Biochemistry 40:13097-106. 2001
..These results lend support to the hypothesis that divalent metals modulate the interactions of ligands on YPK and that divalent metals play a role in regulation of the glycolytic pathway...
- Molecular and regulatory properties of leucoplast pyruvate kinase from Brassica napus (rapeseed) suspension cells
William C Plaxton
Department of Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
Arch Biochem Biophys 400:54-62. 2002
..from Brassica napus suspension cells was purified 431-fold to a final specific activity of 28 micromol phosphoenolpyruvate (PEP) utilized/min/mg protein...
- Modification of human erythrocyte pyruvate kinase by an active site-directed reagent: bromopyruvate
N L Acan
Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey
J Enzyme Inhib 16:457-64. 2001
..When the enzyme was modified with bromopyruvate in the absence of adenosine-5'-diphosphate, phosphoenolpyruvate or fructose-1,6-diphosphate the inactivation followed a pseudo first-order kinetics...
- Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes
Luis Fernando Garcia-Alles
Departement fur Chemie und Biochemie, Universitat Bern, Freiestrasse 3, CH 3012 Bern, Switzerland
Eur J Biochem 269:3226-36. 2002
The synthesis of 10 new phosphoenolpyruvate (PEP) analogues with modifications in the phosphate and the carboxylate function is described. Included are two potential irreversible inhibitors of PEP-utilizing enzymes...
- The effect of active site mutations in the oxaloacetate decarboxylase and pyruvate kinase-like activities of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase
Ana María Jabalquinto
Departamento de Ciencias Quimicas, Facultad de Quimica y Biologia, Universidad de Santiago de Chile, Casilla 40, Santiago 33, Chile
J Protein Chem 21:443-5. 2002
Anaerobiospirillum succiniciproducens His225Gln, Asp262Asn, Asp263Asn, and Thr249Asn phosphoenolpyruvate carboxykinases were analyzed for their oxaloacetate decarboxylase, and pyruvate kinase-like activities...
- The use of (E)- and (Z)-phosphoenol-3-fluoropyruvate as mechanistic probes reveals significant differences between the active sites of KDO8P and DAHP synthases
Cristina M Furdui
Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
Biochemistry 44:7326-35. 2005
..DAHP) synthase catalyze a similar aldol-type condensation between phosphoenolpyruvate (PEP) and the corresponding aldose: arabinose 5-phosphate (A5P) and erythrose 4-phosphate (E4P), ..
- Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range
Zhili Li
Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Anal Biochem 343:35-47. 2005
Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of phosphoenolpyruvate (PEP) and D-arabinose 5-phosphate (A5P) to produce KDO8P and inorganic phosphate...
- Phosphoenolpyruvate metabolism in Jerusalem artichoke mitochondria
Lidia de Bari
Istituto di Biomembrane e Bioenergetica, CNR, Via G Amendola 165 A, 70126, Bari, Italy
Biochim Biophys Acta 1767:281-94. 2007
We report here initial studies on phosphoenolpyruvate metabolism in coupled mitochondria isolated from Jerusalem artichoke tubers...
- Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system
Himatkumar V Patel
Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
J Biol Chem 281:17579-87. 2006
The bacterial phosphoenolpyruvate (PEP):glycose phosphotransferase system (PTS) mediates uptake/phosphorylation of sugars. The transport of all PTS sugars requires Enzyme I (EI) and a phosphocarrier histidine protein of the PTS (HPr)...
- The monomer/dimer transition of enzyme I of the Escherichia coli phosphotransferase system
Himatkumar V Patel
Department of Biology, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
J Biol Chem 281:17570-8. 2006
Enzyme I (EI) is the first protein in the phosphotransfer sequence of the bacterial phosphoenolpyruvate:glycose phosphotransferase system. This system catalyzes sugar phosphorylation/transport and is stringently regulated...
- Structure of the full-length enzyme I of the phosphoenolpyruvate-dependent sugar phosphotransferase system
Joséa Márquez
European Molecular Biology Laboratory, Structural and Computational Biology Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
J Biol Chem 281:32508-15. 2006
Enzyme I (EI) is the phosphoenolpyruvate (PEP)-protein phosphotransferase at the entry point of the PEP-dependent sugar phosphotransferase system, which catalyzes carbohydrate uptake into bacterial cells...
- Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily
Celia C H Chen
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
Biochemistry 45:11491-504. 2006
Phosphonopyruvate (P-pyr) hydrolase (PPH), a member of the phosphoenolpyruvate (PEP) mutase/isocitrate lyase (PEPM/ICL) superfamily, hydrolyzes P-pyr and shares the highest sequence identity and functional similarity with PEPM...
- Structural and mechanistic changes along an engineered path from metallo to nonmetallo 3-deoxy-D-manno-octulosonate 8-phosphate synthases
Fathima Kona
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
Biochemistry 46:4532-44. 2007
..These differences may reflect an evolutionary adaptation of metallo and nonmetallo KDO8PS's to the cellular concentrations of these metabolites in their respective hosts...
- Directed evolution of 2-keto-3-deoxy-6-phosphogalactonate aldolase to replace 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase
Ningqing Ran
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA
J Am Chem Soc 129:6130-9. 2007
..synthesis of shikimate pathway products provides an alternate strategy to circumvent the competition for phosphoenolpyruvate between 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) synthase and the phosphoenolpyruvate:..
- How does an enzyme recognize CO2?
Julien J H Cotelesage
Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Sask, Canada
Int J Biochem Cell Biol 39:1204-10. 2007
b>Phosphoenolpyruvate carboxykinase (PCK) reversibly catalyzes the carboxylation of phosphoenolpyruvate to oxaloacetate. Carbon dioxide, and not bicarbonate ion, is the substrate utilized...
- Detection of novel enzyme intermediates in PEP-utilizing enzymes
Karen S Anderson
Department of Pharmacology, SHM B350B, Yale University School of Medicine, 333 Cedar Street New Haven, CT 06520, USA
Arch Biochem Biophys 433:47-58. 2005
..A novel application of this approach using time-resolved electrospray mass spectrometry to detect chemically labile enzyme intermediates is also discussed...
- Proton donor in yeast pyruvate kinase: chemical and kinetic properties of the active site Thr 298 to Cys mutant
Delia Susan-Resiga
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
Biochemistry 43:15230-45. 2004
..The results are consistent with water being the active site proton donor. Active site residue T298 is not critical for activity but plays a role in the activation of the water and affects the pK that modulates catalytic activity...
- Metabolic flux redistribution in Corynebacterium glutamicum in response to osmotic stress
C Varela
Departamento de Ingeniería Química y Bioprocesos, Pontificia Universidad Catolica de Chile, Casilla 306 Correo 22, Santiago, Chile
Appl Microbiol Biotechnol 60:547-55. 2003
..glutamicum...
- Enzyme I of the phosphotransferase system: induced-fit protonation of the reaction transition state by Cys-502
Luis Fernando Garcia-Alles
Departement fur Chemie und Biochemie, Universitat Bern, Switzerland
Biochemistry 42:4744-50. 2003
Enzyme I (EI), the first component of the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS), consists of an N-terminal domain with the phosphorylation site (His-189) and a C-terminal domain with the PEP binding site...
- Identification of the catalytic residues of AroA (Enolpyruvylshikimate 3-phosphate synthase) using partitioning analysis
Shehadeh Mizyed
Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4M1, Canada
Biochemistry 42:6986-95. 2003
AroA (EPSP synthase) catalyzes carboxyvinyl transfer through addition of shikimate 3-phosphate (S3P) to phosphoenolpyruvate (PEP) to form a tetrahedral intermediate (THI), followed by phosphate elimination to give enolpyruvylshikimate 3-..
- Pyruvate kinase: current status of regulatory and functional properties
Ma Enriqueta Muñoz
Facultad de Ciencias Quíicas e Ingenierí, Universidad Autonoma de Baja California, Av Tecnologico s n, Mesa de Otay, B C, Tijuana, Mexico C P 22390
Comp Biochem Physiol B Biochem Mol Biol 135:197-218. 2003
..coli, cat and rabbit muscle. A number of attributes of the enzyme that make it of particular interest in biomedicine and industry are also discussed...
- Metabolic flux analysis of pykF gene knockout Escherichia coli based on 13C-labeling experiments together with measurements of enzyme activities and intracellular metabolite concentrations
K Al Zaid Siddiquee
Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, 820 8502 Fukuoka, Japan
Appl Microbiol Biotechnol 63:407-17. 2004
..To clarify the metabolism quantitatively, it was found to be quite important to integrate the information on intracellular metabolic flux distribution, enzyme activities and intracellular metabolite concentrations...
- Comparative analysis of the Escherichia coli ketopantoate hydroxymethyltransferase crystal structure confirms that it is a member of the (betaalpha)8 phosphoenolpyruvate/pyruvate superfamily
Florian Schmitzberger
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
J Bacteriol 185:4163-71. 2003
..We show that KPHMT forms a family within the phosphoenolpyruvate/pyruvate superfamily...
- Stoichiometry and substrate affinity of the mannitol transporter, EnzymeIImtl, from Escherichia coli
Gertjan Veldhuis
Department of Biochemistry and Biophysical Chemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
Biophys J 89:201-10. 2005
..The dependency could be simulated assuming that >50% of the binding sites were occupied with a ligand that shows an affinity for EnzymeIImtl in the same range as mannitol...
- Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus
Linley R Schofield
Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand
Protein Expr Purif 34:17-27. 2004
..3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAH7PS) catalyzes the condensation reaction between phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P)...
- Substrate and metal complexes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae provide new insights into the catalytic mechanism
Verena König
Department of Structural Chemistry, University of Gottingen, Tammannstr 4, D 37077 Gottingen, Germany
J Mol Biol 337:675-90. 2004
....
- Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis
D M Kim
Department of Chemical Engineering, Stanford University, Stanford, California 94305-5025, USA
Biotechnol Bioeng 74:309-16. 2001
..As a result, the methods described provide cell-free protein synthesis with greater flexibility and cost efficiency...
- Metabolic characterization of Escherichia coli strains adapted to growth on lactate
Qiang Hua
Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093 0412, USA
Appl Environ Microbiol 73:4639-47. 2007
..flux at the pyruvate node remained almost unchanged, while flux distributions around the key metabolites phosphoenolpyruvate, oxaloacetate, and acetyl-coenzyme A were relatively flexible over the course of evolution on lactate to ..
- Plausible phosphoenolpyruvate binding site revealed by 2.6 A structure of Mn2+-bound phosphoenolpyruvate carboxylase from Escherichia coli
H Matsumura
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Japan
FEBS Lett 458:93-6. 1999
We have determined the crystal structure of Mn2+-bound Escherichia coli phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2...
- The catalytic mechanism of EPSP synthase revisited
J Lewis
Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
Biochemistry 38:7372-9. 1999
....
- S-phosphocysteine and phosphohistidine are intermediates in the phosphoenolpyruvate-dependent mannitol transport catalyzed by Escherichia coli EIIMtl
H H Pas
Department of Physical Chemistry, University of Groningen, The Netherlands
Biochemistry 27:5835-9. 1988
..The hydrolysis characteristics of the phosphopeptide indicate that Cys 384 is the site of phosphorylation...
- Cysteine phosphorylation of the glucose transporter of Escherichia coli
M Meins
Department of Microbiology, University of Basel, Switzerland
J Biol Chem 268:11604-9. 1993
..Phosphocysteine has been shown previously to be the catalytic intermediate of the mannitol transporter (Pas, H. H., Meyer, G. H., Kruizinga, W. H., Tamminga, K. S., van Weeghel, R. P., and Robillard, G. T...
- Divergent binding sites in pyruvate kinases I and II from Escherichia coli
G Valentini
Dipartimento di Biochimica, Universita di Pavia
Biol Chem Hoppe Seyler 374:69-74. 1993
Pyridoxal 5'-phosphate incorporation into pyruvate kinase II from E. coli was decreased by the substrate phosphoenolpyruvate and increased by the allosteric activator ribose 5-phosphate, the total incorporation being linearly related to ..
- Catalytic mechanism of Kdo8P synthase: transient kinetic studies and evaluation of a putative reaction intermediate
P H Liang
Department of Chemistry, Technion Israel Institute of Technology, Haifa, Israel
Biochemistry 37:16390-9. 1998
..It was found however that 2 binds to the enzyme and is a competitive inhibitor with respect to phosphoenolpyruvate binding, having a Ki value of 35 microM...
- Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3-phosphate synthase and its activation by univalent cations
W Du
Anti infectives Research, SmithKline Beecham Pharmaceuticals, Collegeville, PA 19426, USA
Eur J Biochem 267:222-7. 2000
..It was shown to catalyze a reversible conversion of shikimate 3-phosphate (S3P) and phosphoenolpyruvate (PEP) to EPSP and inorganic phosphate...
- ADP modulates the dynamic behavior of the glycolytic pathway of Escherichia coli
J C Diaz Ricci
Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas CONICET UNT, Instituto de Química Biológica Dr Bernabe Bloj, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Argentina
Biochem Biophys Res Commun 271:244-9. 2000
..The influence of ADP, phosphoenolpyruvate (PEP), and fructose-6-phosphate (F6P) on the dynamic regulation of this pathway is also analyzed...
- Detection and characterization of a phospholactoyl-enzyme adduct in the reaction catalyzed by UDP-N-acetylglucosamine enolpyruvoyl transferase, MurZ
E D Brown
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
Biochemistry 33:10638-45. 1994
The MurZ enzyme catalyzes enolpyruvoyl transfer from phosphoenolpyruvate to the 3-OH of UDP-N-acetylglucosamine (UDP-GlcNAc) in Escherichia coli peptidoglycan biosynthesis...
- Catalytic role of an arginine residue in the highly conserved and unique sequence of phosphoenolpyruvate carboxylase
M Yano
Faculty of Science, Kyoto University
J Biochem 117:1196-200. 1995
b>Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues...
- "Kinetic competence" of the 5-enolpyruvoylshikimate-3-phosphate synthase tetrahedral intermediate
K S Anderson
Monsanto Agricultural Company, A Unit of Monsanto Company, Technology Division, St Louis, Missouri 63167
J Biol Chem 265:5567-72. 1990
..the active site of 5-enolpyruvoylshikimate-3-phosphate synthase by reaction of shikimate 3-phosphate with phosphoenolpyruvate was isolated, and its properties in solution and in reaction with enzyme were examined...
- Site-directed mutagenesis in the effector site of Escherichia coli phosphofructokinase
F T Lau
Department of Chemistry, Imperial College of Science and Technology, London, U K
Biochemistry 26:4143-8. 1987
..mutagenesis of residue Tyr-55 to assess the role of its side chain in binding an allosteric inhibitor, phosphoenolpyruvate (PEP), and an activator, guanosine 5'-diphosphate (GDP): Tyr-55----Phe-55 and Try-55----Gly-55...
- Phosphoenolpyruvate-dependent flavinylation of 6-hydroxy-D-nicotine oxidase
H Nagursky
Biochemisches Institut, Universitat Freiburg, Federal Republic of Germany
Eur J Biochem 177:319-25. 1988
..It was demonstrated that the reaction required phosphoenolpyruvate (P-pyruvate) in addition to FAD...
- A tetrahedral intermediate in the EPSP synthase reaction observed by rapid quench kinetics
K S Anderson
Monsanto Agricultural Company, A Unit of Monsanto Company, St Louis, Missouri 63167
Biochemistry 27:7395-406. 1988
..3, which is largely a measure of K4.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Capacity of enzymes of the euphorbiacea Aleurites montana involved in CO2-fixation, compared to plants having C3-, C4- and Crassulacean acid metabolism
N Grotjohann
, , , Bundesrepublik Deutschland
Z Naturforsch C 55:383-91. 2000
Capacities of phosphoenolpyruvate carboxylase (PEP-Co), ribulose bisphosphate carboxylase (Rubisco), NADP+ malic enzyme (ME) and of malate dehydrogenase (MDH) were measured in the Euphorbiacea Aleurites montana, grown under 700 ppm CO2 ..
- Physiological consequences of the complete loss of phosphoryl-transfer proteins HPr and FPr of the phosphoenolpyruvate:sugar phosphotransferase system and analysis of fructose (fru) operon expression in Salmonella typhimurium
D A Feldheim
Department of Biology, University of California, San Diego, La Jolla 92093
J Bacteriol 172:5459-69. 1990
..of the fructose operon [fruB(MH)KA], the fructose repressor (fruR), the energy-coupling enzymes of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) (ptsH and ptsI), and the proteins of cyclic AMP action (cya and crp) ..
- The mechanisms by which mild respiratory chain inhibitors inhibit hepatic gluconeogenesis
M R Owen
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK
Biochim Biophys Acta 1142:11-22. 1993
..5 mM. (2) The whole cell concentrations of phosphoenolpyruvate, 2-phosphoglycerate and 3-phosphoglycerate increased about 2-fold, whilst the triose and hexose phosphate ..
- The transcription factor CCAAT/enhancer-binding protein beta regulates gluconeogenesis and phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes
C Arizmendi
Department of Biochemistry and Molecular Biology, University of Salamanca School of Medicine, Salamanca E 37007, Spain
J Biol Chem 274:13033-40. 1999
..C/EBPbeta binds to several sequences of the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter with high affinity, and C/EBPbeta protein is increased 200% in the ..
- Vitamin A regulates genes involved in hepatic gluconeogenesis in mice: phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
D J Shin
Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA
J Nutr 127:1274-8. 1997
..acid (RA) supplementation on regulation of three important genes in hepatic gluconeogenesis: the genes for phosphoenolpyruvate carboxykinase (PEPCK), fructose-1,6-bisphosphatase (Fru-1,6-P2ase) and 6-phosphofructo-2-kinase/fructose-2,..
- Glucose and trehalose PTS permeases of Spiroplasma citri probably share a single IIA domain, enabling the spiroplasma to adapt quickly to carbohydrate changes in its environment
Aurélie André
UMR Génomique Développement et Pouvoir Pathogène, IBVM, Centre INRA de Bordeaux, 71 avenue Edouard Bourlaux, BP 81, 33883 Villenave d Ornon, France
Microbiology 149:2687-96. 2003
..citri phosphoenolpyruvate : glucose and phosphoenolpyruvate : trehalose phosphotransferase systems (PTS) were characterized...
- Regulation of the supply of oxaloacetate for mitochondrial metabolism via phosphoenolpyruvate carboxylase in barley leaf protoplasts. II. Effects of metabolites on PEPC activity at different activation states of the protein
S Kromer
Department of Plant Physiology, University of Umea, Sweden
Biochim Biophys Acta 1289:351-61. 1996
The regulation of the supply of oxaloacetate (OAA) for mitochondrial metabolism via phosphoenolpyruvate carboxylase (PEPC) by metabolites is studied in barley (Hordeum vulgare L...
- Identification of essential histidine residues in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase: analysis by chemical modification with diethyl pyrocarbonate and site-directed mutagenesis
G Y Sheflyan
Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
Biochemistry 38:14320-9. 1999
..from Escherichia coli that catalyzes the aldol-type condensation of D-arabinose 5-phosphate (A 5-P) and phosphoenolpyruvate (PEP) to give KDO 8-P and inorganic phosphate (P(i)) is inactivated by diethyl pyrocarbonate (DEPC)...
- Function of the duplicated IIB domain and oligomeric structure of the fructose permease of Escherichia coli
A Charbit
Department of Biology, University of California at San Diego, La Jolla, California 92093 0116, USA
J Biol Chem 271:9997-10003. 1996
The fructose permease of Escherichia coli, the fructose-specific Enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system (PTS), contains a duplicated IIB domain...
- Inhibition of the phosphoenolpyruvate:lactose phosphotransferase system and activation of a cytoplasmic sugar-phosphate phosphatase in Lactococcus lactis by ATP-dependent metabolite-activated phosphorylation of serine 46 in the phosphocarrier protein HPr
J J Ye
Department of Biology, University of California at San Diego, La Jolla 92093 0116
J Biol Chem 269:11837-44. 1994
..takes up lactose and the nonmetabolizable lactose analogue, thiomethyl-beta-galactoside (TMG), via the phosphoenolpyruvate:sugar phosphotransferase system (PTS) which couples sugar transport to sugar phosphorylation...
- Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria
P W Postma
E C Slater Institute, University of Amsterdam, The Netherlands
Microbiol Rev 57:543-94. 1993
Numerous gram-negative and gram-positive bacteria take up carbohydrates through the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS)...
- Role of the isoforms of CCAAT/enhancer-binding protein in the initiation of phosphoenolpyruvate carboxykinase (GTP) gene transcription at birth
C Croniger
Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4935, USA
J Biol Chem 272:26306-12. 1997
The gene for phosphoenolpyruvate carboxykinase (PEPCK), a target of CCAAT/enhancer-binding protein-alpha (C/EBPalpha) and -beta (C/EBPbeta), begins to be expressed in the liver at birth...
