phosphoenolpyruvate

Summary

Top Publications

  1. ncbi A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity
    Cristobal Bernal
    Departament de Bioquimica i Biologia Molecular, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain
    Anal Biochem 340:245-51. 2005
  2. ncbi The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate
    Nathan P Manes
    Department of Physiology and Biophysics, Stony Brook University, NY 11794 8661, USA
    Arch Biochem Biophys 438:125-36. 2005
  3. pmc Revealing fosfomycin primary effect on Staphylococcus aureus transcriptome: modulation of cell envelope biosynthesis and phosphoenolpyruvate induced starvation
    Marko Petek
    Department of Biotechnology and Systems Biology, National Institute of Biology, Vecna pot 111, Ljubljana, SI 1000, Slovenia
    BMC Microbiol 10:159. 2010
  4. ncbi Photosynthesis in phosphoenolpyruvate carboxykinase-type C4 plants: pathways of C4 acid decarboxylation in bundle sheath cells of Urochloa panicoides
    J N Burnell
    Division of Plant Industry, CSIRO, Canberra, Australia
    Arch Biochem Biophys 260:187-99. 1988
  5. ncbi Phosphoenolpyruvate availability and the biosynthesis of shikimic acid
    Sunil S Chandran
    Department of Chemistry, Michigan State University, East Lansing, Michigan 48824 1322, USA
    Biotechnol Prog 19:808-14. 2003
  6. pmc Effects of phosphorylation on phosphoenolpyruvate carboxykinase from the C4 plant Guinea grass
    Robert P Walker
    Robert Hill Institute and Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, United Kingdom
    Plant Physiol 128:165-72. 2002
  7. ncbi Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus
    Alejandro F Alice
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
    Microbiology 149:1687-98. 2003
  8. pmc Phosphoenolpyruvate carboxykinase as the sole anaplerotic enzyme in Saccharomyces cerevisiae
    Rintze M Zelle
    Department of Biotechnology, Delft University of Technology and Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, Delft, Netherlands
    Appl Environ Microbiol 76:5383-9. 2010
  9. ncbi Cloning and sequencing of two enterococcal glpK genes and regulation of the encoded glycerol kinases by phosphoenolpyruvate-dependent, phosphotransferase system-catalyzed phosphorylation of a single histidyl residue
    V Charrier
    Institut de Biologie et Chimie des Proteines, CNRS, 7 passage du Vercors, F 69367 Lyon Cedex 07, France
    J Biol Chem 272:14166-74. 1997
  10. ncbi Phosphoenolpyruvate, a glycolytic intermediate, as a cytoprotectant and antioxidant in ex-vivo cold-preserved mouse liver: a potential application for organ preservation
    Yuki Kondo
    Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Pharm Pharmacol 65:390-401. 2013

Detail Information

Publications236 found, 100 shown here

  1. ncbi A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity
    Cristobal Bernal
    Departament de Bioquimica i Biologia Molecular, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain
    Anal Biochem 340:245-51. 2005
    ....
  2. ncbi The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate
    Nathan P Manes
    Department of Physiology and Biophysics, Stony Brook University, NY 11794 8661, USA
    Arch Biochem Biophys 438:125-36. 2005
    ..Instead, these phosphorylations and mutations decreased the sensitivity of the 6PF2K to a potent allosteric inhibitor, phosphoenolpyruvate, which appears to be the major regulatory mechanism.
  3. pmc Revealing fosfomycin primary effect on Staphylococcus aureus transcriptome: modulation of cell envelope biosynthesis and phosphoenolpyruvate induced starvation
    Marko Petek
    Department of Biotechnology and Systems Biology, National Institute of Biology, Vecna pot 111, Ljubljana, SI 1000, Slovenia
    BMC Microbiol 10:159. 2010
    ..Fosfomycin is a potent irreversible inhibitor of MurA, an enolpyruvyl transferase that uses phosphoenolpyruvate as substrate...
  4. ncbi Photosynthesis in phosphoenolpyruvate carboxykinase-type C4 plants: pathways of C4 acid decarboxylation in bundle sheath cells of Urochloa panicoides
    J N Burnell
    Division of Plant Industry, CSIRO, Canberra, Australia
    Arch Biochem Biophys 260:187-99. 1988
    ..mechanism of C4 acid decarboxylation was studied in bundle sheath cell strands from Urochloa panicoides, a phosphoenolpyruvate carboxykinase (PCK)-type C4 plant...
  5. ncbi Phosphoenolpyruvate availability and the biosynthesis of shikimic acid
    Sunil S Chandran
    Department of Chemistry, Michigan State University, East Lansing, Michigan 48824 1322, USA
    Biotechnol Prog 19:808-14. 2003
    The impact of increased availability of phosphoenolpyruvate during shikimic acid biosynthesis has been examined in Escherichia coli K-12 constructs carrying plasmid-localized aroF(FBR) and tktA inserts encoding, respectively, feedback-..
  6. pmc Effects of phosphorylation on phosphoenolpyruvate carboxykinase from the C4 plant Guinea grass
    Robert P Walker
    Robert Hill Institute and Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, United Kingdom
    Plant Physiol 128:165-72. 2002
    In the C4 plant Guinea grass (Panicum maximum), phosphoenolpyruvate carboxykinase (PEPCK) is phosphorylated in darkened leaves and dephosphorylated in illuminated leaves...
  7. ncbi Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus
    Alejandro F Alice
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
    Microbiology 149:1687-98. 2003
    ..Both HPr and EI proteins were fully functional for phosphoenolpyruvate-dependent transphosphorylation in complementation assays using extracts from Staphylococcus aureus mutants ..
  8. pmc Phosphoenolpyruvate carboxykinase as the sole anaplerotic enzyme in Saccharomyces cerevisiae
    Rintze M Zelle
    Department of Biotechnology, Delft University of Technology and Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, Delft, Netherlands
    Appl Environ Microbiol 76:5383-9. 2010
    ..In several succinate-producing prokaryotes, phosphoenolpyruvate carboxykinase (PEPCK) fulfills this anaplerotic role. However, the S...
  9. ncbi Cloning and sequencing of two enterococcal glpK genes and regulation of the encoded glycerol kinases by phosphoenolpyruvate-dependent, phosphotransferase system-catalyzed phosphorylation of a single histidyl residue
    V Charrier
    Institut de Biologie et Chimie des Proteines, CNRS, 7 passage du Vercors, F 69367 Lyon Cedex 07, France
    J Biol Chem 272:14166-74. 1997
    ..were purified and were found to be phosphorylated by enzyme I and the histidine-containing protein of the phosphoenolpyruvate:glycose phosphotransferase system...
  10. ncbi Phosphoenolpyruvate, a glycolytic intermediate, as a cytoprotectant and antioxidant in ex-vivo cold-preserved mouse liver: a potential application for organ preservation
    Yuki Kondo
    Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Pharm Pharmacol 65:390-401. 2013
    The aim of this study was to examine the effect of phosphoenolpyruvate (PEP), a glycolytic intermediate, on organ damage during cold preservation of liver.
  11. ncbi The high-resolution structure of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase reveals a twist in the plane of bound phosphoenolpyruvate
    Igor A Shumilin
    Department of Biology, University of Virginia, Charlottesville, Virginia 22903 2477, USA
    Biochemistry 42:3766-76. 2003
    ..of the aromatic biosynthetic pathway in microorganisms and plants, catalyzes the aldol-like condensation of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) with the formation of DAHP...
  12. ncbi Phosphoenolpyruvate- and ATP-dependent dihydroxyacetone kinases: covalent substrate-binding and kinetic mechanism
    Luis F Garcia-Alles
    Department of Chemistry and Biochemistry and Laboratory for Chemical and Mineralogical Crystallography, University of Berne, CH 3012 Berne, Switzerland
    Biochemistry 43:13037-45. 2004
    ..two different phosphoryldonors, ATP in animals, plants, and some bacteria, and a multiphosphoprotein of the phosphoenolpyruvate carbohydrate phosphotransferase system (PTS) in most bacteria...
  13. ncbi Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS)
    C Siebold
    , , CH-3012, Bern, Switzerland
    FEBS Lett 504:104-11. 2001
    ..Purified, cyclized IIA(Glc) and IICB(Glc) had 100% and 30% of wild-type glucose phosphotransferase activity, respectively. Cyclized IIA(Glc) displayed increased stability against temperature and GuHCl-induced unfolding...
  14. ncbi Phosphoenolpyruvate-dependent inhibition of collagen biosynthesis, alpha2beta1 integrin and IGF-I receptor signaling in cultured fibroblasts
    Ewa Karna
    Department of Medicinal Chemistry, Medical University in Bialystok, Kilinskiego 1, Bialystok, Poland
    Mol Cell Biochem 315:61-7. 2008
    ..The finding that prolidase plays an important role in collagen biosynthesis and phosphoenolpyruvate inhibits prolidase activity "in vitro" led to evaluate its effect on collagen biosynthesis in cultured ..
  15. ncbi Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase of Thermoproteus tenax: key pieces in the puzzle of archaeal carbohydrate metabolism
    Britta Tjaden
    Department of Microbiology, Universitat Duisburg Essen, 45117 Essen, Germany
    Mol Microbiol 60:287-98. 2006
    The interconversion of phosphoenolpyruvate and pyruvate represents an important control point of the Embden-Meyerhof-Parnas (EMP) pathway in Bacteria and Eucarya, but little is known about this site of regulation in Archaea...
  16. ncbi Arginine residues at the active site of avian liver phosphoenolpyruvate carboxykinase
    K C Cheng
    Department of Chemistry, University of Notre Dame, Indiana 46556
    J Biol Chem 264:3317-24. 1989
    The presence of arginine at the active site of avian liver phosphoenolpyruvate carboxykinase was studied by chemical modification followed by a characterization of the modified enzyme...
  17. ncbi Purification and properties of phosphoenolpyruvate carboxylase from Molinema dessetae (Nematoda: Filarioidea)
    P M Loiseau
    Biologie et Contrôle des Organismes Parasites, Universite de Paris Sud, Chatenay Malabry
    Parasitology 106:55-61. 1993
    The presence of phosphoenolpyruvate (PEP) carboxylase (EC 4.1.1.31), an enzyme at the branchpoint of glycolysis and the Krebs cycle was detected in the Filaria Molinema dessetae...
  18. ncbi Inhibition of mitochondrial respiration by phosphoenolpyruvate
    C Nathan Baily
    Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, USA
    Arch Biochem Biophys 514:68-74. 2011
    ..In this report, we examine the effects of phosphoenolpyruvate (PEP), a glycolytic intermediate, on mitochondrial function...
  19. pmc First characterization of an archaeal GTP-dependent phosphoenolpyruvate carboxykinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1
    Wakao Fukuda
    Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo ku, Kyoto 615 8510, Japan
    J Bacteriol 186:4620-7. 2004
    b>Phosphoenolpyruvate carboxykinase (PCK), which catalyzes the nucleotide-dependent, reversible decarboxylation of oxaloacetate to yield phosphoenolpyruvate and CO2, is one of the important enzymes in the interconversion between C3 and C4 ..
  20. pmc Experimental evidence of phosphoenolpyruvate resynthesis from pyruvate in illuminated leaves
    Guillaume Tcherkez
    Institut de Biologie des Plantes, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8618, France
    Plant Physiol 157:86-95. 2011
    ..acid synthesis, but pyruvate metabolism is not well documented, and neither is the possible resynthesis of phosphoenolpyruvate (PEP)...
  21. ncbi Peptides as inhibitors of the first phosphorylation step of the Streptomyces coelicolor phosphoenolpyruvate: sugar phosphotransferase system
    Rosa Doménech
    Instituto de Biologia Molecular y Celular, Universidad Miguel Hernandez, Elche Alicante, Spain
    Biochemistry 51:7393-402. 2012
    ..Because the affinity of intact EIN(sc) for the whole HPr(sc) is 12 μM, we suggest that the assayed peptides might be considered as good hit compounds for inhibiting the interaction between HPr(sc) and EIN(sc)...
  22. ncbi The PEP-pyruvate-oxaloacetate node as the switch point for carbon flux distribution in bacteria
    Uwe Sauer
    Institute of Biotechnology, ETH Zurich, Switzerland
    FEMS Microbiol Rev 29:765-94. 2005
    In many organisms, metabolite interconversion at the phosphoenolpyruvate (PEP)-pyruvate-oxaloacetate node involves a structurally entangled set of reactions that interconnects the major pathways of carbon metabolism and thus, is ..
  23. ncbi Streptomyces olivaceoviridis possesses a phosphotransferase system that mediates specific, phosphoenolpyruvate-dependent uptake of N-acetylglucosamine
    F Wang
    Fachbereich Biologie chemie, Universitat Osnabruck, Barbarastr 11, 49069 Osnabruck, Germany
    Mol Genet Genomics 268:344-51. 2002
    ..This phenotype can now be attributed to a functional phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS)...
  24. pmc Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla 92093 0116, USA
    J Bacteriol 178:5480-6. 1996
    ..We here demonstrate that mutation of His-15 of HPr, the site of phosphoenolpyruvate-dependent phosphorylation, also prevents carbon catabolite repression of the gnt operon...
  25. pmc Phosphoenolpyruvate provision to plastids is essential for gametophyte and sporophyte development in Arabidopsis thaliana
    Veena Prabhakar
    Universitat zu Koln, Biozentrum, Botanisches Institut II, D 50674 Cologne, Germany
    Plant Cell 22:2594-617. 2010
    Restriction of phosphoenolpyruvate (PEP) supply to plastids causes lethality of female and male gametophytes in Arabidopsis thaliana defective in both a phosphoenolpyruvate/phosphate translocator (PPT) of the inner envelope membrane and ..
  26. ncbi Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range
    Zhili Li
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    Anal Biochem 343:35-47. 2005
    Escherichia coli 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of phosphoenolpyruvate (PEP) and D-arabinose 5-phosphate (A5P) to produce KDO8P and inorganic phosphate...
  27. ncbi Inhibitory effect of phosphoenolpyruvate on glycolytic enzymes in Escherichia coli
    Tadashi Ogawa
    Department of Biochemistry, Aichi Medical University School of Medicine, Nagakute, Aichi 480 1195, Japan
    Res Microbiol 158:159-63. 2007
    ..coli K12 W3110 that were constructed with the vector pCA24N. Phosphoenolpyruvate (PEP) acted as an effective inhibitor of enzymes of the preparatory phase in glycolysis...
  28. ncbi Spatial stochastic modelling of the phosphoenolpyruvate-dependent phosphotransferase (PTS) pathway in Escherichia coli
    J Vidal Rodríguez
    Section Computational Science, Faculty of Science, University of Amsterdam Kruislaan 403, 1098 SJ Amsterdam, The Netherlands
    Bioinformatics 22:1895-901. 2006
    ..Pathways usually consist of a complex interaction network and are characterized by a large set of parameters. The inclusion of spatial and stochastic effects is a major challenge in developing quantitative and dynamic models of pathways...
  29. pmc Analysis of global control of Escherichia coli carbohydrate uptake
    Andreas Kremling
    Max Planck Institut Magdeburg, Systems Biology, Sandtorstr, 1, 39106 Magdeburg, Germany
    BMC Syst Biol 1:42. 2007
    ..A detailed understanding of the coordination of the control of individual transporters offers possibilities to explore the potential of microorganisms e.g. in biotechnology...
  30. pmc Opposing effects of phosphoenolpyruvate and pyruvate with Mg(2+) on the conformational stability and dimerization of phosphotransferase enzyme I from Escherichia coli
    Mariana N Dimitrova
    Section on Protein Chemistry, Laboratory of Biochemistry, National Heart, Lung, and Blood Institute NHLBI, National Institutes of Health NIH, Bethesda, Maryland 20892 8012, USA
    Protein Sci 12:2047-56. 2003
    ..The binding of PEP to EI(H189A) is synergistic with that of Mg(2+). Thus, physiological concentrations of PEP and Mg(2+) increase, whereas pyruvate and Mg(2+) decrease the amount of dimeric, active, dephospho-enzyme I...
  31. pmc Disentangling the web of allosteric communication in a homotetramer: heterotropic inhibition in phosphofructokinase from Escherichia coli
    Aron W Fenton
    Department of Biochemistry and Biophysics, Texas A and M University and Texas AgriLife Research, College Station, Texas 77843 2128, USA
    Biochemistry 48:12323-8. 2009
    ..of each of the four unique inhibiting heterotropic interactions between the allosteric inhibitor, phosphoenolpyruvate (PEP), and the substrate, fructose 6-phosphate (Fru-6-P), in phosphofructokinase from Escherichia coli (..
  32. ncbi Metabolic flux analysis of pykF gene knockout Escherichia coli based on 13C-labeling experiments together with measurements of enzyme activities and intracellular metabolite concentrations
    K Al Zaid Siddiquee
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, 820 8502 Fukuoka, Japan
    Appl Microbiol Biotechnol 63:407-17. 2004
    ..To clarify the metabolism quantitatively, it was found to be quite important to integrate the information on intracellular metabolic flux distribution, enzyme activities and intracellular metabolite concentrations...
  33. ncbi Differential regulation of endogenous glucose-6-phosphatase and phosphoenolpyruvate carboxykinase gene expression by the forkhead transcription factor FKHR in H4IIE-hepatoma cells
    A Barthel
    Institut fur Pharmakologie und Toxikologie, RWTH Aachen, Aachen, D 52057, Germany
    Biochem Biophys Res Commun 285:897-902. 2001
    ..H4 cells) was used in order to determine the role of the transcription factor FKHR in the regulation of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase)...
  34. ncbi Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution
    E Zhang
    Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA
    Biochemistry 36:12526-34. 1997
    Enolase, a glycolytic enzyme that catalyzes the dehydration of 2-phospho-d-glycerate (PGA) to form phosphoenolpyruvate (PEP), is a homodimer in all eukaryotes and many prokaryotes...
  35. ncbi 3-Deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli. Model of binding of phosphoenolpyruvate and D-arabinose-5-phosphate
    T Wagner
    Department of Biology, University of Virginia, Charlottesville, VA, 22903 2477, USA
    J Mol Biol 301:233-8. 2000
    ..coli KDOPS, grown in 24 % (w/v) polyethylene glycol (PEG) 1500 in the presence of the substrates, 2-phosphoenolpyruvate (PEP) and d-arabinose-5-phosphate (A5P), are also in space group I23 (a=118...
  36. ncbi Molecular analysis of the glucose-specific phosphoenolpyruvate : sugar phosphotransferase system from Lactobacillus casei and its links with the control of sugar metabolism
    María J Yebra
    Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, Apdo Correos 73, 46100 Burjassot, Spain
    Microbiology 152:95-104. 2006
    Lactobacillus casei transports glucose preferentially by a mannose-class phosphoenolpyruvate : sugar phosphotransferase system (PTS). The genomic analysis of L...
  37. ncbi Transient state kinetics of Enzyme I of the phosphoenolpyruvate:glycose phosphotransferase system of Escherichia coli: equilibrium and second-order rate constants for the phosphotransfer reactions with phosphoenolpyruvate and HPr
    Norman D Meadow
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 44:12790-6. 2005
    The first two reactions in the phosphotransfer sequence of bacterial phosphoenolpyruvate:glycose phosphotransferase systems are the autophosphorylation of Enzyme I by phosphoenolpyruvate followed by the transfer of the phospho group to ..
  38. pmc Structure of phosphorylated enzyme I, the phosphoenolpyruvate:sugar phosphotransferase system sugar translocation signal protein
    Alexey Teplyakov
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 103:16218-23. 2006
    Bacterial transport of many sugars, coupled to their phosphorylation, is carried out by the phosphoenolpyruvate (PEP):sugar phosphotransferase system and involves five phosphoryl group transfer reactions...
  39. ncbi Molecular characterization of a phosphoenolpyruvate carboxylase from a thermophilic cyanobacterium, Synechococcus vulcanus with unusual allosteric properties
    Li Mei Chen
    Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto, 606 8502 Japan
    Plant Cell Physiol 43:159-69. 2002
    A gene for phosphoenolpyruvate carboxylase (PEPC) was isolated from a thermophilic cyanobacterium, Synechococcus vulcanus, by screening a genomic DNA library using the coding region of Anacystis nidulans 6301 PEPC as a probe. The S...
  40. pmc A new class of plastidic phosphate translocators: a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter
    K Fischer
    Botanisches Institut, Universitat zu Koln, Germany
    Plant Cell 9:453-62. 1997
    ..transport of inorganic phosphate in exchange with C3 compounds phosphorylated at C-atom 2, particularly phosphoenolpyruvate, which is required inside the plastids for the synthesis of, for example, aromatic amino acids...
  41. pmc Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose limitation in Escherichia coli
    Annik Nanchen
    Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
    J Bacteriol 190:2323-30. 2008
    ..most relevant control mechanism was cyclic AMP (cAMP)-dependent catabolite repression as the inducer of the phosphoenolpyruvate (PEP)-glyoxylate cycle and thus low tricarboxylic acid cycle fluxes...
  42. ncbi Inducer exclusion in Escherichia coli by non-PTS substrates: the role of the PEP to pyruvate ratio in determining the phosphorylation state of enzyme IIAGlc
    B M Hogema
    E C Slater Institute, BioCentrum, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
    Mol Microbiol 30:487-98. 1998
    ..repression in Escherichia coli is the dephosphorylation of enzyme IIAGlc, one of the enzymes of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS)...
  43. ncbi Pathway engineering for the production of aromatic compounds in Escherichia coli
    N Flores
    Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
    Nat Biotechnol 14:620-3. 1996
    ..formation of glucose-6-phosphate through the glucose phosphotransferase system (PTS), one molecule of phosphoenolpyruvate (PEP) is consumed...
  44. ncbi A global regulatory role of gluconeogenic genes in Escherichia coli revealed by transcriptome network analysis
    Katy C Kao
    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, USA
    J Biol Chem 280:36079-87. 2005
    ..These results suggest that the gluconeogenic flux to phosphoenolpyruvate is important for full activation of adenylate cyclase through the phosphorylated enzyme IIA(glu) of the ..
  45. ncbi pH Dependence of the reaction catalyzed by avian mitochondrial phosphoenolpyruvate carboxykinase
    Todd Holyoak
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Biochemistry 43:7054-65. 2004
    The pH dependence of the reaction catalyzed by phosphoenolpyruvate carboxykinase (PEPCK) provides significant insight into the chemical mechanism...
  46. ncbi Lack of glyconeogenesis in pancreatic islets: expression of gluconeogenic enzyme genes in islets
    M J MacDonald
    Childrens Diabetes Center, University of Wisconsin Medical School, Madison
    Horm Metab Res 24:158-60. 1992
    ..pyruvate carboxylase mRNA, but even islets from rats which had been starved (a condition which induces phosphoenolpyruvate carboxykinase (PEPCK) in liver, kidney and adipose tissue) showed no PEPCK mRNA...
  47. ncbi Nutrient-scavenging stress response in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system, as explored by gene expression profile analysis
    Salvador Flores
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mexico
    J Mol Microbiol Biotechnol 10:51-63. 2005
    The physiological role of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) has been studied in Escherichia coli...
  48. ncbi The import of phosphoenolpyruvate by plastids from developing embryos of oilseed rape, Brassica napus (L.), and its potential as a substrate for fatty acid synthesis
    Sybille E Kubis
    Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
    J Exp Bot 55:1455-62. 2004
    The plastidial phosphoenolpyruvate (PEP)/phosphate translocator (PPT) is expressed in the developing embryos of oilseed rape (Brassica napus L.)...
  49. pmc Glucose transport by a mutant of Streptococcus mutans unable to accumulate sugars via the phosphoenolpyruvate phosphotransferase system
    D G Cvitkovitch
    Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada
    J Bacteriol 177:2251-8. 1995
    Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS)...
  50. ncbi Structural and mechanistic analysis of sialic acid synthase NeuB from Neisseria meningitidis in complex with Mn2+, phosphoenolpyruvate, and N-acetylmannosaminitol
    Jason Gunawan
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3
    J Biol Chem 280:3555-63. 2005
    ..NeuB catalyzes the condensation of phosphoenolpyruvate (PEP) and N-acetylmannosamine, directly forming N-acetylneuraminic acid (or sialic acid)...
  51. pmc Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coli
    Annik Nanchen
    Institute of Molecular Systems Biology, ETH Zurich, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 72:1164-72. 2006
    ..this glyoxylate shunt activity is best understood from a network perspective as the recently described phosphoenolpyruvate (PEP)-glyoxylate cycle that oxidizes PEP (or pyruvate) to CO2...
  52. pmc The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression
    S J Streatfield
    Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Plant Cell 11:1609-22. 1999
    ..In this study, we determine that CUE1 encodes the plastid inner envelope phosphoenolpyruvate/phosphate translocator (PPT) and define amino acid residues that are critical for translocator function...
  53. ncbi Phosphoenolpyruvate-dependent tubulin-pyruvate kinase interaction at different organizational levels
    Janos Kovacs
    Department of General Zoology, Eotvos Lorand University, P O Box 330, H 1445 Budapest, Hungary
    J Biol Chem 278:7126-30. 2003
    Evidence for the direct binding of pyruvate kinase to tubulin/microtubule and for the inhibitory effect of phosphoenolpyruvate on tubulin-enzyme hetero-association were provided by surface plasmon resonance and pelleting experiments...
  54. ncbi Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site
    Pete Dunten
    Roche Research Center, Hoffmann La Roche Inc, Nutley, NJ 07110, USA
    J Mol Biol 316:257-64. 2002
    We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates...
  55. ncbi The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA
    Rosa Viana
    Biotechnology Department, Instituto de Agroquimica y Tecnologia de Alimentos, IATA CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
    Arch Microbiol 183:385-93. 2005
    ..casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L...
  56. pmc Fructose degradation in the haloarchaeon Haloferax volcanii involves a bacterial type phosphoenolpyruvate-dependent phosphotransferase system, fructose-1-phosphate kinase, and class II fructose-1,6-bisphosphate aldolase
    Andreas Pickl
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat Kiel, Am Botanischen Garten, Kiel, Germany
    J Bacteriol 194:3088-97. 2012
    ..This is the first report of the functional involvement of a bacterial-like PTS and of class II FBA in the sugar metabolism of archaea...
  57. ncbi Acetate metabolism in Escherichia coli strains lacking phosphoenolpyruvate: carbohydrate phosphotransferase system; evidence of carbon recycling strategies and futile cycles
    Juan Carlos Sigala
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca Morelos, Mexico
    J Mol Microbiol Biotechnol 16:224-35. 2009
    ..recovered its growth capacity on glucose by an adaptive evolution process (PB12, PTS(-)Glc(+)), part of the phosphoenolpyruvate not used in glucose transport has been utilized for the synthesis of aromatic compounds...
  58. ncbi A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli
    Eliane Fischer
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    J Biol Chem 278:46446-51. 2003
    ....
  59. ncbi The quaternary structure of pyruvate kinase type 1 from Escherichia coli at low nanomolar concentrations
    Tong Zhu
    University of Canterbury, Christchurch, New Zealand
    Biochimie 92:116-20. 2010
    ..In addition, we show that, unlike some other PK isoenzymes, the modulation of oligomeric state by the allosteric effectors FBP and PEP does not occur at a concentration of 10 nM or above...
  60. pmc Phosphoenolpyruvate cycling via mitochondrial phosphoenolpyruvate carboxykinase links anaplerosis and mitochondrial GTP with insulin secretion
    Romana Stark
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 284:26578-90. 2009
    ..Here we have investigated whether the mitochondrial isoform of phosphoenolpyruvate carboxykinase (PEPCK-M) is the GTPase linking hydrolysis of mtGTP made by succinyl-CoA synthetase (SCS-GTP) ..
  61. ncbi Essential cysteines in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from Escherichia coli: analysis by chemical modification and site-directed mutagenesis
    H M Salleh
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    Biochemistry 35:8942-7. 1996
    ..1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was ..
  62. ncbi Cloning and in vivo expression of functional triose phosphate/phosphate translocators from C3- and C4-plants: evidence for the putative participation of specific amino acid residues in the recognition of phosphoenolpyruvate
    K Fischer
    Julius von Sachs Institut für Biowissenschaften der Universität Würzburg, Germany
    Plant J 5:215-26. 1994
    ..between the recombinant translocator proteins from the two cell types with respect to the transport of phosphoenolpyruvate. Comparison of the cTPT sequences from F. pringlei and F...
  63. ncbi Structure of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli: comparison of the Mn(2+)*2-phosphoglycolate and the Pb(2+)*2-phosphoenolpyruvate complexes and implications for catalysis
    T Wagner
    Department of Biology, University of Virginia, Charlottesville, VA 22903 2477, USA
    J Mol Biol 301:389-99. 2000
    ..distorted octahedral coordination sphere of the metal is completed by PGL, which replaces the substrate, 2-phosphoenolpyruvate (PEP), in the active site...
  64. ncbi The use of (E)- and (Z)-phosphoenol-3-fluoropyruvate as mechanistic probes reveals significant differences between the active sites of KDO8P and DAHP synthases
    Cristina M Furdui
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
    Biochemistry 44:7326-35. 2005
    ..DAHP) synthase catalyze a similar aldol-type condensation between phosphoenolpyruvate (PEP) and the corresponding aldose: arabinose 5-phosphate (A5P) and erythrose 4-phosphate (E4P), ..
  65. ncbi Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes
    Luis Fernando Garcia-Alles
    Departement fur Chemie und Biochemie, Universitat Bern, Freiestrasse 3, CH 3012 Bern, Switzerland
    Eur J Biochem 269:3226-36. 2002
    The synthesis of 10 new phosphoenolpyruvate (PEP) analogues with modifications in the phosphate and the carboxylate function is described. Included are two potential irreversible inhibitors of PEP-utilizing enzymes...
  66. pmc Stoichiometry and substrate affinity of the mannitol transporter, EnzymeIImtl, from Escherichia coli
    Gertjan Veldhuis
    Department of Biochemistry and Biophysical Chemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands
    Biophys J 89:201-10. 2005
    ..The dependency could be simulated assuming that >50% of the binding sites were occupied with a ligand that shows an affinity for EnzymeIImtl in the same range as mannitol...
  67. pmc Comparative analysis of the Escherichia coli ketopantoate hydroxymethyltransferase crystal structure confirms that it is a member of the (betaalpha)8 phosphoenolpyruvate/pyruvate superfamily
    Florian Schmitzberger
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    J Bacteriol 185:4163-71. 2003
    ..We show that KPHMT forms a family within the phosphoenolpyruvate/pyruvate superfamily...
  68. ncbi Molecular and regulatory properties of leucoplast pyruvate kinase from Brassica napus (rapeseed) suspension cells
    William C Plaxton
    Department of Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Arch Biochem Biophys 400:54-62. 2002
    ..from Brassica napus suspension cells was purified 431-fold to a final specific activity of 28 micromol phosphoenolpyruvate (PEP) utilized/min/mg protein...
  69. ncbi The monomer/dimer transition of enzyme I of the Escherichia coli phosphotransferase system
    Himatkumar V Patel
    Department of Biology, The Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    J Biol Chem 281:17570-8. 2006
    Enzyme I (EI) is the first protein in the phosphotransfer sequence of the bacterial phosphoenolpyruvate:glycose phosphotransferase system. This system catalyzes sugar phosphorylation/transport and is stringently regulated...
  70. ncbi Structural and mechanistic changes along an engineered path from metallo to nonmetallo 3-deoxy-D-manno-octulosonate 8-phosphate synthases
    Fathima Kona
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 46:4532-44. 2007
    ..These differences may reflect an evolutionary adaptation of metallo and nonmetallo KDO8PS's to the cellular concentrations of these metabolites in their respective hosts...
  71. ncbi Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis
    J Wang
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 40:15676-83. 2001
    ..3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase from Aquifex aeolicus in complex with phosphoenolpyruvate (PEP) and ribose 5-phosphate (R5P), and with a bisubstrate inhibitor that mimics the postulated linear ..
  72. ncbi Human erythrocyte pyruvate kinase: characterization of the recombinant enzyme and a mutant form (R510Q) causing nonspherocytic hemolytic anemia
    C Wang
    Department of Biochemistry, University of Pavia, Pavia, Italy
    Blood 98:3113-20. 2001
    ..properties characteristic of authentic human erythrocyte pyruvate kinase, including response to substrate, phosphoenolpyruvate, activation by fructose 1,6-bisphosphate, and inhibition by adenosine triphosphate (ATP)...
  73. ncbi Kinetic linked-function analysis of the multiligand interactions on Mg(2+)-activated yeast pyruvate kinase
    T J Bollenbach
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Biochemistry 40:13097-106. 2001
    ..These results lend support to the hypothesis that divalent metals modulate the interactions of ligands on YPK and that divalent metals play a role in regulation of the glycolytic pathway...
  74. ncbi Influence of inorganic pyrophosphate on the kinetics of muscle pyruvate kinase: a simple nonallosteric feedback model
    Marian Kuczek
    Department of Experimental and Applied Biology, University of Opole, Kominka 4, Poland
    Biosystems 66:11-20. 2002
    ..According to this model, mutual and simultaneous interactions of reaction products with substrates and with an enzyme result in the coexistence of simple and mixed, labile and inert complexes...
  75. ncbi Enzyme I of the phosphotransferase system: induced-fit protonation of the reaction transition state by Cys-502
    Luis Fernando Garcia-Alles
    Departement fur Chemie und Biochemie, Universitat Bern, Switzerland
    Biochemistry 42:4744-50. 2003
    Enzyme I (EI), the first component of the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS), consists of an N-terminal domain with the phosphorylation site (His-189) and a C-terminal domain with the PEP binding site...
  76. ncbi Substrate and metal complexes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae provide new insights into the catalytic mechanism
    Verena König
    Department of Structural Chemistry, University of Gottingen, Tammannstr 4, D 37077 Gottingen, Germany
    J Mol Biol 337:675-90. 2004
    ....
  77. ncbi Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus
    Linley R Schofield
    Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand
    Protein Expr Purif 34:17-27. 2004
    ..3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAH7PS) catalyzes the condensation reaction between phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P)...
  78. ncbi Structure of the full-length enzyme I of the phosphoenolpyruvate-dependent sugar phosphotransferase system
    Joséa Márquez
    European Molecular Biology Laboratory, Structural and Computational Biology Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 281:32508-15. 2006
    Enzyme I (EI) is the phosphoenolpyruvate (PEP)-protein phosphotransferase at the entry point of the PEP-dependent sugar phosphotransferase system, which catalyzes carbohydrate uptake into bacterial cells...
  79. ncbi Identification of the catalytic residues of AroA (Enolpyruvylshikimate 3-phosphate synthase) using partitioning analysis
    Shehadeh Mizyed
    Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4M1, Canada
    Biochemistry 42:6986-95. 2003
    AroA (EPSP synthase) catalyzes carboxyvinyl transfer through addition of shikimate 3-phosphate (S3P) to phosphoenolpyruvate (PEP) to form a tetrahedral intermediate (THI), followed by phosphate elimination to give enolpyruvylshikimate 3-..
  80. ncbi Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily
    Celia C H Chen
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Biochemistry 45:11491-504. 2006
    Phosphonopyruvate (P-pyr) hydrolase (PPH), a member of the phosphoenolpyruvate (PEP) mutase/isocitrate lyase (PEPM/ICL) superfamily, hydrolyzes P-pyr and shares the highest sequence identity and functional similarity with PEPM...
  81. ncbi Pyruvate kinase: current status of regulatory and functional properties
    Ma Enriqueta Muñoz
    Facultad de Ciencias Quíicas e Ingenierí, Universidad Autonoma de Baja California, Av Tecnologico s n, Mesa de Otay, B C, Tijuana, Mexico C P 22390
    Comp Biochem Physiol B Biochem Mol Biol 135:197-218. 2003
    ..coli, cat and rabbit muscle. A number of attributes of the enzyme that make it of particular interest in biomedicine and industry are also discussed...
  82. ncbi Metabolic flux redistribution in Corynebacterium glutamicum in response to osmotic stress
    C Varela
    Departamento de Ingeniería Química y Bioprocesos, Pontificia Universidad Catolica de Chile, Casilla 306 Correo 22, Santiago, Chile
    Appl Microbiol Biotechnol 60:547-55. 2003
    ..glutamicum...
  83. ncbi The effect of active site mutations in the oxaloacetate decarboxylase and pyruvate kinase-like activities of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase
    Ana María Jabalquinto
    Departamento de Ciencias Quimicas, Facultad de Quimica y Biologia, Universidad de Santiago de Chile, Casilla 40, Santiago 33, Chile
    J Protein Chem 21:443-5. 2002
    Anaerobiospirillum succiniciproducens His225Gln, Asp262Asn, Asp263Asn, and Thr249Asn phosphoenolpyruvate carboxykinases were analyzed for their oxaloacetate decarboxylase, and pyruvate kinase-like activities...
  84. ncbi Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system
    Himatkumar V Patel
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    J Biol Chem 281:17579-87. 2006
    The bacterial phosphoenolpyruvate (PEP):glycose phosphotransferase system (PTS) mediates uptake/phosphorylation of sugars. The transport of all PTS sugars requires Enzyme I (EI) and a phosphocarrier histidine protein of the PTS (HPr)...
  85. ncbi Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase
    Jian Wang
    Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Mol Biol 324:205-14. 2002
    ..8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) by favoring the activation of a water molecule coordinated to the active-site metal ion...
  86. ncbi Detection of novel enzyme intermediates in PEP-utilizing enzymes
    Karen S Anderson
    Department of Pharmacology, SHM B350B, Yale University School of Medicine, 333 Cedar Street New Haven, CT 06520, USA
    Arch Biochem Biophys 433:47-58. 2005
    ..A novel application of this approach using time-resolved electrospray mass spectrometry to detect chemically labile enzyme intermediates is also discussed...
  87. ncbi How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli
    Susanne Eschenburg
    Max Planck Institute for Medical Research, Department of Biophysics, 69120 Heidelberg, Germany
    Planta 216:129-35. 2002
    ..substrate, shikimate-3-phosphate (S3P), but displays a 30-fold lower affinity for its second substrate, phosphoenolpyruvate (PEP)...
  88. ncbi New bioreactor-coupled rapid stopped-flow sampling technique for measurements of metabolite dynamics on a subsecond time scale
    Stefan Buziol
    Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, D 70569 Stuttgart, Germany
    Biotechnol Bioeng 80:632-6. 2002
    ....
  89. ncbi Modification of human erythrocyte pyruvate kinase by an active site-directed reagent: bromopyruvate
    N L Acan
    Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey
    J Enzyme Inhib 16:457-64. 2001
    ..When the enzyme was modified with bromopyruvate in the absence of adenosine-5'-diphosphate, phosphoenolpyruvate or fructose-1,6-diphosphate the inactivation followed a pseudo first-order kinetics...
  90. ncbi Phosphoenolpyruvate metabolism in Jerusalem artichoke mitochondria
    Lidia de Bari
    Istituto di Biomembrane e Bioenergetica, CNR, Via G Amendola 165 A, 70126, Bari, Italy
    Biochim Biophys Acta 1767:281-94. 2007
    We report here initial studies on phosphoenolpyruvate metabolism in coupled mitochondria isolated from Jerusalem artichoke tubers...
  91. ncbi S-phosphocysteine and phosphohistidine are intermediates in the phosphoenolpyruvate-dependent mannitol transport catalyzed by Escherichia coli EIIMtl
    H H Pas
    Department of Physical Chemistry, University of Groningen, The Netherlands
    Biochemistry 27:5835-9. 1988
    ..The hydrolysis characteristics of the phosphopeptide indicate that Cys 384 is the site of phosphorylation...
  92. ncbi Catalytic role of an arginine residue in the highly conserved and unique sequence of phosphoenolpyruvate carboxylase
    M Yano
    Faculty of Science, Kyoto University
    J Biochem 117:1196-200. 1995
    b>Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues...
  93. ncbi Detection and characterization of a phospholactoyl-enzyme adduct in the reaction catalyzed by UDP-N-acetylglucosamine enolpyruvoyl transferase, MurZ
    E D Brown
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 33:10638-45. 1994
    The MurZ enzyme catalyzes enolpyruvoyl transfer from phosphoenolpyruvate to the 3-OH of UDP-N-acetylglucosamine (UDP-GlcNAc) in Escherichia coli peptidoglycan biosynthesis...
  94. ncbi Proton donor in yeast pyruvate kinase: chemical and kinetic properties of the active site Thr 298 to Cys mutant
    Delia Susan-Resiga
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Biochemistry 43:15230-45. 2004
    ..The results are consistent with water being the active site proton donor. Active site residue T298 is not critical for activity but plays a role in the activation of the water and affects the pK that modulates catalytic activity...
  95. ncbi ADP modulates the dynamic behavior of the glycolytic pathway of Escherichia coli
    J C Diaz Ricci
    Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas CONICET UNT, Instituto de Química Biológica Dr Bernabe Bloj, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Argentina
    Biochem Biophys Res Commun 271:244-9. 2000
    ..The influence of ADP, phosphoenolpyruvate (PEP), and fructose-6-phosphate (F6P) on the dynamic regulation of this pathway is also analyzed...
  96. ncbi Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3-phosphate synthase and its activation by univalent cations
    W Du
    Anti infectives Research, SmithKline Beecham Pharmaceuticals, Collegeville, PA 19426, USA
    Eur J Biochem 267:222-7. 2000
    ..It was shown to catalyze a reversible conversion of shikimate 3-phosphate (S3P) and phosphoenolpyruvate (PEP) to EPSP and inorganic phosphate...
  97. ncbi Cysteine phosphorylation of the glucose transporter of Escherichia coli
    M Meins
    Department of Microbiology, University of Basel, Switzerland
    J Biol Chem 268:11604-9. 1993
    ..Phosphocysteine has been shown previously to be the catalytic intermediate of the mannitol transporter (Pas, H. H., Meyer, G. H., Kruizinga, W. H., Tamminga, K. S., van Weeghel, R. P., and Robillard, G. T...
  98. ncbi Plausible phosphoenolpyruvate binding site revealed by 2.6 A structure of Mn2+-bound phosphoenolpyruvate carboxylase from Escherichia coli
    H Matsumura
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Japan
    FEBS Lett 458:93-6. 1999
    We have determined the crystal structure of Mn2+-bound Escherichia coli phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2...
  99. ncbi The catalytic mechanism of EPSP synthase revisited
    J Lewis
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520 8066, USA
    Biochemistry 38:7372-9. 1999
    ....
  100. ncbi Catalytic mechanism of Kdo8P synthase: transient kinetic studies and evaluation of a putative reaction intermediate
    P H Liang
    Department of Chemistry, Technion Israel Institute of Technology, Haifa, Israel
    Biochemistry 37:16390-9. 1998
    ..It was found however that 2 binds to the enzyme and is a competitive inhibitor with respect to phosphoenolpyruvate binding, having a Ki value of 35 microM...
  101. ncbi A tetrahedral intermediate in the EPSP synthase reaction observed by rapid quench kinetics
    K S Anderson
    Monsanto Agricultural Company, A Unit of Monsanto Company, St Louis, Missouri 63167
    Biochemistry 27:7395-406. 1988
    ..3, which is largely a measure of K4.(ABSTRACT TRUNCATED AT 250 WORDS)..

Research Grants62

  1. PUFA Synthesis and the Control of Hepatic Metabolism
    Donald B Jump; Fiscal Year: 2013
    ..g., phosphoenolpyruvate carboxykinase and glucose-6 phosphatase...
  2. Atypical PKC Knockout Models: Effect on Glucose and Lipid Homeostasis
    Robert V Farese; Fiscal Year: 2013
    ..in hepatic insulin sensitivity and suppression of hepatic glucose output, owing to decreased expression of phosphoenolpyruvate carboxykinase (PEPCK)...
  3. Cellular Mechanisms for Increased Gluconeogenesis in Type 2 Diabetes
    Varman T Samuel; Fiscal Year: 2013
    ..Many ascribed increased gluconeogenesis to increased transcription of phosphoenolpyruvate carboxykinase (PEPCK). However, our recent findings challenge this dogma...
  4. Activation of Atypical PKC by Insulin and Other Agonists
    ROBERT VITO FARESE; Fiscal Year: 2013
    ..as an added benefit, inhibition of hepatic aPKC surprisingly diminishes expression of glucogenic enzymes, phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase)...
  5. Notch1-FoxO1 interaction in regulation of hepatic gluconeogenesis
    UTPAL BHAGIRATH PAJVANI; Fiscal Year: 2010
    ..transcription of the rate-limiting enzymes in glucose production, glucose-6 phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK)...
  6. Small RNAs and Vibrio cholerae adaptation to different carbon sources
    JANE MAY LIU; Fiscal Year: 2010
    ..and mutagenesis screens have previously indicated that energy metabolism pathways, particularly the phosphoenolpyruvate (PEP)-carbohydrate phosphotransferase system (PTS), play a major role in the transition of V...
  7. The Role of phosphoenolpyruvate carboxykinase and reactive oxygen species in the
    JASON EARL PODRABSKY; Fiscal Year: 2013
    ..recovery from anoxia, and test the hypothesis that an alternate metabolic pathways supported by the enzyme phosphoenolpyruvate carboxykinase is critical for the survival of anoxia...
  8. Metabolic functions of adipose pyruvate kinase M2
    Ahmed Bettaieb; Fiscal Year: 2013
    ..Pyruvate kinase M2 (PKM2) is primarily a tetrameric enzyme that catalyzes the transfer of a phosphate from phosphoenolpyruvate to ADP, resulting in pyruvate and ATP...
  9. Biosynthesis of Hypotensive Phosphonopeptide Natural Products
    Brian O Bachmann; Fiscal Year: 2010
    ..However, it is difficult to rationalize how phosphoenolpyruvate mutase, the activity responsible for forming all previously studied phosphonate containing natural products,..
  10. The role of the mitochondrial GTP cycle in insulin secretion
    Richard G Kibbey; Fiscal Year: 2013
    ..and must convert anaplerotic metabolites into PEP by the GTP-dependent mitochondrial isoform of phosphoenolpyruvate carboxykinase (PEPCK-M) that then transmits the signal to the cytosol...
  11. Structure and Function of a Phosphorylation Coupled Saccharide Transporter
    Ming Zhou; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The phosphoenolpyruvate-dependent phosphotranferase system (PTS) is a multicomponent carbohydrate uptake system that is also involved in the regulation of metabolism, chemotaxis, and pathogenicity in ..
  12. STRUCTURE/FUNCTION OF MITOCHONDRIAL CITRATE CARRIER
    RONALD KAPLAN; Fiscal Year: 2009
    ..This transporter catalyzes the exchange of tricarboxylates, dicarboxylates, and phosphoenolpyruvate across the inner mitochondrial membrane, and as such is essential to the energy metabolism of eukaryotic ..
  13. MECHANISM OF CARBOHYDRATE TRANSPORT IN BACTERIA
    Milton Saier; Fiscal Year: 2000
    ..The phosphoenolpyruvate:sugar phosphotransferase system (PTS) is a complex transport, kinase and regulatory system which in E...
  14. Regulation of Streptococcus mutans PTS Transporters
    Dragana Ajdic; Fiscal Year: 2013
    ..In S. mutans, sugar substrates are taken up by ABC transporters and, more commonly, by phosphoenolpyruvate (PEP)-sugar phosphotransferase systems (PTSs)...
  15. FRUCTOFURANOSE MODULATORS--STRUCTURAL/ENZYMATIC STUDIES
    EZZAT YOUNATHAN; Fiscal Year: 1992
    ..levels observed as a side effect of the hypoglycemic action of 2,5-AM, two gluconeogenic enzymes, namely phosphoenolpyruvate carboxykinase and malate dehydrogenase will be investigated for sensitivity to inhibition by its ..
  16. INTERMEDIARY METABOLISM OF LEISHMANIA
    JACOB BLUM; Fiscal Year: 1993
    ..We intend to measure the levels of certain key intermediate metabolites such as ATP, glucose-6-phosphate, phosphoenolpyruvate, and fructose-2,6 bisphosphate, as a function of oxygen concentration...
  17. CARNITINE ACYLTRANSFERASE CHARACTERISTICS
    Linda Brady; Fiscal Year: 1993
    ..lines, as well as CPT-deficient human fibroblast lines, with retroviral vector constructs containing the phosphoenolpyruvate carboxykinase (PEPCK) promoter, the mouse mammary tumor virus (MMTV) promoter or the metallothionein ..
  18. Specificity of Streptococcus mutans sugar transporters
    Dragana Ajdic; Fiscal Year: 2009
    ..In S. mutans, sugar substrates are taken up by ABC transporters and by phosphoenolpyruvate (PEP)-sugar phosphotransferase systems (PTSs)...
  19. PHOSPHOENOLPYYRUVATE CARBOXYKINASE IN ADIPOSE TISSUE
    David Loose; Fiscal Year: 1991
    The overall goal of this proposal is to understand the regulation of the gene for phosphoenolpyruvate carboxykinase (GTP) (PEPCK) in adipose tissue. The regulation of PEPCK is unique in its tissue specificity...
  20. MECHANISMS OF ENZYME CATALYSIS
    Jeremy Knowles; Fiscal Year: 1993
    ..acid-7-phosphate synthase (where the role of metal ion, and the curious nature of the enzyme's handling of phosphoenolpyruvate will be probed), and dehydroquinate synthase (for which we shall synthesize a logical sequence of blocked ..
  21. Structure, mechanism and evolution of KDO8P synthase
    DOMENICO GATTI; Fiscal Year: 2007
    ..The first step in the synthesis of KDO is the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) to form KDO 8-phosphate (KDO8P), the phosphorylated precursor of KDO, catalyzed by KDO8P synthase (..
  22. ENZYME INTERMEDIATE STRUCTURES BY NMR
    Michael Smerdon; Fiscal Year: 2002
    ..EPSP synthase catalyzes the condensation of shikimate-3-phosphate and phosphoenolpyruvate, and the product, EPSP, is a key intermediate in the biosynthesis of aromatic amino acids...
  23. ALTERNATIVE ROLES FOR PHOSPHOENOLPYRUVATE CARBOXYKINASE
    Paul Ray; Fiscal Year: 1991
    b>Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the following reaction: oxalacetate(OAA) + GTP yields equilibrium PEP + HCO3- + GDP. Large amounts are in liver, kidney, white and brown adipose tissues...
  24. PHYSIOLOGIC CHARACTERISTICS OF TREPONEMA PALLIDUM
    Steven Norris; Fiscal Year: 2004
    ..Milton Saier an colleagues at the University of California at San Diego will examine the role of the phosphoenolpyruvate dependent phospho-transferase system (PTS) in gene regulation. A unique aspect of the T...
  25. ADAPTIVE ACID TOLERANCE OF STREPTOCOCCUS SOBRINUS
    JOSE LEMOS; Fiscal Year: 2007
    ..by growth in acidic conditions, as it is in many other organisms, and expression of the glucose-specific phosphoenolpyruvate sugar: phosphotransferase system (PTS), which in S...
  26. PHOSPHATE ANALOG PROBES OF RED CELL METABOLISM
    Richard Labotka; Fiscal Year: 1990
    ..dependence of the transport of small molecules containing more than one anionic group (phosphonoformate and phosphoenolpyruvate) to that for anions of similar size containing only one anionic group (ethyl phosphate and acetyl phosphate)..
  27. REGULATION OF TISSUE-SPECIFIC GENE EXPRESSION
    Elmus Beale; Fiscal Year: 1992
    ..This proposal uses the cytosolic phosphoenolpyruvate carboxykinase (PEPCK) gene as a model to accomplish this goal by addressing the mechanisms that allow PEPCK ..
  28. Mechanism of KDO 8-P and DAH 7-P Synthase
    RONALD WOODARD; Fiscal Year: 2004
    ..the formation of 3-deoxy-D-manno-octulosonic 8-phosphate (KDO 8-P) from arabinose 5-phosphate (A 5-P) and phosphoenolpyruvate (PEP) catalyzed by the enzyme KDO 8-P synthase (EC 4.1.2...
  29. STRUCTURAL CHEMICAL STUDIES OF METABOLIC PHOSPHATES
    Robert Blessing; Fiscal Year: 1992
    ..The series ranges from the high-energy phosphoenolpyruvate to the low-energy 3-phosphoglycerate, and includes inorganic orthophosphate and pyrophosphate as well as ..
  30. REGULATION OF HEPATIC PRODUCTION AND UPTAKE OF GLUCOSE
    Stanley Blumenthal; Fiscal Year: 1980
    ..of insulin, diabetes, and diet on glycolysis and lipid syntesis; 2) the possibility that platelets possess phosphoenolpyruvate carboxykinase, pyruvate carboxylase, and malic enzyme and the alterations in enzyme activities caused by ..
  31. TRYPANOSOME GENE EXPRESSION AND ENVIRONMENTAL ADAPTATION
    GEORGE CROSS; Fiscal Year: 1993
    ..The first one to be attempted will be malate dehydrogenase; alternatives include phosphoenolpyruvate carboxykinase and adenylate kinase.
  32. Inhibitors of KD08-Phosphate Synthase as Antibiotics
    David Green; Fiscal Year: 2001
    ..key step in the biosynthesis of the outer membrane lipopolysaccharide component, namely the condenstaion of phosphoenolpyruvate and arabinose-5-phosphate to form 3-deoxy-D-mann-octulosonic-8-phosphate...
  33. NUCLEAR PROTEIN KINASES AND GENE EXPRESSION
    RICHARD JUNGMANN; Fiscal Year: 1990
    ..transcriptional induction of LDH mRNA, and the rat H4IIE hepatoma cell line in which the induction of the phosphoenolpyruvate carboxykinase gene is regulated by cAMP and insulin...
  34. RETINOIC ACID REGULATION OF GENE EXPRESSION
    Mary McGrane; Fiscal Year: 1999
    ..In order to do this, a retinoid- responsive gene, that for phosphoenolpyruvate carboxykinase (PEPCK), has been introduced into transgenic mice...
  35. Role of Perinatal Nutrition in the Development of Insulin Resistance
    Colleen Croniger; Fiscal Year: 2009
    ..insulin resistance, using a mouse model with a deletion of the PPAR binding site in the cytosolic form of phosphoenolpyruvate carboxykinase (PEPCK-C) gene promoter (PPARE-/- mice)...
  36. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    Low G+C Gram-positive bacteria possess the phosphoenolpyruvate:sugar phosphotransferase system (PTS) which transports and initiates the metabolism of its sugar substrates and influences the processes of catabolite repression (CR) and ..
  37. Proteomics to screen for a protein kinase that uses PEP
    GERALD CARLSON; Fiscal Year: 2006
    ..with much greater potential for phosphoryl transfer than ATP occur in our cells; one such compound is phosphoenolpyruvate (PEP), which has a phosphoryl transfer capability twice that of ATP...
  38. BIOSYNTHESIS OF THE ESCHERICHIA COLI LIPOPOLYSACCHARIDE
    DOMENICO GATTI; Fiscal Year: 2002
    ..The reaction, with involves the condensation of phosphoenolpyruvate (PEP) with arabinose 5-(A5P) to yield KDO8P and Pi, is poorly understood at present...
  39. ISOZYME INTERRELATIONSHIPS IN GLUCONEOGENESIS
    ROBERT SWICK; Fiscal Year: 1980
    ..will be accomplished by a study of the varied distribution patterns of the alanine aminotransferase and phosphoenolpyruvate carboxykinase isoenzymes in the gluconeogenic tissues from a number of species...
  40. KINETICS AND CHEMISTRY OF ALLOSTERIC INTERACTIONS
    Thomas Smith; Fiscal Year: 1992
    Our objectives are to define the mechanism of action and regulation of the enzyme phosphoenolpyruvate (PEP) carboxylase of Escherichia coli, and the structural requirements for those functions...
  41. PHOSPHOENOLPYRUVATE: GLYCOSE PHOSPHOTRANSFERASE SYSTEM
    Saul Roseman; Fiscal Year: 2004
    ..The E. coli Glc (or Me alpha-glucoside (MeGlc)) permease is part of the phosphoenolpyruvate:glycose phosphotransferase system (PTS), and catalyzes simultaneous Glc uptake and phosphorylation...
  42. NEW METHODS IN SOLID STATE CRYOENZYMOLOGY
    Jeremy Evans; Fiscal Year: 1990
    ..EPSP) synthase, which catalyses the condensation of shikimate-3-phosphate and phosphoenolpyruvate, will be used as a test case for the development of this novel technique of cryoenzymological solid-state ..
  43. EFFECT OF MATERNAL DIABETES ON METABOLISM IN NEWBORNS
    Mulchand Patel; Fiscal Year: 1980
    ..Specifically, alterations in hepatic glycogen levels and blood metabolites as well as induction of phosphoenolpyruvate carboxykinase, a key gluconeogenic enzyme, in developing liver are being investigated...
  44. Shikimate Pathway Variants
    John Frost; Fiscal Year: 2005
    ..A case in point is the shikimate pathway. Starting with the condensation of phosphoenolpyruvate with D-erythrose 4-phosphate, the shikimate pathway has been the focus of intense scrutiny...
  45. A Chaperonin/Osmolyte Protein Folding Screen - STTR Phase I
    Mark Fisher; Fiscal Year: 2007
    ..These studies will use dimeric protein ?5-3-ketosteriod isomerase and human mitochondrial phosphoenolpyruvate carboxykinase. The former is a well characterized and crystallized protein; the latter is not...
  46. PEP CARBOXYKINASE IN SUDDEN INFANT DEATH SYNDROME
    Richard Silverstein; Fiscal Year: 1980
    b>Phosphoenolpyruvate carboxykinase is being purified from infant livers of SIDS victims...
  47. GLUTAMINE METABOLISM IN HUMAN CULTURED FIBROBLASTS
    H Zielke; Fiscal Year: 1980
    ..The enzymes of the malate-aspartate shuttle as well as phosphoenolpyruvate carboxykinase, pyruvate carboxylase, malic enzyme are involved in this route of glutamine catabolism...
  48. REGULATION OF GENE EXPRESSION BY NF-KB AND TLE1
    Mary Law; Fiscal Year: 2003
    ..Dr. Granner's laboratory studies the hormonal regulation of phosphoenolpyruvate carboxykinase (PEPCK) gene expression and Dr...
  49. ROLE OF GENE EXPRESSION IN GLUCONEOGENESIS
    Arlan Richardson; Fiscal Year: 1990
    ..of the research described in this proposal is to determine if the age-related decline in the expression of phosphoenolpyruvate carboxykinase (PEPCK) in liver of rats is due to an alteration in the genetic apparatus of the hepatocyte...
  50. Mechanisms and Evolutionary Potential of Promiscuous Enzyme Activities
    SHELLEY COPLEY; Fiscal Year: 2007
    ..Aim 1. To explore the mechanistic basis of the promiscuous activities of phosphoenolpyruvate (PEP) synthase and dihydroquinate (DHQ) synthase. Aim 2...
  51. MECHANISMS OF HEAT SHOCK PROTECTION FROM INFLAMMATION
    Charles Paidas; Fiscal Year: 2001
    ..hepatic glucose metabolism). Prior studies have indicated that expression of phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme for gluconeogenesis, is suppressed during inflammation...
  52. The Role of 11beta-HSD1 in Type II Diabetes and Obesity
    Yanjun Liu; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  53. Phthalate Exposure and Pregnancy Outcome
    T Peter Stein; Fiscal Year: 2010
    ..The findings will have ramifications for public health, for the packaging trade and for the chemical industry. ..
  54. HORMONAL REGULATION OF ENZYME LEVELS IN MAMMALIAN TISSUE
    Richard Hanson; Fiscal Year: 2009
    DESCRIPTION (provided by applicant) The cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (PEPCK-C) is critical for energy metabolism;it ablation results in death within 2 days after birth, with massive accumulation of lipid in ..
  55. Lipoprotein Metabolism 2008 Gordon Research Conference
    Robert Hegele; Fiscal Year: 2008
    ..End of Abstract) [unreadable] [unreadable] [unreadable]..
  56. Universal Technology for Profiling the Dynamics of Normal & Oncogenic Signaling
    Karen Anderson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  57. Proteomic Analysis of Renal Response to Acidosis
    Norman Curthoys; Fiscal Year: 2008
    ..In addition to glutaminase (GA), glutamate dehydrogenase (GDH), and phosphoenolpyruvate carboxykinase (PEPCK), three well- characterized proteins that are induced in response to acidosis, this ..
  58. A Molecular Approach For Opportunistic HIV-1 Infections
    Karen Anderson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  59. LOCAL AND LONG RANGE INTERACTIONS IN PROTEIN FOLDING
    J Scholtz; Fiscal Year: 2004
    ..abstract_text> ..
  60. MECHANISTIC STUDIES ON PEP UTILIZING ENZYMES
    Karen Anderson; Fiscal Year: 2003
    ..In addition as the investigator develops the quantitative aspects of the rapid mixing, pulsed-flow ESI-MS technique further, she will extend the studies of these enzymes to determine the full kinetic profile. ..