dithionitrobenzoic acid

Summary

Summary: A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.

Top Publications

  1. ncbi Inactivation of hantaviruses by N-ethylmaleimide preserves virion integrity
    Tomas Strandin
    Peptide and Protein Laboratory, Infection Biology Research Program, Haartman Institute, University of Helsinki, Helsinki, Finland
    J Gen Virol 92:1189-98. 2011
  2. ncbi The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell binding
    E Fenouillet
    Centre National de la Recherche Scientifique, Faculte de Medecine Nord, Marseille, France
    J Infect Dis 183:744-52. 2001
  3. pmc Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein
    Surbhi Jain
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Virol 81:2328-39. 2007
  4. pmc Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction
    H J Ryser
    Department of Pathology, Boston University School of Medicine, MA 02118
    Proc Natl Acad Sci U S A 91:4559-63. 1994
  5. pmc Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entry
    Uthaman Gowthaman
    Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
    BMC Bioinformatics 9:S14. 2008
  6. pmc Antiviral propierties of 5,5'-dithiobis-2-nitrobenzoic acid and bacitracin against T-tropic human immunodeficiency virus type 1
    Humberto H Lara
    Laboratorio de Inmunología y Virología, Departamento de Microbiologia e Inmunologia, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico
    Virol J 8:137. 2011
  7. ncbi Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9[93]beta sulfhydryl groups of the hemoglobins of the domestic cat: variation of the equilibrium and reverse rate constants with pH
    Kehinde Onwochei Okonjo
    Department of Chemistry, University of Ibadan, Ibadan, Nigeria
    Biophys Chem 121:65-73. 2006
  8. ncbi On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobin
    Z Feng
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 314:153-66. 2001
  9. ncbi Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis
    O Bjornberg
    Center for Enzyme Research, Institute of Molecular Biology, and Centre for Crystallograpic Studies, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
    Biochemistry 36:16197-205. 1997
  10. ncbi Regulation of PTP1B via glutathionylation of the active site cysteine 215
    W C Barrett
    Laboratory of Biochemistry, Section of Metabolic Regulation, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892, USA
    Biochemistry 38:6699-705. 1999

Research Grants

  1. Calorimetry Work Station
    SHERWIN LEHRER; Fiscal Year: 2002
  2. TROPOMYOSIN AND THE REGULATION OF MUSCLE CONTRACTION
    SHERWIN LEHRER; Fiscal Year: 2009
  3. Anesthetics block neuronal voltage-gated Ca2+ currents
    SLOBODAN TODOROVIC; Fiscal Year: 2009
  4. ABCA1 agonist peptides for therapeutic use
    JOHN BIELICKI; Fiscal Year: 2008
  5. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
  6. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
  7. ApoA-I Helical Domains and Cardiovascular Aging
    JOHN BIELICKI; Fiscal Year: 2005
  8. Acetylcholinesterase: A Comparative Biochemical Approach
    Leo Pezzementi; Fiscal Year: 2005
  9. MULTIPLE CLASSES OF CA2+ CHANNELS - SENSORY TRANSMISSION
    SLOBODAN TODOROVIC; Fiscal Year: 2004
  10. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004

Detail Information

Publications176 found, 100 shown here

  1. ncbi Inactivation of hantaviruses by N-ethylmaleimide preserves virion integrity
    Tomas Strandin
    Peptide and Protein Laboratory, Infection Biology Research Program, Haartman Institute, University of Helsinki, Helsinki, Finland
    J Gen Virol 92:1189-98. 2011
    ..In contrast to hantaviruses, NEM did not abolish Uukuniemi phlebovirus infectivity to the same extent. This indicates differences in the use of free thiols in virus entry among members of the family Bunyaviridae...
  2. ncbi The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell binding
    E Fenouillet
    Centre National de la Recherche Scientifique, Faculte de Medecine Nord, Marseille, France
    J Infect Dis 183:744-52. 2001
    ..The fact that the PDI catalytic activity present on lymphocytes is required for fusion supports the hypothesis that catalysts assist post-CD4 cell binding conformational changes within Env...
  3. pmc Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein
    Surbhi Jain
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Virol 81:2328-39. 2007
    ..In the presence of HN protein, only the reduced form may proceed to refold into additional intermediates, leading to the fusion of membranes...
  4. pmc Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction
    H J Ryser
    Department of Pathology, Boston University School of Medicine, MA 02118
    Proc Natl Acad Sci U S A 91:4559-63. 1994
    ..These findings suggest additional approaches to impede cell infection by HIV...
  5. pmc Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entry
    Uthaman Gowthaman
    Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
    BMC Bioinformatics 9:S14. 2008
    ..This study attempts to perceive the mode of binding of dithionitrobenzoic acid (DTNB), and its structurally related compounds on PDI enzyme.
  6. pmc Antiviral propierties of 5,5'-dithiobis-2-nitrobenzoic acid and bacitracin against T-tropic human immunodeficiency virus type 1
    Humberto H Lara
    Laboratorio de Inmunología y Virología, Departamento de Microbiologia e Inmunologia, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico
    Virol J 8:137. 2011
    ..These drugs proved to be leading compounds for further studies against HIV showing antiviral characteristics of interest...
  7. ncbi Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9[93]beta sulfhydryl groups of the hemoglobins of the domestic cat: variation of the equilibrium and reverse rate constants with pH
    Kehinde Onwochei Okonjo
    Department of Chemistry, University of Ibadan, Ibadan, Nigeria
    Biophys Chem 121:65-73. 2006
    ..2+/-0.2 and 9.4+/-0.1 in the major hemoglobin and 6.7+/-0.3 and 8.4+/-0.1 in the minor hemoglobin...
  8. ncbi On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobin
    Z Feng
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 314:153-66. 2001
    ..The C73 thiol group is exposed by a low-energy fluctuation that does not expose its amide group to exchange...
  9. ncbi Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis
    O Bjornberg
    Center for Enzyme Research, Institute of Molecular Biology, and Centre for Crystallograpic Studies, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
    Biochemistry 36:16197-205. 1997
    ..The possible acid-base chemistry of Cys 130 is compared to previous work on mammalian dihydropyrimidine dehydrogenases, flavoenzymes, which catalyze the reversed reaction, namely the reduction of pyrimidine bases...
  10. ncbi Regulation of PTP1B via glutathionylation of the active site cysteine 215
    W C Barrett
    Laboratory of Biochemistry, Section of Metabolic Regulation, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892, USA
    Biochemistry 38:6699-705. 1999
    ..Glutathionylation of the cysteine sulfenic derivative will prevent the enzyme from further oxidation to its irreversible forms, and constitutes an efficient regulatory mechanism...
  11. ncbi Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagent
    R Daniel
    Laboratoire de Bioorganique et Biotechnologies associé au Centre National de la Recherche Scientifique, Ecole Nationale Superieure de Chimie de Paris, 11 rue P and M Curie, 75231 Paris Cedex 05, France
    J Biol Chem 272:26934-9. 1997
    ..Consistent with literature data, we assume that cysteine 221 and cysteine 265, along with the neighboring cysteines 263 and 268 arranged in cluster, are potential ligands of structural zinc of E. coli dihydroorotase...
  12. ncbi Roles for cysteine residues in the regulatory CXXC motif of human mitochondrial branched chain aminotransferase enzyme
    Myra E Conway
    Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, North Carolina 27157, USA
    Biochemistry 43:7356-64. 2004
    ..Thus, C315 appears to be the sensor for redox regulation of BCAT activity, whereas C318 acts as the "resolving cysteine", allowing for reversible formation of a disulfide bond...
  13. ncbi Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid)
    M J Nozal
    Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain
    J Chromatogr A 778:347-53. 1997
    ..The detection limits are 0.21, 0.92 and 0.61 mumol/g wet sample for glutathione, cysteine and N-acetylcysteine, respectively...
  14. ncbi Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
    Arch Biochem Biophys 421:42-53. 2004
    ..The stoichiometry of histidine modification and the extent of inactivation showed that two essential histidine residues per active site are necessary for complete bovine liver OSC activity...
  15. ncbi Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: an example of application for protein chemistry
    Go Hasegawa
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    J Biochem Biophys Methods 63:33-42. 2005
    ..These data showed that IADC is a useful reagent to specifically identify the positions of a Cys residue(s) in proteins...
  16. ncbi Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1
    J Y Hwang
    , , Germany
    FEBS Lett 508:355-9. 2001
    ..9 microM for Ca(2+) binding to this site with a fast off-rate (t approximately 2 ms). We conclude that the rapid dissociation of Ca(2+) from EF-hand 3 in GCAP-1 triggers activation of guanylate cyclase in rod cells...
  17. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
    ..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
  18. ncbi Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase--a potential target for antimicrobial agents against Pseudomonas aeruginosa
    Roberto Velasco-García
    Laboratorio de Osmorregulación, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Avenida de Los Barrios, Tlalnepantla, Estado de Mexico, 54090 Mexico, Mexico
    Biochem Biophys Res Commun 341:408-15. 2006
    ..methyl-methanethiosulfonate inactivated the enzymes without affecting their stability while the bulkier dithionitrobenzoic acid (DTNB) and bis[diethylthiocarbamyl] disulfide (disulfiram) induced enzyme dissociation--at 23 degrees C--..
  19. ncbi Implications of the role of reactive cystein in arginine kinase: reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified arginine kinase reactivated by dithiothreitol
    Ji Cheng Pan
    Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, PR China
    Biochem Biophys Res Commun 317:539-44. 2004
    ..The reactive cysteine of arginine kinase may play an important role not in the binding to the transition-state analog but in the conformational changes caused by the transition-state analog...
  20. ncbi Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activity
    J Lee
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 276:14804-13. 2001
    ..Taken together, these results demonstrate that both histidines assume critical roles for catalysis...
  21. ncbi Modifications of cysteine residues in the solution and membrane-associated conformations of phosphatidylinositol transfer protein have differential effects on lipid transfer activity
    J M Tremblay
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
    Biochemistry 40:9151-8. 2001
    ..Furthermore, the inhibition of PITP activity by thiol-modifying reagents is a result of steric hindrance of phospholipid substrate binding...
  22. ncbi Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implications
    R Rossi
    Department of Neuroscience, Pharmacology Unit, Via A. Moro 4, University of Siena, Italy
    J Biol Chem 276:7004-10. 2001
    ..This is thought to influence significantly the biochemical, pharmacological, and toxicological effects of some drugs...
  23. pmc Structural and functional studies of vertebrate metallothioneins: cross-talk between domains in the absence of physical contact
    Clemente Capasso
    CNR, Institute of Protein Biochemistry, via P Castellino 111 I 80131 Naples, Italy
    Biochem J 391:95-103. 2005
    ..Taken together, these results provide a strong argument in favour of the interaction of the two domains in the MT molecule, in spite of the elusive evidence provided by the structural analyses...
  24. ncbi Changes in sulfhydryl content of egg white proteins due to heat and pressure treatment
    Iesel Van der Plancken
    Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B 3001 Heverlee, Belgium
    J Agric Food Chem 53:5726-33. 2005
    ..Treatment conditions resulting in extensive protein insolubilization were accompanied by a transfer of free sulfhydryl groups from the soluble to the insoluble protein fraction. These SH groups were mainly accessible to DTNB...
  25. ncbi The role of cysteine residues in tellurite resistance mediated by the TehAB determinant
    M Dyllick-Brenzinger
    Structural Biology Research Group, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
    Biochem Biophys Res Commun 277:394-400. 2000
    ..Studies using native and SDS denaturing PAGE under reducing and oxidizing conditions suggested that a cysteine in TehB is involved in binding tellurite...
  26. ncbi The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid)
    Zhao Feng
    Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
    Int J Biol Macromol 36:184-90. 2005
    ..The reactive cysteine of AK, which was proposed to be Cys274, may play an important role not in the binding of the transition-state analog but in the conformational changes caused by the transition-state analog...
  27. ncbi Cleavage of disulfide-linked fetuin-bisphosphonate conjugates with three physiological thiols
    Sufeng Zhang
    Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB T6G 2G6, Canada
    Biomacromolecules 6:2800-8. 2005
    ..In conclusion, disulfide-linked fetuin-thiolBP conjugates exhibited a high affinity to HA, which was readily lost upon cleavage with thiols found in physiological milieu...
  28. ncbi Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple form
    Kehinde O Okonjo
    Department of Chemistry, University of Ibadan, Ibadan, Nigeria
    Biophys Chem 119:196-204. 2006
    ..Analyses of the simple profiles gave pKas of 6.33 +/- 0.17 and 8.54 +/- 0.5 for HisH21[143]beta and other histidines within a distance of 2 nm of the sulfhydryl, and CysF9[93]beta of the minor hemoglobin, respectively...
  29. pmc Zinc transfer potentials of the alpha - and beta-clusters of metallothionein are affected by domain interactions in the whole molecule
    L J Jiang
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Seeley G Mudd Building, 250 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:2503-8. 2000
    ..The two-domain structure of the whole molecule is important for its interaction with ligands and for control of its reactivity and overall conformation...
  30. ncbi Development and application of serum cholinesterase activity measurement using benzoylthiocholine iodide
    Susumu Osawa
    School of Medicine, Biological Sciences and Technology, Department of Health Sciences, Kyushu University, Higashi, Fukuoka City 812 8582, Japan
    Clin Chim Acta 351:65-72. 2005
    ..Many procedures using various artificial substrates have been developed but suffer from problems with substrate specificity and interference by endogenous substances...
  31. ncbi Perturbation of microtubule polymerization by quercetin through tubulin binding: a novel mechanism of its antiproliferative activity
    Kamlesh Gupta
    BJM School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India
    Biochemistry 41:13029-38. 2002
    ..The data suggest that quercetin inhibits cancer cells proliferation at least in part by perturbing microtubule functions through tubulin binding...
  32. ncbi A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGK
    Matthew J Cliff
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
    J Mol Biol 364:810-23. 2006
    ..The success of the competition technique in characterizing this kinetic intermediate invites application to other model systems...
  33. ncbi Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinase
    Hua Wei He
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
    Int J Biol Macromol 41:361-8. 2007
    ..Furthermore, the findings in this study may provide a novel explanation for the low pKa value of Cys283...
  34. ncbi Critical flocculation concentrations, binding isotherms, and ligand exchange properties of peptide-modified gold nanoparticles studied by UV-visible, fluorescence, and time-correlated single photon counting spectroscopies
    Huan Xie
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Anal Chem 75:5797-805. 2003
    ..Full characterization of the composition and stability of peptide-modified metal nanoparticles is an important step in their use as intracellular delivery vectors and imaging agents...
  35. pmc Antiviral activity of RhoA-derived peptides against respiratory syncytial virus is dependent on formation of peptide dimers
    Philip J Budge
    Department of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Antimicrob Agents Chemother 47:3470-7. 2003
    ....
  36. pmc Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells
    C H Davis
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA
    Infect Immun 70:3413-8. 2002
    ..trachomatis serovar E entry...
  37. ncbi Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Free Radic Biol Med 35:1185-93. 2003
    ....
  38. ncbi Role of cysteine 306 in the catalytic mechanism of Ascaris suum phosphoenolpyruvate carboxykinase
    Sandra E Rios
    Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA
    Arch Biochem Biophys 404:25-37. 2002
    ..Alternatively, mutation of this residue could cause protein changes that interfere with the proper conformation of the nucleotides for optimal catalysis to take place...
  39. ncbi The roles of thiols in the bacterial organomercurial lyase (MerB)
    Keith E Pitts
    Department of Microbiology and Center for Metalloenzyme Studies, The University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 41:10287-96. 2002
    ..On the basis of the foregoing we refine the previously proposed S(E)2 mechanism for protonolysis by MerB...
  40. ncbi Single-molecule covalent chemistry with spatially separated reactants
    Tudor Luchian
    Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843 1114, USA
    Angew Chem Int Ed Engl 42:3766-71. 2003
  41. pmc Kinetic studies of rat liver hexokinase D ('glucokinase') in non-co-operative conditions show an ordered mechanism with MgADP as the last product to be released
    Octavio Monasterio
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Biochem J 371:29-38. 2003
    ..In addition, the straight-line dependence observed when the reciprocal value of the inactivation constant was plotted against the sugar-ligand concentration supports the view that there is just one sugar-binding site in hexokinase D...
  42. ncbi Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coli
    M Yamaguchi
    J Biochem 86:325-33. 1979
    ..Based on the above results, the mechanism of the alteration of enzyme activities by sulfhydryl group modification is discussed...
  43. ncbi Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W
    M Iwakura
    J Biochem 86:1239-49. 1979
    ..The protective effect of NADP+ was increased by the addition of Mg2+. Based on the above results, the effects of Mg2+ on the SH-group modification are discussed from the viewpoint of conformational alteration of the enzyme...
  44. ncbi Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1
    Judith Lahav
    The Coagulation Laboratory, Rabin Medical Center Beilinson Campus, Zabotinski St, Petah Tiqva 49100, Israel
    Blood 102:2085-92. 2003
    ..These data suggest that the enzymatically catalyzed adhesion-associated reorganization of disulfide bonds is common to members of the integrin family and specific to this family...
  45. ncbi Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coli
    S R Dowd
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1252:278-83. 1995
    ..Cysteine 492 has been identified as containing the sulfhydryl that is accessible to the reagent under both conditions...
  46. ncbi Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coli
    M M Altamirano
    Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, D F
    Arch Biochem Biophys 258:95-100. 1987
    ..The results obtained show that sulfhydryls are not essential for catalysis or allosteric behavior of glucosamine-6-phosphate deaminase...
  47. pmc Contribution of redox status to hepatitis C virus E2 envelope protein function and antigenicity
    Emmanuel Fenouillet
    UMR 6184, CNRS, Université Aix Marseille II, F 13015, France
    J Biol Chem 283:26340-8. 2008
    ..Although E2 function is surprisingly independent of its redox status, its disulfide bonds mask antigenic domains. E2 redox manipulation may contribute to improved vaccine design...
  48. ncbi Characterization of chitosan thiolation and application to thiol quantification onto nanoparticle surface
    I Bravo-Osuna
    Laboratoire de Physicochimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Universite Paris Sud 11, Faculte de Pharmacie, 5, rue J B Clement, 92296 Chatenay Malabry, France
    Int J Pharm 340:173-81. 2007
    ..It was successfully applied to the quantification of active thiol groups on the surface of poly(isobutyl cyanoacrylates) nanoparticles coated with thiol chitosan...
  49. pmc Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase system
    Thomas J Jönsson
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Biochemistry 46:5709-21. 2007
    ..In addition, two new constructs have been generated, an AhpF-AhpC complex and a "twisted" form of AhpF, in which redox centers are locked together by stable disulfide bonds which mimic catalytic intermediates...
  50. ncbi Simple shifts in redox/thiol balance that perturb blood coagulation
    Henry K Bayele
    Department of Haematology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
    FEBS Lett 510:67-70. 2002
    ..These data also suggest that blood clotting may be influenced by unidentified redox or thiol equilibria...
  51. ncbi Identification and characterization of a critical region in the glycogen synthase from Escherichia coli
    Alejandra Yep
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 279:8359-67. 2004
    ..The results of this work indicate that this region is critical for catalysis and substrate binding...
  52. ncbi Reduced redox state allows prolonged survival of axotomized neonatal retinal ganglion cells
    L K Geiger
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, 600 Highland Avenue, Madison, WI 53792, USA
    Neuroscience 109:635-42. 2002
    ..Understanding the mechanisms by which ROS signal neuronal death could result in strategies for increasing their long-term survival after axonal injury...
  53. ncbi Role of cysteine residues in 4-oxalomesaconate hydratase from Pseudomonas ochraceae NGJ1
    Suhong Li
    Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
    Biosci Biotechnol Biochem 71:449-57. 2007
    ....
  54. ncbi Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liver
    Hao Chen
    Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22, 14195 Berlin Dahlem, Germany
    Glycobiology 12:65-71. 2002
    ....
  55. ncbi Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site
    Wen Jing Zhu
    College of Life Science, Shandong Agricultural University, Shandong Taian 271018, People s Republic of China
    Int J Biol Macromol 41:564-71. 2007
    ..Using the specific chemical modifier phenylglyoxal (PG) demonstrated that only one Arg, located near the ATP binding site, is essential for the activity of AK...
  56. ncbi Recombinant lysine:N(6)-hydroxylase: effect of cysteine-->alanine replacements on structural integrity and catalytic competence
    Scott Dick
    Department of Chemistry, University of Waterloo, ON, Canada
    Biochim Biophys Acta 1594:219-33. 2002
    ..A possible role of rIucD's Cys51 in the modulation of its mono-oxygenase function is discussed...
  57. pmc Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bonds
    Jane E Raulston
    Department of Microbiology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0579, USA
    Infect Immun 70:535-43. 2002
    ..The accessibility of the Hsp70 substrate-binding domain may support the hypothesis that this protein plays a role in events following the initial stage of attachment instead of serving as a primary, surface-displayed adhesin...
  58. pmc DNA damage, apoptosis and cell cycle changes induced by fluoride in rat oral mucosal cells and hepatocytes
    Ling Fei He
    Department of Dental Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
    World J Gastroenterol 12:1144-8. 2006
    ..To study the effect of fluoride on oxidative stress, DNA damage and apoptosis as well as cell cycle of rat oral mucosal cells and hepatocytes...
  59. pmc Comparative inhibition by hard and soft metal ions of steroid-binding capacity of renal mineralocorticoid receptor cross-linked to the 90-kDa heat-shock protein heterocomplex
    M D Galigniana
    Departamento de Qu approximately ímica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, and PHROM CONICET, 1428 Buenos Aires, Argentina
    Biochem J 341:585-92. 1999
    ..role played by these residues in steroid binding was confirmed by chemical modification of cysteines with dithionitrobenzoic acid, histidines with diethyl pyrocarbonate and acidic amino acids with Woodward's reagent (N-ethyl-5-..
  60. ncbi Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages
    Min Kyu Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    J Vet Med Sci 69:627-32. 2007
    ..GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay...
  61. ncbi Irreversible activation of cyclic nucleotide-gated ion channels by sulfhydryl-reactive derivatives of cyclic GMP
    R L Brown
    Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, Oregon 97209, USA
    Biochemistry 39:432-41. 2000
    ..Pretreatment of the patch with the sulfhydryl-blocking reagents N-ethylmaleimide (NEM) and bis-dithionitrobenzoic acid (DTNB) prevented covalent activation; the effect of DTNB was reversed by reduction with DTT...
  62. ncbi Effect of oxidative stress on the structure and function of human serum albumin
    M Anraku
    Faculty of Pharmaceutical Sciences, Kumamoto University, Japan
    Pharm Res 18:632-9. 2001
    ..In contrast, both the ligand binding property of site II and the esterase-like activity of oxidized HSAs are decreased, most probably due to conformational changes in subdomain IIIA...
  63. ncbi Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii Glover)
    Fei Li
    Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China
    Arch Insect Biochem Physiol 51:37-45. 2002
    ..43 and 11.73 times, respectively. We infer that one or more factors in the crude extract from the resistance strain have more influence on AChE sensitivity. Further study is needed to investigate the basis of these observations...
  64. ncbi Interaction between catalytic and regulatory sites of the pyruvate dehydrogenase from Escherichia coli studied by the ESR technique
    K Graupe
    Physiologisch Chemisches Institut, Universitat Tubingen, F R G
    Biochim Biophys Acta 999:176-82. 1989
    ..The implications of these results for the allosteric regulation of the pyruvate dehydrogenase complex are discussed...
  65. ncbi Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes
    G Gao
    Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
    J Biol Chem 269:8234-9. 1994
    ..Residues 5-12 and 15 of this peptide are identical with a sequence of a yeast alkaline p-nitrophenylphosphatase, and the peptide has little homology with other mammalian phosphatases...
  66. ncbi Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol
    Robert M Willis
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, United States
    Biochemistry 50:10550-8. 2011
    ..The substrate cis-11-hexadecenal was reduced in a cooperative manner with an apparent K(m) of ~50 μM, V(max) of ~8 μmol NADP(+) min(-1) (mg protein)(-1), and n ~ 2...
  67. ncbi New findings about Ellman's method to determine cholinesterase activity
    Alena Komersová
    Department of Physical Chemistry, Faculty of Chemical Technology, University of Pardubice, Nám Legií 565, 532 10 Pardubice, Czech Republic
    Z Naturforsch C 62:150-4. 2007
    ..Our experiments indicate that this influence of DTNB concentration can be explained by the inhibition of ATCH hydrolysis by DTNB...
  68. ncbi Effect of cadmium, mercury and copper on partially purified hepatic flavokinase of rat
    D Bandyopadhyay
    Indian Institute of Chemical Biology, Calcutta, India
    Mol Cell Biochem 167:73-80. 1997
    ....
  69. pmc Catalytic zinc site and mechanism of the metalloenzyme PR-AMP cyclohydrolase
    Robert L D'Ordine
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
    Biochemistry 51:5791-803. 2012
    ..The results are consistent with a Zn(2+)-mediated activation of H(2)O mechanism involving histidine as a general base that has features similar to but distinct from those of previously characterized purine and pyrimidine deaminases...
  70. ncbi Evaluation of protein disulfide conversion in vitro using a continuous flow dialysis system
    Xinzhao Grace Jiang
    Drug Substance Development, Amgen, Thousand Oaks, CA 91320, USA
    Anal Biochem 432:142-54. 2013
    ..These are important considerations when evaluating the criticality of product quality attributes...
  71. ncbi Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistry
    Arlin B Rogers
    Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Lab Invest 86:526-33. 2006
    ..These findings provide a molecular basis to improve the specificity of IHC and other immunoassays, and hold implications for antibody-based clinical diagnostics and therapeutics...
  72. pmc Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerization
    P J Britto
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem J 389:549-58. 2005
    ..Under such conditions, the substituted tubulin forms protofilament-based structures such as microtubules, open tubules, sheets and/or bundles...
  73. ncbi Purification of acetylcholinesterase by 9-amino-1,2,3,4-tetrahydroacridine from human erythrocytes
    Habibe Budak Kaya
    Department of Chemistry, Faculty of Sciences, Ataturk University, 25240 Erzurum, Turkey
    Appl Biochem Biotechnol 170:198-209. 2013
    ..25 mM, and the V(max) was 0.090 μmol/mL/min. Maximum binding was achieved at 2 °C with pH 7.4 and an ionic strength of approximately 0.1 M. The capacity for the optimum condition was 0.07 mg protein/g gel for acetylcholinesterase...
  74. ncbi Nitric oxide directly activates GABA(A) receptor function through a cGMP/protein kinase-independent pathway in frog pituitary melanotrophs
    H Castel
    European Institute for Peptide Research (IFRMP 23, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France
    J Neuroendocrinol 13:695-705. 2001
    ..The effect of NO can be accounted for by S-nitrosylation/oxidation of thiol groups either directly on the GABA(A) receptor subunits or on a regulatory protein tightly associated with the GABA(A) receptor...
  75. ncbi A colorimetric microplate assay method for high throughput analysis of lipase activity
    Suk Jung Choi
    Department of Chemistry, Kangnung National University, Gangneung 210 702, Korea
    J Biochem Mol Biol 36:417-20. 2003
    ..01-0.1 U or 1-10 U. When assaying crude tissue extracts, the reaction of DTNB with non-specific reducing agents created a major source of error. However, this error was corrected by the use of blank samples that did not contain DMPTB...
  76. ncbi A simple assay of taurine concentrations in food and biological samples using taurine dioxygenase
    Motoki Matsuda
    Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Anal Biochem 427:121-3. 2012
    ..Taurine dioxygenase can be used for taurine determination in food control, biological research, and diagnoses based on urinary taurine concentration...
  77. ncbi HIV-1 viral load determination based on reverse transcriptase activity recovered from human plasma
    Anders Malmsten
    Department of Genetics and Pathology, Uppsala University, and Cavidi Tech AB, Uppsala Science Park, SE 751 83 Uppsala, Sweden
    J Med Virol 71:347-59. 2003
    ..Being insensitive to genetic drift occurring in HIV, the assay should be of particular use in resource-limited settings, where different subtypes and recombinant HIV strains occur...
  78. ncbi Soybean (Glycine max) urease: significance of sulfhydryl groups in urea catalysis
    Sandeep Kumar
    School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
    Plant Physiol Biochem 48:746-50. 2010
    ..Finally, when NEM inactivated urease was incubated with sodium fluoride, a time-dependent regain of activity was observed with higher concentrations of fluoride ion...
  79. ncbi Purification and characterization of camel (Camelus dromedarius) milk amylase
    Esmail M El-Fakharany
    Antibody Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, New Borg EL Arab, Alexandria, Egypt
    Prep Biochem Biotechnol 39:105-23. 2009
    ..It worked on starch to yield a maltose. Scanning electron microscope images demonstrated a nano-degrading ability on starch granules from various sources (potato, corn, cassava, and rice)...
  80. ncbi [Determination of L-cysteine in enzymatic reaction mixture with pre-column derivatization by high performance liquid chromatography]
    Zhong Liu
    College of Life Science, Nankai University, Tianjin 300071, China
    Se Pu 22:231-3. 2004
    ..The linear range was 5-950 micromol/L. The recoveries were 99.7%-100.5% and the relative standard deviations (RSDs) were less than 1.3%. The detection limit was 0.8 micromol/L. The method is simple and accurate...
  81. ncbi Ascorbic acid reduction of microtubule protein disulfides and its relevance to protein S-nitrosylation assays
    Lisa M Landino
    Department of Chemistry, The College of William and Mary, P O Box 8795, Williamsburg, VA 23187 8795, USA
    Biochem Biophys Res Commun 340:347-52. 2006
    ..Not only do we describe a new reactivity of ascorbic acid with microtubule proteins but we expose an important limitation when using the biotin switch assay to detect protein S-nitrosylation...
  82. ncbi Species-specific inhibition of Giardia lamblia triosephosphate isomerase by localized perturbation of the homodimer
    Sergio Enriquez-Flores
    Laboratorio de Bioquímica Genética y Dirección de Investigación, Instituto Nacional de Pediatria, 04530 Mexico, DF, Mexico
    Mol Biochem Parasitol 157:179-86. 2008
    ..The effect of thiol reagents on human TIM was also assayed; it is 180-fold less sensitive than GlTIM. Collectively, the data illustrate GlTIM as a good target for drug design...
  83. ncbi Selective optical sensing of biothiols with Ellman's reagent: 5,5'-Dithio-bis(2-nitrobenzoic acid)-modified gold nanoparticles
    Kubilay Güçlü
    Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, 34320 Istanbul, Turkey
    Anal Chim Acta 794:90-8. 2013
    ..Under optimized conditions, cysteine (Cys) was quantified by the proposed assay, with a detection limit (LOD) of 0.57μM and acceptable linearity ranging from 0.4 to 29.0μM (r=0.998). ..
  84. ncbi The properties of the metal-thiolate clusters in recombinant mouse metallothionein-4
    Bin Cai
    Chemical Biology Lab, Department of Chemistry, Fudan University, Shanghai, 200433, China
    Protein J 24:327-36. 2005
    ..But while reacting with EDTA, the metal-thiolate clusters in MT-4 are more stable than those of MT-1. We suppose the negative charge of the beta-domain of MT-4 prevents the EDTA attack to MT-4...
  85. ncbi Cellular disulfide-reducing capacity: an integrated measure of cell redox capacity
    James M May
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 344:1352-9. 2006
    ..Together, these results show that alpha-lipoic acid-dependent DTNB reduction provides a simple method to assess the disulfide-reducing capacity of intact cells, especially as determined by pyridine nucleotide-disulfide oxidoreductases...
  86. pmc Identification of a stable flavin-thiolate adduct in heterotetrameric sarcosine oxidase
    Robert M G Hynson
    Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA
    J Mol Biol 362:656-63. 2006
    ..A model of the postulated TSOX adduct suggests that it is stabilized by nearby residues that may be important in the electron transferase/oxidase function of the coenzyme...
  87. pmc Precision-cut tissue chips as an in vitro toxicology system
    J M Catania
    Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
    Toxicol In Vitro 21:956-61. 2007
    ..Importantly, the number of samples per organ/mouse was increased at least threefold and a significant time reduction per analysis was realized...
  88. ncbi Carboxymethylproline synthase (CarB), an unusual carbon-carbon bond-forming enzyme of the crotonase superfamily involved in carbapenem biosynthesis
    Mark C Sleeman
    Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK
    J Biol Chem 279:6730-6. 2004
    ..The implications of the results for the biosynthesis of both carbapenem-3-carboxylate and C-2/C-6-substituted carbapenems, such as thienamycin, are discussed...
  89. ncbi Contribution of cysteines to clathrin trimerization domain stability and mapping of light chain binding
    Joel A Ybe
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Traffic 4:850-6. 2003
    ..EMBO J 2002; 21: 6072-6082; Pishvaee B, Munn A, Payne GS. EMBO J 1997; 16: 2227-2239)...
  90. ncbi Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitors
    Oliver Rackham
    Western Australian Institute for Medical Research, Centre for Medical Research, University of Western Australia, Perth, WA 6000, Australia
    Free Radic Biol Med 50:689-99. 2011
    ..TrxR2 displayed strikingly lower activity with dithionitrobenzoic acid (DTNB), lipoamide, and the quinone substrate juglone compared to TrxR1, and TrxR2 could not reduce lipoic ..
  91. ncbi Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway
    Hang Mu
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
    Int J Biol Macromol 41:439-46. 2007
    ....
  92. ncbi Reduction of aggregation of β-lactoglobulin during heating by dihydrolipoic acid
    Heni B Wijayanti
    School of Agriculture and Food Sciences, The University of Queensland, Brisbane QLD 4072, Australia
    J Dairy Res 80:383-9. 2013
    ..However, the advantage of using DHLA over NEM and DTNB to lessen aggregation of β-Lg is that it is a food-grade compound which occurs naturally in milk. ..
  93. ncbi Intracellular adenosine triphosphate and glutathione concentrations in oocytes from first estrous, multi-estrous, and testosterone-treated gilts
    J R Herrick
    Department of Animal Sciences, The Ohio State University, 2027 Coffey Road, Columbus, OH 43210, USA
    Anim Reprod Sci 78:123-31. 2003
    ..Of the numerous potential markers of developmental potential, two were examined in the current study, and GSHi appeared to be useful for assessing porcine oocytes...
  94. ncbi A bicistronic expression system for bacterial production of authentic human interleukin-18
    Robert B Kirkpatrick
    Department of Gene Expression, Protein Biochemistry, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Rd, King of Prussia, PA 19406, USA
    Protein Expr Purif 27:279-92. 2003
    ....
  95. ncbi The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocket
    P L Brown
    Department of Cell Biology, Jewish Hospital, Washington University Medical Center, St Louis, MO 63110
    Biochem Biophys Res Commun 186:549-55. 1992
    ..The side chain of a conserved lysine residue in the disulfide-bonded loop forms the top of the pocket. This positively charged pocket may define a binding site for acidic microfibrillar proteins that mediate elastic fiber assembly...
  96. pmc A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity
    A J Lloyd
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Nucleic Acids Res 23:2886-92. 1995
    ..Finally, this novel method was used to provide direct evidence for the cooperativity of tRNA and ATP binding to GlnRS...
  97. ncbi Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate
    G A O'Toole
    Department of Bacteriology, University of Wisconsin, Madison 53706, USA
    J Biol Chem 270:23560-9. 1995
    ..In vitro experiments are consistent with previous genetic studies which had suggested that adenosyl-cobinamide was the preferred substrate of CobU, and that CobU functioned more efficiently in the absence of oxygen...
  98. ncbi Effect of lecithin:cholesterol acyltransferase on distribution of apolipoprotein A-IV among lipoproteins of human plasma
    C L Bisgaier
    J Lipid Res 28:693-703. 1987
    ..ApoA-IV may bind to these "gaps," where the packing density of the phospholipid head groups is reduced...
  99. ncbi Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kinetics
    K Mino
    Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan
    Biosci Biotechnol Biochem 64:1628-40. 2000
    ....
  100. ncbi Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman's reagent and monobromobimane
    F E Sneller
    Department of Ecology and Ecotoxicology, Vrije Universiteit Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Agric Food Chem 48:4014-9. 2000
    ..4% and 50.2% for PC(4). This decrease is most likely due to steric hindrance. Correction of measured thiol concentration is therefore advised for better quantification of PC concentrations in plant material...
  101. ncbi Chemical modification of xylanases: evidence for essential tryptophan and cysteine residues at the active site
    V Deshpande
    Division of Biochemical Sciences, National Chemical Laboratory, Pune, India
    Biochim Biophys Acta 1041:172-7. 1990
    ..Xylan afforded complete protection against inactivation by NBS, HNBB and PHMB, indicating the involvement of tryptophan and cysteine residues at the substrate-binding region of the enzyme...

Research Grants12

  1. Calorimetry Work Station
    SHERWIN LEHRER; Fiscal Year: 2002
    ..iii) measure binding constants and characterize the thermodynamics of binding equilibria. ITC avoids the unavoidable perturbation of fluorescence labels and is more quantitatively than ELISA assays of binding. ..
  2. TROPOMYOSIN AND THE REGULATION OF MUSCLE CONTRACTION
    SHERWIN LEHRER; Fiscal Year: 2009
    ..These measurements between specific labeled amino acid residues will provide information about the dynamics of the changes between the components involved in the regulatory process. ..
  3. Anesthetics block neuronal voltage-gated Ca2+ currents
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..abstract_text> ..
  4. ABCA1 agonist peptides for therapeutic use
    JOHN BIELICKI; Fiscal Year: 2008
    ..The research is likely to have a high impact on public health, since the therapeutics will be amenable to the widespread treatment of atherosclerosis. [unreadable] [unreadable] [unreadable] [unreadable]..
  5. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  6. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  7. ApoA-I Helical Domains and Cardiovascular Aging
    JOHN BIELICKI; Fiscal Year: 2005
    ..These studies are relevant for devising novel strategies to combat cardiovascular aging. ..
  8. Acetylcholinesterase: A Comparative Biochemical Approach
    Leo Pezzementi; Fiscal Year: 2005
    ..abstract_text> ..
  9. MULTIPLE CLASSES OF CA2+ CHANNELS - SENSORY TRANSMISSION
    SLOBODAN TODOROVIC; Fiscal Year: 2004
    ..abstract_text> ..
  10. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
  11. PRESERVATION OF HDL FUNCTION DURING EARLY ATHEROGENESIS
    JOHN BIELICKI; Fiscal Year: 2002
    ..Moreover, the research will uncover novel mechanisms for preserving HDL/LCAT function that can be utilized therapeutically to fight the onset of cardiovascular disease. ..
  12. REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONS
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..