Genomes and Genes
Summary: A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.
Publications124 found, 100 shown here
- Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interactionH J Ryser
Department of Pathology, Boston University School of Medicine, MA 02118
Proc Natl Acad Sci U S A 91:4559-63. 1994..These findings suggest additional approaches to impede cell infection by HIV...
- The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell bindingE Fenouillet
Centre National de la Recherche Scientifique, Faculte de Medecine Nord, Marseille, France
J Infect Dis 183:744-52. 2001..The fact that the PDI catalytic activity present on lymphocytes is required for fusion supports the hypothesis that catalysts assist post-CD4 cell binding conformational changes within Env...
- Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entryUthaman Gowthaman
Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
BMC Bioinformatics 9:S14. 2008..This study attempts to perceive the mode of binding of dithionitrobenzoic acid (DTNB), and its structurally related compounds on PDI enzyme.
- Simple shifts in redox/thiol balance that perturb blood coagulationHenry K Bayele
Department of Haematology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
FEBS Lett 510:67-70. 2002..These data also suggest that blood clotting may be influenced by unidentified redox or thiol equilibria...
- Changes in sulfhydryl content of egg white proteins due to heat and pressure treatmentIesel Van der Plancken
Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Heverlee, Belgium
J Agric Food Chem 53:5726-33. 2005..Treatment conditions resulting in extensive protein insolubilization were accompanied by a transfer of free sulfhydryl groups from the soluble to the insoluble protein fraction. These SH groups were mainly accessible to DTNB...
- Structural and functional studies of vertebrate metallothioneins: cross-talk between domains in the absence of physical contactClemente Capasso
CNR, Institute of Protein Biochemistry, via P. Castellino 111 I 80131 Naples, Italy
Biochem J 391:95-103. 2005..Taken together, these results provide a strong argument in favour of the interaction of the two domains in the MT molecule, in spite of the elusive evidence provided by the structural analyses...
- Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bondsJane E Raulston
Department of Microbiology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0579, USA
Infect Immun 70:535-43. 2002..The accessibility of the Hsp70 substrate-binding domain may support the hypothesis that this protein plays a role in events following the initial stage of attachment instead of serving as a primary, surface-displayed adhesin...
- Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagentsS J McCormick
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
Acta Biochim Pol 48:573-8. 2001..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
- Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1J Y Hwang
, , Germany
FEBS Lett 508:355-9. 2001..9 microM for Ca(2+) binding to this site with a fast off-rate (t approximately 2 ms). We conclude that the rapid dissociation of Ca(2+) from EF-hand 3 in GCAP-1 triggers activation of guanylate cyclase in rod cells...
- The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid)Zhao Feng
Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
Int J Biol Macromol 36:184-90. 2005..The reactive cysteine of AK, which was proposed to be Cys274, may play an important role not in the binding of the transition-state analog but in the conformational changes caused by the transition-state analog...
- Cleavage of disulfide-linked fetuin-bisphosphonate conjugates with three physiological thiolsSufeng Zhang
Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB T6G 2G6, Canada
Biomacromolecules 6:2800-8. 2005..In conclusion, disulfide-linked fetuin-thiolBP conjugates exhibited a high affinity to HA, which was readily lost upon cleavage with thiols found in physiological milieu...
- Modifications of cysteine residues in the solution and membrane-associated conformations of phosphatidylinositol transfer protein have differential effects on lipid transfer activityJ M Tremblay
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
Biochemistry 40:9151-8. 2001..Furthermore, the inhibition of PITP activity by thiol-modifying reagents is a result of steric hindrance of phospholipid substrate binding...
- Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple formKehinde O Okonjo
Department of Chemistry, University of Ibadan, Ibadan, Nigeria
Biophys Chem 119:196-204. 2006..Analyses of the simple profiles gave pKas of 6.33 +/- 0.17 and 8.54 +/- 0.5 for HisH21beta and other histidines within a distance of 2 nm of the sulfhydryl, and CysF9beta of the minor hemoglobin, respectively...
- Critical flocculation concentrations, binding isotherms, and ligand exchange properties of peptide-modified gold nanoparticles studied by UV-visible, fluorescence, and time-correlated single photon counting spectroscopiesHuan Xie
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
Anal Chem 75:5797-805. 2003..Full characterization of the composition and stability of peptide-modified metal nanoparticles is an important step in their use as intracellular delivery vectors and imaging agents...
- Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activityJ Lee
Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
J Biol Chem 276:14804-13. 2001..Taken together, these results demonstrate that both histidines assume critical roles for catalysis...
- Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase--a potential target for antimicrobial agents against Pseudomonas aeruginosaRoberto Velasco-García
Laboratorio de Osmorregulación, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Avenida de Los Barrios, Tlalnepantla, Estado de Mexico, 54090 Mexico, Mexico
Biochem Biophys Res Commun 341:408-15. 2006..methyl-methanethiosulfonate inactivated the enzymes without affecting their stability while the bulkier dithionitrobenzoic acid (DTNB) and bis[diethylthiocarbamyl] disulfide (disulfiram) induced enzyme dissociation--at 23 degrees C--..
- Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9beta sulfhydryl groups of the hemoglobins of the domestic cat: variation of the equilibrium and reverse rate constants with pHKehinde Onwochei Okonjo
Department of Chemistry, University of Ibadan, Ibadan, Nigeria
Biophys Chem 121:65-73. 2006..2+/-0.2 and 9.4+/-0.1 in the major hemoglobin and 6.7+/-0.3 and 8.4+/-0.1 in the minor hemoglobin...
- Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implicationsR Rossi
Department of Neuroscience, Pharmacology Unit, Via A. Moro 4, University of Siena, Italy
J Biol Chem 276:7004-10. 2001..This is thought to influence significantly the biochemical, pharmacological, and toxicological effects of some drugs...
- On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobinZ Feng
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA
J Mol Biol 314:153-66. 2001..The C73 thiol group is exposed by a low-energy fluctuation that does not expose its amide group to exchange...
- Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediatesK Sridevi
National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bangalore, India 560065
Biochemistry 41:1568-78. 2002....
- Identification and characterization of a critical region in the glycogen synthase from Escherichia coliAlejandra Yep
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
J Biol Chem 279:8359-67. 2004..The results of this work indicate that this region is critical for catalysis and substrate binding...
- Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liverTung Kung Wu
Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
Arch Biochem Biophys 421:42-53. 2004..The stoichiometry of histidine modification and the extent of inactivation showed that two essential histidine residues per active site are necessary for complete bovine liver OSC activity...
- Implications of the role of reactive cystein in arginine kinase: reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified arginine kinase reactivated by dithiothreitolJi-Cheng Pan
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, PR China
Biochem Biophys Res Commun 317:539-44. 2004..The reactive cysteine of arginine kinase may play an important role not in the binding to the transition-state analog but in the conformational changes caused by the transition-state analog...
- Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanismIsabella Dalle-Donne
Department of Biology, University of Milan, Milan, Italy
Free Radic Biol Med 35:1185-93. 2003....
- Roles for cysteine residues in the regulatory CXXC motif of human mitochondrial branched chain aminotransferase enzymeMyra E Conway
Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, North Carolina 27157, USA
Biochemistry 43:7356-64. 2004..Thus, C315 appears to be the sensor for redox regulation of BCAT activity, whereas C318 acts as the "resolving cysteine", allowing for reversible formation of a disulfide bond...
- Antiviral activity of RhoA-derived peptides against respiratory syncytial virus is dependent on formation of peptide dimersPhilip J Budge
Department of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Antimicrob Agents Chemother 47:3470-7. 2003....
- Development and application of serum cholinesterase activity measurement using benzoylthiocholine iodideSusumu Osawa
School of Medicine, Biological Sciences and Technology, Department of Health Sciences, Kyushu University, Higashi, Fukuoka City 812 8582, Japan
Clin Chim Acta 351:65-72. 2005..Many procedures using various artificial substrates have been developed but suffer from problems with substrate specificity and interference by endogenous substances...
- Single-molecule covalent chemistry with spatially separated reactantsTudor Luchian
Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843-1114, USA
Angew Chem Int Ed Engl 42:3766-71. 2003
- Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1Judith Lahav
The Coagulation Laboratory, Rabin Medical Center Beilinson Campus, Zabotinski St, Petah Tiqva 49100, Israel
Blood 102:2085-92. 2003..These data suggest that the enzymatically catalyzed adhesion-associated reorganization of disulfide bonds is common to members of the integrin family and specific to this family...
- Kinetic studies of rat liver hexokinase D ('glucokinase') in non-co-operative conditions show an ordered mechanism with MgADP as the last product to be releasedOctavio Monasterio
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Biochem J 371:29-38. 2003..In addition, the straight-line dependence observed when the reciprocal value of the inactivation constant was plotted against the sugar-ligand concentration supports the view that there is just one sugar-binding site in hexokinase D...
- Perturbation of microtubule polymerization by quercetin through tubulin binding: a novel mechanism of its antiproliferative activityKamlesh Gupta
BJM School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India
Biochemistry 41:13029-38. 2002..The data suggest that quercetin inhibits cancer cells proliferation at least in part by perturbing microtubule functions through tubulin binding...
- The roles of thiols in the bacterial organomercurial lyase (MerB)Keith E Pitts
Department of Microbiology and Center for Metalloenzyme Studies, The University of Georgia, Athens, Georgia 30602, USA
Biochemistry 41:10287-96. 2002..On the basis of the foregoing we refine the previously proposed S(E)2 mechanism for protonolysis by MerB...
- Role of cysteine 306 in the catalytic mechanism of Ascaris suum phosphoenolpyruvate carboxykinaseSandra E Rios
Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA
Arch Biochem Biophys 404:25-37. 2002..Alternatively, mutation of this residue could cause protein changes that interfere with the proper conformation of the nucleotides for optimal catalysis to take place...
- Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cellsC H Davis
Department of Microbiology and Immunology, School of Medicine, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA
Infect Immun 70:3413-8. 2002..trachomatis serovar E entry...
- Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerizationP J Britto
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Biochem J 389:549-58. 2005..Under such conditions, the substituted tubulin forms protofilament-based structures such as microtubules, open tubules, sheets and/or bundles...
- Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: an example of application for protein chemistryGo Hasegawa
Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
J Biochem Biophys Methods 63:33-42. 2005..These data showed that IADC is a useful reagent to specifically identify the positions of a Cys residue(s) in proteins...
- Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liverHao Chen
Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22, 14195 Berlin-Dahlem, Germany
Glycobiology 12:65-71. 2002....
- Reduced redox state allows prolonged survival of axotomized neonatal retinal ganglion cellsL K Geiger
Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, 600 Highland Avenue, Madison, WI 53792, USA
Neuroscience 109:635-42. 2002..Understanding the mechanisms by which ROS signal neuronal death could result in strategies for increasing their long-term survival after axonal injury...
- The role of cysteine residues in tellurite resistance mediated by the TehAB determinantM Dyllick-Brenzinger
Structural Biology Research Group, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
Biochem Biophys Res Commun 277:394-400. 2000..Studies using native and SDS denaturing PAGE under reducing and oxidizing conditions suggested that a cysteine in TehB is involved in binding tellurite...
- Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli WM Iwakura
J Biochem 86:1239-49. 1979..The protective effect of NADP+ was increased by the addition of Mg2+. Based on the above results, the effects of Mg2+ on the SH-group modification are discussed from the viewpoint of conformational alteration of the enzyme...
- Characterization of chitosan thiolation and application to thiol quantification onto nanoparticle surfaceI Bravo Osuna
Laboratoire de Physicochimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Universite Paris Sud 11, Faculte de Pharmacie, 5, rue J B Clement, 92296 Chatenay Malabry, France
Int J Pharm 340:173-81. 2007..It was successfully applied to the quantification of active thiol groups on the surface of poly(isobutyl cyanoacrylates) nanoparticles coated with thiol chitosan...
- Role of cysteine residues in 4-oxalomesaconate hydratase from Pseudomonas ochraceae NGJ1Suhong Li
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
Biosci Biotechnol Biochem 71:449-57. 2007....
- Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase systemThomas J Jönsson
Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
Biochemistry 46:5709-21. 2007..In addition, two new constructs have been generated, an AhpF-AhpC complex and a "twisted" form of AhpF, in which redox centers are locked together by stable disulfide bonds which mimic catalytic intermediates...
- Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytesG Gao
Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
J Biol Chem 269:8234-9. 1994..Residues 5-12 and 15 of this peptide are identical with a sequence of a yeast alkaline p-nitrophenylphosphatase, and the peptide has little homology with other mammalian phosphatases...
- Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinaseHua Wei He
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
Int J Biol Macromol 41:361-8. 2007..Furthermore, the findings in this study may provide a novel explanation for the low pKa value of Cys283...
- Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coliS R Dowd
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
Biochim Biophys Acta 1252:278-83. 1995..Cysteine 492 has been identified as containing the sulfhydryl that is accessible to the reagent under both conditions...
- Recombinant lysine:N(6)-hydroxylase: effect of cysteine-->alanine replacements on structural integrity and catalytic competenceScott Dick
Department of Chemistry, University of Waterloo, ON, Canada
Biochim Biophys Acta 1594:219-33. 2002..A possible role of rIucD's Cys51 in the modulation of its mono-oxygenase function is discussed...
- Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coliM M Altamirano
Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, D F
Arch Biochem Biophys 258:95-100. 1987..The results obtained show that sulfhydryls are not essential for catalysis or allosteric behavior of glucosamine-6-phosphate deaminase...
- A bicistronic expression system for bacterial production of authentic human interleukin-18Robert B Kirkpatrick
Department of Gene Expression, Protein Biochemistry, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Rd, King of Prussia, PA 19406, USA
Protein Expr Purif 27:279-92. 2003....
- Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathwayHang Mu
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
Int J Biol Macromol 41:439-46. 2007....
- Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active siteWen Jing Zhu
College of Life Science, Shandong Agricultural University, Shandong Taian 271018, People s Republic of China
Int J Biol Macromol 41:564-71. 2007..Using the specific chemical modifier phenylglyoxal (PG) demonstrated that only one Arg, located near the ATP binding site, is essential for the activity of AK...
- Contribution of redox status to hepatitis C virus E2 envelope protein function and antigenicityEmmanuel Fenouillet
UMR 6184, CNRS, Université Aix Marseille II, F 13015, France
J Biol Chem 283:26340-8. 2008..Although E2 function is surprisingly independent of its redox status, its disulfide bonds mask antigenic domains. E2 redox manipulation may contribute to improved vaccine design...
- Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coliM Yamaguchi
J Biochem 86:325-33. 1979..Based on the above results, the mechanism of the alteration of enzyme activities by sulfhydryl group modification is discussed...
- A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGKMatthew J Cliff
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
J Mol Biol 364:810-23. 2006..The success of the competition technique in characterizing this kinetic intermediate invites application to other model systems...
- Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesisO Bjornberg
Center for Enzyme Research, Institute of Molecular Biology, and Centre for Crystallograpic Studies, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
Biochemistry 36:16197-205. 1997..The possible acid-base chemistry of Cys 130 is compared to previous work on mammalian dihydropyrimidine dehydrogenases, flavoenzymes, which catalyze the reversed reaction, namely the reduction of pyrimidine bases...
- Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagentR Daniel
Laboratoire de Bioorganique et Biotechnologies associé au Centre National de la Recherche Scientifique, Ecole Nationale Superieure de Chimie de Paris, 11 rue P and M Curie, 75231 Paris Cedex 05, France
J Biol Chem 272:26934-9. 1997..Consistent with literature data, we assume that cysteine 221 and cysteine 265, along with the neighboring cysteines 263 and 268 arranged in cluster, are potential ligands of structural zinc of E. coli dihydroorotase...
- Zinc transfer potentials of the alpha - and beta-clusters of metallothionein are affected by domain interactions in the whole moleculeL J Jiang
Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Seeley G Mudd Building, 250 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 97:2503-8. 2000..The two-domain structure of the whole molecule is important for its interaction with ligands and for control of its reactivity and overall conformation...
- Regulation of PTP1B via glutathionylation of the active site cysteine 215W C Barrett
Laboratory of Biochemistry, Section of Metabolic Regulation, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892, USA
Biochemistry 38:6699-705. 1999..Glutathionylation of the cysteine sulfenic derivative will prevent the enzyme from further oxidation to its irreversible forms, and constitutes an efficient regulatory mechanism...
- Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid)M J Nozal
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain
J Chromatogr A 778:347-53. 1997..The detection limits are 0.21, 0.92 and 0.61 mumol/g wet sample for glutathione, cysteine and N-acetylcysteine, respectively...
- Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistryArlin B Rogers
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Lab Invest 86:526-33. 2006..These findings provide a molecular basis to improve the specificity of IHC and other immunoassays, and hold implications for antibody-based clinical diagnostics and therapeutics...
- Characterization of a mammalian peroxiredoxin that contains one conserved cysteineS W Kang
Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 273:6303-11. 1998..Thus, the primary cellular function of 1-Cys Prx appears to be to reduce peroxides with the use of electrons provided by an as yet unidentified source; the enzyme therefore represents a new type of peroxidase...
- DNA damage, apoptosis and cell cycle changes induced by fluoride in rat oral mucosal cells and hepatocytesLing Fei He
Department of Dental Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
World J Gastroenterol 12:1144-8. 2006..To study the effect of fluoride on oxidative stress, DNA damage and apoptosis as well as cell cycle of rat oral mucosal cells and hepatocytes...
- Comparative inhibition by hard and soft metal ions of steroid-binding capacity of renal mineralocorticoid receptor cross-linked to the 90-kDa heat-shock protein heterocomplexM D Galigniana
Departamento de Qu approximately ímica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, and PHROM CONICET, 1428 Buenos Aires, Argentina
Biochem J 341:585-92. 1999..role played by these residues in steroid binding was confirmed by chemical modification of cysteines with dithionitrobenzoic acid, histidines with diethyl pyrocarbonate and acidic amino acids with Woodward's reagent (N-ethyl-5-..
- Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stagesMin Kyu Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
J Vet Med Sci 69:627-32. 2007..GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay...
- Irreversible activation of cyclic nucleotide-gated ion channels by sulfhydryl-reactive derivatives of cyclic GMPR L Brown
Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, Oregon 97209, USA
Biochemistry 39:432-41. 2000..Pretreatment of the patch with the sulfhydryl-blocking reagents N-ethylmaleimide (NEM) and bis-dithionitrobenzoic acid (DTNB) prevented covalent activation; the effect of DTNB was reversed by reduction with DTT...
- Selenium in thioredoxin reductase: a mechanistic perspectiveBrian M Lacey
Department of Biochemistry, College of Medicine, University of Vermont, 89 Beaumont Avenue, Given Building Room B413, Burlington, Vermont 05405, USA
Biochemistry 47:12810-21. 2008..Further study of the biochemical properties of the truncated Cys and Sec TR enzymes demonstrates that the chemical advantage conferred on the eukaryotic enzyme by a selenol is the ability to function at acidic pH...
- Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acidWei Chen
Department of Pharmaceutical Sciences, College of Pharmacy, South Dakota State University, Brookings, SD 57007, United States
J Pharm Biomed Anal 48:1375-80. 2008..This method should be applicable to cellular, subcellular, protein, or other biomatrix samples for thiol and disulfide quantification and will be a useful analytical method in the study of thiol redox state and thiol glutathionylation...
- Pyruvate dehydrogenase kinase isoform 2 activity stimulated by speeding up the rate of dissociation of ADPHaiying Bao
Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
Biochemistry 43:13442-51. 2004..We suggest that passing even limited reducing equivalents among lipoyl groups maintains E2 lipoyl domains in a conformation that aids kinase function...
- A visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthaseD Andrew Skaff
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
Anal Biochem 396:96-102. 2010..The high-throughput assay method has been successfully used to screen a library of more than 100,000 drug-like compounds and has identified both reversible and irreversible inhibitors of the human enzyme...
- Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarumChun You
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
Biotechnol Bioeng 105:861-70. 2010..at sites 50 and 201, rather than the disulfide bond formation which was ruled out by thiol titration with dithionitrobenzoic acid (DTNB)...
- Cholesteryl ester transfer protein promotes the formation of cholesterol-rich remnant like lipoprotein particles in human plasmaHiroshi Okamoto
Central Pharmaceutical Research Institute, Japan Tobacco Inc, Takatsuki, Osaka, 569 1125, Japan
Clin Chim Acta 375:92-8. 2007..Cholesteryl ester transfer protein (CETP) is suggested to be involved in the cholesterol level in remnant like lipoprotein particles (RLP), but there is no direct evidence that CETP increases cholesterol-rich RLP in plasma...
- A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57Diego A Ellerman
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Davis, California 95616, USA
Dev Cell 10:831-7. 2006..The results support the idea that thiol-disulfide exchange is a mechanism that may act in gamete fusion to produce conformational changes in fusion-active proteins...
- Sublethal concentrations of diverse gold compounds inhibit mammalian cytosolic thioredoxin reductase (TrxR1)Yo Omata
Hokkaido University, Sapporo, Japan
Toxicol In Vitro 20:882-90. 2006..Inhibition may be optimized to some degree by altering the ligand configuration of the compounds. These results support future study of a variety of Au compounds for therapeutic development as inhibitors of TrxR1...
- Simultaneous enzymatic kinetic determination of pesticides, carbaryl and phoxim, with the aid of chemometricsYongnian Ni
Department of Chemistry, Nanchang University, Nanchang 330047, China
Anal Chim Acta 588:131-9. 2007..2; phoxim=6.5), %Recovery (approx.105%) and %RPE(T) (6.5). Various spiked town-water samples produced recoveries in the range of 98.8-103% for each pesticide...
- Kinetic intermediates of unfolding of dimeric prostatic phosphataseRadosława Kuciel
Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Krakow, Poland
Acta Biochim Pol 54:371-7. 2007..Together with previous studies these results suggest that the stability of the catalytically active conformation of the enzyme depends mainly on the dimeric structure of the native hPAP...
- A colorimetric assay for S-adenosylhomocysteine hydrolaseJ D Lozada-Ramírez
Department of Biochemistry and Molecular Biology A, Faculty of Biology, University of Murcia, Campus Espinardo, E 30071 Murcia, Spain
J Biochem Biophys Methods 67:131-40. 2006..This method is an easy, cheap and shorter alternative to more complex methods and it is applicable to routine clinical analysis and in the assay and development of new S-nucleosidylhomocysteines to be used as therapeutic compounds...
- Fatty acyl-CoA as an endogenous activator of UDP-glucuronosyltransferasesKazuharu Okamura
Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan
Biochem Biophys Res Commun 345:1649-56. 2006..These results suggest that: (1) acyl-CoAs play a role as an endogenous activator of UGTs, and (2) a sulfhydryl group is required for the activation of UGT by physiological concentrations of acyl-CoAs...
- Metal binding ability of metallothionein-3 expressed in Escherichia coliShigeru Toriumi
Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Nishi-5, Sapporo 060-0810, Japan
Basic Clin Pharmacol Toxicol 96:295-301. 2005..However, the affinity of metallothionein-3 to Cu was much higher than that of metallothionein-1 and -2. The strong affinity to Cu of metallothionein-3 might be related to its growth inhibitory activity...
- Photodegradation mechanism and reaction kinetics of recombinant human interferon-alpha2aHyong Ha Kim
Health Metrology Group, Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, P O Box 102, Yuseong, Daejeon 305 600, Korea
Photochem Photobiol Sci 6:171-80. 2007..The relationship between the protein conformation and photobleaching rate could be explained based on the competition between excited state energy transfer and the photoionization process in Trp residues...
- Escherichia coli cyclopropane fatty acid synthaseFabienne Courtois
Laboratoire de Chimie Organique Biologique, UMR7613 CNRS, Universite Pierre et Marie Curie, Paris, France
Eur J Biochem 271:4769-78. 2004..The inactivation is probably the result of steric hindrance, a phenomenon irrelevant to catalysis. It is very likely that E. coli CFAS operates via a carbocation mechanism, but the base and nucleophile remain to be identified...
- NAP1 catalyzes the formation of either positive or negative supercoils on DNA on basis of the dimer-tetramer equilibrium of histones H3/H4Sharon Peterson
Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
Biochemistry 46:8634-46. 2007..It is proposed that these conformational changes in H3/H4 are involved in promoter activation and transcription elongation through nucleosomes...
- Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplusJorge Moraes
Laboratório de Química e Função de Proteínas e Peptídeos and Unidade de Experimentação Animal RJ, CBB, UENF, Avenida Alberto Lamego 2000, Horto, Campos dos Goytacazes, RJ, CEP 28015 620, Brazil
Insect Biochem Mol Biol 41:400-9. 2011..Furthermore, BmTIM was highly sensitive to the action of the thiol reagents dithionitrobenzoic acid and methyl methane thiosulfonate, suggesting that there are five cysteines exposed in each dimer and that ..
- The thiol group of the L-arabinose-binding protein. Chromophoric labeling and chemical identification of the sugar-binding siteD M Miller
J Biol Chem 254:7521-8. 1979
- Interaction of two sulfhydryl reagents with a cation recognition site on the neuronal dopamine carrier evidences small differences between [3H]GBR 12783 and [3H]cocaine binding sitesF Refahi-Lyamani
EP 076 du C N R S, U F R de Médecine and Pharmacie de Rouen, Saint Etienne du Rouvray, France
Naunyn Schmiedebergs Arch Pharmacol 351:136-45. 1995..When striatal cell membranes were treated in a medium containing 130 mmol/l Na+, there was a general decrease in the effects of ions on the reductions of specific binding produced by DTNB or pHMBS.(ABSTRACT TRUNCATED AT 400 WORDS)..
- Inhibition studies on calf pregastric esterase: the enzyme has no functional thiol groupM Y Timmermans
Department of Biochemistry, Limburgs Universitair Centrum, Diepenbeek, Belgium
Biochem J 314:931-6. 1996..The fact that PGE is not inhibited by thiol reagents indicates that PGE has no functional free thiol group. The PGE cDNA codes only for two cysteines, and their involvement in the formation of a disulphide bridge was demonstrated...
- Characterization of the denaturation and renaturation of human plasma vitronectin. II. Investigation into the mechanism of formation of multimersP Zhuang
Department of Biochemistry, University of Tennessee, Knoxville, Tennessee 37996, USA
J Biol Chem 271:14333-43. 1996....
- Absolute on-line molecular mass analysis of basic fibroblast growth factor and its multimers by reversed-phase liquid chromatography with multi-angle laser light scattering detectionI V Astafieva
Wyatt Technology Corporation, Santa Barbara, CA 93103, USA
J Chromatogr A 740:215-29. 1996....
- Characteristics of rabbit muscle adenylate kinase inhibition by sulfur and recovery by dithiothreitolP J Russell
Department of Biology, University of California, San Diego, La Jolla 92093, USA
J Enzyme Inhib 9:179-94. 1995..The results of these studies show that the activity of a small enzyme can be controlled in a manner analogous to the allosteric control of larger enzymes...
- Structural characterization of the metal binding site in the cysteine-rich region of HIV-1 Tat proteinH W Huang
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China
Biochem Biophys Res Commun 227:615-21. 1996..Five out of seven Cys residues are coordinating with two Zn cations of the complex Zn-Tat21-38 (2:1). A model of the coordination arrangement of Zn binding sites at different states is proposed...
- The sulfhydryl groups of Cys 269 and Cys 272 are critical for the oligomeric state of chloroplast carbonic anhydrase from Pisum sativumH Bjorkbacka
Department of Biochemistry, Umea University, Sweden
Biochemistry 36:4287-94. 1997..Furthermore, the data demonstrate that C269 and C272 are involved in the interaction between subunits and are necessary for a proper structure at the tetramer-tetramer interface...
- Contrasting effects of intracellular redox couples on the regulation of maxi-K channels in isolated myocytes from rabbit pulmonary arteryD Thuringer
CNRS UMR 6542, Faculte des Sciences et Techniques, Universite de Tours, France
J Physiol 500:583-92. 1997..The sulfhydryl-specific redox reagents (DTNB and BME) must act through another regulatory mechanism...
- Identification of the fifth axial heme ligand of chloroperoxidaseS R Blanke
Department of Biochemistry, University of Illinois, Urbana 61801
J Biol Chem 263:18739-43. 1988..Proteolysis and isolation of the labeled peptides using reverse phase HPLC and subsequent Edman sequence analysis detected and identified the thionitrobenzoate adducts of each of the three cysteinyl peptides of chloroperoxidase...
- Role of the essential thiol group in the thiol-activated cytolysin from Clostridium perfringensM Iwamoto
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan
Eur J Biochem 167:425-30. 1987....
- Reactivities of sulfhydryl groups in native and metal-free aminoacylase ID Heese
Institut fur Physiologische Chemie, Universitat Marburg
Biol Chem Hoppe Seyler 370:607-12. 1989..It appears that binding of the inhibitor or removal of the metal ion induces conformational changes of the amino-acylase active site that render a buried sulfhydryl group more accessible to modification...
- Velocity of Ellman's reaction and its implication for kinetic studies in the millisecond time rangeJ Stojan
Institute of Biochemistry, Medical Faculty, Ljubljana, Slovenia
Neurochem Res 17:1207-10. 1992....
- Abnormal fibrinogens IJmuiden (B beta Arg14----Cys) and Nijmegen (B beta Arg44----Cys) form disulfide-linked fibrinogen-albumin complexesJ Koopman
IVVO TNO, Gaubius Laboratory, Leiden, The Netherlands
Proc Natl Acad Sci U S A 89:3478-82. 1992..We also found that a significant fraction of the abnormal fibrinogen molecules contained free sulfhydryl groups. These findings complicate interpretation of functional studies of these altered fibrinogens...
- Immunological assay of erythrocyte acetylcholinesteraseJ W Jones
Blood Transfusion Department, Torbay Hospital, Plymouth, UK
Clin Chim Acta 200:175-81. 1991..The coefficient of variation for each method is 7%. Reference ranges have been established for healthy adults and cord blood...
- Thermostabilization of recombinant human and bovine CuZn superoxide dismutases by replacement of free cysteinesR A Hallewell
Chiron Corporation, Emeryville, CA 94608
Biochem Biophys Res Commun 181:474-80. 1991..In all cases, removal of a free cysteine residue increased thermostability...
- Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modificationS Kanaya
Protein Engineering Research Institute, Osaka, Japan
Biochem J 271:59-66. 1990..Thus the sensitivity of E. coli RNAase H to N-ethylmaleimide arises not from blocking of the thiol group but from steric hindrance by the modifying group incorporated at either Cys-13 or Cys-133...
- Crystallization and structure solution at 4 A resolution of the recombinant synthase domain of N-(5'-phosphoribosyl)anthranilate isomerase:indole-3-glycerol-phosphate synthase from Escherichia coli complexed to a substrate analogueM Wilmanns
Department of Structural Biology, University of Basel, Switzerland
Protein Eng 3:173-80. 1990..This system provides a model for an interface which is used in both intermolecular and intramolecular domain contacts...
- Organization of functional groups of liver bilitranslocaseS Passamonti
Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Universita degli Studi di Trieste, Italy
Biochim Biophys Acta 1041:195-200. 1990..On the contrary, the other class of sulfhydryl groups can be derivatized, regardless of the state of the arginine residues...
- REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONSSLOBODAN TODOROVIC; Fiscal Year: 2009..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..
- ABCA1 agonist peptides for therapeutic useJOHN BIELICKI; Fiscal Year: 2007..The research is likely to have a high impact on public health, since the therapeutics will be amenable to the widespread treatment of atherosclerosis. ..
- PEROXYNITRITE DAMAGE TO MICROTUBULE PROTEINSLISA LANDINO; Fiscal Year: 2007..Researchers must consider and assess the effects of reactive molecules on abundant brain proteins because it is very likely that many proteins are damaged as we age. ..
- RESEARCH TRAINING IN DIABETES AND ENDOCRINOLOGYJames May; Fiscal Year: 2007..In the 31 years of NIH support, the program has supported 96 trainees, and over 70% have chosen careers in academia, industry, or government. ..
- ASCORBIC ACID FUNCTION AND METABOLISMJames May; Fiscal Year: 2007..By focusing on mechanisms in cell models and on early disease in animal models, this project will show whether and how vitamin C prevents atherosclerosis. ..
- Antioxidant Interaction of Selenium and Vitamins C & EJames May; Fiscal Year: 2007..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
- Antioxidant vitamins in models of Alzheimer's DiseaseJames May; Fiscal Year: 2007..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
- Vitamin C Transporters in the BrainJames M May; Fiscal Year: 2010..Several neurodegenerative diseases including Alzheimer's and Parkinson's are associated with increased oxidant stress so that this project will help define the role of vitamin C in oxidant injury to the brain. ..
- ASCORBIC ACID FUNCTION AND METABOLISMJames M May; Fiscal Year: 2010..By focusing on mechanisms in cell models and on early disease in animal models, this project will show whether and how vitamin C prevents atherosclerosis. ..
- REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONSSLOBODAN TODOROVIC; Fiscal Year: 2009..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..
- Anesthetics block neuronal voltage-gated Ca2+ currentsSLOBODAN TODOROVIC; Fiscal Year: 2009..abstract_text> ..
- ASCORBIC ACID FUNCTION AND METABOLISMJames May; Fiscal Year: 1999..We will also test the hypothesis that ascorbate-dependent recycling of alpha tocopherol in erythrocytes can replenish depleted stores of alpha tocopherol in human LDL. ..
- ANTIOXIDANT INTERACTIONS OF SELENIUM AND VITAMINSJames May; Fiscal Year: 2002....
- PRESERVATION OF HDL FUNCTION DURING EARLY ATHEROGENESISJOHN BIELICKI; Fiscal Year: 2002..Moreover, the research will uncover novel mechanisms for preserving HDL/LCAT function that can be utilized therapeutically to fight the onset of cardiovascular disease. ..
- ApoA-I Helical Domains and Cardiovascular AgingJOHN BIELICKI; Fiscal Year: 2005..These studies are relevant for devising novel strategies to combat cardiovascular aging. ..
- Acetylcholinesterase: A Comparative Biochemical ApproachLeo Pezzementi; Fiscal Year: 2005..abstract_text> ..
- ASCORBIC ACID FUNCTION AND METABOLISMJames May; Fiscal Year: 2004....
- MULTIPLE CLASSES OF CA2+ CHANNELS - SENSORY TRANSMISSIONSLOBODAN TODOROVIC; Fiscal Year: 2004..abstract_text> ..
- Antioxidant Functions of Lipoic AcidJames May; Fiscal Year: 2004..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..