dithionitrobenzoic acid

Summary

Summary: A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.

Top Publications

  1. ncbi Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction
    H J Ryser
    Department of Pathology, Boston University School of Medicine, MA 02118
    Proc Natl Acad Sci U S A 91:4559-63. 1994
  2. ncbi The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell binding
    E Fenouillet
    Centre National de la Recherche Scientifique, Faculte de Medecine Nord, Marseille, France
    J Infect Dis 183:744-52. 2001
  3. ncbi Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entry
    Uthaman Gowthaman
    Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
    BMC Bioinformatics 9:S14. 2008
  4. ncbi Simple shifts in redox/thiol balance that perturb blood coagulation
    Henry K Bayele
    Department of Haematology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
    FEBS Lett 510:67-70. 2002
  5. ncbi Changes in sulfhydryl content of egg white proteins due to heat and pressure treatment
    Iesel Van der Plancken
    Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Heverlee, Belgium
    J Agric Food Chem 53:5726-33. 2005
  6. ncbi Structural and functional studies of vertebrate metallothioneins: cross-talk between domains in the absence of physical contact
    Clemente Capasso
    CNR, Institute of Protein Biochemistry, via P. Castellino 111 I 80131 Naples, Italy
    Biochem J 391:95-103. 2005
  7. ncbi Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bonds
    Jane E Raulston
    Department of Microbiology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0579, USA
    Infect Immun 70:535-43. 2002
  8. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
  9. ncbi Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1
    J Y Hwang
    , , Germany
    FEBS Lett 508:355-9. 2001
  10. ncbi The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid)
    Zhao Feng
    Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
    Int J Biol Macromol 36:184-90. 2005

Research Grants

  1. REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONS
    SLOBODAN TODOROVIC; Fiscal Year: 2009
  2. ABCA1 agonist peptides for therapeutic use
    JOHN BIELICKI; Fiscal Year: 2007
  3. PEROXYNITRITE DAMAGE TO MICROTUBULE PROTEINS
    LISA LANDINO; Fiscal Year: 2007
  4. RESEARCH TRAINING IN DIABETES AND ENDOCRINOLOGY
    James May; Fiscal Year: 2007
  5. ASCORBIC ACID FUNCTION AND METABOLISM
    James May; Fiscal Year: 2007
  6. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2007
  7. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2007
  8. Vitamin C Transporters in the Brain
    James M May; Fiscal Year: 2010
  9. ASCORBIC ACID FUNCTION AND METABOLISM
    James M May; Fiscal Year: 2010
  10. REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONS
    SLOBODAN TODOROVIC; Fiscal Year: 2009

Detail Information

Publications124 found, 100 shown here

  1. ncbi Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction
    H J Ryser
    Department of Pathology, Boston University School of Medicine, MA 02118
    Proc Natl Acad Sci U S A 91:4559-63. 1994
    ..These findings suggest additional approaches to impede cell infection by HIV...
  2. ncbi The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell binding
    E Fenouillet
    Centre National de la Recherche Scientifique, Faculte de Medecine Nord, Marseille, France
    J Infect Dis 183:744-52. 2001
    ..The fact that the PDI catalytic activity present on lymphocytes is required for fusion supports the hypothesis that catalysts assist post-CD4 cell binding conformational changes within Env...
  3. ncbi Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entry
    Uthaman Gowthaman
    Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
    BMC Bioinformatics 9:S14. 2008
    ..This study attempts to perceive the mode of binding of dithionitrobenzoic acid (DTNB), and its structurally related compounds on PDI enzyme.
  4. ncbi Simple shifts in redox/thiol balance that perturb blood coagulation
    Henry K Bayele
    Department of Haematology, Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
    FEBS Lett 510:67-70. 2002
    ..These data also suggest that blood clotting may be influenced by unidentified redox or thiol equilibria...
  5. ncbi Changes in sulfhydryl content of egg white proteins due to heat and pressure treatment
    Iesel Van der Plancken
    Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Heverlee, Belgium
    J Agric Food Chem 53:5726-33. 2005
    ..Treatment conditions resulting in extensive protein insolubilization were accompanied by a transfer of free sulfhydryl groups from the soluble to the insoluble protein fraction. These SH groups were mainly accessible to DTNB...
  6. ncbi Structural and functional studies of vertebrate metallothioneins: cross-talk between domains in the absence of physical contact
    Clemente Capasso
    CNR, Institute of Protein Biochemistry, via P. Castellino 111 I 80131 Naples, Italy
    Biochem J 391:95-103. 2005
    ..Taken together, these results provide a strong argument in favour of the interaction of the two domains in the MT molecule, in spite of the elusive evidence provided by the structural analyses...
  7. ncbi Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bonds
    Jane E Raulston
    Department of Microbiology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614 0579, USA
    Infect Immun 70:535-43. 2002
    ..The accessibility of the Hsp70 substrate-binding domain may support the hypothesis that this protein plays a role in events following the initial stage of attachment instead of serving as a primary, surface-displayed adhesin...
  8. ncbi Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents
    S J McCormick
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Evansville, IN 47712, USA
    Acta Biochim Pol 48:573-8. 2001
    ..The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli...
  9. ncbi Calcium-dependent cysteine reactivities in the neuronal calcium sensor guanylate cyclase-activating protein 1
    J Y Hwang
    , , Germany
    FEBS Lett 508:355-9. 2001
    ..9 microM for Ca(2+) binding to this site with a fast off-rate (t approximately 2 ms). We conclude that the rapid dissociation of Ca(2+) from EF-hand 3 in GCAP-1 triggers activation of guanylate cyclase in rod cells...
  10. ncbi The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid)
    Zhao Feng
    Department of Biological Science and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
    Int J Biol Macromol 36:184-90. 2005
    ..The reactive cysteine of AK, which was proposed to be Cys274, may play an important role not in the binding of the transition-state analog but in the conformational changes caused by the transition-state analog...
  11. ncbi Cleavage of disulfide-linked fetuin-bisphosphonate conjugates with three physiological thiols
    Sufeng Zhang
    Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB T6G 2G6, Canada
    Biomacromolecules 6:2800-8. 2005
    ..In conclusion, disulfide-linked fetuin-thiolBP conjugates exhibited a high affinity to HA, which was readily lost upon cleavage with thiols found in physiological milieu...
  12. ncbi Modifications of cysteine residues in the solution and membrane-associated conformations of phosphatidylinositol transfer protein have differential effects on lipid transfer activity
    J M Tremblay
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
    Biochemistry 40:9151-8. 2001
    ..Furthermore, the inhibition of PITP activity by thiol-modifying reagents is a result of steric hindrance of phospholipid substrate binding...
  13. ncbi Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the hemoglobins of the domestic cat: acetylation of NH3+ terminal group of the beta chain transforms the complex pH dependence of the forward apparent second order rate constant to a simple form
    Kehinde O Okonjo
    Department of Chemistry, University of Ibadan, Ibadan, Nigeria
    Biophys Chem 119:196-204. 2006
    ..Analyses of the simple profiles gave pKas of 6.33 +/- 0.17 and 8.54 +/- 0.5 for HisH21[143]beta and other histidines within a distance of 2 nm of the sulfhydryl, and CysF9[93]beta of the minor hemoglobin, respectively...
  14. ncbi Critical flocculation concentrations, binding isotherms, and ligand exchange properties of peptide-modified gold nanoparticles studied by UV-visible, fluorescence, and time-correlated single photon counting spectroscopies
    Huan Xie
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Anal Chem 75:5797-805. 2003
    ..Full characterization of the composition and stability of peptide-modified metal nanoparticles is an important step in their use as intracellular delivery vectors and imaging agents...
  15. ncbi Identification of essential residues in 2',3'-cyclic nucleotide 3'-phosphodiesterase. Chemical modification and site-directed mutagenesis to investigate the role of cysteine and histidine residues in enzymatic activity
    J Lee
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 276:14804-13. 2001
    ..Taken together, these results demonstrate that both histidines assume critical roles for catalysis...
  16. ncbi Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase--a potential target for antimicrobial agents against Pseudomonas aeruginosa
    Roberto Velasco-García
    Laboratorio de Osmorregulación, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Avenida de Los Barrios, Tlalnepantla, Estado de Mexico, 54090 Mexico, Mexico
    Biochem Biophys Res Commun 341:408-15. 2006
    ..methyl-methanethiosulfonate inactivated the enzymes without affecting their stability while the bulkier dithionitrobenzoic acid (DTNB) and bis[diethylthiocarbamyl] disulfide (disulfiram) induced enzyme dissociation--at 23 degrees C--..
  17. ncbi Reversible reaction of 5,5'-dithiobis(2-nitrobenzoate) with the CysF9[93]beta sulfhydryl groups of the hemoglobins of the domestic cat: variation of the equilibrium and reverse rate constants with pH
    Kehinde Onwochei Okonjo
    Department of Chemistry, University of Ibadan, Ibadan, Nigeria
    Biophys Chem 121:65-73. 2006
    ..2+/-0.2 and 9.4+/-0.1 in the major hemoglobin and 6.7+/-0.3 and 8.4+/-0.1 in the minor hemoglobin...
  18. ncbi Different metabolizing ability of thiol reactants in human and rat blood: biochemical and pharmacological implications
    R Rossi
    Department of Neuroscience, Pharmacology Unit, Via A. Moro 4, University of Siena, Italy
    J Biol Chem 276:7004-10. 2001
    ..This is thought to influence significantly the biochemical, pharmacological, and toxicological effects of some drugs...
  19. ncbi On the nature of conformational openings: native and unfolded-state hydrogen and thiol-disulfide exchange studies of ferric aquomyoglobin
    Z Feng
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 314:153-66. 2001
    ..The C73 thiol group is exposed by a low-energy fluctuation that does not expose its amide group to exchange...
  20. ncbi Unfolding rates of barstar determined in native and low denaturant conditions indicate the presence of intermediates
    K Sridevi
    National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bangalore, India 560065
    Biochemistry 41:1568-78. 2002
    ....
  21. ncbi Identification and characterization of a critical region in the glycogen synthase from Escherichia coli
    Alejandra Yep
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    J Biol Chem 279:8359-67. 2004
    ..The results of this work indicate that this region is critical for catalysis and substrate binding...
  22. ncbi Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
    Arch Biochem Biophys 421:42-53. 2004
    ..The stoichiometry of histidine modification and the extent of inactivation showed that two essential histidine residues per active site are necessary for complete bovine liver OSC activity...
  23. ncbi Implications of the role of reactive cystein in arginine kinase: reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified arginine kinase reactivated by dithiothreitol
    Ji-Cheng Pan
    Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, PR China
    Biochem Biophys Res Commun 317:539-44. 2004
    ..The reactive cysteine of arginine kinase may play an important role not in the binding to the transition-state analog but in the conformational changes caused by the transition-state analog...
  24. ncbi Actin S-glutathionylation: evidence against a thiol-disulphide exchange mechanism
    Isabella Dalle-Donne
    Department of Biology, University of Milan, Milan, Italy
    Free Radic Biol Med 35:1185-93. 2003
    ....
  25. ncbi Roles for cysteine residues in the regulatory CXXC motif of human mitochondrial branched chain aminotransferase enzyme
    Myra E Conway
    Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, North Carolina 27157, USA
    Biochemistry 43:7356-64. 2004
    ..Thus, C315 appears to be the sensor for redox regulation of BCAT activity, whereas C318 acts as the "resolving cysteine", allowing for reversible formation of a disulfide bond...
  26. ncbi Antiviral activity of RhoA-derived peptides against respiratory syncytial virus is dependent on formation of peptide dimers
    Philip J Budge
    Department of Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Antimicrob Agents Chemother 47:3470-7. 2003
    ....
  27. ncbi Development and application of serum cholinesterase activity measurement using benzoylthiocholine iodide
    Susumu Osawa
    School of Medicine, Biological Sciences and Technology, Department of Health Sciences, Kyushu University, Higashi, Fukuoka City 812 8582, Japan
    Clin Chim Acta 351:65-72. 2005
    ..Many procedures using various artificial substrates have been developed but suffer from problems with substrate specificity and interference by endogenous substances...
  28. ncbi Single-molecule covalent chemistry with spatially separated reactants
    Tudor Luchian
    Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, TAMU 1114, College Station, TX 77843-1114, USA
    Angew Chem Int Ed Engl 42:3766-71. 2003
  29. ncbi Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin alpha2beta1
    Judith Lahav
    The Coagulation Laboratory, Rabin Medical Center Beilinson Campus, Zabotinski St, Petah Tiqva 49100, Israel
    Blood 102:2085-92. 2003
    ..These data suggest that the enzymatically catalyzed adhesion-associated reorganization of disulfide bonds is common to members of the integrin family and specific to this family...
  30. ncbi Kinetic studies of rat liver hexokinase D ('glucokinase') in non-co-operative conditions show an ordered mechanism with MgADP as the last product to be released
    Octavio Monasterio
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Biochem J 371:29-38. 2003
    ..In addition, the straight-line dependence observed when the reciprocal value of the inactivation constant was plotted against the sugar-ligand concentration supports the view that there is just one sugar-binding site in hexokinase D...
  31. ncbi Perturbation of microtubule polymerization by quercetin through tubulin binding: a novel mechanism of its antiproliferative activity
    Kamlesh Gupta
    BJM School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400 076, India
    Biochemistry 41:13029-38. 2002
    ..The data suggest that quercetin inhibits cancer cells proliferation at least in part by perturbing microtubule functions through tubulin binding...
  32. ncbi The roles of thiols in the bacterial organomercurial lyase (MerB)
    Keith E Pitts
    Department of Microbiology and Center for Metalloenzyme Studies, The University of Georgia, Athens, Georgia 30602, USA
    Biochemistry 41:10287-96. 2002
    ..On the basis of the foregoing we refine the previously proposed S(E)2 mechanism for protonolysis by MerB...
  33. ncbi Role of cysteine 306 in the catalytic mechanism of Ascaris suum phosphoenolpyruvate carboxykinase
    Sandra E Rios
    Department of Chemistry and Biochemistry, University of Notre Dame, IN 46556, USA
    Arch Biochem Biophys 404:25-37. 2002
    ..Alternatively, mutation of this residue could cause protein changes that interfere with the proper conformation of the nucleotides for optimal catalysis to take place...
  34. ncbi Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells
    C H Davis
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA
    Infect Immun 70:3413-8. 2002
    ..trachomatis serovar E entry...
  35. ncbi Thiol-disulphide interchange in tubulin: kinetics and the effect on polymerization
    P J Britto
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem J 389:549-58. 2005
    ..Under such conditions, the substituted tubulin forms protofilament-based structures such as microtubules, open tubules, sheets and/or bundles...
  36. ncbi Synthesis and characterization of a novel reagent containing dansyl group, which specifically alkylates sulfhydryl group: an example of application for protein chemistry
    Go Hasegawa
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
    J Biochem Biophys Methods 63:33-42. 2005
    ..These data showed that IADC is a useful reagent to specifically identify the positions of a Cys residue(s) in proteins...
  37. ncbi Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liver
    Hao Chen
    Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22, 14195 Berlin-Dahlem, Germany
    Glycobiology 12:65-71. 2002
    ....
  38. ncbi Reduced redox state allows prolonged survival of axotomized neonatal retinal ganglion cells
    L K Geiger
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, 600 Highland Avenue, Madison, WI 53792, USA
    Neuroscience 109:635-42. 2002
    ..Understanding the mechanisms by which ROS signal neuronal death could result in strategies for increasing their long-term survival after axonal injury...
  39. ncbi The role of cysteine residues in tellurite resistance mediated by the TehAB determinant
    M Dyllick-Brenzinger
    Structural Biology Research Group, University of Calgary, Calgary, Alberta, T2N 1N4, Canada
    Biochem Biophys Res Commun 277:394-400. 2000
    ..Studies using native and SDS denaturing PAGE under reducing and oxidizing conditions suggested that a cysteine in TehB is involved in binding tellurite...
  40. ncbi Studies on regulatory functions of malic enzymes. VII. Structural and functional characteristics of sulfhydryl groups in NADP-linked malic enzyme from Escherichia coli W
    M Iwakura
    J Biochem 86:1239-49. 1979
    ..The protective effect of NADP+ was increased by the addition of Mg2+. Based on the above results, the effects of Mg2+ on the SH-group modification are discussed from the viewpoint of conformational alteration of the enzyme...
  41. ncbi Characterization of chitosan thiolation and application to thiol quantification onto nanoparticle surface
    I Bravo Osuna
    Laboratoire de Physicochimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Universite Paris Sud 11, Faculte de Pharmacie, 5, rue J B Clement, 92296 Chatenay Malabry, France
    Int J Pharm 340:173-81. 2007
    ..It was successfully applied to the quantification of active thiol groups on the surface of poly(isobutyl cyanoacrylates) nanoparticles coated with thiol chitosan...
  42. ncbi Role of cysteine residues in 4-oxalomesaconate hydratase from Pseudomonas ochraceae NGJ1
    Suhong Li
    Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan
    Biosci Biotechnol Biochem 71:449-57. 2007
    ....
  43. ncbi Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase system
    Thomas J Jönsson
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Biochemistry 46:5709-21. 2007
    ..In addition, two new constructs have been generated, an AhpF-AhpC complex and a "twisted" form of AhpF, in which redox centers are locked together by stable disulfide bonds which mimic catalytic intermediates...
  44. ncbi Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes
    G Gao
    Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
    J Biol Chem 269:8234-9. 1994
    ..Residues 5-12 and 15 of this peptide are identical with a sequence of a yeast alkaline p-nitrophenylphosphatase, and the peptide has little homology with other mammalian phosphatases...
  45. ncbi Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinase
    Hua Wei He
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
    Int J Biol Macromol 41:361-8. 2007
    ..Furthermore, the findings in this study may provide a novel explanation for the low pKa value of Cys283...
  46. ncbi Nature and environment of the sulfhydryls of membrane-associated D-lactate dehydrogenase of Escherichia coli
    S R Dowd
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1252:278-83. 1995
    ..Cysteine 492 has been identified as containing the sulfhydryl that is accessible to the reagent under both conditions...
  47. ncbi Recombinant lysine:N(6)-hydroxylase: effect of cysteine-->alanine replacements on structural integrity and catalytic competence
    Scott Dick
    Department of Chemistry, University of Waterloo, ON, Canada
    Biochim Biophys Acta 1594:219-33. 2002
    ..A possible role of rIucD's Cys51 in the modulation of its mono-oxygenase function is discussed...
  48. ncbi Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coli
    M M Altamirano
    Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, D F
    Arch Biochem Biophys 258:95-100. 1987
    ..The results obtained show that sulfhydryls are not essential for catalysis or allosteric behavior of glucosamine-6-phosphate deaminase...
  49. ncbi A bicistronic expression system for bacterial production of authentic human interleukin-18
    Robert B Kirkpatrick
    Department of Gene Expression, Protein Biochemistry, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Rd, King of Prussia, PA 19406, USA
    Protein Expr Purif 27:279-92. 2003
    ....
  50. ncbi Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway
    Hang Mu
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China
    Int J Biol Macromol 41:439-46. 2007
    ....
  51. ncbi Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site
    Wen Jing Zhu
    College of Life Science, Shandong Agricultural University, Shandong Taian 271018, People s Republic of China
    Int J Biol Macromol 41:564-71. 2007
    ..Using the specific chemical modifier phenylglyoxal (PG) demonstrated that only one Arg, located near the ATP binding site, is essential for the activity of AK...
  52. ncbi Contribution of redox status to hepatitis C virus E2 envelope protein function and antigenicity
    Emmanuel Fenouillet
    UMR 6184, CNRS, Université Aix Marseille II, F 13015, France
    J Biol Chem 283:26340-8. 2008
    ..Although E2 function is surprisingly independent of its redox status, its disulfide bonds mask antigenic domains. E2 redox manipulation may contribute to improved vaccine design...
  53. ncbi Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coli
    M Yamaguchi
    J Biochem 86:325-33. 1979
    ..Based on the above results, the mechanism of the alteration of enzyme activities by sulfhydryl group modification is discussed...
  54. ncbi A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGK
    Matthew J Cliff
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
    J Mol Biol 364:810-23. 2006
    ..The success of the competition technique in characterizing this kinetic intermediate invites application to other model systems...
  55. ncbi Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis
    O Bjornberg
    Center for Enzyme Research, Institute of Molecular Biology, and Centre for Crystallograpic Studies, Department of Chemistry, University of Copenhagen, Copenhagen, Denmark
    Biochemistry 36:16197-205. 1997
    ..The possible acid-base chemistry of Cys 130 is compared to previous work on mammalian dihydropyrimidine dehydrogenases, flavoenzymes, which catalyze the reversed reaction, namely the reduction of pyrimidine bases...
  56. ncbi Mass spectrometric determination of the cleavage sites in Escherichia coli dihydroorotase induced by a cysteine-specific reagent
    R Daniel
    Laboratoire de Bioorganique et Biotechnologies associé au Centre National de la Recherche Scientifique, Ecole Nationale Superieure de Chimie de Paris, 11 rue P and M Curie, 75231 Paris Cedex 05, France
    J Biol Chem 272:26934-9. 1997
    ..Consistent with literature data, we assume that cysteine 221 and cysteine 265, along with the neighboring cysteines 263 and 268 arranged in cluster, are potential ligands of structural zinc of E. coli dihydroorotase...
  57. ncbi Zinc transfer potentials of the alpha - and beta-clusters of metallothionein are affected by domain interactions in the whole molecule
    L J Jiang
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Seeley G Mudd Building, 250 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:2503-8. 2000
    ..The two-domain structure of the whole molecule is important for its interaction with ligands and for control of its reactivity and overall conformation...
  58. ncbi Regulation of PTP1B via glutathionylation of the active site cysteine 215
    W C Barrett
    Laboratory of Biochemistry, Section of Metabolic Regulation, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892, USA
    Biochemistry 38:6699-705. 1999
    ..Glutathionylation of the cysteine sulfenic derivative will prevent the enzyme from further oxidation to its irreversible forms, and constitutes an efficient regulatory mechanism...
  59. ncbi Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid)
    M J Nozal
    Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain
    J Chromatogr A 778:347-53. 1997
    ..The detection limits are 0.21, 0.92 and 0.61 mumol/g wet sample for glutathione, cysteine and N-acetylcysteine, respectively...
  60. ncbi Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistry
    Arlin B Rogers
    Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Lab Invest 86:526-33. 2006
    ..These findings provide a molecular basis to improve the specificity of IHC and other immunoassays, and hold implications for antibody-based clinical diagnostics and therapeutics...
  61. ncbi Characterization of a mammalian peroxiredoxin that contains one conserved cysteine
    S W Kang
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:6303-11. 1998
    ..Thus, the primary cellular function of 1-Cys Prx appears to be to reduce peroxides with the use of electrons provided by an as yet unidentified source; the enzyme therefore represents a new type of peroxidase...
  62. ncbi DNA damage, apoptosis and cell cycle changes induced by fluoride in rat oral mucosal cells and hepatocytes
    Ling Fei He
    Department of Dental Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
    World J Gastroenterol 12:1144-8. 2006
    ..To study the effect of fluoride on oxidative stress, DNA damage and apoptosis as well as cell cycle of rat oral mucosal cells and hepatocytes...
  63. ncbi Comparative inhibition by hard and soft metal ions of steroid-binding capacity of renal mineralocorticoid receptor cross-linked to the 90-kDa heat-shock protein heterocomplex
    M D Galigniana
    Departamento de Qu approximately ímica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, and PHROM CONICET, 1428 Buenos Aires, Argentina
    Biochem J 341:585-92. 1999
    ..role played by these residues in steroid binding was confirmed by chemical modification of cysteines with dithionitrobenzoic acid, histidines with diethyl pyrocarbonate and acidic amino acids with Woodward's reagent (N-ethyl-5-..
  64. ncbi Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages
    Min Kyu Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    J Vet Med Sci 69:627-32. 2007
    ..GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay...
  65. ncbi Irreversible activation of cyclic nucleotide-gated ion channels by sulfhydryl-reactive derivatives of cyclic GMP
    R L Brown
    Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, Oregon 97209, USA
    Biochemistry 39:432-41. 2000
    ..Pretreatment of the patch with the sulfhydryl-blocking reagents N-ethylmaleimide (NEM) and bis-dithionitrobenzoic acid (DTNB) prevented covalent activation; the effect of DTNB was reversed by reduction with DTT...
  66. ncbi Selenium in thioredoxin reductase: a mechanistic perspective
    Brian M Lacey
    Department of Biochemistry, College of Medicine, University of Vermont, 89 Beaumont Avenue, Given Building Room B413, Burlington, Vermont 05405, USA
    Biochemistry 47:12810-21. 2008
    ..Further study of the biochemical properties of the truncated Cys and Sec TR enzymes demonstrates that the chemical advantage conferred on the eukaryotic enzyme by a selenol is the ability to function at acidic pH...
  67. ncbi Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid
    Wei Chen
    Department of Pharmaceutical Sciences, College of Pharmacy, South Dakota State University, Brookings, SD 57007, United States
    J Pharm Biomed Anal 48:1375-80. 2008
    ..This method should be applicable to cellular, subcellular, protein, or other biomatrix samples for thiol and disulfide quantification and will be a useful analytical method in the study of thiol redox state and thiol glutathionylation...
  68. ncbi Pyruvate dehydrogenase kinase isoform 2 activity stimulated by speeding up the rate of dissociation of ADP
    Haiying Bao
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    Biochemistry 43:13442-51. 2004
    ..We suggest that passing even limited reducing equivalents among lipoyl groups maintains E2 lipoyl domains in a conformation that aids kinase function...
  69. ncbi A visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase
    D Andrew Skaff
    Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Anal Biochem 396:96-102. 2010
    ..The high-throughput assay method has been successfully used to screen a library of more than 100,000 drug-like compounds and has identified both reversible and irreversible inhibitors of the human enzyme...
  70. ncbi Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum
    Chun You
    State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
    Biotechnol Bioeng 105:861-70. 2010
    ..at sites 50 and 201, rather than the disulfide bond formation which was ruled out by thiol titration with dithionitrobenzoic acid (DTNB)...
  71. ncbi Cholesteryl ester transfer protein promotes the formation of cholesterol-rich remnant like lipoprotein particles in human plasma
    Hiroshi Okamoto
    Central Pharmaceutical Research Institute, Japan Tobacco Inc, Takatsuki, Osaka, 569 1125, Japan
    Clin Chim Acta 375:92-8. 2007
    ..Cholesteryl ester transfer protein (CETP) is suggested to be involved in the cholesterol level in remnant like lipoprotein particles (RLP), but there is no direct evidence that CETP increases cholesterol-rich RLP in plasma...
  72. ncbi A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57
    Diego A Ellerman
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Davis, California 95616, USA
    Dev Cell 10:831-7. 2006
    ..The results support the idea that thiol-disulfide exchange is a mechanism that may act in gamete fusion to produce conformational changes in fusion-active proteins...
  73. ncbi Sublethal concentrations of diverse gold compounds inhibit mammalian cytosolic thioredoxin reductase (TrxR1)
    Yo Omata
    Hokkaido University, Sapporo, Japan
    Toxicol In Vitro 20:882-90. 2006
    ..Inhibition may be optimized to some degree by altering the ligand configuration of the compounds. These results support future study of a variety of Au compounds for therapeutic development as inhibitors of TrxR1...
  74. ncbi Simultaneous enzymatic kinetic determination of pesticides, carbaryl and phoxim, with the aid of chemometrics
    Yongnian Ni
    Department of Chemistry, Nanchang University, Nanchang 330047, China
    Anal Chim Acta 588:131-9. 2007
    ..2; phoxim=6.5), %Recovery (approx.105%) and %RPE(T) (6.5). Various spiked town-water samples produced recoveries in the range of 98.8-103% for each pesticide...
  75. ncbi Kinetic intermediates of unfolding of dimeric prostatic phosphatase
    Radosława Kuciel
    Chair of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Krakow, Poland
    Acta Biochim Pol 54:371-7. 2007
    ..Together with previous studies these results suggest that the stability of the catalytically active conformation of the enzyme depends mainly on the dimeric structure of the native hPAP...
  76. ncbi A colorimetric assay for S-adenosylhomocysteine hydrolase
    J D Lozada-Ramírez
    Department of Biochemistry and Molecular Biology A, Faculty of Biology, University of Murcia, Campus Espinardo, E 30071 Murcia, Spain
    J Biochem Biophys Methods 67:131-40. 2006
    ..This method is an easy, cheap and shorter alternative to more complex methods and it is applicable to routine clinical analysis and in the assay and development of new S-nucleosidylhomocysteines to be used as therapeutic compounds...
  77. ncbi Fatty acyl-CoA as an endogenous activator of UDP-glucuronosyltransferases
    Kazuharu Okamura
    Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan
    Biochem Biophys Res Commun 345:1649-56. 2006
    ..These results suggest that: (1) acyl-CoAs play a role as an endogenous activator of UGTs, and (2) a sulfhydryl group is required for the activation of UGT by physiological concentrations of acyl-CoAs...
  78. ncbi Metal binding ability of metallothionein-3 expressed in Escherichia coli
    Shigeru Toriumi
    Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Nishi-5, Sapporo 060-0810, Japan
    Basic Clin Pharmacol Toxicol 96:295-301. 2005
    ..However, the affinity of metallothionein-3 to Cu was much higher than that of metallothionein-1 and -2. The strong affinity to Cu of metallothionein-3 might be related to its growth inhibitory activity...
  79. ncbi Photodegradation mechanism and reaction kinetics of recombinant human interferon-alpha2a
    Hyong Ha Kim
    Health Metrology Group, Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, P O Box 102, Yuseong, Daejeon 305 600, Korea
    Photochem Photobiol Sci 6:171-80. 2007
    ..The relationship between the protein conformation and photobleaching rate could be explained based on the competition between excited state energy transfer and the photoionization process in Trp residues...
  80. ncbi Escherichia coli cyclopropane fatty acid synthase
    Fabienne Courtois
    Laboratoire de Chimie Organique Biologique, UMR7613 CNRS, Universite Pierre et Marie Curie, Paris, France
    Eur J Biochem 271:4769-78. 2004
    ..The inactivation is probably the result of steric hindrance, a phenomenon irrelevant to catalysis. It is very likely that E. coli CFAS operates via a carbocation mechanism, but the base and nucleophile remain to be identified...
  81. ncbi NAP1 catalyzes the formation of either positive or negative supercoils on DNA on basis of the dimer-tetramer equilibrium of histones H3/H4
    Sharon Peterson
    Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Biochemistry 46:8634-46. 2007
    ..It is proposed that these conformational changes in H3/H4 are involved in promoter activation and transcription elongation through nucleosomes...
  82. ncbi Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplus
    Jorge Moraes
    Laboratório de Química e Função de Proteínas e Peptídeos and Unidade de Experimentação Animal RJ, CBB, UENF, Avenida Alberto Lamego 2000, Horto, Campos dos Goytacazes, RJ, CEP 28015 620, Brazil
    Insect Biochem Mol Biol 41:400-9. 2011
    ..Furthermore, BmTIM was highly sensitive to the action of the thiol reagents dithionitrobenzoic acid and methyl methane thiosulfonate, suggesting that there are five cysteines exposed in each dimer and that ..
  83. ncbi The thiol group of the L-arabinose-binding protein. Chromophoric labeling and chemical identification of the sugar-binding site
    D M Miller
    J Biol Chem 254:7521-8. 1979
  84. ncbi Interaction of two sulfhydryl reagents with a cation recognition site on the neuronal dopamine carrier evidences small differences between [3H]GBR 12783 and [3H]cocaine binding sites
    F Refahi-Lyamani
    EP 076 du C N R S, U F R de Médecine and Pharmacie de Rouen, Saint Etienne du Rouvray, France
    Naunyn Schmiedebergs Arch Pharmacol 351:136-45. 1995
    ..When striatal cell membranes were treated in a medium containing 130 mmol/l Na+, there was a general decrease in the effects of ions on the reductions of specific binding produced by DTNB or pHMBS.(ABSTRACT TRUNCATED AT 400 WORDS)..
  85. ncbi Inhibition studies on calf pregastric esterase: the enzyme has no functional thiol group
    M Y Timmermans
    Department of Biochemistry, Limburgs Universitair Centrum, Diepenbeek, Belgium
    Biochem J 314:931-6. 1996
    ..The fact that PGE is not inhibited by thiol reagents indicates that PGE has no functional free thiol group. The PGE cDNA codes only for two cysteines, and their involvement in the formation of a disulphide bridge was demonstrated...
  86. ncbi Characterization of the denaturation and renaturation of human plasma vitronectin. II. Investigation into the mechanism of formation of multimers
    P Zhuang
    Department of Biochemistry, University of Tennessee, Knoxville, Tennessee 37996, USA
    J Biol Chem 271:14333-43. 1996
    ....
  87. ncbi Absolute on-line molecular mass analysis of basic fibroblast growth factor and its multimers by reversed-phase liquid chromatography with multi-angle laser light scattering detection
    I V Astafieva
    Wyatt Technology Corporation, Santa Barbara, CA 93103, USA
    J Chromatogr A 740:215-29. 1996
    ....
  88. ncbi Characteristics of rabbit muscle adenylate kinase inhibition by sulfur and recovery by dithiothreitol
    P J Russell
    Department of Biology, University of California, San Diego, La Jolla 92093, USA
    J Enzyme Inhib 9:179-94. 1995
    ..The results of these studies show that the activity of a small enzyme can be controlled in a manner analogous to the allosteric control of larger enzymes...
  89. ncbi Structural characterization of the metal binding site in the cysteine-rich region of HIV-1 Tat protein
    H W Huang
    Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China
    Biochem Biophys Res Commun 227:615-21. 1996
    ..Five out of seven Cys residues are coordinating with two Zn cations of the complex Zn-Tat21-38 (2:1). A model of the coordination arrangement of Zn binding sites at different states is proposed...
  90. ncbi The sulfhydryl groups of Cys 269 and Cys 272 are critical for the oligomeric state of chloroplast carbonic anhydrase from Pisum sativum
    H Bjorkbacka
    Department of Biochemistry, Umea University, Sweden
    Biochemistry 36:4287-94. 1997
    ..Furthermore, the data demonstrate that C269 and C272 are involved in the interaction between subunits and are necessary for a proper structure at the tetramer-tetramer interface...
  91. ncbi Contrasting effects of intracellular redox couples on the regulation of maxi-K channels in isolated myocytes from rabbit pulmonary artery
    D Thuringer
    CNRS UMR 6542, Faculte des Sciences et Techniques, Universite de Tours, France
    J Physiol 500:583-92. 1997
    ..The sulfhydryl-specific redox reagents (DTNB and BME) must act through another regulatory mechanism...
  92. ncbi Identification of the fifth axial heme ligand of chloroperoxidase
    S R Blanke
    Department of Biochemistry, University of Illinois, Urbana 61801
    J Biol Chem 263:18739-43. 1988
    ..Proteolysis and isolation of the labeled peptides using reverse phase HPLC and subsequent Edman sequence analysis detected and identified the thionitrobenzoate adducts of each of the three cysteinyl peptides of chloroperoxidase...
  93. ncbi Role of the essential thiol group in the thiol-activated cytolysin from Clostridium perfringens
    M Iwamoto
    Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan
    Eur J Biochem 167:425-30. 1987
    ....
  94. ncbi Reactivities of sulfhydryl groups in native and metal-free aminoacylase I
    D Heese
    Institut fur Physiologische Chemie, Universitat Marburg
    Biol Chem Hoppe Seyler 370:607-12. 1989
    ..It appears that binding of the inhibitor or removal of the metal ion induces conformational changes of the amino-acylase active site that render a buried sulfhydryl group more accessible to modification...
  95. ncbi Velocity of Ellman's reaction and its implication for kinetic studies in the millisecond time range
    J Stojan
    Institute of Biochemistry, Medical Faculty, Ljubljana, Slovenia
    Neurochem Res 17:1207-10. 1992
    ....
  96. ncbi Abnormal fibrinogens IJmuiden (B beta Arg14----Cys) and Nijmegen (B beta Arg44----Cys) form disulfide-linked fibrinogen-albumin complexes
    J Koopman
    IVVO TNO, Gaubius Laboratory, Leiden, The Netherlands
    Proc Natl Acad Sci U S A 89:3478-82. 1992
    ..We also found that a significant fraction of the abnormal fibrinogen molecules contained free sulfhydryl groups. These findings complicate interpretation of functional studies of these altered fibrinogens...
  97. ncbi Immunological assay of erythrocyte acetylcholinesterase
    J W Jones
    Blood Transfusion Department, Torbay Hospital, Plymouth, UK
    Clin Chim Acta 200:175-81. 1991
    ..The coefficient of variation for each method is 7%. Reference ranges have been established for healthy adults and cord blood...
  98. ncbi Thermostabilization of recombinant human and bovine CuZn superoxide dismutases by replacement of free cysteines
    R A Hallewell
    Chiron Corporation, Emeryville, CA 94608
    Biochem Biophys Res Commun 181:474-80. 1991
    ..In all cases, removal of a free cysteine residue increased thermostability...
  99. ncbi Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification
    S Kanaya
    Protein Engineering Research Institute, Osaka, Japan
    Biochem J 271:59-66. 1990
    ..Thus the sensitivity of E. coli RNAase H to N-ethylmaleimide arises not from blocking of the thiol group but from steric hindrance by the modifying group incorporated at either Cys-13 or Cys-133...
  100. ncbi Crystallization and structure solution at 4 A resolution of the recombinant synthase domain of N-(5'-phosphoribosyl)anthranilate isomerase:indole-3-glycerol-phosphate synthase from Escherichia coli complexed to a substrate analogue
    M Wilmanns
    Department of Structural Biology, University of Basel, Switzerland
    Protein Eng 3:173-80. 1990
    ..This system provides a model for an interface which is used in both intermolecular and intramolecular domain contacts...
  101. ncbi Organization of functional groups of liver bilitranslocase
    S Passamonti
    Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Universita degli Studi di Trieste, Italy
    Biochim Biophys Acta 1041:195-200. 1990
    ..On the contrary, the other class of sulfhydryl groups can be derivatized, regardless of the state of the arginine residues...

Research Grants55

  1. REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONS
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..
  2. ABCA1 agonist peptides for therapeutic use
    JOHN BIELICKI; Fiscal Year: 2007
    ..The research is likely to have a high impact on public health, since the therapeutics will be amenable to the widespread treatment of atherosclerosis. ..
  3. PEROXYNITRITE DAMAGE TO MICROTUBULE PROTEINS
    LISA LANDINO; Fiscal Year: 2007
    ..Researchers must consider and assess the effects of reactive molecules on abundant brain proteins because it is very likely that many proteins are damaged as we age. ..
  4. RESEARCH TRAINING IN DIABETES AND ENDOCRINOLOGY
    James May; Fiscal Year: 2007
    ..In the 31 years of NIH support, the program has supported 96 trainees, and over 70% have chosen careers in academia, industry, or government. ..
  5. ASCORBIC ACID FUNCTION AND METABOLISM
    James May; Fiscal Year: 2007
    ..By focusing on mechanisms in cell models and on early disease in animal models, this project will show whether and how vitamin C prevents atherosclerosis. ..
  6. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2007
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  7. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2007
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  8. Vitamin C Transporters in the Brain
    James M May; Fiscal Year: 2010
    ..Several neurodegenerative diseases including Alzheimer's and Parkinson's are associated with increased oxidant stress so that this project will help define the role of vitamin C in oxidant injury to the brain. ..
  9. ASCORBIC ACID FUNCTION AND METABOLISM
    James M May; Fiscal Year: 2010
    ..By focusing on mechanisms in cell models and on early disease in animal models, this project will show whether and how vitamin C prevents atherosclerosis. ..
  10. REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONS
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..
  11. Anesthetics block neuronal voltage-gated Ca2+ currents
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..abstract_text> ..
  12. ASCORBIC ACID FUNCTION AND METABOLISM
    James May; Fiscal Year: 1999
    ..We will also test the hypothesis that ascorbate-dependent recycling of alpha tocopherol in erythrocytes can replenish depleted stores of alpha tocopherol in human LDL. ..
  13. ANTIOXIDANT INTERACTIONS OF SELENIUM AND VITAMINS
    James May; Fiscal Year: 2002
    ....
  14. PRESERVATION OF HDL FUNCTION DURING EARLY ATHEROGENESIS
    JOHN BIELICKI; Fiscal Year: 2002
    ..Moreover, the research will uncover novel mechanisms for preserving HDL/LCAT function that can be utilized therapeutically to fight the onset of cardiovascular disease. ..
  15. ApoA-I Helical Domains and Cardiovascular Aging
    JOHN BIELICKI; Fiscal Year: 2005
    ..These studies are relevant for devising novel strategies to combat cardiovascular aging. ..
  16. Acetylcholinesterase: A Comparative Biochemical Approach
    Leo Pezzementi; Fiscal Year: 2005
    ..abstract_text> ..
  17. ASCORBIC ACID FUNCTION AND METABOLISM
    James May; Fiscal Year: 2004
    ....
  18. MULTIPLE CLASSES OF CA2+ CHANNELS - SENSORY TRANSMISSION
    SLOBODAN TODOROVIC; Fiscal Year: 2004
    ..abstract_text> ..
  19. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..