formaldehyde

Summary

Summary: A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)

Top Publications

  1. pmc FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin
    Paul G Giresi
    Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Genome Res 17:877-85. 2007
  2. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
  3. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
  4. doi MicroRNA profiling of clear cell renal cell carcinoma by whole-genome small RNA deep sequencing of paired frozen and formalin-fixed, paraffin-embedded tissue specimens
    Lihong Weng
    Department of Pathology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Pathol 222:41-51. 2010
  5. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
  6. pmc Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells
    Jinghuan Li
    Department of Histopathology, University of Dublin, Trinity College, Dublin, Ireland
    BMC Biotechnol 7:36. 2007
  7. pmc TRPA1 mediates formalin-induced pain
    Colleen R McNamara
    Hydra Biosciences, Inc, 790 Memorial Drive, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:13525-30. 2007
  8. doi Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues
    Maria Filippa Addis
    Porto Conte Ricerche Srl, Tramariglio, Alghero, Sassari, Italy
    Proteomics 9:3815-23. 2009
  9. pmc Is exposure to formaldehyde in air causally associated with leukemia?--A hypothesis-based weight-of-evidence analysis
    Lorenz R Rhomberg
    Gradient, Cambridge, Massachusetts 02138, USA
    Crit Rev Toxicol 41:555-621. 2011
  10. pmc Reproductive and developmental toxicity of formaldehyde: a systematic review
    Anh Duong
    School of Public Health, University of California, Berkeley, CA 94720, USA
    Mutat Res 728:118-38. 2011

Detail Information

Publications288 found, 100 shown here

  1. pmc FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) isolates active regulatory elements from human chromatin
    Paul G Giresi
    Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Genome Res 17:877-85. 2007
    ..demonstrate a simple procedure for the isolation of nucleosome-depleted DNA from human chromatin, termed FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements)...
  2. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
    ..This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine...
  3. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
    ..We have developed a gene expression assay (Whole-Genome DASL), capable of generating whole-genome gene expression profiles from degraded samples such as formalin-fixed, paraffin-embedded (FFPE) specimens...
  4. doi MicroRNA profiling of clear cell renal cell carcinoma by whole-genome small RNA deep sequencing of paired frozen and formalin-fixed, paraffin-embedded tissue specimens
    Lihong Weng
    Department of Pathology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Pathol 222:41-51. 2010
    ..To our knowledge, this is the first study to demonstrate that FFPE specimens can be used reliably for miRNA deep-sequencing analysis, making future large-scale clinical cohort/trial-based studies possible...
  5. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
    ..Lysine demethylation occurs via an oxidation reaction that generates formaldehyde. Importantly, RNAi inhibition of LSD1 causes an increase in H3 lysine 4 methylation and concomitant ..
  6. pmc Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells
    Jinghuan Li
    Department of Histopathology, University of Dublin, Trinity College, Dublin, Ireland
    BMC Biotechnol 7:36. 2007
    ..This study analyzed 160 miRNAs in paired snap frozen and FFPE cells to investigate if miRNAs may be successfully detected in archival specimens...
  7. pmc TRPA1 mediates formalin-induced pain
    Colleen R McNamara
    Hydra Biosciences, Inc, 790 Memorial Drive, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:13525-30. 2007
    ....
  8. doi Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues
    Maria Filippa Addis
    Porto Conte Ricerche Srl, Tramariglio, Alghero, Sassari, Italy
    Proteomics 9:3815-23. 2009
    ..An evaluation of the extent of modifications introduced on proteins by formalin fixation and crosslink reversal, and their impact on quality of MS results, is also reported...
  9. pmc Is exposure to formaldehyde in air causally associated with leukemia?--A hypothesis-based weight-of-evidence analysis
    Lorenz R Rhomberg
    Gradient, Cambridge, Massachusetts 02138, USA
    Crit Rev Toxicol 41:555-621. 2011
    Recent scientific debate has focused on the potential for inhaled formaldehyde to cause lymphohematopoietic cancers, particularly leukemias, in humans...
  10. pmc Reproductive and developmental toxicity of formaldehyde: a systematic review
    Anh Duong
    School of Public Health, University of California, Berkeley, CA 94720, USA
    Mutat Res 728:118-38. 2011
    b>Formaldehyde, the recently classified carcinogen and ubiquitous environmental contaminant, has long been suspected of causing adverse reproductive and developmental effects, but previous reviews were inconclusive, due in part, to ..
  11. pmc miRNA in situ hybridization in formaldehyde and EDC-fixed tissues
    John T G Pena
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10065, USA
    Nat Methods 6:139-41. 2009
    ..We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue sections, which can be prevented by fixation ..
  12. pmc Gene expression profiles from formalin fixed paraffin embedded breast cancer tissue are largely comparable to fresh frozen matched tissue
    Lorenza Mittempergher
    Divisions of Experimental Therapy, Molecular Biology, Surgical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e17163. 2011
    ..To this end we profiled, in duplicate, 20 FFPE tissues and 20 matched FF tissues and evaluated the concordance of the DASL results from FFPE and matched FF material...
  13. pmc Formaldehyde-inactivated whole-virus vaccine protects a murine model of enterovirus 71 encephalomyelitis against disease
    Kien Chai Ong
    Department of Pathology, University of Malaya, 50603 Kuala Lumpur, Malaysia
    J Virol 84:661-5. 2010
    ..b>Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were ..
  14. doi Urine formaldehyde level is inversely correlated to mini mental state examination scores in senile dementia
    Zhiqian Tong
    State Key Lab of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    Neurobiol Aging 32:31-41. 2011
    It is widely known that exogenous formaldehyde exposure induces human cognitive impairment and animal memory loss; and recent studies show that formaldehyde at pathological levels induces Aβ deposition and misfolding of tau protein to ..
  15. pmc Validation and reproducibility of a microarray-based gene expression test for tumor identification in formalin-fixed, paraffin-embedded specimens
    Raji Pillai
    Pathwork Diagnostics, Inc, Redwood City, California 94063 4737, USA
    J Mol Diagn 13:48-56. 2011
    ..3% concordance between laboratories. The Tissue of Origin Test makes the benefits of microarray-based gene expression tests for tumor diagnosis available for use with the most common type of histology specimen (ie, FFPE)...
  16. pmc Tumor tissue-derived formaldehyde and acidic microenvironment synergistically induce bone cancer pain
    Zhiqian Tong
    Neuroscience Research Institute, Peking University, Beijing, China
    PLoS ONE 5:e10234. 2010
    ..Accumulated evidence shows that endogenous formaldehyde concentrations are elevated in the blood or urine of patients with breast, prostate or bladder cancer...
  17. pmc Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples
    Yaguang Xi
    Cancer Genomics Laboratory, Mitchell Cancer Institute, Mobile, Alabama 36688, USA
    RNA 13:1668-74. 2007
    ..There are no significant differences of representative miRNA expression among 40 colorectal cancer FFPE specimens. This study provides a foundation for miRNA investigation using FFPE samples in cancer and other types of diseases...
  18. ncbi Quantitative protein analysis from formalin-fixed tissues: implications for translational clinical research and nanoscale molecular diagnosis
    K F Becker
    Technische Universitat, Institut fur Pathologie, Munchen, Germany
    J Pathol 211:370-8. 2007
    ..This technique may also be used to identify, characterize, and validate known and new protein markers in a variety of human diseases...
  19. pmc Formaldehyde-based whole-mount in situ hybridization method for planarians
    Bret J Pearson
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Dev Dyn 238:443-50. 2009
    ..Here, we describe a formaldehyde-based WISH method for the freshwater planarian Schmidtea mediterranea developed by systematically comparing and ..
  20. pmc Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and mutation-analysis
    Michal R Schweiger
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    PLoS ONE 4:e5548. 2009
    ..However, FFPE preparation is incompatible with many down-stream molecular biology techniques such as PCR based amplification methods and gene expression studies...
  21. ncbi Proteomic analysis of formalin-fixed prostate cancer tissue
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell Proteomics 4:1741-53. 2005
    ....
  22. ncbi Evidence for the involvement of spinal cord glia in subcutaneous formalin induced hyperalgesia in the rat
    L R Watkins
    Department of Psychology, University of Colorado at Boulder, 80309, USA
    Pain 71:225-35. 1997
    ..Taken together, the present series of experiments suggest an important role for spinal glial cells in the cascade of events that are initiated by descending signals following s.c. administration of formalin...
  23. ncbi Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue
    Stephen M Hewitt
    Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4605, USA
    Arch Pathol Lab Med 132:1929-35. 2008
    ..High-quality nucleic acids can be obtained from formalin-fixed, paraffin-embedded tissue, but greater attention to all steps in the process of tissue handling and preparation is required...
  24. pmc Mortality from lymphohematopoietic malignancies and brain cancer among embalmers exposed to formaldehyde
    Michael Hauptmann
    Department of Bioinformatics and Statistics, Netherlands Cancer Institute, Amsterdam, The Netherlands
    J Natl Cancer Inst 101:1696-708. 2009
    ..found in surveys of anatomists, pathologists, and funeral industry workers, all of whom may have worked with formaldehyde. We investigated the relation of mortality to work practices and formaldehyde exposure levels among these ..
  25. pmc Equivalence of protein inventories obtained from formalin-fixed paraffin-embedded and frozen tissue in multidimensional liquid chromatography-tandem mass spectrometry shotgun proteomic analysis
    Robert W Sprung
    Jim Ayers Institute for Precancer Detection and Diagnosis, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 8575, USA
    Mol Cell Proteomics 8:1988-98. 2009
    ..No covalent peptide modifications attributable to formaldehyde chemistry were detected by analyses of the MS/MS datasets, which suggests that undetected, cross-linked ..
  26. doi Gene expression profiling of formalin-fixed, paraffin-embedded familial breast tumours using the whole genome-DASL assay
    Nic Waddell
    The Queensland Institute of Medical Research, Brisbane, Australia
    J Pathol 221:452-61. 2010
    ....
  27. pmc Quantitative expression profiling in formalin-fixed paraffin-embedded samples by affymetrix microarrays
    Diana Abdueva
    Department of Pathology, Children s Hospital Los Angeles, Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    J Mol Diagn 12:409-17. 2010
    ..This study provides guidelines for application of these methods in the discovery, validation, and clinical application of microarray expression profiling with FFPE material...
  28. pmc Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay
    Mark Abramovitz
    VM Institute of Research, Montreal, Quebec, Canada
    Biotechniques 44:417-23. 2008
    ....
  29. ncbi Direct cancer tissue proteomics: a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues
    S I Hwang
    Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT 06030, USA
    Oncogene 26:65-76. 2007
    ..We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types...
  30. doi Effect of inhaled formaldehyde on learning and memory of mice
    Z Lu
    College of Life Science, Central China Normal University, Wuhan, China
    Indoor Air 18:77-83. 2008
    In this study, we investigated the effect of inhaled formaldehyde on learning and memory capacity...
  31. pmc Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 25:664-75. 2012
    b>Formaldehyde is a known human and animal carcinogen that forms DNA adducts, and causes mutations. While there is widespread exposure to formaldehyde in the environment, formaldehyde is also an essential biochemical in all living cells...
  32. pmc Random DNA fragmentation allows detection of single-copy, single-exon alterations of copy number by oligonucleotide array CGH in clinical FFPE samples
    Galen Hostetter
    Translational Genomics Research Institute, 445 N 5th Street, Phoenix, AZ 85004, USA
    Nucleic Acids Res 38:e9. 2010
    ..3. Finally we present data on derivative of log ratio, a particular sensitive detector of measurement variance, for 216 sequential hybridizations to assess protocol reliability over a wide range of FFPE samples...
  33. pmc Isolation of active regulatory elements from eukaryotic chromatin using FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)
    Paul G Giresi
    Department of Biology and Carolina Center for the Genome Sciences, University of North Carolina at Chapel Hill, CB 3280, 408 Fordham Hall, Chapel Hill, NC 27599 3280, USA
    Methods 48:233-9. 2009
    ..a simple procedure for the genome-wide isolation of nucleosome-depleted DNA from human chromatin, termed FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)...
  34. doi Proteome, phosphoproteome, and N-glycoproteome are quantitatively preserved in formalin-fixed paraffin-embedded tissue and analyzable by high-resolution mass spectrometry
    Paweł Ostasiewicz
    Department for Proteomics and Signal Transduction at Max Planck Institute for Biochemistry, 82152 Martinsried, Germany
    J Proteome Res 9:3688-700. 2010
    ..Thus, FFPE biobank material can be analyzed by quantitative proteomics at the level of proteins and post-translational modifications...
  35. pmc Primary central nervous system lymphomas: a validation study of array-based comparative genomic hybridization in formalin-fixed paraffin-embedded tumor specimens
    Esteban Braggio
    Department of Biochemistry, Mayo Clinic, Scottsdale, Arizona, USA
    Clin Cancer Res 17:4245-53. 2011
    ....
  36. pmc Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry
    Rita Casadonte
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA
    Nat Protoc 6:1695-709. 2011
    ..For example, TMA analysis of 300 FFPE cores would typically require 6 h of total time through data acquisition, not including data analysis...
  37. pmc Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity
    Martin Kerick
    Max Planck Institute for Molecular Genetics, Ihnestr, Berlin, Germany
    BMC Med Genomics 4:68. 2011
    ..However, these technologies need to be further improved with regard to reproducibility and applicability to clinical samples and settings...
  38. doi Producing reverse phase protein microarrays from formalin-fixed tissues
    Claudia Wolff
    Institut fur Pathologie, Technische Universitat Munchen, Munich, Germany
    Methods Mol Biol 785:123-40. 2011
    ..In this chapter, we describe a protein extraction protocol established in our laboratory combined with the use of reverse phase protein microarray...
  39. doi Combined analysis of chromosomal aberrations and glutathione S-transferase M1 and T1 polymorphisms in pathologists occupationally exposed to formaldehyde
    Alfredo Santovito
    Department of Animal and Human Biology, University of Torino, Via Accademia Albertina n 13, 10123, Turin, Italy
    Arch Toxicol 85:1295-302. 2011
    The formaldehyde (FA) genotoxic potential in occupationally exposed individuals is conflicting...
  40. pmc Optimization of formaldehyde cross-linking for protein interaction analysis of non-tagged integrin beta1
    Cordula Klockenbusch
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T1Z3, Canada
    J Biomed Biotechnol 2010:927585. 2010
    b>Formaldehyde cross-linking of protein complexes combined with immunoprecipitation and mass spectrometry analysis is a promising technique for analysing protein-protein interactions, including those of transient nature...
  41. doi Genotoxicity biomarkers in occupational exposure to formaldehyde--the case of histopathology laboratories
    Carina Ladeira
    Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa, Portugal
    Mutat Res 721:15-20. 2011
    b>Formaldehyde, classified by the IARC as carcinogenic in humans and experimental animals, is a chemical agent that is widely used in histopathology laboratories...
  42. doi Formaldehyde and tobacco smoke as alkylating agents: the formation of N-methylenvaline in pathologists and in plastic laminate workers
    Roberto Bono
    Department of Public Health and Microbiology, University of Torino, Torino, Italy
    Sci Total Environ 414:701-7. 2012
    Aim of this study was to investigate the relationships between the concentration of formaldehyde in air and the alkylation of hemoglobin to form a terminal N-methylenvaline residue in three occupationally exposed groups: a) technicians of ..
  43. pmc Mortality from lymphohematopoietic malignancies among workers in formaldehyde industries: the National Cancer Institute Cohort
    Laura E Beane Freeman
    Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Natl Cancer Inst 101:751-61. 2009
    b>Formaldehyde exposure is associated with leukemia in some epidemiological studies...
  44. pmc Multicentre validation study of nucleic acids extraction from FFPE tissues
    Serena Bonin
    ACADEM Department University of Trieste and ICGEB, University of Trieste, Cattinara Hospital, 34149 Trieste, Italy
    Virchows Arch 457:309-17. 2010
    ..The results of the study emphasize the need for quality control of the nucleic acid extracts with standardised methods to prevent false negative results and to allow data comparison among different diagnostic laboratories...
  45. doi Incomplete follow-up in the National Cancer Institute's formaldehyde worker study and the impact on subsequent reanalyses and causal evaluations
    Gary M Marsh
    Center for Occupational Biostatistics and Epidemiology and Department of Biostatistics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Regul Toxicol Pharmacol 58:233-6. 2010
    ..performed reanalyses of the National Cancer Institute cohort study on nasopharyngeal cancer (NPC) risk among formaldehyde exposed workers (Hauptmann et al., 2004). Both reanalyses (Marsh and Youk, 2005; Marsh et al...
  46. doi Histology without formalin?
    René J Buesa
    Ann Diagn Pathol 12:387-96. 2008
    ..With a fast penetration, formaldehyde is a slow and reversible fixative that requires 24 to 48 hours to completely bind to tissue; thus, any surgical ..
  47. ncbi The generation of formaldehyde in cigarettes--Overview and recent experiments
    Richard R Baker
    Group Research and Development, British American Tobacco, Regent s Park Road, Southampton SO15 8TL, UK
    Food Chem Toxicol 44:1799-822. 2006
    ..One exception to this generalisation is formaldehyde, which is generated from saccharides used as tobacco ingredients...
  48. ncbi Treatment of maternal blood samples with formaldehyde does not alter the proportion of circulatory fetal nucleic acids (DNA and mRNA) in maternal plasma
    Satheesh Kumar Reddy Chinnapapagari
    Department of Research, University Women s Hospital, Basel, Switzerland
    Clin Chem 51:652-5. 2005
  49. ncbi Whole genome SNP arrays using DNA derived from formalin-fixed, paraffin-embedded ovarian tumor tissue
    Ella R Thompson
    VBCRC Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    Hum Mutat 26:384-9. 2005
    ..This advance will open up vast archival tissue resources to high-resolution genetic analysis and unlock a wealth of biological information...
  50. ncbi Formaldehyde: integrating dosimetry, cytotoxicity, and genomics to understand dose-dependent transitions for an endogenous compound
    Melvin E Andersen
    Program in Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 118:716-31. 2010
    b>Formaldehyde (FA), an endogenous cellular aldehyde, is a rat nasal carcinogen...
  51. pmc Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis
    Hakima Achkor
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Plant Physiol 132:2248-55. 2003
    The ADH2 gene codes for the Arabidopsis glutathione-dependent formaldehyde dehydrogenase (FALDH), an enzyme involved in formaldehyde metabolism in eukaryotes...
  52. doi Clinical proteomic applications of formalin-fixed paraffin-embedded tissues
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, National Cancer Institute at Frederick, 1050 Boyles Street, Frederick, MD 21702, USA
    Clin Lab Med 29:101-13. 2009
    ..The almost inexhaustible supply of these tissues will enable proteomic laboratories access to clinically important specimens that will undoubtedly lead to a number of important discoveries in the near future...
  53. pmc The function neutralizing anti-TrkA antibody MNAC13 reduces inflammatory and neuropathic pain
    Gabriele Ugolini
    Lay Line Genomics, Via di Castel Romano 100, 00128 Rome, Italy
    Proc Natl Acad Sci U S A 104:2985-90. 2007
    ..This study represents a direct demonstration that neutralizing antibodies directed against the TrkA receptor may display potent analgesic effects in inflammatory and chronic pain...
  54. ncbi Lack of dramatic enrichment of fetal DNA in maternal plasma by formaldehyde treatment
    Grace T Y Chung
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, China
    Clin Chem 51:655-8. 2005
  55. pmc Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM
    Lasse S Kristensen
    Institute of Human Genetics, University of Aarhus, The Bartholin Building, Aarhus C, Denmark
    BMC Cancer 9:453. 2009
    ..Sensitive Melting Analysis after Real Time - Methylation Specific PCR (SMART-MSP) and Methylation Sensitive - High Resolution Melting (MS-HRM) are two methods for single locus DNA methylation detection based on HRM...
  56. pmc Genome-wide comparison of paired fresh frozen and formalin-fixed paraffin-embedded gliomas by custom BAC and oligonucleotide array comparative genomic hybridization: facilitating analysis of archival gliomas
    Gayatry Mohapatra
    Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Acta Neuropathol 121:529-43. 2011
    ..In combination, these advances should facilitate genome-wide analysis of rare, small and/or histologically heterogeneous gliomas from FFPE tissues...
  57. doi Quantitation of p95HER2 in paraffin sections by using a p95-specific antibody and correlation with outcome in a cohort of trastuzumab-treated breast cancer patients
    Jeff Sperinde
    diaDexus, South San Francisco, CA, USA
    Clin Cancer Res 16:4226-35. 2010
    ..We sought to show that quantitative p95HER2 levels would correlate with outcome in trastuzumab-treated HER2-positive metastatic breast cancer...
  58. pmc Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues
    Martina Doleshal
    Asuragen, Inc, Austin, TX 78744, USA
    J Mol Diagn 10:203-11. 2008
    ....
  59. ncbi Epidemiological studies of formaldehyde exposure and risk of leukemia and nasopharyngeal cancer: a meta-analysis
    Annette M Bachand
    Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado, USA
    Crit Rev Toxicol 40:85-100. 2010
    The authors conducted meta-analyses of the epidemiological literature on formaldehyde exposure and risk of leukemia and risk of nasopharyngeal cancer...
  60. pmc A temporal threshold for formaldehyde crosslinking and fixation
    Lars Schmiedeberg
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
    PLoS ONE 4:e4636. 2009
    b>Formaldehyde crosslinking is in widespread use as a biological fixative for microscopy and molecular biology...
  61. doi Protein unlocking procedures of formalin-fixed paraffin-embedded tissues: application to MALDI-TOF imaging MS investigations
    Maurizio Ronci
    Centro Studi Sull Invecchiamento, Chieti, Italy
    Proteomics 8:3702-14. 2008
    ..A multivariate statistical evaluation is presented and discussed according to molecular spatial distributions and tissue morphology...
  62. ncbi Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR)
    Thi Thu Huyen Nguyen
    Institute for Microbiology, Ernst Moritz Arndt University of Greifswald, D 17487 Greifswald, Germany
    Mol Microbiol 71:876-94. 2009
    ..We analysed the global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA)...
  63. pmc Treatment of inflammatory and neuropathic pain by uncoupling Src from the NMDA receptor complex
    Xue Jun Liu
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Nat Med 14:1325-32. 2008
    ....
  64. ncbi Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells
    Günter Speit
    Universitat Ulm, Abteilung Humangenetik, D 89070 Ulm, Germany
    Mutat Res 613:1-9. 2006
    b>Formaldehyde (FA) is genotoxic in vitro in cultured mammalian cells. When FA reaches the nuclear DNA, it forms DNA-protein cross-links (DPX)...
  65. pmc KRAS genotyping of paraffin-embedded colorectal cancer tissue in routine diagnostics: comparison of methods and impact of histology
    Wilko Weichert
    Institut fur Pathologie, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    J Mol Diagn 12:35-42. 2010
    ....
  66. pmc Occupational exposure to formaldehyde, hematotoxicity, and leukemia-specific chromosome changes in cultured myeloid progenitor cells
    Luoping Zhang
    School of Public Health, University of California at Berkeley, Berkeley, California 94720, USA
    Cancer Epidemiol Biomarkers Prev 19:80-8. 2010
    There are concerns about the health effects of formaldehyde exposure, including carcinogenicity, in light of elevated indoor air levels in new homes and occupational exposures experienced by workers in health care, embalming, ..
  67. pmc Formaldehyde exposure and asthma in children: a systematic review
    Gerald McGwin
    Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Environ Health Perspect 118:313-7. 2010
    Despite multiple published studies regarding the association between formaldehyde exposure and childhood asthma, a consistent association has not been identified...
  68. pmc Formaldehyde and leukemia: epidemiology, potential mechanisms, and implications for risk assessment
    Luoping Zhang
    School of Public Health, University of California, Berkeley, California 94720 7356, USA
    Environ Mol Mutagen 51:181-91. 2010
    b>Formaldehyde is widely used in the United States and other countries. Occupational and environmental exposures to formaldehyde may be associated with an increased risk of leukemia in exposed individuals...
  69. pmc Cancer effects of formaldehyde: a proposal for an indoor air guideline value
    Gunnar Damgård Nielsen
    National Research Centre for the Working Environment, Lersø Parkallé 105, 2100, Copenhagen Ø, Denmark
    Arch Toxicol 84:423-46. 2010
    b>Formaldehyde is a ubiquitous indoor air pollutant that is classified as "Carcinogenic to humans (Group 1)" (IARC, Formaldehyde, 2-butoxyethanol and 1-tert-butoxypropanol-2-ol...
  70. pmc Analyses and interpretation of whole-genome gene expression from formalin-fixed paraffin-embedded tissue: an illustration with breast cancer tissues
    Muhammad G Kibriya
    Department of Health Studies, The University of Chicago, 5841 S, Maryland Avenue, MC 2007, Chicago, IL 60637, USA
    BMC Genomics 11:622. 2010
    ..We used a whole genome DASL assay (addressing 18,391 genes) on a total of 72 samples from paired breast tumor and surrounding healthy tissues from both FF and FFPE samples...
  71. ncbi 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues
    Carol B Fowler
    Department of Biophysics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 87:836-46. 2007
    ..been hampered by inefficient methods to extract proteins from archival tissue and by an incomplete knowledge of formaldehyde-induced modifications in proteins...
  72. pmc Exon-array profiling unlocks clinically and biologically relevant gene signatures from formalin-fixed paraffin-embedded tumour samples
    J S Hall
    Translational Radiobiology Group, School of Cancer and enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Wilmslow Road, Manchester M20 4BX, UK
    Br J Cancer 104:971-81. 2011
    ..This study aimed to show that useful information can be obtained by Exon-array profiling archival FFPE tumour samples...
  73. doi Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon 1.0 microarrays
    Kim Linton
    Cancer Research UK Department of Medical Oncology, the Christie NHS Foundation Trust, Manchester, UK
    Biotechniques 47:587-96. 2009
    ..While larger series are needed to confirm high correspondence between fresh-frozen and FFPE expression patterns, these data suggest that both Plus 2.0 and Exon arrays are suitable platforms for FFPE microarray expression analyses...
  74. doi Metastatic colorectal cancer KRAS genotyping in routine practice: results and pitfalls
    Aude Lamy
    Laboratory of Tumor Genetics, University Hospital, Rouen, France
    Mod Pathol 24:1090-100. 2011
    ..The tumor heterogeneity observed in some patients indicates that it should be more appropriate to perform KRAS genotyping on metastases if sample is available...
  75. ncbi Formalin-fixed paraffin-embedded (FFPE) proteome analysis using gel-free and gel-based proteomics
    Omid Azimzadeh
    Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Institute of Radiation Biology, 85764 Neuherberg, Germany
    J Proteome Res 9:4710-20. 2010
    ..This study will facilitate the development of future proteomic analysis of FFPE tissue and provide a tool for the validation in archival samples of biomarkers of exposure, prognosis and disease...
  76. ncbi Mass spectrometric analysis of formalin-fixed paraffin-embedded tissue: unlocking the proteome within
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Proteomics 6:4106-14. 2006
    ..Although these developments are quite new, the ability to conduct MS-based proteomic analyses of FFPE tissues opens heretofore intractable clinical samples for discovery-based biomarker research...
  77. ncbi In vivo stabilization of preinitiation complexes by formaldehyde cross-linking
    LEOS VALASEK
    Institute of Microbiology, AS CR, Prague, Czech Republic
    Methods Enzymol 429:163-83. 2007
    ..Therefore, we developed an alternative method for PIC stabilization using formaldehyde (HCHO) to cross-link factors associated with 40S ribosomal subunits in vivo before the disruption of the yeast ..
  78. ncbi Efficient method for the proteomic analysis of fixed and embedded tissues
    Darryl Erik Palmer-Toy
    Harvard Partners Center for Genetics and Genomics, Cambridge, Massachusetts, USA
    J Proteome Res 4:2404-11. 2005
    ..Moreover, we demonstrate that a high level of sequence coverage can be observed for proteins of interest...
  79. pmc Protein extraction of formalin-fixed, paraffin-embedded tissue enables robust proteomic profiles by mass spectrometry
    Marshall S Scicchitano
    Department of Safety Assessment, 709 Swedeland Road, Mail Stop UE0364, King of Prussia, PA 19406, USA
    J Histochem Cytochem 57:849-60. 2009
    ..These findings demonstrate that formalin fixation, paraffin processing, and LCM do not negatively impact protein quality and quantity as determined by MS and that FFPE samples are amenable to global proteomic analysis...
  80. pmc Evaluation of archival time on shotgun proteomics of formalin-fixed and paraffin-embedded tissues
    Brian M Balgley
    Calibrant Biosystems, Gaithersburg, Maryland 20878, USA
    J Proteome Res 8:917-25. 2009
    ....
  81. doi Formaldehyde exposure and leukemia: a new meta-analysis and potential mechanisms
    Luoping Zhang
    School of Public Health, 50 University Hall, University of California, Berkeley, CA 94720 7356, USA
    Mutat Res 681:150-68. 2009
    b>Formaldehyde is an economically important chemical, to which more than 2 million U.S. workers are occupationally exposed...
  82. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Histochem Cytochem 50:1005-11. 2002
    ..These findings may lead to a more effective protocol for DNA extraction from archival paraffin-embedded tissues and may also provide enhanced understanding of changes that occur during formalin-induced modification of nucleic acids...
  83. pmc Mortality among a cohort of garment workers exposed to formaldehyde: an update
    L E Pinkerton
    Industrywide Studies Branch, Division of Surveillance, Hazard Evaluations and Field Studies, The National Institute for Occupational Safety and Health, Cincinnati, Ohio 45226, USA
    Occup Environ Med 61:193-200. 2004
    To evaluate the mortality experience of 11 039 workers exposed to formaldehyde for three months or more in three garment plants. The mean time weighted average formaldehyde exposure at the plants in the early 1980s was 0...
  84. ncbi Social modulation of pain as evidence for empathy in mice
    Dale J Langford
    Department of Psychology and Centre for Research on Pain, McGill University, Montreal, QC H3A 1B1, Canada
    Science 312:1967-70. 2006
    ..Finally, observation of a cagemate in pain altered pain sensitivity of an entirely different modality, suggesting that nociceptive mechanisms in general are sensitized...
  85. doi Genome-wide DNA methylation analysis of archival formalin-fixed paraffin-embedded tissue using the Illumina Infinium HumanMethylation27 BeadChip
    Christina Thirlwell
    Medical Genomics Group, UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
    Methods 52:248-54. 2010
    ..Here, we describe a protocol for the analysis of FFPE samples using the Infinium HumanMethylation27 BeadChip...
  86. ncbi AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli
    Pål Ø Falnes
    Centre for Molecular Biology and Neuroscience, and Institute of Medical Microbiology, University of Oslo, National Hospital, 0027 Oslo, Norway
    Nature 419:178-82. 2002
    ..This mechanism represents a new pathway for DNA repair and the third type of DNA damage reversal mechanism so far discovered...
  87. pmc Formate as the main branch point for methylotrophic metabolism in Methylobacterium extorquens AM1
    Gregory J Crowther
    Department of Chemical Engineering, University of Washington, Box 355014, Seattle, Washington 98195 5014, USA
    J Bacteriol 190:5057-62. 2008
    ..In these bacteria, two routes are possible for generating methylene H4F from formaldehyde during methylotrophic growth: one involving the reaction of formaldehyde with H4F to generate methylene H4F and ..
  88. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine HMR 204, 2011 Zonal Avenue, Los Angeles 90033, USA
    Histochem Cell Biol 122:211-8. 2004
    ..1 M alkaline solution is a simpler and more effective heat-induced retrieval protocol for DNA extraction. Combination with some chemicals (detergents) may further significantly improve efficiency of the heat-induced retrieval technique...
  89. ncbi Proteome analysis of microdissected formalin-fixed and paraffin-embedded tissue specimens
    Tong Guo
    Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA
    J Histochem Cytochem 55:763-72. 2007
    ..This large degree of protein overlapping is attributed to the application of detergent-based protein extraction in both the cell pellet preparation protocol and the AR technique...
  90. ncbi Protein extraction from formalin-fixed, paraffin-embedded tissue sections: quality evaluation by mass spectrometry
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine HMR 204, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    J Histochem Cytochem 54:739-43. 2006
    ....
  91. doi Antigen retrieval for proteomic characterization of formalin-fixed and paraffin-embedded tissues
    Haifeng Xu
    Calibrant Biosystems, 910 Clopper Road, Suite 220N, Gaithersburg, Maryland 20878, USA
    J Proteome Res 7:1098-108. 2008
    ..Because single peptide hits are often regarded with skepticism, it is notable that all proteins tested by IHC stained positive...
  92. pmc Successful protein extraction from over-fixed and long-term stored formalin-fixed tissues
    Claudia Wolff
    Institute of Pathology, Technische Universitat Munchen, Munich, Germany
    PLoS ONE 6:e16353. 2011
    ..Because tissue fixation times vary in the routine clinical setting and pathology archives contain billions of FFPE tissues blocks, our data are highly relevant for research, diagnosis, and treatment of disease...
  93. ncbi Reliability and reproducibility of PCR-based testing of O6-methylguanine-DNA methyltransferase gene (MGMT) promoter methylation status in formalin-fixed and paraffin-embedded neurosurgical biopsy specimens
    M Preusser
    Department of Internal Medicine I, Medical University, Austria
    Clin Neuropathol 27:388-90. 2008
    ..To analyze the reliability and reproducibility of PCR-based testing of O6-methylguanine-DNA methyltransferase gene (MGMT) promoter methylation status in formalin-fixed and paraffin-embedded (FFPE) neurosurgical biopsy specimens...
  94. pmc Development of a microarray platform for FFPET profiling: application to the classification of human tumors
    Sven Duenwald
    Translational Sciences, Department of Molecular Profiling, Merck Research Laboratories, Seattle, WA 98109, USA
    J Transl Med 7:65. 2009
    ..Methods are needed to profile these FFPET samples that are linked to clinical outcomes to generate hypotheses that could lead to classifiers for clinical applications...
  95. pmc Whole genome amplification for array comparative genomic hybridization using DNA extracted from formalin-fixed, paraffin-embedded histological sections
    Jian Huang
    International Agency for Research on Cancer, Lyon, France
    J Mol Diagn 11:109-16. 2009
    ..0001). These results suggest that genetic analyses are possible using WGA-DNA extracted from paraffin sections, but that they should be performed with a carefully optimized and controlled protocol...
  96. pmc An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples
    Xiao Zhang
    Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
    J Mol Diagn 10:513-9. 2008
    ..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...
  97. ncbi Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges
    Niroshini J Nirmalan
    Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, St James s University Hospital, Beckett Street, Leeds, UK
    Mol Biosyst 4:712-20. 2008
    ..Here, we critically review the current status of research in the field, highlighting challenges which need to be addressed for robust quantitative application of protocols to ensure confident high impact inferences can be made...
  98. ncbi Housing characteristics and indoor concentrations of nitrogen dioxide and formaldehyde in Quebec City, Canada
    Nicolas L Gilbert
    Air Health Effects Division, Health Canada, 269 Laurier Avenue West, PL 4903B, Ottawa, Ontario, Canada K1A 0K9
    Environ Res 102:1-8. 2006
    Concentrations of nitrogen dioxide and formaldehyde were determined in a study of 96 homes in Quebec City, Canada, between January and April 2005...
  99. doi Reversal of pathological pain through specific spinal GABAA receptor subtypes
    Julia Knabl
    Institute of Experimental and Clinical Pharmacology and Toxicology, University of Erlangen Nurnberg, D 91054 Erlangen, Germany
    Nature 451:330-4. 2008
    ..These results provide a rational basis for the development of subtype-selective GABAergic drugs for the treatment of chronic pain, which is often refractory to classical analgesics...
  100. ncbi Formaldehyde and cancer risk: a quantitative review of cohort studies through 2006
    C Bosetti
    Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy
    Ann Oncol 19:29-43. 2008
    Occupational exposure to formaldehyde has been associated with excess risk of nasopharyngeal and selected other cancers.
  101. ncbi Effect of duration of fixation on quantitative reverse transcription polymerase chain reaction analyses
    Maricris Macabeo-Ong
    Oral Pathology, Department of Stomatology, University of California San Francisco, California 94143 0424, USA
    Mod Pathol 15:979-87. 2002
    ..However, results for some markers are adversely affected by prolonged formalin fixation times...

Research Grants80

  1. Bioengineering Core for Cellular and Tissue Models
    Andrew McCulloch; Fiscal Year: 2009
    ..that are not used for gene expression analysis are stretched to some known % stretch and fixed in 2% PFA (para-formaldehyde) diluted in 50% PBS (phosphate-buffered saline) and 50% modified Hepes buffered cardioplegic solution...
  2. Nanopore Biosensor for Kinetics of Reparative Antibodies on Plasma Membranes
    Sang Hyun Oh; Fiscal Year: 2010
    ..Fixation of any kind (methanol, formaldehyde, freezing) destroys the complex membrane antigen...
  3. Sleep in Rats: From Conceptualization to Measurement, Analysis and Interpretation
    Gayle Giboney Page; Fiscal Year: 2010
    ..The relevance of this proposed award to human health is to discern the contributions and consequences of disrupted sleep as it relates to the development of persistent pain. ..
  4. PLASMA PROTEINS: MARKERS OF CHEMICAL EXPOSURE
    GHULAM ANSARI; Fiscal Year: 1990
    ..Industrial chemicals containing functional groups such as aldehyde (acrolein, formaldehyde, and glutaraldehyde), epoxide (ethylene oxide, propylene oxide, and styrene oxide), and hydrocarbon (benzene, ..
  5. SYNTHESIS & APPLICATIONS OF LABELLED FORMYLATING REAGENTS: AMINO ACIDS & PEPTIDE
    MANOUCHEHR SALJOUGHIAN; Fiscal Year: 1999
    ..Several formylating reagents such as formaldehyde, formic acid, formyl fluoride, formic anhydride or formic acetic anhydride have been available for non-..
  6. Innate immune evasion and/or antagonism by eastern equine encephalitis virus
    KATHERINE RYMAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  7. Innate immune evasion and/or antagonism by eastern equine encephalitis virus
    KATHERINE RYMAN; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  8. True Direct Single Molecule RNA Sequencing
    PATRICE MARIE MILOS; Fiscal Year: 2010
    ....
  9. FLORIDA FIREFIGHTER CANCER INCIDENCE AND MORTALITY
    Lora Fleming; Fiscal Year: 2001
    ..routinely exposed to a variety of carcinogenic substances, such as benzene, polycyclic aromatic hydrocarbons, formaldehyde, and asbestos...
  10. FLORIDA FIREFIGHTER CANCER INCIDENCE AND MORTALITY
    Lora Fleming; Fiscal Year: 2000
    ..routinely exposed to a variety of carcinogenic substances, such as benzene, polycyclic aromatic hydrocarbons, formaldehyde, and asbestos...
  11. Validation and Quantification of FFPE Antigen Retrieval by Proteome Analysis
    Satya Saxena; Fiscal Year: 2009
    ....
  12. Validation and Quantification of FFPE Antigen Retrieval by Proteome Analysis
    XUPEING FANG; Fiscal Year: 2010
    ....
  13. A Mucosal RSV Vaccine
    Kejian Yang; Fiscal Year: 2012
    ..The reduced incidence of disease in the lower respiratory tract would reduce the amount of medical costs directly associated with the infection. ..
  14. NITROSAMINE METABOLISM AND ESOPHAGEAL CANCER
    SIDNEY MIRVISH; Fiscal Year: 1992
    ..human tissues to establish the tissue specificity, survey the microsomal metabolism in 20 human esophagi, study formaldehyde (CH20) formation from [3Hmethyl]NMAA, and test non-P450 cofactors and effects of monoclonal antibodies that ..
  15. Developing an inactivated whole cell H. pylori vaccine
    GARY NABORS; Fiscal Year: 2005
    ..This trial will position the vaccine for more highly powered Phase 1 or early Phase 2 trials, and thus will move H. pylori vaccination one step closer to being a reality. ..
  16. Developing an inactivated whole cell H. pylori vaccine
    GARY NABORS; Fiscal Year: 2004
    ..This trial will position the vaccine for more highly powered Phase 1 or early Phase 2 trials, and thus will move H. pylori vaccination one step closer to being a reality. ..
  17. Developing an inactivated whole cell H. pylori vaccine
    GARY NABORS; Fiscal Year: 2006
    ..This trial will position the vaccine for more highly powered Phase 1 or early Phase 2 trials, and thus will move H. pylori vaccination one step closer to being a reality. ..
  18. DNA Vaccines for Q Fever
    David Pascual; Fiscal Year: 2009
    ..This research project fits within the RMRCE Integrated Research Focus on Immunomodulation, Adjuvants, and Vaccines, and will interact with RP 1.1 and RP 1.2 and utilize the resources of Coxiella Core. ..
  19. MOLECULAR EPIDEMIOLOGY OF CT DETECTED LUNG CANCER
    Joel Weissfeld; Fiscal Year: 2001
    ..abstract_text> ..
  20. Immunoprotective monoclonals to B anthracis spores
    ALISON O BRIEN; Fiscal Year: 2002
    ..actually bind to the surface of spores and decrease the level of spore germination, taken with the fact that formaldehyde-inactivated spores can serve as a protective vaccine against anthrax in guinea pigs, led us to the following ..
  21. Immunoprotective monoclonals to B. anthracis spores
    ALISON O BRIEN; Fiscal Year: 2003
    ..actually bind to the surface of spores and decrease the level of spore germination, taken with the fact that formaldehyde-inactivated spores can serve as a protective vaccine against anthrax in guinea pigs, led us to the following ..
  22. THE EFFECT OF HCG ON SERTOLI CELLS
    CAROLYN CONNELL; Fiscal Year: 1980
    ..The testes will be fixed in situ by perfusion with formaldehyde-glutaraldehyde-picric acid (FGP)...
  23. Molecular signatures of melanoma histology and progression: A Population Based A
    Abrar A Qureshi; Fiscal Year: 2010
    ..Thus we aim to significantly improve the current paradigm for diagnosis and therapy for melanoma by eventually reducing mortality from this deadly skin cancer. ..
  24. Molecular signatures of melanoma histology and progression: A Population Based A
    Abrar Qureshi; Fiscal Year: 2009
    ..Thus we aim to significantly improve the current paradigm for diagnosis and therapy for melanoma by eventually reducing mortality from this deadly skin cancer. ..
  25. HEAD AND NECK CARCINOMA CELL KINETICS
    Marc Coltrera; Fiscal Year: 1990
    ..As none of the currently available proliferation-specific monoclonal antibodies can be used in formaldehyde-fixed, paraffin-embedded tissues, new proliferation-specific monoclonal antibodies capable of reacting with S-..
  26. Formaldehyde and Other Pollutants from Ozone Reactions on Ventilation Systems
    Hugo Destaillats; Fiscal Year: 2009
    ..purpose of this project is to improve understanding of the formation of harmful gas- phase pollutants, such as formaldehyde, and aerosol particles in the indoor work environment...
  27. Recovery of RNA from Formalin-Fixed Tissues
    TIMOTHY O LEARY; Fiscal Year: 2006
    ..The objective of this proposal is to identify the formaldehyde-induced chemical modifications that occur to nucleic acids during histologic tissue processing and to develop ..
  28. Early Diagnosis of Oral Carcinoma Using Mutant PP32R1
    GERALD KOCHEVAR; Fiscal Year: 2005
    ..Identification of the intracellular protein ligands will provide a specific functional context for mutant PP32R1S. ..
  29. Early Diagnosis of Oral Carcinoma Using Mutant PP32R1
    GERALD KOCHEVAR; Fiscal Year: 2006
    ..Identification of the intracellular protein ligands will provide a specific functional context for mutant PP32R1S. ..
  30. HDAC Inhibitors and CTCL
    Louise Showe; Fiscal Year: 2009
    ..Understanding the characteristics that define why one patient responds and another patient does not, can lead to the identification of ways to convert the non-responsive patients to responsive ones. ..
  31. Improving Antibodies to Virion Based HIV Vaccines
    Kathie Grovit Ferbas; Fiscal Year: 2009
    ..To this end, we have used Env modified formaldehyde and heat treated virions and pseudovirions as tools to study correlates of Ab mediated protection...
  32. RSV Upper Airway Infection and Otitis Media
    Joan Durbin; Fiscal Year: 2009
    ..Our group has found a new vaccine against this virus, and want to test whether immunity to RSV can protect against the process that causes bacterial ear infections. ..
  33. RSV Upper Airway Infection and Otitis Media
    JOAN E contact DURBIN; Fiscal Year: 2010
    ..Our group has found a new vaccine against this virus, and want to test whether immunity to RSV can protect against the process that causes bacterial ear infections. ..
  34. Towards identification of prognostic gene sets in breast cancer: The E2197 Trial
    Brian Leyland Jones; Fiscal Year: 2010
    ....
  35. TUMOR MARKERS TO PREDICT TREATMENT RESPONSE
    Lynn Dressler; Fiscal Year: 2002
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
  36. TUMOR MARKERS TO PREDICT TREATMENT RESPONSE
    Lynn Dressler; Fiscal Year: 2003
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
  37. PAIN, OPIOID ANALGESIA AND BLOOD PRESSURE CONTROL
    Bradley Taylor; Fiscal Year: 2000
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
  38. PAIN, OPIOID ANALGESIA AND BLOOD PRESSURE CONTROL
    Bradley Taylor; Fiscal Year: 2001
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
  39. PAIN, OPIOID ANALGESIA AND BLOOD PRESSURE CONTROL
    Bradley Taylor; Fiscal Year: 1999
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
  40. TUMOR MARKERS TO PREDICT TREATMENT RESPONSE
    Lynn Dressler; Fiscal Year: 2001
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
  41. PAIN, OPIOID ANALGESIA AND BLOOD PRESSURE CONTROL
    Bradley Taylor; Fiscal Year: 2002
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
  42. PAIN, OPIOID ANALGESIA AND BLOOD PRESSURE CONTROL
    Bradley Taylor; Fiscal Year: 1999
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
  43. TUMOR MARKERS TO PREDICT TREATMENT RESPONSE
    Lynn Dressler; Fiscal Year: 2004
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
  44. PAIN, OPIOID ANALGESIA AND BLOOD PRESSURE CONTROL
    Bradley Taylor; Fiscal Year: 2000
    ..The results of these studies will facilitate our understanding of the mechanisms that contribute to chronic pain in the hypertensive patient. ..
  45. TUMOR MARKERS TO PREDICT TREATMENT RESPONSE
    Lynn Dressler; Fiscal Year: 2005
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
  46. CORE--NEUROPATHOLOGY AND GENETICS
    Ronald Hamilton; Fiscal Year: 2005
    ..abstract_text> ..
  47. A high-resolution in situ proteomics atlas of salivary gland development
    Melinda Larsen; Fiscal Year: 2010
    ..These studies will provide insight into the fundamental signaling events occurring during the process of salivary gland development and differentiation. ..
  48. A high-resolution in situ proteomics atlas of salivary gland development
    Melinda Larsen; Fiscal Year: 2009
    ..These studies will provide insight into the fundamental signaling events occurring during the process of salivary gland development and differentiation. ..
  49. Formaldehyde and Other Pollutants from Ozone Reactions on Ventilation Systems
    Hugo Destaillats; Fiscal Year: 2010
    ..purpose of this project is to improve understanding of the formation of harmful gas- phase pollutants, such as formaldehyde, and aerosol particles in the indoor work environment...
  50. DNA-Methylation Profiling from Fixed Melanocytic Tissues
    NANCY E contact THOMAS; Fiscal Year: 2010
    ..This study will be a first step toward the development of diagnostic methylation assays that could be used to standardize melanoma diagnosis, thereby decreasing under- and over-treatment of melanocytic lesions. ..
  51. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2005
    ..A distinguishing feature of methylotrophic metabolism is the generation and consumption of formaldehyde as a central intermediate, the starting point for all of metabolism...
  52. miRNA HD Array Platform
    Bruce E Seligmann; Fiscal Year: 2010
    ..In Phase II, this platform will be applied to identify host cell miRNA signatures useful for screening for infection by Category A, B and C pathogens, and useful as targets for drug discovery. ..
  53. NEW DRUGS FOR RESISTANT DISEASE FROM CI/MS STUDIES
    VERONICA BIERBAUM; Fiscal Year: 1999
    ..Preliminary studies indicate that formaldehyde plays a critical role in this mechanism, and that newly synthesized anthracycline-formaldehyde conjugates ..
  54. Placental Treg Activation and Pregnancy Outcome in the FIV-infected Cat Model
    GREGORY TODD PHARR; Fiscal Year: 2010
    ..The purpose of this project is to evaluate the effect of HIV infection on the function of regulatory T cells, using the feline immunodeficiency virus (FIV)-infected cat to model maternal-fetal infection and compromised pregnancy. ..
  55. Placental Treg Activation and Pregnancy Outcome in the FIV-infected Cat Model
    KAREN COATS; Fiscal Year: 2009
    ..The purpose of this project is to evaluate the effect of HIV infection on the function of regulatory T cells, using the feline immunodeficiency virus (FIV)-infected cat to model maternal-fetal infection and compromised pregnancy. ..
  56. NEW DRUGS FOR RESISTANT DISEASE FROM CI/MS STUDIES
    VERONICA BIERBAUM; Fiscal Year: 2000
    ..Preliminary studies indicate that formaldehyde plays a critical role in this mechanism, and that newly synthesized anthracycline-formaldehyde conjugates ..
  57. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2004
    ..A distinguishing feature of methylotrophic metabolism is the generation and consumption of formaldehyde as a central intermediate, the starting point for all of metabolism...
  58. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2007
    ..A distinguishing feature of methylotrophic metabolism is the generation and consumption of formaldehyde as a central intermediate, the starting point for all of metabolism...
  59. DNA-Methylation Profiling from Fixed Melanocytic Tissues
    Nancy Thomas; Fiscal Year: 2009
    ..This study will be a first step toward the development of diagnostic methylation assays that could be used to standardize melanoma diagnosis, thereby decreasing under- and over-treatment of melanocytic lesions. ..
  60. miRNA HD Array Platform
    Bruce Seligmann; Fiscal Year: 2009
    ..In Phase II, this platform will be applied to identify host cell miRNA signatures useful for screening for infection by Category A, B and C pathogens, and useful as targets for drug discovery. ..
  61. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2006
    ..A distinguishing feature of methylotrophic metabolism is the generation and consumption of formaldehyde as a central intermediate, the starting point for all of metabolism...
  62. Develop & Validate An Ultrasound Tissue Preservation De*
    Zhenqing Zhu; Fiscal Year: 2006
    ..Long-term stability of tissue morphology and mRNA integrity in USFFPE tissues is slightly better than that in conventional FFPE tissues. [unreadable] [unreadable]..
  63. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2003
    ..From a cell biology perspective, their ability to funnel all of their cell carbon through a toxic intermediate (formaldehyde) make them of interest in terms of how they balance formaldehyde production and consumption under a wide regime ..
  64. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 1999
    ..From a cell biology perspective, their ability to funnel all of their cell carbon through a toxic intermediate (formaldehyde) make them of interest in terms of how they balance formaldehyde production and consumption under a wide regime ..
  65. LOW-DOSE RADIATION STERILIZATION
    SHALABY SHALABY; Fiscal Year: 2000
    ..of a novel radiochemical sterilization (RC-S) process comprising a combination of a controllably released formaldehyde, and low-dose (equal to or less than 7...
  66. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2001
    ..From a cell biology perspective, their ability to funnel all of their cell carbon through a toxic intermediate (formaldehyde) make them of interest in terms of how they balance formaldehyde production and consumption under a wide regime ..
  67. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2000
    ..From a cell biology perspective, their ability to funnel all of their cell carbon through a toxic intermediate (formaldehyde) make them of interest in terms of how they balance formaldehyde production and consumption under a wide regime ..
  68. REGULATION OF C1 METABOLISM IN METHYLOTROPHS
    Mary Lidstrom; Fiscal Year: 2002
    ..From a cell biology perspective, their ability to funnel all of their cell carbon through a toxic intermediate (formaldehyde) make them of interest in terms of how they balance formaldehyde production and consumption under a wide regime ..
  69. In vivo RNAi nanoparticle cancer gene function reagent
    Martin Woodle; Fiscal Year: 2009
    ..If successful, the resulting reagent is expect to enable rapid advances in understanding this life threatening disease that produce better targeted therapeutic treatment options for patients. ..
  70. Chromatin Immunoprecipitation (ChIP) Assay Development for FFPE human brain secti
    Mary Anne Jelinek; Fiscal Year: 2010
    ..Phase I proposal , we intend to develop a Clinical Chromatin ImmunoPrecipitation assay (C- ChIP), for use with formaldehyde-fixed paraffin embedded (FFPE) human brain specimens, which will enable analysis of changes in histone post-..
  71. NASOPHARYNGEAL CARCINOMA --DUST AND SMOKE IN MALAYSIA
    R Armstrong; Fiscal Year: 1990
    ..The specific aims are to: 1) test the hypothesis that occupational exposure to specific dusts, smokes, formaldehyde, and aromatic hydrocarbons in Selangor Malaysia are risk factors for NPC; 2) to estimate risk of NPC ..
  72. Translation of Predictive Cancer Biomarkers into Clinical Practice
    TIMOTHY J contact TRICHE; Fiscal Year: 2010
    ....
  73. Translation of Predictive Cancer Biomarkers into Clinical Practice
    Timothy Triche; Fiscal Year: 2009
    ....