microfilament proteins

Summary

Summary: Filaments which are composed primarily of actin and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.

Top Publications

  1. pmc Organizing the cell cortex: the role of ERM proteins
    Richard G Fehon
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Nat Rev Mol Cell Biol 11:276-87. 2010
  2. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
  3. ncbi Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
    M Maekawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8315, Japan
    Science 285:895-8. 1999
  4. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
  5. ncbi The revised Ghent nosology for the Marfan syndrome
    Bart L Loeys
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Gent, Belgium
    J Med Genet 47:476-85. 2010
  6. ncbi Proteins of the ADF/cofilin family: essential regulators of actin dynamics
    J R Bamburg
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 80523, USA
    Annu Rev Cell Dev Biol 15:185-230. 1999
  7. pmc Genome-wide association study identifies novel breast cancer susceptibility loci
    Douglas F Easton
    CR UK Genetic Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge CB1 8RN, UK
    Nature 447:1087-93. 2007
  8. pmc Losartan, an AT1 antagonist, prevents aortic aneurysm in a mouse model of Marfan syndrome
    Jennifer P Habashi
    Howard Hughes Medical Institute and Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 312:117-21. 2006
  9. ncbi A modular design for the clathrin- and actin-mediated endocytosis machinery
    Marko Kaksonen
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Cell 123:305-20. 2005
  10. pmc miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer
    T Chiyomaru
    Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8 35 1 Sakuragaoka, Kagoshima, Japan
    Br J Cancer 102:883-91. 2010

Detail Information

Publications314 found, 100 shown here

  1. pmc Organizing the cell cortex: the role of ERM proteins
    Richard G Fehon
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    Nat Rev Mol Cell Biol 11:276-87. 2010
    ..Recent studies examining the structure and in vivo functions of ERMs have greatly advanced our understanding of the importance of membrane-cytoskeleton interactions...
  2. ncbi Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  3. ncbi Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
    M Maekawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8315, Japan
    Science 285:895-8. 1999
    ..These results indicate that phosphorylation of LIM-kinase by ROCK and consequently increased phosphorylation of cofilin by LIM-kinase contribute to Rho-induced reorganization of the actin cytoskeleton...
  4. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
    ..2005), support a model in which Sun proteins tether nesprins in the ONM via interactions spanning the PNS. In this way, Sun proteins and nesprins form a complex that links the nucleoskeleton and cytoskeleton (the LINC complex)...
  5. ncbi The revised Ghent nosology for the Marfan syndrome
    Bart L Loeys
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Gent, Belgium
    J Med Genet 47:476-85. 2010
    ..We anticipate that these new guidelines may delay a definitive diagnosis of MFS but will decrease the risk of premature or misdiagnosis and facilitate worldwide discussion of risk and follow-up/management guidelines...
  6. ncbi Proteins of the ADF/cofilin family: essential regulators of actin dynamics
    J R Bamburg
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 80523, USA
    Annu Rev Cell Dev Biol 15:185-230. 1999
    ..ADF/cofilins are essential for cytokinesis, phagocytosis, fluid phase endocytosis, and other cellular processes dependent upon actin dynamics...
  7. pmc Genome-wide association study identifies novel breast cancer susceptibility loci
    Douglas F Easton
    CR UK Genetic Epidemiology Unit, Department of Public Health and Primary Care, University of Cambridge, Cambridge CB1 8RN, UK
    Nature 447:1087-93. 2007
    ..05 level compared with an estimated 1,343 that would be expected by chance, indicating that many additional common susceptibility alleles may be identifiable by this approach...
  8. pmc Losartan, an AT1 antagonist, prevents aortic aneurysm in a mouse model of Marfan syndrome
    Jennifer P Habashi
    Howard Hughes Medical Institute and Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 312:117-21. 2006
    ....
  9. ncbi A modular design for the clathrin- and actin-mediated endocytosis machinery
    Marko Kaksonen
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Cell 123:305-20. 2005
    ....
  10. pmc miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer
    T Chiyomaru
    Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8 35 1 Sakuragaoka, Kagoshima, Japan
    Br J Cancer 102:883-91. 2010
    ..We have recently identified down-regulated microRNAs including miR-145 and miR-133a in bladder cancer (BC). The aim of this study is to determine the genes targeted by miR-145, which is the most down-regulated microRNA in BC...
  11. pmc Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin
    Hideki Yamaguchi
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 168:441-52. 2005
    ..These results indicate that EGF receptor signaling regulates invadopodium formation through the N-WASP-Arp2/3 pathway and cofilin is necessary for the stabilization and maturation of invadopodia...
  12. pmc Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules
    Monther Al-Alwan
    Stem Cell Therapy Program, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia
    PLoS ONE 6:e27339. 2011
    ....
  13. pmc Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
    T Matsui
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 140:647-57. 1998
    ....
  14. ncbi A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage
    Y Imai
    Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan
    Biochem Biophys Res Commun 224:855-62. 1996
    ..Iba1 protein is therefore suggested to act as an adapter molecule, mediating calcium signals that may function in a monocytic lineage including microglia...
  15. pmc Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness
    P J Stewart-Hutchinson
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Exp Cell Res 314:1892-905. 2008
    ....
  16. ncbi miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma
    Masayuki Kano
    Department of Frontier Surgery, Chiba University Graduate School of Medicine, Chiba, Japan
    Int J Cancer 127:2804-14. 2010
    ..The identification of tumor-suppressive miRNAs, miR-145, miR-133a and miR-133b, directly control oncogenic FSCN1 gene. These signal pathways of ESCC could provide new insights into potential mechanisms of ESCC carcinogenesis...
  17. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
    ..Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin...
  18. pmc Ezrin, Radixin and Moesin: key regulators of membrane-cortex interactions and signaling
    Amanda L Neisch
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Curr Opin Cell Biol 23:377-82. 2011
    ..In this review we discuss recent evidence in a variety of cells indicating that ERMs serve as scaffolds to facilitate efficient signal transduction on the cytoplasmic face of the plasma membrane...
  19. pmc Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization
    Kyle J Roux
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 106:2194-9. 2009
    ..These effects can be accounted for entirely by Nesp4's kinesin-binding function. The implication is that Nesp4 may contribute to microtubule-dependent nuclear positioning...
  20. pmc Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
    R Gary
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Cell 6:1061-75. 1995
    ..The conformationally inaccessible C-terminal region included the F-actin binding site, suggesting that this activity is likewise regulated by masking...
  21. ncbi Unleashing formins to remodel the actin and microtubule cytoskeletons
    Melissa A Chesarone
    Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Rev Mol Cell Biol 11:62-74. 2010
    ..The emerging complexity in the mechanisms governing formins mirrors the wide range of essential functions that they perform in cell motility, cell division and cell and tissue morphogenesis...
  22. ncbi Nesprin-1 and -2 are involved in the pathogenesis of Emery Dreifuss muscular dystrophy and are critical for nuclear envelope integrity
    Qiuping Zhang
    Department of Medicine, University of Cambridge, Cambridge, UK
    Hum Mol Genet 16:2816-33. 2007
    ..Taken together, these data suggest that EDMD may be caused, in part, by uncoupling of the nucleoskeleton and cytoskeleton because of perturbed nesprin/emerin/lamin interactions...
  23. ncbi Periodic lamellipodial contractions correlate with rearward actin waves
    Gregory Giannone
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Cell 116:431-43. 2004
    ..We propose that an actin bound, contraction-activated signaling complex is transported locally from the tip to the base of the lamellipodium, activating the next contraction/extension cycle...
  24. pmc Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility
    M F Carlier
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    J Cell Biol 136:1307-22. 1997
    ..In conclusion, the function of ADF is not to sequester G-actin. ADF uses ATP hydrolysis in actin assembly to enhance filament dynamics...
  25. pmc The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion
    Ang Li
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK
    Curr Biol 20:339-45. 2010
    ..We provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells, and we compare invadopodia with invasive filopod-like structures in 3D...
  26. pmc Afadin: A key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis
    W Ikeda
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka 565 0871, Japan
    J Cell Biol 146:1117-32. 1999
    ....
  27. pmc Molecular requirements for actin-based lamella formation in Drosophila S2 cells
    Stephen L Rogers
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107, USA
    J Cell Biol 162:1079-88. 2003
    ..Our results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells...
  28. pmc Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins
    Raffi Tonikian
    Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
    PLoS Biol 7:e1000218. 2009
    ....
  29. pmc Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks
    Timothy S Gomez
    Department of Immunology, Division of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA
    Mol Biol Cell 23:3215-28. 2012
    ....
  30. ncbi Elastic behavior of cross-linked and bundled actin networks
    M L Gardel
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Science 304:1301-5. 2004
    ..We parameterize the full range of behavior in a state diagram and elucidate its origin with a robust model...
  31. pmc WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function
    Joshua T Piotrowski
    Department of Immunology, College of Medicine, Mayo Clinic, Rochester, Minnesota, USA
    Mol Cell Biol 33:958-73. 2013
    ..Aberrant trafficking of these key T cell proteins may potentially lead to attenuated proliferation and effector function...
  32. pmc Multiple novel nesprin-1 and nesprin-2 variants act as versatile tissue-specific intracellular scaffolds
    Dipen Rajgor
    Cardiovascular Division, James Black Centre, King s College London, London, United Kingdom
    PLoS ONE 7:e40098. 2012
    ..However emerging data suggests that the nesprins have multiple alternative start and termination sites throughout their genes allowing the generation of smaller isoforms...
  33. ncbi Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer
    Danijela Vignjevic
    UMR 144 Centre National de la Recherche Scientifique and Department of Pathology, Institut Curie, 25 rue d Ulm, 75248 Paris Cedex 05, France
    Cancer Res 67:6844-53. 2007
    ..We propose that genes involved in cell migration and invasion, such as fascin1, could serve as novel targets for metastasis prevention...
  34. ncbi Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle
    Qiuping Zhang
    Department of Medicine, ACCI, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
    J Cell Sci 118:673-87. 2005
    ..Moreover, its association at the NE with lamin and emerin, the genes mutated in Emery-Dreifuss muscular dystrophy, suggests a mechanism to explain how disruption of the NE leads to muscle dysfunction...
  35. ncbi The immunoglobulin-like cell adhesion molecule nectin and its associated protein afadin
    Yoshimi Takai
    Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Annu Rev Cell Dev Biol 24:309-42. 2008
    ..Additionally, nectins regulate many other cellular activities (such as movement, proliferation, survival, differentiation, polarization, and the entry of viruses) in cooperation with other CAMs and cell surface membrane receptors...
  36. ncbi The roles of fascins in health and disease
    Yosuke Hashimoto
    Department of Surgery, Shizuoka City Shizuoka Hospital, Shizuoka 420 8630, Japan
    J Pathol 224:289-300. 2011
    ..We discuss the mechanisms by which fascins contribute to disease pathologies, especially cancer, where fascin-1 is emerging as a novel therapeutic target in carcinoma metastasis...
  37. ncbi RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion
    Esther M Lafuente
    Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:585-95. 2004
    ..RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics...
  38. pmc A distinctive role for focal adhesion proteins in three-dimensional cell motility
    Stephanie I Fraley
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Nat Cell Biol 12:598-604. 2010
    ..This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3D matrix...
  39. pmc A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis
    Ulrike Philippar
    Massachusetts Institute of Technology, Koch Institute, Cambridge, MA 02139, USA
    Dev Cell 15:813-28. 2008
    ..Upregulation of Mena(INV) could therefore enable tumor cells to invade in response to otherwise benign EGF stimulus levels...
  40. ncbi The role of actin, fimbrin and endocytosis in growth of hyphae in Aspergillus nidulans
    Srijana Upadhyay
    Program for the Biology of Filamentous Fungi, Department of Plant Pathology and Microbiology, Texas A and M University, 2132 TAMU, College Station, TX 77843, USA
    Mol Microbiol 68:690-705. 2008
    ..Endocytosis was severely impaired in apolar fimA disruption cells. Our data support a novel apical recycling model which indicates a critical role for actin patch-mediated endocytosis to maintain polarized growth at the apex...
  41. pmc Alpha-parvin controls vascular mural cell recruitment to vessel wall by regulating RhoA/ROCK signalling
    Eloi Montanez
    Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany
    EMBO J 28:3132-44. 2009
    ..Our findings show that alpha-pv represents an essential adhesion checkpoint that controls RhoA/ROCK-mediated contractility in vSMCs...
  42. ncbi Cofilin promotes actin polymerization and defines the direction of cell motility
    Mousumi Ghosh
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 304:743-6. 2004
    ..We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor...
  43. ncbi Enhanced expression of Iba1, ionized calcium-binding adapter molecule 1, after transient focal cerebral ischemia in rat brain
    D Ito
    Department of Neurology, School of Medicine, Keio University, Tokyo, Japan
    Stroke 32:1208-15. 2001
    ..In the present study we examined time-dependent Iba1 expression after transient middle cerebral artery occlusion and characterized microglial activation in various brain regions...
  44. pmc A conserved mechanism for Bni1- and mDia1-induced actin assembly and dual regulation of Bni1 by Bud6 and profilin
    James B Moseley
    Department of Biology and The Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Biol Cell 15:896-907. 2004
    ..From these results, we propose a model in which Bni1 FH2 dimers nucleate and processively cap the elongating barbed end of the actin filament, and Bud6 and profilin generate a local flux of ATP-actin monomers to promote actin assembly...
  45. pmc The tip growth apparatus of Aspergillus nidulans
    Naimeh Taheri-Talesh
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 19:1439-49. 2008
    ..Microtubules are important for delivering vesicles to the tip area and for holding the tip growth apparatus in position...
  46. ncbi Actin-Capping Protein and the Hippo pathway regulate F-actin and tissue growth in Drosophila
    Beatriz García Fernández
    Instituto Gulbenkian de Ciencia, Rua da Quinta Grande 6, P 2780 156 Oeiras, Portugal
    Development 138:2337-46. 2011
    ..Taken together, these findings indicate a novel interplay between Hippo pathway activity and actin filament dynamics that is essential for normal growth control...
  47. ncbi Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin
    S Naisbitt
    Howard Hughes Medical Institute, Department of Neurobiology, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA
    Neuron 23:569-82. 1999
    ..Shank may function as a scaffold protein in the PSD, potentially cross-linking NMDA receptor/PSD-95 complexes and coupling them to regulators of the actin cytoskeleton...
  48. ncbi Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin
    Ryusuke Niwa
    Department of Molecular Genetics, The Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Cell 108:233-46. 2002
    ..Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo...
  49. pmc Roles of Hof1p, Bni1p, Bnr1p, and myo1p in cytokinesis in Saccharomyces cerevisiae
    E A Vallen
    Department of Biology, Swarthmore College, Swarthmore, Pennsylvania 19081, USA
    Mol Biol Cell 11:593-611. 2000
    ..In bni1Delta cells that lack the actomyosin contraction, septum formation is often slow and asymmetric, suggesting that actomyosin contraction may provide directionality for efficient septum formation...
  50. ncbi Nesprins LINC the nucleus and cytoskeleton
    Jason A Mellad
    BHF Centre of Research Excellence, Division of Cardiovascular Medicine, King s College London, SE5 9NU, UK
    Curr Opin Cell Biol 23:47-54. 2011
    ..Herein we review recent advances in our understanding of how nesprins couple to various filamentous systems within the cell and emphasise the importance of both KASH and KASH-less nesprin isoforms in these interactions...
  51. ncbi Ezrin/radixin/moesin: versatile controllers of signaling molecules and of the cortical cytoskeleton
    Verena Niggli
    Department of Pathology, University of Bern, Murtenstr 31, CH 3010 Bern, Switzerland
    Int J Biochem Cell Biol 40:344-9. 2008
    ..They are involved in cell membrane organization, cell migration, phagocytosis and apoptosis, and may also play cell-specific roles in tumor progression. Their precise involvement in these processes has yet to be elucidated...
  52. ncbi Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics
    F B Gertler
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Cell 87:227-39. 1996
    ..Expression of neural-enriched isoforms of Mena in fibroblasts induces the formation of abnormal F-actin-rich outgrowths, supporting a role for this protein in microfilament assembly and cell motility...
  53. ncbi Nesprins: a novel family of spectrin-repeat-containing proteins that localize to the nuclear membrane in multiple tissues
    Q Zhang
    Department of Medicine, Division of Cardiovascular Medicine, University of Cambridge, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
    J Cell Sci 114:4485-98. 2001
    ..These data and structural analogies with other proteins suggest that nesprins may function as 'dystrophins of the nucleus' to maintain nuclear organization and structural integrity...
  54. pmc Effect of mutation type and location on clinical outcome in 1,013 probands with Marfan syndrome or related phenotypes and FBN1 mutations: an international study
    L Faivre
    Centre de Genetique, Centre Hospitalier Universitaire, Dijon, France
    Am J Hum Genet 81:454-66. 2007
    ..Exon 24-32 mutations define a high-risk group for cardiac manifestations associated with severe prognosis at all ages...
  55. pmc ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity
    Xiaoyang Wu
    Howard Hughes Medical Institute and Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10065, USA
    Cell 135:137-48. 2008
    ..Our findings provide insight into the functions of this important cytoskeletal crosslinking protein in regulating dynamic interactions between MTs and F-actin to sustain directional cell movement...
  56. ncbi Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics
    Matthias Krause
    Department of Biology and Center for Cancer Research, MIT, Cambridge, MA 02139, USA
    Dev Cell 7:571-83. 2004
    ..These phenotypes are more severe than loss of Ena/VASP, suggesting that Lpd regulates other effectors of the actin cytoskeleton in addition to Ena/VASP...
  57. ncbi Galectin-3 increases gastric cancer cell motility by up-regulating fascin-1 expression
    Seok Jun Kim
    Gastric Cancer Branch, Division of Translational and Clinical Research I, National Cancer Center Research Institute and Hospital, Jungbalsan ro, Ilsandong gu, Goyang si, Gyeonggi Do, Republic of Korea
    Gastroenterology 138:1035-45.e1-2. 2010
    ..Galectin-3 induces cytoskeletal remodeling to increase cell motility, but the mechanisms of this process are not understood. We investigated the effects of galectin-3 on fascin-1, an actin-bundling protein...
  58. ncbi Cardiovascular manifestations in men and women carrying a FBN1 mutation
    Delphine Detaint
    AP HP, Hopital Bichat, Consultation pluridisciplinaire Syndrome de Marfan et apparentés, Paris F 75018, France
    Eur Heart J 31:2223-9. 2010
    ..This study evaluates the cardiovascular (CV) risk associated with the discovery of a fibrillin-1 (FBN1) mutation...
  59. pmc Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
    Maria Nemethova
    Institute of Molecular Biotechnology, Austrian Academy of Sciences, Vienna 1030, Austria
    J Cell Biol 180:1233-44. 2008
    ..Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction...
  60. ncbi Functions of actin in endocytosis
    Alastair S Robertson
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
    Cell Mol Life Sci 66:2049-65. 2009
    ..Our discussion is limited to endocytosis involving clathrin as other types of endocytosis have not been demonstrated in yeast...
  61. pmc Involvement of CYR61 and CTGF in the fascin-mediated proliferation and invasiveness of esophageal squamous cell carcinomas cells
    Jian Jun Xie
    Department of Biochemistry and Molecular Biology, The Key Immunopathology Laboratory of Guangdong Province, Medical College of Shantou University, Shantou 515041, China
    Am J Pathol 176:939-51. 2010
    ..Taken together, we propose that fascin regulates the proliferation and invasiveness of ESCC cells by modulating the expression of CTGF and CYR61 via TGF-beta pathway...
  62. pmc Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion
    Yingjie Xu
    Lady Davis Institute for Medical Research, Jewish General Hospital, Faculty of Medicine, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Exp Med 207:2421-37. 2010
    ..These results document a regulation of FA dynamics by FLNa in breast cancer cells...
  63. pmc VASP is a processive actin polymerase that requires monomeric actin for barbed end association
    Scott D Hansen
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco School of Medicine, San Francisco, CA 94158, USA
    J Cell Biol 191:571-84. 2010
    ..Based on our observations, we propose a mechanism that accounts for all three binding modes and provides a model for how VASP promotes actin filament assembly...
  64. pmc Identification and characterization of a small molecule inhibitor of formin-mediated actin assembly
    Syed A Rizvi
    Department of Chemistry, The University of Chicago, IL 60637, USA
    Chem Biol 16:1158-68. 2009
    ..Furthermore, low micromolar concentrations of SMIFH2 disrupt formin-dependent, but not Arp2/3 complex-dependent, actin cytoskeletal structures in fission yeast and mammalian NIH 3T3 fibroblasts...
  65. pmc Filopodia and adhesion in cancer cell motility
    Antti Arjonen
    Medical Biotechnology VTT Technical Research Centre of Finland
    Cell Adh Migr 5:421-30. 2011
    ..These data implicated that several different filopodia inducing genes may contribute in a collective manner to cancer progression and the high metastasis rates associated with basal-type breast carcinomas...
  66. ncbi cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets
    E Butt
    Medizinische Universitatsklinik, Klinische Biochemie und Pathobiochemie, Federal Republic of Germany
    J Biol Chem 269:14509-17. 1994
    ....
  67. pmc Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines
    Cristian M Ortiz
    Department of Pathology, Valencia University, Hospital General Universitario de Valencia, Avenida tres cruces N degrees 2, CP 46014, Valencia, Spain
    Diagn Pathol 5:41. 2010
    ..Using a stable knockdown cell line, we revealed the effect of fascin on cell growth, cell adhesion and tumor formation...
  68. pmc Disruption of SM22 promotes inflammation after artery injury via nuclear factor kappaB activation
    Jianbin Shen
    Department of Internal Medicine, Wayne State University, Detroit, MI 48201, USA
    Circ Res 106:1351-62. 2010
    ..Abolishing SM22 in apolipoprotein E knockout mice accelerates atherogenesis. However, it is unclear whether SM22 disruption independently promotes arterial inflammation...
  69. pmc Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPK1 and MAPK2 at promyelocytic leukemia protein nuclear bodies and act to regulate smooth muscle cell proliferation
    Derek T Warren
    British Heart Foundation Centre, Division of Cardiovascular Medicine, King s College London, London SE5 9NU, United Kingdom
    J Biol Chem 285:1311-20. 2010
    ..Taken together, this study suggests that nesprin-2 is a novel intranuclear scaffold, essential for nuclear ERK1/2 signaling fidelity and cell cycle progression...
  70. pmc Deconstructing the cadherin-catenin-actin complex
    Soichiro Yamada
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 123:889-901. 2005
    ..These results suggest that the linkage between the cadherin-catenin complex and actin filaments is more dynamic than previously appreciated...
  71. ncbi Transgelin promotes migration and invasion of cancer stem cells
    Eun Kyung Lee
    School of Life Sciences and Biotechnology, Korea University, Seoul, Korea
    J Proteome Res 9:5108-17. 2010
    ..Taken together, these results indicate that the metastatic potential of CSCs arises from highly expressed Transgelin...
  72. ncbi Formin is a processive motor that requires profilin to accelerate actin assembly and associated ATP hydrolysis
    Stephane Romero
    Laboratoire d Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Cell 119:419-29. 2004
    ..We successfully reconstitute formin-induced motility in vitro, demonstrating that this mechanism accounts for the puzzlingly rapid formin-induced actin processes observed in vivo...
  73. ncbi Proteomic analysis of fibrillin-rich microfibrils
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Manchester, UK
    Proteomics 6:111-22. 2006
    ..This first MS analysis of a purified extracellular matrix assembly has provided new insights into microfibril composition and fibrillin-1 organisation within them...
  74. ncbi Regulation of E-cadherin endocytosis by nectin through afadin, Rap1, and p120ctn
    Takashi Hoshino
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Osaka 565 0871, Japan
    J Biol Chem 280:24095-103. 2005
    ....
  75. ncbi Enaptin, a giant actin-binding protein, is an element of the nuclear membrane and the actin cytoskeleton
    V C Padmakumar
    Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, FRG Germany
    Exp Cell Res 295:330-9. 2004
    ..With the discovery of the actin-binding properties of Enaptin and the highly homologous Nuance, we define a family of proteins that integrate the cytoskeleton with the nucleoskeleton...
  76. ncbi bottleneck acts as a regulator of the microfilament network governing cellularization of the Drosophila embryo
    E D Schejter
    Department of Molecular Biology, Princeton University, New Jersey 08544
    Cell 75:373-85. 1993
    ..The expression pattern and mutant phenotype of bnk suggest a direct role for this element in regulation of the dynamic restructuring of the actin-based cytoskeleton of cellularizing Drosophila embryos...
  77. ncbi Nectin and afadin: novel organizers of intercellular junctions
    Yoshimi Takai
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Cell Sci 116:17-27. 2003
    ..The adhesion system is furthermore involved in the formation of synapses in neurons and the organization of heterotypic junctions between Sertoli cells and spermatids in the testis...
  78. ncbi Recruitment of the endosomal WASH complex is mediated by the extended 'tail' of Fam21 binding to the retromer protein Vps35
    Michael E Harbour
    Cambridge Institute for Medical Research Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 0XY, UK
    Biochem J 442:209-20. 2012
    ..Additionally, overexpression of the Fam21-tail results in cell-spreading defects, implicating the activity of the WASH complex in regulating the mobilization of membrane into the endosome-to-cell surface pathway...
  79. ncbi Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis
    J M Kaplan
    Renal and Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Nat Genet 24:251-6. 2000
    ..Our results have implications for understanding the role of the cytoskeleton in the pathophysiology of kidney disease and may lead to a better understanding of the genetic basis of susceptibility to kidney damage...
  80. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
    ..In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei...
  81. pmc Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division
    Sybille Pfender
    Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
    Curr Biol 21:955-60. 2011
    ..This study not only reveals how Spire1 and Spire2 drive two critical steps of asymmetric oocyte division, but it also uncovers the first physiological function of Spire-type actin nucleators in vertebrates...
  82. ncbi NUANCE, a giant protein connecting the nucleus and actin cytoskeleton
    Yen Yi Zhen
    Institute for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany
    J Cell Sci 115:3207-22. 2002
    ..The C-terminal transmembrane domain is responsible for the targeting the nuclear envelope. Thus, NUANCE is the first alpha-actinin-related protein that has the potential to link the microfilament system with the nucleus...
  83. ncbi Role of actin polymerization and actin cables in actin-patch movement in Schizosaccharomyces pombe
    R J Pelham
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, USA
    Nat Cell Biol 3:235-44. 2001
    ..Our studies give rise to a model in which actin patches are centres for actin polymerization that drive their own movement on actin cables using Arp2/3-based actin polymerization...
  84. ncbi Prognostic significance of basal-like phenotype and fascin expression in node-negative invasive breast carcinomas
    Socorro Maria Rodriguez-Pinilla
    Breast and Gynecological Cancer Group, Molecular Pathology Programme and Department of Human Genetics, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
    Clin Cancer Res 12:1533-9. 2006
    ..The aim of this study was to determine whether basal-like phenotype and fascin were related in both sporadic and familial tumors and with prognosis in node-negative sporadic breast cancers...
  85. pmc Identification of filamin as a novel ligand for caveolin-1: evidence for the organization of caveolin-1-associated membrane domains by the actin cytoskeleton
    M Stahlhut
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    Mol Biol Cell 11:325-37. 2000
    ..The actin network, therefore, seems to be directly involved in the spatial organization of caveolin-1-associated membrane domains...
  86. ncbi WASH, WHAMM and JMY: regulation of Arp2/3 complex and beyond
    Klemens Rottner
    Cytoskeleton Dynamics Group, Helmholtz Centre for Infection Research, Inhoffen Strasse 7, Braunschweig, Germany
    Trends Cell Biol 20:650-61. 2010
    ..Uncovering the physiological relevance of these unique activities will require concerted efforts from multiple disciplines, and is sure to impact our understanding of how the cytoskeleton controls so many dynamic subcellular events...
  87. pmc Dissecting BAR domain function in the yeast Amphiphysins Rvs161 and Rvs167 during endocytosis
    Ji Young Youn
    Department of Molecular Genetics, Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S3E1, Canada
    Mol Biol Cell 21:3054-69. 2010
    ..We show that inappropriate regulation of complex sphingolipid and phosphoinositide levels in the membrane can impinge on Rvs function, highlighting the relationship between membrane components and N-BAR-domain proteins in vivo...
  88. ncbi Visualization of F-actin localization and dynamics with live cell markers in Neurospora crassa
    Diego L Delgado-Alvarez
    Departamento de Microbiologia, Centro de Investigación Científica y de Educación Superior de Ensenada CICESE, Ensenada, B C, Mexico
    Fungal Genet Biol 47:573-86. 2010
    ..Moreover, Lifeact-GFP marked the broadest spectrum of F-actin structures; it may serve as a global live cell marker for F-actin in filamentous fungi...
  89. ncbi Role of ANC-1 in tethering nuclei to the actin cytoskeleton
    Daniel A Starr
    Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Science 298:406-9. 2002
    ..Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm...
  90. ncbi Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia
    K Ohsawa
    Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo 187 8502, Japan
    J Cell Sci 113:3073-84. 2000
    ..These observations suggest that Iba1 is involved in Rac and calcium signaling pathways...
  91. pmc Get to grips: steering local actin dynamics with IQGAPs
    Dominique T Brandt
    Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany
    EMBO Rep 8:1019-23. 2007
    ....
  92. pmc Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family
    D Reczek
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Cell Biol 139:169-79. 1997
    ..Since PDZ domains are known to mediate associations with integral membrane proteins, one mode of membrane attachment of ezrin is likely to be mediated through EBP50...
  93. pmc Differential interactions of the formins INF2, mDia1, and mDia2 with microtubules
    Jérémie Gaillard
    CEA, iRTSV, Laboratoire Physiologie Cellulaire and Végétale, CNRS, UMR5168, Universite Joseph Fourier Grenoble I, F 38054 Grenoble, France
    Mol Biol Cell 22:4575-87. 2011
    ..These differences in interactions with microtubules and actin suggest differential function in cellular processes requiring both cytoskeletal elements...
  94. pmc Mutations in the TGFβ binding-protein-like domain 5 of FBN1 are responsible for acromicric and geleophysic dysplasias
    Carine Le Goff
    Department of Genetics, Universite Paris Descartes, Unité Institut National de la Santé et de la Recherche Médicale, Hopital Necker Enfants Malades, Paris, France
    Am J Hum Genet 89:7-14. 2011
    ..Although enhanced TGFβ signaling caused by FBN1 mutations can trigger either Marfan syndrome or GD and AD, our findings support the fact that TB5 mutations in FBN1 are responsible for short stature phenotypes...
  95. ncbi Plakins in development and disease
    Arnoud Sonnenberg
    Division of Cell Biology, The Netherlands Cancer Inst, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Exp Cell Res 313:2189-203. 2007
    ..We will also describe the relationship of these two proteins to the invertebrate plakins, shortstop (shot) in Drosophila and VAB-10 in C. elegans. Finally, we will describe an unusual mammalian plakin, called epiplakin...
  96. pmc Lamin A/C-dependent localization of Nesprin-2, a giant scaffolder at the nuclear envelope
    Thorsten Libotte
    Center for Biochemistry, Medical Faculty, University of Cologne, 50931 Cologne, Germany
    Mol Biol Cell 16:3411-24. 2005
    ..Our data imply a scaffolding function of Nesprin-2 at the nuclear membrane and suggest a potential involvement of this multi-isomeric protein in human disease...
  97. pmc Peripheral nerve demyelination caused by a mutant Rho GTPase guanine nucleotide exchange factor, frabin/FGD4
    Claudia Stendel
    Institute of Cell Biology, ETH Zurich, Schafmattstrasse 18, CH 8093 Zurich, Switzerland
    Am J Hum Genet 81:158-64. 2007
    ..These data, together with the ability of frabin to induce Cdc42-mediated cell-shape changes in transfected Schwann cells, suggest that Rho GTPase signaling is essential for proper myelination of the peripheral nervous system...
  98. pmc The role of fascin in the migration and invasiveness of malignant glioma cells
    Jeong Hyun Hwang
    Department of Neurosurgery, School of Medicine, Kyungpook National University, Daegu, South Korea
    Neoplasia 10:149-59. 2008
    ..Finally, we show a decreased invasiveness of U251-GFP cells by fascin knockdown in an ex vivo rat brain slice model system. This is the first study to demonstrate a role for fascin in glioma cell morphology, motility, and invasiveness...
  99. pmc Fibronectin matrix assembly requires distinct contributions from Rho kinases I and -II
    Atsuko Yoneda
    Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 18:66-75. 2007
    ..Together, two rho kinases contribute to fibronectin matrix assembly in a different manner and cortical myosin II-driven contractility, but not stress fibers, may be critical in this activity...
  100. ncbi Roles of fascin in human carcinoma motility and signaling: prospects for a novel biomarker?
    Yosuke Hashimoto
    Department of Cell Biology, Lerner Research Institute, NC1 10, Cleveland Clinic Foundation, OH 44195, USA
    Int J Biochem Cell Biol 37:1787-804. 2005
    ..Here, we discuss the evidence that fascin is upregulated in carcinomas, its contributions to carcinoma cell behaviour and its potential as a candidate novel biomarker or therapeutic target...
  101. pmc Migrastatin analogues target fascin to block tumour metastasis
    Lin Chen
    Department of Physiology, Cornell University Weill Medical College, New York, New York 10065, USA
    Nature 464:1062-6. 2010
    ..Our data demonstrate that actin cytoskeletal proteins such as fascin can be explored as new molecular targets for cancer treatment, in a similar manner to the microtubule protein tubulin...

Research Grants69

  1. ROLES OF MICROFILAMENT PROTEINS IN NEOPLASIA/METASTASIS
    John Leavitt; Fiscal Year: 1993
    ....
  2. Motor Systems Supporting Pavlovian Conditioning
    Terry Crow; Fiscal Year: 2008
    ..These studies will help to elucidate general principles underlying the organization of polysensory neural systems generating behavior and their modification by learning. ..
  3. Skyscan 1172 ex-vivo microComputed Tomography System
    Francesco Ramirez; Fiscal Year: 2007
    ..Acquisition of this equipment will enable our institutions to engage in biomedical research highly relevant to pathophysiological processes and animal models of human diseases. [unreadable] [unreadable] [unreadable]..
  4. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  5. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY YW BOURGUIGNON; Fiscal Year: 2010
    ....
  6. Fascin Protrusions in ECM-Cytoskeletal Networking
    Josephine Adams; Fiscal Year: 2007
    ....
  7. PPAR gamma Signaling and Lung Fibroblast Transdifferentiation
    Virender Rehan; Fiscal Year: 2009
    ..abstract_text> ..
  8. EM & TIRF analysis of Arp2/3 complex and actin assembly
    BRUCE GOODE; Fiscal Year: 2008
    ..The PI will be directly involved in the integration of TIRF microscopy and single particle imaging into his research program. ..
  9. "The Role of HDAC3 in Cardiac Growth and Development"
    Lazaros Kochilas; Fiscal Year: 2007
    ..They will also use selected molecular markers to dissect extracardiac processes relevant to cardiac development that are potentially affected in the hdac3 deficient embryos. [unreadable] [unreadable] [unreadable]..
  10. Pathogenesis and Therapy of Marfan Vascular Disease
    Daniel Judge; Fiscal Year: 2007
    ..Dr. Judge's training will be based in the School of Public Health at Johns Hopkins, and he will be advised by a committee of experts to create a fruitful environment for the development of an independent clinical scientist. ..
  11. Delta-Catenin Cleavage by Presenilin and Synaptic Remodeling
    Qun Lu; Fiscal Year: 2006
    ..It will also lay the foundation for future R01 investigation of the roles of delta-catenin proteolytic fragments in AD pathogenesis. ..
  12. CONFORMATIONAL STABILITY OF GLOBULAR PROTEINS
    CARLOS PACE; Fiscal Year: 2006
    ..To gain a better understanding of protein solubility, we will study the effect of single changes in the amino acid sequence of RNase Sa on the solubility of the folded and unfolded states of the protein. ..
  13. Protein ubiquitination based neuroprotection for stroke
    Robert Meller; Fiscal Year: 2006
    ....
  14. The Biology and Function of BMP-7 in the Kidney
    Raghu Kalluri; Fiscal Year: 2007
    ..The physiological role of BMP-7 in kidney health and pathology will be established. These studies will directly benefit the development of this molecule in the clinic as a drug for patients with kidney fibrosis. ..
  15. Monotonously Ordered Peptide Elements
    Fred Stevens; Fiscal Year: 2008
    ..In general, the proposed studies are designed to begin to evaluate the prospects of future studies to determine the physiological roles of particular MOPE-class proteins. [unreadable] [unreadable] [unreadable]..
  16. Cannabinoids and Neuroimmune Interactions
    Edgar Alfonso Romero Sandoval; Fiscal Year: 2010
    ..The development of new drug strategies to treat acute and chronic pain safely and effectively will result in an invaluable improvement for millions of patients. ..
  17. Role of Vav family proteins in cell signaling and cancer
    XOSE BUSTELO; Fiscal Year: 2009
    ..abstract_text> ..
  18. Role of Fibroblasts in Cancer
    Raghu Kalluri; Fiscal Year: 2010
    ..The role of such fibroblasts in cancer progression and metastasis is unknown. This proposal addresses the role of fibroblasts in facilitating metastasis. ..
  19. EFFECTS OF MORPHINE AND ANALOGUES ON CELL METABOLISM
    Eric Simon; Fiscal Year: 2007
    ..This should provide new information and also serve to validate, or find the flaws in, the widely used model of transfected cells expressing non-physiologically high levels of opioid receptors. ..
  20. Design of a light-switched, genetically-encoded regulator of actin assembly
    BRUCE GOODE; Fiscal Year: 2008
    ..We are proposing to develop new research tools to better understand the function of this protein and to help develop cures for the diseases caused by its malfunction. [unreadable] [unreadable] [unreadable]..
  21. Mechanisms of Cardiac Regeneration
    DINENDER SINGLA; Fiscal Year: 2008
    ..The results from the proposed projects will have long-term implications for future cell-based therapy to treat heart disease (s) using embryonic stem cells or potentially other donor cells. [unreadable] [unreadable] [unreadable]..
  22. Genetic Investigation of Arrhythmogenic Right Ventricular Dysplasia
    Daniel Judge; Fiscal Year: 2008
    ..A more efficient type of genetic analysis will be tested. This will lead to better understanding of genetic susceptibility to sudden death from heart disease. [unreadable] [unreadable] [unreadable]..
  23. Architecture of Basal Fiber Components in the Lens
    KRISTIN AL GHOUL; Fiscal Year: 2008
    ..The proposed studies represent a logical extension of our previous work and will advance the field by defining the molecular organization of the BMC during normal and abnormal fiber end migration. ..
  24. Seizure-Induced Neurogenesis In The Dentate Gyrus
    CHARLES RIBAK; Fiscal Year: 2008
    ..Furthermore we will determine the effects of irradiation on the populations of granule cells with HBDs and hilar EGCs. This knowledge may in turn be used to develop rational therapeutics to block spontaneous seizure development. ..
  25. Thymosin beta 4 and Corneal Wound Healing
    Gabriel Sosne; Fiscal Year: 2005
    ..abstract_text> ..
  26. REGULATION OF INTESTINAL EPITHELIAL CELL PROLIFERATION
    John Lynch; Fiscal Year: 2004
    ..It will also enhance our understanding of the events leading to dysregulation of proliferation and human colon cancers. ..
  27. The Promoter or A Prostate Specific Gene, Cten
    SU LO; Fiscal Year: 2004
    ....
  28. Opioid Antagonists with Morphine for Neuropathic Pain
    LINDSAY BURNS; Fiscal Year: 2004
    ..Moreover, analgesia for neuropathic pain is a large unmet medical need, and there is strong rationale for this investigation. ..
  29. Regulation of the Actin Cytoskeleton in Dendric Spines
    Peter Penzes; Fiscal Year: 2002
    ..serine/threonine phosphorylation, and generate initial information about the types of protein kinases involved. Together, these experiments will provide a basis for future studies of regulation of kalirin-7 and spine morphogenesis. ..
  30. LASER SCANNING CONFOCAL IMAGING SYSTEM
    Liza Pon; Fiscal Year: 2003
    ..I. As an integral component of our Optical Microscopy Facility, the instrument will be accessible to members of the user group and others throughout the University, promoting collaboration and exchange of ideas. ..
  31. CELLULAR CONTEXT OF NF-KB ACTIVATION
    Brian Seed; Fiscal Year: 2004
    ..In addition, we propose to gain insight into the natural physiology of NF-kB activation by studying the contexts that lead to reporter gene activation in vivo. ..
  32. CO POISONING IN THE CONTEXT OF A REPERFUSION INJURY
    Stephen Thom; Fiscal Year: 2003
    ..The research plan is aimed to test the hypothesis that immune responses and the associated oxidative stress cause neurological injuries. ..
  33. TARGETING OF PROTEINS INTO PEROXISOMES
    Suresh Subramani; Fiscal Year: 2003
    ..To investigate the targeting and topology of peroxisomal membrane proteins 6. To study the role of the P. pastoris PAS1 and PAS5 proteins in peroxisome biogenesis ..
  34. Auditory Cortex: Regional Pathology In Schizophrenia
    Robert Sweet; Fiscal Year: 2003
    ..abstract_text> ..
  35. MOLECULAR MECHANISMS OF ETHANOL INDUCED LIVER FIBROGENES
    Francesco Ramirez; Fiscal Year: 2002
    ..abstract_text> ..
  36. Ultra high field 900 MHz NMR spectrometer
    Jun Qin; Fiscal Year: 2003
    ..In turn, this will increase the critical mass of expertise in the region, which will promote the development of cutting edge biological research and associated high technology industry in the mid-west. ..
  37. REGULATION OF ANGIOGENESIS BY MICROENVIRONMENTAL CUES
    Christopher Chen; Fiscal Year: 2001
    ..In this manner, he will seek to determine the ability of matrix architecture to regulate capillary cell behavior and, thus, provide a rational basis for the design of the engineered matrices to promote tissue vascularization. ..
  38. Conference on Wiring and Plasticity of Nervous Systems
    Thomas Pollard; Fiscal Year: 2002
    ..We are confident that mutually beneficial insights will emerge from learning about each others work. ..
  39. INSULIN RESISTANCE IN PCOS--SEQUELAE AND TREATMENT
    RICHARD LEGRO; Fiscal Year: 2005
    ..Improving insulin action through diet and exercise, with and without weight loss, will result in lowered circulating insulin levels, lowered androgens and increased ovulatory frequency rate in PCOS women. ..
  40. BIOCHEMICAL RESEARCH ON OPIOID RECEPTORS
    Eric Simon; Fiscal Year: 2003
    ..In the final aim, aspects of signal transduction will be studied, including identification of receptor regions involved in G protein activation. ..
  41. RECOGNITION AND SORTING OF GLYCOPROTEINS
    Michael Roth; Fiscal Year: 2002
    ....
  42. EFFERENT INNERVATION OF IDENTIFIED VESTIBULAR AFFERENTS
    Anna Lysakowski; Fiscal Year: 2008
    ..The intent is to produce a body of morphological, neurochemical and cell biological knowledge from which physiologically and pharmacologically testable hypotheses can be derived. [unreadable] [unreadable] [unreadable]..
  43. BIOLOGY OF HUMAN INTRAEPITHELIAL LYMPHOCYTES
    Ellen Ebert; Fiscal Year: 2002
    ..These studies will determine the mechanism of IEL chemotaxis toward secreted products of ECs, why IL-15 is more potent than IL-2 in inducing LAK activity by IELs, and how IL-10 and IL-12 augment this activity. ..
  44. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
    ....
  45. NEW TARGETED THERAPIES FOR ISCHEMIC MITRAL REGURGITATION
    Robert Levine; Fiscal Year: 2008
    ..The overall goal is to provide a sound basis for more consistent and effective repair of ischemic MR to reduce this frequent stimulus to heart failure in patients. [unreadable] [unreadable]..
  46. Regulation of alpha2A-AR trafficking/signaling by different agonists in neurons
    Qin Wang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  47. delta-Catenin and Cell-Cell Adhesion in Prostate Cancer
    Qun Lu; Fiscal Year: 2010
    ..Understanding the posttranslational modifications that control 8-catenin functions will unravel mechanisms by which S-catenin modulates gene expression and promotes CaP in vivo. ..
  48. COLLAGEN XI ISOFORMS IN CARTILAGE AND BONE PHYSIOLOGY
    Francesco Ramirez; Fiscal Year: 2001
    ..It is suggested (by the applicant) that this information will advance our understanding of the pathogenesis of disorders that impair skeletal function. ..
  49. International Meeting--Epithelial /Mesenchymal Tranition
    Raghu Kalluri; Fiscal Year: 2005
    ..abstract_text> ..
  50. Actin Organization and Polarization
    Liza Pon; Fiscal Year: 2005
    ..The studies proposed are designed to fill that gap. ..
  51. SIGNAL TRANSDUCTION EVENTS REGULATED BY INTEGRINS
    Joan Brugge; Fiscal Year: 2003
    ....
  52. Force Probe Investigations of NCAM Adhesion
    Deborah Leckband; Fiscal Year: 2004
    ....
  53. Role of Rho Kinase in Aqueous Humor Outflow Pathway
    P Rao; Fiscal Year: 2004
    ..Identification of specific signaling mechanisms regulating function of outflow pathway tissues could enable the design of target-specific approaches to the treatment of glaucoma. ..
  54. Matrix Microenvironment and Liver Fibrosis
    Raghu Kalluri; Fiscal Year: 2007
    ..Therefore, this application is well poised to identify a unique mechanism of liver fibrosis and also study a therapeutic agent, which works against this mechanism and inhibits progression of liver disease. ..
  55. AUTOSOMAL DOMINANT FOCAL SEGMENTAL GLOMERULOSCLEROSIS
    Michelle Winn; Fiscal Year: 2004
    ..Information derived from this research will also allow a better understanding of the pathogenesis of nonhereditary forms of FSGS and may provide insights regarding management of the more common idiopathic forms of this disorder. ..
  56. HDAd-mediated gene therapy for hemophilia B
    Nicola Brunetti Pierri; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  57. Hereditary Hemolytic Anemia: A Proteomic Approach
    JASON WOODEN; Fiscal Year: 2007
    ..We hope that a deeper understanding of protein-protein interactions within the RBC can provide new insights into inherited hemolytic anemia. ..
  58. eNOS-Protein Interaction in the Penis
    Biljana Musicki; Fiscal Year: 2007
    ..At the same time, it may suggest a specific eNOS regulatory pathway as a possible new molecular target for treating ED. [unreadable] [unreadable] [unreadable]..
  59. SYNAPTOPODIN: BIOGENESIS & PLASTICITY OF SPINE APPARATUS
    Peter Mundel; Fiscal Year: 2008
    ..This should in the long-term enable us to develop novel therapies that tackle learning deficiencies and memory loss by modulating the expression of synaptopodin thereby promoting the cellular plasticity of the dendritic spine apparatus. ..
  60. SACRAL CREST CELLS AND ENTERIC NEURODEVELOPMENT
    Raj Kapur; Fiscal Year: 2005
    ..The results of this study will further our understanding of normal enteric neurodevelopment and congenital disorders of intestinal motility. ..
  61. Functions of the Epithelial Septate Junction
    Richard Fehon; Fiscal Year: 2009
    ..abstract_text> ..
  62. SPATIAL AND TEMPORAL REGULATION OF PROTEIN KINASE D
    Osvaldo Rey; Fiscal Year: 2007
    ..Our hypothesis is that the kinase activity of PKC regulates PKD by coordinating its catalytic activation and intracellular localization. [unreadable] [unreadable]..
  63. Molecular Mechanisms of Mesangial Sclerosis
    Christine Abrass; Fiscal Year: 2009
    ..Understanding transcriptional control of LN isoform expression will add new insights into the role that LN plays in normal glomerulogenesis and that becomes disordered in glomerular disease. ..
  64. Function of VirK and MsbB2 in Shigella pathogenesis
    MARCIA GOLDBERG; Fiscal Year: 2006
    ..These exploratory studies are highly likely to result in specific testable hypotheses that are relevant to disease. [unreadable] [unreadable] [unreadable]..
  65. Autotrasporter periplasmic chaperones in Shigella
    MARCIA GOLDBERG; Fiscal Year: 2005
    ..These exploratory studies are highly likely to result in additional specific testable hypotheses relevant to disease caused by this Category B Priority Pathogen. ..
  66. Alveolization in Fibrillin-1 Defective Mice
    Enid Neptune; Fiscal Year: 2005
    ..abstract_text> ..