electron transport complex iii

Summary

Summary: A multisubunit enzyme complex that contains CYTOCHROME B GROUP; CYTOCHROME C1; and iron-sulfur centers. It catalyzes the oxidation of ubiquinol to UBIQUINONE, and transfers the electrons to CYTOCHROME C. In MITOCHONDRIA the redox reaction is coupled to the transport of PROTONS across the inner mitochondrial membrane.

Top Publications

  1. ncbi Production of reactive oxygen species by mitochondria: central role of complex III
    Qun Chen
    Department of Medicine, Division of Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:36027-31. 2003
  2. ncbi Electron transfer by domain movement in cytochrome bc1
    Z Zhang
    E O Lawrence Berkeley National Laboratory, University of California, 94720, USA
    Nature 392:677-84. 1998
  3. ncbi Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing
    Robert D Guzy
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    Cell Metab 1:401-8. 2005
  4. ncbi Two modes of mitochondrial dysfunction lead independently to lifespan extension in Caenorhabditis elegans
    Wen Yang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Aging Cell 9:433-47. 2010
  5. ncbi Respiratory active mitochondrial supercomplexes
    Rebeca Acín-Pérez
    Departamento de Bioquimica, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
    Mol Cell 32:529-39. 2008
  6. ncbi Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer
    Sozanne R N Solmaz
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
    J Biol Chem 283:17542-9. 2008
  7. ncbi Acridinediones: selective and potent inhibitors of the malaria parasite mitochondrial bc1 complex
    Giancarlo A Biagini
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK
    Mol Pharmacol 73:1347-55. 2008
  8. ncbi An electronic bus bar lies in the core of cytochrome bc1
    Monika Swierczek
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Science 329:451-4. 2010
  9. ncbi Physiological diversity of mitochondrial oxidative phosphorylation
    G Benard
    INSERM U688, Physiopathologie Mitochondriale, , Bordeaux, France
    Am J Physiol Cell Physiol 291:C1172-82. 2006
  10. ncbi Energy thresholds in brain mitochondria. Potential involvement in neurodegeneration
    G P Davey
    Department of Neurochemistry, Institute of Neurology, Queen Square, London WC1N 3BG, United Kingdom
    J Biol Chem 273:12753-7. 1998

Research Grants

  1. HYDROPHOBIC ASSOCIATIONS OF MITOCHONDRIAL PROTEINS
    NEAL ROBINSON; Fiscal Year: 1993
  2. BIOENERGETICS OF THE CYTOCHROME BC1 COMPLEX
    DIANA BEATTIE; Fiscal Year: 2001
  3. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    Neal C Robinson; Fiscal Year: 2010
  4. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2009
  5. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2010
  6. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    Neal C Robinson; Fiscal Year: 2011
  7. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    NEAL ROBINSON; Fiscal Year: 2009
  8. MOLECULAR GENETICS OF THE CYTOCHROME BC1 C COMPLEX
    Fevzi Daldal; Fiscal Year: 1993
  9. Charge Transfer Reactions of the Cytochrome bc1 Complex
    Colin A Wraight; Fiscal Year: 2010
  10. Charge Transfer Reactions of the Cytochrome bc1 Complex
    COLIN WRAIGHT; Fiscal Year: 2007

Detail Information

Publications213 found, 100 shown here

  1. ncbi Production of reactive oxygen species by mitochondria: central role of complex III
    Qun Chen
    Department of Medicine, Division of Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:36027-31. 2003
    ..In mitochondria, complex III is the principal site for ROS generation during the oxidation of complex I substrates, and rotenone protects by limiting electron flow into complex III...
  2. ncbi Electron transfer by domain movement in cytochrome bc1
    Z Zhang
    E O Lawrence Berkeley National Laboratory, University of California, 94720, USA
    Nature 392:677-84. 1998
    ..Such a mechanism has not previously been observed in redox protein complexes...
  3. ncbi Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing
    Robert D Guzy
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    Cell Metab 1:401-8. 2005
    ..These results demonstrate that mitochondria function as O(2) sensors and signal hypoxic HIF-1 alpha and HIF-2 alpha stabilization by releasing ROS to the cytosol...
  4. ncbi Two modes of mitochondrial dysfunction lead independently to lifespan extension in Caenorhabditis elegans
    Wen Yang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Aging Cell 9:433-47. 2010
    ..Our results demonstrate that distinct and separable aspects of mitochondrial biology affect lifespan independently...
  5. ncbi Respiratory active mitochondrial supercomplexes
    Rebeca Acín-Pérez
    Departamento de Bioquimica, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
    Mol Cell 32:529-39. 2008
    ..Therefore, we have demonstrated the existence of a functional respirasome and propose a structural organization model that accommodates these findings...
  6. ncbi Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer
    Sozanne R N Solmaz
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
    J Biol Chem 283:17542-9. 2008
    ..Four core interactions encircle the heme cofactors surrounded by variable interactions. The core interface may be a feature to gain specificity for formation of the reactive complex...
  7. ncbi Acridinediones: selective and potent inhibitors of the malaria parasite mitochondrial bc1 complex
    Giancarlo A Biagini
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK
    Mol Pharmacol 73:1347-55. 2008
    ..falciparum bc(1) Q(o). Dihydroacridinediones represent an entirely new class of bc(1) inhibitors and the potential of these compounds as novel antimalarial drugs is discussed...
  8. ncbi An electronic bus bar lies in the core of cytochrome bc1
    Monika Swierczek
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Science 329:451-4. 2010
    ..Free and unregulated distribution of electrons acts like a molecular-scale bus bar, a design often exploited in electronics...
  9. ncbi Physiological diversity of mitochondrial oxidative phosphorylation
    G Benard
    INSERM U688, Physiopathologie Mitochondriale, , Bordeaux, France
    Am J Physiol Cell Physiol 291:C1172-82. 2006
    ..To conclude, we submitted our data to a principal component analysis that revealed three groups of tissues: muscle and heart, brain, and liver and kidney...
  10. ncbi Energy thresholds in brain mitochondria. Potential involvement in neurodegeneration
    G P Davey
    Department of Neurochemistry, Institute of Neurology, Queen Square, London WC1N 3BG, United Kingdom
    J Biol Chem 273:12753-7. 1998
    ..The implications of these findings are discussed with respect to neurodegenerative disorders and energy metabolism in the synapse...
  11. ncbi Intermonomer electron transfer between the low-potential b hemes of cytochrome bc₁
    Pascal Lanciano
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States
    Biochemistry 50:1651-63. 2011
    ..Overall findings are discussed with respect to intra- and intermonomer interactions that take place during the catalytic turnover of cyt bc(1)...
  12. ncbi Direct demonstration of half-of-the-sites reactivity in the dimeric cytochrome bc1 complex: enzyme with one inactive monomer is fully active but unable to activate the second ubiquinol oxidation site in response to ligand binding at the ubiquinone reducti
    Michela Castellani
    Institute of Biochemistry, Molecular Genetics, Goethe University and Cluster of Excellence Macromolecular Complexes Frankfurt am Main, D 60438 Frankfurt am Main, Germany
    J Biol Chem 285:502-10. 2010
    ....
  13. ncbi Threshold effect and tissue specificity. Implication for mitochondrial cytopathies
    R Rossignol
    INSERM EMI 9929, , , F 33076 Bordeaux cedex, France
    J Biol Chem 274:33426-32. 1999
    ..We propose a classification of tissues according to their type of OXPHOS response to a complex deficiency and therefore to their threshold values...
  14. ncbi Proteomic analysis of mitochondria-to-nucleus retrograde response in human cancer
    Mariola Kulawiec
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York, USA
    Cancer Biol Ther 5:967-75. 2006
    ..Our study opens an avenue for identification of retrograde proteins as potential tumor suppressors or oncogenes involved in carcinogenesis...
  15. ncbi Generation of reactive oxygen species by the mitochondrial electron transport chain
    Yuanbin Liu
    Cellular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 9062, USA
    J Neurochem 80:780-7. 2002
    ..These new insights clarify an elusive target for intervening mitochondrial ROS-related processes or diseases...
  16. ncbi Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity
    Genji Kurisu
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    Science 302:1009-14. 2003
    ..The motion of the Rieske iron-sulfur protein extrinsic domain, essential for electron transfer, must also be different in the b6f complex...
  17. ncbi Elimination of the disulfide bridge in the Rieske iron-sulfur protein allows assembly of the [2Fe-2S] cluster into the Rieske protein but damages the ubiquinol oxidation site in the cytochrome bc1 complex
    Torsten Merbitz-Zahradnik
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Biochemistry 42:13637-45. 2003
    ....
  18. ncbi The mechanism of mitochondrial superoxide production by the cytochrome bc1 complex
    Stefan Dröse
    Molecular Bioenergetics Group, Cluster of Excellence Frankfurt Macromolecular Complexes, Medical School, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    J Biol Chem 283:21649-54. 2008
    ..The resulting mechanism of ROS production offers a straightforward explanation of how the redox state of the ubiquinone pool could play a central role in mitochondrial redox signaling...
  19. ncbi Conformationally linked interaction in the cytochrome bc(1) complex between inhibitors of the Q(o) site and the Rieske iron-sulfur protein
    Edward A Berry
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA
    Biochim Biophys Acta 1807:1349-63. 2011
    ..This article is part of a Special Issue entitled: Allosteric cooperativity in respiratory proteins...
  20. ncbi CA125 and UQCRFS1 FISH studies of ovarian carcinoma
    Saori J Kaneko
    Department of Obstetrics and Gynecology, University of South Carolina School of Medicine, Columbia, SC 29203, USA
    Gynecol Oncol 90:29-36. 2003
    ..As a control we chose BACs close to the chromosome 19 centromere. One of these BACs carries the gene UQCRFS1...
  21. ncbi Ubiquinol cytochrome c reductase (UQCRFS1) gene amplification in primary breast cancer core biopsy samples
    Yoko Ohashi
    Department of Obstetrics and Gynecology, University of South Carolina School of Medicine, Columbia, SC 29203, USA
    Gynecol Oncol 93:54-8. 2004
    ..The purpose of this study is to evaluate the significance of the ubiquinol cytochrome c reductase UQCRFS1 gene amplification in primary breast cancers...
  22. ncbi Purification of a cytochrome bc-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunity of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1
    Axel Niebisch
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    J Biol Chem 278:4339-46. 2003
    ..This indicates that the attachment of both heme groups to apo-cytochrome c(1) is not only required for the activity but also for the assembly and/or stability of the bc(1) complex...
  23. ncbi The iron-sulfur cluster of the Rieske iron-sulfur protein functions as a proton-exiting gate in the cytochrome bc(1) complex
    Buddha Gurung
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:24895-902. 2005
    ..It was speculated that in the normal catalytic cycle of the bc(1) complex, the [2Fe-2S] cluster may function as a proton-exiting gate...
  24. ncbi The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc(1) complexes
    Tiago M Bandeiras
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica EAN, 2780 157 Oeiras, Portugal
    Biochim Biophys Acta 1787:37-45. 2009
    ..Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc(1) complexes of bacteria and mitochondria, however with distinct subunits and heme types...
  25. ncbi Control of oxidative phosphorylation by Complex I in rat liver mitochondria: implications for aging
    Barbara Ventura
    Dipartimento di Biochimica G. Moruzzi, , Via Irnerio 48, 40126, Bologna, Italy
    Biochim Biophys Acta 1553:249-60. 2002
    ..Our results point out that age-related alterations of the mitochondrial functions are also present in tissues considered less prone to accumulate mitochondrial DNA mutations...
  26. ncbi Reversible redox energy coupling in electron transfer chains
    Artur Osyczka
    The Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA
    Nature 427:607-12. 2004
    ....
  27. ncbi Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
    C Lange
    , Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt, Germany
    EMBO J 20:6591-600. 2001
    ..Site-directed mutagenesis of ligating residues confirmed the importance of the phosphatidylinositol- and cardiolipin-binding sites...
  28. ncbi Controlling the functionality of cytochrome c(1) redox potentials in the Rhodobacter capsulatus bc(1) complex through disulfide anchoring of a loop and a beta-branched amino acid near the heme-ligating methionine
    A Osyczka
    The Johnson Research Foundation, Department of Biochemistry and Biophysics, Plant Science Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 40:14547-56. 2001
    ..The disulfide anchored loop and betaXM motifs appear to be two independent but nonadditive strategies to control the integrity of the heme-binding pocket and raise cytochrome c midpoint potentials...
  29. ncbi Analysis of suppressor mutation reveals long distance interactions in the bc(1) complex of Saccharomyces cerevisiae
    G Brasseur
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, Marseilles, France
    Biochim Biophys Acta 1506:89-102. 2001
    ..Surprisingly, reversion at position 260 was located 13 A away from the original mutation and revealed a long distance interaction in the yeast bc(1) complex...
  30. ncbi Mitochondrial electron transport is a key determinant of life span in Caenorhabditis elegans
    J Feng
    Department of Biology, McGill University, 1205 Avenue Dr Penfield, H3A 1B1, , , Canada
    Dev Cell 1:633-44. 2001
    ..These findings indicate that both isp-1 and daf-2 mutations increase life span by lowering oxidative stress and result in the maximum life span increase that can be produced in this way...
  31. ncbi Oxygen sensing requires mitochondrial ROS but not oxidative phosphorylation
    Joslyn K Brunelle
    Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Cell Metab 1:409-14. 2005
    ..These findings provide genetic evidence that oxygen sensing is dependent on mitochondrial-generated reactive oxygen species (ROS) but independent of oxidative phosphorylation...
  32. ncbi Human disease-related mutations in cytochrome b studied in yeast
    Nicholas Fisher
    Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 279:12951-8. 2004
    ..Thus by using yeast as a model system, we identified the molecular basis of the respiratory defect caused by the disease mutations in cytochrome b...
  33. ncbi Modifications of protein environment of the [2Fe-2S] cluster of the bc1 complex: effects on the biophysical properties of the rieske iron-sulfur protein and on the kinetics of the complex
    Sangmoon Lhee
    Center for Biophysics and Computational Biology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 285:9233-48. 2010
    ..The proton-first-then-electron model proposed accounts naturally for the effects of mutation on the overall reaction...
  34. ncbi Discrimination between two possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc₁. Implications for the mechanism of superoxide production
    Marcin Sarewicz
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biochim Biophys Acta 1797:1820-7. 2010
    ..Isolation of this reaction sequence from multiplicity of possible reactions at Q(o) helps to better understand conditions under which complex III might contribute to ROS generation in vivo...
  35. ncbi Critical role of complex III in the early metabolic changes following myocardial infarction
    Lisa C Heather
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK
    Cardiovasc Res 85:127-36. 2010
    ..Therefore, we investigated cardiac mitochondrial metabolism shortly after infarction...
  36. ncbi Inhibitory effects of nitric oxide on invasion of human cancer cells
    Feng Wang
    Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA
    Cancer Lett 257:274-82. 2007
    ..Our findings suggest that NO inhibits invasion of cancer cells via both inhibition of HIF-1, and impairment of mitochondria...
  37. ncbi Intermonomer electron transfer in the bc1 complex dimer is controlled by the energized state and by impaired electron transfer between low and high potential hemes
    Vladimir P Shinkarev
    Department of Biochemistry, University of Illinois at Urbana Champaign, 156 Davenport Hall, 607 South Mathews Avenue, Urbana, IL 6l801, United States
    FEBS Lett 581:1535-41. 2007
    ..The effective rate of intermonomer transfer is about 2-orders of magnitude slower than the direct intermonomer electron transfer...
  38. ncbi Modeling the molecular basis of atovaquone resistance in parasites and pathogenic fungi
    Jacques J Kessl
    Center for Retrovirus Research, College of Pharmacy, Ohio State University, Columbus, OH 43210, USA
    Trends Parasitol 23:494-501. 2007
    ..To better understand the molecular basis of drug resistance, we have developed the yeast and bovine bc1 complexes as surrogates to model the molecular interaction of atovaquone with human and resistant pathogen enzymes...
  39. ncbi Cytochrome bc1-cy fusion complexes reveal the distance constraints for functional electron transfer between photosynthesis components
    Dong Woo Lee
    Department of Biology, Plant Science Institute
    J Biol Chem 283:13973-82. 2008
    ..These findings support the notion that membrane-bound Ps components form large, active structural complexes that are "hardwired" for cyclic ET...
  40. ncbi Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis
    Monika Czapla
    Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Protein Eng Des Sel 25:15-25. 2012
    ....
  41. ncbi Molecular basis of resistance to cytochrome bc1 inhibitors
    Nick Fisher
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, UK
    FEMS Yeast Res 8:183-92. 2008
    ..Four mutations that are of particular importance in medicine and in agriculture are briefly reviewed and described in more detail and the molecular basis of resistance and of evolution of the mutations is discussed succinctly...
  42. ncbi Proton-coupled electron transfer at the Q(o) site: what type of mechanism can account for the high activation barrier?
    A R Crofts
    Department of Biochemistry, University of Illinois, Urbana 61801, USA
    Biochim Biophys Acta 1459:456-66. 2000
    ....
  43. ncbi QO site deficiency can be compensated by extragenic mutations in the hinge region of the iron-sulfur protein in the bc1 complex of Saccharomyces cerevisiae
    Gael Brasseur
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 279:24203-11. 2004
    ....
  44. ncbi Mitochondrial complex III defects contribute to inefficient respiration and ATP synthesis in the myocardium of Trypanosoma cruzi-infected mice
    Jian Jun Wen
    Department of Microbiology and Immunology, The Center for Biodefense and Emerging Infectious Diseases, and The Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas 77555 1070, USA
    Antioxid Redox Signal 12:27-37. 2010
    ..Overall, we provide novel data on the mechanism(s) involved in cardiac bioenergetic inefficiency during T. cruzi infection...
  45. ncbi Movement of the iron-sulfur head domain of cytochrome bc(1) transiently opens the catalytic Q(o) site for reaction with oxygen
    Arkadiusz Borek
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biochemistry 47:12365-70. 2008
    ..We propose that in this way, cytochrome bc(1) under conditions of impeded electron flow employs the leak-proof short-circuits to minimize the unwanted single-electron reduction of oxygen...
  46. ncbi Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III
    Natalia V Dudkina
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Proc Natl Acad Sci U S A 102:3225-9. 2005
    ..We present a model for how complexes I and III are spatially organized within the I+III2 supercomplex...
  47. ncbi Magnetic interactions sense changes in distance between heme b(L) and the iron-sulfur cluster in cytochrome bc(1)
    Marcin Sarewicz
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biochemistry 48:5708-20. 2009
    ..The measured changes in the phase relaxation enhancement provide the first direct experimental description of changes in the strength of dipolar coupling between the FeS cluster and heme b(L)...
  48. ncbi The Q-cycle reviewed: How well does a monomeric mechanism of the bc(1) complex account for the function of a dimeric complex?
    Antony R Crofts
    Department of Biochemistry, University of Illinois at Urbana Champaign, IL 61801, USA
    Biochim Biophys Acta 1777:1001-19. 2008
    ..The data can also be interpreted as showing similar coulombic interaction across the dimer interface, and we discuss mechanistic implications...
  49. ncbi Aging decreases electron transport complex III activity in heart interfibrillar mitochondria by alteration of the cytochrome c binding site
    E J Lesnefsky
    Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Cell Cardiol 33:37-47. 2001
    ....
  50. ncbi Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1alpha during hypoxia: a mechanism of O2 sensing
    N S Chandel
    Department of Medicine, The University of Chicago, IL 60637, USA
    J Biol Chem 275:25130-8. 2000
    ..These findings reveal that mitochondria-derived ROS are both required and sufficient to initiate HIF-1alpha stabilization during hypoxia...
  51. ncbi A mutant mitochondrial respiratory chain assembly protein causes complex III deficiency in patients with tubulopathy, encephalopathy and liver failure
    P de Lonlay
    INSERM U393, Hopital Necker Enfants Malades, 149 rue de Sevres, 75015 Paris
    Nat Genet 29:57-60. 2001
    ..Complementation study in yeast confirmed the deleterious effect of these mutations. Mutation of BCS1L would seem to be a frequent cause of CIII deficiency, as one-third of our patients have BCS1L mutations...
  52. ncbi Bifurcated ubihydroquinone oxidation in the cytochrome bc1 complex by proton-gated charge transfer
    U Brandt
    Universitatsklinikum Frankfurt, Zentrum der Bioligischen Chemie, Frankfurt am Main, Germany
    FEBS Lett 387:1-6. 1996
    ..In this proton-gated charge-transfer mechanism the reaction is controlled by the deprotonation of the substrate ubihydroquinone...
  53. ncbi Deleterious epistatic interactions between electron transport system protein-coding loci in the copepod Tigriopus californicus
    Christopher S Willett
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    Genetics 173:1465-77. 2006
    ..These results suggest that the fitness consequences of the interactions of these three complex III-associated genes could influence reproductive isolation in this system...
  54. ncbi Mitochondrial respiratory chain-dependent generation of superoxide anion and its release into the intermembrane space
    D Han
    Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90089-9121
    Biochem J 353:411-6. 2001
    ....
  55. ncbi Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:13045-50. 2006
    ..A mechanism for the high fidelity of the bifurcated electron transfer is proposed...
  56. ncbi Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex
    S Iwata
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    Science 281:64-71. 1998
    ..These "core" subunits are related to the matrix processing peptidase, and the structure unveils how mitochondrial targeting presequences are recognized...
  57. ncbi Complex III releases superoxide to both sides of the inner mitochondrial membrane
    Florian L Muller
    Department of Cellular Biology, University of Texas Health Science Center, San Antonio, TX 78284 7762, USA
    J Biol Chem 279:49064-73. 2004
    ..This explains extramitochondrial release of superoxide but raises the question of how superoxide could reach the matrix. We discuss two models explaining this result...
  58. ncbi Fixing the Q cycle
    Artur Osyczka
    The Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Trends Biochem Sci 30:176-82. 2005
    ..Either the redox states of the quinone electron-transfer partners double-gate the semiquinone-intermediate stability, or semiquinone is avoided altogether in concerted double-electron transfer...
  59. ncbi A compilation of mutations located in the cytochrome b subunit of the bacterial and mitochondrial bc1 complex
    G Brasseur
    Department of Biology, University of Pennsylvania, Philadelphia 19104, USA
    Biochim Biophys Acta 1275:61-9. 1996
    ..In conjunction with the high resolution structure of the bc1 complex, this database is expected to serve as a useful reference point for the available data and help to focus and stimulate future experimental work in this field...
  60. ncbi Developmental regression and mitochondrial dysfunction in a child with autism
    Jon S Poling
    Department of Neurology and Neurosurgery, Johns Hopkins Hospital, Baltimore, MD, USA
    J Child Neurol 21:170-2. 2006
    ..These data suggest that further metabolic evaluation is indicated in autistic patients and that defects of oxidative phosphorylation might be prevalent...
  61. ncbi Genetics and the specificity of the aging process
    Siegfried Hekimi
    Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada
    Science 299:1351-4. 2003
    ..Thus, the aging process may be more specific than previously anticipated on evolutionary grounds...
  62. ncbi Architecture of active mammalian respiratory chain supercomplexes
    Eva Schäfer
    Department of Chemistry, Physical Biochemistry, Darmstadt University of Technology, Petersenstrasse 22, D 64287 Darmstadt, Germany
    J Biol Chem 281:15370-5. 2006
    ..Detailed knowledge of the architecture of the active supercomplexes is a prerequisite for a deeper understanding of energy conversion by mitochondria in mammals...
  63. ncbi Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment
    C Hunte
    Max Planck Institut fur Biophysik, Abt Molekulare Membranbiologie, Frankfurt, 60528, Germany
    Structure 8:669-84. 2000
    ..This integral membrane protein complex catalyzes electron transfer from ubiquinol to cytochrome c. It couples the electron transfer to the electrogenic translocation of protons across the membrane via a so-called Q cycle mechanism...
  64. ncbi Reaction intermediates of quinol oxidation in a photoactivatable system that mimics electron transfer in the cytochrome bc1 complex
    Jonathan L Cape
    Institute of Biological Chemistry, Washington State University, 289 Clark Hall, Pullman, Washington 99164-6314, USA
    J Am Chem Soc 127:4208-15. 2005
    ..The synthetic system is an unambiguous n=1 electron acceptor, and it is thus inferred that sequential oxidation of ubiquinol (by two sequential n=1 processes) is more rapid than a truly concerted (n=2) oxidation in the cyt bc1 complex...
  65. ncbi Breaking and re-forming the disulfide bond at the high-potential, respiratory-type Rieske [2Fe-2S] center of thermus thermophilus: characterization of the sulfhydryl state by protein-film voltammetry
    Yanbing Zu
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochemistry 41:14054-65. 2002
    ..This unique behavior is attributed to preorganization of the two thiolate groups upon uptake of one or more protons by the sulfhydryl pair...
  66. ncbi Interactions of quinone with the iron-sulfur protein of the bc(1) complex: is the mechanism spring-loaded?
    Antony R Crofts
    Department of Biochemistry, University of Illinois, Urbana 61801, USA
    Biochim Biophys Acta 1555:48-53. 2002
    ..We also discuss recent results from studies of the bypass reactions at the site, which lead to superoxide (SO) production under aerobic conditions, and provide additional information about intermediate states...
  67. ncbi Regulatory interactions in the dimeric cytochrome bc(1) complex: the advantages of being a twin
    Raul Covian
    Department of Biochemistry, Dartmouth Medical School, 7200 Vail, Hanover, New Hampshire 03755, USA
    Biochim Biophys Acta 1777:1079-91. 2008
    ....
  68. ncbi Plasmon waveguide resonance spectroscopic evidence for differential binding of oxidized and reduced Rhodobacter capsulatus cytochrome c2 to the cytochrome bc1 complex mediated by the conformation of the Rieske iron-sulfur protein
    S Devanathan
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 46:7138-45. 2007
    ....
  69. ncbi Domain conformational switch of the iron-sulfur protein in cytochrome bc1 complex is induced by the electron transfer from cytochrome bL to bH
    Chang An Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1777:1038-43. 2008
    ....
  70. ncbi Oxidative phosphorylation at the fin de siècle
    M Saraste
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Postfach 102209, D 69012, Heidelberg, Germany
    Science 283:1488-93. 1999
    ..This work, and new enzymological studies of ATP synthase's unusual catalytic mechanism, are reviewed here...
  71. ncbi Similar transition states mediate the Q-cycle and superoxide production by the cytochrome bc1 complex
    Isaac Forquer
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164 6340, USA
    J Biol Chem 281:38459-65. 2006
    ..Overall, the data support models where the rate-limiting step for both Q-cycle and superoxide production is essentially identical, consistent with model 1 but requiring modifications to models 2 and 3...
  72. ncbi Assembly of the oxidative phosphorylation system in humans: what we have learned by studying its defects
    Erika Fernandez-Vizarra
    Department of Molecular Neurogenetics, IRCCS Foundation Neurological Institute C Besta, 20126 Milan, Italy
    Biochim Biophys Acta 1793:200-11. 2009
    ..We review here current knowledge of the biogenesis of OXPHOS complexes based on investigation of the corresponding disorders...
  73. ncbi Asymmetric and redox-specific binding of quinone and quinol at center N of the dimeric yeast cytochrome bc1 complex. Consequences for semiquinone stabilization
    Raul Covian
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 282:24198-208. 2007
    ..This model allows maximum SQ.bH(3+) formation without inhibition of Q binding by QH2...
  74. ncbi The loneliness of the electrons in the bc1 complex
    Stéphane Ransac
    Universite de Bordeaux 2, 146 rue Leo Saignat, F 33076, Bordeaux Cedex, France
    Biochim Biophys Acta 1777:1053-9. 2008
    ..Thus, in our paper, the Mitchell Q-cycle no longer appears as an a priori hypothesis but arises out of the bc(1) complex structure and of the kinetic laws of redox reactions...
  75. ncbi Quinone and non-quinone redox couples in Complex III
    Haibo Zhang
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Bioenerg Biomembr 40:493-9. 2008
    ..The redox properties of the most obscure component, Qo, is finally beginning to be resolved...
  76. ncbi Multiple Q-cycle bypass reactions at the Qo site of the cytochrome bc1 complex
    Florian Muller
    Institute of Biological Chemistry, 289 Clark Hall, Washington State University, Pullman, WA 99164-6340, USA
    Biochemistry 41:7866-74. 2002
    ..Given that myxothiazol blocks cyt b reduction whereas antimycin A promotes it, we propose that this second bypass occurs by reduction of the Q(o) site semiquinone by prereduced cyt b(L)...
  77. ncbi Atomic-level structural and functional model of a bacterial photosynthetic membrane vesicle
    Melih K Sener
    Beckman Institute and Department of Physics, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 104:15723-8. 2007
    ....
  78. ncbi GRACILE syndrome, a lethal metabolic disorder with iron overload, is caused by a point mutation in BCS1L
    Ilona Visapää
    Department of Human Genetics, University of California Los Angeles School of Medicine, USA
    Am J Hum Genet 71:863-76. 2002
    ....
  79. ncbi Reconstitution of mitochondrial processing peptidase from the core proteins (subunits I and II) of bovine heart mitochondrial cytochrome bc(1) complex
    K Deng
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 276:6499-505. 2001
    ..The aromatic group, rather than the hydroxyl group, at Tyr(57) of core I is essential for reconstitutive activity...
  80. ncbi Respiratory complex III is required to maintain complex I in mammalian mitochondria
    Rebeca Acín-Pérez
    Departamento de Bioquimica y Biologia Molecular y Celular, Universidad de Zaragoza, Miguel Servet, 177, Zaragoza 50013, Spain
    Mol Cell 13:805-15. 2004
    ..Conversely, complex III stability was not influenced by the absence of complex I. This structural dependence among complexes I and III was confirmed in a muscle biopsy of a patient harboring a nonsense cytochrome b mutation...
  81. ncbi Retinal ganglion cell axotomy induces an increase in intracellular superoxide anion
    Christopher J Lieven
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison, Wisconsin 53792, USA
    Invest Ophthalmol Vis Sci 47:1477-85. 2006
    ..CONCLUSIONS: These findings suggest that superoxide generated in the mitochondrial electron transport chain could be a parallel system to neurotrophic deprivation for signaling cell death after axonal injury...
  82. ncbi Certain metal ions are inhibitors of cytochrome b6f complex 'Rieske' iron-sulfur protein domain movements
    Arthur G Roberts
    Institute of Biological Chemistry, Washington State University, 289 Clark Hall, Pullman, Washington 99164-6340, USA
    Biochemistry 41:4070-9. 2002
    ..These results support the role of ISP domain movements in Q(o) site catalysis...
  83. ncbi Impact of mitochondria and NADPH oxidases on acute and sustained hypoxic pulmonary vasoconstriction
    Norbert Weissmann
    University Giessen Lung Centre UGLC, Medical Clinic II V, Justus Liebig University Giessen, Klinikstrasse 36, 35392 Giessen, Germany
    Am J Respir Cell Mol Biol 34:505-13. 2006
    ....
  84. ncbi Characterization of superoxide-producing sites in isolated brain mitochondria
    Alexei P Kudin
    Department of Epileptology, University Bonn Medical Center, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany
    J Biol Chem 279:4127-35. 2004
    ....
  85. ncbi Electron tunneling chains of mitochondria
    Christopher C Moser
    The Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, 1005, Stellar Chance Laboratories, 422, Curie Boulevard, Philadelphia, PA 19104 6059, USA
    Biochim Biophys Acta 1757:1096-109. 2006
    ..Tunneling simulations of the respiratory complexes provide clear illustrations of this simple engineering...
  86. ncbi Structural basis of multifunctional bovine mitochondrial cytochrome bc1 complex
    C A Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078 3035, USA
    J Bioenerg Biomembr 31:191-9. 1999
    ..The superoxide-generation site of the cytochrome bc1 complex is located at reduced bL and Q*-. The reaction is membrane potential-, and cytochrome c-dependent...
  87. ncbi Subcomplexes of human ATP synthase mark mitochondrial biosynthesis disorders
    Rosalba Carrozzo
    Unit of Molecular Medicine, , Rome, Italy
    Ann Neurol 59:265-75. 2006
    ....
  88. ncbi Binding dynamics at the quinone reduction (Qi) site influence the equilibrium interactions of the iron sulfur protein and hydroquinone oxidation (Qo) site of the cytochrome bc1 complex
    Jason W Cooley
    Department of Biology, Plant Science Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 44:10520-32. 2005
    ..Implications of these findings in respect to the Q(o)-Q(i) sites communications and to multiple turnovers of the cyt bc(1) are discussed...
  89. ncbi Understanding the cytochrome bc complexes by what they don't do. The Q-cycle at 30
    Jonathan L Cape
    Institute of Biological Chemistry, Washington State University, 289 Clark Hall, Pullman, WA 99164-6314, USA
    Trends Plant Sci 11:46-55. 2006
    ..Current models to explain the avoidance of side reactions involve unprecedented or unusual enzyme mechanisms, the testing of which will involve new theoretical and experimental approaches...
  90. ncbi Structure and function of cytochrome bc complexes
    E A Berry
    Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Annu Rev Biochem 69:1005-75. 2000
    ....
  91. ncbi Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc1 complex
    Gregory Engstrom
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Biochemistry 42:2816-24. 2003
    ..The interaction of yeast Ru-39-Cc with yeast cytochrome bc1 is stronger than that of horse Ru-39-Cc with bovine cytochrome bc1, suggesting that nonpolar interactions are stronger in the yeast system...
  92. ncbi Protonmotive pathways and mechanisms in the cytochrome bc1 complex
    Carola Hunte
    Department Molecular Membrane Biology, Max Planck Institute Biophysics, D 60439 Frankfurt am Main, Germany
    FEBS Lett 545:39-46. 2003
    ..In this review we discuss pathways for proton conduction linked to ubiquinone redox reactions with particular reference to recently determined structures of the yeast bc(1) complex...
  93. ncbi Membrane potential greatly enhances superoxide generation by the cytochrome bc1 complex reconstituted into phospholipid vesicles
    Hagai Rottenberg
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 284:19203-10. 2009
    ....
  94. ncbi Across membrane communication between the Q(o) and Q(i) active sites of cytochrome bc(1)
    Jason W Cooley
    Department of Biology, Plant Science Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 48:1888-99. 2009
    ....
  95. ncbi Inhibitory analogs of ubiquinol act anti-cooperatively on the Yeast cytochrome bc1 complex. Evidence for an alternating, half-of-the-sites mechanism of ubiquinol oxidation
    Emma Berta Gutierrez-Cirlos
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 277:1195-202. 2002
    ..Because these inhibitors bind to the ubiquinol oxidation site in the bc(1) complex, we propose that the yeast cytochrome bc(1) complex oxidizes ubiquinol by an alternating, half-of-the-sites mechanism...
  96. ncbi Evolution of interacting proteins in the mitochondrial electron transport system in a marine copepod
    Christopher S Willett
    Department of Biology, University of North Carolina, Chapel Hill, USA
    Mol Biol Evol 21:443-53. 2004
    ..Finally, a comparison of nuclear-encoded and mitochondrial-encoded genes from T. californicus suggests that substitution rates in the mitochondrial-encoded genes are dramatically increased relative to nuclear genes...
  97. ncbi Substrate redox potential controls superoxide production kinetics in the cytochrome bc complex
    Jonathan L Cape
    Institute of Biological Chemistry, Washington State University, 289 Clark Hall, Pullman, Washington 99164 6314, USA
    Biochemistry 48:10716-23. 2009
    ....
  98. ncbi The quinone chemistry of bc complexes
    Peter R Rich
    The Glynn Laboratory of Bioenergetics, Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    Biochim Biophys Acta 1658:165-71. 2004
    ....
  99. ncbi Mitochondrial superoxide radical formation is controlled by electron bifurcation to the high and low potential pathways
    Katrin Staniek
    Institute of Pharmacology and Toxicology, Veterinary University Vienna, Austria
    Free Radic Res 36:381-7. 2002
    ..The regulator, which controls leakage of electrons to oxygen, appears to be the electron-branching activity of the cytochrome bc1 complex...
  100. ncbi Sources for superoxide release: lessons from blockade of electron transport, NADPH oxidase, and anion channels in diaphragm
    Li Zuo
    The Ohio State University Medical Centre, Department of Internal Medicine, Dorothy M. Davis Heart and Lung Research Institute, Division of Pulmonary and Critical Care Medicine, Columbus, OH 43210, USA
    Antioxid Redox Signal 5:667-75. 2003
    ..Although the molecular source for extracellular O2*- remains elusive, it is clearly sensitive to temperature and conditions of "chemical hypoxia" induced by partial or complete mitochondrial inhibition...
  101. ncbi Ischemic preconditioning preserves mitochondrial function after global cerebral ischemia in rat hippocampus
    K R Dave
    Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, Miami, Florida, USA
    J Cereb Blood Flow Metab 21:1401-10. 2001
    ..These data suggest that IPC protects the integrity of mitochondrial oxidative phosphorylation after cerebral ischemia...

Research Grants88

  1. HYDROPHOBIC ASSOCIATIONS OF MITOCHONDRIAL PROTEINS
    NEAL ROBINSON; Fiscal Year: 1993
    ..several different membrane proteins, structural features that are common among these cardiolipin dependent proteins will be determined, and the molecular basis of cardiolipin participation in mitochondrial function will be established..
  2. BIOENERGETICS OF THE CYTOCHROME BC1 COMPLEX
    DIANA BEATTIE; Fiscal Year: 2001
    ..performed to determine whether charged amino acid residues ar required for efficient assembly of the ISP into the bc1 complex and for enzymatic activity. ..
  3. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    Neal C Robinson; Fiscal Year: 2010
    ..Successful completion of this project will determine the absolute requirement for cardiolipin and help us understand its pathophysiological importance. ..
  4. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2009
    ....
  5. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2010
    ....
  6. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    Neal C Robinson; Fiscal Year: 2011
    ..Successful completion of this project will determine the absolute requirement for cardiolipin and help us understand its pathophysiological importance. ..
  7. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    NEAL ROBINSON; Fiscal Year: 2009
    ..Successful completion of this project will determine the absolute requirement for cardiolipin and help us understand its pathophysiological importance. ..
  8. MOLECULAR GENETICS OF THE CYTOCHROME BC1 C COMPLEX
    Fevzi Daldal; Fiscal Year: 1993
    ..This work also contributes to a better recognition of the interactions between the subunits of membrane proteins and their prosthetic groups during their biogenesis and assembly...
  9. Charge Transfer Reactions of the Cytochrome bc1 Complex
    Colin A Wraight; Fiscal Year: 2010
    ..Several chronic and congenital diseases (myopathies) also arise from malfunctioning bc1 complex. Finally, this activity is a target for new therapeutic agents, especially for fungi and parasitic protists. ..
  10. Charge Transfer Reactions of the Cytochrome bc1 Complex
    COLIN WRAIGHT; Fiscal Year: 2007
    ..Several chronic and congenital diseases (myopathies) also arise from malfunctioning bc1 complex. Finally, this activity is a target for new therapeutic agents, especially for fungi and parasitic protists. ..
  11. Charge Transfer Reactions of the Cytochrome bc1 Complex
    COLIN WRAIGHT; Fiscal Year: 2009
    ..Several chronic and congenital diseases (myopathies) also arise from malfunctioning bc1 complex. Finally, this activity is a target for new therapeutic agents, especially for fungi and parasitic protists. ..
  12. ELECTRON AND PROTON TRANSLOCATION IN BIOLOGICAL ENERGY
    P Dutton; Fiscal Year: 2006
    ..As advances are made we will extend techniques to cytochrome oxidase and other oxidoreductases. ..
  13. ELECTRON-TRANSFER PROTEINS
    FRANCIS S contact MILLETT; Fiscal Year: 2010
    ..In the proposed studies, new ruthenium photoexcitation methods will be developed to study electron transfer reactions important to human health. ..
  14. Complex III Qo site energy transduction
    PETER LESLIE DUTTON; Fiscal Year: 2010
    ....
  15. Complex III Qo site energy transduction
    P Dutton; Fiscal Year: 2009
    ....
  16. STRUCTURE/FUNCTION OF THE CYTOCHROME B6F COMPLEX
    WILLIAM CRAMER; Fiscal Year: 2003
    ..Ruthenated cytf will also be used to investigate intraprotein protonation- deprotonation at specific carboxylates, associated with coupled electron and proton transfer. ..
  17. MOLECULAR GENETICS OF THE CYTOCHROME BC1 COMPLEX
    M FEVZI DALDAL; Fiscal Year: 2004
    ..abstract_text> ..
  18. MECHANISM OF RESPIRATION AND ENERGY TRANSDUCTION
    Bernard Trumpower; Fiscal Year: 2009
    ..abstract_text> ..
  19. ELECTRON TRANSFER PROTEINS
    Francis Millett; Fiscal Year: 2002
    ..A major goal will be to determine the pathway and kinetics of electron transfer from the Rieske iron-sulfur center to cytochrome c1 and to cytochrome c. ..
  20. PHOSPHOLIPIDS AND MITOCHONDRIAL FUNCTION
    William Dowhan; Fiscal Year: 2006
    ..Defining the role these lipids play in normal mitochondrial function will shed light on the molecular basis for cellular dysfunction in diseases where these lipids are reduced. ..
  21. CHARGE TRANSFER REACTIONS OF THE BE COMPLEX
    COLIN WRAIGHT; Fiscal Year: 1999
    ..v) Deconvolution of spectral, kinetic and thermodynamic parameters. (vi) Use of selected mutants to limit or eliminate specific reactions. ..
  22. MOLECULAR GENETICS OF THE CYTOCHROME BC1 COMPLEX
    Fevzi Daldal; Fiscal Year: 1999
    ....
  23. FUNCTION OF CARDIOLIPIN IN CYTOCHROME C OXIDASE AND BC1
    NEAL ROBINSON; Fiscal Year: 1999
    ..Results obtained with these two similar, but different complexes will allow us to determine whether CL has unique or universal roles in coupled electron transport. ..
  24. 3-D CRYSTAL STRUCTURE:VERTEBRATE MITOCHONDRIA COMPLEX II
    EDWARD BERRY; Fiscal Year: 2002
    ..C) Build an atomic model of the protein into the electron density map and refine it against the diffraction data, for submission to the protein data bank. ..
  25. Structure of reaction intermediates in the bc1 complex
    SERGEI DIKANOV; Fiscal Year: 2007
    ..The central role of bc1 complex plays out in many medical scenarios in which defects lead to pathology, among them cellular death through ROS-mediated damage, mitochondrial myopathies, and apoptosis, and in drug targeting. ..
  26. Structure of reaction intermediates in the bc1 complex
    SERGEI DIKANOV; Fiscal Year: 2009
    ..The central role of bc1 complex plays out in many medical scenarios in which defects lead to pathology, among them cellular death through ROS-mediated damage, mitochondrial myopathies, and apoptosis, and in drug targeting. ..
  27. Structure of reaction intermediates in the bc1 complex
    Sergei A Dikanov; Fiscal Year: 2010
    ..The central role of bc1 complex plays out in many medical scenarios in which defects lead to pathology, among them cellular death through ROS-mediated damage, mitochondrial myopathies, and apoptosis, and in drug targeting. ..
  28. Charge Transfer Reactions of the Cytochrome bc1 Complex
    COLIN WRAIGHT; Fiscal Year: 2006
    ..v) Deconvolution of spectral, kinetic and thermodynamicparameters, (vi) Use of selected mutants in a histidine-tagged environment to limit or eliminate specific reactions or states of the bC[complex. ..
  29. MAPPING OF ELECTRON TUNNELING PATHWAYS IN PROTEINS
    David Beratan; Fiscal Year: 2006
    ..abstract_text> ..
  30. ELECTRON-TRANSFER PROTEINS
    Francis Millett; Fiscal Year: 2006
    ..Major goals will be to determine the pathway and kinetics of electron transfer from cytochrome c through CuA and heme a to the heme a3--CuB binuclear center, as well as coupled proton uptake and release. ..