electron transport complex iii

Summary

Summary: A multisubunit enzyme complex that contains CYTOCHROME B GROUP; CYTOCHROME C1; and iron-sulfur centers. It catalyzes the oxidation of ubiquinol to UBIQUINONE, and transfers the electrons to CYTOCHROME C. In MITOCHONDRIA the redox reaction is coupled to the transport of PROTONS across the inner mitochondrial membrane.

Top Publications

  1. ncbi Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing
    Robert D Guzy
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    Cell Metab 1:401-8. 2005
  2. ncbi Production of reactive oxygen species by mitochondria: central role of complex III
    Qun Chen
    Department of Medicine, Division of Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:36027-31. 2003
  3. ncbi Respiratory active mitochondrial supercomplexes
    Rebeca Acín-Pérez
    Departamento de Bioquimica, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
    Mol Cell 32:529-39. 2008
  4. ncbi Electron transfer by domain movement in cytochrome bc1
    Z Zhang
    E O Lawrence Berkeley National Laboratory, University of California, 94720, USA
    Nature 392:677-84. 1998
  5. ncbi Mutations in TTC19 cause mitochondrial complex III deficiency and neurological impairment in humans and flies
    Daniele Ghezzi
    Unit of Molecular Neurogenetics, The Foundation Carlo Besta Institute of Neurology, Milan, Italy
    Nat Genet 43:259-63. 2011
  6. pmc Respiratory complex III is required to maintain complex I in mammalian mitochondria
    Rebeca Acín-Pérez
    Departamento de Bioquimica y Biologia Molecular y Celular, Universidad de Zaragoza, Miguel Servet, 177, Zaragoza 50013, Spain
    Mol Cell 13:805-15. 2004
  7. ncbi The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase
    Julie Bachmann
    Institut fur Biochemie, Johann Wolfgang Goethe Universitat, D 60438 Frankfurt, Germany
    FEBS J 273:4817-30. 2006
  8. ncbi Mitochondrial electron transport is a key determinant of life span in Caenorhabditis elegans
    J Feng
    Department of Biology, McGill University, 1205 Avenue Dr Penfield, H3A 1B1, , , Canada
    Dev Cell 1:633-44. 2001
  9. ncbi Inhibitor-complexed structures of the cytochrome bc1 from the photosynthetic bacterium Rhodobacter sphaeroides
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:2846-57. 2008
  10. ncbi Two modes of mitochondrial dysfunction lead independently to lifespan extension in Caenorhabditis elegans
    Wen Yang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Aging Cell 9:433-47. 2010

Detail Information

Publications262 found, 100 shown here

  1. ncbi Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing
    Robert D Guzy
    Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA
    Cell Metab 1:401-8. 2005
    ..These results demonstrate that mitochondria function as O(2) sensors and signal hypoxic HIF-1 alpha and HIF-2 alpha stabilization by releasing ROS to the cytosol...
  2. ncbi Production of reactive oxygen species by mitochondria: central role of complex III
    Qun Chen
    Department of Medicine, Division of Cardiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:36027-31. 2003
    ..In mitochondria, complex III is the principal site for ROS generation during the oxidation of complex I substrates, and rotenone protects by limiting electron flow into complex III...
  3. ncbi Respiratory active mitochondrial supercomplexes
    Rebeca Acín-Pérez
    Departamento de Bioquimica, Universidad de Zaragoza, Miguel Servet, 177, 50013 Zaragoza, Spain
    Mol Cell 32:529-39. 2008
    ..Therefore, we have demonstrated the existence of a functional respirasome and propose a structural organization model that accommodates these findings...
  4. ncbi Electron transfer by domain movement in cytochrome bc1
    Z Zhang
    E O Lawrence Berkeley National Laboratory, University of California, 94720, USA
    Nature 392:677-84. 1998
    ..Such a mechanism has not previously been observed in redox protein complexes...
  5. ncbi Mutations in TTC19 cause mitochondrial complex III deficiency and neurological impairment in humans and flies
    Daniele Ghezzi
    Unit of Molecular Neurogenetics, The Foundation Carlo Besta Institute of Neurology, Milan, Italy
    Nat Genet 43:259-63. 2011
    ..TTC19 is a putative cIII assembly factor whose disruption is associated with severe neurological abnormalities in humans and flies...
  6. pmc Respiratory complex III is required to maintain complex I in mammalian mitochondria
    Rebeca Acín-Pérez
    Departamento de Bioquimica y Biologia Molecular y Celular, Universidad de Zaragoza, Miguel Servet, 177, Zaragoza 50013, Spain
    Mol Cell 13:805-15. 2004
    ..Conversely, complex III stability was not influenced by the absence of complex I. This structural dependence among complexes I and III was confirmed in a muscle biopsy of a patient harboring a nonsense cytochrome b mutation...
  7. ncbi The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase
    Julie Bachmann
    Institut fur Biochemie, Johann Wolfgang Goethe Universitat, D 60438 Frankfurt, Germany
    FEBS J 273:4817-30. 2006
    ..Mutation of an essential cofactor binding residue (C152S) decreases the ISP membrane levels, possibly indicating that cofactor insertion is a prerequisite for efficient translocation along the Tat pathway...
  8. ncbi Mitochondrial electron transport is a key determinant of life span in Caenorhabditis elegans
    J Feng
    Department of Biology, McGill University, 1205 Avenue Dr Penfield, H3A 1B1, , , Canada
    Dev Cell 1:633-44. 2001
    ..These findings indicate that both isp-1 and daf-2 mutations increase life span by lowering oxidative stress and result in the maximum life span increase that can be produced in this way...
  9. ncbi Inhibitor-complexed structures of the cytochrome bc1 from the photosynthetic bacterium Rhodobacter sphaeroides
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:2846-57. 2008
    ..Functional implications for the bc(1) complex are derived from analyses of 10 independent molecules in various crystal forms and from comparisons with mitochondrial complexes...
  10. ncbi Two modes of mitochondrial dysfunction lead independently to lifespan extension in Caenorhabditis elegans
    Wen Yang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Aging Cell 9:433-47. 2010
    ..Our results demonstrate that distinct and separable aspects of mitochondrial biology affect lifespan independently...
  11. ncbi Structure of the yeast cytochrome bc1 complex with a hydroxyquinone anion Qo site inhibitor bound
    Hildur Palsdottir
    Abt Molekulare Membranbiologie, Max Planck Institut fur Biophysik, Marie Curie Strasse 15, D 60439 Frankfurt, Germany
    J Biol Chem 278:31303-11. 2003
    ..Binding of the alkyl-6-hydroxy-4,7-dioxobenzothiazole is discussed as resembling an intermediate step of ubiquinol oxidation, supporting a single occupancy model at the Qo site...
  12. ncbi Generation of reactive oxygen species by the mitochondrial electron transport chain
    Yuanbin Liu
    Cellular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 9062, USA
    J Neurochem 80:780-7. 2002
    ..These new insights clarify an elusive target for intervening mitochondrial ROS-related processes or diseases...
  13. ncbi The Rieske Fe/S protein of the cytochrome b6/f complex in chloroplasts: missing link in the evolution of protein transport pathways in chloroplasts?
    S Molik
    Institut für Pflanzenphysiologie, Martin Luther Universitat Halle Wittenberg, Weinbergweg 10, D 06120 Halle Saale, Germany
    J Biol Chem 276:42761-6. 2001
    ..These untypical features suggest that the Rieske protein represents an intermediate or early state in the evolution of the thylakoidal protein transport pathways...
  14. pmc Mitochondrial respiratory chain-dependent generation of superoxide anion and its release into the intermembrane space
    D Han
    Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90089-9121
    Biochem J 353:411-6. 2001
    ....
  15. ncbi Conformationally linked interaction in the cytochrome bc(1) complex between inhibitors of the Q(o) site and the Rieske iron-sulfur protein
    Edward A Berry
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA
    Biochim Biophys Acta 1807:1349-63. 2011
    ..This article is part of a Special Issue entitled: Allosteric cooperativity in respiratory proteins...
  16. ncbi Physiological diversity of mitochondrial oxidative phosphorylation
    G Benard
    INSERM U688, Physiopathologie Mitochondriale, Universite Victor Segalen Bordeaux 2, Bordeaux, France
    Am J Physiol Cell Physiol 291:C1172-82. 2006
    ..To conclude, we submitted our data to a principal component analysis that revealed three groups of tissues: muscle and heart, brain, and liver and kidney...
  17. ncbi Energy thresholds in brain mitochondria. Potential involvement in neurodegeneration
    G P Davey
    Department of Neurochemistry, Institute of Neurology, Queen Square, London WC1N 3BG, United Kingdom
    J Biol Chem 273:12753-7. 1998
    ..The implications of these findings are discussed with respect to neurodegenerative disorders and energy metabolism in the synapse...
  18. ncbi Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase
    B Kauppi
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Structure 6:571-86. 1998
    ..The present study was conducted to provide essential structural information necessary for elucidating the mechanism of action of NDO...
  19. ncbi Activity of complex III of the mitochondrial electron transport chain is essential for early heart muscle cell differentiation
    Dimitry Spitkovsky
    Institute of Vegetative Physiology, Center of Physiology and Pathophysiology, The University of Köln, Germany
    FASEB J 18:1300-2. 2004
    ..We postulate that mitochondrial complex III activity is necessary for these Ca++ oscillations, which in turn are a prerequisite for cardiomyocyte differentiation...
  20. pmc Cells lacking Rieske iron-sulfur protein have a reactive oxygen species-associated decrease in respiratory complexes I and IV
    Francisca Diaz
    Department of Neurology, University of Miami, Miller School of Medicine, Miami, Florida, USA
    Mol Cell Biol 32:415-29. 2012
    ..Our data suggest that the stability of CI, CIV, and supercomplexes is regulated by ROS in the context of defective oxidative phosphorylation...
  21. ncbi Cytochrome b mutations that modify the ubiquinol-binding pocket of the cytochrome bc1 complex and confer anti-malarial drug resistance in Saccharomyces cerevisiae
    Jacques J Kessl
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755
    J Biol Chem 280:17142-8. 2005
    ..By modeling the variations in cytochrome b structure and atovaquone binding with the mutated bc1 complexes, we obtained the first quantitative explanation for the molecular basis of atovaquone resistance in malaria parasites...
  22. ncbi Proteomic analysis of mitochondria-to-nucleus retrograde response in human cancer
    Mariola Kulawiec
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York, USA
    Cancer Biol Ther 5:967-75. 2006
    ..Our study opens an avenue for identification of retrograde proteins as potential tumor suppressors or oncogenes involved in carcinogenesis...
  23. ncbi Threshold effect and tissue specificity. Implication for mitochondrial cytopathies
    R Rossignol
    INSERM EMI 9929, , , F 33076 Bordeaux cedex, France
    J Biol Chem 274:33426-32. 1999
    ..We propose a classification of tissues according to their type of OXPHOS response to a complex deficiency and therefore to their threshold values...
  24. ncbi Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    J Mol Biol 341:281-302. 2004
    ..Class P contains two subgroups, Pm and Pf, that are distinct by their ability to induce mobile or fixed conformation of iron-sulfur protein...
  25. ncbi Rates of behavior and aging specified by mitochondrial function during development
    Andrew Dillin
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0448, USA
    Science 298:2398-401. 2002
    ..Thus, the developing animal appears to contain a regulatory system that monitors mitochondrial activity early in life and, in response, establishes rates of respiration, behavior, and aging that persist during adulthood...
  26. ncbi Role of phospholipids in respiratory cytochrome bc(1) complex catalysis and supercomplex formation
    Tina Wenz
    Max Planck Institute of Biophysics, Department of Molecular Membrane Biology, D 60438 Frankfurt, Germany
    Biochim Biophys Acta 1787:609-16. 2009
    ..Overall, the study supports the idea, that enzyme-bound phospholipids can play an important role in the regulation of protein function and protein-protein interaction...
  27. ncbi Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1alpha during hypoxia: a mechanism of O2 sensing
    N S Chandel
    Department of Medicine, The University of Chicago, IL 60637, USA
    J Biol Chem 275:25130-8. 2000
    ..These findings reveal that mitochondria-derived ROS are both required and sufficient to initiate HIF-1alpha stabilization during hypoxia...
  28. ncbi A novel nonsense mutation (Q352X) in the mitochondrial cytochrome b gene associated with a combined deficiency of complexes I and III
    Eleonora Lamantea
    Division of Biochemistry and Genetics, National Neurological Institute C Besta Via Celoria, 11 20133 Milan, Italy
    Neuromuscul Disord 12:49-52. 2002
    ..Clinical presentation and laboratory findings strongly support the hypothesis that this mutation is the primary cause of the disease in our patient...
  29. ncbi Mitochondrial complex III deficiency caused by a homozygous UQCRC2 mutation presenting with neonatal-onset recurrent metabolic decompensation
    Noriko Miyake
    Department of Human Genetics, Yokohama City University Graduate School of Medicine, Yokohama, Japan
    Hum Mutat 34:446-52. 2013
    ..This is the first described human disease caused by a core protein abnormality in mitochondrial CIII...
  30. ncbi Acute mitochondrial actions of glitazones on the liver: a crucial parameter for their antidiabetic properties
    María Nieves Sanz
    Department of Physiology and Pharmacology, University of Salamanca, Salamanca, Spain
    Cell Physiol Biochem 28:899-910. 2011
    ..However, TZDs action does not exclude independent PPARγ-activation effects. Remarkably, direct mitochondrial action of these agents has not been fully studied yet...
  31. ncbi Roles of the disulfide bond and adjacent residues in determining the reduction potentials and stabilities of respiratory-type Rieske clusters
    Ellen J Leggate
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge, UK
    Biochemistry 44:7048-58. 2005
    ..The sum of the effects of all the mutated residues accounts for most of the differences between high- and low-potential Rieske proteins...
  32. ncbi Fertility-related proteomic profiling bull spermatozoa separated by percoll
    Yoo Jin Park
    Department of Animal Science and Technology, School of Bioresource and Bioscience, Chung Ang University, Anseong, Gyeonggi do 456 756, Korea
    J Proteome Res 11:4162-8. 2012
    ..Therefore, these results suggest that concurrent comparisons between protein expression and other fertility assays may represent a good in vitro assay to determine sperm fertility...
  33. ncbi Mitochondrial respiratory chain super-complex I-III in physiology and pathology
    Giorgio Lenaz
    Dipartimento di Biochimica, Universita di Bologna, Via Irnerio 48, 40126 Bologna, Italy
    Biochim Biophys Acta 1797:633-40. 2010
    ..There is increasing evidence that disruption of the super-complex organization leads to functional derangements responsible for pathological changes, as we have found in K-ras-transformed fibroblasts...
  34. ncbi The GRACILE mutation introduced into Bcs1l causes postnatal complex III deficiency: a viable mouse model for mitochondrial hepatopathy
    Per Leveen
    Department of Pediatrics, Clinical Sciences, Lund University, Lund, Sweden
    Hepatology 53:437-47. 2011
    ..Complex I function, suggested to be dependent on a functional complex III, was, however, unaffected...
  35. pmc Enzymatic activities of isolated cytochrome bc₁-like complexes containing fused cytochrome b subunits with asymmetrically inactivated segments of electron transfer chains
    Monika Czapla
    Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biochemistry 51:829-35. 2012
    ....
  36. ncbi Bupivacaine uncouples the mitochondrial oxidative phosphorylation, inhibits respiratory chain complexes I and III and enhances ROS production: results of a study on cell cultures
    Olga Cela
    Department of Biomedical Sciences, University of Foggia, Foggia, Italy
    Mitochondrion 10:487-96. 2010
    ....
  37. pmc Hypoxia-inducible factor-1 activation in nonhypoxic conditions: the essential role of mitochondrial-derived reactive oxygen species
    David A Patten
    Centre de recherche du CHUQ, L Hôtel Dieu de Québec, Department of Molecular Biology, Medical Biochemistry and Pathology, Universite Laval, Quebec, QC, G1R 2J6, Canada
    Mol Biol Cell 21:3247-57. 2010
    ..However, our work points to mitochondrial-generated ROS as essential intermediates for HIF-1 activation in nonhypoxic conditions...
  38. pmc Late-stage maturation of the Rieske Fe/S protein: Mzm1 stabilizes Rip1 but does not facilitate its translocation by the AAA ATPase Bcs1
    Tie Zhong Cui
    University of Utah Health Sciences Center, Department of Medicine, Salt Lake City, Utah, USA
    Mol Cell Biol 32:4400-9. 2012
    ..However, the Rip1 variants were not stably associated with the supercomplex. The induced supercomplex stabilization by the Rip1 N terminus was independent of Mzm1...
  39. pmc Intermonomer electron transfer in the bc1 complex dimer is controlled by the energized state and by impaired electron transfer between low and high potential hemes
    Vladimir P Shinkarev
    Department of Biochemistry, University of Illinois at Urbana Champaign, 156 Davenport Hall, 607 South Mathews Avenue, Urbana, IL 6l801, United States
    FEBS Lett 581:1535-41. 2007
    ..The effective rate of intermonomer transfer is about 2-orders of magnitude slower than the direct intermonomer electron transfer...
  40. pmc Developmental regression and mitochondrial dysfunction in a child with autism
    Jon S Poling
    Department of Neurology and Neurosurgery, Johns Hopkins Hospital, Baltimore, MD, USA
    J Child Neurol 21:170-2. 2006
    ..These data suggest that further metabolic evaluation is indicated in autistic patients and that defects of oxidative phosphorylation might be prevalent...
  41. ncbi Missense mutations in the BCS1L gene as a cause of the Björnstad syndrome
    J Travis Hinson
    Harvard Medical School, Boston, MA 02115, USA
    N Engl J Med 356:809-19. 2007
    ..The Björnstad syndrome, an autosomal recessive disorder associated with sensorineural hearing loss and pili torti, is caused by mutation of a previously unidentified gene on chromosome 2q34-36...
  42. ncbi Human disease-related mutations in cytochrome b studied in yeast
    Nicholas Fisher
    Wolfson Institute for Biomedical Research, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 279:12951-8. 2004
    ..Thus by using yeast as a model system, we identified the molecular basis of the respiratory defect caused by the disease mutations in cytochrome b...
  43. ncbi Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b
    Vincenzo Zara
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita di Lecce, Italy
    Eur J Biochem 271:1209-18. 2004
    ..Likewise, evidence of interactions between subunit 6, subunit 9 and cytochrome c1 suggests that a subcomplex between these two supernumerary subunits and the cytochrome might exist...
  44. ncbi Elimination of the disulfide bridge in the Rieske iron-sulfur protein allows assembly of the [2Fe-2S] cluster into the Rieske protein but damages the ubiquinol oxidation site in the cytochrome bc1 complex
    Torsten Merbitz-Zahradnik
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Biochemistry 42:13637-45. 2003
    ....
  45. ncbi Ubiquinol cytochrome c reductase (UQCRFS1) gene amplification in primary breast cancer core biopsy samples
    Yoko Ohashi
    Department of Obstetrics and Gynecology, University of South Carolina School of Medicine, Columbia, SC 29203, USA
    Gynecol Oncol 93:54-8. 2004
    ..The purpose of this study is to evaluate the significance of the ubiquinol cytochrome c reductase UQCRFS1 gene amplification in primary breast cancers...
  46. ncbi Purification of a cytochrome bc-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunity of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1
    Axel Niebisch
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    J Biol Chem 278:4339-46. 2003
    ..This indicates that the attachment of both heme groups to apo-cytochrome c(1) is not only required for the activity but also for the assembly and/or stability of the bc(1) complex...
  47. ncbi The iron-sulfur cluster of the Rieske iron-sulfur protein functions as a proton-exiting gate in the cytochrome bc(1) complex
    Buddha Gurung
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:24895-902. 2005
    ..It was speculated that in the normal catalytic cycle of the bc(1) complex, the [2Fe-2S] cluster may function as a proton-exiting gate...
  48. ncbi CA125 and UQCRFS1 FISH studies of ovarian carcinoma
    Saori J Kaneko
    Department of Obstetrics and Gynecology, University of South Carolina School of Medicine, Columbia, SC 29203, USA
    Gynecol Oncol 90:29-36. 2003
    ..As a control we chose BACs close to the chromosome 19 centromere. One of these BACs carries the gene UQCRFS1...
  49. ncbi The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc(1) complexes
    Tiago M Bandeiras
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica EAN, 2780 157 Oeiras, Portugal
    Biochim Biophys Acta 1787:37-45. 2009
    ..Based on these findings, we propose that the A. ambivalens cytochrome ba complex is analogous to the bc(1) complexes of bacteria and mitochondria, however with distinct subunits and heme types...
  50. pmc Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c
    Christian Lange
    Max Planck Institute for Biophysics, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 99:2800-5. 2002
    ..Obviously, cytochrome c reduction by the cytochrome bc1 complex can be regulated in response to respiratory conditions...
  51. pmc An electronic bus bar lies in the core of cytochrome bc1
    Monika Swierczek
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Science 329:451-4. 2010
    ..Free and unregulated distribution of electrons acts like a molecular-scale bus bar, a design often exploited in electronics...
  52. ncbi Molecular analysis of the cytochrome bc1-aa3 branch of the Corynebacterium glutamicum respiratory chain containing an unusual diheme cytochrome c1
    A Niebisch
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    Arch Microbiol 175:282-94. 2001
    ..Similar to the deltactaD mutant, the deltaqcrCAB mutant showed strongly impaired growth in glucose minimal medium, which indicates that the bc1-aa3 pathway is the main route of respiration under these conditions...
  53. ncbi Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer
    Sozanne R N Solmaz
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
    J Biol Chem 283:17542-9. 2008
    ..Four core interactions encircle the heme cofactors surrounded by variable interactions. The core interface may be a feature to gain specificity for formation of the reactive complex...
  54. ncbi Rieske business: structure-function of Rieske non-heme oxygenases
    Daniel J Ferraro
    Department of Biochemistry, University of Iowa Roy J and Lucille A Carver College of Medicine, 51 Newton Road, 4 403 BSB, Iowa City, IA 52242, USA
    Biochem Biophys Res Commun 338:175-90. 2005
    ..The orientation of binding of the substrate to the enzyme is hypothesized to control the substrate selectivity and regio-specificity of product formation...
  55. pmc Intermonomer electron transfer between the low-potential b hemes of cytochrome bc₁
    Pascal Lanciano
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States
    Biochemistry 50:1651-63. 2011
    ..Overall findings are discussed with respect to intra- and intermonomer interactions that take place during the catalytic turnover of cyt bc(1)...
  56. ncbi Reversible redox energy coupling in electron transfer chains
    Artur Osyczka
    The Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA
    Nature 427:607-12. 2004
    ....
  57. ncbi Control of oxidative phosphorylation by Complex I in rat liver mitochondria: implications for aging
    Barbara Ventura
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    Biochim Biophys Acta 1553:249-60. 2002
    ..Our results point out that age-related alterations of the mitochondrial functions are also present in tissues considered less prone to accumulate mitochondrial DNA mutations...
  58. ncbi The twin-arginine translocation pathway is necessary for correct membrane insertion of the Rieske Fe/S protein in Legionella pneumophila
    Emmy De Buck
    Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    FEBS Lett 581:259-64. 2007
    ..We conclude that the Tat pathway is necessary for correct membrane insertion of L. pneumophila PetA...
  59. ncbi Genetics and the specificity of the aging process
    Siegfried Hekimi
    Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada
    Science 299:1351-4. 2003
    ..Thus, the aging process may be more specific than previously anticipated on evolutionary grounds...
  60. ncbi Transcriptional activation of the alternative oxidase gene of the fungus Magnaporthe grisea by a respiratory-inhibiting fungicide and hydrogen peroxide
    H Yukioka
    Aburahi Laboratories, Shionogi and Co, Ltd, 1405 Gotanda, Koka, Shiga 520 3423, Japan
    Biochim Biophys Acta 1442:161-9. 1998
    ..grisea. Because we have previously shown that SSF-126 treatment of M. grisea mitochondria induced the generation of superoxide, active oxygen species are thought to be signal mediators to activate AOX gene transcription in M. grisea...
  61. ncbi Respiratory-chain diseases related to complex III deficiency
    Paule Benit
    INSERM, U676, Hopital Robert Debre, Paris, F 75019, France
    Biochim Biophys Acta 1793:181-5. 2009
    ..Finally, we discuss genetic and biochemical aspects that may help to understand complex III-associated diseases...
  62. ncbi Studies of the cytochrome subunits of menaquinone:cytochrome c reductase (bc complex) of Bacillus subtilis. Evidence for the covalent attachment of heme to the cytochrome b subunit
    J Yu
    Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 273:8860-6. 1998
    ..It is proposed that heme is also covalently attached to the cytochrome b subunit of b6f complexes of chloroplasts and cyanobacteria...
  63. ncbi Crystal structure of the terminal oxygenase component of biphenyl dioxygenase derived from Rhodococcus sp. strain RHA1
    Yutaka Furusawa
    Biological Information Research Center BIRC, National Institute of Advanced Industrial Science and Technology AIST, 2 41 6 Aomi, Koto ku, Tokyo 135 0064, Japan
    J Mol Biol 342:1041-52. 2004
    ..The cooperative actions of residues in the three groups seem to determine the substrate specificity of the enzyme...
  64. pmc Direct demonstration of half-of-the-sites reactivity in the dimeric cytochrome bc1 complex: enzyme with one inactive monomer is fully active but unable to activate the second ubiquinol oxidation site in response to ligand binding at the ubiquinone reducti
    Michela Castellani
    Institute of Biochemistry, Molecular Genetics, Goethe University and Cluster of Excellence Macromolecular Complexes Frankfurt am Main, D 60438 Frankfurt am Main, Germany
    J Biol Chem 285:502-10. 2010
    ....
  65. ncbi Controlling the functionality of cytochrome c(1) redox potentials in the Rhodobacter capsulatus bc(1) complex through disulfide anchoring of a loop and a beta-branched amino acid near the heme-ligating methionine
    A Osyczka
    The Johnson Research Foundation, Department of Biochemistry and Biophysics, Plant Science Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 40:14547-56. 2001
    ..The disulfide anchored loop and betaXM motifs appear to be two independent but nonadditive strategies to control the integrity of the heme-binding pocket and raise cytochrome c midpoint potentials...
  66. ncbi Architecture of active mammalian respiratory chain supercomplexes
    Eva Schäfer
    Department of Chemistry, Physical Biochemistry, Darmstadt University of Technology, Petersenstrasse 22, D 64287 Darmstadt, Germany
    J Biol Chem 281:15370-5. 2006
    ..Detailed knowledge of the architecture of the active supercomplexes is a prerequisite for a deeper understanding of energy conversion by mitochondria in mammals...
  67. ncbi Subcomplexes of human ATP synthase mark mitochondrial biosynthesis disorders
    Rosalba Carrozzo
    Unit of Molecular Medicine, Bambino Gesù Hospital and Research Institute, Rome, Italy
    Ann Neurol 59:265-75. 2006
    ..Methods: We describe biochemically and clinically relevant aspects of mitochondrial ATP synthase, the enzyme that supplies most ATP for the cells energy demand...
  68. ncbi Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc1 complex
    Gregory Engstrom
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Biochemistry 42:2816-24. 2003
    ..The interaction of yeast Ru-39-Cc with yeast cytochrome bc1 is stronger than that of horse Ru-39-Cc with bovine cytochrome bc1, suggesting that nonpolar interactions are stronger in the yeast system...
  69. pmc Plasmon waveguide resonance spectroscopic evidence for differential binding of oxidized and reduced Rhodobacter capsulatus cytochrome c2 to the cytochrome bc1 complex mediated by the conformation of the Rieske iron-sulfur protein
    S Devanathan
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 46:7138-45. 2007
    ....
  70. ncbi The Rieske protein: a case study on the pitfalls of multiple sequence alignments and phylogenetic reconstruction
    Evelyne Lebrun
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Institut de Biologie Structurale et Microbiologie IFR, Marseille, France
    Mol Biol Evol 23:1180-91. 2006
    ..The obtained results are discussed against the background of hypotheses stipulating pervasive lateral gene transfer in prokaryotes...
  71. ncbi 2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction
    Berta Maria Martins
    Laboratorium Proteinkristallographie, Universitat Bayreuth, Germany
    Structure 13:817-24. 2005
    ..This creates an additional coordination site at the mononuclear Fe(II) ion and can open a pathway for dioxygen to bind in the substrate-containing active site...
  72. pmc The Qo site of the mitochondrial complex III is required for the transduction of hypoxic signaling via reactive oxygen species production
    Eric L Bell
    Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA
    J Cell Biol 177:1029-36. 2007
    ..These results provide genetic and pharmacologic evidence that the Qo site of complex III is required for the transduction of hypoxic signal by releasing ROS to stabilize the HIF-1alpha protein...
  73. pmc Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
    C Lange
    , Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt, Germany
    EMBO J 20:6591-600. 2001
    ..Site-directed mutagenesis of ligating residues confirmed the importance of the phosphatidylinositol- and cardiolipin-binding sites...
  74. pmc LYRM7/MZM1L is a UQCRFS1 chaperone involved in the last steps of mitochondrial Complex III assembly in human cells
    Ester Sanchez
    Unidad de Investigación Traslacional, Hospital Universitario Miguel Servet, Zaragoza, Spain
    Biochim Biophys Acta 1827:285-93. 2013
    ..Thus, LYRM7/MZM1L is a novel human CIII assembly factor involved in the UQCRFS1 insertion step, which enables formation of the mature and functional CIII enzyme...
  75. pmc The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria
    Aaron Atkinson
    University of Utah Health Sciences Center, Department of Medicine, Salt Lake City, UT 84132, USA
    Mol Cell Biol 31:3988-96. 2011
    ....
  76. ncbi Differences in assembly or stability of complex I and other mitochondrial OXPHOS complexes in inherited complex I deficiency
    Cristina Ugalde
    Nijmegen Center for Mitochondrial Disorders, Department of Pediatrics, University Medical Center, Nijmegen, The Netherlands
    Hum Mol Genet 13:659-67. 2004
    ..This will help us to point more selectively to candidate genes for pathogenic mutations that could lead to an isolated complex I defect...
  77. ncbi New archaebacterial genes coding for redox proteins: implications for the evolution of aerobic metabolism
    J Castresana
    European Molecular Biology Laboratory, Heidelberg, Germany
    J Mol Biol 250:202-10. 1995
    ..A phylogenetic analysis of the new protein sequences gives support to the view that an elaborate aerobic respiratory chain was already present in the last common ancestor of all living organisms...
  78. ncbi Cellular pathophysiological consequences of BCS1L mutations in mitochondrial complex III enzyme deficiency
    Maria Moran
    Centro de Investigacion, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain
    Hum Mutat 31:930-41. 2010
    ..The observed structural alterations were independent of the respiratory chain function and ROS production. Our results provide new insights into the role of pathogenic BCS1L mutations in mitochondrial function and dynamics...
  79. ncbi Essential role of complex II of the respiratory chain in hypoxia-induced ROS generation in the pulmonary vasculature
    Renate Paddenberg
    Institute of Anatomy and Cell Biology, Justus Liebig University, 35385 Giessen, Germany
    Am J Physiol Lung Cell Mol Physiol 284:L710-9. 2003
    ..Presumably, its catalytic activity switches from succinate dehydrogenase to fumarate reductase at reduced oxygen tension, thereby modulating the directionality of the electron flow...
  80. ncbi Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal rieske [2Fe-2S] center
    Toshio Iwasaki
    Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    J Am Chem Soc 126:13902-3. 2004
    ..These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron-proton transfer reactions...
  81. ncbi Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system
    Yuji Ashikawa
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Structure 14:1779-89. 2006
    ..Additionally, conformational changes upon binding carbazole resulted in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site...
  82. ncbi The naphthoquinol oxidizing cytochrome bc1 complex of the hyperthermophilic knallgasbacterium Aquifex aeolicus: properties and phylogenetic relationships
    Michael Schutz
    Laboratoire de Bioénergétique et Ingénierie des Protéines UPR9036, CNRS, 31, Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Biochemistry 42:10800-8. 2003
    ..The Aquifex enzyme was found to represent the only extant naphthoquinol oxidizing true cyt bc(1) complex described so far. An improved scenario for the phylogenetic positioning of the Aquifex cyt bc(1) complex is proposed...
  83. ncbi Structure of the cytochrome b6f complex of oxygenic photosynthesis: tuning the cavity
    Genji Kurisu
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    Science 302:1009-14. 2003
    ..The motion of the Rieske iron-sulfur protein extrinsic domain, essential for electron transfer, must also be different in the b6f complex...
  84. pmc Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b6-f complex: position of the cytochrome b hemes in the membrane
    W R Widger
    Proc Natl Acad Sci U S A 81:674-8. 1984
    ..In addition, the 17-kDa protein of the chloroplast b6-f complex appears to contain one or more of the functions of the COOH-terminal end of the mitochondrial cytochrome b polypeptide...
  85. ncbi A novel Rieske iron-sulfur protein from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum: sequencing of the gene, expression in E. coli and characterization of the protein
    T Henninger
    Institut fur Biochemie, Medizinische Universitat zu Lubeck, Germany
    J Bioenerg Biomembr 31:119-28. 1999
    ....
  86. ncbi Haloarcula marismortui cytochrome b-561 is encoded by the narC gene in the dissimilatory nitrate reductase operon
    Katsuhiko Yoshimatsu
    Department of Biological Science, Faculty of Science, Shizuoka University, 836 Ohya, Suruga ku, Shizuoka, 422 8529, Japan
    Extremophiles 11:41-7. 2007
    ..The sequence of the cytochrome was homologous with that of the cytochrome b subunit of respiratory cytochrome bc. A possibility that the cytochrome bc and the NarGH constructed a supercomplex was discussed...
  87. ncbi Reconstitution of mitochondrial processing peptidase from the core proteins (subunits I and II) of bovine heart mitochondrial cytochrome bc(1) complex
    K Deng
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 276:6499-505. 2001
    ..The aromatic group, rather than the hydroxyl group, at Tyr(57) of core I is essential for reconstitutive activity...
  88. ncbi The Rieske/cytochrome b complex of Heliobacteria
    A L Ducluzeau
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Institut de Biologie Structurale et Microbiologie, Unité Propre de Recherche 9036, Centre National de la Recherche Scientifique, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1777:1140-6. 2008
    ..In contrast to the case of b(6)f complex, a strong axial ligand to the heme is present, most probably a protonatable amino acid residue...
  89. ncbi The "green" phylogenetic clade of Rieske/cytb complexes
    W Nitschke
    BIP, Centre National de la Recherche Scientifique, UPR9036, IFR88, 31 Chemin Joseph Aiguier, Marseille, France
    Photosynth Res 104:347-55. 2010
    ..Structural and functional idiosyncrasies are (re-)interpreted in the framework of evolutionary biology and more specifically evolutionary bioenergetics...
  90. ncbi Analysis of suppressor mutation reveals long distance interactions in the bc(1) complex of Saccharomyces cerevisiae
    G Brasseur
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, Marseilles, France
    Biochim Biophys Acta 1506:89-102. 2001
    ..Surprisingly, reversion at position 260 was located 13 A away from the original mutation and revealed a long distance interaction in the yeast bc(1) complex...
  91. ncbi Critical role of complex III in the early metabolic changes following myocardial infarction
    Lisa C Heather
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK
    Cardiovasc Res 85:127-36. 2010
    ..Therefore, we investigated cardiac mitochondrial metabolism shortly after infarction...
  92. pmc Modifications of protein environment of the [2Fe-2S] cluster of the bc1 complex: effects on the biophysical properties of the rieske iron-sulfur protein and on the kinetics of the complex
    Sangmoon Lhee
    Center for Biophysics and Computational Biology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 285:9233-48. 2010
    ..The proton-first-then-electron model proposed accounts naturally for the effects of mutation on the overall reaction...
  93. ncbi Molecular basis of resistance to cytochrome bc1 inhibitors
    Nick Fisher
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, UK
    FEMS Yeast Res 8:183-92. 2008
    ..Four mutations that are of particular importance in medicine and in agriculture are briefly reviewed and described in more detail and the molecular basis of resistance and of evolution of the mutations is discussed succinctly...
  94. ncbi QO site deficiency can be compensated by extragenic mutations in the hinge region of the iron-sulfur protein in the bc1 complex of Saccharomyces cerevisiae
    Gael Brasseur
    Laboratoire de Bioenergetique et Ingenierie des Proteines, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 279:24203-11. 2004
    ....
  95. ncbi Oxygen sensing requires mitochondrial ROS but not oxidative phosphorylation
    Joslyn K Brunelle
    Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Cell Metab 1:409-14. 2005
    ..These findings provide genetic evidence that oxygen sensing is dependent on mitochondrial-generated reactive oxygen species (ROS) but independent of oxidative phosphorylation...
  96. pmc Discrimination between two possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc₁. Implications for the mechanism of superoxide production
    Marcin Sarewicz
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biochim Biophys Acta 1797:1820-7. 2010
    ..Isolation of this reaction sequence from multiplicity of possible reactions at Q(o) helps to better understand conditions under which complex III might contribute to ROS generation in vivo...
  97. pmc Cytochrome bc1-cy fusion complexes reveal the distance constraints for functional electron transfer between photosynthesis components
    Dong Woo Lee
    Department of Biology, Plant Science Institute
    J Biol Chem 283:13973-82. 2008
    ..These findings support the notion that membrane-bound Ps components form large, active structural complexes that are "hardwired" for cyclic ET...
  98. ncbi Proton-coupled electron transfer at the Q(o) site: what type of mechanism can account for the high activation barrier?
    A R Crofts
    Department of Biochemistry, University of Illinois, Urbana 61801, USA
    Biochim Biophys Acta 1459:456-66. 2000
    ....
  99. ncbi Modeling the molecular basis of atovaquone resistance in parasites and pathogenic fungi
    Jacques J Kessl
    Center for Retrovirus Research, College of Pharmacy, Ohio State University, Columbus, OH 43210, USA
    Trends Parasitol 23:494-501. 2007
    ..To better understand the molecular basis of drug resistance, we have developed the yeast and bovine bc1 complexes as surrogates to model the molecular interaction of atovaquone with human and resistant pathogen enzymes...
  100. pmc Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis
    Monika Czapla
    Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Protein Eng Des Sel 25:15-25. 2012
    ....
  101. pmc Inhibitory effects of nitric oxide on invasion of human cancer cells
    Feng Wang
    Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA
    Cancer Lett 257:274-82. 2007
    ..Our findings suggest that NO inhibits invasion of cancer cells via both inhibition of HIF-1, and impairment of mitochondria...

Research Grants44

  1. BIOENERGETICS OF THE CYTOCHROME BC1 COMPLEX
    DIANA BEATTIE; Fiscal Year: 2001
    ..performed to determine whether charged amino acid residues ar required for efficient assembly of the ISP into the bc1 complex and for enzymatic activity. ..
  2. Molecular Pathogenesis of Coenzyme Q10 Deficiency
    Michio Hirano; Fiscal Year: 2012
    ..Aim 4: In collaboration with Professor Pl[unreadable]cido Navas, University of Sevilla, Spain, we will characterize CoQ10 biosynthetic genes and mutations in yeast. ..
  3. Discovery of Novel Antitumor Agents Effective Against Pancreatic Cancer
    Amy E Wright; Fiscal Year: 2012
    ..The compounds discovered under this project may be used as drugs themselves, modified to provide drugs with improved pharmacological properties or be used as biochemical tools to further understand pancreatic cancer. ..
  4. Charge Transfer Reactions of the Cytochrome bc1 Complex
    Colin A Wraight; Fiscal Year: 2011
    ..Several chronic and congenital diseases (myopathies) also arise from malfunctioning bc1 complex. Finally, this activity is a target for new therapeutic agents, especially for fungi and parasitic protists. ..
  5. Mitochondrial Functions in Malaria Parasites
    Akhil B Vaidya; Fiscal Year: 2013
    ..These studies have the potential to be highly valuable in developing strategies for chemotherapeutic intervention affecting validated targets of malaria parasites. ..
  6. Structure of reaction intermediates in the bc1 complex
    Sergei A Dikanov; Fiscal Year: 2010
    ..The central role of bc1 complex plays out in many medical scenarios in which defects lead to pathology, among them cellular death through ROS-mediated damage, mitochondrial myopathies, and apoptosis, and in drug targeting. ..
  7. BACTERIAL CYTOCHROME BC1: STRUCTURE, FUNCTION, BIOGENESIS
    M FEVZI DALDAL; Fiscal Year: 2013
    ..Malfunction of these enzymes induce multiple human illnesses, extending from maternally inherited mitochondrial diseases to neuromuscular degenerative disorders, as well as cancer and aging. ..
  8. Cardiolipin Function in Mitochondrial Electron-Transfer Complexes
    Neal C Robinson; Fiscal Year: 2011
    ..Successful completion of this project will determine the absolute requirement for cardiolipin and help us understand its pathophysiological importance. ..
  9. REACTION MECHANISM OF MYOCARDIAC UBIQUINONE PROTEINS
    Chang An Yu; Fiscal Year: 1999
    ..This information will be useful in pharmaceutical investigations of cytotoxicity and the aging process. ..
  10. Molecular Analysis of the Mitochondrial ADP/ATP Carrier Protein Complexes
    Rosemary Stuart; Fiscal Year: 2009
    ....
  11. 1999 GORDON CONFERENCE ON BIOENERGETICS
    William Allison; Fiscal Year: 1999
    ..abstract_text> ..
  12. STRUCTURAL ANALYSIS OF MEMBRANE PROTEINS
    Maureen McEnery; Fiscal Year: 2000
    ..abstract_text> ..
  13. REDOX ACTIVITY OF THE PULMONARY ENDOTHELIAL SURFACE
    MARILYN MERKER; Fiscal Year: 2008
    ....
  14. Mechanisms of Replicative Senescence of lung cells during hypoxia.
    Navdeep Chandel; Fiscal Year: 2007
    ..Collectively these studies will provide new information regarding mechanisms underlying replicative senescence and the aging process. [unreadable] [unreadable] [unreadable]..
  15. Mechanisms of HIF activation in pulmonary vasculature.
    Navdeep Chandel; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  16. 3D CRYSTALS OF CYTOCHROME REDUCTASE, A MEMBRANE PROTEIN
    EDWARD BERRY; Fiscal Year: 2006
    ..5) Structural studies of functionally relevant mutant bacterial and yeast complexes. ..
  17. 3D Crystal Structure: Vertebrate Mitochondria Complex II
    EDWARD BERRY; Fiscal Year: 2005
    ..Finally (e) we want to crystallize a related complex n from an organism amenable to site-directed mutagenesis. ..
  18. MECHANISM OF RESPIRATION AND ENERGY TRANSDUCTION
    Bernard Trumpower; Fiscal Year: 2009
    ..abstract_text> ..
  19. Mechanisms for hypoxic Ca2+ release in pulmonary artery myocytes
    Yong Xiao Wang; Fiscal Year: 2010
    ..The findings from this proposal will enhance our understanding of cellular molecular mechanisms for hypoxic [Ca2+]i rise in PASMCs and associated HPV, and may lead to identify potential novel targets to treat pulmonary hypertension. ..
  20. MOLECULAR GENETICS OF MAMMALIAN CYTOCHROME C OXIDASE
    Lawrence Grossman; Fiscal Year: 2001
    ....
  21. Modulation of ATP synthesis in mitochondrial disorders
    Giovanni Manfredi; Fiscal Year: 2008
    ....
  22. ELECTRON-TRANSFER PROTEINS
    FRANCIS S contact MILLETT; Fiscal Year: 2010
    ..In the proposed studies, new ruthenium photoexcitation methods will be developed to study electron transfer reactions important to human health. ..
  23. Preventive of Bile Acid-Induced Colonic Oxidative DNA Damage
    Claire Margaret Payne; Fiscal Year: 2010
    ..129 mice) developed in our laboratory;2) NOS-/- k.o./DOC mouse model;3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory;4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
  24. CENTER FOR PROTEIN STRUCTURE AND FUNCTION
    Francis Millett; Fiscal Year: 2009
    ..abstract_text> ..
  25. Mitochondrial sites of reactive oxygen species generation in cells.
    Martin D Brand; Fiscal Year: 2010
    ....
  26. Heterogeneity of hypoxic Ca2+ release in pulmonary and *
    Yong Xiao Wang; Fiscal Year: 2006
    ..The proposed studies are also of fundamental physiological significance with respect to the regulation of Ca2+ release in other cell types. ..
  27. Oral Yeast Carriage and Salivary Antifungal Activity
    EVA HELMERHORST; Fiscal Year: 2008
    ..The experiments outlined here will provide the basis for a better understanding of antifungal mechanisms exerted by saliva in the oral cavity. [unreadable] [unreadable] [unreadable]..
  28. RECEPTOR MEDIATED OXIDATIVE STRESS
    HOWARD SHERTZER; Fiscal Year: 2003
    ..These studies will improve our understanding of how PHAHs such as TCDD generate oxidative stress and mutagenesis, leading to better models for risk assessment and novel strategies for intervention in PHAH-mediated toxicity. ..
  29. Meeting:Society for Integrative and Comparative Biology
    Neil Blackstone; Fiscal Year: 2004
    ..Ultimately, this work will allow the power of genomics to be fully realized in biology and medicine. ..
  30. CEREBRAL MITOCHONDRIAL METABOLISM IN NEURODEGENERATION
    William Powers; Fiscal Year: 2004
    ..If such studies yield consistent results, they will establish the basis for the utilization of platelet rnitochondrial function assays to monitor cerebral mitochondrial metabolism. ..
  31. Mitochondrial control of neuronal excitotoxicity
    David Nicholls; Fiscal Year: 2005
    ..4. To test the hypothesis that mitochondria at specific intracellular locations may be selectively vulnerable to glutamate excitotoxicity because of their proximity to NMDA receptors, or their location in regions of high-energy demand. ..
  32. ALTERNATIVE MECHANISMS OF UGA CODON RECOGNITION
    Marla Berry; Fiscal Year: 2005
    ..Continuing studies of this process will surely unveil new and exciting information about the complex workings of the translation machinery. ..
  33. Oxidants, Mitochondria,and Renal Ischemia/Reperfusion
    LEE MACMILLAN CROW; Fiscal Year: 2005
    ..This information will be helpful in determining possible therapeutic interventions for improving current techniques in renal preservation and transplantation. ..
  34. Molecular analysis of the dimeric F1/F0-ATP synthase
    Rosemary Stuart; Fiscal Year: 2006
    ....
  35. Aging-Related Models and Mitochondrial Bioenergetics
    David Nicholls; Fiscal Year: 2006
    ..3. To indicate therapeutic strategies whereby the deleterious consequences of aging-related changes in mitochondrial function can be minimized. ..
  36. Cytochrome C Oxidase I:A Biomarker for Colon Cancer Risk
    Claire Payne; Fiscal Year: 2006
    ..Various molecular and cellular techniques will be used in these studies. ..
  37. Receptor-mediated Colicin Import
    WILLIAM CRAMER; Fiscal Year: 2007
    ..2) The pathway of membrane insertion of the E1 channel domain, relative to the time course of other unfolding events, will be examined by time-resolved FRET using a large set of single Trp mutants. [unreadable] [unreadable]..
  38. Novel Signaling for Ca2+ and Release in Airway Myocytes
    Yong Xiao Wang; Fiscal Year: 2007
    ..6, and/or RyRs are ubiquitously expressed in a variety of cell types, the mechanistic findings from the proposed study will have general biological and pathological significance for various Ca2+-mediated cellular effects. ..
  39. Glutathione in Environmental Toxicity and Disease
    HOWARD SHERTZER; Fiscal Year: 2007
    ..Our proposed research should therefore help focus future genotype-phenotype association studies between the appropriate DNA variant sites in the GCLC and GCLM genes and disease. ..
  40. SELENOPROTEIN P FUNCTION AND REGULATION OF EXPRESSION
    Marla Berry; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  41. 2007 Molecular &Cellular Bioenergetics
    Fevzi Daldal; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  42. Structure-Function of Hetero-oligomeric Integral Membrane Proteins
    WILLIAM CRAMER; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  43. ATP SYNTHESIS IN MITOCHONDRIAL DISORDERS
    Giovanni Manfredi; Fiscal Year: 2003
    ..Allotopic expression of the recoded wild-type genes should partially restore the APT synthetic function in mutant cells. ..