photosystem i protein complex

Summary

Summary: A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.

Top Publications

  1. ncbi Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution
    P Jordan
    Institut für Chemie Kristallographie, Freie Universitat Berlin, D 14195 Berlin, Takustrasse 6, Germany
    Nature 411:909-17. 2001
  2. ncbi Cyclic electron flow around photosystem I is essential for photosynthesis
    Yuri Munekage
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Nature 429:579-82. 2004
  3. ncbi Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration
    L Cournac
    Commissariat à l Energie Atomique CEA Cadarache, Départment d Ecophysiologie Végétale et de Microbiologie DEVM, laboratoire d écophysiologie de la photosynthèse, 13108 Saint Paul Lez Durance, France
    J Biol Chem 275:17256-62. 2000
  4. ncbi Chlororespiration and cyclic electron flow around PSI during photosynthesis and plant stress response
    Dominique Rumeau
    Laboratoire d Ecophysiologie Moléculaire des Plantes, CEA Cadarache, DSV, IBEB, SBVME, UMR 6191 CNRS CEA Université Aix Marseilles, Saint Paul lez Durance F 13108, France
    Plant Cell Environ 30:1041-51. 2007
  5. pmc Cyclic electron flow around photosystem I in C(3) plants. In vivo control by the redox state of chloroplasts and involvement of the NADH-dehydrogenase complex
    Thierry Joet
    Commissariat à l Energie Atomique Cadarache, Département d Ecophysiologie Végétale et de Microbiologie, Unité Mixte de Recherche 163 Centre National de la Recherche Scientifique, F 13108 Saint Paul lez Durance, France
    Plant Physiol 128:760-9. 2002
  6. doi Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis
    Masakazu Iwai
    Institute of Low Temperature Science, Hokkaido University, Sapporo 060 0819, Japan
    Nature 464:1210-3. 2010
  7. doi Dynamics of reversible protein phosphorylation in thylakoids of flowering plants: the roles of STN7, STN8 and TAP38
    Paolo Pesaresi
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, I 20133 Milano, Italy
    Biochim Biophys Acta 1807:887-96. 2011
  8. doi A novel photosynthetic strategy for adaptation to low-iron aquatic environments
    Devendra Chauhan
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States
    Biochemistry 50:686-92. 2011
  9. ncbi Photoinhibition of photosystem I
    Henrik Vibe Scheller
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, 1871, Frederiksberg C, Copenhagen, Denmark
    Planta 221:5-8. 2005
  10. ncbi Molecular recognition in thylakoid structure and function
    J F Allen
    Plant Biochemistry, Lund University, Box 117, SE 221 00, Lund, Sweden
    Trends Plant Sci 6:317-26. 2001

Research Grants

Detail Information

Publications313 found, 100 shown here

  1. ncbi Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution
    P Jordan
    Institut für Chemie Kristallographie, Freie Universitat Berlin, D 14195 Berlin, Takustrasse 6, Germany
    Nature 411:909-17. 2001
    ..The structural information on the proteins and cofactors and their interactions provides a basis for understanding how the high efficiency of photosystem I in light capturing and electron transfer is achieved...
  2. ncbi Cyclic electron flow around photosystem I is essential for photosynthesis
    Yuri Munekage
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Nature 429:579-82. 2004
    ..Here we have constructed mutants in Arabidopsis thaliana in which both PSI cyclic pathways are impaired, and present evidence that cyclic flow is essential for efficient photosynthesis...
  3. ncbi Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration
    L Cournac
    Commissariat à l Energie Atomique CEA Cadarache, Départment d Ecophysiologie Végétale et de Microbiologie DEVM, laboratoire d écophysiologie de la photosynthèse, 13108 Saint Paul Lez Durance, France
    J Biol Chem 275:17256-62. 2000
    ..Clues to the identity of the protein implied in this process are given by pharmacological and immunological similarities with a protein (IMMUTANS) identified in Arabidopsis chloroplasts...
  4. ncbi Chlororespiration and cyclic electron flow around PSI during photosynthesis and plant stress response
    Dominique Rumeau
    Laboratoire d Ecophysiologie Moléculaire des Plantes, CEA Cadarache, DSV, IBEB, SBVME, UMR 6191 CNRS CEA Université Aix Marseilles, Saint Paul lez Durance F 13108, France
    Plant Cell Environ 30:1041-51. 2007
    ..In response to severe stress conditions, PTOX associated to the NDH and/or the PGR5 pathway may also limit electron pressure on PSI acceptor and prevent PSI photoinhibition...
  5. pmc Cyclic electron flow around photosystem I in C(3) plants. In vivo control by the redox state of chloroplasts and involvement of the NADH-dehydrogenase complex
    Thierry Joet
    Commissariat à l Energie Atomique Cadarache, Département d Ecophysiologie Végétale et de Microbiologie, Unité Mixte de Recherche 163 Centre National de la Recherche Scientifique, F 13108 Saint Paul lez Durance, France
    Plant Physiol 128:760-9. 2002
    ..We conclude that the Ndh complex is required for rapid electron cycling around PS I...
  6. doi Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis
    Masakazu Iwai
    Institute of Low Temperature Science, Hokkaido University, Sapporo 060 0819, Japan
    Nature 464:1210-3. 2010
    ..1). Thus, formation and dissociation of the PSI-LHCI-LHCII-FNR-Cyt bf-PGRL1 supercomplex not only controlled the energy balance of the two photosystems, but also switched the mode of photosynthetic electron flow...
  7. doi Dynamics of reversible protein phosphorylation in thylakoids of flowering plants: the roles of STN7, STN8 and TAP38
    Paolo Pesaresi
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, I 20133 Milano, Italy
    Biochim Biophys Acta 1807:887-96. 2011
    ..This article is part of a Special Issue entitled: Regulation of Electron Transport in Chloroplasts...
  8. doi A novel photosynthetic strategy for adaptation to low-iron aquatic environments
    Devendra Chauhan
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States
    Biochemistry 50:686-92. 2011
    ..Excitation trapping and electron transfer are highly efficient, allowing cyanobacteria to avoid oxidative stress. This mechanism may be a major factor used by cyanobacteria to successfully adapt to modern low-Fe environments...
  9. ncbi Photoinhibition of photosystem I
    Henrik Vibe Scheller
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, 1871, Frederiksberg C, Copenhagen, Denmark
    Planta 221:5-8. 2005
  10. ncbi Molecular recognition in thylakoid structure and function
    J F Allen
    Plant Biochemistry, Lund University, Box 117, SE 221 00, Lund, Sweden
    Trends Plant Sci 6:317-26. 2001
    ..High-resolution structural description of membrane protein-protein interactions is now required to understand thylakoid structure and regulation of photosynthesis...
  11. ncbi Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP
    Gert Schansker
    Bioenergetics Laboratory, University of Geneva, Chemin des Embrouchis 10, CH 1254 Jussy, Geneva, Switzerland
    Biochim Biophys Acta 1706:250-61. 2005
    ..3 s. Further, alternative explanations of the IP phase that have been proposed in the literature are discussed...
  12. ncbi Effects of iron limitation on the expression of metabolic genes in the marine cyanobacterium Trichodesmium erythraeum IMS101
    Tuo Shi
    Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901, USA
    Environ Microbiol 9:2945-56. 2007
    ..The observed functional and compositional alterations represent the compromises in gene expression and acclimation capacity between two basic metabolic pathways competing for iron when it is limiting...
  13. ncbi Structure and function of photosystems I and II
    Nathan Nelson
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Annu Rev Plant Biol 57:521-65. 2006
    ....
  14. pmc The high light-inducible polypeptides stabilize trimeric photosystem I complex under high light conditions in Synechocystis PCC 6803
    Qiang Wang
    Department of Applied Science, University of Arkansas, Little Rock, Arkansas 72204, USA
    Plant Physiol 147:1239-50. 2008
    ..These results suggest that the HLIPs stabilize PSI trimers, interact with Slr1128, and protect cells under HL conditions...
  15. pmc Photosystem I of Chlamydomonas reinhardtii contains nine light-harvesting complexes (Lhca) located on one side of the core
    Bartlomiej Drop
    Department of Biophysical Chemistry, Groningen Biological Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 286:44878-87. 2011
    ..The analysis of several subcomplexes with reduced antenna size allows determination of the position of Lhca2 and Lhca9 and leads to a proposal for a model of the organization of the Lhcas within the PSI-LHCI supercomplex...
  16. doi Cyclic electron flow around photosystem I in unicellular green algae
    Jean Alric
    UMR 7141, CNRS et Université Pierre et Marie Curie Paris VI, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Photosynth Res 106:47-56. 2010
    ..These ultrastructural changes have been proposed to further enhance cyclic electron flow by increasing PSI antenna size, and forming PSI-cyt b(6)f supercomplexes. These hypotheses are discussed in light of recently published data...
  17. ncbi Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance
    Shang Jing Guo
    College of Life Sciences, Shandong Agricultural University, Tai an, PR China
    J Plant Physiol 164:126-36. 2007
    ..These results suggest that CP sHSP protein plays an important role in protection of PSII and PSI during chilling stress under low irradiance...
  18. pmc Isolation and characterization of homodimeric type-I reaction center complex from Candidatus Chloracidobacterium thermophilum, an aerobic chlorophototroph
    Yusuke Tsukatani
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 287:5720-32. 2012
    ..4 chlorophyll a(PD), and 1.6 Zn-bacteriochlorophyll a(P)' molecules per P840 (12.8:8.0:2.0). The possible functions of the Zn-bacteriochlorophyll a(P)' molecules and the carotenoid-binding protein are discussed...
  19. ncbi Revealing the structure of the photosystem II chlorophyll binding proteins, CP43 and CP47
    J Barber
    Biochemistry Department, Wolfson Laboratories, Imperial College of Science, Technology and Medicine, SW7 2AY, London, UK
    Biochim Biophys Acta 1459:239-47. 2000
    ..Improved structural resolution and reconsiderations of the orientation of the porphyrin rings will allow further refinement of the model...
  20. ncbi CO2 response of cyclic electron flow around PSI (CEF-PSI) in tobacco leaves--relative electron fluxes through PSI and PSII determine the magnitude of non-photochemical quenching (NPQ) of Chl fluorescence
    Chikahiro Miyake
    Research Institute of Innovative Technology for the Earth, 9 2 Kizugawadai, Kizu cho, Soraku gun, Kyoto, 619 0292 Japan
    Plant Cell Physiol 46:629-37. 2005
    ..The main physiological function of CEF-PSI in photosynthesis of higher plants is discussed...
  21. doi Identification of a specific fucoxanthin-chlorophyll protein in the light harvesting complex of photosystem I in the diatom Cyclotella meneghiniana
    Thomas Veith
    Institute for Molecular Bio Sciences, Johann Wolfgang Goethe University, SiesmayerstraBe 70, D 60323 Frankfurt am Main, Germany
    Biochim Biophys Acta 1787:905-12. 2009
    ..Thus, an association of FCPb and PSI is assumed...
  22. ncbi Probing the structure of Lhca3 by mutation analysis
    Milena Mozzo
    Istituto di Biofisica CNR C o ITC via Sommarvie 18 38100 Povo Trento, Italy
    Biochim Biophys Acta 1757:1607-13. 2006
    ..Evidence is also shown for the presence in Lhca3 of chlorophylls in sites 1013 and 1023...
  23. ncbi Inhibition of photosystems I and II and enhanced back flow of photosystem I electrons in cucumber leaf discs chilled in the light
    S J Kim
    Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT 2601, Australia
    Plant Cell Physiol 42:842-8. 2001
    ..We suggest that promotion of controlled charge recombination and cyclic electron flow round PSI during chilling of leaves in the light may partly prevent further damage to both photosystems...
  24. doi Protein-protein interactions by molecular modeling and biochemical characterization of PSI-LHCI supercomplexes from Chlamydomonas reinhardtii
    Venkateswarlu Yadavalli
    Department of Biochemistry, University of Hyderabad, Hyderabad, India 500046
    Mol Biosyst 7:3143-51. 2011
    ..Thus, our 3D model may give valid structural information of the PSI-LHCI arrangement and its physiological role in C. reinhardtii...
  25. doi Novel insights into plant light-harvesting complex II phosphorylation and 'state transitions'
    Mikko Tikkanen
    Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, FIN 20014 Turku, Finland
    Trends Plant Sci 16:126-31. 2011
    ....
  26. pmc IsiA is required for the formation of photosystem I supercomplexes and for efficient state transition in synechocystis PCC 6803
    Qiang Wang
    Department of Applied Science, University of Arkansas, Little Rock, Arkansas, United States of America
    PLoS ONE 5:e10432. 2010
    ..These results demonstrated that IsiA is required for the formation of PSI trimers and other higher complexes, and that IsiA is critical for efficient state transition...
  27. doi Selective excitation of photosystems in chloroplasts inside plant leaves observed by near-infrared laser-based fluorescence spectral microscopy
    Makoto Hasegawa
    Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo ku, Kyoto, 606 8502 Japan
    Plant Cell Physiol 51:225-38. 2010
    ..A new methodology using an NIR laser to detect the PSI/PSII ratio in single chloroplasts in leaves at room temperature is described...
  28. doi The PsaE subunit of photosystem I prevents light-induced formation of reduced oxygen species in the cyanobacterium Synechocystis sp. PCC 6803
    Robert Jeanjean
    Laboratoire de Chimie Bacterienne, CNRS UPR 9043, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, F 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1777:308-16. 2008
    ..PsaE-deficient Synechocystis cells can counteract the chronic photoreduction of oxygen by increasing their capacity to detoxify reactive oxygen species...
  29. doi Characterization of PSI recovery after chilling-induced photoinhibition in cucumber (Cucumis sativus L.) leaves
    Zishan Zhang
    State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai an, 271018, China
    Planta 234:883-9. 2011
    ..Under a given photon flux density, faster recovery of PSII compared to PSI was detrimental to the recovery of PSI or even to the whole photosystem...
  30. ncbi Mutants for photosystem I subunit D of Arabidopsis thaliana: effects on photosynthesis, photosystem I stability and expression of nuclear genes for chloroplast functions
    Anna Ihnatowicz
    Abteilung für Pflanzenzüchtung und Ertragsphysiologie, Max Planck Institut fur Zuchtungsforschung, Carl von Linne Weg 10, D 50829 Köln, Germany
    Plant J 37:839-52. 2004
    ..Increased dosage of PsaD2 in the psad1-1 background restores the WT phenotype, indicating that PSI-D1 and PSI-D2 have redundant functions...
  31. ncbi Daddy, where did (PS)I come from?
    F Baymann
    Laboratoire de Bioenergetique et Ingenierie des Proteines, Institut de Biologie Structurale et Microbiologie, Marseille, France
    Biochim Biophys Acta 1507:291-310. 2001
    ..An evolutionary model trying to rationalise available data is presented...
  32. pmc Interaction between starch breakdown, acetate assimilation, and photosynthetic cyclic electron flow in Chlamydomonas reinhardtii
    Xenie Johnson
    UMR 7141, CNRS et Université Pierre et Marie Curie Paris VI, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
    J Biol Chem 287:26445-52. 2012
    ....
  33. pmc Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii
    Dimitri Tolleter
    Commissariat à l Énergie Atomique et aux Énergies Alternatives, Direction des Sciences du Vivant, Institut de Biologie Environementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, 13108 Saint Paul Lez Durance, France
    Plant Cell 23:2619-30. 2011
    ..Regulation of the trans-thylakoidal proton gradient by monitoring pgrl1 expression opens new perspectives toward reprogramming the cellular metabolism of microalgae for enhanced H₂ production...
  34. ncbi Proteomic approach to characterize the supramolecular organization of photosystems in higher plants
    Jesco Heinemeyer
    Institut für Angewandte Genetik, Universitat Hannover, Herrenhauser Str 2, D 30419 Hannover, Germany
    Phytochemistry 65:1683-92. 2004
    ..Further application of this experimental approach will allow new insights into the supermolecular organization of photosystems in plants...
  35. ncbi Crystal structure of plant photosystem I
    Adam Ben-Shem
    Department of Biochemistry, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Nature 426:630-5. 2003
    ....
  36. doi The long-term responses of the photosynthetic proton circuit to drought
    Kaori Kohzuma
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan
    Plant Cell Environ 32:209-19. 2009
    ....
  37. ncbi Digalactosyl-diacylglycerol deficiency impairs the capacity for photosynthetic intersystem electron transport and state transitions in Arabidopsis thaliana due to photosystem I acceptor-side limitations
    Alexander G Ivanov
    Department of Biology and The Biotron, University of Western Ontario, London, Ontario, Canada
    Plant Cell Physiol 47:1146-57. 2006
    ..These results are discussed in terms of thylakoid membrane domain reorganization in response to DGDG deficiency in A. thaliana...
  38. pmc The mutant of sll1961, which encodes a putative transcriptional regulator, has a defect in regulation of photosystem stoichiometry in the cyanobacterium Synechocystis sp. PCC 6803
    Tamaki Fujimori
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa shi, Chiba, Japan
    Plant Physiol 139:408-16. 2005
    ..Our results demonstrate that a transcriptional regulator, Sll1961, and its possible target proteins, including Sll1773, may be responsible for the regulation of photosystem stoichiometry in response to high light...
  39. ncbi Proteomic analysis of a highly active photosystem II preparation from the cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel polypeptides
    Yasuhiro Kashino
    Department of Biology, Washington University, One Brookings Drive, St Louis, MO 63130, USA
    Biochemistry 41:8004-12. 2002
    ..This work describes newly identified proteins in a highly purified cyanobacterial PSII preparation that is being widely used to investigate the structure, function, and biogenesis of this photosystem...
  40. ncbi A cyanobacterial gene family coding for single-helix proteins resembling part of the light-harvesting proteins from higher plants
    C Funk
    Department of Plant Biology, Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe 85287 1601, USA
    Biochemistry 38:9397-404. 1999
    ..The SCPs represent a new group of cyanobacterial proteins that, in view of their primary structure and response to deletion of photosystem I, are likely to be involved in transient pigment binding...
  41. ncbi N-terminal processing of Lhca3 Is a key step in remodeling of the photosystem I-light-harvesting complex under iron deficiency in Chlamydomonas reinhardtii
    Bianca Naumann
    Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 280:20431-41. 2005
    ..However, in contrast to iron deficiency, comparative MS/MS peptide profiling using isotopic labeling of proteins demonstrated that RNAi depletion of Lhca3 caused strong reduction of almost all Lhca proteins in isolated PSI particles...
  42. ncbi Irreversible damage to photosystem I by chilling in the light: cause of the degradation of chlorophyll after returning to normal growth temperature
    Hideki Kudoh
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Bldg FSB Box 101, Kashiwanoha 5 1 5, Kashiwa shi, Chiba 277 8562, Japan
    Planta 215:541-8. 2002
    ..e. irreversibility of the injury and development of visible symptoms at room temperature, can be explained as a consequence of the chilling-induced photoinhibition of PSI...
  43. pmc The water-water cycle as alternative photon and electron sinks
    K Asada
    Department of Biotechnology, Faculty of Engineering, Fukuyama University, Japan
    Philos Trans R Soc Lond B Biol Sci 355:1419-31. 2000
    ..The dual functions of the water-water cycle for protection from photoinhibition under photon excess stress are discussed, along with its functional evolution...
  44. ncbi Late assembly steps and dynamics of the cyanobacterial photosystem I
    Ulf Dühring
    Institute of Biology, Humboldt University Berlin, 10115 Berlin, Germany
    J Biol Chem 282:10915-21. 2007
    ..Implications of the presented data with respect to Ycf37 function are discussed...
  45. pmc The evolutionarily conserved tetratrico peptide repeat protein pale yellow green7 is required for photosystem I accumulation in Arabidopsis and copurifies with the complex
    Jana Stöckel
    Institut für Allgemeine Botanik und Pflanzenphysiologie, Friedrich Schiller University, 07747 Jena, Germany
    Plant Physiol 141:870-8. 2006
    ..Whereas the cyanobacterial protein mediates more efficient PSI accumulation, the higher plant protein is absolutely required for complex assembly or maintenance...
  46. pmc The chloroplast NAD(P)H dehydrogenase complex interacts with photosystem I in Arabidopsis
    Lianwei Peng
    Department of Botany, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    J Biol Chem 283:34873-9. 2008
    ..Although the NDH complex exists as a monomer in etioplasts, it interacts with PSI to form a supercomplex within 48 h during chloroplast development...
  47. doi Photosystem I gene cassettes are present in marine virus genomes
    Itai Sharon
    Faculty of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nature 461:258-62. 2009
    ....
  48. pmc Live-cell imaging of photosystem II antenna dissociation during state transitions
    Masakazu Iwai
    Institute of Low Temperature Science, Hokkaido University, N19 W8, Sapporo, Hokkaido 060 0819, Japan
    Proc Natl Acad Sci U S A 107:2337-42. 2010
    ..Possible implications of the unbound phospho-LHCII on energy dissipation are discussed...
  49. pmc Excess manganese differentially inhibits photosystem I versus II in Arabidopsis thaliana
    R Millaleo
    Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, Chile
    J Exp Bot 64:343-54. 2013
    ..The possible involvement mechanisms of Mn toxicity targeting specifically PSI are discussed...
  50. ncbi Photosystem I, an improved model of the stromal subunits PsaC, PsaD, and PsaE
    O Klukas
    Institut fur Kristallographie, Freie Universitat Berlin, Takustrassett 6, D 14195 Berlin, Germany
    J Biol Chem 274:7351-60. 1999
    ..The 11-transmembrane alpha-helices of these subunits can now be assigned uniquely to the hydrophobic segments identified by hydrophobicity analyses...
  51. ncbi Insertion of PsaK into the thylakoid membrane in a "Horseshoe" conformation occurs in the absence of signal recognition particle, nucleoside triphosphates, or functional albino3
    A Mant
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Biol Chem 276:36200-6. 2001
    ..The data raise the possibility of a wholly spontaneous insertion pathway...
  52. ncbi Enhancement of cyclic electron flow around PSI at high light and its contribution to the induction of non-photochemical quenching of chl fluorescence in intact leaves of tobacco plants
    Chikahiro Miyake
    Research institute of Innovative Technology for the Earth RITE, 9 2 Kizugawadai, Kizu cho, Soraku gun, Kyoto, 619 0292 Japan
    Plant Cell Physiol 45:1426-33. 2004
    ..These results indicate that limitation of LEF induced CEF-PSI, which, in turn, helped to dissipate excess photon energy by driving NPQ of Chl fluorescence...
  53. ncbi The photosystem I trimer of cyanobacteria: molecular organization, excitation dynamics and physiological significance
    N V Karapetyan
    A N Bakh Institute of Biochemistry, Russian Academy of Sciences, 117071, Moscow, Russia
    FEBS Lett 460:395-400. 1999
    ..Charge separation in the photosystem I reaction center is the fastest of all known reaction centers...
  54. doi Effect of PGR5 impairment on photosynthesis and growth in Arabidopsis thaliana
    Yuri Nakajima Munekage
    CEA Cadarache, DSV, IBEB, SBVME, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, UMR 6191 CNRS CEA Université Aix Marseille, F 13108 Saint Paul lez Durance, France
    Plant Cell Physiol 49:1688-98. 2008
    ..We discuss these data in relation to the role of PGR5-dependent regulatory mechanisms in tuning the ATP/NADPH ratio and preventing inactivation of PSI, especially under conditions of high irradiance or enhanced photorespiration...
  55. ncbi The monomeric photosystem I-complex of the diatom Phaeodactylum tricornutum binds specific fucoxanthin chlorophyll proteins (FCPs) as light-harvesting complexes
    Thomas Veith
    Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Siesmayerstrasse 70, D 60323 Frankfurt am Main, Germany
    Biochim Biophys Acta 1767:1428-35. 2007
    ..Blue Native-PAGE, gel filtration and first electron microscopy studies of the PSI-FCP sample revealed a monomeric complex comparable in size and shape to the PSI-LHCI complex of green algae...
  56. ncbi Isolation and structural characterization of trimeric cyanobacterial photosystem I complex with the help of recombinant antibody fragments
    G Tsiotis
    Max Planck Institut fur Biophysik, Frankfurt am Main, Germany
    Eur J Biochem 231:823-30. 1995
    ..The position of the bound Fv fragment on the trimeric PSI surface was determined by high-resolution electron microscopy and digital image processing...
  57. ncbi The assembly of protein subunits and cofactors in photosystem I
    Wolfram Saenger
    Institut für Chemie Kristallographie, Freie Universitat Berlin, Takustrasse 6, D 14195, Berlin, Germany
    Curr Opin Struct Biol 12:244-54. 2002
    ..Understanding the assembly of the 12 protein subunits and 128 cofactors in photosystem I allows us to study the possible functions of the individual players in this protein-cofactor complex...
  58. ncbi A dip in the chlorophyll fluorescence induction at 0.2-2 s in Trebouxia-possessing lichens reflects a fast reoxidation of photosystem I. A comparison with higher plants
    Petr Ilík
    Laboratory of Biophysics, Faculty of Science, Palacky University, tr Svobody 26, CZ 77146 Olomouc, Czech Republic
    Biochim Biophys Acta 1757:12-20. 2006
    ..The increasing part of the dip probably reflects a transient reduction of plastoquinone (PQ) pool that is not associated with cyclic electron flow around PSI. Possible causes of this MV-insensitive PQ reduction are discussed...
  59. pmc Efficient light harvesting in a dark, hot, acidic environment: the structure and function of PSI-LHCI from Galdieria sulphuraria
    Balakumar Thangaraj
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA
    Biophys J 100:135-43. 2011
    ..This tight coupling helps Galdieria perform efficient light harvesting under the low-light conditions present in its natural endolithic habitat...
  60. ncbi PsaK2 subunit in photosystem I is involved in state transition under high light condition in the cyanobacterium Synechocystis sp. PCC 6803
    Tamaki Fujimori
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Box 101, 5 1 5 Kashiwanoha, Kashiwa shi, Chiba 277 8562, Japan
    J Biol Chem 280:22191-7. 2005
    ..The results suggest that PsaK2-dependent state transition is essential for the growth of this cyanobacterium under high light condition...
  61. ncbi Excitation energy trapping in photosystem I complexes depleted in Lhca1 and Lhca4
    Janne A Ihalainen
    Vrije Universiteit Amsterdam, Department of Physics and Astronomy, Biophysics, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    FEBS Lett 579:4787-91. 2005
    ..We suggest that the specific location of each Lhca protein within the PSI-LHCI supercomplex is more important than the presence of so-called red chlorophylls in the Lhca proteins...
  62. ncbi Dynamic flexibility in the light reactions of photosynthesis governed by both electron and proton transfer reactions
    David M Kramer
    Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA
    Trends Plant Sci 9:349-57. 2004
    ....
  63. ncbi Regulation of photosystem I reaction center genes in Synechocystis sp. strain PCC 6803 during Light acclimation
    Mirkka Herranen
    Plant Physiology and Molecular Biology, Department of Biology, University of Turku, FIN 20014 Turku, Finland
    Plant Cell Physiol 46:1484-93. 2005
    ..The scantiness of PsaA translation in darkness despite the abundance of psaA transcripts demonstrated that the comprehensive regulation of PSI accumulation also involves regulation at the level of translation...
  64. pmc In vivo interactions between photosynthesis, mitorespiration, and chlororespiration in Chlamydomonas reinhardtii
    Laurent Cournac
    Département d Ecophysiologie Végétale et de Microbiologie, Laboratoire d Ecophysiologie de la Photosyntèse, UMR 163 CNRS CEA, Univ Meéditerranée CEA 1000, Saint Paul lez Durance, France
    Plant Physiol 129:1921-8. 2002
    ..We conclude that the plastid oxidase, similar to the mitochondrial alternative oxidase, becomes significantly engaged when the PQ pool becomes highly reduced, and thereby prevents its over-reduction...
  65. ncbi Chloroplast Cu/Zn-superoxide dismutase is a highly sensitive site in cucumber leaves chilled in the light
    Sun Mi Choi
    Department of Biology, Chungnam National University, Daejeon 305 764, Korea
    Planta 216:315-24. 2002
    ..From these results, we suggest that Cu/Zn-SOD is the primary target of the light-chilling stress, followed by subsequent inactivation of PSI by reactive oxygen species...
  66. pmc Phosphatidylglycerol is essential for oligomerization of photosystem I reaction center
    Ildiko Domonkos
    Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H 6701 Szeged, Hungary
    Plant Physiol 134:1471-8. 2004
    ..Our data suggest an indispensable role for PG in the PsaL-mediated assembly of the PSI reaction center...
  67. doi State transitions--the molecular remodeling of photosynthetic supercomplexes that controls energy flow in the chloroplast
    Jun Minagawa
    Nattional Institute for Basic Biology, Okazaki, Japan
    Biochim Biophys Acta 1807:897-905. 2011
    ..This article is part of a Special Issue entitled: Regulation of Electron Transport in Chloroplasts...
  68. ncbi Determining the limitations and regulation of photosynthetic energy transduction in leaves
    Neil R Baker
    Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, Essex, UK
    Plant Cell Environ 30:1107-25. 2007
    ..Non-invasive probing of electron fluxes through PSI and PSII, and proton fluxes across the thylakoid membranes can provide insights into the operation of such regulatory processes in vivo...
  69. doi Photosystem I complexes associated with fucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis
    Yohei Ikeda
    Graduate School of Life Science, University of Hyogo, Harima Science Garden City, Hyogo 678 1297, Japan
    Biochim Biophys Acta 1777:351-61. 2008
    ..The ratio of fucoxanthin to Chl a on the PS I-bound FCP was lower than that of weakly bound FCP, suggesting that PS I-bound FCP specifically functions as the mediator of energy transfer between weakly bound FCPs and the PS I RC...
  70. ncbi The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis
    H Oh-oka
    Department of Biology, Faculty of Science, Osaka University
    J Biochem 103:962-8. 1988
    ..The phylogenetic implications of the structure of the present polypeptide as compared with those of several bacterial-type ferredoxins are discussed...
  71. ncbi Effects of light intensity on cyclic electron flow around PSI and its relationship to non-photochemical quenching of Chl fluorescence in tobacco leaves
    Chikahiro Miyake
    Research institute of Innovative Technology for the Earth RITE, Kizu cho, Soraku gun, Kyoto, Japan
    Plant Cell Physiol 46:1819-30. 2005
    ..These results suggested that HL-plants dissipated the excess photon energy through NPQ by enhancing the ability of CEF-PSI to induce acidification of the thylakoid lumen...
  72. pmc Metabolic pathways for photobiological hydrogen production by nitrogenase- and hydrogenase-containing unicellular cyanobacteria Cyanothece
    Nicholas J Skizim
    Department of Chemistry and Chemical Biology, Waksman Institute of Microbiology, Rutgers University, Piscataway, New Jersey 08854, USA
    J Biol Chem 287:2777-86. 2012
    ..Comparison of photobiological hydrogen rates, yields, and energy conversion efficiencies reveals opportunities for improvement...
  73. ncbi Recombinant Lhca2 and Lhca3 subunits of the photosystem I antenna system
    Simona Castelletti
    Dipartimento Scientifico e Tecnologico, Universita di Verona, Strada Le Grazie, 15 37234 Verona, Italy
    Biochemistry 42:4226-34. 2003
    ..thaliana...
  74. doi Structure and function of intact photosystem 1 monomers from the cyanobacterium Thermosynechococcus elongatus
    Eithar El-Mohsnawy
    Plant Biochemistry, Ruhr University Bochum, 44780 Bochum, Germany
    Biochemistry 49:4740-51. 2010
    ....
  75. pmc Photosystem II complex in vivo is a monomer
    Takeshi Takahashi
    Graduate School of Life Science, University of Hyogo, 3 2 1 Kohto, Kamigohri, Ako gun, Hyogo 678 1297, Japan
    J Biol Chem 284:15598-606. 2009
    ..Our proposal will lead to a re-evaluation of many crystallographic models of membrane protein complexes in terms of their oligomerization status...
  76. pmc Quantification of cyclic and linear flows in plants
    Pierre Joliot
    Institut de Biologie PhysicoChimique, Centre National de la Recherche Scientifique, Unité Propre de Recherche 1261, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 102:4913-8. 2005
    ..A. (2001) Trends Plant Sci. 6, 349-354]...
  77. ncbi Plant photosystem I--the most efficient nano-photochemical machine
    Nathan Nelson
    Biochemistry Department, The George S Wise Faculty of Life Sciences, The Daniella Rich Institute for Structural Biology, Tel Aviv University, Tel Aviv 69978, Israel
    J Nanosci Nanotechnol 9:1709-13. 2009
    ..The structure should provide a template for designing artificial systems amenable for utilizable energy production...
  78. doi The long-term response to fluctuating light quality is an important and distinct light acclimation mechanism that supports survival of Arabidopsis thaliana under low light conditions
    Raik Wagner
    Junior Research Group, Institute for General Botany and Plant Physiology, Friedrich Schiller University Jena, Dornburger Str 159, 07743 Jena, Germany
    Planta 228:573-87. 2008
    ..Thus, the LTR represents a distinct acclimation response in addition to other already known responses that clearly improves plant physiology under low light conditions resulting in a pronounced positive effect on plant fitness...
  79. ncbi Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions
    Joanna Kargul
    Wolfson Laboratories, Division of Molecular Biosciences, Imperial College London, UK
    FEBS J 272:4797-806. 2005
    ....
  80. ncbi Structure and functional role of supercomplexes of IsiA and Photosystem I in cyanobacterial photosynthesis
    Roman Kouril
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    FEBS Lett 579:3253-7. 2005
    ..Work on mutants indicates that the PsaF/J and PsaL subunits facilitate the formation of closed rings around Photosystem I monomers but are not obligatory components in the formation of Photosystem I-IsiA supercomplexes...
  81. pmc Biochemical and structural studies of the large Ycf4-photosystem I assembly complex of the green alga Chlamydomonas reinhardtii
    Shin Ichiro Ozawa
    Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Plant Cell 21:2424-42. 2009
    ..A decrease in COP2 to 10% of wild-type levels by RNA interference increased the salt sensitivity of the Ycf4 complex stability but did not affect the accumulation of PSI, suggesting that COP2 is not essential for PSI assembly...
  82. ncbi Quenching of chlorophyll triplet states by carotenoids in reconstituted Lhca4 subunit of peripheral light-harvesting complex of photosystem I
    Donatella Carbonera
    Dipartimento di Scienze Chimiche, Universita di Padova, Via Marzolo 1, 35131 Padova, Italy
    Biochemistry 44:8337-46. 2005
    ..Lack of the red forms yields into a reduced efficiency of the triplet quenching in LHC-I thus suggesting that the "red Chls" play a role in enhancing triplet quenching in LHC-I and, possibly, in the whole photosystem I...
  83. ncbi The nature of a chlorophyll ligand in Lhca proteins determines the far red fluorescence emission typical of photosystem I
    Tomas Morosinotto
    Dipartimento Scientifico e Tecnologico, Universita di Verona, Strada Le Grazie, 15 37134 Verona, Italy
    J Biol Chem 278:49223-9. 2003
    ..Because the mutation does not affect Chl A5 orientation, we suggest that coordination by Asn of Chl A5 holds it at the correct distance with Chl B5...
  84. ncbi Molecular identification of a novel protein that regulates biogenesis of photosystem I, a membrane protein complex
    V V Bartsevich
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    J Biol Chem 272:6382-7. 1997
    ....
  85. ncbi A previously found thylakoid membrane protein of 14kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P
    Anastassia Khrouchtchova
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK 1871 Frederiksberg C, Copenhagen, Denmark
    FEBS Lett 579:4808-12. 2005
    ..The PSI-P protein was not differentially phosphorylated in state 1 and state 2...
  86. ncbi The structure of photosystem I and evolution of photosynthesis
    Nathan Nelson
    Department of Biochemistry, The George S Wise Faculty of Life Sciences, The Daniella Rich Institute for Structural Biology, Tel Aviv University, Israel
    Bioessays 27:914-22. 2005
    ..The available structural information complements the knowledge gained from genomic and proteomic data to illustrate a more precise scenario for the evolution of life systems on earth...
  87. ncbi A photosystem 1 psaFJ-null mutant of the cyanobacterium Synechocystis PCC 6803 expresses the isiAB operon under iron replete conditions
    Robert Jeanjean
    LCB CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    FEBS Lett 549:52-6. 2003
    ..Products are the IsiA chlorophyll-binding protein (CP43') and the isiB gene product flavodoxin. Supporting evidence was obtained by similar isiAB induction in wild type cells artificially exposed to oxidative stress...
  88. doi Plant photosystem I design in the light of evolution
    Alexey Amunts
    Biochemistry Department, George S Wise Faculty of Life Sciences, The Daniella Rich Institute for Structural Biology, Tel Aviv University, Tel Aviv, Israel
    Structure 17:637-50. 2009
    ..In addition, some aspects of PSI structure determination are discussed...
  89. pmc Proteomics of Chlamydomonas reinhardtii light-harvesting proteins
    Einar J Stauber
    Lehrstuhl fur Pflanzenphysiologie, Friedrich Schiller Universitat Jena, 07743 Jena, Germany
    Eukaryot Cell 2:978-94. 2003
    ..This proteomic study demonstrates the complexity of the light-harvesting proteins at the protein level in C. reinhardtii and will be an important basis of future functional studies addressing this diversity...
  90. ncbi Redox modulation of cyclic electron flow around photosystem I in C3 plants
    Cécile Breyton
    UMR 7099, CNRS Université Paris 7, Institut de Biologie Physico Chimique, Paris, France
    Biochemistry 45:13465-75. 2006
    ..This would be ultimately regulated by the balance between the redox state of PSI acceptors and donors during photosynthesis, in a diffusing system...
  91. pmc Comparison of the light-harvesting networks of plant and cyanobacterial photosystem I
    Melih K Sener
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 89:1630-42. 2005
    ..The finding suggests that after close packing of chlorophylls was achieved, constraints other than efficiency of the overall excitation transfer process precluded further evolution of pigment ordering...
  92. ncbi Isolation and characterization of photoautotrophic mutants of Chlamydomonas reinhardtii deficient in state transition
    M M Fleischmann
    Department of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland
    J Biol Chem 274:30987-94. 1999
    ..Mutant stt3-4b is partially deficient in state transition but is still able to phosphorylate LHCII. Potential factors affected in these mutant strains and the function of state transition in C. reinhardtii are discussed...
  93. ncbi Measurement of photochemical quenching of absorbed quanta in photosystem I of intact leaves using simultaneous measurements of absorbance changes at 830 nm and thermal dissipation
    Nikolai G Bukhov
    Groupe de Recherche en Energie et Information Biomoléculaires, Universite du Quebec a Trois Rivieres, C P 500 Trois Rivières, Quebec G9A 5H7, Canada
    Planta 216:630-8. 2003
    ..The above illustrates the strong photochemical energy quenching occurring when the reaction centers are in open state (reduced P700)...
  94. ncbi Control of energy dissipation and photochemical activity in photosystem I by NADP-dependent reversible conformational changes
    Subramanyam Rajagopal
    Groupe de Recherche en Energie et Information Biomoléculaires, Universite du Quebec a Trois Rivieres, C P 500 Trois Rivières, Quebec, Canada G9A 5H7
    Biochemistry 42:11839-45. 2003
    ..This new control mechanism of energy dissipation and photochemical activity by NADP(+)/NADPH is proposed to increase the turnover rate of PSI under conditions when both linear and cyclic electron transport activities must be supported...
  95. ncbi Three-dimensional reconstruction of a light-harvesting complex I-photosystem I (LHCI-PSI) supercomplex from the green alga Chlamydomonas reinhardtii. Insights into light harvesting for PSI
    Joanna Kargul
    Wolfson Laboratories, Department of Biological Sciences, South Kensington Campus, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 278:16135-41. 2003
    ..There was no evidence for oligomerization of Chlamydomonas PSI in contrast to the trimerization of PSI in cyanobacteria...
  96. ncbi Photosystem I is indispensable for photoautotrophic growth, CO2 fixation, and H2 photoproduction in Chlamydomonas reinhardtii
    K Redding
    Departments of Molecular Biology and Plant Biology, University of Geneva, 30 Quai Ernest Ansermet, CH1211 Geneva 4, Switzerland
    J Biol Chem 274:10466-73. 1999
    ..We conclude that the reported instances of CO2 fixation, H2 photoevolution, and photoautotrophic growth of photosystem I-deficient mutants result from the presence of unrecognized photosystem I...
  97. ncbi Supramolecular organization of thylakoid membrane proteins in green plants
    Jan P Dekker
    Faculty of Sciences, Division of Physics and Astronomy, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, Netherlands
    Biochim Biophys Acta 1706:12-39. 2005
    ....
  98. ncbi Stoichiometry of LHCI antenna polypeptides and characterization of gap and linker pigments in higher plants Photosystem I
    Matteo Ballottari
    Dipartimento Scientifico e Tecnologico, Universita di Verona, Verona, Italy
    Eur J Biochem 271:4659-65. 2004
    ....
  99. pmc Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time
    Patrick Kiley
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 3:e230. 2005
    ..These observations suggest that the peptide detergents may effectively stabilize membrane proteins in the solid-state. These designed peptide detergents may facilitate the study of diverse types of membrane proteins...
  100. ncbi A genome's-eye view of the light-harvesting polypeptides of Chlamydomonas reinhardtii
    D Elrad
    The Department of Plant Biology, The Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA
    Curr Genet 45:61-75. 2004
    ..reinhardtii genome. This is an important first step in designing specific genetic, biochemical, and physiological studies aimed at characterizing the composition, function, and regulation of the light-harvesting complexes...
  101. ncbi Lhca5--an LHC-type protein associated with photosystem I
    Ulrika Ganeteg
    Umea Plant Science Centre, Department of Plant Physiology, Umea University, S 901 87 Umea, Sweden
    Plant Mol Biol 54:641-51. 2004
    ..We propose that Lhca5 binds chlorophylls in a similar fashion to the other Lhca proteins and is associated with PSI only in sub-stoichiometric amounts...

Research Grants91

  1. FLAVIN-PROTEIN INTERACTIONS IN FLAVOENZYME CATALYSIS
    Gordon Tollin; Fiscal Year: 1993
    ..It is anticipated that these studies will provide new insights into the roles of protein structure in controlling molecular recognition, specificity and rates in biological electron transfer processes...
  2. STRUCTURE/FUNCTION OF PHOTOSYNTHETIC CYTOCHROME COMPLEX
    WILLIAM CRAMER; Fiscal Year: 1993
    ....
  3. Femtosecond nano-crystallography of membrane proteins
    Petra Fromme; Fiscal Year: 2010
    ..The pulses are so brief that they terminate before radiation damage processes can begin. ..
  4. DISTANCE MEASUREMENTS IN METALLOPROTEINS
    GARY BRUDVIG; Fiscal Year: 2002
    ....
  5. 250/500 GHZ GYROTRON BASED DNP/EPR SPECTROMETER
    RICHARD TEMKIN; Fiscal Year: 2003
    ..5-1.0% bandwidth) and advanced pulse formats for EPR studies. We will also extend gyrotron oscillators to 600-800 GHz. Such gyrotrons could be used with future 0.9- 1.2 GHz NMR spectrometers. ..
  6. SPECTROSCOPIC STUDIES OF LIGHT-DRIVEN ELECTRON TRANSFER
    BRIDGETTE BARRY; Fiscal Year: 2007
    ..abstract_text> ..
  7. PHYTOCHROME REGULATION OF SPECIFIC GENE EXPRESSION
    ELAINE TOBIN; Fiscal Year: 1991
    ..To isolate Arabidopsis mutants in the phytochrome response pathway(s) that govern nuclear transcriptional changes and light-dependent germination; and 8. To analyze the recovered mutants both genetically and biochemically...
  8. Disassembly and degradation of Photosystem I
    Kevin Redding; Fiscal Year: 2005
    ....
  9. Supercritical Fluid Extraction for Membrane Proteomics
    Lloyd Smith; Fiscal Year: 2006
    ..The successful development of this new technology for membrane proteomics will address a critical gap in current proteomics efforts. ..
  10. MOLECULAR AND CELLULAR BACTERIAL PATHOGENESIS
    David Briles; Fiscal Year: 2007
    ..The MCBP advisory committee will advise the trainees in their research and training programs and in their search for a job or postdoctoral position at the completion of their MCBP training. ..
  11. Mechanism of Action of Antibody to PspA
    David Briles; Fiscal Year: 2007
    ..Our analyses of the protection-eliciting and non-protection-eliciting epitopes on PspA should make it possible to design even better PspA vaccines in the future. ..
  12. Mechanism of Action of Antibody to PspA
    David Briles; Fiscal Year: 2003
    ..Our analyses of the protection-eliciting and non-protection-eliciting epitopes on PspA should make it possible to design even better PspA vaccines in the future. ..
  13. PROTECTIVE IMMUNITY TO PNEUMOCOCCAL SURFACE PROTEIN A
    David Briles; Fiscal Year: 1993
    ....
  14. PspA: A Potential Pneumococcal Vaccine Component
    David Briles; Fiscal Year: 2009
    ..pneumoniae is important because of the high complexity and cost of the polysaccharide-protein conjugate vaccine and the rapid evolution of pneumococci to evade the capsule-type-specific protection elicited by the conjugate vaccine. ..
  15. REGULATION OF BILIPROTEIN BIOSYNTHESIS IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 1993
    ..PCC 7409 in an attempt to generate at in vitro system for the analysis of transcriptional regulation in cyanobacteria. The studies proposed should greatly extend out knowledge of mechanisms regulating gene expression in photoautotrophs...
  16. New Methodology for Indole Alkaloid Syntheses
    Ken S Feldman; Fiscal Year: 2010
    ..The use of a new variant of the Pummerer reaction to control both oxidation level and reaction site within the indole nucleus forms the basis of this chemistry. ..
  17. Chemical and Biological Studies on Biaryl Phenolics
    Ken Feldman; Fiscal Year: 2005
    ..The search for selective 20S proteosome inhibitors among the Ntn-type proteases will be advanced by these investigations. ..
  18. ALKYNYLIODONIUM SALTS IN ORGANIC SYNTHESIS
    Ken Feldman; Fiscal Year: 2004
    ..Taken together, these studies will introduce new and concise techniques for preparing small polycyclic organic molecules of the type typically sought as pharmaceutical leads. ..
  19. New Methodology for Indole Alkaloid Syntheses
    Ken Feldman; Fiscal Year: 2007
    ..The use of a new variant of the Pummerer reaction to control both oxidation level and reaction site within the indole nucleus forms the basis of this chemistry. ..
  20. RADICAL MEDIATED CYCLIZATION REACTIONS
    Ken Feldman; Fiscal Year: 1993
    ..Successful completion of these syntheses will afford the targets (or analogs) in the most efficient manner to date...
  21. REGULATION OF BILIPROTEIN IN CYANOBACTERIA
    DONALD BRYANT; Fiscal Year: 2002
    ..These last studies could explain bloom disappearance in natural populations and could suggest new approaches for the control of problematic or toxic cyanobacteria in water supplies. ..