ecdysterone

Summary

Summary: A steroid hormone that regulates the processes of MOLTING or ecdysis in insects. Ecdysterone is the 20-hydroxylated ECDYSONE.

Top Publications

  1. pmc Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone
    Anna Petryk
    Department of Pediatrics, University of Minnesota, 516 Delaware Street SE, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:13773-8. 2003
  2. pmc Identification of 20-hydroxyecdysone late-response genes in the chitin biosynthesis pathway
    Qiong Yao
    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou, China
    PLoS ONE 5:e14058. 2010
  3. ncbi The Drosophila disembodied gene controls late embryonic morphogenesis and codes for a cytochrome P450 enzyme that regulates embryonic ecdysone levels
    V M Chavez
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Development 127:4115-26. 2000
  4. doi Physiological significance of alternatively spliced exon combinations of the single-copy gene class A chitin synthase in the insect Ostrinia furnacalis (Lepidoptera)
    M Qu
    School of Bioscience and Biotechnology, Dalian University of Technology, Dalian, China
    Insect Mol Biol 21:395-404. 2012
  5. ncbi Postembryonic hematopoiesis in Drosophila
    R Lanot
    UPR 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, Strasbourg Cedex, 67084, France
    Dev Biol 230:243-57. 2001
  6. ncbi Steroid control of longevity in Drosophila melanogaster
    Anne F Simon
    Division of Biology 156 29, California Institute of Technology, 1201 California Boulevard, Pasadena, CA 91125, USA
    Science 299:1407-10. 2003
  7. doi Developmental regulation of glycolysis by 20-hydroxyecdysone and juvenile hormone in fat body tissues of the silkworm, Bombyx mori
    Ling Tian
    Key Laboratory of Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    J Mol Cell Biol 2:255-63. 2010
  8. pmc Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body
    Ling Tian
    Key Laboratory of Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    BMC Genomics 11:549. 2010
  9. ncbi Ecdysone agonists: mechanism and importance in controlling insect pests of agriculture and forestry
    Arthur Retnakaran
    Great Lakes Forestry Center, Canadian Forest Service, Sault Ste Marie, Ontario, Canada
    Arch Insect Biochem Physiol 54:187-99. 2003
  10. ncbi Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster
    J Terashima
    Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    J Endocrinol 187:69-79. 2005

Research Grants

  1. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2007
  2. GENETIC CONTROL OF PROTEIN SYNTHESIS IN EUCARYOTES
    EDWARD BERGER; Fiscal Year: 1980
  3. THE ARRANGEMENT OF INFORMATION IN ANIMAL CHROMOSOMES
    DAVID HOGNESS; Fiscal Year: 1980
  4. GENETIC AND HORMONAL CONTROL OF NEURAL REORGANIZATION
    LINDA RESTIFO; Fiscal Year: 1990
  5. BIOSYNTHESIS OF RNA'S--MECHANISMS AND CONTROLS
    ELIZABETH KELLER; Fiscal Year: 1990
  6. MULTITARGET DRUGS: VERIFICATION-OF-PRINCIPLE STUDIES
    Alexander Varshavsky; Fiscal Year: 2000
  7. MULTITARGET DRUGS: VERIFICATION-OF-PRINCIPLE STUDIES
    Alexander Varshavsky; Fiscal Year: 2001
  8. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2006
  9. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2005
  10. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2004

Detail Information

Publications200 found, 100 shown here

  1. pmc Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone
    Anna Petryk
    Department of Pediatrics, University of Minnesota, 516 Delaware Street SE, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 100:13773-8. 2003
    ....
  2. pmc Identification of 20-hydroxyecdysone late-response genes in the chitin biosynthesis pathway
    Qiong Yao
    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou, China
    PLoS ONE 5:e14058. 2010
    ..However, ecdysone-responsive genes have not been previously characterized in the context of insect chitin biosynthesis...
  3. ncbi The Drosophila disembodied gene controls late embryonic morphogenesis and codes for a cytochrome P450 enzyme that regulates embryonic ecdysone levels
    V M Chavez
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Development 127:4115-26. 2000
    ....
  4. doi Physiological significance of alternatively spliced exon combinations of the single-copy gene class A chitin synthase in the insect Ostrinia furnacalis (Lepidoptera)
    M Qu
    School of Bioscience and Biotechnology, Dalian University of Technology, Dalian, China
    Insect Mol Biol 21:395-404. 2012
    ..Spliced exons 2a and 2b have also been observed in Bombyx mori; therefore, this work provides new insights into the regulation of insect chitin synthase, particularly in lepidopteran insects...
  5. ncbi Postembryonic hematopoiesis in Drosophila
    R Lanot
    UPR 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, Strasbourg Cedex, 67084, France
    Dev Biol 230:243-57. 2001
    ..Cells within all lymph gland lobes, including prohemocytes from posterior lobes, massively differentiate into a new cell type specifically devoted to encapsulation, the lamellocyte...
  6. ncbi Steroid control of longevity in Drosophila melanogaster
    Anne F Simon
    Division of Biology 156 29, California Institute of Technology, 1201 California Boulevard, Pasadena, CA 91125, USA
    Science 299:1407-10. 2003
    ..These observations demonstrate the importance of the ecdysone hormonal pathway, a new player in regulating longevity...
  7. doi Developmental regulation of glycolysis by 20-hydroxyecdysone and juvenile hormone in fat body tissues of the silkworm, Bombyx mori
    Ling Tian
    Key Laboratory of Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    J Mol Cell Biol 2:255-63. 2010
    ..Taken together, we conclude that 20E acts as a general blocker for glycolysis in the Bombyx fat body during molting and pupation, whereas the physiological role of JH is contrast with 20E during molting...
  8. pmc Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body
    Ling Tian
    Key Laboratory of Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    BMC Genomics 11:549. 2010
    ..Fat body produces humoral response molecules and hence is considered as the major organ involved in innate immunity...
  9. ncbi Ecdysone agonists: mechanism and importance in controlling insect pests of agriculture and forestry
    Arthur Retnakaran
    Great Lakes Forestry Center, Canadian Forest Service, Sault Ste Marie, Ontario, Canada
    Arch Insect Biochem Physiol 54:187-99. 2003
    ..Some insects, however, show resistance to these analogs and this could be, inter alia, due to an ATP Binding Cassette Transporter like system that selectively pumps out the analogs...
  10. ncbi Nutritional status affects 20-hydroxyecdysone concentration and progression of oogenesis in Drosophila melanogaster
    J Terashima
    Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    J Endocrinol 187:69-79. 2005
    ..Starvation causes the ovary to retain the ecdysteroid it produces, thus enabling individual egg chambers to undergo apoptosis and thus control the number of eggs produced in relation to food intake...
  11. ncbi Ecdysteroids during early embryonic development in silkworm Bombyx mori: metabolism and functions
    Haruyuki Sonobe
    Department of Biology, Faculty of Science and Engineering, Konan University, Kobe, Japan
    Zoolog Sci 21:503-16. 2004
    ..To explain the process of the increase in the level of 20E during embryonic development in B. mori eggs, a possible model is proposed...
  12. doi CYP18A1, a key enzyme of Drosophila steroid hormone inactivation, is essential for metamorphosis
    Emilie Guittard
    Equipe Biogenèse des Signaux hormonaux, Laboratoire Biologie du Développement, UMR7622 CNRS, UPMC, 7 quai St Bernard, F 75005 Paris, France
    Dev Biol 349:35-45. 2011
    ..Our data suggest that the inactivation of 20E is essential for proper development and that CYP18A1 is a key enzyme in this process...
  13. ncbi Agonists of an ecdysone-inducible mammalian expression system inhibit Fas Ligand- and TRAIL-induced apoptosis in the human colon carcinoma cell line RKO
    I Oehme
    Chemotherapeutisches Forschungsinstitut Georg Speyer Haus, Frankfurt, Germany
    Cell Death Differ 13:189-201. 2006
    ..Therefore, the ecdysone-inducible mammalian expression system may not be suitable for the analysis of apoptosis-related genes...
  14. ncbi 20-hydroxyecdysone upregulates apoptotic genes and induces apoptosis in the Bombyx fat body
    Ling Tian
    Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Arch Insect Biochem Physiol 79:207-19. 2012
    ..Taken together, we infer that 20E upregulates apoptotic genes and thus induces apoptosis in the Bombyx fat body during larval molting and the larval-pupal transition...
  15. ncbi Molecular evolution of the insect Halloween family of cytochrome P450s: phylogeny, gene organization and functional conservation
    Kim F Rewitz
    Department of Science, Systems and Models, Roskilde University, P O Box 260, 4000 Roskilde, Denmark
    Insect Biochem Mol Biol 37:741-53. 2007
    ..The results suggest that duplications of ancestral P450 genes that acquired novel functions may have been an important mechanism for evolving the ecdysteroidogenic pathway...
  16. doi An epidermis-specific chitin synthase CDNA in Choristoneura fumiferana: cloning, characterization, developmental and hormonal-regulated expression
    Dinakar R Ampasala
    Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, India
    Arch Insect Biochem Physiol 76:83-96. 2011
    ..These results implicate that CfCHS1 is involved in the chitin synthase and new chitin formation during molting in the insect...
  17. pmc The Drosophila nuclear receptors DHR3 and betaFTZ-F1 control overlapping developmental responses in late embryos
    Anne Françoise Ruaud
    Department of Human Genetics, University of Utah School of Medicine, 15 N 2030 E Room 2100, Salt Lake City, UT 84112 5330, USA
    Development 137:123-31. 2010
    ..Taken together, these studies define the lethal phenotypes of DHR3 and betaFTZ-F1 mutants, and provide evidence for functional bifurcation in the 20E-responsive transcriptional cascade...
  18. ncbi The steroid hormone 20-hydroxyecdysone enhances neurite growth of Drosophila mushroom body neurons isolated during metamorphosis
    R Kraft
    Arizona Research Laboratories, Division of Neurobiology, University of Arizona, Tucson, Arizona 85721 0077, USA
    J Neurosci 18:8886-99. 1998
    ..These results implicate 20E in enhancing axonal outgrowth of Kenyon cells to support MB remodeling during metamorphosis...
  19. pmc The steroid molting hormone Ecdysone regulates sleep in adult Drosophila melanogaster
    Hiroshi Ishimoto
    Department of Anesthesia, Carver College of Medicine, University of Iowa, 51 Newton Road, Iowa City, IA 52242, USA
    Genetics 185:269-81. 2010
    ....
  20. ncbi 20-Hydroxyecdysone increases fiber size in a muscle-specific fashion in rat
    Noemi Toth
    Department of Pharmacognosy, Szeged, Hungary
    Phytomedicine 15:691-8. 2008
    ..According to our results 20E may provide an alternative for substitution of anabolic-androgenic steroids in therapeutic treatments against muscle atrophy...
  21. ncbi Tissue-specific and stage-specific expression of two silkworm ecdysone receptor isoforms -- ecdysteroid-dependent transcription in cultured anterior silk glands
    M Kamimura
    National Institute of Sericultural and Entomological Science, Tsukuba, Japan
    Eur J Biochem 248:786-93. 1997
    ..These results suggest that the increase of BmEcR mRNAs during the fifth instar is induced in vivo by a small increase in ecdysteroids...
  22. ncbi [Effects of ecdysterone on the expression of NF-kappaB p65 in H2O2 induced oxidative damage of human lens epithelial cells]
    Chun yan Feng
    Department of Ophthalmology, Second People s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou
    Zhongguo Zhong Xi Yi Jie He Za Zhi 32:76-9. 2012
    To study the effects of ecdysterone (ECR) on the expression of nuclear factor (NF)-kappaB in H2O2 induced oxidative damage of human lens epithelial cells (HLECs).
  23. ncbi Beta-ecdysterone induces osteogenic differentiation in mouse mesenchymal stem cells and relieves osteoporosis
    Lei Gao
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, PR China
    Biol Pharm Bull 31:2245-9. 2008
    The effect on bone tissue of beta-ecdysterone, a type of ecdysteroid found in many plants, has not been previously investigated...
  24. pmc Ecdysteroid 7,9(11)-dien-6-ones as potential photoaffinity labels for ecdysteroid binding proteins
    Pauline C Bourne
    Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter, Devon, EX4 4PS, UK
    J Insect Sci 2:11. 2002
    ..Thus, ecdysteroid dienones show considerable potential as photoaffinity analogues for ecdysteroid binding proteins...
  25. doi An ecdysone receptor from the pentatomomorphan, Nezara viridula, shows similar affinities for moulting hormones makisterone A and 20-hydroxyecdysone
    Donya Tohidi-Esfahani
    CSIRO Molecular and Health Technologies, and CSIRO Food and Nutritional Sciences, Sydney Laboratory, PO Box 184, North Ryde, NSW 1670, Australia
    Insect Biochem Mol Biol 41:77-89. 2011
    ..8-7.5 nM), characteristic of ecdysone receptors. MakA has a similar affinity to 20E for both NvE10 and NvE11, consistent with MakA being a major moulting hormone in N. viridula...
  26. ncbi Inhibition of [3H]ponasterone A binding by ecdysone agonists in the intact Kc cell line
    Yoshiaki Nakagawa
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Insect Biochem Mol Biol 32:175-80. 2002
    ..The activity of tebufenozide analogs having an n-pentyl or n-hexyl group instead of a 4-ethylphenyl group was similar to that of RH-5849...
  27. pmc Induction of translatable mRNA for dopa decarboxylase in Drosophila: an early response to ecdysterone
    G P Kraminsky
    Proc Natl Acad Sci U S A 77:4175-9. 1980
    ..To obtain evidence that ecdysterone induces the appearance of this mRNA we made use of the temperature-sensitive ecd1 mutant. Garen et al...
  28. ncbi Critical role of desolvation in the binding of 20-hydroxyecdysone to the ecdysone receptor
    Christopher Browning
    Departement de Biologie et de Genomique Structurales, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard Sebastien Brant, Illkirch, France
    J Biol Chem 282:32924-34. 2007
    ..Together, the data demonstrate the critical role of ligand desolvation in determining binding affinity, with general implications for the binding of hormones to their cognate nuclear receptors...
  29. ncbi 20-Hydroxyecdysone indirectly regulates Hemolin gene expression in Hyalophora cecropia
    K Roxström-Lindquist
    Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden
    Insect Mol Biol 14:645-52. 2005
    ..Finally, by electrophoresis mobility shift assay we demonstrate that the HRE-IR forms specific complexes with nuclear extract proteins of normal pupae that increase after 20E stimulation...
  30. pmc Distinct roles of isoforms of the heme-liganded nuclear receptor E75, an insect ortholog of the vertebrate Rev-erb, in mosquito reproduction
    Josefa Cruz
    Department of Entomology and the Institute for Integrative Genome Biology, Riverside, CA 92521, USA
    Mol Cell Endocrinol 349:262-71. 2012
    ..Disruption of this signaling pathway could be explored in the design of mosquito control approaches...
  31. ncbi Effects of vitamin D3 and ecdysterone on free-radical lipid peroxidation
    A I Kuzmenko
    Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine
    Biochemistry (Mosc) 62:609-12. 1997
    ..and after peroral administration of low-molecular-weight biological steroids vitamin D3 (cholecalciferol) and ecdysterone. The kinetics of lipid chemiluminescence in model systems in vitro were determined in blood serum and ..
  32. pmc Genomic mapping of binding regions for the Ecdysone receptor protein complex
    Zareen Gauhar
    Institute for Genomics and Systems Biology, Departments of Human Genetics and Ecology and Evolution, The University of Chicago, Chicago, Illinois 60637, USA
    Genome Res 19:1006-13. 2009
    ..Initial mutational analysis of vrille in vivo reveals that it is required for metamorphosis...
  33. doi Protective effects of 20-hydroxyecdysone on CoCl(2)-induced cell injury in PC12 cells
    J Hu
    Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, People s Republic of China
    J Cell Biochem 111:1512-21. 2010
    ..Based on our results, 20-Hydroxyecdysone may be a potential candidate for intervention in hypoxic-ischemic brain injuries such as stroke...
  34. ncbi [Vitamin D3 and 20-hydroxyecdysone -- inhibitors of free radical lipid oxidation during D-hypervitaminosis]
    A I Kuz'menko
    Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv
    Ukr Biokhim Zh 73:44-50. 2001
    ..20-Hydroxyecdysone under these conditions have been revealed as inducing accumulation in the mitochondrial and microsomal membranes of the substances with lambda 225 nm...
  35. ncbi Antagonistic actions of ecdysone and insulins determine final size in Drosophila
    Julien Colombani
    CNRS University of Nice Sophia Antipolis, UMR6543, Parc Valrose, 06108 Nice Cedex 2, France
    Science 310:667-70. 2005
    ..Hence, ecdysone counteracts the growth-promoting action of insulins, thus forming a humoral regulatory loop that determines organismal size...
  36. ncbi Binding affinity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity
    Chieka Minakuchi
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Eur J Biochem 270:4095-104. 2003
    ....
  37. pmc Molecular determinants of differential ligand sensitivities of insect ecdysteroid receptors
    S F Wang
    Programs in Genetics, Cell and Molecular Biology, Department of Entomology, Michigan State University, East Lansing, Michigan 48824, USA
    Mol Cell Biol 20:3870-9. 2000
    ..These results demonstrated that ecdysone could function as a bona fide ligand in a species-specific manner...
  38. ncbi Role of caspases and mitochondria in the steroid-induced programmed cell death of a motoneuron during metamorphosis
    K L Hoffman
    Institute of Neuroscience, University of Oregon, Eugene, Oregon, 97403-1254, USA
    Dev Biol 229:517-36. 2001
    ....
  39. doi Hsc70 binds to ultraspiracle resulting in the upregulation of 20-hydroxyecdsone-responsive genes in Helicoverpa armigera
    Wei Wei Zheng
    School of Life Sciences, The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, Shandong University, Shanda Road 27, Jinan 250100, Shandong, China
    Mol Cell Endocrinol 315:282-91. 2010
    ..These data suggest that Hsc70 participates in the 20E signal transduction pathway via binding to USP1 and mediating the expression of EcRB1, USP1 and then a set of 20E-responsive genes...
  40. ncbi Steroid-triggered programmed cell death of a motoneuron is autophagic and involves structural changes in mitochondria
    Ginger Kinch
    Institute of Neuroscience, University of Oregon, Eugene, Oregon 97403 1254, USA
    J Comp Neurol 457:384-403. 2003
    ..This system permits the study of neuronal death in response to its bona fide developmental signal, the rise in a steroid hormone...
  41. ncbi An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes
    L L Restifo
    J Mol Biol 188:517-28. 1986
    We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 X 10(3) base-pairs...
  42. pmc A novel ecdysone receptor mediates steroid-regulated developmental events during the mid-third instar of Drosophila
    Benjamin F B Costantino
    School of Life Sciences, University of Nevada Las Vegas, Las Vegas, Nevada, United States of America
    PLoS Genet 4:e1000102. 2008
    ..This result is novel because it demonstrates for the first time that at least some 20E-mediated, mid-larval, developmental responses are controlled by an uncharacterized receptor that does not contain an RXR-like component...
  43. ncbi Phytoecdysteroids: biological aspects
    L Dinan
    Department of Biological Sciences, University of Exeter, Devon, UK
    Phytochemistry 57:325-39. 2001
    ..In the present review, the distribution, biosynthesis, biological significance and potential applications of phytoecdysteroids are summarised...
  44. ncbi Hormonal cross talk in insect development
    Edward B Dubrovsky
    Department of Biology, Dartmouth College, Hanover, NH 03755, USA
    Trends Endocrinol Metab 16:6-11. 2005
    ..Two transcription factors--BR-C and E75A--contribute to the cross-talk between the two hormones. It appears that BR-C is a key target of JH status quo action, and E75A is a part of the mechanism whereby JH prevents BR-C activation...
  45. pmc Ecdysterone regulatory elements function as both transcriptional activators and repressors
    L Dobens
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755
    Mol Cell Biol 11:1846-53. 1991
    A synthetic, 23-bp ecdysterone regulatory element (EcRE), derived from the upstream region of the Drosophila melanogaster hsp27 gene, was inserted adjacent to the herpes simplex virus thymidine kinase promoter fused to a bacterial gene ..
  46. ncbi PKC-mediated USP phosphorylation is required for 20E-induced gene expression in the salivary glands of Drosophila melanogaster
    Xiaoping Sun
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Arch Insect Biochem Physiol 62:116-27. 2006
    ..The composite data suggest that PKC-mediated USP phosphorylation is required for 20E-induced gene expression in the salivary glands of Drosophila melanogaster...
  47. pmc Distinct roles of Broad isoforms in regulation of the 20-hydroxyecdysone effector gene, Vitellogenin, in the mosquito Aedes aegypti
    Jinsong Zhu
    Department of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
    Mol Cell Endocrinol 267:97-105. 2007
    ..In particular, the isoform Z2 is required for 20E-mediated activation of Vg, while isoforms Z1 and Z4 serve as repressors to ensure appropriate termination of Vg expression...
  48. ncbi Steroid regulation of glue protein genes in Drosophila melanogaster
    L Hansson
    Hereditas 110:61-7. 1989
    ..This hormone-induced disappearance is, in contrast to the 68C transcripts, not inhibited by cycloheximide...
  49. ncbi Drosophila Sgs genes: stage and tissue specificity of hormone responsiveness
    M Lehmann
    Institut für Genetik Freie Universität Berlin, Germany
    Bioessays 18:47-54. 1996
    ..g. secretion enhancer binding proteins, which act together in a synergistic manner with the 20E receptor and early gene products to establish a stage- and tissue-specific expression pattern...
  50. pmc Direct repeats bind the EcR/USP receptor and mediate ecdysteroid responses in Drosophila melanogaster
    C Antoniewski
    Institut Jacques Monod, CNRS et Université Paris, France
    Mol Cell Biol 16:2977-86. 1996
    ..They also provide evidence that additional flanking regulatory sequences are crucially required to potentiate the hormonal response mediated by both types of elements and specify its spatial and temporal pattern...
  51. ncbi Ecdysone-regulated puff genes 2000
    C S Thummel
    Department of Human Genetics, Howard Hughes Medical Institute, University of Utah, Room 5100, 15 North 2030 East, Salt Lake City, UT 84112 5331, USA
    Insect Biochem Mol Biol 32:113-20. 2002
    ..This review surveys the recent characterization of these ecdysone-inducible genes and provides an overview of how they expand our understanding of ecdysone functions during development...
  52. pmc Anopheles gambiae males produce and transfer the vitellogenic steroid hormone 20-hydroxyecdysone to females during mating
    Emilie Pondeville
    FRE2852 Protéines, Biochimie Structurale et Fonctionnelle, Laboratoire Biogenèse des Stéroïdes, Universite Pierre et Marie Curie, 75252 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 105:19631-6. 2008
    ..This could thus lead to the identification of new target genes for manipulating male and/or female reproductive success, a promising way to reduce or eliminate mosquito population and therefore to control malaria transmission...
  53. ncbi Programmed cell death in the Drosophila CNS is ecdysone-regulated and coupled with a specific ecdysone receptor isoform
    S Robinow
    Department of Zoology, University of Washington, Seattle 98195
    Development 119:1251-9. 1993
    ..The correlation of a unique pattern of receptor isoform expression with a particular steroid-regulated fate suggests that variations in the pattern of receptor isoform expression may serve as important switches during development...
  54. ncbi Ligand specificity and developmental expression of RXR and ecdysone receptor in the migratory locust
    David C Hayward
    Molecular Genetics and Evolution Group and Centre for the Molecular Genetics of Development, Research School of Biological Sciences, Australian National University, P O Box 475, Canberra, ACT 2601, Australia
    J Insect Physiol 49:1135-44. 2003
    ..Neither LmRXR isoform, alone or in combination with LmEcR, bound JH III at nanomolar concentrations...
  55. pmc Ecdysone [corrected] receptor isoforms play distinct roles in controlling molting and metamorphosis in the red flour beetle, Tribolium castaneum
    Anjiang Tan
    Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY 40546, USA
    Mol Cell Endocrinol 291:42-9. 2008
    ..These data suggest that the EcR but not USP isoforms play distinct roles during the larval-pupal metamorphosis and EcRA plays a dominant role in transduction of ecdysteroid response in T. castaneum...
  56. ncbi 20E-regulated USP expression and phosphorylation in Drosophila melanogaster
    Q Song
    Department of Entomology, University of Missouri, 1 87 Agriculture Building, Columbia, MO 65211, USA
    Insect Biochem Mol Biol 33:1211-8. 2003
    ..Identification of the in vivo USP phosphorylation sites and signal transduction pathways that regulate the specific USP phosphorylation is currently underway...
  57. ncbi Molecular chaperones activate the Drosophila ecdysone receptor, an RXR heterodimer
    M N Arbeitman
    Department of Developmental Biology, Stanford University School of Medicine, California 94305, USA
    Cell 101:67-77. 2000
    ..We also show that two of the six (Hsp90 and Hsc70) are required in vivo for ecdysone receptor activity, and that EcR is the primary target of the chaperone complex...
  58. ncbi Downregulation of the tissue-specific transcription factor Fork head by Broad-Complex mediates a stage-specific hormone response
    N Renault
    Institut für Genetik der Freien Universität Berlin, Arnimallee 7, D 14195 Berlin, Germany
    Development 128:3729-37. 2001
    ..Furthermore, they suggest that the BR-C is placed at a different position within the 20E-controlled hierarchies than previously assumed, and that at least part of its pleiotropic functions are mediated by tissue-specific regulators...
  59. pmc Fork head controls the timing and tissue selectivity of steroid-induced developmental cell death
    Chike Cao
    Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA
    J Cell Biol 176:843-52. 2007
    ..More generally, they suggest that cell identity factors like Fkh play a pivotal role in the normal control of developmental cell death...
  60. ncbi The induction of DNA puff BhC4-1 gene is a late response to the increase in 20-hydroxyecdysone titers in last instar dipteran larvae
    L R Basso
    Departamento de Biologia Celular, Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Av Bandeirantes 3 900, Ribeirão Preto 14049 900, SP, Brazil
    Mech Dev 110:15-26. 2002
    ..In vitro studies using transgenic Drosophila indicate that both repressor and activator factors regulate the timing of BhC4-1 expression in salivary glands...
  61. pmc A new model for 20-hydroxyecdysone and dibenzoylhydrazine binding: a homology modeling and docking approach
    J M Wurtz
    Laboratoire de Biologie Structurale, Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, C U de Strasbourg, France
    Protein Sci 9:1073-84. 2000
    ..The homology model complexes provide new insights that can be exploited in the rational design of new environmentally safe insecticides...
  62. ncbi Dual control of midgut trehalase activity by 20-hydroxyecdysone and an inhibitory factor in the bamboo borer Omphisa fuscidentalis Hampson
    Nujira Tatun
    Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
    J Insect Physiol 54:351-7. 2008
    ..Taken together, at least two factors may participate in the change in midgut trehalase activity: increase in trehalase activity and decrease in trehalase inhibitor activity, both of which may be induced by 20E...
  63. pmc Identification and expression profile of a putative basement membrane protein gene in the midgut of Helicoverpa armigera
    Jia Lin Wang
    School of Life Sciences, Shandong University, Jinan, China
    BMC Dev Biol 7:76. 2007
    ..The midgut undergoes histolysis and remodeling during the larval to adult transition in holometabolous insects, but the molecular mechanisms underlying this process are not well understood...
  64. pmc The genomic response to 20-hydroxyecdysone at the onset of Drosophila metamorphosis
    Robert B Beckstead
    Department of Human Genetics, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT 84112 5331, USA
    Genome Biol 6:R99. 2005
    ..20E acts via a heterodimer of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspiracle, to directly regulate target gene transcription...
  65. ncbi Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster
    Yaning Sun
    Division of Plant Sciences, 1 31 Agriculture Building, University of Missouri, Columbia, Missouri 65211, USA
    J Proteome Res 6:4478-88. 2007
    ..The data further indicate that PKC-mediated phosphorylation is also required for genes regulated indirectly by 20E-induced changes in the larval salivary gland...
  66. pmc The effect of ecdysterone on cerebral vasospasm following experimental subarachnoid hemorrhage in vitro and in vivo
    Wei Hua Tang
    Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, China
    Neurol Res 30:571-80. 2008
    ..b>Ecdysterone is an insect steroid hormone...
  67. pmc Hormonal regulation of the humoral innate immune response in Drosophila melanogaster
    Thomas Flatt
    Division of Biology and Medicine, Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
    J Exp Biol 211:2712-24. 2008
    ..Our results suggest that 20E and JH play major roles in the regulation of gene expression in response to immune challenge...
  68. ncbi Hormonal regulation of mummy is needed for apical extracellular matrix formation and epithelial morphogenesis in Drosophila
    Anna Tonning
    Department of Medical Biochemistry, Goteborg University, Sweden
    Development 133:331-41. 2006
    ....
  69. doi The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm, Bombyx mori
    Daisuke Muramatsu
    Faculty of Agriculture and Life Sciences, Hirosaki University, Hirosaki 036 8561, Japan
    Mech Dev 125:411-20. 2008
    ....
  70. ncbi Hormonal control of a metamorphosis-specific transcriptional factor Broad-Complex in silkworm
    A M S Reza
    Developmental Biology Department, National Institute of Agrobiological Sciences, 1 2, Owashi, Tsukuba, Ibaraki 305 8634, Japan
    Comp Biochem Physiol B Biochem Mol Biol 139:753-61. 2004
    ..Thus, BR-C mRNAs are inducible by 20E only in the absence of JH, a finding that explains their metamorphosis-specific expression...
  71. ncbi Steroid regulated programmed cell death during Drosophila metamorphosis
    C Jiang
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah, Salt Lake City 84112, USA
    Development 124:4673-83. 1997
    ..These studies indicate that ecdysone directs the death of larval tissues via the precise stage- and tissue-specific regulation of key death effector genes...
  72. ncbi A trans-acting regulatory product necessary for expression of the Drosophila melanogaster 68C glue gene cluster
    T E Crowley
    Cell 39:149-56. 1984
    ..glue puff on the third chromosome does not regress when mutant salivary glands are cultured in the presence of ecdysterone; the three 68C glue protein mRNAs are not synthesized; and a transformed Drosophila strain carrying both a ..
  73. ncbi The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids
    M Schubiger
    Department of Zoology, University of Washington, Seattle WA 98195, USA
    Development 127:1151-9. 2000
    ..Our observations suggest that silencing by the unliganded EcR/USP receptor and the subsequent release of silencing by moderate steroid levels may play an important role in coordinating early phases of steroid driven development...
  74. doi Transcriptional regulation of the insulin signaling pathway genes by starvation and 20-hydroxyecdysone in the Bombyx fat body
    Yan Liu
    Key Laboratory of Insect Developmental Biology and Evolution, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    J Insect Physiol 56:1436-44. 2010
    ..We conclude that 20E slowly reduces food consumption and then indirectly induces a state of starvation resulting in the elevation of the mRNA levels of InR, IRS, PI3K110, and PDK in the Bombyx fat body during molting and pupation...
  75. ncbi Ecdysteroid control of cell proliferation during optic lobe neurogenesis in the moth Manduca sexta
    D T Champlin
    Department of Zoology, University of Washington, Seattle 98195 1800, USA
    Development 125:269-77. 1998
    ..Initially, moderate levels of ecdysteroid stimulate proliferation. Later, high levels of 20-hydroxyecdysone trigger a wave of apoptosis within the anlage that marks completion of its proliferative phase...
  76. ncbi Functional ecdysone receptor is the product of EcR and Ultraspiracle genes
    T P Yao
    Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, California 92037
    Nature 366:476-9. 1993
    ..These results redefine the ecdysone receptor as a dynamic complex whose activity may be altered by combinatorial interactions among subunits and ligand...
  77. ncbi Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle
    H E Thomas
    Gene Expression Programme, EMBL, Heidelberg, Germany
    Nature 362:471-5. 1993
    ..Whereas EcR-USP DNA-binding activity is unaffected by hormone, EcR-RXR DNA-binding activity is stimulated by either ecdysteroid or 9-cis-retinoic acid, demonstrating that hormone can play a role in heterodimer stabilization...
  78. ncbi Ecdysteroids govern two phases of eye development during metamorphosis of the moth, Manduca sexta
    D T Champlin
    Department of Zoology, University of Washington, Box 351800, Seattle, WA 98195 1800, USA
    Development 125:2009-18. 1998
    ..This capacity to regulate distinct phases of development by different concentrations of a single hormone is probably achieved by differential sensitivity of target gene promoters to induction by the hormone-bound receptor(s)...
  79. pmc The fork head product directly specifies the tissue-specific hormone responsiveness of the Drosophila Sgs-4 gene
    M Lehmann
    Institut für Genetik der Freien Universität Berlin, Germany
    EMBO J 15:4825-34. 1996
    ..Aside from being involved in the control of decisions during embryonic development, fkh thus participates directly in the control of specialized functions of differentiated cells at later stages of development...
  80. ncbi Glue secretion in the Drosophila salivary gland: a model for steroid-regulated exocytosis
    A Biyasheva
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Chicago, Illinois 60611 3093, USA
    Dev Biol 231:234-51. 2001
    ..The results presented establish a connection between 20E exposure and changes in Ca2+ levels that are mediated by Ca2+ effector proteins, and thus establish a mechanistic framework for future studies...
  81. ncbi Ecdysteroid-inducible genes in the programmed cell death during insect metamorphosis
    S Tsuzuki
    Division of Life Science, Graduate School of Natural Science and Technology, Kakumamachi, 920 1192, Kanazawa, Japan
    Insect Biochem Mol Biol 31:321-31. 2001
    ..Expression profiles of the genes after 20E stimulation indicated that four genes could be classified as early genes, while two are delayed early genes. The genes identified may provide insight into the PCD induced by a steroid hormone...
  82. ncbi Regulation of transcription factors MHR4 and betaFTZ-F1 by 20-hydroxyecdysone during a larval molt in the tobacco hornworm, Manduca sexta
    K Hiruma
    Department of Zoology, University of Washington, Seattle, Washington, 98195 1800, USA
    Dev Biol 232:265-74. 2001
    ..The presence of juvenile hormone had no effect on accumulation of either transcript...
  83. pmc Ecdysterone receptor is a sequence-specific transcription factor involved in the developmental regulation of heat shock genes
    Y Luo
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101
    Mol Cell Biol 11:3660-75. 1991
    Purification of ecdysterone receptor from Drosophila melanogaster to apparent homogeneity is reported...
  84. ncbi Steroid regulation of RNAs transcribed from the Drosophila 68c polytene chromosome puff
    T E Crowley
    Dev Biol 102:110-21. 1984
    ..The puff regresses near the end of the third instar as a result of an increased titer of the steroid hormone ecdysterone in the larval hemolymph...
  85. ncbi Broad-Complex, E74, and E75 early genes control DNA puff BhC4-1 expression in prepupal salivary glands
    L R Basso
    Departamento de Biologia Celular e Molecular e de Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Genesis 44:505-14. 2006
    ..Our results reveal that the BR-C Z3 isoform is essential for BhC4-1-lacZ induction in prepupal salivary glands and constitute the first demonstration of the participation of early genes products on DNA puff genes regulation...
  86. pmc Molecular analysis of the ecdysterone-inducible 2B5 "early' puff in Drosophila melanogaster
    A T Chao
    EMBO J 5:143-50. 1986
    The 2B5 region of the X-chromosome in Drosophila melanogaster plays a developmentally important role in the ecdysterone-triggered response of the late third instar salivary gland...
  87. ncbi The juvenile hormone analogue, methoprene, inhibits ecdysterone induction of small heat shock protein gene expression
    E M Berger
    Department of Biology, Dartmouth College, Hanover, New Hampshire 03755
    Dev Biol 151:410-8. 1992
    ..shock protein (hsp) genes of Drosophila are expressed in cultured cells in response to the moulting hormone, ecdysterone. We show here that juvenile hormone (JHIII) and the juvenile hormone analogue, methoprene, inhibit that ..
  88. ncbi Dense cluster of genes is located at the ecdysone-regulated 3C puff of Drosophila melanogaster
    M Furia
    Dipartimento di Genetica, Biologia Generale e Molecolare, Universita di Napoli, Italy
    J Mol Biol 231:531-8. 1993
    ..Interestingly to note, the entire cluster shows a peculiar genomic location, extending across two introns of the memory gene dunce, a large gene of Drosophila whose organization has proved to be remarkably complex...
  89. pmc A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm, Helicoverpa armigera
    Du Juan Dong
    The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education, School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China
    BMC Dev Biol 9:19. 2009
    ..The orthologs of eukaryotic initiation factor 5C (eIF5C) are essential to the initiation of protein translation, and their regulation during development is not well known...
  90. ncbi The steroid hormone-regulated gene Broad Complex is required for dendritic growth of motoneurons during metamorphosis of Drosophila
    Christos Consoulas
    ARL Division of Neurobiology, University of Arizona, Tucson, Arizona 85721, USA
    J Comp Neurol 485:321-37. 2005
    ..Candidates for mediating the effect of BRC-Z3 on dendritic growth of MN1-MN5 include their synaptic inputs and non-neuronal CNS cells that interact with them through direct contact or diffusible factors...
  91. ncbi Study of excretion of ecdysterone in human urine
    C Tsitsimpikou
    Doping Control Laboratory of Athens, Olympic Athletic Center of Athens, Kifissias 37, 15123 Maroussi, Greece
    Rapid Commun Mass Spectrom 15:1796-801. 2001
    A study of excretion in human urine of ecdysterone, which is the active component of several over-the-counter supplements such as "Ecdysten", reportedly used by athletes, is presented...
  92. ncbi Evidence for expression of EcR and USP components of the 20-hydroxyecdysone receptor by a mosquito cell line
    G Jayachandran
    Department of Entomology, University of Minnesota, St Paul, MN 55108, USA
    Arch Insect Biochem Physiol 43:87-96. 2000
    ..These transcripts appeared to be expressed constitutively and expression levels were not affected by treatment of cells with 20-hydroxyecdysone. Arch...
  93. ncbi Ecdysteroids during the third larval instar in 1(3)ecd-1ts, a temperature-sensitive mutant of Drosophila melanogaster
    P Berreur
    Gen Comp Endocrinol 54:76-84. 1984
    ..The mutant ecd-1 appears to present interesting opportunities for the detailed study of the hormonal induction of a developmental process during the third larval instar.(ABSTRACT TRUNCATED AT 250 WORDS)..
  94. ncbi Biological activities of a specific ecdysteroid dimer and of selected monomeric structural analogues in the B(II) bioassay
    Juraj Harmatha
    Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Flemingovo nam 2, 166 10 Prague, Czech Republic
    Insect Biochem Mol Biol 32:181-5. 2002
    ..The relatively high biological activity of this dimeric compound is discussed...
  95. ncbi Steroid regulation of midgut cell death during Drosophila development
    Cheng Yu Lee
    Center for Biosystems Research, University of Maryland Biotechnology Institute, University of Maryland, College Park, Maryland 20742, USA
    Dev Biol 250:101-11. 2002
    ..These studies indicate that ecdysone triggers a two-step hierarchy composed of steroid-induced regulatory genes and apoptosis genes that, in turn, regulate the autophagic death of midgut cells during development...
  96. ncbi Affinity labelling of a partially purified ecdysteroid receptor with a bromoacetylated 20-OH-ecdysone derivative
    M Strangmann-Diekmann
    Fakultat fur Chemie, Ruhr Universitat Bochum, Federal Republic of Germany
    Eur J Biochem 189:137-43. 1990
    ..The results also demonstrate that the ecdysteroid receptor of D. melanogaster has a molecular mass higher than all other vertebrate steroid hormone receptors studied so far...
  97. ncbi Differential control of MHR3 promoter activity by isoforms of the ecdysone receptor and inhibitory effects of E75A and MHR3
    Kiyoshi Hiruma
    Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
    Dev Biol 272:510-21. 2004
    ..In addition, E75A, another ecdysone-induced transcription factor, and MHR3 itself suppressed MHR3 promoter activity by binding to the monomeric response element (MRE2). Therefore, MHR3 can be down-regulated both by itself and by E75A...
  98. ncbi Genes that induce apoptosis: transcriptional regulation in identified, doomed neurons of the Drosophila CNS
    S Robinow
    Department of Zoology, University of Hawaii, 2538 The Mall, Honolulu, Hawaii 96822, USA
    Dev Biol 190:206-13. 1997
    ....
  99. doi Smt3 is required for Drosophila melanogaster metamorphosis
    Ana Talamillo
    Functional Genomics Unit, CIC bioGUNE, Technology Park, Building 801 A, 48160 Derio, Bizkaia, Spain
    Development 135:1659-68. 2008
    ..In summary, our study indicates that Smt3 is required for the ecdysteroid synthesis pathway at the time of puparium formation...
  100. ncbi Ecdysteroid control of metamorphosis in the differentiating adult leg structures of Drosophila melanogaster
    Christen Mirth
    Department of Biology, Box 351800, University of Washington, Seattle WA 98195, USA
    Dev Biol 278:163-74. 2005
    ..Lastly, leg structures had a finite ability to respond to 20E; tarsal joints lost competence to respond after 32-34 h APF, while the remaining structures became incompetent after 44-46 h APF...
  101. ncbi Tissue-specific regulation of juvenile hormone esterase gene expression by 20-hydroxyecdysone and juvenile hormone in Bombyx mori
    Manabu Kamimura
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
    Arch Insect Biochem Physiol 65:143-51. 2007
    ..JHE mRNA is thus expressed in tissue-specific manners under the control of ecdysteroids and JH...

Research Grants73

  1. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by the applicant): Ecdysterone and juvenile hormone (JH) are the primary endocrine regulators of reproduction and development in insects...
  2. GENETIC CONTROL OF PROTEIN SYNTHESIS IN EUCARYOTES
    EDWARD BERGER; Fiscal Year: 1980
    ..Finally, we are studying hormone effects in somatic cell hybrids between lines which differ in their pattern of response. ..
  3. THE ARRANGEMENT OF INFORMATION IN ANIMAL CHROMOSOMES
    DAVID HOGNESS; Fiscal Year: 1980
    ..At present, we have an in vitro system whereby the inhibitory activity found in extracts of heat-shocked cells can be assayed and wish to define the inhibitors as well as the enzymes of this RNA processing. ..
  4. GENETIC AND HORMONAL CONTROL OF NEURAL REORGANIZATION
    LINDA RESTIFO; Fiscal Year: 1990
    ..Thus, it is hypothesized that the steroid molting hormone ecdysterone orchestrates Drosophila CNS metamorphosis and that it does so primarily by modifying gene expression in the ..
  5. BIOSYNTHESIS OF RNA'S--MECHANISMS AND CONTROLS
    ELIZABETH KELLER; Fiscal Year: 1990
    ..We will look at gene expression in response to ecdysterone which stimulates cytoplasmic actin gene expression in this line...
  6. MULTITARGET DRUGS: VERIFICATION-OF-PRINCIPLE STUDIES
    Alexander Varshavsky; Fiscal Year: 2000
    ..cerevisiae that lack or express the corresponding macromolecular targets and their pairwise combinations. ..
  7. MULTITARGET DRUGS: VERIFICATION-OF-PRINCIPLE STUDIES
    Alexander Varshavsky; Fiscal Year: 2001
    ..cerevisiae that lack or express the corresponding macromolecular targets and their pairwise combinations. ..
  8. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by the applicant): Ecdysterone and juvenile hormone (JH) are the primary endocrine regulators of reproduction and development in insects...
  9. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2005
    DESCRIPTION (provided by the applicant): Ecdysterone and juvenile hormone (JH) are the primary endocrine regulators of reproduction and development in insects...
  10. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2004
    DESCRIPTION (provided by the applicant): Ecdysterone and juvenile hormone (JH) are the primary endocrine regulators of reproduction and development in insects...
  11. MOLECULAR & EVOLUTIONARY DETERMINANTS OF GENE REGULATION
    Douglas Cavener; Fiscal Year: 1990
    ..cis-acting regulatory elements responsible for the following properties of Gld expression will be determined: ecdysterone mediated induction, epidermal restriction, and adult male-limited expression...
  12. EUKARYOTIC GENES: STRUCTURE, FUNCTION, AND REGULATION
    GREGORY GUILD; Fiscal Year: 1980
    ..Several known and previously mapped mutations which apparently affect the expression and/or regulation of early and late gene sets will be investigated. ..
  13. GENE STRUCTURE AND FUNCTION IN ANIMAL DEVELOPMENT
    Elliot Meyerowitz; Fiscal Year: 1990
    ..One set will concentrate on the mechanisms by which two known trans-acting factors, the steroid hormone ecdysterone and the product of the X-chromosomeal gene l(1)npr-1, cause initiation of expression of the 68C genes...
  14. CONTROL OF BIOMINERALIZATION IN TWO SPECIES
    CHARLENE REED MILLER; Fiscal Year: 1980
    ..the effects on biomineralization of two factors, a homogenate from the visceral ganglion of the snails, and ecdysterone, will be examined...
  15. CLONING AND ANALYSIS OF THE MOSQUITO VITELLOGENIN GENE
    HENRY HAGEDORN; Fiscal Year: 1980
    ..several important questions: How is the expression of the vitellogenin gene regulated by juvenile hormone and ecdysterone? How many vitellogenin genes are there, and if multiple, are they coordinately expressed? How are the ..
  16. ANALYSIS OF DEVELOPMENT IN INSECTS
    Judith Willis; Fiscal Year: 1980
    ..The roles of ecdysterone and juvenile hormone in controlling the switch between pupal and adult synthesis in imaginal discs which are ..
  17. MECHANISM OF REGULATION OF HEAT SHOCK GENE EXPRESSION
    Richard Voellmy; Fiscal Year: 1991
    ..3) to purify and characterize another factor likely to be involved in the developmental regulation of the Drosophila hsp23 gene (ecdysterone receptor), and (4) to characterize a second type of developmental regulation of the hsp23 gene.
  18. MECHANISM OF REGULATION OF HEAT SHOCK GENE EXPRESSION
    Richard Voellmy; Fiscal Year: 1992
    ..3) to purify and characterize another factor likely to be involved in the developmental regulation of the Drosophila hsp23 gene (ecdysterone receptor), and (4) to characterize a second type of developmental regulation of the hsp23 gene.
  19. MECHANISM OF REGULATION OF HEAT SHOCK GENE EXPRESSION
    Richard Voellmy; Fiscal Year: 1990
    ..3) to purify and characterize another factor likely to be involved in the developmental regulation of the Drosophila hsp23 gene (ecdysterone receptor), and (4) to characterize a second type of developmental regulation of the hsp23 gene.
  20. Circadian control of a behavorial output
    John Ewer; Fiscal Year: 2006
    ..Thus it offers a unique opportunity to elucidate how the activity of the clock is coupled to the mechanism that controls a behavioral output. [unreadable] [unreadable] [unreadable]..
  21. Circadian control of a behavioral output
    John Ewer; Fiscal Year: 2007
    ..Thus it offers a unique opportunity to elucidate how the activity of the clock is coupled to the mechanism that controls a behavioral output. [unreadable] [unreadable] [unreadable]..
  22. Estrogen Receptor-related Receptor Action in Zebrafish
    Ann Tarrant; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  23. STEROID REGULATION OF PROGRAMMED CELL DEATH
    CARL THUMMEL; Fiscal Year: 2002
    ..In addition, this study will provide insight into the molecular mechanisms by which systemic hormonal signals are refined into stage- and tissue-specific biological responses during development. ..
  24. Regulation and Function of Drosophila Nuclear Receptors
    Carl S Thummel; Fiscal Year: 2010
    ....
  25. STEROID REGULATION OF PROGRAMMED CELL DEATH
    CARL THUMMEL; Fiscal Year: 2003
    ..In addition, this study will provide insight into the molecular mechanisms by which systemic hormonal signals are refined into stage- and tissue-specific biological responses during development. ..
  26. MOSQUITO IMMUNITY FUNCTIONS
    Ann Fallon; Fiscal Year: 2003
    ..The work will include identification of exon-intron organization and recovery of upstream flanking DNA, which will be analyzed for potential regulatory elements. ..
  27. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 2003
    ..These studies provide cloned genes, molecular approaches, and characterization of regulatory processes that can potentially be manipulated to interfere with disease transmission by blood-feeding arthropods. ..
  28. Mechanisms of Steroid-Triggered Programmed Cell Death
    CARL THUMMEL; Fiscal Year: 2009
    ..Finally, our studies provide a foundation for determining how steroids control cell death in humans, with implications for understanding and treating human disease. ..
  29. Induction of breast cancer in MMTVneu mice
    William Baldwin; Fiscal Year: 2002
    ..Overall, the purpose of this study is to examine 4-nonylphenol's effects on breast cancer growth and its mechanism of action on the mammary tumor. ..
  30. Transcriptional Control of Drosophila Detoxification Genes
    Carl S Thummel; Fiscal Year: 2010
    ..This research will provide new insights into how higher organisms, including humans, cope with toxic compounds in their environment and establish Drosophila as a new model system for characterizing xenobiotic response regulation. ..
  31. Optimizing Wolbachia manipulation in mosquito cell lines
    Ann Fallon; Fiscal Year: 2009
    ..We will optimize metabolic interactions between Wolbachia and mosquito host cells to maximize production and genetic manipulation of Wolbachia for control of mosquito-borne disease. ..
  32. Transcriptional Control of Drosophila Detoxification Genes
    CARL THUMMEL; Fiscal Year: 2009
    ..This research will provide new insights into how higher organisms, including humans, cope with toxic compounds in their environment and establish Drosophila as a new model system for characterizing xenobiotic response regulation. ..
  33. Mechanisms of Steroid-Triggered Programmed Cell Death
    CARL THUMMEL; Fiscal Year: 2008
    ..Finally, our studies provide a foundation for determining how steroids control cell death in humans, with implications for understanding and treating human disease. ..
  34. Regulation and Function of Drosophila Nuclear Receptors
    CARL THUMMEL; Fiscal Year: 2008
    ....
  35. Regulation and Function of Drosophila Nuclear Receptors
    CARL THUMMEL; Fiscal Year: 2009
    ....
  36. Regulation and Function of Drosophila Nuclear Receptors
    CARL THUMMEL; Fiscal Year: 2007
    ....
  37. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 2004
    ..These studies provide cloned genes, molecular approaches, and characterization of regulatory processes that can potentially be manipulated to interfere with disease transmission by blood-feeding arthropods. ..
  38. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 2002
    ..These studies provide cloned genes, molecular approaches, and characterization of regulatory processes that can potentially be manipulated to interfere with disease transmission by blood-feeding arthropods. ..
  39. MOSQUITO IMMUNITY FUNCTIONS
    Ann Fallon; Fiscal Year: 2002
    ..The work will include identification of exon-intron organization and recovery of upstream flanking DNA, which will be analyzed for potential regulatory elements. ..
  40. VASCULARIZATION IN TISSUE ENGINEERED CONSTRUCTS
    Charles Patrick; Fiscal Year: 2001
    ..does TEC-recipient inosculation play in TEC vascularization? (b) What are the biological functions of CD144, principally with regards to its role in inosculation and its regulation by the unique TEC microenvironment? ..
  41. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 1990
    ..These studies will contribute to an increased understanding of the genetics and physiology of the mosquito, a disease vector with considerable impact on human health and productivity...
  42. ECDYSONE EFFECTS ON MOSQUITO CELL PROLIFERATION
    Ann Fallon; Fiscal Year: 1999
    ..Results of this study will provide novel insights into the effects of 20E on cell growth and proliferation, which are directly relevant to efforts to control vector-borne disease by genetic manipulation of insect vectors. ..
  43. SYNTHGESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 1992
    ..These studies will contribute to an increased understanding of the genetics and physiology of the mosquito, a disease vector with considerable impact on human health and productivity...
  44. ECDYSONE EFFECTS ON MOSQUITO CELL PROLIFERATION
    Ann Fallon; Fiscal Year: 2000
    ..Results of this study will provide novel insights into the effects of 20E on cell growth and proliferation, which are directly relevant to efforts to control vector-borne disease by genetic manipulation of insect vectors. ..
  45. VASCULARIZATION IN TISSUE ENGINEERED CONSTRUCTS
    Charles Patrick; Fiscal Year: 2000
    ..does TEC-recipient inosculation play in TEC vascularization? (b) What are the biological functions of CD144, principally with regards to its role in inosculation and its regulation by the unique TEC microenvironment? ..
  46. MOSQUITO IMMUNITY FUNCTIONS
    Ann Fallon; Fiscal Year: 2001
    ..The work will include identification of exon-intron organization and recovery of upstream flanking DNA, which will be analyzed for potential regulatory elements. ..
  47. SYNTHESIS AND DEGRADATION OF RIBOSOMES IN THE MOSQUITO
    Ann Fallon; Fiscal Year: 1993
    ..These studies will contribute to an increased understanding of the genetics and physiology of the mosquito, a disease vector with considerable impact on human health and productivity...
  48. MOSQUITO IMMUNITY FUNCTIONS
    Ann Fallon; Fiscal Year: 2000
    ..The work will include identification of exon-intron organization and recovery of upstream flanking DNA, which will be analyzed for potential regulatory elements. ..
  49. Regulation and Function of Drosophila Nuclear Receptors
    CARL THUMMEL; Fiscal Year: 2009
    ....
  50. STEROID REGULATION OF PROGRAMMED CELL DEATH
    CARL THUMMEL; Fiscal Year: 2001
    ..In addition, this study will provide insight into the molecular mechanisms by which systemic hormonal signals are refined into stage- and tissue-specific biological responses during development. ..
  51. STEROID REGULATION OF PROGRAMMED CELL DEATH
    CARL THUMMEL; Fiscal Year: 2000
    ..In addition, this study will provide insight into the molecular mechanisms by which systemic hormonal signals are refined into stage- and tissue-specific biological responses during development. ..
  52. Transcriptional Control of Drosophila Detoxification Genes
    CARL THUMMEL; Fiscal Year: 2008
    ..This research will provide new insights into how higher organisms, including humans, cope with toxic compounds in their environment and establish Drosophila as a new model system for characterizing xenobiotic response regulation. ..
  53. VASCULARIZATION IN TISSUE ENGINEERED CONSTRUCTS
    Charles Patrick; Fiscal Year: 1999
    ..does TEC-recipient inosculation play in TEC vascularization? (b) What are the biological functions of CD144, principally with regards to its role in inosculation and its regulation by the unique TEC microenvironment? ..
  54. 3D Adipose Tissue Generation and Functional Verification
    Charles Patrick; Fiscal Year: 2004
    ..abstract_text> ..
  55. Mechanisms of Steroid-Triggered Programmed Cell Death
    CARL THUMMEL; Fiscal Year: 2007
    ..Finally, our studies provide a foundation for determining how steroids control cell death in humans, with implications for understanding and treating human disease. ..
  56. Mechanisms of Steroid-Triggered Programmed Cell Death
    CARL THUMMEL; Fiscal Year: 2005
    ..Finally, our studies provide a foundation for determining how steroids control cell death in humans, with implications for understanding and treating human disease. ..
  57. Mechanisms of Steroid-Triggered Programmed Cell Death
    CARL THUMMEL; Fiscal Year: 2006
    ..Finally, our studies provide a foundation for determining how steroids control cell death in humans, with implications for understanding and treating human disease. ..
  58. Regulation and Function of Drosophila Nuclear Receptors
    CARL THUMMEL; Fiscal Year: 2006
    ....