Genomes and Genes
Summary: An intermediate in the synthesis of cholesterol.
Publications152 found, 100 shown here
- Desmosterol can replace cholesterol in sustaining cell proliferation and regulating the SREBP pathway in a sterol-Delta24-reductase-deficient cell lineSara Rodríguez-Acebes
Hospital Ramon y Cajal, Madrid, Spain
Biochem J 420:305-15. 2009..This pathway is controlled by cholesterol and cholesterol-derived oxysterols. J774 cells cannot convert desmosterol into cholesterol, a defect resulting from the absence of mRNA for sterol-Delta24-reductase...
- Lipid metabolite profiling identifies desmosterol metabolism as a new antiviral target for hepatitis C virusMary A Rodgers
Department of Microbiology and Immunobiology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
J Am Chem Soc 134:6896-9. 2012..of the HCV JFH1 cell culture infection model, we identified a significant steady-state accumulation of desmosterol, an immediate precursor to cholesterol...
- Dose-dependent dual effects of cholesterol and desmosterol on J774 macrophage proliferationSara Rodríguez-Acebes
Servicio de Bioquímica Investigación, Hospital Ramon y Cajal, Carretera de Colmenar, Km 9, 28034 Madrid, Spain
Biochem Biophys Res Commun 377:484-8. 2008..ability of native, oxidized and acetylated low-density lipoproteins (nLDL, oxLDL and acLDL, respectively) and desmosterol to act as sources of sterol for the proliferation of J774A.1 macrophages. Treatment with 0...
- HPLC analysis of desmosterol, 7-dehydrocholesterol, and cholesterolE H Goh
Medical Sciences Program, Indiana University School of Medicine, Bloomington 47405
Lipids 24:652-5. 1989A simple and sensitive method to analyze mixtures of desmosterol, 7-dehydrocholesterol and cholesterol is described...
- Mutations in the 3beta-hydroxysterol Delta24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesisH R Waterham
Department of Pediatrics, Emma Children s Hospital, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
Am J Hum Genet 69:685-94. 2001..Patients with desmosterolosis have elevated levels of the cholesterol precursor desmosterol, in plasma, tissue, and cultured cells; this abnormality suggests a deficiency of the enzyme 3beta-..
- Uneven distribution of desmosterol and docosahexaenoic acid in the heads and tails of monkey spermW E Connor
Department of Medicine, Oregon Health Sciences University, Portland 97201, USA
J Lipid Res 39:1404-11. 1998Previously we demonstrated high concentrations of desmosterol and docosahexaenoic acid (DHA, 22:6 n-3) in monkey testes and sperm. Desmosterol, a cholesterol precursor, is not present elsewhere in the body...
- First synthesis of ent-desmosterol and its conversion to ent-deuterocholesterolEmily J Westover
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
Steroids 68:159-66. 2003We report the first synthesis of the unnatural enantiomer of desmosterol (ent-desmosterol). The sterol nucleus was constructed enantiospecifically, followed by stepwise addition of the side chain...
- Differential effects of cholesterol and desmosterol on the ligand binding function of the hippocampal serotonin(1A) receptor: implications in desmosterolosisPushpendra Singh
Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad 500 007, India
Biochim Biophys Acta 1788:2169-73. 2009..of cholesterol, we depleted cholesterol from native hippocampal membranes followed by replenishment with desmosterol. Desmosterol is an immediate biosynthetic precursor of cholesterol in the Bloch pathway differing only in a ..
- Biochemical markers for puberty in the monkey testis: desmosterol and docosahexaenoic acidW E Connor
Department of Medicine, Oregon Health Sciences University, Portland 97201, USA
J Clin Endocrinol Metab 82:1911-6. 1997We previously reported that the sperm of rhesus monkeys and humans uniquely contain large amounts of desmosterol not found in other tissues and have a high concentration of the highly polyunsaturated n-3 fatty acid, docosahexaenoic acid (..
- Sterol intermediates from cholesterol biosynthetic pathway as liver X receptor ligandsChendong Yang
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, 75390, USA
J Biol Chem 281:27816-26. 2006..the sterol was necessary and sufficient for activity, with the C-24 unsaturated cholesterol precursor sterols desmosterol and zymosterol exerting the largest effects...
- Cholesterol synthesis in the vertebrate retina: effects of U18666A on rat retinal structure, photoreceptor membrane assembly, and sterol metabolism and compositionS J Fliesler
Saint Louis University Eye Institute, Saint Louis University School of Medicine, Missouri 63104 1540
Lipids 35:289-96. 2000Treatment of neonatal rats with U18666A, an inhibitor of desmosterol delta24-reductase, results in accumulation of desmosterol (delta5,24) and depletion of cholesterol (delta5) in various bodily tissues and also causes cataracts...
- Studies on the cholesterol-free mouse: strong activation of LXR-regulated hepatic genes when replacing cholesterol with desmosterolMaura Heverin
Division of Clinical Chemistry, Karolinska University Hospital, Huddinge, S 14186, Sweden
Arterioscler Thromb Vasc Biol 27:2191-7. 2007Characterization of cholesterol homeostasis in male mice with a genetic inactivation of 3beta-hydroxysteroid-delta24-reductase, causing replacement of almost all cholesterol with desmosterol.
- Desmosterol may replace cholesterol in lipid membranesDaniel Huster
Junior Research Group Solid State NMR Studies of Membrane Associated Proteins, Biotechnological Biomedical Center, University of Leipzig, Germany
Biophys J 88:1838-44. 2005..For these animals, synthesis of cholesterol was interrupted at the level of its immediate precursor, desmosterol. Since cholesterol is a major and essential constituent of mammalian cellular membranes, we asked whether ..
- Significance of sterol structural specificity. Desmosterol cannot replace cholesterol in lipid raftsSaara Vainio
Institute of Biomedicine Anatomy, University of Helsinki, Helsinki FI 00014, Finland
J Biol Chem 281:348-55. 2006b>Desmosterol is an immediate precursor of cholesterol in the Bloch pathway of sterol synthesis and an abundant membrane lipid in specific cell types...
- Comparison of cholesterol and its direct precursors along the biosynthetic pathway: effects of cholesterol, desmosterol and 7-dehydrocholesterol on saturated and unsaturated lipid bilayersTomasz Rog
Department of Physics, Helsinki University of Technology, Otakaari 1, F1 02150 Espoo, Finland
J Chem Phys 129:154508. 2008..Here we elucidate this issue through atomic-scale simulations of desmosterol and 7-dehydrocholesterol (7DHC), which are immediate precursors of cholesterol in its two distinct biosynthetic ..
- Unique lipids of primate spermatozoa: desmosterol and docosahexaenoic acidD S Lin
Division of Endocrinology, Metabolism, and Clinical Nutrition, Oregon Health Sciences University, Portland 97201 3098
J Lipid Res 34:491-9. 1993..Two sterols were identified: cholesterol, 41%, and desmosterol, 59% of total sterols. Desmosterol was found only in the free form...
- Age-dependent increase in desmosterol restores DRM formation and membrane-related functions in cholesterol-free DHCR24-/- miceKatrin Kuehnle
Division of Psychiatry Research, University of Zurich, Zurich, Switzerland
Neurochem Res 34:1167-82. 2009..DHCR24-knock-out mice lack cholesterol and accumulate desmosterol with age...
- Generation of viable cholesterol-free miceA Wechsler
Quark Biotech, Inc, 10265 Carnegie Avenue, Cleveland, OH 44106, USA
Science 302:2087. 2003
- Role of sterol type on lateral pressure profiles of lipid membranes affecting membrane protein functionality: Comparison between cholesterol, desmosterol, 7-dehydrocholesterol and ketosterolO H Samuli Ollila
Institute of Physics, Tampere University of Technology, P O Box 692, FI 33101 Tampere, Finland
J Struct Biol 159:311-23. 2007..We find that the lateral pressure profile is altered when cholesterol is replaced by either desmosterol, 7-dehydrocholesterol, or ketosterol...
- Desmosterolosis presenting with multiple congenital anomalies and profound developmental delayHans C Andersson
Hayward Genetics Center and Department of Pediatrics, Tulane University Medical School, New Orleans, Louisiana 70112, USA
Am J Med Genet 113:315-9. 2002b>Desmosterol (cholesta-5,24-dien-3beta-ol) is a minor sterol that forms as an intermediate in the cholesterol biosynthetic pathway when the 24-unsaturated sterol bond is reduced as the last step rather than earlier in the conversion of ..
- ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brainNan Wang
Department of Medicine, Columbia University, PS 8 401, 630 W 168th St, New York, NY 10032, USA
FASEB J 22:1073-82. 2008..mice, levels of several sterol intermediates in the cholesterol biosynthetic pathway, namely desmosterol, lathosterol, and lanosterol, as well as 27-OH cholesterol, were increased 2- to 3-fold...
- Cholesterol substitution increases the structural heterogeneity of caveolaeMaurice Jansen
Institute of Biomedicine Anatomy, University of Helsinki, Helsinki, Finland
J Biol Chem 283:14610-8. 2008..the structure and function of caveolae, we replaced cholesterol in mammalian cells with its immediate precursor desmosterol by inhibiting 24-dehydrocholesterol reductase...
- Identification of a sterol Delta7 reductase gene involved in desmosterol biosynthesis in Mortierella alpina 1S-4Shuo Zhang
Division of Applied Life of Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo ku, Kyoto 606 8502, Japan
Appl Environ Microbiol 73:1736-41. 2007..Delta7 reductase from the filamentous fungus Mortierella alpina 1S-4, which accumulates cholesta-5,24-dienol (desmosterol) as the main sterol, revealed that the open reading frame of this gene, designated MoDelta7SR, consists of 1,..
- Homology modelling of human DHCR24 (seladin-1) and analysis of its binding properties through molecular docking and dynamics simulationsAlessandro Pedretti
Istituto di Chimica Farmaceutica e Tossicologica Pietro Pratesi, Facolta di Farmacia, Universita degli Studi di Milano, Via Mangiagalli 25, I 20133 Milano, Italy
Steroids 73:708-19. 2008..of the enzyme was obtained based on plant Cytokinin Dehydrogenase, and its active site was found to bind the desmosterol plus a set of post-squalenic intermediates of the cholesterol biosynthesis in a binding mode conducive to ..
- Limb malformations of rat fetuses exposed to a distal inhibitor of cholesterol biosynthesisFrançoise Chevy
INSERM Unité 538, CHU Saint Antoine, 27 rue Chaligny, Paris 75012, France
J Lipid Res 43:1192-200. 2002Triparanol, an inhibitor of desmosterol Delta24 reductase, produces a high rate of limb malformations in rat fetuses exposed at gestational day 10 (gd 10) to a single oral dose (150-200 mg/kg) given to the pregnant dam...
- [Synthesis of C24-functionalized hydroxysterols]V A Khripach
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, ul Kuprevicha 5 2, Minsk, 220141 Belarus
Bioorg Khim 28:284-8. 2002..The asymmetric hydroxylation of desmosterol acetate according to Sharpless was used as the key reaction in the stereoselective introduction of ..
- Interaction between cholesterol and glucose metabolism during dietary carbohydrate modification in subjects with the metabolic syndromeMaarit Hallikainen
Department of Clinical Nutrition, University of Kuopio, Kuopio, Finland
Am J Clin Nutr 84:1385-92. 2006..Carbohydrate modification based on rye bread and pasta enhances early insulin secretion in subjects with the metabolic syndrome...
- HPLC method for quantification and characterization of cholesterol and its oxidation products in eggsMônica R Mazalli
Department of Food Science, Faculty of Food Engineering, State University of Campinas, 13083 862 Campinas, SP, Brazil
Lipids 41:615-22. 2006..The method showed accuracy and precision with a detection limit between 0.002 and 0.079 microg/g. The oxides cholest-5-ene-3beta,20alpha-diol and cholest-5-ene-3beta,25-diol identified by HPLC-UV-RI were not confirmed by HPLC-APCI-MS...
- Impact of simvastatin, niacin, and/or antioxidants on cholesterol metabolism in CAD patients with low HDLNirupa R Matthan
Cardiovascular Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
J Lipid Res 44:800-6. 2003..Treatment with S-N reduced desmosterol and lathosterol levels (cholesterol synthesis indicators) 46% and 36% (P < 0...
- Analysis of sterols by high-performance liquid chromatography/mass spectrometry combined with chemometricsKornél Nagy
Department of Mass Spectrometry, Institute of Structural Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri ut 59 67, H 1025 Budapest, Hungary
Rapid Commun Mass Spectrom 20:2433-40. 2006..plasma concentrations of various phytosterols (cholestanol and beta-sitosterol) and cholesterol metabolites (desmosterol and lathosterol). This was based on an unusual solvent combination of water/methanol vs...
- Postprandial behavior of plasma squalene and non-cholesterol sterols in men with varying cholesterol absorptionM Hallikainen
Department of Clinical Nutrition, University of Kuopio, P O Box 1627, FIN 70211, Kuopio, Finland
Clin Chim Acta 374:63-8. 2006..lipid clearance (squalene as the surrogate marker) and postprandial cholesterol metabolism evaluated with plasma levels of cholesterol absorption (cholestanol and plant sterols) and synthesis markers (desmosterol and lathosterol).
- Oxysterols suppress inducible nitric oxide synthase expression in lipopolysaccharide-stimulated astrocytes through liver X receptorChang Seok Lee
Department of Pharmacology, Ajou University School of Medicine, Suwon, Korea
Neuroreport 17:183-7. 2006..Thus, we conclude that it is most likely mediated by liver X receptor/retinoid X receptor heterodimers...
- DHCR24 gene knockout mice demonstrate lethal dermopathy with differentiation and maturation defects in the epidermisRusella Mirza
Department of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya University, Chikusa ku, Nagoya, Japan
J Invest Dermatol 126:638-47. 2006..in the 3beta-hydroxysterol-Delta24 reductase (DHCR24) gene that encodes an enzyme catalyzing the conversion of desmosterol to cholesterol...
- A comparison of the behavior of cholesterol and selected derivatives in mixed sterol-phospholipid Langmuir monolayers: a fluorescence microscopy studyErin E Berring
Department of Chemistry, Saint Louis University, Monsanto Hall 125, 3501 Laclede Avenue, St Louis, MO 63103 2010, USA
Chem Phys Lipids 136:1-12. 2005..In this study, the interactions of CHOL and the related sterols desmosterol (DES) and 7DHC with l-alpha-dipalmitoylphosphatidylcholine (DPPC) monolayers were compared...
- The distribution of squalene and non-cholesterol sterols in lipoproteins in type 2 diabetesPiia P Simonen
Department of Medicine, Division of Internal Medicine, University of Helsinki, Biomedicum Helsinki, C4 22, P O Box 700, FIN 00029 HUS, Helsinki, Finland
Atherosclerosis 194:222-9. 2007..Lipoprotein distribution of non-cholesterol sterols was studied to evaluate in which lipoproteins they are carried in type 2 diabetes with body weight ranging from normal to overweight...
- Alterations of cholesterol synthesis precursors (7-dehydrocholesterol, 7-dehydrodesmosterol, desmosterol) in dysmyelinating neurological mutant mouse (quaking, shiverer and trembler) in the PNS and the CNSJ M Bourre
INSERM Unite 26, Hopital Fernand Widal, Paris, France
Biochim Biophys Acta 1004:387-90. 1989In brain, levels of cholesterol, desmosterol and 7-dehydrodesmosterol are reduced in shiverer and quaking, but not in trembler 60-day-old dysmyelinating mutant mice. Very interestingly, 7-dehydrocholesterol is not altered in any mutant...
- On the role of liver X receptors in lipid accumulation in adipocytesLene K Juvet
Institute for Nutrition Research, University of Oslo, N 0316 Oslo, Norway
Mol Endocrinol 17:172-82. 2003..These findings imply a role for LXR in controlling lipid storage capacity in mature adipocytes and point to an intriguing physiological interplay between LXR and PPARgamma in controlling pathways in lipid handling...
- Fatty acid and sterol composition of frozen and freeze-dried New Zealand Green Lipped Mussel (Perna canaliculus) from three sites in New ZealandKaren J Murphy
Department of Food Science, RMIT University, Melbourne, Australia
Asia Pac J Clin Nutr 12:50-60. 2003..Cholesterol was the most prominent sterol (31% of total sterols). Other major sterols included desmosterol/brassicasterol (co-eluting), 24-methylenecholesterol, trans-22-dehydrocholesterol, 24-nordehydrocholesterol and ..
- Effects of ezetimibe, simvastatin, atorvastatin, and ezetimibe-statin therapies on non-cholesterol sterols in patients with primary hypercholesterolemiaGerd Assmann
Leibniz Institute of Arteriosclerosis Research at the University of Münster, and Institute for Clinical Chemistry and Laboratory Medicine, University Clinics Münster, Munster, Germany
Curr Med Res Opin 24:249-59. 2008..g., desmosterol, lathosterol) and their ratios to TC correlate with cholesterol biosynthesis...
- [Factors modulating parameters of cholesterol homeostasis in the metabolic syndrome]A Zak
IV interní klinika 1, LF UK a VFN, Praha
Cas Lek Cesk 146:357-66. 2007..The concentrations of cholesterol precursors (lathosterol, desmosterol, respectively their rations to cholesterol) are related to the synthesis of cholesterol; concentrations of ..
- Effect of chemical carcinogens on cholesterol biosynthetic pathways in the skin of miceY Tanimoto
Department of Oral and Maxillofacial Surgery I, Hiroshima University, School of Dentistry, Japan
Carcinogenesis 11:1647-51. 1990..mouse skin only lathosterol was a major metabolite and the other metabolites, cholesta-5,7,24-trien-3 beta-ol, desmosterol, cholesterol and 7-dehydrocholesterol were much less abundant...
- Quantitative analysis of five sterols in amniotic fluid by GC-MS: application to the diagnosis of cholesterol biosynthesis defectsC Amaral
Departamento de Quimica, Universidade da Beira Interior, Rua Marques d Avila e Bolama, 6200 001 Covilhã, Portugal
J Chromatogr B Analyt Technol Biomed Life Sci 878:2130-6. 2010Cholesterol and its precursors, namely 7-dehydrocholesterol, desmosterol and lathosterol are important biochemical markers of cholesterol biosynthesis, and their quantification in body fluids is useful for the diagnosis of cholesterol ..
- Twenty-one year tracking of serum non-cholesterol sterols. The Cardiovascular Risk in Young Finns studyT A Miettinen
Department of Medicine, Division of Internal Medicine, University of Helsinki, Biomedicum Helsinki, room C4 22, P O Box 700, FIN 00029 HUS, Finland
Nutr Metab Cardiovasc Dis 19:525-31. 2009To show tracking of cholesterol metabolism, the ratios to cholesterol of e.g. serum cholestenol, desmosterol, and lathosterol, reflecting cholesterol synthesis, and cholestanol, campesterol, avenasterol and sitosterol, reflecting ..
- Synthesis and absorption markers of cholesterol in serum and lipoproteins during a large dose of statin treatmentT A Miettinen
University of Helsinki, Helsinki, Finland
Eur J Clin Invest 33:976-82. 2003..Serum contains noncholesterol sterols, which are reliable markers of cholesterol metabolism, but their presence and importance in different lipoproteins have been insufficiently studied...
- Seasonal and geographical variations of sterol composition in snow crab hepatopancreas and pelagic fish viscera from Eastern QuebecNathalie Souchet
Departement de Biologie, chimie et géographie, Universite du Quebec a Rimouski, Rimouski, Quebec, Canada
Comp Biochem Physiol B Biochem Mol Biol 147:378-86. 2007..25 mg/100 g wet basis for cholesterol and desmosterol; 0.03-0.05 mg/100 g for other sterols), good reproducibility (CV%: 1.5-6.8) and accuracy (recovery%: 94-107)...
- Progestins block cholesterol synthesis to produce meiosis-activating sterolsB Lindenthal
Department of Physiology and Experimental Medicine, The George Washington University School of Medical and Health Sciences, Washington, DC 20037, USA
FASEB J 15:775-84. 2001..were potent inhibitors of Delta24-reduction in an in vitro cell assay and led to the accumulation of desmosterol, a Delta5,24 sterol precursor of cholesterol...
- Effects of calcium and plant sterols on serum lipids in obese Zucker rats on a low-fat dietTimo Vaskonen
Institute of Biomedicine, Pharmacology, University of Helsinki, Finland
Br J Nutr 87:239-45. 2002..913, P<0.001, n 18), whereas serum desmosterol: cholesterol and lathosterol: cholesterol indicated that Ca dose-dependently increased endogenous cholesterol ..
- Cytochrome P450 17alpha hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cellsYing Liu
Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, 3900 Reservoir Road Northwest, Washington, D C 20057, USA
Mol Endocrinol 19:1918-31. 2005..substrates indicated that unlike squalene, addition of squalene epoxide, lanosterol, zymosterol, and desmosterol could rescue the hormone-induced progesterone formation...
- Phytosterol biosynthesis pathway in Mortierella alpinaW David Nes
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409 1061, USA
Phytochemistry 67:1716-21. 2006..The principal sterol of the mycelium was cholesta-5, 24-dienol (desmosterol) (83%), with lesser amounts of 24beta-methyl-cholesta-5,25(27)-dienol (codisterol) (2%), 24-methyldesmosterol (..
- Interannual and between species comparison of the lipids, fatty acids and sterols of Antarctic krill from the US AMLR Elephant Island survey areaCharles F Phleger
Department of Biology, San Diego State University, San Diego, CA 92182, USA
Comp Biochem Physiol B Biochem Mol Biol 131:733-47. 2002..tricantha. Cholesterol was the major sterol (80-100% of total sterols) with desmosterol second in abundance (1-18%). 1997 T. macrura and E...
- Expression of the novel adrenocorticotropin-responsive gene selective Alzheimer's disease indicator-1 in the normal adrenal cortex and in adrenocortical adenomas and carcinomasPaola Luciani
Endocrine Unit, Department of Clinical Physiopathology, University of Florence, 50139 Florence, Italy
J Clin Endocrinol Metab 89:1332-9. 2004..This gene encodes 3-beta-hydroxysterol Delta-24-reductase (DHCR24), which converts desmosterol into cholesterol...
- Specificity of the commonly used enzymatic assay for plasma cholesterol determinationM H Moghadasian
Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, Canada
J Clin Pathol 55:859-61. 2002..To assess the specificity and sensitivity of the commonly used enzymatic colorimetric test for plasma cholesterol determination...
- Changes in cholesterol biosynthetic and transport pathways after excitotoxicityJi Hyun Kim
Department of Anatomy, National University of Singapore, Singapore
J Neurochem 112:34-41. 2010..GC-MS analyses showed a significant increase in cholesterol biosynthetic precursors lanosterol, desmosterol and 7-dehydrocholesterol at 1 day after kainate injection presumably reflecting biosysnthesis in injured ..
- Lipid, FA, and sterol composition of New Zealand green lipped mussel (Perna canaliculus) and Tasmanian blue mussel (Mytilus edulis)Karen J Murphy
RMIT University, Melbourne, Victoria, Australia
Lipids 37:587-95. 2002..Other major sterols included brassicasterol, 22-methylcholesterol, trans-22-dehydrocholesterol, and desmosterol. There were significant differences (P < 0.05) between the NZGLM and TBM for 12 of the 20 sterols measured...
- Subcellular organelle lipidomics in TLR-4-activated macrophagesAlexander Y Andreyev
Department of Chemistry and Biochemistry and Department of Pharmacology, University of California, San Diego, CA 92093, USA
J Lipid Res 51:2785-97. 2010..g., increases in the levels of ceramides and the cholesterol precursors desmosterol and lanosterol). Other changes were manifest in specific organelles...
- Heritability of plasma noncholesterol sterols and relationship to DNA sequence polymorphism in ABCG5 and ABCG8Knut E Berge
The Department of Molecular Genetics, UT Southwestern Medical Center, Dallas, TX 75390 9052, USA
J Lipid Res 43:486-94. 2002..derivative of cholesterol (cholestanol), two precursors in the cholesterol biosynthesis pathway (desmosterol and lathosterol), and two phytosterols (campesterol and sitosterol)...
- Increased expression of aquaporin-3 in the epidermis of DHCR24 knockout miceR Mirza
Division of Stress Recognition and Response, Department of Endocrinology, Research Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Nagoya, Japan
Br J Dermatol 158:679-84. 2008The DHCR24 (3beta-hydroxysterol-Delta24 reductase) gene encodes an enzyme catalysing conversion of desmosterol to cholesterol...
- Oxysteroids: a new class of steroids with autocrine and paracrine functionsNorman B Javitt
Dept of Pediatrics and Medicine, NYU School of Medicine, New York, N Y 10016, USA
Trends Endocrinol Metab 15:393-7. 2004..An additional three oxysteroids, the 25,26-hydroxy derivatives of lanosterol, zymosterol, and desmosterol, respectively, were generated in vitro by CYP27A1-transfected bacteria...
- Adaptation of cholesterol synthesis to fasting and TNF-alpha: profiling cholesterol intermediates in the liver, brain, and testisKlementina Fon Tacer
Center for Functional Genomic and Biochips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Zaloska 4, SI 1000 Ljubljana, Slovenia
J Steroid Biochem Mol Biol 121:619-25. 2010..5% in the liver, 1% in brain and 5% in testis. In the liver only 7-dehydrocholesterol, lanosterol and desmosterol were detected...
- Secretion of sterols and the NPC2 protein from primary astrocytesAino Liisa Mutka
Institute of Biotechnology, University of Helsinki, Viikinkaari 9, 00014 University of Helsinki, Finland
J Biol Chem 279:48654-62. 2004..murine embryonic astrocytes secrete endogenously synthesized cholesterol but also the cholesterol precursors desmosterol and lathosterol. In astrocyte membranes, desmosterol and cholesterol were the predominant sterols...
- Polymorphisms in the ABCG5 and ABCG8 genes associate with cholesterol absorption and insulin sensitivityHelena Gylling
Department of Clinical Nutrition, University of Kuopio, Kuopio University Hospital, Kuopio, Finland
J Lipid Res 45:1660-5. 2004..index (BMI), plasma glucose, serum insulin and triglycerides, and cholesterol synthesis markers (cholestenol, desmosterol, lathosterol) and low HDL cholesterol and cholesterol absorption markers (campesterol, sitosterol) (P < 0...
- Loss of apolipoprotein E exacerbates the neonatal lethality of the Smith-Lemli-Opitz syndrome mouseCurzio Solcà
Division of Endocrinology, Metabolism and Clinical Nutrition, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Mol Genet Metab 91:7-14. 2007..mutations in the enzyme 3ss-hydroxysterol Delta7 reductase (Dhcr7) lead to a failure of cholesterol (and desmosterol) synthesis, with an accumulation of precursor sterols, such as 7-dehydrocholesterol...
- DHCR24-knockout embryonic fibroblasts are susceptible to serum withdrawal-induced apoptosis because of dysfunction of caveolae and insulin-Akt-Bad signalingXiuli Lu
Department of Endocrinology and Metabolism, Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464 8601, Japan
Endocrinology 147:3123-32. 2006The DHCR24 gene encodes an enzyme catalyzing the last step of cholesterol biosynthesis, the conversion of desmosterol to cholesterol...
- Synthesis and in vitro biological activity of 4alpha-(2-propenyl)-5alpha-cholest-24-en-3alpha-ol: a 24,25-dehydro analog of the hypocholesterolemic agent 4alpha-(2-propenyl)-5alpha-cholestan-3alpha-olH S Lin
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
Steroids 64:217-27. 1999..To investigate the effect of the 24,25-unsaturation in the D-ring side chain (desmosterol D-ring side chain) on antagonizing the repressing effect of 25-hydroxycholesterol, 4alpha-(2-propenyl)-5alpha-..
- Combined gas chromatographic/mass spectrometric analysis of cholesterol precursors and plant sterols in cultured cellsJure Acimovic
Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
J Chromatogr B Analyt Technol Biomed Life Sci 877:2081-6. 2009..allowing quantitative analysis of 11 structurally similar cholesterol precursors and plant sterols (squalene, desmosterol, 7-dehydrocholesterol, lathosterol, zymosterol, dihydro-lanosterol, lanosterol, FF-MAS, T-MAS, campesterol, ..
- Interannual variations in the lipids of the Antarctic pteropods Clione limacina and Clio pyramidataC F Phleger
Department of Biology, San Diego State University, San Diego, CA 92182, USA
Comp Biochem Physiol B Biochem Mol Biol 128:553-64. 2001..of both pteropods included trans-dehydrocholesterol, brassicasterol, 24-methylenecholesterol, cholesterol and desmosterol. Levels of 24-methylenecholesterol and desmosterol were lower in both pteropods in 1997 compared to 1998. C...
- Seladin-1 is a fundamental mediator of the neuroprotective effects of estrogen in human neuroblast long-term cell culturesPaola Luciani
Endocrine Unit, Department of Clinical Physiopathology, Center for Research, Transfer and High Education on Chronic Inflammatory, Degenerative and Neoplastic Disorders for the Development of Novel Therapies, University of Florence, Florence, Italy
Endocrinology 149:4256-66. 2008..seladin-1 gene encodes 3beta-hydroxysterol Delta24-reductase, which catalyzes the synthesis of cholesterol from desmosterol. We have demonstrated previously that in fetal neuroepithelial cells, 17beta-estradiol (17betaE2), raloxifene, ..
- Profile of cholesterol-related sterols in aged amyloid precursor protein transgenic mouse brainDieter Lutjohann
Department of Clinical Pharmacology, University of Bonn, Sigmund Freud Strasse 25, D 53105 Bonn, Germany
J Lipid Res 43:1078-85. 2002..the sterols measured, lanosterol is the first common intermediate of two different pathways, which use either desmosterol or lathosterol as the predominant precursors for de novo synthesis of brain cholesterol...
- Cholesterol absorption decreases after Roux-en-Y gastric bypass but not after gastric bandingJussi Pihlajamaki
Department of Medicine, University of Kuopio and Kuopio University Hospital, 70210 Kuopio, Finland
Metabolism 59:866-72. 2010..Serum markers of cholesterol synthesis (cholestenol, desmosterol, and lathosterol) and cholesterol absorption (campesterol, sitosterol, avenasterol, and cholestanol) were ..
- Recent insights into the Smith-Lemli-Opitz syndromeH Yu
Division of Endocrinology, Metabolism and Nutrition, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Clin Genet 68:383-91. 2005..DHCR7 deficiency impairs both cholesterol and desmosterol production, resulting in elevated 7DHC/8DHC levels, typically decreased cholesterol levels and, importantly, ..
- Insulin resistance is associated with increased cholesterol synthesis and decreased cholesterol absorption in normoglycemic menJussi Pihlajamaki
Department of Medicine, University of Kuopio, Kuopio, Finland
J Lipid Res 45:507-12. 2004..05). Fasting insulin correlated with desmosterol (r = 0.286, P = 0.015) and lathosterol (r = 0.248, P = 0...
- Decrease in order of human sperm lipids during capacitationNicholas L Cross
Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA
Biol Reprod 69:529-34. 2003..Loss of the sperm sterols, cholesterol and desmosterol, is an obligatory step in the capacitation of human sperm...
- Sperm membrane fatty acid composition in the Eastern grey kangaroo (Macropus giganteus), koala (Phascolarctos cinereus), and common wombat (Vombatus ursinus) and its relationship to cold shock injury and cryopreservation successR R Miller
Biology Department, Hillsdale College, Hillsdale, MI, USA
Cryobiology 49:137-48. 2004..While species-related differences in membrane sterol levels (cholesterol and desmosterol) were observed in cauda epididymidal spermatozoa, marsupial membrane sterol levels are very low...
- Reductions in cholesterol and synaptic markers in association cortex in mood disordersClare L Beasley
Center for Complex Disorders, Department of Psychiatry, University of British Columbia, Vancouver, Canada
Bipolar Disord 7:449-55. 2005..The aim of this study was to quantify sterol levels in the brains of patients with major psychiatric disorders and further to relate these levels to markers of myelin and synapses...
- SREBP-1c gene polymorphism is associated with increased inhibition of cholesterol-absorption in response to ezetimibe treatmentH K Berthold
Medical Faculty of the University of Bonn, Clinical Pharmacology, Bonn, Germany
Exp Clin Endocrinol Diabetes 116:262-7. 2008....
- Cholesterol absorption and synthesis in pediatric kidney, liver, and heart transplant recipientsArja Siirtola
Pediatric Research Center, University of Tampere, Tampere, Finland
Transplantation 81:327-34. 2006..To find an explanation to the observed difference, our kidney recipients' cholesterol synthesis and absorption efficiency was compared to those of liver and heart recipients...
- Regulation of cholesterol 25-hydroxylase expression by vitamin D3 metabolites in human prostate stromal cellsJing Huan Wang
Department of Anatomy, Medical School, 33014 University of Tampere, Tampere, Finland
Biochem Biophys Res Commun 345:720-5. 2006..25-Hydroxycholesterol significantly decreased the P29SN cell number. A CH25H enzyme inhibitor, desmosterol, increases basal cell number but has no significant effect on vitamin D3 treated cells...
- The cholesterol storage disorder of the mutant BALB/c mouse. A primary genetic lesion closely linked to defective esterification of exogenously derived cholesterol and its relationship to human type C Niemann-Pick diseaseP G Pentchev
J Biol Chem 261:2772-7. 1986..Homozygous-affected fibroblasts took up and converted [3H]desmosterol to [3H]cholesterol at a normal rate indicating that the murine mutation does not compromise the transport of ..
- [Seladin-1/DHCR24: a key protein of cell homeostasis and cholesterol biosynthesis]Joanna Drzewinska
Katedra Biofizyki Molekularnej, Uniwersytet Łódzki, 90 237 Łódź
Postepy Hig Med Dosw (Online) 63:318-30. 2009..of DHCR24 gene result in inhibition of the enzymatic activity of seladin-1, causing an accumulation of desmosterol and leading to a lethal disorder called desmosterolosis...
- Neuronal differentiation of human mesenchymal stem cells: changes in the expression of the Alzheimer's disease-related gene seladin-1Susanna Benvenuti
Endocrine Unit, Department of Clinical Physiopathology, University of Florence, 50139 Florence, Italy
Exp Cell Res 312:2592-604. 2006..In addition, seladin-1 catalyzes the conversion of desmosterol into cholesterol...
- Differential effects of cholesterol and its immediate biosynthetic precursors on membrane organizationSandeep Shrivastava
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India
Biochemistry 47:5668-77. 2008..7-Dehydrocholesterol (7-DHC) and desmosterol are the immediate biosynthetic precursors of cholesterol in the Kandutsch-Russell and Bloch pathway...
- Alterations in brain cholesterol metabolism in the APPSLxPS1mut mouse, a model for Alzheimer's diseaseTim Vanmierlo
Department of Neuroscience, Maastricht University, Maastricht, The Netherlands
J Alzheimers Dis 19:117-27. 2010..age of 9 months when AD-like pathology starts to develop, display increased levels of the cholesterol precursor desmosterol and of the cholesterol metabolite 27-hydroxy(OH)cholesterol in their cerebellum in comparison with wild-type ..
- Regulation of hippocampal cholesterol metabolism by apoE and environmental stimulationOfir Levi
Department of Neurobiochemistry, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
J Neurochem 95:987-97. 2005..In contrast, the hippocampal levels of cholesterol and its precursors lathosterol and desmosterol and metabolite 24S-hydroxycholesterol were lower in apoE-deficient mice that were maintained in a regular ..
- New insights on the neuroprotective role of sterols and sex steroids: the seladin-1/DHCR24 paradigmAlessandro Peri
Department of Clinical Physiopathology, Endocrine Unit, Center for Research, University of Florence, Florence, Italy
Front Neuroendocrinol 30:119-29. 2009..that encodes 3-beta-hydroxysterol delta-24-reductase (DHCR24), that catalyzes the synthesis of cholesterol from desmosterol. There is evidence that cholesterol plays a fundamental role in maintaining brain homeostasis...
- The reduced form of coenzyme Q10 mediates distinct effects on cholesterol metabolism at the transcriptional and metabolite level in SAMP1 miceConstance Schmelzer
Department of Molecular Prevention, Institute of Human Nutrition and Food Science, Molecular Prevention, Christian Albrechts University of Kiel, Kiel, Germany
IUBMB Life 62:812-8. 2010..We found a significant increase of desmosterol (2.0-fold, P < 0.001) in the liver of ubiquinol-supplemented SAMP1 mice when related to control animals...
- Studies on the transcriptional regulation of cholesterol 24-hydroxylase (CYP46A1): marked insensitivity toward different regulatory axesYoshihiko Ohyama
Divisions of Clinical Chemistry and Clinical Pharmacology, Department of Laboratory Medicine, Karolinska University Hospital, Huddinge, Stockholm, Sweden
J Biol Chem 281:3810-20. 2006..in vivo in a sterol-deficient mouse model (Dhcr24 null) in which almost all cholesterol had been replaced with desmosterol, which is not a substrate for CYP46A1...
- Apolipoprotein E phenotype regulates cholesterol absorption in healthy 13-month-old children--The STRIP StudyA Tammi
Research Centre of Applied and Preventive Cardiovascular Medicine, University of Turku, Kiinamyllynkatu 10, FIN 20520 Turku, Finland
Pediatr Res 50:688-91. 2001..early childhood, we measured serum plant sterol (campesterol and sitosterol) and cholesterol precursor sterol (desmosterol and lathosterol) concentrations in healthy 13-month old children using gas-liquid chromatography...
- Lipids and buoyancy in Southern ocean pteropodsC F Phleger
Department of Biology, San Diego State University, California 92182, USA
Lipids 32:1093-100. 1997..as are the sterols, which include 24-methylenecholesterol, transdehydrocholesterol, cholesterol, and desmosterol. This finding probably reflects the fact that Limacina is the major food source for Clione...
- Discovering oxysterols in plasma: a window on the metabolomeWilliam J Griffiths
Institute of Mass Spectrometry, School of Medicine, Grove Building, Swansea University, Singleton Park, Swansea, UK
J Proteome Res 7:3602-12. 2008..is also applicable for the analysis of other sterols in plasma, that is, cholesterol, 7-dehydrocholesterol, and desmosterol, as well as cholesterol 5,6- seco-sterols and steroid hormones...
- Neuroprotective effects of the Alzheimer's disease-related gene seladin-1Alessandro Peri
Endocrine Unit, Department of Clinical Physiopathology, University of Florence, 50139 Florence, Italy
J Mol Endocrinol 41:251-61. 2008..gene encodes 3-beta-hydroxysterol Delta-24-reductase, which catalyzes the synthesis of cholesterol from desmosterol. In recent years, it has been demonstrated that an appropriate amount of membrane cholesterol determines the ..
- Polymorphism of the hepatic influx transporter organic anion transporting polypeptide 1B1 is associated with increased cholesterol synthesis rateMarja K Pasanen
Department of Clinical Pharmacology, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland
Pharmacogenet Genomics 18:921-6. 2008..The mean fasting baseline plasma desmosterol to cholesterol ratio was 40% higher in participants with the SLCO1B1 c...
- Formal synthesis of squalamine from desmosterolKazuo Okumura
Niigata University of Pharmacy and Applied Life Sciences, Japan
Chem Pharm Bull (Tokyo) 51:1177-82. 2003..was synthesized from the 3-O-acetyl-24R,25-dihydroxy derivative of desmosterol via 10 steps in 16% overall yield and squalamine was also prepared via two further steps in 7...
- Enzyme inhibition during the conversion of squalene to cholesterolD Lewis
Department of Cardiovascular Biology, Rhone Poulenc Rorer Central Research, Collegeville, PA 19426 0107, USA
Steroids 60:475-83. 1995..second assay detects accumulation of any of five intermediates (squalene oxide, squalene dioxide, lanosterol, desmosterol, and 7-dehydrocholesterol) upon inhibition of enzymes catalyzing reactions in the conversion of squalene to ..
- Alteration of cholesterol biosynthetic pathways in the skin of mice administered polycyclic aromatic hydrocarbonsK Fukao
Department of Oral and Maxillofacial Surgery I, Hiroshima University, School of Dentistry, Japan
Cancer Res 48:2555-60. 1988..When diazacholesterol (a metabolic inhibitor) was administered to mice, both desmosterol and 5 alpha-cholesta-7,24-dien-3 beta-ol accumulated in the skin, whereas the level of lathosterol decreased...
- Serum plant and other noncholesterol sterols, cholesterol metabolism and 22-year mortality among middle-aged menTimo E Strandberg
Institute of Health Sciences Geriatrics, University of Oulu, and Oulu University Hospital, Unit of General Practice, PO Box 5000, FIN 90014, Oulu, Finland
Atherosclerosis 210:282-7. 2010..To evaluate long-term prognostic effect of serum noncholesterol sterols, including plant sterols, in middle-aged men with high cardiovascular disease (CVD) risk, without statins at baseline...
- Correlation between serum levels of some cholesterol precursors and activity of HMG-CoA reductase in human liverI Bjorkhem
Department of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden
J Lipid Res 28:1137-43. 1987..beta-ol), two lathosterols (5 alpha-cholest-7-en-3 beta-ol and 5 alpha-cholest-8-en-3 beta-ol) and desmosterol (cholest-5, 24-dien-3 beta-ol) were measured in untreated patients (n = 7) and patients treated with ..
- Stereochemistry of hydrogen addition to C-25 of desmosterol by sterol-delta 24-reductase of rat liver homogenateT Yagi
Kyoritsu College of Pharmacy, Tokyo, Japan
Chem Pharm Bull (Tokyo) 42:680-2. 1994Chemically synthesized (26)- and (27)-methyl-13C-labeled desmosterol (3, 5) were separately incubated with rat liver homogenate...
- Cerebral accumulation of dietary derivable plant sterols does not interfere with memory and anxiety related behavior in Abcg5-/- miceTim Vanmierlo
Department of Neuroscience, Maastricht University, Universiteitssingel 50, 6229, ER, Maastricht, The Netherlands
Plant Foods Hum Nutr 66:149-56. 2011..001). Plant sterol accumulation resulted in decreased levels of desmosterol (P<0.01) and 24(S)-hydroxycholesterol (P<0...
- Identification and potency of cyclodextrin-lipid inhibitors of Staphylococcus aureus α-toxinAnastasia C Weeks
Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA
Curr Eye Res 37:87-93. 2012..Staphylococcus aureus, a leading cause of bacterial keratitis, secretes α-toxin, a cytotoxin active on the corneal epithelium. This study describes the production and testing of chemical inhibitors of α-toxin action...
- Predicting the concentration range of unmonitored chemicals in wastewater-dominated streams and in run-off from biosolids-amended soilsBipin P Chari
Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, 1001 S McAllister Avenue, Tempe, AZ 85287 5701, USA
Sci Total Environ 440:314-20. 2012..and removal efficiencies of 10 sterols (campesterol, β-sitosterol, stigmasterol, β-stigmastanol, cholesterol, desmosterol, cholestanol, coprostanol, epicoprostanol, and ergosterol) as well as the putative toxicity posed by four ..
- Non-cholesterol sterol levels predict hyperglycemia and conversion to type 2 diabetes in Finnish menHenna Cederberg
Department of Medicine, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland
PLoS ONE 8:e67406. 2013..A cholesterol synthesis marker desmosterol significantly predicted an increase, and two absorption markers (campesterol and avenasterol) a decrease in the ..
- REGULATION OF SPERM EXOCYTOSISNicholas Cross; Fiscal Year: 2002..The cholesterol content of freshly ejaculated and oviductal bovine sperm will be compared to see if sperm lose cholesterol in the female tract. ..
- APOLIPOPROTEIN ISOFORMS AND RETINAL DEGENERATIONSTEVEN FLIESLER; Fiscal Year: 2007....
- Significance of CYP46A1 and other P450s in retinal functionIrina Pikuleva; Fiscal Year: 2007..Relevance (for lay audience). This research will be critical for understanding the development of certain eye diseases that reduce vision. ..
- Cholesterol-metabolizing P450s and Alzheimer's diseaseIrina Pikuleva; Fiscal Year: 2007..If funded, the release time and salary support will significantly enhance the P.I.'s career in basic science research and make it more medically oriented. ..
- Enzyme Targets-Sterol Synthesis-Opportunistic PathogensWILLIAM NES; Fiscal Year: 2004..The results from these studies will facilitate the design of therapeutic strategies aimed to provide species-specific discrimination of inhibition in the ergosterol biosynthetic pathway. ..