diglycerides

Summary

Top Publications

  1. ncbi Mechanism by which fatty acids inhibit insulin activation of insulin receptor substrate-1 (IRS-1)-associated phosphatidylinositol 3-kinase activity in muscle
    Chunli Yu
    Department of Internal Medicine, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 277:50230-6. 2002
  2. ncbi Diacylglycerol, when simplicity becomes complex
    Silvia Carrasco
    Department Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, Darwin 3, UAM Campus Cantoblanco, Madrid E 28049, Spain
    Trends Biochem Sci 32:27-36. 2007
  3. ncbi Shaping up the membrane: diacylglycerol coordinates spatial orientation of signaling
    María Almena
    Department of Immunology and Oncology, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, E 28049 Madrid, Spain
    Trends Biochem Sci 36:593-603. 2011
  4. pmc Role of diacylglycerol kinase alpha in the attenuation of receptor signaling
    M A Sanjuan
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    J Cell Biol 153:207-20. 2001
  5. ncbi Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs
    Jeong Seop Rhee
    Abteilung Membranbiophysik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Bundesrepublik Deutschland
    Cell 108:121-33. 2002
  6. ncbi Disruption of diacylglycerol metabolism impairs the induction of T cell anergy
    Benjamin A Olenchock
    Signal Transduction Program, Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nat Immunol 7:1174-81. 2006
  7. pmc Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism
    M K Topham
    The Huntsman Cancer Institute and Department of Internal Medicine, University of Utah, Salt Lake City, Utah 84112, USA
    J Cell Biol 152:1135-43. 2001
  8. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004
  9. ncbi Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals
    E Oancea
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 95:307-18. 1998
  10. pmc Increased diacylglycerols characterize hepatic lipid changes in progression of human nonalcoholic fatty liver disease; comparison to a murine model
    D Lee Gorden
    Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 6:e22775. 2011

Research Grants

Detail Information

Publications261 found, 100 shown here

  1. ncbi Mechanism by which fatty acids inhibit insulin activation of insulin receptor substrate-1 (IRS-1)-associated phosphatidylinositol 3-kinase activity in muscle
    Chunli Yu
    Department of Internal Medicine, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 277:50230-6. 2002
    ..This in turn leads to decreased IRS-1 tyrosine phosphorylation and decreased activation of IRS-1-associated PI3-kinase activity resulting in decreased insulin-stimulated glucose transport activity...
  2. ncbi Diacylglycerol, when simplicity becomes complex
    Silvia Carrasco
    Department Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, Darwin 3, UAM Campus Cantoblanco, Madrid E 28049, Spain
    Trends Biochem Sci 32:27-36. 2007
    ..Recent discoveries are also delineating the complex and strategic role of DAG in regulating biochemical networks...
  3. ncbi Shaping up the membrane: diacylglycerol coordinates spatial orientation of signaling
    María Almena
    Department of Immunology and Oncology, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, E 28049 Madrid, Spain
    Trends Biochem Sci 36:593-603. 2011
    ..This review brings together the most recent studies, emphasizing the contribution of compartmentalized DAG metabolism to orientated signaling events...
  4. pmc Role of diacylglycerol kinase alpha in the attenuation of receptor signaling
    M A Sanjuan
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    J Cell Biol 153:207-20. 2001
    ..This study is the first to describe DGKalpha as an integral component of the signaling cascades that link plasma membrane receptors to nuclear responses...
  5. ncbi Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs
    Jeong Seop Rhee
    Abteilung Membranbiophysik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Bundesrepublik Deutschland
    Cell 108:121-33. 2002
    ..Modulation of Munc13-1 activity by second messengers via the DAG/beta phorbol ester binding C(1) domain is essential for use-dependent alterations of synaptic efficacy and survival...
  6. ncbi Disruption of diacylglycerol metabolism impairs the induction of T cell anergy
    Benjamin A Olenchock
    Signal Transduction Program, Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nat Immunol 7:1174-81. 2006
    ..Our findings suggest that regulation of diacylglycerol metabolism is critical in determining whether activation or anergy ensues after T cell receptor stimulation...
  7. pmc Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism
    M K Topham
    The Huntsman Cancer Institute and Department of Internal Medicine, University of Utah, Salt Lake City, Utah 84112, USA
    J Cell Biol 152:1135-43. 2001
    ..Thus, we have identified a novel way to regulate Ras activation: through DGK zeta, which controls local accumulation of DAG that would otherwise activate RasGRP...
  8. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004
    ....
  9. ncbi Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals
    E Oancea
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 95:307-18. 1998
    ..The properties of this molecular decoding machine make PKCgamma responsive to persistent diacylglycerol increases combined with high- but not low-frequency calcium spikes...
  10. pmc Increased diacylglycerols characterize hepatic lipid changes in progression of human nonalcoholic fatty liver disease; comparison to a murine model
    D Lee Gorden
    Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 6:e22775. 2011
    ..The goal of this study was to characterize changes in lipid species through progression of human NAFLD using advanced lipidomic technology and compare this with a murine model of early and advanced NAFLD...
  11. ncbi In situ lipidomic analysis of nonalcoholic fatty liver by cluster TOF-SIMS imaging
    Delphine Debois
    Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Anal Chem 81:2823-31. 2009
    ....
  12. ncbi Activation of ADP-ribosylation factor 1 GTPase-activating protein by phosphatidylcholine-derived diacylglycerols
    B Antonny
    CNRS, Institut de Pharmacologie Moleculaire et Cellulaire, 660 route des Lucioles, 06560 Valbonne, France
    J Biol Chem 272:30848-51. 1997
    ..Thus, the phospholipase D pathway may provide a feedback mechanism that promotes GTP hydrolysis on ARF1 and the consequent uncoating of vesicles...
  13. pmc Insulin resistance is associated with higher intramyocellular triglycerides in type I but not type II myocytes concomitant with higher ceramide content
    Paul M Coen
    Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 59:80-8. 2010
    ....
  14. pmc Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver
    Anna Kotronen
    Department of Medicine, Division of Diabetes, Helsinki, Finland
    Diabetes 58:203-8. 2009
    ..To determine whether 1) hepatic ceramide and diacylglycerol concentrations, 2) SCD1 activity, and 3) hepatic lipogenic index are increased in the human nonalcoholic fatty liver...
  15. ncbi The diacylgylcerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance
    Alexander Dietrich
    Institut fur Pharmakologie und Toxikologie, PhilippsUniversität Marburg, Karl von Frisch Str 1, 35043 Marburg, Germany
    Pflugers Arch 451:72-80. 2005
    ....
  16. pmc A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase
    Eleftherios Karanasios
    Cambridge Institute for Medical Research, University of Cambridge Wellcome Trust Medical Research Council Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 107:17539-44. 2010
    ..Our data suggest that dephosphorylation of Pah1p by the Nem1p-Spo7p complex enables the amphipathic helix to anchor Pah1p onto the nuclear/ER membrane allowing the production of DAG for lipid biosynthesis...
  17. ncbi Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol
    T Hofmann
    Institut fur Pharmakologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Germany
    Nature 397:259-63. 1999
    ..Thus, hTRPC3 and hTRPC6 represent the first members of a new functional family of second-messenger-operated cation channels, which are activated by diacylglycerol...
  18. ncbi Diacylglycerols, multivalent membrane modulators
    Juan C Gomez-Fernandez
    Departamento de Bioquimica y Biologia Molecular A, Facultad de Veterinaria, Universidad de Murcia, Apartado de Correos 4021, Murcia, Spain
    Chem Phys Lipids 148:1-25. 2007
    ....
  19. ncbi DAG accumulation from saturated fatty acids desensitizes insulin stimulation of glucose uptake in muscle cells
    E Montell
    , Universitat de Barcelona, , 08028 Barcelona, Spain
    Am J Physiol Endocrinol Metab 280:E229-37. 2001
    ..Therefore, our results suggest that accumulation of DAG may be a molecular link between an increased availability of saturated FA and the induction of insulin resistance...
  20. pmc Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis
    R J Botelho
    Cell Biology Program, Hospital for Sick Children, and Department of Biochemistry, University of Toronto, Ontario M5G 1X8, Canada
    J Cell Biol 151:1353-68. 2000
    ....
  21. ncbi Diacylglycerol--when is it an intracellular messenger?
    M J Wakelam
    Institute for Cancer Studies, Birmingham University, Queen Elizabeth Hospital, Clinical Research Block, Birmingham B15 2TA, UK
    Biochim Biophys Acta 1436:117-26. 1998
    ..The evidence that it is the polyunsaturated forms of sn-1,2-diacylglycerol, but the more saturated forms of phosphatidate which function as intracellular signals is considered...
  22. ncbi Phospholipase Cbeta serves as a coincidence detector through its Ca2+ dependency for triggering retrograde endocannabinoid signal
    Yuki Hashimotodani
    Department of Cellular Neurophysiology, Graduate School of Medical Science, Kanazawa University, Kanazawa 920 8640, Japan
    Neuron 45:257-68. 2005
    ..We therefore conclude that PLCbeta1 serves as a coincidence detector through its Ca(2+) dependency for endocannabinoid release in hippocampal neurons...
  23. ncbi The cellular functions of the yeast lipin homolog PAH1p are dependent on its phosphatidate phosphatase activity
    Gil Soo Han
    Department of Food Science and the Rutgers Center for Lipid Research, Rutgers University, New Brunswick, New Jersey 08901, USA
    J Biol Chem 282:37026-35. 2007
    ..These results demonstrated that the PAP1 activity of the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology...
  24. pmc Contrasting effects of fish oil and safflower oil on hepatic peroxisomal and tissue lipid content
    Susanne Neschen
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520 8020, USA
    Am J Physiol Endocrinol Metab 282:E395-401. 2002
    ..In turn, an increased hepatic beta-oxidative capacity results in lower hepatic triglyceride/diacylglycerol and intramyocellular triglyceride/fatty acyl-CoA content...
  25. ncbi Regulation of DGK-theta
    Becky Tu-Sekine
    Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Cell Physiol 220:548-52. 2009
    ..This review presents the progress on the characterization of a primarily neuronal isoform DGK-theta, and examines current data on the primary structure, regulation and potential cellular functions of this enzyme...
  26. ncbi Diacylglycerol kinase-theta is localized in the speckle domains of the nucleus
    Giovanna Tabellini
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Sezione di Anatomia Umana, Cell Signalling Laboratory, Universita di Bologna, 40126, Bologna, Italy
    Exp Cell Res 287:143-54. 2003
    ..We may speculate that nuclear speckle-located DGK-theta, on cell stimulation with an agonist, converts to PA the DAG derived from PLCbeta1-dependent PIP(2) hydrolysis...
  27. pmc A novel cross-talk in diacylglycerol signaling: the Rac-GAP beta2-chimaerin is negatively regulated by protein kinase Cdelta-mediated phosphorylation
    Erin M Griner
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    J Biol Chem 285:16931-41. 2010
    ....
  28. ncbi Beta2-chimaerin provides a diacylglycerol-dependent mechanism for regulation of adhesion and chemotaxis of T cells
    María Siliceo
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, Cantoblanco, E 28049 Madrid, Spain
    J Cell Sci 119:141-52. 2006
    ..These studies demonstrate that beta2-chimaerin provides a novel, diacylglycerol-dependent mechanism for Rac regulation in T cells and suggest a functional role for this protein in Rac-mediated cytoskeletal remodeling...
  29. ncbi Ca2+ selectivity and fatty acid specificity of the noncapacitative, arachidonate-regulated Ca2+ (ARC) channels
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, New York 14642, USA
    J Biol Chem 278:10174-81. 2003
    ..Whether this involves a direct action of arachidonic acid on the channel protein itself or an action on some intermediary molecule is, at present, unclear...
  30. ncbi Receptor-operated Ca2+ entry mediated by TRPC3/TRPC6 proteins in rat prostate smooth muscle (PS1) cell line
    S Thebault
    Laboratoire de Physiologie Cellulaire, INSERM EMI 0228, France
    J Cell Physiol 204:320-8. 2005
    ..It may be thus concluded that PS1 cells express TRPC3 and TRPC6 proteins which function as receptor- and store-operated Ca(2+) entry pathways...
  31. pmc Tight regulation of diacylglycerol-mediated signaling is critical for proper invariant NKT cell development
    Shudan Shen
    Division of Allergy and Immunology, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 187:2122-9. 2011
    ..Moreover, constitutive IKKβ and Ras activities also result in iNKT developmental defects. Thus, DAG-mediated signaling is not only essential but also needs to be tightly regulated for proper iNKT cell development...
  32. ncbi Interleukin-1-induced ether-linked diglycerides inhibit calcium-insensitive protein kinase C isotypes. Implications for growth senescence
    A Mandal
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106-4949, USA
    J Biol Chem 272:20306-11. 1997
    It is hypothesized that inflammatory cytokines and vasoactive peptides stimulate distinct species of diglycerides that differentially regulate protein kinase C isotypes...
  33. ncbi Structural mechanism for lipid activation of the Rac-specific GAP, beta2-chimaerin
    Bertram Canagarajah
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892, USA
    Cell 119:407-18. 2004
    ..Phospholipid binding to the C1 domain triggers the cooperative dissociation of these interactions, allowing the N terminus to move out of the active site and thereby activating the enzyme...
  34. pmc Phospholipase Cgamma/diacylglycerol-dependent activation of beta2-chimaerin restricts EGF-induced Rac signaling
    HongBin Wang
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    EMBO J 25:2062-74. 2006
    ..Our results represent the first direct evidence of divergence in DAG signaling downstream of a tyrosine-kinase receptor via a PKC-independent mechanism...
  35. ncbi Signalling mechanisms for TRPC3 channels
    James W Putney
    Calcium Regulation Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    Novartis Found Symp 258:123-33; discussion 133-9, 155-9, 263-6. 2004
    ..However, in an avian pre-B cell line, TRPC3 can function either as a diacylglycerol-activated channel, or as a SOC. The mechanism of regulation of TRPC3 in this cell line appears to be related to the level of expression of the protein...
  36. pmc beta2-chimaerin is a novel target for diacylglycerol: binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain
    M J Caloca
    Center for Experimental Therapeutics, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Proc Natl Acad Sci U S A 96:11854-9. 1999
    ..Our results demonstrate that beta2-chimaerin is a high affinity receptor for DAG through binding to its C1 domain and supports the emerging concept that multiple pathways transduce signaling through DAG and the phorbol esters...
  37. ncbi The Na+/Ca2+ exchange inhibitor KB-R7943 potently blocks TRPC channels
    Robert Kraft
    Carl Ludwig Institute for Physiology, University Leipzig, Liebigstr 27, 04103 Leipzig, Germany
    Biochem Biophys Res Commun 361:230-6. 2007
    ..These data further suggest that the protective effects of KB-R7943 in ischemic tissue may, at least partly, be due to inhibition of TRPC channels...
  38. ncbi A diacylglycerol-gated cation channel in vomeronasal neuron dendrites is impaired in TRPC2 mutant mice: mechanism of pheromone transduction
    Philippe Lucas
    Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Neuron 40:551-61. 2003
    ..Thus, a previously unanticipated mechanism involving direct channel opening by DAG underlies the transduction of sensory cues in the accessory olfactory system...
  39. pmc Spatial regulation of membrane fusion controlled by modification of phosphoinositides
    Fabrice Dumas
    Cell Biophysics Laboratory, Lincoln s Inn Fields Laboratories, Cancer Research UK, London, United Kingdom
    PLoS ONE 5:e12208. 2010
    ..We discuss the general implications of membrane fusion regulation and spatial control utilising such a mechanism...
  40. ncbi Porcine diacylglycerol kinase sequence has zinc finger and E-F hand motifs
    F Sakane
    Department of Biochemistry, Sapporo Medical College, Japan
    Nature 344:345-8. 1990
    ..These properties of 80K DGK indicate that its action is probably linked with both of the second messengers diacylglycerol and inositol 1,4,5-trisphosphate...
  41. ncbi Protein kinase C activation: isozyme-specific effects on metabolism and cardiovascular complications in diabetes
    I Idris
    School of Medical and Surgical Sciences, University of Nottingham, Royal Infirmary, Derby, UK
    Diabetologia 44:659-73. 2001
    ..Thus, a greater understanding of the functional diversity and pathophysiological regulation of PKC isozymes is likely to have important clinical and therapeutic benefits...
  42. ncbi Mechanism of diacylglycerol-induced membrane targeting and activation of protein kinase Cdelta
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 279:29501-12. 2004
    ..Collectively, these results provide new insight into the differential roles of C1 domains in the DAG-induced membrane activation of PKCdelta and the origin of its specific subcellular localization in response to DAG...
  43. ncbi Calcium signaling in non-excitable cells: Ca2+ release and influx are independent events linked to two plasma membrane Ca2+ entry channels
    Ranjana Chakrabarti
    Department of Laboratory Medicine, St Michael s Hospital, 30 Bond Street, Toronto, Ontario, Canada M6N 4C5
    J Cell Biochem 99:1503-16. 2006
    ..The plasma membrane IP(3) receptor (IP(3)R) plays an essential role in Ca2+ influx, by interacting with the DAG-activated TRPC, without the requirement of binding to IP(3)...
  44. ncbi Sphingomyelin synthase as a potential target for D609-induced apoptosis in U937 human monocytic leukemia cells
    Aimin Meng
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, USA
    Exp Cell Res 292:385-92. 2004
    ..These results suggest that SMS is a potential target of D609 and inhibition of SMS may contribute to D609-induced tumor cell death via modulation of the cellular levels of ceramide and DAG...
  45. ncbi Dietary diacylglycerol suppresses high fat and high sucrose diet-induced body fat accumulation in C57BL/6J mice
    T Murase
    Biological Science Laboratories, Kao Corporation, 2606, Akabane, Ichikaimachi, Hagagun, Tochigi 321-3497, Japan
    J Lipid Res 42:372-8. 2001
    ..T. Mizuno, T. Omachi, K. Onizawa, Y. Komine, H. Kondo, T. Hase, and I. Tokimitsu. Dietary diacylglycerol suppresses high fat and high sucrose diet-induced body fat accumulation in C57BL/6J mice. J. Lipid Res. 2001. 42: 372--378...
  46. pmc The biology and biochemistry of diacylglycerol signalling. Meeting on molecular advances in diacylglycerol signalling
    Alex Toker
    Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, Massachusetts 02215, USA
    EMBO Rep 6:310-4. 2005
  47. ncbi Lipid-induced insulin resistance in human muscle is associated with changes in diacylglycerol, protein kinase C, and IkappaB-alpha
    Samar I Itani
    Diabetes Unit, Section of Endocrinology, Department of Medicine, Boston University Medical Center, Boston, MA, USA
    Diabetes 51:2005-11. 2002
    ..Whether acute FFA-induced insulin resistance in human skeletal muscle is caused by the activation of these specific PKC isoforms and the IKK-beta/IkappaB/NFkappaB pathway remains to be established...
  48. ncbi The PtdIns(4,5)P2 ligand itself influences the localization of PKCalpha in the plasma membrane of intact living cells
    Consuelo Marín-Vicente
    Dpto de Bioquímica y Biología Molecular A, Facultad de Veterinaria, Universidad de Murcia, Apdo 4021, E 30100 Murcia, Spain
    J Mol Biol 377:1038-52. 2008
    ....
  49. pmc Diacylglycerol enrichment of endoplasmic reticulum or lipid droplets recruits perilipin 3/TIP47 during lipid storage and mobilization
    James R Skinner
    Center for Human Nutrition, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:30941-8. 2009
    ..Membrane-permeable DG also drove perilipin 4 and 5 onto the ER. Together the data suggest that these lipid droplet proteins are recruited to DG-enriched membranes thereby linking lipid coat proteins to the metabolic state of the cell...
  50. ncbi Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands
    Hans Heinrich Oberg
    Institute of Immunology, University Hospital Schleswig Holstein, Campus Kiel, Kiel, Germany
    J Immunol 184:4733-40. 2010
    ..Moreover, our data indicate that a mixture of TLR2 ligands can be used to modulate human Treg activity...
  51. pmc DGK1-encoded diacylglycerol kinase activity is required for phospholipid synthesis during growth resumption from stationary phase in Saccharomyces cerevisiae
    Stylianos Fakas
    Department of Food Science and Rutgers Center for Lipid Research, Rutgers University, New Brunswick, New Jersey 08901, USA
    J Biol Chem 286:1464-74. 2011
    ..These observations, coupled to an inhibitory effect of dioctanoyl-diacylglycerol on the growth of wild type cells, indicated that diacylglycerol kinase also functions to alleviate diacylglycerol toxicity...
  52. pmc Effects of weight loss and exercise on insulin resistance, and intramyocellular triacylglycerol, diacylglycerol and ceramide
    J J Dubé
    Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh School of Medicine, University of Pittsburgh, 3459 Fifth Avenue, MUH N807, Pittsburgh, PA 15213, USA
    Diabetologia 54:1147-56. 2011
    ..This randomised repeated-measures study examined the effects of diet-induced weight loss (DIWL) and aerobic exercise (EX) on insulin sensitivity and intramyocellular triacylglycerol (IMTG), DAG and ceramide...
  53. ncbi Downregulation of diacylglycerol kinase delta contributes to hyperglycemia-induced insulin resistance
    Alexander V Chibalin
    Department of Molecular Medicine and Surgery, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Cell 132:375-86. 2008
    ..DGKdelta deficiency causes peripheral insulin resistance and metabolic inflexibility. These defects in glucose and energy homeostasis contribute to mild obesity later in life...
  54. ncbi Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling
    Lisa L Gallegos
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 281:30947-56. 2006
    ..Our data reveal that the magnitude and duration of PKC signaling is location-specific and controlled by the level of phosphatase activity and persistence of DAG at each location...
  55. ncbi Muscular diacylglycerol metabolism and insulin resistance
    Silvie Timmers
    Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht NUTRIM, Maastricht University, The Netherlands
    Physiol Behav 94:242-51. 2008
    ..Furthermore, we will discuss if and how modulation of skeletal muscle DAG levels could function as a possible therapeutic target for the treatment of type 2 diabetes mellitus...
  56. ncbi New insights into the regulation of protein kinase C and novel phorbol ester receptors
    D Ron
    Department of Neurology, Ernest Gallo Clinic and Research Center, University of California San Francisco, San Francisco, California 94110 3518, USA
    FASEB J 13:1658-76. 1999
    ....
  57. ncbi Diacylglycerol induces fusion of nuclear envelope membrane precursor vesicles
    Teresa Barona
    Biology Department, Amherst College, Amherst, Massachusetts 01002, USA
    J Biol Chem 280:41171-7. 2005
    ..This PI-PLC hydrolyzes a subset of sea urchin membrane vesicle PtdIns into diglycerides enriched in long chain, polyunsaturated species as revealed by a novel liquid chromatography-mass spectrometry ..
  58. ncbi Key role for ceramides in mediating insulin resistance in human muscle cells
    Laura Pickersgill
    Institute of Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 282:12583-9. 2007
    ..Furthermore, the triacylglycerol derived from oleate was able to exert a protective role in sequestering palmitate, thus preventing its conversion to ceramide...
  59. ncbi Rac-GAP-dependent inhibition of breast cancer cell proliferation by {beta}2-chimerin
    Chengfeng Yang
    Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
    J Biol Chem 280:24363-70. 2005
    ....
  60. ncbi Regulation of canonical transient receptor potential (TRPC) channel function by diacylglycerol and protein kinase C
    Kartik Venkatachalam
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 278:29031-40. 2003
    ..The profound yet distinct control by PKC and DAG of the activation of TRPC channel subtypes is likely the basis of a spectrum of regulatory phenotypes of expressed TRPC channels...
  61. pmc Protein kinase C function in muscle, liver, and beta-cells and its therapeutic implications for type 2 diabetes
    Carsten Schmitz-Peiffer
    Garvan Institute of Medical Research, Darlinghurst, Australia
    Diabetes 57:1774-83. 2008
  62. ncbi Imaging diacylglycerol dynamics at organelle membranes
    Moritoshi Sato
    Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Methods 3:797-9. 2006
    ..We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes...
  63. pmc The major sites of cellular phospholipid synthesis and molecular determinants of Fatty Acid and lipid head group specificity
    Annette L Henneberry
    The Atlantic Research Centre, Department of Pediatrics, IWK Health Centre, Dalhousie University, Halifax, Nova Scotia, B3H 4H7 Canada
    Mol Biol Cell 13:3148-61. 2002
    ....
  64. pmc Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels
    Byung Chang Suh
    Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 314:1454-7. 2006
    ..Hence, the depletion of PI(4,5)P2 suffices to suppress current fully, and other second messengers are not needed. Our approach is ideally suited to study biological signaling networks involving membrane phosphoinositides...
  65. ncbi Accumulation of diacylglycerol in the Chlamydia inclusion vacuole: possible role in the inhibition of host cell apoptosis
    Shirley M L Tse
    Department of Pediatrics, Division of Rheumatology, The Hospital for Sick Children, University of Toronto, Toronto M5G 1X8, Canada
    J Biol Chem 280:25210-5. 2005
    ..The scavenging of pro-apoptotic factors may provide a novel mechanism whereby pathogens promote their own survival by extending the life of the host cells they infect...
  66. ncbi Diacylglycerol-induced membrane targeting and activation of protein kinase Cepsilon: mechanistic differences between protein kinases Cdelta and Cepsilon
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, 60607, USA
    J Biol Chem 280:19784-93. 2005
    ..Collectively, these results provide new insight into the differential activation mechanisms of PKCdelta and PKCepsilon based on different structural and functional properties of their C1 domains...
  67. pmc A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells
    Emily J Quann
    Immunology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Immunol 12:647-54. 2011
    ..Our results establish a previously uncharacterized role for PKC proteins in T cell polarity...
  68. ncbi Prevention of hepatic steatosis and hepatic insulin resistance in mitochondrial acyl-CoA:glycerol-sn-3-phosphate acyltransferase 1 knockout mice
    Susanne Neschen
    Howard Hughes Medical Institute, Department of Internal Medicine, Yale University School of Medicine, New Haven, CN 06520, USA
    Cell Metab 2:55-65. 2005
    ..These data suggest that mtGPAT1 might serve as a novel target for treatment of hepatic steatosis and hepatic insulin resistance and that long chain acyl-CoA's do not mediate fat-induced hepatic insulin resistance in this model...
  69. ncbi Protein kinase C and other diacylglycerol effectors in cancer
    Erin M Griner
    Department of Pharmacology and Institute for Translational Medicine and Therapeutics ITMAT, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Nat Rev Cancer 7:281-94. 2007
    ....
  70. ncbi Diacylglycerol regulates the plasma membrane calcium pump from human erythrocytes by direct interaction
    Maria C Pérez-Gordones
    Instituto de Biología Experimental IBE, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
    Arch Biochem Biophys 489:55-61. 2009
    ..Additionally, by different fluorescent approaches, we demonstrated a direct binding between PMCA and DAG. The results obtained in this work strongly suggest that DAG is a novel effector of the PMCA, acting by a direct interaction...
  71. ncbi Characterizing the effects of saturated fatty acids on insulin signaling and ceramide and diacylglycerol accumulation in 3T3-L1 adipocytes and C2C12 myotubes
    Jose Antonio Chavez
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523 1870, USA
    Arch Biochem Biophys 419:101-9. 2003
    ..Collectively these findings implicate excess delivery of long-chain fatty acids in the development of insulin resistance resulting from lipid oversupply to skeletal muscle...
  72. ncbi Amplification of Ca2+ signaling by diacylglycerol-mediated inositol 1,4,5-trisphosphate production
    Chihiro Hisatsune
    Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute BSI 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    J Biol Chem 280:11723-30. 2005
    ..These results suggest a novel physiological function of DAG, i.e. amplification of Ca2+ signaling by enhancing IP3 production via its positive feedback effect on PLC activity...
  73. ncbi Protein kinase C-theta (PKCtheta): it's all about location, location, location
    Amnon Altman
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121, USA
    Immunol Rev 192:53-63. 2003
    ..Additional work is required to identify the immediate targets of PKCtheta and its immune functions in vivo. This work is likely to validate PKCtheta as an attractive drug target...
  74. pmc Relationship between structures and biological activities of mycoplasmal diacylated lipopeptides and their recognition by toll-like receptors 2 and 6
    Tsugumi Okusawa
    Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Nishi 7, Kita 13, Kita kuSapporo 060 8586, Japan
    Infect Immun 72:1657-65. 2004
    ....
  75. ncbi Synthesis and anti-herpes simplex viral activity of monoglycosyl diglycerides
    Wicharn Janwitayanuchit
    Faculty of Pharmaceutical Sciences, Chulalongkorn University Bangkok, 10330, Thailand
    Phytochemistry 64:1253-64. 2003
    Based on the discovery of antiviral beta-galactosyl diglycerides from Clinacanthus nutans leaves, 19 monoglycosyl diglycerides were synthesized and examined for inhibitory effect on herpes simplex virus types 1 and 2 (HSV-1, HSV-2)...
  76. pmc Phosphatidylcholine synthesis influences the diacylglycerol homeostasis required for SEC14p-dependent Golgi function and cell growth
    A L Henneberry
    The Atlantic Research Centre, Departments of Pediatrics and Biochemistry and Molecular Biology, IWK Grace Health Centre, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    Mol Biol Cell 12:511-20. 2001
    ..Di8:0 diacylglcyerol did not alter endogenous phospholipid metabolic pathways, nor was it converted to di8:0 phosphatidic acid...
  77. pmc Protein kinase C {delta}-specific activity reporter reveals agonist-evoked nuclear activity controlled by Src family of kinases
    Taketoshi Kajimoto
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:41896-910. 2010
    ....
  78. ncbi Role of diacylglycerol in PKD recruitment to the TGN and protein transport to the plasma membrane
    Carole L Baron
    Section of Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Science 295:325-8. 2002
    ..Thus, a DAG-dependent, PKD-mediated signaling regulates the formation of transport carriers from the TGN in mammalian cells...
  79. ncbi Generation of diacylglycerol molecular species through the cell cycle: a role for 1-stearoyl, 2-arachidonyl glycerol in the activation of nuclear protein kinase C-betaII at G2/M
    Elizabeth M Deacon
    MRC Centre for Immune Regulation, Birmingham University, Birmingham B15 2TT, UK
    J Cell Sci 115:983-9. 2002
    ..We propose that PKC-betaII nuclear translocation during G(2)/M phase transition is mediated in part by generation of SAG at the nucleus...
  80. ncbi Chemoenzymatic synthesis and antimicrobial activity evaluation of monogalactosyl diglycerides
    Francesca Cateni
    Department of Pharmaceutical Sciences, University of Trieste, P zle Europa, 1, 34127 Trieste, TS, Italy
    Eur J Med Chem 43:210-21. 2008
    Monogalactosyl diglycerides with medium to long fatty acid acyl chains, were prepared and examined for antimicrobial activity against Gram positive, Gram negative bacteria and fungi...
  81. ncbi Diacylglycerol kinases in the regulation of dendritic spines
    Karam Kim
    Department of Biological Sciences and National Creative Research Initiative Center for Synaptogenesis, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Korea
    J Neurochem 112:577-87. 2010
    ..In addition, DGKzeta appears to form a multi-protein complex with functionally related proteins to organize efficient DAG and PA signaling pathways at excitatory synapses...
  82. pmc A self-limiting regulation of vasoconstrictor-activated TRPC3/C6/C7 channels coupled to PI(4,5)P₂-diacylglycerol signalling
    Yuko Imai
    Department of Physiology, School of Medicine, Fukuoka University, Fukuoka 814 0180, Japan
    J Physiol 590:1101-19. 2012
    ..These results demonstrate that TRPC3/C6/C7 channels are differentially regulated by depletion of PI(4,5)P₂, and that the bimodal signal produced by PLC activation controls these channels in a self-limiting manner...
  83. ncbi Phosphatidylcholine-specific phospholipase C regulates activation of RAW264.7 macrophage-like cells by lipopeptide JBT3002
    F Zhang
    Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA
    J Leukoc Biol 69:1060-6. 2001
    ..RAW264.7 cells treated with DAG analogue 1-oleoyl-2-acetyl-sn-glycerol, in the presence of interferon-gamma, produced TNF-alpha. These results suggested that activation of RAW264.7 cells by JBT3002 requires PC-PLC activity...
  84. ncbi A characterisation study on the application of inverted lyotropic phases for subcutaneous drug release
    Erik Rosenbaum
    Department of Chemistry, Umea University, Linneus v 10, S 901 87 Umea, Sweden
    Int J Pharm 388:52-7. 2010
    ..However, the lipid phases showed a prolonged release, spanning weeks, of the model drugs, which exhibit lipophilicity values ranging by four orders of magnitude...
  85. pmc Distinct effects of fatty acids on translocation of gamma- and epsilon-subspecies of protein kinase C
    Y Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Cell Biol 143:511-21. 1998
    ....
  86. ncbi Role of lipid transfer particle in delivery of diacylglycerol from midgut to lipophorin in larval Manduca sexta
    L E Canavoso
    Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, The University of Arizona, Tucson, AZ 85721-0088, USA
    Insect Biochem Mol Biol 31:783-90. 2001
    ..Under these conditions the amount of diacylglycerol transferred was a function of the LTP concentration. These are the first results showing that LTP is required to export diacylglycerol from the midgut to lipophorin...
  87. pmc Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes
    Silvia Carrasco
    Department of Immunology and Oncology, National Center for Biotechnology, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, E 28049 Madrid, Spain
    Mol Biol Cell 15:2932-42. 2004
    ..This is shown by analyzing the contribution of these two lipid pools to PLC-dependent Ras activation in response to TCR triggering...
  88. ncbi Possible enhancing activity of diacylglycerol on 4-nitroquinoline 1-oxide induced carcinogenesis of the tongue in human c-Ha-ras proto-oncogene transgenic rats
    Hiroyuki Tsuda
    Department of Molecular Toxicology, Nagoya City University Graduate School of Medical Sciences, Mizuho cho, Mizuho ku, Nagoya 467 8601, Japan
    Food Chem Toxicol 45:1013-9. 2007
    ..Tongue SCC induction of wild males and all females was not significant. The present results suggest that DAG oil may have enhancing and/or promotion potential for tongue carcinogenesis in male Tg featuring elevated ras expression...
  89. pmc Increased membrane affinity of the C1 domain of protein kinase Cdelta compensates for the lack of involvement of its C2 domain in membrane recruitment
    Jennifer R Giorgione
    Department of Pharmacology, University of California at San Diego, La Jolla, California 92093 0721, USA
    J Biol Chem 281:1660-9. 2006
    ....
  90. ncbi Dietary 1,3-diacylglycerol protects against diet-induced obesity and insulin resistance
    Shinichiro Saito
    The Division of Preventive Medicine and Nutrition, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Metabolism 56:1566-75. 2007
    ..These findings suggest that diets enriched in 1,3-DAG oil may reduce WTD-induced IR and body fat accumulation by suppressing gluconeogenesis in liver and stimulating fat oxidation in skeletal muscle...
  91. pmc A diacylglycerol-protein kinase C-RasGRP1 pathway directs Ras activation upon antigen receptor stimulation of T cells
    Jeroen P Roose
    Department of Medicine, UCSF, 533 Parnassus Avenue, Room U 330, San Francisco, CA 94143, USA
    Mol Cell Biol 25:4426-41. 2005
    ..Conversely, active PKCtheta depends on RasGRP1 sufficiency to effectively trigger downstream events. Last, DAG-PKC-RasGRP1-driven Ras-Erk activation in T cells is a unique signaling event, not simply compensated for by SOS activity...
  92. ncbi Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta
    Lilian E Canavoso
    Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, University of Arizona, Tucson, AZ 85721 0088, USA
    J Lipid Res 45:456-65. 2004
    ..Nevertheless, these results indicate that the main pathway for DAG transfer from lipophorin to fat body is via LTP and receptor-mediated processes...
  93. ncbi Effect of cholecystokinin octapeptide on diacylglycerol-PKC signaling pathway in rat pulmonary interstitial macrophages stimulated by lipopolysaccharide
    Shun jiang Xu
    Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China
    Acta Pharmacol Sin 26:1497-504. 2005
    ..To investigate the effect of cholecystokinin octapeptide (CCK-8) on the diacylglycerol-protein kinase C (DAG-PKC) signaling pathway in rat pulmonary interstitial macrophages (PIM) stimulated by lipopolysaccaride (LPS)...
  94. ncbi Interleukin-2 causes an increase in saturated/monounsaturated phosphatidic acid derived from 1,2-diacylglycerol and 1-O-alkyl-2-acylglycerol
    D R Jones
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Cantoblanco, 28049, Madrid, Spain
    J Biol Chem 274:16846-52. 1999
    ....
  95. pmc A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C
    Jonathan D Violin
    Department of Pharmacology, University of California, San Diego, La Jolla 92093, USA
    J Cell Biol 161:899-909. 2003
    ..Thus, specificity of PKC signaling depends on the local second messenger-controlled equilibrium between kinase and phosphatase activities to result in strict calcium-controlled temporal regulation of substrate phosphorylation...
  96. pmc Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
    Biol Cell 100:633-43. 2008
    ..Fertilization-mediated increase in PKC activity has been demonstrated; however, its precise role is unclear...
  97. pmc The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain
    F Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    Biochem J 318:583-90. 1996
    ..We therefore suggest that DGK does not contain a typical P-loop of ATP binding sites...
  98. ncbi Effect of diacylglycerol on the development of impaired glucose tolerance in sucrose-fed rats
    Shinichi Meguro
    Biological Science Laboratories, Kao Corporation, Tochigi, Japan
    Lipids 41:347-55. 2006
    ..In conclusion, these results suggest that DAG oil ingestion prevents the high-sucrose-diet-induced development of impaired glucose tolerance compared with TAG oil ingestion...
  99. ncbi Suppression of phospholipase C beta, gamma, and delta families alters cell growth and phosphatidylinositol 4,5-bisphosphate levels
    C G Nebigil
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Building 37, Room 1E24, Bethesda, Maryland 20892, USA
    Biochemistry 36:15949-58. 1997
    ....
  100. ncbi Targeting protein kinase C and "non-kinase" phorbol ester receptors: emerging concepts and therapeutic implications
    Marcelo G Kazanietz
    Department of Pharmacology, University of Pennsylvania School of Medicine, 816 Biomedical Research Building II III, 421 Curie Blvd, Philadelphia, PA 19104 6160, USA
    Biochim Biophys Acta 1754:296-304. 2005
    ..The discovery of the chimaerins and other "non-kinase" phorbol ester receptors has major implications in the design of agents for cancer therapy...
  101. ncbi MuLK, a eukaryotic multi-substrate lipid kinase
    David W Waggoner
    Department of Pharmacology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:38228-35. 2004
    ..The effects of charged lipids and cations on MuLK activity in vitro suggest that its activity in vivo is tightly regulated by cellular conditions...

Research Grants62

  1. Ether Linked Lipids and Signaling in Breast Tumor Cells
    ROBERT WYKLE; Fiscal Year: 2004
    ..tumor cells are known to contain phospholipase D that can be activated to convert PC to phosphatidic acid and diglycerides. PAF is known to promote cell growth and angiogenesis, whereas lyso PA promotes cell growth and tumor cell ..
  2. CD36 and Intestinal Fat Absorption
    Nada A Abumrad; Fiscal Year: 2013
    ..We found that CD36 is ubiquitinated on two C- terminal lysines, that ubiquitination is induced by FA and diglycerides and that it occurs during lipid absorption...
  3. METABOLISM OF PLATELET ACTIVATING FACTOR
    ROBERT WYKLE; Fiscal Year: 1993
    ..The role of diglycerides acid protein kinases will be examined in intact cells and cell-free systems...
  4. PLASMALOGEN CATABOLISM DURNIG MYOCARDIAL ISCHEMIA
    David Ford; Fiscal Year: 1993
    ..isoforms of protein kinase C and identification of isoforms which are selectively activated by ether-linked diglycerides. Specific alterations in membrane molecular dynamics and ion transport kinetics induced by plasmalogen ..
  5. PRIMING & STIMULATION OF GRANULOCYTES
    David Bass; Fiscal Year: 1992
    ..Priming and primed stimulation are hypothesized to involve interactions between 1) diglycerides, 2) arachidonic acid (AA) release and metabolism, 3) altered membrane structure, 4) synergy between DGs and/or ..
  6. TUMOR PROMOTER INDUCED PROTEIN PHOSPHORYLATION
    Eileen Friedman; Fiscal Year: 1992
    Transformation of human colonic epithelial cells confers on them sensitivity to new mitogenic signals. Fecal diglycerides (DGs), composed of oleic, myristic, or palmitic acid residues, were found in vivo at uM to mM concentrations (Cancer ..
  7. ETHER-LINKED DIGLYCERIDES AND PROTEIN KINASE C ISOTYPES
    Mark Kester; Fiscal Year: 2004
    ..Using in vivo and in vitro model systems, we have demonstrated that interleukin-1-generated ether-linked diglycerides (ether-DG) inhibit smooth muscle cell mitogenesis...
  8. PROTEIN KINASE C AND PHOTORECEPTORS
    ALEXANDRA NEWTON; Fiscal Year: 2002
    ..Specifically, the effect of this phosphorylation on rhodopsin s macromolecular interactions (transducin coupling, arrestin binding) will be explored. ..
  9. Regulation and function of the PKC/PKD signaling pathway
    Alex Toker; Fiscal Year: 2008
    ..A better understanding of the signaling pathways activated in response to ROS holds the potential for the development of novel therapeutic interventions for these pathologies. ..
  10. FASEB Summer Research Conference on Lipid Signaling Pathways in Cancer
    Alex Toker; Fiscal Year: 2007
    ..Emphasis will also be placed on the translational aspects of this field to provide the audience with an up-to-date view of the clinical implications of lipid signaling. [unreadable] [unreadable] [unreadable] [unreadable]..
  11. Structure and Activation of Protein Kinase C Enzymes
    JULIANNE SANDO; Fiscal Year: 2006
    ..If time permits, examination of additional isozymes will be initiated. ..
  12. REGULATION OF PHOSPHOLIPID SYNTHESIS
    Suzanne Jackowski; Fiscal Year: 2006
    ..The functions of the CCT regulatory domains characterized in vitro will also be evaluated for cellular localization and membrane binding in living cells. ..
  13. The Fourth International Leukocyte Signal Transduction Workshop: Clinical Implica
    Amnon Altman; Fiscal Year: 2007
    ..abstract_text> ..
  14. Topical Treatment for Mild Psoriasis
    Wendy Bollag; Fiscal Year: 2007
    ..An effective, easy-to-use, low-cost therapy suitable for long-term relief would be a great boon. [unreadable] [unreadable] [unreadable] [unreadable]..
  15. PKC modulation of calcium current and anesthetic action
    GANESAN KAMATCHI; Fiscal Year: 2007
    ..abstract_text> ..
  16. SALIVARY MUCOUS CELL GENE EXPRESSION
    DAVID CULP; Fiscal Year: 2007
    ..Moreover, expression of a novel human gene expressing a salivary apomucin will be verified, and if expressed, would be the focus of future studies to determine mechanisms regulating its expression. [unreadable] [unreadable]..
  17. Fat Induced Insulin Resistance and Atherosclerosis
    Guenther Boden; Fiscal Year: 2007
    ..We believe that these studies will provide important new information relative to the mechanism by which obesity and FFAs cause IR and ASVD in human subjects. ..
  18. Heterogeneity of hypoxic Ca2+ release in pulmonary and *
    Yong Xiao Wang; Fiscal Year: 2006
    ..The proposed studies are also of fundamental physiological significance with respect to the regulation of Ca2+ release in other cell types. ..
  19. Free Fatty Acids and Hepatic Insulin Resistance
    Guenther Boden; Fiscal Year: 2007
    ..These studies will hopefully provide much needed information relative to important details and mechanisms of FFA induced hepatic insulin resistance. [unreadable] [unreadable]..
  20. Novel Signaling for Ca2+ and Release in Airway Myocytes
    Yong Xiao Wang; Fiscal Year: 2007
    ..6, and/or RyRs are ubiquitously expressed in a variety of cell types, the mechanistic findings from the proposed study will have general biological and pathological significance for various Ca2+-mediated cellular effects. ..
  21. Role of Metalloproteinases in Colon Cancer Metastases
    DAVID GORDEN; Fiscal Year: 2008
    ..It is expected that during the period of this award, that the grantee would progress towards his objective of becoming an independent scientific investigator. [unreadable] [unreadable]..
  22. Protein kinase Dnu in regulated GLUT4 trafficking
    Qiming Wang; Fiscal Year: 2008
    ..Aim 3. To test the hypothesis that the PKC/PKDnu/VAMP2 signaling pathway contributes to phorbol ester and insulin-regulated recruitment of GLUT4 to the plasma membrane and glucose uptake. ..
  23. Cellular Physiology of Podocytes
    Stuart E Dryer; Fiscal Year: 2010
    ..Practically nothing is currently known about the effects of insulin on podocytes, which limits what we can know about diabetic nephropathy, especially in its earliest stages. ..
  24. Ichthyosis in Sjogren-Larsson Syndrome
    William B Rizzo; Fiscal Year: 2010
    ....
  25. Molecular Mechanism of Protein Kinase Cbeta-Mediated Cholesterol Homeostasis
    Kamal D Mehta; Fiscal Year: 2010
    ..The greatest potential for arresting this epidemic is likely to come through a clearer understanding of its molecular pathogenesis to develop rational and mechanism based therapy. ..
  26. Microvascular Regulation During Intestinal Absorption
    HAROLD G BOHLEN; Fiscal Year: 2010
    ..A substantial fraction of the NO in blood may be from NO loading in the small arteries based on their very high wall NO concentration and represents a new form of vascular regulation. ..
  27. Oral Infectious Disease: Virulence and Host Determinants
    DAVID CULP; Fiscal Year: 2009
    ..Future studies can then focus on molecular mechanisms of host-pathogen interactions. Results may indicate important therapeutic targets to treat patients at high risk for caries (i.e., patients with xerostomia). ..
  28. Role of Vav family proteins in cell signaling and cancer
    XOSE BUSTELO; Fiscal Year: 2009
    ..abstract_text> ..
  29. Gender differences in the interactions between endocannabinoids and stress
    Cecilia Hillard; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  30. Multiphoton Microscopy Of Early Tumor/Stromal Interactions In Liver Metastasis
    DAVID GORDEN; Fiscal Year: 2008
    ..A better understanding of the initiating events in metastasis will allow for improved targeted therapies for late stage cancers. [unreadable] [unreadable]..
  31. Role of PPARgamma in ultraviolet stress responses
    Raymond Konger; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  32. PHOSPHOLIPASE D AND KERATINOCYTE DIFFERENTIATION
    Wendy Bollag; Fiscal Year: 2007
    ..abstract_text> ..
  33. NOVEL REGULATORY MECHANISMS CONTROLLING BONE REPAIR AND OSTEOPOROSIS
    Peter Friedman; Fiscal Year: 2008
    ..The long-term objective is to identify novel compounds that can be used to treat post-menopausal osteoporosis. [unreadable] [unreadable] [unreadable]..
  34. EGF, Oxidants & Intestinal Barrier Integrity
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  35. Encoding Immune System Signals by the Mammalian Nose
    Frank Zufall; Fiscal Year: 2006
    ..A potential application of this reseach is in the development of olfactory sensors that can identify an individual based on genetically-determined odors. [unreadable] [unreadable]..
  36. Circadian Regulation of Olfaction
    STUART DRYER; Fiscal Year: 2004
    ..abstract_text> ..
  37. ANNEXIN PHOSPHORYLATION: CAUSES AND CONSEQUENCES
    CARL CREUTZ; Fiscal Year: 2004
    ....
  38. PKC theta in the Immune Synapse and T Cell Anergy
    Amnon Altman; Fiscal Year: 2003
    ..They offer the potential for developing novel therapeutic strategies, which may be useful for modulating CD28 costimulation in disease. ..
  39. ATHEROGENICITY OF APOB CONTAINING LIPOPROTEINS
    Ira Goldberg; Fiscal Year: 2003
    ..Moreover, the experiments are significant for our understanding of atherosclerosis development and may demonstrate molecular methods to alter apoB retention that could be used in treatment and preventative therapies. ..
  40. PLASMALOGEN CATABOLISM DURING MYOCARDIAL ISCHEMIA
    David Ford; Fiscal Year: 2003
    ..Specific Aim 3 is to test the hypothesis that myocardial ischemia/reperfusion injury is mediated, in part, by plasmalogen degradation by reactive chlorinating species released from activated neutrophils. ..
  41. FAT MEDIATED INSULIN SECRETION AND DIABETES IN ELDERLY
    Guenther Boden; Fiscal Year: 2003
    ..We hope that this project will help to better understand the mechanisms by which obesity contributes to the development of NIDDM. ..
  42. PKC Theta--A Novel Drug Target in T Cell Leukemias
    Amnon Altman; Fiscal Year: 2003
    ..abstract_text> ..
  43. MICROVASCULAR CHARACTERISTICS OF DIABETES MELLITUS
    HAROLD BOHLEN; Fiscal Year: 2002
    ..Positive results will confirm that chronic insulin resistance predisposes the microvasculature to abnormalities of endothelial function. However, antioxidants and inhibition of beta II isoenzymes of PKC may protect endothelial function. ..
  44. CALCIUM DEPENDENT MEMBRANE BINDING PROTEINS
    CARL CREUTZ; Fiscal Year: 2002
    ....
  45. EXERCISE AND SKELETAL MUSCLE FATTY ACID METABOLISM
    LORRAINE TURCOTTE; Fiscal Year: 2002
    ..For the first time, malonyl-CoA levels will be correlated to the rate of fatty acid oxidation in skeletal muscle. ..
  46. REGULATION OF GLANDULAR MUCOUS CELL SECRETION
    DAVID CULP; Fiscal Year: 2001
    ..Results will provide specific information for determination of the dose and temporal administration of agonist in future clinical trials to treat patients with salivary hypofunction. ..
  47. TSQ Quantum ESI-MS Surveyor Bundle
    David Ford; Fiscal Year: 2004
    ..This requested instrumentation will remedy this deficit in our otherwise well-equipped facility. ..
  48. Mechanisms of p42/44MAPK-induced LDL receptor expression
    KAMAL MEHTA; Fiscal Year: 2004
    ..This knowledge could be exploited to develop improved hypercholesterolemia therapies and to reduce foam cell formation. ..
  49. ROLE OF PGE2 RECEPTORS EP2 AND EP3 ON SENESCENCE
    Raymond Konger; Fiscal Year: 2004
    ..These studies will underscore how alterations of receptor expression serve to define the role of eicasonoids in diverse cellular functions. ..
  50. Regularity Mechanisms of Aldosterone Secretion
    Wendy Bollag; Fiscal Year: 2006
    ..The complex interactions between AngII and serum K+ levels suggest that abnormalities in the glomerulosa cell response to either agonist, or both, may contribute to the development of some forms of hypertension. [unreadable] [unreadable]..
  51. Protective Mechanism of an Anti-Inflammatory Herbal Mix
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  52. Cannabinoid Receptors and Cerebrovascular Function
    Cecilia Hillard; Fiscal Year: 2006
    ..Increased understanding of the mediators that are released by activated microglia and their targets may provide new therapeutic approaches to CNS diseases with an inflammatory component. ..
  53. MEMBRANE MECHANISMS OF OLFACTORY TRANSDUCTION
    Frank Zufall; Fiscal Year: 2006
    ..abstract_text> ..
  54. Hyperglycemia, Aldose Reducatse & Murine Atherosclerosis
    Ira Goldberg; Fiscal Year: 2005
    ..This is significant because it will provide for a model to study this complication and suggest a therapeutic target for prevention of diabetic macrovascular disease. ..
  55. CIRCADIAN REGULATION OF VERTEBRATE PHOTORECEPTORS
    STUART DRYER; Fiscal Year: 2005
    ..abstract_text> ..
  56. Gordon Research Conference on Calcium Signaling
    Colin Taylor; Fiscal Year: 2005
    ..abstract_text> ..
  57. Brain Endocannabinoids and Chronic Stress
    Cecilia Hillard; Fiscal Year: 2005
    ..The successful completion of these studies will enhance our understanding of the processes involved in the adaptation of the brain to chronic stress. ..
  58. TNF Alters Renal Tubular Function in Diabetes
    Frank Gesek; Fiscal Year: 2005
    ..Complementary techniques are used to examine alterations in vivo and at the cellular level. Our studies provide insights into the earliest changes that occur in DT due to the cytokine TNF. ..
  59. FUNCTION AND REGULATION OF VAV IN LYMPHOCYTE ACTIVATION
    Amnon Altman; Fiscal Year: 2005
    ....
  60. DEVELOPMENT OF IONIC CHANNELS
    STUART DRYER; Fiscal Year: 2005
    ..Neurotrophic factors are potential therapeutic agents for neurodegeneration. This research will provide additional information on neurotrophic factor actions and interactions, especially with respect to posttranslational effects. ..
  61. MEMBRANE MECHANISMS OF OLFACTORY TRANSDUCTION
    Frank Zufall; Fiscal Year: 2001
    ....