fluorescein 5 isothiocyanate

Summary

Summary: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Top Publications

  1. ncbi Activation of lysosomal function during dendritic cell maturation
    E Sergio Trombetta
    Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
    Science 299:1400-3. 2003
  2. ncbi Transfer of antigen between dendritic cells in the stimulation of primary T cell proliferation
    S C Knight
    Imperial College School of Medicine, Northwick Park Institute for Medical Research, Harrow, GB
    Eur J Immunol 28:1636-44. 1998
  3. ncbi ATP depletion inhibits the endocytosis of ClC-2
    Sonja U Dhani
    Programme in Molecular Structure and Function, Research Institute in the Hospital for Sick Children, Toronto, Canada
    J Cell Physiol 214:273-80. 2008
  4. ncbi Dendritic cell migration controlled by alpha 1b-adrenergic receptors
    G J Maestroni
    Center for Experimental Pathology, Istituto Cantonale di Patologia, Locarno, Switzerland
    J Immunol 165:6743-7. 2000
  5. ncbi Cytopathic and non-cytopathic bovine viral diarrhoea virus biotypes affect fluid phase uptake and mannose receptor-mediated endocytosis in bovine monocytes
    Bobbie L Boyd
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, PO Box 6100, 39762 6100, USA
    Vet Immunol Immunopathol 102:53-65. 2004
  6. ncbi Cubilin- and megalin-mediated uptake of albumin in cultured proximal tubule cells of opossum kidney
    X Y Zhai
    Department of Cell Biology, Institute of Anatomy and Department of Medical Biochemistry, University of Aarhus, Aarhus, Denmark
    Kidney Int 58:1523-33. 2000
  7. ncbi The dendritic cell populations of mouse lymph nodes
    S Henri
    Walter and Eliza Hall Institute of Medical Research, P O Royal Melbourne Hospital, Victoria 3050, Australia
    J Immunol 167:741-8. 2001
  8. ncbi Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
    Ricardo Pinto
    Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Biotechnol Prog 23:1492-7. 2007
  9. ncbi Preparation and in vitro evaluation of thiolated chitosan microparticles
    K Maculotti
    Department of Pharmaceutical Chemistry, University of Pavia, Italy
    J Microencapsul 22:459-70. 2005
  10. ncbi Different temperature sensitivity of endosomes involved in transport to lysosomes and transcytosis in rat hepatocytes: analysis by free-flow electrophoresis
    Isabella Ellinger
    Department of Pathophysiology, University of Vienna, Vienna, Austria
    Electrophoresis 23:2117-29. 2002

Research Grants

  1. Micellar VIP Nanoparticles for Rheumatoid Arthritis
    Israel Rubinstein; Fiscal Year: 2007
  2. PGE2 Regulation of Epithelial/Fibroblast Interactions
    Bethany Moore; Fiscal Year: 2006
  3. CXC CHEMOKINE REGULATION OF PROSTATE CANCER ANGIOGENESIS
    Bethany Moore; Fiscal Year: 2002
  4. Cerebral Microcirculation During Alcohol Consumption
    WILLIAM MAYHAN; Fiscal Year: 2008
  5. PERITONEAL DELIVERY OF ANTINEOPLASTIC MACROMOLECULES
    MICHAEL FLESSNER; Fiscal Year: 2004
  6. Effects of Hyaluronan on Interstitial Convection
    MICHAEL FLESSNER; Fiscal Year: 2007
  7. NICOTINE INDUCED CEREBROVASCULAR DYSFUNCTION
    WILLIAM MAYHAN; Fiscal Year: 2006
  8. Cerebrovascular Disease in Type 1 Diabetes
    WILLIAM MAYHAN; Fiscal Year: 2007
  9. DEFINING INNATE IMMUNE RECEPTOR LIGAND BINDING SITES
    Peter Tobias; Fiscal Year: 2007
  10. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006

Detail Information

Publications231 found, 100 shown here

  1. ncbi Activation of lysosomal function during dendritic cell maturation
    E Sergio Trombetta
    Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
    Science 299:1400-3. 2003
    ..Lysosomal function in DCs thus appears to be specialized for the developmentally regulated processing of internalized antigens...
  2. ncbi Transfer of antigen between dendritic cells in the stimulation of primary T cell proliferation
    S C Knight
    Imperial College School of Medicine, Northwick Park Institute for Medical Research, Harrow, GB
    Eur J Immunol 28:1636-44. 1998
    ..We propose that this unique interaction between antigen-presenting cells may be a paradigm for self/non-self discrimination...
  3. ncbi ATP depletion inhibits the endocytosis of ClC-2
    Sonja U Dhani
    Programme in Molecular Structure and Function, Research Institute in the Hospital for Sick Children, Toronto, Canada
    J Cell Physiol 214:273-80. 2008
    ..In summary, we have determined that the internalization of ClC-2 by endocytosis is inhibited by metabolic stress, highlighting the importance for understanding the molecular mechanisms mediating the endosomal trafficking of this channel...
  4. ncbi Dendritic cell migration controlled by alpha 1b-adrenergic receptors
    G J Maestroni
    Center for Experimental Pathology, Istituto Cantonale di Patologia, Locarno, Switzerland
    J Immunol 165:6743-7. 2000
    ..This finding might improve our understanding of the biological basis of skin diseases and allergic reactions, and opens new pharmacological possibilities to modulate the immune response...
  5. ncbi Cytopathic and non-cytopathic bovine viral diarrhoea virus biotypes affect fluid phase uptake and mannose receptor-mediated endocytosis in bovine monocytes
    Bobbie L Boyd
    Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, PO Box 6100, 39762 6100, USA
    Vet Immunol Immunopathol 102:53-65. 2004
    ..By influencing the early antigen uptake function of APC, BVDV might disrupt the function of monocytes as professional APC and contribute to the specific immunotolerance to BVDV...
  6. ncbi Cubilin- and megalin-mediated uptake of albumin in cultured proximal tubule cells of opossum kidney
    X Y Zhai
    Department of Cell Biology, Institute of Anatomy and Department of Medical Biochemistry, University of Aarhus, Aarhus, Denmark
    Kidney Int 58:1523-33. 2000
    ..Two giant glycoproteins, cubilin and megalin, constitute important endocytic receptors localized to the kidney proximal tubule...
  7. ncbi The dendritic cell populations of mouse lymph nodes
    S Henri
    Walter and Eliza Hall Institute of Medical Research, P O Royal Melbourne Hospital, Victoria 3050, Australia
    J Immunol 167:741-8. 2001
    ..The putative LC-derived DC among skin emigrants and in LN also showed strong intracellular staining of langerin...
  8. ncbi Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibers
    Ricardo Pinto
    Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Biotechnol Prog 23:1492-7. 2007
    ..By observing different spots in the surface of the fibers, site-specific information is obtained. It was verified that the physically heterogeneous fibers exhibit different amounts of adsorbed CBDs...
  9. ncbi Preparation and in vitro evaluation of thiolated chitosan microparticles
    K Maculotti
    Department of Pharmaceutical Chemistry, University of Pavia, Italy
    J Microencapsul 22:459-70. 2005
    ..5-fold higher concentration with respect to unmodified chitosan microparticles. These data suggest that chitosan-TBA conjugate microspheres have the potential to be used as a mucoadhesive drug delivery system...
  10. ncbi Different temperature sensitivity of endosomes involved in transport to lysosomes and transcytosis in rat hepatocytes: analysis by free-flow electrophoresis
    Isabella Ellinger
    Department of Pathophysiology, University of Vienna, Vienna, Austria
    Electrophoresis 23:2117-29. 2002
    ....
  11. ncbi Biliary secretion of fluid phase markers is modified under post-cholestatic conditions
    Isabella Ellinger
    Department of Pathophysiology, Medical University of Vienna, Vienna, Austria
    Wien Med Wochenschr 158:579-82. 2008
    ..This fast route is taken only by HRP but not by FITC-dextran, the latter being exclusively transported by a transcellular route under all conditions investigated...
  12. ncbi Enhanced epidermal Langerhans cell migration in IL-10 knockout mice
    B Wang
    Division of Dermatology, Sunnybrook Health Science Center, University of Toronto, Ontario, Canada
    J Immunol 162:277-83. 1999
    ..These data suggest that IL-10 plays an inhibitory role in LC migration and that this effect may occur via the down-regulation of TNF-alpha and IL-1 production...
  13. ncbi Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructure
    Lars Hildén
    WURC, Department of Wood Science, Swedish University of Agricultural Sciences, Box 7008, 750 07 Uppsala, Sweden
    Biotechnol Lett 25:553-8. 2003
    ..The labelled CBM was successfully used as a probe for detecting cellulose in lignocellulose material such as never dried spruce and birch wood as well as pulp fibres...
  14. ncbi Endosomal alkalinization reduces Jmax and Km of albumin receptor-mediated endocytosis in OK cells
    M Gekle
    Department of Physiology, , Germany
    Am J Physiol 268:F899-906. 1995
    ..Endosomal alkalinization disturbs intracellular ligand handling and receptor trafficking, leading to a reduction of endocytotic capacity and affinity...
  15. ncbi Extravascular transport of fluorescently labeled albumins in the rat mesentery
    Norman R Harris
    Department of Bioengineering, Pennsylvania State University, University Park, PA 16802 6804, USA
    Microcirculation 9:177-87. 2002
    ....
  16. ncbi A novel tool to characterize paracellular transport: the APTS-dextran ladder
    Winfried Neuhaus
    Department of Medicinal Chemistry, University of Vienna, Pharmacy Center, Althanstrasse 14, A 1090, Vienna, Austria
    Pharm Res 23:1491-501. 2006
    ..The aim of this work was to develop an easy, manageable, and precise analytic tool to describe the tightness of cell layers by a molecular weight ladder...
  17. ncbi The uptake of Texas Red-BSA in the excretory system of schistosomes and its colocalisation with ER60 promoter-induced GFP in transiently transformed adult males
    Volker Wippersteg
    Institute for Genetics, Genetic Parasitology and Center for Biological and Medical Research, Heinrich Heine University, Universitatsstrasse 1, D 40225, Dusseldorf, Germany
    Int J Parasitol 33:1139-43. 2003
    ..The results suggest a role for ER60 during the host-parasite interaction. Furthermore, the colocalisation approach introduced here opens further perspectives to characterise gene-expression profiles in this parasite...
  18. ncbi Comparison of the endocytic properties of linear and branched PEIs, and cationic PAMAM dendrimers in B16f10 melanoma cells
    F Philipp Seib
    Centre for Polymer Therapeutics, Welsh School of Pharmacy, King Edward VII Avenue, Cardiff University, Cardiff CF10 3XF, UK
    J Control Release 117:291-300. 2007
    ..A perception of the rate and mechanisms of cellular uptake of these vectors will be important in the context of their proposed use as drug delivery systems...
  19. pmc Chloride-dependent secretion of alveolar wall liquid determined by optical-sectioning microscopy
    Jens Lindert
    Lung Biology Laboratory, Department of Physiology and Cellular Biophysics, Columbia University, College of Physicians and Surgeons, SLRHC, New York, New York, USA
    Am J Respir Cell Mol Biol 36:688-96. 2007
    ..We conclude that CFTR-dependent liquid secretion is present in alveoli of the adult mouse. Defective alveolar secretion might impair alveolar immune defense and promote alveolar disease...
  20. ncbi Prevention of chronic graft-versus-host disease by stimulation with glucocorticoid-induced TNF receptor
    Juyang Kim
    The Immunomodulation Research Center and Department of Biological Science, University of Ulsan, Ulsan 680 749, Korea
    Exp Mol Med 38:94-9. 2006
    ..Our results indicate that anti-GITR monoclonal antibody may be used as a potential immunotherapeutic agent for preventing cGVHD...
  21. ncbi Rapid determination of renal filtration function using an optical ratiometric imaging approach
    Weiming Yu
    Indiana University School of Medicine, Department of Medicine, Nephrology Division, Indianapolis, IN 46202, USA
    Am J Physiol Renal Physiol 292:F1873-80. 2007
    ..This approach also offers a high sensitivity for determining plasma clearance rate of a fluorescent compound. This feature is important for rapidly quantifying small differences in plasma clearance when kidney function is changing...
  22. ncbi A rabbit model for monitoring in vivo viability of human platelet concentrates using flow cytometry
    Valery Leytin
    Department of Transfusion Medicine, St Michael s Hospital, Toronto, Ontario, Canada
    Transfusion 42:711-8. 2002
    ..Viability in vivo of novel platelet components cannot be readily determined in human transfusions. Elaboration of valid animal models may be useful for this purpose...
  23. ncbi Layer-by-layer coating of degradable microgels for pulsed drug delivery
    B G De Geest
    Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
    J Control Release 116:159-69. 2006
    ..Further, we show that the application of an LbL coating on the surface of the microgels dramatically lowers the burst release from the microcapsules and results in massive release at the time the microcapsules rupture...
  24. ncbi Localization of the permeability barrier to solutes in isolated arteries by confocal microscopy
    Paul M A van Haaren
    Department of Medical Physics, Cardiovascular Research Institute Amsterdam, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
    Am J Physiol Heart Circ Physiol 285:H2848-56. 2003
    ..This study demonstrates the presence of a thick ESL that contributes to the permeability barrier to solutes. The layer is sensitive to phototoxic stress, and its damage could form an early event in atherosclerosis...
  25. ncbi Nanoparticles based on N-trimethylchitosan: evaluation of absorption properties using in vitro (Caco-2 cells) and ex vivo (excised rat jejunum) models
    Giuseppina Sandri
    Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy
    Eur J Pharm Biopharm 65:68-77. 2007
    ..The improvement of mucoadhesion and of nanoparticle internalisation with respect to chitosan nanosystems makes the TMCs nanosystems suitable carriers for the intestinal absorption of peptides...
  26. ncbi Effects of mannosylated glycopolymers on specific interaction to bone marrow hematopoietic and progenitor cells derived from murine species
    Keun Hong Park
    College of Medicine, CHA Stem Cell Institute 606 16, Pochon CHA University, Yeoksam 1 dong, Kangnam Gu, Seoul 135 081, Republic of Korea
    J Biomed Mater Res A 82:281-7. 2007
    ....
  27. ncbi Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells
    K J de Vries
    Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    Biol Reprod 68:2122-34. 2003
    ..The proportion of live cells with PL scrambling corresponded with that showing capacitation-specific chlortetracyclin staining...
  28. ncbi Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperature
    Günther Baravalle
    Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Wahringer Gurtel 18 20, 1090, Vienna, Austria
    Cell Tissue Res 320:99-113. 2005
    ..Consequently, these treatments can be applied to investigate whether internalized macromolecules such as viruses follow a recycling or degradative pathway...
  29. ncbi Isoprostane 8-epi-prostaglandin F2 alpha decreases lymph capillary pressure in patients with primary lymphedema
    Beatrice R Amann-Vesti
    Department of Medicine, Angiology Division, University Hospital, Zurich, Switzerland
    J Vasc Res 40:77-82. 2003
    ..0 +/- 7.7 mm Hg). In healthy volunteers, there was no significant decrease of LCP with PGF compared to placebo. PGF normalizes microlymphatic hypertension in patients with lymphedema by improving lymph transport into deeper channels...
  30. ncbi The use of quantum dots for analysis of chick CAM vasculature
    Jason D Smith
    Institute for Complex Engineered Systems, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA
    Microvasc Res 73:75-83. 2007
    ..We also demonstrated that QDs are fixable with low fluorescence loss and thus can be used in conjunction with histological processing for further sample analysis...
  31. ncbi A smart membrane based on an antigen-responsive hydrogel
    Rongsheng Zhang
    Department of Chemical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Biotechnol Bioeng 97:976-84. 2007
    ..Sequential addition and removal of sodium fluorescein showed that these permeability changes are reversible...
  32. ncbi Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routes
    Serge Camelo
    School of Anatomy and Human Biology, The University of Western Australia, Crawley Perth
    Invest Ophthalmol Vis Sci 47:1039-46. 2006
    ..To determine the afferent pathways linking the anterior chamber (AC) of the eye to the secondary lymphoid organs...
  33. pmc DEC-205lo Langerinlo neonatal Langerhans' cells preferentially utilize a wortmannin-sensitive, fluid-phase pathway to internalize exogenous antigen
    Bernadette M Bellette
    Discipline of Pathology, University of Tasmania, Hobart, Australia
    Immunology 110:466-73. 2003
    ..As neonatal LC are capable of sampling their environment without inducing immunity, this may serve to avoid inappropriate immune responses during the neonatal period...
  34. ncbi CCL5-CCR5 interaction provides antiapoptotic signals for macrophage survival during viral infection
    Jeffrey W Tyner
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Med 11:1180-7. 2005
    ..The antiapoptotic action of chemokine signaling may therefore allow scavengers to finally stop the host cell-to-cell infectious process...
  35. pmc Uptake of host cell transforming growth factor-beta by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion
    Mariana C Waghabi
    Laboratorios de Biologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
    Am J Pathol 167:993-1003. 2005
    ..These observations represent the first example of a novel mechanism by which a primitive unicellular protozoan can use host cell TGF-beta to control its own intracellular life cycle...
  36. ncbi Transforming growth factor-beta-dependent growth inhibition in primary vascular smooth muscle cells is p38-dependent
    Ulrike Seay
    University of Giessen Lung Center, Department of Medicine I, Aulweg 123, Room 6 11, D 35392 Giessen, Germany
    J Pharmacol Exp Ther 315:1005-12. 2005
    ..The anti-proliferative effect of TGF-beta is due to G0/G1 arrest and mediated primarily by the p38 pathway, suggesting that p38 kinase is central to TGF-beta-mediated growth inhibition in primary mouse VSMCs...
  37. ncbi The impact of topical antiseptics on skin microcirculation
    S Langer
    Department of Plastic and Hand Surgery, Burn Center, BG University Hospital, Ruhr Universitat Bochum, Buerkle de la Camp Platz 1, D 44789 Bochum, Germany
    Eur J Med Res 9:449-54. 2004
    ..However, topical antiseptics are assumed to have an influence on skin microcirculation, per se. Thus, the aim of the study was to analyse the influence of topically applied antiseptics on the microcirculation of intact skin in vivo...
  38. ncbi Submucosal gland dysfunction as a primary defect in cystic fibrosis
    Danieli Salinas
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    FASEB J 19:431-3. 2005
    ..Our results suggest that defective airway submucosal gland function is an early, primary defect in CF. Therapies directed at normalizing gland fluid secretion early in CF may thus reduce lung disease...
  39. ncbi Effective permeability of hydrophilic substances through walls of lymph vessels: roles of endothelial barrier
    Nobuyuki Ono
    Department of Physiology, Shinshu University School of Medicine, 3 1 1 Asahi, Matsumoto, 390 8621 Japan
    Am J Physiol Heart Circ Physiol 289:H1676-82. 2005
    ....
  40. ncbi Synthesis, antitumor evaluation, and apoptosis-inducing activity of hydroxylated (E)-stilbenes
    Cedric J Lion
    Cancer Research U K Experimental Cancer Chemotherapy Research Group, Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, UK
    J Med Chem 48:1292-5. 2005
    ..g., 5dy and 5jy, an observation further reinforced by AnnexinV-FITC analysis and fluorescence microscopy...
  41. ncbi Farnesylated RhoB inhibits radiation-induced mitotic cell death and controls radiation-induced centrosome overduplication
    J Milia
    INSERM U563, CPTP, Département d Innovation Thérapeutique et d Oncologie Moléculaire, Institut Claudius Regaud, 20 24 rue du Pont St Pierre, 31052 Toulouse Cedex, France
    Cell Death Differ 12:492-501. 2005
    ..Overall, our results for the first time clearly implicate farnesylated RhoB as a crucial protein in mediating cellular resistance to radiation-induced nonapoptotic cell death...
  42. ncbi Temporary loss of plasma membrane integrity in orthodontic tooth movement
    M F Orellana-Lezcano
    Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada
    Orthod Craniofac Res 8:106-13. 2005
    ..These studies provide evidence of a novel cellular mechanism for uptake and release of molecules and suggest a potential role for plasma membrane disruption in the mechanotransduction of orthodontic tooth movement...
  43. pmc Antimalarial quinolines and artemisinin inhibit endocytosis in Plasmodium falciparum
    Heinrich C Hoppe
    Division of Pharmacology, University of Cape Town Medical School, Groote Schuur Hospital Old Building, Observatory, Cape Town 7925, South Africa
    Antimicrob Agents Chemother 48:2370-8. 2004
    ..These results are discussed in the light of present theories regarding the mechanisms of action of the antimalarials and highlight the potential use of drugs in manipulating and studying the endocytic pathway of malaria parasites...
  44. ncbi Critical effect of freezing/freeze-drying on sustained release of FITC-dextran encapsulated within PLGA microspheres
    Tae Hyoung Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, South Korea
    Int J Pharm 271:207-14. 2004
    ..Vacuum-drying was a good alternative choice in reducing the initial burst, compared to freeze-drying...
  45. ncbi Ciliary reorientation is evoked by a rise in calcium level over the entire cilium
    Yoshiaki Iwadate
    Department of Life Science, Faculty of Integrated Arts and Sciences, The University of Tokushima, Tokushima, Japan
    Cell Motil Cytoskeleton 57:197-206. 2004
    ..These results strongly suggest that not only Ca2+ influx sites, but also Ca2+ binding sites and vectoral bending machineries for ciliary reversal, are distributed throughout the cilium...
  46. ncbi Neural tissue engineering: a self-organizing collagen guidance conduit
    James B Phillips
    University College London, Tissue Repair and Engineering Centre, Institute of Orthopaedics and Musculoskeletal Science, Royal National Orthopaedic Hospital, Stanmore, Middlesex, UK
    Tissue Eng 11:1611-7. 2005
    ..Neural regeneration through this device was significantly greater than in controls, demonstrating that this system has potential both as a simple robust clinical implant and as a three-dimensional engineered tissue model...
  47. ncbi Use of a fluorescent membrane probe to identify zooxanthellae in hospite among dissociated endoderm cell culture from coral
    C S Chen
    Symbiosis Research Group, National Museum of Marine Biology and Aquarium, 2 Houwan Road, Checheng, Pingtung, 944 Taiwan, ROC
    Protoplasma 226:175-9. 2005
    ..This method has provided a fast and reliable assay to identify symbiotic endoderm cells and will greatly accelerate the progress of endosymbiosis research...
  48. ncbi Molecular characterization and immunohistochemical localization of palmdelphin, a cytosolic isoform of the paralemmin protein family implicated in membrane dynamics
    Bin Hu
    Institut fur Physiologische Chemie, Ruhr Universitat Bochum, Germany
    Eur J Cell Biol 84:853-66. 2005
    ..These observations suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures...
  49. ncbi Morphology and metabolism of hepatocytes microencapsulated with acrylic terpolymer-alginate using gelatin and poly(vinyl alcohol) as extracellular matrices
    J S Moon
    Department of Polymer Science, Kyungpook National University, Taegu 702 701, South Korea
    J Biomater Sci Polym Ed 16:1245-59. 2005
    ..The hepatocytes encapsulated in both alginate and acrylic terpolymer with gelatin and PVA rapidly aggregated in the core. The aggregated cells showed high albumin synthesis and ammonia removal, suggesting good metabolic function...
  50. ncbi The ApoCorrect assay: a novel, rapid method to determine the biological functionality of radiolabeled and fluorescent Annexin A5
    Hendrikus H Boersma
    Department of Clinical Pharmacy and Toxicology, University Hospital Maastricht, P O Box 5800, NL 6202 AZ Maastricht, The Netherlands
    Anal Biochem 327:126-34. 2004
    ..The bead assay is specific, stability-indicating, repeatable, and reproducible. It allows one to determine within 25 min the biological active fraction of a 99mTc-Annexin A5 preparation. We dubbed this assay the ApoCorrect assay...
  51. ncbi Pesticide mixtures potentiate the toxicity in murine thymocytes
    Selen Olgun
    Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Toxicology 196:181-95. 2004
    ..As more information is obtained on the potential immunotoxic effects of pesticides, further insights will be gained for the risk assessment of these environmental pollutants...
  52. ncbi Clustered carbohydrates as a target for natural killer cells: a model system
    Elena I Kovalenko
    Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ul Miklukho Maklaya, 16 10, Moscow 117997, Russia
    Histochem Cell Biol 127:313-26. 2007
    ..Only polymeric, or clustered, but not monomeric glycoconjugates resulted in alteration of cytotoxicity in our system, suggesting that appropriate presentation is critical for carbohydrate recognition and subsequent biological effects...
  53. ncbi Ca(2+) signalling and gap junction coupling within and between pigment epithelium and neural retina in the developing chick
    Rachael A Pearson
    Department of Physiology, University College London, Gower Street, London, WC1E 6BT
    Eur J Neurosci 19:2435-45. 2004
    ..We conclude that gap junctions between progenitor cells, and between these cells and the RPE, may orchestrate retinal proliferation/differentiation, via the propagation of Ca(2+) or other signalling molecules...
  54. ncbi Nanosecond pulsed electric fields (nsPEF) induce direct electric field effects and biological effects on human colon carcinoma cells
    Emily H Hall
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital of the King s Daughters, Norfolk, Virginia 23510, USA
    DNA Cell Biol 24:283-91. 2005
    ..NsPEF applications provide a means to explore intracellular structures and functions that can reveal mechanisms in health and disease...
  55. ncbi Progesterone stimulation of fluid absorption by the rat uterine gland
    R J Naftalin
    Division of Physiology, King s College London, New Hunt s House, Guys Campus, SE1 1UL, UK
    Reproduction 123:633-8. 2002
    ..This study provides the first direct evidence of fluid absorption by the uterine glands. Such absorption may provide the mechanism for closure of the uterine lumen and immobilization of the blastocyst necessary for implantation...
  56. ncbi Surface expression of complement receptor gC1q-R/p33 is increased on the plasma membrane of human spermatozoa after capacitation
    Kristen S Grace
    Department of Medicine, State University of New York, Stony Brook, New York 11794 8161, USA
    Biol Reprod 66:823-9. 2002
    ..In addition, the ability of live spermatozoa to bind to monoclonal anti-gC1qR antibody-coated microtiter wells was also increased after capacitation. These results suggest that gC1qR may play a role in human fertilization...
  57. ncbi Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo
    A C W Zannettino
    Myeloma Research Program, Bone and Cancer Laboratories, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Adelaide, Australia
    J Cell Physiol 214:413-21. 2008
    ..The findings presented here, describe a multipotential stem cell population within adult human adipose tissue, which appear to be intimately associated with perivascular cells surrounding the blood vessels...
  58. ncbi In vivo fluorescence measurement of Na(+) concentration in the pericryptal space of mouse descending colon
    J R Thiagarajah
    Division of Physiology, Centre for Vascular Biology and Medicine, King's College London, Guy's Campus, London SE1 1UL, United Kingdom
    Am J Physiol Cell Physiol 281:C1898-903. 2001
    ..The pericryptal space represents the first example of a hypertonic extracellular compartment in mammals that is not created by a countercurrent amplification mechanism...
  59. ncbi Geometric characteristics of arterial network of rat pial microcirculation
    D Lapi
    Department of Physiology and Biochemistry, University of Pisa, Pisa, Italy
    J Vasc Res 45:69-77. 2008
    ..The aim of the study was to assess the geometric characteristics of rat pial microcirculation and describe the vessel bifurcation patterns by 'connectivity matrix'...
  60. ncbi A miniature head-mounted two-photon microscope. high-resolution brain imaging in freely moving animals
    F Helmchen
    Biological Computation Research Department, Bell Laboratories, Lucent Technologies, Murray Hill, NJ 07974, USA
    Neuron 31:903-12. 2001
    ..Capillary blood flow and dendritic calcium transients were measured with high time resolution using line scans. In awake, freely moving rats, stable imaging was possible except during sudden head movements...
  61. pmc Characterization of protein factor(s) in rat bronchoalveolar lavage fluid that enhance insulin transport via transcytosis across primary rat alveolar epithelial cell monolayers
    Rana Bahhady
    Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90089 9121, USA
    Eur J Pharm Biopharm 69:808-16. 2008
    ....
  62. ncbi In vivo confocal laser laparoscopy allows real time subsurface microscopy in animal models of liver disease
    Martin Goetz
    I Medizinische Klinik und Poliklinik, Johannes Gutenberg Universitat Mainz, Germany
    J Hepatol 48:91-7. 2008
    ..Therefore a newly developed miniaturised confocal probe was evaluated for in vivo microscopic imaging in rodent models of human liver diseases...
  63. ncbi Acute effects of whole-body exposure to static magnetic fields and 50-Hz electromagnetic fields on muscle microcirculation in anesthetized mice
    S Xu
    Department of Physiological Hygiene, National Institute of Public Health, Tokyo, Japan
    Bioelectrochemistry 53:127-35. 2001
    ..These findings suggest that field intensity of 1 mT might be considered as a threshold level for enhancing muscle microcirculation under pentobarbital-induced hypnosis...
  64. ncbi The differential influence of non-iodinated and mono- or diiodinated benzoic acids on cellular and nuclear uptake of the nuclear localization sequence of the SV 40 T antigen
    Stefan Heckl
    Department of Neuroradiology, University of Tubingen, Germany
    Int J Pharm 355:131-40. 2008
    ..Such mono- or diiodobenzoic acid conjugates may therefore have potential in the development of new non-radioactive drugs against malignant glioma cells...
  65. ncbi Intravital microscopic analysis of vascular perfusion and macromolecule extravasation after photodynamic vascular targeting therapy
    Chong He
    Department of Pharmaceutical Sciences, Philadelphia College of Pharmacy, University of the Sciences in Philadelphia, 600 South 43rd Street, Philadelphia, Pennsylvania 19104, USA
    Pharm Res 25:1873-80. 2008
    ..Since tumor vasculature is the common route for the delivery of both blood and therapeutic agents, it is important to compare the effects of PDT on blood perfusion and substance transport...
  66. ncbi Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamber
    Wajeeh Saadi
    Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science II, Irvine, CA 92697, USA
    Biomed Microdevices 9:627-35. 2007
    ..The Ladder Chamber's simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels...
  67. ncbi Investigation of antigen delivery route in vivo and imune-boosting effects mediated by pH-sensitive liposomes encapsulated with K(b)-restricted CTL epitope
    Ki Young Lee
    Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, 134 Shinchon dong, Seodaemun gu, Seoul, 120 749, South Korea
    Biochem Biophys Res Commun 292:682-8. 2002
    ..These results suggest that pH-sensitive liposome formula functions as a potential adjuvant of peptide antigens and is useful for the induction of antigen specific CTLsv in vivo...
  68. ncbi A novel imaging technique for experimental choroidal neovascularization
    Mercedes Campos
    Biological Imaging Core, National Eye Institute, NIH, Bethesda, Maryland 20892 0703, USA
    Invest Ophthalmol Vis Sci 47:5163-70. 2006
    ..The authors developed an imaging technique for visualizing and quantifying morphologic changes associated with experimental laser-induced CNV...
  69. ncbi The BEACH protein LvsB is localized on lysosomes and postlysosomes and limits their fusion with early endosomes
    Elena Kypri
    Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
    Traffic 8:774-83. 2007
    ..These results support the model that LvsB and Lyst proteins act as negative regulators of fusion by limiting the heterotypic fusion of early endosomes with postlysosomal compartments...
  70. ncbi Combination of doxorubicin and low-intensity ultrasound causes a synergistic enhancement in cell killing and an additive enhancement in apoptosis induction in human lymphoma U937 cells
    Toru Yoshida
    Second Department of Surgery, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, 930 0194, Japan
    Cancer Chemother Pharmacol 61:559-67. 2008
    ..Here, the combined effects of low-intensity pulsed US and doxorubicin (DOX) on cell killing and apoptosis induction of U937 cells, and mechanisms involved were investigated...
  71. pmc NS-398, a cyclooxygenase-2-specific inhibitor, delays skeletal muscle healing by decreasing regeneration and promoting fibrosis
    Wei Shen
    Departments of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Am J Pathol 167:1105-17. 2005
    ..Our findings suggest that the use of cyclooxygenase-2-specific inhibitors to treat skeletal muscle injuries warrants caution because they may interfere with muscle healing...
  72. ncbi Inhibition of proliferation and apoptosis of vascular smooth muscle cells by ghrelin
    Min Zhan
    The Affiliated Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China
    Acta Biochim Biophys Sin (Shanghai) 40:769-76. 2008
    ..Furthermore, the former effect is probably mediated by the growth hormone secretagogue receptor type 1a receptor, while the latter effect may be mediated through other receptors...
  73. ncbi Intravital fluorescence microscopy and phototocicity: effects on leukocytes
    Anthony G Harris
    Institute for Surgical Research, Klinikum Grosshadern, Marchioninistr 15, D 81366 Munich, Germany
    Eur J Med Res 7:117-24. 2002
    ....
  74. ncbi Targeted inactivation of murine band 3 (AE1) gene produces a hypercoagulable state causing widespread thrombosis in vivo
    H Hassoun
    Department of Biomedical Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA, USA
    Blood 92:1785-92. 1998
    ....
  75. ncbi Determination of macromolecular exchange and PO2 in the microcirculation: a simple system for in vivo fluorescence and phosphorescence videomicroscopy
    L N Torres
    , , Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, RJ, Brasil
    Braz J Med Biol Res 34:129-35. 2001
    ..05 to 0.5 mg/ml). The system was successfully used to study macromolecular extravasation and PO2 in the rat mesentery circulation under controlled conditions and during ischemia-reperfusion...
  76. ncbi Fluorescence resonance energy transfer between points on tropomyosin and actin in skeletal muscle thin filaments: does tropomyosin move?
    M Miki
    Department of Applied Chemistry and Biotechnology, Fukui University, Fukui, 910 8507, Japan
    J Biochem 123:1104-11. 1998
    ..The present results do not support the notion of tropomyosin movement on skeletal muscle thin filaments as proposed in the steric blocking theory...
  77. ncbi A selective reaction of fructose bisphosphate aldolase with fluorescein isothiocyanate in chicken muscle extracts
    Andrew G Gehring
    United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    J Mol Recognit 21:137-47. 2008
    ..Unlike chicken and rabbit muscle aldolases, chicken brain and liver aldolase isoforms along with several other aldolases derived from diverse biological sources did not exhibit this highly selective modification by 5'-FITC...
  78. ncbi Identification of arginyl residues located at the ATP binding site of sarcoplasmic reticulum Ca2+-ATPase. Modification with 1,2-cyclohexanedione
    K Kimura
    Department of Biochemistry, Asahikawa Medical College, Asahikawa 078, Japan
    J Biol Chem 271:28933-41. 1996
    ..This suggests that the arginyl residues involved in these inhibitions are distinct from that involved in the inhibition of the Ca2+-ATPase activity...
  79. ncbi [Characteristics and in vitro imaging study of matrix metalloproteinase-2 targeting activable cell-penetrating peptide]
    Min Liu
    Imaging Center, The 2nd Affiliated Hospital of Medical School, Xi an Jiao Tong University, Xi an 710004, China
    Zhonghua Yi Xue Za Zhi 87:233-9. 2007
    ..To study invasively imaging MMP2-positive tumor cell by paramagnetic Gadolinium or fluorescein carried by a activable cell penetrating peptides...
  80. ncbi Ligands presumed to label high affinity and low affinity ATP binding sites do not interact in an (alpha beta)2 diprotomer in duck nasal gland Na+,K+-ATPase, nor Do the sites coexist in native enzyme
    D W Martin
    Division of Hematology, Department of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794 8151, USA
    J Biol Chem 275:24512-7. 2000
    ..Low affinity sites do not exist on the unmodified enzyme but can be detected only after the high affinity site is modified by FITC...
  81. ncbi Autonomous folding of the recombinant large cytoplasmic loop of sarcoplasmic reticulum Ca2+-ATPase probed by affinity labeling and trypsin digestion
    M J Moutin
    Laboratoire de Biophysique Moléculaire et Cellulaire, URA 520 CNRS, CEA, DBMS CENG, Grenoble, France
    Eur J Biochem 251:682-90. 1998
    ..The hinge region does not appear to be part of the FITC-binding site but constitutes portions of the 2',3'-O-(2,4,6-trinitrophenyl)-ATP and, probably, ATP-binding site...
  82. ncbi Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase
    Kazuhiro Abe
    Biological Chemistry, Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    J Biochem 138:293-301. 2005
    ....
  83. ncbi Sub-lethal photodynamic damage to ARPE-19 cells transiently inhibits their phagocytic activity
    Magdalena Olchawa
    Department of Biophysics, Jagiellonian University, Krakow, Poland
    Photochem Photobiol 86:772-80. 2010
    ..We postulate that oxidative damage to key components of the cell phagocytic machinery may be responsible for severe impairment of its activity, which can lead to retinal degeneration...
  84. ncbi Characterization of interactions involving anti-metatype antibodies and immune complexes
    K M Weidner
    Department of Microbiology, University of Illinois, Urbana 61801
    Mol Immunol 29:303-12. 1992
    ....
  85. ncbi Effects of a novel pathogenesis-related class 10 (PR-10) protein from Crotalaria pallida Roots with papain inhibitory activity against root-knot nematode Meloidogyne incognita
    Lúcia Betânia da S Andrade
    Centro de Ciencias Agrarias e Biologicas, Universidade Estadual Vale do Acarau UVA, Sobral, Ceara CE, Brazil
    J Agric Food Chem 58:4145-52. 2010
    ..In a localization study, fluorescein-5-isothiocyanate (FITC)-CpPRI was observed to internalize and diffuse over the entire J2 body after 6 h of incubation. This fact could explain the natural tolerance of this plant species to nematodes...
  86. ncbi Killing of Candida albicans by histatin 5: cellular uptake and energy requirement
    C Gyurko
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Massachusetts 02118 2392, USA
    Antonie Van Leeuwenhoek 79:297-309. 2001
    ..These results indicated that histatin 5 killing activity could be directly correlated to histatin 5 internalization. Both of these processes are prevented by modulators of cellular metabolic activity...
  87. ncbi Cellubrevin-targeted fluorescence uncovers heterogeneity in the recycling endosomes
    K Teter
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
    J Biol Chem 273:19625-33. 1998
    ..Based on the combination of these morphological and functional data, we suggest that the recycling endosomes are heterogeneous in their biochemical compositions, ion transport properties, and pH values...
  88. ncbi Damage of Escherichia coli membrane by bactericidal agent polyhexamethylene guanidine hydrochloride: micrographic evidences
    Z x Zhou
    State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China
    J Appl Microbiol 108:898-907. 2010
    ..The purpose of this study was to provide micrographic evidences for the damaged membrane structure and intracellular structure change of Escherichia coli strain 8099, induced by polyhexamethylene guanidine hydrochloride (PHMG)...
  89. ncbi Interactions among membrane and soluble components of the flagellar export apparatus of Salmonella
    Keng Zhu
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Biochemistry 41:9516-24. 2002
    ..FliH bound more weakly to FliI carrying the N-terminal double mutation R7C/L12P than it did to wild-type FliI, confirming the importance of the N terminus of FliI for its interaction with FliH...
  90. pmc Phospholamban modulates the functional coupling between nucleotide domains in Ca-ATPase oligomeric complexes in cardiac sarcoplasmic reticulum
    Linda T L Chen
    Biological Sciences Division, Pacific Northwest National Laboratory, P O Box 999, Richland, Washington 99352, USA
    Biochemistry 48:2411-21. 2009
    ..We suggest that the phosphorylation of PLB acts to release constraints involving interdomain subunit interactions that enhance catalytically important N-domain motions...
  91. ncbi The effect of self-association on the interaction of the Escherichia coli regulatory protein TyrR with DNA
    M F Bailey
    Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia
    J Mol Biol 263:671-84. 1996
    ....
  92. ncbi Channel-specific coatings on microfabricated chips
    L Xiong
    Department of Chemistry, Purdue University, Lafayette, IN 47907, USA
    J Chromatogr A 924:165-76. 2001
    ..Enzyme activity of immobilized beta-galactosidase (beta-Gal) was monitored by following the hydrolysis of fluorescein mono-beta-D-galactopyranoside to fluorescein with laser-induced fluorescence...
  93. pmc Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library
    B Malik
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Ohio 45267 0575
    Protein Sci 2:2103-11. 1993
    ..The M8-P1-A3 epitope was found to consist of the five amino acid 494-PRHLL-498 sequence stretch of alpha, with residues PRxLx being critical for antibody recognition...
  94. pmc Neutralization of the charge on Asp 369 of Na+,K+-ATPase triggers E1 <--> E2 conformational changes
    Talya Belogus
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 284:31038-51. 2009
    ..Changes in charge interactions of Asp(369) may play an important role in triggering E(1)P(3Na) <--> E(2)P and E(2)(2K) --> E(1)Na transitions in native Na(+),K(+)-ATPase...
  95. ncbi Characterization of and mechanism for copper-induced thioureation of serum albumin
    Yu Wei Wu
    Graduate Institute of Pharmacy and Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan
    Bioconjug Chem 19:1822-30. 2008
    ..These phenomenons may have important implications for the pharmacokinetics of thiourea-based drugs in plasma...
  96. pmc 23S rRNA assisted folding of cytoplasmic malate dehydrogenase is distinctly different from its self-folding
    Suparna Chandra Sanyal
    Department of Biophysics, Molecular Biology and Genetics, University College of Science, University of Calcutta, 92 A P C Road, Kolkata 700 009, India
    Nucleic Acids Res 30:2390-7. 2002
    ....
  97. ncbi Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of trolox
    Miriam Strosova
    Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia
    Gen Physiol Biophys 28:195-209. 2009
    ..Trolox (250 micromol/l) recovered the Ca-ATPase activity decrease induced by HOCl, probably by changing conformational properties of the Ca-ATPase protein. Trolox inhibited FITC binding to SERCA...
  98. ncbi Interaction of aluminum ions with phosvitin
    T P Geladopoulos
    Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
    J Inorg Biochem 54:247-56. 1994
    ..These findings suggest that phosvitin can be used as a model for studying interactions of aluminum with multiphosphorylated proteins and other polyanionic biopolymers...
  99. ncbi Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopy
    John T Elliott
    Biomolecular Materials Group, Biotechnology Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Cytometry A 52:90-100. 2003
    ..Generating adequate contrast at the edge of thin well-spread cells can be challenging...
  100. ncbi Von Willebrand factor accelerates platelet adhesion and thrombus formation on a collagen surface in platelet-reduced blood under flow conditions
    Kazuhiko Tomokiyo
    Blood Products Research Department, The Chemo Sero Therapeutic Research Institute, Kaketsuken, 1 6 1 Okubo, Kumamoto, 860 8568, Japan
    Blood 105:1078-84. 2005
    ..This also suggests a possibility that supplementation of VWF to individuals with thrombocytopenia might be effective for increasing their hemostatic potential...
  101. ncbi FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli
    Marc Bramkamp
    Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, D 49069 Osnabruck, Germany
    Biochemistry 43:4559-67. 2004
    ..The notion that FITC inhibits pNPPase and ATPase activity supports the idea that the catalytic domain of KdpB is much more compact than other P-type ATPases, like Na(+),K(+)-ATPase, H(+),K(+)-ATPase, and Ca(2+)-ATPase...

Research Grants64

  1. Micellar VIP Nanoparticles for Rheumatoid Arthritis
    Israel Rubinstein; Fiscal Year: 2007
    ..abstract_text> ..
  2. PGE2 Regulation of Epithelial/Fibroblast Interactions
    Bethany Moore; Fiscal Year: 2006
    ..abstract_text> ..
  3. CXC CHEMOKINE REGULATION OF PROSTATE CANCER ANGIOGENESIS
    Bethany Moore; Fiscal Year: 2002
    ..These studies should lead to new insights into the biology of prostate cancer growth and metastasis, and pave the way to adapt adjuvant therapies for the treatment of this widespread disease. ..
  4. Cerebral Microcirculation During Alcohol Consumption
    WILLIAM MAYHAN; Fiscal Year: 2008
    ..These studies will provide valuable new insights and will lead to new therapeutic approaches for the treatment of cerebrovascular abnormalities observed in chronic alcoholics. [unreadable] [unreadable]..
  5. PERITONEAL DELIVERY OF ANTINEOPLASTIC MACROMOLECULES
    MICHAEL FLESSNER; Fiscal Year: 2004
    ....
  6. Effects of Hyaluronan on Interstitial Convection
    MICHAEL FLESSNER; Fiscal Year: 2007
    ..The results of this research project will provide a basis for future work designed to preserve or enhance the transport characteristics of peritoneal tissue during chronic dialysis. ..
  7. NICOTINE INDUCED CEREBROVASCULAR DYSFUNCTION
    WILLIAM MAYHAN; Fiscal Year: 2006
    ..In addition, results of these studies may provide insights regarding possible therapeutic approaches for the treatment of nicotine-induced vascular dysfunction. ..
  8. Cerebrovascular Disease in Type 1 Diabetes
    WILLIAM MAYHAN; Fiscal Year: 2007
    ..We suggest that our results will provide novel insights regarding therapeutic approaches for the treatment/prevention of cerebrovascular dysfunction in diabetes. ..
  9. DEFINING INNATE IMMUNE RECEPTOR LIGAND BINDING SITES
    Peter Tobias; Fiscal Year: 2007
    ..By understanding how PRRs recognize PAMPs we hope to be able to propose methods for therapeutic manipulation of PAMP - PRR interactions. ..
  10. Metal-Enhanced Fluorescence Sensing
    Joseph Lakowicz; Fiscal Year: 2006
    ..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
  11. CFS Course on Fluorescence Spectroscopy
    Joseph Lakowicz; Fiscal Year: 2006
    ..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
  12. Chronic Alcohol Consumption Induces Tumor Angiogenesis
    Jian Wei Gu; Fiscal Year: 2006
    ..The new information will promote cancer prevention and treatment. ..
  13. CENTER FOR FLUORESCENCE SPECTROSCOPY
    Joseph Lakowicz; Fiscal Year: 2006
    ..Abstract Not Provided. ..
  14. Biacore T100
    Jeffrey Toretsky; Fiscal Year: 2006
    ..These enhanced capabilities of the T100 will allow projects investigating many types of biologic processes to advance. ..
  15. Whole Autologous SIV Vaccine Using Dendritic Cells
    SIMON BARRATT BOYES; Fiscal Year: 2006
    ....
  16. SkyScan 1172 High-resolution desk-top micro-CT system
    James Williams; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  17. IAPs As Novel Targets for Cancer Therapy
    Lily Yang; Fiscal Year: 2007
    ..Results from this research should allow us to determine if IAPs are novel molecular targets for the development of cancer-specific therapeutic approaches. ..
  18. Initiation of Cardiac Hypertrophy
    Subha Sen; Fiscal Year: 2008
    ..abstract_text> ..
  19. Microvascular Permeability and Sex
    VIRGINIA HUXLEY; Fiscal Year: 2008
    ....
  20. Mechanisms of Ataxin-1 Mediated Purkinje Cell Death
    PARMINDER VIG; Fiscal Year: 2007
    ..Further, changes observed in SCA-1 patients and in transgenic mice will be compared with that in Machado-Joseph disease/SCA-3, other cerebellar ataxias and normal controls. ..
  21. HIGH THROUGHPUT SCREENING FOR TOLL-LIKE RECEPTORS INHIBITORS
    Peter S Tobias; Fiscal Year: 2010
    ..The goal of this application is to develop techniques for finding new inhibitors of inflammation. ..
  22. Imaging Cell Signaling Events During Luteinizing Hormone Pulses
    DEBORAH ROESS; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  23. High Throughput Screening for Toll-Like Receptors
    Peter Tobias; Fiscal Year: 2007
    ..This project will utilize an assay that we have recently developed to search for such compounds. [unreadable] [unreadable] [unreadable]..
  24. Mesenchymal Stem Cell Homing to the Lung in Emphysema
    David Welsh; Fiscal Year: 2006
    ..These studies will expand our understanding of the biology of MSCs & methods to improve recruitment to the lung, hopefully leading to novel therapies for emphysema. ..
  25. Mechanisms and Effects of Fibronectin Matrix Remodeling
    Jane Sottile; Fiscal Year: 2007
    ....
  26. Pulmonary Mitochondrial Injury Caused by Tetrahydrocannabinol
    Theodore Sarafian; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  27. Lineage decision in blood stem and progenitor cells
    Koichi Akashi; Fiscal Year: 2007
    ..Thus, we hope these studies will help understand mechanistic effects of these transcription factors on lineage decision in normal and malignant hematopoietic development. ..
  28. New Mexico Molecular Libraries Screening Center(RMI)
    Larry Sklar; Fiscal Year: 2008
    ..Core 3, led by Co-PIs Tudor Oprea and Jeffrey Arterburn will integrate cheminformatics and synthetic chemistry teams to increase the overall efficiency of the discovery process. [unreadable] [unreadable]..
  29. Akt Inhibitors to Treat Ewing's Sarcoma
    Jeffrey Toretsky; Fiscal Year: 2005
    ..Our findings from this Phase I Sun will hopefully lead to a key compound that can be further developed in a Phase II STTR ultimately leading to clinical trials in patients with ESFT. ..
  30. Annual Meeting of the NCRR P41 Principal Investigators
    Joseph Lakowicz; Fiscal Year: 2005
    ..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
  31. Mechanisms of Pro-Thrombosis in Diabetes Mellitus
    David Schneider; Fiscal Year: 2003
    ..Further, these results will define the importance of prothrombotic potential in diabetic subjects while potentially identifying new therapeutic targets in patients with diabetes and other insulin resistant states. ..
  32. CONTROL OF CELL DIVISION IN XENOPUS OOCYTES
    JAMES MALLER; Fiscal Year: 2003
    ..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
  33. MECHANISMS REGULATING FIBRONECTIN DEPOSITION
    Jane Sottile; Fiscal Year: 2003
    ....
  34. REVERSAL OF MYOCARDIAL HYPERTROPHY IN HYPERTENSION
    Subha Sen; Fiscal Year: 2002
    ..On identifying the derangement, we will determine if appropriate treatment corrects the changes and if such directed alteration in myocardial collagen formation improves the compromised function of the hypertrophied heart. ..
  35. LH Receptor C-terminus in Receptor Desensitization
    DEBORAH ROESS; Fiscal Year: 2003
    ..Although she will develop and utilize several state-of-the- art biophysical approaches to study G-protein receptor function, this project is, nonetheless, of somewhat limited scope and likely to be completed within two years. ..
  36. Aging & Marrow Stem Cell Homing to the Lung in Emphysema
    David Welsh; Fiscal Year: 2003
    ..These studies will expand our understanding of the biology of MSCs & their potential for lung repair in the geriatric population, hopefully leading to novel therapies for emphysema. ..
  37. Nanoparticle delivery of antiangiogenic genes
    Gerard Lutty; Fiscal Year: 2003
    ..The goal of this proposal is to deliver genes for endogenous antiangiogenic agents to sites of ocular neovascularization. ..
  38. COMPLEXITY OF IN VIVO AXON GUIDANCE
    MICHAEL DAILEY; Fiscal Year: 2002
    ..The information obtained from studies of this insect are likely to be very relevant to understanding these processes in mammals. ..
  39. DYNAMICS OF CNS SYNAPSE DEVELOPMENT
    MICHAEL DAILEY; Fiscal Year: 2002
    ..Hence, these studies could provide important insight on fundamental mechanisms of synaptic development and plasticity operating over a wide range of normal and abnormal conditions in man. ..
  40. CONFOCAL MICROSCOPE
    MICHAEL DAILEY; Fiscal Year: 2003
    ..abstract_text> ..
  41. MICROCIRCULATORY PROTECTION IN LATE PRECONDITIONING
    Wei Wang; Fiscal Year: 2001
    ..The third series of experiments will determine if microvascular protection induced by adenosine, nitric oxide, protein kinase C activation, or by IP, is dependent on the activation of iNOS. ..
  42. FLOW CYTOMETRY, SAMPLE HANDLING AND CELL ADHESION
    Larry Sklar; Fiscal Year: 2003
    ..The technological capabilities that result will extend flow cytometry to ligand binding, high throughput cell screening, and automation of complex sample handling procedures. ..
  43. MOLECULAR PATHOLOGY OF LPS HOST PROTEIN COMPLEXES
    Peter Tobias; Fiscal Year: 2001
    ..Specific Aim 4: To establish the structural features of LBP important for formation of complexes with LPS and CD14. This will be done using LBP mutants, peptides derived from LPS, and anti-peptide antibodies. ..
  44. Pulmonary delivery of nicotine using the AERx System
    Stephen Farr; Fiscal Year: 2005
    ..The long-term goal of this proposed project is to develop an inhalation product (the AERx-nicotine) as a therapy for smoking cessation. ..
  45. FUNCTIONAL PROTEIN ANALYSIS--A SHARED RESOURCE FOR UNM
    Larry Sklar; Fiscal Year: 2005
    ..These state-of-the-art capabilities are expected to enhance the research scope and impact of all UNM CRTC investigators. ..
  46. MEMBRANE EVENTS FOLLOWING HORMONE BINDING TO LH RECEPTOR
    DEBORAH ROESS; Fiscal Year: 2005
    ..Colin Clay, a member of the Animal Reproduction and Biotechnology Laboratory, and of Dr. Michael Tamkun, a neurobiologist at Colorado State University. ..
  47. 2004 Biophysical Discussion: Membrane Microdomains
    Kenneth Jacobson; Fiscal Year: 2004
    ..The format of the Biophysical Discussions is unique in its stress on discussion and prior preparation by the participants. ..
  48. FLOW CYTOMETRY, SAMPLE HANDLING AND CELL ADHESION
    Larry Sklar; Fiscal Year: 2002
    ..The technological capabilities that result will extend flow cytometry to ligand binding, high throughput cell screening, and automation of complex sample handling procedures. ..
  49. VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBC
    Stephen Wagner; Fiscal Year: 2004
    ..abstract_text> ..
  50. Biohazard Detection Using Metal-Enhanced Fluorescence
    Joseph Lakowicz; Fiscal Year: 2003
    ..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
  51. Developmental Checkpoints of Murine Mast Cells
    Koichi Akashi; Fiscal Year: 2009
    ..Results obtained from this project will be critical to study mast cell biology, which eventually will help develop new therapeutic strategies for a variety of allergic and autoimmune disorders. ..
  52. Fibronectin in Cell Growth and Vascular Remodeling
    Jane Sottile; Fiscal Year: 2008
    ..These studies will provide important insights into the complex interplay between smooth muscle cells and extracellular matrix, which plays a critical role in the development and progression of vascular disease. [unreadable] [unreadable]..
  53. Signaling Pathways that Determine Ewings Sarcoma Outcome
    Jeffrey Toretsky; Fiscal Year: 2008
    ..Clearly, if PTPL1 is validated as a supportive oncoprotein and the pathways modulated by PTPL1 are identified, patients with many other tumors that rely on IGF-IR signaling including breast carcinoma could benefit from our findings. ..
  54. Neuroprotective Role of Fetuin in Cerebral Ischemia
    Haichao Wang; Fiscal Year: 2008
    ..Answers to these questions will improve our understanding of the pathophysiology of ischemic neural injury, and identify novel therapeutic agents for the treatment of stroke and related disorders. ..
  55. AMPHIPATHIC ORAL CHELATORS AND RADIONUCLIDE CONTAMINATION
    Scott Miller; Fiscal Year: 2008
    ..Because the pharmacology and toxicology of PACA chelators, such as DTPA and EDTA, are quite well understood, the development pathway of the related TT- based chelators may proceed rapidly. ..
  56. MECHANISMS OF SKELETAL RECONSTITUTION AFTER LACTATION
    Scott Miller; Fiscal Year: 2004
    ..abstract_text> ..
  57. BIOLOGY OF TRABECULAR MESHWORK IN HEALTH AND DISEASE
    BEATRICE YUE; Fiscal Year: 2009
    ..The overall goal of our proposal is to illustrate the mechanisms to provide a deeper understanding regarding the pathogenesis of glaucomas. ..
  58. BIOCHEMICAL BASIS OF KERATOCONUS
    BEATRICE YUE; Fiscal Year: 2007
    ..We hope to gain a greater understanding toward the etiologic basis of KC and provide insights to ultimately lead to prevention and treatment of this corneal disease. ..
  59. In vivo Gene Correction of Keratin 14 Gene in EBS Mouse
    Olga Igoucheva; Fiscal Year: 2007
    ..If successful, this proof-of-principle experiment will lead to a potential clinical application of oligonucleotides to severely affected areas of EB patients for treatment. [unreadable] [unreadable]..
  60. Apoptotic and Necrotic Death of OHCs in NIHL
    Bo Hua Hu; Fiscal Year: 2006
    ..The knowledge of the biological mechanisms responsible for modulation of the cell death pathways will provide the basis for eventually developing a rational protective strategy. ..