Genomes and Genes
fluorescein 5 isothiocyanate
Summary: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
Publications231 found, 100 shown here
- Activation of lysosomal function during dendritic cell maturationE Sergio Trombetta
Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520 8002, USA
Science 299:1400-3. 2003..Lysosomal function in DCs thus appears to be specialized for the developmentally regulated processing of internalized antigens...
- Transfer of antigen between dendritic cells in the stimulation of primary T cell proliferationS C Knight
Imperial College School of Medicine, Northwick Park Institute for Medical Research, Harrow, GB
Eur J Immunol 28:1636-44. 1998..We propose that this unique interaction between antigen-presenting cells may be a paradigm for self/non-self discrimination...
- ATP depletion inhibits the endocytosis of ClC-2Sonja U Dhani
Programme in Molecular Structure and Function, Research Institute in the Hospital for Sick Children, Toronto, Canada
J Cell Physiol 214:273-80. 2008..In summary, we have determined that the internalization of ClC-2 by endocytosis is inhibited by metabolic stress, highlighting the importance for understanding the molecular mechanisms mediating the endosomal trafficking of this channel...
- Dendritic cell migration controlled by alpha 1b-adrenergic receptorsG J Maestroni
Center for Experimental Pathology, Istituto Cantonale di Patologia, Locarno, Switzerland
J Immunol 165:6743-7. 2000..This finding might improve our understanding of the biological basis of skin diseases and allergic reactions, and opens new pharmacological possibilities to modulate the immune response...
- Cytopathic and non-cytopathic bovine viral diarrhoea virus biotypes affect fluid phase uptake and mannose receptor-mediated endocytosis in bovine monocytesBobbie L Boyd
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, PO Box 6100, 39762 6100, USA
Vet Immunol Immunopathol 102:53-65. 2004..By influencing the early antigen uptake function of APC, BVDV might disrupt the function of monocytes as professional APC and contribute to the specific immunotolerance to BVDV...
- Cubilin- and megalin-mediated uptake of albumin in cultured proximal tubule cells of opossum kidneyX Y Zhai
Department of Cell Biology, Institute of Anatomy and Department of Medical Biochemistry, University of Aarhus, Aarhus, Denmark
Kidney Int 58:1523-33. 2000..Two giant glycoproteins, cubilin and megalin, constitute important endocytic receptors localized to the kidney proximal tubule...
- The dendritic cell populations of mouse lymph nodesS Henri
Walter and Eliza Hall Institute of Medical Research, P O Royal Melbourne Hospital, Victoria 3050, Australia
J Immunol 167:741-8. 2001..The putative LC-derived DC among skin emigrants and in LN also showed strong intracellular staining of langerin...
- Development of a method using image analysis for the measurement of cellulose-binding domains adsorbed onto cellulose fibersRicardo Pinto
Institute for Biotechnology and Bioengineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
Biotechnol Prog 23:1492-7. 2007..By observing different spots in the surface of the fibers, site-specific information is obtained. It was verified that the physically heterogeneous fibers exhibit different amounts of adsorbed CBDs...
- Preparation and in vitro evaluation of thiolated chitosan microparticlesK Maculotti
Department of Pharmaceutical Chemistry, University of Pavia, Italy
J Microencapsul 22:459-70. 2005..5-fold higher concentration with respect to unmodified chitosan microparticles. These data suggest that chitosan-TBA conjugate microspheres have the potential to be used as a mucoadhesive drug delivery system...
- Different temperature sensitivity of endosomes involved in transport to lysosomes and transcytosis in rat hepatocytes: analysis by free-flow electrophoresisIsabella Ellinger
Department of Pathophysiology, University of Vienna, Vienna, Austria
Electrophoresis 23:2117-29. 2002....
- Biliary secretion of fluid phase markers is modified under post-cholestatic conditionsIsabella Ellinger
Department of Pathophysiology, Medical University of Vienna, Vienna, Austria
Wien Med Wochenschr 158:579-82. 2008..This fast route is taken only by HRP but not by FITC-dextran, the latter being exclusively transported by a transcellular route under all conditions investigated...
- Enhanced epidermal Langerhans cell migration in IL-10 knockout miceB Wang
Division of Dermatology, Sunnybrook Health Science Center, University of Toronto, Ontario, Canada
J Immunol 162:277-83. 1999..These data suggest that IL-10 plays an inhibitory role in LC migration and that this effect may occur via the down-regulation of TNF-alpha and IL-1 production...
- Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructureLars Hildén
WURC, Department of Wood Science, Swedish University of Agricultural Sciences, Box 7008, 750 07 Uppsala, Sweden
Biotechnol Lett 25:553-8. 2003..The labelled CBM was successfully used as a probe for detecting cellulose in lignocellulose material such as never dried spruce and birch wood as well as pulp fibres...
- Endosomal alkalinization reduces Jmax and Km of albumin receptor-mediated endocytosis in OK cellsM Gekle
Department of Physiology, , Germany
Am J Physiol 268:F899-906. 1995..Endosomal alkalinization disturbs intracellular ligand handling and receptor trafficking, leading to a reduction of endocytotic capacity and affinity...
- Extravascular transport of fluorescently labeled albumins in the rat mesenteryNorman R Harris
Department of Bioengineering, Pennsylvania State University, University Park, PA 16802 6804, USA
Microcirculation 9:177-87. 2002....
- A novel tool to characterize paracellular transport: the APTS-dextran ladderWinfried Neuhaus
Department of Medicinal Chemistry, University of Vienna, Pharmacy Center, Althanstrasse 14, A 1090, Vienna, Austria
Pharm Res 23:1491-501. 2006..The aim of this work was to develop an easy, manageable, and precise analytic tool to describe the tightness of cell layers by a molecular weight ladder...
- The uptake of Texas Red-BSA in the excretory system of schistosomes and its colocalisation with ER60 promoter-induced GFP in transiently transformed adult malesVolker Wippersteg
Institute for Genetics, Genetic Parasitology and Center for Biological and Medical Research, Heinrich Heine University, Universitatsstrasse 1, D 40225, Dusseldorf, Germany
Int J Parasitol 33:1139-43. 2003..The results suggest a role for ER60 during the host-parasite interaction. Furthermore, the colocalisation approach introduced here opens further perspectives to characterise gene-expression profiles in this parasite...
- Comparison of the endocytic properties of linear and branched PEIs, and cationic PAMAM dendrimers in B16f10 melanoma cellsF Philipp Seib
Centre for Polymer Therapeutics, Welsh School of Pharmacy, King Edward VII Avenue, Cardiff University, Cardiff CF10 3XF, UK
J Control Release 117:291-300. 2007..A perception of the rate and mechanisms of cellular uptake of these vectors will be important in the context of their proposed use as drug delivery systems...
- Chloride-dependent secretion of alveolar wall liquid determined by optical-sectioning microscopyJens Lindert
Lung Biology Laboratory, Department of Physiology and Cellular Biophysics, Columbia University, College of Physicians and Surgeons, SLRHC, New York, New York, USA
Am J Respir Cell Mol Biol 36:688-96. 2007..We conclude that CFTR-dependent liquid secretion is present in alveoli of the adult mouse. Defective alveolar secretion might impair alveolar immune defense and promote alveolar disease...
- Prevention of chronic graft-versus-host disease by stimulation with glucocorticoid-induced TNF receptorJuyang Kim
The Immunomodulation Research Center and Department of Biological Science, University of Ulsan, Ulsan 680 749, Korea
Exp Mol Med 38:94-9. 2006..Our results indicate that anti-GITR monoclonal antibody may be used as a potential immunotherapeutic agent for preventing cGVHD...
- Rapid determination of renal filtration function using an optical ratiometric imaging approachWeiming Yu
Indiana University School of Medicine, Department of Medicine, Nephrology Division, Indianapolis, IN 46202, USA
Am J Physiol Renal Physiol 292:F1873-80. 2007..This approach also offers a high sensitivity for determining plasma clearance rate of a fluorescent compound. This feature is important for rapidly quantifying small differences in plasma clearance when kidney function is changing...
- A rabbit model for monitoring in vivo viability of human platelet concentrates using flow cytometryValery Leytin
Department of Transfusion Medicine, St Michael s Hospital, Toronto, Ontario, Canada
Transfusion 42:711-8. 2002..Viability in vivo of novel platelet components cannot be readily determined in human transfusions. Elaboration of valid animal models may be useful for this purpose...
- Layer-by-layer coating of degradable microgels for pulsed drug deliveryB G De Geest
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
J Control Release 116:159-69. 2006..Further, we show that the application of an LbL coating on the surface of the microgels dramatically lowers the burst release from the microcapsules and results in massive release at the time the microcapsules rupture...
- Localization of the permeability barrier to solutes in isolated arteries by confocal microscopyPaul M A van Haaren
Department of Medical Physics, Cardiovascular Research Institute Amsterdam, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
Am J Physiol Heart Circ Physiol 285:H2848-56. 2003..This study demonstrates the presence of a thick ESL that contributes to the permeability barrier to solutes. The layer is sensitive to phototoxic stress, and its damage could form an early event in atherosclerosis...
- Nanoparticles based on N-trimethylchitosan: evaluation of absorption properties using in vitro (Caco-2 cells) and ex vivo (excised rat jejunum) modelsGiuseppina Sandri
Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy
Eur J Pharm Biopharm 65:68-77. 2007..The improvement of mucoadhesion and of nanoparticle internalisation with respect to chitosan nanosystems makes the TMCs nanosystems suitable carriers for the intestinal absorption of peptides...
- Effects of mannosylated glycopolymers on specific interaction to bone marrow hematopoietic and progenitor cells derived from murine speciesKeun Hong Park
College of Medicine, CHA Stem Cell Institute 606 16, Pochon CHA University, Yeoksam 1 dong, Kangnam Gu, Seoul 135 081, Republic of Korea
J Biomed Mater Res A 82:281-7. 2007....
- Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cellsK J de Vries
Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Biol Reprod 68:2122-34. 2003..The proportion of live cells with PL scrambling corresponded with that showing capacitation-specific chlortetracyclin staining...
- Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperatureGünther Baravalle
Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Wahringer Gurtel 18 20, 1090, Vienna, Austria
Cell Tissue Res 320:99-113. 2005..Consequently, these treatments can be applied to investigate whether internalized macromolecules such as viruses follow a recycling or degradative pathway...
- Isoprostane 8-epi-prostaglandin F2 alpha decreases lymph capillary pressure in patients with primary lymphedemaBeatrice R Amann-Vesti
Department of Medicine, Angiology Division, University Hospital, Zurich, Switzerland
J Vasc Res 40:77-82. 2003..0 +/- 7.7 mm Hg). In healthy volunteers, there was no significant decrease of LCP with PGF compared to placebo. PGF normalizes microlymphatic hypertension in patients with lymphedema by improving lymph transport into deeper channels...
- The use of quantum dots for analysis of chick CAM vasculatureJason D Smith
Institute for Complex Engineered Systems, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA
Microvasc Res 73:75-83. 2007..We also demonstrated that QDs are fixable with low fluorescence loss and thus can be used in conjunction with histological processing for further sample analysis...
- A smart membrane based on an antigen-responsive hydrogelRongsheng Zhang
Department of Chemical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, UK
Biotechnol Bioeng 97:976-84. 2007..Sequential addition and removal of sodium fluorescein showed that these permeability changes are reversible...
- Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routesSerge Camelo
School of Anatomy and Human Biology, The University of Western Australia, Crawley Perth
Invest Ophthalmol Vis Sci 47:1039-46. 2006..To determine the afferent pathways linking the anterior chamber (AC) of the eye to the secondary lymphoid organs...
- DEC-205lo Langerinlo neonatal Langerhans' cells preferentially utilize a wortmannin-sensitive, fluid-phase pathway to internalize exogenous antigenBernadette M Bellette
Discipline of Pathology, University of Tasmania, Hobart, Australia
Immunology 110:466-73. 2003..As neonatal LC are capable of sampling their environment without inducing immunity, this may serve to avoid inappropriate immune responses during the neonatal period...
- CCL5-CCR5 interaction provides antiapoptotic signals for macrophage survival during viral infectionJeffrey W Tyner
Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
Nat Med 11:1180-7. 2005..The antiapoptotic action of chemokine signaling may therefore allow scavengers to finally stop the host cell-to-cell infectious process...
- Uptake of host cell transforming growth factor-beta by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completionMariana C Waghabi
Laboratorios de Biologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
Am J Pathol 167:993-1003. 2005..These observations represent the first example of a novel mechanism by which a primitive unicellular protozoan can use host cell TGF-beta to control its own intracellular life cycle...
- Transforming growth factor-beta-dependent growth inhibition in primary vascular smooth muscle cells is p38-dependentUlrike Seay
University of Giessen Lung Center, Department of Medicine I, Aulweg 123, Room 6 11, D 35392 Giessen, Germany
J Pharmacol Exp Ther 315:1005-12. 2005..The anti-proliferative effect of TGF-beta is due to G0/G1 arrest and mediated primarily by the p38 pathway, suggesting that p38 kinase is central to TGF-beta-mediated growth inhibition in primary mouse VSMCs...
- The impact of topical antiseptics on skin microcirculationS Langer
Department of Plastic and Hand Surgery, Burn Center, BG University Hospital, Ruhr Universitat Bochum, Buerkle de la Camp Platz 1, D 44789 Bochum, Germany
Eur J Med Res 9:449-54. 2004..However, topical antiseptics are assumed to have an influence on skin microcirculation, per se. Thus, the aim of the study was to analyse the influence of topically applied antiseptics on the microcirculation of intact skin in vivo...
- Submucosal gland dysfunction as a primary defect in cystic fibrosisDanieli Salinas
Department of Medicine, University of California, San Francisco, California 94143 0521, USA
FASEB J 19:431-3. 2005..Our results suggest that defective airway submucosal gland function is an early, primary defect in CF. Therapies directed at normalizing gland fluid secretion early in CF may thus reduce lung disease...
- Effective permeability of hydrophilic substances through walls of lymph vessels: roles of endothelial barrierNobuyuki Ono
Department of Physiology, Shinshu University School of Medicine, 3 1 1 Asahi, Matsumoto, 390 8621 Japan
Am J Physiol Heart Circ Physiol 289:H1676-82. 2005....
- Synthesis, antitumor evaluation, and apoptosis-inducing activity of hydroxylated (E)-stilbenesCedric J Lion
Cancer Research U K Experimental Cancer Chemotherapy Research Group, Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, UK
J Med Chem 48:1292-5. 2005..g., 5dy and 5jy, an observation further reinforced by AnnexinV-FITC analysis and fluorescence microscopy...
- Farnesylated RhoB inhibits radiation-induced mitotic cell death and controls radiation-induced centrosome overduplicationJ Milia
INSERM U563, CPTP, Département d Innovation Thérapeutique et d Oncologie Moléculaire, Institut Claudius Regaud, 20 24 rue du Pont St Pierre, 31052 Toulouse Cedex, France
Cell Death Differ 12:492-501. 2005..Overall, our results for the first time clearly implicate farnesylated RhoB as a crucial protein in mediating cellular resistance to radiation-induced nonapoptotic cell death...
- Temporary loss of plasma membrane integrity in orthodontic tooth movementM F Orellana-Lezcano
Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada
Orthod Craniofac Res 8:106-13. 2005..These studies provide evidence of a novel cellular mechanism for uptake and release of molecules and suggest a potential role for plasma membrane disruption in the mechanotransduction of orthodontic tooth movement...
- Antimalarial quinolines and artemisinin inhibit endocytosis in Plasmodium falciparumHeinrich C Hoppe
Division of Pharmacology, University of Cape Town Medical School, Groote Schuur Hospital Old Building, Observatory, Cape Town 7925, South Africa
Antimicrob Agents Chemother 48:2370-8. 2004..These results are discussed in the light of present theories regarding the mechanisms of action of the antimalarials and highlight the potential use of drugs in manipulating and studying the endocytic pathway of malaria parasites...
- Critical effect of freezing/freeze-drying on sustained release of FITC-dextran encapsulated within PLGA microspheresTae Hyoung Kim
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, South Korea
Int J Pharm 271:207-14. 2004..Vacuum-drying was a good alternative choice in reducing the initial burst, compared to freeze-drying...
- Ciliary reorientation is evoked by a rise in calcium level over the entire ciliumYoshiaki Iwadate
Department of Life Science, Faculty of Integrated Arts and Sciences, The University of Tokushima, Tokushima, Japan
Cell Motil Cytoskeleton 57:197-206. 2004..These results strongly suggest that not only Ca2+ influx sites, but also Ca2+ binding sites and vectoral bending machineries for ciliary reversal, are distributed throughout the cilium...
- Neural tissue engineering: a self-organizing collagen guidance conduitJames B Phillips
University College London, Tissue Repair and Engineering Centre, Institute of Orthopaedics and Musculoskeletal Science, Royal National Orthopaedic Hospital, Stanmore, Middlesex, UK
Tissue Eng 11:1611-7. 2005..Neural regeneration through this device was significantly greater than in controls, demonstrating that this system has potential both as a simple robust clinical implant and as a three-dimensional engineered tissue model...
- Use of a fluorescent membrane probe to identify zooxanthellae in hospite among dissociated endoderm cell culture from coralC S Chen
Symbiosis Research Group, National Museum of Marine Biology and Aquarium, 2 Houwan Road, Checheng, Pingtung, 944 Taiwan, ROC
Protoplasma 226:175-9. 2005..This method has provided a fast and reliable assay to identify symbiotic endoderm cells and will greatly accelerate the progress of endosymbiosis research...
- Molecular characterization and immunohistochemical localization of palmdelphin, a cytosolic isoform of the paralemmin protein family implicated in membrane dynamicsBin Hu
Institut fur Physiologische Chemie, Ruhr Universitat Bochum, Germany
Eur J Cell Biol 84:853-66. 2005..These observations suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures...
- Morphology and metabolism of hepatocytes microencapsulated with acrylic terpolymer-alginate using gelatin and poly(vinyl alcohol) as extracellular matricesJ S Moon
Department of Polymer Science, Kyungpook National University, Taegu 702 701, South Korea
J Biomater Sci Polym Ed 16:1245-59. 2005..The hepatocytes encapsulated in both alginate and acrylic terpolymer with gelatin and PVA rapidly aggregated in the core. The aggregated cells showed high albumin synthesis and ammonia removal, suggesting good metabolic function...
- The ApoCorrect assay: a novel, rapid method to determine the biological functionality of radiolabeled and fluorescent Annexin A5Hendrikus H Boersma
Department of Clinical Pharmacy and Toxicology, University Hospital Maastricht, P O Box 5800, NL 6202 AZ Maastricht, The Netherlands
Anal Biochem 327:126-34. 2004..The bead assay is specific, stability-indicating, repeatable, and reproducible. It allows one to determine within 25 min the biological active fraction of a 99mTc-Annexin A5 preparation. We dubbed this assay the ApoCorrect assay...
- Pesticide mixtures potentiate the toxicity in murine thymocytesSelen Olgun
Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Toxicology 196:181-95. 2004..As more information is obtained on the potential immunotoxic effects of pesticides, further insights will be gained for the risk assessment of these environmental pollutants...
- Clustered carbohydrates as a target for natural killer cells: a model systemElena I Kovalenko
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ul Miklukho Maklaya, 16 10, Moscow 117997, Russia
Histochem Cell Biol 127:313-26. 2007..Only polymeric, or clustered, but not monomeric glycoconjugates resulted in alteration of cytotoxicity in our system, suggesting that appropriate presentation is critical for carbohydrate recognition and subsequent biological effects...
- Ca(2+) signalling and gap junction coupling within and between pigment epithelium and neural retina in the developing chickRachael A Pearson
Department of Physiology, University College London, Gower Street, London, WC1E 6BT
Eur J Neurosci 19:2435-45. 2004..We conclude that gap junctions between progenitor cells, and between these cells and the RPE, may orchestrate retinal proliferation/differentiation, via the propagation of Ca(2+) or other signalling molecules...
- Nanosecond pulsed electric fields (nsPEF) induce direct electric field effects and biological effects on human colon carcinoma cellsEmily H Hall
Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital of the King s Daughters, Norfolk, Virginia 23510, USA
DNA Cell Biol 24:283-91. 2005..NsPEF applications provide a means to explore intracellular structures and functions that can reveal mechanisms in health and disease...
- Progesterone stimulation of fluid absorption by the rat uterine glandR J Naftalin
Division of Physiology, King s College London, New Hunt s House, Guys Campus, SE1 1UL, UK
Reproduction 123:633-8. 2002..This study provides the first direct evidence of fluid absorption by the uterine glands. Such absorption may provide the mechanism for closure of the uterine lumen and immobilization of the blastocyst necessary for implantation...
- Surface expression of complement receptor gC1q-R/p33 is increased on the plasma membrane of human spermatozoa after capacitationKristen S Grace
Department of Medicine, State University of New York, Stony Brook, New York 11794 8161, USA
Biol Reprod 66:823-9. 2002..In addition, the ability of live spermatozoa to bind to monoclonal anti-gC1qR antibody-coated microtiter wells was also increased after capacitation. These results suggest that gC1qR may play a role in human fertilization...
- Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivoA C W Zannettino
Myeloma Research Program, Bone and Cancer Laboratories, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Adelaide, Australia
J Cell Physiol 214:413-21. 2008..The findings presented here, describe a multipotential stem cell population within adult human adipose tissue, which appear to be intimately associated with perivascular cells surrounding the blood vessels...
- In vivo fluorescence measurement of Na(+) concentration in the pericryptal space of mouse descending colonJ R Thiagarajah
Division of Physiology, Centre for Vascular Biology and Medicine, King's College London, Guy's Campus, London SE1 1UL, United Kingdom
Am J Physiol Cell Physiol 281:C1898-903. 2001..The pericryptal space represents the first example of a hypertonic extracellular compartment in mammals that is not created by a countercurrent amplification mechanism...
- Geometric characteristics of arterial network of rat pial microcirculationD Lapi
Department of Physiology and Biochemistry, University of Pisa, Pisa, Italy
J Vasc Res 45:69-77. 2008..The aim of the study was to assess the geometric characteristics of rat pial microcirculation and describe the vessel bifurcation patterns by 'connectivity matrix'...
- A miniature head-mounted two-photon microscope. high-resolution brain imaging in freely moving animalsF Helmchen
Biological Computation Research Department, Bell Laboratories, Lucent Technologies, Murray Hill, NJ 07974, USA
Neuron 31:903-12. 2001..Capillary blood flow and dendritic calcium transients were measured with high time resolution using line scans. In awake, freely moving rats, stable imaging was possible except during sudden head movements...
- Characterization of protein factor(s) in rat bronchoalveolar lavage fluid that enhance insulin transport via transcytosis across primary rat alveolar epithelial cell monolayersRana Bahhady
Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90089 9121, USA
Eur J Pharm Biopharm 69:808-16. 2008....
- In vivo confocal laser laparoscopy allows real time subsurface microscopy in animal models of liver diseaseMartin Goetz
I Medizinische Klinik und Poliklinik, Johannes Gutenberg Universitat Mainz, Germany
J Hepatol 48:91-7. 2008..Therefore a newly developed miniaturised confocal probe was evaluated for in vivo microscopic imaging in rodent models of human liver diseases...
- Acute effects of whole-body exposure to static magnetic fields and 50-Hz electromagnetic fields on muscle microcirculation in anesthetized miceS Xu
Department of Physiological Hygiene, National Institute of Public Health, Tokyo, Japan
Bioelectrochemistry 53:127-35. 2001..These findings suggest that field intensity of 1 mT might be considered as a threshold level for enhancing muscle microcirculation under pentobarbital-induced hypnosis...
- The differential influence of non-iodinated and mono- or diiodinated benzoic acids on cellular and nuclear uptake of the nuclear localization sequence of the SV 40 T antigenStefan Heckl
Department of Neuroradiology, University of Tubingen, Germany
Int J Pharm 355:131-40. 2008..Such mono- or diiodobenzoic acid conjugates may therefore have potential in the development of new non-radioactive drugs against malignant glioma cells...
- Intravital microscopic analysis of vascular perfusion and macromolecule extravasation after photodynamic vascular targeting therapyChong He
Department of Pharmaceutical Sciences, Philadelphia College of Pharmacy, University of the Sciences in Philadelphia, 600 South 43rd Street, Philadelphia, Pennsylvania 19104, USA
Pharm Res 25:1873-80. 2008..Since tumor vasculature is the common route for the delivery of both blood and therapeutic agents, it is important to compare the effects of PDT on blood perfusion and substance transport...
- Generation of stable concentration gradients in 2D and 3D environments using a microfluidic ladder chamberWajeeh Saadi
Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science II, Irvine, CA 92697, USA
Biomed Microdevices 9:627-35. 2007..The Ladder Chamber's simple design and experimental implementation make it an attractive approach for investigating cell migration and other biological experiments in well-defined gradients in 2D surfaces as well as in 3D gels...
- Investigation of antigen delivery route in vivo and imune-boosting effects mediated by pH-sensitive liposomes encapsulated with K(b)-restricted CTL epitopeKi Young Lee
Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, 134 Shinchon dong, Seodaemun gu, Seoul, 120 749, South Korea
Biochem Biophys Res Commun 292:682-8. 2002..These results suggest that pH-sensitive liposome formula functions as a potential adjuvant of peptide antigens and is useful for the induction of antigen specific CTLsv in vivo...
- A novel imaging technique for experimental choroidal neovascularizationMercedes Campos
Biological Imaging Core, National Eye Institute, NIH, Bethesda, Maryland 20892 0703, USA
Invest Ophthalmol Vis Sci 47:5163-70. 2006..The authors developed an imaging technique for visualizing and quantifying morphologic changes associated with experimental laser-induced CNV...
- The BEACH protein LvsB is localized on lysosomes and postlysosomes and limits their fusion with early endosomesElena Kypri
Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Traffic 8:774-83. 2007..These results support the model that LvsB and Lyst proteins act as negative regulators of fusion by limiting the heterotypic fusion of early endosomes with postlysosomal compartments...
- Combination of doxorubicin and low-intensity ultrasound causes a synergistic enhancement in cell killing and an additive enhancement in apoptosis induction in human lymphoma U937 cellsToru Yoshida
Second Department of Surgery, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, 930 0194, Japan
Cancer Chemother Pharmacol 61:559-67. 2008..Here, the combined effects of low-intensity pulsed US and doxorubicin (DOX) on cell killing and apoptosis induction of U937 cells, and mechanisms involved were investigated...
- NS-398, a cyclooxygenase-2-specific inhibitor, delays skeletal muscle healing by decreasing regeneration and promoting fibrosisWei Shen
Departments of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA
Am J Pathol 167:1105-17. 2005..Our findings suggest that the use of cyclooxygenase-2-specific inhibitors to treat skeletal muscle injuries warrants caution because they may interfere with muscle healing...
- Inhibition of proliferation and apoptosis of vascular smooth muscle cells by ghrelinMin Zhan
The Affiliated Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China
Acta Biochim Biophys Sin (Shanghai) 40:769-76. 2008..Furthermore, the former effect is probably mediated by the growth hormone secretagogue receptor type 1a receptor, while the latter effect may be mediated through other receptors...
- Intravital fluorescence microscopy and phototocicity: effects on leukocytesAnthony G Harris
Institute for Surgical Research, Klinikum Grosshadern, Marchioninistr 15, D 81366 Munich, Germany
Eur J Med Res 7:117-24. 2002....
- Targeted inactivation of murine band 3 (AE1) gene produces a hypercoagulable state causing widespread thrombosis in vivoH Hassoun
Department of Biomedical Research, St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, MA, USA
Blood 92:1785-92. 1998....
- Determination of macromolecular exchange and PO2 in the microcirculation: a simple system for in vivo fluorescence and phosphorescence videomicroscopyL N Torres
, , Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, RJ, Brasil
Braz J Med Biol Res 34:129-35. 2001..05 to 0.5 mg/ml). The system was successfully used to study macromolecular extravasation and PO2 in the rat mesentery circulation under controlled conditions and during ischemia-reperfusion...
- Fluorescence resonance energy transfer between points on tropomyosin and actin in skeletal muscle thin filaments: does tropomyosin move?M Miki
Department of Applied Chemistry and Biotechnology, Fukui University, Fukui, 910 8507, Japan
J Biochem 123:1104-11. 1998..The present results do not support the notion of tropomyosin movement on skeletal muscle thin filaments as proposed in the steric blocking theory...
- A selective reaction of fructose bisphosphate aldolase with fluorescein isothiocyanate in chicken muscle extractsAndrew G Gehring
United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
J Mol Recognit 21:137-47. 2008..Unlike chicken and rabbit muscle aldolases, chicken brain and liver aldolase isoforms along with several other aldolases derived from diverse biological sources did not exhibit this highly selective modification by 5'-FITC...
- Identification of arginyl residues located at the ATP binding site of sarcoplasmic reticulum Ca2+-ATPase. Modification with 1,2-cyclohexanedioneK Kimura
Department of Biochemistry, Asahikawa Medical College, Asahikawa 078, Japan
J Biol Chem 271:28933-41. 1996..This suggests that the arginyl residues involved in these inhibitions are distinct from that involved in the inhibition of the Ca2+-ATPase activity...
- [Characteristics and in vitro imaging study of matrix metalloproteinase-2 targeting activable cell-penetrating peptide]Min Liu
Imaging Center, The 2nd Affiliated Hospital of Medical School, Xi an Jiao Tong University, Xi an 710004, China
Zhonghua Yi Xue Za Zhi 87:233-9. 2007..To study invasively imaging MMP2-positive tumor cell by paramagnetic Gadolinium or fluorescein carried by a activable cell penetrating peptides...
- Ligands presumed to label high affinity and low affinity ATP binding sites do not interact in an (alpha beta)2 diprotomer in duck nasal gland Na+,K+-ATPase, nor Do the sites coexist in native enzymeD W Martin
Division of Hematology, Department of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794 8151, USA
J Biol Chem 275:24512-7. 2000..Low affinity sites do not exist on the unmodified enzyme but can be detected only after the high affinity site is modified by FITC...
- Autonomous folding of the recombinant large cytoplasmic loop of sarcoplasmic reticulum Ca2+-ATPase probed by affinity labeling and trypsin digestionM J Moutin
Laboratoire de Biophysique Moléculaire et Cellulaire, URA 520 CNRS, CEA, DBMS CENG, Grenoble, France
Eur J Biochem 251:682-90. 1998..The hinge region does not appear to be part of the FITC-binding site but constitutes portions of the 2',3'-O-(2,4,6-trinitrophenyl)-ATP and, probably, ATP-binding site...
- Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPaseKazuhiro Abe
Biological Chemistry, Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
J Biochem 138:293-301. 2005....
- Sub-lethal photodynamic damage to ARPE-19 cells transiently inhibits their phagocytic activityMagdalena Olchawa
Department of Biophysics, Jagiellonian University, Krakow, Poland
Photochem Photobiol 86:772-80. 2010..We postulate that oxidative damage to key components of the cell phagocytic machinery may be responsible for severe impairment of its activity, which can lead to retinal degeneration...
- Characterization of interactions involving anti-metatype antibodies and immune complexesK M Weidner
Department of Microbiology, University of Illinois, Urbana 61801
Mol Immunol 29:303-12. 1992....
- Effects of a novel pathogenesis-related class 10 (PR-10) protein from Crotalaria pallida Roots with papain inhibitory activity against root-knot nematode Meloidogyne incognitaLúcia Betânia da S Andrade
Centro de Ciencias Agrarias e Biologicas, Universidade Estadual Vale do Acarau UVA, Sobral, Ceara CE, Brazil
J Agric Food Chem 58:4145-52. 2010..In a localization study, fluorescein-5-isothiocyanate (FITC)-CpPRI was observed to internalize and diffuse over the entire J2 body after 6 h of incubation. This fact could explain the natural tolerance of this plant species to nematodes...
- Killing of Candida albicans by histatin 5: cellular uptake and energy requirementC Gyurko
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Massachusetts 02118 2392, USA
Antonie Van Leeuwenhoek 79:297-309. 2001..These results indicated that histatin 5 killing activity could be directly correlated to histatin 5 internalization. Both of these processes are prevented by modulators of cellular metabolic activity...
- Cellubrevin-targeted fluorescence uncovers heterogeneity in the recycling endosomesK Teter
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
J Biol Chem 273:19625-33. 1998..Based on the combination of these morphological and functional data, we suggest that the recycling endosomes are heterogeneous in their biochemical compositions, ion transport properties, and pH values...
- Damage of Escherichia coli membrane by bactericidal agent polyhexamethylene guanidine hydrochloride: micrographic evidencesZ x Zhou
State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China
J Appl Microbiol 108:898-907. 2010..The purpose of this study was to provide micrographic evidences for the damaged membrane structure and intracellular structure change of Escherichia coli strain 8099, induced by polyhexamethylene guanidine hydrochloride (PHMG)...
- Interactions among membrane and soluble components of the flagellar export apparatus of SalmonellaKeng Zhu
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
Biochemistry 41:9516-24. 2002..FliH bound more weakly to FliI carrying the N-terminal double mutation R7C/L12P than it did to wild-type FliI, confirming the importance of the N terminus of FliI for its interaction with FliH...
- Phospholamban modulates the functional coupling between nucleotide domains in Ca-ATPase oligomeric complexes in cardiac sarcoplasmic reticulumLinda T L Chen
Biological Sciences Division, Pacific Northwest National Laboratory, P O Box 999, Richland, Washington 99352, USA
Biochemistry 48:2411-21. 2009..We suggest that the phosphorylation of PLB acts to release constraints involving interdomain subunit interactions that enhance catalytically important N-domain motions...
- The effect of self-association on the interaction of the Escherichia coli regulatory protein TyrR with DNAM F Bailey
Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia
J Mol Biol 263:671-84. 1996....
- Channel-specific coatings on microfabricated chipsL Xiong
Department of Chemistry, Purdue University, Lafayette, IN 47907, USA
J Chromatogr A 924:165-76. 2001..Enzyme activity of immobilized beta-galactosidase (beta-Gal) was monitored by following the hydrolysis of fluorescein mono-beta-D-galactopyranoside to fluorescein with laser-induced fluorescence...
- Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide libraryB Malik
Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Ohio 45267 0575
Protein Sci 2:2103-11. 1993..The M8-P1-A3 epitope was found to consist of the five amino acid 494-PRHLL-498 sequence stretch of alpha, with residues PRxLx being critical for antibody recognition...
- Neutralization of the charge on Asp 369 of Na+,K+-ATPase triggers E1 <--> E2 conformational changesTalya Belogus
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 284:31038-51. 2009..Changes in charge interactions of Asp(369) may play an important role in triggering E(1)P(3Na) <--> E(2)P and E(2)(2K) --> E(1)Na transitions in native Na(+),K(+)-ATPase...
- Characterization of and mechanism for copper-induced thioureation of serum albuminYu Wei Wu
Graduate Institute of Pharmacy and Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan
Bioconjug Chem 19:1822-30. 2008..These phenomenons may have important implications for the pharmacokinetics of thiourea-based drugs in plasma...
- 23S rRNA assisted folding of cytoplasmic malate dehydrogenase is distinctly different from its self-foldingSuparna Chandra Sanyal
Department of Biophysics, Molecular Biology and Genetics, University College of Science, University of Calcutta, 92 A P C Road, Kolkata 700 009, India
Nucleic Acids Res 30:2390-7. 2002....
- Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of troloxMiriam Strosova
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia
Gen Physiol Biophys 28:195-209. 2009..Trolox (250 micromol/l) recovered the Ca-ATPase activity decrease induced by HOCl, probably by changing conformational properties of the Ca-ATPase protein. Trolox inhibited FITC binding to SERCA...
- Interaction of aluminum ions with phosvitinT P Geladopoulos
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
J Inorg Biochem 54:247-56. 1994..These findings suggest that phosvitin can be used as a model for studying interactions of aluminum with multiphosphorylated proteins and other polyanionic biopolymers...
- Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopyJohn T Elliott
Biomolecular Materials Group, Biotechnology Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Cytometry A 52:90-100. 2003..Generating adequate contrast at the edge of thin well-spread cells can be challenging...
- Von Willebrand factor accelerates platelet adhesion and thrombus formation on a collagen surface in platelet-reduced blood under flow conditionsKazuhiko Tomokiyo
Blood Products Research Department, The Chemo Sero Therapeutic Research Institute, Kaketsuken, 1 6 1 Okubo, Kumamoto, 860 8568, Japan
Blood 105:1078-84. 2005..This also suggests a possibility that supplementation of VWF to individuals with thrombocytopenia might be effective for increasing their hemostatic potential...
- FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coliMarc Bramkamp
Abteilung Mikrobiologie, Fachbereich Biologie chemie, Universitat Osnabruck, D 49069 Osnabruck, Germany
Biochemistry 43:4559-67. 2004..The notion that FITC inhibits pNPPase and ATPase activity supports the idea that the catalytic domain of KdpB is much more compact than other P-type ATPases, like Na(+),K(+)-ATPase, H(+),K(+)-ATPase, and Ca(2+)-ATPase...
- Micellar VIP Nanoparticles for Rheumatoid ArthritisIsrael Rubinstein; Fiscal Year: 2007..abstract_text> ..
- PGE2 Regulation of Epithelial/Fibroblast InteractionsBethany Moore; Fiscal Year: 2006..abstract_text> ..
- CXC CHEMOKINE REGULATION OF PROSTATE CANCER ANGIOGENESISBethany Moore; Fiscal Year: 2002..These studies should lead to new insights into the biology of prostate cancer growth and metastasis, and pave the way to adapt adjuvant therapies for the treatment of this widespread disease. ..
- Cerebral Microcirculation During Alcohol ConsumptionWILLIAM MAYHAN; Fiscal Year: 2008..These studies will provide valuable new insights and will lead to new therapeutic approaches for the treatment of cerebrovascular abnormalities observed in chronic alcoholics. [unreadable] [unreadable]..
- PERITONEAL DELIVERY OF ANTINEOPLASTIC MACROMOLECULESMICHAEL FLESSNER; Fiscal Year: 2004....
- Effects of Hyaluronan on Interstitial ConvectionMICHAEL FLESSNER; Fiscal Year: 2007..The results of this research project will provide a basis for future work designed to preserve or enhance the transport characteristics of peritoneal tissue during chronic dialysis. ..
- NICOTINE INDUCED CEREBROVASCULAR DYSFUNCTIONWILLIAM MAYHAN; Fiscal Year: 2006..In addition, results of these studies may provide insights regarding possible therapeutic approaches for the treatment of nicotine-induced vascular dysfunction. ..
- Cerebrovascular Disease in Type 1 DiabetesWILLIAM MAYHAN; Fiscal Year: 2007..We suggest that our results will provide novel insights regarding therapeutic approaches for the treatment/prevention of cerebrovascular dysfunction in diabetes. ..
- DEFINING INNATE IMMUNE RECEPTOR LIGAND BINDING SITESPeter Tobias; Fiscal Year: 2007..By understanding how PRRs recognize PAMPs we hope to be able to propose methods for therapeutic manipulation of PAMP - PRR interactions. ..
- Metal-Enhanced Fluorescence SensingJoseph Lakowicz; Fiscal Year: 2006..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
- CFS Course on Fluorescence SpectroscopyJoseph Lakowicz; Fiscal Year: 2006..They also plan to send announcements to other P41 centers to encourage collaborations between centers. They are requesting funds adequate to provide 10 complete fee waivers in the first year, growing to 18 waivers in the fifth year. ..
- Chronic Alcohol Consumption Induces Tumor AngiogenesisJian Wei Gu; Fiscal Year: 2006..The new information will promote cancer prevention and treatment. ..
- CENTER FOR FLUORESCENCE SPECTROSCOPYJoseph Lakowicz; Fiscal Year: 2006..Abstract Not Provided. ..
- Biacore T100Jeffrey Toretsky; Fiscal Year: 2006..These enhanced capabilities of the T100 will allow projects investigating many types of biologic processes to advance. ..
- Whole Autologous SIV Vaccine Using Dendritic CellsSIMON BARRATT BOYES; Fiscal Year: 2006....
- SkyScan 1172 High-resolution desk-top micro-CT systemJames Williams; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- IAPs As Novel Targets for Cancer TherapyLily Yang; Fiscal Year: 2007..Results from this research should allow us to determine if IAPs are novel molecular targets for the development of cancer-specific therapeutic approaches. ..
- Initiation of Cardiac HypertrophySubha Sen; Fiscal Year: 2008..abstract_text> ..
- Microvascular Permeability and SexVIRGINIA HUXLEY; Fiscal Year: 2008....
- Mechanisms of Ataxin-1 Mediated Purkinje Cell DeathPARMINDER VIG; Fiscal Year: 2007..Further, changes observed in SCA-1 patients and in transgenic mice will be compared with that in Machado-Joseph disease/SCA-3, other cerebellar ataxias and normal controls. ..
- HIGH THROUGHPUT SCREENING FOR TOLL-LIKE RECEPTORS INHIBITORSPeter S Tobias; Fiscal Year: 2010..The goal of this application is to develop techniques for finding new inhibitors of inflammation. ..
- Imaging Cell Signaling Events During Luteinizing Hormone PulsesDEBORAH ROESS; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- High Throughput Screening for Toll-Like ReceptorsPeter Tobias; Fiscal Year: 2007..This project will utilize an assay that we have recently developed to search for such compounds. [unreadable] [unreadable] [unreadable]..
- Mesenchymal Stem Cell Homing to the Lung in EmphysemaDavid Welsh; Fiscal Year: 2006..These studies will expand our understanding of the biology of MSCs & methods to improve recruitment to the lung, hopefully leading to novel therapies for emphysema. ..
- Mechanisms and Effects of Fibronectin Matrix RemodelingJane Sottile; Fiscal Year: 2007....
- Pulmonary Mitochondrial Injury Caused by TetrahydrocannabinolTheodore Sarafian; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Lineage decision in blood stem and progenitor cellsKoichi Akashi; Fiscal Year: 2007..Thus, we hope these studies will help understand mechanistic effects of these transcription factors on lineage decision in normal and malignant hematopoietic development. ..
- New Mexico Molecular Libraries Screening Center(RMI)Larry Sklar; Fiscal Year: 2008..Core 3, led by Co-PIs Tudor Oprea and Jeffrey Arterburn will integrate cheminformatics and synthetic chemistry teams to increase the overall efficiency of the discovery process. [unreadable] [unreadable]..
- Akt Inhibitors to Treat Ewing's SarcomaJeffrey Toretsky; Fiscal Year: 2005..Our findings from this Phase I Sun will hopefully lead to a key compound that can be further developed in a Phase II STTR ultimately leading to clinical trials in patients with ESFT. ..
- Annual Meeting of the NCRR P41 Principal InvestigatorsJoseph Lakowicz; Fiscal Year: 2005..Based on the success of this years meeting the annual P41 meeting will become a valued event for the PIs which will serve to minimize overlap and foster collaborations between the Centers. ..
- Mechanisms of Pro-Thrombosis in Diabetes MellitusDavid Schneider; Fiscal Year: 2003..Further, these results will define the importance of prothrombotic potential in diabetic subjects while potentially identifying new therapeutic targets in patients with diabetes and other insulin resistant states. ..
- CONTROL OF CELL DIVISION IN XENOPUS OOCYTESJAMES MALLER; Fiscal Year: 2003..Further work will examine the regulation, localization, and targets of PIx1 and identify kinases that activate xPIkk1. ..
- MECHANISMS REGULATING FIBRONECTIN DEPOSITIONJane Sottile; Fiscal Year: 2003....
- REVERSAL OF MYOCARDIAL HYPERTROPHY IN HYPERTENSIONSubha Sen; Fiscal Year: 2002..On identifying the derangement, we will determine if appropriate treatment corrects the changes and if such directed alteration in myocardial collagen formation improves the compromised function of the hypertrophied heart. ..
- LH Receptor C-terminus in Receptor DesensitizationDEBORAH ROESS; Fiscal Year: 2003..Although she will develop and utilize several state-of-the- art biophysical approaches to study G-protein receptor function, this project is, nonetheless, of somewhat limited scope and likely to be completed within two years. ..
- Aging & Marrow Stem Cell Homing to the Lung in EmphysemaDavid Welsh; Fiscal Year: 2003..These studies will expand our understanding of the biology of MSCs & their potential for lung repair in the geriatric population, hopefully leading to novel therapies for emphysema. ..
- Nanoparticle delivery of antiangiogenic genesGerard Lutty; Fiscal Year: 2003..The goal of this proposal is to deliver genes for endogenous antiangiogenic agents to sites of ocular neovascularization. ..
- COMPLEXITY OF IN VIVO AXON GUIDANCEMICHAEL DAILEY; Fiscal Year: 2002..The information obtained from studies of this insect are likely to be very relevant to understanding these processes in mammals. ..
- DYNAMICS OF CNS SYNAPSE DEVELOPMENTMICHAEL DAILEY; Fiscal Year: 2002..Hence, these studies could provide important insight on fundamental mechanisms of synaptic development and plasticity operating over a wide range of normal and abnormal conditions in man. ..
- CONFOCAL MICROSCOPEMICHAEL DAILEY; Fiscal Year: 2003..abstract_text> ..
- MICROCIRCULATORY PROTECTION IN LATE PRECONDITIONINGWei Wang; Fiscal Year: 2001..The third series of experiments will determine if microvascular protection induced by adenosine, nitric oxide, protein kinase C activation, or by IP, is dependent on the activation of iNOS. ..
- FLOW CYTOMETRY, SAMPLE HANDLING AND CELL ADHESIONLarry Sklar; Fiscal Year: 2003..The technological capabilities that result will extend flow cytometry to ligand binding, high throughput cell screening, and automation of complex sample handling procedures. ..
- MOLECULAR PATHOLOGY OF LPS HOST PROTEIN COMPLEXESPeter Tobias; Fiscal Year: 2001..Specific Aim 4: To establish the structural features of LBP important for formation of complexes with LPS and CD14. This will be done using LBP mutants, peptides derived from LPS, and anti-peptide antibodies. ..
- Pulmonary delivery of nicotine using the AERx SystemStephen Farr; Fiscal Year: 2005..The long-term goal of this proposed project is to develop an inhalation product (the AERx-nicotine) as a therapy for smoking cessation. ..
- FUNCTIONAL PROTEIN ANALYSIS--A SHARED RESOURCE FOR UNMLarry Sklar; Fiscal Year: 2005..These state-of-the-art capabilities are expected to enhance the research scope and impact of all UNM CRTC investigators. ..
- MEMBRANE EVENTS FOLLOWING HORMONE BINDING TO LH RECEPTORDEBORAH ROESS; Fiscal Year: 2005..Colin Clay, a member of the Animal Reproduction and Biotechnology Laboratory, and of Dr. Michael Tamkun, a neurobiologist at Colorado State University. ..
- 2004 Biophysical Discussion: Membrane MicrodomainsKenneth Jacobson; Fiscal Year: 2004..The format of the Biophysical Discussions is unique in its stress on discussion and prior preparation by the participants. ..
- FLOW CYTOMETRY, SAMPLE HANDLING AND CELL ADHESIONLarry Sklar; Fiscal Year: 2002..The technological capabilities that result will extend flow cytometry to ligand binding, high throughput cell screening, and automation of complex sample handling procedures. ..
- VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBCStephen Wagner; Fiscal Year: 2004..abstract_text> ..
- Biohazard Detection Using Metal-Enhanced FluorescenceJoseph Lakowicz; Fiscal Year: 2003..pestis. The sensitivity of the assays will be determined by assays based on serial dilutions. The specificity will be determined against DNA from other species and strains of Yersinia. ..
- Developmental Checkpoints of Murine Mast CellsKoichi Akashi; Fiscal Year: 2009..Results obtained from this project will be critical to study mast cell biology, which eventually will help develop new therapeutic strategies for a variety of allergic and autoimmune disorders. ..
- Fibronectin in Cell Growth and Vascular RemodelingJane Sottile; Fiscal Year: 2008..These studies will provide important insights into the complex interplay between smooth muscle cells and extracellular matrix, which plays a critical role in the development and progression of vascular disease. [unreadable] [unreadable]..
- Signaling Pathways that Determine Ewings Sarcoma OutcomeJeffrey Toretsky; Fiscal Year: 2008..Clearly, if PTPL1 is validated as a supportive oncoprotein and the pathways modulated by PTPL1 are identified, patients with many other tumors that rely on IGF-IR signaling including breast carcinoma could benefit from our findings. ..
- Neuroprotective Role of Fetuin in Cerebral IschemiaHaichao Wang; Fiscal Year: 2008..Answers to these questions will improve our understanding of the pathophysiology of ischemic neural injury, and identify novel therapeutic agents for the treatment of stroke and related disorders. ..
- AMPHIPATHIC ORAL CHELATORS AND RADIONUCLIDE CONTAMINATIONScott Miller; Fiscal Year: 2008..Because the pharmacology and toxicology of PACA chelators, such as DTPA and EDTA, are quite well understood, the development pathway of the related TT- based chelators may proceed rapidly. ..
- MECHANISMS OF SKELETAL RECONSTITUTION AFTER LACTATIONScott Miller; Fiscal Year: 2004..abstract_text> ..
- BIOLOGY OF TRABECULAR MESHWORK IN HEALTH AND DISEASEBEATRICE YUE; Fiscal Year: 2009..The overall goal of our proposal is to illustrate the mechanisms to provide a deeper understanding regarding the pathogenesis of glaucomas. ..
- BIOCHEMICAL BASIS OF KERATOCONUSBEATRICE YUE; Fiscal Year: 2007..We hope to gain a greater understanding toward the etiologic basis of KC and provide insights to ultimately lead to prevention and treatment of this corneal disease. ..
- In vivo Gene Correction of Keratin 14 Gene in EBS MouseOlga Igoucheva; Fiscal Year: 2007..If successful, this proof-of-principle experiment will lead to a potential clinical application of oligonucleotides to severely affected areas of EB patients for treatment. [unreadable] [unreadable]..
- Apoptotic and Necrotic Death of OHCs in NIHLBo Hua Hu; Fiscal Year: 2006..The knowledge of the biological mechanisms responsible for modulation of the cell death pathways will provide the basis for eventually developing a rational protective strategy. ..