peroxynitrous acid

Summary

Summary: A potent oxidant synthesized by the cell during its normal metabolism. Peroxynitrite is formed from the reaction of two free radicals, NITRIC OXIDE and the superoxide anion (SUPEROXIDES).

Top Publications

  1. pmc Nitric oxide and peroxynitrite in health and disease
    Pal Pacher
    Section on Oxidative Stress Tissue Injury, Laboratory of Physiologic Studies, National Institutes of Health, National Institute of Alcohol Abuse and Alcoholism, Bethesda, Maryland, USA
    Physiol Rev 87:315-424. 2007
  2. ncbi Peroxynitrite: biochemistry, pathophysiology and development of therapeutics
    Csaba Szabo
    Department of Surgery, University of Medicine and Dentistry of New Jersey, 185 South Orange Avenue, University Heights, Newark, New Jersey 07103 2714, USA
    Nat Rev Drug Discov 6:662-80. 2007
  3. ncbi Race-specific differences in endothelial function: predisposition of African Americans to vascular diseases
    Leszek Kalinowski
    Department of Biochemistry, Ohio University, Athens, OH 45701, USA
    Circulation 109:2511-7. 2004
  4. doi Contribution of nitric oxide, superoxide anion, and peroxynitrite to activation of mitochondrial apoptotic signaling in hippocampal CA3 subfield following experimental temporal lobe status epilepticus
    Yao Chung Chuang
    Department of Neurology, Chang Gung Memorial Hospital Kaohsiung Medical Center, Chang Gung University College of Medicine, Taiwan, Republic of China
    Epilepsia 50:731-46. 2009
  5. ncbi Diabetes-induced nitrative stress in the retina, and correction by aminoguanidine
    Yunpeng Du
    Department of Medicine, Center for Diabetes Research, Case Western Reserve University, University Hospitals, and Veterans Affairs Medical Center, Cleveland, Ohio 44106 4951, USA
    J Neurochem 80:771-9. 2002
  6. doi Endogenous peroxynitrite modulates PGHS-1-dependent thromboxane A2 formation and aggregation in human platelets
    Stefan Schildknecht
    Vascular Biology Unit, Boston University School of Medicine, Boston, MA, 02118, USA
    Free Radic Biol Med 45:512-20. 2008
  7. doi Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation
    Francesc X Guix
    Laboratory of Molecular Physiology and Channelopathies, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain
    Brain 132:1335-45. 2009
  8. ncbi A computational model for free radicals transport in the microcirculation
    Mahendra Kavdia
    Biomedical Engineering Program, University of Arkansas, Fayetteville, 72701, USA
    Antioxid Redox Signal 8:1103-11. 2006
  9. doi Mechanistic studies on the reaction between cob(II)alamin and peroxynitrite: evidence for a dual role for cob(II)alamin as a scavenger of peroxynitrous acid and nitrogen dioxide
    Riya Mukherjee
    Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA
    Chemistry 17:11805-12. 2011
  10. ncbi Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure
    Ivana Serraino
    Dipartimento Clinico e Sperimentale di Medicina e Farmacologia, Torre Biologica, Policlinico Universitario, 98123 Messina, Italy
    Life Sci 73:1097-114. 2003

Detail Information

Publications223 found, 100 shown here

  1. pmc Nitric oxide and peroxynitrite in health and disease
    Pal Pacher
    Section on Oxidative Stress Tissue Injury, Laboratory of Physiologic Studies, National Institutes of Health, National Institute of Alcohol Abuse and Alcoholism, Bethesda, Maryland, USA
    Physiol Rev 87:315-424. 2007
    ..Hence, novel pharmacological strategies aimed at removing peroxynitrite might represent powerful therapeutic tools in the future. Evidence supporting these novel roles of NO and peroxynitrite is presented in detail in this review...
  2. ncbi Peroxynitrite: biochemistry, pathophysiology and development of therapeutics
    Csaba Szabo
    Department of Surgery, University of Medicine and Dentistry of New Jersey, 185 South Orange Avenue, University Heights, Newark, New Jersey 07103 2714, USA
    Nat Rev Drug Discov 6:662-80. 2007
    ..These include its catalytic reduction to nitrite and its isomerization to nitrate by metalloporphyrins, which have led to potential candidates for drug development for cardiovascular, inflammatory and neurodegenerative diseases...
  3. ncbi Race-specific differences in endothelial function: predisposition of African Americans to vascular diseases
    Leszek Kalinowski
    Department of Biochemistry, Ohio University, Athens, OH 45701, USA
    Circulation 109:2511-7. 2004
    ..We hypothesized that the differences in endothelial NO/O2-/ONOO- metabolism may highlight the potential predisposition to endothelial dysfunction and cardiovascular complications prevalent in blacks...
  4. doi Contribution of nitric oxide, superoxide anion, and peroxynitrite to activation of mitochondrial apoptotic signaling in hippocampal CA3 subfield following experimental temporal lobe status epilepticus
    Yao Chung Chuang
    Department of Neurology, Chang Gung Memorial Hospital Kaohsiung Medical Center, Chang Gung University College of Medicine, Taiwan, Republic of China
    Epilepsia 50:731-46. 2009
    ....
  5. ncbi Diabetes-induced nitrative stress in the retina, and correction by aminoguanidine
    Yunpeng Du
    Department of Medicine, Center for Diabetes Research, Case Western Reserve University, University Hospitals, and Veterans Affairs Medical Center, Cleveland, Ohio 44106 4951, USA
    J Neurochem 80:771-9. 2002
    ..Hyperglycemia increases NO production in retinal cells, and aminoguanidine can inhibit this abnormality. Inhibition of diabetic retinopathy by aminoguanidine might be mediated in part by inhibition of sequelae of NO production...
  6. doi Endogenous peroxynitrite modulates PGHS-1-dependent thromboxane A2 formation and aggregation in human platelets
    Stefan Schildknecht
    Vascular Biology Unit, Boston University School of Medicine, Boston, MA, 02118, USA
    Free Radic Biol Med 45:512-20. 2008
    ..This may provide a molecular explanation for the clinically observed hyperreactivity of platelets in high-risk patients and serve as a basis for novel therapeutic interventions...
  7. doi Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation
    Francesc X Guix
    Laboratory of Molecular Physiology and Channelopathies, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain
    Brain 132:1335-45. 2009
    ....
  8. ncbi A computational model for free radicals transport in the microcirculation
    Mahendra Kavdia
    Biomedical Engineering Program, University of Arkansas, Fayetteville, 72701, USA
    Antioxid Redox Signal 8:1103-11. 2006
    ..The results will have significant implications for our understanding of these free radical interactions in physiologic and pathophysiologic conditions resulting from endothelial dysfunction...
  9. doi Mechanistic studies on the reaction between cob(II)alamin and peroxynitrite: evidence for a dual role for cob(II)alamin as a scavenger of peroxynitrous acid and nitrogen dioxide
    Riya Mukherjee
    Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA
    Chemistry 17:11805-12. 2011
    Peroxynitrite/peroxynitrous acid (ONOO(-)/ONOOH; pK(a(ONOOH)) =6.8) is implicated in multiple chronic inflammatory and neurodegenerative diseases. Both mammalian B(12)-dependent enzymes are inactivated under oxidative stress conditions...
  10. ncbi Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure
    Ivana Serraino
    Dipartimento Clinico e Sperimentale di Medicina e Farmacologia, Torre Biologica, Policlinico Universitario, 98123 Messina, Italy
    Life Sci 73:1097-114. 2003
    ....
  11. ncbi Peroxynitrite is an essential component of cytokines production mechanism in human monocytes through modulation of nuclear factor-kappa B DNA binding activity
    Bashir M Matata
    Department of Integrative Human Cardiovascular Physiology and Functional Genomics, Cardiac Surgery, University of Leicester, Glenfield Hospital, Groby Road, Leicester LE3 9QP, United Kingdom
    J Biol Chem 277:2330-5. 2002
    ..These findings indicate ONOO(-) plays an essential role in the mechanism of proinflammatory cytokine release by monocytes and that Rel/NF-kappaB activation is the obligatory pathway...
  12. ncbi Inactivation of NADP+-dependent isocitrate dehydrogenase by peroxynitrite. Implications for cytotoxicity and alcohol-induced liver injury
    Jin Hyup Lee
    Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702 701, Korea
    J Biol Chem 278:51360-71. 2003
    ..The peroxynitrite-mediated damage to ICDH may result in the perturbation of the cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition...
  13. ncbi Elevation of mitochondrial glutathione by gamma-glutamylcysteine ethyl ester protects mitochondria against peroxynitrite-induced oxidative stress
    Jennifer Drake
    Department of Chemistry and Center of Membrane Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
    J Neurosci Res 74:917-27. 2003
    ....
  14. ncbi The impact of metal catalysis on protein tyrosine nitration by peroxynitrite
    A Daiber
    Universitatsklinikum Hamburg Eppendorf, Medizinische Klinik III, Angiologie und Kardiologie, Martinistr 52, 20246 Hamburg, Germany
    Biochem Biophys Res Commun 317:873-81. 2004
    ..These results clearly identify peroxynitrite as the nitrating species even at low steady-state concentrations and demonstrate that metal catalysis plays an important role in nitration of protein-bound tyrosine...
  15. ncbi Reaction of astaxanthin with peroxynitrite
    Tsutomu Hayakawa
    Faculty of Agriculture, Shizuoka University, Shizuoka, Japan
    Biosci Biotechnol Biochem 72:2716-22. 2008
    ..Here we identify the major remaining reaction products of this reaction and investigate the stabilities of the nitro astaxanthins...
  16. doi SIN-1 cytotoxicity to PC12 cells is mediated by thiol-sensitive short-lived substances generated through SIN-1 decomposition in culture medium
    Kanako Konishi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, Japan
    Nitric Oxide 20:270-8. 2009
    ..These results suggest that SIN-1 cytotoxicity in PC12 cells is mediated via the generation of cytotoxic substances in the medium during its decomposition...
  17. pmc Uric acid protects against secondary damage after spinal cord injury
    Gwen S Scott
    Department of Microbiology and Immunology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 102:3483-8. 2005
    ..They also raise the possibility that elevating UA levels may provide a therapeutic approach for the treatment of SCI as well as other neurological diseases with a peroxynitrite-mediated pathological component...
  18. pmc Therapeutic intervention in experimental allergic encephalomyelitis by administration of uric acid precursors
    Gwen S Scott
    Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:16303-8. 2002
    ..The therapeutic effect on animals with active EAE was associated with increased UA, but not inosine, levels in CNS tissue. We, therefore, conclude that the mode of action of inosine and inosinic acid in EAE is via their metabolism to UA...
  19. ncbi Nitric oxide and nitroxidative stress in Alzheimer's disease
    Tadeusz Malinski
    Department of Chemistry and Biochemistry Ohio University, 350 W State Street, Athens, OH 45701, USA
    J Alzheimers Dis 11:207-18. 2007
    ..This article reviews the functions of NO and the potential role of NO/O2-/ONOO- induced nitroxidative stress in neuronal and endothelial degeneration observed in Alzheimer's disease...
  20. doi Neuroprotective effects of tempol, a catalytic scavenger of peroxynitrite-derived free radicals, in a mouse traumatic brain injury model
    Ying Deng-Bryant
    Spinal Cord and Brain Injury Research Center and Department of Anatomy and Neurobiology, University of Kentucky Chandler Medical Center, Lexington, Kentucky 40536 0509, USA
    J Cereb Blood Flow Metab 28:1114-26. 2008
    ..However, the modest neuroprotective effect of tempol suggests that multitarget combination strategies may be needed to interfere with posttraumatic secondary injury to a degree worthy of clinical translation...
  21. ncbi Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123
    Francisco Javier Martin-Romero
    Departamento de Bioquimica y Biologia Molecular y Genetica, Facultad de Ciencias and Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avenida de Elvas s n, 06080 Badajoz, Spain
    J Fluoresc 14:17-23. 2004
    ..It is shown that peroxynitrite concentration can be controlled (+/-5%) during predetermined times by using sequential SIN-1 pulses, to simulate chronic exposure of cells or subcellular components to peroxynitrite...
  22. pmc Quantitation of four guanine oxidation products from reaction of DNA with varying doses of peroxynitrite
    Hongbin Yu
    Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room 56 731A, Cambridge, Massachusetts 02139, USA
    Chem Res Toxicol 18:1849-57. 2005
    ..The complexity of these dose-response relationships is likely due to the dual role of peroxynitrite as both an oxidant and a nucleophile in competition with water...
  23. ncbi Contribution of peroxynitrite to fatal cardiovascular depression induced by overproduction of nitric oxide in rostral ventrolateral medulla of the rat
    S H H Chan
    Center for Neuroscience, National Sun Yat Sen University, 804, Kaohsiung, Taiwan, ROC
    Neuropharmacology 43:889-98. 2002
    ..We conclude that the formation of peroxynitrite by a reaction between superoxide anion and NO is primarily responsible for the fatal cardiovascular depression induced by overproduction of NO after activation of iNOS at the RVLM...
  24. ncbi Hydroxyl radical formation by O-O bond homolysis in peroxynitrous acid
    Sergei V Lymar
    Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
    Inorg Chem 42:5259-66. 2003
    ..These observations resolve major disputes over experimental data existing in the literature; despite extensive investigation of these reactions, no verifiable experimental evidence has been advanced that contradicts the homolysis model...
  25. pmc Chemical-biological fingerprinting: probing the properties of DNA lesions formed by peroxynitrite
    Sarah Delaney
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Chem Res Toxicol 20:1718-29. 2007
    ..Therefore, while 8-oxoGua itself is nontoxic and only mildly mutagenic in repair-proficient cells, peroxynitrite reveals the promutagenic potential and triggers the covert nature of this DNA lesion...
  26. ncbi Endothelial NADH/NADPH-dependent enzymatic sources of superoxide production: relationship to endothelial dysfunction
    Leszek Kalinowski
    Department of Clinical Chemistry and Laboratory of Cellular and Molecular Nephrology, Medical Research Centre of the Polish Academy of Science, Medical University of Gdansk, Poland
    Acta Biochim Pol 51:459-69. 2004
    ..It seems that O(2)(*-) generated by NAD(P)H oxidase may trigger eNOS uncoupling and contribute to the endothelial balance between NO and O(2)(*-). That is maintained at diverse levels...
  27. ncbi Peroxynitrite donor SIN-1 alters high-affinity choline transporter activity by modifying its intracellular trafficking
    Leah K Cuddy
    Molecular Brain Research Group, Robarts Research Institute, London, Ontario N6A 5K8, Canada
    J Neurosci 32:5573-84. 2012
    ..Increased oxidative-nitrosative stress in the microenvironment of cholinergic nerve terminals would diminish cholinergic transmission by reducing choline availability for ACh synthesis...
  28. ncbi Chronic production of peroxynitrite in the vascular wall impairs vasorelaxation function in SHR/NDmcr-cp rats, an animal model of metabolic syndrome
    Satomi Kagota
    Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women s University, Nishinomiya, Japan
    J Pharmacol Sci 109:556-64. 2009
    ....
  29. ncbi The apoprotein is the preferential target for peroxynitrite-induced LDL damage protection by dietary phenolic acids
    Teresa C P Dinis
    Laboratoire de Bioquímica Faculdade de Farmácia, Universidade de Coimbra, Portugal
    Free Radic Res 36:531-43. 2002
    ....
  30. doi Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation
    Eva Hrabarova
    Department of Carbohydrate Enzymology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    Gen Physiol Biophys 30:223-38. 2011
    ..Here we also describe our experimental set-up for studying peroxynitrite-mediated degradation of hyaluronan, a system, which may be suitable for testing prospective pharmacological substances...
  31. ncbi An inhibitor of inducible nitric oxide synthase and scavenger of peroxynitrite prevents diabetes development in NOD mice
    W L Suarez-Pinzon
    Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
    J Autoimmun 16:449-55. 2001
    ..These findings indicate that both superoxide and nitric oxide radicals contribute to islet beta-cell destruction in autoimmune diabetes via peroxynitrite formation in the beta-cells...
  32. doi Nitrocapsanthin and nitrofucoxanthin, respective products of capsanthin and fucoxanthin reaction with peroxynitrite
    Makoto Tsuboi
    Graduate of Science and Technology, Shizuoka University, 836 Ohya, Suruga ku, Shizuoka 422 8529, Japan
    J Agric Food Chem 59:10572-8. 2011
    ..Moreover, nitrocapsanthins (3 and 4) inhibited carcinogensis of mouse skin tumors initiated by 7,12-dimethylbenz[a]anthracene (DMBN)...
  33. doi BODIPY-based fluorescent probe for peroxynitrite detection and imaging in living cells
    Zhen Ning Sun
    Morningside Laboratory for Chemical Biology, Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, People s Republic of China
    Org Lett 11:1887-90. 2009
    ..This new probe will be a useful tool for studying the roles of peroxynitrite in biological processes...
  34. ncbi Mutation of tyrosine 190 to alanine eliminates the inactivation of cytochrome P450 2B1 by peroxynitrite
    Hsia lien Lin
    Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Chem Res Toxicol 16:129-36. 2003
    ..Modeling studies suggest that Tyr 190 may play a structural role in maintaining the integrity of the protein for maximal activity through hydrogen bonding with Glu 149...
  35. ncbi Effects of peroxynitrite dose and dose rate on DNA damage and mutation in the supF shuttle vector
    Min Young Kim
    Biological Engineering Division and Chemistry Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Chem Res Toxicol 18:76-86. 2005
    ..Furthermore, the results indicate that the chemistry of SIN-1-induced DNA damage differs substantially from native ONOO-, which suggests the need for caution in interpreting the biological relevance of SIN-1 as a surrogate for ONOO-...
  36. ncbi Tyrosine nitration of a synaptic protein synaptophysin contributes to amyloid beta-peptide-induced cholinergic dysfunction
    M H Tran
    Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Mol Psychiatry 8:407-12. 2003
    ..Our findings suggest that the tyrosine nitration of synaptophysin is related to Abeta-induced impairment of ACh release...
  37. pmc Neuroprotective efficacy and therapeutic time window of peroxynitrite decomposition catalysts in focal cerebral ischemia in rats
    Meenakshisundaram Thiyagarajan
    Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research NIPER, Sector 67, S A S Nagar, Mohali, Punjab, India
    Br J Pharmacol 142:899-911. 2004
    ....
  38. ncbi Rapid scavenging of peroxynitrous acid by monohydroascorbate
    Christophe R Kurz
    Laboratorium für Anorganische Chemie, Eidgenössische Technische Hochschule Zürich Hönggerberg, Zurich, Switzerland
    Free Radic Biol Med 35:1529-37. 2003
    The reaction of peroxynitrous acid with monohydroascorbate, over the concentration range of 250 microM to 50 mM of monohydroascorbate at pH 5...
  39. ncbi Peroxynitrite reactivity with amino acids and proteins
    B Alvarez
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Amino Acids 25:295-311. 2003
    ..The preferential protein targets of peroxynitrite and the role of proteins in peroxynitrite detoxifying pathways are discussed...
  40. ncbi Oxidative and nitrosative stress in acute renal ischemia
    E Noiri
    Department of Nephrology and Endocrinology, The University of Tokyo, Tokyo 113 8655, Japan 910 1193
    Am J Physiol Renal Physiol 281:F948-57. 2001
    ....
  41. pmc Role of nitrosative stress and peroxynitrite in the pathogenesis of diabetic complications. Emerging new therapeutical strategies
    Pal Pacher
    Laboratory of Physiologic Studies, National Institutes of Health, NIAAA, Bethesda, MD 20892 9413, USA
    Curr Med Chem 12:267-75. 2005
    ....
  42. ncbi Peroxynitrite mediates calcium-dependent mitochondrial dysfunction and cell death via activation of calpains
    Matthew Whiteman
    Department of Biochemistry, Faculty of Medicine, National University of Singapore, 8 Medical Dr, Republic of Singapore 117597
    FASEB J 18:1395-7. 2004
    ..Our data show, for the first time, that peroxynitrite induces mitochondrial dysfunction in cells via a calcium-dependent process that leads to caspase-independent apoptosis mediated by calpains...
  43. ncbi Reactive nitrogen species in the chemical biology of inflammation
    Peter C Dedon
    Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Arch Biochem Biophys 423:12-22. 2004
    ....
  44. ncbi Hyperlipidemia induced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts
    Annamaria Onody
    Cardiovascular Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, Dom ter 9, H 6720 Szeged, Hungary
    Cardiovasc Res 58:663-70. 2003
    ..We investigated the influence of experimental hyperlipidemia on the formation of cardiac NO, superoxide, and peroxynitrite (ONOO(-)) in rat hearts...
  45. ncbi Nitrogen dioxide and carbonate radical anion: two emerging radicals in biology
    Ohara Augusto
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil
    Free Radic Biol Med 32:841-59. 2002
    ..In this review, we provide a framework for this hypothesis and discuss the potential sources and properties of these radicals that are likely to become increasingly recognized as important mediators of biological processes...
  46. pmc Persistent eNOS activation secondary to caveolin-1 deficiency induces pulmonary hypertension in mice and humans through PKG nitration
    You Yang Zhao
    Department of Pharmacology and 2Center for Lung and Vascular Biology, University of Illinois College of Medicine, Chicago, Illinois, USA
    J Clin Invest 119:2009-18. 2009
    ..Thus, targeting of PKG nitration represents a potential novel therapeutic strategy for the treatment of PH...
  47. pmc Role of proinflammatory cytokines and redox homeostasis in exercise-induced delayed progression of hypertension in spontaneously hypertensive rats
    Deepmala Agarwal
    Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Dr, Baton Rouge, LA 70803, USA
    Hypertension 54:1393-400. 2009
    ....
  48. pmc Role of peroxynitrite in the redox regulation of cell signal transduction pathways
    Lucas Liaudet
    Department of Intensive Care Medicine, University Medical Center and Faculty of Biology and Medicine, Lausanne, Switzerland
    Front Biosci (Landmark Ed) 14:4809-14. 2009
    ....
  49. doi Peroxynitrite detoxification and its biologic implications
    Madia Trujillo
    Departamento de Bioquimica, Universidad de la Republica, Montevideo, Uruguay
    Antioxid Redox Signal 10:1607-20. 2008
    ..Flavonoids, nitroxides, and tyrosine-containing peptides decreased peroxynitrite-mediated toxicity under different conditions, but their mechanism of action is indirect...
  50. ncbi Scavenging of peroxynitrite reduces renal ischemia/reperfusion injury
    Ahmet Guven
    Department of Pediatric Surgery, Gulhane Military Medical Academy, Ankara, Turkey
    Ren Fail 30:747-54. 2008
    ....
  51. ncbi Neuronal NOS-mediated nitration and inactivation of manganese superoxide dismutase in brain after experimental and human brain injury
    Hulya Bayir
    Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania 15260, USA
    J Neurochem 101:168-81. 2007
    ..Nitration and inactivation of MnSOD could lead to self-amplification of oxidative stress in the brain progressively enhancing peroxynitrite production and secondary damage...
  52. ncbi Reperfusion-induced oxidative/nitrative injury to neurovascular unit after focal cerebral ischemia
    Yasemin Gursoy-OZdemir
    Department of Neurology, Faculty of Medicine, Institute of Neurological Sciences and Psychiatry, Hacettepe University, Ankara, Turkey
    Stroke 35:1449-53. 2004
    ..However, depending on their cellular origin and interactions between them, these molecules may exert protective or deleterious actions, neither of which is characterized in the intact brain...
  53. pmc Histone H1.2 is a substrate for denitrase, an activity that reduces nitrotyrosine immunoreactivity in proteins
    Yasuyuki Irie
    Department of Integrative Biology and Pharmacology and the Institute of Molecular Medicine, University of Texas Medical School, 6431 Fannin, MSB 4 100, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:5634-9. 2003
    ..The activity was shown to be specific for Histone H1.2, to differ from proteasome activity, and to require no additional cofactors...
  54. ncbi Superoxide radical- and peroxynitrite-scavenging activity of anthocyanins; structure-activity relationship and their synergism
    M Mamunur Rahman
    Niigata University of Pharmacy and Applied Life Sciences, Faculty of Applied Life Sciences, 265 1 Higashijima, Niigata, Japan
    Free Radic Res 40:993-1002. 2006
    ..It was confirmed that methylation of 4'-OH markedly reduced the antioxidant activity of anthocyanin. Further, it was revealed that synergism occurred in both - and ONOO- -scavenging activities among the anthocyanins in the mixture...
  55. pmc Redox regulation of the AMP-activated protein kinase
    Yingying Han
    Department of Biochemistry and Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, United States of America
    PLoS ONE 5:e15420. 2010
    ..Redox state is a critical determinant of cell function, and any major imbalances can cause severe damage or death...
  56. ncbi Peroxynitrite-mediated oxidative damage to brain mitochondria: Protective effects of peroxynitrite scavengers
    Indrapal N Singh
    Spinal Cord and Brain Injury Research Center, University of Kentucky College of Medicine, Lexington, KY 40536 0509, USA
    J Neurosci Res 85:2216-23. 2007
    ....
  57. ncbi Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase
    Timothy M Millar
    Xanthine Oxidase Research Group, School of Health, University of Bath, Claverton Down, Bath BA2 7AY, UK
    FEBS Lett 562:129-33. 2004
    ..Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity...
  58. ncbi Degradation of matrix glycosaminoglycans by peroxynitrite/peroxynitrous acid: evidence for a hydroxyl-radical-like mechanism
    Eleanor C Kennett
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 42:1278-89. 2007
    The oxidant peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) is generated at sites of inflammation via reaction of O2.- with .NO...
  59. ncbi Peroxynitrite mediates VEGF's angiogenic signal and function via a nitration-independent mechanism in endothelial cells
    A B El-Remessy
    Program in Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Athens, USA
    FASEB J 21:2528-39. 2007
    ..In conclusion, peroxynitrite is critically involved in transducing VEGF's angiogenic signal via nitration-independent and oxidation-mediated tyrosine phosphorylation...
  60. doi Early intervention of tyrosine nitration prevents vaso-obliteration and neovascularization in ischemic retinopathy
    Mohammed A Abdelsaid
    Program in Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA 30912, USA
    J Pharmacol Exp Ther 332:125-34. 2010
    ..Early inhibition of tyrosine nitration with use of epicatechin or NAC can represent safe and effective vascular-protective agents in ischemic retinopathy...
  61. ncbi Peroxynitrite decomposition catalysts prevent myocardial dysfunction and inflammation in endotoxemic rats
    Steve Lancel
    EA 2689, Universite de Lille 2, Faculte de Medecine, Lille, France
    J Am Coll Cardiol 43:2348-58. 2004
    ..The aim of this study was to test whether peroxynitrite neutralizers would reduce peroxynitrite accumulation and improve myocardial contractile dysfunction and inflammation in endotoxin-treated rats...
  62. pmc Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway
    Rosanna Vaschetto
    Department of Clinical and Experimental Medicine, University of Eastern Piedmont Amedeo Avogadro, Corso Mazzini 18, Novara, Italy
    Crit Care 14:R45. 2010
    ....
  63. ncbi Peroxynitrite mediates TNF-alpha-induced endothelial barrier dysfunction and nitration of actin
    Paul Neumann
    151, 113 Holland Ave, Dept of Veterans Affairs Medical Center, Albany, NY 12208, USA
    Am J Physiol Lung Cell Mol Physiol 290:L674-L684. 2006
    ..The data indicate that TNF induces an ONOO(-)-dependent barrier dysfunction, which is associated with the generation of NO(2)-beta-actin...
  64. ncbi Protective mechanisms of a metalloporphyrinic peroxynitrite decomposition catalyst, WW85, in rat cardiac transplants
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 314:53-60. 2005
    ..Rather, effects seem to be downstream on decreased protein nitration, decreased lipid peroxidation, and decreased PARP activation...
  65. ncbi Differential mechanisms of nitric oxide- and peroxynitrite-induced cell death
    Johanna T A Meij
    Department of Cell Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, OH 45267 0521, USA
    Mol Pharmacol 66:1043-53. 2004
    ..Moreover, post-treatment with insulin inhibited caspase-3 activation and loss of cell viability in NONOate- but not in SIN-1-exposed cells. These findings suggest that NO is a potent toxin independent of peroxynitrite formation...
  66. ncbi Activation of protein kinase C zeta by peroxynitrite regulates LKB1-dependent AMP-activated protein kinase in cultured endothelial cells
    Zhonglin Xie
    Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 281:6366-75. 2006
    ..Taken together, we conclude that PKCzeta can regulate AMPK activity by increasing the Ser428 phosphorylation of LKB1, resulting in association of LKB1 with AMPK and consequent AMPK Thr172 phosphorylation by LKB1...
  67. ncbi Role for nitrosative stress in diabetic neuropathy: evidence from studies with a peroxynitrite decomposition catalyst
    Irina G Obrosova
    Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    FASEB J 19:401-3. 2005
    ..This provides the rationale for development of agents counteracting peroxynitrite formation and promoting peroxynitrite decomposition, and their evaluation in DN...
  68. ncbi Reversible inhibition of mammalian glutamine synthetase by tyrosine nitration
    Boris Gorg
    Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich Heine University Dusseldorf, Moorenstrasse 5, D 40225 Dusseldorf, Germany
    FEBS Lett 581:84-90. 2007
    ..The data point to a potential regulation of GS activity by a reversible tyrosine nitration. High levels of oxidative stress may irreversibly damage and predispose the enzyme to proteasomal degradation...
  69. ncbi Peroxynitrite decomposition catalyst, FP15, and poly(ADP-ribose) polymerase inhibitor, PJ34, inhibit leukocyte entrapment in the retinal microcirculation of diabetic rats
    R Sugawara
    Department of Ophthalmology, Asahikawa Medical College, Midorogaoka Higashi 2 1 Asahikawa 078 8510, Japan
    Curr Eye Res 29:11-6. 2004
    ..We evaluated the effectiveness of the peroxynitrite decomposition catalyst, FP15, and the PARP inhibitor, PJ34, in the treatment of leukocyte entrapment in the retinal microcirculation of diabetic rats...
  70. ncbi ICAM-1 upregulation in the spinal cords of PLSJL mice with experimental allergic encephalomyelitis is dependent upon TNF-alpha production triggered by the loss of blood-brain barrier integrity
    Gwen S Scott
    Department of Microbiology and Immunology, Thomas Jefferson University, JAH 454, 1020 Locust Street, Philadelphia, PA 19107, USA
    J Neuroimmunol 155:32-42. 2004
    ....
  71. pmc Hemodynamics influences vascular peroxynitrite formation: Implication for low-density lipoprotein apo-B-100 nitration
    Tzung K Hsiai
    Department of Biomedical Engineering and Cardiovascular Medicine, University of Southern California, Los Angeles, CA 90081, USA
    Free Radic Biol Med 42:519-29. 2007
    ..Hence, the characteristics of shear stress in the arterial bifurcations influenced the relative production of O2(-.) and .NO with an implication for ONOO(-) formation as evidenced by LDL protein nitration...
  72. ncbi Human atherosclerotic intima and blood of patients with established coronary artery disease contain high density lipoprotein damaged by reactive nitrogen species
    Subramaniam Pennathur
    Department of Medicine, School of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:42977-83. 2004
    ..Thus, nitrated HDL might represent a previously unsuspected link between nitrosative stress, atherosclerosis, and inflammation...
  73. pmc Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells
    Ping Song
    Section of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, 73104, USA
    J Biol Chem 283:12446-55. 2008
    ..Finally, ONOO(-) enhanced PKCzeta nuclear import and LKB1 nuclear export. We conclude that PKCzeta mediates LKB1-dependent Akt inhibition in response to ONOO(-), resulting in endothelial apoptosis...
  74. ncbi Reduction of insulin-stimulated glucose uptake by peroxynitrite is concurrent with tyrosine nitration of insulin receptor substrate-1
    Takashi Nomiyama
    Department of Medicine, Metabolism and Endocrinology, Juntendo University, School of Medicine, Tokyo, Japan
    Biochem Biophys Res Commun 320:639-47. 2004
    ..Our results suggest that peroxynitrite reduces the IRS-1 protein level and decreases phosphorylation of IRS-1 concurrent with nitration of its tyrosine residues...
  75. ncbi Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging
    Shanqin Xu
    Vascular Biology Unit X708, Whitaker Cardiovascular Unit, Evans Department of Medicine, Boston University Medical Center, 650 Albany St, Boston, MA 02118, USA
    Am J Physiol Heart Circ Physiol 290:H2220-7. 2006
    ....
  76. ncbi Nitric oxide and its role in ischaemic brain injury
    Robert G Keynes
    Wolfson Institute for Biomedical Research, University College London, Cruciform Building, Gower Street, London WC1E 6BT, UK
    Curr Mol Med 4:179-91. 2004
    ..Better understanding of the molecular mechanisms by which NO is protective may ultimately identify new potential therapeutic targets...
  77. doi Role of oxidative stress in multiparity-induced endothelial dysfunction
    Huda E Tawfik
    Faculty of Medicine and Dentistry, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    Am J Physiol Heart Circ Physiol 295:H1736-42. 2008
    ..53+/-0.1 protein/actin vs. virgins: 1.0+/-0.14 protein/actin). These data suggest that multiparity induces endothelial dysfunction through decreased NO bioavailability and increased reactive oxygen species formation...
  78. doi The chemistry of peroxynitrite: implications for biological activity
    Sara Goldstein
    Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
    Methods Enzymol 436:49-61. 2008
    ....
  79. doi Peroxynitrite mediates retinal neurodegeneration by inhibiting nerve growth factor survival signaling in experimental and human diabetes
    Tayyeba K Ali
    Program in Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA 30912, USA
    Diabetes 57:889-98. 2008
    ..However, the causal role of peroxynitrite and the impact of tyrosine nitration on diabetes-induced retinal neurodegeneration and NGF survival signaling have not been elucidated...
  80. ncbi Chain scission of hyaluronan by peroxynitrite
    S Al-Assaf
    The North East Wales Institute, Plas Coch, Mold Road, LL11 2AW, Wrexham, UK
    Arch Biochem Biophys 411:73-82. 2003
    ..5% chain breaks (per peroxynitrite concentration). These conclusions support the proposal that the yield of hydroxyl radicals arising from the isomerization of ONOOH to nitrate ions is relatively low...
  81. ncbi Enteroinvasive bacteria alter barrier and transport properties of human intestinal epithelium: role of iNOS and COX-2
    Silvia Resta-Lenert
    Department of Medicine, and Biomedical Sciences Ph D Program, University of California, San Diego, School of Medicine, San Diego, California, USA
    Gastroenterology 122:1070-87. 2002
    ....
  82. pmc Part II: beneficial effects of the peroxynitrite decomposition catalyst FP15 in murine models of arthritis and colitis
    Jon G Mabley
    Inotek Pharmaceuticals Corporation, Cummings Center, Beverly, Massachusetts 01905, USA
    Mol Med 8:581-90. 2002
    ..Here, using a novel peroxynitrite decomposition catalyst, FP15, we directly investigate the role of peroxynitrite in the pathogenesis of arthritis and colitis in rodent models...
  83. pmc Part I: pathogenetic role of peroxynitrite in the development of diabetes and diabetic vascular complications: studies with FP15, a novel potent peroxynitrite decomposition catalyst
    Csaba Szabo
    Inotek Pharmaceuticals Corporation, Beverly, Massachusetts, USA
    Mol Med 8:571-80. 2002
    ..The aim of the current work was to produce a potent peroxynitrite decomposition catalyst and to test its effects in rodent models of diabetes and its complications...
  84. ncbi Aberrant expression of NOS isoforms in Alzheimer's disease is structurally related to nitrotyrosine formation
    Hans Joachim Lüth
    Paul Flechsig Institute of Brain Research, Department of Neuroanatomy, University of Leipzig, Jahnallee 59, D 04109 Leipzig, Germany
    Brain Res 953:135-43. 2002
    ..In view of the wide range of isoform-specific NOS inhibitors, the determination of the most responsible isoform of NOS for the formation of peroxynitrite in AD could be of therapeutic importance in the treatment of Alzheimer's disease...
  85. ncbi Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid
    Eleanor C Kennett
    The Heart Research Institute, 114 Pyrmont Bridge Road, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 45:716-25. 2008
    ..Peroxynitrite/peroxynitrous acid, which is generated at elevated levels at sites of inflammation, is believed to play a role in ECM damage; ..
  86. pmc Peroxynitrite decomposition catalyst prevents apoptotic cell death in a human astrocytoma cell line incubated with supernatants of HIV-infected macrophages
    Carolina Muscoli
    Faculty of Pharmacy, University of Catanzaro Magna Graecia, Roccelletta di Borgia, Catanzaro, Italy
    BMC Neurosci 3:13. 2002
    ..Here we investigated the role of peroxynitrite in apoptosis occurring in astroglial cells incubated with supernatants of HIV-infected human primary macrophages (M/M)...
  87. ncbi Increased nitric oxide-dependent nitrosylation of 4,5-diaminofluorescein by oxidants: implications for the measurement of intracellular nitric oxide
    DAVID JOURD'HEUIL
    Center for Cardiovascular Sciences, Albany Medical College, Albany, NY 12208, USA
    Free Radic Biol Med 33:676-84. 2002
    ..Our findings indicate that DAF-2 fluorometric assays are quantitatively difficult to interpret in cells and in solution when oxidants and (*) NO are co-generated...
  88. ncbi Modulation by peroxynitrite of Akt- and AMP-activated kinase-dependent Ser1179 phosphorylation of endothelial nitric oxide synthase
    Ming Hui Zou
    Vascular Biology Unit, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:32552-7. 2002
    ..We conclude that ONOO(-) inhibits Akt and increases AMPK-dependent Ser(1179) phosphorylation of eNOS resulting in enhanced O(2)() release...
  89. pmc Experimental diabetes causes breakdown of the blood-retina barrier by a mechanism involving tyrosine nitration and increases in expression of vascular endothelial growth factor and urokinase plasminogen activator receptor
    Azza B el-Remessy
    Vascular Biology Center, Medical College of Georgia, Augusta, Georgia 30909, USA
    Am J Pathol 162:1995-2004. 2003
    ..Taken together, these data indicate that early diabetes causes breakdown of the BRB by a mechanism involving the action of reactive nitrogen species in promoting expression of VEGF and uPAR...
  90. pmc Evaluation of the peroxynitrite decomposition catalyst Fe(III) tetra-mesitylporphyrin octasulfonate on peripheral neuropathy in a mouse model of type 1 diabetes
    Viktor R Drel
    Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    Int J Mol Med 20:783-92. 2007
    ..The findings provide rationale for development of potent peroxynitrite decomposition catalysts for the treatment of diabetic neuropathy...
  91. pmc Bicarbonate enhances alpha-synuclein oligomerization and nitration: intermediacy of carbonate radical anion and nitrogen dioxide radical
    Christopher Andrekopoulos
    Department of Biophysics, Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, P O Box 26509, Milwaukee, WI 53226, USA
    Biochem J 378:435-47. 2004
    ..Hydrophobic membranes greatly influenced alpha-synuclein aggregation and nitration in these systems. We conclude that both CO3*- and NO2* could play a major role in the nitration/aggregation of alpha-synuclein...
  92. doi Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation
    Eleanor C Kennett
    The Heart Research Institute, Camperdown, NSW 2050, Australia
    Free Radic Biol Med 47:389-400. 2009
    ..The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation...
  93. pmc Stimulation of peroxynitrite catalysis improves insulin sensitivity in high fat diet-fed mice
    Herve Duplain
    Department of Internal Medicine and Botnar Center for Clinical Research, University Hospital Center and University of Lausanne, Switzerland
    J Physiol 586:4011-6. 2008
    ..Stimulation of peroxynitrite catalysis attenuates HFD-induced insulin resistance in mice by restoring insulin signalling and insulin-stimulated glucose uptake in skeletal muscle tissue...
  94. pmc Neuroprotective effect of (-)Delta9-tetrahydrocannabinol and cannabidiol in N-methyl-D-aspartate-induced retinal neurotoxicity: involvement of peroxynitrite
    Azza B el-Remessy
    Department of Pharmacology and Toxicology, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912, USA
    Am J Pathol 163:1997-2008. 2003
    ..The effect of THC was in part mediated by the cannabinoid receptor CB1. These results suggest the potential use of CBD as a novel topical therapy for the treatment of glaucoma...
  95. ncbi Roles of superoxide, peroxynitrite, and protein kinase C in the development of tolerance to nitroglycerin
    G Abou-Mohamed
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Pharmacol Exp Ther 308:289-99. 2004
    ..In conclusion, tolerance to GTN involves enhanced production of O2*- and ONOO- and activation of NO synthase. Furthermore, sustained activation of alpha- and epsilonPKC isozymes in EC by GTN may play a role in development of tolerance...
  96. ncbi Enhanced mitochondrial superoxide in hyperglycemic endothelial cells: direct measurements and formation of hydrogen peroxide and peroxynitrite
    Celia Quijano
    Departamento de Bioquimica, Facultad de Medicina, Avenida General Flores 2125, 11800 Montevideo, Uruguay
    Am J Physiol Heart Circ Physiol 293:H3404-14. 2007
    ..Mitochondrial O(2)(.-) formation in hyperglycemic conditions was associated with increased glucose metabolization in the Krebs cycle and hyperpolarization of the mitochondrial membrane...
  97. pmc Identification of the serine 307 of LKB1 as a novel phosphorylation site essential for its nucleocytoplasmic transport and endothelial cell angiogenesis
    Zhonglin Xie
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Mol Cell Biol 29:3582-96. 2009
    ....
  98. ncbi Activation of microsomal glutathione s-transferase by peroxynitrite
    Yanbin Ji
    Department of Pharmacology and Toxicology, Faculty of Health Sciences, Queen s University, Kingston, Ontario, Canada
    Mol Pharmacol 63:136-46. 2003
    ....
  99. ncbi Proteomic identification of nitrated proteins in Alzheimer's disease brain
    Alessandra Castegna
    Department of Chemistry, Center of Membrane Sciences, University of Kentucky, Lexington, Kentucky 40506 0055, USA
    J Neurochem 85:1394-401. 2003
    ....
  100. ncbi Peroxynitrite induces apoptosis in rat aortic smooth muscle cells: possible relation to vascular diseases
    Jianfeng Li
    Department of Physiology, State University of New York, Downstate Medical Center, Brooklyn, New York 11203, USA
    Exp Biol Med (Maywood) 229:264-9. 2004
    ....
  101. pmc Role of peroxynitrite in the pathogenesis of cardiovascular complications of diabetes
    Pal Pacher
    National Institutes of Health, NIAAA, Laboratory of Physiologic Studies, 5625 Fishers Lane MSC 9413, Bethesda, Maryland 20852, USA
    Curr Opin Pharmacol 6:136-41. 2006
    ....

Research Grants110 found, 100 shown here

  1. CHEMICAL REACTIVITY OF PEROXYNITRITE
    WILLEM KOPPENOL; Fiscal Year: 1992
    ..These pathways may involve the hydroxyl radical, OH (from homolysis of the peroxide bond in peroxynitrous acid), the nitronium cation, NO2+ (from heterolysis), or peroxynitrite itself...
  2. Molecular Basis for Microbicidal Action in Phagocytes
    James Hurst; Fiscal Year: 2003
    ..enzyme, myeloperoxidase, and the microbicidal competence of the putative macrophage-generated toxin, peroxynitrous acid (ONOOH)...
  3. Molecular Basis for Microbicidal Action in Phagocytes
    James Hurst; Fiscal Year: 2004
    ..enzyme, myeloperoxidase, and the microbicidal competence of the putative macrophage-generated toxin, peroxynitrous acid (ONOOH)...
  4. Molecular Basis for Microbicidal Action in Phagocytes
    James Hurst; Fiscal Year: 2005
    ..enzyme, myeloperoxidase, and the microbicidal competence of the putative macrophage-generated toxin, peroxynitrous acid (ONOOH)...
  5. Molecular Basis for Microbicidal Action in Phagocytes
    James Hurst; Fiscal Year: 2001
    ..enzyme, myeloperoxidase, and the microbicidal competence of the putative macrophage-generated toxin, peroxynitrous acid (ONOOH)...
  6. Molecular Basis for Microbicidal Action in Phagocytes
    James Hurst; Fiscal Year: 2002
    ..enzyme, myeloperoxidase, and the microbicidal competence of the putative macrophage-generated toxin, peroxynitrous acid (ONOOH)...
  7. NITRIC OXIDE TOXICITY--OXIDATIONS VIA PEROXYNITRITE
    WILLIAM PRYOR; Fiscal Year: 1999
    ..Nitric oxide itself is a weak oxidant, but it reacts with O2-. in the lung to produce peroxynitrite and peroxynitrous acid. HOONO, potent oxidants and toxins...
  8. PEROXYNITRITE DAMAGE TO MICROTUBULE PROTEINS
    LISA LANDINO; Fiscal Year: 2007
    ..Researchers must consider and assess the effects of reactive molecules on abundant brain proteins because it is very likely that many proteins are damaged as we age. [unreadable] [unreadable]..
  9. Methamphetamine Neurotoxicity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2008
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  10. POLY (ADP-RIBOSE) SYNTHETASE AND REPERFUSION INJURY
    Csaba Szabo; Fiscal Year: 1999
    ..The principal investigator will also investigate the changes in endothelial function during myocardial reperfusion injury, and the role of PARS in these changes. ..
  11. Myicardial injury: role of poly (ADP-ribose) synthetase
    Csaba Szabo; Fiscal Year: 2004
    ..Further, based on exciting preliminary data, the final, fifth aim is focusing in establishing the role of PARS in myocardial preconditioning. ..
  12. Adenosine-3 agonists for the suppression of arthritis
    Csaba Szabo; Fiscal Year: 2003
    ..A novel A3 receptor agonist may represent a useful anti-inflammatory adjunct to current therapeutic regimens; funding of the current SBIR Phase II will allow for starting human safety trials in 2 years. ..
  13. Novel preservation fluid for organ transplantation
    Jon Mabley; Fiscal Year: 2003
    ..abstract_text> ..
  14. Peroxynitrate neutralizing agents for stroke therapy
    Csaba Szabo; Fiscal Year: 2003
    ....
  15. PARS and peroxynitrite induced cell death
    Csaba Szabo; Fiscal Year: 2007
    ..4 To establish the role of Bel family proteins in the regulation of calcium homeostasis during peroxynitrite-induced cell death. The studies proposed here have broad pathophysiological relevance. ..
  16. Ultrapotent PARS inhibitor for CNS trauma
    Csaba Szabo; Fiscal Year: 2003
    ..Estimated worldwide revenues after market entry and maturation (ca. 4 years after FDA approval)=$750 million (annual). ..
  17. Mechanisms of cardiovascular dysfunction in diabetes
    Csaba Szabo; Fiscal Year: 2003
    ..The final aim of the study is to investigate the activation of PARP in human microvasculature, and to correlate the degree of PARP activation with the degree of endothelial dysfunction in humans. ..
  18. NEUROTOXIC AMPHETAMINES RADICAL & 5HT NEURONS
    DONALD KUHN; Fiscal Year: 2003
    ..These studies will test novel hypotheses relating to the identification of reactive species that mediate METH and MDMA effects on monoamine nerve endings. ..
  19. Role of peroxynitrite in morphine hyperalgesia and tolerance
    Daniela Salvemini; Fiscal Year: 2008
    ....
  20. Role of ceramide in morphine hyperalgesia and tolerance
    Daniela Salvemini; Fiscal Year: 2008
    ..The broader implications of our findings may open a new frontier in chronic pain management thus alleviating the socioeconomic consequences it causes. ..
  21. NEUROTOXIC AMPHETAMINES RADICAL & 5HT NEURONS
    DONALD KUHN; Fiscal Year: 2004
    ..These studies will test novel hypotheses relating to the identification of reactive species that mediate METH and MDMA effects on monoamine nerve endings. ..
  22. NEUROSCIENCE TRAINING IN DRUG ABUSE
    DONALD KUHN; Fiscal Year: 2004
    ..abstract_text> ..
  23. Methamphetamine Neurotoxicity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2007
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  24. Neurotoxic Amphetamines Radicals & 5HT Neurons
    DONALD KUHN; Fiscal Year: 2007
    ..Research in this proposal is especially relevant to AIDS- related neuropathology, Parkinson's disease, and other neurodegenerative conditions in which microglial activation contributes to CNS damage. ..
  25. Mechanisms of cardiovascular dysfunction in diabetes
    Csaba Szabo; Fiscal Year: 2005
    ..The final aim of the study is to investigate the activation of PARP in human microvasculature, and to correlate the degree of PARP activation with the degree of endothelial dysfunction in humans. ..
  26. PARS and peroxynitrite induced cell death
    Csaba Szabo; Fiscal Year: 2006
    ..4 To establish the role of Bel family proteins in the regulation of calcium homeostasis during peroxynitrite-induced cell death. The studies proposed here have broad pathophysiological relevance. ..
  27. Molecular Neurobiology of Drug Abuse
    DONALD KUHN; Fiscal Year: 2006
    ..Kuhn will remain extensively engaged in scientific mentoring activities. ..
  28. Methamphetamine Neurotoxity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2006
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  29. Methamphetamine Neurotoxicity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2005
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  30. PARS and peroxynitrite induced cell death
    Csaba Szabo; Fiscal Year: 2008
    ..4 To establish the role of Bel family proteins in the regulation of calcium homeostasis during peroxynitrite-induced cell death. The studies proposed here have broad pathophysiological relevance. ..
  31. Poly(ADP-ribose)and diabetic erectile dysfunction
    FANGMING ZUO; Fiscal Year: 2004
    ..If these studies confirm that Inotek's PARP inhibitor is effective in improving erectile function in diabetes, a Phase II component will be triggered, which will focus on formal preclinical safety testing. ..
  32. Myicardial injury: role of poly (ADP-ribose) synthetase
    Csaba Szabo; Fiscal Year: 2005
    ..Further, based on exciting preliminary data, the final, fifth aim is focusing in establishing the role of PARS in myocardial preconditioning. ..
  33. PARS INHIBITOR FOR THE THERAPY OF CIRCULATORY SHOCK
    Ludmila Belayev; Fiscal Year: 2003
    ..The current project will enable the applicants to move forward in two years, near to the level of the beginning of Phase II efficacy trial in septic patients. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  34. Mechanisms of cardiovascular dysfunction in diabetes
    Csaba Szabo; Fiscal Year: 2004
    ..The final aim of the study is to investigate the activation of PARP in human microvasculature, and to correlate the degree of PARP activation with the degree of endothelial dysfunction in humans. ..
  35. Myicardial injury: role of poly (ADP-ribose) synthetase
    Csaba Szabo; Fiscal Year: 2003
    ..Further, based on exciting preliminary data, the final, fifth aim is focusing in establishing the role of PARS in myocardial preconditioning. ..
  36. Poly(ADP-ribose) synthetase inhibition and diabetes
    Csaba Szabo; Fiscal Year: 2004
    ....
  37. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2000
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
  38. NEUROTOXIC AMPHETAMINES RADICAL & 5HT NEURONS
    DONALD KUHN; Fiscal Year: 2001
    ..These studies will test novel hypotheses relating to the identification of reactive species that mediate METH and MDMA effects on monoamine nerve endings. ..
  39. Role of peroxynitrite in morphine hyperalgesia and tolerance
    Daniela Salvemini; Fiscal Year: 2009
    ....
  40. PKC SIGNALING AND THE TREATMENT OF BIPOLAR DISORDER
    DONALD KUHN; Fiscal Year: 2000
    ....
  41. PARS and peroxynitrite induced cell death
    Csaba Szabo; Fiscal Year: 2009
    ..4 To establish the role of Bel family proteins in the regulation of calcium homeostasis during peroxynitrite-induced cell death. The studies proposed here have broad pathophysiological relevance. ..
  42. Neurotoxic Amphetamines Radicals & 5HT Neurons
    DONALD KUHN; Fiscal Year: 2008
    ..Research in this proposal is especially relevant to AIDS- related neuropathology, Parkinson's disease, and other neurodegenerative conditions in which microglial activation contributes to CNS damage. ..
  43. Neurotoxic Amphetamines Radicals & 5HT Neurons
    Donald M Kuhn; Fiscal Year: 2010
    ..Research in this proposal is especially relevant to AIDS- related neuropathology, Parkinson's disease, and other neurodegenerative conditions in which microglial activation contributes to CNS damage. ..
  44. Role of peroxynitrite in morphine hyperalgesia and tolerance
    Daniela Salvemini; Fiscal Year: 2010
    ....
  45. POLY (ADP-RIBOSE) SYNTHETASE AND REPERFUSION INJURY
    Csaba Szabo; Fiscal Year: 2000
    ..The principal investigator will also investigate the changes in endothelial function during myocardial reperfusion injury, and the role of PARS in these changes. ..
  46. NEUROTOXIC AMPHETAMINES RADICALS AND 5HT NEURONS
    DONALD KUHN; Fiscal Year: 1999
    ..Increases in brain levels of BH4 will test the hypothesis that the TPH cofactor can modulate the in vivo inactivation of TPH caused by MDMA as it does in vitro with NO/ROS. ..
  47. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2001
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
  48. Neurotoxic Amphetamines Radicals & 5HT Neurons
    DONALD KUHN; Fiscal Year: 2009
    ..Research in this proposal is especially relevant to AIDS- related neuropathology, Parkinson's disease, and other neurodegenerative conditions in which microglial activation contributes to CNS damage. ..
  49. PKC SIGNALING AND THE TREATMENT OF BIPOLAR DISORDER
    DONALD KUHN; Fiscal Year: 2002
    ....
  50. NEUROTOXIC AMPHETAMINES RADICAL & 5HT NEURONS
    DONALD KUHN; Fiscal Year: 2002
    ..These studies will test novel hypotheses relating to the identification of reactive species that mediate METH and MDMA effects on monoamine nerve endings. ..
  51. Poly(ADP-ribose)Polymerase & Doxorubicin Cardiotoxicity
    Jon Mabley; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: The annual anticipated revenues for an effective therapeutic to prevent anti-cancer-drug induced cardiodepression is over $100 million in the US alone. ..
  52. Methamphetamine Neurotoxicity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2009
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  53. Myicardial injury: role of poly (ADP-ribose) synthetase
    Csaba Szabo; Fiscal Year: 2002
    ..Further, based on exciting preliminary data, the final, fifth aim is focusing in establishing the role of PARS in myocardial preconditioning. ..
  54. PKC SIGNALING AND THE TREATMENT OF BIPOLAR DISORDER
    DONALD KUHN; Fiscal Year: 2001
    ....
  55. Mechanisms of cardiovascular dysfunction in diabetes
    Csaba Szabo; Fiscal Year: 2002
    ..The final aim of the study is to investigate the activation of PARP in human microvasculature, and to correlate the degree of PARP activation with the degree of endothelial dysfunction in humans. ..
  56. Doxorubicin cardiotoxicity:protection by peroxynitrite
    Csaba Szabo; Fiscal Year: 2002
    ..abstract_text> ..
  57. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2002
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..
  58. Novel Neuroprotectants for TBI
    Edward Hall; Fiscal Year: 2008
    ..Thousands experience long lasting brain injury that interferes with their ability to lead productive and full lives. We plan to test a promising new class of therapeutics for traumatic brain injury. [unreadable] [unreadable] [unreadable]..
  59. Role of poly(ADP-ribosyl)ation in diabetic neuropathy
    IRINA OBROSOVA; Fiscal Year: 2003
    ..The studies are of direct relevance to the pathogenesis of PDN and may provide the rationale for development of PARS inhibitors for its prevention and treatment. ..
  60. 12/15-Lipoxygenase and Diabetic Neuropathy
    IRINA OBROSOVA; Fiscal Year: 2008
    ..The findings will generate new information on the role for 12/15-LO in PDN of Type 1 and Type 2 diabetes, and may provide rationale for development of 12/15-LO inhibitors for its prevention and treatment. ..
  61. Peroxynitrite decomposition catalyst:diabetic neuropathy
    IRINA OBROSOVA; Fiscal Year: 2006
    ..abstract_text> ..
  62. High glucose and arteriolar Kv channels
    Yanping Liu; Fiscal Year: 2004
    ..The results may suggest new therapeutic approaches for the treatment of microvascular dysfunction in patients with diabetes. ..
  63. Elucidation of nitric oxide resistance mechanisms in motor neurons
    Amy Bishop; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  64. Reactive Nitrogen and Accelerated Atherosclerosis in Type I Diabetes
    Ming Hui Zou; Fiscal Year: 2008
    ..These studies will provide novel information as to how the metabolic stresses associated with diabetes cause damage to the endothelium. ..
  65. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2008
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  66. Role of Endothelin System and NAD(P)H Oxidase in Retinal Arteriolar Dysfunction
    TRAVIS HEIN; Fiscal Year: 2009
    ....
  67. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2010
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  68. MECHANISTIC PROBES FOR ENZYME-CATALYZED OXIDATIONS
    Martin Newcomb; Fiscal Year: 2008
    ....
  69. Reactive Nitrogen and Accelerated Atherosclerosis in Type I Diabetes
    Ming Hui Zou; Fiscal Year: 2009
    ..These studies will provide novel information as to how the metabolic stresses associated with diabetes cause damage to the endothelium. ..
  70. Ethanol Effects on Hepatic Proteolytic Systems
    TIANA CURRY MCCOY; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  71. Role of Endothelin System and NAD(P)H Oxidase in Retinal Arteriolar Dysfunction
    TRAVIS HEIN; Fiscal Year: 2009
    ..Project Description Page 6 ..
  72. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2009
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  73. MECHANISTIC PROBES FOR ENZYME-CATALYZED OXIDATIONS
    Martin Newcomb; Fiscal Year: 2007
    ....
  74. MECHANISTIC PROBES FOR ENZYME-CATALYZED OXIDATIONS
    Martin Newcomb; Fiscal Year: 2002
    ..Probes for mononuclear, non-heme iron enzymes are in early development, and the objective in the current research period will be the design of probes that are substrates for the enzymes of interest such as clavimanate synthase. ..
  75. Oxidant stress and diabetic endothelial dysfunction
    Ming Hui Zou; Fiscal Year: 2009
    ..abstract_text> ..
  76. DNA RADICAL KINETICS
    Martin Newcomb; Fiscal Year: 2001
    ....
  77. MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTS
    GALEN PIEPER; Fiscal Year: 2002
    ..These studies will provide a novel mechanism to explain the pathogenesis of dyskinesis and graft failure during cardiac transplant rejection. ..
  78. Model of Transport in Renal Medullary Microvasculature
    AURELIE EDWARDS; Fiscal Year: 2004
    ..abstract_text> ..
  79. Model of Transport in Renal Medullary Microvasculature
    AURELIE EDWARDS; Fiscal Year: 2005
    ..abstract_text> ..
  80. Reactive nitrogen species & accelerated atherosclerosis
    Ming Hui Zou; Fiscal Year: 2005
    ....
  81. Model of Transport in Renal Medullary Microvasculature
    AURELIE EDWARDS; Fiscal Year: 2006
    ..abstract_text> ..
  82. AMP-activated kinase in diabetic complications
    Ming Hui Zou; Fiscal Year: 2007
    ..They should also yield insights into how endothelium attempts to protect itself against these stresses and whether AMPK is a potential target for therapy for diabetes. ..
  83. Reactive nitrogen species & accelerated atherosclerosis
    Ming Hui Zou; Fiscal Year: 2006
    ....
  84. UV INDUCED AND PERK MEDIATED SIGNALING PATHWAYS
    Shiyong Wu; Fiscal Year: 2003
    ..The proposed research will also investigate the upstream and downstream signaling pathways that are related to UV-induced and PERK-mediated cell death. ..
  85. 12/15-Lipoxygenase and Diabetic Neuropathy
    IRINA OBROSOVA; Fiscal Year: 2009
    ..The findings will generate new information on the role for 12/15-LO in PDN of Type 1 and Type 2 diabetes, and may provide rationale for development of 12/15-LO inhibitors for its prevention and treatment. ..
  86. 12/15-Lipoxygenase and Diabetic Neuropathy
    IRINA OBROSOVA; Fiscal Year: 2007
    ..The findings will generate new information on the role for 12/15-LO in PDN of Type 1 and Type 2 diabetes, and may provide rationale for development of 12/15-LO inhibitors for its prevention and treatment. ..
  87. Biochemical Characterization of Opioid Receptors
    Gavril Pasternak; Fiscal Year: 2009
    ..Together, these studies should give a better understanding of the actions of opioids and their pharmacology. ..
  88. SYNTHESIS AND PHARMACOLOGY OF NOVEL OPIATE LIGANDS
    Gavril Pasternak; Fiscal Year: 1993
    ..Additional derivatives will be examined in vivo. Finally, we will compare the metabolism and pharmacokinetics of NalBzoH following both oral and intravenous administration...
  89. SYNTHESIS AND PHARMACOLOGY OF NOVEL OPIATE LIGANDS
    Gavril Pasternak; Fiscal Year: 1991
    ..Additional derivatives will be examined in vivo. Finally, we will compare the metabolism and pharmacokinetics of NalBzoH following both oral and intravenous administration...
  90. Oxidative Stress and Myocardial Remodeling Following MI
    Yao Sun; Fiscal Year: 2008
    ..abstract_text> ..
  91. Oxidative Stress and Myocardial Remodeling Following MI
    Yao Sun; Fiscal Year: 2007
    ..abstract_text> ..