hydroxyl radical

Summary

Summary: The univalent radical OH. Hydroxyl radical is a potent oxidizing agent.

Top Publications

  1. pmc Mistranslation of membrane proteins and two-component system activation trigger antibiotic-mediated cell death
    Michael A Kohanski
    Department of Biomedical Engineering, Center for BioDynamics, and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 135:679-90. 2008
  2. ncbi Hydroxyl radical mediates cisplatin-induced apoptosis in human hair follicle dermal papilla cells and keratinocytes through Bcl-2-dependent mechanism
    Sudjit Luanpitpong
    Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
    Apoptosis 16:769-82. 2011
  3. ncbi Hydroxyl radicals and DNA base damage
    J Cadet
    Departement de Recherche Fondamentale sur la Matiere Condensee, SCIB Laboratoire Lésions des Acides Nucléiques, CEA Grenoble, F 38054, Grenoble Cedex 9, France
    Mutat Res 424:9-21. 1999
  4. pmc Silver coordination polymers for prevention of implant infection: thiol interaction, impact on respiratory chain enzymes, and hydroxyl radical induction
    Oliver Gordon
    Infection Biology, Department of Biomedicine, University Hospital, CH 4056 Basel, Switzerland
    Antimicrob Agents Chemother 54:4208-18. 2010
  5. ncbi A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical
    Kaushik Biswas
    Department of Physiology, Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Kolkata 700 032, India
    J Biol Chem 278:10993-1001. 2003
  6. pmc Three-dimensional RNA structure refinement by hydroxyl radical probing
    Feng Ding
    Department of Biochemistry and Biophysics, University of North Carolina, USA
    Nat Methods 9:603-8. 2012
  7. pmc Hydroxyl radical and its scavengers in health and disease
    Boguslaw Lipinski
    Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
    Oxid Med Cell Longev 2011:809696. 2011
  8. pmc Rapid killing of Acinetobacter baumannii by polymyxins is mediated by a hydroxyl radical death pathway
    Timothy R Sampson
    Department of Microbiology and Immunology, Microbiology and Molecular Genetics Program, Emory University, Atlanta, Georgia, USA
    Antimicrob Agents Chemother 56:5642-9. 2012
  9. ncbi High-throughput SHAPE and hydroxyl radical analysis of RNA structure and ribonucleoprotein assembly
    Jennifer L McGinnis
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA
    Methods Enzymol 468:67-89. 2009
  10. ncbi Study on the hydroxyl radical scavenging activity changes of ginseng and ginsenoside-Rb2 by heat processing
    Ki Sung Kang
    Institute of Natural Medicine, University of Toyama, Japan
    Biol Pharm Bull 30:724-8. 2007

Detail Information

Publications396 found, 100 shown here

  1. pmc Mistranslation of membrane proteins and two-component system activation trigger antibiotic-mediated cell death
    Michael A Kohanski
    Department of Biomedical Engineering, Center for BioDynamics, and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 135:679-90. 2008
    ..and phenotypic analyses to provide insight into the pathway whereby aminoglycosides ultimately trigger hydroxyl radical formation...
  2. ncbi Hydroxyl radical mediates cisplatin-induced apoptosis in human hair follicle dermal papilla cells and keratinocytes through Bcl-2-dependent mechanism
    Sudjit Luanpitpong
    Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
    Apoptosis 16:769-82. 2011
    ..Studies using specific ROS scavengers further showed that hydroxyl radical, but not hydrogen peroxide or superoxide anion, is the primary oxidative species responsible for the ..
  3. ncbi Hydroxyl radicals and DNA base damage
    J Cadet
    Departement de Recherche Fondamentale sur la Matiere Condensee, SCIB Laboratoire Lésions des Acides Nucléiques, CEA Grenoble, F 38054, Grenoble Cedex 9, France
    Mutat Res 424:9-21. 1999
    ..Therefore, major efforts should be devoted to the reassessment of the level of oxidative base damage in cellular DNA using appropriate assays including suitable conditions of DNA extraction...
  4. pmc Silver coordination polymers for prevention of implant infection: thiol interaction, impact on respiratory chain enzymes, and hydroxyl radical induction
    Oliver Gordon
    Infection Biology, Department of Biomedicine, University Hospital, CH 4056 Basel, Switzerland
    Antimicrob Agents Chemother 54:4208-18. 2010
    ..enzymes by binding sulfhydryl (thiol) groups in amino acids and promote the release of iron with subsequent hydroxyl radical formation by an indirect mechanism likely mediated by reactive oxygen species...
  5. ncbi A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical
    Kaushik Biswas
    Department of Physiology, Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Kolkata 700 032, India
    J Biol Chem 278:10993-1001. 2003
    ..The severity of lesions correlates well with the increased level of endogenous hydroxyl radical (*OH), which when scavenged by dimethyl sulfoxide causes around 90% reduction of the lesions, indicating that ..
  6. pmc Three-dimensional RNA structure refinement by hydroxyl radical probing
    Feng Ding
    Department of Biochemistry and Biophysics, University of North Carolina, USA
    Nat Methods 9:603-8. 2012
    ..b>Hydroxyl radical probing (HRP), which is performed routinely in many laboratories, provides a measure of solvent accessibility ..
  7. pmc Hydroxyl radical and its scavengers in health and disease
    Boguslaw Lipinski
    Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
    Oxid Med Cell Longev 2011:809696. 2011
    ..However, beneficial effects of the great number of phytochemicals that are endowed with hydroxyl radical scavenging and/or iron chelating activities should not be considered as a proof for oxidative stress.
  8. pmc Rapid killing of Acinetobacter baumannii by polymyxins is mediated by a hydroxyl radical death pathway
    Timothy R Sampson
    Department of Microbiology and Immunology, Microbiology and Molecular Genetics Program, Emory University, Atlanta, Georgia, USA
    Antimicrob Agents Chemother 56:5642-9. 2012
    ..present an alternative mechanism based on data demonstrating that polymyxins induce rapid cell death through hydroxyl radical production...
  9. ncbi High-throughput SHAPE and hydroxyl radical analysis of RNA structure and ribonucleoprotein assembly
    Jennifer L McGinnis
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA
    Methods Enzymol 468:67-89. 2009
    ..guide for comprehensive and high-throughput analysis of RNA secondary and tertiary structure using SHAPE and hydroxyl radical footprinting...
  10. ncbi Study on the hydroxyl radical scavenging activity changes of ginseng and ginsenoside-Rb2 by heat processing
    Ki Sung Kang
    Institute of Natural Medicine, University of Toyama, Japan
    Biol Pharm Bull 30:724-8. 2007
    ..In this study, we investigated the hydroxyl radical (...
  11. ncbi Hydroxyl radical and superoxide dismutase in blood of patients with Parkinson's disease: relationship to clinical data
    Y Ihara
    Clinical Research Institute and Department of Neurology, National Minamiokayama Hospital, 4066 Hayashima Cho, Tsukubo Gun, Okayama, Japan
    J Neurol Sci 170:90-5. 1999
    b>Hydroxyl radical (...
  12. ncbi Kinetic analysis of superoxide anion radical-scavenging and hydroxyl radical-scavenging activities of platinum nanoparticles
    Takeki Hamasaki
    Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
    Langmuir 24:7354-64. 2008
    ..and hydroxyl radical (.OH)...
  13. ncbi Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: implications for the control of elongation growth
    P Schopfer
    Institut fur Biologie II der Universitat, Schänzlestr 1, D 79104 Freiburg, Germany
    Plant J 28:679-88. 2001
    ..It is concluded that OH fulfils basic criteria for a wall-loosening factor acting in auxin-mediated elongation growth of plant species with widely differing cell-wall polysaccharide compositions...
  14. pmc Hydroxyl radical modification of collagen type II increases its arthritogenicity and immunogenicity
    Uzma Shahab
    Department of Biochemistry, Faculty of Medicine, J N Medical College, Aligarh Muslim University, Aligarh, India
    PLoS ONE 7:e31199. 2012
    ..Collagen induced arthritis (CIA) in rodents (rats and mice) is an accepted experimental model for RA...
  15. ncbi Quinolinic acid-iron(ii) complexes: slow autoxidation, but enhanced hydroxyl radical production in the Fenton reaction
    J Platenik
    First Institute of Medical Chemistry and Biochemistry, First Faculty of Medicine, Charles University, Katerinska 32, CZ 12108 Prague 2, Czech Republic
    Free Radic Res 34:445-59. 2001
    ..In order to follow hydroxyl radical generation in the Fenton chemistry, electron paramagnetic resonance (EPR) spin trapping with 5,5-dimethyl-1-..
  16. pmc In vivo cell wall loosening by hydroxyl radicals during cress seed germination and elongation growth
    Kerstin Müller
    University of Freiburg, Faculty of Biology, Institute for Biology II, Botany Plant Physiology, D 79104 Freiburg, Germany
    Plant Physiol 150:1855-65. 2009
    ..Here, we report direct in vivo evidence for hydroxyl radical ((*)OH)-mediated cell wall loosening during plant seed germination and seedling growth...
  17. ncbi Effect of adenosine A(2A) receptor antagonists on L-DOPA-induced hydroxyl radical formation in rat striatum
    Krystyna Gołembiowska
    Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna Street, Kraków 31 343, Poland
    Neurotox Res 15:155-66. 2009
    ..ZM 241385) on L: -3,4-dihydroxyphenylalanine (L: -DOPA)-induced hydroxyl radical generation using in vivo microdialysis in the striatum of freely moving rats...
  18. ncbi Differential roles of hydrogen peroxide and hydroxyl radical in cisplatin-induced cell death in renal proximal tubular epithelial cells
    Su Mi Baek
    Department of Psychology, College of Medicine, Pusan National University, South Korea
    J Lab Clin Med 142:178-86. 2003
    ..that cisplatin-induced cell death in vitro could not be prevented by scavengers of hydrogen peroxide and hydroxyl radical in rabbit renal cortical slices...
  19. pmc Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibres
    R M Murphy
    Department of Zoology, La Trobe University, Melbourne 3086, Victoria, Australia
    J Physiol 586:2203-16. 2008
    ..The present findings help provide a mechanistic basis for diverse findings on the effects of ROS in muscle fibres and implicate OH(*) radicals and glutathione as likely mediators of the effects...
  20. pmc Lethal oxidative damage and mutagenesis are generated by iron in delta fur mutants of Escherichia coli: protective role of superoxide dismutase
    D Touati
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Universite Paris 7, France
    J Bacteriol 177:2305-14. 1995
    ..b>Hydroxyl radical scavengers dimethyl sulfoxide and thiourea also provided protection...
  21. ncbi Mapping nucleic acid structure by hydroxyl radical cleavage
    Thomas D Tullius
    Department of Chemistry, Boston University, Boston MA 02215, USA
    Curr Opin Chem Biol 9:127-34. 2005
    b>Hydroxyl radical footprinting is a widely used method for following the folding of RNA molecules in solution...
  22. ncbi Hydrogen-rich saline attenuates radiation-induced male germ cell loss in mice through reducing hydroxyl radicals
    Yunhai Chuai
    Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, People s Republic of China
    Biochem J 442:49-56. 2012
    ..Our results represent the first in vivo evidence in support of a radioprotective role of H2 by neutralizing •OH in irradiated tissue with no side effects...
  23. pmc Position of eukaryotic initiation factor eIF1 on the 40S ribosomal subunit determined by directed hydroxyl radical probing
    Ivan B Lomakin
    Department of Microbiology and Immunology, SUNY Downstate Medical Center Brooklyn, New York 11203, USA
    Genes Dev 17:2786-97. 2003
    ..eIF1 plays its discriminatory role, we determined its position on the 40S ribosomal subunit using directed hydroxyl radical cleavage...
  24. pmc Towards a unifying, systems biology understanding of large-scale cellular death and destruction caused by poorly liganded iron: Parkinson's, Huntington's, Alzheimer's, prions, bactericides, chemical toxicology and others as examples
    Douglas B Kell
    School of Chemistry and The Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK
    Arch Toxicol 84:825-89. 2010
    ..A variety of systems biology approaches, that I summarise, can predict both the mechanisms involved in these cell death pathways and the optimal sites of action for nutritional or pharmacological interventions...
  25. ncbi Mitochondrial aconitase is a source of hydroxyl radical. An electron spin resonance investigation
    J Vasquez-Vivar
    Department of Pathology, Cardiovascular Research Center, the Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 275:14064-9. 2000
    ..Consequently, the inactivation of m-aconitase by superoxide may increase the formation of hydroxyl radical ((*)OH) through the Fenton reaction in mitochondria...
  26. pmc Position of eukaryotic translation initiation factor eIF1A on the 40S ribosomal subunit mapped by directed hydroxyl radical probing
    Yingpu Yu
    Department of Microbiology and Immunology, SUNY Downstate Medical Center, NY, USA
    Nucleic Acids Res 37:5167-82. 2009
    ..Here, we determined the position of eIF1A on the 40S subunit by directed hydroxyl radical cleavage...
  27. ncbi Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and prevents in vivo ozone-induced tyrosine nitration in lungs
    Esaú Floriano-Sánchez
    Escuela Superior de Medicina del Instituto Politecnico Nacional, Plan de San Luis y Salvador Díaz Mirón S N, Col Casco de Santo Tomas, Mexico, Mexico
    Free Radic Res 40:523-33. 2006
    ..In this paper, it was evaluated the in vitro peroxynitrite (ONOO(-)), singlet oxygen ((1)O(2)), hydroxyl radical (OH(v)), hydrogen peroxide (H(2)O(2)), superoxide anion and hypochlorous acid (HOCl) scavenging capacity of ..
  28. ncbi The binding selectivity of ADAR2's dsRBMs contributes to RNA-editing selectivity
    Olen M Stephens
    Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA
    Chem Biol 11:1239-50. 2004
    ..Using directed hydroxyl radical cleavage data, molecular models were developed that predict important recognition surfaces on the RNA for ..
  29. pmc Future directions of structural mass spectrometry using hydroxyl radical footprinting
    Janna G Kiselar
    Center for Proteomics and Bioinformatics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    J Mass Spectrom 45:1373-82. 2010
    b>Hydroxyl radical protein footprinting coupled to mass spectrometry has been developed over the last decade and has matured to a powerful method for analyzing protein structure and dynamics...
  30. ncbi In vivo hydroxyl radical formation after quinolinic acid infusion into rat corpus striatum
    A Santamaria
    Departamento de Neuroquimica, Instituto Nacional de Neurologia y Neurocirugia Manuel Velasco Suarez, SSA Av Insurgentes Sur 3877, México D F 14269, Mexico
    Neuroreport 12:2693-6. 2001
    We studied the effect of an acute infusion of quinolinic acid (QUIN) on in vivo hydroxyl radical (.OH) formation in the striatum of awake rats. Using the microdialysis technique, the generation of...
  31. ncbi Prostaglandin release by spinal cord injury mediates production of hydroxyl radical, malondialdehyde and cell death: a site of the neuroprotective action of methylprednisolone
    D Liu
    Departments of Neurology and Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas, USA
    J Neurochem 77:1036-47. 2001
    ..3 times basal levels, respectively. This suggests that PGF(2alpha)-induced cell death is partly due to hydroxyl radical-triggered peroxidation...
  32. ncbi Concerted binding and bending of DNA by Escherichia coli integration host factor
    Gauri M Dhavan
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Mol Biol 315:1027-37. 2002
    ..includes sequential minor groove contacts that present strong, sequence specific protection patterns against hydroxyl radical cleavage...
  33. ncbi Rapid and specific detection of hydroxyl radical using an ultraweak chemiluminescence analyzer and a low-level chemiluminescence emitter: application to hydroxyl radical-scavenging ability of aqueous extracts of Food constituents
    C H Tsai
    Department of Food Nutrition, Chung-Hwa Institute of Technology, Tainan, Taiwan
    J Agric Food Chem 49:2137-41. 2001
    ..analyzer, it is possible to monitor the production of a specific oxygen-derived reactive species, such as hydroxyl radical ((*)OH), whenever a suitable chemiluminescent probe is obtainable...
  34. ncbi Paradoxical effects of adenosine receptor ligands on hydroxyl radical generation by L-DOPA in the rat striatum
    Krystyna Gołembiowska
    Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, Pl 31 343 Krakow, Poland
    Pharmacol Rep 60:319-30. 2008
    ..In our study, we investigated the role of adenosine A(1) and A(2A) receptor ligands in hydroxyl radical generation by L-DOPA in the rat striatum...
  35. ncbi The Escherichia coli GTPase ObgE modulates hydroxyl radical levels in response to DNA replication fork arrest
    Cyrielle I Kint
    Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium
    FEBS J 279:3692-704. 2012
    ..modules RelBE/MazEF, strains both of which have been shown to be involved in a pathway that stimulates hydroxyl radical formation following hydroxyurea treatment...
  36. ncbi Potential mechanism for pentachlorophenol-induced carcinogenicity: a novel mechanism for metal-independent production of hydroxyl radicals
    Ben Zhan Zhu
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P R China
    Chem Res Toxicol 22:969-77. 2009
    The hydroxyl radical ((*)OH) has been considered to be one of the most reactive oxygen species produced in biological systems. It has been shown that (*)OH can cause DNA, protein, and lipid oxidation...
  37. pmc Photolysis of hydrogen peroxide, an effective disinfection system via hydroxyl radical formation
    Hiroyo Ikai
    New Industry Creation Hatchery Center, Tohoku University, Aoba 6 6 10, Aramaki, Aoba ku, Sendai 980 8579, Japan
    Antimicrob Agents Chemother 54:5086-91. 2010
    ..Other results demonstrated that the bactericidal activity was dependent on the amount of hydroxyl radicals generated. Treatment of bacteria with 200 to 300 μM hydroxyl radicals would result in reductions of viable counts of >99.99%...
  38. ncbi Suppression of NF-κB signaling by andrographolide with a novel mechanism in human platelets: regulatory roles of the p38 MAPK-hydroxyl radical-ERK2 cascade
    Wan J Lu
    Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
    Biochem Pharmacol 84:914-24. 2012
    ..Furthermore, SB203580, but not BAY11-7082 or PD98059, reduced collagen-induced hydroxyl radical ((·)HO) formation...
  39. ncbi Hydroxyl radical formation via iron-mediated Fenton chemistry is inhibited by methylated catechols
    A J Nappi
    Department of Biology, Loyola University Chicago, IL 60626, USA
    Biochim Biophys Acta 1425:159-67. 1998
    ..Competitive side-chain methylation reactions may sustain or perpetuate some dihydroxyphenyls, creating an oxidatively less favorable environment for cells than would result from compounds formed by O-methylation...
  40. ncbi Hydroxyl radical-modified fibrinogen as a marker of thrombosis: the role of iron
    B Lipinski
    Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA
    Hematology 17:241-7. 2012
    ..The mechanism of this phenomenon is very likely based on hydroxyl radical-induced modification of fibrinogen tertiary structure with the formation of insoluble aggregates resistant to ..
  41. ncbi Hydroxyl radical-mediated modification of proteins as probes for structural proteomics
    Guozhong Xu
    Center for Proteomics, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA
    Chem Rev 107:3514-43. 2007
  42. pmc S-allylmercaptocysteine scavenges hydroxyl radical and singlet oxygen in vitro and attenuates gentamicin-induced oxidative and nitrosative stress and renal damage in vivo
    José Pedraza-Chaverri
    Departamento de Biologia, Universidad Nacional Autonoma de Mexico UNAM, Ciudad Universitaria, 04510, Mexico, d, F, Mexico
    BMC Clin Pharmacol 4:5. 2004
    ..In addition, the in vitro reactive oxygen species scavenging properties of S-allylmercaptocysteine were studied...
  43. pmc Molecular mechanism for metal-independent production of hydroxyl radicals by hydrogen peroxide and halogenated quinones
    Ben Zhan Zhu
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People s Republic of China
    Proc Natl Acad Sci U S A 104:17575-8. 2007
    ..This represents a mechanism of HO* production that does not require redox-active transition metal ions...
  44. ncbi Hydroxyl radical induced degradation of ibuprofen
    Erzsébet Illés
    Institute of Chemistry, Research Group of Environmental Chemistry, University of Szeged, Szeged, Hungary
    Sci Total Environ 447:286-92. 2013
    ..The toxic effect of the aerated ibuprofen solution first increased upon irradiation indicating a higher toxicity of the first degradation products, then decreased with increasing absorbed dose...
  45. ncbi Quantification of hydroxyl radical-derived oxidation products in peptides containing glycine, alanine, valine, and proline
    Philip E Morgan
    Free Radical Group, The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia
    Free Radic Biol Med 52:328-39. 2012
    ..Summation of the product concentrations provides clear evidence for the occurrence of chain reactions in peptides exposed to HO(•)/O(2), with the overall product yields exceeding that of the initial HO(•) generated...
  46. pmc Massive parallel-sequencing-based hydroxyl radical probing of RNA accessibility
    Lukasz Jan Kielpinski
    Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    Nucleic Acids Res 42:e70. 2014
    b>Hydroxyl Radical Footprinting (HRF) is a tried-and-tested method for analysis of the tertiary structure of RNA and for identification of protein footprints on RNA...
  47. ncbi The time course of hydroxyl radical formation following spinal cord injury: the possible role of the iron-catalyzed Haber-Weiss reaction
    Danxia Liu
    Department of Neurology, University of Texas Medical Branch, Galveston, 77555 0653, USA
    J Neurotrauma 21:805-16. 2004
    This study explores whether the hydroxyl radical (*OH)-one of the most destructive reactive oxygen species-plays a role in secondary spinal cord injury (SCI)...
  48. ncbi Hydroxyl radical formation by O-O bond homolysis in peroxynitrous acid
    Sergei V Lymar
    Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
    Inorg Chem 42:5259-66. 2003
    ..These observations resolve major disputes over experimental data existing in the literature; despite extensive investigation of these reactions, no verifiable experimental evidence has been advanced that contradicts the homolysis model...
  49. ncbi Redox characterization of usnic acid and its cytotoxic effect on human neuron-like cells (SH-SY5Y)
    Thallita Kelly Rabelo
    Laboratory of Pharmaceutical Assays and Toxicity, Federal University of Sergipe LeFT UFS, Sao Cristovao, SE, Brazil
    Toxicol In Vitro 26:304-14. 2012
    ..Moreover, our results suggest that potential neurotoxicological effects of UA should be further studied by additional approaches; for instance, in vivo and clinical studies...
  50. pmc Hydroxyl radical footprinting in vivo: mapping macromolecular structures with synchrotron radiation
    Tadepalli Adilakshmi
    T C Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Nucleic Acids Res 34:e64. 2006
    ..to those obtained in vitro and were consistent with the predicted accessibility of the RNA backbone to hydroxyl radical. Protection or enhanced cleavage of certain nucleotides in vivo can be explained by interactions with tRNA ..
  51. ncbi Some unusual nucleic acid bases are products of hydroxyl radical oxidation of DNA and RNA
    J Barciszewski
    Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznan
    Mol Biol Rep 26:231-8. 1999
    ..We identify them as primary or secondary products of the reaction of nucleic acids with hydroxyl radicals, the most active oxidising agent in the cell...
  52. ncbi The chemical and hydroxyl radical scavenging activity changes of ginsenoside-Rb1 by heat processing
    Yong Jae Lee
    Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Shillim Dong San 56 1, Seoul 151 742, Republic of Korea
    Bioorg Med Chem Lett 18:4515-20. 2008
    The chemical and hydroxyl radical (*OH) scavenging activity changes of ginsenoside Rb(1) (Rb(1)) by heat processing were investigated in this study...
  53. ncbi Hydroxyl radical generation by photosystem II
    Pavel Pospisil
    Service Bioénérgetique, Departement de Biologie Joliot Curie, CEA Saclay, F 91191 Gif sur Yvette, France
    Biochemistry 43:6783-92. 2004
    ..b>Hydroxyl radical generation was also lost in the absence of oxygen, whereas it was stimulated when the oxygen concentration ..
  54. ncbi Sensitive detection and localization of hydroxyl radical production in cucumber roots and Arabidopsis seedlings by spin trapping electron paramagnetic resonance spectroscopy
    Susannah Renew
    Institut fur Biologie II, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Plant J 44:342-7. 2005
    ..In particular, the analysis of hydroxyl radical (OH*) formation in biological reactions has rarely been attempted...
  55. ncbi Structural methods for studying IRES function
    Jeffrey S Kieft
    Department of Biochemistry and Molecular Genetics, University of Colorado at Denver, USA
    Methods Enzymol 430:333-71. 2007
    ..The protocols we present are solution hydroxyl radical probing, RNase T1 probing, native gel electrophoresis, sedimentation velocity analytical ultracentrifugation, ..
  56. ncbi Production of hydroxyl radical by the synergistic action of fungal laccase and aryl alcohol oxidase
    F Guillen
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    Arch Biochem Biophys 383:142-7. 2000
    A mechanism for the production of hydroxyl radical (*OH) during the oxidation of hydroquinones by laccase, the ligninolytic enzyme most widely distributed among white-rot fungi, has been demonstrated...
  57. ncbi Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen
    Ana Cipak
    Rudjer Boskovic Institute, Department of Molecular Medicine, Zagreb, Croatia
    Acta Biochim Pol 57:165-71. 2010
    ..cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive...
  58. pmc Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants
    H Li
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    EMBO J 15:1421-33. 1996
    Ethylation interference and hydroxyl radical footprinting were used to identify substrate ribose-phosphate backbone sites that interact with the Escherichia coli RNA processing enzyme, ribonuclease III...
  59. pmc Direct strand scission from a nucleobase radical in RNA
    Aaron C Jacobs
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    J Am Chem Soc 132:3668-9. 2010
    ..radicals are the major family of reactive intermediates produced in RNA exposed to diffusible species such as hydroxyl radical. The nucleobase radicals are believed to produce direct strand breaks by abstracting hydrogen atoms from ..
  60. pmc Effect of adenosine A(2A) receptor antagonists and L-DOPA on hydroxyl radical, glutamate and dopamine in the striatum of 6-OHDA-treated rats
    Krystyna Gołembiowska
    Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna Street, 31 343 Krakow, Poland
    Neurotox Res 21:222-30. 2012
    ..CSC) and 4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol (ZM 241385) on hydroxyl radical generation, and glutamate (GLU) and dopamine (DA) extracellular level using a microdialysis in the striatum ..
  61. ncbi Myricetin affords protection against peroxynitrite-mediated DNA damage and hydroxyl radical formation
    Wei Chen
    College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China
    Food Chem Toxicol 49:2439-44. 2011
    ..Furthermore, EPR spectroscopy demonstrated that the formation of DMPO-hydroxyl radical adduct (DMPO-OH) from peroxynitrite, and that myricetin inhibited the adduct signal in a concentration-..
  62. pmc The effect of adenosine A(2A) receptor antagonists on hydroxyl radical, dopamine, and glutamate in the striatum of rats with altered function of VMAT2
    Krystyna Gołembiowska
    Institute of Pharmacology, Polish Academy of Sciences, 31 343 Krakow, 12 Smetna Street, Krakow, Poland
    Neurotox Res 22:150-7. 2012
    ..ZM 241385) on extracellular DA, glutamate and hydroxyl radical formation was studied in the rat striatum using in vivo microdialysis...
  63. pmc Synergistic effect of thermal energy on bactericidal action of photolysis of H₂O₂ in relation to acceleration of hydroxyl radical generation
    Midori Shirato
    Division of Fixed Prosthodontics, Department of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Seiryou 4 1, Aoba ku, Sendai 980 8575, Japan
    Antimicrob Agents Chemother 56:295-301. 2012
    ..the present study is to evaluate the effect of thermal energy on the yield of and the bactericidal action of hydroxyl radical generated by photolysis of H(2)O(2)...
  64. ncbi Toxicity of iron and hydrogen peroxide: the Fenton reaction
    C C Winterbourn
    Department of Pathology, Christchurch School of Medicine, New Zealand
    Toxicol Lett 82:969-74. 1995
    ..The mechanism and how it is affected by different chelators, and the interpretation of results obtained in biological systems, are discussed...
  65. ncbi Gastroprotective effect of Neem (Azadirachta indica) bark extract: possible involvement of H(+)-K(+)-ATPase inhibition and scavenging of hydroxyl radical
    Uday Bandyopadhyay
    Department of Physiology, Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Kolkata 700032, India
    Life Sci 71:2845-65. 2002
    ..damage of the gastric mucosa by significantly blocking lipid peroxidation and by scavenging the endogenous hydroxyl radical ((z.rad;)OH)-the major causative factor for ulcer. The (z...
  66. ncbi Hydroxyl radical generation by electron paramagnetic resonance as a new method to monitor ambient particulate matter composition
    Tingming Shi
    Particle Research, Institut für Umweltmedizinische Forschung IUF at the University of Düsseldorf PO Box 10 30 45, 40021 Düsseldorf, Germany
    J Environ Monit 5:550-6. 2003
    ..The purpose of this study was to characterize hydroxyl radical (OH*) production by different size fractions of PM, to use as an alternative method for monitoring of PM ..
  67. ncbi Inhibitory activity of flavonoids from Prunus davidiana and other flavonoids on total ROS and hydroxyl radical generation
    Hyun Ah Jung
    Faculty of Food Science and Biotechnology, Pukyong National University, Busan 608 737, Korea
    Arch Pharm Res 26:809-15. 2003
    ..Also, the presence of an ortho-hydroxyl group, whether on the A-ring or B-ring, and a 3-hydroxyl group on the C-ring increased the inhibitory activity on both total ROS and *OH generation...
  68. ncbi Hyperbaric oxygenation alleviates MCAO-induced brain injury and reduces hydroxyl radical formation and glutamate release
    Zhong jin Yang
    Department of Anesthesiology, Upstate Medical University, Syracuse, NY 13210, USA
    Eur J Appl Physiol 108:513-22. 2010
    ..This study suggests that the decreased glutamate release and the reduced formation of hydroxyl free radicals might contribute to the neuroprotective effect of HBO...
  69. ncbi Hydroxyl radical generation, levels of tumor necrosis factor-alpha, and progression to heart failure after acute myocardial infarction
    Marco Valgimigli
    University of Ferrara, Ferrara, Italy
    J Am Coll Cardiol 43:2000-8. 2004
    We used acetylsalicylic acid (ASA) as a probing agent to quantify hydroxyl radical ((*)OH) in Controls and patients with coronary artery disease and to prospectively investigate (*)OH production in patients with myocardial infarction (MI) ..
  70. ncbi Phytic acid suppresses 1-methyl-4-phenylpyridinium ion-induced hydroxyl radical generation in rat striatum
    Toshio Obata
    Department of Pharmacology and Therapeutics, Oita Medical University, 1 1 Idaigaoka, Hasama, Oita 879 5593, Japan
    Brain Res 978:241-4. 2003
    The present study examined the antioxidant effect of phytic acid on iron (II)-enhanced hydroxyl radical (*OH) generation induced by 1-methyl-4-phenylpyridinium ion (MPP(+)) in the extracellular fluid of rat striatum...
  71. ncbi Oxidation reactions of cytosine DNA components by hydroxyl radical and one-electron oxidants in aerated aqueous solutions
    J Richard Wagner
    Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Acc Chem Res 43:564-71. 2010
    ..The data and insights presented here shed new light on oxidation reactions of cytosine and 5-methylcytosine and should facilitate their validation in cellular DNA...
  72. pmc Interaction of SmpB with ribosome from directed hydroxyl radical probing
    Daisuke Kurita
    Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036 8561, Japan
    Nucleic Acids Res 35:7248-55. 2007
    ..Here, we studied the sites and modes of binding of SmpB to the ribosome by directed hydroxyl radical probing from Fe(II) tethered to SmpB variants...
  73. ncbi Delayed neuronal death prevented by inhibition of increased hydroxyl radical formation in a transient cerebral ischemia
    T Yamamoto
    Pharmaceuticals Laboratory I, Yokohama Research Center, Mitsubishi Chemical Corp, Aoba ku, Yokohama, Japan
    Brain Res 762:240-2. 1997
    ..Postischemic treatment with a free radical scavenger, 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), significantly reduced the increase in DHBA and suppressed delayed neuronal death in the hippocampal CA1 region...
  74. ncbi Thioredoxin, a singlet oxygen quencher and hydroxyl radical scavenger: redox independent functions
    K C Das
    Department of Molecular Biology, University of Texas Health Center at Tyler, 11937 U S Highway 271, Tyler, Texas 75708, USA
    Biochem Biophys Res Commun 277:443-7. 2000
    ..In this report, we present evidence that human thioredoxin is a powerful singlet oxygen quencher and hydroxyl radical scavenger...
  75. ncbi Assembly and analysis of eukaryotic translation initiation complexes
    Andrey V Pisarev
    Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, USA
    Methods Enzymol 430:147-77. 2007
    ..UV) cross-linking to determine the interactions of individual nucleotides in mRNA with components of the initiation complex and for directed hydroxyl radical probing to determine the position of initiation factors on the ribosome.
  76. pmc Temporal variation of hydroxyl radical generation and 8-hydroxy-2'-deoxyguanosine formation by coarse and fine particulate matter
    T Shi
    Institut fur umweltmedizinische Forschung IUF an der Heinrich Heine Universitat Dusseldorf gGmbH, Auf m Hennekamp 50, D 40225 Dusseldorf, Germany
    Occup Environ Med 60:315-21. 2003
    ..particulate matter (PM) sampled over time at one sampling location, and to relate the observed effects to the hydroxyl radical (*OH) generating activities and transition metal content of these samples, and to meteorological parameters.
  77. ncbi Inhibition of vascular smooth muscle cell proliferation by the vitamin E derivative pentamethylhydroxychromane in an in vitro and in vivo study: pivotal role of hydroxyl radical-mediated PLCgamma1 and JAK2 phosphorylation
    Cheng Ying Hsieh
    Graduate Institute of Medical Sciences, Taipei Medical University, Taipei 110, Taiwan
    Free Radic Biol Med 49:881-93. 2010
    ..These events, however, were reversed in the presence of Fe(2+). Moreover, PMC directly inhibited hydroxyl radical formation in both the Fenton reaction and VSMCs according to an electron spin resonance study...
  78. ncbi Generation of hydroxyl radical in isolated pea root cell wall, and the role of cell wall-bound peroxidase, Mn-SOD and phenolics in their production
    Biljana Kukavica
    Institute for Multidisciplinary Research, Belgrade, Serbia
    Plant Cell Physiol 50:304-17. 2009
    The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation...
  79. pmc Active site binding and sequence requirements for inhibition of HIV-1 reverse transcriptase by the RT1 family of single-stranded DNA aptamers
    Jay D Kissel
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Nucleic Acids Res 35:5039-50. 2007
    ..These data suggest a model of ssDNA aptamer-RT interactions and provide an improved molecular understanding of a potent, broad-spectrum ssDNA aptamer...
  80. ncbi Clindamycin phosphate scavenges hydroxyl radical
    Emiko Sato
    New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan
    Int J Dermatol 46:1185-7. 2007
    ..Clindamycin phosphate (CLDMP) is effective against Gram-positive and anaerobic bacteria, and is known to have anti-inflammatory properties...
  81. ncbi ESR study on the structure and hydroxyl radical-scavenging activity relationships of ginsenosides isolated from Panax ginseng C A Meyer
    Ki Sung Kang
    Institute of Natural Medicine, University of Toyama, Toyama, Japan
    Biol Pharm Bull 30:917-21. 2007
    ..The hydroxyl radical (*OH)-scavenging activity test using an electron spin resonance spectrometer (ESR) is suggested to be the ..
  82. ncbi Cupric ion reducing antioxidant capacity assay for antioxidants in human serum and for hydroxyl radical scavengers
    Resat Apak
    Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, Turkey
    Methods Mol Biol 594:215-39. 2010
    ..OH scavengers, the difference being proportional to the scavenging ability of the tested compound. The developed method is less lengthy, more specific, and of a higher yield than the classical TBARS assay...
  83. ncbi Superoxide, hydroxyl radical, and hydrogen peroxide effects on single-diaphragm fiber contractile apparatus
    L A Callahan
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44109, USA
    J Appl Physiol (1985) 90:45-54. 2001
    ..In this study, we evaluated the effect of the superoxide anion radical (O(2)(-).), hydroxyl radical (...
  84. pmc Monitoring singlet oxygen and hydroxyl radical formation with fluorescent probes during photodynamic therapy
    Michael Price
    Cancer Biology Program, and Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA
    Photochem Photobiol 85:1177-81. 2009
    ..1O2 can give rise to additional reactive oxygen species (ROS) such as the hydroxyl radical (*OH)...
  85. pmc rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit
    Byung Sik Shin
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2427, USA
    Mol Cell Biol 29:808-21. 2009
    ..b>Hydroxyl radical mapping experiments reveal that the domain II suppressors interface with the body of the 40S subunit in the ..
  86. ncbi A detailed interpretation of OH radical footprints in a TBP-DNA complex reveals the role of dynamics in the mechanism of sequence-specific binding
    N Pastor
    Facultad de Ciencias, UAEM, Av Universidad 1001, Col Chamilpa, Cuernavaca, Morelos, 62210, Mexico
    J Mol Biol 304:55-68. 2000
    The hydroxyl radical footprint of the TATA-binding protein (TBP) bound to the high-affinity sequence TATAAAAG of the adenovirus 2 major late promoter has been quantitatively compared to a 2 ns molecular dynamics simulation of the complex ..
  87. ncbi Sialic acid is an essential moiety of mucin as a hydroxyl radical scavenger
    Yuki Ogasawara
    Department of Environmental Biology, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Tokyo 204 8588, Japan
    FEBS Lett 581:2473-7. 2007
    ..The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM)...
  88. ncbi Transition metal-induced apoptosis in lymphocytes via hydroxyl radical generation, mitochondria dysfunction, and caspase-3 activation: an in vitro model for neurodegeneration
    Marlene Jimenez Del Rio
    Facultad de Medicina, Departamento de Medicina Interna, Programa de Investigación en Neurociencias, Universidad de Antioquia, Medellin, Colombia
    Arch Med Res 35:185-93. 2004
    ..The objective of the study was to determine the mechanism by which metals induced apoptosis in human peripheral blood lymphocytes (PBL)...
  89. ncbi 3,4-Dihydroxyphenylacetaldehyde and hydrogen peroxide generate a hydroxyl radical: possible role in Parkinson's disease pathogenesis
    S W Li
    Department of Chemistry, Veterans Affairs Medical Center and St. Louis University Medical School, St. Louis, MO 63110, USA
    Brain Res Mol Brain Res 93:1-7. 2001
    ..To determine if DOPAL and other DA metabolites generate the hydroxyl radical in the presence of H(2)O(2), we used HPLC-EC to detect salicylate hydroxylation products...
  90. ncbi Footprinting protein-DNA complexes using the hydroxyl radical
    Swapan S Jain
    Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, USA
    Nat Protoc 3:1092-1100. 2008
    b>Hydroxyl radical footprinting has been widely used for studying the structure of DNA and DNA-protein complexes...
  91. ncbi Novel fluorometric assay for hydroxyl radical prevention capacity using fluorescein as the probe
    Boxin Ou
    Brunswick Laboratories, 6 Thatcher Lane, Wareham, Massachusetts 02571, USA
    J Agric Food Chem 50:2772-7. 2002
    A novel fluorometric method has been developed to evaluate hydroxyl radical prevention capacity using fluorescein (FL) as the probe...
  92. pmc Imidazole metalloporphyrins as photosensitizers for photodynamic therapy: role of molecular charge, central metal and hydroxyl radical production
    Pawel Mroz
    Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA
    Cancer Lett 282:63-76. 2009
    ..Thus, the cationic Pd porphyrin has high potential as a photosensitizer and gives insights into characteristics for improved molecular designs...
  93. ncbi Effect of extracts from pine needle against oxidative DNA damage and apoptosis induced by hydroxyl radical via antioxidant activity
    Jin Boo Jeong
    College of Natural Science, Andong National University, 388, Songcheon Dong, Andong 760749, Republic of Korea
    Food Chem Toxicol 47:2135-41. 2009
    ..the protective effects of water extracts from pine needle (WEPN) against DNA damage and apoptosis induced by hydroxyl radical were investigated in non-cellular and cellular system...
  94. pmc Complexes of mismatched and complementary DNA with minor groove binders. Structures at nucleotide resolution via an improved hydroxyl radical cleavage methodology
    Dobroslawa Bialonska
    Chemistry Department, Wesleyan University, Middletown, CT 06459, USA
    Mutat Res 726:47-53. 2011
    ..Nucleotide resolution structural information can be obtained using an improved hydroxyl radical cleavage protocol...
  95. ncbi Accelerated chemistry in the reaction between the hydroxyl radical and methanol at interstellar temperatures facilitated by tunnelling
    Robin J Shannon
    School of Chemistry, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK
    Nat Chem 5:745-9. 2013
    ..Here we show that, despite the presence of a barrier, the rate coefficient for the reaction between the hydroxyl radical (OH) and methanol--one of the most abundant organic molecules in space--is almost two orders of magnitude ..
  96. ncbi Rejuvenation of degenerative thymus by oral melatonin administration and the antagonistic action of melatonin against hydroxyl radical-induced apoptosis of cultured thymocytes in mice
    Y M Tian
    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China
    J Pineal Res 31:214-21. 2001
    ....
  97. ncbi Growth inhibitory factor prevents neurite extension and the death of cortical neurons caused by high oxygen exposure through hydroxyl radical scavenging
    Yoko Uchida
    Department of Neuropathology, Tokyo Metropolitan Institute of Gerontology, 35 2 Sakaecho, Itabashiku, Tokyo 173 0015, Japan
    J Biol Chem 277:32353-9. 2002
    ..These results indicate that GIF prevents neurite extension of neurons in the early period of differentiation and supports the survival of differentiated neurons by scavenging hydroxyl radicals...
  98. ncbi Effect of the hydroxyl radical scavenger, dimethylthiourea, on peripheral nerve tissue perfusion, conduction velocity and nociception in experimental diabetes
    N E Cameron
    Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Scotland, UK
    Diabetologia 44:1161-9. 2001
    ....
  99. ncbi A new model of tropospheric hydroxyl radical concentrations
    Kiren Bahm
    Department of Physics, Portland State University, SB2 Room 246, P O Box 751, 1719 SW 10th Avenue, Portland, OR 97207 0751, USA
    Chemosphere 54:143-66. 2004
    A chemistry model of the global troposphere is presented which focuses on the hydroxyl radical, OH...
  100. ncbi DNA methylation by dimethyl sulfoxide and methionine sulfoxide triggered by hydroxyl radical and implications for epigenetic modifications
    Kazuaki Kawai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Bioorg Med Chem Lett 20:260-5. 2010
    ..In addition to m(5)dC, the formation of 8-methyldeoxyguanosine (m(8)dG) was also detected in DNA under the same reaction conditions. The m(8)dG level in human DNA may be a useful indicator of DNA methylation by radical mechanisms...
  101. ncbi Antimicrobial peptide pleurocidin synergizes with antibiotics through hydroxyl radical formation and membrane damage, and exerts antibiofilm activity
    Hyemin Choi
    College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea
    Biochim Biophys Acta 1820:1831-8. 2012
    ..However, the synergistic activity and mechanism(s) of pleurocidin in combination with conventional antibiotics, and the antibiofilm effect of the peptide are poorly understood...

Research Grants65

  1. A Hybrid PARP Inhibitor and Redox Catalyst for Lung Transplantation
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..anion that is subsequently converted into powerful toxins, including peroxynitrite, hydrogen peroxide, and hydroxyl radical. These oxidizing and nitrosating species induce DNA single strand breaks that activate poly(ADP-ribose) ..
  2. Mechanism-Based Discovery of Protectants Against Iron-Dependant Oxidative Injury
    JUSTIN M SCHWARTZ; Fiscal Year: 2011
    ..The most reactive and toxic ROS is the hydroxyl radical (OH7) formed by the iron- catalyzed Fenton reaction...
  3. Scavenging of Reactive Oxygen and Nitrogen Species by Cobalamins
    NICOLA ELIZABETH BRASCH; Fiscal Year: 2011
    ..elevated levels of Reactive Oxygen and Nitrogen Species (ROS/RNS), such as nitric oxide, superoxide, the hydroxyl radical, hydrogen peroxide and peroxynitrite...
  4. Hepatocyte Nox proteins in the pathogenesis of hepatocellular carcinoma
    Jinah Choi; Fiscal Year: 2013
    ..Studies suggest that HCV has a direct oncogenic potential and increases the generation of hydroxyl radical and peroxynitrite close to the cell nucleus, inflicting DNA damage that can be mutagenic in the development ..
  5. Androgen Receptor-JunD Complex Inhibitors to Prevent Prostate Cancer Progression
    Hirak S Basu; Fiscal Year: 2012
    ..led to a class of natural cancer causative agents, cellular reactive oxygen species (ROS) such as superoxide, hydroxyl radical, hydrogen peroxide, etc...
  6. IRES-mediated translation initiation on viral mRNAs
    CHRISTOPHER ULRICH TRISTRAM HELLEN; Fiscal Year: 2013
    ..characterize interactions between factors and the IRES using chemical/enzymatic footprinting and directed hydroxyl radical cleavage...
  7. Redox regulation in aging and failing heart
    Junichi Sadoshima; Fiscal Year: 2013
    ..cluster containing enzymes, thereby liberating free iron, which in turn generates the highly reactive hydroxyl radical. O2- produced in cells is rapidly converted to H2O2, while O2- also reacts with NO to form ONOO- , all of ..
  8. Novel mechanisms by which aspirin might protect against atherosclerosis
    Sampath Parthasarathy; Fiscal Year: 2012
    ..We recently observed that aspirin is hydrolyzed to salicylic acid, a potent antioxidant and a hydroxyl radical trapping agent, by human plasma and HDL subfraction...
  9. Reactive oxygen species in Ni(II) carcinogenesis
    Xianglin Shi; Fiscal Year: 2012
    ..oxidase via cdc42 and p47phox to produce superoxide radical, which is then converted to hydrogen peroxide and hydroxyl radical. Specific Aim 2 will test the hypothesis that ROS are required for activation of NFAT and NFkappaB in cells ..
  10. Structure and dynamics of the Tetrahymena telomerase ribonucleoprotein
    Michael D Stone; Fiscal Year: 2013
    ..dimensional organization of conserved RNA and protein domains within the core telomerase RNP using targeted-hydroxyl radical probing, smFRET-based structure measurements, and x-ray crystallography...
  11. Antibiotic pressure and selection of TCA cycle mutants in Staphylococcus epidermi
    Paul D Fey; Fiscal Year: 2010
    ..In addition, bactericidal antibiotics did not induce hydroxyl radical formation in the TCA cycle mutants...
  12. Process for Removal of Microcontaminants from Municipal Water
    JAMES H WHITE; Fiscal Year: 2010
    ..The approach will employ adsorption, electrochemical hydrogen peroxide generation, electrolytic hydroxyl radical generation, and catalytic oxidation unit operations integrated into a compact device...
  13. Role of Neuronal NOS in Neurotoxicity
    Stephen M Black; Fiscal Year: 2010
    ..Our data also indicate that perinatal HI leads to increased generation of the hydroxyl radical and that this is NO-dependent...
  14. RNA IN VIRAL DNA PACKAGING
    Shelley Grimes; Fiscal Year: 2012
    ..the pRNA intermolecular pseudoknot and the essential CCA bulge of the A-helix;3) Solution and site-directed hydroxyl radical probing will be used to map pRNA nucleotides in prohead/pRNA complexes with and without the ATPase gp16;4) ..
  15. Structures and complexes of vertebrate telomere repeat DNAs.
    PHILIP BOLTON; Fiscal Year: 2009
    ..The methods to be validated include circular dichroism and hydroxyl radical cleavage. The validated methods will be used to determine the structures of vertebrate telomere DNAs...
  16. Cancer Prevention/Risk Assessments from DNA Modification Levels
    Nathalie Zeitouni; Fiscal Year: 2010
    ..The assay measures 5 base modifications, at least two of which are clearly related to hydroxyl radical activity...
  17. Tertiary Structure Discovery in the HIV-1 Genome
    Nathan A Siegfried; Fiscal Year: 2012
    ..I will use two approaches to locate tertiary structure: hydroxyl radical probing, and differential SHAPE reactivity...
  18. RNA folding: from global structure to atomic detail
    Lois Pollack; Fiscal Year: 2012
    ..small angle x-ray scattering to report the time-dependent formation of global structures, time-resolved hydroxyl radical footprinting to report the formation of individual contacts within the folding molecule, and time-resolved ..
  19. MECHANISMS OF NEUROPATHIC PAIN
    JIN M CHUNG; Fiscal Year: 2013
    ..but sLTD in GABAn because two different intracellular signaling free radicals are involved, superoxide and hydroxyl radical, respectively...
  20. Lipid peroxidation-induced chemical modifications of insulin signaling proteins
    Krisztian Stadler; Fiscal Year: 2012
    ..The extensive protein radical formation and 4- hydroxynonenal accumulation is mediated by hydroxyl radical production;the lipid radical formation and lipid peroxidation were initiated by the hydroxyl radical derived ..
  21. Mapping The Domain Architecture And Conformations of Soluble Guanylate Cyclase
    ERIC STEVEN UNDERBAKKE; Fiscal Year: 2012
    ..To characterize the relative orientations of the sGC domains, hydroxyl radical footprinting will be employed...
  22. Mechanistic Studies of Nucleic Acid Damage and Their Applications.
    Marc M Greenberg; Fiscal Year: 2013
    ..radicals in RNA oxidation and determine whether this can be used to learn more about its structure in hydroxyl radical (OH7) cleavage experiments (Aim 3)...
  23. OXIDANT MECHANISMS IN MEMBRANOUS NEPHROPATHY
    Sudhir Shah; Fiscal Year: 1993
    ..We have recently shown marked reduction in proteinuria in PHN with hydroxyl radical scavengers and an iron chelator...
  24. De Novo Design of a Novel RNA Fold
    ELISABETH LYN NARAYANAN; Fiscal Year: 2013
    ..b>Hydroxyl radical foot-printing will then be used to probe whether designed sequences adopt de novo tertiary structures ..
  25. CONTROL OF CELL FUNCTION VIA SELECTIVE MRNA TRANSLATION
    Lee Gehrke; Fiscal Year: 2002
    ..in the viral coat proteins, 2) the identification, through use of chemical modification interference and hydroxyl radical footprint analysis, of the coat protein binding site on viral RNA, and 3) the characterization of 3' ..
  26. Structural and temporal analyses of the folding landscape of the B12 riboswitch
    JAMES EDWARD JOHNSON; Fiscal Year: 2012
    ..In Aim 2, time-resolved hydroxyl radical footprinting will investigate the rate of aptamer folding in the presence of magnesium and if AdoCbl aids ..
  27. Radiolytic Footprinting Methods for Structural Mass Spectrometry
    Mark R Chance; Fiscal Year: 2013
    ..provided by applicant): We have developed innovative methods of structural mass spectrometry based on hydroxyl radical modification of proteins...
  28. Time-resolved hydroxyl radical footprinting of RNA
    Sarah A Woodson; Fiscal Year: 2013
    ....
  29. LEUKOCYTE, ENZYME AND DRUG CHEMILUMINESCENCE STUDIES
    JEFFREY KANOFSKY; Fiscal Year: 1990
    ..complex oxidative mechanisms in which transient, highly reactive intermediates such as superoxide anion, hydroxyl radical and singlet molecular oxygen play a major role...
  30. Conformational Conversions of Prion Protein
    Witold K Surewicz; Fiscal Year: 2013
    ..study will employ a number of complementary biophysical methods including site-directed spin labeling, solid-state NMR spectroscopy, hydrogen/deuterium exchange, and hydroxyl radical-mediated footprinting coupled with mass spectrometry.
  31. Improved Hydroxyl Radical Footprinting for Modeling Protein Structure
    Joshua S Sharp; Fiscal Year: 2013
    ..We propose to characterize the various broadly neutralizing antibody epitopes using hydroxyl radical protein footprinting of the gp120-antibody complexes, a technique that labels a broad variety of amino acid ..
  32. PATHOGENESIS OF MINERAL DUST TOXICITY
    Agnes Kane; Fiscal Year: 1991
    ..and crocidolite asbestos fibers generates reactive oxygen metabolites, including the highly reactive hydroxyl radical. Asbestos toxicity in vitro or in vivo is prevented by free radical scavenging enzymes or the iron chelator, ..
  33. MODELS FOR EXPLORING FREE RADICAL DAMAGE
    Julie Andersen; Fiscal Year: 1999
    ..is disturbed and free-radicals are allowed to accumulate, this can lead to iron- catalyzed production of hydroxyl radical and subsequent cell damage...
  34. OXIDATION OF MACROMOLECULES IN SPINAL CORD INJURY
    Danxia Liu; Fiscal Year: 2001
    ..The time course of hydrogen peroxide and hydroxyl radical in the extracellular space will be measured by microdialysis sampling; 8-OHdG, PCC and NTyr are measured, ..
  35. Structural characterization of RNA recognition during Staufen-mediated decay
    MICHAEL LEONARD GLEGHORN; Fiscal Year: 2011
    ..shape, and 2) determine the regions of interaction between Staufen and ARF1 SBS RNA by directed hydroxyl radical footprinting and RNase footprlnting assays as well as obtaining an atomic description of the Staufen-ARF1 SBS ..
  36. GENE MANIPULATION & H202 DEGRADATION IN THE OCULAR LENS
    Abraham Spector; Fiscal Year: 2001
    ..of cataract patients and experiments suggest that even where other potent oxidants such as superoxide and hydroxyl radical are present, removal of H2O2 eliminates the stress induced formation of cataract...
  37. HYDROXYL RADICAL BIOLOGY BY CEREBRAL ISCHEMIA
    Philip Liu; Fiscal Year: 1999
    ..b>Hydroxyl radical, a known mutagen, induces DNA repair synthesis and the expression of repair enzyme DNA polymerase-beta in ..
  38. MITOCHONDRIA INCREASE OXIDATIVE INJURY IN AGING HEART
    EDWARD LESNEFSKY JR; Fiscal Year: 1999
    ..that greater mitochondrial injury increases mitochondrial production of the very reactive and damaging hydroxyl radical (OH) during reperfusion...
  39. OXYGEN AND SPONTANEOUS MUTAGENESIS IN ESCHERICHIA COLI
    Robert Fowler; Fiscal Year: 1991
    ..These toxic byproducts of aerobic respiration, which include superoxide radical, hydrogen peroxide and hydroxyl radical, are thought to be possible causative agents in mutagenesis, carcinogenesis and aging...
  40. NOT STATED
    Marius Brouwer; Fiscal Year: 1992
    ..Through its oxidation of GSH and its catalysis of superoxide and hydroxyl radical formation, copper may play a crucial role in the generation of oxidative stress...
  41. Trimetallic Nitride Templated Endohedral Metallofullerenes as Dual Imaging and Mu
    MARISSA RYLANDER; Fiscal Year: 2009
    ..and hydroxyl radical (.OH) following laser excitation of cells...
  42. FOOTPRINTING WITH IRON (II)-GENERATED HYDROXYL RADICAL
    THOMAS TULLIUS; Fiscal Year: 2003
    The reaction of iron(II) EDTA with hydrogen peroxide produces the hydroxyl radical, which has proven to be a powerful and widely- used chemical probe of the structure of protein-DNA and protein- RNA complexes...
  43. INTESTINAL ISCHEMIA--ROLE OF OXYGEN RADICALS
    D Granger; Fiscal Year: 1992
    ..Upon reperfusion (reoxygenation) a burst of superoxide, hydrogen peroxide, and hydroxyl radical results from the oxidation of hypoxanthine by xanthine oxidase...
  44. CEREBROVASCULAR INJURY FROM OXYGEN RADICALS
    Bruce Freeman; Fiscal Year: 1991
    ..Excessive production of reactive oxygen species including superoxide (O2-), hydrogen peroxide (H2O2) and hydroxyl radical (OH-) occurs secondary to diverse phenomena including trauma, acute inflammation sepsis, tissue ischemia-..
  45. MONOCYTE/MACROPHAGE FREE RADICAL BIOLOGY ASSESSED BY EPR
    Bradley Britigan; Fiscal Year: 1993
    ..In vitro these two oxidant species react in the presence of a transition metal catalyst (e.g. iron) to form hydroxyl radical (OH)...
  46. FREE RADICAL AND PEROXIDATIVE DAMAGE IN SPINE
    Danxia Liu; Fiscal Year: 2000
    ..Although a variety of free radical species may be involved in peroxidative damage hydroxyl radical is the most destructive of these...
  47. ROLE OF NITRIC OXIDE IN SUPEROXIDE-MEDIATED PATHOLOGY
    JOSEPH BECKMAN; Fiscal Year: 1993
    ..4 and 37oC before undergoing proton-catalyzed decomposition to form potent oxidants, which are most likely hydroxyl radical and nitrogen dioxide: (pKa=6...
  48. BIOMETALS, OXIDATIVE STRESS, AND AB AMYLOIDOSIS
    Xudong Huang; Fiscal Year: 2005
    ..Cu(I) and Fe(II), and the consequent generation of reactive oxygen species (ROS)-hydrogen peroxide (H2O2) and hydroxyl radical (OH). These abnormal interactions may lead to the brain oxidation damage that is observed in AD...
  49. DYNAMICS OF MACROMOLECULAR INTERACTIONS
    MARK CHANCE; Fiscal Year: 2001
    ..In the R21 phase of this proposal, we will develop a quantitative hydroxyl radical "footprinting" technique using synchrotron radiation to probe the structure of proteins...
  50. DYNAMICS OF MACROMOLECULAR INTERACTIONS
    MARK CHANCE; Fiscal Year: 2003
    ..In the R21 phase of this proposal, we will develop a quantitative hydroxyl radical "footprinting" technique using synchrotron radiation to probe the structure of proteins...
  51. OXY-RADICALS IN GENTAMICIN AND OTHER TOXIC NEPHROPATHIES
    Patrick Walker; Fiscal Year: 1992
    ..to examine the effect of gentamicin on the generation of hydroxyl radical by renal cortical mitochondria. B...
  52. The Role of Quinols in Estrogen Neuroprotection
    Laszlo Prokai; Fiscal Year: 2006
    ..ArO*) stability, electron density of ArO* at the C-1i0 position and, thus, its reactivity towards hydroxyl radical (-0H). The Fenton-reaction, which leads to 0H formation, will be employed as a chemical model...
  53. DOPAMINE NERVES SUPRESS HYDROXYL RADICAL FORMATION IN NE
    RICHARD KOSTRZEWA; Fiscal Year: 1999
    ..to reactive o-quinones and is most involved in generation of other reactive oxygen species (ROS), of which hydroxyl radical (OH) is the most reactive...
  54. Mechanisms of I/R Injury to Hepatocytes
    John J Lemasters; Fiscal Year: 2010
    ..lysosomes (first hit) and mitochondrial superoxide/hydrogen peroxide generation (second hit) acts to initiate hydroxyl radical formation and peroxidation chain reactions to promote MPT onset after I/R;3) the same mechanisms causing I/R ..
  55. STRATEGIES TO LIMIT DEFIBRILLATION AND REPERFUSION INJUR
    Richard Kerber; Fiscal Year: 2002
    ..form peroxynitrite, which is toxic in itself and also forms further strongly oxidizing species resembling the hydroxyl radical. By limiting nitric oxide availability, nitric oxide synthase inhibitors may limit this process and ..
  56. REGULATION AND FUNCTION OF HEME OXYGENASE-1
    SHARON CAMHI; Fiscal Year: 1999
    ..which sequesters intracellular free iron, making the iron unavailable to participate in the Fenton reaction, hydroxyl radical formation, and subsequent cellular damage...
  57. DNA MISMATCH REPAIR IN EUKARYOTES
    Satya Prakash; Fiscal Year: 2003
    ..Mismatch binding by the Msh2-Msh3 and Msh2-Msh6 complexes will be examined by Dnase I and hydroxyl radical footprinting, and the effect of Mlh1-Pms1 on mismatch binding by Msh complexes determined...
  58. DEFERIPRONE THERAPY FOR SICKLE CELL DISEASE
    Nancy Olivieri; Fiscal Year: 2001
    ..Removal of iron from the red cell membrane would be expected to reduce the generation of hydroxyl radical, and represents a novel approach to the therapy of sickle cell disease...
  59. OXYGEN AND OXY-RADICALS IN PHAGOCYTIC KILLING
    Michael Thomas; Fiscal Year: 1990
    ..initiated by a variety of oxy-radicals; 2) dedecyl 4-phenylbutyrate will be used to probe for the hydroxyl radical; 3) ethyl 6-(3'-5'-dimethoxyphenoxyl) hexanoate will be used to probe for and to quantify HOCl formation; and ..
  60. ULCERATIVE COLITIS--ROLE OF REACTIVE OXYGEN METABOLITES
    Matthew Grisham; Fiscal Year: 1992
    ..Although the sources of superoxide radical, hydrogen peroxide and hydroxyl radical in the colon have not been identified, certain mucosal oxidases as well as resident and inflammatory ..
  61. REGULATION OF MUCIN GENE EXPRESSION BY ELASTASE
    Judith Voynow; Fiscal Year: 2003
    ..elastase treatment of airway epithelial cells triggers generation of reactive oxygen species; and 3) hydroxyl radical and hydrogen peroxide scavengers inhibit neutrophil elastase-induced increases in MUC5AC and MUC4 mRNA levels...
  62. XANTHINE OXIDASE-ENDOTHELIAL GLYCOSAMINOGLYCAN BINDING
    Bruce Freeman; Fiscal Year: 1992
    ..O2) and hydrogen peroxide (H2O2), which can react further to create secondary toxic species such as hydroxyl radical (OH) or lipid hydroperoxides...
  63. The nucleic acid specificity and endogenous substrate of Rei1
    KENNETH PARNELL; Fiscal Year: 2007
    ..unreadable] [unreadable] To identify the endogenous substrate of Reilp, I will utilize hydroxyl radical probing to determine ReMp's large subunit RNA "footprint"...
  64. Hydroxyl radical mapping of protein interfaces
    Vernon Anderson; Fiscal Year: 2005
    ..This proposal describes development of a complementary method to amide H/D-Ex, specifically hydroxyl radical-mediated isotope exchange into aliphatic carbons of amino acid side chains, a technique referred to as alkyl ..
  65. STRESS RESPONSE PROTECT AGAINST REPERFUSION INJURY
    Ching Yuan Su; Fiscal Year: 1999
    ..Adapted from Applicant' Abstract) Reactive oxygen species, such as superoxide, hydrogen peroxide, and hydroxyl radical, are formed excessively during the initial phase of reoxygenation of the ischemic heart...