pilocarpine

Summary

Summary: A slowly hydrolyzed muscarinic agonist with no nicotinic effects. Pilocarpine is used as a miotic and in the treatment of glaucoma.

Top Publications

  1. ncbi Expression profile of microRNAs in rat hippocampus following lithium-pilocarpine-induced status epilepticus
    Kai Hu
    Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China
    Neurosci Lett 488:252-7. 2011
  2. pmc The pilocarpine model of temporal lobe epilepsy
    Giulia Curia
    Montreal Neurological Institute and Departments of Neurology and Neurosurgery and Physiology, McGill University, Montreal, QC, Canada
    J Neurosci Methods 172:143-57. 2008
  3. pmc Changes in microRNA expression in the whole hippocampus and hippocampal synaptoneurosome fraction following pilocarpine induced status epilepticus
    Rashmi M Risbud
    Division of Pediatric Neurology, Department of Pediatrics, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 8:e53464. 2013
  4. ncbi The COX-2 inhibitor parecoxib is neuroprotective but not antiepileptogenic in the pilocarpine model of temporal lobe epilepsy
    Nadine Polascheck
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, Germany
    Exp Neurol 224:219-33. 2010
  5. ncbi Mesial temporal lobe epilepsy: pathogenesis, induced rodent models and lesions
    Alok K Sharma
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
    Toxicol Pathol 35:984-99. 2007
  6. ncbi Behavioral and cognitive alterations, spontaneous seizures, and neuropathology developing after a pilocarpine-induced status epilepticus in C57BL/6 mice
    Christine J Müller
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, Bunteweg 17, D 30559 Hannover, Germany
    Exp Neurol 219:284-97. 2009
  7. ncbi Pilocarpine vs. lithium-pilocarpine for induction of status epilepticus in mice: development of spontaneous seizures, behavioral alterations and neuronal damage
    Christine J Müller
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine, Bunteweg 17, D 30559 Hannover, Germany
    Eur J Pharmacol 619:15-24. 2009
  8. ncbi A gelatin-g-poly(N-isopropylacrylamide) biodegradable in situ gelling delivery system for the intracameral administration of pilocarpine
    Jui Yang Lai
    Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan 33302, Republic of China
    Biomaterials 33:2372-87. 2012
  9. ncbi Innate and adaptive immunity during epileptogenesis and spontaneous seizures: evidence from experimental models and human temporal lobe epilepsy
    Teresa Ravizza
    Department of Neuroscience, Laboratory of Experimental Neurology, Mario Negri Institute for Pharmacological Research, Milano, Italy
    Neurobiol Dis 29:142-60. 2008
  10. pmc Enhanced tonic GABA current in normotopic and hilar ectopic dentate granule cells after pilocarpine-induced status epilepticus
    Ren Zhi Zhan
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Neurophysiol 102:670-81. 2009

Research Grants

  1. Prostaglandin signaling following seizures
    Jianxiong Jiang; Fiscal Year: 2013
  2. Mechanism and Treatment of nerve agent-induced seizures
    Jaideep Kapur; Fiscal Year: 2010
  3. Subunit dependent properties of kainate receptors
    David D Mott; Fiscal Year: 2013
  4. Mechanisms of comorbidity between epilepsy and depression
    Andrey M Mazarati; Fiscal Year: 2013
  5. Neurobiological basis of Depression as a Comorbidity in Epilepsy
    Andrey M Mazarati; Fiscal Year: 2010
  6. QUANTITATIVE GENETIC STUDY OF SEIZURES
    Thomas N Ferraro; Fiscal Year: 2012
  7. Rational polytherapy in the treatment of cholinergic seizures
    Claude G Wasterlain; Fiscal Year: 2013
  8. mTOR dysregulation in Epilepsy
    Angela N Carter; Fiscal Year: 2013
  9. Stem Cell Transplantation for Epilepsy
    JANICE RAE NAEGELE; Fiscal Year: 2013
  10. Molecular Determinants of GABAA Receptor Gene Regulation
    SHELLEY JUNE RUSSEK; Fiscal Year: 2010

Detail Information

Publications314 found, 100 shown here

  1. ncbi Expression profile of microRNAs in rat hippocampus following lithium-pilocarpine-induced status epilepticus
    Kai Hu
    Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China
    Neurosci Lett 488:252-7. 2011
    ....
  2. pmc The pilocarpine model of temporal lobe epilepsy
    Giulia Curia
    Montreal Neurological Institute and Departments of Neurology and Neurosurgery and Physiology, McGill University, Montreal, QC, Canada
    J Neurosci Methods 172:143-57. 2008
    ..lobe epilepsy (TLE) largely rests on the use of models of status epilepticus (SE), as in the case of the pilocarpine model...
  3. pmc Changes in microRNA expression in the whole hippocampus and hippocampal synaptoneurosome fraction following pilocarpine induced status epilepticus
    Rashmi M Risbud
    Division of Pediatric Neurology, Department of Pediatrics, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 8:e53464. 2013
    ..microRNA arrays were performed on rat hippocampus 4 hours, 48 hours and 3 weeks following an episode of pilocarpine induced status epilepticus. Eighty microRNAs increased at one or more of the time points...
  4. ncbi The COX-2 inhibitor parecoxib is neuroprotective but not antiepileptogenic in the pilocarpine model of temporal lobe epilepsy
    Nadine Polascheck
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, Germany
    Exp Neurol 224:219-33. 2010
    ..pro-drug of the highly potent and selective COX-2 inhibitor valdecoxib, alters the long-term consequences of a pilocarpine-induced SE in rats. Parecoxib was administered twice daily at 10 mg/kg for 18 days following SE...
  5. ncbi Mesial temporal lobe epilepsy: pathogenesis, induced rodent models and lesions
    Alok K Sharma
    Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
    Toxicol Pathol 35:984-99. 2007
    ..Among post-status epilepticus models, induction of systemic kainic acid or pilocarpine-induced epilepsy is less labor-intensive than electrical-stimulation models and these models mirror the ..
  6. ncbi Behavioral and cognitive alterations, spontaneous seizures, and neuropathology developing after a pilocarpine-induced status epilepticus in C57BL/6 mice
    Christine J Müller
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, Bunteweg 17, D 30559 Hannover, Germany
    Exp Neurol 219:284-97. 2009
    ..Animal models of epilepsy, such as the pilocarpine model of acquired temporal lobe epilepsy (TLE), are useful to study the relationship between epilepsy and ..
  7. ncbi Pilocarpine vs. lithium-pilocarpine for induction of status epilepticus in mice: development of spontaneous seizures, behavioral alterations and neuronal damage
    Christine J Müller
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine, Bunteweg 17, D 30559 Hannover, Germany
    Eur J Pharmacol 619:15-24. 2009
    In recent years, the pilocarpine model of temporal lobe epilepsy has become the most popular and widely used rodent model of this common and difficult-to-treat type of epilepsy...
  8. ncbi A gelatin-g-poly(N-isopropylacrylamide) biodegradable in situ gelling delivery system for the intracameral administration of pilocarpine
    Jui Yang Lai
    Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan 33302, Republic of China
    Biomaterials 33:2372-87. 2012
    ..95%) were achieved for GN vehicles, which was attributed to initial fast temperature triggered capture of pilocarpine and subsequent progressive degradation of gelatin network...
  9. ncbi Innate and adaptive immunity during epileptogenesis and spontaneous seizures: evidence from experimental models and human temporal lobe epilepsy
    Teresa Ravizza
    Department of Neuroscience, Laboratory of Experimental Neurology, Mario Negri Institute for Pharmacological Research, Milano, Italy
    Neurobiol Dis 29:142-60. 2008
    ..These data show that specific inflammatory pathways are chronically activated during epileptogenesis and they persist in chronic epileptic tissue, suggesting they may contribute to the etiopathogenesis of TLE...
  10. pmc Enhanced tonic GABA current in normotopic and hilar ectopic dentate granule cells after pilocarpine-induced status epilepticus
    Ren Zhi Zhan
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Neurophysiol 102:670-81. 2009
    ..from control rats and from both normotopic and hilar ectopic granule cells in slices from rats subjected to pilocarpine-induced status epilepticus...
  11. ncbi Behavioral alterations in the pilocarpine model of temporal lobe epilepsy in mice
    Ina Gröticke
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, and Center for Systems Neuroscience, Hannover, Germany
    Exp Neurol 207:329-49. 2007
    ..Animal models of epilepsy, such as the pilocarpine model of acquired epilepsy, are useful to study the relationship between epilepsy and behavioral dysfunctions...
  12. ncbi Axon sprouting in a model of temporal lobe epilepsy creates a predominantly excitatory feedback circuit
    Paul S Buckmaster
    Department of Comparative Medicine, Stanford University School of Medicine, Stanford, California 94305 5330, USA
    J Neurosci 22:6650-8. 2002
    ..sprouted mossy fibers at the EM level, and identified postsynaptic targets with GABA immunocytochemistry in the pilocarpine model of temporal lobe epilepsy. Granule cells projected an average of 1.0 and 1...
  13. ncbi Pilocarpine-induced status epilepticus results in mossy fiber sprouting and spontaneous seizures in C57BL/6 and CD-1 mice
    Heather Shibley
    Department of Cell and Molecular Biology, Division of Neurobiology, Tulane University, 6400 Freret Street, New Orleans, LA 70118, USA
    Epilepsy Res 49:109-20. 2002
    ..and correlative axon sprouting in the dentate gyrus of CD-1 and C57BL/6 mice after systemic injection of pilocarpine. Pilocarpine induced seizures and status epilepticus (SE) after systemic injection in both strains, although SE ..
  14. ncbi The pilocarpine model of epilepsy: what have we learned?
    Fulvio A Scorza
    Disciplina de Neurologia Experimental, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Botucatu, 862, Edifício José Leal Prado, 04023 900 São Paulo, SP, Brasil
    An Acad Bras Cienc 81:345-65. 2009
    The systemic administration of a potent muscarinic agonist pilocarpine in rats promotes sequential behavioral and electrographic changes that can be divided into 3 distinct periods: (a) an acute period that built up progressively into a ..
  15. ncbi Deep brain stimulation of the anterior nucleus of the thalamus: effects of electrical stimulation on pilocarpine-induced seizures and status epilepticus
    Clement Hamani
    Division of Neurosurgery, Toronto Western Hospital and University Health Network, University of Toronto, Toronto, Ontario, Canada
    Epilepsy Res 78:117-23. 2008
    ..As the optimal stimulation settings remain elusive, we studied the effects of different stimulation parameters against pilocarpine induced seizures and status epilepticus (SE).
  16. ncbi Differences in sensitivity to the convulsant pilocarpine in substrains and sublines of C57BL/6 mice
    C J Muller
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine, D 30559 Hannover, Germany
    Genes Brain Behav 8:481-92. 2009
    In rodents, the cholinomimetic convulsant pilocarpine is widely used to induce status epilepticus (SE), followed by hippocampal damage and spontaneous recurrent seizures, resembling temporal lobe epilepsy...
  17. ncbi Temporal patterns of the cerebral inflammatory response in the rat lithium-pilocarpine model of temporal lobe epilepsy
    Brigitte Voutsinos-Porche
    INSERM U405, Faculty of Medicine, Strasbourg, France
    Neurobiol Dis 17:385-402. 2004
    ..NF-kappaB), and Cyclooxygenase-2 (COX-2) expression together with neurodegeneration in the rat lithium-pilocarpine model...
  18. ncbi Impaired activation of CA3 pyramidal neurons in the epileptic hippocampus
    Giuseppe Biagini
    Dipartimento di Scienze Biomediche, Universita di Modena e Reggio Emilia, 41100 Modena, Italy
    Neuromolecular Med 7:325-42. 2005
    We employed in vitro and ex vivo imaging tools to characterize the function of limbic neuron networks in pilocarpine-treated and age-matched, nonepileptic control (NEC) rats...
  19. ncbi Pilocarpine-induced seizure-like activity with increased BNDF and neuropeptide Y expression in organotypic hippocampal slice cultures
    Frantz Rom Poulsen
    Department of Anatomy and Neurobiology, University of Southern Denmark Odense, Winsloewparken 21, DK 5000 C, Odense, Denmark
    Brain Res 950:103-18. 2002
    Organotypic hippocampal slice cultures were treated with the muscarinic agonist pilocarpine to study induced seizure-like activity and changes in neurotrophin and neuropeptide expression...
  20. pmc Mossy fibers are the primary source of afferent input to ectopic granule cells that are born after pilocarpine-induced seizures
    Joseph P Pierce
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, NY 10021, USA
    Exp Neurol 196:316-31. 2005
    ..EGC dendrites were examined in tissue from epileptic rats that had initially been treated with pilocarpine to induce status epilepticus and subsequently had spontaneous seizures...
  21. pmc Inhibition of the mammalian target of rapamycin signaling pathway suppresses dentate granule cell axon sprouting in a rodent model of temporal lobe epilepsy
    Paul S Buckmaster
    Department of Comparative Medicine, Stanford University, Stanford, California 94305, USA
    J Neurosci 29:8259-69. 2009
    ..infused into the dorsal hippocampus for prolonged periods beginning within hours after rats sustained pilocarpine-induced status epilepticus...
  22. pmc Microglial ablation and lipopolysaccharide preconditioning affects pilocarpine-induced seizures in mice
    Martine M Mirrione
    Department of Pharmacological Sciences, Molecular and Cellular Pharmacology, Stony Brook University, Stony Brook, NY 11794 8651, USA
    Neurobiol Dis 39:85-97. 2010
    ..To explore this, we examined the effects of pilocarpine-induced seizures in transgenic mice where microglia/macrophages were conditionally ablated...
  23. pmc Stereological analysis of GluR2-immunoreactive hilar neurons in the pilocarpine model of temporal lobe epilepsy: correlation of cell loss with mossy fiber sprouting
    Yiqun Jiao
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Box 3813, Durham, North Carolina 27710, USA
    Exp Neurol 205:569-82. 2007
    ..Prolonged pilocarpine-induced status epilepticus killed 95% of these cells...
  24. pmc Acute and chronic responses to the convulsant pilocarpine in DBA/2J and A/J mice
    M R Winawer
    Department of Neurology and G H Sergievsky Center, Columbia University, New York, NY 10032, USA
    Neuroscience 149:465-75. 2007
    ..These strains were compared using the chemoconvulsant pilocarpine, because pilocarpine induces status epilepticus, a state of severe, prolonged seizures...
  25. pmc Reduction in delayed mortality and subtle improvement in retrograde memory performance in pilocarpine-treated mice with conditional neuronal deletion of cyclooxygenase-2 gene
    Jamie R Levin
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia, USA
    Epilepsia 53:1411-20. 2012
    b>Pilocarpine induces prolonged status epilepticus (SE) in rodents that results in neurodegeneration and cognitive deficits, both commonly observed to be associated with human temporal lobe epilepsy...
  26. ncbi Increased perivascular laminin predicts damage to astrocytes in CA3 and piriform cortex following chemoconvulsive treatments
    F Gualtieri
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy
    Neuroscience 218:278-94. 2012
    Status epilepticus (SE) induced by pilocarpine or kainate is associated with yet not systemically investigated astrocytic and vascular injuries...
  27. ncbi Magnetic resonance imaging of changes elicited by status epilepticus in the rat brain: diffusion-weighted and T2-weighted images, regional blood volume maps, and direct correlation with tissue and cell damage
    P F Fabene
    Section of Anatomy and Histology, Department of Morphological and Biomedical Sciences, Faculty of Medicine, University of Verona, Strada Le Grazie 8, 37134 Verona, Italy
    Neuroimage 18:375-89. 2003
    ..brain was investigated with structural and functional magnetic resonance imaging (MRI) 12 h after the arrest of pilocarpine-induced status epilepticus lasting 4 h...
  28. ncbi Proepileptic influence of a focal vascular lesion affecting entorhinal cortex-CA3 connections after status epilepticus
    Giuseppe Biagini
    Dipartimento di Scienze Biomediche, Universita di Modena e Reggio Emilia, Modena, Italy
    J Neuropathol Exp Neurol 67:687-701. 2008
    ..Here, we describe a lesion that destroys the perforant path in CA3 after status epilepticus (SE) induced by pilocarpine injection in 8-week-old rats...
  29. ncbi Generation and characterization of pilocarpine-sensitive C57BL/6 mice as a model of temporal lobe epilepsy
    Marion Bankstahl
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, Germany
    Behav Brain Res 230:182-91. 2012
    ..strain, but its use in epilepsy research is compromised by low sensitivity to various convulsants, including pilocarpine. We recently identified a subline of B6NCrl mice in a barrier (#8) of a German vendor (Charles River) that was ..
  30. ncbi Network dynamics during development of pharmacologically induced epileptic seizures in rats in vivo
    Adi Cymerblit-Sabba
    Department of Physiology, Bruce Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel
    J Neurosci 30:1619-30. 2010
    ..The preictal state preceding pilocarpine-, kainate-, and picrotoxin-induced seizures was characterized by biphasic network dynamics composed of an early ..
  31. ncbi Beneficial effects of desacyl-ghrelin, hexarelin and EP-80317 in models of status epilepticus
    Giuseppe Biagini
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy
    Eur J Pharmacol 670:130-6. 2011
    ..growth hormone secretagogue receptor 1a (GHSR(1a)) ligands in rats exposed to status epilepticus induced by pilocarpine or kainate. Firstly, in three independent experiments, before receiving pilocarpine (380 mg/kg, i.p...
  32. pmc Morpho-physiologic characteristics of dorsal subicular network in mice after pilocarpine-induced status epilepticus
    De Fu He
    Epilepsy Research Lab, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, Singapore
    Brain Pathol 20:80-95. 2010
    ..neurons, particularly calbindin, parvalbumin (PV) and immunopositive interneurons, was found at 2 months after pilocarpine-induced status epilepticus (SE)...
  33. pmc M(3) muscarinic acetylcholine receptor plays a critical role in parasympathetic control of salivation in mice
    Takeshi Nakamura
    Calcium Oscillation Project, Japan Science and Technology Agency, Tokyo, Japan
    J Physiol 558:561-75. 2004
    ..In vivo administration of a high dose of pilocarpine (10 mg kg(-1), s.c...
  34. pmc Degeneration and proliferation of astrocytes in the mouse dentate gyrus after pilocarpine-induced status epilepticus
    Karin Borges
    Department of Pharmacology, Emory University, Atlanta, GA 30322, USA
    Exp Neurol 201:416-27. 2006
    ..Here, we report transient region-specific loss of astrocytes in mice early after pilocarpine-induced status epilepticus (SE)...
  35. pmc P2X7 receptor regulates leukocyte infiltrations in rat frontoparietal cortex following status epilepticus
    Ji Eun Kim
    Department of Anatomy and Neurobiology, Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon 200 702, South Korea
    J Neuroinflammation 7:65. 2010
    ..In the present study, we investigated the roles of P2X7 receptor in recruitment and infiltration of neutrophil during epileptogenesis in rat epilepsy models...
  36. ncbi Investigation of oxidative stress involvement in hippocampus in epilepsy model induced by pilocarpine
    R M Freitas
    Laboratory of Physiology and Pharmacology, Federal University of Piaui, Piaui, Brazil
    Neurosci Lett 462:225-9. 2009
    ..Using the epilepsy model obtained by systemic administration of pilocarpine in rats, we investigated the lipid peroxidation, nitrite content, superoxide dismutase (SOD) and catalase ..
  37. ncbi A muscarinic cholinergic mechanism underlies activation of the central pattern generator for locust flight
    Edgar Buhl
    University of Leipzig, Institute of Biology II, Talstr 33, 04103 Leipzig, Germany
    J Exp Biol 211:2346-57. 2008
    ..In deafferented locusts, fictive flight was initiated by bath application of the muscarinic agonist pilocarpine, the acetylcholine analogue carbachol, and the acetylcholinesterase blocker eserine, but not by nicotine...
  38. ncbi IMPA1 is essential for embryonic development and lithium-like pilocarpine sensitivity
    Kim Cryns
    Research and Early Development Europe, Johnson and Johnson Pharmaceutical Research and Development, Beerse, Belgium
    Neuropsychopharmacology 33:674-84. 2008
    ..activity in both the open-field test and the forced-swim test as well as a strongly increased sensitivity to pilocarpine-induced seizures, the latter supporting the idea that IMPA1 represents a physiologically relevant target for ..
  39. ncbi Cyclooxygenase-2 inhibitor, celecoxib, inhibits the altered hippocampal neurogenesis with attenuation of spontaneous recurrent seizures following pilocarpine-induced status epilepticus
    Keun Hwa Jung
    Neural Stem Cell Laboratory in Clinical Research Institute, Stem Cell Research Center, Department of Neurology, Seoul National University Hospital, Program in Neuroscience, Neuroscience Research Institute of SNUMRC, Seoul, South Korea
    Neurobiol Dis 23:237-46. 2006
    ..Celecoxib attenuated the likelihood of developing spontaneous recurrent seizures after pilocarpine-induced prolonged seizure...
  40. pmc Neuronal activity rapidly induces transcription of the CREB-regulated microRNA-132, in vivo
    Aaron S Nudelman
    Department of Pharmacology, University of Washington, Seattle, Washington
    Hippocampus 20:492-8. 2010
    ..b>Pilocarpine-induced seizures led to a robust, rapid, and transient increase in the primary transcript of miR-132 (pri-miR-..
  41. ncbi Aberrant seizure-induced neurogenesis in experimental temporal lobe epilepsy
    Jack M Parent
    Department of Neurology, University of Michigan Medical Center, 4412 Kresge III, 200 Zina Pitcher Place, Ann Arbor, MI 48109 0585, USA
    Ann Neurol 59:81-91. 2006
    ..and immunohistochemistry were used to identify proliferating progenitors and mature DGCs in the adult rat pilocarpine temporal lobe epilepsy model. We also examined dentate gyri from epileptic human hippocampal surgical specimens...
  42. ncbi Subiculum network excitability is increased in a rodent model of temporal lobe epilepsy
    Philip de Guzman
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
    Hippocampus 16:843-60. 2006
    ..to study the subiculum--a limbic structure that gates the information flow from and to the hippocampus--in pilocarpine-treated epileptic rats. Comparative data were obtained from age-matched nonepileptic controls (NEC)...
  43. ncbi Pilocarpine-induced seizures cause selective time-dependent changes to adult-generated hippocampal dentate granule cells
    Cynthia Walter
    Department of Anesthesia, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Neurosci 27:7541-52. 2007
    ..label granule cells born 8 weeks before (mature), 1 week before (immature), or 3 weeks after (newborn) pilocarpine-epileptogenesis. Neuronal morphology was examined 4 and 8 weeks after pilocarpine treatment...
  44. ncbi Disease-modifying effects of phenobarbital and the NKCC1 inhibitor bumetanide in the pilocarpine model of temporal lobe epilepsy
    Claudia Brandt
    Department of Pharmacology, Toxicology, and Pharmacy, University of Veterinary Medicine Hannover, D 30559 Hannover, Germany
    J Neurosci 30:8602-12. 2010
    ..study, it was evaluated whether prolonged administration of the selective NKCC1 inhibitor, bumetanide, after a pilocarpine-induced SE modifies the development of epilepsy in adult female rats...
  45. ncbi Astroglial loss and edema formation in the rat piriform cortex and hippocampus following pilocarpine-induced status epilepticus
    Ji Eun Kim
    Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon, Kangwon Do 200 702, South Korea
    J Comp Neurol 518:4612-28. 2010
    ..study we analyzed aquaporin-4 (AQP4) immunoreactivity in the piriform cortex (PC) and the hippocampus of pilocarpine-induced rat epilepsy model to elucidate the roles of AQP4 in brain edema following status epilepticus (SE)...
  46. ncbi Recurrent circuits in layer II of medial entorhinal cortex in a model of temporal lobe epilepsy
    Sanjay S Kumar
    Department of Comparative Medicine, Stanford University, Stanford, California 94305, USA
    J Neurosci 27:1239-46. 2007
    ..reorganization and formation of recurrent, excitatory synapses among layer II stellate cells in epileptic pilocarpine-treated rats...
  47. pmc The CREB/CRE transcriptional pathway: protection against oxidative stress-mediated neuronal cell death
    Boyoung Lee
    Department of Neuroscience, Ohio State University, Columbus, 43210, USA
    J Neurochem 108:1251-65. 2009
    ..Using the pilocarpine-evoked repetitive seizure model, we detected a marked enhancement of cell death in A-CREB transgenic mice...
  48. ncbi Local cerebral blood flow during lithium-pilocarpine seizures in the developing and adult rat: role of coupling between blood flow and metabolism in the genesis of neuronal damage
    Anne Pereira de Vasconcelos
    Faculty of Medicine, Institut National de la Sante et de la Recherche Medicale, Strasbourg, France
    J Cereb Blood Flow Metab 22:196-205. 2002
    ..Lithium-pilocarpine-induced status epilepticus is associated with extended damage in adult rats, mostly in the forebrain limbic ..
  49. ncbi Electroacupuncture prevents cognitive deficits in pilocarpine-epileptic rats
    Jair Guilherme dos Santos
    Department of Physiology, Universidade Federal de Sao Paulo, Rua Botucatu, 862, 5 andar, Ed Ciencias Biomedicas, 04023 062 Sao Paulo, Brazil
    Neurosci Lett 384:234-8. 2005
    Here we investigated the effects of electroacupuncture over the cognitive deficits in the pilocarpine model of epilepsy in rats...
  50. ncbi Variations in elemental compositions of rat hippocampal formation between acute and latent phases of pilocarpine-induced epilepsy: an X-ray fluorescence microscopy study
    J Chwiej
    Department of Medical Physics and Biophysics, Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland
    J Biol Inorg Chem 17:731-9. 2012
    ..used experimental model of the temporal lobe epilepsy observed in clinical practice is the one based on pilocarpine-induced seizures...
  51. ncbi Alteration of purinergic P2X4 and P2X7 receptor expression in rats with temporal-lobe epilepsy induced by pilocarpine
    Flavia Dona
    Departamento Neurologia Neurocirurgia, Universidade Federal de Sao Paulo, UNIFESP, Sao Paulo, Brazil
    Epilepsy Res 83:157-67. 2009
    ..Temporal-lobe epilepsy (TLE) in rat was induced by pilocarpine in order to study changes of hippocampal P2X(2), P2X(4) and P2X(7) receptor expression during acute, latent or ..
  52. ncbi Activation of cerebral peroxisome proliferator-activated receptors gamma exerts neuroprotection by inhibiting oxidative stress following pilocarpine-induced status epilepticus
    Xin Yu
    Research Center of Epilepsy, Department of Neurology, Xijing Hospital Fourth Military Medical University, 17 Changle West Road, Xi an, Shaanxi 710032, China
    Brain Res 1200:146-58. 2008
    ..Using a lithium-pilocarpine-induced SE model, we found that rosiglitazone significantly reduced hippocampal neuronal loss 1 week after SE, ..
  53. pmc Redistribution of CB1 cannabinoid receptors in the acute and chronic phases of pilocarpine-induced epilepsy
    Mária R Karlócai
    Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
    PLoS ONE 6:e27196. 2011
    ..Using the pilocarpine model of temporal lobe epilepsy we examined the expression pattern of the Type 1 cannabinoid receptor (CB1-R) ..
  54. ncbi Glial activation in a pilocarpine rat model for epileptogenesis: a morphometric and quantitative analysis
    Felipe S Estrada
    Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico
    Neurosci Lett 514:51-6. 2012
    In this work we examined the correlation between long-term glial resilience and slow epileptogenesis using the pilocarpine-insult rat model...
  55. ncbi Role of signal transducer and activator of transcription-3 in up-regulation of GFAP after epilepsy
    Zucai Xu
    Department of Neurology, The Second Affiliated Hospital, Chongqing Medical University, Lin Jiang Road 74, Chongqing 400010, China
    Neurochem Res 36:2208-15. 2011
    ..These findings indicate that epilepsy involves the activation of STAT3 that up-regulates the expression of GFAP, which may play an important role in epileptogenesis...
  56. ncbi Cellular and network properties of the subiculum in the pilocarpine model of temporal lobe epilepsy
    Andreas Knopp
    Neuroscience Research Center of the Charite, Humboldt University of Berlin, D 10117 Berlin, Germany
    J Comp Neurol 483:476-88. 2005
    ..Using the pilocarpine model of epilepsy, this study examines the anatomical substrates for network hyperexcitability recorded in the ..
  57. ncbi Evaluation of the antiepileptic effect of curcumin and Nigella sativa oil in the pilocarpine model of epilepsy in comparison with valproate
    Neveen A Noor
    Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
    Epilepsy Behav 24:199-206. 2012
    ..and Nigella sativa oil (NSO) on amino acid neurotransmitter alterations and the histological changes induced by pilocarpine in the hippocampus and cortex of rats. Epilepsy was induced by i.p...
  58. ncbi Neuronal and glial pathological changes during epileptogenesis in the mouse pilocarpine model
    Karin Borges
    Department of PharmacologyEmory University, Atlanta, GA 30322, USA
    Exp Neurol 182:21-34. 2003
    The rodent pilocarpine model of epilepsy exhibits hippocampal sclerosis and spontaneous seizures and thus resembles human temporal lobe epilepsy...
  59. ncbi Time-course of neuronal death in the mouse pilocarpine model of chronic epilepsy using Fluoro-Jade C staining
    Lian Wang
    Institute of Neurosciences, The Fourth Military Medical University, Xi an 710032, PR China
    Brain Res 1241:157-67. 2008
    ..injury that may occur in the temporal lobe epilepsy, and time-course of neuronal death was examined in a mouse pilocarpine model of chronic epilepsy by Fluoro-Jade C (FJC) dye that can specifically stain the degenerative neurons in ..
  60. ncbi Enhanced proliferation of acinar and progenitor cells by prophylactic pilocarpine treatment underlies the observed amelioration of radiation injury to parotid glands
    Fred R Burlage
    Department of Radiation Oncology, University Medical Center Groningen, The Netherlands
    Radiother Oncol 90:253-6. 2009
    Administration of pilocarpine before irradiation can ameliorate radiation-induced hyposalivation. Indirect evidence suggests that this effect may be mediated through induction of a compensatory response...
  61. pmc A cellular mechanism for dendritic spine loss in the pilocarpine model of status epilepticus
    Jonathan E Kurz
    Department of Neurology, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    Epilepsia 49:1696-710. 2008
    ..CaN has been shown to induce dephosphorylation of cofilin, an actin depolymerization factor. The ensuing actin depolymerization can lead to a number of physiological changes that are of interest in SE...
  62. pmc Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats
    Hai Ju Zhang
    Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan 430060, China
    J Zhejiang Univ Sci B 9:903-15. 2008
    To examine modulations caused by cyclooxygenase-2 (COX-2) inhibitors on altered microenvironments and overbalanced neurotransmitters in pilocarpine-induced epileptic status rats and to investigate possible mechanisms.
  63. ncbi Reactive oxygen species generated by NADPH oxidase are involved in neurodegeneration in the pilocarpine model of temporal lobe epilepsy
    Rafaela R F Pestana
    Department of Physiology and Biophysics, University of Sao Paulo, Sao Paulo, SP 05508 900, Brazil
    Neurosci Lett 484:187-91. 2010
    ..stress could have a role in the hippocampal neurodegeneration observed in temporal lobe epilepsy induced by pilocarpine. We first determined the spatio-temporal pattern of ROS generation, by means of detection with dihydroethidium ..
  64. pmc Disruption of the m1 receptor gene ablates muscarinic receptor-dependent M current regulation and seizure activity in mice
    S E Hamilton
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195 7750, USA
    Proc Natl Acad Sci U S A 94:13311-6. 1997
    ..mutant mice are highly resistant to the seizures produced by systemic administration of the muscarinic agonist pilocarpine. Thus, the m1 receptor subtype mediates M current modulation in sympathetic neurons and induction of seizure ..
  65. ncbi Transcranial direct current stimulation decreases convulsions and spatial memory deficits following pilocarpine-induced status epilepticus in immature rats
    Tohru Kamida
    Department of Neurosurgery, Oita University Faculty of Medicine, Hasama machi, Oita 879 5593, Japan
    Behav Brain Res 217:99-103. 2011
    ..The effects of cathodal tDCS on convulsions and spatial memory after status epilepticus (SE) in immature animals were investigated...
  66. ncbi Prolonged seizures increase proliferating neuroblasts in the adult rat subventricular zone-olfactory bulb pathway
    Jack M Parent
    Department of Neurology, University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA
    J Neurosci 22:3174-88. 2002
    ..Within 1-2 weeks after pilocarpine-induced SE, bromodeoxyuridine (BrdU) labeling and Nissl staining increased in the rostral forebrain SVZ...
  67. pmc Hippocampal interictal spikes disrupt cognition in rats
    Jonathan K Kleen
    Department of Neurology, Neuroscience Center at Dartmouth, Dartmouth Medical School, Hanover, New Hampshire
    Ann Neurol 67:250-7. 2010
    ..We investigated the transient impact of focal IISs on the hippocampus, a structure crucial for learning and memory and yet highly prone to IISs in temporal lobe epilepsy (TLE)...
  68. pmc Validation of suitable reference genes for expression studies in different pilocarpine-induced models of mesial temporal lobe epilepsy
    Thalita Ewellyn Batista Sales Marques
    Department of Cell, Molecular Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, AL, Brazil
    PLoS ONE 8:e71892. 2013
    ..We investigated eight potential reference genes in two different pilocarpine PILO-models of mesial temporal lobe epilepsy (MTLE) performing a stability expression analysis using geNorm, ..
  69. pmc Whole transcriptome analysis of the hippocampus: toward a molecular portrait of epileptogenesis
    Oswaldo K Okamoto
    Disciplina de Neurologia Experimental, Departamento de Neurologia e Neurocirurgia, Universidade Federal de Sao Paulo
    BMC Genomics 11:230. 2010
    ....
  70. ncbi Oxidative stress in rat hippocampus caused by pilocarpine-induced seizures is reversed by buspirone
    Rizângela Lyne Mendes de Freitas
    Course of Pharmacy of Catholic Faculty Rainha do Sertão, Quixada, Ceara, CE, Brazil
    Brain Res Bull 81:505-9. 2010
    ..buspirone effects on oxidative stress in rat hippocampus after seizures and status epilepticus (SE) induced by pilocarpine. In pilocarpine group there was a significant increase in lipid peroxidation and nitrite levels...
  71. ncbi A 5-month period of epilepsy impairs spatial memory, decreases anxiety, but spares object recognition in the lithium-pilocarpine model in adult rats
    Julien Detour
    INSERM U398, Faculty of Medicine, Strasbourg, France
    Epilepsia 46:499-508. 2005
    In temporal lobe epilepsy (TLE), interictal behavioral disorders affect patients' quality of life. Therefore we studied long-term behavioral impairments in the lithium-pilocarpine (li-pilo) model of TLE.
  72. pmc Altered expression and function of small-conductance (SK) Ca(2+)-activated K+ channels in pilocarpine-treated epileptic rats
    Mauro S Oliveira
    Departamento de Fisiologia e Farmacologia, Centro de Ciencias da Saude, Universidade Federal de Santa Maria, RS, Brazil
    Brain Res 1348:187-99. 2010
    ..In this study, we investigate the expression and function of SK channels in the pilocarpine model of mesial temporal lobe epilepsy...
  73. pmc Pilocarpine-induced temporal lobe epilepsy in the rat is associated with increased dopamine neuron activity
    Pierangelo Cifelli
    Departments of Neuroscience, Psychiatry and Psychology, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Int J Neuropsychopharmacol 15:957-64. 2012
    ..of temporal lobe seizures, we investigated whether an animal model of TLE using intrahippocampal injection of pilocarpine induces alterations in mesolimbic dopamine neuron activity...
  74. pmc High ratio of synaptic excitation to synaptic inhibition in hilar ectopic granule cells of pilocarpine-treated rats
    Ren Zhi Zhan
    Dept of Pharmacology and Cancer Biology, Box 3813, Duke Univ Medical Center, Durham, NC 27710, USA
    J Neurophysiol 104:3293-304. 2010
    ..This study used hippocampal slices prepared from rats that had experienced pilocarpine-induced status epilepticus to test the hypothesis that an imbalance of synaptic excitation and inhibition ..
  75. pmc P2X7 receptor activation ameliorates CA3 neuronal damage via a tumor necrosis factor-α-mediated pathway in the rat hippocampus following status epilepticus
    Ji Eun Kim
    Department of Anatomy and Neurobiology, Institute of Epilepsy Research, College of Medicine, Hallym University, Chunchon, Kangwon Do 200 702, South Korea
    J Neuroinflammation 8:62. 2011
    ..In the present study, we addressed the question of whether P2X7 receptor-mediated TNF-α regulation is involved in pathogenesis and outcome of status epilepticus (SE)...
  76. ncbi Comparison of status epilepticus models induced by pilocarpine and nerve agents - a systematic review of the underlying aetiology and adopted therapeutic approaches
    F R Tang
    Temasek Laboratories, National University of Singapore, Singapore
    Curr Med Chem 18:886-99. 2011
    ..and metabolic, biochemical, neuroinflammatory changes and current therapeutic approaches reported in pilocarpine, soman, and sarin models of seizures...
  77. ncbi Changes in vesicular transporters for gamma-aminobutyric acid and glutamate reveal vulnerability and reorganization of hippocampal neurons following pilocarpine-induced seizures
    Jean Luc Boulland
    The Biotechnology Centre of Oslo, University of Oslo, Oslo, N 0349 Norway
    J Comp Neurol 503:466-85. 2007
    ..GABA)-ergic hippocampal networks as well as the time course of these reorganizations during development of pilocarpine-induced temporal lobe epilepsy were studied with in situ hybridization and immunohistochemistry experiments for ..
  78. pmc Stereological methods reveal the robust size and stability of ectopic hilar granule cells after pilocarpine-induced status epilepticus in the adult rat
    Daniel P McCloskey
    Center for Neural Recovery and Rehabilitation Research, Helen Hayes Hospital, Route 9W, West Haverstraw, NY 10993, USA
    Eur J Neurosci 24:2203-10. 2006
    ..total number of ectopic hilar granule cells was estimated using unbiased stereology at different times after pilocarpine-induced status epilepticus...
  79. ncbi Antioxidant response and oxidative damage in brain cortex after high dose of pilocarpine
    S Tejada
    Laboratori de Neurofisiologia, Departament de Biologia Fonamental i Ciencies de la Salut, Universitat de les Illes Balears, Crta Valldemossa, km 7 5, E 07122 Palma de Mallorca, Balears, Spain
    Brain Res Bull 71:372-5. 2007
    b>Pilocarpine is a cholinergic agonist capable to induce seizures and an epilepticus-like state in rodents...
  80. ncbi mGluR5-PLCbeta4-PKCbeta2/PKCgamma pathways in hippocampal CA1 pyramidal neurons in pilocarpine model of status epilepticus in mGluR5+/+ mice
    Jian Xin Liu
    Epilepsy Research Laboratory, National Neuroscience Institute, Singapore, Singapore
    Epilepsy Res 82:111-23. 2008
    ..PKCbeta2 or PKCgamma expression at the border between the stratum oriens and alveus (O/A border) at 2h during pilocarpine induced status epilepticus (SE), and in the stratum pyramidale in CA1 area at 1 day after pilocarpine induced ..
  81. ncbi The duration of sustained convulsive seizures determines the pattern of hippocampal neurogenesis and the development of spontaneous epilepsy in rats
    Yu Wen Hung
    Institute of Physiology, National Yang Ming University, Taipei, Taiwan
    Epilepsy Res 98:206-15. 2012
    ..Sprague-Dawley rats with pilocarpine-induced convulsive SS were divided into short SS (30 min) and long SS (2 h) groups...
  82. ncbi Seizure susceptibility and the brain regional sensitivity to oxidative stress in male and female rats in the lithium-pilocarpine model of temporal lobe epilepsy
    Sandra Peternel
    Department of Pharmacology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia
    Prog Neuropsychopharmacol Biol Psychiatry 33:456-62. 2009
    ..of different brain regions to oxidative stress in rats with status epilepticus (SE) induced by lithium-pilocarpine administration, that provides a common experimental model of temporal lobe epilepsy (TLE) in humans...
  83. pmc Morphologic integration of hilar ectopic granule cells into dentate gyrus circuitry in the pilocarpine model of temporal lobe epilepsy
    Michael C Cameron
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Comp Neurol 519:2175-92. 2011
    After pilocarpine-induced status epilepticus, many granule cells born into the postseizure environment migrate aberrantly into the dentate hilus...
  84. ncbi The neuroprotective effect of curcumin and Nigella sativa oil against oxidative stress in the pilocarpine model of epilepsy: a comparison with valproate
    Heba S Aboul Ezz
    Laboratory of Neurophysiology, Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
    Neurochem Res 36:2195-204. 2011
    Oxidative stress has been implicated to play a role in epileptogenesis and pilocarpine-induced seizures...
  85. ncbi Septal GABAergic neurons are selectively vulnerable to pilocarpine-induced status epilepticus and chronic spontaneous seizures
    E R Garrido Sanabria
    Department of Biological Sciences, University of Texas at Brownsville Texas Southmost College, 80 Fort Brown, Brownsville, TX 78520, USA
    Neuroscience 142:871-83. 2006
    ..b>Pilocarpine-induced status epilepticus (SE) was chosen as a model to generate chronic epileptic animals...
  86. ncbi Ketogenic diet exhibits neuroprotective effects in hippocampus but fails to prevent epileptogenesis in the lithium-pilocarpine model of mesial temporal lobe epilepsy in adult rats
    Benjamin Linard
    INSERM U666, Université de Strasbourg Strasbourg, France
    Epilepsia 51:1829-36. 2010
    ..Our goal was to assess the neuroprotective or antiepileptogenic effect of these diets in a well-characterized model of mesial temporal lobe epilepsy following initial SE induced by lithium-pilocarpine in adult rats.
  87. ncbi Effect of changing from concomitant timolol pilocarpine to bimatoprost monotherapy on ocular blood flow and IOP in primary chronic angle closure glaucoma
    Harish C Agarwal
    Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India
    J Ocul Pharmacol Ther 19:105-12. 2003
    ..pressure (IOP) in eyes of primary chronic angle closure glaucoma patients already on concomitant timolol and pilocarpine. Thirty two patients of bilateral primary chronic angle closure glaucoma (PCACG) on topical timolol 0...
  88. pmc Elevated plasma corticosterone level and depressive behavior in experimental temporal lobe epilepsy
    Andrey M Mazarati
    Department of Pediatrics, Neurology Division, David Geffen School of Medicine, University of California Los Angeles, Box 951752, 22 474 MDCC, Los Angeles, CA 90095 1752, USA
    Neurobiol Dis 34:457-61. 2009
    ..We suggest that the observed hyperactivity of the HPA axis may underlie co-morbidity between epilepsy and depression...
  89. ncbi Strain differences affect the induction of status epilepticus and seizure-induced morphological changes
    B Xu
    Department of Psychology, McMaster University, 1280 Main Street West, Hamilton, ON, Canada L8S 4K1
    Eur J Neurosci 20:403-18. 2004
    ..We conclude that genetic factors clearly affect seizure development and related morphological changes in both standard laboratory strains and the selectively bred seizure-prone and seizure-resistant strains...
  90. ncbi The role of muscarinic acetylcholine receptor-mediated activation of extracellular signal-regulated kinase 1/2 in pilocarpine-induced seizures
    Jennifer L Berkeley
    Department of Neurology, Center for Neurodegenerative Disease, Emory University, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA 30322, USA
    J Neurochem 82:192-201. 2002
    b>Pilocarpine-induced seizures are mediated by the M(1) subtype of muscarinic acetylcholine receptor (mAChR), but little is known about the signaling mechanisms linking the receptor to seizures...
  91. ncbi The effects of alpha-tocopherol on hippocampal oxidative stress prior to in pilocarpine-induced seizures
    A R Tome
    State University of Ceara, Campus do Itaperi, Fortaleza, Ceara, Brazil
    Neurochem Res 35:580-7. 2010
    ..It has been suggested that pilocarpine-induced seizures is mediated by an increase in oxidative stress...
  92. ncbi Selective changes in single cell GABA(A) receptor subunit expression and function in temporal lobe epilepsy
    A R Brooks-Kayal
    Joseph Stokes Research Institute of The Children s Hospital of Philadelphia, Department of Neurology, University of Pennsylvania, 19104, USA
    Nat Med 4:1166-72. 1998
    ....
  93. ncbi Reduced inhibition of dentate granule cells in a model of temporal lobe epilepsy
    Masayuki Kobayashi
    Department of Comparative Medicine, Stanford University School of Medicine, Stanford, California 94305 5342, USA
    J Neurosci 23:2440-52. 2003
    ..We report the loss of GABAergic inhibition of granule cells in the temporal dentate gyrus of pilocarpine-induced epileptic rats...
  94. ncbi Loss of GABAergic neurons in the subiculum and its functional implications in temporal lobe epilepsy
    Andreas Knopp
    Dept of Psychiatry and Psychotherapy, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Brain 131:1516-27. 2008
    ..Using the pilocarpine-model of temporal lobe epilepsy (TLE), the present study examines the vulnerability of GABAergic subicular ..
  95. ncbi Increased persistent sodium currents in rat entorhinal cortex layer V neurons in a post-status epilepticus model of temporal lobe epilepsy
    Newton Agrawal
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada
    Epilepsia 44:1601-4. 2003
    ..In this study, we investigated whether status epilepticus caused changes in the function of voltage-gated sodium channels in entorhinal cortex layer V neurons, a cellular group important for the genesis of limbic seizures...
  96. ncbi Status epilepticus in immature rats leads to behavioural and cognitive impairment and epileptogenesis
    Hana Kubova
    Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeòská 1083, Prague 4, CZ 142 20, Czech Republic
    Eur J Neurosci 19:3255-65. 2004
    ..The present study examined whether SE induced by LiCl-pilocarpine in normal immature brain (at the age of 12 or 25 days; P12 or P25) causes cognitive decline and epileptogenesis,..
  97. pmc Comorbidity between epilepsy and depression: experimental evidence for the involvement of serotonergic, glucocorticoid, and neuroinflammatory mechanisms
    Eduardo Pineda
    Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1752, USA
    Epilepsia 51:110-4. 2010
    ..We established that animals that had been subjected to lithium chloride and pilocarpine status epilepticus (SE) and developed spontaneous recurrent seizures, exhibited a set of impairments congruent ..
  98. ncbi Long-term expressional changes of Na+ -K+ -Cl- co-transporter 1 (NKCC1) and K+ -Cl- co-transporter 2 (KCC2) in CA1 region of hippocampus following lithium-pilocarpine induced status epilepticus (PISE)
    Xiubin Li
    Neurological Department of the 1st Affiliated Hospital of Sun Yat sen University, Guangzhou, 510080, PR China
    Brain Res 1221:141-6. 2008
    ..to investigate the long-term expression profiles of NKCC1 and KCC2 in CA1 region in the mice model of lithium-pilocarpine induced status epilepticus (PISE) and their relationship with epileptogenesis...
  99. ncbi An increase in persistent sodium current contributes to intrinsic neuronal bursting after status epilepticus
    Shmuel Chen
    Department of Medical Neurobiology, Hebrew University Hadassah School of Medicine, P O Box 12272, Jerusalem 91121, Israel
    J Neurophysiol 105:117-29. 2011
    ..In the widely used pilocarpine-status epilepticus (SE) rat model of temporal lobe epilepsy (TLE), a major alteration is the marked increase in ..
  100. ncbi Proteome changes associated with hippocampal MRI abnormalities in the lithium pilocarpine-induced model of convulsive status epilepticus
    Nicholas D E Greene
    Neural Development Unit, UCL Institute of Child Health, University College London, London, UK
    Proteomics 7:1336-44. 2007
    ..The lithium pilocarpine model of status epilepticus (SE) in the rat provides a model in which to investigate the molecular and ..
  101. ncbi Effects of repeated electroconvulsive shock seizures and pilocarpine-induced status epilepticus on emotional behavior in the rat
    Armando Cardoso
    Faculty of Medicine of Porto, Institute of Anatomy, Alameda Prof Hernani Monteiro, Porto, Portugal
    Epilepsy Behav 14:293-9. 2009
    ..shock (ECS) and compared them with those resulting from prolonged status epilepticus (SE) induced with pilocarpine. Using the open-field and elevated plus-maze tests, we detected the presence of behavioral alterations ..

Research Grants63

  1. Prostaglandin signaling following seizures
    Jianxiong Jiang; Fiscal Year: 2013
    ..More recently, we have found that pharmacological inhibition of EP2 receptors after pilocarpine-induced status epilepticus in mice provides many beneficial effects including reductions in mortality, weight ..
  2. Mechanism and Treatment of nerve agent-induced seizures
    Jaideep Kapur; Fiscal Year: 2010
    ..Aim 3: To test anticonvulsant action of two classes of compounds in controlling lithium/pilocarpine and OP paraoxon-induced status epilepticus...
  3. Subunit dependent properties of kainate receptors
    David D Mott; Fiscal Year: 2013
    ..course of changes in KAR subunit RNA and protein in the hippocampus during the development of epilepsy after pilocarpine-induced status epilepticus (SE)...
  4. Mechanisms of comorbidity between epilepsy and depression
    Andrey M Mazarati; Fiscal Year: 2013
    ..We will employ a model of chronic epilepsy that develops in male Wistar rats following pilocarpine - induced status epilepticus (SE)...
  5. Neurobiological basis of Depression as a Comorbidity in Epilepsy
    Andrey M Mazarati; Fiscal Year: 2010
    ..To induce TLE, two weeks-old Wistar rats will be subjected to LiCl and pilocarpine status epilepticus (SE)...
  6. QUANTITATIVE GENETIC STUDY OF SEIZURES
    Thomas N Ferraro; Fiscal Year: 2012
    ..MEST QTLs are captured by testing them with chemoconvulsants pentylenetetrazol (PTZ), kainic acid (KA) and pilocarpine (PC). QTLs will be prioritized for further dissection based upon the results from all paradigms...
  7. Rational polytherapy in the treatment of cholinergic seizures
    Claude G Wasterlain; Fiscal Year: 2013
    ..combinations can, with little depression of consciousness, stop seizures induced by high-dose lithium and pilocarpine and refractory to a profoundly anesthetic dose of benzodiazepine, the best 2- and 3-drug combinations in a ..
  8. mTOR dysregulation in Epilepsy
    Angela N Carter; Fiscal Year: 2013
    ..The aims of this research proposal are: 1) to further characterize aberrant mTOR signaling in the pilocarpine model of epilepsy and evaluate mTORC1 inhibition using rapamycin 2) to evaluate whether mTOR hyperactivity ..
  9. Stem Cell Transplantation for Epilepsy
    JANICE RAE NAEGELE; Fiscal Year: 2013
    ..interneurons transplanted into the dentate gyrus of the hippocampus in the adult nervous system of mice with pilocarpine-induced TLE...
  10. Molecular Determinants of GABAA Receptor Gene Regulation
    SHELLEY JUNE RUSSEK; Fiscal Year: 2010
    ..mRNA levels in vivo underlie changes in functional properties of dentate granule cells following pilocarpine-induced status epilepticus (SE)...
  11. Canonical Transient Receptor Potential Channels and Excitotoxicity
    Fang Zheng; Fiscal Year: 2010
    ..To determine whether TRPC channels contribute to seizure and excitotoxicity in vivo, the well-established pilocarpine- induced limbic seizure model will be used...
  12. Neurosteroid Treatment for OP Intoxication
    Doodipala Samba Reddy; Fiscal Year: 2013
    ..evidence that late post-exposure neurosteroid therapy can rapidly control SE and be neuroprotective after SE in pilocarpine and DFP models, suggesting the promising efficacy of neurosteroid therapy...
  13. Cardiac dysfunction in epilepsy: a candidate mechanism in sudden unexpected death
    Anne E Anderson; Fiscal Year: 2012
    ..In the studies outlined we will utilize the pilocarpine model of acquired epilepsy that recapitulates a number of the features of chronic epilepsy in humans, including ..
  14. Treatment of Status Epilepticus: A Translational Proposal
    Claude G Wasterlain; Fiscal Year: 2013
    ..We will induce SE chemically with lithium and pilocarpine, or electrically by stimulating the perforant path, and will treat at 3 time points: after the second intense ..
  15. INTERNEURON BASED MECHANISMS OF TEMPORAL LOBE EPILEPSY
    Paul S Buckmaster; Fiscal Year: 2013
    ..numbers of release sites by recording basket cell-to-granule cell uIPSCs in slices from control and epileptic pilocarpine- treated rats...
  16. AGE RELATED SEIZURE SUPPRESSION
    Solomon L Moshe; Fiscal Year: 2011
    ..Male and female rats will be used. Methods include induction of SE using kainic acid or pilocarpine, intracranial microinfusions of GABAA agents and seizure testing using flurothyl or pentylenetetrazole, ..
  17. Epilepsy and dendritic excitability
    Nicholas P Poolos; Fiscal Year: 2013
    ..techniques to assay kinase and phosphatase activity, and long-term video-electroencephalography (VEEG) in the pilocarpine animal model of epilepsy...
  18. Altered phosphorylation of connexin 43 contributes to increased gap-junction coup
    HEIDI GRABENSTATTER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Pilocarpine-induced status epileptics (SE) induce reactive gliosis and activate calcium signaling in astrocytes of the hippocampus in a time-dependent manner (1, 2)...
  19. Perisomatic Inhibitory Network Dysfunction in Neurological Disease
    Vijayalakshmi Santhakumar; Fiscal Year: 2013
    ..The study will use pilocarpine induced status epilepticus to model development of acquired epilepsy, and a combination of anatomical, ..
  20. Rat transcriptome biomarkers for the prediction of temporal lobe epilepsy
    STANISLAV KARSTEN; Fiscal Year: 2009
    ..Male and female Wistar and Sprague-Dawley rats will be subjected to SE induced by either systemic pilocarpine or intrahippocampal kainic acid during either childhood (postnatal day 35) or adulthood (postnatal day 90)...
  21. Adenosine Receptors and Restoration of Salivary Gland in Sjogren's Syndrome
    Umesh S Deshmukh; Fiscal Year: 2013
    ..of SS will be treated with AR agonists and restoration of salivary gland function monitored by measuring pilocarpine induced salivation...
  22. Mechanisms of Cognitive Impairment in Temporal Lobe Epilepsy
    Barbara Jobst; Fiscal Year: 2013
    ..has recently pursued the underlying mechanisms responsible for dynamic cognitive dysfunction in the lithium-pilocarpine model of TLE finding that interictal spikes (IIS), dysfunctions in network oscillation activity (EEG rhythms) ..
  23. Anticonvulsant effects of novel concentric ring electrode electrical stimulation
    WALTER GRANT BESIO; Fiscal Year: 2010
    ..Hypothesis 2: {The suppression of pilocarpine (PILO)-induced status epilepticus (SE) by TcES via concentric ring electrodes reduces SE-provoked neuronal ..
  24. Neuronal Loss and Plasticity in Epilepsy
    Carolyn R Houser; Fiscal Year: 2013
    ..chloride transporters will be altered in ways that could reduce the effectivenss of GABAergic inhibition in the pilocarpine-treated animals and thus contribute to the development of epilepsy and the initiation of spontaneous seizures...
  25. STATUS EPILEPTICUS REORGANIZES CANNABINOID RECEPTORS
    ROBERT JOHN DELORENZO; Fiscal Year: 2010
    ..Our laboratory has demonstrated that one hour of prolonged seizures (status epilepticus, SE) in the pilocarpine model of SE produces a long-term reorganization in the expression of the CB1 receptor ahd that these changes in ..
  26. Seizure-induced Neuronal Loss and Epileptogenesis in the Immature Brain
    JEFFREY JOHN EKSTRAND; Fiscal Year: 2013
    ..This project utilizes the lithium pilocarpine (LiPC) animal model of epilepsy, a well established model of chemoconvulsant status epilepticus (SE), to ..
  27. Epilepsy and dendritic excitability
    NICHOLAS POOLOS; Fiscal Year: 2009
    ..techniques to assay kinase and phosphatase activity, and long-term video-electroencephalography (VEEG) in the pilocarpine animal model of epilepsy...
  28. Genetics of Mouse Seizure Susceptibility
    Melodie Rose Winawer; Fiscal Year: 2013
    ..A CSS) to identify QTLs on five chromosomes that confer increased susceptibility to pilocarpine-induced limbic seizures, a rodent model of human temporal lobe epilepsy...
  29. Genetic Analysis of a Rodent Model of Epilepsy and Depression Co-morbidity
    SUNSHINE ALISHA EPPS; Fiscal Year: 2012
    ..trait loci analysis will be used to map activity in the forced swim test and latency to seizure following pilocarpine administration to distinct chromosomal regions harboring genes responsible for the depression and epilepsy-like ..
  30. Aberrant Neurogenesis in Experimental Temporal Lobe Epilepsy
    Jack M Parent; Fiscal Year: 2013
    ..Using the rat pilocarpine epilepsy model, we recently discovered that only developing, and not mature, DGCs are responsible for abnormal ..
  31. PG-INDUCED REDUCTION OF INTRAOCULAR PRESSURE
    Carl Camras; Fiscal Year: 1993
    ..The effects of cyclo- oxygenase inhibitors (COIs) on the IOP response after epinephrine, timolol, pilocarpine phospholine iodide, arachidonic acid, PGF2 alpha, forskolin, vanadate, corynanthine, pergolide, salbutamol, and ..
  32. Dentate Gyrus Circuitry In Temporal Lobe Epilepsy
    Paul S Buckmaster; Fiscal Year: 2013
    ..mossy cells enlarge, elongate dendritic processes, and receive more excitatory synaptic input in epileptic pilocarpine-treated rats. Hippocampal slices, whole-cell patch recording, and biocytin labeling will be used...
  33. Role of COX2 of Neuronal Origin in Blood-Brain Communication after status epilept
    Raymond J Dingledine; Fiscal Year: 2012
    ..A comparison of the pilocarpine and kainate SE models will be done to minimize model-specific conclusions...
  34. Regulation of epileptogenesis by the transcriptional repressor, REST
    Jenny Hsieh; Fiscal Year: 2013
    ..A comparison of the pilocarpine and kainate SE models will be done to minimize model-specific conclusions...
  35. Prostanoid Modulators that Reduce Brain Injury After Seizures
    Raymond J Dingledine; Fiscal Year: 2013
    ..Prolonged status epilepticus (SE) induced by the muscarinic agonist, pilocarpine, or the organophosphate, diisopropyl fluorophosphate (DFP), triggers a similar series of cellular events in the ..
  36. Evaluation of Neuroprotective Effects of AEOL10150 against Chemical Threat Agents
    Manisha N Patel; Fiscal Year: 2012
    ..profile of AEOL10150 in rats (Specific Aim 1) and evaluate the neuroprotective efficacy of AEOL10150 against pilocarpine-induced neurotoxicity in rats (Specific Aim 2) using a variety of biochemical, pharmacological and analytical ..
  37. PHARMACOTHERAPY FOR XEROSTOMIA IN SJOGREN'S SYNDROME
    SAMUEL HOPKINS; Fiscal Year: 2005
    ..including tear and saliva substitutes and centrally acting parasympathomimetic secretagogues such as pilocarpine and cevimeline, provide transient relief, but patients often find these remedies costly, ineffective, ..
  38. Cognitive impact of iterictal spikes in temporal lobe epilepsy
    JONATHAN KLEEN; Fiscal Year: 2013
    ..Aims: 1) Investigate the neural circuitry of interictal spike genesis and propagation in the intrahippocampal-pilocarpine model of temporal lobe epilepsy...
  39. Low-Frequency Sine-Wave Neurostimulation Therapy for Epilepsy
    Jeffrey H Goodman; Fiscal Year: 2013
    ..of LFSWS parameters, and the efficacy and safety testing of LFSWS using the rodent tetanus toxin and pilocarpine spontaneous seizure models...
  40. New Electrographic Biomarkers of Epileptogenesis
    Anatol Bragin; Fiscal Year: 2013
    ..We will focus our efforts on the analysis of the UDS electrographic pattern prior to pilocarpine induced status epilepticus and compare it to activity recorded during the latent period before spontaneous ..
  41. Seizures Epileptogenesis and TrkB Transactivation
    James O McNamara; Fiscal Year: 2013
    ..of the hippocampal mossy fiber-CA3 pyramid synapse in slices ex vivo;and epileptogenesis in the kindling and pilocarpine models in vivo...
  42. Neuroactive Steroid Therapy of Catamenial Epilepsy
    DOODIPALA REDDY; Fiscal Year: 2007
    ..We will critically test this hypothesis using a rat pilocarpine model of temporal lobe epilepsy with SRS...
  43. MECHANISM OF CANNABINOID ANTI-CONVULSANT EFFECTS
    ROBERT JOHN DELORENZO; Fiscal Year: 2011
    Our laboratory has demonstrated that the induction of epilepsy in the pilocarpine model of acquired epilepsy produces an essentially permanent neuronal plasticity change in the expression of the cannabinoid receptor (CB1), one of the ..
  44. GABA-A Receptor Gene Transfer to Prevent Epileptogenesis
    SHELLEY RUSSEK; Fiscal Year: 2004
    ..Little is known, however, about the genetic mechanisms that regulate granule cells of adult rats following pilocarpine-induced status epilepticus (SE), it has yet to be demonstrated that these changes are either necessary or ..
  45. Hippocampal Interneuron Network Dynamics After Epileptogenesis
    PEYMAN GOLSHANI; Fiscal Year: 2013
    ..Here we propose to use the pilocarpine model of TLE in combination with juxtacellular recording and biocytin labeling, in awake behaving mice ..
  46. ENDOGENOUS DYNORPHIN OPIOIDS AND TEMPORAL LOBE EPILEPSY
    Charles Chavkin; Fiscal Year: 1999
    ..b>Pilocarpine-treatment provides an animal model of temporal lobe epilepsy that duplicates many features of human pathology ..
  47. NEUROPHYSIOLOGICAL STUDY OF CA1 SYNAPTIC REORGANIZATION
    JOSE CAVAZOS; Fiscal Year: 2003
    ..We evaluated the Pilocarpine model, and we determined that although CA1 synaptic reorganization occurs, it was not robust enough for the ..
  48. REGULATION OF GLANDULAR MUCOUS CELL SECRETION
    DAVID CULP; Fiscal Year: 2001
    ..Current treatment for hyposalivation includes the systemic application of a muscarinic cholinergic agonist, pilocarpine, to directly stimulate remaining functional salivary secretory cells...
  49. CHOLINERGIC MECHANISMS IN AGING AND AD
    LINCOLN POTTER; Fiscal Year: 2002
    ..use by itself or in right hemi-PD, but is expected to alter responses to apomorphine (dopamine agonist), pilocarpine and xanomeline (muscarinic agonists)...
  50. ANTERIOR CHAMBER FLUID ACETYLCHOLINE CONCENTRATION
    Jonathan Wirtschafter; Fiscal Year: 1980
    ..endogenous ACh concentration in the aqueous humor is of the same order of magnitude as the average therapeutic pilocarpine concentration achieved in the anterior chamber with topical application...
  51. A NEW CHLORIDE SWEAT TEST FOR CYSTIC FIBROSIS SCREENING
    WARREN WARWICK; Fiscal Year: 2004
    ..stimulation method and collection cycle time will be optimized by comparing three new methods of transporting pilocarpine to the sweat glands and comparing the results to the current iontophoresis method...
  52. Muscarinic regulation of plasticity in the brain
    Gene Robinson; Fiscal Year: 2009
    ..is based on the surprising, but robust, demonstration that treatment of caged bees with a muscarinic agonist, pilocarpine, results in brain plasticity identical to that produced by a week of real foraging experience. We will: 1...
  53. Mechanisms of Limbic Epileptogenesis
    James O McNamara; Fiscal Year: 2010
    ..To determine whether inhibiting TrkB signaling prevents epileptogenesis in the pilocarpine status epilepticus model. To determine whether inhibiting TrkB signaling prevents epileptogenesis in the Kv1...
  54. LITHIUM POTENTIATED STATUS EPILEPTICUS
    RICHARD JOPE; Fiscal Year: 1990
    ..The basic model entails treatment with LiCl (3 mEq/kg; ip) and pilocarpine (30 mg/kg; sc) which invariably produces generalized convulsive status epilepticus which lasts for several ..
  55. TRANSCORNEAL FLUX ENHANCEMENT OF PILOCARPINE
    DAVID KROHN; Fiscal Year: 1980
    This proposal is to investigate kinetics of transcorneal flux and intra-ocular fate of pilocarpine. Methodology and mechanism of flux enhancement is of prime import...
  56. ADRENERGIC & CHOLINERGIC MECHANISMS IN GLAUCOMA THERAPY
    JON POLANSKY; Fiscal Year: 1993
    ..considerable interest in the mechanisms by which adrenergic (eg, epinephrine and timolol) and cholinergic (eg, pilocarpine, carbachol, echothiophate iodide) drugs reduce IOP, as well as to understand the effects of new alpha ..
  57. Signaling mechanisms underlying VEGF-induced neuroprotection in vivo
    Susan Croll; Fiscal Year: 2007
    ..VEGF also protects neurons in models of cell death in vivo. One such model is the pilocarpine-induced status epilepticus model, in which cell damage occurs within days after a single, prolonged status ..
  58. EVALUATION OF ESTERASE ACTIVITY AND PRODRUGS IN THE EYE
    Vincent Lee; Fiscal Year: 1993
    ..changes of 0-1'- methylcyclopropanoyl timolol upon co-administration with other glaucoma drugs such as pilocarpine, epinephrine, and their prodrugs, or with other ocular drugs such as phenylephrine and proparacaine...
  59. LONG TERM EFFECTS OF SEIZURES ON THE DEVELOPING BRAIN
    Gregory Holmes; Fiscal Year: 2002
    ..Using kainic acid, pilocarpine, and continuous hippocampal stimulation we have demonstrated that prolonged seizures in rats below 20 days of ..
  60. ROLE OF ENTOPEDUNCULAR NUCLEUS IN KINDLED SEIZURES
    David Hosford; Fiscal Year: 1991
    ..Preliminary evidence supports this hypothesis in the pilocarpine model of seizures...
  61. PHARMACOTHERAPRY FOR XEROSTOMIA IN SJOGREN'S SYNDROME
    KARL DONN; Fiscal Year: 2009
    ..for xerostomia, including saliva substitutes and centrally acting parasympathomimetic secretagogues such as pilocarpine and cevimeline, provide transient relief, but patients often find these remedies costly, ineffective, ..
  62. Investigating a Role for Glutamate Transport in Epilepsy
    Margaret Sutherland; Fiscal Year: 2006
    ..To test this central hypothesis, we will use three models of status epilepticus (KA, pilocarpine and kindling)...
  63. PILOCARPINE PRODRUGS WITH IMPROVED BIOAVAILABILITY
    TROY REAVES; Fiscal Year: 1990
    ..This proposal suggests the development of prodrugs of pilocarpine. These prodrugs are expected to reduce the elevated IOP which is associated with glaucoma and responsible for ..