Genomes and Genes
Summary: A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
Publications267 found, 100 shown here
- Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degenerationL C Schmued
Division of Neurotoxicology, National Center for Toxicological Research FDA, 3900 NCTR Road, 72079 9502, Jefferson, AR 72079 9502, USA
Brain Res 874:123-30. 2000..Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques...
- Effect of Leucine and Lysine substitution on the antimicrobial activity and evaluation of the mechanism of the HPA3NT3 analog peptideRamamourthy Gopal
Research Center for Proteineous Materials RCPM, Chosun University, Gwangju, Korea
J Pept Sci 15:589-94. 2009..These results suggest that the substitutions of Arg and Asn with Lys and two Trp with Leu resulted in small changes in HPA3NT3-analog activity and significant decreases in hemolytic activity...
- Comparing bacterial membrane interactions of antimicrobial peptides and their mimicsNathaniel P Chongsiriwatana
Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
Methods Mol Biol 618:171-82. 2010..By comparing the membrane interactions of AMPs and their mimics, these techniques can provide insights into their extent of mimicry and their antimicrobial mechanisms...
- In vitro P-glycoprotein assays to predict the in vivo interactions of P-glycoprotein with drugs in the central nervous systemBo Feng
Pharmacokinetics, Dynamics, and Metabolism Department, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
Drug Metab Dispos 36:268-75. 2008....
- Negative regulation of neural stem/progenitor cell proliferation by the Pten tumor suppressor gene in vivoM Groszer
Howard Hughes Medical Institute, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095, USA
Science 294:2186-9. 2001..However, cell fate commitments of the progenitors were largely undisturbed. Our results suggest that PTEN negatively regulates neural stem cell proliferation...
- YC-1, a novel potential anticancer agent, inhibit multidrug-resistant protein via cGMP-dependent pathwayChin Chuan Hung
Department of Pharmacy, College of Pharmacy, China Medical University, No 91 Hsueh Shih Road, Taichung, Taiwan, 40402, Republic of China
Invest New Drugs 29:1337-46. 2011..These results indicated that YC-1 inhibited Pgp efflux via the NO-cGMP-PKG-ERK signaling pathway through noncompetitive inhibition. The present study revealed that YC-1 could be a good candidate for development as a MDR modulator...
- Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractionsDeborah Pye
Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool L69 3GA, UK
J Physiol 581:309-18. 2007....
- Oxidative stress during aging of stationary cultures of the yeast Saccharomyces cerevisiaeW Jakubowski
Department of Molecular Biophysics, University of Lodz, Lodz, Poland
Free Radic Biol Med 28:659-64. 2000..The magnitude of this stress was augmented in antioxidant-deficient strains, devoid of superoxide dismutases and catalases, and of decreased glutathione content...
- In vitro p-glycoprotein inhibition assays for assessment of clinical drug interaction potential of new drug candidates: a recommendation for probe substratesJarkko Rautio
Preclinical Drug Metabolism and Pharmacokinetics, GlaxoSmithKline, Inc, Research Triangle Park, NC 27709, USA
Drug Metab Dispos 34:786-92. 2006..Finally, a strategy integrating results from in vitro assays (efflux, inhibition, and ATPase) is provided to further guide clinical interaction studies...
- Methods to measure the reactivity of peroxynitrite-derived oxidants toward reduced fluoresceins and rhodaminesPeter Wardman
University of Oxford, Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom
Methods Enzymol 441:261-82. 2008..This chapter outlines methods for estimation of key rate constants involving peroxynitrite-derived oxidants...
- In situ detection and measurement of intracellular reactive oxygen species in single isolated mature skeletal muscle fibers by real time fluorescence microscopyJesus Palomero
Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool, United Kingdom
Antioxid Redox Signal 10:1463-74. 2008..This technique has advantages over previous approaches because of the maturity of the fibers and the analysis of single cells, which prevent contributions from nonmuscle cells...
- Transcutaneous measurement of renal function in conscious miceAndrea Schreiber
Institute of Physiology, University of Regensburg, Regensburg, Germany
Am J Physiol Renal Physiol 303:F783-8. 2012..In summary, we describe a technique allowing the measurement of renal function in freely moving mice independent of blood or urine sampling as well as of laboratory assays...
- Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin IKurt W Marek
Department of Biochemistry, University of California, San Francisco, 94143, USA
Neuron 36:805-13. 2002..We demonstrate that Syt I is required for a post-docking step during vesicle fusion but does not function to stabilize the docked vesicle state...
- A division-dependent compartmental model for computing cell numbers in CFSE-based lymphocyte proliferation assaysH T Banks
Center for Research in Scientic Computation, Center for Quantitative Sciences in Biomedicine, North Carolina State University, Raleigh, NC 27695 8212, United States
Math Biosci Eng 9:699-736. 2012..Variability in cellular autofluorescence is found to play a significant role in the data, as well. Finally, the compartmental model is compared to previous work, and statistical aspects of the experimental data are discussed...
- Corneal alternations induced by topical application of benzalkonium chloride in rabbitWensheng Chen
Eye Institute and affiliated Xiamen Eye Center of Xiamen University, Fujian, China
PLoS ONE 6:e26103. 2011..A high concentration of BAC breaks down the barrier integrity of corneal endothelium, concomitant with the disruption of PAMR and remodeling of apical junctional complex in vivo...
- Synthesis of rhodamines from fluoresceins using Pd-catalyzed C-N cross-couplingJonathan B Grimm
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
Org Lett 13:6354-7. 2011....
- Evaluation of multitype mathematical models for CFSE-labeling experiment dataHongyu Miao
Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 630, Rochester, NY 14642, USA
Bull Math Biol 74:300-26. 2012..This model was then employed to understand the proliferation pattern of CD4+ and CD8+ T cells under polyclonal stimulation...
- Increased methylglyoxal and oxidative stress in hypertensive rat vascular smooth muscle cellsLingyun Wu
Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, Canada
Hypertension 39:809-14. 2002..Our findings suggest that that elevated methylglyoxal and associated oxidative stress possibly contribute to the pathogenesis of hypertension...
- Detection of reactive oxygen and reactive nitrogen species in skeletal muscleC L Murrant
Department of Pharmacology and Physiology, University of Rochester, Rochester, New York 14642, USA
Microsc Res Tech 55:236-48. 2001..Eventual delineation of the mechanisms whereby ROS and RNS regulate cellular function will hinge on our understanding of the production and distribution of ROS and RNS within skeletal muscle...
- Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: In vitro study with finite and infinite dosage applicationMing Chen
Department of Pharmaceutical Technology, Friedrich Schiller University Jena, Lessing str 8, D 07743 Jena, Germany
Int J Pharm 408:223-34. 2011..The results also revealed that the factors influencing the drug skin distribution concern the physicochemical characteristics of the drug, the choice of the vehicle formulation and the application mode applied...
- The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagyJ J Lemasters
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, CB No 7090, 236 Taylor Hall, Chapel Hill, NC 27799 7090, USA
Biochim Biophys Acta 1366:177-96. 1998..A model is proposed in which onset of the MPT to increasing numbers of mitochondria within a cell leads progressively to autophagy, apoptosis and necrotic cell death...
- Tuberization in potato involves a switch from apoplastic to symplastic phloem unloadingR Viola
Unit of Plant Biochemistry, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
Plant Cell 13:385-98. 2001..Evidence is presented that the apical and lateral tuber buds function as isolated domains with respect to sucrose unloading and metabolism...
- Continuous perfusion microfluidic cell culture array for high-throughput cell-based assaysPaul J Hung
Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California, 485 Evans Hall, Berkeley, CA 94720, USA
Biotechnol Bioeng 89:1-8. 2005..The cell culture array could offer a platform for a wide range of assays with applications in drug screening, bioinformatics, and quantitative cell biology...
- Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicalsMarta Wrona
Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex HA6 2JR, UK
Free Radic Biol Med 38:262-70. 2005..These data suggest that probe oxidation may often be in competition with reaction of the radicals with cellular antioxidants...
- Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series dataEdwin D Hawkins
Immunology Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia
Nat Protoc 2:2057-67. 2007..In this protocol, we present a detailed method for examining time-series data using graphical and computer-based procedures available to all experimenters...
- Evaluation of fluorescent dyes for measuring intracellular glutathione content in primary cultures of human neurons and neuroblastoma SH-SY5YJordi Sebastia
Department of Pharmacology and Toxicology, Institut d Investigacions Biomediques de Barcelona, CSIC, IDIBAPS, Barcelona, Spain
Cytometry A 51:16-25. 2003..To study GSH content in human neuronal cell cultures, thiol-sensitive fluorescent techniques requiring a small number of cells may be of great value, but their GSH specificity has not been established in these cells...
- New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyesBenjamin J C Quah
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia
J Immunol Methods 379:1-14. 2012....
- Estimation of cell proliferation dynamics using CFSE dataH T Banks
Center for Research in Scientific Computation, North Carolina State University, Raleigh, NC 27695 8212, USA
Bull Math Biol 73:116-50. 2011..The resulting new cell dynamics model should prove useful in proliferation assay tracking and modeling, with numerous applications in the biomedical sciences...
- Inhibition of HIV-1 Tat-dependent trans activation by steric block chimeric 2'-O-methyl/LNA oligoribonucleotidesA Arzumanov
Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, UK
Biochemistry 40:14645-54. 2001..An alternating PS-containing 3'-FAM OMe 12-mer oligonucleotide exhibited partial inhibition of trans-activation activity, but this was correlated with a similar effect on control gene expression, suggesting nonspecific inhibition...
- Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cellsS Urbani
Hematology Unit, Careggi Hospital, Viale Morgagni 85, 50134 Florence, Italy
Cytotherapy 8:243-53. 2006....
- Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceinsCarla C Spagnuolo
Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria Pabellon II, 1428, Buenos Aires, Argentina
J Am Chem Soc 128:12040-1. 2006..The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes...
- Identification and characterization of the canine multidrug resistance-associated proteinLiandong Ma
Division of Cancer Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
Mol Cancer Ther 1:1335-42. 2002..Knowledge of species differences between canine and human proteins will aid in the design of appropriate pharmacokinetic and toxicokinetic studies for the preclinical evaluation of MRP1 modulators...
- Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanismsMatthieu Marin
Laboratory of Ecotoxicology, UPRES EA 3222, IFRMP 23, University of Le Havre, BP 540 76058 Le Havre, France
Mar Environ Res 58:209-13. 2004..We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity...
- Identification of an orthogonal peptide binding motif for biarsenical multiuse affinity probesBaowei Chen
Cell Biology and Biochemistry Group, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
Bioconjug Chem 18:1259-65. 2007....
- Quantifying cell turnover using CFSE dataVitaly V Ganusov
Department of Biology, Emory University, Atlanta, GA 30322, USA
J Immunol Methods 298:183-200. 2005..We propose that measuring an additional parameter, the fraction of cells in division, may allow estimation of all model parameters including the death rates during different stages of the cell cycle...
- Fast laser-induced solute release from liposomes sensitized with photochromic lipid: effects of temperature, lipid host, and sensitizer concentrationR H Bisby
Science Research Institute, University of Salford, Salford, M5 4WT, United Kingdom
Biochem Biophys Res Commun 262:406-10. 1999..The results presented are relevant to the design of photostimulated drug delivery systems and to potential applications of photosensitive liposomes as caging agents for biological effectors...
- The physiological response of the marine platyhelminth Macrostomum lignano to different environmental oxygen concentrationsG A Rivera-Ingraham
Department of Functional Ecology, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
J Exp Biol 216:2741-51. 2013....
- Direct fluorometric analysis of a newly synthesised fluorescein-labelled marker for glomerular filtration rateJohannes Pill
Roche Diagnostics GmbH, Sandhofer Str 116, 68305, Mannheim, Germany
Anal Bioanal Chem 382:59-64. 2005..The dramatic decrease in analytical effort will result in a significantly higher acceptability of GFR determination...
- Flow cytometric analysis of chronic and acute toxicity of copper(II) on the marine dinoflagellate Amphidinium carteraeO M Lage
Departamento de Botânica da Faculdade de Ciências and Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Porto, Portugal
Cytometry 44:226-35. 2001....
- Multiple lipid compartments slow vesicle contents release in lipases and serumCecile Boyer
Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA
ACS Nano 1:176-82. 2007..The vesosome is a better mimic of eukaryotic cell structure and demonstrates the benefits of multiple internal bilayer-enclosed compartments...
- A general mathematical framework to model generation structure in a population of asynchronously dividing cellsKalet Leon
Centro de Inmunologia Molecular, Habana, Cuba
J Theor Biol 229:455-76. 2004....
- Visualization and enrichment of live putative cancer stem cell populations following p53 inactivation or Bax deletion using non-toxic fluorescent dyesJoshua E Allen
Laboratory of Molecular Oncology and Cell Cycle Regulation, Departments of Medicine Hematology Oncology, Genetics and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
Cancer Biol Ther 8:2194-205. 2009....
- Analysis and simulation of division- and label-structured population models : a new tool to analyze proliferation assaysJ Hasenauer
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum Munchen, Neuherberg, Germany
Bull Math Biol 74:2692-732. 2012..This is illustrated by modeling and simulating a proliferating population with division number-dependent proliferation rate...
- Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labelingXiao Qi Wang
The Central Laboratory, The First Teaching Hospital of Jilin University, Changchun 130021, China
Acta Biochim Biophys Sin (Shanghai) 37:379-85. 2005....
- Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl esterBen J C Quah
Division of Immunology and Genetics, John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory, Australia
Nat Protoc 2:2049-56. 2007..An important feature of the technique is that division-dependent changes in the expression of cell-surface markers and intracellular proteins are easily quantified by flow cytometry...
- A new model for the estimation of cell proliferation dynamics using CFSE dataH T Banks
Center for Research in Scientific Computation, North Carolina State University, Raleigh, NC 27695 8212, USA
J Immunol Methods 373:143-60. 2011..It is shown that these improvements result in a model with a strong physiological basis which is fully capable of replicating the behavior observed in the data...
- New method to quantify erythrophagocytosis by autologous monocytesRolf Fendel
Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
Cytometry A 71:258-64. 2007..In contrast, erythrophagocytosis by autologous phagocytes is more difficult to quantify...
- Fluorescence microplate assay for the detection of oxidative burst products in tobacco cell suspensions using 2',7'-dichlorofluoresceinIsak B Gerber
Department of Biochemistry, RAU University, Auckland Park, Johannesburg, South Africa
Methods Cell Sci 25:115-22. 2003....
- New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applicationsStephen R Adams
Department of Pharmacology, Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, and Biomedical Sciences Program, University of California, San Diego, La Jolla, California 92093 0647, USA
J Am Chem Soc 124:6063-76. 2002..Analogous biarsenicals enable affinity chromatography, fluorescence anisotropy measurements, and electron-microscopic localization of tetracysteine-tagged proteins...
- Up-regulation of multidrug resistance P-glycoprotein via nuclear factor-kappaB activation protects kidney proximal tubule cells from cadmium- and reactive oxygen species-induced apoptosisF Thevenod
Department of Physiology II, University of Saarland, 66421 Homburg, Germany
J Biol Chem 275:1887-96. 2000..These data suggest that mdr1 up-regulation, at least in part, provides anti-apoptotic protection for PT cells against cadmium-mediated stress...
- Interaction of cationic antimicrobial peptides with model membranesL Zhang
Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
J Biol Chem 276:35714-22. 2001..Overall, whereas all studied cationic antimicrobial peptides interacted with membranes, they were quite heterogeneous in their impact on these membranes...
- Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticlesCuiping Yao
Key Laboratory of Biomedical Information Engineering of Ministry of Education, China
J Biomed Opt 10:064012. 2005..Furthermore, a dependence on particle size is found for these interactions in the different cell lines. The results suggest that after optimization, this method could be used for gene transfection and gene therapy...
- Liposomes increase skin penetration of entrapped and non-entrapped hydrophilic substances into human skin: a skin penetration and confocal laser scanning microscopy studyD D Verma
Institut für Pharmazeutische Technologie und Biopharmazie, Philipps Universitat Marburg, Marburg, Germany
Eur J Pharm Biopharm 55:271-7. 2003..The results indicated that phospholipid vesicles not only carry the entrapped hydrophilic substance, but also the non-entrapped hydrophilic substance into the SC and possibly into the deeper layers of the skin...
- Keratoconjunctivitis sicca modifies epithelial stem cell proliferation kinetics in conjunctivaWensheng Chen
Tianjin Medical University Eye Center, Tianjin, China
Cornea 26:1101-6. 2007..The objective of this study was to examine the epithelial stem cell proliferation kinetics in a rat model with keratoconjunctivitis sicca (KCS)...
- Postnatal age influences hypoglycemia-induced neuronal injury in the rat brainKathleen Ennis
Division of Neonatology, Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA
Brain Res 1224:119-26. 2008..Varying the duration of fasting did not alter the severity of regional cell injury. These results suggest that postnatal age influences the regional vulnerability to hypoglycemia-induced neuronal death in the rat brain...
- Pumping of drugs by P-glycoprotein: a two-step process?Thomas Litman
Bioinformatics Centre, University of Copenhagen, Universitetsparken 15, Bldg 10, DK 2100 Copenhagen, Denmark
J Pharmacol Exp Ther 307:846-53. 2003..We suggest that the vinblastine/cyclosporin A/XR9576-binding site accepts daunorubicin at the cytoplasmic face and transfers it to the verapamil-binding site, from where daunorubicin is emptied at the extracellular surface...
- Evaluation and comparison of MRP1 activity with three fluorescent dyes and three modulators in leukemic cell linesA Lale Dogan
Laboratoire INSERM E03 55 EA 1529, Département d Onco Hématologie, Hôpital Hotel Dieu AP HP, Universite Paris 6, 1 Place du Parvis Notre Dame, Paris 75004, France
Leuk Res 28:619-22. 2004..In conclusion, calcein-AM and CFDA uptake assays are the best choices to probe MRP1 activity and combination of two modulators may improve the efficiency of these assays...
- Impaired renal secretion of substrates for the multidrug resistance protein 2 in mutant transport-deficient (TR-) ratsRosalinde Masereeuw
Department of Pharmacology and Toxicology, University Medical Center Nijmegen, Nijmegen Center for Molecular Life Sciences, Nijmegen, The Netherlands
J Am Soc Nephrol 14:2741-9. 2003..We conclude that the renal clearance of the Mrp2 substrates calcein and fluo-3 is significantly reduced in TR(-) rat; for LY, the absence of the transporter may be compensated for by (an)other organic anion transporter(s)...
- Loss or mismatch of MHC class I is sufficient to trigger NK cell-mediated rejection of resting lymphocytes in vivo - role of KARAP/DAP12-dependent and -independent pathwaysLinda Oberg
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
Eur J Immunol 34:1646-53. 2004..However, activation of the effector or the target enhanced rejection and shifted the balance between different signaling pathways involved...
- Fluorophotometric determination of hydrogen peroxide with fluorescin in the presence of cobalt (II) and reaction against other reactive oxygen speciesRyosuke Nakahara
Department of Clinical Chemistry, Osaka University of Pharmaceutical Sciences, 4 20 1 Nasahara, Takatsuki, Osaka 569 1094, Japan
J Fluoresc 19:769-75. 2009..and it was possible to detect them with a high sensitivity. In addition, this proposed method was applied to the recovery tests of H2O2 in calf serum, human saliva, rain water, and wheat noodles; the results were satisfactory...
- Distributed parameter identification for a label-structured cell population dynamics model using CFSE histogram time-series dataTatyana Luzyanina
Institute of Mathematical Problems in Biology, RAS, Pushchino, Russia
J Math Biol 59:581-603. 2009..We show that the solution of the regularized parameter estimation problem is consistent with the data set with an accuracy within the noise level in the measurements...
- Functional detection of MDR1/P170 and MRP/P190-mediated multidrug resistance in tumour cells by flow cytometryN Feller
Department of Medical Oncology, Free University Hospital, Amsterdam, The Netherlands
Br J Cancer 72:543-9. 1995..With these functional assays the role and relative importance of P-gp and MRP can be studied in, for example, haematological malignancies...
- Intracellular generation of reactive oxygen species by contracting skeletal muscle cellsFrancis McArdle
Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool L69 3GA, UK
Free Radic Biol Med 39:651-7. 2005....
- Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivativesAndreas Ebner
University of Linz, Linz, Austria
Methods Mol Biol 418:73-88. 2008..This competitive assay correctly measures the small dose of d-biotin in multivitamin tablets (e.g., 150 microg in 5 g of solid) after subtracting the background fluorescence of the colored aqueous solution...
- Functionality of drug efflux pumps in antimonial resistant Leishmania donovani field isolatesGoutam Mandal
Department of Pharmacology, Institute of Post Graduate Medical Education and Research, Kolkata, 244B Acharya JC Bose Road, Kolkata 700 020 India
Indian J Biochem Biophys 46:86-92. 2009..In conclusion, our data suggested that overfunctioning of a MRP-like pump contributed towards generation of Sb-R phenotype in L. donovani field isolates...
- Differential expression of sphingolipids in MRP1 overexpressing HT29 cellsJ W Kok
Department of Physiological Chemistry, Groningen Institute of Drug Studies, University of Groningen, Groningen, The Netherlands
Int J Cancer 87:172-8. 2000..In addition, ceramide, a lipid messenger molecule involved in apoptosis-related signal transduction processes, was much more abundant in HT29(col) cells, which is indicative of a stress response...
- Shell click-crosslinked (SCC) nanoparticles: a new methodology for synthesis and orthogonal functionalizationMaisie J Joralemon
Washington University in Saint Louis, Center for Materials Innovation and Department of Chemistry, Missouri 63130 4899, USA
J Am Chem Soc 127:16892-9. 2005....
- The oxidation of 2',7'-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy?Marcelo G Bonini
Laboratory of Pharmacology and Chemistry, Ntional Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
Free Radic Biol Med 40:968-75. 2006..Our findings demonstrate that this assay is subject to a serious artifact in that it produces what it is purported to measure; therefore, its use in biological systems should be approached with caution...
- A simple spectrophotometric streptavidin-biotin binding assay utilizing biotin-4-fluoresceinMark J Waner
Department of Chemistry, John Carroll University, 20700 North Park Blvd, University Heights, OH 44118 USA
J Biochem Biophys Methods 70:873-7. 2008....
- Statistical determination of threshold for cellular division in the CFSE-labeling assayDacheng Liu
Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, NY 14642, USA
J Immunol Methods 312:126-36. 2006..071% (95% confidence) and 0.114% (99% confidence) of total CFSE-labeled cells under our laboratory conditions. We offer our methods presented here for other investigators to calculate a threshold in their own CFSE-labeling experiments...
- Flow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cellsM Graça da Silveira
Instituto de Tecnologia Quimica e Biologica, 2780 Oeiras, Portugal
Appl Environ Microbiol 68:6087-93. 2002..This information is of major importance in studies of microbial fermentations in order to assign bulk activities measured by classical methods to the very active cells that are effectively responsible for the observations...
- Endogenous and endobiotic induced reactive oxygen species formation by isolated hepatocytesArno G Siraki
Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, 19 Russell Street, Toronto, Ontario, Canada M5S 2S2
Free Radic Biol Med 32:2-10. 2002..Hepatocyte catalyzed dichlorofluorescin oxidation induced by oxidase substrates, e.g., benzylamine, was correlated with the cytotoxicity induced in catalase-inhibited hepatocytes...
- Measurement of diffusion within the cell wall in living roots of Arabidopsis thalianaEric M Kramer
Biology Department, University of Massachusetts, 611 N Pleasant St, Amherst, MA 01003, USA
J Exp Bot 58:3005-15. 2007..These measurements constrain models of solute transport, and are important for quantitative models of hormone signalling during plant growth and development...
- Caged probes: a novel tool in studying symplasmic transport in plant tissuesH J Martens
Department of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
Protoplasma 223:63-6. 2004..Transport via the symplasmic pathway was challenged by plasmolysing the tissue. The experiments confirmed the symplasmic nature of tracer transport...
- Quantitative tracking of Cryptosporidium infection in cell culture with CFSEHanping Feng
Division of Infectious Diseases, Tufts University, Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
J Parasitol 92:1350-4. 2006..Unlike immunofluorescent assays, CFSE labeling permitted the tracking of the initial invasion of C. parvum. Such an assay may be useful for studying the dynamics of host cell-parasite interaction and possibly for drug screening...
- Heterogeneous cell response to topotecan in a CFSE-based proliferation testGiada Matera
Biophysics Unit, Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy
Cytometry A 62:118-28. 2004..However, intercell variability of CFSE loading results in overlapping peaks, thereby limiting its use with cancer cell lines...
- Self-assembly of recombinant amphiphilic oligopeptides into vesiclesAlbert J van Hell
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands
Biomacromolecules 8:2753-61. 2007..In addition, it was demonstrated that water-soluble molecules can be entrapped inside these peptide vesicles. Such peptide vesicles may find applications as biodegradable drug delivery systems with a pH-dependent release profile...
- Computational analysis of CFSE proliferation assayTatyana Luzyanina
Institute of Mathematical Problems in Biology, RAS, Pushchino, Russia
J Math Biol 54:57-89. 2007..Overall, our study suggests that the heterogeneity inherent in cell kinetics should be explicitly incorporated into the structure of mathematical models...
- Use of CFSE staining of borreliae in studies on the interaction between borreliae and human neutrophilsHelena Tuominen-Gustafsson
Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, FI 20520 Turku, Finland
BMC Microbiol 6:92. 2006..Carboxyfluorescein diacetate, succinimidyl ester (CFSE) labelling has been previously used for studying the adhesion of labelled bacteria to host cells and the uptake of labelled substrates by various cells using flow cytometry...
- Eosin B as a novel antimalarial agent for drug-resistant Plasmodium falciparumKristen M Massimine
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA
Antimicrob Agents Chemother 50:3132-41. 2006..In addition, preliminary studies suggest that eosin B is also acting as a redox cycling compound. Overall, our data suggest that eosin B is an effective lead compound for the development of new, more effective antimalarial drugs...
- Estimating lymphocyte division and death rates from CFSE dataRob J de Boer
Theoretical Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
Bull Math Biol 68:1011-31. 2006..J. Immunol. 170:4963-4972, 2003] model into the classical smith-martin model, and into a model with arbitrary probability distributions for death and division through subsequent divisions...
- Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE)Becca Asquith
Department of Immunology, Imperial College London, Norfolk Place, London W2 1PG, UK
Proc Biol Sci 273:1165-71. 2006..We illustrate this approach using in vivo CFSE labelling of B lymphocytes in sheep...
- pH fluorescent probes: chlorinated fluoresceinsFeng Yan Ge
College of Chemistry and Chemical Engineering, Donghua University, Shanghai, People s Republic of China
J Fluoresc 18:741-7. 2008A series of regiospecific chlorinated fluoresceins have been synthesized by the reaction of the regiospecific chlorinated resorcinols with chlorinated phthalic anhydride. The regioisomers were successfully separated by chromatography...
- Reconstruction of cell population dynamics using CFSEAndrew Yates
Department of Biology, Emory University, Atlanta, GA 30322, USA
BMC Bioinformatics 8:196. 2007..This gives rise to heterogeneity in proliferating cell populations. Branching processes provide a natural means of describing this behaviour...
- Endothelin B receptor-mediated regulation of ATP-driven drug secretion in renal proximal tubuleR Masereeuw
Department of Pharmacology, University of Nijmegen, Nijmegen, The Netherlands
Mol Pharmacol 57:59-67. 2000..These are the first results linking ET to the control of xenobiotic transport and the first demonstrating control of renal multidrug resistance-associated protein 2 and P-glycoprotein by a hormone...
- A comparative study of carboxyfluorescein diacetate and carboxyfluorescein diacetate succinimidyl ester as indicators of bacterial activityDaniel Hoefel
The Cooperative Research Centre for Water Quality and Treatment, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia
J Microbiol Methods 52:379-88. 2003..This study shows that CFDA/SE is a poor marker of bacterial activity...
- Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl esterA B Lyons
Discipline of Pathology, The University of Tasmania, Hobart, Tasmania, Australia
Immunol Cell Biol 77:509-15. 1999..Furthermore, viable cells from defined generation numbers can be sorted by flow cytometry for functional analysis...
- A novel flow cytometric assay for quantitating adherence of Helicobacter pylori to gastric epithelial cellsR P Logan
Division of Gastroenterology, University Hospital, Nottingham, UK
J Immunol Methods 213:19-30. 1998..Fluorescent labelling of H. pylori with CFDA-SE in combination with flow cytometry provides a quick, specific, and sensitive method to quantitate in vitro the adherence of H. pylori...
- Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilutionA B Lyons
Discipline of Pathology, Faculty of Health Science, The University of Tasmania, GPO Box 252 29, 7001, Hobart, TAS, Australia
J Immunol Methods 243:147-54. 2000..The CFSE technique can be used to determine kinetics of immune responses, track proliferation in minor subsets of cells and follow the acquisition of differentiation markers or internal proteins linked to cell division...
- Fluorescent dyes for lymphocyte migration and proliferation studiesC R Parish
Division of Immunology and Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia
Immunol Cell Biol 77:499-508. 1999..In the future it is highly likely that additional fluorescent dyes, with different spectral properties to CFSE, will become available, as well as membrane inserting fluorescent dyes that more homogeneously label lymphocytes than PKH26...
- Noninvasive in vivo fluorescence measurement of airway-surface liquid depth, salt concentration, and pHS Jayaraman
Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521, USA
J Clin Invest 107:317-24. 2001..78 +/- 0.2. These results establish by a noninvasive approach the key properties of the ASL and provide direct evidence that the ASL is approximately isotonic and not saltier in cystic fibrosis...
- beta-lactamase as a marker for gene expression in live zebrafish embryosE Raz
Institute for Biology 1, University of Freiburg, Hauptstrasse 1, Freiburg, D 79104, Germany
Dev Biol 203:290-4. 1998..We suggest that this new reporter system provides a major advancement in sensitivity over the existing methods for monitoring gene expression in vivo during early embryogenesis...
- A molecular docking strategy identifies Eosin B as a non-active site inhibitor of protozoal bifunctional thymidylate synthase-dihydrofolate reductaseChloe E Atreya
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 278:14092-100. 2003..Additionally, eosin B was found to be a 180 microm inhibitor of Toxoplasma gondii in both biochemical and cell culture assays...
- Fluorescent sensors for Zn(2+) based on a fluorescein platform: synthesis, properties and intracellular distributionS C Burdette
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA
J Am Chem Soc 123:7831-41. 2001..The X-ray crystal structure of a 2:1 Zn(2+):Zinpyr-1 complex has also been solved, and is the first structurally characterized example of a complex of fluorescein substituted with metal binding ligands...
- Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate readerH Wang
Neuroscience Laboratory, USDA ARS, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA
Free Radic Biol Med 27:612-6. 1999..This method is reliable and efficient for evaluating the potency of pro-oxidants and can be used to evaluate the efficacy of antioxidants against OS in cells...
- Comparison of in vitro P-glycoprotein screening assays: recommendations for their use in drug discoveryDietmar Schwab
F Hoffmann LaRoche Ltd, Pharmaceuticals Division, CH 4070 Basel, Switzerland
J Med Chem 46:1716-25. 2003..Recommendations are given on screening strategies which can be applied to different phases of the drug discovery and development process...
- Analysis of cell kinetics using a cell division marker: mathematical modeling of experimental dataSamuel Bernard
Département de mathématiques et de statistique and Centre de recherches mathématiques, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
Biophys J 84:3414-24. 2003..Our results indicate that after three days in vitro, primitive murine bone marrow cells have parameters beta = 2.2 day(-1), tau = 0.3 day, gamma = 0.3 day(-1), and micro = 0.05 day(-1)...
- Design of a practical fluorescent probe for superoxide based on protection-deprotection chemistry of fluoresceins with benzenesulfonyl protecting groupsHatsuo Maeda
Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamada oka, Suita, Osaka 565 0871, Japan
Chemistry 13:1946-54. 2007A strategy for designing probes based on protection-deprotection chemistry involving fluoresceins and their benzenesulfonyl (BES) derivatives has led to the development of a much more practical superoxide (O(2) (-...
- Polyamine deficiency leads to accumulation of reactive oxygen species in a spe2Delta mutant of Saccharomyces cerevisiaeManas K Chattopadhyay
Laboratory of Biochemistry and Genetics, Building 8, Room 223, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Yeast 23:751-61. 2006..Polyamine-deficient cells incubated anaerobically show markedly less cell death. These data show that part of the function of polyamines is protection of the cells from accumulation of ROS...
- Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1Rachel A Egler
Department of Pediatrics, Section of Hematology Oncology, Children s Hospital of Pittsburgh, Rangos Research Center, 3460 Fifth Ave, USA
Oncogene 24:8038-50. 2005..prdx1-/- mice should be useful in studying the role of oxidative DNA damage in the causation of cancer and its prevention by antioxidants. They should also help in studying the relationship between oncogenes such as c-Myc and DNA damage...
- A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicityH Lecoeur
, URA CNRS 1930, , Institut Pasteur, 28 rue du Dr. Roux, 75724 Cedex 15, Paris, France
J Immunol Methods 253:177-87. 2001..This new assay should contribute to a better understanding of the mechanisms involved in cell-mediated cytotoxicity in normal and pathological situations...
- Novel Extended Red-Shifted Rhodamine DyesNICHOLAS CAIRNS; Fiscal Year: 2004..In existing applications of commonly used short and medium wavelength dyes such as coumarins, fluoresceins and rhodamines, assay or tissue components can severely interfere with the fluorescence properties of tracers ..
- DEVELOPMENT OF CHLORIDE-SENSITIVE FLUORESCENT INDICATORSAlan Verkman; Fiscal Year: 1991..Based on the utility of fluorescence probes in examining physiological questions (fluoresceins to study pH, fura-2 to study Ca), we have been developing new fluorescence methods to study Cl transport in ..
- Modeling, Estimation and Control in HIV DynamicsH Banks; Fiscal Year: 2005....
- In Vivo Gene Silencing with siRNA After Brain TraumaHELEN HELLMICH; Fiscal Year: 2006..unreadable] [unreadable]..
- Mineral and Mechanical Properties of BoneSarandeep Huja; Fiscal Year: 2005..Future interventional studies will examine the mechanisms for the initial and the long-term adaptive response in material properties of bone. ..
- GENE EXPRESSION-ENVIRONMENT LINK IN CEREBRAL ISCHEMIATERESITA BRIONES; Fiscal Year: 2006..Additionally, the sensory motor deficit seen following ischemic damage can affect the long-term outcome of these survivors. ..
- A THERAPEUTIC MODEL FOR CEREBRAL ISCHEMIATERESITA BRIONES; Fiscal Year: 2003..From a nursing perspective, results from this study are important clinically because the needs of most survivors of brain injury often times are for continuous nursing, while use of medical services may be intermittent. ..
- Antioxidant Functions of Lipoic AcidJames May; Fiscal Year: 2004..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
- MOLECULAR ANGLE TRANSITIONS IN MUSCLETHOMAS BURGHARDT; Fiscal Year: 2001....
- Antioxidant vitamins in models of Alzheimer's DiseaseJames May; Fiscal Year: 2008..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
- Antioxidant Interaction of Selenium and Vitamins C & EJames May; Fiscal Year: 2008..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
- AGE/NUTRITION/GENES IN MODELS OF PSYCHIATRIC DISORDERSGary Gibson; Fiscal Year: 2007..An understanding of these mechanisms will likely suggest new ways to overcome the consequences of the mild, chronic impairment of oxidative metabolism that accompanies numerous age-related neurodegenerative disorders. ..
- Aging as a risk factor for seizure-induced cell deathPAULA SCHAUWECKER; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable]..
- Multiphoton microscopy using near infrared DyesSamuel Achilefu; Fiscal Year: 2006..Translation of the method and instrument developed from small animals to imaging of human Gl and cervical cancers is expected at the completion of the study. ..
- Synchrotron-Based Infrared Microscope for Chemical Imaging of DiseaseLisa Miller; Fiscal Year: 2007..This instrument will be unique worldwide. [unreadable] [unreadable] [unreadable]..
- Metal-Enhanced Fluorescence SensingJoseph Lakowicz; Fiscal Year: 2006..b. Develop RET immunoassays for PSA (or ACT-PSA) with the capture and detection antibodies. [unreadable] [unreadable]..
- A Chaperonin/Osmolyte Protein Folding Screen - STTR Phase IMark Fisher; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Peroxide, NO and Iron Signaling in Endothelial DamageBalaraman Kalyanaraman; Fiscal Year: 2007..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
- Metal Carcinogenesis: New ConceptsAnatoly Zhitkovich; Fiscal Year: 2006..unreadable] [unreadable] [unreadable] [unreadable]..
- MICELLAR CARRIERS FOR SPARINGLY SOLUBLE PHARMACEUTICALSVladimir Torchilin; Fiscal Year: 2007..unreadable] [unreadable]..
- ADVANCES IN OPTICS FOR BIOTECHNOLOGY, MEDICINE AND SURGERY CONFERENCESamuel Achilefu; Fiscal Year: 2007..unreadable] Aim 4: To promote interaction between the invited scientists and participating students, and to enable [unreadable] collaborations. [unreadable] [unreadable] [unreadable]..
- MULTIMODAL DOT-PET MOLECULAR PROBES AND TANDEM SYSTEMJoseph P Culver; Fiscal Year: 2010..abstract_text> ..
- Polypeptide Conformation and Interaction with Hsp70Silvia Cavagnero; Fiscal Year: 2009..abstract_text> ..
- Elevated potassium ion in lacrimal fluid and the health of the ocular surfaceJohn Ubels; Fiscal Year: 2009..If the high potassium in tears is important for the health of the ocular surface, this information will be useful in the development of improved treatments for dry eye disease. ..
- PHYSICOCHEMICAL INVESTIGATION OF TASTEVijay Lyall; Fiscal Year: 2010..The research will reveal how the sour taste defense mechanism functions to prevent the ingestion of acids. ..
- Chemical Approaches for the Discovery of New Cancer Therapeutic TargetsKazunori Koide; Fiscal Year: 2009....
- Long-Circulating Polymer-Modified LiposomesVladimir Torchilin; Fiscal Year: 2008..abstract_text> ..
- Sensitivity Mechanisms in Chromium ToxicityAnatoly Zhitkovich; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Genetics of neuromuscular synaptogenesis in zebrafishRita Balice Gordon; Fiscal Year: 2008..Identification of the underlying genetic defects in these mutants will expand our understanding of the molecular mechanisms that mediate the formation and function of neuromuscular and other synapses. ..
- Novel Neuroprotectants for TBIEdward Hall; Fiscal Year: 2008..Thousands experience long lasting brain injury that interferes with their ability to lead productive and full lives. We plan to test a promising new class of therapeutics for traumatic brain injury. [unreadable] [unreadable] [unreadable]..
- Renal medullary COX-2 in blood pressure controlTianxin Yang; Fiscal Year: 2008..The information resulted from these studies will provide new insight into the molecular mechanism responsible for long-term control of arterial blood pressure. ..
- Interaction of COX-2 AND nNOS in renin secretion (R01)Tianxin Yang; Fiscal Year: 2008..abstract_text> ..
- Image-guided Ultrasound-triggered Tumor TherapyAlexander Klibanov; Fiscal Year: 2007..Monitor blood flow within the tumor after treatment. Perform final optimization of the drug carrier formulation and ultrasound activation parameters. ..
- Chimeric Proteins to Enhance Crystal Formation of G Protein Coupled ReceptorsBrian Kobilka; Fiscal Year: 2006..Drugs acting on GPCRs can have an impact on a broad spectrum of diseases including: cardiovascular disease, pulmonary disease, inflammation, diabetes and obesity, behavioral disorders and Alzheimer's disease. ..
- HLA-DQ-derived RTLs for Treatment of Celiac DiseaseGREGORY BURROWS; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINESBalaraman Kalyanaraman; Fiscal Year: 2006..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
- Effects of Antioxidants on Human Macular PigmentsKyung Jin Yeum; Fiscal Year: 2006..unreadable] [unreadable]..
- CALCIUM DEPENDENT MEMBRANE BINDING PROTEINSCARL CREUTZ; Fiscal Year: 2002....
- HYPOGLYCEMIA-ASSOCIATED AUTONOMIC FAILURE AND THE BRAINNancy Tkacs; Fiscal Year: 2003..The research plan, candidate, and environment are well-suited for an ongoing career in fundamental diabetes research. ..
- SIGNAL TRANSDUCTION SYSTEMS IN ALZHEIMERS DISEASEGary Gibson; Fiscal Year: 2003..abstract_text> ..
- THE ROLE OF UROKINASE IN LUNG T LYMPHOCYTE RESPONSESMARGARET GYETKO; Fiscal Year: 2003..Determine if recombinant uPA can reverse the functional abnormalities in uPA minus/minus lymphocytes. ..
- K + EFFLUX--ROLE IN CA++ HOMEOSTASIS AND EXCITOTOXICITYLech Kiedrowski; Fiscal Year: 2003..These studies may yield a new target for pharmacological intervention to decrease neuronal death following ischemia or hypoglycemia, namely, to prevent excessive K+ loss from the brain during reperfusion. ..
- SPECIFICATION OF FAST AND SLOW MUSCLES IN ZEBRAFISHShao Jun Du; Fiscal Year: 2003..The long-term goal of this study is to elucidate the molecular mechanisms behind determination of muscle-specific cell fate during embryonic development. ..
- MECHANISM FOR BONE SIALOPROTEIN TO NUCLEATE BIOAPATITERONALD MIDURA; Fiscal Year: 2002..abstract_text> ..
- Dietary Cysteine:Conceptal Response to Oxidative StressCraig Harris; Fiscal Year: 2002....
- ROLE OF NITRIC OXIDE IN FERTILIZATIONDavid Epel; Fiscal Year: 2002..The possible role of NO in regulating the egg's ion channels (as part of polyspermy block) and sperm physiology (such as motility and the acrosome reaction) will also be investigated. ..
- FATTY ACID METABOLISM IN CARDIAC ISCHEMIADIANE HICKSON BICK; Fiscal Year: 2002..Location of a malonyl-CoA compartment that is inaccessible to CPT-I will shed light on the inconsistency between the high malonyl CoA content in heart and reliance on beta-oxidation. ..
- A HIGH PRESSURE FREEZER FOR ELECTRON MICROSCOPY USESGINA SOSINSKY; Fiscal Year: 2002..In all of these research projects, the primary goal is to understand the three-dimensional structure of organized cellular components and relate this morphology to their functionality...
- A Freeze-Fracture & Etching System for Northwestern UnivMark Johnson; Fiscal Year: 2002..abstract_text> ..
- NF-KB SIGNAL TRANSDUCTION IN BRAIN INJURYKEITH PENNYPACKER; Fiscal Year: 2003..These studies will define the role of NF-kB in the process of brain injury and subsequent repair and regeneration processes. ..
- Glial Maintenance of Local K+ and NeurotransmissionJULIAN MEEKS; Fiscal Year: 2005..These studies will determine the functional relevance of KIRs in hippocampal astrocytes and provide new insight into the role of KIRs in passive [K+]o buffering. ..
- CENTER FOR FLUORESCENCE SPECTROSCOPYJoseph Lakowicz; Fiscal Year: 2006..Abstract Not Provided. ..
- Genetic approaches to neurotrophin signalingRita Balice Gordon; Fiscal Year: 2006....
- Biophysical Analysis of Opioid Receptor StructureBrian Kobilka; Fiscal Year: 2005..Successful completion of these aims will provide the preliminary data for a more comprehensive study of opioid receptor structure and conformational dynamics in a Stage II CEBRA project. ..
- Peroxynitrite-Induced Oxidative Damage in TBIEdward Hall; Fiscal Year: 2007..abstract_text> ..
- Symposium on Imaging Agents and Molecular BeaconsSamuel Achilefu; Fiscal Year: 2004..Five sessions will be held and will cover topics on nuclear, magnetic resonance, ultrasound, radiographic, optical and miscellaneous imaging agents for medical applications. ..
- ANTIBODY-TARGETED POLYMERIC SYSTEMS FOR TUMOR IMAGINGVladimir Torchilin; Fiscal Year: 2006..abstract_text> ..
- Meeting:Society for Integrative and Comparative BiologyNeil Blackstone; Fiscal Year: 2004..Ultimately, this work will allow the power of genomics to be fully realized in biology and medicine. ..
- ANNEXIN PHOSPHORYLATION: CAUSES AND CONSEQUENCESCARL CREUTZ; Fiscal Year: 2004....
- MOLECULAR BIOLOGY OF INTERCELLULAR JUNCTIONSDaniel Goodenough; Fiscal Year: 2006..If these connexins are expressed in retinal neurons, mouse lines with targeted gene knockouts of these connexins will be prepared and studied for functional consequences. ..
- ROLE OF SPHINGOMYELIN IN LIPOPROTEIN METABOLISMPapasani Subbaiah; Fiscal Year: 2008..These studies will provide novel insights into the physiological role of this important phospholipid, and could possibly open up novel therapeutic targets against inflammation and atherosclerosis. ..
- Comparative In Vivo Biodistribution of Characterized Manufactured NanomaterialsNancy A Monteiro Riviere; Fiscal Year: 2010..These coordinated studies will provide data to link physical chemical characterizations to in vivo MNM disposition. ..
- LCAT AND PATHOPHYSIOLOGY OF PHOSPHOLIPID SPECIESPapasani Subbaiah; Fiscal Year: 2002..These studies should provide novel insights into the physiological importance of LCAT beyond its role in cholesterol esterification. ..
- BIACORE 3000 BiosensorRobert Bloch; Fiscal Year: 2002..We anticipate that this facility will provide its primary users, and ultimately others at the SoM and in the Baltimore area, with state-of-the-art biosensor technology. ..
- A Shared Multiparameter Imaging CytometerPaul Wallace; Fiscal Year: 2006..All the projects described in this application are relevant to the treatment of cancer and obtaining a better understanding of its biology. [unreadable] [unreadable] [unreadable]..
- INDICES OF BIOTIN NUTRITIONDonald Mock; Fiscal Year: 2001..4) Whether biotin deficiency of similar severity to that observed either early or late in human gestation causes increased rates of fetal malformation in the mouse. 5) The enzyme(s) responsible for biotin beta-oxidation. ..
- The Sarcolemma in FSHD and in the myd MouseRobert Bloch; Fiscal Year: 2003..We therefore anticipate making significant progress in understanding the cell biological changes that occur in FSHD skeletal muscle, and in determining which, if any, of these changes are related to the pathophysiology of FSHD. ..
- Obscurin & Myofibrils in Cardiac & Skeletal MuscleRobert Bloch; Fiscal Year: 2009..The results of our experiments should reveal some of the key mechanisms involved in assembling contractile proteins into sarcomeres and in determining their association with the SR. ..