spectinomycin

Summary

Summary: An antibiotic produced by Streptomyces spectabilis. It is active against gram-negative bacteria and used for the treatment of gonorrhea.

Top Publications

  1. pmc Novel spectinomycin/streptomycin resistance gene, aadA14, from Pasteurella multocida
    Corinna Kehrenberg
    Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft FAL, Höltystr 10, 31535 Neustadt Mariensee, Germany
    Antimicrob Agents Chemother 49:3046-9. 2005
  2. doi Simultaneous determination of lincomycin and spectinomycin residues in animal tissues by gas chromatography-nitrogen phosphorus detection and gas chromatography-mass spectrometry with accelerated solvent extraction
    Y Tao
    National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess 28:145-54. 2011
  3. ncbi HPLC determination of residues of spectinomycin in various tissue types from husbandry animals
    A A Bergwerff
    Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    Analyst 123:2139-44. 1998
  4. ncbi Detection of a novel aph(2") allele (aph[2"]-Ie) conferring high-level gentamicin resistance and a spectinomycin resistance gene ant(9)-Ia (aad 9) in clinical isolates of enterococci
    Mohammed Mahbub Alam
    Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060 8556, Japan
    Microb Drug Resist 11:239-47. 2005
  5. doi Development and validation of liquid chromatography tandem mass spectrometry methods for the determination of gentamicin, lincomycin, and spectinomycin in the presence of their impurities in pharmaceutical formulations
    K Vucićević-Prcetić
    DSP Chromatography, Bul Zorana Đinđića 166, Belgrade, Serbia
    J Pharm Biomed Anal 56:736-42. 2011
  6. ncbi Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3") (9)
    E Murphy
    Mol Gen Genet 200:33-9. 1985
  7. pmc Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi
    Daniel Criswell
    Division of Biological Sciences, The University of Montana, Missoula, MT 59812 4824, USA
    Antimicrob Agents Chemother 50:445-52. 2006
  8. ncbi Analysis of a formulation containing lincomycin and spectinomycin by liquid chromatography with pulsed electrochemical detection
    J Szúnyog
    Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, E Van Evenstraat 4, B 3000 Leuven, Belgium
    J Pharm Biomed Anal 29:213-20. 2002
  9. ncbi Development of a hydrophilic interaction chromatography-mass spectrometry assay for spectinomycin and lincomycin in liquid hog manure supernatant and run-off from cropland
    Kerry M Peru
    National Water Research Institute, Environment Canada, 11 Innovation Boulevard, Saskatoon, Sask, Canada S7N 3H5
    J Chromatogr A 1107:152-8. 2006
  10. doi A generic method for the quantitative analysis of aminoglycosides (and spectinomycin) in animal tissue using methylated internal standards and liquid chromatography tandem mass spectrometry
    Frederique L van Holthoon
    RIKILT Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD, P O Box 230, 6700 AE Wageningen, The Netherlands
    Anal Chim Acta 637:135-43. 2009

Detail Information

Publications186 found, 100 shown here

  1. pmc Novel spectinomycin/streptomycin resistance gene, aadA14, from Pasteurella multocida
    Corinna Kehrenberg
    Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft FAL, Höltystr 10, 31535 Neustadt Mariensee, Germany
    Antimicrob Agents Chemother 49:3046-9. 2005
    A novel spectinomycin/streptomycin resistance gene, designated aadA14, was detected on the mobilizable 5,198-bp plasmid pCCK647 from Pasteurella multocida. The aadA14 gene encodes an aminoglycoside adenylyltransferase of 261 amino acids...
  2. doi Simultaneous determination of lincomycin and spectinomycin residues in animal tissues by gas chromatography-nitrogen phosphorus detection and gas chromatography-mass spectrometry with accelerated solvent extraction
    Y Tao
    National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan 430070, China
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess 28:145-54. 2011
    ..spectrometry (GC-MS) was developed for qualitative and quantitative measurement of lincomycin and spectinomycin residues in food animal tissues...
  3. ncbi HPLC determination of residues of spectinomycin in various tissue types from husbandry animals
    A A Bergwerff
    Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    Analyst 123:2139-44. 1998
    An HPLC method was developed for the determination of bacteriostatic aminocyclitol spectinomycin (SP) in animal tissue products...
  4. ncbi Detection of a novel aph(2") allele (aph[2"]-Ie) conferring high-level gentamicin resistance and a spectinomycin resistance gene ant(9)-Ia (aad 9) in clinical isolates of enterococci
    Mohammed Mahbub Alam
    Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060 8556, Japan
    Microb Drug Resist 11:239-47. 2005
    ..ant(9)-Ia had been reported only in Staphylococcus aureus and encodes spectinomycin adenylyltransferase ANT(9)-I, which confers resistance to spectinomycin...
  5. doi Development and validation of liquid chromatography tandem mass spectrometry methods for the determination of gentamicin, lincomycin, and spectinomycin in the presence of their impurities in pharmaceutical formulations
    K Vucićević-Prcetić
    DSP Chromatography, Bul Zorana Đinđića 166, Belgrade, Serbia
    J Pharm Biomed Anal 56:736-42. 2011
    ..with tandem mass spectrometry (LC/MS/MS) methods for the determination of gentamicin, lincomycin and spectinomycin in the presence of their impurities were developed and tested...
  6. ncbi Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3") (9)
    E Murphy
    Mol Gen Genet 200:33-9. 1985
    ..This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin...
  7. pmc Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi
    Daniel Criswell
    Division of Biological Sciences, The University of Montana, Missoula, MT 59812 4824, USA
    Antimicrob Agents Chemother 50:445-52. 2006
    ..and characterized in vitro mutants of the Lyme disease agent Borrelia burgdorferi that are resistant to spectinomycin, kanamycin, gentamicin, or streptomycin, antibiotics that target the small subunit of the ribosome...
  8. ncbi Analysis of a formulation containing lincomycin and spectinomycin by liquid chromatography with pulsed electrochemical detection
    J Szúnyog
    Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, E Van Evenstraat 4, B 3000 Leuven, Belgium
    J Pharm Biomed Anal 29:213-20. 2002
    ..It allows the separation of a mixture of spectinomycin sulfate, lincomycin hydrochloride and their related substances...
  9. ncbi Development of a hydrophilic interaction chromatography-mass spectrometry assay for spectinomycin and lincomycin in liquid hog manure supernatant and run-off from cropland
    Kerry M Peru
    National Water Research Institute, Environment Canada, 11 Innovation Boulevard, Saskatoon, Sask, Canada S7N 3H5
    J Chromatogr A 1107:152-8. 2006
    A specific and sensitive analytical method was developed to extract and quantify spectinomycin and lincomycin in liquid hog manure supernatant and simulated rainfall run-off from manure-treated cropland...
  10. doi A generic method for the quantitative analysis of aminoglycosides (and spectinomycin) in animal tissue using methylated internal standards and liquid chromatography tandem mass spectrometry
    Frederique L van Holthoon
    RIKILT Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD, P O Box 230, 6700 AE Wageningen, The Netherlands
    Anal Chim Acta 637:135-43. 2009
    ..for the quantitative determination and confirmation of seven aminoglycosides (and relevant isomers) and spectinomycin in animal tissues...
  11. ncbi Efficacy of spectinomycin against Mycoplasma bovis induced pneumonia in conventionally reared calves
    F Poumarat
    Agence Francaise de Securite Sanitaire des Aliments, Site de Lyon, 31 av Tony Garnier, F 69364 Cedex 07, Lyon, France
    Vet Microbiol 80:23-35. 2001
    ..On the 6th day, spectinomycin 20mg/kg was given intramuscularly in three repeated doses at 24h intervals to six randomly chosen calves...
  12. pmc In vivo selection of spectinomycin-binding RNAs
    Jeff M Zimmerman
    Department of Biochemistry and Molecular Biology, 200 First Street SW, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Nucleic Acids Res 30:5425-35. 2002
    ..present case, we report short RNA transcripts whose expression confers bacterial resistance to the antibiotic spectinomycin. We provide evidence that such RNAs cause drug resistance by direct antibiotic binding, demonstrating a class ..
  13. pmc New gene cassettes for trimethoprim resistance, dfr13, and Streptomycin-spectinomycin resistance, aadA4, inserted on a class 1 integron
    P V Adrian
    Department of Medical Microbiology, University of the Witwatersrand, and the South African Institute for Medical Research, Johannesburg, South Africa
    Antimicrob Agents Chemother 44:355-61. 2000
    ..a new cassette, an aminoglycoside resistance gene of the class AADA ¿ANT(3")(9)-I, which encodes streptomycin-spectinomycin resistance, was identified...
  14. pmc First case of spectinomycin resistant Neisseria gonorrhoeae isolate in New Delhi, India
    M Bala
    Sex Transm Infect 81:186-7. 2005
  15. pmc Fitness cost due to mutations in the 16S rRNA associated with spectinomycin resistance in Chlamydia psittaci 6BC
    Rachel Binet
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814 4799, USA
    Antimicrob Agents Chemother 49:4455-64. 2005
    ..we studied the growth of four genetically defined spectinomycin-resistant (Spc(r)) clonal variants of Chlamydia psittaci 6BC isolated in the plaque assay...
  16. ncbi In vitro susceptibilities of field isolates of Mycoplasma agalactiae to oxytetracycline, tylosin, enrofloxacin, spiramycin and lincomycin-spectinomycin
    G R Loria
    Institute Zooprofilattico, Sperimentale della Sicilia, Via Rocco Dicillo, 4 I 90129, Palermo, Sicily, Italy
    Res Vet Sci 75:3-7. 2003
    ..The minimum inhibitory concentrations (MICs) of tetracycline, enrofloxacin, tylosin, spiramycin and a lincomycin:spectinomycin 1:2 combination, against 24 Sicilian isolates of Mycoplasma agalactiae, the causative organism of contagious ..
  17. ncbi Single-dose treatment of male patients with gonococcal urethritis using 2g spectinomycin: microbiological and clinical evaluations
    Munekado Kojima
    Department of Urology, Nagoya Urology Hospital, 5 34 Matsuba, Nagoya, Japan
    Int J Antimicrob Agents 32:50-4. 2008
    The microbiological and clinical efficacies of a single-dose treatment of 2g spectinomycin administered by intramuscular injection were studied in 365 male patients with gonococcal urethritis. A total of 210 patients (57...
  18. pmc The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-beta-D-thiogalactopyranoside exclusion and spectinomycin sensitivity
    C Bohn
    Laboratoire des Réseaux de Régulations, Institut de Genetique et Microbiologie, Universite Paris Sud, CNRS URA2225, F 91405 Orsay cedex, France
    J Bacteriol 180:6072-5. 1998
    ..The previously reported cmlA1 mutation confers a similar phenotype. cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.
  19. ncbi A steric block in translation caused by the antibiotic spectinomycin
    Maria A Borovinskaya
    Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    ACS Chem Biol 2:545-52. 2007
    The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome...
  20. pmc Ribosomal RNA and protein mutants resistant to spectinomycin
    N Bilgin
    Department of Molecular Biology, Uppsala University, Sweden
    EMBO J 9:735-9. 1990
    We have compared the influence of spectinomycin (Spc) on individual partial reactions during the elongation phase of translation in vitro by wild-type and mutant ribosomes...
  21. ncbi Analysis of a genome fragment of a deep-sea uncultivated Group II euryarchaeote containing 16S rDNA, a spectinomycin-like operon and several energy metabolism genes
    David Moreira
    UMR CNRS 8079, Ecologie, Systematique et Evolution, Universite Paris Sud, Batiment 360, 91405 Orsay Cedex, France
    Environ Microbiol 6:959-69. 2004
    ..This genome fragment exhibits a compact organization, including a few overlapping genes in the canonical spectinomycin-like (spc) operon for ribosomal proteins that is immediately upstream the 16S rDNA...
  22. ncbi The gene cluster for spectinomycin biosynthesis and the aminoglycoside-resistance function of spcM in Streptomyces spectabilis
    Kyoung Rok Kim
    Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Yongin, Gyeonggi Do, Korea
    Curr Microbiol 57:371-4. 2008
    The gene cluster for spectinomycin biosynthesis from Streptomyces spectabilis was analyzed completely and registered under the accession number EU255259 at the National Center for Biotechnology Information...
  23. ncbi Antimicrobial susceptibility among Neisseria gonorrhoeae in New Zealand in 2002
    Helen Heffernan
    LabPlus, Auckland District Health Board, New Zealand
    N Z Med J 117:U817. 2004
    ..To estimate the prevalence of antimicrobial resistance among Neisseria gonorrhoeae, and to determine whether the increase in ciprofloxacin resistance observed in Auckland in 2001 had occurred in other parts of the country...
  24. pmc In vitro effects of spectinomycin and ceftriaxone alone or in combination with other antibiotics against Chlamydia trachomatis
    Shuxian Shang
    National Center for STD and Leprosy Control, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
    Antimicrob Agents Chemother 49:1584-6. 2005
    The in vitro effects of spectinomycin and ceftriaxone, alone or in combination with erythromycin, ofloxacin, and doxycycline, against Chlamydia trachomatis were investigated by the checkerboard method and compared by Ridit (reference ..
  25. ncbi A plasmid-encoded class 1 integron carrying sat, a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan
    Ashraf M Ahmed
    Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, 1 4 4 Kagamiyama, Higashi Hiroshima, Hiroshima 739 8528, Japan
    FEMS Microbiol Lett 235:243-8. 2004
    ..and the last is an aminoglycoside adenyltransferase gene, aadA2, which confers resistance to streptomycin and spectinomycin. The putative PSP gene product was found to be 39%, 38%, 28%, and 27% identical to PSP gene products of Vibrio ..
  26. ncbi Analysis of gene cassettes of streptomycin-spectinomycin resistance of Hungarian Salmonella enterica serotype Typhimurium strains
    Noémi Nógrády
    Veterinary Medical Research Institute, Hungarian Academy of Sciences, H 1581 Budapest, P O Box 18, Hungary
    Acta Vet Hung 51:137-51. 2003
    By PCR using the ant(3")-Ia primer pair the aadA gene was detected in 34 streptomycin- and spectinomycin-resistant Salmonella enterica serotype Typhimurium strains. Out of them 12 belonged to DT104 and 22 to non-DT104 phage type...
  27. ncbi Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea
    Marina Skarjinskaia
    Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854 8020, USA
    Transgenic Res 12:115-22. 2003
    ..Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light...
  28. ncbi Antibiotic multiresistance plasmid pRSB101 isolated from a wastewater treatment plant is related to plasmids residing in phytopathogenic bacteria and carries eight different resistance determinants including a multidrug transport system
    Rafael Szczepanowski
    Fakultat fur Biologie, Lehrstuhl fur Genetik, Universitat Bielefeld, Postfach 100131, D 33501 Bielefeld, Germany
    Microbiology 150:3613-30. 2004
    ..pRSB101, mediates resistance to tetracycline, erythromycin, roxythromycin, sulfonamides, cephalosporins, spectinomycin, streptomycin, trimethoprim, nalidixic acid and low concentrations of norfloxacin...
  29. ncbi Frequency of alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in 19 clinical isolates of Neisseria gonorrhoeae in Tokyo in 2002
    Hiroyuki Abe
    Department of Urology, Nippon Medical School, Tokyo, Japan
    J Infect Chemother 9:310-3. 2003
    ..063 and 1 micro g/ml, and they increased to 4 and 8 micro g/ml, respectively, in the present study. All 19 cases of gonoccocal urethritis in the present study were cured with a single intramuscular injection of 2 g spectinomycin.
  30. ncbi Hypermutable Haemophilus influenzae with mutations in mutS are found in cystic fibrosis sputum
    Michael E Watson
    Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA 98109, USA
    Microbiology 150:2947-58. 2004
    ..isolates were screened for an increased frequency of mutation to resistance to rifampicin, nalidixic acid and spectinomycin: of the 14 hypermutable isolates identified, 12 were isolated from cystic fibrosis (CF) sputum...
  31. ncbi Spectinomycin resistance in rpsE mutants is recessive in Streptomyces roseosporus
    Xiaowei He
    Cubist Pharmaceuticals, Inc, Lexington, MA 02421, USA
    J Antibiot (Tokyo) 58:284-8. 2005
    Eight spontaneous mutants of Streptomyces roseosporus resistant to spectinomycin (SpcR) were mapped to distinct transversions or deletions in the rpsE gene. Most of the mutations were strongly recessive to the wild type SpcS allele...
  32. ncbi [Antibiotic susceptibility of Neisseria gonorrhoeae epidemic strains in Changsha]
    Ming xiang Zou
    Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China
    Hunan Yi Ke Da Xue Xue Bao 28:53-5. 2003
    ..To evaluate the antibiotic susceptibility of Neisseria gonorrhoeae epidemic strains in Changsha and to provide a reference for the prevention and treatment of gonorrhea...
  33. ncbi [Clinical study of male urethritis in Oogaki Municipal Hospital]
    Hisao Komeda
    The Department of Urology, Oogaki Municipal Hospital
    Hinyokika Kiyo 51:57-60. 2005
    ..The clinical curative rate of NGCU and NGNCU was 93% (38/41) and 80% (20/25), respectively...
  34. pmc Frequency of spontaneous mutations that confer antibiotic resistance in Chlamydia spp
    Rachel Binet
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814 4799, USA
    Antimicrob Agents Chemother 49:2865-73. 2005
    ..trachomatis L2 (two rrn operons) and Chlamydophila psittaci 6BC (one rrn operon) to the aminoglycoside spectinomycin as a model...
  35. ncbi Characterization of antimicrobial resistant Salmonella Kinshasa from dairy calves in Texas
    K M Bischoff
    US Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, TX, USA
    Lett Appl Microbiol 38:140-5. 2004
    ..To determine the prevalence of antimicrobial resistance among Salmonella isolated from a central Texas dairy calf farm that raises animals for dairy-beef production...
  36. ncbi Frequency of alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in 19 clinical isolates of Neisseria gonorrhoeae in Tokyo in 2002
    Kenichi Sato
    J Infect Chemother 9:361. 2003
  37. pmc Dual spectinomycin-streptomycin resistance marker in Brucella spp
    Jon P Woods
    Infect Immun 76:3357; author reply 3357. 2008
  38. pmc aadA confers streptomycin resistance in Borrelia burgdorferi
    Kristi L Frank
    Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA
    J Bacteriol 185:6723-7. 2003
    ..Borrelia burgdorferi, we assayed the aadA gene for the ability to confer resistance to the antibiotics spectinomycin and streptomycin...
  39. pmc Antimicrobial susceptibility of Neisseria gonorrhoeae strains isolated in Guangzhou, China, 1996-2001
    H P Zheng
    Guangzhou Center for STD Control and Prevention, Guangzhou, P R China
    Sex Transm Infect 79:399-402. 2003
    ..To investigate the in vitro antimicrobial susceptibility and resistant trends of Neisseria gonorrhoeae strains isolated in Guangzhou, from 1996 to 2001...
  40. ncbi [Resistance of Neisseria gonorrhoeae to 4 antibiotics: an analysis of the situation in Guangzhou in 2002]
    Shi Fei
    Institute of Skin Disease Prevention and Therapy of Guangzhou, Guangzhou 510095, China
    Di Yi Jun Yi Da Xue Xue Bao 23:1099-100. 2003
    ..To survey the antibiotic resistance and the prevalence of penicillinase-producing and highly tetracycline-resistant Neisseria gonorrhoeae (PPNG and TRNG) in Guangzhou in 2002...
  41. ncbi Non-penicillinase producing Neisseria gonnorhoeae: are they still in existence in Ibadan, Nigeria?
    R A Bakare
    Special Treatment Clinic, University College Hospital, Ibadan, Nigeria
    Afr J Med Med Sci 30:281-3. 2001
    ..The present study revealed that non-PPNG strains have reduced considerably to 5.4% from 100% in 1977. This has posed a great threat to the usefulness of penicillin and ampicillin as the drugs of choice in gonococcal therapy in Nigeria...
  42. pmc Persistence of unselected transgenic DNA during a plastid transformation and segregation approach to herbicide resistance
    Guang Ning Ye
    Monsanto Company, St Louis, Missouri 63198, USA
    Plant Physiol 133:402-10. 2003
    The use of a nonlethal selection scheme, most often using the aadA gene that confers resistance to spectinomycin and streptomycin, has been considered critical for recovery of plastid transformation events...
  43. ncbi A pilot study on antimicrobial susceptibility of Neisseria gonorrhoeae isolates from Nepal
    Chintamani Chaudhary
    AMDA, Kathmandu, Nepal
    Sex Transm Dis 32:641-3. 2005
  44. ncbi Isolation of spectinomycin resistance mutations in the 16S rRNA of Salmonella enterica serovar Typhimurium and expression in Escherichia coli and Salmonella
    MICHAEL O'CONNOR
    J W Wilson Laboratory, Department of Molecular and Cellular Biology and Biochemistry, Brown University, 69 Brown Street, Providence RI 02912, USA
    Curr Microbiol 45:429-33. 2002
    Two single-base mutations in 16S rRNA conferring high-level resistance to spectinomycin were isolated on a plasmid-borne copy of the rrnD operon from Salmonella enterica serovar Typhimurium...
  45. pmc Novel streptomycin and spectinomycin resistance gene as a gene cassette within a class 1 integron isolated from Escherichia coli
    D Sandvang
    Danish Veterinary Laboratory, DK 1790 Copenhagen V, and Department of Microbiology, The Royal Veterinary and Agricultural University, DK 1870 Frederiksberg C, Denmark
    Antimicrob Agents Chemother 43:3036-8. 1999
    The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species...
  46. ncbi Contribution of the Rv2333c efflux pump (the Stp protein) from Mycobacterium tuberculosis to intrinsic antibiotic resistance in Mycobacterium bovis BCG
    Santiago Ramón-García
    Departamento de Microbiologia, Medicina Preventiva y Salud Pública, Universidad de Zaragoza, 50009 Zaragoza, Spain
    J Antimicrob Chemother 59:544-7. 2007
    ..To characterize the efflux pump encoded by the gene Rv2333c from Mycobacterium tuberculosis, and assess its contribution to intrinsic antibiotic resistance using Mycobacterium bovis BCG as a model organism...
  47. pmc Mutations in 16S rRNA and ribosomal protein S5 associated with high-level spectinomycin resistance in Pasteurella multocida
    Corinna Kehrenberg
    Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft FAL, Höltystr 10, 31535 Neustadt Mariensee, Germany
    Antimicrob Agents Chemother 51:2244-6. 2007
    Pasteurella multocida isolates with high-level spectinomycin resistance in which no adenyltransferase genes could be demonstrated exhibited a C1192G transversion in the 16S rRNA of all six or five of the six rRNA operons and/or two ..
  48. ncbi Activity of translation system and abundance of tmRNA during development of Streptomyces aureofaciens producing tetracycline
    P Palecková
    Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia
    Folia Microbiol (Praha) 51:517-24. 2006
    ..These experiments suggest that the main role of the rising tmRNA level at the beginning of the Ttc production is connected with ribosome rescue...
  49. ncbi Antimicrobial resistance for Neisseria gonorrhoeae in the United States, 1988 to 2003: the spread of fluoroquinolone resistance
    Susan A Wang
    National Center for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Emory University School of Medicine, and Emory Center for AIDS Research, Atlanta, Georgia 30333, USA
    Ann Intern Med 147:81-8. 2007
    ..Over the past 60 years, Neisseria gonorrhoeae has acquired clinically significant resistance to sulfonamides, tetracyclines, penicillins, and ciprofloxacin...
  50. ncbi Characterization of antimicrobial susceptibility and virulence genes of Salmonella serovars collected at a commercial turkey processing plant
    C W Nde
    Department of Veterinary and Microbiological Sciences, The Great Plains Institute of Food Safety, North Dakota State University, Fargo, ND 58105, USA
    J Appl Microbiol 104:215-23. 2008
    ....
  51. ncbi Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen
    S Akhter
    Department of Zoology, University of Arid Agriculture, Rawalpindi, Pakistan
    Reprod Domest Anim 43:272-8. 2008
    ..and penicillin; SP) and relatively modern combination of antibiotics (gentamycin, tylosin, lincomycin and spectinomycin; GTLS) in extender on bacterial control and spermatozoal quality of liquid buffalo bull semen stored at 5 ..
  52. pmc Revised analysis of aadA2 gene of plasmid pSa
    A Bito
    Institut für Genetik und Allgemeine Biologie, Universitat Salzburg, Austria
    Antimicrob Agents Chemother 38:1172-5. 1994
    ..These deviations are located in the coding region and in the 3' noncoding region. By making deletions in the region for initiation of protein synthesis, we identified a GTG triplet as the most probable start codon for translation...
  53. ncbi An efficient approach for cloning the dNDP-glucose synthase gene from actinomycetes and its application in Streptomyces spectabilis, a spectinomycin producer
    C Hyun
    Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University, Yongin, South Korea
    FEMS Microbiol Lett 183:183-9. 2000
    ..In addition, we have cloned a 45-kb region of DNA from Streptomyces spectabilis ATCC27741, a spectinomycin producer which contained the dTDP-glucose synthase and dTDP-glucose 4,6-dehydratase genes named spcD and spcE, ..
  54. ncbi Susceptibility of Ureaplasma urealyticum to ten chemotherapeutic agents
    R Sobetzko
    , ,
    Zentralbl Bakteriol Mikrobiol Hyg A 269:245-50. 1988
    ..0, erythromycin and streptomycin-4.0, chloramphenicol, gentamicin, rosoxacin-8.0, spectinomycin-32.0...
  55. pmc The region of the IncN plasmid R46 coding for resistance to beta-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found in IncW plasmids and in Tn21-like transposons
    R M Hall
    CSIRO Division of Molecular Biology, North Ryde, NSW, Australia
    Nucleic Acids Res 15:7491-501. 1987
    ..The possibility that this antibiotic resistance segment is a discrete mobile DNA element is discussed...
  56. ncbi Susceptibility of bacterial pathogens against lincomycin/spectinomycin (1/2), penicillin G/neomycin (1/1), and penicillin G/dihydrostreptomycin (1/1) as determined in the BfT-GermVet monitoring program 2004-2006
    Stefan Schwarz
    Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft, Neustadt Mariensee
    Berl Munch Tierarztl Wochenschr 120:363-71. 2007
    ..were investigated for their susceptibility to the three combinations of antimicrobial agents lincomycin/spectinomycin (1/2), penicillin G/neomycin (1/1), and penicillin G/dihydrostreptomycin (1/1) in comparison to their ..
  57. ncbi Gonococcal resistance: evolving from penicillin, tetracycline to the quinolones in South Africa -- implications for treatment guidelines
    Mari De Jongh
    Department of Microbiological Pathology, University of Limpopo Medunsa Campus, Ga Rankuwa, Medunsa, South Africa
    Int J STD AIDS 18:697-9. 2007
    ..Overall, the gonococcal isolates in the Pretoria region remain susceptible to ceftriaxone, cefoxitin, cefpodoxime and spectinomycin.
  58. doi National surveillance of antimicrobial susceptibility in Neisseria gonorrhoeae in 2005-2006 and recommendations of first-line antimicrobial drugs for gonorrhoea treatment in Russia
    A Kubanova
    Central Research Institute of Dermato Venereology, Ministry of Health, Moscow, Russia
    Sex Transm Infect 84:285-9. 2008
    ..To investigate comprehensively the antimicrobial susceptibility and resistance of Neisseria gonorrhoeae during 2005-2006 in a national survey and to recommend effective antimicrobial drugs for the treatment of gonorrhoea in Russia...
  59. pmc Variant Salmonella genomic island 1-L antibiotic resistance gene cluster in Salmonella enterica serovar Newport
    Axel Cloeckaert
    Unité Infectiologie Animale et Santé Publique site 213, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
    Antimicrob Agents Chemother 50:3944-6. 2006
    ..The isolate carried another class 1 integron containing the aacC5 and aadA7 gene cassettes conferring resistance to gentamicin and streptomycin/spectinomycin, respectively.
  60. ncbi Development of a method for heterologous gene expression in Enterobacter amnigenus, a potential host for the biological control of mosquito larvi
    Nawarat Nantapong
    Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakornpathom, 73170 Thailand
    J Microbiol Methods 49:329-34. 2002
    ..3 kb fragment of chromosomal DNA from Enterobacter amnigenus was constructed. The Omega fragment encoding spectinomycin/streptomycin resistance was cloned into the unique BglII site of the resulting plasmid, and the interrupted ..
  61. ncbi Comparison of Neisseria gonorrhoeae isolates from the genital tract and pharynx of two gonorrhea patients
    T Saika
    Chemotherapy Division, Mitsubishi Kagaku Bio-Clinical Laboratories, 3-30-1 Shimura, Itabashi-ku, Tokyo 174-8555, Japan
    J Infect Chemother 7:175-9. 2001
    ..This method may contribute to the successful treatment and epidemiological investigation of gonococcal infections...
  62. ncbi Antibiotic resistant N. gonorrhoeae in Trinidad and Tobago
    D Castor
    Department of Epidemiology and International Health, School of Public Health and Medical Statistics Section, University of Alabama at Birmingham, 35294-0022, USA
    Cell Mol Biol (Noisy-le-grand) 47:987-95. 2001
    ..gonorrhoeae isolates to six antimicrobials; penicillin, tetracycline, spectinomycin, ceftriaxone, ciprofloxacin and azithromycin, and examined whether certain demographic or behavioral factors ..
  63. ncbi Antibiotic susceptibility patterns of Neisseria gonorrhoeae isolates in Port Elizabeth
    S Govender
    Department of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, Port Elizabeth, Eastern Cape, South Africa
    S Afr Med J 96:225-6. 2006
    ..To survey the antibiotic susceptibility of Neisseria gonorrhoeae isolates...
  64. pmc In vitro mutagenesis of Bacillus subtilis by using a modified Tn7 transposon with an outward-facing inducible promoter
    Christophe Bordi
    Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853 8101, USA
    Appl Environ Microbiol 74:3419-25. 2008
    ..This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated ..
  65. ncbi The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100
    Andreas Tauch
    Degussa AG, Kantstrasse 2, D 33790 Halle Künsebeck, Germany
    Plasmid 48:117-29. 2002
    ..8-kb R-plasmid pTET3 from Corynebacterium glutamicum LP-6 which encodes streptomycin, spectinomycin, and tetracycline resistance...
  66. ncbi Emergence of cephem- and aztreonam-high-resistant Neisseria gonorrhoeae that does not produce beta-lactamase
    S Akasaka
    Department of Urology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan
    J Infect Chemother 7:49-50. 2001
    ..These isolates were susceptible to spectinomycin. In N. gonorrhoeae, the emergence of these beta-lactam-resistant isolates is of serious concern...
  67. pmc Influences of biofilm structure and antibiotic resistance mechanisms on indirect pathogenicity in a model polymicrobial biofilm
    Heather A O'Connell
    Department of Biology, Georgia State University, Atlanta, GA 30303, USA
    Appl Environ Microbiol 72:5013-9. 2006
    ..Escherichia coli ATCC 33456 was transformed with vectors conferring either ampicillin or spectinomycin resistance, creating two distinct populations with different resistance mechanisms...
  68. ncbi PsbI affects the stability, function, and phosphorylation patterns of photosystem II assemblies in tobacco
    Serena Schwenkert
    Department Biology I, Botany, Ludwig Maximilians University Munich, Menzingerstrasse 67, 80638 Munich, Germany
    J Biol Chem 281:34227-38. 2006
    ..higher plants, we generated homoplastomic knock-out plants by replacing most of the tobacco psbI gene with a spectinomycin resistance cartridge...
  69. ncbi Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics
    A P Carter
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 407:340-8. 2000
    ..describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation...
  70. ncbi [Antimicrobial resistance in Neisseria gonorrheae in North-East Romania]
    Aida Corina Bădescu
    Facultatea de Medicina, Disciplina de Microbiologie, Universitatea de Medicină si Farmacie Gr T Popa Iaşi
    Rev Med Chir Soc Med Nat Iasi 111:512-5. 2007
    ..This study is performed to evaluate evolution of antimicrobial resistance patterns of N. gonorrhoeae isolates from north-east Romania...
  71. pmc Cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from Bordetella avium and expression of the gene in Salmonella typhimurium
    C R Gentry-Weeks
    Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland 20892
    J Bacteriol 174:7729-42. 1992
    ..in Escherichia coli LE392 by using the cosmid vectors pCP13 and pYA2329, a derivative of pCP13 specifying spectinomycin resistance. The cosmid libraries were screened with convalescent-phase anti-B...
  72. pmc Characterization of Salmonella enterica serovar typhimurium DT104 isolated from Denmark and comparison with isolates from Europe and the United States
    D L Baggesen
    Danish Veterinary Laboratory, DK 1790 Copenhagen V, Denmark
    J Clin Microbiol 38:1581-6. 2000
    ..All but 10 isolates were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfonamides, and tetracycline...
  73. ncbi Fluoroquinolone-resistant Neisseria gonorrhoeae in Bali, Indonesia: 2004
    Elizabeth A Donegan
    University of California, San Francisco, 94143, USA
    Sex Transm Dis 33:625-9. 2006
    ..To assess the importance of this observation, we determined antimicrobial susceptibilities and strain types of N. gonorrhoeae isolates from FSWs seen in a sexually transmitted infection (STI) clinic in Denpasar, Bali, Indonesia...
  74. ncbi Identification of plasmid-mediated extended-spectrum and AmpC beta-lactamases in Enterobacter spp. isolated from dogs
    Hanna E Sidjabat
    School of Veterinary Science, The University of Queensland, Brisbane, QLD 4072, and Microbiology and Infectious Diseases, Women s and Children s Hospital, North Adelaide, SA, Australia
    J Med Microbiol 56:426-34. 2007
    ..plasmid (approximately 140 kb) that also conferred resistance to chloramphenicol, gentamicin, spectinomycin, tetracycline, trimethoprim and sulfonamides...
  75. ncbi Reduced susceptibility to azithromycin and high percentages of penicillin and tetracycline resistance in Neisseria gonorrhoeae isolates from Manaus, Brazil, 1998
    J A Dillon
    Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Canada
    Sex Transm Dis 28:521-6. 2001
    ..several reports document resistance to third-generation cephalosporins, and the sporadic isolation of spectinomycin-resistant isolates continues unabated...
  76. ncbi Antimicrobial susceptibility of Neisseria gonorrhoeae in Cuba (1995-1999): implications for treatment of gonorrhea
    Rafael Llanes
    Microbiology Branch, Tropical Medicine Institute Pedro Kouri, Havana, Cuba
    Sex Transm Dis 30:10-4. 2003
    ..Antibiotic-resistant strains of Neisseria gonorrhoeae, especially those resistant to penicillin and tetracycline, have spread with remarkable rapidity in many Caribbean countries...
  77. ncbi tet(L)-mediated tetracycline resistance in bovine Mannheimia and Pasteurella isolates
    Corinna Kehrenberg
    Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft FAL, Höltystr 10, 31535 Neustadt Mariensee, Germany
    J Antimicrob Chemother 56:403-6. 2005
    ..Tetracycline-resistant Mannheimia and Pasteurella isolates, which were negative for the tetracycline resistance genes (tet) commonly detected among these bacteria, were investigated for other tet genes present and their location...
  78. ncbi Antimicrobial susceptibilities of Neisseria gonorrhoeae strains isolated in Java, Indonesia
    Margareta Ieven
    Department of Medical Microbiology, University Hospital Antwerp, UIA, Antwerp, Belgium
    Sex Transm Dis 30:25-9. 2003
    ..Despite the rapid spread of antibiotic resistance in gonococci all over Southeast Asia, there is only limited surveillance for antibiotic susceptibility in Indonesia...
  79. pmc The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen
    Michael E Reith
    NRC Institute for Marine Biosciences, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada
    BMC Genomics 9:427. 2008
    ..salmonicida subsp. salmonicida causes disease in healthy fish. The genome sequence of A. salmonicida was determined to provide a better understanding of the virulence factors used by this pathogen to infect fish...
  80. pmc Effects of flavophospholipol on resistance in fecal Escherichia coli and enterococci of fattening pigs
    A E van den Bogaard
    Department of Medical Microbiology, University Maastricht, NL 6200 MD Maastricht, The Netherlands
    Antimicrob Agents Chemother 46:110-8. 2002
    ..after disturbance of the colonization resistance of the intestinal flora by a 3-day course of lincomycin and spectinomycin. At the end of the study, the overall prevalence and degree of resistance of E...
  81. pmc Molecular epidemiology of sequential outbreaks of Acinetobacter baumannii in an intensive care unit shows the emergence of carbapenem resistance
    Raffaele Zarrilli
    Dipartimento di Scienze Mediche Preventive, Universita di Napoli Federico II, Naples, Italy
    J Clin Microbiol 42:946-53. 2004
    ..two open reading frames (ORFs) coding for unknown products; and aadA1a, which confers resistance to spectinomycin and streptomycin...
  82. ncbi Class 1 integrons and resistance gene cassettes among multidrug resistant Salmonella serovars isolated from slaughter animals and foods of animal origin in Ethiopia
    Bayleyegn Molla
    Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Addis Ababa University, P O Box 34, Debre Zeit, Ethiopia
    Acta Trop 103:142-9. 2007
    ..6%), tetracycline (75.5%), sulfamethoxazole (60.2%), spectinomycin (53.1%), ampicillin (42.8%), nalidixic acid (34.7%), nitrofurantoin (30.6%), trimethoprim (27...
  83. ncbi Application of flow cytometry for the determination of minimal inhibitory concentration of several antibacterial agents on Mycoplasma hyopneumoniae
    P Assunção
    Unidad de Epidemiologia y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Arucas, Spain
    J Appl Microbiol 102:1132-7. 2007
    ..of the minimal inhibitory concentrations (MICs) of nine antibacterial agents (enrofloxacin, ciprofloxacin, oxytetracycline, chloramphenicol, tylosin, lincomycin, gentamycin, spectinomycin and streptomycin) against M. hyopneumoniae.
  84. pmc Antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, Russia
    Veronica Vorobieva
    Department of Microbiology and Virology, University of Tromsø, Tromsø, Norway
    Sex Transm Infect 83:133-5. 2007
    ..To characterise comprehensively the antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, Russia, and to investigate whether the recommended treatment guidelines are updated and effective...
  85. pmc Identification and characterization of integron-mediated antibiotic resistance among Shiga toxin-producing Escherichia coli isolates
    S Zhao
    Division of Animal and Food Microbiology, Office of Research, U S Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, MD 20708, USA
    Appl Environ Microbiol 67:1558-64. 2001
    ..O111:NM, O45:H2, and O26:H11 isolates contained the aadA gene encoding resistance to streptomycin and spectinomycin. Integrons identified among the O157:H7 and O103:H2 isolates also possessed a similar aadA gene...
  86. ncbi Integrons and gene cassettes in clinical isolates of co-trimoxazole-resistant Gram-negative bacteria
    M Grape
    Microbiology and Tumor Biology Center MTC, Clinical Microbiology, Karolinska Institute, Karolinska Hospital L2 02, Stockholm SE 171 76, Sweden
    Clin Microbiol Infect 11:185-92. 2005
    ..Thus, only resistance to trimethoprim, streptomycin, spectinomycin and sulphonamides could be explained by the presence of integrons in these isolates...
  87. pmc Molecular characterization of plasmid pBM300 from Bacillus megaterium QM B1551
    Muthusamy Kunnimalaiyaan
    Department of Biological Sciences, Northern Illinois University, De Kalb 60115, USA
    Appl Environ Microbiol 71:3068-76. 2005
    ..The entire plasmid was isolated from the plasmid array by integrating a spectinomycin resistance gene and transforming a plasmidless strain, PV361...
  88. pmc Molecular epidemiology and antimicrobial resistance of Salmonella typhimurium DTI04 on Ontario swine farms
    Abdolvahab Farzan
    Department of Population Medicine, University of Guelph, Guelph, Ontario N1G 2W1
    Can J Vet Res 72:188-94. 2008
    ..antimicrobials with the most frequent resistance being to sulfonamides (Su), ampicillin (A), streptomycin (S), spectinomycin (Sp), chloramphenicol (C), tetracycline (T), and florfenicol (F)...
  89. doi Prediction of antibiotic resistance using Neisseria gonorrhoeae multi-antigen sequence typing
    H M Palmer
    Scottish Bacterial Sexually Transmitted Infections Reference Laboratory SBSTIRL, Department of Medical Microbiology, Royal Infirmary of Edinburgh, 51 Little France Crescent, Edinburgh EH16 5SA, UK
    Sex Transm Infect 84:280-4. 2008
    ..To establish whether antibiotic resistance in Neisseria gonorrhoeae is uniform within a given sequence type as determined by N gonorrhoeae multi-antigen sequence typing (NG-MAST)...
  90. ncbi Tendency toward increase in the frequency of isolation of beta-lactamase-nonproducing Neisseria gonorrhoeae exhibiting penicillin resistance, and recent emergence of multidrug-resistant isolates in Japan
    Intetsu Kobayashi
    Chemotherapy Division, Mitsubishi Kagaku Bio Clinical Laboratories, Tokyo, Japan
    J Infect Chemother 9:126-30. 2003
    ..to their beta-lactamase production and susceptibility to penicillin G, cefixime, ceftriaxone, tetracycline, spectinomycin, ciprofloxacin, and azithromycin. The frequency of isolation of penicillinase-producing N...
  91. ncbi Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study
    Kristina Kadlec
    Institute of Farm Animal Genetics, Friedrich Loeffler Institute, Höltystr 10, 31535 Neustadt Mariensee, Germany
    J Antimicrob Chemother 62:469-73. 2008
    ..This study dealt with the identification of integron-associated resistance genes among these isolates...
  92. ncbi Development of a toxicogenomics in vitro assay for the efficient characterization of compounds
    Yi Yang
    Abbott Laboratories, Department of Cellular and Molecular Toxicology, 100 Abbott Park Rd, Abbott Park, IL 60064 6123, USA
    Pharmacogenomics 7:177-86. 2006
    ..bezafibrate, clofibrate, and Wy-14643 as peroxisome proliferators, and chlorpheniramine, penicillin and spectinomycin as negative controls...
  93. pmc Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis
    N C Clark
    Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Antimicrob Agents Chemother 43:157-60. 1999
    Genes encoding streptomycin/spectinomycin adenylyltransferases [ANT(3")(9)] have been reported to exist in gram-negative organisms and Staphylococcus aureus...
  94. pmc Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei
    R A Moore
    Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada
    Antimicrob Agents Chemother 43:465-70. 1999
    ..their susceptibilities to the aminoglycosides streptomycin, gentamicin, neomycin, tobramycin, kanamycin, and spectinomycin. In addition, both mutants, in contrast to the parent, were susceptible to the macrolides erythromycin and ..
  95. ncbi Antibiotic susceptibility of Riemerella anatipestifer field isolates
    Chong Yue Zhong
    Avian Disease Research Centre, College of Veterinary Medicine, Sichuan Agriculture University, Ya an, Sichuan, 625014, China
    Avian Dis 53:601-7. 2009
    ..anatipestifer to cefoperazone, piperacillin, spectinomycin, and aztreonam. In addition to determining the antibiotic-resistance profiles of R...
  96. ncbi Salmonella enterica serotype Typhimurium carrying hybrid virulence-resistance plasmids (pUO-StVR): a new multidrug-resistant group endemic in Spain
    Ana Herrero
    Departamento de Biologia Funcional, Area de Microbiologia, Facultad de Medicina, University of Oviedo, C Julián Clavería No 6, E 33006 Oviedo, Spain
    Int J Med Microbiol 298:253-61. 2008
    ..isolates (>89%) shared the following features: (i) resistance to ampicillin, chloramphenicol, streptomycin/spectinomycin, sulfonamides, and tetracycline, encoded by bla(OXA-1)-catA1-aadA1-sul1-tet(B); (ii) a class 1 integron (InH) ..
  97. ncbi Correlation between In vitro susceptibility and treatment outcome with azithromycin in gonorrhoea: a prospective study
    P Khaki
    Department of Microbiology, Maulana Azad Medical College, New Delhi 110 002, India
    Indian J Med Microbiol 25:354-7. 2007
    ..Also, the correlation between in vitro susceptibility and treatment outcome with single 2 g oral dose azithromycin was assessed...
  98. ncbi Comparative analysis of antibiotic resistance characteristics of Gram-negative bacteria isolated from laying hens and eggs in conventional and organic keeping systems in Bavaria, Germany
    K Schwaiger
    Technische Universitat Munchen, Munich, Germany
    Zoonoses Public Health 55:331-41. 2008
    ..of the serogroup B (n=4) up to six antibiotics. All tested Salmonella (n=23) proved to be resistant to spectinomycin. Escherichia coli (n=257(org) and 276(con)) from organic layers showed significant lower resistance rates and ..
  99. pmc Impact of feed supplementation with antimicrobial agents on growth performance of broiler chickens, Clostridium perfringens and enterococcus counts, and antibiotic resistance phenotypes and distribution of antimicrobial resistance determinants in Escheric
    Moussa S Diarra
    Pacific Agri Food Research Centre, Agriculture and Agri Food Canada, Agassiz, British Columbia, Canada
    Appl Environ Microbiol 73:6566-76. 2007
    ..Resistance to tetracycline (68.5%), amoxicillin (61.4%), ceftiofur (51.3%), spectinomycin (47.2%), and sulfonamides (42%) was most frequent...
  100. pmc Chloroplast-encoded chlB is required for light-independent protochlorophyllide reductase activity in Chlamydomonas reinhardtii
    J Li
    Department of Biology, University of Virginia, Charlottesville 22903
    Plant Cell 5:1817-29. 1993
    ..reduction in this alga, a series of plasmids were constructed in which the aadA gene conferring spectinomycin resistance was inserted at three different sites within the chlB gene...
  101. pmc Antibiotic resistance in food-borne bacterial contaminants in Vietnam
    Thi Thu Hao Van
    School of Applied Sciences, RMIT University, Building 3, Level 1, Room 2, City Campus, Melbourne, Victoria 3001, Australia
    Appl Environ Microbiol 73:7906-11. 2007
    ..integrons harbored various gene cassettes, including aadA1, aadA2, and aadA5 (resistance to streptomycin and spectinomycin), aacA4 (resistance to aminoglycosides), the dihydrofolate reductase gene cassettes dhfrXII, dfrA1, and ..

Research Grants55

  1. SITE SPECIFIC RECOMBINATION IN STAPHYLOCOCCUS AUREUS
    Ellen Murphy; Fiscal Year: 1990
    Tn554 is a transposable element in Staphylococcus aureus that encodes resistances to spectinomycin and to the macrolide-lincosamide-streptogramin B antibiotics. Tn554 inserts efficiently into a unique site in the S. aureus chromosome...
  2. Development of novel proteins synthesis inhibitors for MDR tuberculosis
    RICHARD EDWARD LEE; Fiscal Year: 2010
    ..We focused on the low molecular weight aminocyclitol spectinomycin as it: has been under studied;has a safe pharmacological profile;targets a ribosomal binding site highly ..
  3. SELECTIVE ACTIONS OF ANTIBIOTICS ON RIBOSOMES
    PHANG TAI; Fiscal Year: 1980
    ..We have found that streptomycin, spectinomycin, and erythromycin block initiating ribosomes at some step, but at different stages, shortly after formation of ..
  4. POLYSOMES & SUBUNITS: STRUCTURE-FUNCTION RELATIONSHIPS
    ALBERT DAHLBERG; Fiscal Year: 1990
    ..sites for missense suppression, regions in 16S and 23S rRNA which confer resistance to antibiotics such as spectinomycin, streptomycin, the amino-glycosides, and thiostrepton, and finally regions in 16S rRNA which are involved in ..
  5. POLYSOMES & SUBUNITS: STRUCTURE-FUNCTION RELATIONSHIPS
    ALBERT DAHLBERG; Fiscal Year: 1992
    ..sites for missense suppression, regions in 16S and 23S rRNA which confer resistance to antibiotics such as spectinomycin, streptomycin, the amino-glycosides, and thiostrepton, and finally regions in 16S rRNA which are involved in ..
  6. POLYSOMES & SUBUNITS: STRUCTURE-FUNCTION RELATIONSHIPS
    ALBERT DAHLBERG; Fiscal Year: 1991
    ..sites for missense suppression, regions in 16S and 23S rRNA which confer resistance to antibiotics such as spectinomycin, streptomycin, the amino-glycosides, and thiostrepton, and finally regions in 16S rRNA which are involved in ..
  7. MOLECULAR AND FUNCTIONAL STUDIES OF H INFLUENZAE B PILI
    Janet Gilsdorf; Fiscal Year: 1992
    ..Pili deficient (p-) mutants are being constructed by insertion of the 2 kb omega fragment encoding spectinomycin and streptomycin resistance...
  8. MOLECULAR AND FUNCTIONAL STUDIES OF H INFLUENZAE B PILI
    Janet Gilsdorf; Fiscal Year: 1993
    ..Pili deficient (p-) mutants are being constructed by insertion of the 2 kb omega fragment encoding spectinomycin and streptomycin resistance...
  9. MOLECULAR AND FUNCTIONAL STUDIES OF H INFLUENZAE B PILI
    Janet Gilsdorf; Fiscal Year: 1991
    ..Pili deficient (p-) mutants are being constructed by insertion of the 2 kb omega fragment encoding spectinomycin and streptomycin resistance...
  10. TRANSPOSITION OF TN7
    Nancy Craig; Fiscal Year: 1990
    ..of the bacterial transposition Tn7 which encodes resistances to trimethoprim and to streptomycin and spectinomycin. In contrast to most other transposable elements which move at low frequency and show little target site ..
  11. TRANSPOSITION OF TN7
    Nancy Craig; Fiscal Year: 1999
    ..of the bacterial transposition Tn7 which encodes resistances to trimethoprim and to streptomycin and spectinomycin. In contrast to most other transposable elements which move at low frequency and show little target site ..
  12. NUCLEIC ACID STRUCTURE/FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 1999
    ..The approach is to use a 16S rRNA mutant with an altered anti-Shine-Dalgarno sequence to direct entry of a spectinomycin-resistant ribosome selectively to the upstream gene, which has been provided a Shine-Dalgarno sequence ..
  13. NUCLEIC ACID STRUCTURE/FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 2000
    ..The approach is to use a 16S rRNA mutant with an altered anti-Shine-Dalgarno sequence to direct entry of a spectinomycin-resistant ribosome selectively to the upstream gene, which has been provided a Shine-Dalgarno sequence ..
  14. STRUCTURE AND MECHANISM OF RIBOSOMAL RNA IN THE RIBSOME
    Paul Wollenzien; Fiscal Year: 1999
    ..and 70S ribosome; (2) Determine the details of the structural changes that occur during tetracycline and spectinomycin binding and determine how these changes interfere with tRNA binding and the translocation process; (3) ..
  15. STRUCTURE AND MECHANISM OF RIBOSOMAL RNA IN THE RIBSOME
    Paul Wollenzien; Fiscal Year: 2002
    ..and 70S ribosome; (2) Determine the details of the structural changes that occur during tetracycline and spectinomycin binding and determine how these changes interfere with tRNA binding and the translocation process; (3) ..
  16. STRUCTURE AND MECHANISM OF RIBOSOMAL RNA IN THE RIBSOME
    Paul Wollenzien; Fiscal Year: 2000
    ..and 70S ribosome; (2) Determine the details of the structural changes that occur during tetracycline and spectinomycin binding and determine how these changes interfere with tRNA binding and the translocation process; (3) ..
  17. NUCLEIC ACID STRUCTURE-FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 1993
    ..in vitro, using a 16S rRNA mutant with an altered anti-Shine- Dalgarno sequence to direct entry of a spectinomycin-resistant ribosome to an upstream cistron bearing the complimentary Shine-Dalgarno sequence...
  18. NUCLEIC ACID STRUCTURE-FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 1992
    ..in vitro, using a 16S rRNA mutant with an altered anti-Shine- Dalgarno sequence to direct entry of a spectinomycin-resistant ribosome to an upstream cistron bearing the complimentary Shine-Dalgarno sequence...
  19. STRUCTURE AND MECHANISM OF RIBOSOMAL RNA IN THE RIBSOME
    Paul Wollenzien; Fiscal Year: 2001
    ..and 70S ribosome; (2) Determine the details of the structural changes that occur during tetracycline and spectinomycin binding and determine how these changes interfere with tRNA binding and the translocation process; (3) ..
  20. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2004
    ..Large scale and low cost production via transgenic chloroplasts should provide treatment to patients at an affordable cost and tobacco farmers alternate uses for this hazardous crop. ..
  21. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2003
    ..Large scale and low cost production via transgenic chloroplasts should provide treatment to patients at an affordable cost and tobacco farmers alternate uses for this hazardous crop. ..
  22. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2006
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  23. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2002
    ..Large scale and low cost production via transgenic chloroplasts should provide treatment to patients at an affordable cost and tobacco farmers alternate uses for this hazardous crop. ..
  24. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2005
    ..Large scale and low cost production via transgenic chloroplasts should provide treatment to patients at an affordable cost and tobacco farmers alternate uses for this hazardous crop. ..
  25. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2008
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  26. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  27. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2009
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  28. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2007
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..
  29. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2005
    ..This work will help establish how Gc has evolved to subvert the innate immune response and allow continued transmission within infected populations. ..
  30. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2006
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..
  31. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2009
    ..This work will help establish how Gc has evolved to subvert the innate immune response and allow continued transmission within infected populations. ..
  32. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2006
    ..This work will help establish how Gc has evolved to subvert the innate immune response and allow continued transmission within infected populations. ..
  33. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2008
    ..This work will help establish how Gc has evolved to subvert the innate immune response and allow continued transmission within infected populations. ..
  34. Molecular Genetics of the gonococcus
    H Seifert; Fiscal Year: 2007
    ..This work will help establish how Gc has evolved to subvert the innate immune response and allow continued transmission within infected populations. ..
  35. Mechanisms of Gonococcal Pilin Antigenic & Phase Variation
    H Seifert; Fiscal Year: 2008
    ..Understanding the mechanisms used to direct and control pilin Av is essential to understanding this aspect of the pathogenesis of gonococcal disease. ..
  36. MECHANISMS OF GONOCOCCAL PILIN ANTIGENIC/PHASE VARIATION
    H Seifert; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  37. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2005
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..
  38. MOLECULAR ANALYSIS OF GONOCOCCAL RECA FUNCTIONS
    H Seifert; Fiscal Year: 2000
    ..These studies will aid in the understanding of gonococcal pathogenesis, and help define how the Gc and Ec RecA proteins mediate different cellular processes. ..
  39. MECHANISMS OF GONOCOCCAL PILIN ANTIGENIC/PHASE VARIATION
    H Seifert; Fiscal Year: 2000
    ..Through these investigations, this project will continue to define the molecular mechanism used to allow these high frequency recombination reactions that are of critical importance to the pathogenesis of gonorrhea. ..
  40. MECHANISMS OF GONOCOCCAL PILIN ANTIGENIC/PHASE VARIATION
    H Seifert; Fiscal Year: 1999
    ..Through these investigations, this project will continue to define the molecular mechanism used to allow these high frequency recombination reactions that are of critical importance to the pathogenesis of gonorrhea. ..
  41. MECHANISMS OF GONOCOCCAL PILIN ANTIGENIC/PHASE VARIATION
    H Seifert; Fiscal Year: 2001
    ..Through these investigations, this project will continue to define the molecular mechanism used to allow these high frequency recombination reactions that are of critical importance to the pathogenesis of gonorrhea. ..
  42. MOLECULAR ANALYSIS OF GONOCOCCAL RECA FUNCTIONS
    H Seifert; Fiscal Year: 2002
    ..These studies will aid in the understanding of gonococcal pathogenesis, and help define how the Gc and Ec RecA proteins mediate different cellular processes. ..
  43. MECHANISMS OF GONOCOCCAL PILIN ANTIGENIC/PHASE VARIATION
    H Seifert; Fiscal Year: 2002
    ..Through these investigations, this project will continue to define the molecular mechanism used to allow these high frequency recombination reactions that are of critical importance to the pathogenesis of gonorrhea. ..
  44. MOLECULAR ANALYSIS OF GONOCOCCAL RECA FUNCTIONS
    H Seifert; Fiscal Year: 2001
    ..These studies will aid in the understanding of gonococcal pathogenesis, and help define how the Gc and Ec RecA proteins mediate different cellular processes. ..
  45. MOLECULAR ANALYSIS OF GONOCOCCAL RECA FUNCTIONS
    H Seifert; Fiscal Year: 2003
    ..These studies will aid in the understanding of gonococcal pathogenesis, and help define how the Gc and Ec RecA proteins mediate different cellular processes. ..
  46. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2004
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..