nocodazole

Summary

Summary: Nocodazole is an antineoplastic agent which exerts its effect by depolymerizing microtubules.

Top Publications

  1. ncbi Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis
    M A Jordan
    Department of Biological Sciences, University of California, Santa Barbara 93106
    J Cell Sci 102:401-16. 1992
  2. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
  3. ncbi Disorganized microtubules underlie the formation of retraction bulbs and the failure of axonal regeneration
    Ali Erturk
    Max Planck Institute of Neurobiology, Axonal Growth and Regeneration, 82152 Martinsried, Germany
    J Neurosci 27:9169-80. 2007
  4. pmc Mitotic checkpoint slippage in humans occurs via cyclin B destruction in the presence of an active checkpoint
    Daniela A Brito
    Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, New York 12222, USA
    Curr Biol 16:1194-200. 2006
  5. ncbi Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
  6. pmc Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells
    Mirita Franz-Wachtel
    Proteome Center Tuebingen, University of Tuebingen, Tuebingen, Germany
    Mol Cell Proteomics 11:160-70. 2012
  7. pmc Concentric zones of active RhoA and Cdc42 around single cell wounds
    Hélène A Benink
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Biol 168:429-39. 2005
  8. pmc The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint
    Silke Hauf
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    J Cell Biol 161:281-94. 2003
  9. ncbi Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein
    M M Mogensen
    Department of Anatomy and Physiology, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 113:3013-23. 2000
  10. ncbi Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperature
    Günther Baravalle
    Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Wahringer Gurtel 18 20, 1090, Vienna, Austria
    Cell Tissue Res 320:99-113. 2005

Detail Information

Publications299 found, 100 shown here

  1. ncbi Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis
    M A Jordan
    Department of Biological Sciences, University of California, Santa Barbara 93106
    J Cell Sci 102:401-16. 1992
    ..In the present study, we characterized the effects of vinblastine, podophyllotoxin and nocodazole over broad concentration ranges on mitotic spindle organization in HeLa cells...
  2. pmc Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
    J Cell Biol 143:1505-21. 1998
    ..After nocodazole addition, time-lapse microscopy of GalNAc-T2-GFP and GalT-GFP revealed that scattered Golgi elements appeared ..
  3. ncbi Disorganized microtubules underlie the formation of retraction bulbs and the failure of axonal regeneration
    Ali Erturk
    Max Planck Institute of Neurobiology, Axonal Growth and Regeneration, 82152 Martinsried, Germany
    J Neurosci 27:9169-80. 2007
    ..Our data pinpoint microtubules as a key regulatory target for axonal regeneration...
  4. pmc Mitotic checkpoint slippage in humans occurs via cyclin B destruction in the presence of an active checkpoint
    Daniela A Brito
    Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, New York 12222, USA
    Curr Biol 16:1194-200. 2006
    ..fluorescence microscopy studies on rat kangaroo (PtK) and human (RPE1) cells dividing in the presence of nocodazole. We find that in the absence of microtubules (MTs), escape from mitosis occurs in the presence of an active SAC ..
  5. ncbi Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
    ..Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components...
  6. pmc Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells
    Mirita Franz-Wachtel
    Proteome Center Tuebingen, University of Tuebingen, Tuebingen, Germany
    Mol Cell Proteomics 11:160-70. 2012
    ..levels between constitutively active and kinase dead PKD1 strains of HEK293 cells, both treated with nocodazole, a microtubule-depolymerizing reagent that disrupts the Golgi complex and activates PKD1...
  7. pmc Concentric zones of active RhoA and Cdc42 around single cell wounds
    Hélène A Benink
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Biol 168:429-39. 2005
    ..Each of the zones makes distinct contributions to the organization and function of the actomyosin wound array. We propose that similar rho activity zones control related processes such as cytokinesis...
  8. pmc The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint
    Silke Hauf
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    J Cell Biol 161:281-94. 2003
    ..Hesperadin also causes cells arrested by taxol or monastrol to enter anaphase within <1 h, whereas cells in nocodazole stay arrested for 3-5 h...
  9. ncbi Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein
    M M Mogensen
    Department of Anatomy and Physiology, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH, UK
    J Cell Sci 113:3013-23. 2000
    ..We propose that ninein is a non-nucleating microtubule minus-end associated protein which may have a dual role as a minus-end capping and anchoring protein...
  10. ncbi Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperature
    Günther Baravalle
    Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Wahringer Gurtel 18 20, 1090, Vienna, Austria
    Cell Tissue Res 320:99-113. 2005
    The effects of bafilomycin, nocodazole, and reduced temperature on recycling and the lysosomal pathway have been investigated in various cultured cell lines and have been shown to vary dependent on the cell type examined...
  11. pmc The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint
    E Weiss
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder 80309 0347, USA
    J Cell Biol 132:111-23. 1996
    ..Arrest of mitosis upon microtubule depolymerization by nocodazole is also conditionally absent in mps1 strains...
  12. ncbi Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes
    Hayden A Homer
    Newcastle Fertility Centre at Life, International Centre for Life, Times Square, Newcastle upon Tyne, NE1 4EP, UK
    Reproduction 130:829-43. 2005
    ..As shown previously, we find that spindle depolymerisation by nocodazole inhibits first polar body extrusion (PBE) and stabilises cyclin B and cyclin-dependent kinase 1 activity in ..
  13. pmc A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint
    Daniel R Matson
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Genes Dev 26:542-7. 2012
    ..We propose a model to explain the differential response of human cells to nocodazole and paclitaxel.
  14. ncbi The small GTPase Cdc42 promotes membrane protrusion during polar body emission via ARP2-nucleated actin polymerization
    J Leblanc
    Ottawa Hospital Research Institute, Ottawa Hospital Civic Campus, Canada
    Mol Hum Reprod 17:305-16. 2011
    ..We report here that treating oocytes with low concentrations of nocodazole diminished the size of metaphase I spindles and prevented polar body emission, and yet an active Cdc42 cap of ..
  15. ncbi Myosin IIA regulates cell motility and actomyosin-microtubule crosstalk
    Sharona Even-Ram
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research NIDCR, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 9:299-309. 2007
    ..We conclude that myosin IIA negatively regulates cell migration and suggest that it maintains a balance between the actomyosin and microtubule systems by regulating microtubule dynamics...
  16. ncbi Disruption of haemocyte function by exposure to cytochalasin b or nocodazole increases the susceptibility of Galleria mellonella larvae to infection
    Nessa Banville
    Medical Mycology Unit, Department of Biology, NUI Maynooth, Co Kildare, Ireland
    Microbes Infect 13:1191-8. 2011
    Administration of non-toxic concentrations (10 μM) of cytochalasin b and nocodazole to larvae of Galleria mellonella increased their susceptibility to infection by the yeast Candida albicans...
  17. pmc Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution
    J K Burkhardt
    The University of Chicago, Department of Pathology, Chicago, Illinois 60637, USA
    J Cell Biol 139:469-84. 1997
    ..This redistribution was disrupted by nocodazole, implicating an underlying plus end-directed microtubule motor activity...
  18. pmc Dad1p, third component of the Duo1p/Dam1p complex involved in kinetochore function and mitotic spindle integrity
    M Enquist-Newman
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    Mol Biol Cell 12:2601-13. 2001
    ..In addition, dad1-1 mutants undergo chromosome mis-segregation at the restrictive temperature, resulting in a dramatic decrease in viability...
  19. pmc Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae
    Eva Maria Damm
    Institute of Biochemistry, Swiss Federal Institute of Technology Zurich ETHZ, CH 8093 Zurich, Switzerland
    J Cell Biol 168:477-88. 2005
    ..Our results revealed the existence of a virus-activated endocytic pathway from the plasma membrane to the ER that involves neither clathrin nor caveolae and that can be activated also in the presence of cav-1...
  20. ncbi The postsynaptic localization of the glycine receptor-associated protein gephyrin is regulated by the cytoskeleton
    J Kirsch
    Department of Neurochemistry, Max Planck Institute for Brain Research, Frankfurt Main, Federal Republic of Germany
    J Neurosci 15:4148-56. 1995
    ..These findings support the view that in vivo gephyrin anchors receptor polypeptides to the cytoskeleton by a complex interaction with microtubules and microfilaments...
  21. pmc Actin-dependent lamellipodia formation and microtubule-dependent tail retraction control-directed cell migration
    C Ballestrem
    Department of Pathology, Centre Medical Universitaire, Geneva, Switzerland
    Mol Biol Cell 11:2999-3012. 2000
    ..Treatment of cells with either the microtubule-disrupting agent nocodazole or with the stabilizing agent taxol showed decreased ruffling and lamellipodium formation...
  22. pmc The kinetics of force-induced cell reorganization depend on microtubules and actin
    Alexandra M Goldyn
    Department of New Materials and Biosystems, Max Planck Institute for Metals Research, Heisenbergstr 3, Stuttgart, Germany
    Cytoskeleton (Hoboken) 67:241-50. 2010
    ..Overall, we conclude that acto-myosin, together with microtubules, regulate the kinetics of force-induced cell reorientation...
  23. pmc Acetylated microtubules are required for fusion of autophagosomes with lysosomes
    Rui Xie
    Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, Texas A and M Health Science Center, Houston, Texas 77030 3303, USA
    BMC Cell Biol 11:89. 2010
    ....
  24. pmc Restraint of apoptosis during mitosis through interdomain phosphorylation of caspase-2
    Joshua L Andersen
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 28:3216-27. 2009
    ..Moreover, U2OS cells treated with nocodazole were found to undergo mitotic catastrophe more readily when endogenous caspase-2 was replaced with the S340A ..
  25. ncbi Inhibition of autophagy in mitotic animal cells
    Eeva Liisa Eskelinen
    Centre for High Resolution Imaging and Processing, MSI WTB complex, University of Dundee, School of Life Sciences, Dundee DD1 5EH, Scotland
    Traffic 3:878-93. 2002
    ..We found strong inhibition of autophagic vacuole accumulation in nocodazole-arrested pseudo-prometaphase cells, and also in metaphase and anaphase cells generated on release from the ..
  26. ncbi Synchronization of cell populations in G1/S and G2/M phases of the cell cycle
    Jane V Harper
    School of Animal and Microbial Sciences, University of Reading, Reading, UK
    Methods Mol Biol 296:157-66. 2005
    ..chapter utilizes a double thymidine block (an inhibitor of DNA synthesis) followed by treatment of cells with nocodazole (a mitotic inhibitor) to obtain large cell populations at distinct phases of the cell cycle...
  27. pmc The Golgi complex is a microtubule-organizing organelle
    K Chabin-Brion
    Laboratoire de Biochimie et de Biologie Cellulaire, EA 1595, , , France
    Mol Biol Cell 12:2047-60. 2001
    ..With the use of nocodazole wash-out experiments in hepatic cells, we found that the occurrence of noncentrosomal, early stabilized ..
  28. pmc Evidence that a defective spindle assembly checkpoint is not the primary cause of maternal age-associated aneuploidy in mouse eggs
    Francesca E Duncan
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biol Reprod 81:768-76. 2009
    ..When SAC function was challenged with a low concentration of the spindle toxin nocodazole, oocytes from both young and old mice arrested at meiosis I, which is indicative of a functional checkpoint...
  29. ncbi BubR1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte
    Liang Wei
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing, China
    Cell Cycle 9:1112-21. 2010
    ..Spindle disruption by nocodazole treatment caused relocation of BubR1 to kinetochores at metaphase I, anaphase I and metaphase II stages; spindle ..
  30. ncbi ER-to-Golgi transport visualized in living cells
    J F Presley
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    Nature 389:81-5. 1997
    ..This contrasts with the traditional focus on small vesicles as the primary vehicles for ER-to-Golgi transport...
  31. pmc Bub3 is a spindle assembly checkpoint protein regulating chromosome segregation during mouse oocyte meiosis
    Mo Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    PLoS ONE 4:e7701. 2009
    ..We propose that as a member of SAC, Bub3 is required for regulation of both meiosis I and II, and is potentially involved in kinetochore-microtubule attachment in mammalian oocytes...
  32. ncbi MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells
    L S Michel
    Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 409:355-9. 2001
    ..Furthermore, Mad2+/- mice develop lung tumours at high rates after long latencies, implicating defects in the mitotic checkpoint in tumorigenesis...
  33. pmc A novel role for the mitotic spindle during DNA segregation in yeast: promoting 2 microm plasmid-cohesin association
    Shwetal Mehta
    Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell Biol 25:4283-98. 2005
    ..In cells recovering from nocodazole-induced spindle depolymerization and G(2)/M arrest, cohesin-STB association can be established coincident with ..
  34. pmc Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae
    O W Rossanese
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
    J Cell Biol 145:69-81. 1999
    ..cerevisiae has a delocalized tER and therefore generates a dispersed Golgi. These findings open the way for a molecular genetic analysis of tER sites...
  35. pmc Exploring long-term protection of normal human fibroblasts and epithelial cells from chemotherapy in cell culture
    Pasha Apontes
    Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Oncotarget 2:222-33. 2011
    ..long-term cell recovery (rather than on a short-term cell survival) after a 3-day exposure to MI (paclitaxel and nocodazole)...
  36. pmc MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability
    Mei Leng
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:11485-90. 2006
    ..We propose that MPS1-dependent BLM phosphorylation is important for ensuring accurate chromosome segregation, and its deregulation may contribute to cancer...
  37. ncbi Metaphase I arrest upon activation of the Mad2-dependent spindle checkpoint in mouse oocytes
    Katja Wassmann
    Laboratoire de Biologie Cellulaire du Developpement, UMR7622, CNRS, Universite Pierre et Marie Curie, 9 quai Saint Bernard, 75005 Paris, France
    Curr Biol 13:1596-608. 2003
    ..On the contrary, the existence of such a checkpoint during the first meiotic division in mammalian oocytes when homologous chromosomes are segregated has remained controversial...
  38. ncbi Demecolcine- and nocodazole-induced enucleation in mouse and goat oocytes for the preparation of recipient cytoplasts in somatic cell nuclear transfer procedures
    Nuno Costa-Borges
    Departament de Biologia Cel lular, Fisiologia i Immunologia, Facultat de Biociencies, Universitat Autonoma de Barcelona, Spain
    Theriogenology 75:527-41. 2011
    ..Here, we explored whether short treatments with DEM or another antimitotic, nocodazole (NOC), improve IE efficiency, and determined the most appropriate timing for nuclear transfer in the cytoplasts ..
  39. ncbi Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronization
    Stephen Cooper
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    Cell Tissue Res 324:237-42. 2006
    It has been predicted that nocodazole-inhibited cells are not synchronized because nocodazole-arrested cells with a G2-phase amount of DNA would not have a narrow cell-size range reflecting the cell size of some specific, presumably G2-..
  40. pmc Transport of African swine fever virus from assembly sites to the plasma membrane is dependent on microtubules and conventional kinesin
    Nolwenn Jouvenet
    Department of Immunology and Pathology, Institute for Animal Health, Surrey GU24 0NF, United Kingdom
    J Virol 78:7990-8001. 2004
    ..Furthermore, virus movement to the cell periphery was inhibited when microtubules were depolymerized by nocodazole. In addition, ASFV infection resulted in the increased acetylation of microtubules as well as their protection ..
  41. ncbi Visualization of microtubule-mediated transport of influenza viral progeny ribonucleoprotein
    Fumitaka Momose
    Kitasato Institute for Life Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo 108 8641, Japan
    Microbes Infect 9:1422-33. 2007
    ..The treatment with nocodazole, a microtubule-depolymerizing agent, showed random distribution of the punctate NP in the cytoplasm...
  42. ncbi Nocodazole inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes via a microtubule-independent mechanism
    J C Molero
    Institute for Molecular Biosciences and the Department of Physiology and Pharmacology, University of Queensland, St Lucia, Queensland 4072, Australia
    J Biol Chem 276:43829-35. 2001
    ..Recent reports have shown that the microtubule-depolymerizing agent nocodazole inhibits insulin-stimulated glucose transport, implicating an important role for microtubules in this process...
  43. pmc Genetic and chemical modulation of spastin-dependent axon outgrowth in zebrafish embryos indicates a role for impaired microtubule dynamics in hereditary spastic paraplegia
    Richard Butler
    MRC Centre for Developmental and Biomedical Genetics, Department of Biomedical Science, University of Sheffield, Sheffield, S10 2TN, UK
    Dis Model Mech 3:743-51. 2010
    ..In addition, the microtubule-destabilising drug nocodazole abolished microtubule dynamics and growth cone motility, and enhanced phenotypic severity in spast-knockdown ..
  44. ncbi AuroraA overexpression overrides the mitotic spindle checkpoint triggered by nocodazole, a microtubule destabilizer
    Ying Jiang
    DNAX Research Inc, Palo Alto, CA 94304, USA
    Oncogene 22:8293-301. 2003
    ..overexpression and showed that increased AuroraA levels compromise the mitotic spindle checkpoint triggered by nocodazole, a microtubule polymerization inhibitor...
  45. ncbi Disruption of microtubules inhibits cytoplasmic ribonucleoprotein stress granule formation
    Pavel A Ivanov
    Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russian Federation
    Exp Cell Res 290:227-33. 2003
    ..Disruption of MT array with nocodazole treatment abolished arsenate-induced formation of stress granules...
  46. ncbi Length of mitotic arrest induced by microtubule-stabilizing drugs determines cell death after mitotic exit
    Michael E Bekier
    Department of Biological Sciences, University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA
    Mol Cancer Ther 8:1646-54. 2009
    ..Our experiments suggest that there is a point of no return during prolonged mitotic block after which mitotic exit can no longer block death...
  47. ncbi Cell cycle: stressed out of mitosis
    Alexei Mikhailov
    Division of Molecular Medicine, Wadsworth Center, New York State Dept of Health, Albany, NY 12201 0509, USA
    Curr Biol 12:R331-3. 2002
    ..Such stresses include the drug-induced disassembly of microtubules, which triggers an ATM-independent G2 checkpoint pathway involving a novel ubiquitin ligase...
  48. ncbi LPA-stimulated fibroblast contraction of floating collagen matrices does not require Rho kinase activity or retraction of fibroblast extensions
    David J Lee
    Department of Cell Biology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9039, USA
    Exp Cell Res 289:86-94. 2003
    ..Modulation was specific for LPA since fibroblast-collagen matrix contraction stimulated by platelet-derived growth factor was Rho kinase dependent before or after mechanical loading developed...
  49. pmc Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis
    P Kalitsis
    Murdoch Children s Research Institute, Royal Children s Hospital, Flemington Road, Parkville 3052, Melbourne, Australia
    Genes Dev 14:2277-82. 2000
    ..The results confirm Bub3 as a component of the essential spindle checkpoint pathway that operates during early embryogenesis...
  50. ncbi Giardia lamblia: evaluation of the in vitro effects of nocodazole and colchicine on trophozoites
    Rafael Meyer Mariante
    Programa de Ciências Morfológicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    Exp Parasitol 110:62-72. 2005
    ..In the present work, we used the microtubule inhibitors nocodazole and colchicine in order to investigate their direct and indirect effects on Giardia ultrastructure and ..
  51. pmc Cell cycle synchrony in Giardia intestinalis cultures achieved by using nocodazole and aphidicolin
    MARIANNE K POXLEITNER
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Eukaryot Cell 7:569-74. 2008
    ..The research presented here demonstrates reversible cell cycle arrest in G(1)/S and G(2)/M with aphidicolin and nocodazole, respectively...
  52. ncbi Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes
    Robert Köchl
    Cancer Research UK, London Research Institute, Secretory Pathways Laboratory, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Traffic 7:129-45. 2006
    ..To determine the role of microtubules in autophagy, we used nocodazole and vinblastine to disrupt microtubules and independently measured formation and fusion of autophagsosomes in ..
  53. pmc The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins
    R H Chen
    Department of Physiology, University of California, San Francisco, San Francisco, California 94143 0444, USA
    Mol Biol Cell 10:2607-18. 1999
    ..Deletion and mutational analysis of both proteins indicate that association of Mad2p with Mad1p is critical for checkpoint function and for hyperphosphorylation of Mad1p...
  54. pmc Overexpression of tau protein inhibits kinesin-dependent trafficking of vesicles, mitochondria, and endoplasmic reticulum: implications for Alzheimer's disease
    A Ebneth
    Max Planck Unit for Structural Molecular Biology, D 22607 Hamburg, Germany
    J Cell Biol 143:777-94. 1998
    ..Since in Alzheimer's disease tau protein is elevated and mislocalized, these observations point to a possible cause for the gradual degeneration of neurons...
  55. ncbi The power of chemotherapeutic engineering: arresting cell cycle and suppressing senescence to protect from mitotic inhibitors
    Mikhail V Blagosklonny
    Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY, USA
    Cell Cycle 10:2295-8. 2011
    ..By knowing the mechanisms of cell cycle arrest, death and senescence, we can design "rainbow combinations" that obediently kill or spare desired cells. Knowledge is power...
  56. pmc The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint
    S Biggins
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 15:3118-29. 2001
    ..Ipl1p localizes at or near kinetochores during mitosis, and we propose that Ipl1p is required to monitor tension at the kinetochore...
  57. pmc Brd4 marks select genes on mitotic chromatin and directs postmitotic transcription
    Anup Dey
    Laboratory of Molecular Growth Regulation, Program in Genomics of Differentiation, National Institute of Child Health and Human Development and Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 20:4899-909. 2009
    ..In sum, Brd4 marks M/G1 genes for transcriptional memory during mitosis, and upon exiting mitosis, this mark acts as a signal for initiating their prompt transcription in daughter cells...
  58. ncbi Dual roles of human BubR1, a mitotic checkpoint kinase, in the monitoring of chromosomal instability
    Hyun Jin Shin
    Research Institute, National Cancer Center, Goyang, Gyeonggi, 411 764, Korea
    Cancer Cell 4:483-97. 2003
    ..These results suggest that hBubR1-mediated apoptosis prevents the propagation of cells that breach the mitotic checkpoint and that the control of hBubR1 protein level is an important factor in the acquisition of preneoplastic polyploidy...
  59. pmc APC/C- and Mad2-mediated degradation of Cdc20 during spindle checkpoint activation
    Sheng Ge
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Cell Cycle 8:167-71. 2009
    ..We propose that the degradation of Cdc20 represents a critical control mechanism to ensure inactivation of APC/C(Cdc20) in response to the SAC...
  60. pmc Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint
    Q Liu
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 14:1448-59. 2000
    ..Taken together, these data indicate that Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression, and that Atr regulates Chk1...
  61. pmc Microtubule stabilization specifies initial neuronal polarization
    Harald Witte
    Axonal Growth and Regeneration, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany
    J Cell Biol 180:619-32. 2008
    ..Application of low doses of the microtubule-destabilizing drug nocodazole selectively reduces the formation of future dendrites...
  62. ncbi Cytoplasmic dynein as a facilitator of nuclear envelope breakdown
    Davide Salina
    Department of Cell Biology and Anatomy, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada
    Cell 108:97-107. 2002
    ..Support for this model is provided by the finding that interference with dynein function drastically alters nuclear membrane dynamics in prophase and prometaphase...
  63. ncbi Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria
    Y Tanaka
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Japan
    Cell 93:1147-58. 1998
    ..These data collectively indicate that kinesin is essential for mitochondrial and lysosomal dispersion rather than for the Golgi-to-ER traffic in these cells...
  64. ncbi Stuck in division or passing through: what happens when cells cannot satisfy the spindle assembly checkpoint
    Conly L Rieder
    Division of Molecular Medicine, New York State Department of Health, Wadsworth Center, Albany, NY 12201, USA
    Dev Cell 7:637-51. 2004
    ..This review defines and discusses the various factors that determine how long a cell remains in mitosis when it cannot satisfy the SAC and also discusses the cell's subsequent fate...
  65. pmc KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes
    Y Noda
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Cell Biol 155:77-88. 2001
    ..Absence of cytoplasmic dynein on these annexin XIIIb-associated vesicles and distinct distribution of the two motors on the EM level verified the existence of KIFC3-driven transport in epithelial cells...
  66. ncbi Bcl-2 phosphorylation and apoptosis activated by damaged microtubules require mTOR and are regulated by Akt
    Laura Asnaghi
    Department of Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy
    Oncogene 23:5781-91. 2004
    ..expressing Akt mutants, the level of Bcl-2 phosphorylation and the threshold of apoptosis induced by taxol or by nocodazole are significantly modified...
  67. pmc Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus
    B Sodeik
    Yale University School of Medicine, Department of Cell Biology, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 136:1007-21. 1997
    ..We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus...
  68. ncbi Nocodazole, a microtubule de-polymerising agent, induces apoptosis of chronic lymphocytic leukaemia cells associated with changes in Bcl-2 phosphorylation and expression
    Richard W Beswick
    Division of Investigative Sciences, Department of Haematology, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Leuk Res 30:427-36. 2006
    ..this study, we investigate in detail the specificity and mechanism by which a microtubule de-polymerising agent, nocodazole, induces apoptosis in non-cyclingm, i.e. G(0)/G(1), chronic lymphocytic leukaemia (CLL) B-cells...
  69. ncbi Kv1.5 surface expression is modulated by retrograde trafficking of newly endocytosed channels by the dynein motor
    Woo Sung Choi
    Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    Circ Res 97:363-71. 2005
    ..01), as did treatment of the cells with a dynamin inhibitory peptide, which blocks endocytosis. Nocodazole pretreatment, which depolymerizes the microtubule cytoskeleton along which dynein tracks, also doubled Kv1...
  70. pmc Golgi spectrin: identification of an erythroid beta-spectrin homolog associated with the Golgi complex
    K A Beck
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305 5426
    J Cell Biol 127:707-23. 1994
    ..In contrast, perturbation of Golgi structure without a loss of function, by the addition of nocodazole, results in retention of beta-spectrin with the dispersed Golgi elements...
  71. pmc Brd4 is required for recovery from antimicrotubule drug-induced mitotic arrest: preservation of acetylated chromatin
    Akira Nishiyama
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Mol Biol Cell 17:814-23. 2006
    ..was rapidly released from chromosomes upon treatment with antimicrotubule drugs, including the reversible agent nocodazole. Yet, when nocodazole was withdrawn, Brd4 was reloaded onto chromosomes, and cells proceeded to complete cell ..
  72. ncbi Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1
    D Y Jin
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892 0460, USA
    Cell 93:81-91. 1998
    ..conservation with yeast MAD1, hyperphosphorylation during S/G2/M phases and upon treatment of cells with nocodazole, and binding to HsMAD2. HsMAD1 functions as a homodimer...
  73. pmc Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells
    D Cimini
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Cell Biol 153:517-27. 2001
    ..55% after a mitotic block with 2 microM nocodazole. A lagging chromosome seen during anaphase in control or nocodazole-treated cells was found by confocal ..
  74. pmc G(1) and G(2) cell-cycle arrest following microtubule depolymerization in human breast cancer cells
    April L Blajeski
    Department of Molecular Pharmacology and Experimental Therapeutics, Tumor Biology Program, Mayo Graduate School, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    J Clin Invest 110:91-9. 2002
    ..After treatment with 1 microM nocodazole, seven of ten breast cancer lines (type A cells) arrested in mitosis, whereas the other three (type B cells) did ..
  75. ncbi Activation of the protein kinase p38 in the spindle assembly checkpoint and mitotic arrest
    K Takenaka
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 01, Japan
    Science 280:599-602. 1998
    ..activated in mammalian cultured cells when the cells were arrested in M phase by disruption of the spindle with nocodazole. Addition of activated recombinant p38 to Xenopus cell-free extracts caused arrest of the extracts in M phase, ..
  76. ncbi Imaging macrophage chemotaxis in vivo: studies of microtubule function in zebrafish wound inflammation
    Michael J Redd
    Cell Motility Group, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, London, United Kingdom
    Cell Motil Cytoskeleton 63:415-22. 2006
    ..Using this assay, we test the role of the microtubule cytoskeleton in macrophage chemotaxis in vivo using nocodazole to disrupt microtubule polymerization...
  77. ncbi S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function
    M A Hoyt
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218
    Cell 66:507-17. 1991
    ..Our studies have defined three genes required for normal cell cycle arrest. These findings are consistent with the existence of a surveillance system that halts the cell cycle in response to microtubule perturbation...
  78. ncbi Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes
    Masahiro Kawakami
    Laboratory of Animal Reproduction, College of Agriculture, Kinki University, 3327 204, Nakamachi, Nara 631 8505, Japan
    Cloning Stem Cells 5:379-87. 2003
    ..The present study examined the optimal conditions for demecolcine and nocodazole treatment in chemically assisted removal of chromosomes. When matured oocytes were treated with 0.1-0...
  79. ncbi Mutations of mitotic checkpoint genes in human cancers
    D P Cahill
    The Johns Hopkins Oncology Center, Program in Human Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Nature 392:300-3. 1998
    ..The normal mitotic checkpoints of cells displaying microsatellite instability become defective upon transfer of mutant hBUB1 alleles from either of two CIN cancers...
  80. pmc The golgin tether giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus
    Mayuko Koreishi
    The Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
    PLoS ONE 8:e59821. 2013
    ..Treatment with the microtubule destabilizing drug nocodazole transforms the Golgi ribbon into individual Golgi stacks...
  81. ncbi Rethinking synchronization of mammalian cells for cell cycle analysis
    S Cooper
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Cell Mol Life Sci 60:1099-106. 2003
    ..Release of arrested cells from inhibition does not produce cells reflecting cells during the normal division cycle. Thus, cells produced by batch or forcing methods are not experimental models for analysis of the normal cell cycle...
  82. pmc Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro
    R J Vasquez
    Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA
    Mol Biol Cell 8:973-85. 1997
    Previous studies demonstrated that nanomolar concentrations of nocodazole can block cells in mitosis without net microtubule disassembly and resulted in the hypothesis that this block was due to a nocodazole-induced stabilization of ..
  83. pmc Checkpoint genes required to delay cell division in response to nocodazole respond to impaired kinetochore function in the yeast Saccharomyces cerevisiae
    Y Wang
    Department of Biology, University of Virginia, Charlottesville 22903, USA
    Mol Cell Biol 15:6838-44. 1995
    ..We show that low concentrations of nocodazole delay cell division under the control of the previously identified mitotic checkpoint genes BUB1, BUB3, MAD1, ..
  84. pmc Modulation of substrate adhesion dynamics via microtubule targeting requires kinesin-1
    Olga Krylyshkina
    Institute of Molecular Biology, Austrian Academy of Sciences, Billrothsthstrasse 11, Salzburg 5020, Austria
    J Cell Biol 156:349-59. 2002
    ..and reduction in number of substrate adhesions, mimicking the effect observed after microtubule disruption by nocodazole. Blockage of kinesin activity had no influence on either the ability of microtubules to target substrate ..
  85. ncbi Microtubule-targeted drugs inhibit VEGF receptor-2 expression by both transcriptional and post-transcriptional mechanisms
    Markus Meissner
    Department of Dermatology, Klinikum der Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    J Invest Dermatol 128:2084-91. 2008
    ..Hence, both inhibition of the rate of gene transcription and increased mRNA turnover represent critical molecular mechanisms by which MT disruption inhibits VEGFR2 expression...
  86. ncbi Phosphorylation of STAT3 serine-727 by cyclin-dependent kinase 1 is critical for nocodazole-induced mitotic arrest
    Xiaoqing Shi
    Brain Research Center and Department of Medicine, University of British Columbia, Vancouver, Canada
    Biochemistry 45:5857-67. 2006
    ..Kinetworks KPSS-1.1 phospho-site screening of nocodazole-treated HeLa cells revealed that STAT3 Ser-727 phosphorylation was enhanced during mitosis, and this correlated ..
  87. pmc The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis
    P Collas
    Institute of Medical Biochemistry, Faculty of Medicine, University of Oslo, Blindern, 0317 Oslo, Norway
    J Cell Biol 147:1167-80. 1999
    ..We propose that AKAP95 is a multivalent molecule that in addition to anchoring a cAMP/PKA-signaling complex onto chromosomes, plays a role in regulating chromosome structure at mitosis...
  88. ncbi Raf-1/MEK/MAPK pathway is necessary for the G2/M transition induced by nocodazole
    C Hayne
    Diabetes and Metabolism Research Unit, Section of Endocrinology, Evans Department of Medicine and the Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 275:31876-82. 2000
    ..for cells to enter and exit mitosis, and drugs that disrupt this balance, such as taxol, colchicine, and nocodazole, arrest the cell cycle in mitosis...
  89. ncbi Localization of Tctex-1, a cytoplasmic dynein light chain, to the Golgi apparatus and evidence for dynein complex heterogeneity
    A W Tai
    Department of Cell Biology and Anatomy, Margaret M Dyson Vision Research Institute, Cornell University Medical College, New York, New York 10021, USA
    J Biol Chem 273:19639-49. 1998
    ..Distinct populations of dynein complexes are likely to exist, and such diversity may occur in part at the level of their light chain compositions...
  90. pmc Nocodazole treatment decreases expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells
    Ade Kallas
    Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
    PLoS ONE 6:e19114. 2011
    b>Nocodazole is a known destabiliser of microtubule dynamics and arrests cell-cycle at the G2/M phase. In the context of the human embryonic stem cell (hESC) it is important to understand how this arrest influences the pluripotency of cells...
  91. pmc Role of cyclin B1/Cdc2 up-regulation in the development of mitotic prometaphase arrest in human breast cancer cells treated with nocodazole
    Hye Joung Choi
    Department of Pharmacology, Toxicology and Therapeutics, School of Medicine, University of Kansas Medical Center, Kansas City, Kansas, United States of America
    PLoS ONE 6:e24312. 2011
    ..Here we report our finding that treatment of MCF-7 human breast cancer cells with nocodazole, a prototypic microtubule inhibitor, results in strong up-regulation of cyclin B1 and Cdc2 levels, and their ..
  92. ncbi Centrosome-dependent exit of cytokinesis in animal cells
    M Piel
    Institut Curie, Section Recherche, UMR 144 du CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France
    Science 291:1550-3. 2001
    ....
  93. ncbi Regulation of microtubule stability by the von Hippel-Lindau tumour suppressor protein pVHL
    Alexander Hergovich
    Friedrich Miescher Institut, Maulbeerstrasse 66, Basel CH 4058, Switzerland
    Nat Cell Biol 5:64-70. 2003
    ..Our data identify a role for pVHL in the regulation of microtubule dynamics and potentially provide a link between this function of pVHL and the pathogenesis of haemangioblastoma and phaeochromocytoma in the context of VHL disease...
  94. ncbi A Golgi PKD activity reporter reveals a crucial role of PKD in nocodazole-induced Golgi dispersal
    Yannick F Fuchs
    Institute of Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Traffic 10:858-67. 2009
    ..Interestingly, nocodazole-induced Golgi complex break-up and dispersal was associated with local PKD activation as measured by reporter ..
  95. ncbi Nocodazole-induced changes in microtubule dynamics impair the morphology and directionality of migrating medial ganglionic eminence cells
    Jean Pierre Baudoin
    INSERM, U839, Hopital Pitie Salpetriere, Paris, France
    Dev Neurosci 30:132-43. 2008
    ..A low (100 nM) concentration of nocodazole shortened the leading process of MGE cells that nevertheless continued to migrate at the same rate but ..
  96. ncbi Effects of microtubule disruption on force, velocity, stiffness and [Ca(2+)](i) in porcine coronary arteries
    R J Paul
    Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA
    Am J Physiol Heart Circ Physiol 279:H2493-501. 2000
    ..In porcine coronary arteries, the disruption of microtubules by nocodazole (11 microM) also elicited moderate but significant increases in isometric force (10-40% of a KCl contracture), ..
  97. ncbi Metaphase I arrest in LT/Sv mouse oocytes involves the spindle assembly checkpoint
    Anna Hupalowska
    Department of Embryology, Institute of Zoology, Faculty of Biology, University of Warsaw, 02 096 Warsaw, Poland
    Biol Reprod 79:1102-10. 2008
    ..These data show for the first time that there is a direct link between the SAC function and the heritable meiotic incompetence of a mammalian oocyte...
  98. ncbi Cytoskeletal and morphological alterations underlying axonal sprouting after localized transection of cortical neuron axons in vitro
    Jyoti A Chuckowree
    Discipline of Pathology, Faculty of Health Sciences, University of Tasmania, Hobart, Tasmania, 7000, Australia
    J Neurosci 23:3715-25. 2003
    ..Exposure to the microtubule destabilizing agent nocodazole (100 microg/ml) resulted in substantial morphological alteration of developing neurons and inhibited neurite ..
  99. ncbi A quartz crystal microbalance cell biosensor: detection of microtubule alterations in living cells at nM nocodazole concentrations
    K A Marx
    Department of Chemistry, Center for Intelligent Biomaterials, University of Massachusetts, Lowell, MA 01854, USA
    Biosens Bioelectron 16:773-82. 2001
    ..We then utilized this EC QCM biosensor for the detection of the effect of [nocodazole] on the steady state Deltaf and DeltaR shift values...
  100. ncbi Dynamics of the interphase mammalian Golgi complex as revealed through drugs producing reversible Golgi disassembly
    B Storrie
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0308, USA
    Biochim Biophys Acta 1404:127-37. 1998
    We focus on research aimed at understanding normal Golgi complex dynamics through the use of nocodazole and other drugs which cause Golgi disassembly...
  101. ncbi Overexpression of DRG2 increases G2/M phase cells and decreases sensitivity to nocodazole-induced apoptosis
    Hebok Song
    Immunomodulation Research Center, University of Ulsan, Ulsan 680 749, Korea
    J Biochem 135:331-5. 2004
    ..In addition, an overexpression of DRG2 reduced the sensitivity of Jurkat cells to the mitotic poison nocodazole. Our data suggest that overexpression of DRG2 in Jurkat cells affects genes regulating cell-cycle arrest and ..

Research Grants62

  1. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..of -Syn, causing NET function and trafficking to be dysregulated and unresponsive to the effects of nocodazole [a MT destabilizing agent], probably due to tight binding of the -Syn/NET complex to the MT cytoskeleton...
  2. Imaging retinal astrocytes, ganglion cells and axonal transport in vivo
    Brad Fortune; Fiscal Year: 2012
    ..methods by comparing the impact of four unilateral experimental injury models (intravitreal injection of nocodazole/colchicine to disrupt axonal microtubules and inhibit active axonal transport;acute elevation of intraocular ..
  3. MECHANISMS OF SYNERGISTIC REGULATION OF STEM/PROGENITORS
    Hal E Broxmeyer; Fiscal Year: 2013
    ..b) Elucidate influence in vitro of Sirt1 on different stresses (genotoxic: nocodazole, paclitaxel, etoposide;oxidative: hypoxia and ROS;apoptosis: cytokine withdrawal and irradiation) to Sirt 1 -/-, ..
  4. Engineering of Human Corneal Endothelial Grafts
    Ying Ting Zhu; Fiscal Year: 2012
    ..of p120- catenin siRNA knockdown followed by additional Kaiso siRNA knockdown (Aim 1), and by addition of nocodazole to enhance p120-catenin nuclear translocation (Aim 2)...
  5. Enteropathogenic E. coli effector proteins in intestinal tight junction recovery
    LILA GOLLOGLY; Fiscal Year: 2013
    ..IEC will be transfected to express EspG1, or treated with nocodazole to disrupt MTs. Effects on TJ recovery will be determined...
  6. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey L Khodjakov; Fiscal Year: 2013
    ..2) treatment with low concentration of nocodazole, a condition known to prevent satisfaction of the SAC despite highly-stretched centromeres...
  7. Identification of key hematopoietic fate regulators induced by Notch signaling
    LEIGH ANN AKI HIGA; Fiscal Year: 2010
    ..Spindle damage will be induced with the microtubule depolymerizing drug nocodazole, and then we will analyze the recovery from spindle checkpoint arrest by light microscopy when the drug is ..
  8. The Biochemical Basis for the Mechanics of Cytokinesis
    Douglas N Robinson; Fiscal Year: 2012
    ..We will study 14-3-3, which was identified as a suppressor of nocodazole. Genetic interactions between RacE and 14-3-3 further point towards a pathway in which microtubules regulate ..
  9. FORMATION,INTERACTION & FUNCTION OF SPINDLE COMPONENTS
    Conly L Rieder; Fiscal Year: 2010
    ..microsurgery and GFP-imagingto test the hypotheses: a) that the mitotic arrest induced in vertebrate cells by nocodazole or Taxol is due to kinetochores;b) that C-anaphase in these cells requires a functional centrosome;c) that C-..
  10. Role of the Acetyltransferase p300 in Cellular Responses
    Maria Avantaggiati; Fiscal Year: 2006
    ..drugs of clinical relevance include paclitaxel (taxol), vinca alkaloids (vinblastin and vincrisitn), nocodazole and colchicine...
  11. MOLECULAR BIOLOGY OF INFECTION WITH M TUBERCULOSIS
    Lee Riley; Fiscal Year: 2002
    ..Reagents that affect cytoskeletal rearrangement (cytochalasin D, nocodazole, and onodansylcadaverine) inhibited the association with HeLa cells of microspheres coated with Mcep1...
  12. Live Cell Imaging of Rapidly Induced Golgi Unstacking and its Effects on Intra-Go
    Myun Hwa Dunlop; Fiscal Year: 2013
    ..The unstacking of nocodazole-induced peripheral Golgi mini-stacks will be monitored by triple color total internal reflection fluorescence (..
  13. Alpha- & Gamma-Synuclein Modulation of the NET After Antidepressant Treatment of
    Alexis Jeannotte; Fiscal Year: 2007
    ..a-Syn modulation of NET is fully reversed by nocodazole, a microtubule (MT) destabilizing agent, while ?-Syn regulation of NET is insensitive to nocodazole...
  14. THE PIGMENT MOTILITY MECHANISM IN CHROMATOPHORES
    Keith Porter; Fiscal Year: 1980
    ..directed motion of pigment has been demonstrated by partially disassembling the microtubules with colchicine and nocodazole. In their absence the motility mechanism cannot function...
  15. PPK: Radiation Response and Cell Proliferation
    Peter Stambrook; Fiscal Year: 2006
    ..of a role in cell cycle arrest or DNA repair, and it becomes phosphorylated following exposure of cells to nocodazole, suggestive of a role in the spindle assembly checkpoint...
  16. Cancer Biology and Chemical Genetics in the Zebrafish
    Howard Stern; Fiscal Year: 2004
    ..To further characterize the cell cycle defects, we will utilize chemicals (e.g., nocodazole) to synchronize the cell cycle in wild-type and mutant embryos...
  17. PHOSPHOLIPASE C AND KINETOCHORE FUNCTION
    Ales Vancura; Fiscal Year: 2001
    ..We found that cells with deletion of PLC1 gene (plc1delta) display higher frequency of chromosome loss, nocodazole sensitivity, and mitotic delay...
  18. CORTICAL ORGANIZATION IN PREIMPLANTATION MOUSE EMBRYOS
    JAEL SOBEL; Fiscal Year: 1990
    ..Correlative studies with the cytoskeletal inhibitors nocodazole and cytochalasin D are designed to elucidate the function of the filaments...
  19. ILK-Actopaxin Interactions in Cell Signaling
    Christopher E Turner; Fiscal Year: 2010
    ..fibroblasts to assess effect on cell polarity and focal adhesion disassembly utilizing potassium depletion and nocodazole washout assays respectively...
  20. HDAC4 Viability: Unique Mechanisms and Function
    Gary Kao; Fiscal Year: 2007
    ..inhibitor blocked phosphorylation of BubR1 and histone H3, and negated mitotic arrest after exposure to nocodazole. We believe these effects of HDAC4 are mechanistically linked and together influence cancer cell viability...
  21. MONOCYTE/MACROPHAGE SUBCELLULAR LIPOPROTEIN PROCESSING
    Nancy Jones; Fiscal Year: 2002
    ..and dextrans Additionally macropinocytosis and endocytosis will be functionally differentiated by wortmannin, nocodazole, hypertonic medium, and cytoplasmic acidification...
  22. The role of Cdc27 in miotic regulation of the APC/C
    Dina Matheos; Fiscal Year: 2005
    ..revealed a c-terminal deletion of Cdc27 that prematurely separates its sister chromatids in the presence of nocodazole. To extend these observations, I propose to use both genetic and biochemical approaches in the budding yeast ..
  23. Insulin Regulation of Adipocyte Microtubules
    ANN OLSON; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  24. Mechanisms of Regulation of GLUT4 Expression
    ANN OLSON; Fiscal Year: 2005
    ..abstract_text> ..
  25. Mechanisms of the HBX-interacting protein (HBXIP)
    John Reed; Fiscal Year: 2008
    ..Altogether, these investigations will define the role of HBXIP in regulating cell division and apoptosis, as well as providing new insights into the mechanisms of HBx in the pathogenesis of HCC. ..
  26. Chemical Inhibitors of ER Stress
    John Reed; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  27. Chemical Antagonists of IAP-Family Anti-Apoptotic Proteins
    John Reed; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  28. PREVENTION OF COLORECTAL CANCER BY iNOS AND COX-2 SELECTIVE INHIBITORS
    CHINTHALAPALLY RAO; Fiscal Year: 2008
    ..abstract_text> ..
  29. RNA Trafficking in Neural Cells.
    John Carson; Fiscal Year: 2009
    ..The results may reveal potential new targets for clinical intervention in neurological disease. ..
  30. INTERMEDIATE FILAMENT CELL SURFACE INTERACTIONS
    Robert Goldman; Fiscal Year: 2008
    ..The latter include the formation of Mallory bodies in alcoholic liver disease and Lewy bodies in Parkinson's disease. ..
  31. Mitochondria and Apoptosis (revised GM60554)
    John Reed; Fiscal Year: 2009
    ..abstract_text> ..
  32. BI-1, A Regulator of ER-Stress Pathways Linked to Apoptosis
    John Reed; Fiscal Year: 2009
    ..Altogether, these studies will improve understanding of how damage to the ER is linked to apoptosis, and will potentially reveal strategies for neuronal preservation in stroke and neurodegenerative diseases. ..
  33. HTS Assay for UBC13, a Mediator of Inflammatory Signaling
    John Reed; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  34. Regulation of Microdomain Structure in Living Cells
    Anne K Kenworthy; Fiscal Year: 2010
    ..These studies will also contribute to our long- term goal of understanding how membrane structure regulates cellular functions. ..
  35. Cytokinesis and the Cytoskeleton in C. elegans Embryos
    BRUCE A BOWERMAN; Fiscal Year: 2010
    ..Matthias Peter at the ETH in Zurich, Switzerland. The processes and genes we propose to investigate are relevant to important human diseases, including cancer and neurodegenerative disease. ..
  36. Cilial dysfunction and pathogenesis of obesity
    Bradley K Yoder; Fiscal Year: 2010
    ....
  37. Gene networks specifying cell lineages in a polychaete
    Bruce Bowerman; Fiscal Year: 2007
    ..Newly discovered and conserved loci will also be studied in C. elegans and Drosophila. [unreadable] [unreadable]..
  38. PARACRINE REGULATION OF BILE DUCT SECRETION BY ATP
    JONATHAN DRANOFF; Fiscal Year: 2004
    ..The Yale School of Medicine Department of Internal Medicine has pledged that the candidate will have extensive protected research time and excellent resources for the duration of the award. ..
  39. CED-4 FAMILY PROTEINS
    John Reed; Fiscal Year: 2003
    ..The information provided by these investigations may provide insights into human diseases where dysregulation of programmed cell death is known to occur and may lead to new strategies for therapeutic intervention. ..
  40. TRAINING IN SMOOTH AND CARDIAC MUSCLE BIOLOGY
    Richard Paul; Fiscal Year: 2003
    ..This arrangement promotes scientific interactions and enhance the breadth of educational and research experiences in our cardiovascular training program. ..
  41. CELLUAR MECHANISMS OF NEURONAL MORPHOGENESIS
    Mark Black; Fiscal Year: 2003
    ..By defining normal mechanisms for generating and maintaining microtubule arrangements in neurons, the proposed research will contribute toward a better understanding of these pathologies. ..
  42. MOLECULAR MECHANISMS OF HEDGEHOG SIGNALING
    Elizabeth Sztul; Fiscal Year: 2003
    ..Using Drosophila, new components of Hh signaling will be identified by a genetic screen involving a specific ptc phenotype. ..
  43. TG737 SIGNALING PATHWAY AND POLYCYSTIC KIDNEY
    Bradley Yoder; Fiscal Year: 2003
    ..Overall, results generated from this proposal will give important insight into the biological function of Tg737 and how disruption of the Tg737/polycystin-1 signaling pathway results in the formation of renal cyst. ..
  44. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..
  45. Apoptosis and transport mechanisms in neurodegeneration
    David DeWitt; Fiscal Year: 2002
    ..Cytoskeletal abnormalities including tau phosphorylation, neurofilament accumulation, and microtubule disruption will be examined to determine whether these changes. ..
  46. INTERACTION OF ORAL STREPTOCOCCI WITH ANIMAL TISSUES
    MURRAY STINSON; Fiscal Year: 2001
    ..These experiments will determine whether these streptococcal components act as independent or synergistic modulins. ..
  47. Dyslipidemia and retinal endothelial cell dysfunction
    Julia Busik; Fiscal Year: 2004
    ..The outcome of this work will lead to a better understanding of how diabetic dyslipidemia contributes to altered hRVE cell phenotype and the onset and progression of diabetic retinopathy. ..
  48. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..The knowledge will then be applied to the question of how the deregulation of the checkpoint function may contribute to cancer progression. ..
  49. INTESTINAL ADENOSINE A2B RECEPTOR
    Shanthi Sitaraman; Fiscal Year: 2004
    ..Third, I will study the role of adenosine receptor in the modulation and feedback regulation of neutrophil-epithelial interaction including transmigration and chemokine secretion. ..
  50. Resolving the nuclear fusion steps during yeast mating
    Patricia Melloy; Fiscal Year: 2006
    ..This information and the tools used can then be applied to other cell biological and gene regulation questions during other types of membrane fusion. ..
  51. Olympus FV1000 Laser Scanning Confocal Microscope
    William Bement; Fiscal Year: 2007
    ..This information, in turn, will help guide future investigations of a number of normal and pathological human processes such as wound repair, nerve regeneration, cancer, and birth defects. [unreadable] [unreadable] [unreadable]..
  52. Conserved Mechanisms of Ciliogenesis
    Bradley Yoder; Fiscal Year: 2007
    ..abstract_text> ..
  53. FLUORESCENT SPECKLE MICROSCOPY
    EDWARD SALMON; Fiscal Year: 2007
    ..Experience gained in the course of these studies will be used to direct and refine hardware and software development. [unreadable] [unreadable]..
  54. MECHANISMS OF MICROTUBULE AND MITOTIC SPINDLE ASSEMBLY
    Lynne Cassimeris; Fiscal Year: 2006
    ..To address these issues, we will apply a number of fluorescence-based imaging methods combined with siRNA or injection of function-blocking proteins or antibodies. ..
  55. ASCB Summer Meeting: Coordinating the Events of Directed
    Clare Waterman Storer; Fiscal Year: 2005
    ..A more thorough understanding of the processes involved will impact on multiple health-related issues, such as cancer treatment, wound recovery, and anti-inflammatory therapy. ..
  56. Gap Junction Assembly: Mechanisms and Regulation
    ROSS JOHNSON; Fiscal Year: 2006
    ..Finally, "pull-down" experiments will identify proteins associating with Cx43, in response to cAMP, and various biochemical techniques will be used to study wild type and mutant Cx43 in fibroblasts. ..
  57. Polyamines and Breast Cancer Biology
    Andrea Manni; Fiscal Year: 2005
    ..The ultimate goal of these studies is to identify critical signaling molecules which can be targeted in conjunction with antipolyamine therapy to optimize prevention of metastasis from breast cancer. ..
  58. Cell Biology of Human Thiamine Transporters
    VEEDAMALI SUBRAMANIAN; Fiscal Year: 2005
    ....
  59. Protein Interactions Involved in Poxvirus Envelopment
    Brian Ward; Fiscal Year: 2005
    ....
  60. POLYAMINE INVOLVEMENT IN MAMMARY CARCINOGENESIS
    Andrea Manni; Fiscal Year: 2001
    ....