Genomes and Genes
Summary: An enzyme catalyzing the transfer of a phosphate group from 3-phospho-D-glycerate in the presence of ATP to yield 3-phospho-D-glyceroyl phosphate and ADP. EC 22.214.171.124.
Publications287 found, 100 shown here
- The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent miceCyntia G M C Trant
Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil
Infect Immun 78:2283-91. 2010..Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308...
- Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheatShaoxing Huang
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 99:8133-8. 2002..We analyzed Acc-1 (plastid acetyl-CoA carboxylase) and Pgk-1 (plastid 3-phosphoglycerate kinase) genes to determine phylogenetic relationships among Triticum and Aegilops species of the wheat lineage ..
- Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysisMatthew J Cliff
The Krebs Institute and The Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
J Am Chem Soc 132:6507-16. 2010Transition state analogue (TSA) complexes formed by phosphoglycerate kinase (PGK) have been used to test the hypothesis that balancing of charge within the transition state dominates enzyme-catalyzed phosphoryl transfer...
- Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinaseJudit Szabo
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, PO Box 7, Hungary, Germany
Biochemistry 47:6735-44. 20083-Phosphoglycerate kinase is a hinge-bending enzyme with substrate-assisted domain closure. However, the closure mechanism has not been described in terms of structural details...
- Physiological promoters reduce the genotoxic risk of integrating gene vectorsDaniela Zychlinski
Department of Experimental Hematology, Hannover Medical School, Hannover, Germany
Mol Ther 16:718-25. 2008..promoters than did cellular promoters derived from human genes, elongation factor-1alpha (EF1alpha) and phosphoglycerate kinase (PGK). Self-inactivating (SIN) vector design reduced but did not abolish enhancer interactions...
- The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cellsJ K Vishwanatha
Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
J Cell Sci 101:25-34. 1992..have previously identified the protein-tyrosine kinase substrate annexin II, and the glycolytic enzyme 3-phosphoglycerate kinase as components of PRP...
- Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progressionJianhua Wang
Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China
Cancer Res 70:471-80. 2010..microdissection and cDNA microarray analysis of CAFs in prostate tumors revealed strong upregulation of phosphoglycerate kinase-1 (PGK1), an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway and is directly ..
- Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3'UTRMingang Xu
Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104 6080, USA
Biol Reprod 76:1025-33. 2007The mRNA that encodes the testis-specific protein phosphoglycerate kinase (PGK2) is a long-lived mRNA that is transcribed in meiotic and postmeiotic male germ cells...
- Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowdingApratim Dhar
Department of Chemistry, University of Illinois, Urbana, IL 61801, USA
Proc Natl Acad Sci U S A 107:17586-91. 2010..show how macromolecular crowding dramatically affects the structure, function, and folding landscape of phosphoglycerate kinase (PGK)...
- A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinaseLouiza Zerrad
Structural Biology Group, European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F 38043 Grenoble, France
J Biol Chem 286:14040-8. 2011b>Phosphoglycerate kinase (PGK) is the enzyme responsible for the first ATP-generating step of glycolysis and has been implicated extensively in oncogenesis and its development...
- Molecular basis for the lack of enantioselectivity of human 3-phosphoglycerate kinaseC Gondeau
Centre d Etudes d Agents Pathogenes et Biotechnologies pour la Sante, UMR 5236, CNRS Universités Montpellier 1 et 2, Institut de Biologie, 4 Bd Henri IV, CS69033, 34965 Montpellier cedex 2, France
Nucleic Acids Res 36:3620-9. 2008..to reveal the molecular basis for the low enantioselectivity and the broad specificity of human 3-phosphoglycerate kinase (hPGK), an enzyme responsible for the last step of phosphorylation of many nucleotide derivatives...
- Promoter-dependent EGFP expression during embryonic stem cell propagation and differentiationRong Wang
Key Laboratory of Stem Cell Biology of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Stem Cells Dev 17:279-89. 2008..the transcriptional activities of three ubiquitous promoters, elongation factor-1alpha (EF1alpha), phosphoglycerate kinase-1 (PGK), and cytomegalovirus (CMV), during propagation and differentiation of mouse (m) ES cells by using ..
- Binding of group B streptococcal phosphoglycerate kinase to plasminogen and actinTyler J Boone
The Department of Laboratory Medicine and Pathology, The University of Alberta, Edmonton, Alberta, Canada
Microb Pathog 51:255-61. 2011The glycolytic enzyme, phosphoglycerate kinase (PGK) of group B streptococci (GBS), has previously been identified as expressed on the GBS cell surface...
- Crystal structure of Plasmodium falciparum phosphoglycerate kinase: evidence for anion binding in the basic patchCraig D Smith
University of Alabama at Birmingham, Birmingham, AL 35294, USA
Biochem Biophys Res Commun 412:203-6. 20113-Phosphoglycerate kinase (EC 126.96.36.199) is a key enzyme in the glycolytic pathway and catalyzes an important phosphorylation step leading to the production of ATP...
- Evolutionary dynamics of the Pgk1 gene in the polyploid genus Kengyilia (Triticeae: Poaceae) and its diploid relativesXing Fan
Triticeae Research Institute, Sichuan Agricultural University, Sichuan, People s Republic of China
PLoS ONE 7:e31122. 2012....
- Phosphoglycerate kinase 1 promoting tumor progression and metastasis in gastric cancer - detected in a tumor mouse model using positron emission tomography/magnetic resonance imagingDerek Zieker
Department of General, Visceral and Transplant Surgery, Comprehensive Cancer Center, University of Tuebingen, Tuebingen, Germany
Cell Physiol Biochem 26:147-54. 2010..In previous studies we were able to show a relevant impact of increased phosphoglycerate kinase 1 expression (PGK1; a glycolytic enzyme) on invasive properties of gastric cancer in-vivo and in-vitro...
- Phosphoglycerate kinase and triosephosphate isomerase from the hyperthermophilic bacterium Thermotoga maritima form a covalent bifunctional enzyme complexH Schurig
Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, Germany
EMBO J 14:442-51. 1995b>Phosphoglycerate kinase (PGK) from the hyperthermophilic bacterium Thermotoga maritima has been purified to homogeneity. A second larger enzyme with PGK activity and identical N-terminal sequence was also found...
- Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeraseB J Eikmanns
Institut für Biotechnologie 1 des Forschungszentrums Jülich GmbH, Germany
J Bacteriol 174:6076-86. 1992..of three glycolytic genes, namely, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene gap, the 3-phosphoglycerate kinase (PGK) gene pgk, and the triosephosphate isomerase (TPI) gene tpi...
- Determination of clonal status of pulmonary sclerosing hemangioma with X-chromosome inactivation mosaicism and polymorphism of phosphoglycerate kinase and androgen receptor genesLi Gong
The Helmholtz Sino German Research Laboratory for Cancer, Department of Pathology, Tangdu Hospital, The Fourth Military Medical University, Xi an, 710038, People s Republic of China
Med Oncol 28:913-8. 2011..of cells using laser microdissection and X-chromosomal inactivation mosaicism and polymorphism at the phosphoglycerate kinase (PGK) and androgen receptor (AR) loci in female somatic cells...
- Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virusJen Wen Lin
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
Nucleic Acids Res 35:424-32. 2007..LC/MS/MS sequencing indicates that p43 is a chloroplast phosphoglycerate kinase (PGK)...
- Substrate-induced double sided H-bond network as a means of domain closure in 3-phosphoglycerate kinaseAndrea Varga
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
FEBS Lett 580:2698-706. 2006Closure of the two domains of 3-phosphoglycerate kinase, upon substrate binding, is essential for the enzyme function...
- Effect of hypoxia on the expression of phosphoglycerate kinase and antitumor activity of troxacitabine and gemcitabine in non-small cell lung carcinomaWing Lam
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510, USA
Mol Cancer Ther 8:415-23. 2009..The cytotoxicity of L-OddC is dependent on its phosphorylation to L-OddCTP by phosphoglycerate kinase (PGK) and its subsequent addition into nuclear DNA...
- Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elementsLuis Quijada
ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
Nucleic Acids Res 30:4414-24. 2002..We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA ..
- Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinaseAtsushi Ikemoto
Mental Health Research Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109 0669, USA
J Biol Chem 278:5929-40. 2003..We demonstrate that the glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate kinase (3-PGK) are enriched in synaptic vesicles, forming a functional complex, and that synaptic vesicles are ..
- Myopathic form of phosphoglycerate kinase (PGK) deficiency: a new case and pathogenic considerationsRonen Spiegel
Department of Pediatrics, Haemek Medical Center, Rappaport School of Medicine, Afula, Israel
Neuromuscul Disord 19:207-11. 2009..b>Phosphoglycerate kinase (PGK) deficiency was documented in muscle and erythrocytes and molecular analysis of the PGK1 gene ..
- Stable transgene expression in human embryonic stem cells after simple chemical transfectionJun Liu
Cell Reprogramming Laboratory, Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Victoria, Australia
Mol Reprod Dev 76:580-6. 2009..We demonstrated that cytomegalovirus (CMV), phosphoglycerate kinase (PGK) and human elongation factor-1alpha (EF1alpha) promoters all resulted in robust activity of a ..
- Hierarchic finite level energy landscape model: to describe the refolding kinetics of phosphoglycerate kinaseSzabolcs Osváth
Department of Biophysics and Radiation Biology, Semmelweis University, Puskin u 9, H 1088 Budapest, Hungary
J Biol Chem 281:24375-80. 2006..Refolding of yeast phosphoglycerate kinase was initiated from the guanidine-unfolded state by stopped-flow or manual mixing and monitored by ..
- Magnetic isotope effect of magnesium in phosphoglycerate kinase phosphorylationAnatoly L Buchachenko
N N Semenov Institute for Chemical Physics, Russian Academy of Sciences, Kosygin Street 4, Moscow 119991, Russia
Proc Natl Acad Sci U S A 102:10793-6. 2005b>Phosphoglycerate kinase (PGK) is found to be controlled by a (25)Mg(2+)-related magnetic isotope effect...
- Inhibiting 3-phosphoglycerate kinase by EDTA stimulates the development of the cleavage stage mouse embryoM Lane
Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
Mol Reprod Dev 60:233-40. 2001..EDTA was shown to inhibit the activity of the glycolytic enzyme, 3-phosphoglycerate kinase. Additionally, the effect of EDTA on maintaining high rates of embryo development in culture could be ..
- Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylasesElise B Daly
Centre for and Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, NSW 2031, Australia
Biochim Biophys Acta 1691:17-22. 2004Solid tumour cells employ glycolytic enzymes including phosphoglycerate kinase (PGK) to make ATP when their supply of oxygen is limiting...
- Molecular characterization of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastorisJoão Ricardo Moreira de Almeida
Laboratorio de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, DF 70910 900, Brazil
Yeast 22:725-37. 2005We report the cloning of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastoris by a PCR approach...
- Role of phosphate chain mobility of MgATP in completing the 3-phosphoglycerate kinase catalytic site: binding, kinetic, and crystallographic studies with ATP and MgATPBeáta Flachner
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, P O Box 7, H 1518 Budapest, Hungary
Biochemistry 43:3436-49. 2004The complexes of pig muscle 3-phosphoglycerate kinase with the substrate MgATP and with the nonsubstrate Mg(2+)-free ATP have been characterized by binding, kinetic, and crystallographic studies...
- Phosphoglycerate kinase 1 a promoting enzyme for peritoneal dissemination in gastric cancerDerek Zieker
Department of General Visceral, Transplant Surgery Comprehensive Cancer Center, University of Tuebingen, D 72076 Tuebingen, Germany
Int J Cancer 126:1513-20. 2010..Previously, we showed elevated mRNA levels of phosphoglycerate kinase 1 (PGK1), an adenosine triphosphate-generating enzyme in the glycolytic pathway, the chemokine receptor 4 ..
- The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coliC E Jones
Department of Biochemistry and Molecular Biology, University College, London, England
Eur J Biochem 233:800-8. 1995The overlapping genes encoding phosphoglycerate kinase (PGK) and glyceraldehyde-3-phosphate dehydrogenase (GraP-DH) from the hyperthermophilic archaeon Sulfolobus solfataricus have been cloned and sequenced...
- Functionally compensating coevolving positions are neither homoplasic nor conserved in cladesGregory B Gloor
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
Mol Biol Evol 27:1181-91. 2010We demonstrated that a pair of positions in phosphoglycerate kinase that score highly by three nonparametric covariation measures are important for function even though the positions can be occupied by aliphatic, aromatic, or charged ..
- Phosphorylation of pyrimidine deoxynucleoside analog diphosphates: selective phosphorylation of L-nucleoside analog diphosphates by 3-phosphoglycerate kinasePreethi Krishnan
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 277:5453-9. 2002..Therefore, roles of creatine kinase, 3-phosphoglycerate kinase, and pyruvate kinase were evaluated using preparations from commercial sources and human HepG2 cells...
- Broad specificity of human phosphoglycerate kinase for antiviral nucleoside analogsSarah Gallois-Montbrun
Unité de Régulation Enzymatique des Activités Cellulaires, CNRS URA 2185, Institut Pasteur, 75724 Paris Cedex 15, France
Biochem Pharmacol 68:1749-56. 2004..Human phosphoglycerate kinase (hPGK) was recently reported to participate in the last step of phosphorylation of cytidine L-nucleotide ..
- Interaction of human 3-phosphoglycerate kinase with L-ADP, the mirror image of D-ADPAndrea Varga
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Karolina Str 29, P O Box 7, H 1518 Budapest, Hungary
Biochem Biophys Res Commun 366:994-1000. 2008..be phosphorylated to pharmacologically active triphosphate forms, the last step seems to involve human 3-phosphoglycerate kinase (hPGK)...
- Substrate-assisted movement of the catalytic Lys 215 during domain closure: site-directed mutagenesis studies of human 3-phosphoglycerate kinaseBeáta Flachner
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
Biochemistry 44:16853-65. 20053-Phosphoglycerate kinase (PGK) is a two-domain hinge-bending enzyme...
- Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformationJun Hyuck Lee
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500 712, Republic of Korea
Biochem Biophys Res Commun 350:1044-9. 2006b>Phosphoglycerate kinase (PGK) is a key glycolytic enzyme that catalyzes the reversible transfer of a phosphate from 1,3-bisphosphoglycerate to ADP to form 3-phosphoglycerate and ATP in the presence of magnesium...
- Role of side-chains in the operation of the main molecular hinge of 3-phosphoglycerate kinaseJudit Szabo
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
FEBS Lett 582:1335-40. 2008..single mutants (F165A, E192A, F196A, S392A, T393A) at and near the main hinge (beta-strand L) of human 3-phosphoglycerate kinase (hPGK) exhibit variously reduced enzyme activity, indicating the cumulative effects of these residues in ..
- Differences in the transient kinetics of the binding of D-ADP and its mirror image L-ADP to human 3-phosphoglycerate kinase revealed by the presence of 3-phosphoglycerateClaire Gondeau
Centre d tudes d agents Pathogènes et Biotechnologies pour la Santé CPBS, UMR 5236, CNRS Université Montpellier 1 Université Montpellier 2, Montpellier Cedex 2, France
Biochemistry 47:3462-73. 2008..The last step of the cascade may be catalyzed by 3-phosphoglycerate kinase (PGK), an enzyme that has low specificity for nucleoside diphosphate (NDP): NDP + 1,3-bisphosphoglycerate ..
- Thermodynamic analysis of the nondenaturational conformational change of baker's yeast phosphoglycerate kinase at 24 degrees COpral Ijeoma
Department of Chemistry and Physics, Texas Woman s University, Bell Avenue, Denton, TX 76204, USA
Arch Biochem Biophys 478:206-11. 2008A plot of the Gibbs free energy of unfolding vs. temperature is calculated for baker's yeast phosphoglycerate kinase in 150 mM sodium phosphate (pH=7...
- A 1.8 A resolution structure of pig muscle 3-phosphoglycerate kinase with bound MgADP and 3-phosphoglycerate in open conformation: new insight into the role of the nucleotide in domain closureA N Szilágyi
Institute of Enzymology Biological Research Center, Hungarian Academy of Sciences, Budapest, H 1518, Hungary
J Mol Biol 306:499-511. 20013-phosphoglycerate kinase (PGK) is a typical kinase with two structural domains. The domains each bind one of the two substrates, 3-phosphoglycerate (3-PG) and MgATP...
- Crystal structures of substrates and products bound to the phosphoglycerate kinase active site reveal the catalytic mechanismB E Bernstein
Department of Biochemistry, University of Washington, Box 357742, Seattle, Washington 98195, USA
Biochemistry 37:4429-36. 1998b>Phosphoglycerate kinase (PGK) catalyzes the reversible phosphoryl transfer between 1,3-bisphosphoglycerate and ADP to form 3-phosphoglycerate and ATP in the presence of magnesium...
- The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complexJ S Anderson
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
EMBO J 17:1497-506. 1998..These observations argue that efficient mRNA turnover is required for viability and that we have identified the two major pathways of mRNA decay in yeast...
- Closed structure of phosphoglycerate kinase from Thermotoga maritima reveals the catalytic mechanism and determinants of thermal stabilityG Auerbach
Max Planck Institut fur Biochemie, Abt Strukturforschung, 82152, Martinsried, Germany
Structure 5:1475-83. 1997b>Phosphoglycerate kinase (PGK) is essential in most living cells both for ATP generation in the glycolytic pathway of aerobes and for fermentation in anaerobes. In addition, in many plants the enzyme is involved in carbon fixation...
- Interaction of human 3-phosphoglycerate kinase with its two substrates: is substrate antagonism a kinetic advantage?Perrine Lallemand
Centre d études d agents Pathogènes et Biotechnologies pour le Santé, UMR 5236, CNRS Universités Montpellier 1 and 2, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
J Mol Biol 409:742-57. 2011..3-Phosphoglycerate kinase (PGK) catalyzes phosphotransfer from 1,3-bisphosphoglycerate (bPG) to ADP to give 3-phosphoglycerate (PG) ..
- Crystallization and preliminary X-ray diffraction analysis of phosphoglycerate kinase from Streptococcus pneumoniaeNoelia Bernardo-García
Department of Crystallography and Structural Biology, Instituto de Quimica Fisica Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1285-9. 2011b>Phosphoglycerate kinase (PGK) is a widespread two-domain enzyme that plays a critical role in the glycolytic pathway...
- Structural and energetic basis of protein kinetic destabilization in human phosphoglycerate kinase 1 deficiencyAngel L Pey
Department of Physical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
Biochemistry 52:1160-70. 2013Protein kinetic destabilization is a common feature of many human genetic diseases. Human phosphoglycerate kinase 1 (PGK1) deficiency is a rare genetic disease caused by mutations in the PGK1 protein, which often shows reduced kinetic ..
- Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plantsShun Fang Cheng
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan
Plant Physiol 163:1598-608. 2013..Previously, we identified that the chloroplast phosphoglycerate kinase (chl-PGK) from Nicotiana benthamiana is one of the viral RNA binding proteins involved in the BaMV ..
- Microarray analysis of rat immune responses to liver fluke infection following vaccination with Fasciola hepatica phosphoglycerate kinaseAgnieszka Wesołowska
Department of Molecular Biology, Laboratory of Molecular Parasitology, W Stefanski Institute of Parasitology, Polish Academy of Sciences, 51 55 Twarda, 00 818 Warsaw, Poland
Exp Parasitol 134:33-8. 2013..hepatica following immunization with F. hepatica phosphoglycerate kinase - a novel vaccine antigen...
- Redox control of the activity of phosphoglycerate kinase in Synechocystis sp. PCC6803Yu Tsukamoto
Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259 R1 8, Midori ku, Yokohama, 226 8503 Japan
Plant Cell Physiol 54:484-91. 2013b>Phosphoglycerate kinase catalyzes the phosphorylation of 3-phosphoglycerate to 1,3-diphosphoglycerate using ATP within the Calvin-Benson cycle. Recent proteomic analysis of thioredoxin targets in Synechocystis sp...
- Molecular insights on pathogenic effects of mutations causing phosphoglycerate kinase deficiencyLaurent R Chiarelli
Dipartimento di Biologia e Biotecnologie L Spallanzani, Universita degli Studi di Pavia, Pavia, Italy
PLoS ONE 7:e32065. 2012b>Phosphoglycerate kinase (PGK) catalyzes an important ATP-generating step in glycolysis...
- Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cellsKasturi Mukherjee
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata 700 032, India
Indian J Biochem Biophys 39:332-41. 20023-Phosphoglycerate kinase (3-PGK) has been purified to apparent homogeneity from Ehrlich ascites carcinoma (EAC) cells by (NH4)2SO4 precipitation, gel filtration and ion-exchange chromatography...
- Dimeric 3-phosphoglycerate kinases from hyperthermophilic Archaea. Cloning, sequencing and expression of the 3-phosphoglycerate kinase gene of Pyrococcus woesei in Escherichia coli and characterization of the protein. Structural and functional comparison D Hess
FB 9 Mikrobiologie, Universitat Essen, Germany
Eur J Biochem 233:227-37. 1995The gene coding for the 3-phosphoglycerate kinase (EC 188.8.131.52) of Pyrococcus woesei was cloned and sequenced. The gene sequence comprises 1230 bp coding for a polypeptide with the theoretical M(r) of 46,195...
- Identification of the actin and plasminogen binding regions of group B streptococcal phosphoglycerate kinaseTyler J Boone
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
J Biol Chem 287:29035-44. 2012b>Phosphoglycerate kinase (PGK), present on the surface of group B streptococcus (GBS), has previously been demonstrated to bind the host proteins actin and plasminogen...
- Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in micePolina V Danshina
Laboratories for Reproductive Biology, Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7090, USA
Biol Reprod 82:136-45. 2010b>Phosphoglycerate kinase 2 (PGK2), an isozyme that catalyzes the first ATP-generating step in the glycolytic pathway, is encoded by an autosomal retrogene that is expressed only during spermatogenesis...
- Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgARoberto Calcedo
Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country, Spain
Rev Iberoam Micol 29:172-4. 2012..Candida albicans is an opportunistic dimorphic fungus commonly present in the human oral cavity that causes infections in immunocompromised patients. The antigen variability, influenced by growth conditions, is a pathogenicity factor...
- Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogasterL Roselli-Rehfuss
Department of Biology, Syracuse University, New York 13244
Mol Gen Genet 235:213-20. 1992The gene that encodes phosphoglycerate kinase in Drosophila melanogaster (Pgk) has been isolated and characterized. There is a single copy of Pgk in the Drosophila genome located at cytogenetic position 23A1-2. Transcripts of Pgk are 1...
- An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomeraseP Branny
Laboratoire d Enzymologie et de Biochimie Structurales du CNRS, Gif sur Yvette, France
Microbiology 144:905-14. 1998..gap, pgk and tpi encoding three glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI), respectively, have been cloned and sequenced from ..
- Comparing the folding and misfolding energy landscapes of phosphoglycerate kinaseGergely Agócs
Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
Biophys J 102:2828-34. 2012..Using yeast phosphoglycerate kinase as model, here we identify the features of the energy landscape that decide the fate of the protein: ..
- A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cellsMingang Xu
Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, Philadelphia, PA 6080, USA
Nucleic Acids Res 36:7157-67. 2008b>Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis...
- Functional identity of a primer recognition protein as phosphoglycerate kinaseH K Jindal
Department of Biochemistry, University of Nebraska Medical Center, Omaha 68105
J Biol Chem 265:6540-3. 1990..search against a protein sequence data base, we have identified PRP 2 to be the glycolytic enzyme, phosphoglycerate kinase (PGK). The activities of PRP and PGK increase coordinately in the PRP purification procedure...
- PGK1, the gene encoding the glycolitic enzyme phosphoglycerate kinase, acts as a multicopy suppressor of apoptotic phenotypes in S. cerevisiaeCristina Mazzoni
Pasteur Institute Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
Yeast 26:31-7. 2009..by overexpressing yeast genes allowed the isolation of PGK1, the gene encoding the glycolytic enzyme phosphoglycerate kinase. This gene restored normal ageing, oxygen peroxide resistance and nuclear integrity in the mutant...
- Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complexE M Eicher
Mol Gen Genet 158:225-8. 1978The mouse autosomal locus that determines the form of phosphoglycerate kinase found only in testes is shown here to be closely linked to but not included within the major histocompatibility complex on chromosome 17...
- Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycinC M Grant
Biological Laboratory, University of Kent, Canterbury, UK
Curr Genet 26:95-9. 1994..However, paromomycin-induced mistranslation of a heterologous mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen...
- Molecular defect of a phosphoglycerate kinase variant (PGK-Matsue) associated with hemolytic anemia: Leu----Pro substitution caused by T/A----C/G transition in exon 3M Maeda
Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, CA 91010
Blood 77:1348-52. 1991We have identified the mutation in a phosphoglycerate kinase variant (PGK-Matsue) associated with severe enzyme deficiency, congenital nonspherocytic hemolytic anemia, and mental disorders...
- Molecular abnormalities of a phosphoglycerate kinase variant generated by spontaneous mutationM Maeda
Department of Biochemical Genetics, Beckman Research Institute, City of Hope, Duarte, CA 91010
Blood 79:2759-62. 1992A new case of X chromosome-linked phosphoglycerate kinase (PGK) abnormality is described. The male proband was mentally retarded, had behavior disorders, and displayed episodes of hemolytic anemia...
- Enzymatic activity of immobilized yeast phosphoglycerate kinaseCedric Hurth
Centre de Physique Moleculaire Optique et Hertzienne, 33600 Pessac, France
Biosens Bioelectron 22:2449-55. 2007This work reports the first evidence that recombinant yeast phosphoglycerate kinase (PGK) is still significantly active when immobilized on glass and muscovite mica...
- The identification of a recurrent phosphoglycerate kinase mutation associated with chronic haemolytic anaemia and neurological dysfunction in a family from USAJonathan M Flanagan
Department of Molecular and Experimental Medicine, The Scripps Research Institute, MEM 215, 10550 Torrey Pines, La Jolla, CA, USA
Br J Haematol 134:233-7. 2006b>Phosphoglycerate kinase (PGK) deficiency is a rare X-linked disease that is characterised by mild to severe haemolytic anaemia, rhabdomyolysis, and variable defects in the central nervous system...
- Altered expression of PGK1 in a family with phosphoglycerate kinase deficiencyEva K Svaasand
Department of Pathology and Medical Genetics, St Olav Hospital, Trondheim, Norway
Muscle Nerve 36:679-84. 2007The X-linked recessive disease phosphoglycerate kinase (PGK) deficiency is caused by altered expression of the PGK1 enzyme, which causes muscle stiffness, hemolytic anemia, and mental retardation...
- Gene expression and clonality analysis of the androgen receptor and phosphoglycerate kinase genes in polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangiomaEn Hua Wang
Department of Pathology, College of Basic Medical Sciences, China Medical University, and First Affiliated Hospital of China Medical University, Shenyang, China
Mod Pathol 20:1208-15. 2007..with the methylation-sensitive enzymes HhaI or HpaII, followed by nested PCR of the androgen receptor and phosphoglycerate kinase genes. Samples with polymorphisms were identified and a clonality analysis was performed...
- A single amino acid substitution (157 Gly----Val) in a phosphoglycerate kinase variant (PGK Shizuoka) associated with chronic hemolysis and myoglobinuriaH Fujii
Department of Blood Transfusion Medicine, Tokyo Women s Medical College, Japan
Blood 79:1582-5. 1992We have determined a single amino acid substitution in a new phosphoglycerate kinase (PGK) variant, PGK Shizuoka, associated with chronic hemolysis and myoglobinuria...
- Phosphoglycerate kinase deficiency in two brothers with McArdle-like clinical symptomsJ Aasly
Department of Neurology, University Hospital, Trondheim, Norway
Eur J Neurol 7:111-3. 2000b>Phosphoglycerate kinase (PGK) catalyses the transfer of the acylphosphate group of 1,3-diphosphoglycerate to ADP with formation of 3-phosphoglycerate and ATP in the terminal stage of the glycolytic pathway...
- Intronic mutation in the PGK1 gene may cause recurrent myoglobinuria by aberrant splicingKentaro Shirakawa
First Department of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan
Neurology 66:925-7. 2006A 33-year-old man with mental retardation and recurrent myoglobinuria demonstrated a deficiency in the phosphoglycerate kinase 1 (PGK1) activity of his muscles and erythrocytes...
- Insight into the mechanism of domain movements and their role in enzyme function: example of 3-phosphoglycerate kinaseM Vas
Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
Curr Protein Pept Sci 11:118-47. 2010..In this review we summarize comprehensive studies with a typical hinge-bending two-domain enzyme, 3-phosphoglycerate kinase. A possible mechanism is proposed by which the two substrates that bind to different domains trigger the ..
- Identification of new mutations in two phosphoglycerate kinase (PGK) variants expressing different clinical syndromes: PGK Créteil and PGK AmiensM Cohen-Solal
Unité INSERM U91, Hopital Henri Mondor, Creteil, France
Blood 84:898-903. 1994b>Phosphoglycerate kinase (PGK) deficiency is generally associated with chronic hemolytic anemia, although it can be accompanied by either mental retardation or muscular disease...
- Retarded and aberrant splicings caused by single exon mutation in a phosphoglycerate kinase variantT Ookawara
Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
Arch Biochem Biophys 327:35-40. 1996The molecular abnormality of a phosphoglycerate kinase variant which was associated with severe tissue enzyme deficiency and episodes of muscle contractions and myoglobinuria was examined...
- Molecular abnormality of a phosphoglycerate kinase variant (PGK-Alabama)A Yoshida
Department of Biochemical Genetics, Beckman Institute of the City of Hope, Duarte, California, USA
Blood Cells Mol Dis 21:179-81. 1995The molecular abnormality of a phosphoglycerate kinase variant associated with severe red cell enzyme deficiency ( about 4% of normal) and episodes of hemolysis with jaundice was examined...
- Two proliferation-related proteins, TYMS and PGK1, could be new cytotoxic T lymphocyte-directed tumor-associated antigens of HLA-A2+ colon cancerShigeki Shichijo
Department of Immunology, Kurume University School of Medicine, Research Center of Innovative Cancer Therapy of the 21st Century COE Program for Medical Science, Kurume University, Kurume, Fukuoka, Japan
Clin Cancer Res 10:5828-36. 2004..The purpose of this work was to provide a scientific basis for specific immunotherapy of colon cancer...
- Substrate binding modifies the hinge bending characteristics of human 3-phosphoglycerate kinase: a molecular dynamics studyZoltan Palmai
Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Hungary
Proteins 77:319-29. 2009..At the same time, ligand binding results in an enhanced correlation of internal domain motions...
- A single amino acid substitution (Asp leads to Asn) in a phosphoglycerate kinase variant (PGK München) associated with enzyme deficiencyH Fujii
J Biol Chem 255:6421-3. 1980The structural abnormality of the phosphoglycerate kinase variant, PGK München, associated with red cell enzyme deficiency and heat instability, was elucidated by a microscale peptide-mapping method...
- Enzymatic and metabolic characterization of the phosphoglycerate kinase deficiency associated with chronic hemolytic anemia caused by the PGK-Barcelona mutationMaría José Ramírez-Bajo
Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Spain
Blood Cells Mol Dis 46:206-11. 2011Recently, we reported a new mutation of phosphoglycerate kinase (PGK), called PGK-Barcelona, which causes chronic hemolytic anemia associated with progressive neurological impairment...
- A phosphoglycerate kinase mutant (PGK Herlev; D285V) in a Danish patient with isolated chronic hemolytic anemia: mechanism of mutation and structure-function relationshipsC Valentin
Unité INSERM U 474, Hopital Henri Mondor, Creteil, France
Hum Mutat 12:280-7. 1998b>Phosphoglycerate kinase (PGK) is a X-linked enzyme that plays a key role in the glycolytic pathway. Twelve different variants have already been reported...
- Molecular abnormality of phosphoglycerate kinase-Uppsala associated with chronic nonspherocytic hemolytic anemiaH Fujii
Proc Natl Acad Sci U S A 77:5461-5. 1980Inherited deficiency of phosphoglycerate kinase (PGK; ATP:3-phosphoglycerate 1-phosphotransferase, EC 184.108.40.206) is associated with chronic nonspherocytic hemolytic anemia and mental disorders in man...
- Use of cultured lymphoblastoid cells for the study of abnormal enzymes: molecular abnormality of a phosphoglycerate kinase variant associated with hemolytic anemiaH Fujii
Proc Natl Acad Sci U S A 78:2587-90. 1981A phosphoglycerate kinase (PGKase: ATP:3-phosphoglycerate 1-phosphotransferase, EC 220.127.116.11; X chromosome-linked) variant, PGKase-Tokyo, is associated with enzyme deficiency, nonspherocytic hemolytic anemia, and neurological disturbances...
- Phosphoglycerate kinase 1-overexpressing lung cancer cells reduce cyclooxygenase 2 expression and promote anti-tumor immunity in vivoShye Jye Tang
Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, ROC
Int J Cancer 123:2840-8. 2008In addition to the known function in the glycolytic pathway, phosphoglycerate kinase 1 (PGK-1) promotes reduction of plasmin disulfide bonds leading to angiostatin formation and inhibition of tumor angiogenesis...
- Variation for X chromosome expression in mice detected by electrophoresis of phosphoglycerate kinaseJ D West
Genet Res 32:91-102. 1978
- Native and unfolded states of phosphoglycerate kinase studied by single-molecule FRETTobias Rosenkranz
Research Centre Julich, ISB 2 Molecular Biophysics, 52425 Julich, Germany
Chemphyschem 12:704-10. 2011Single-molecule Förster resonance energy transfer (FRET) measurements with phosphoglycerate kinase from yeast were performed at different concentrations of guanidine hydrochloride...
- A novel missense mutation (1060G --> C) in the phosphoglycerate kinase gene in a Japanese boy with chronic haemolytic anaemia, developmental delay and rhabdomyolysisAkira Morimoto
Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan
Br J Haematol 122:1009-13. 2003We report the case of a 3-year-old Japanese boy with phosphoglycerate kinase 1 (PGK1) deficiency (Online Mendelian Inheritance in Man entry 311800)...
- A sandwich ELISA for phosphoglycerate kinaseWeilin Zhao
Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
J Immunoassay Immunochem 29:220-33. 2008b>Phosphoglycerate kinase (PGK1) is a key enzyme in glycolysis that can also be released from certain cells...
- Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expressionMing Yi Ho
Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Department of Education and Research, Taipei City Hospital, Taipei, Taiwan
Cancer Sci 101:2411-6. 2010b>Phosphoglycerate kinase 1 (PGK-1) is a multifunctional protein that is involved in the glycolytic pathway and the generation of the angiogenesis inhibitor angiostatin...
- Blocking Fasciola hepatica's energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinaseSlawomir Jaros
Department of Molecular Biology, Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland
Vet Parasitol 172:229-37. 2010..This paper is focused on first vaccination trials based on a new vaccine candidate antigen, F. hepatica phosphoglycerate kinase (FhPGK) performed on a rat model...
- Immunoelectron microscopic analysis of the intracellular distribution of primer recognition proteins, annexin 2 and phosphoglycerate kinase, in normal and transformed cellsK D Kumble
Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
J Cell Sci 99:751-8. 1991..We have previously reported that the PRP consist of a complex of two proteins identified as 3-phosphoglycerate kinase (PGK) and the protein-tyrosine kinase substrate, annexin 2 monomer...
- Denatured states of yeast phosphoglycerate kinaseG Damaschun
Institut für Biologie der Humboldt Universität zu Berlin, Germany
Biochemistry (Mosc) 63:259-75. 1998..Acid-denatured, cold-denatured, and 6 M guanidine hydrochloride (GuHCl) denatured yeast phosphoglycerate kinase (PGK) are ensembles of flexible unfolded molecules with rapidly interconverting structures of the ..
- Processing of a phosphoglycerate kinase reporter mRNA in Trypanosoma brucei is not coupled to transcription by RNA polymerase IIMhairi Stewart
Zentrum für Molekularbiologie der Universität Heidelberg, ZMBH DKFZ Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
Mol Biochem Parasitol 172:99-106. 2010..There was therefore no evidence for recruitment of the 3'-splicing apparatus by the RNA polymerase. Abundant transcripts transcribed from the rRNA locus by T7 RNA polymerase were somewhat less efficiently processed...
- Structural tightening and interdomain communication in the catalytic cycle of phosphoglycerate kinaseJames P Marston
Department of Molecular Biology and Biotechnology, Firth Court, The University of Sheffield, Western Bank, Sheffield, UK
J Mol Biol 396:345-60. 2010Changes in amide-NH chemical shift and hydrogen exchange rates as phosphoglycerate kinase progresses through its catalytic cycle have been measured to assess whether they correlate with changes in hydrogen bonding within the protein...
- Entry of Spiroplasma citri into Circulifer haematoceps cells involves interaction between spiroplasma phosphoglycerate kinase and leafhopper actinFabien Labroussaa
UMR 1090 Génomique Diversité Pouvoir Pathogène, INRA, Universite Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP 81, F 33883 Villenave d Ornon, France
Appl Environ Microbiol 76:1879-86. 2010..actin-binding protein of 44 kDa was isolated by affinity chromatography and identified by LC-MS/MS as phosphoglycerate kinase (PGK)...
- The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomesAntonio M Estevez
Instituto de Parasitologia y Biomedicina Lopez Neyra, CSIC Avda del Conocimiento s n, Armilla, 18100 Granada, Spain
Nucleic Acids Res 36:4573-86. 2008..These observations raise the possibility that TbDRBD3-mRNA complexes act as a post-transcriptional operon, and provide a framework to interpret how trypanosomes regulate gene expression in the absence of transcriptional control...
- Foamy-virus-mediated gene transfer to canine repopulating cellsHans Peter Kiem
Clinical Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N D1 100, PO Box 19024, Seattle, WA 98109 1024, USA
Blood 109:65-70. 2007..These data suggest that FV vectors should be useful for stem-cell gene therapy, particularly for applications in which short transduction protocols are critical...
- Glycolysis and Glutamate Storage into Synaptic VesiclesTetsufumi Ueda; Fiscal Year: 2010..We have recently provided evidence that glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase, glycolytic enzymes responsible for production of ATP, are associated with synaptic vesicles and that ..
- Inhibitors of Trypanosomatid Phosphoglycerate Mutase and Phosphoglycerate KinaseMALCOLM DOUGLAS WALKINSHAW; Fiscal Year: 2012..of this project is to discover and optimize selective inhibitors of phosphoglycerate mutase (PGAM) and phosphoglycerate kinase (PGK) of trypanosomatid parasites (Trypanosoma brucei, T. cruzi and Leishmania species)...
- Lentiviral Vectors for the Treatment of Fanconi AnemiaDAVID W CLAPP; Fiscal Year: 2010..1: Generate recombinant lentiviral constructs that encode the expression of the FANCA cDNA by the human phosphoglycerate kinase promoter or the elongation factor 1a promoter and test functional expression of the transgenes in murine ..
- NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDSYung Chi Cheng; Fiscal Year: 2010..2. To examine the role of Hypoxia: a. in regulating phosphoglycerate kinase (PGK), which is responsible for the phosphorylation of L- OddCDP to L-OddCTP. b...
- CONTROL OF TRANSCRIPTION OF ISOLATED EUCARYOTIC GENESMichael Holland; Fiscal Year: 1980..DNAs containing genomic DNA sequences which code for yeast glyceraldehyde 3-phosphate dehydrogenase, phosphoglycerate kinase, enolase and phosphoglycerate mutase have been isolated in this laboratory and will be fundamental to the ..
- X-CHROMOSOME INACTIVATION IN OPOSSUM DEVELOPMENTPAUL SAMOLLOW; Fiscal Year: 1991..relative levels of expression of the Gpd and Pgk-A genes (encode glucose-6-phosphate dehydrogenase and phosphoglycerate kinase-A, respectively on the maternal and paternal X chromosomes in tissues and organs of newborn pouch young, ..
- MOLECULAR PATHOPHYSIOLOGY OF THYROID CELL GROWTHJames Fagin; Fiscal Year: 1993..advantage of polymorphisms in the X-chromosome genes hypoxanthine phosphorybosiltransferase (HPRT) and phosphoglycerate kinase (PGK)...
- CLONALITY IN ACUTE MYELOID LEUKEMIA AND MYELODYSPLASIADonald Kufe; Fiscal Year: 1992..via noting allelic loss of a restriction fragment length polymorphism (RFLP) associated with the X-linked phosphoglycerate kinase (PGK) gene...
- GENE REGULATION IN SPERMATOGENESISJohn McCarrey; Fiscal Year: 1992The mammalian phosphoglycerate kinase (PGK) gene family remains unparalleled in the number of different facets of gene regulation that impinge upon the expression of these genes in spermatogenesis...
- TIME-RESOLVED FLUORESCENCE STUDIES OF PROTEIN FOLDINGJoseph Beechem; Fiscal Year: 1999..Experimental efforts are concentrated on the examination of the folding of yeast phosphoglycerate kinase (PGK) and E. coli alpha- subunit of tryptophan synthase (alpha-TS...
- MEMBRANE TRANSPORT PROCESSES IN RED BLOOD CELLSJoseph Hoffman; Fiscal Year: 1991..situations, these parameters will also be followed together with the possible involvement of membrane phosphoglycerate kinase and Na,K-ATPase defining a membrane pool of ATP...
- ADAPTATION TO ENVIRONMENTAL STRESS--MECHANISTIC ANALYSESJames Whitlock; Fiscal Year: 2000..The experiments involve analyses of the response of the phosphoglycerate kinase 1 and aldolase A genes to hypoxia, genetic analyses of wild-type and mutant forms of HIF1a, and ..
- DOMAIN INTERACTION IN GLOBULAR AND TRANSMEMBRANE PROTEINJOHN BRANDTS; Fiscal Year: 1993..A well-defined, two-domain, soluble enzyme from yeast- 3-phosphoglycerate kinase (PG32, and 2.) A membrane- spanning protein, the aspartate receptor (AR) of S...
- NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDSYung Chi Cheng; Fiscal Year: 2007..2. To examine the role of Hypoxia: a. in regulating phosphoglycerate kinase (PGK), which is responsible for the phosphorylation of L- OddCDP to L-OddCTP. b...
- AGE RELATED CONFORMATIONAL MODIFICATIONS OF PROTEINAri Gafni; Fiscal Year: 2003..We will focus on two proteins, the glycolytic enzyme phosphoglycerate kinase (PGK) and the fibrinolysis-associated protein plasminogen activator inhibitor-1 (PAI-1)...
- Regulation of Gene Expression by DNA MethylationJohn McCarrey; Fiscal Year: 2002..This is the case for the autosomal phosphoglycerate kinase (Pgk2), which is expressed specifically in spermatogenic cells...
- SUBMILLISECOND LASER T JUMP INDUCED PROTEIN FOLDINGMartin Gruebele; Fiscal Year: 2001..Our initially proposed systems include apomyoglobin, cytochrome c, ubiquitin, and phosphoglycerate kinase, all of which have known sub-ms folding phases...
- IMMUNOGENETIC STUDIES OF A SPERM ENZYME PGK-BJOHN VANDEBERG; Fiscal Year: 1980The overall objectives are to characterize the sperm enzyme phosphoglycerate kinase B (PGK-B) immunogenetically and immunochemically and to determine its role in reproduction...
- CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASESreerama Shetty; Fiscal Year: 2004..Our preliminary data support the hypothesis and show that phosphoglycerate kinase (PGK) and other newly recognized uPAR and uPA mRNA binding protein-mRNA interactions control uPAR and uPA ..
- Regulation of Lung Epithelial Fibrinolysis by UrokinaseSreerama Shetty; Fiscal Year: 2005..This information could hasten the development of new, mechanism-based interventions for lung disorders, including acute lung injury or lung neoplasia. ..
- Single Cell Analysis of Cross Talk Among Kinase PathwaysStefan Kaluz; Fiscal Year: 2008..These studies will provide additional information relating to our understanding of the consequencesof activationof kinase signaling pathwayswith respect to malignant transformation, aggressive tumor growth and survival. ..
- Cord Blood Expansion and Transplantation in a Nonhuman Primate ModelHans Peter Kiem; Fiscal Year: 2009....
- Gene therapy for Fanconi anemiaHans Peter Kiem; Fiscal Year: 2009..Our ultimate goal is to improve or cure the hematopoietic symptoms in FA patients by lentivirus-mediated HSC gene transfer, and develop methodologies that would be applicable to other genetic disorders and hematological diseases. ..
- IL-12-Faciliated Hematopoietic Recovery Following Myeloablative TherapyDan Douer; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- CLINICAL RESEARCH CENTER FOR NEUROMUSCULAR DISEASESalvatore DiMauro; Fiscal Year: 2007....
- MGMT-Mediated in vivo Selection in a Large Animal ModelHans Peter Kiem; Fiscal Year: 2006..These experiments will provide a platform for studies using gene-modified allogeneic stem cells to improve nonmyeloablative transplant strategies. ..
- Testicular Torsion: Mechanisms and TreatmentTerry Turner; Fiscal Year: 2005..It is likely that these investigations will be relevant to other forms of oxidative stress inducing testicular dysfunction. ..
- Arsenic Trioxide and Vitamin C in AMLDan Douer; Fiscal Year: 2005..In summary, this combination may have higher benefit/risk ratio in non-APL AML patients resulting in an overall better outcome with less toxicity than conventional chemotherapy. ..
- A Mouse Model of Gaucher DiseaseErnest Beutler; Fiscal Year: 2005..In particular, electron microscopy and light microscopy will be used to attempt to demonstrate the development of Gaucher cells in the chimeric mice. ..
- Transcriptional Regulation of a Surfactant EnzymeRAMA MALLAMPALLI; Fiscal Year: 2003..Ultimately, the results from these studies could lead to therapeutic strategies directed at modulating expression of this key surfactant enzyme at the molecular level. ..
- RETROVIRAL TRANSFER OF ANKYRIN FOR SPHEROCYTOSISPamela Becker; Fiscal Year: 2002..abstract_text> ..
- FORMATION AND SURVIVAL OF RED BLOOD CELLSErnest Beutler; Fiscal Year: 2001..Should these point mutations fail to alter gene transcription, a search will be made for other potential mutations in linkage disequilibrium with the known -5,-8 and -24 polymorphisms, by sequencing upstream through the promotor. ..
- Hematopoietic Potential of Muscle-Derived CellsHans Peter Kiem; Fiscal Year: 2004..abstract_text> ..