phosphoglycerate kinase

Summary

Summary: An enzyme catalyzing the transfer of a phosphate group from 3-phospho-D-glycerate in the presence of ATP to yield 3-phospho-D-glyceroyl phosphate and ADP. EC 2.7.2.3.

Top Publications

  1. pmc The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice
    Cyntia G M C Trant
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil
    Infect Immun 78:2283-91. 2010
  2. pmc Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus
    Jen Wen Lin
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
    Nucleic Acids Res 35:424-32. 2007
  3. pmc A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase
    Louiza Zerrad
    Structural Biology Group, European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F 38043 Grenoble, France
    J Biol Chem 286:14040-8. 2011
  4. pmc Molecular basis for the lack of enantioselectivity of human 3-phosphoglycerate kinase
    C Gondeau
    Centre d Etudes d Agents Pathogenes et Biotechnologies pour la Sante, UMR 5236, CNRS Universités Montpellier 1 et 2, Institut de Biologie, 4 Bd Henri IV, CS69033, 34965 Montpellier cedex 2, France
    Nucleic Acids Res 36:3620-9. 2008
  5. doi Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis
    Matthew J Cliff
    The Krebs Institute and The Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Am Chem Soc 132:6507-16. 2010
  6. doi Interaction of human 3-phosphoglycerate kinase with its two substrates: is substrate antagonism a kinetic advantage?
    Perrine Lallemand
    Centre d études d agents Pathogènes et Biotechnologies pour le Santé, UMR 5236, CNRS Universités Montpellier 1 and 2, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Mol Biol 409:742-57. 2011
  7. ncbi PGK deficiency
    Ernest Beutler
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Br J Haematol 136:3-11. 2007
  8. ncbi Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase
    Atsushi Ikemoto
    Mental Health Research Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109 0669, USA
    J Biol Chem 278:5929-40. 2003
  9. doi Stable transgene expression in human embryonic stem cells after simple chemical transfection
    Jun Liu
    Cell Reprogramming Laboratory, Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Victoria, Australia
    Mol Reprod Dev 76:580-6. 2009
  10. doi Myopathic form of phosphoglycerate kinase (PGK) deficiency: a new case and pathogenic considerations
    Ronen Spiegel
    Department of Pediatrics, Haemek Medical Center, Rappaport School of Medicine, Afula, Israel
    Neuromuscul Disord 19:207-11. 2009

Research Grants

Detail Information

Publications266 found, 100 shown here

  1. pmc The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice
    Cyntia G M C Trant
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil
    Infect Immun 78:2283-91. 2010
    ..Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308...
  2. pmc Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus
    Jen Wen Lin
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
    Nucleic Acids Res 35:424-32. 2007
    ..LC/MS/MS sequencing indicates that p43 is a chloroplast phosphoglycerate kinase (PGK)...
  3. pmc A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase
    Louiza Zerrad
    Structural Biology Group, European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F 38043 Grenoble, France
    J Biol Chem 286:14040-8. 2011
    b>Phosphoglycerate kinase (PGK) is the enzyme responsible for the first ATP-generating step of glycolysis and has been implicated extensively in oncogenesis and its development...
  4. pmc Molecular basis for the lack of enantioselectivity of human 3-phosphoglycerate kinase
    C Gondeau
    Centre d Etudes d Agents Pathogenes et Biotechnologies pour la Sante, UMR 5236, CNRS Universités Montpellier 1 et 2, Institut de Biologie, 4 Bd Henri IV, CS69033, 34965 Montpellier cedex 2, France
    Nucleic Acids Res 36:3620-9. 2008
    ..to reveal the molecular basis for the low enantioselectivity and the broad specificity of human 3-phosphoglycerate kinase (hPGK), an enzyme responsible for the last step of phosphorylation of many nucleotide derivatives...
  5. doi Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis
    Matthew J Cliff
    The Krebs Institute and The Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Am Chem Soc 132:6507-16. 2010
    Transition state analogue (TSA) complexes formed by phosphoglycerate kinase (PGK) have been used to test the hypothesis that balancing of charge within the transition state dominates enzyme-catalyzed phosphoryl transfer...
  6. doi Interaction of human 3-phosphoglycerate kinase with its two substrates: is substrate antagonism a kinetic advantage?
    Perrine Lallemand
    Centre d études d agents Pathogènes et Biotechnologies pour le Santé, UMR 5236, CNRS Universités Montpellier 1 and 2, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Mol Biol 409:742-57. 2011
    ..3-Phosphoglycerate kinase (PGK) catalyzes phosphotransfer from 1,3-bisphosphoglycerate (bPG) to ADP to give 3-phosphoglycerate (PG) ..
  7. ncbi PGK deficiency
    Ernest Beutler
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Br J Haematol 136:3-11. 2007
    b>Phosphoglycerate kinase (PGK) deficiency is one of the relatively uncommon causes of hereditary non-spherocytic haemolytic anaemia (HNSHA). The gene encoding the erythrocyte enzyme PGK1, is X-linked...
  8. ncbi Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase
    Atsushi Ikemoto
    Mental Health Research Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109 0669, USA
    J Biol Chem 278:5929-40. 2003
    ..We demonstrate that the glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate kinase (3-PGK) are enriched in synaptic vesicles, forming a functional complex, and that synaptic vesicles are ..
  9. doi Stable transgene expression in human embryonic stem cells after simple chemical transfection
    Jun Liu
    Cell Reprogramming Laboratory, Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Victoria, Australia
    Mol Reprod Dev 76:580-6. 2009
    ..We demonstrated that cytomegalovirus (CMV), phosphoglycerate kinase (PGK) and human elongation factor-1alpha (EF1alpha) promoters all resulted in robust activity of a ..
  10. doi Myopathic form of phosphoglycerate kinase (PGK) deficiency: a new case and pathogenic considerations
    Ronen Spiegel
    Department of Pediatrics, Haemek Medical Center, Rappaport School of Medicine, Afula, Israel
    Neuromuscul Disord 19:207-11. 2009
    ..b>Phosphoglycerate kinase (PGK) deficiency was documented in muscle and erythrocytes and molecular analysis of the PGK1 gene ..
  11. ncbi Substrate-induced double sided H-bond network as a means of domain closure in 3-phosphoglycerate kinase
    Andrea Varga
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    FEBS Lett 580:2698-706. 2006
    Closure of the two domains of 3-phosphoglycerate kinase, upon substrate binding, is essential for the enzyme function...
  12. doi PGK1, the gene encoding the glycolitic enzyme phosphoglycerate kinase, acts as a multicopy suppressor of apoptotic phenotypes in S. cerevisiae
    Cristina Mazzoni
    Pasteur Institute Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Yeast 26:31-7. 2009
    ..by overexpressing yeast genes allowed the isolation of PGK1, the gene encoding the glycolytic enzyme phosphoglycerate kinase. This gene restored normal ageing, oxygen peroxide resistance and nuclear integrity in the mutant...
  13. ncbi Functional identity of a primer recognition protein as phosphoglycerate kinase
    H K Jindal
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68105
    J Biol Chem 265:6540-3. 1990
    ..search against a protein sequence data base, we have identified PRP 2 to be the glycolytic enzyme, phosphoglycerate kinase (PGK). The activities of PRP and PGK increase coordinately in the PRP purification procedure...
  14. pmc Comparing the folding and misfolding energy landscapes of phosphoglycerate kinase
    Gergely Agócs
    Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
    Biophys J 102:2828-34. 2012
    ..Using yeast phosphoglycerate kinase as model, here we identify the features of the energy landscape that decide the fate of the protein: ..
  15. pmc Phosphoglycerate kinase and triosephosphate isomerase from the hyperthermophilic bacterium Thermotoga maritima form a covalent bifunctional enzyme complex
    H Schurig
    Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, Germany
    EMBO J 14:442-51. 1995
    b>Phosphoglycerate kinase (PGK) from the hyperthermophilic bacterium Thermotoga maritima has been purified to homogeneity. A second larger enzyme with PGK activity and identical N-terminal sequence was also found...
  16. pmc Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression
    Jianhua Wang
    Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China
    Cancer Res 70:471-80. 2010
    ..microdissection and cDNA microarray analysis of CAFs in prostate tumors revealed strong upregulation of phosphoglycerate kinase-1 (PGK1), an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway and is directly ..
  17. doi Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinase
    Judit Szabo
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, PO Box 7, Hungary, Germany
    Biochemistry 47:6735-44. 2008
    3-Phosphoglycerate kinase is a hinge-bending enzyme with substrate-assisted domain closure. However, the closure mechanism has not been described in terms of structural details...
  18. doi Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgA
    Roberto Calcedo
    Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country, Spain
    Rev Iberoam Micol 29:172-4. 2012
    ..Candida albicans is an opportunistic dimorphic fungus commonly present in the human oral cavity that causes infections in immunocompromised patients. The antigen variability, influenced by growth conditions, is a pathogenicity factor...
  19. pmc Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice
    Polina V Danshina
    Laboratories for Reproductive Biology, Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7090, USA
    Biol Reprod 82:136-45. 2010
    b>Phosphoglycerate kinase 2 (PGK2), an isozyme that catalyzes the first ATP-generating step in the glycolytic pathway, is encoded by an autosomal retrogene that is expressed only during spermatogenesis...
  20. ncbi Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex
    E M Eicher
    Mol Gen Genet 158:225-8. 1978
    The mouse autosomal locus that determines the form of phosphoglycerate kinase found only in testes is shown here to be closely linked to but not included within the major histocompatibility complex on chromosome 17...
  21. ncbi An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase
    P Branny
    Laboratoire d Enzymologie et de Biochimie Structurales du CNRS, Gif sur Yvette, France
    Microbiology 144:905-14. 1998
    ..gap, pgk and tpi encoding three glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI), respectively, have been cloned and sequenced from ..
  22. doi Crystal structure of Plasmodium falciparum phosphoglycerate kinase: evidence for anion binding in the basic patch
    Craig D Smith
    University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochem Biophys Res Commun 412:203-6. 2011
    3-Phosphoglycerate kinase (EC 2.7.2.3) is a key enzyme in the glycolytic pathway and catalyzes an important phosphorylation step leading to the production of ATP...
  23. ncbi Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster
    L Roselli-Rehfuss
    Department of Biology, Syracuse University, New York 13244
    Mol Gen Genet 235:213-20. 1992
    The gene that encodes phosphoglycerate kinase in Drosophila melanogaster (Pgk) has been isolated and characterized. There is a single copy of Pgk in the Drosophila genome located at cytogenetic position 23A1-2. Transcripts of Pgk are 1...
  24. pmc A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells
    Mingang Xu
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, Philadelphia, PA 6080, USA
    Nucleic Acids Res 36:7157-67. 2008
    b>Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis...
  25. doi Binding of group B streptococcal phosphoglycerate kinase to plasminogen and actin
    Tyler J Boone
    The Department of Laboratory Medicine and Pathology, The University of Alberta, Edmonton, Alberta, Canada
    Microb Pathog 51:255-61. 2011
    The glycolytic enzyme, phosphoglycerate kinase (PGK) of group B streptococci (GBS), has previously been identified as expressed on the GBS cell surface...
  26. ncbi Phosphoglycerate kinase deficiency in two brothers with McArdle-like clinical symptoms
    J Aasly
    Department of Neurology, University Hospital, Trondheim, Norway
    Eur J Neurol 7:111-3. 2000
    b>Phosphoglycerate kinase (PGK) catalyses the transfer of the acylphosphate group of 1,3-diphosphoglycerate to ADP with formation of 3-phosphoglycerate and ATP in the terminal stage of the glycolytic pathway...
  27. doi Phosphoglycerate kinase 1-overexpressing lung cancer cells reduce cyclooxygenase 2 expression and promote anti-tumor immunity in vivo
    Shye Jye Tang
    Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, ROC
    Int J Cancer 123:2840-8. 2008
    In addition to the known function in the glycolytic pathway, phosphoglycerate kinase 1 (PGK-1) promotes reduction of plasmin disulfide bonds leading to angiostatin formation and inhibition of tumor angiogenesis...
  28. ncbi Insight into the mechanism of domain movements and their role in enzyme function: example of 3-phosphoglycerate kinase
    M Vas
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    Curr Protein Pept Sci 11:118-47. 2010
    ..In this review we summarize comprehensive studies with a typical hinge-bending two-domain enzyme, 3-phosphoglycerate kinase. A possible mechanism is proposed by which the two substrates that bind to different domains trigger the ..
  29. ncbi Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycin
    C M Grant
    Biological Laboratory, University of Kent, Canterbury, UK
    Curr Genet 26:95-9. 1994
    ..However, paromomycin-induced mistranslation of a heterologous mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen...
  30. ncbi A phosphoglycerate kinase mutant (PGK Herlev; D285V) in a Danish patient with isolated chronic hemolytic anemia: mechanism of mutation and structure-function relationships
    C Valentin
    Unité INSERM U 474, Hopital Henri Mondor, Creteil, France
    Hum Mutat 12:280-7. 1998
    b>Phosphoglycerate kinase (PGK) is a X-linked enzyme that plays a key role in the glycolytic pathway. Twelve different variants have already been reported...
  31. ncbi Identification of new mutations in two phosphoglycerate kinase (PGK) variants expressing different clinical syndromes: PGK Créteil and PGK Amiens
    M Cohen-Solal
    Unité INSERM U91, Hopital Henri Mondor, Creteil, France
    Blood 84:898-903. 1994
    b>Phosphoglycerate kinase (PGK) deficiency is generally associated with chronic hemolytic anemia, although it can be accompanied by either mental retardation or muscular disease...
  32. ncbi Retarded and aberrant splicings caused by single exon mutation in a phosphoglycerate kinase variant
    T Ookawara
    Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Arch Biochem Biophys 327:35-40. 1996
    The molecular abnormality of a phosphoglycerate kinase variant which was associated with severe tissue enzyme deficiency and episodes of muscle contractions and myoglobinuria was examined...
  33. doi Substrate binding modifies the hinge bending characteristics of human 3-phosphoglycerate kinase: a molecular dynamics study
    Zoltan Palmai
    Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Hungary
    Proteins 77:319-29. 2009
    ..At the same time, ligand binding results in an enhanced correlation of internal domain motions...
  34. ncbi Molecular abnormality of a phosphoglycerate kinase variant (PGK-Alabama)
    A Yoshida
    Department of Biochemical Genetics, Beckman Institute of the City of Hope, Duarte, California, USA
    Blood Cells Mol Dis 21:179-81. 1995
    The molecular abnormality of a phosphoglycerate kinase variant associated with severe red cell enzyme deficiency ( about 4% of normal) and episodes of hemolysis with jaundice was examined...
  35. ncbi Variation for X chromosome expression in mice detected by electrophoresis of phosphoglycerate kinase
    J D West
    Genet Res 32:91-102. 1978
  36. doi Enzymatic and metabolic characterization of the phosphoglycerate kinase deficiency associated with chronic hemolytic anemia caused by the PGK-Barcelona mutation
    María José Ramírez-Bajo
    Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Spain
    Blood Cells Mol Dis 46:206-11. 2011
    Recently, we reported a new mutation of phosphoglycerate kinase (PGK), called PGK-Barcelona, which causes chronic hemolytic anemia associated with progressive neurological impairment...
  37. ncbi Two proliferation-related proteins, TYMS and PGK1, could be new cytotoxic T lymphocyte-directed tumor-associated antigens of HLA-A2+ colon cancer
    Shigeki Shichijo
    Department of Immunology, Kurume University School of Medicine, Research Center of Innovative Cancer Therapy of the 21st Century COE Program for Medical Science, Kurume University, Kurume, Fukuoka, Japan
    Clin Cancer Res 10:5828-36. 2004
    ..The purpose of this work was to provide a scientific basis for specific immunotherapy of colon cancer...
  38. ncbi Molecular abnormalities of a phosphoglycerate kinase variant generated by spontaneous mutation
    M Maeda
    Department of Biochemical Genetics, Beckman Research Institute, City of Hope, Duarte, CA 91010
    Blood 79:2759-62. 1992
    A new case of X chromosome-linked phosphoglycerate kinase (PGK) abnormality is described. The male proband was mentally retarded, had behavior disorders, and displayed episodes of hemolytic anemia...
  39. ncbi Altered expression of PGK1 in a family with phosphoglycerate kinase deficiency
    Eva K Svaasand
    Department of Pathology and Medical Genetics, St Olav Hospital, Trondheim, Norway
    Muscle Nerve 36:679-84. 2007
    The X-linked recessive disease phosphoglycerate kinase (PGK) deficiency is caused by altered expression of the PGK1 enzyme, which causes muscle stiffness, hemolytic anemia, and mental retardation...
  40. ncbi Gene expression and clonality analysis of the androgen receptor and phosphoglycerate kinase genes in polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma
    En Hua Wang
    Department of Pathology, College of Basic Medical Sciences, China Medical University, and First Affiliated Hospital of China Medical University, Shenyang, China
    Mod Pathol 20:1208-15. 2007
    ..with the methylation-sensitive enzymes HhaI or HpaII, followed by nested PCR of the androgen receptor and phosphoglycerate kinase genes. Samples with polymorphisms were identified and a clonality analysis was performed...
  41. ncbi A single amino acid substitution (157 Gly----Val) in a phosphoglycerate kinase variant (PGK Shizuoka) associated with chronic hemolysis and myoglobinuria
    H Fujii
    Department of Blood Transfusion Medicine, Tokyo Women s Medical College, Japan
    Blood 79:1582-5. 1992
    We have determined a single amino acid substitution in a new phosphoglycerate kinase (PGK) variant, PGK Shizuoka, associated with chronic hemolysis and myoglobinuria...
  42. ncbi A novel missense mutation (1060G --> C) in the phosphoglycerate kinase gene in a Japanese boy with chronic haemolytic anaemia, developmental delay and rhabdomyolysis
    Akira Morimoto
    Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan
    Br J Haematol 122:1009-13. 2003
    We report the case of a 3-year-old Japanese boy with phosphoglycerate kinase 1 (PGK1) deficiency (Online Mendelian Inheritance in Man entry 311800)...
  43. doi Native and unfolded states of phosphoglycerate kinase studied by single-molecule FRET
    Tobias Rosenkranz
    Research Centre Julich, ISB 2 Molecular Biophysics, 52425 Julich, Germany
    Chemphyschem 12:704-10. 2011
    Single-molecule Förster resonance energy transfer (FRET) measurements with phosphoglycerate kinase from yeast were performed at different concentrations of guanidine hydrochloride...
  44. ncbi Molecular defect of a phosphoglycerate kinase variant (PGK-Matsue) associated with hemolytic anemia: Leu----Pro substitution caused by T/A----C/G transition in exon 3
    M Maeda
    Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, CA 91010
    Blood 77:1348-52. 1991
    We have identified the mutation in a phosphoglycerate kinase variant (PGK-Matsue) associated with severe enzyme deficiency, congenital nonspherocytic hemolytic anemia, and mental disorders...
  45. ncbi Intronic mutation in the PGK1 gene may cause recurrent myoglobinuria by aberrant splicing
    Kentaro Shirakawa
    First Department of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Neurology 66:925-7. 2006
    A 33-year-old man with mental retardation and recurrent myoglobinuria demonstrated a deficiency in the phosphoglycerate kinase 1 (PGK1) activity of his muscles and erythrocytes...
  46. ncbi The identification of a recurrent phosphoglycerate kinase mutation associated with chronic haemolytic anaemia and neurological dysfunction in a family from USA
    Jonathan M Flanagan
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, MEM 215, 10550 Torrey Pines, La Jolla, CA, USA
    Br J Haematol 134:233-7. 2006
    b>Phosphoglycerate kinase (PGK) deficiency is a rare X-linked disease that is characterised by mild to severe haemolytic anaemia, rhabdomyolysis, and variable defects in the central nervous system...
  47. ncbi A single amino acid substitution (Asp leads to Asn) in a phosphoglycerate kinase variant (PGK München) associated with enzyme deficiency
    H Fujii
    J Biol Chem 255:6421-3. 1980
    The structural abnormality of the phosphoglycerate kinase variant, PGK München, associated with red cell enzyme deficiency and heat instability, was elucidated by a microscale peptide-mapping method...
  48. pmc Use of cultured lymphoblastoid cells for the study of abnormal enzymes: molecular abnormality of a phosphoglycerate kinase variant associated with hemolytic anemia
    H Fujii
    Proc Natl Acad Sci U S A 78:2587-90. 1981
    A phosphoglycerate kinase (PGKase: ATP:3-phosphoglycerate 1-phosphotransferase, EC 2.7.2.3; X chromosome-linked) variant, PGKase-Tokyo, is associated with enzyme deficiency, nonspherocytic hemolytic anemia, and neurological disturbances...
  49. pmc Molecular abnormality of phosphoglycerate kinase-Uppsala associated with chronic nonspherocytic hemolytic anemia
    H Fujii
    Proc Natl Acad Sci U S A 77:5461-5. 1980
    Inherited deficiency of phosphoglycerate kinase (PGK; ATP:3-phosphoglycerate 1-phosphotransferase, EC 2.7.2.3) is associated with chronic nonspherocytic hemolytic anemia and mental disorders in man...
  50. ncbi Enzymatic activity of immobilized yeast phosphoglycerate kinase
    Cedric Hurth
    Centre de Physique Moleculaire Optique et Hertzienne, 33600 Pessac, France
    Biosens Bioelectron 22:2449-55. 2007
    This work reports the first evidence that recombinant yeast phosphoglycerate kinase (PGK) is still significantly active when immobilized on glass and muscovite mica...
  51. ncbi Denatured states of yeast phosphoglycerate kinase
    G Damaschun
    Institut für Biologie der Humboldt Universität zu Berlin, Germany
    Biochemistry (Mosc) 63:259-75. 1998
    ..Acid-denatured, cold-denatured, and 6 M guanidine hydrochloride (GuHCl) denatured yeast phosphoglycerate kinase (PGK) are ensembles of flexible unfolded molecules with rapidly interconverting structures of the ..
  52. ncbi Immunoelectron microscopic analysis of the intracellular distribution of primer recognition proteins, annexin 2 and phosphoglycerate kinase, in normal and transformed cells
    K D Kumble
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 99:751-8. 1991
    ..We have previously reported that the PRP consist of a complex of two proteins identified as 3-phosphoglycerate kinase (PGK) and the protein-tyrosine kinase substrate, annexin 2 monomer...
  53. doi Effect of hypoxia on the expression of phosphoglycerate kinase and antitumor activity of troxacitabine and gemcitabine in non-small cell lung carcinoma
    Wing Lam
    Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510, USA
    Mol Cancer Ther 8:415-23. 2009
    ..The cytotoxicity of L-OddC is dependent on its phosphorylation to L-OddCTP by phosphoglycerate kinase (PGK) and its subsequent addition into nuclear DNA...
  54. doi Structural tightening and interdomain communication in the catalytic cycle of phosphoglycerate kinase
    James P Marston
    Department of Molecular Biology and Biotechnology, Firth Court, The University of Sheffield, Western Bank, Sheffield, UK
    J Mol Biol 396:345-60. 2010
    Changes in amide-NH chemical shift and hydrogen exchange rates as phosphoglycerate kinase progresses through its catalytic cycle have been measured to assess whether they correlate with changes in hydrogen bonding within the protein...
  55. doi Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expression
    Ming Yi Ho
    Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Department of Education and Research, Taipei City Hospital, Taipei, Taiwan
    Cancer Sci 101:2411-6. 2010
    b>Phosphoglycerate kinase 1 (PGK-1) is a multifunctional protein that is involved in the glycolytic pathway and the generation of the angiogenesis inhibitor angiostatin...
  56. doi A sandwich ELISA for phosphoglycerate kinase
    Weilin Zhao
    Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
    J Immunoassay Immunochem 29:220-33. 2008
    b>Phosphoglycerate kinase (PGK1) is a key enzyme in glycolysis that can also be released from certain cells...
  57. doi Blocking Fasciola hepatica's energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinase
    Slawomir Jaros
    Department of Molecular Biology, Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland
    Vet Parasitol 172:229-37. 2010
    ..This paper is focused on first vaccination trials based on a new vaccine candidate antigen, F. hepatica phosphoglycerate kinase (FhPGK) performed on a rat model...
  58. pmc Entry of Spiroplasma citri into Circulifer haematoceps cells involves interaction between spiroplasma phosphoglycerate kinase and leafhopper actin
    Fabien Labroussaa
    UMR 1090 Génomique Diversité Pouvoir Pathogène, INRA, Universite Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP 81, F 33883 Villenave d Ornon, France
    Appl Environ Microbiol 76:1879-86. 2010
    ..actin-binding protein of 44 kDa was isolated by affinity chromatography and identified by LC-MS/MS as phosphoglycerate kinase (PGK)...
  59. doi Processing of a phosphoglycerate kinase reporter mRNA in Trypanosoma brucei is not coupled to transcription by RNA polymerase II
    Mhairi Stewart
    Zentrum für Molekularbiologie der Universität Heidelberg, ZMBH DKFZ Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Mol Biochem Parasitol 172:99-106. 2010
    ..There was therefore no evidence for recruitment of the 3'-splicing apparatus by the RNA polymerase. Abundant transcripts transcribed from the rRNA locus by T7 RNA polymerase were somewhat less efficiently processed...
  60. pmc Foamy-virus-mediated gene transfer to canine repopulating cells
    Hans Peter Kiem
    Clinical Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N D1 100, PO Box 19024, Seattle, WA 98109 1024, USA
    Blood 109:65-70. 2007
    ..These data suggest that FV vectors should be useful for stem-cell gene therapy, particularly for applications in which short transduction protocols are critical...
  61. pmc Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding
    Apratim Dhar
    Department of Chemistry, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 107:17586-91. 2010
    ..show how macromolecular crowding dramatically affects the structure, function, and folding landscape of phosphoglycerate kinase (PGK)...
  62. ncbi Human testis-specific PGK gene lacks introns and possesses characteristics of a processed gene
    J R McCarrey
    Nature 326:501-5. 1987
    b>Phosphoglycerate kinase (PGK) (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is a metabolic enzyme functioning in the Embden-Meyerhof pathway that converts glucose (or fructose) to pyruvate...
  63. ncbi Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3'UTR
    Mingang Xu
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104 6080, USA
    Biol Reprod 76:1025-33. 2007
    The mRNA that encodes the testis-specific protein phosphoglycerate kinase (PGK2) is a long-lived mRNA that is transcribed in meiotic and postmeiotic male germ cells...
  64. ncbi The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cells
    J K Vishwanatha
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 101:25-34. 1992
    ..have previously identified the protein-tyrosine kinase substrate annexin II, and the glycolytic enzyme 3-phosphoglycerate kinase as components of PRP...
  65. doi Promoter-dependent EGFP expression during embryonic stem cell propagation and differentiation
    Rong Wang
    Key Laboratory of Stem Cell Biology of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Stem Cells Dev 17:279-89. 2008
    ..the transcriptional activities of three ubiquitous promoters, elongation factor-1alpha (EF1alpha), phosphoglycerate kinase-1 (PGK), and cytomegalovirus (CMV), during propagation and differentiation of mouse (m) ES cells by using ..
  66. ncbi Testis specific phosphoglycerate kinase B in mouse
    J L Vandeberg
    J Exp Zool 198:231-40. 1976
    Mammals possess two genetically distinct forms of phosphoglycerate kinase (PGK)...
  67. ncbi Retrovirus silencing, variegation, extinction, and memory are controlled by a dynamic interplay of multiple epigenetic modifications
    Shuyuan Yao
    Developmental Biology Program, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8
    Mol Ther 10:27-36. 2004
    ..A molecular mechanism of competitive H1 and MeCP2 binding may account for this epigenetic interplay, and a model for variegation is discussed...
  68. ncbi Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis
    S J Hong
    Department of Parasitology and Pathology, Chung Ang University College of Medicine, Tongjak gu, 156 756, Seoul, South Korea
    Mol Biochem Parasitol 108:207-16. 2000
    ..b>Phosphoglycerate kinase (PGK), a glycolytic enzyme, found in many parasites, has been identified as one of the candidate ..
  69. ncbi Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18
    M Bentahir
    Laboratoire de Biochimie, Institut de Chimie B6, Universite de Liege, Sart Tilman, B 4000 Liege, Belgium
    J Biol Chem 275:11147-53. 2000
    The gene encoding the phosphoglycerate kinase (PGK) from the Antarctic Pseudomonas sp. TACII18 has been cloned and found to be inserted between the genes encoding for glyceraldhyde-3-phosphate dehydrogenase and fructose aldolase...
  70. doi Myopathy and parkinsonism in phosphoglycerate kinase deficiency
    Evangelia Sotiriou
    Department of Neurology, Columbia University Medical Center, 3 313 Russ Berrie Medical Science Pavilion, 1150 St Nicholas Avenue, New York, New York 10032, USA
    Muscle Nerve 41:707-10. 2010
    ..b>Phosphoglycerate kinase (PGK) activity was markedly decreased in muscle, and molecular analysis of the PGK1 gene identified the p...
  71. pmc Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements
    Luis Quijada
    ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Nucleic Acids Res 30:4414-24. 2002
    ..We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA ..
  72. ncbi Protein conformer selection by sequence-dependent packing contacts in crystals of 3-phosphoglycerate kinase
    Zoltán Kovári
    Department of Theoretical Chemistry, Eotvos Lorand University, Budapest, Hungary
    Proteins 55:198-209. 2004
    In several crystal structures of 3-phosphoglycerate kinase (PGK), the two domains occupy different relative positions...
  73. pmc Functional analysis of various promoters in lentiviral vectors at different stages of in vitro differentiation of mouse embryonic stem cells
    Sunghoi Hong
    Molecular Neurobiology Laboratory, McLean Hospital, Harvard Medical School, Belmont, Massachusetts, USA
    Mol Ther 15:1630-9. 2007
    ..promoter (consisting of the CMV immediate early enhancer and the chicken beta-actin promoter), the human phosphoglycerate kinase 1 (PGK) promoter, the murine stem cell virus (MSCV) long terminal repeat (LTR), or the gibbon ape ..
  74. pmc Structure of the human phosphoglycerate kinase gene and the intron-mediated evolution and dispersal of the nucleotide-binding domain
    A M Michelson
    Proc Natl Acad Sci U S A 82:6965-9. 1985
    The human X-linked phosphoglycerate kinase (PGK) gene, which is expressed in all somatic cells, was cloned and its structure was determined. The gene is interrupted by 10 introns and spans 23 kilobases...
  75. ncbi Crystallographic and thiol-reactivity studies on the complex of pig muscle phosphoglycerate kinase with ATP analogues: correlation between nucleotide binding mode and helix flexibility
    Zoltán Kovári
    Eotvos Lorand University, Department of Theoretical Chemistry, H 1518, Budapest 112, P O Box 32, Hungary
    Biochemistry 41:8796-806. 2002
    Crystal structure of the ternary complex of pig muscle phosphoglycerate kinase (PGK) with the substrate 3-phosphoglycerate (3-PG) and the Mg(2+) complex of beta,gamma-methylene-adenosine-5'-triphosphate (AMP-PCP), a nonreactive analogue ..
  76. ncbi Overexpression of human phosphoglycerate kinase 1 (PGK1) induces a multidrug resistance phenotype
    Z Duan
    Department of Hematology Oncology, Massachusetts General Hospital, Boston 02114, USA
    Anticancer Res 22:1933-41. 2002
    ..Multidrug resistance is a significant barrier to the development of successful cancer treatment. To identify genetic alterations that are directly involved in paclitaxel resistance, a functional cloning strategy was developed...
  77. ncbi Characterization of the molecular mechanisms involved in the differential production of erythrose-4-phosphate dehydrogenase, 3-phosphoglycerate kinase and class II fructose-1,6-bisphosphate aldolase in Escherichia coli
    Vincent Bardey
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, UMR 7567 CNRS UHP Nancy I, Faculte des Sciences et Techniques, BP 239, 54506 Vandoeuvre les Nancy, Cedex, France
    Mol Microbiol 57:1265-87. 2005
    A gapA-pgk gene tandem coding the glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate kinase, is most frequently found in bacteria...
  78. ncbi Control and regulation of gene expression: quantitative analysis of the expression of phosphoglycerate kinase in bloodstream form Trypanosoma brucei
    Jurgen R Haanstra
    Vrije Universiteit, BioCentrum Amsterdam, De Boelelaan 1085, Amsterdam, The Netherlands
    J Biol Chem 283:2495-507. 2008
    Isoenzymes of phosphoglycerate kinase in Trypanosoma brucei are differentially expressed in its two main life stages...
  79. ncbi Regulation of urokinase receptor expression by phosphoglycerate kinase
    Sreerama Shetty
    Department of Specialty Care Services, The University of Texas Health Center at Tyler, Tyler, TX 75708, USA
    Am J Respir Cell Mol Biol 31:100-6. 2004
    ..We purified this protein from human bronchial epithelial (Beas2B) cells and identified it as phosphoglycerate kinase (PGK)...
  80. pmc In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis
    Hirotaka Yoshioka
    Department of Biology, University of Texas at San Antonio, San Antonio, Texas 78249, USA
    Mol Cell Biol 27:7871-85. 2007
    ..of the testis-specific Pgk2 gene is selectively activated in primary spermatocytes to provide a source of phosphoglycerate kinase that is critical to normal motility and fertility of mammalian spermatozoa...
  81. ncbi Structure of a circularly permuted phosphoglycerate kinase
    Pierre Tougard
    Laboratoire de Modélisation et d Ingénierie des Protéines, UMR 8619, Universite de Paris Sud, Batiment 430, F 91405 Orsay cedex, France
    Acta Crystallogr D Biol Crystallogr 58:2018-23. 2002
    ..The effect of the mutation on the functional properties is more easily accounted for by a restriction of hinge-bending motion than by structural changes in the protein...
  82. ncbi Activity analysis of housekeeping promoters using self-inactivating lentiviral vector delivery into the mouse retina
    C Kostic
    Oculogenetic Institute of Neuroscience Unit, Jules Gonin Eye Hospital, Lausanne, Switzerland
    Gene Ther 10:818-21. 2003
    ..specific retinal cell types, we have analyzed the expression pattern of three different promoters (mouse phosphoglycerate kinase 1 (PGK), elongation factor-1 (EFS), rhodopsin (Rho)) in newborn and adult DBA/2 mice retinas using self-..
  83. ncbi Role of phosphate chain mobility of MgATP in completing the 3-phosphoglycerate kinase catalytic site: binding, kinetic, and crystallographic studies with ATP and MgATP
    Beáta Flachner
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, P O Box 7, H 1518 Budapest, Hungary
    Biochemistry 43:3436-49. 2004
    The complexes of pig muscle 3-phosphoglycerate kinase with the substrate MgATP and with the nonsubstrate Mg(2+)-free ATP have been characterized by binding, kinetic, and crystallographic studies...
  84. ncbi Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylases
    Elise B Daly
    Centre for and Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, NSW 2031, Australia
    Biochim Biophys Acta 1691:17-22. 2004
    Solid tumour cells employ glycolytic enzymes including phosphoglycerate kinase (PGK) to make ATP when their supply of oxygen is limiting...
  85. ncbi Overexpression and elevated serum levels of phosphoglycerate kinase 1 in pancreatic ductal adenocarcinoma
    Tsann Long Hwang
    Department of Surgery, Chang Gung Memorial Hospital, Chang Gung University, Tao Yuan, Taiwan
    Proteomics 6:2259-72. 2006
    ..One of them was identified as phosphoglycerate kinase (PGK) 1, a secretable glycolytic enzyme known to participate in angiogenesis...
  86. ncbi Substrate-assisted movement of the catalytic Lys 215 during domain closure: site-directed mutagenesis studies of human 3-phosphoglycerate kinase
    Beáta Flachner
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    Biochemistry 44:16853-65. 2005
    3-Phosphoglycerate kinase (PGK) is a two-domain hinge-bending enzyme...
  87. ncbi Differential transcription of Pgk genes during spermatogenesis in the mouse
    J R McCarrey
    Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78248
    Dev Biol 154:160-8. 1992
    ..cell-type specificity of these mRNAs parallel that previously reported for the two protein isozymes of phosphoglycerate kinase (PGK) encoded by these two genes...
  88. ncbi Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei
    Claudia Colasante
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Mol Biochem Parasitol 151:193-204. 2007
    In Trypanosoma brucei, the PGKB and PGKC genes-encoding phosphoglycerate kinase are co-transcribed as part of a polycistronic RNA...
  89. ncbi Effect of dietary carbohydrates and ethanol on expression of genes encoding sn-glycerol-3-phosphate dehydrogenase, aldolase, and phosphoglycerate kinase in Drosophila larvae
    J L Lissemore
    Department of Biology, Syracuse University, New York 13244 1220
    Biochem Genet 28:615-30. 1990
    ..on expression of the genes encoding glycerol-3-phosphate dehydrogenase (GPDH), aldolase (ALD), and phosphoglycerate kinase (PGK) in D. melanogaster larvae...
  90. ncbi Chloroplast phosphoglycerate kinase from Euglena gracilis: endosymbiotic gene replacement going against the tide
    Ulrich Nowitzki
    Institute of Botany III, Heinrich Heine University Dusseldorf, Germany
    Eur J Biochem 271:4123-31. 2004
    Two chloroplast phosphoglycerate kinase isoforms from the photosynthetic flagellate Euglena gracilis were purified to homogeneity, partially sequenced, and subsequently cDNAs encoding phosphoglycerate kinase isoenzymes from both the ..
  91. ncbi Broad specificity of human phosphoglycerate kinase for antiviral nucleoside analogs
    Sarah Gallois-Montbrun
    Unité de Régulation Enzymatique des Activités Cellulaires, CNRS URA 2185, Institut Pasteur, 75724 Paris Cedex 15, France
    Biochem Pharmacol 68:1749-56. 2004
    ..Human phosphoglycerate kinase (hPGK) was recently reported to participate in the last step of phosphorylation of cytidine L-nucleotide ..
  92. pmc Transcriptional regulatory regions of testis-specific PGK2 defined in transgenic mice
    M O Robinson
    Division of Biology, California Institute of Technology, Pasadena 91125
    Proc Natl Acad Sci U S A 86:8437-41. 1989
    The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells...
  93. pmc The testis-specific phosphoglycerate kinase gene pgk-2 is a recruited retroposon
    P H Boer
    Department of Medicine, University of Ottawa, Canada
    Mol Cell Biol 7:3107-12. 1987
    In both humans and mice, two genes encode phosphoglycerate kinase, a key enzyme in the glycolytic pathway. The pgk-1 gene is expressed in all somatic cells, is located on the X chromosome, and contains 10 introns...
  94. ncbi Biochemical characterization and crystallization of recombinant 3-phosphoglycerate kinase of Plasmodium falciparum
    Biswajit Pal
    Department of Chemistry, University of Alabama at Birmingham, 901 14th Street South, University of Alabama at Birmingham, AL 35294, USA
    Biochim Biophys Acta 1699:277-80. 2004
    ..3-Phosphoglycerate kinase (EC 2.7.2...
  95. ncbi Phylogeny of Firmicutes with special reference to Mycoplasma (Mollicutes) as inferred from phosphoglycerate kinase amino acid sequence data
    Matthias Wolf
    University of Wurzburg, Department of Bioinformatics, Biocenter, Am Hubland, 97074 Wurzburg, Germany
    Int J Syst Evol Microbiol 54:871-5. 2004
    The phylogenetic position of the Mollicutes has been re-examined by using phosphoglycerate kinase (Pgk) amino acid sequences. Hitherto unpublished sequences from Mycoplasma mycoides subsp...
  96. ncbi Phosphorylation of pyrimidine L-deoxynucleoside analog diphosphates. Kinetics of phosphorylation and dephosphorylation of nucleoside analog diphosphates and triphosphates by 3-phosphoglycerate kinase
    Preethi Krishnan
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 277:31593-600. 2002
    ..We recently reported that in the last step, L-deoxynucleoside analog diphosphates are phosphorylated by 3-phosphoglycerate kinase (PGK)...
  97. ncbi Conversion of yeast phosphoglycerate kinase into amyloid-like structure
    G Damaschun
    Humboldt Universitat zu Berlin, Institut fur Biologie, Berlin, Germany
    Proteins 39:204-11. 2000
    Yeast phosphoglycerate kinase is a structurally well-characterized enzyme consisting of 415 amino acids without disulfide bonds...
  98. ncbi The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli
    C E Jones
    Department of Biochemistry and Molecular Biology, University College, London, England
    Eur J Biochem 233:800-8. 1995
    The overlapping genes encoding phosphoglycerate kinase (PGK) and glyceraldehyde-3-phosphate dehydrogenase (GraP-DH) from the hyperthermophilic archaeon Sulfolobus solfataricus have been cloned and sequenced...
  99. pmc The identification of phosphoglycerate kinase-1 and histone H4 autoantibodies in pancreatic cancer patient serum using a natural protein microarray
    Tasneem H Patwa
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 0656, USA
    Electrophoresis 30:2215-26. 2009
    ..b>Phosphoglycerate kinase-1 and histone H4 were noted to elicit a significant differential humoral response in cancer sera compared ..
  100. ncbi The structure of a thermally stable 3-phosphoglycerate kinase and a comparison with its mesophilic equivalent
    G J Davies
    Department of Biochemistry, School of Medical Sciences, University of Bristol, England
    Proteins 15:283-9. 1993
    The structure of the phosphoglycerate kinase (PGK) from Bacillus stearothermophilus, a moderate thermophile, has been determined and compared with that of its mesophilic equivalent from yeast...
  101. doi Differences in the transient kinetics of the binding of D-ADP and its mirror image L-ADP to human 3-phosphoglycerate kinase revealed by the presence of 3-phosphoglycerate
    Claire Gondeau
    Centre d tudes d agents Pathogènes et Biotechnologies pour la Santé CPBS, UMR 5236, CNRS Université Montpellier 1 Université Montpellier 2, Montpellier Cedex 2, France
    Biochemistry 47:3462-73. 2008
    ..The last step of the cascade may be catalyzed by 3-phosphoglycerate kinase (PGK), an enzyme that has low specificity for nucleoside diphosphate (NDP): NDP + 1,3-bisphosphoglycerate ..

Research Grants65

  1. Glycolysis and Glutamate Storage into Synaptic Vesicles
    Tetsufumi Ueda; Fiscal Year: 2010
    ..We have recently provided evidence that glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase, glycolytic enzymes responsible for production of ATP, are associated with synaptic vesicles and that ..
  2. REGULATION OF GLYCOSOMAL BIOGENESIS IN T. BRUCEI
    C Wang; Fiscal Year: 1992
    ..The chemically synthesized C-terminal peptide of glycosomal 3-phosphoglycerate kinase (PGK) postulated as essential for PGK insertion, was, however, without effect...
  3. Glycolysis and Glutamate Storage into Synaptic Vesicles
    Tetsufumi Ueda; Fiscal Year: 2007
    ..We have recently provided evidence that glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase, glycolytic enzymes responsible for production of ATP, are associated with synaptic vesicles and that ..
  4. SPECTROSCOPIC STUDIES OF ENZYME-SUBSTRATE COMPLEXES
    George Reed; Fiscal Year: 1993
    ..structures of enzyme metal-substrate or inhibitor complexes with pyruvate kinase, creatine kinase, 3-phosphoglycerate kinase, and enolase...
  5. NMR STUDIES OF METABOLISM AND REGULATION IN CELLS
    Robert Shulman; Fiscal Year: 1990
    ..from the mitochondrial ATPase, the plasma membrane ATPase, and glyceraldehyde 3-phosphate dehydrogenase/phosphoglycerate kinase coupled reaction...
  6. MOLECULAR ANALYSIS OF THE TRYPANOSOME GLYCOSOME
    Marilyn Parsons; Fiscal Year: 1999
    ..Gene knockout and gene replacement studies will examine the role of a phosphoglycerate kinase found only in the glycosome of Kinetoplastida. Glycosome-deficient mutants of T...
  7. NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDS
    Yung Chi Cheng; Fiscal Year: 2007
    ..2. To examine the role of Hypoxia: a. in regulating phosphoglycerate kinase (PGK), which is responsible for the phosphorylation of L- OddCDP to L-OddCTP. b...
  8. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 1993
    ..Two constitutively expressed genes, the mini-exon donor RNA (medRNA) and phosphoglycerate kinase (PGK B and C) genes probably have essential roles in trypanosomes...
  9. MOLECULAR ANALYSIS OF THE TRYPANOSOME GLYCOSOME
    Marilyn Parsons; Fiscal Year: 1993
    ..We have demonstrated translational control of expression of glycosomal phosphoglycerate kinase. The molecular mechanisms of this control will be analyzed...
  10. NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDS
    Yung Chi Cheng; Fiscal Year: 2007
    ..2. To examine the role of Hypoxia: a. in regulating phosphoglycerate kinase (PGK), which is responsible for the phosphorylation of L- OddCDP to L-OddCTP. b...