phosphoglycerate kinase

Summary

Summary: An enzyme catalyzing the transfer of a phosphate group from 3-phospho-D-glycerate in the presence of ATP to yield 3-phospho-D-glyceroyl phosphate and ADP. EC 2.7.2.3.

Top Publications

  1. pmc The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice
    Cyntia G M C Trant
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil
    Infect Immun 78:2283-91. 2010
  2. pmc Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat
    Shaoxing Huang
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 99:8133-8. 2002
  3. ncbi Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis
    Matthew J Cliff
    The Krebs Institute and The Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Am Chem Soc 132:6507-16. 2010
  4. ncbi Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinase
    Judit Szabo
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, PO Box 7, Hungary, Germany
    Biochemistry 47:6735-44. 2008
  5. ncbi Physiological promoters reduce the genotoxic risk of integrating gene vectors
    Daniela Zychlinski
    Department of Experimental Hematology, Hannover Medical School, Hannover, Germany
    Mol Ther 16:718-25. 2008
  6. ncbi The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cells
    J K Vishwanatha
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 101:25-34. 1992
  7. pmc Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression
    Jianhua Wang
    Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China
    Cancer Res 70:471-80. 2010
  8. ncbi Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3'UTR
    Mingang Xu
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104 6080, USA
    Biol Reprod 76:1025-33. 2007
  9. pmc Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding
    Apratim Dhar
    Department of Chemistry, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 107:17586-91. 2010
  10. pmc A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase
    Louiza Zerrad
    Structural Biology Group, European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F 38043 Grenoble, France
    J Biol Chem 286:14040-8. 2011

Research Grants

Detail Information

Publications287 found, 100 shown here

  1. pmc The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice
    Cyntia G M C Trant
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Av Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil
    Infect Immun 78:2283-91. 2010
    ..Herein, we identified the gene encoding the phosphoglycerate kinase (PGK) of B. abortus strain 2308...
  2. pmc Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat
    Shaoxing Huang
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 99:8133-8. 2002
    ..We analyzed Acc-1 (plastid acetyl-CoA carboxylase) and Pgk-1 (plastid 3-phosphoglycerate kinase) genes to determine phylogenetic relationships among Triticum and Aegilops species of the wheat lineage ..
  3. ncbi Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis
    Matthew J Cliff
    The Krebs Institute and The Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Am Chem Soc 132:6507-16. 2010
    Transition state analogue (TSA) complexes formed by phosphoglycerate kinase (PGK) have been used to test the hypothesis that balancing of charge within the transition state dominates enzyme-catalyzed phosphoryl transfer...
  4. ncbi Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinase
    Judit Szabo
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, PO Box 7, Hungary, Germany
    Biochemistry 47:6735-44. 2008
    3-Phosphoglycerate kinase is a hinge-bending enzyme with substrate-assisted domain closure. However, the closure mechanism has not been described in terms of structural details...
  5. ncbi Physiological promoters reduce the genotoxic risk of integrating gene vectors
    Daniela Zychlinski
    Department of Experimental Hematology, Hannover Medical School, Hannover, Germany
    Mol Ther 16:718-25. 2008
    ..promoters than did cellular promoters derived from human genes, elongation factor-1alpha (EF1alpha) and phosphoglycerate kinase (PGK). Self-inactivating (SIN) vector design reduced but did not abolish enhancer interactions...
  6. ncbi The role of primer recognition proteins in DNA replication: association with nuclear matrix in HeLa cells
    J K Vishwanatha
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 101:25-34. 1992
    ..have previously identified the protein-tyrosine kinase substrate annexin II, and the glycolytic enzyme 3-phosphoglycerate kinase as components of PRP...
  7. pmc Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression
    Jianhua Wang
    Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China
    Cancer Res 70:471-80. 2010
    ..microdissection and cDNA microarray analysis of CAFs in prostate tumors revealed strong upregulation of phosphoglycerate kinase-1 (PGK1), an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway and is directly ..
  8. ncbi Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3'UTR
    Mingang Xu
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104 6080, USA
    Biol Reprod 76:1025-33. 2007
    The mRNA that encodes the testis-specific protein phosphoglycerate kinase (PGK2) is a long-lived mRNA that is transcribed in meiotic and postmeiotic male germ cells...
  9. pmc Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding
    Apratim Dhar
    Department of Chemistry, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 107:17586-91. 2010
    ..show how macromolecular crowding dramatically affects the structure, function, and folding landscape of phosphoglycerate kinase (PGK)...
  10. pmc A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase
    Louiza Zerrad
    Structural Biology Group, European Synchrotron Radiation Facility, 6 rue Jules Horowitz, F 38043 Grenoble, France
    J Biol Chem 286:14040-8. 2011
    b>Phosphoglycerate kinase (PGK) is the enzyme responsible for the first ATP-generating step of glycolysis and has been implicated extensively in oncogenesis and its development...
  11. pmc Molecular basis for the lack of enantioselectivity of human 3-phosphoglycerate kinase
    C Gondeau
    Centre d Etudes d Agents Pathogenes et Biotechnologies pour la Sante, UMR 5236, CNRS Universités Montpellier 1 et 2, Institut de Biologie, 4 Bd Henri IV, CS69033, 34965 Montpellier cedex 2, France
    Nucleic Acids Res 36:3620-9. 2008
    ..to reveal the molecular basis for the low enantioselectivity and the broad specificity of human 3-phosphoglycerate kinase (hPGK), an enzyme responsible for the last step of phosphorylation of many nucleotide derivatives...
  12. ncbi Promoter-dependent EGFP expression during embryonic stem cell propagation and differentiation
    Rong Wang
    Key Laboratory of Stem Cell Biology of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Stem Cells Dev 17:279-89. 2008
    ..the transcriptional activities of three ubiquitous promoters, elongation factor-1alpha (EF1alpha), phosphoglycerate kinase-1 (PGK), and cytomegalovirus (CMV), during propagation and differentiation of mouse (m) ES cells by using ..
  13. ncbi Binding of group B streptococcal phosphoglycerate kinase to plasminogen and actin
    Tyler J Boone
    The Department of Laboratory Medicine and Pathology, The University of Alberta, Edmonton, Alberta, Canada
    Microb Pathog 51:255-61. 2011
    The glycolytic enzyme, phosphoglycerate kinase (PGK) of group B streptococci (GBS), has previously been identified as expressed on the GBS cell surface...
  14. ncbi Crystal structure of Plasmodium falciparum phosphoglycerate kinase: evidence for anion binding in the basic patch
    Craig D Smith
    University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochem Biophys Res Commun 412:203-6. 2011
    3-Phosphoglycerate kinase (EC 2.7.2.3) is a key enzyme in the glycolytic pathway and catalyzes an important phosphorylation step leading to the production of ATP...
  15. pmc Evolutionary dynamics of the Pgk1 gene in the polyploid genus Kengyilia (Triticeae: Poaceae) and its diploid relatives
    Xing Fan
    Triticeae Research Institute, Sichuan Agricultural University, Sichuan, People s Republic of China
    PLoS ONE 7:e31122. 2012
    ....
  16. ncbi Phosphoglycerate kinase 1 promoting tumor progression and metastasis in gastric cancer - detected in a tumor mouse model using positron emission tomography/magnetic resonance imaging
    Derek Zieker
    Department of General, Visceral and Transplant Surgery, Comprehensive Cancer Center, University of Tuebingen, Tuebingen, Germany
    Cell Physiol Biochem 26:147-54. 2010
    ..In previous studies we were able to show a relevant impact of increased phosphoglycerate kinase 1 expression (PGK1; a glycolytic enzyme) on invasive properties of gastric cancer in-vivo and in-vitro...
  17. pmc Phosphoglycerate kinase and triosephosphate isomerase from the hyperthermophilic bacterium Thermotoga maritima form a covalent bifunctional enzyme complex
    H Schurig
    Institut fur Biophysik und Physikalische Biochemie, Universitat Regensburg, Germany
    EMBO J 14:442-51. 1995
    b>Phosphoglycerate kinase (PGK) from the hyperthermophilic bacterium Thermotoga maritima has been purified to homogeneity. A second larger enzyme with PGK activity and identical N-terminal sequence was also found...
  18. pmc Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomerase
    B J Eikmanns
    Institut für Biotechnologie 1 des Forschungszentrums Jülich GmbH, Germany
    J Bacteriol 174:6076-86. 1992
    ..of three glycolytic genes, namely, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene gap, the 3-phosphoglycerate kinase (PGK) gene pgk, and the triosephosphate isomerase (TPI) gene tpi...
  19. ncbi Determination of clonal status of pulmonary sclerosing hemangioma with X-chromosome inactivation mosaicism and polymorphism of phosphoglycerate kinase and androgen receptor genes
    Li Gong
    The Helmholtz Sino German Research Laboratory for Cancer, Department of Pathology, Tangdu Hospital, The Fourth Military Medical University, Xi an, 710038, People s Republic of China
    Med Oncol 28:913-8. 2011
    ..of cells using laser microdissection and X-chromosomal inactivation mosaicism and polymorphism at the phosphoglycerate kinase (PGK) and androgen receptor (AR) loci in female somatic cells...
  20. pmc Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus
    Jen Wen Lin
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
    Nucleic Acids Res 35:424-32. 2007
    ..LC/MS/MS sequencing indicates that p43 is a chloroplast phosphoglycerate kinase (PGK)...
  21. ncbi Substrate-induced double sided H-bond network as a means of domain closure in 3-phosphoglycerate kinase
    Andrea Varga
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    FEBS Lett 580:2698-706. 2006
    Closure of the two domains of 3-phosphoglycerate kinase, upon substrate binding, is essential for the enzyme function...
  22. ncbi Effect of hypoxia on the expression of phosphoglycerate kinase and antitumor activity of troxacitabine and gemcitabine in non-small cell lung carcinoma
    Wing Lam
    Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510, USA
    Mol Cancer Ther 8:415-23. 2009
    ..The cytotoxicity of L-OddC is dependent on its phosphorylation to L-OddCTP by phosphoglycerate kinase (PGK) and its subsequent addition into nuclear DNA...
  23. pmc Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements
    Luis Quijada
    ZMBH, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Nucleic Acids Res 30:4414-24. 2002
    ..We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA ..
  24. ncbi Glycolysis and glutamate accumulation into synaptic vesicles. Role of glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase
    Atsushi Ikemoto
    Mental Health Research Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109 0669, USA
    J Biol Chem 278:5929-40. 2003
    ..We demonstrate that the glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate kinase (3-PGK) are enriched in synaptic vesicles, forming a functional complex, and that synaptic vesicles are ..
  25. ncbi Myopathic form of phosphoglycerate kinase (PGK) deficiency: a new case and pathogenic considerations
    Ronen Spiegel
    Department of Pediatrics, Haemek Medical Center, Rappaport School of Medicine, Afula, Israel
    Neuromuscul Disord 19:207-11. 2009
    ..b>Phosphoglycerate kinase (PGK) deficiency was documented in muscle and erythrocytes and molecular analysis of the PGK1 gene ..
  26. ncbi Stable transgene expression in human embryonic stem cells after simple chemical transfection
    Jun Liu
    Cell Reprogramming Laboratory, Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Victoria, Australia
    Mol Reprod Dev 76:580-6. 2009
    ..We demonstrated that cytomegalovirus (CMV), phosphoglycerate kinase (PGK) and human elongation factor-1alpha (EF1alpha) promoters all resulted in robust activity of a ..
  27. ncbi Hierarchic finite level energy landscape model: to describe the refolding kinetics of phosphoglycerate kinase
    Szabolcs Osváth
    Department of Biophysics and Radiation Biology, Semmelweis University, Puskin u 9, H 1088 Budapest, Hungary
    J Biol Chem 281:24375-80. 2006
    ..Refolding of yeast phosphoglycerate kinase was initiated from the guanidine-unfolded state by stopped-flow or manual mixing and monitored by ..
  28. pmc Magnetic isotope effect of magnesium in phosphoglycerate kinase phosphorylation
    Anatoly L Buchachenko
    N N Semenov Institute for Chemical Physics, Russian Academy of Sciences, Kosygin Street 4, Moscow 119991, Russia
    Proc Natl Acad Sci U S A 102:10793-6. 2005
    b>Phosphoglycerate kinase (PGK) is found to be controlled by a (25)Mg(2+)-related magnetic isotope effect...
  29. ncbi Inhibiting 3-phosphoglycerate kinase by EDTA stimulates the development of the cleavage stage mouse embryo
    M Lane
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Mol Reprod Dev 60:233-40. 2001
    ..EDTA was shown to inhibit the activity of the glycolytic enzyme, 3-phosphoglycerate kinase. Additionally, the effect of EDTA on maintaining high rates of embryo development in culture could be ..
  30. ncbi Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylases
    Elise B Daly
    Centre for and Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, NSW 2031, Australia
    Biochim Biophys Acta 1691:17-22. 2004
    Solid tumour cells employ glycolytic enzymes including phosphoglycerate kinase (PGK) to make ATP when their supply of oxygen is limiting...
  31. ncbi Molecular characterization of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastoris
    João Ricardo Moreira de Almeida
    Laboratorio de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, DF 70910 900, Brazil
    Yeast 22:725-37. 2005
    We report the cloning of the 3-phosphoglycerate kinase gene (PGK1) from the methylotrophic yeast Pichia pastoris by a PCR approach...
  32. ncbi Role of phosphate chain mobility of MgATP in completing the 3-phosphoglycerate kinase catalytic site: binding, kinetic, and crystallographic studies with ATP and MgATP
    Beáta Flachner
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, P O Box 7, H 1518 Budapest, Hungary
    Biochemistry 43:3436-49. 2004
    The complexes of pig muscle 3-phosphoglycerate kinase with the substrate MgATP and with the nonsubstrate Mg(2+)-free ATP have been characterized by binding, kinetic, and crystallographic studies...
  33. pmc Phosphoglycerate kinase 1 a promoting enzyme for peritoneal dissemination in gastric cancer
    Derek Zieker
    Department of General Visceral, Transplant Surgery Comprehensive Cancer Center, University of Tuebingen, D 72076 Tuebingen, Germany
    Int J Cancer 126:1513-20. 2010
    ..Previously, we showed elevated mRNA levels of phosphoglycerate kinase 1 (PGK1), an adenosine triphosphate-generating enzyme in the glycolytic pathway, the chemokine receptor 4 ..
  34. ncbi The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli
    C E Jones
    Department of Biochemistry and Molecular Biology, University College, London, England
    Eur J Biochem 233:800-8. 1995
    The overlapping genes encoding phosphoglycerate kinase (PGK) and glyceraldehyde-3-phosphate dehydrogenase (GraP-DH) from the hyperthermophilic archaeon Sulfolobus solfataricus have been cloned and sequenced...
  35. ncbi Functionally compensating coevolving positions are neither homoplasic nor conserved in clades
    Gregory B Gloor
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
    Mol Biol Evol 27:1181-91. 2010
    We demonstrated that a pair of positions in phosphoglycerate kinase that score highly by three nonparametric covariation measures are important for function even though the positions can be occupied by aliphatic, aromatic, or charged ..
  36. ncbi Phosphorylation of pyrimidine deoxynucleoside analog diphosphates: selective phosphorylation of L-nucleoside analog diphosphates by 3-phosphoglycerate kinase
    Preethi Krishnan
    Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 277:5453-9. 2002
    ..Therefore, roles of creatine kinase, 3-phosphoglycerate kinase, and pyruvate kinase were evaluated using preparations from commercial sources and human HepG2 cells...
  37. ncbi Broad specificity of human phosphoglycerate kinase for antiviral nucleoside analogs
    Sarah Gallois-Montbrun
    Unité de Régulation Enzymatique des Activités Cellulaires, CNRS URA 2185, Institut Pasteur, 75724 Paris Cedex 15, France
    Biochem Pharmacol 68:1749-56. 2004
    ..Human phosphoglycerate kinase (hPGK) was recently reported to participate in the last step of phosphorylation of cytidine L-nucleotide ..
  38. ncbi Interaction of human 3-phosphoglycerate kinase with L-ADP, the mirror image of D-ADP
    Andrea Varga
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Karolina Str 29, P O Box 7, H 1518 Budapest, Hungary
    Biochem Biophys Res Commun 366:994-1000. 2008
    ..be phosphorylated to pharmacologically active triphosphate forms, the last step seems to involve human 3-phosphoglycerate kinase (hPGK)...
  39. ncbi Substrate-assisted movement of the catalytic Lys 215 during domain closure: site-directed mutagenesis studies of human 3-phosphoglycerate kinase
    Beáta Flachner
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    Biochemistry 44:16853-65. 2005
    3-Phosphoglycerate kinase (PGK) is a two-domain hinge-bending enzyme...
  40. ncbi Crystal structure of Thermus caldophilus phosphoglycerate kinase in the open conformation
    Jun Hyuck Lee
    Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500 712, Republic of Korea
    Biochem Biophys Res Commun 350:1044-9. 2006
    b>Phosphoglycerate kinase (PGK) is a key glycolytic enzyme that catalyzes the reversible transfer of a phosphate from 1,3-bisphosphoglycerate to ADP to form 3-phosphoglycerate and ATP in the presence of magnesium...
  41. ncbi Role of side-chains in the operation of the main molecular hinge of 3-phosphoglycerate kinase
    Judit Szabo
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    FEBS Lett 582:1335-40. 2008
    ..single mutants (F165A, E192A, F196A, S392A, T393A) at and near the main hinge (beta-strand L) of human 3-phosphoglycerate kinase (hPGK) exhibit variously reduced enzyme activity, indicating the cumulative effects of these residues in ..
  42. ncbi Differences in the transient kinetics of the binding of D-ADP and its mirror image L-ADP to human 3-phosphoglycerate kinase revealed by the presence of 3-phosphoglycerate
    Claire Gondeau
    Centre d tudes d agents Pathogènes et Biotechnologies pour la Santé CPBS, UMR 5236, CNRS Université Montpellier 1 Université Montpellier 2, Montpellier Cedex 2, France
    Biochemistry 47:3462-73. 2008
    ..The last step of the cascade may be catalyzed by 3-phosphoglycerate kinase (PGK), an enzyme that has low specificity for nucleoside diphosphate (NDP): NDP + 1,3-bisphosphoglycerate ..
  43. ncbi Thermodynamic analysis of the nondenaturational conformational change of baker's yeast phosphoglycerate kinase at 24 degrees C
    Opral Ijeoma
    Department of Chemistry and Physics, Texas Woman s University, Bell Avenue, Denton, TX 76204, USA
    Arch Biochem Biophys 478:206-11. 2008
    A plot of the Gibbs free energy of unfolding vs. temperature is calculated for baker's yeast phosphoglycerate kinase in 150 mM sodium phosphate (pH=7...
  44. ncbi A 1.8 A resolution structure of pig muscle 3-phosphoglycerate kinase with bound MgADP and 3-phosphoglycerate in open conformation: new insight into the role of the nucleotide in domain closure
    A N Szilágyi
    Institute of Enzymology Biological Research Center, Hungarian Academy of Sciences, Budapest, H 1518, Hungary
    J Mol Biol 306:499-511. 2001
    3-phosphoglycerate kinase (PGK) is a typical kinase with two structural domains. The domains each bind one of the two substrates, 3-phosphoglycerate (3-PG) and MgATP...
  45. ncbi Crystal structures of substrates and products bound to the phosphoglycerate kinase active site reveal the catalytic mechanism
    B E Bernstein
    Department of Biochemistry, University of Washington, Box 357742, Seattle, Washington 98195, USA
    Biochemistry 37:4429-36. 1998
    b>Phosphoglycerate kinase (PGK) catalyzes the reversible phosphoryl transfer between 1,3-bisphosphoglycerate and ADP to form 3-phosphoglycerate and ATP in the presence of magnesium...
  46. pmc The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex
    J S Anderson
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA
    EMBO J 17:1497-506. 1998
    ..These observations argue that efficient mRNA turnover is required for viability and that we have identified the two major pathways of mRNA decay in yeast...
  47. ncbi Closed structure of phosphoglycerate kinase from Thermotoga maritima reveals the catalytic mechanism and determinants of thermal stability
    G Auerbach
    Max Planck Institut fur Biochemie, Abt Strukturforschung, 82152, Martinsried, Germany
    Structure 5:1475-83. 1997
    b>Phosphoglycerate kinase (PGK) is essential in most living cells both for ATP generation in the glycolytic pathway of aerobes and for fermentation in anaerobes. In addition, in many plants the enzyme is involved in carbon fixation...
  48. ncbi Interaction of human 3-phosphoglycerate kinase with its two substrates: is substrate antagonism a kinetic advantage?
    Perrine Lallemand
    Centre d études d agents Pathogènes et Biotechnologies pour le Santé, UMR 5236, CNRS Universités Montpellier 1 and 2, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Mol Biol 409:742-57. 2011
    ..3-Phosphoglycerate kinase (PGK) catalyzes phosphotransfer from 1,3-bisphosphoglycerate (bPG) to ADP to give 3-phosphoglycerate (PG) ..
  49. pmc Crystallization and preliminary X-ray diffraction analysis of phosphoglycerate kinase from Streptococcus pneumoniae
    Noelia Bernardo-García
    Department of Crystallography and Structural Biology, Instituto de Quimica Fisica Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1285-9. 2011
    b>Phosphoglycerate kinase (PGK) is a widespread two-domain enzyme that plays a critical role in the glycolytic pathway...
  50. ncbi Structural and energetic basis of protein kinetic destabilization in human phosphoglycerate kinase 1 deficiency
    Angel L Pey
    Department of Physical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
    Biochemistry 52:1160-70. 2013
    Protein kinetic destabilization is a common feature of many human genetic diseases. Human phosphoglycerate kinase 1 (PGK1) deficiency is a rare genetic disease caused by mutations in the PGK1 protein, which often shows reduced kinetic ..
  51. pmc Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants
    Shun Fang Cheng
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan
    Plant Physiol 163:1598-608. 2013
    ..Previously, we identified that the chloroplast phosphoglycerate kinase (chl-PGK) from Nicotiana benthamiana is one of the viral RNA binding proteins involved in the BaMV ..
  52. ncbi Microarray analysis of rat immune responses to liver fluke infection following vaccination with Fasciola hepatica phosphoglycerate kinase
    Agnieszka Wesołowska
    Department of Molecular Biology, Laboratory of Molecular Parasitology, W Stefanski Institute of Parasitology, Polish Academy of Sciences, 51 55 Twarda, 00 818 Warsaw, Poland
    Exp Parasitol 134:33-8. 2013
    ..hepatica following immunization with F. hepatica phosphoglycerate kinase - a novel vaccine antigen...
  53. ncbi Redox control of the activity of phosphoglycerate kinase in Synechocystis sp. PCC6803
    Yu Tsukamoto
    Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259 R1 8, Midori ku, Yokohama, 226 8503 Japan
    Plant Cell Physiol 54:484-91. 2013
    b>Phosphoglycerate kinase catalyzes the phosphorylation of 3-phosphoglycerate to 1,3-diphosphoglycerate using ATP within the Calvin-Benson cycle. Recent proteomic analysis of thioredoxin targets in Synechocystis sp...
  54. pmc Molecular insights on pathogenic effects of mutations causing phosphoglycerate kinase deficiency
    Laurent R Chiarelli
    Dipartimento di Biologia e Biotecnologie L Spallanzani, Universita degli Studi di Pavia, Pavia, Italy
    PLoS ONE 7:e32065. 2012
    b>Phosphoglycerate kinase (PGK) catalyzes an important ATP-generating step in glycolysis...
  55. ncbi Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells
    Kasturi Mukherjee
    Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata 700 032, India
    Indian J Biochem Biophys 39:332-41. 2002
    3-Phosphoglycerate kinase (3-PGK) has been purified to apparent homogeneity from Ehrlich ascites carcinoma (EAC) cells by (NH4)2SO4 precipitation, gel filtration and ion-exchange chromatography...
  56. ncbi Dimeric 3-phosphoglycerate kinases from hyperthermophilic Archaea. Cloning, sequencing and expression of the 3-phosphoglycerate kinase gene of Pyrococcus woesei in Escherichia coli and characterization of the protein. Structural and functional comparison
    D Hess
    FB 9 Mikrobiologie, Universitat Essen, Germany
    Eur J Biochem 233:227-37. 1995
    The gene coding for the 3-phosphoglycerate kinase (EC 2.7.2.3) of Pyrococcus woesei was cloned and sequenced. The gene sequence comprises 1230 bp coding for a polypeptide with the theoretical M(r) of 46,195...
  57. pmc Identification of the actin and plasminogen binding regions of group B streptococcal phosphoglycerate kinase
    Tyler J Boone
    Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada
    J Biol Chem 287:29035-44. 2012
    b>Phosphoglycerate kinase (PGK), present on the surface of group B streptococcus (GBS), has previously been demonstrated to bind the host proteins actin and plasminogen...
  58. pmc Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice
    Polina V Danshina
    Laboratories for Reproductive Biology, Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7090, USA
    Biol Reprod 82:136-45. 2010
    b>Phosphoglycerate kinase 2 (PGK2), an isozyme that catalyzes the first ATP-generating step in the glycolytic pathway, is encoded by an autosomal retrogene that is expressed only during spermatogenesis...
  59. ncbi Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgA
    Roberto Calcedo
    Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country, Spain
    Rev Iberoam Micol 29:172-4. 2012
    ..Candida albicans is an opportunistic dimorphic fungus commonly present in the human oral cavity that causes infections in immunocompromised patients. The antigen variability, influenced by growth conditions, is a pathogenicity factor...
  60. ncbi Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster
    L Roselli-Rehfuss
    Department of Biology, Syracuse University, New York 13244
    Mol Gen Genet 235:213-20. 1992
    The gene that encodes phosphoglycerate kinase in Drosophila melanogaster (Pgk) has been isolated and characterized. There is a single copy of Pgk in the Drosophila genome located at cytogenetic position 23A1-2. Transcripts of Pgk are 1...
  61. ncbi An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase
    P Branny
    Laboratoire d Enzymologie et de Biochimie Structurales du CNRS, Gif sur Yvette, France
    Microbiology 144:905-14. 1998
    ..gap, pgk and tpi encoding three glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI), respectively, have been cloned and sequenced from ..
  62. pmc Comparing the folding and misfolding energy landscapes of phosphoglycerate kinase
    Gergely Agócs
    Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
    Biophys J 102:2828-34. 2012
    ..Using yeast phosphoglycerate kinase as model, here we identify the features of the energy landscape that decide the fate of the protein: ..
  63. pmc A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells
    Mingang Xu
    Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, Philadelphia, PA 6080, USA
    Nucleic Acids Res 36:7157-67. 2008
    b>Phosphoglycerate kinase 2 (PGK2) is a germ cell-specific protein whose mRNA is translationally regulated in the mammalian testis...
  64. ncbi Functional identity of a primer recognition protein as phosphoglycerate kinase
    H K Jindal
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68105
    J Biol Chem 265:6540-3. 1990
    ..search against a protein sequence data base, we have identified PRP 2 to be the glycolytic enzyme, phosphoglycerate kinase (PGK). The activities of PRP and PGK increase coordinately in the PRP purification procedure...
  65. ncbi PGK1, the gene encoding the glycolitic enzyme phosphoglycerate kinase, acts as a multicopy suppressor of apoptotic phenotypes in S. cerevisiae
    Cristina Mazzoni
    Pasteur Institute Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    Yeast 26:31-7. 2009
    ..by overexpressing yeast genes allowed the isolation of PGK1, the gene encoding the glycolytic enzyme phosphoglycerate kinase. This gene restored normal ageing, oxygen peroxide resistance and nuclear integrity in the mutant...
  66. ncbi Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex
    E M Eicher
    Mol Gen Genet 158:225-8. 1978
    The mouse autosomal locus that determines the form of phosphoglycerate kinase found only in testes is shown here to be closely linked to but not included within the major histocompatibility complex on chromosome 17...
  67. ncbi Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycin
    C M Grant
    Biological Laboratory, University of Kent, Canterbury, UK
    Curr Genet 26:95-9. 1994
    ..However, paromomycin-induced mistranslation of a heterologous mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen...
  68. ncbi Molecular defect of a phosphoglycerate kinase variant (PGK-Matsue) associated with hemolytic anemia: Leu----Pro substitution caused by T/A----C/G transition in exon 3
    M Maeda
    Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, CA 91010
    Blood 77:1348-52. 1991
    We have identified the mutation in a phosphoglycerate kinase variant (PGK-Matsue) associated with severe enzyme deficiency, congenital nonspherocytic hemolytic anemia, and mental disorders...
  69. ncbi Molecular abnormalities of a phosphoglycerate kinase variant generated by spontaneous mutation
    M Maeda
    Department of Biochemical Genetics, Beckman Research Institute, City of Hope, Duarte, CA 91010
    Blood 79:2759-62. 1992
    A new case of X chromosome-linked phosphoglycerate kinase (PGK) abnormality is described. The male proband was mentally retarded, had behavior disorders, and displayed episodes of hemolytic anemia...
  70. ncbi Enzymatic activity of immobilized yeast phosphoglycerate kinase
    Cedric Hurth
    Centre de Physique Moleculaire Optique et Hertzienne, 33600 Pessac, France
    Biosens Bioelectron 22:2449-55. 2007
    This work reports the first evidence that recombinant yeast phosphoglycerate kinase (PGK) is still significantly active when immobilized on glass and muscovite mica...
  71. ncbi The identification of a recurrent phosphoglycerate kinase mutation associated with chronic haemolytic anaemia and neurological dysfunction in a family from USA
    Jonathan M Flanagan
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, MEM 215, 10550 Torrey Pines, La Jolla, CA, USA
    Br J Haematol 134:233-7. 2006
    b>Phosphoglycerate kinase (PGK) deficiency is a rare X-linked disease that is characterised by mild to severe haemolytic anaemia, rhabdomyolysis, and variable defects in the central nervous system...
  72. ncbi Altered expression of PGK1 in a family with phosphoglycerate kinase deficiency
    Eva K Svaasand
    Department of Pathology and Medical Genetics, St Olav Hospital, Trondheim, Norway
    Muscle Nerve 36:679-84. 2007
    The X-linked recessive disease phosphoglycerate kinase (PGK) deficiency is caused by altered expression of the PGK1 enzyme, which causes muscle stiffness, hemolytic anemia, and mental retardation...
  73. ncbi Gene expression and clonality analysis of the androgen receptor and phosphoglycerate kinase genes in polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma
    En Hua Wang
    Department of Pathology, College of Basic Medical Sciences, China Medical University, and First Affiliated Hospital of China Medical University, Shenyang, China
    Mod Pathol 20:1208-15. 2007
    ..with the methylation-sensitive enzymes HhaI or HpaII, followed by nested PCR of the androgen receptor and phosphoglycerate kinase genes. Samples with polymorphisms were identified and a clonality analysis was performed...
  74. ncbi A single amino acid substitution (157 Gly----Val) in a phosphoglycerate kinase variant (PGK Shizuoka) associated with chronic hemolysis and myoglobinuria
    H Fujii
    Department of Blood Transfusion Medicine, Tokyo Women s Medical College, Japan
    Blood 79:1582-5. 1992
    We have determined a single amino acid substitution in a new phosphoglycerate kinase (PGK) variant, PGK Shizuoka, associated with chronic hemolysis and myoglobinuria...
  75. ncbi Phosphoglycerate kinase deficiency in two brothers with McArdle-like clinical symptoms
    J Aasly
    Department of Neurology, University Hospital, Trondheim, Norway
    Eur J Neurol 7:111-3. 2000
    b>Phosphoglycerate kinase (PGK) catalyses the transfer of the acylphosphate group of 1,3-diphosphoglycerate to ADP with formation of 3-phosphoglycerate and ATP in the terminal stage of the glycolytic pathway...
  76. ncbi Intronic mutation in the PGK1 gene may cause recurrent myoglobinuria by aberrant splicing
    Kentaro Shirakawa
    First Department of Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Neurology 66:925-7. 2006
    A 33-year-old man with mental retardation and recurrent myoglobinuria demonstrated a deficiency in the phosphoglycerate kinase 1 (PGK1) activity of his muscles and erythrocytes...
  77. ncbi Insight into the mechanism of domain movements and their role in enzyme function: example of 3-phosphoglycerate kinase
    M Vas
    Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, H 1518 Budapest, P O Box 7, Hungary
    Curr Protein Pept Sci 11:118-47. 2010
    ..In this review we summarize comprehensive studies with a typical hinge-bending two-domain enzyme, 3-phosphoglycerate kinase. A possible mechanism is proposed by which the two substrates that bind to different domains trigger the ..
  78. ncbi Identification of new mutations in two phosphoglycerate kinase (PGK) variants expressing different clinical syndromes: PGK Créteil and PGK Amiens
    M Cohen-Solal
    Unité INSERM U91, Hopital Henri Mondor, Creteil, France
    Blood 84:898-903. 1994
    b>Phosphoglycerate kinase (PGK) deficiency is generally associated with chronic hemolytic anemia, although it can be accompanied by either mental retardation or muscular disease...
  79. ncbi Retarded and aberrant splicings caused by single exon mutation in a phosphoglycerate kinase variant
    T Ookawara
    Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Arch Biochem Biophys 327:35-40. 1996
    The molecular abnormality of a phosphoglycerate kinase variant which was associated with severe tissue enzyme deficiency and episodes of muscle contractions and myoglobinuria was examined...
  80. ncbi Molecular abnormality of a phosphoglycerate kinase variant (PGK-Alabama)
    A Yoshida
    Department of Biochemical Genetics, Beckman Institute of the City of Hope, Duarte, California, USA
    Blood Cells Mol Dis 21:179-81. 1995
    The molecular abnormality of a phosphoglycerate kinase variant associated with severe red cell enzyme deficiency ( about 4% of normal) and episodes of hemolysis with jaundice was examined...
  81. ncbi Two proliferation-related proteins, TYMS and PGK1, could be new cytotoxic T lymphocyte-directed tumor-associated antigens of HLA-A2+ colon cancer
    Shigeki Shichijo
    Department of Immunology, Kurume University School of Medicine, Research Center of Innovative Cancer Therapy of the 21st Century COE Program for Medical Science, Kurume University, Kurume, Fukuoka, Japan
    Clin Cancer Res 10:5828-36. 2004
    ..The purpose of this work was to provide a scientific basis for specific immunotherapy of colon cancer...
  82. ncbi Substrate binding modifies the hinge bending characteristics of human 3-phosphoglycerate kinase: a molecular dynamics study
    Zoltan Palmai
    Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Hungary
    Proteins 77:319-29. 2009
    ..At the same time, ligand binding results in an enhanced correlation of internal domain motions...
  83. ncbi A single amino acid substitution (Asp leads to Asn) in a phosphoglycerate kinase variant (PGK München) associated with enzyme deficiency
    H Fujii
    J Biol Chem 255:6421-3. 1980
    The structural abnormality of the phosphoglycerate kinase variant, PGK München, associated with red cell enzyme deficiency and heat instability, was elucidated by a microscale peptide-mapping method...
  84. ncbi Enzymatic and metabolic characterization of the phosphoglycerate kinase deficiency associated with chronic hemolytic anemia caused by the PGK-Barcelona mutation
    María José Ramírez-Bajo
    Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Spain
    Blood Cells Mol Dis 46:206-11. 2011
    Recently, we reported a new mutation of phosphoglycerate kinase (PGK), called PGK-Barcelona, which causes chronic hemolytic anemia associated with progressive neurological impairment...
  85. ncbi A phosphoglycerate kinase mutant (PGK Herlev; D285V) in a Danish patient with isolated chronic hemolytic anemia: mechanism of mutation and structure-function relationships
    C Valentin
    Unité INSERM U 474, Hopital Henri Mondor, Creteil, France
    Hum Mutat 12:280-7. 1998
    b>Phosphoglycerate kinase (PGK) is a X-linked enzyme that plays a key role in the glycolytic pathway. Twelve different variants have already been reported...
  86. pmc Molecular abnormality of phosphoglycerate kinase-Uppsala associated with chronic nonspherocytic hemolytic anemia
    H Fujii
    Proc Natl Acad Sci U S A 77:5461-5. 1980
    Inherited deficiency of phosphoglycerate kinase (PGK; ATP:3-phosphoglycerate 1-phosphotransferase, EC 2.7.2.3) is associated with chronic nonspherocytic hemolytic anemia and mental disorders in man...
  87. pmc Use of cultured lymphoblastoid cells for the study of abnormal enzymes: molecular abnormality of a phosphoglycerate kinase variant associated with hemolytic anemia
    H Fujii
    Proc Natl Acad Sci U S A 78:2587-90. 1981
    A phosphoglycerate kinase (PGKase: ATP:3-phosphoglycerate 1-phosphotransferase, EC 2.7.2.3; X chromosome-linked) variant, PGKase-Tokyo, is associated with enzyme deficiency, nonspherocytic hemolytic anemia, and neurological disturbances...
  88. ncbi Phosphoglycerate kinase 1-overexpressing lung cancer cells reduce cyclooxygenase 2 expression and promote anti-tumor immunity in vivo
    Shye Jye Tang
    Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, ROC
    Int J Cancer 123:2840-8. 2008
    In addition to the known function in the glycolytic pathway, phosphoglycerate kinase 1 (PGK-1) promotes reduction of plasmin disulfide bonds leading to angiostatin formation and inhibition of tumor angiogenesis...
  89. ncbi Variation for X chromosome expression in mice detected by electrophoresis of phosphoglycerate kinase
    J D West
    Genet Res 32:91-102. 1978
  90. ncbi Native and unfolded states of phosphoglycerate kinase studied by single-molecule FRET
    Tobias Rosenkranz
    Research Centre Julich, ISB 2 Molecular Biophysics, 52425 Julich, Germany
    Chemphyschem 12:704-10. 2011
    Single-molecule Förster resonance energy transfer (FRET) measurements with phosphoglycerate kinase from yeast were performed at different concentrations of guanidine hydrochloride...
  91. ncbi A novel missense mutation (1060G --> C) in the phosphoglycerate kinase gene in a Japanese boy with chronic haemolytic anaemia, developmental delay and rhabdomyolysis
    Akira Morimoto
    Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan
    Br J Haematol 122:1009-13. 2003
    We report the case of a 3-year-old Japanese boy with phosphoglycerate kinase 1 (PGK1) deficiency (Online Mendelian Inheritance in Man entry 311800)...
  92. ncbi A sandwich ELISA for phosphoglycerate kinase
    Weilin Zhao
    Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York 11203, USA
    J Immunoassay Immunochem 29:220-33. 2008
    b>Phosphoglycerate kinase (PGK1) is a key enzyme in glycolysis that can also be released from certain cells...
  93. ncbi Nucleotide-binding domain of phosphoglycerate kinase 1 reduces tumor growth by suppressing COX-2 expression
    Ming Yi Ho
    Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Department of Education and Research, Taipei City Hospital, Taipei, Taiwan
    Cancer Sci 101:2411-6. 2010
    b>Phosphoglycerate kinase 1 (PGK-1) is a multifunctional protein that is involved in the glycolytic pathway and the generation of the angiogenesis inhibitor angiostatin...
  94. ncbi Blocking Fasciola hepatica's energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinase
    Slawomir Jaros
    Department of Molecular Biology, Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland
    Vet Parasitol 172:229-37. 2010
    ..This paper is focused on first vaccination trials based on a new vaccine candidate antigen, F. hepatica phosphoglycerate kinase (FhPGK) performed on a rat model...
  95. ncbi Immunoelectron microscopic analysis of the intracellular distribution of primer recognition proteins, annexin 2 and phosphoglycerate kinase, in normal and transformed cells
    K D Kumble
    Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198 4525
    J Cell Sci 99:751-8. 1991
    ..We have previously reported that the PRP consist of a complex of two proteins identified as 3-phosphoglycerate kinase (PGK) and the protein-tyrosine kinase substrate, annexin 2 monomer...
  96. ncbi Denatured states of yeast phosphoglycerate kinase
    G Damaschun
    Institut für Biologie der Humboldt Universität zu Berlin, Germany
    Biochemistry (Mosc) 63:259-75. 1998
    ..Acid-denatured, cold-denatured, and 6 M guanidine hydrochloride (GuHCl) denatured yeast phosphoglycerate kinase (PGK) are ensembles of flexible unfolded molecules with rapidly interconverting structures of the ..
  97. ncbi Processing of a phosphoglycerate kinase reporter mRNA in Trypanosoma brucei is not coupled to transcription by RNA polymerase II
    Mhairi Stewart
    Zentrum für Molekularbiologie der Universität Heidelberg, ZMBH DKFZ Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Mol Biochem Parasitol 172:99-106. 2010
    ..There was therefore no evidence for recruitment of the 3'-splicing apparatus by the RNA polymerase. Abundant transcripts transcribed from the rRNA locus by T7 RNA polymerase were somewhat less efficiently processed...
  98. ncbi Structural tightening and interdomain communication in the catalytic cycle of phosphoglycerate kinase
    James P Marston
    Department of Molecular Biology and Biotechnology, Firth Court, The University of Sheffield, Western Bank, Sheffield, UK
    J Mol Biol 396:345-60. 2010
    Changes in amide-NH chemical shift and hydrogen exchange rates as phosphoglycerate kinase progresses through its catalytic cycle have been measured to assess whether they correlate with changes in hydrogen bonding within the protein...
  99. pmc Entry of Spiroplasma citri into Circulifer haematoceps cells involves interaction between spiroplasma phosphoglycerate kinase and leafhopper actin
    Fabien Labroussaa
    UMR 1090 Génomique Diversité Pouvoir Pathogène, INRA, Universite Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP 81, F 33883 Villenave d Ornon, France
    Appl Environ Microbiol 76:1879-86. 2010
    ..actin-binding protein of 44 kDa was isolated by affinity chromatography and identified by LC-MS/MS as phosphoglycerate kinase (PGK)...
  100. pmc The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes
    Antonio M Estevez
    Instituto de Parasitologia y Biomedicina Lopez Neyra, CSIC Avda del Conocimiento s n, Armilla, 18100 Granada, Spain
    Nucleic Acids Res 36:4573-86. 2008
    ..These observations raise the possibility that TbDRBD3-mRNA complexes act as a post-transcriptional operon, and provide a framework to interpret how trypanosomes regulate gene expression in the absence of transcriptional control...
  101. pmc Foamy-virus-mediated gene transfer to canine repopulating cells
    Hans Peter Kiem
    Clinical Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N D1 100, PO Box 19024, Seattle, WA 98109 1024, USA
    Blood 109:65-70. 2007
    ..These data suggest that FV vectors should be useful for stem-cell gene therapy, particularly for applications in which short transduction protocols are critical...

Research Grants34

  1. Glycolysis and Glutamate Storage into Synaptic Vesicles
    Tetsufumi Ueda; Fiscal Year: 2010
    ..We have recently provided evidence that glyceraldehyde phosphate dehydrogenase and 3-phosphoglycerate kinase, glycolytic enzymes responsible for production of ATP, are associated with synaptic vesicles and that ..
  2. Inhibitors of Trypanosomatid Phosphoglycerate Mutase and Phosphoglycerate Kinase
    MALCOLM DOUGLAS WALKINSHAW; Fiscal Year: 2012
    ..of this project is to discover and optimize selective inhibitors of phosphoglycerate mutase (PGAM) and phosphoglycerate kinase (PGK) of trypanosomatid parasites (Trypanosoma brucei, T. cruzi and Leishmania species)...
  3. Lentiviral Vectors for the Treatment of Fanconi Anemia
    DAVID W CLAPP; Fiscal Year: 2010
    ..1: Generate recombinant lentiviral constructs that encode the expression of the FANCA cDNA by the human phosphoglycerate kinase promoter or the elongation factor 1a promoter and test functional expression of the transgenes in murine ..
  4. NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDS
    Yung Chi Cheng; Fiscal Year: 2010
    ..2. To examine the role of Hypoxia: a. in regulating phosphoglycerate kinase (PGK), which is responsible for the phosphorylation of L- OddCDP to L-OddCTP. b...
  5. CONTROL OF TRANSCRIPTION OF ISOLATED EUCARYOTIC GENES
    Michael Holland; Fiscal Year: 1980
    ..DNAs containing genomic DNA sequences which code for yeast glyceraldehyde 3-phosphate dehydrogenase, phosphoglycerate kinase, enolase and phosphoglycerate mutase have been isolated in this laboratory and will be fundamental to the ..
  6. X-CHROMOSOME INACTIVATION IN OPOSSUM DEVELOPMENT
    PAUL SAMOLLOW; Fiscal Year: 1991
    ..relative levels of expression of the Gpd and Pgk-A genes (encode glucose-6-phosphate dehydrogenase and phosphoglycerate kinase-A, respectively on the maternal and paternal X chromosomes in tissues and organs of newborn pouch young, ..
  7. MOLECULAR PATHOPHYSIOLOGY OF THYROID CELL GROWTH
    James Fagin; Fiscal Year: 1993
    ..advantage of polymorphisms in the X-chromosome genes hypoxanthine phosphorybosiltransferase (HPRT) and phosphoglycerate kinase (PGK)...
  8. CLONALITY IN ACUTE MYELOID LEUKEMIA AND MYELODYSPLASIA
    Donald Kufe; Fiscal Year: 1992
    ..via noting allelic loss of a restriction fragment length polymorphism (RFLP) associated with the X-linked phosphoglycerate kinase (PGK) gene...
  9. GENE REGULATION IN SPERMATOGENESIS
    John McCarrey; Fiscal Year: 1992
    The mammalian phosphoglycerate kinase (PGK) gene family remains unparalleled in the number of different facets of gene regulation that impinge upon the expression of these genes in spermatogenesis...
  10. TIME-RESOLVED FLUORESCENCE STUDIES OF PROTEIN FOLDING
    Joseph Beechem; Fiscal Year: 1999
    ..Experimental efforts are concentrated on the examination of the folding of yeast phosphoglycerate kinase (PGK) and E. coli alpha- subunit of tryptophan synthase (alpha-TS...
  11. MEMBRANE TRANSPORT PROCESSES IN RED BLOOD CELLS
    Joseph Hoffman; Fiscal Year: 1991
    ..situations, these parameters will also be followed together with the possible involvement of membrane phosphoglycerate kinase and Na,K-ATPase defining a membrane pool of ATP...
  12. ADAPTATION TO ENVIRONMENTAL STRESS--MECHANISTIC ANALYSES
    James Whitlock; Fiscal Year: 2000
    ..The experiments involve analyses of the response of the phosphoglycerate kinase 1 and aldolase A genes to hypoxia, genetic analyses of wild-type and mutant forms of HIF1a, and ..
  13. DOMAIN INTERACTION IN GLOBULAR AND TRANSMEMBRANE PROTEIN
    JOHN BRANDTS; Fiscal Year: 1993
    ..A well-defined, two-domain, soluble enzyme from yeast- 3-phosphoglycerate kinase (PG32, and 2.) A membrane- spanning protein, the aspartate receptor (AR) of S...
  14. NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDS
    Yung Chi Cheng; Fiscal Year: 2007
    ..2. To examine the role of Hypoxia: a. in regulating phosphoglycerate kinase (PGK), which is responsible for the phosphorylation of L- OddCDP to L-OddCTP. b...
  15. AGE RELATED CONFORMATIONAL MODIFICATIONS OF PROTEIN
    Ari Gafni; Fiscal Year: 2003
    ..We will focus on two proteins, the glycolytic enzyme phosphoglycerate kinase (PGK) and the fibrinolysis-associated protein plasminogen activator inhibitor-1 (PAI-1)...
  16. Regulation of Gene Expression by DNA Methylation
    John McCarrey; Fiscal Year: 2002
    ..This is the case for the autosomal phosphoglycerate kinase (Pgk2), which is expressed specifically in spermatogenic cells...
  17. SUBMILLISECOND LASER T JUMP INDUCED PROTEIN FOLDING
    Martin Gruebele; Fiscal Year: 2001
    ..Our initially proposed systems include apomyoglobin, cytochrome c, ubiquitin, and phosphoglycerate kinase, all of which have known sub-ms folding phases...
  18. IMMUNOGENETIC STUDIES OF A SPERM ENZYME PGK-B
    JOHN VANDEBERG; Fiscal Year: 1980
    The overall objectives are to characterize the sperm enzyme phosphoglycerate kinase B (PGK-B) immunogenetically and immunochemically and to determine its role in reproduction...
  19. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2004
    ..Our preliminary data support the hypothesis and show that phosphoglycerate kinase (PGK) and other newly recognized uPAR and uPA mRNA binding protein-mRNA interactions control uPAR and uPA ..
  20. Regulation of Lung Epithelial Fibrinolysis by Urokinase
    Sreerama Shetty; Fiscal Year: 2005
    ..This information could hasten the development of new, mechanism-based interventions for lung disorders, including acute lung injury or lung neoplasia. ..
  21. Single Cell Analysis of Cross Talk Among Kinase Pathways
    Stefan Kaluz; Fiscal Year: 2008
    ..These studies will provide additional information relating to our understanding of the consequencesof activationof kinase signaling pathwayswith respect to malignant transformation, aggressive tumor growth and survival. ..
  22. Cord Blood Expansion and Transplantation in a Nonhuman Primate Model
    Hans Peter Kiem; Fiscal Year: 2009
    ....
  23. Gene therapy for Fanconi anemia
    Hans Peter Kiem; Fiscal Year: 2009
    ..Our ultimate goal is to improve or cure the hematopoietic symptoms in FA patients by lentivirus-mediated HSC gene transfer, and develop methodologies that would be applicable to other genetic disorders and hematological diseases. ..
  24. IL-12-Faciliated Hematopoietic Recovery Following Myeloablative Therapy
    Dan Douer; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  25. CLINICAL RESEARCH CENTER FOR NEUROMUSCULAR DISEASE
    Salvatore DiMauro; Fiscal Year: 2007
    ....
  26. MGMT-Mediated in vivo Selection in a Large Animal Model
    Hans Peter Kiem; Fiscal Year: 2006
    ..These experiments will provide a platform for studies using gene-modified allogeneic stem cells to improve nonmyeloablative transplant strategies. ..
  27. Testicular Torsion: Mechanisms and Treatment
    Terry Turner; Fiscal Year: 2005
    ..It is likely that these investigations will be relevant to other forms of oxidative stress inducing testicular dysfunction. ..
  28. Arsenic Trioxide and Vitamin C in AML
    Dan Douer; Fiscal Year: 2005
    ..In summary, this combination may have higher benefit/risk ratio in non-APL AML patients resulting in an overall better outcome with less toxicity than conventional chemotherapy. ..
  29. A Mouse Model of Gaucher Disease
    Ernest Beutler; Fiscal Year: 2005
    ..In particular, electron microscopy and light microscopy will be used to attempt to demonstrate the development of Gaucher cells in the chimeric mice. ..
  30. Transcriptional Regulation of a Surfactant Enzyme
    RAMA MALLAMPALLI; Fiscal Year: 2003
    ..Ultimately, the results from these studies could lead to therapeutic strategies directed at modulating expression of this key surfactant enzyme at the molecular level. ..
  31. RETROVIRAL TRANSFER OF ANKYRIN FOR SPHEROCYTOSIS
    Pamela Becker; Fiscal Year: 2002
    ..abstract_text> ..
  32. FORMATION AND SURVIVAL OF RED BLOOD CELLS
    Ernest Beutler; Fiscal Year: 2001
    ..Should these point mutations fail to alter gene transcription, a search will be made for other potential mutations in linkage disequilibrium with the known -5,-8 and -24 polymorphisms, by sequencing upstream through the promotor. ..
  33. Hematopoietic Potential of Muscle-Derived Cells
    Hans Peter Kiem; Fiscal Year: 2004
    ..abstract_text> ..