Genomes and Genes
Summary: Allosteric enzymes that regulate glycolysis and gluconeogenesis. These enzymes catalyze phosphorylation of fructose-6-phosphate to either fructose-1,6-bisphosphate (PHOSPHOFRUCTOKINASE-1 reaction), or to fructose-2,6-bisphosphate (PHOSPHOFRUCTOKINASE-2 reaction).
Publications180 found, 100 shown here
- Characterization of glycolytic enzyme interactions with murine erythrocyte membranes in wild-type and membrane protein knockout miceM Estela Campanella
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
Blood 112:3900-6. 2008..These data suggest that oxygenation-dependent assembly of GEs on the membrane could be a general phenomenon of mammalian erythrocytes and that stability of these interactions depends primarily on band 3...
- Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sourcesMichelle R Lovingshimer
Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
Protein Expr Purif 46:475-82. 2006..In addition to having both pfkA and pfkB deleted, RL257 contains the lacI(q) allele, which allows for inducible expression when coupled with an expression vector containing either the lac or tac promoter...
- Existence of a true phosphofructokinase in Bacillus sphaericus: cloning and sequencing of the pfk geneAlejandro F Alice
Laboratorio de Microbiologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Appl Environ Microbiol 68:6410-5. 2002..A careful sequence analysis revealed the conservation of different catalytic and regulatory residues, as well as the enzyme's phylogenetic affiliation with the family of allosteric ATP-PFK enzymes...
- On the mechanisms of glycolytic oscillations in yeastMads F Madsen
Functional Dynamics Group, Department of Chemistry, University of Copenhagen, Denmark
FEBS J 272:2648-60. 2005....
- Yeast glycolytic oscillations that are not controlled by a single oscillophore: a new definition of oscillophore strengthKarin A Reijenga
Department of Molecular Cell Physiology, CRbCS, BioCentrum Amsterdam, Faculty of Earth and Life Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
J Theor Biol 232:385-98. 2005..These results constitute proof that glycolytic oscillations in yeast are not caused by a single oscillophore and provide a new, subtle, definition for the oscillophore strength of an enzyme...
- The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinasesIain W McNae
Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh, Scotland
J Mol Biol 385:1519-33. 2009..brucei PFK compared with bacterial and mammalian PFKs give optimism for the discovery of species-specific drugs for the treatment of diseases caused by protist parasites of the trypanosomatid family...
- The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscleKatarzyna Banaszak
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
J Mol Biol 407:284-97. 2011..Crystallographic results have been used as the basis for structure-based effector design...
- Turing patterns inside cellsDamián E Strier
Service de Chimie Physique and Center for Nonlinear Phenomena and Complex Systems, Universite Libre de Bruxelles, Brussels, Belgium
PLoS ONE 2:e1053. 2007..Turing patterns inside cells could provide a check-point that combines mechanical and biochemical information to trigger events during the cell division process...
- Glycolytic oscillations and waves in an open spatial reactor: Impact of feedback regulation of phosphofructokinaseSatenik Bagyan
Institute of Experimental Physic, Group of Biophysics, Otto von Guericke University of Magdeburg, Universitatsplatz 2, D 39106 Magdeburg, Germany
Biophys Chem 116:67-76. 2005..The generation of travelling NADH-waves is observed in the diffusive layer, when feeding the yeast extract with substrates. Break-up of circular-shaped waves is repeatedly observed, resulting in the formation of rotating NADH-spirals...
- Inward rotating spiral waves in glycolysisRonny Straube
Max Planck Institute for Dynamics of Complex Technical Systems, Systems Biology Group, Sandtorstr 1, Magdeburg, Germany
Biophys J 99:L4-6. 2010..Numerical simulations with an allosteric model for the phosphofructokinase can reproduce these inward propagating waves over a wide range of parameters if the octameric structure of yeast phosphofructokinase is taken into account...
- The biochemical properties and phylogenies of phosphofructokinases from extremophilesR S Ronimus
Thermophile Research Unit, Department of Biological Sciences, University of Waikato, Hamilton, New Zealand
Extremophiles 5:357-73. 2001..the crenarchaeal pyrophosphate-dependent PFK of Thermoprotetus tenax) and plant pyrophosphate-dependent phosphofructokinases; the PFKB family, exemplified by the minor ATP-dependent PFK activity of Escherichia coli (PFK 2), but ..
- Glycolytic sequence and respiration of Debaryomyces hansenii as compared to Saccharomyces cerevisiaeN S Sánchez
Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Circuito exterior s n, Ciudad Universitaria, D F 04510, Mexico
Yeast 23:361-74. 2006..cerevisiae but not in D. hansenii. D. hansenii shows an alternative oxidase, which in our experiments did not appear to be coupled to the production of ATP...
- Ethanolic extract of Ocimum sanctum leaves partially attenuates streptozotocin-induced alterations in glycogen content and carbohydrate metabolism in ratsV Vats
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110049, India
J Ethnopharmacol 90:155-60. 2004..OS did not affect glycogen content in any tissue. Activity of HK, GK and PFK in diabetic controls was 35, 50 and 60% of the controls and OS partially corrected this alteration...
- Detachment of glycolytic enzymes from cytoskeleton of Lewis lung carcinoma and colon adenocarcinoma cells induced by clotrimazole and its correlation to cell viability and morphologyJulia Penso
Health Sciences Research Center, Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
Mol Genet Metab 76:181-8. 2002....
- Shift in metabolic fuel in acylation-stimulating protein-deficient mice following a high-fat dietChristian Roy
Centre de recherche Hopital Laval, Y 2186, Chemin Ste Foy, QC, Canada
Am J Physiol Endocrinol Metab 294:E1051-9. 2008..However, these in vivo data suggest that ASP inhibition could be a potentially viable approach in correcting muscle metabolic dysfunction in obesity...
- Significant increase of glycolytic flux in Torulopsis glabrata by inhibition of oxidative phosphorylationLiming Liu
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, China
FEMS Yeast Res 6:1117-29. 2006..9967) and the average specific pyruvate production rate (R2=0.965) were closely correlated with the intracellular ATP level, all of them being increased at a lower intracellular ATP level...
- Design, synthesis and trypanocidal activity of lead compounds based on inhibitors of parasite glycolysisMatthew W Nowicki
School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK
Bioorg Med Chem 16:5050-61. 2008..Trypanocidal activity also showed potency in the low micromolar range and confirms these inhibitors as promising candidates for the development towards the design of anti-trypanosomal drugs...
- [An experimental study of the changes of rat hepatocytic glycolysis during hypoxia]Zhengwei Ma
Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, P R China
Zhonghua Shao Shang Za Zhi 18:238-41. 2002..To investigate the effects of hypoxia on the glycolysis in cultured rat hepatocytes...
- Effect of feeding aqueous extract of Pterocarpus marsupium on glycogen content of tissues and the key enzymes of carbohydrate metabolismJagdish Kumari Grover
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India
Mol Cell Biochem 241:53-9. 2002..Activity of HK, GK and PFK in diabetic controls was 35,50 and 60% of the controls and PM completely corrected this alteration in PFK and only partly in HK and GK...
- The effects of acclimation to reversed seasonal temperatures on the swimming performance of adult brown trout Salmo truttaN Day
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
J Exp Biol 208:2683-92. 2005..These data have important implications for the design of experiments using fish that experience predictable, usually seasonal, changes in their natural environment (temperature, dissolved oxygen, changes in water levels, etc.)...
- Glycolysis and perinatal hypoxic-ischemic brain damageRobert C Vannucci
Department of Pediatrics, The Pennsylvania State University College of Medicine, The Milton S Hershey Medical Center, Hershey, PA, USA
Dev Neurosci 27:185-90. 2005..The major rate-limiting step for glycolysis was the transport of glucose across the blood-brain barrier into the brain...
- Downregulation of diaphragm electron transport chain and glycolytic enzyme gene expression in sepsisLeigh Ann Callahan
Div of Pulmonary and Critical Care Medicine, 1120 15th St, Rm BBR 5513, Medical College of Georgia, Augusta, GA 30912 3135, USA
J Appl Physiol (1985) 99:1120-6. 2005....
- A model of phosphofructokinase and glycolytic oscillations in the pancreatic beta-cellPål O Westermark
PSCI SANS, Nada, Royal Institute of Technology KTH, SE 100 44 Stockholm, Sweden
Biophys J 85:126-39. 2003..The theoretical results presented in this study apply to the study of insulin secretion mechanisms, since glycolytic oscillations have been proposed as a cause of oscillations in the ATP/ADP ratio which is linked to insulin secretion...
- Glycolytic enzyme activities are decreased during acute rejection in transplanted rat heartsE Bañón Maneus
Unitat de Bioquimica, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, Spain
Transplant Proc 37:4122-3. 2005..These results suggested that metabolic alterations in rejected hearts differ from other cardiomyopathies...
- The chemical form of selenium affects insulinomimetic properties of the trace element: investigations in type II diabetic dbdb miceAndreas S Mueller
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120, Heidelberg, Germany
J Nutr Biochem 14:637-47. 2003....
- Organization of metabolic pathways in vastus lateralis of patients with chronic obstructive pulmonary diseaseHoward J Green
Dept of Kinesiology, Univ of Waterloo, Waterloo, Ontario N2L 3G1, Canada
Am J Physiol Regul Integr Comp Physiol 295:R935-41. 2008..A comparison of enzyme ratios indicated greater potentials for glucose phosphorylation relative to the citric acid cycle and the electron transport chain and glycogenolysis and glycolysis relative to beta-oxidation...
- Quantitative elementary mode analysis of metabolic pathways: the example of yeast glycolysisJean Marc Schwartz
Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
BMC Bioinformatics 7:186. 2006....
- Control analysis as a tool to understand the formation of the las operon in Lactococcus lactisBrian Koebmann
Microbial Physiology and Genetics, BioCentrum DTU, Technical University of Denmark, Kgs Lyngby, Denmark
FEBS J 272:2292-303. 2005..Our analysis suggests that coregulation of PFK and PK provides a very efficient way to regulate glycolysis, and coregulating PK and LDH allows cells to maintain homolactic fermentation during glycolysis regulation...
- O(2)-dependent stimulation of the pentose phosphate pathway by S-nitrosocysteine in human erythrocytesFrancesco Misiti
Istituto di Biochimica e Biochimica Clinica, Facolta di Medicina e Chirurgia, Università Cattolica del Sacro Cuore L go F Vito 1, 00168 Rome, Italy
Biochem Biophys Res Commun 294:829-34. 2002..It takes into account the different route of catabolic degradation observed in vitro for GSNO under aerobic and anaerobic condition...
- Excess mannose limits the growth of phosphomannose isomerase PMI40 deletion strain of Saccharomyces cerevisiaeJuha Pekka Pitkänen
MediCel Ltd, Haartmaninkatu 8, FIN 00290 Helsinki, Finland
J Biol Chem 279:55737-43. 2004....
- Energy metabolism in the granulation tissue of diabetic rats during cutaneous wound healingAsheesh Gupta
Division of Pharmacology, Central Drug Research Institute, Lucknow, India
Mol Cell Biochem 270:71-7. 2005....
- Protective effect of Portulaca oleracea extracts on hypoxic nerve tissue and its mechanismWanyin Wang
Department of Naval Medicine, Second Military Medical University, Shanghai, China 200433
Asia Pac J Clin Nutr 16:227-33. 2007..We thus demonstrated that the PO extracts had protective effects on hypoxic nerve tissue...
- Inhibition of both Trypanosoma brucei bloodstream form and related glycolytic enzymes by a new kolavic acid derivative isolated from Entada abyssinicaB Nyasse
Laboratory of Bio Organic and Medicinal Chemistry, Department of Organic Chemistry, Faculty of Science, University of Yaounde I, Cameroon
Pharmazie 59:873-5. 2004..The new derivative showed a strong and selective inhibitory activity on the GAPDH enzyme of Trypanosoma brucei with an IC50 value of 0.012 mM...
- Manipulating training intensity and volume in already well-trained rats: effect on skeletal muscle oxidative and glycolytic enzymes and buffering capacityPaul B Laursen
School of Exercise, Biomedical and Health Sciences, Edith Cowan University, Building 19, Room 162, 100 Joondalup Drive, Joondalup, Western Australia 6027, Australia
Appl Physiol Nutr Metab 32:434-42. 2007..No differences were shown in fast-contracting muscle between HIT and CON...
- Sucrose synthase isoforms in cultured tobacco cellsSandra Matic
Department of Cell and Organism Biology, Lund University, Sölvegatan 35B, 22362 Lund, Sweden
Plant Physiol Biochem 42:299-306. 2004..Possible roles of these two isoforms in the sucrose turnover in BY-2 cells are discussed...
- Intracellular glucose and binding of hexokinase and phosphofructokinase to particulate fractions increase under hypoxia in heart of the amazonian armored catfish (Liposarcus pardalis)Jason R Treberg
Department of Biochemistry, Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
Physiol Biochem Zool 80:542-50. 2007..Such binding may be associated with increased glycolytic flux rates through modulation of enzyme-specific kinetics. The binding of HK and PFK occurs before any significant decrease in glycogen level...
- Glucose metabolism in the human preterm and term placenta of IUGR fetusesA L Magnusson
Department of Physiology and Pharmacology, Perinatal Center, Goteborg University, Medicinaregatan 11, Box 432, S 405 30 Goteborg, Sweden
Placenta 25:337-46. 2004..These results do not support the hypothesis of increased placental glucose consumption but suggest an altered glycolytic pathway in the IUGR placenta...
- Emetine inhibits glycolysis in isolated, perfused rat heartsShujia J Pan
Department of Kinesiology, University of Texas at Austin, Austin, TX, USA
Cardiovasc Toxicol 3:311-8. 2003..Homogenate centrifugation studies indicate that emetine acts on bound rather than unbound phosphofructokinase. The inhibition may be uncompetitive in nature...
- Conservation of glycolytic oscillations in Saccharomyces cerevisiae and human pancreatic beta-cells: a study of metabolic robustnessA S Silva
Laboratorio de Biologia Molecular, Centro Infantil Boldrini, Campinas, SP, Brasil
Genet Mol Res 5:525-35. 2006....
- Increased muscle oxidative potential following resistance training induced fibre hypertrophy in young menJason E Tang
Department of Kinesiology, Exercise Metabolism Research Group, McMaster University, 1280 Main St W, Hamilton, ON L8S 4K1, Canada
Appl Physiol Nutr Metab 31:495-501. 2006..We conclude that resistance training provides a stimulus for improving muscle oxidative potential, as reflected by the increased activities of CS and beta-HAD following resistance training induced hypertrophy...
- Analysis of in vivo kinetics of glycolysis in aerobic Saccharomyces cerevisiae by application of glucose and ethanol pulsesDiana Visser
PURAC, P O Box 21, 4200 AA Gorinchem, The Netherlands
Biotechnol Bioeng 88:157-67. 2004..Our results confirm that FBP stimulates pyruvate kinase and suggest that this effect is pronounced. Furthermore, it appears that PEP does not play an important role in the allosteric regulation of phosphofructo kinase...
- Differing mechanisms of cold-induced changes in capillary supply in m. tibialis anterior of rats and hamstersDurmus Deveci
Department of Physiology, Cumhuriyet University, 58140 Sivas, Turkey
J Exp Biol 205:829-40. 2002..These responses are consistent with the strategies of resisting and accommodating, respectively, an annual fall in environmental temperature...
- Different modes of activating phosphofructokinase, a key regulatory enzyme of glycolysis, in working vertebrate muscleG Wegener
Institut fur Zoologie, Molekulare Physiologie, Johannes Gutenberg Universitat, Saarstrasse 21, D 55099 Mainz, Germany
Biochem Soc Trans 30:264-70. 2002..Whether this phenomenon exists in vertebrates other than the frog, and maybe even in humans, and how the content of F2,6P(2)in muscle is controlled are intriguing open questions...
- Reconstructing the mosaic glycolytic pathway of the anaerobic eukaryote MonocercomonoidesNatalia A Liapounova
Department of Biological Sciences, The University of Calgary, Calgary, Alberta T2N 1N4, Canada
Eukaryot Cell 5:2138-46. 2006....
- Acute responses in muscle mitochondrial and cytosolic enzyme activities during heavy intermittent exerciseH J Green
Dept of Kinesiology, Univ of Waterloo, Waterloo, ON, Canada
J Appl Physiol (1985) 104:931-7. 2008....
- Suspected utility of enzymes with multiple activities in the small genome Mycoplasma species: the replacement of the missing "household" nucleoside diphosphate kinase gene and activity by glycolytic kinasesJ Dennis Pollack
Department of Molecular Virology, Immunology, and Medical Genetics, The College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210, USA
OMICS 6:247-58. 2002..capricolum Kid(T) that their 6-phosphofructokinases (6-PFKs), phosphoglycerate kinases (PGKs), pyruvate kinases (PKs), and acetate kinases (AKs), besides ..
- Entamoeba histolytica: computer-assisted modeling of phosphofructokinase for the prediction of broad-spectrum antiparasitic agentsC L Byington
Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City 84132, USA
Exp Parasitol 87:194-202. 1997..The enzyme differs significantly from ATP-dependent phosphofructokinases found in humans and as such represents an important drug target...
- Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricusP Branny
Laboratoire d Enzymologie du Centre National de la Recherche Scientifique, Gif sur Yvette, France
J Bacteriol 175:5344-9. 1993..The amino acid sequence of this enzyme was homologous to those of the ATP-dependent phosphofructokinases from E...
- Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinaseHideaki Tsuge
Institute for Health Sciences, Tokushima Bunri University, Yamashiro cho, Japan
Protein Sci 11:2456-63. 2002..Those are ADP-dependent glucokinases (ADPGK) and phosphofructokinases in their glycolytic pathway. Here, we succeeded in gene cloning the ADPGK from P...
- ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaeaC H Verhees
Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen, The Netherlands
J Bacteriol 183:7145-53. 2001..Interestingly, uncharacterized homologs of this unusual kinase are present in several eucarya...
- The structure of the ATP-bound state of S. cerevisiae phosphofructokinase determined by cryo-electron microscopyMONTSERRAT BARCENA
University of Vermont, College of Medicine, Department of Molecular Physiology and Biophysics, Burlington, VT 05405, USA
J Struct Biol 159:135-43. 2007..Eukaryotic phosphofructokinases exhibit a far more sophisticated regulatory mechanism, they are more complex structures regulated by a ..
- Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosusJ E Tuininga
Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands
J Biol Chem 274:21023-8. 1999..the reverse reaction, nor was it regulated by any of the known allosteric modulators of ATP-dependent phosphofructokinases. ATP and AMP were identified as competitive inhibitors of the phosphofructokinase, raising the K(m) for ADP ..
- Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family memberRafael E Parducci
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
Biochemistry 45:9291-9. 2006..Inorganic phosphate, typically considered an activator of adenosine kinases, ribokinases, and phosphofructokinases (nonhomologous to Pfk-2) acted as an inhibitor of wild-type and E190Q mutant Pfk-2...
- Structures of S. pombe phosphofructokinase in the F6P-bound and ATP-bound statesShaun Benjamin
University of Vermont, College of Medicine, Department of Molecular Physiology and Biophysics, Burlington, VT 05405, USA
J Struct Biol 159:498-506. 2007..pombe subunit, the oligomeric organization of subunits in this enzyme is different from other yeast phosphofructokinases. Electron microscopy studies were carried out to reveal the quaternary structure of the homo-octameric Pfk1 ..
- A novel form of 6-phosphofructokinase. Identification and functional relevance of a third type of subunit in Pichia pastorisKatrin Tanneberger
Institute of Biochemistry, Molecular Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
J Biol Chem 282:23687-97. 2007Classically, 6-phosphofructokinases are homo- and hetero-oligomeric enzymes consisting of alpha subunits and alpha/beta subunits, respectively...
- Glucose catabolism of Escherichia coli strains with increased activity and altered regulation of key glycolytic enzymesM Emmerling
Institute of Biotechnology, Eidgenossische Technische Hochschule Zurich, Switzerland
Metab Eng 1:117-27. 1999..Homologous and heterologous pyruvate kinases (Pyk) and phosphofructokinases (Pfk) were individually and simultaneously overexpressed. Overexpression of the E...
- 6-phosphofructokinase from Pichia pastoris: purification, kinetic and molecular characterization of the enzymeJürgen Kirchberger
Institut fur Biochemie, Medizinische Fakultät der Universität Leipzig, Liebigstrasse 16, D 04103 Leipzig, Germany
Yeast 19:933-47. 2002..structural organisation of the active enzyme seems to be different in comparison with hetero-octameric 6-phosphofructokinases from other yeast species...
- Purification and properties of the pyrophosphate-dependent phosphofructokinase from Dictyoglomus thermophilum Rt46 B.1Y H Ding
Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand
Extremophiles 3:131-7. 1999..The enzyme was not allosterically controlled by traditional modulators of phosphofructokinases and has significant activity with tripolyphosphate and polyphosphate...
- Purification and characterization of an ADP-dependent phosphofructokinase from Thermococcus zilligiiR S Ronimus
Thermophile Research Unit, University of Waikato, Hamilton, New Zealand
Extremophiles 3:121-9. 1999..56mM (apparent Vmax of 2.9Umg(-1) and 12.5mM, respectively. The biochemical characteristics of this unique ADP-dependent enzymatic activity are compared to ATP and pyrophosphate-dependent phosphofructokinases.
- Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12Y Yang
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030 1501, USA
FEMS Microbiol Lett 141:89-95. 1996..acid sequence of PdxK has signature motifs of the PfkB superfamily of carbohydrate kinases, which includes phosphofructokinases and ribokinases, and suggests that three unidentified ORFs of Salmonella typhimurium, Haemophilus ..
- Diacylglyceride kinases, sphingosine kinases and NAD kinases: distant relatives of 6-phosphofructokinasesGilles Labesse
Centre de Biochimie Structurale, INSERM U554 CNRS UMR5048, Universite Montpellier I, 15 Avenue Charles Flahault, 34060 Montpellier Cedex, France
Trends Biochem Sci 27:273-5. 2002Diacylglyceride kinases, sphingosine kinases, NAD kinases and 6-phosphofructokinases are thought to be related despite large evolution of their sequences...
- Purification, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzymeChristian Winkler
Institute of Botany III, University of Dusseldorf, Germany
FEBS J 274:429-38. 2007..that a large family of genomics-derived higher plant sequences currently annotated in the databases as putative pyrophosphate-dependent phosphofructokinases according to sequence similarity is misannotated with respect to the cosubstrate.
- Mapping of glycolytic enzyme-binding sites on human erythrocyte band 3Haiyan Chu
Chemistry Department, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA
Biochem J 400:143-51. 2006..Taken together, these results argue that band 3 provides a nucleation site for the GE complex on the human erythrocyte membrane and that other components near band 3 must also participate in organizing the enzyme complex...
- Expression of caveolin-1 in lymphocytes induces caveolae formation and recruitment of phosphofructokinase to the plasma membraneJohana Vallejo
Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65212, USA
FASEB J 19:586-7. 2005..We therefore conclude that CAV-1 functions as a scaffolding protein for PFK and that this may contribute to the elucidation of the basis for carbohydrate compartmentation to the plasma membrane in a wide variety of cell types...
- Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolismNikolai Nordsborg
Institute of Exercise and Sports Sciences, August Krogh Institute, 2100 Copenhagen, Denmark
J Appl Physiol 95:1201-6. 2003..In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished...
- Hemolysis, myopathy, and cardiac disease associated with hereditary phosphofructokinase deficiency in two WhippetsKaren Gerber
Axiom Veterinary Laboratory, Newton Abbott, UK
Vet Clin Pathol 38:46-51. 2009..In addition to causing hemolysis and exertional myopathy, heart disease may be a prominent clinical component of PFK deficiency in this breed and has not been previously recognized in PFK-deficient English Springer Spaniels...
- NAD kinases use substrate-assisted catalysis for specific recognition of NADGuillaume Poncet-Montange
Atelier de Bio et Chimie Informatique Structurale, Centre de Biochimie Structurale, UMR 5048 CNRS, Universites Montpellier 1 et 2, 29 rue de Navacelles, Montpellier, France
J Biol Chem 282:33925-34. 2007..and directed mutagenesis have previously shown that NAD kinases (NADKs) and the distantly related 6-phosphofructokinases share the same catalytically important GGDGT motif...
- Diabetes causes marked changes in function and metabolism of rat neutrophilsT C Alba-Loureiro
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof Lineu Prestes 1524, 05508 900 São Paulo SP, Brazil
J Endocrinol 188:295-303. 2006..The treatment with insulin abolished the changes induced by the diabetic state even with no marked change in glycemia. Therefore, insulin may have a direct effect on neutrophil metabolism and function...
- Drug effects on drug targets: inhibition of enzymes by neuroleptics, antimycotics, antibiotics and other drugs on human pathogenic amoebas and their anti-proliferative effectsRAUL N ONDARZA
Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico UNAM, University City, Mexico 04510, Mexico
Recent Pat Antiinfect Drug Discov 2:206-16. 2007....
- The first crystal structure of phosphofructokinase from a eukaryote: Trypanosoma bruceiJose Martinez-Oyanedel
Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh EH9 3JR, Scotland
J Mol Biol 366:1185-98. 2007....
- Mathematical simulation of membrane processes and metabolic fluxes of the pancreatic beta-cellFrank Diederichs
Marschweg 10, D 29690 Schwarmstedt, Germany
Bull Math Biol 68:1779-818. 2006..The simulation shows that the so-called pyruvate paradox possibly results from a relatively low membrane conductance of beta-cells for pyruvate...
- Combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epitheliaLiviu Badea
Bioinformatics Group, National Institute for Research in Informatics, Bucharest 011455, Romania
Hepatogastroenterology 55:2016-27. 2008..To address this problem, laser microdissection studies have been performed, but these have to deal with much smaller tissue sample quantities and therefore have significantly higher experimental noise...
- Regulation of the central glycolytic genes in Bacillus subtilis: binding of the repressor CggR to its single DNA target sequence is modulated by fructose-1,6-bisphosphateThierry Doan
Génétique Moléculaire et Cellulaire, INRA UMR216 CNRS URA1925 and INAP G, F 78850 Thiverval Grignon, France
Mol Microbiol 47:1709-21. 2003..Genomic sequence comparisons suggest that these results can apply to numerous low-G+C, Gram-positive bacterial species...
- Functional maps of the junctions between interglobular contacts and active sites in glycolytic enzymes -- a comparative analysis of the biochemical and structural dataIvan Y Torshin
Laboratory of Kinetics and Catalysis, Chemical Department of Moscow State University, Moscow, Russia
Med Sci Monit 8:BR123-35. 2002..Since the conformation of an enzyme determines its catalytic properties, the structural mechanism(s) influencing the activity is of considerable interest...
- Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cellsAnna Karin Gustavsson
Department of Physics, University of Gothenburg, Sweden
FEBS J 281:2784-93. 2014..The oscillatory manifold could be approximated by allosteric control values of phosphofructokinase for ATP and AMP...
- An in vivo approach to isolating allosteric pathways using hybrid multimeric proteinsCuijuan Tie
Department of Biochemistry and Biophysics, Texas A and M University and Texas AgriLife Research, College Station, TX, USA
Methods Mol Biol 796:307-15. 2012..The in vivo method does increase the yield of the hybrids produced while decreasing the time required for their isolation...
- Ventricle-specific metabolic differences in the newborn piglet myocardium in vivo and during arrested global ischemiaDanny Quaglietta
Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
Pediatr Res 63:15-9. 2008..This profile may offer one explanation for greater LV-dysfunction relative to the RV in children following ischemia...
- The recovery of dipolar relaxation times from fluorescence decays as a tool to probe local dynamics in single tryptophan proteinsGiampiero Mei
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome 00133, Italy
Arch Biochem Biophys 417:159-64. 2003..These findings may have important implications on the analysis of small-scale protein dynamics, since dielectric relaxation directly probes a local structural change around the excited state of tryptophan...
- Expression of phosphofructokinase in Neisseria meningitidisGino J E Baart
Netherlands Vaccine Institute NVI, Unit Research and Development, PO Box 457, 3720 AL Bilthoven, The Netherlands
Microbiology 156:530-42. 2010....
- Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophilaR Ronimus
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand
Arch Microbiol 175:308-12. 2001..Phylogenetic analysis demonstrated a close relationship between the thermophilic S. thermophila phosphofructokinase and the large beta-subunits of the phosphofructokinases from Borrelia burgdorferi and Treponema pallidum.
- The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetasesColin P Kenyon
CSIR, Biosciences, Meiring Naude Road, Pretoria, 0001, Gauteng, South Africa
BMC Biochem 12:36. 2011..4 μM to in excess of 1000 μM for ATP. It is not known how this diverse range of enzymes mechanistically achieves the regulation of catalysis via an affinity range for ATP varying by three-orders of magnitude...
- From genome to enzyme: analysis of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium Synechocystis sp. PCC 6803Vicki L Knowles
Department of Biology, Queen s University, Kingston, Ontario, K7L 3N6 Canada
Plant Cell Physiol 44:758-63. 2003..Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated)...
- Redefining the role of the quaternary shift in Bacillus stearothermophilus phosphofructokinaseRockann Mosser
Department of Biochemistry and Biophysics, Texas A and M University, and Texas A and M AgriLife Research, College Station, TX 77843 2128, USA
Biochemistry 52:5421-9. 2013..The results suggest that the main role of the quaternary shift may be to influence ligand binding and not to cause the heterotropic allosteric inhibition per se. ..
- Control of glycolysis in mature boar spermatozoa: effect of pH in vitroA R Jones
School of Molecular and Microbial Biosciences, Discipline of Biochemistry, The University of Sydney, Sydney, NSW, Australia
Reprod Fertil Dev 16:319-24. 2004..Phosphofructokinase has been shown to be sensitive to this drop in pH rather than to the build up of lactate ions or ATP, thereby controlling the rate of glycolysis in vitro...
- Quantification of allosteric influence of Escherichia coli phosphofructokinase by frequency domain fluorescenceAudrey S Pham
Division of Pathology and Laboratory Medicine, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
Biophys J 85:656-66. 2003..Coupling interactions giving rise to positive entropy components are manifested by increasing flexibility of the ternary complexes rather than the binary complexes...
- A broad specificity nucleoside kinase from Thermoplasma acidophilumSarah R Elkin
Department of Chemistry, University of Virginia, Charlottesville, VA 22904 4319, USA
Proteins 81:568-82. 2013..PfkB family of carbohydrate kinases (a family member of the Ribokinase clan) which include ribokinases, 1-phosphofructokinases, 6-phosphofructo-2-kinase, inosine/guanosine kinases, fructokinases, adenosine kinases, and many more...
- A metronome-like control of the calcium signal leading to nuclear envelope breakdown and mitosis in sand dollar (Echinaracnius parma) cellsRobert B Silver
Department of Pharmacology, School of Medicine, Wayne State University, Detroit, Michigan, USA
Biol Bull 203:213-5. 2002
- Wavenumber distribution in Hopf-wave instability: the reversible Selkov model of glycolytic oscillationArun K Dutt
Faculty of Computing, Engineering and Mathematical Sciences, Du Pont Building, University of the West of England, Frenchay Campus, Bristol BS16 1QY, United Kingdom
J Phys Chem B 109:17679-82. 2005....
- Expression, purification, crystallization and preliminary crystallographic analysis of Trypanosoma brucei phosphofructokinaseJeffrey W Keillor
Departement de Chimie, Universite de Montreal, CP 6128, Succursale Centre Ville, Montreal, Québec H3C 1J7, Canada
Acta Crystallogr D Biol Crystallogr 59:532-4. 2003..The availability of this first eukaryotic PFK structure will be of particular significance for structure-based drug design and will also provide important additional structural evidence for the allosteric control of PFK activity...
- Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeastGuillermo G Zampar
Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
Mol Syst Biol 9:651. 2013..Taken together, our results represent an important contribution toward a systems-level understanding of how this adaptation is realized...
- Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureusL M Orchard
Department of Biochemistry, University of Cambridge, U K
Proc Biol Sci 242:87-90. 1990..coli (pfkA) and the 6-phosphofructokinases present in a number of pro- and eukaryotic organisms, but there is 27%, 25% and 22% identify of sequence ..
- The HIF-1 response to simulated ischemia in mouse skeletal muscle cells neither enhances glycolysis nor prevents myotube cell deathNathalie Dehne
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstr 55, D 45122 Essen, Germany
Am J Physiol Regul Integr Comp Physiol 293:R1693-701. 2007..In contrast, myoblasts survive ischemia and thus may play an important role during regeneration and HIF-1-induced revascularization...
- Effect of ethylene treatment on the concentration of fructose-2,6-bisphosphate and on the activity of phosphofructokinase 2/fructose-2,6-bisphosphatase in bananaE Mertens
Laboratoire de Chimie Physiologique, Universite Catholique de Louvain, Bruxelles, Belgium
Eur J Biochem 167:579-83. 1987..enzyme, but also by an early increase in the concentration of fructose 6-phosphate, the substrate of all phosphofructokinases, and also by the decrease in the concentration of glycerate 3-phosphate, a potent inhibitor of ..
- Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strainsYipeng Wang
Department of Biochemistry and Cell Biology, MS 140, Rice University, 6100 Main Street, Houston, TX 77005 1892, USA
Appl Microbiol Biotechnol 97:6883-93. 2013..The results indicated that the flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis. ..
- An essential methionine residue involved in substrate binding by phosphofructokinasesX Wang
Department of Microbiology, Chicago Medical School, Illinois 60064, USA
Biochem Biophys Res Commun 250:466-8. 1998An alignment of all PPi-dependent phosphofructokinases and all allosteric ATP-dependent PFKs shows relatively few residues that are fully conserved...
- Differential gene expression in neutrophils from patients with generalized aggressive periodontitisT Kubota
Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata, Japan
J Periodontal Res 36:390-7. 2001..Neutrophil functions in Aggressive-P patients are suggested to be altered by regulatory factors of the immune system including HSF4b (transcription factor), Zf9 (activator of TGF-beta) and muskelin (cellular adhesion)...
- Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humansMartin Thomassen
Department of Nutrition, Exercise and Sports, Section of Integrated Physiology, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2 sal, 2100 Copenhagen, Denmark
J Physiol 591:1523-33. 2013..This, together with the observation of a higher abundance of the Na(+)-K(+) pump α2 isoform protein in type II fibres, is likely to have importance for the exercise-induced human Na(+)-K(+) pump activity in the different fibre types...
- Neuroprotection conferred by post-ischemia ethanol therapy in experimental stroke: an inhibitory effect on hyperglycolysis and NADPH oxidase activationRyan Kochanski
Department of Neurosurgery, Wayne State University School of Medicine, Detroit, Michigan, USA
J Neurochem 126:113-21. 2013..Because of the lack of associated hypoglycemia and selectivity toward decreasing cerebral metabolism, further investigation of ethanol's use as a post-stroke therapy, especially in the context of hyperglycemia, seems warranted...
- Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traitsAngelo Scuteri
Unità Operativa Geriatria, Istituto per la Patologia Endocrina e Metabolica, Rome, Italy
PLoS Genet 3:e115. 2007..These changes could have a significant impact on the risk of obesity-related morbidity in the general population...
- Genetic polymorphism in Dirofilaria immitisCatherine Bourguinat
Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte Anne de Bellevue, H9X3V9 Quebec, Canada
Vet Parasitol 176:368-73. 2011..Differences in F coefficients were observed between the groups. D. immitis was found to be genetically heterogeneous. This genetic heterogeneity has implication for the development of genetically selected strains of the parasite...
- Metabolic and transcriptomic adaptation of Lactococcus lactis subsp. lactis Biovar diacetylactis in response to autoacidification and temperature downshift in skim milkSandy Raynaud
Laboratoire Biotechnologie Bioprocédés, UMR 5504 INSA CNRS and UMR 792 INSA INRA, Institut National des Sciences Appliquees, 135 Avenue de Rangueil, 31077 Toulouse Cedex 4, France
Appl Environ Microbiol 71:8016-23. 2005..If specific responses to acid and cold stresses were identified, this exhaustive analysis also enabled induction of unexpected pathways to be shown...
- STRUCTURE AND EXPRESSION OF THE PARAMYXOVIRUS SV5 GENOMERobert A Lamb; Fiscal Year: 2010..We will elucidate the role of mutations in the F protein in the context of a virus infection by using reverse genetics and we will study fusion activity, virus growth and virus assembly of the altered viruses. ..