phosphofructokinases

Summary

Summary: Allosteric enzymes that regulate glycolysis and gluconeogenesis. These enzymes catalyze phosphorylation of fructose-6-phosphate to either fructose-1,6-bisphosphate (PHOSPHOFRUCTOKINASE-1 reaction), or to fructose-2,6-bisphosphate (PHOSPHOFRUCTOKINASE-2 reaction).

Top Publications

  1. pmc Characterization of glycolytic enzyme interactions with murine erythrocyte membranes in wild-type and membrane protein knockout mice
    M Estela Campanella
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Blood 112:3900-6. 2008
  2. ncbi Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources
    Michelle R Lovingshimer
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Protein Expr Purif 46:475-82. 2006
  3. pmc Existence of a true phosphofructokinase in Bacillus sphaericus: cloning and sequencing of the pfk gene
    Alejandro F Alice
    Laboratorio de Microbiologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
    Appl Environ Microbiol 68:6410-5. 2002
  4. ncbi On the mechanisms of glycolytic oscillations in yeast
    Mads F Madsen
    Functional Dynamics Group, Department of Chemistry, University of Copenhagen, Denmark
    FEBS J 272:2648-60. 2005
  5. ncbi Yeast glycolytic oscillations that are not controlled by a single oscillophore: a new definition of oscillophore strength
    Karin A Reijenga
    Department of Molecular Cell Physiology, CRbCS, BioCentrum Amsterdam, Faculty of Earth and Life Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    J Theor Biol 232:385-98. 2005
  6. ncbi The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases
    Iain W McNae
    Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh, Scotland
    J Mol Biol 385:1519-33. 2009
  7. ncbi The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle
    Katarzyna Banaszak
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
    J Mol Biol 407:284-97. 2011
  8. pmc Turing patterns inside cells
    Damián E Strier
    Service de Chimie Physique and Center for Nonlinear Phenomena and Complex Systems, Universite Libre de Bruxelles, Brussels, Belgium
    PLoS ONE 2:e1053. 2007
  9. ncbi Glycolytic oscillations and waves in an open spatial reactor: Impact of feedback regulation of phosphofructokinase
    Satenik Bagyan
    Institute of Experimental Physic, Group of Biophysics, Otto von Guericke University of Magdeburg, Universitatsplatz 2, D 39106 Magdeburg, Germany
    Biophys Chem 116:67-76. 2005
  10. pmc Inward rotating spiral waves in glycolysis
    Ronny Straube
    Max Planck Institute for Dynamics of Complex Technical Systems, Systems Biology Group, Sandtorstr 1, Magdeburg, Germany
    Biophys J 99:L4-6. 2010

Research Grants

Detail Information

Publications180 found, 100 shown here

  1. pmc Characterization of glycolytic enzyme interactions with murine erythrocyte membranes in wild-type and membrane protein knockout mice
    M Estela Campanella
    Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Blood 112:3900-6. 2008
    ..These data suggest that oxygenation-dependent assembly of GEs on the membrane could be a general phenomenon of mammalian erythrocytes and that stability of these interactions depends primarily on band 3...
  2. ncbi Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources
    Michelle R Lovingshimer
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Protein Expr Purif 46:475-82. 2006
    ..In addition to having both pfkA and pfkB deleted, RL257 contains the lacI(q) allele, which allows for inducible expression when coupled with an expression vector containing either the lac or tac promoter...
  3. pmc Existence of a true phosphofructokinase in Bacillus sphaericus: cloning and sequencing of the pfk gene
    Alejandro F Alice
    Laboratorio de Microbiologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
    Appl Environ Microbiol 68:6410-5. 2002
    ..A careful sequence analysis revealed the conservation of different catalytic and regulatory residues, as well as the enzyme's phylogenetic affiliation with the family of allosteric ATP-PFK enzymes...
  4. ncbi On the mechanisms of glycolytic oscillations in yeast
    Mads F Madsen
    Functional Dynamics Group, Department of Chemistry, University of Copenhagen, Denmark
    FEBS J 272:2648-60. 2005
    ....
  5. ncbi Yeast glycolytic oscillations that are not controlled by a single oscillophore: a new definition of oscillophore strength
    Karin A Reijenga
    Department of Molecular Cell Physiology, CRbCS, BioCentrum Amsterdam, Faculty of Earth and Life Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    J Theor Biol 232:385-98. 2005
    ..These results constitute proof that glycolytic oscillations in yeast are not caused by a single oscillophore and provide a new, subtle, definition for the oscillophore strength of an enzyme...
  6. ncbi The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases
    Iain W McNae
    Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh, Scotland
    J Mol Biol 385:1519-33. 2009
    ..brucei PFK compared with bacterial and mammalian PFKs give optimism for the discovery of species-specific drugs for the treatment of diseases caused by protist parasites of the trypanosomatid family...
  7. ncbi The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle
    Katarzyna Banaszak
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
    J Mol Biol 407:284-97. 2011
    ..Crystallographic results have been used as the basis for structure-based effector design...
  8. pmc Turing patterns inside cells
    Damián E Strier
    Service de Chimie Physique and Center for Nonlinear Phenomena and Complex Systems, Universite Libre de Bruxelles, Brussels, Belgium
    PLoS ONE 2:e1053. 2007
    ..Turing patterns inside cells could provide a check-point that combines mechanical and biochemical information to trigger events during the cell division process...
  9. ncbi Glycolytic oscillations and waves in an open spatial reactor: Impact of feedback regulation of phosphofructokinase
    Satenik Bagyan
    Institute of Experimental Physic, Group of Biophysics, Otto von Guericke University of Magdeburg, Universitatsplatz 2, D 39106 Magdeburg, Germany
    Biophys Chem 116:67-76. 2005
    ..The generation of travelling NADH-waves is observed in the diffusive layer, when feeding the yeast extract with substrates. Break-up of circular-shaped waves is repeatedly observed, resulting in the formation of rotating NADH-spirals...
  10. pmc Inward rotating spiral waves in glycolysis
    Ronny Straube
    Max Planck Institute for Dynamics of Complex Technical Systems, Systems Biology Group, Sandtorstr 1, Magdeburg, Germany
    Biophys J 99:L4-6. 2010
    ..Numerical simulations with an allosteric model for the phosphofructokinase can reproduce these inward propagating waves over a wide range of parameters if the octameric structure of yeast phosphofructokinase is taken into account...
  11. ncbi The biochemical properties and phylogenies of phosphofructokinases from extremophiles
    R S Ronimus
    Thermophile Research Unit, Department of Biological Sciences, University of Waikato, Hamilton, New Zealand
    Extremophiles 5:357-73. 2001
    ..the crenarchaeal pyrophosphate-dependent PFK of Thermoprotetus tenax) and plant pyrophosphate-dependent phosphofructokinases; the PFKB family, exemplified by the minor ATP-dependent PFK activity of Escherichia coli (PFK 2), but ..
  12. ncbi Glycolytic sequence and respiration of Debaryomyces hansenii as compared to Saccharomyces cerevisiae
    N S Sánchez
    Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Circuito exterior s n, Ciudad Universitaria, D F 04510, Mexico
    Yeast 23:361-74. 2006
    ..cerevisiae but not in D. hansenii. D. hansenii shows an alternative oxidase, which in our experiments did not appear to be coupled to the production of ATP...
  13. ncbi Ethanolic extract of Ocimum sanctum leaves partially attenuates streptozotocin-induced alterations in glycogen content and carbohydrate metabolism in rats
    V Vats
    Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110049, India
    J Ethnopharmacol 90:155-60. 2004
    ..OS did not affect glycogen content in any tissue. Activity of HK, GK and PFK in diabetic controls was 35, 50 and 60% of the controls and OS partially corrected this alteration...
  14. ncbi Detachment of glycolytic enzymes from cytoskeleton of Lewis lung carcinoma and colon adenocarcinoma cells induced by clotrimazole and its correlation to cell viability and morphology
    Julia Penso
    Health Sciences Research Center, Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Mol Genet Metab 76:181-8. 2002
    ....
  15. ncbi Shift in metabolic fuel in acylation-stimulating protein-deficient mice following a high-fat diet
    Christian Roy
    Centre de recherche Hopital Laval, Y 2186, Chemin Ste Foy, QC, Canada
    Am J Physiol Endocrinol Metab 294:E1051-9. 2008
    ..However, these in vivo data suggest that ASP inhibition could be a potentially viable approach in correcting muscle metabolic dysfunction in obesity...
  16. ncbi Significant increase of glycolytic flux in Torulopsis glabrata by inhibition of oxidative phosphorylation
    Liming Liu
    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, China
    FEMS Yeast Res 6:1117-29. 2006
    ..9967) and the average specific pyruvate production rate (R2=0.965) were closely correlated with the intracellular ATP level, all of them being increased at a lower intracellular ATP level...
  17. ncbi Design, synthesis and trypanocidal activity of lead compounds based on inhibitors of parasite glycolysis
    Matthew W Nowicki
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK
    Bioorg Med Chem 16:5050-61. 2008
    ..Trypanocidal activity also showed potency in the low micromolar range and confirms these inhibitors as promising candidates for the development towards the design of anti-trypanosomal drugs...
  18. ncbi [An experimental study of the changes of rat hepatocytic glycolysis during hypoxia]
    Zhengwei Ma
    Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, P R China
    Zhonghua Shao Shang Za Zhi 18:238-41. 2002
    ..To investigate the effects of hypoxia on the glycolysis in cultured rat hepatocytes...
  19. ncbi Effect of feeding aqueous extract of Pterocarpus marsupium on glycogen content of tissues and the key enzymes of carbohydrate metabolism
    Jagdish Kumari Grover
    Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India
    Mol Cell Biochem 241:53-9. 2002
    ..Activity of HK, GK and PFK in diabetic controls was 35,50 and 60% of the controls and PM completely corrected this alteration in PFK and only partly in HK and GK...
  20. ncbi The effects of acclimation to reversed seasonal temperatures on the swimming performance of adult brown trout Salmo trutta
    N Day
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Exp Biol 208:2683-92. 2005
    ..These data have important implications for the design of experiments using fish that experience predictable, usually seasonal, changes in their natural environment (temperature, dissolved oxygen, changes in water levels, etc.)...
  21. ncbi Glycolysis and perinatal hypoxic-ischemic brain damage
    Robert C Vannucci
    Department of Pediatrics, The Pennsylvania State University College of Medicine, The Milton S Hershey Medical Center, Hershey, PA, USA
    Dev Neurosci 27:185-90. 2005
    ..The major rate-limiting step for glycolysis was the transport of glucose across the blood-brain barrier into the brain...
  22. ncbi Downregulation of diaphragm electron transport chain and glycolytic enzyme gene expression in sepsis
    Leigh Ann Callahan
    Div of Pulmonary and Critical Care Medicine, 1120 15th St, Rm BBR 5513, Medical College of Georgia, Augusta, GA 30912 3135, USA
    J Appl Physiol (1985) 99:1120-6. 2005
    ....
  23. pmc A model of phosphofructokinase and glycolytic oscillations in the pancreatic beta-cell
    Pål O Westermark
    PSCI SANS, Nada, Royal Institute of Technology KTH, SE 100 44 Stockholm, Sweden
    Biophys J 85:126-39. 2003
    ..The theoretical results presented in this study apply to the study of insulin secretion mechanisms, since glycolytic oscillations have been proposed as a cause of oscillations in the ATP/ADP ratio which is linked to insulin secretion...
  24. ncbi Glycolytic enzyme activities are decreased during acute rejection in transplanted rat hearts
    E Bañón Maneus
    Unitat de Bioquimica, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, Spain
    Transplant Proc 37:4122-3. 2005
    ..These results suggested that metabolic alterations in rejected hearts differ from other cardiomyopathies...
  25. ncbi The chemical form of selenium affects insulinomimetic properties of the trace element: investigations in type II diabetic dbdb mice
    Andreas S Mueller
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120, Heidelberg, Germany
    J Nutr Biochem 14:637-47. 2003
    ....
  26. ncbi Organization of metabolic pathways in vastus lateralis of patients with chronic obstructive pulmonary disease
    Howard J Green
    Dept of Kinesiology, Univ of Waterloo, Waterloo, Ontario N2L 3G1, Canada
    Am J Physiol Regul Integr Comp Physiol 295:R935-41. 2008
    ..A comparison of enzyme ratios indicated greater potentials for glucose phosphorylation relative to the citric acid cycle and the electron transport chain and glycogenolysis and glycolysis relative to beta-oxidation...
  27. pmc Quantitative elementary mode analysis of metabolic pathways: the example of yeast glycolysis
    Jean Marc Schwartz
    Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    BMC Bioinformatics 7:186. 2006
    ....
  28. ncbi Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis
    Brian Koebmann
    Microbial Physiology and Genetics, BioCentrum DTU, Technical University of Denmark, Kgs Lyngby, Denmark
    FEBS J 272:2292-303. 2005
    ..Our analysis suggests that coregulation of PFK and PK provides a very efficient way to regulate glycolysis, and coregulating PK and LDH allows cells to maintain homolactic fermentation during glycolysis regulation...
  29. ncbi O(2)-dependent stimulation of the pentose phosphate pathway by S-nitrosocysteine in human erythrocytes
    Francesco Misiti
    Istituto di Biochimica e Biochimica Clinica, Facolta di Medicina e Chirurgia, Università Cattolica del Sacro Cuore L go F Vito 1, 00168 Rome, Italy
    Biochem Biophys Res Commun 294:829-34. 2002
    ..It takes into account the different route of catabolic degradation observed in vitro for GSNO under aerobic and anaerobic condition...
  30. ncbi Excess mannose limits the growth of phosphomannose isomerase PMI40 deletion strain of Saccharomyces cerevisiae
    Juha Pekka Pitkänen
    MediCel Ltd, Haartmaninkatu 8, FIN 00290 Helsinki, Finland
    J Biol Chem 279:55737-43. 2004
    ....
  31. ncbi Energy metabolism in the granulation tissue of diabetic rats during cutaneous wound healing
    Asheesh Gupta
    Division of Pharmacology, Central Drug Research Institute, Lucknow, India
    Mol Cell Biochem 270:71-7. 2005
    ....
  32. ncbi Protective effect of Portulaca oleracea extracts on hypoxic nerve tissue and its mechanism
    Wanyin Wang
    Department of Naval Medicine, Second Military Medical University, Shanghai, China 200433
    Asia Pac J Clin Nutr 16:227-33. 2007
    ..We thus demonstrated that the PO extracts had protective effects on hypoxic nerve tissue...
  33. ncbi Inhibition of both Trypanosoma brucei bloodstream form and related glycolytic enzymes by a new kolavic acid derivative isolated from Entada abyssinica
    B Nyasse
    Laboratory of Bio Organic and Medicinal Chemistry, Department of Organic Chemistry, Faculty of Science, University of Yaounde I, Cameroon
    Pharmazie 59:873-5. 2004
    ..The new derivative showed a strong and selective inhibitory activity on the GAPDH enzyme of Trypanosoma brucei with an IC50 value of 0.012 mM...
  34. ncbi Manipulating training intensity and volume in already well-trained rats: effect on skeletal muscle oxidative and glycolytic enzymes and buffering capacity
    Paul B Laursen
    School of Exercise, Biomedical and Health Sciences, Edith Cowan University, Building 19, Room 162, 100 Joondalup Drive, Joondalup, Western Australia 6027, Australia
    Appl Physiol Nutr Metab 32:434-42. 2007
    ..No differences were shown in fast-contracting muscle between HIT and CON...
  35. ncbi Sucrose synthase isoforms in cultured tobacco cells
    Sandra Matic
    Department of Cell and Organism Biology, Lund University, Sölvegatan 35B, 22362 Lund, Sweden
    Plant Physiol Biochem 42:299-306. 2004
    ..Possible roles of these two isoforms in the sucrose turnover in BY-2 cells are discussed...
  36. ncbi Intracellular glucose and binding of hexokinase and phosphofructokinase to particulate fractions increase under hypoxia in heart of the amazonian armored catfish (Liposarcus pardalis)
    Jason R Treberg
    Department of Biochemistry, Ocean Sciences Centre, Memorial University of Newfoundland, St John s, Newfoundland A1C 5S7, Canada
    Physiol Biochem Zool 80:542-50. 2007
    ..Such binding may be associated with increased glycolytic flux rates through modulation of enzyme-specific kinetics. The binding of HK and PFK occurs before any significant decrease in glycogen level...
  37. ncbi Glucose metabolism in the human preterm and term placenta of IUGR fetuses
    A L Magnusson
    Department of Physiology and Pharmacology, Perinatal Center, Goteborg University, Medicinaregatan 11, Box 432, S 405 30 Goteborg, Sweden
    Placenta 25:337-46. 2004
    ..These results do not support the hypothesis of increased placental glucose consumption but suggest an altered glycolytic pathway in the IUGR placenta...
  38. ncbi Emetine inhibits glycolysis in isolated, perfused rat hearts
    Shujia J Pan
    Department of Kinesiology, University of Texas at Austin, Austin, TX, USA
    Cardiovasc Toxicol 3:311-8. 2003
    ..Homogenate centrifugation studies indicate that emetine acts on bound rather than unbound phosphofructokinase. The inhibition may be uncompetitive in nature...
  39. ncbi Conservation of glycolytic oscillations in Saccharomyces cerevisiae and human pancreatic beta-cells: a study of metabolic robustness
    A S Silva
    Laboratorio de Biologia Molecular, Centro Infantil Boldrini, Campinas, SP, Brasil
    Genet Mol Res 5:525-35. 2006
    ....
  40. ncbi Increased muscle oxidative potential following resistance training induced fibre hypertrophy in young men
    Jason E Tang
    Department of Kinesiology, Exercise Metabolism Research Group, McMaster University, 1280 Main St W, Hamilton, ON L8S 4K1, Canada
    Appl Physiol Nutr Metab 31:495-501. 2006
    ..We conclude that resistance training provides a stimulus for improving muscle oxidative potential, as reflected by the increased activities of CS and beta-HAD following resistance training induced hypertrophy...
  41. ncbi Analysis of in vivo kinetics of glycolysis in aerobic Saccharomyces cerevisiae by application of glucose and ethanol pulses
    Diana Visser
    PURAC, P O Box 21, 4200 AA Gorinchem, The Netherlands
    Biotechnol Bioeng 88:157-67. 2004
    ..Our results confirm that FBP stimulates pyruvate kinase and suggest that this effect is pronounced. Furthermore, it appears that PEP does not play an important role in the allosteric regulation of phosphofructo kinase...
  42. ncbi Differing mechanisms of cold-induced changes in capillary supply in m. tibialis anterior of rats and hamsters
    Durmus Deveci
    Department of Physiology, Cumhuriyet University, 58140 Sivas, Turkey
    J Exp Biol 205:829-40. 2002
    ..These responses are consistent with the strategies of resisting and accommodating, respectively, an annual fall in environmental temperature...
  43. ncbi Different modes of activating phosphofructokinase, a key regulatory enzyme of glycolysis, in working vertebrate muscle
    G Wegener
    Institut fur Zoologie, Molekulare Physiologie, Johannes Gutenberg Universitat, Saarstrasse 21, D 55099 Mainz, Germany
    Biochem Soc Trans 30:264-70. 2002
    ..Whether this phenomenon exists in vertebrates other than the frog, and maybe even in humans, and how the content of F2,6P(2)in muscle is controlled are intriguing open questions...
  44. pmc Reconstructing the mosaic glycolytic pathway of the anaerobic eukaryote Monocercomonoides
    Natalia A Liapounova
    Department of Biological Sciences, The University of Calgary, Calgary, Alberta T2N 1N4, Canada
    Eukaryot Cell 5:2138-46. 2006
    ....
  45. ncbi Acute responses in muscle mitochondrial and cytosolic enzyme activities during heavy intermittent exercise
    H J Green
    Dept of Kinesiology, Univ of Waterloo, Waterloo, ON, Canada
    J Appl Physiol (1985) 104:931-7. 2008
    ....
  46. ncbi Suspected utility of enzymes with multiple activities in the small genome Mycoplasma species: the replacement of the missing "household" nucleoside diphosphate kinase gene and activity by glycolytic kinases
    J Dennis Pollack
    Department of Molecular Virology, Immunology, and Medical Genetics, The College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210, USA
    OMICS 6:247-58. 2002
    ..capricolum Kid(T) that their 6-phosphofructokinases (6-PFKs), phosphoglycerate kinases (PGKs), pyruvate kinases (PKs), and acetate kinases (AKs), besides ..
  47. ncbi Entamoeba histolytica: computer-assisted modeling of phosphofructokinase for the prediction of broad-spectrum antiparasitic agents
    C L Byington
    Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City 84132, USA
    Exp Parasitol 87:194-202. 1997
    ..The enzyme differs significantly from ATP-dependent phosphofructokinases found in humans and as such represents an important drug target...
  48. pmc Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus
    P Branny
    Laboratoire d Enzymologie du Centre National de la Recherche Scientifique, Gif sur Yvette, France
    J Bacteriol 175:5344-9. 1993
    ..The amino acid sequence of this enzyme was homologous to those of the ATP-dependent phosphofructokinases from E...
  49. pmc Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase
    Hideaki Tsuge
    Institute for Health Sciences, Tokushima Bunri University, Yamashiro cho, Japan
    Protein Sci 11:2456-63. 2002
    ..Those are ADP-dependent glucokinases (ADPGK) and phosphofructokinases in their glycolytic pathway. Here, we succeeded in gene cloning the ADPGK from P...
  50. pmc ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea
    C H Verhees
    Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen, The Netherlands
    J Bacteriol 183:7145-53. 2001
    ..Interestingly, uncharacterized homologs of this unusual kinase are present in several eucarya...
  51. pmc The structure of the ATP-bound state of S. cerevisiae phosphofructokinase determined by cryo-electron microscopy
    MONTSERRAT BARCENA
    University of Vermont, College of Medicine, Department of Molecular Physiology and Biophysics, Burlington, VT 05405, USA
    J Struct Biol 159:135-43. 2007
    ..Eukaryotic phosphofructokinases exhibit a far more sophisticated regulatory mechanism, they are more complex structures regulated by a ..
  52. ncbi Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosus
    J E Tuininga
    Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands
    J Biol Chem 274:21023-8. 1999
    ..the reverse reaction, nor was it regulated by any of the known allosteric modulators of ATP-dependent phosphofructokinases. ATP and AMP were identified as competitive inhibitors of the phosphofructokinase, raising the K(m) for ADP ..
  53. ncbi Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family member
    Rafael E Parducci
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Biochemistry 45:9291-9. 2006
    ..Inorganic phosphate, typically considered an activator of adenosine kinases, ribokinases, and phosphofructokinases (nonhomologous to Pfk-2) acted as an inhibitor of wild-type and E190Q mutant Pfk-2...
  54. pmc Structures of S. pombe phosphofructokinase in the F6P-bound and ATP-bound states
    Shaun Benjamin
    University of Vermont, College of Medicine, Department of Molecular Physiology and Biophysics, Burlington, VT 05405, USA
    J Struct Biol 159:498-506. 2007
    ..pombe subunit, the oligomeric organization of subunits in this enzyme is different from other yeast phosphofructokinases. Electron microscopy studies were carried out to reveal the quaternary structure of the homo-octameric Pfk1 ..
  55. ncbi A novel form of 6-phosphofructokinase. Identification and functional relevance of a third type of subunit in Pichia pastoris
    Katrin Tanneberger
    Institute of Biochemistry, Molecular Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
    J Biol Chem 282:23687-97. 2007
    Classically, 6-phosphofructokinases are homo- and hetero-oligomeric enzymes consisting of alpha subunits and alpha/beta subunits, respectively...
  56. ncbi Glucose catabolism of Escherichia coli strains with increased activity and altered regulation of key glycolytic enzymes
    M Emmerling
    Institute of Biotechnology, Eidgenossische Technische Hochschule Zurich, Switzerland
    Metab Eng 1:117-27. 1999
    ..Homologous and heterologous pyruvate kinases (Pyk) and phosphofructokinases (Pfk) were individually and simultaneously overexpressed. Overexpression of the E...
  57. ncbi 6-phosphofructokinase from Pichia pastoris: purification, kinetic and molecular characterization of the enzyme
    Jürgen Kirchberger
    Institut fur Biochemie, Medizinische Fakultät der Universität Leipzig, Liebigstrasse 16, D 04103 Leipzig, Germany
    Yeast 19:933-47. 2002
    ..structural organisation of the active enzyme seems to be different in comparison with hetero-octameric 6-phosphofructokinases from other yeast species...
  58. ncbi Purification and properties of the pyrophosphate-dependent phosphofructokinase from Dictyoglomus thermophilum Rt46 B.1
    Y H Ding
    Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand
    Extremophiles 3:131-7. 1999
    ..The enzyme was not allosterically controlled by traditional modulators of phosphofructokinases and has significant activity with tripolyphosphate and polyphosphate...
  59. ncbi Purification and characterization of an ADP-dependent phosphofructokinase from Thermococcus zilligii
    R S Ronimus
    Thermophile Research Unit, University of Waikato, Hamilton, New Zealand
    Extremophiles 3:121-9. 1999
    ..56mM (apparent Vmax of 2.9Umg(-1) and 12.5mM, respectively. The biochemical characteristics of this unique ADP-dependent enzymatic activity are compared to ATP and pyrophosphate-dependent phosphofructokinases.
  60. ncbi Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12
    Y Yang
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030 1501, USA
    FEMS Microbiol Lett 141:89-95. 1996
    ..acid sequence of PdxK has signature motifs of the PfkB superfamily of carbohydrate kinases, which includes phosphofructokinases and ribokinases, and suggests that three unidentified ORFs of Salmonella typhimurium, Haemophilus ..
  61. ncbi Diacylglyceride kinases, sphingosine kinases and NAD kinases: distant relatives of 6-phosphofructokinases
    Gilles Labesse
    Centre de Biochimie Structurale, INSERM U554 CNRS UMR5048, Universite Montpellier I, 15 Avenue Charles Flahault, 34060 Montpellier Cedex, France
    Trends Biochem Sci 27:273-5. 2002
    Diacylglyceride kinases, sphingosine kinases, NAD kinases and 6-phosphofructokinases are thought to be related despite large evolution of their sequences...
  62. ncbi Purification, microsequencing and cloning of spinach ATP-dependent phosphofructokinase link sequence and function for the plant enzyme
    Christian Winkler
    Institute of Botany III, University of Dusseldorf, Germany
    FEBS J 274:429-38. 2007
    ..that a large family of genomics-derived higher plant sequences currently annotated in the databases as putative pyrophosphate-dependent phosphofructokinases according to sequence similarity is misannotated with respect to the cosubstrate.
  63. pmc Mapping of glycolytic enzyme-binding sites on human erythrocyte band 3
    Haiyan Chu
    Chemistry Department, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA
    Biochem J 400:143-51. 2006
    ..Taken together, these results argue that band 3 provides a nucleation site for the GE complex on the human erythrocyte membrane and that other components near band 3 must also participate in organizing the enzyme complex...
  64. ncbi Expression of caveolin-1 in lymphocytes induces caveolae formation and recruitment of phosphofructokinase to the plasma membrane
    Johana Vallejo
    Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65212, USA
    FASEB J 19:586-7. 2005
    ..We therefore conclude that CAV-1 functions as a scaffolding protein for PFK and that this may contribute to the elucidation of the basis for carbohydrate compartmentation to the plasma membrane in a wide variety of cell types...
  65. ncbi Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism
    Nikolai Nordsborg
    Institute of Exercise and Sports Sciences, August Krogh Institute, 2100 Copenhagen, Denmark
    J Appl Physiol 95:1201-6. 2003
    ..In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished...
  66. pmc Hemolysis, myopathy, and cardiac disease associated with hereditary phosphofructokinase deficiency in two Whippets
    Karen Gerber
    Axiom Veterinary Laboratory, Newton Abbott, UK
    Vet Clin Pathol 38:46-51. 2009
    ..In addition to causing hemolysis and exertional myopathy, heart disease may be a prominent clinical component of PFK deficiency in this breed and has not been previously recognized in PFK-deficient English Springer Spaniels...
  67. ncbi NAD kinases use substrate-assisted catalysis for specific recognition of NAD
    Guillaume Poncet-Montange
    Atelier de Bio et Chimie Informatique Structurale, Centre de Biochimie Structurale, UMR 5048 CNRS, Universites Montpellier 1 et 2, 29 rue de Navacelles, Montpellier, France
    J Biol Chem 282:33925-34. 2007
    ..and directed mutagenesis have previously shown that NAD kinases (NADKs) and the distantly related 6-phosphofructokinases share the same catalytically important GGDGT motif...
  68. ncbi Diabetes causes marked changes in function and metabolism of rat neutrophils
    T C Alba-Loureiro
    Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Av Prof Lineu Prestes 1524, 05508 900 São Paulo SP, Brazil
    J Endocrinol 188:295-303. 2006
    ..The treatment with insulin abolished the changes induced by the diabetic state even with no marked change in glycemia. Therefore, insulin may have a direct effect on neutrophil metabolism and function...
  69. ncbi Drug effects on drug targets: inhibition of enzymes by neuroleptics, antimycotics, antibiotics and other drugs on human pathogenic amoebas and their anti-proliferative effects
    RAUL N ONDARZA
    Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico UNAM, University City, Mexico 04510, Mexico
    Recent Pat Antiinfect Drug Discov 2:206-16. 2007
    ....
  70. ncbi The first crystal structure of phosphofructokinase from a eukaryote: Trypanosoma brucei
    Jose Martinez-Oyanedel
    Structural Biochemistry Group, Institute of Structural and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh EH9 3JR, Scotland
    J Mol Biol 366:1185-98. 2007
    ....
  71. ncbi Mathematical simulation of membrane processes and metabolic fluxes of the pancreatic beta-cell
    Frank Diederichs
    Marschweg 10, D 29690 Schwarmstedt, Germany
    Bull Math Biol 68:1779-818. 2006
    ..The simulation shows that the so-called pyruvate paradox possibly results from a relatively low membrane conductance of beta-cells for pyruvate...
  72. ncbi Combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia
    Liviu Badea
    Bioinformatics Group, National Institute for Research in Informatics, Bucharest 011455, Romania
    Hepatogastroenterology 55:2016-27. 2008
    ..To address this problem, laser microdissection studies have been performed, but these have to deal with much smaller tissue sample quantities and therefore have significantly higher experimental noise...
  73. ncbi Regulation of the central glycolytic genes in Bacillus subtilis: binding of the repressor CggR to its single DNA target sequence is modulated by fructose-1,6-bisphosphate
    Thierry Doan
    Génétique Moléculaire et Cellulaire, INRA UMR216 CNRS URA1925 and INAP G, F 78850 Thiverval Grignon, France
    Mol Microbiol 47:1709-21. 2003
    ..Genomic sequence comparisons suggest that these results can apply to numerous low-G+C, Gram-positive bacterial species...
  74. ncbi Functional maps of the junctions between interglobular contacts and active sites in glycolytic enzymes -- a comparative analysis of the biochemical and structural data
    Ivan Y Torshin
    Laboratory of Kinetics and Catalysis, Chemical Department of Moscow State University, Moscow, Russia
    Med Sci Monit 8:BR123-35. 2002
    ..Since the conformation of an enzyme determines its catalytic properties, the structural mechanism(s) influencing the activity is of considerable interest...
  75. ncbi Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells
    Anna Karin Gustavsson
    Department of Physics, University of Gothenburg, Sweden
    FEBS J 281:2784-93. 2014
    ..The oscillatory manifold could be approximated by allosteric control values of phosphofructokinase for ATP and AMP...
  76. ncbi An in vivo approach to isolating allosteric pathways using hybrid multimeric proteins
    Cuijuan Tie
    Department of Biochemistry and Biophysics, Texas A and M University and Texas AgriLife Research, College Station, TX, USA
    Methods Mol Biol 796:307-15. 2012
    ..The in vivo method does increase the yield of the hybrids produced while decreasing the time required for their isolation...
  77. ncbi Ventricle-specific metabolic differences in the newborn piglet myocardium in vivo and during arrested global ischemia
    Danny Quaglietta
    Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Pediatr Res 63:15-9. 2008
    ..This profile may offer one explanation for greater LV-dysfunction relative to the RV in children following ischemia...
  78. ncbi The recovery of dipolar relaxation times from fluorescence decays as a tool to probe local dynamics in single tryptophan proteins
    Giampiero Mei
    Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome 00133, Italy
    Arch Biochem Biophys 417:159-64. 2003
    ..These findings may have important implications on the analysis of small-scale protein dynamics, since dielectric relaxation directly probes a local structural change around the excited state of tryptophan...
  79. pmc Expression of phosphofructokinase in Neisseria meningitidis
    Gino J E Baart
    Netherlands Vaccine Institute NVI, Unit Research and Development, PO Box 457, 3720 AL Bilthoven, The Netherlands
    Microbiology 156:530-42. 2010
    ....
  80. ncbi Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophila
    R Ronimus
    Department of Biological Sciences, University of Waikato, Hamilton, New Zealand
    Arch Microbiol 175:308-12. 2001
    ..Phylogenetic analysis demonstrated a close relationship between the thermophilic S. thermophila phosphofructokinase and the large beta-subunits of the phosphofructokinases from Borrelia burgdorferi and Treponema pallidum.
  81. pmc The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases
    Colin P Kenyon
    CSIR, Biosciences, Meiring Naude Road, Pretoria, 0001, Gauteng, South Africa
    BMC Biochem 12:36. 2011
    ..4 μM to in excess of 1000 μM for ATP. It is not known how this diverse range of enzymes mechanistically achieves the regulation of catalysis via an affinity range for ATP varying by three-orders of magnitude...
  82. ncbi From genome to enzyme: analysis of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium Synechocystis sp. PCC 6803
    Vicki L Knowles
    Department of Biology, Queen s University, Kingston, Ontario, K7L 3N6 Canada
    Plant Cell Physiol 44:758-63. 2003
    ..Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated)...
  83. pmc Redefining the role of the quaternary shift in Bacillus stearothermophilus phosphofructokinase
    Rockann Mosser
    Department of Biochemistry and Biophysics, Texas A and M University, and Texas A and M AgriLife Research, College Station, TX 77843 2128, USA
    Biochemistry 52:5421-9. 2013
    ..The results suggest that the main role of the quaternary shift may be to influence ligand binding and not to cause the heterotropic allosteric inhibition per se. ..
  84. ncbi Control of glycolysis in mature boar spermatozoa: effect of pH in vitro
    A R Jones
    School of Molecular and Microbial Biosciences, Discipline of Biochemistry, The University of Sydney, Sydney, NSW, Australia
    Reprod Fertil Dev 16:319-24. 2004
    ..Phosphofructokinase has been shown to be sensitive to this drop in pH rather than to the build up of lactate ions or ATP, thereby controlling the rate of glycolysis in vitro...
  85. pmc Quantification of allosteric influence of Escherichia coli phosphofructokinase by frequency domain fluorescence
    Audrey S Pham
    Division of Pathology and Laboratory Medicine, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Biophys J 85:656-66. 2003
    ..Coupling interactions giving rise to positive entropy components are manifested by increasing flexibility of the ternary complexes rather than the binary complexes...
  86. pmc A broad specificity nucleoside kinase from Thermoplasma acidophilum
    Sarah R Elkin
    Department of Chemistry, University of Virginia, Charlottesville, VA 22904 4319, USA
    Proteins 81:568-82. 2013
    ..PfkB family of carbohydrate kinases (a family member of the Ribokinase clan) which include ribokinases, 1-phosphofructokinases, 6-phosphofructo-2-kinase, inosine/guanosine kinases, fructokinases, adenosine kinases, and many more...
  87. ncbi A metronome-like control of the calcium signal leading to nuclear envelope breakdown and mitosis in sand dollar (Echinaracnius parma) cells
    Robert B Silver
    Department of Pharmacology, School of Medicine, Wayne State University, Detroit, Michigan, USA
    Biol Bull 203:213-5. 2002
  88. ncbi Wavenumber distribution in Hopf-wave instability: the reversible Selkov model of glycolytic oscillation
    Arun K Dutt
    Faculty of Computing, Engineering and Mathematical Sciences, Du Pont Building, University of the West of England, Frenchay Campus, Bristol BS16 1QY, United Kingdom
    J Phys Chem B 109:17679-82. 2005
    ....
  89. ncbi Expression, purification, crystallization and preliminary crystallographic analysis of Trypanosoma brucei phosphofructokinase
    Jeffrey W Keillor
    Departement de Chimie, Universite de Montreal, CP 6128, Succursale Centre Ville, Montreal, Québec H3C 1J7, Canada
    Acta Crystallogr D Biol Crystallogr 59:532-4. 2003
    ..The availability of this first eukaryotic PFK structure will be of particular significance for structure-based drug design and will also provide important additional structural evidence for the allosteric control of PFK activity...
  90. pmc Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast
    Guillermo G Zampar
    Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands
    Mol Syst Biol 9:651. 2013
    ..Taken together, our results represent an important contribution toward a systems-level understanding of how this adaptation is realized...
  91. ncbi Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus
    L M Orchard
    Department of Biochemistry, University of Cambridge, U K
    Proc Biol Sci 242:87-90. 1990
    ..coli (pfkA) and the 6-phosphofructokinases present in a number of pro- and eukaryotic organisms, but there is 27%, 25% and 22% identify of sequence ..
  92. ncbi The HIF-1 response to simulated ischemia in mouse skeletal muscle cells neither enhances glycolysis nor prevents myotube cell death
    Nathalie Dehne
    Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstr 55, D 45122 Essen, Germany
    Am J Physiol Regul Integr Comp Physiol 293:R1693-701. 2007
    ..In contrast, myoblasts survive ischemia and thus may play an important role during regeneration and HIF-1-induced revascularization...
  93. ncbi Effect of ethylene treatment on the concentration of fructose-2,6-bisphosphate and on the activity of phosphofructokinase 2/fructose-2,6-bisphosphatase in banana
    E Mertens
    Laboratoire de Chimie Physiologique, Universite Catholique de Louvain, Bruxelles, Belgium
    Eur J Biochem 167:579-83. 1987
    ..enzyme, but also by an early increase in the concentration of fructose 6-phosphate, the substrate of all phosphofructokinases, and also by the decrease in the concentration of glycerate 3-phosphate, a potent inhibitor of ..
  94. ncbi Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
    Yipeng Wang
    Department of Biochemistry and Cell Biology, MS 140, Rice University, 6100 Main Street, Houston, TX 77005 1892, USA
    Appl Microbiol Biotechnol 97:6883-93. 2013
    ..The results indicated that the flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis. ..
  95. ncbi An essential methionine residue involved in substrate binding by phosphofructokinases
    X Wang
    Department of Microbiology, Chicago Medical School, Illinois 60064, USA
    Biochem Biophys Res Commun 250:466-8. 1998
    An alignment of all PPi-dependent phosphofructokinases and all allosteric ATP-dependent PFKs shows relatively few residues that are fully conserved...
  96. ncbi Differential gene expression in neutrophils from patients with generalized aggressive periodontitis
    T Kubota
    Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata, Japan
    J Periodontal Res 36:390-7. 2001
    ..Neutrophil functions in Aggressive-P patients are suggested to be altered by regulatory factors of the immune system including HSF4b (transcription factor), Zf9 (activator of TGF-beta) and muskelin (cellular adhesion)...
  97. pmc Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans
    Martin Thomassen
    Department of Nutrition, Exercise and Sports, Section of Integrated Physiology, University of Copenhagen, August Krogh Building, Universitetsparken 13, 2 sal, 2100 Copenhagen, Denmark
    J Physiol 591:1523-33. 2013
    ..This, together with the observation of a higher abundance of the Na(+)-K(+) pump α2 isoform protein in type II fibres, is likely to have importance for the exercise-induced human Na(+)-K(+) pump activity in the different fibre types...
  98. ncbi Neuroprotection conferred by post-ischemia ethanol therapy in experimental stroke: an inhibitory effect on hyperglycolysis and NADPH oxidase activation
    Ryan Kochanski
    Department of Neurosurgery, Wayne State University School of Medicine, Detroit, Michigan, USA
    J Neurochem 126:113-21. 2013
    ..Because of the lack of associated hypoglycemia and selectivity toward decreasing cerebral metabolism, further investigation of ethanol's use as a post-stroke therapy, especially in the context of hyperglycemia, seems warranted...
  99. pmc Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits
    Angelo Scuteri
    Unità Operativa Geriatria, Istituto per la Patologia Endocrina e Metabolica, Rome, Italy
    PLoS Genet 3:e115. 2007
    ..These changes could have a significant impact on the risk of obesity-related morbidity in the general population...
  100. ncbi Genetic polymorphism in Dirofilaria immitis
    Catherine Bourguinat
    Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte Anne de Bellevue, H9X3V9 Quebec, Canada
    Vet Parasitol 176:368-73. 2011
    ..Differences in F coefficients were observed between the groups. D. immitis was found to be genetically heterogeneous. This genetic heterogeneity has implication for the development of genetically selected strains of the parasite...
  101. pmc Metabolic and transcriptomic adaptation of Lactococcus lactis subsp. lactis Biovar diacetylactis in response to autoacidification and temperature downshift in skim milk
    Sandy Raynaud
    Laboratoire Biotechnologie Bioprocédés, UMR 5504 INSA CNRS and UMR 792 INSA INRA, Institut National des Sciences Appliquees, 135 Avenue de Rangueil, 31077 Toulouse Cedex 4, France
    Appl Environ Microbiol 71:8016-23. 2005
    ..If specific responses to acid and cold stresses were identified, this exhaustive analysis also enabled induction of unexpected pathways to be shown...

Research Grants1

  1. STRUCTURE AND EXPRESSION OF THE PARAMYXOVIRUS SV5 GENOME
    Robert A Lamb; Fiscal Year: 2010
    ..We will elucidate the role of mutations in the F protein in the context of a virus infection by using reverse genetics and we will study fusion activity, virus growth and virus assembly of the altered viruses. ..