rna helicases

Summary

Summary: A family of proteins that promote unwinding of RNA during splicing and translation. EC 2.7.7.-.

Top Publications

  1. ncbi IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency
    Nat Immunol 6:981-8. 2005
  2. pmc SF1 and SF2 helicases: family matters
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 20:313-24. 2010
  3. ncbi Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses
    Hiroki Kato
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Nature 441:101-5. 2006
  4. pmc Yeast Sen1 helicase protects the genome from transcription-associated instability
    Hannah E Mischo
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 41:21-32. 2011
  5. pmc RNA helicases at work: binding and rearranging
    Eckhard Jankowsky
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Trends Biochem Sci 36:19-29. 2011
  6. doi Translocation and unwinding mechanisms of RNA and DNA helicases
    Anna Marie Pyle
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute and Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Biophys 37:317-36. 2008
  7. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
  8. ncbi Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity
    Mitsutoshi Yoneyama
    Antiviral Innate Immunity Project, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Tokyo, Japan
    J Immunol 175:2851-8. 2005
  9. pmc P body-associated protein Mov10 inhibits HIV-1 replication at multiple stages
    Ryan Burdick
    HIV Drug Resistance Program, National Cancer Institute Frederick, Viral Mutation Section, Frederick, MD 21702, USA
    J Virol 84:10241-53. 2010
  10. pmc An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila
    Daniel Olivieri
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Vienna, Austria
    EMBO J 29:3301-17. 2010

Research Grants

  1. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2007
  2. Structure and Function of the Hepatitis C Virus Genome
    PETER contact SARNOW; Fiscal Year: 2010
  3. Antiviral potential of helicase inhibitors
    David N Frick; Fiscal Year: 2010
  4. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2010
  5. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 1993
  6. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2007
  7. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
  8. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 2003
  9. MOLECULAR BIOLOGY OF POLIOVIRUS RNA REPLICATION
    JAMES FLANEGAN; Fiscal Year: 2003
  10. Protein Arginine Methylation in Trypanosomes
    Laurie K Read; Fiscal Year: 2010

Detail Information

Publications335 found, 100 shown here

  1. ncbi IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency
    Nat Immunol 6:981-8. 2005
    ..CARD-like structure that mediated interaction with the CARD of RIG-I and Mda5, which are cytoplasmic RNA helicases that sense viral infection...
  2. pmc SF1 and SF2 helicases: family matters
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 20:313-24. 2010
    ..We discuss recent data in the context of this unifying view of SF1 and SF2 helicases...
  3. ncbi Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses
    Hiroki Kato
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Nature 441:101-5. 2006
    ..Together, our data show that RIG-I and MDA5 distinguish different RNA viruses and are critical for host antiviral responses...
  4. pmc Yeast Sen1 helicase protects the genome from transcription-associated instability
    Hannah E Mischo
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 41:21-32. 2011
    ..Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability...
  5. pmc RNA helicases at work: binding and rearranging
    Eckhard Jankowsky
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Trends Biochem Sci 36:19-29. 2011
    b>RNA helicases are ubiquitous, highly conserved enzymes that participate in nearly all aspects of RNA metabolism. These proteins bind or remodel RNA or RNA-protein complexes in an ATP-dependent fashion...
  6. doi Translocation and unwinding mechanisms of RNA and DNA helicases
    Anna Marie Pyle
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute and Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Biophys 37:317-36. 2008
    ..Through structural and single-molecule investigations, researchers are developing coherent models to explain the functions and dynamic motions of helicase enzymes...
  7. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
    ..Subsequent gene activation by these factors induced antiviral functions, including type I interferon production. Thus, RIG-I is key in the detection and subsequent eradication of the replicating viral genomes...
  8. ncbi Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity
    Mitsutoshi Yoneyama
    Antiviral Innate Immunity Project, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Tokyo, Japan
    J Immunol 175:2851-8. 2005
    ..These results highlight ingenious mechanisms for initiating antiviral innate immune responses and the action of virus-encoded inhibitors...
  9. pmc P body-associated protein Mov10 inhibits HIV-1 replication at multiple stages
    Ryan Burdick
    HIV Drug Resistance Program, National Cancer Institute Frederick, Viral Mutation Section, Frederick, MD 21702, USA
    J Virol 84:10241-53. 2010
    ..Overall, these results show that Mov10 can potently inhibit HIV-1 replication at multiple stages...
  10. pmc An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila
    Daniel Olivieri
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Vienna, Austria
    EMBO J 29:3301-17. 2010
    ..Loss of Zucchini leads to an accumulation of Piwi and Armitage in Yb bodies, indicating that Yb bodies are sites of primary piRNA biogenesis...
  11. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
  12. ncbi Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
    ..within infected cells and is sensed by Toll-like receptor 3 (TLR3) and the recently identified cytosolic RNA helicases RIG-I (retinoic acid inducible gene I, also known as Ddx58) and Mda5 (melanoma differentiation-associated gene ..
  13. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage...
  14. pmc The human VASA gene is specifically expressed in the germ cell lineage
    D H Castrillon
    Women s and Perinatal Pathology Division, Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:9585-90. 2000
    ..The gene we have isolated is thus a highly specific marker of germ cells and should be useful for studies of human germ cell determination and function...
  15. pmc Perturbation of the P-body component Mov10 inhibits HIV-1 infectivity
    Vyacheslav Furtak
    Department of Microbiology, New York University School of Medicine, New York, New York, United States of America
    PLoS ONE 5:e9081. 2010
    ..Because Mov10 is implicated as part of the P-body complex, these findings point to the potential role of cytoplasmic RNA processing machinery in infectious retroviral production...
  16. pmc DEAD-box proteins as RNA helicases and chaperones
    Inga Jarmoskaite
    Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA
    Wiley Interdiscip Rev RNA 2:135-52. 2011
    ..This review will describe the properties of DEAD-box proteins as RNA helicases and the current understanding of how the energy from ATPase activity is used to drive the separation of RNA ..
  17. pmc Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway
    Ke Zheng
    Center for Animal Transgenesis and Germ Cell Research and Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 107:11841-6. 2010
    ..The Mov10l1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins...
  18. pmc LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses
    Takashi Satoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:1512-7. 2010
    ..Taken together, the present data suggest that LGP2 facilitates viral RNA recognition by RIG-I and MDA5 through its ATPase domain...
  19. pmc Moloney leukemia virus 10 (MOV10) protein inhibits retrovirus replication
    Xiaojun Wang
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    J Biol Chem 285:14346-55. 2010
    ..All of these results indicate that MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection...
  20. ncbi UPF1 is required for nonsense-mediated mRNA decay (NMD) and RNAi in Arabidopsis
    Luis Arciga-Reyes
    Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK
    Plant J 47:480-9. 2006
    ..Finally, gene silencing by an inverted repeat transgene is impaired in upf1-5 mutants, indicating a connection between UPF1 and RNA interference in plants...
  21. pmc Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila
    Kuniaki Saito
    Keio University School of Medicine, Tokyo, Japan
    Genes Dev 24:2493-8. 2010
    ..Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing...
  22. pmc MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs
    Robert J A Frost
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 107:11847-52. 2010
    ....
  23. ncbi Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities
    L Liang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Development 120:1201-11. 1994
    ..Initial recruitment of vasa to the pole plasm must consequently depend upon protein-protein interactions but, once localized, vasa must bind to RNA to mediate germ cell formation...
  24. ncbi RNA chaperones, RNA annealers and RNA helicases
    Lukas Rajkowitsch
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    RNA Biol 4:118-30. 2007
    ..DExD/H-box containing RNA helicases are discussed as a special family of enzymes that restructure RNA or RNPs in an ATP-dependent manner...
  25. pmc RNA helicases: emerging roles in viral replication and the host innate response
    Arnaz Ranji
    Department of Veterinary Biosciences, Ohio State University, Columbus, OH, USA
    RNA Biol 7:775-87. 2010
    b>RNA helicases serve multiple roles at the virus-host interface...
  26. ncbi The BRCA1-interacting helicase BRIP1 is deficient in Fanconi anemia
    Orna Levran
    Laboratory for Human Genetics and Hematology, The Rockefeller University, New York, New York, USA
    Nat Genet 37:931-3. 2005
    ..Using genetic mapping, mutation identification and western-blot data, we identify the defective protein in FA-J cells as BRIP1 (also called BACH1), a DNA helicase that is a binding partner of the breast cancer tumor suppressor BRCA1...
  27. pmc Endogenous MOV10 inhibits the retrotransposition of endogenous retroelements but not the replication of exogenous retroviruses
    Shetal Arjan-Odedra
    Department of Infectious Diseases, King s College London School of Medicine, Borough Wing, Guy s Hospital, London Bridge, London, SE1 9RT, UK
    Retrovirology 9:53. 2012
    ..Here, we employ both ectopic expression and gene knockdown approaches to analyse the role of human MOV10 in the replication of a panel of exogenous retroviruses and endogenous retroelements...
  28. doi Ataxia with oculomotor apraxia type 2: clinical, biological and genotype/phenotype correlation study of a cohort of 90 patients
    M Anheim
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, Universite de Strasbourg, INSERM, Illkirch, France
    Brain 132:2688-98. 2009
    ..001, P = 0.008 and P = 0.01, respectively). The lack of pyramidal signs in most patients may be explained by masking due to severe motor neuropathy...
  29. doi Evolutional conservation of molecular structure and antiviral function of a viral RNA receptor, LGP2, in Japanese flounder, Paralichthys olivaceus
    Maki Ohtani
    Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, South Korea
    J Immunol 185:7507-17. 2010
    ..These results suggest that Japanese flounder LGP2 plays an important role in the recognition of both viral ssRNA and dsRNA to induce the antiviral activity by the production of IFN-stimulated proteins...
  30. doi mRNA helicases: the tacticians of translational control
    Armen Parsyan
    Department of Biochemistry and Goodman Cancer Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec H3A 1A3, Canada
    Nat Rev Mol Cell Biol 12:235-45. 2011
    ....
  31. pmc Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila
    Astrid D Haase
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Genes Dev 24:2499-504. 2010
    ..Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis...
  32. pmc The hepatitis C virus NS3 protein: a model RNA helicase and potential drug target
    David N Frick
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Curr Issues Mol Biol 9:1-20. 2007
    ....
  33. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
    ....
  34. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  35. pmc Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I
    Rhea Sumpter
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    J Virol 79:2689-99. 2005
    ..RIG-I is thus a pathogen receptor that regulates cellular permissiveness to HCV replication and, as an interferon-responsive gene, may play a key role in interferon-based therapies for the treatment of HCV infection...
  36. ncbi RISC assembly defects in the Drosophila RNAi mutant armitage
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 116:831-41. 2004
    ..Our results suggest that armi is required for RISC maturation...
  37. pmc Regulation of alternative splicing by the ATP-dependent DEAD-box RNA helicase p72
    Arnd Honig
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:5698-707. 2002
    Although a number of ATP-dependent RNA helicases are important for constitutive RNA splicing, no helicases have been implicated in alternative RNA splicing...
  38. ncbi Further characterization of the helicase activity of eIF4A. Substrate specificity
    G W Rogers
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4935, USA
    J Biol Chem 276:12598-608. 2001
    Eukaryotic initiation factor (eIF) 4A is the archetypal member of the DEAD box family of RNA helicases and is proposed to unwind structures in the 5'-untranslated region of mRNA to facilitate binding of the 40 S ribosomal subunit...
  39. doi RNA helicases p68 and p72: multifunctional proteins with important implications for cancer development
    Frances V Fuller-Pace
    Centre for Oncology and Molecular Medicine, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK
    Future Oncol 7:239-51. 2011
    The DEAD box RNA helicases p68 (DDX5) and p72 (DDX17) play important roles in multiple cellular processes that are commonly dysregulated in cancers, including transcription, pre-mRNA processing/alternative splicing and miRNA processing...
  40. ncbi RNA structural rearrangement via unwinding and annealing by the cyanobacterial RNA helicase, CrhR
    Danuta Chamot
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    J Biol Chem 280:2036-44. 2005
    Rearrangement of RNA secondary structure is crucial for numerous biological processes. RNA helicases participate in these rearrangements through the unwinding of duplex RNA...
  41. pmc The Yb body, a major site for Piwi-associated RNA biogenesis and a gateway for Piwi expression and transport to the nucleus in somatic cells
    Hongying Qi
    Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511, USA
    J Biol Chem 286:3789-97. 2011
    ..Finally, we discovered putative endo-siRNAs in the flamenco locus and the Yb dependence of their expression. These observations further implicate a role for Yb in transposon silencing via both the piRNA and endo-siRNA pathways...
  42. ncbi VISA is an adapter protein required for virus-triggered IFN-beta signaling
    Liang Guo Xu
    Department of Immunology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
    Mol Cell 19:727-40. 2005
    ..These findings suggest that VISA is critically involved in both virus-triggered TLR3-independent and TLR3-mediated antiviral IFN signaling...
  43. pmc Mechanism and specificity of a symmetrical benzimidazolephenylcarboxamide helicase inhibitor
    Craig A Belon
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Biochemistry 49:1822-32. 2010
    ..Results are discussed in light of a molecular model of a (BIP)(2)B-HCV helicase complex, which is unable to bind nucleic acid, thus preventing the enzyme from separating double-stranded nucleic acid...
  44. ncbi The serine protease domain of hepatitis C viral NS3 activates RNA helicase activity by promoting the binding of RNA substrate
    Rudolf K F Beran
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 282:34913-20. 2007
    ....
  45. pmc Sen1p performs two genetically separable functions in transcription and processing of U5 small nuclear RNA in Saccharomyces cerevisiae
    Jonathan S Finkel
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 184:107-18. 2010
    ..The results suggest that the Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression...
  46. pmc Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2
    Darja Bamming
    Department of Biochemistry, Molecular Biology, and Cell Biology and Department of Medicine, Northwestern University, USA
    J Biol Chem 284:9700-12. 2009
    ..components of the cellular response to virus infection and are homologous to the DEXH box subfamily of RNA helicases. However, the relevance of helicase activity in the regulation of interferon production remains elusive...
  47. pmc deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2
    W M Toone
    Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada
    J Bacteriol 173:3291-302. 1991
    ..We suggest that the DeaD protein plays a hitherto unknown role in translation in E. coli...
  48. pmc Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction
    Kay Childs
    Division of Basic Medical Sciences, St George s, University of London, London, United Kingdom
    J Virol 86:3411-21. 2012
    ..This is significant, because it demonstrates a general mechanism for the targeting of the RIG-I pathway by paramyxoviruses...
  49. pmc Identification of molecular determinants from Moloney leukemia virus 10 homolog (MOV10) protein for virion packaging and anti-HIV-1 activity
    Aierken Abudu
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    J Biol Chem 287:1220-8. 2012
    ..Taken together, we have identified two critical MOV10 packaging determinants and eight other critical residues for anti-HIV-1 activity. These results provide a molecular basis for further understanding the MOV10 antiretroviral mechanism...
  50. ncbi Innate immune recognition of viral infection
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Suita, Osaka 565 0871, Japan
    Nat Immunol 7:131-7. 2006
    ..Cells also express cytoplasmic RNA helicases that function as alternative pattern-recognition receptors through recognition of double-stranded RNA produced ..
  51. ncbi The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis
    N Kyle Tanner
    Departement de Biochimie Medicale, Centre Medical Universitaire, 1, rue Michel Servet, 1211 Geneva 4, Switzerland
    Mol Cell 11:127-38. 2003
    ..We have discovered a motif upstream of motif I that is unique to and characteristic of the DEAD box family of RNA helicases. It consists of a 9 amino acid sequence containing an invariant glutamine...
  52. ncbi Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Mol Cell 22:561-9. 2006
    ..Recently, a second system has been described, which relies on the cytoplasmic recognition of dsRNA by RNA helicases such as RIG-I...
  53. pmc The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:795-804. 2008
    ..Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3...
  54. ncbi The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification
    Heather A Cook
    Program in Molecular Medicine and the Program in Cell Dynamics, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Cell 116:817-29. 2004
    ..We conclude that RNA silencing is essential for establishment of the cytoskeletal polarity that initiates embryonic axis specification and for translational control of oskar mRNA...
  55. ncbi Single processing center models for human Dicer and bacterial RNase III
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 118:57-68. 2004
    ..Based on these and other data, we propose that Dicer functions through intramolecular dimerization of its two RNase III domains, assisted by the flanking RNA binding domains, PAZ and dsRBD...
  56. ncbi The ATPase, RNA unwinding, and RNA binding activities of recombinant p68 RNA helicase
    Youliang Huang
    Program in Cell and Molecular Biosciences, Department of Animal and Dairy Sciences, Auburn University, 210 Upchurch Hall, Auburn, Alabama 36849, USA
    J Biol Chem 277:12810-5. 2002
    ..Interestingly, the recombinant p68 RNA helicase unwinds dsRNA in both 3' --> 5' and 5' --> 3' directions. This is the second example of a Asp-Glu-Ala-Asp (DEAD) box RNA helicase that unwinds RNA duplexes in a bi-directional manner...
  57. pmc An RNA degradosome assembly in Caulobacter crescentus
    Steven W Hardwick
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    Nucleic Acids Res 39:1449-59. 2011
    ..Finally, we observe that the abundance of RNase E varies through the cell cycle, with maxima at morphological differentiation and cell division. This variation may contribute to the program of gene expression during cell division...
  58. ncbi Truncating mutations in the Fanconi anemia J gene BRIP1 are low-penetrance breast cancer susceptibility alleles
    Sheila Seal
    Section of Cancer Genetics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
    Nat Genet 38:1239-41. 2006
    ..Thus, inactivating truncating mutations of BRIP1, similar to those in BRCA2, cause Fanconi anemia in biallelic carriers and confer susceptibility to breast cancer in monoallelic carriers...
  59. ncbi Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases
    Thierry Bizebard
    Laboratoire de Biochimie et Enzymologie Structurales, CNRS UPR 9063, Bat 34, CNRS, 91198 Gif sur Yvette, Cedex, France
    Biochemistry 43:7857-66. 2004
    ..This behavior, which contrasts with that of well studied SF1 DNA helicases, is discussed in the light of available structural and biochemical data...
  60. ncbi vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development
    S Styhler
    Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, Canada
    Development 125:1569-78. 1998
    ..However, GRK accumulation in the oocyte is severely reduced in the absence of vasa function, suggesting a function for VASA in activating gurken translation in wild-type ovaries...
  61. ncbi Dynamics and processivity of 40S ribosome scanning on mRNA in yeast
    Karine Berthelot
    Department of Biomolecular Sciences, UMIST, Manchester, UK
    Mol Microbiol 51:987-1001. 2004
    ..We have developed a biased bidirectional walking model of ribosomal scanning that provides a framework for understanding the above observations as well as other known quantitative and qualitative features of this process...
  62. ncbi ATP- and ADP-dependent modulation of RNA unwinding and strand annealing activities by the DEAD-box protein DED1
    Quansheng Yang
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Biochemistry 44:13591-601. 2005
    DEAD-box RNA helicases, which are involved in virtually all aspects of RNA metabolism, are generally viewed as enzymes that unwind RNA duplexes or disrupt RNA-protein interactions in an ATP-dependent manner...
  63. pmc Mechanism for Aar2p function as a U5 snRNP assembly factor
    Gert Weber
    Fachbereich Biologie Chemie Pharmazie, Abteilung Strukturbiochemie, Freie Universitat Berlin, D 14195 Berlin, Germany
    Genes Dev 25:1601-12. 2011
    ..The purpose of this regulation may be to safeguard against nonspecific RNA binding to Prp8p and/or premature activation of Brr2p activity...
  64. pmc The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter
    J Andrejeva
    School of Biology, Biomolecular Sciences Building, North Haugh, University of St Andrews, Fife KY16 9TS, United Kingdom
    Proc Natl Acad Sci U S A 101:17264-9. 2004
    ....
  65. ncbi Structural domains involved in the RNA folding activity of RNA helicase II/Gu protein
    B C Valdez
    Department of Pharmacology, Baylor College of Medicine, Houston, USA
    Eur J Biochem 267:6395-402. 2000
    ..The last 17 amino acids of RH II/Gu can be replaced by an RNA binding motif from nucleolar protein p120 without a deleterious effect on its foldase activity. A model is proposed to explain how RH II/Gu binds and folds an RNA substrate...
  66. pmc A single positively selected West Nile viral mutation confers increased virogenesis in American crows
    Aaron C Brault
    Center for Vector Borne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California 95616, USA
    Nat Genet 39:1162-6. 2007
    ..These phenotypic and evolutionary results provide compelling evidence for the positive selection of a mutation encoding increased viremia potential and virulence in the AMCR sentinel bird species...
  67. pmc Transcriptome-wide binding sites for components of the Saccharomyces cerevisiae non-poly(A) termination pathway: Nrd1, Nab3, and Sen1
    Tyler J Creamer
    Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    PLoS Genet 7:e1002329. 2011
    ..In addition, we show that Sen1 does not cross-link efficiently to many expected non-coding RNAs but does cross-link to the 3' end of most pre-mRNA transcripts, suggesting an extensive role in mRNA 3' end formation and/or termination...
  68. pmc Both introns and long 3'-UTRs operate as cis-acting elements to trigger nonsense-mediated decay in plants
    Sándor Kertész
    Agricultural Biotechnology Center, Godollo, Hungary
    Nucleic Acids Res 34:6147-57. 2006
    ..We have also identified plant UPF1 and showed that tethering of UPF1 to either the 5'- or 3'-UTR of an mRNA results in reduced transcript accumulation. Thus, plant UPF1 might bind to mRNA in a late, irreversible phase of NMD...
  69. doi Synthesis of new acridone derivatives, inhibitors of NS3 helicase, which efficiently and specifically inhibit subgenomic HCV replication
    Anna Stankiewicz-Drogon
    Institute of Biochemistry and Biophysics, PAS, Pawinskiego 5A, 02 106 Warsaw, Poland
    J Med Chem 53:3117-26. 2010
    ..Although the mechanism of action of 16 in the replicon system remains unclear, it is the key lead compound for further development of anti-HCV drugs...
  70. ncbi Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA
    Indira Umareddy
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos Building, Singapore
    J Gen Virol 87:2605-14. 2006
    ..These results suggest that NS4B modulates dengue virus replication via its interaction with NS3...
  71. ncbi Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes
    Kui Li
    Department of Microbiology and Immunology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas 77555 1019, USA
    J Biol Chem 280:16739-47. 2005
    ..We conclude that hepatocytes contain two distinct antiviral signaling pathways leading to expression of type I IFNs, one dependent upon TLR3 and the other dependent on RIG-I, with little cross-talk between these pathways...
  72. doi Identification and functional analysis of RNase E of Vibrio angustum S14 and two-hybrid analysis of its interaction partners
    Melissa A Erce
    Systems Biology Laboratory, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
    Biochim Biophys Acta 1794:1107-14. 2009
    ..The role of RNase E as a hub protein and the implications of microdomain-mediated interactions in relation to specificity and function are discussed...
  73. pmc Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila
    Joao Trindade Marques
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois, USA
    Nat Struct Mol Biol 17:24-30. 2010
    ..Loqs is primarily required for dsRNA processing, whereas R2D2 is essential for the subsequent loading of siRNAs into effector Ago-RISC complexes...
  74. doi Quantitative expression of RIG-like helicase, NOD-like receptor and inflammasome-related mRNAs in humans and mice
    Maciej Lech
    Medizinische Poliklinik, University of Munich, 80336 Munich, Germany
    Int Immunol 22:717-28. 2010
    ..These data suggest that the cell-type-, organ- and species-specific expression and regulation need to be considered in the design and interpretation of related studies...
  75. pmc The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
    Katsutomo Okamura
    Sloan Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Avenue, Box 252, New York, New York 10065, USA
    Nature 453:803-6. 2008
    ..These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila...
  76. pmc Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein
    Dahai Luo
    Structural and Computational Biology Division, School of Biological Sciences, Nanyang Technological University, Singapore
    EMBO J 27:3209-19. 2008
    ..They also suggest how NS3 could exert an effect as an RNA-anchoring device and thus participate both in flavivirus RNA replication and assembly...
  77. pmc The requirement for eukaryotic initiation factor 4A (elF4A) in translation is in direct proportion to the degree of mRNA 5' secondary structure
    Y V Svitkin
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada
    RNA 7:382-94. 2001
    ..elF4A possesses ATPase and RNA helicase activities and is the prototype for a large family of putative RNA helicases (the DEAD box family)...
  78. ncbi Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells
    C Yoon
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    Development 124:3157-65. 1997
    ..Furthermore, they provide the basis for further studies on this novel RNA localization pattern and on germ-line development in general...
  79. pmc mRNAs encoding telomerase components and regulators are controlled by UPF genes in Saccharomyces cerevisiae
    Jeffrey N Dahlseid
    Department of Chemistry, St Olaf College, Northfield, Minnesota 55057, USA
    Eukaryot Cell 2:134-42. 2003
    ..Together, these results suggest that NMD maintains the balance of gene products that control telomere length and telomeric silencing primarily by maintaining appropriate levels of STN1, TEN1, and EST2 mRNA...
  80. pmc Senataxin, defective in ataxia oculomotor apraxia type 2, is involved in the defense against oxidative DNA damage
    Amila Suraweera
    Radiation Biology and Oncology Laboratory, Queensland Institute of Medical Research, Brisbane, QLD 4029, Australia
    J Cell Biol 177:969-79. 2007
    ..These results provide evidence that an additional member of the autosomal recessive AOA is also characterized by a defective response to DNA damage, which may contribute to the neurodegeneration seen in this syndrome...
  81. ncbi Modulation of the helicase activity of eIF4A by eIF4B, eIF4H, and eIF4F
    G W Rogers
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4935, USA
    J Biol Chem 276:30914-22. 2001
    ..A simple model of how eIF4B or eIF4H affects the duplex unwinding mechanism of eIF4A is proposed...
  82. pmc Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein
    Klaus Forstemann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 3:e236. 2005
    ..Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis...
  83. pmc Dead-box proteins: a family affair--active and passive players in RNP-remodeling
    Patrick Linder
    Department of Microbiology and Molecular Medicine, CMU 1, rue Michel Servet, CH 1211 Geneve 4, Switzerland
    Nucleic Acids Res 34:4168-80. 2006
    ..Here, I shall give a general overview of the DEAD-box protein family...
  84. pmc Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination
    Junya Kawauchi
    Sir William Dunn School of Pathology, Oxford OX1 3RE, United Kingdom
    Genes Dev 22:1082-92. 2008
    ....
  85. ncbi RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer
    M R Culbertson
    Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
    Trends Genet 15:74-80. 1999
    ..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
  86. pmc Interaction between Nmd2p and Upf1p is required for activity but not for dominant-negative inhibition of the nonsense-mediated mRNA decay pathway in yeast
    F He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655 0122, USA
    RNA 2:153-70. 1996
    ....
  87. ncbi Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein
    Chun Tang Chiou
    Graduate Institute of Life Sciences, National Defense Medical Center, 161 MinChuan E Rd Sec 6, Taipei 114, Taiwan
    J Gen Virol 84:2795-805. 2003
    ..Our observations indicate that microtubules and TSG101 associate with NS3, which is incorporated into the JEV-induced structure during JEV replication...
  88. pmc Cardiac deletion of Smyd2 is dispensable for mouse heart development
    Florian Diehl
    Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Hessen, Germany
    PLoS ONE 5:e9748. 2010
    ....
  89. pmc RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance
    Zhizhong Gong
    Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
    Proc Natl Acad Sci U S A 99:11507-12. 2002
    ..Our results identify a regulator of CBF genes, and demonstrate the importance of gene regulation and the CBF transcriptional activators in plant chilling resistance...
  90. pmc Complementation analysis of the flavivirus Kunjin NS3 and NS5 proteins defines the minimal regions essential for formation of a replication complex and shows a requirement of NS3 in cis for virus assembly
    Wen Jun Liu
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, and Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland 4029, Australia
    J Virol 76:10766-75. 2002
    ..The results define minimal regions in the NS3 and NS5 genes essential for the formation of complementable replication complex and show a requirement of NS3 in cis for virus assembly...
  91. doi Functional role for senataxin, defective in ataxia oculomotor apraxia type 2, in transcriptional regulation
    Amila Suraweera
    Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    Hum Mol Genet 18:3384-96. 2009
    ..These data suggest that senataxin, similar to its yeast homolog Sen1p, plays a role in coordinating transcriptional events, in addition to its role in DNA repair...
  92. pmc Dual roles of RNA helicase A in CREB-dependent transcription
    S Aratani
    Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mol Cell Biol 21:4460-9. 2001
    ..These results suggest that RHA could independently regulate CREB-dependent transcription either through recruitment of Pol II or by ATP-dependent mechanisms...
  93. ncbi RNA helicase A interacts with nuclear factor kappaB p65 and functions as a transcriptional coactivator
    Toshifumi Tetsuka
    Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan
    Eur J Biochem 271:3741-51. 2004
    ..These data suggest that RHA is an essential component of the transactivation complex by mediating the transcriptional activity of NF-kappaB...
  94. ncbi The DEAD-box protein family of RNA helicases
    Olivier Cordin
    Département de Microbiologie et Médecine Moléculaire, Centre Medical Universitaire, 1 rue Michel Servet, 1211, Geneve 4, Switzerland
    Gene 367:17-37. 2006
    b>RNA helicases of the DEAD-box protein family have been shown to participate in every aspect of RNA metabolism. They are present in most organisms where they work as RNA helicases or RNPases...
  95. ncbi Dicer is essential for mouse development
    Emily Bernstein
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 35:215-7. 2003
    ..Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development...
  96. pmc Endogenous RNA interference provides a somatic defense against Drosophila transposons
    Wei Jen Chung
    Department of Developmental Biology, Sloan Kettering Institute, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA
    Curr Biol 18:795-802. 2008
    ..The piRNA system is thought to provide primarily a germline defense against TE activity...
  97. pmc miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
    Zissimos Mourelatos
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Genes Dev 16:720-8. 2002
    ..The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem-loop structures...
  98. pmc Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli
    P G Jones
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA
    Proc Natl Acad Sci U S A 93:76-80. 1996
    ..Therefore, the requirement for CsdA in derepression of heat-shock protein synthesis is a cold shock-induced function possibly mediated by destabilization of secondary structures previously identified in the rpoH mRNA...
  99. doi Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells
    Yoshinori Kawamura
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Nature 453:793-7. 2008
    ..Mutations in Dicer-2 caused an increase in retrotransposon transcripts. Together, our findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila...
  100. pmc Understanding helicases as a means of virus control
    D N Frick
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Curr Pharm Des 12:1315-38. 2006
    ....
  101. ncbi Overexpression of a protein fragment of RNA helicase A causes inhibition of endogenous BRCA1 function and defects in ploidy and cytokinesis in mammary epithelial cells
    Brian P Schlegel
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 22:983-91. 2003
    ..Furthermore, inhibition of BRCA1 via overexpressing the RHA fragment coincides with a reduction in PARP-1 protein expression, suggesting a possible mechanism for BRCA1 in the maintenance of genomic integrity...

Research Grants76

  1. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2007
    ..The tractable in vitro and in vivo TBSV system developed by the investigator could prove beneficial to studies of other, less amenable RNA viruses. ..
  2. Structure and Function of the Hepatitis C Virus Genome
    PETER contact SARNOW; Fiscal Year: 2010
    ..To do this, novel biochemical, structural and genetic approaches will be employed to study protein-HCV RNA interactions. ..
  3. Antiviral potential of helicase inhibitors
    David N Frick; Fiscal Year: 2010
    ..We will also develop similar assays to identify compounds that might inhibit viruses that cause AIDS, yellow fever, Dengue fever, West Nile and Japanese encephalitis. ..
  4. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2010
    ..Pathways and enzymes that regulate the abundance, lifetime, and processing of RNA have been associated with human diseases that include neurodegenerative disorders, cancer, and inflammation. ..
  5. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 1993
    ..Adjacent to this domain, there are regions of similarity to several RNA helicases, and to replication proteins...
  6. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2007
    ..of either NTP or the 5'-triphosphorylated RNA and the recently reported crystal structures of YFV and DEN2 RNA helicases and those of hepatitis C virus and bovine viral diarrhea virus (BVDV) RdRP for docking studies...
  7. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..either NTP or the 5'-triphosphorylated RNA and the recently reported crystal structures of YFV and DEN2 RNA helicases and those of hepatitis C virus and bovine viral diarrhea virus (BVDV) RdRP for docking studies...
  8. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 2003
    ..4. NS3/NS5 interaction is regulated by phosphorylation and subcellular location of NS5. The PI proposes to test the hypothesis that the nuclear NS5 regulates the expression of cellular gene(s) in response to viral infection. ..
  9. MOLECULAR BIOLOGY OF POLIOVIRUS RNA REPLICATION
    JAMES FLANEGAN; Fiscal Year: 2003
    ..5) Investigate the mechanisms involved in the initiation of viral RNA synthesis including the template priming: VPg- linkage model and the VPg-priming model. ..
  10. Protein Arginine Methylation in Trypanosomes
    Laurie K Read; Fiscal Year: 2010
    ..In addition, because the effects of arginine methylation on RNA metabolism are not well understood in general, they will also provide insight into a common posttranslational modification in higher eukaryotes. ..
  11. STRUCTURE BASED ANALYSIS OF DENGUE VIRUS SERINE PROTEASE
    Krishna Murthy; Fiscal Year: 2004
    ..It will also permit a structural definition of alternate targets for inhibition of the protease and their detailed evaluation. ..
  12. "Analyses of two genes that affect plasmodesmata structure and function"
    Patricia Zambryski; Fiscal Year: 2007
    ..ISE1 and ISE2 encode two different types of RNA helicases. ISE1 localizes to PD, and ISE2 localizes to cytoplasmic granules resembling RNA processing bodies...
  13. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2010
    ..A better understanding of circadian clocks will potentially lead to new therapeutic approaches for treating human diseases. ..
  14. Mechanisms of Posttranscriptional Gene Silencing
    Heriberto Cerutti; Fiscal Year: 2004
    ..If the silencing mechanisms are indeed effective as anti-transposon and anti-viral agents, their further elucidation will have impact not only in basic biology but also in medicine and agriculture. ..
  15. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..The overall findings are expected to improve our ability to exploit RNAi as an experimental and/or therapeutic tool, with likely impacts in both medicine and agriculture. ..
  16. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2007
    ..The overall findings are expected to improve our ability to exploit RNAi as an experimental and/or therapeutic tool, with likely impacts in both medicine and agriculture. ..
  17. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2009
    ....
  18. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    Jeffrey Bonanno; Fiscal Year: 2002
    ..Specific Aim 6. Determine X-ray structures of PABP recognizing poly (A) RNA, and various binary and ternary complexes with a PABP-interacting protein and eIF4G. ..
  19. FUNCTIONAL SPLICEOSOME AND EXTRINSIC SPLICING FACTORS
    Ren Jang Lin; Fiscal Year: 2006
    ..We will analyze the splicing machinery when DBP2 function is impaired in vivo or in vitro. The proposed study shall reveal the dynamic features and the remodeling of the spliceosome core in yeast and human. ..
  20. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..Specific Aim 6. Determine X-ray structures of PABP recognizing poly (A) RNA, and various binary and ternary complexes with a PABP-interacting protein and eIF4G. ..
  21. Biochemical Characterization of p68 RNA Helicase
    Zhi Ren Liu; Fiscal Year: 2007
    ..A number of putative RNA helicases have been shown to function in the spliceosome...
  22. Expression and function of the let-7 RNA in C. elegans
    Amy Pasquinelli; Fiscal Year: 2007
    ..B) The association of factors with the lin-41 3'UTR sequences regulated by let-7 will be characterized using in vitro synthesized RNA probes and C. elegans extracts. ..
  23. Regulation of Type I Collagen in Hepatic Fibrosis
    Branko Stefanovic; Fiscal Year: 2009
    ..This proposal will characterize the newly discovered mechanism of collagen synthesis, which is dependent on structural protein myosin. ..
  24. Inhibitors of poxvirus enzymes as novel drugs
    ESTEBAN MENA; Fiscal Year: 2007
    ..in fungal ecology and natural product extract acquisition, Dr. Stewart Shuman in poxvirus enzymology and molecular biology, and Research Triangle Institute in natural product dru 9 discovery and medicinal chemistry. ..
  25. CELLULAR FACTORS INVOLVED IN NUCLEAR EXPORT OF VIRAL RNA
    Flossie Wong Staal; Fiscal Year: 2001
    ....