rna helicases

Summary

Summary: A family of proteins that promote unwinding of RNA during splicing and translation. EC 2.7.7.-.

Top Publications

  1. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
  2. ncbi Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
  3. ncbi RNA chaperones, RNA annealers and RNA helicases
    Lukas Rajkowitsch
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    RNA Biol 4:118-30. 2007
  4. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
  5. ncbi Innate immune recognition of viral infection
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Suita, Osaka 565-0871, Japan
    Nat Immunol 7:131-7. 2006
  6. ncbi Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland
    Mol Cell 22:561-9. 2006
  7. ncbi The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:795-804. 2008
  8. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
  9. ncbi IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency
    Nat Immunol 6:981-8. 2005
  10. ncbi Quantitative expression of RIG-like helicase, NOD-like receptor and inflammasome-related mRNAs in humans and mice
    Maciej Lech
    Medizinische Poliklinik, University of Munich, 80336 Munich, Germany
    Int Immunol 22:717-28. 2010

Research Grants

  1. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2007
  2. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2010
  3. Structure and Function of the Hepatitis C Virus Genome
    PETER contact SARNOW; Fiscal Year: 2010
  4. Antiviral potential of helicase inhibitors
    David N Frick; Fiscal Year: 2010
  5. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 1993
  6. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2007
  7. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
  8. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 2003
  9. MOLECULAR BIOLOGY OF POLIOVIRUS RNA REPLICATION
    JAMES FLANEGAN; Fiscal Year: 2003
  10. Protein Arginine Methylation in Trypanosomes
    Laurie K Read; Fiscal Year: 2010

Detail Information

Publications283 found, 100 shown here

  1. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
    ..Subsequent gene activation by these factors induced antiviral functions, including type I interferon production. Thus, RIG-I is key in the detection and subsequent eradication of the replicating viral genomes...
  2. ncbi Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
    ..within infected cells and is sensed by Toll-like receptor 3 (TLR3) and the recently identified cytosolic RNA helicases RIG-I (retinoic acid inducible gene I, also known as Ddx58) and Mda5 (melanoma differentiation-associated gene ..
  3. ncbi RNA chaperones, RNA annealers and RNA helicases
    Lukas Rajkowitsch
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    RNA Biol 4:118-30. 2007
    ..DExD/H-box containing RNA helicases are discussed as a special family of enzymes that restructure RNA or RNPs in an ATP-dependent manner...
  4. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  5. ncbi Innate immune recognition of viral infection
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Suita, Osaka 565-0871, Japan
    Nat Immunol 7:131-7. 2006
    ..Cells also express cytoplasmic RNA helicases that function as alternative pattern-recognition receptors through recognition of double-stranded RNA produced ..
  6. ncbi Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland
    Mol Cell 22:561-9. 2006
    ..Recently, a second system has been described, which relies on the cytoplasmic recognition of dsRNA by RNA helicases such as RIG-I...
  7. ncbi The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:795-804. 2008
    ..Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3...
  8. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
    ....
  9. ncbi IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency
    Nat Immunol 6:981-8. 2005
    ..CARD-like structure that mediated interaction with the CARD of RIG-I and Mda5, which are cytoplasmic RNA helicases that sense viral infection...
  10. ncbi Quantitative expression of RIG-like helicase, NOD-like receptor and inflammasome-related mRNAs in humans and mice
    Maciej Lech
    Medizinische Poliklinik, University of Munich, 80336 Munich, Germany
    Int Immunol 22:717-28. 2010
    ..These data suggest that the cell-type-, organ- and species-specific expression and regulation need to be considered in the design and interpretation of related studies...
  11. ncbi Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila
    Joao Trindade Marques
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois, USA
    Nat Struct Mol Biol 17:24-30. 2010
    ..Loqs is primarily required for dsRNA processing, whereas R2D2 is essential for the subsequent loading of siRNAs into effector Ago-RISC complexes...
  12. ncbi Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes
    Kui Li
    Department of Microbiology and Immunology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas 77555 1019, USA
    J Biol Chem 280:16739-47. 2005
    ..We conclude that hepatocytes contain two distinct antiviral signaling pathways leading to expression of type I IFNs, one dependent upon TLR3 and the other dependent on RIG-I, with little cross-talk between these pathways...
  13. ncbi Identification and functional analysis of RNase E of Vibrio angustum S14 and two-hybrid analysis of its interaction partners
    Melissa A Erce
    Systems Biology Laboratory, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia
    Biochim Biophys Acta 1794:1107-14. 2009
    ..The role of RNase E as a hub protein and the implications of microdomain-mediated interactions in relation to specificity and function are discussed...
  14. ncbi The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
    Katsutomo Okamura
    Sloan Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Avenue, Box 252, New York, New York 10065, USA
    Nature 453:803-6. 2008
    ..These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila...
  15. ncbi Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein
    Dahai Luo
    Structural and Computational Biology Division, School of Biological Sciences, Nanyang Technological University, Singapore
    EMBO J 27:3209-19. 2008
    ..They also suggest how NS3 could exert an effect as an RNA-anchoring device and thus participate both in flavivirus RNA replication and assembly...
  16. ncbi The requirement for eukaryotic initiation factor 4A (elF4A) in translation is in direct proportion to the degree of mRNA 5' secondary structure
    Y V Svitkin
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada
    RNA 7:382-94. 2001
    ..elF4A possesses ATPase and RNA helicase activities and is the prototype for a large family of putative RNA helicases (the DEAD box family)...
  17. ncbi Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells
    C Yoon
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    Development 124:3157-65. 1997
    ..Furthermore, they provide the basis for further studies on this novel RNA localization pattern and on germ-line development in general...
  18. ncbi Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity
    Mitsutoshi Yoneyama
    Antiviral Innate Immunity Project, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Tokyo, Japan
    J Immunol 175:2851-8. 2005
    ..These results highlight ingenious mechanisms for initiating antiviral innate immune responses and the action of virus-encoded inhibitors...
  19. ncbi mRNAs encoding telomerase components and regulators are controlled by UPF genes in Saccharomyces cerevisiae
    Jeffrey N Dahlseid
    Department of Chemistry, St Olaf College, Northfield, Minnesota 55057, USA
    Eukaryot Cell 2:134-42. 2003
    ..Together, these results suggest that NMD maintains the balance of gene products that control telomere length and telomeric silencing primarily by maintaining appropriate levels of STN1, TEN1, and EST2 mRNA...
  20. ncbi Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination
    Junya Kawauchi
    Sir William Dunn School of Pathology, Oxford OX1 3RE, United Kingdom
    Genes Dev 22:1082-92. 2008
    ....
  21. ncbi RISC assembly defects in the Drosophila RNAi mutant armitage
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 116:831-41. 2004
    ..Our results suggest that armi is required for RISC maturation...
  22. ncbi Senataxin, defective in ataxia oculomotor apraxia type 2, is involved in the defense against oxidative DNA damage
    Amila Suraweera
    Radiation Biology and Oncology Laboratory, Queensland Institute of Medical Research, Brisbane, QLD 4029, Australia
    J Cell Biol 177:969-79. 2007
    ..These results provide evidence that an additional member of the autosomal recessive AOA is also characterized by a defective response to DNA damage, which may contribute to the neurodegeneration seen in this syndrome...
  23. ncbi Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein
    Klaus Forstemann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 3:e236. 2005
    ..Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis...
  24. ncbi Modulation of the helicase activity of eIF4A by eIF4B, eIF4H, and eIF4F
    G W Rogers
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4935, USA
    J Biol Chem 276:30914-22. 2001
    ..A simple model of how eIF4B or eIF4H affects the duplex unwinding mechanism of eIF4A is proposed...
  25. ncbi RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer
    M R Culbertson
    Laboratory of Genetics, R M Bock Laboratories, University of Wisconsin, Madison 53706, USA
    Trends Genet 15:74-80. 1999
    ..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
  26. ncbi Dead-box proteins: a family affair--active and passive players in RNP-remodeling
    Patrick Linder
    Department of Microbiology and Molecular Medicine, CMU 1, rue Michel Servet, CH 1211 Geneve 4, Switzerland
    Nucleic Acids Res 34:4168-80. 2006
    ..Here, I shall give a general overview of the DEAD-box protein family...
  27. ncbi Interaction between Nmd2p and Upf1p is required for activity but not for dominant-negative inhibition of the nonsense-mediated mRNA decay pathway in yeast
    F He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655 0122, USA
    RNA 2:153-70. 1996
    ....
  28. ncbi RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance
    Zhizhong Gong
    Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
    Proc Natl Acad Sci U S A 99:11507-12. 2002
    ..Our results identify a regulator of CBF genes, and demonstrate the importance of gene regulation and the CBF transcriptional activators in plant chilling resistance...
  29. ncbi Complementation analysis of the flavivirus Kunjin NS3 and NS5 proteins defines the minimal regions essential for formation of a replication complex and shows a requirement of NS3 in cis for virus assembly
    Wen Jun Liu
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, and Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland 4029, Australia
    J Virol 76:10766-75. 2002
    ..The results define minimal regions in the NS3 and NS5 genes essential for the formation of complementable replication complex and show a requirement of NS3 in cis for virus assembly...
  30. ncbi Functional role for senataxin, defective in ataxia oculomotor apraxia type 2, in transcriptional regulation
    Amila Suraweera
    Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    Hum Mol Genet 18:3384-96. 2009
    ..These data suggest that senataxin, similar to its yeast homolog Sen1p, plays a role in coordinating transcriptional events, in addition to its role in DNA repair...
  31. ncbi Association of Japanese encephalitis virus NS3 protein with microtubules and tumour susceptibility gene 101 (TSG101) protein
    Chun Tang Chiou
    Graduate Institute of Life Sciences, National Defense Medical Center, 161 MinChuan E Rd Sec 6, Taipei 114, Taiwan
    J Gen Virol 84:2795-805. 2003
    ..Our observations indicate that microtubules and TSG101 associate with NS3, which is incorporated into the JEV-induced structure during JEV replication...
  32. ncbi LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses
    Takashi Satoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:1512-7. 2010
    ..Taken together, the present data suggest that LGP2 facilitates viral RNA recognition by RIG-I and MDA5 through its ATPase domain...
  33. ncbi RNA helicases at work: binding and rearranging
    Eckhard Jankowsky
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Trends Biochem Sci 36:19-29. 2011
    b>RNA helicases are ubiquitous, highly conserved enzymes that participate in nearly all aspects of RNA metabolism. These proteins bind or remodel RNA or RNA-protein complexes in an ATP-dependent fashion...
  34. ncbi Dual roles of RNA helicase A in CREB-dependent transcription
    S Aratani
    Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
    Mol Cell Biol 21:4460-9. 2001
    ..These results suggest that RHA could independently regulate CREB-dependent transcription either through recruitment of Pol II or by ATP-dependent mechanisms...
  35. ncbi Yeast Sen1 helicase protects the genome from transcription-associated instability
    Hannah E Mischo
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 41:21-32. 2011
    ..Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability...
  36. ncbi Cardiac deletion of Smyd2 is dispensable for mouse heart development
    Florian Diehl
    Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Hessen, Germany
    PLoS ONE 5:e9748. 2010
    ....
  37. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage...
  38. ncbi RNA helicase A interacts with nuclear factor kappaB p65 and functions as a transcriptional coactivator
    Toshifumi Tetsuka
    Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan
    Eur J Biochem 271:3741-51. 2004
    ..These data suggest that RHA is an essential component of the transactivation complex by mediating the transcriptional activity of NF-kappaB...
  39. ncbi The DEAD-box protein family of RNA helicases
    Olivier Cordin
    , , 1 rue Michel Servet, 1211, , Switzerland
    Gene 367:17-37. 2006
    b>RNA helicases of the DEAD-box protein family have been shown to participate in every aspect of RNA metabolism. They are present in most organisms where they work as RNA helicases or RNPases...
  40. ncbi Dicer is essential for mouse development
    Emily Bernstein
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Genet 35:215-7. 2003
    ..Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development...
  41. ncbi Endogenous RNA interference provides a somatic defense against Drosophila transposons
    Wei Jen Chung
    Department of Developmental Biology, Sloan Kettering Institute, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA
    Curr Biol 18:795-802. 2008
    ..The piRNA system is thought to provide primarily a germline defense against TE activity...
  42. ncbi Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli
    P G Jones
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA
    Proc Natl Acad Sci U S A 93:76-80. 1996
    ..Therefore, the requirement for CsdA in derepression of heat-shock protein synthesis is a cold shock-induced function possibly mediated by destabilization of secondary structures previously identified in the rpoH mRNA...
  43. ncbi miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
    Zissimos Mourelatos
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Genes Dev 16:720-8. 2002
    ..The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem-loop structures...
  44. ncbi Understanding helicases as a means of virus control
    D N Frick
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Curr Pharm Des 12:1315-38. 2006
    ....
  45. ncbi Single processing center models for human Dicer and bacterial RNase III
    Haidi Zhang
    Friedrich Miescher Institute for Biomedical Research, PO Box 2543, 4002 Basel, Switzerland
    Cell 118:57-68. 2004
    ..Based on these and other data, we propose that Dicer functions through intramolecular dimerization of its two RNase III domains, assisted by the flanking RNA binding domains, PAZ and dsRBD...
  46. ncbi Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression
    Concetta Lipardi
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Building 37, Room 6118, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:15645-50. 2009
    ..The generality of this mechanism in genome defense and RNA silencing in higher eukaryotes is suggested...
  47. ncbi Overexpression of a protein fragment of RNA helicase A causes inhibition of endogenous BRCA1 function and defects in ploidy and cytokinesis in mammary epithelial cells
    Brian P Schlegel
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 22:983-91. 2003
    ..Furthermore, inhibition of BRCA1 via overexpressing the RHA fragment coincides with a reduction in PARP-1 protein expression, suggesting a possible mechanism for BRCA1 in the maintenance of genomic integrity...
  48. ncbi Mtt1 is a Upf1-like helicase that interacts with the translation termination factors and whose overexpression can modulate termination efficiency
    K Czaplinski
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA
    RNA 6:730-43. 2000
    ..Taken together, these results indicate that at least two members of a family of RNA helicases modulate translation termination efficiency in cells.
  49. ncbi DEAD-box proteins: the driving forces behind RNA metabolism
    Sanda Rocak
    , , 1 rue Michel Servet, CH-1211 Geneva 4, Switzerland
    Nat Rev Mol Cell Biol 5:232-41. 2004
  50. ncbi HrpA, a DEAH-box RNA helicase, is involved in mRNA processing of a fimbrial operon in Escherichia coli
    Jovanka T Koo
    Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195 7242, USA
    Mol Microbiol 52:1813-26. 2004
    ..We propose that HrpA is a novel enzyme involved in mRNA processing in E. coli...
  51. ncbi Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells
    Yoshinori Kawamura
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan
    Nature 453:793-7. 2008
    ..Mutations in Dicer-2 caused an increase in retrotransposon transcripts. Together, our findings indicate that different types of small RNAs and Argonautes are used to repress retrotransposons in germline and somatic cells in Drosophila...
  52. ncbi Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast
    Shinya Takahashi
    Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113 0033, Japan
    RNA 14:1950-8. 2008
    ..These results suggest that Upf1 may serve as an E3 ubiquitin ligase upon its association with Upf3 and play an important role in signaling to the NMD pathway...
  53. ncbi Dbp9p, a member of the DEAD box protein family, exhibits DNA helicase activity
    Takashi Kikuma
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    J Biol Chem 279:20692-8. 2004
    The yeast Dbp9p is a member of the DEAD box family of RNA helicases, which are thought to be involved in RNA metabolism. Dbp9p seems to function in ribosomal RNA biogenesis, but it has not been biochemically characterized...
  54. ncbi Plant RNA helicases: linking aberrant and silencing RNA
    Patrick Linder
    Department of Microbiology and Molecular Medicine, CMU, 1 rue Michel Servet, CH 1211 Geneve 4, Switzerland
    Trends Plant Sci 14:344-52. 2009
    b>RNA helicases are ATPases that are capable of rearranging RNA and ribonucleoprotein (RNP) structure, and they can potentially function in any aspect of RNA metabolism...
  55. ncbi Structural basis of double-stranded RNA recognition by the RIG-I like receptor MDA5
    Xiaojun Li
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
    Arch Biochem Biophys 488:23-33. 2009
    ..Molecular modeling of MDA5 CTD/dsRNA complex suggests that MDA5 CTD may recognize the first turn of blunt-ended dsRNA in a similar manner as LGP2...
  56. ncbi A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis
    Zhizhong Gong
    State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China
    Plant Cell 17:256-67. 2005
    ..These results demonstrate an important role of the CRYOPHYTE/LOS4 RNA helicase in mRNA export, plant development, and stress responses...
  57. ncbi Upf1p, a highly conserved protein required for nonsense-mediated mRNA decay, interacts with the nuclear pore proteins Nup100p and Nup116p
    Tara Nazarenus
    University of Nebraska Lincoln, School of Biological Sciences, E146 Beadle Center, Lincoln, Nebraska 68588 0666, USA
    Gene 345:199-212. 2005
    ....
  58. ncbi Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A
    Ting Xu
    School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, Singapore 637551
    J Virol 79:10278-88. 2005
    ..A possible model for the unwinding mechanism is proposed...
  59. ncbi The macroscopic rate of nucleic acid translocation by hepatitis C virus helicase NS3h is dependent on both sugar and base moieties
    Ali R Khaki
    Gilead Sciences, Inc, 333 Lakeside Drive, Foster City, CA 94404, USA
    J Mol Biol 400:354-78. 2010
    ..5 ATP molecule consumed per nucleotide translocated). This similar periodicity of ATP consumption implies a similar mechanism for NS3h translocation along RNA and DNA substrates...
  60. ncbi The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm
    Jian Zhao
    Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-17177 Stockholm, Sweden
    EMBO J 21:1177-87. 2002
    ..Finally, the addition of the export factor Dbp5 to the growing transcript highlights the importance of the co-transcriptional loading process in determining the fate of mRNA...
  61. ncbi Crystal structure of the catalytic domain of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase at a resolution of 1.8 A
    Tetsuo Yamashita
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Virology 373:426-36. 2008
    ..The detailed structural comparison among the viruses of the family Flaviviridae should help in clarifying the molecular mechanism of viral replication and in providing rationale for the development of appropriate therapeutics...
  62. ncbi The DExH/D protein family database
    E Jankowsky
    The Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Nucleic Acids Res 28:333-4. 2000
    ..columbia.edu/ ej67/dbhome.htm ). The database can be fully searched by text based queries, facilitating fast access to specific information about this important class of enzymes...
  63. ncbi Structure-based virtual screening for novel inhibitors of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase
    Agnieszka Kaczor
    Department of Synthesis and Chemical Technology of Medicinal Substances, Faculty of Pharmacy, Medical University of Lublin, Lublin, Poland
    FEMS Immunol Med Microbiol 58:91-101. 2010
    ..These compounds were docked to the JEV NS3 helicase/NTPase to examine their binding mode and verify screening results by consensus scoring procedure...
  64. ncbi Autogenous translational regulation of the Borna disease virus negative control factor X from polycistronic mRNA using host RNA helicases
    Yohei Watanabe
    Department of Virology, Research Institute for Microbial Diseases BIKEN, Osaka University, Suita, Osaka, Japan
    PLoS Pathog 5:e1000654. 2009
    ..Our results not only demonstrate a unique translational control of viral regulatory protein, but also elucidate a previously unknown mechanism of regulation of polycistronic mRNA translation using RNA helicases.
  65. ncbi The rRNA-processing function of the yeast U14 small nucleolar RNA can be rescued by a conserved RNA helicase-like protein
    W Q Liang
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003, USA
    Mol Cell Biol 17:4124-32. 1997
    ..Mediating the interaction of U14 with precursor 18S RNA is an especially attractive possibility...
  66. ncbi VASA mediates translation through interaction with a Drosophila yIF2 homolog
    P Carrera
    Abteilung Molekulare Entwicklungsbiologie Max Planck Institut für biophysikalische Chemie, Gottingen, Germany
    Mol Cell 5:181-7. 2000
    ..We conclude that VAS regulates translation of germline mRNAs by specific interaction with dIF2, an essential factor conserved from bacteria to humans...
  67. ncbi A cellular cofactor for the constitutive transport element of type D retrovirus
    H Tang
    Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Science 276:1412-5. 1997
    ..In vivo colocalization of helicase A and CTE was observed in experiments that combined in situ hybridization and immunostaining. These results suggest that helicase A plays a role in the nuclear export of CTE-containing RNA...
  68. ncbi An essential role for the Saccharomyces cerevisiae DEAD-box helicase DHH1 in G1/S DNA-damage checkpoint recovery
    Megan Bergkessel
    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Genetics 167:21-33. 2004
    ..Furthermore, the high degree of conservation between DHH1 and its human ortholog suggests that this mechanism is conserved among all eukaryotes and potentially important in human disease...
  69. ncbi Molecular models of NS3 protease variants of the Hepatitis C virus
    Nelson J F da Silveira
    Department of Physics, IBILCE UNESP, Sao Jose do Rio Preto, Sao Paulo, Brazil
    BMC Struct Biol 5:1. 2005
    ..In order to speed up the modeling process all NS3 protease variant models were generated in a Beowulf cluster. The potential of the structural bioinformatics for development of new antiviral drugs is discussed...
  70. ncbi The NUG1 GTPase reveals and N-terminal RNA-binding domain that is essential for association with 60 S pre-ribosomal particles
    Jochen Bassler
    Biochemie Zentrum der Universitat Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 281:24737-44. 2006
    ..Our findings suggest that Nug1 associates with pre-60 S subunits via its essential N-terminal RNA-binding domain and exerts a non-essential regulative role in pre-60 S subunit biogenesis via its central GTPase domain...
  71. ncbi Enzymatic characterization and homology model of a catalytically active recombinant West Nile virus NS3 protease
    Tessa A Nall
    Centre for Drug Design and Development, Institute for Molecular Bioscience, Univeristy of Queensland, Brisbane, Australia
    J Biol Chem 279:48535-42. 2004
    ....
  72. ncbi A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae
    Udo Schmidt
    Department of Microbiology and Genetics, Faculty III, University of Technology Berlin, Gustav Meyer Allee 25, Germany
    FEMS Yeast Res 2:267-76. 2002
    ..The gene encodes the fourth putative RNA helicase of Saccharomyces cerevisiae implicated in a mitochondrial function and was therefore termed MRH4 (for mitochondrial RNA helicase)...
  73. ncbi Role for Upf2p phosphorylation in Saccharomyces cerevisiae nonsense-mediated mRNA decay
    Weirong Wang
    Department of Biology, University of Puerto Rico, San Juan, PR 00931
    Mol Cell Biol 26:3390-400. 2006
    ..Our results indicate that phosphorylation of UPF1 and UPF2 is a conserved event in eukaryotes and for the first time provide evidence that Upf2p phosphorylation is crucial for NMD...
  74. ncbi Structure of the Flavivirus helicase: implications for catalytic activity, protein interactions, and proteolytic processing
    Jinhua Wu
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Virol 79:10268-77. 2005
    ..The third, C-terminal domain has a unique structure and is proposed to function in RNA and protein recognition. The organization of the three domains indicates that cleavage of the viral polyprotein NS3-NS4A junction occurs in trans...
  75. ncbi A truncated form of KlLsm4p and the absence of factors involved in mRNA decapping trigger apoptosis in yeast
    Cristina Mazzoni
    Pasteur Institute Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome, Italy
    Mol Biol Cell 14:721-9. 2003
    ..This is the first time that a connection between mRNA stability and apoptosis is reported in yeast, pointing to mRNA decapping as the crucial step responsible of the observed apoptotic phenotypes...
  76. ncbi The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase
    Andrei V Chernov
    Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Biol Chem 283:17270-8. 2008
    ....
  77. ncbi Two RNA binding proteins, HEN4 and HUA1, act in the processing of AGAMOUS pre-mRNA in Arabidopsis thaliana
    Yulan Cheng
    Waksman Institute, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
    Dev Cell 4:53-66. 2003
    ..Our studies underscore the importance of RNA processing in modulating plant development...
  78. ncbi Upf1p, Nmd2p, and Upf3p regulate the decapping and exonucleolytic degradation of both nonsense-containing mRNAs and wild-type mRNAs
    F He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 0122, USA
    Mol Cell Biol 21:1515-30. 2001
    ..The participation of the NMD factors in general mRNA degradation suggests that they may regulate an aspect of translation termination common to all transcripts...
  79. ncbi The nonstructural protein 3 protease/helicase requires an intact protease domain to unwind duplex RNA efficiently
    David N Frick
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 279:1269-80. 2004
    ..The data suggest that hepatitis C virus RNA helicase, and therefore viral replication, could be influenced by the rotations of the protease domain which likely occur during polyprotein processing...
  80. ncbi Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae
    E Martegani
    Dipartimento di Fisiologia e Biochimica Generali, Universita di Milano, Italy
    Yeast 13:391-7. 1997
    ..cerevisiae and contains domains characteristics of RNA-helicases. The ORF is transcribed in vegetative cells but it is not essential for viability as demonstrated by gene disruption...
  81. ncbi dsRBM1 and a proline-rich domain of RNA helicase A can form a composite binder to recognize a specific dsDNA
    Ming-Lung Hung
    Institute of Biochemistry, National Chung-Hsing University, 250 Kuo-Kung Road, Taichung 402, Taiwan
    Nucleic Acids Res 31:5741-53. 2003
    ....
  82. ncbi R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway
    Qinghua Liu
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Science 301:1921-5. 2003
    ..These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC...
  83. ncbi The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export
    V Del Priore
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Mol Biol Cell 7:1601-21. 1996
    ....
  84. ncbi Processing of a pestivirus protein by a cellular protease specific for light chain 3 of microtubule-associated proteins
    Jens Fricke
    Institut fur Immunologie, Federal Research Center for Virus Diseases of Animals, P O Box 1149, D 72001 Tübingen, Germany
    J Virol 78:5900-12. 2004
    ....
  85. ncbi Mammalian orthologues of a yeast regulator of nonsense transcript stability
    H A Perlick
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 93:10928-32. 1996
    ..Thus, despite apparent differences between yeast and mammalian nonsense-mediated RNA decay, these data suggest that the two pathways use functionally related machinery...
  86. ncbi Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein
    S E Applequist
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University of Chicago, Maywood, IL 60153, USA
    Nucleic Acids Res 25:814-21. 1997
    ..UPF1 and its human homolog also share similar putative zinc finger motifs that are absent in other group I RNA helicases. Northern blot analysis with the human cDNA probe revealed two transcripts in several human cell lines...
  87. ncbi Conserved amino acids 193-324 of non-structural protein 3 are a dominant source of peptide determinants for CD4+ and CD8+ T cells in a healthy Japanese encephalitis virus-endemic cohort
    Priti Kumar
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka 560012, India
    J Gen Virol 85:1131-43. 2004
    ....
  88. ncbi Identification and characterization of mutations in the UPF1 gene that affect nonsense suppression and the formation of the Upf protein complex but not mRNA turnover
    Y Weng
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, University of Medicine, Piscataway 08854, USA
    Mol Cell Biol 16:5491-506. 1996
    ..Mutations in the cysteine- and histidine-rich region of Upf1p abolish Upf1p-Upf2p interaction. On the basis of these results, the role of the Upf complex in nonsense-mediated mRNA decay and nonsense suppression is discussed...
  89. ncbi Prp43: An RNA helicase-like factor involved in spliceosome disassembly
    J E Arenas
    Division of Biology, 147 75, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 94:11798-802. 1997
    ..PRP22 encode pre-mRNA splicing factors that belong to the highly conserved "DEAH" family of putative RNA helicases. We previously identified two additional members of this family, JA1 and JA2...
  90. ncbi Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae
    Svetlana Chabelskaya
    Department of Genetics and Breeding, St Petersburg State University, Universitetskaya emb, 7 9, 199034, St Petersburg, Russia
    BMC Mol Biol 8:71. 2007
    ....
  91. ncbi A DEAD-box-family protein is required for nucleocytoplasmic transport of yeast mRNA
    S Liang
    Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Mol Cell Biol 16:5139-46. 1996
    ..Mtr4p shares characteristic motifs with DEAD-box RNA helicases and associates with RNA. It therefore may well affect RNA conformation...
  92. ncbi Preliminary crystallographic characterization of an RNA helicase from Kunjin virus
    Eloise Mastrangelo
    Department of Biomolecular Sciences and Biotechnology, CNR INFM, University of Milano, Via Celoria 26, 20133 Milano, Italy
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:876-9. 2006
    ..Kunjin virus NS3 helicase domain is an attractive model for studying the molecular mechanisms of flavivirus replication, while simultaneously providing a new basis for the rational development of anti-flaviviral compounds...
  93. ncbi Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense-mediated mRNA decay
    Brian Luke
    Swiss Institute for Experimental Cancer Research ISREC, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    Nucleic Acids Res 35:7688-97. 2007
    ..Overall our findings suggest that NMD is more conserved in evolution than previously thought, and that at least one of the Smg5-7 proteins is conserved in budding yeast...
  94. ncbi Dengue 2 virus NS2B and NS3 form a stable complex that can cleave NS3 within the helicase domain
    C F Arias
    Division of Biology, California Institute of Technology, Pasadena 91125
    Virology 193:888-99. 1993
    ..The importance of this alternatively processed form of NS3 and its role in the replication cycle of dengue virus remain to be determined...
  95. ncbi How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing
    Shelly Ann James
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 10021, USA
    RNA 8:1068-77. 2002
    ..All three proteins are released from the spliceosome after step 2 concomitant with release of mature mRNA...
  96. ncbi Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen
    F He
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655 0122
    Genes Dev 9:437-54. 1995
    ..The association of a dominant-negative phenotype with a gene fragment identified in a two-hybrid screen suggests a generalized approach to confirming the function of genes identified in such screens...
  97. ncbi Ebs1p, a negative regulator of gene expression controlled by the Upf proteins in the yeast Saccharomyces cerevisiae
    Amanda S Ford
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Eukaryot Cell 5:301-12. 2006
    ..Finally, EBS1 transcript levels are under the control of nonsense-mediated mRNA decay (NMD), providing the first example of an NMD-sensitive transcript whose protein product influences a step in gene expression required for NMD...
  98. ncbi SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast
    M F Page
    Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA
    Mol Cell Biol 19:5943-51. 1999
    ..We discuss these results with regard to the in vivo functions of SMG-2 and NMD...
  99. ncbi [Epitope mapping of antigenic determinants of hepatitis C virus proteins by phage display]
    E A Rechkina
    Mol Biol (Mosk) 40:357-68. 2006
    ..The results could help to study an immune response in patients infected with different genotypes of HCV. Phage displayed peptides mimicking the antigenic epitopes of HCV proteins could be applied to development of HCV vaccine...
  100. ncbi The RNA aptamer-binding site of hepatitis C virus NS3 protease
    J Hwang
    National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, 305 8566, Japan
    Biochem Biophys Res Commun 279:557-62. 2000
    ..This region appears to be a potential targeting site for anti-HCV drugs...
  101. ncbi The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis
    T Nakagawa
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    EMBO J 18:5714-23. 1999
    ..MER3 appears to be very important for both the DSB transition and crossover control...

Research Grants75

  1. Roles of host RNA binding proteins in virus replication
    Peter Nagy; Fiscal Year: 2007
    ..The tractable in vitro and in vivo TBSV system developed by the investigator could prove beneficial to studies of other, less amenable RNA viruses. ..
  2. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2010
    ..Pathways and enzymes that regulate the abundance, lifetime, and processing of RNA have been associated with human diseases that include neurodegenerative disorders, cancer, and inflammation. ..
  3. Structure and Function of the Hepatitis C Virus Genome
    PETER contact SARNOW; Fiscal Year: 2010
    ..To do this, novel biochemical, structural and genetic approaches will be employed to study protein-HCV RNA interactions. ..
  4. Antiviral potential of helicase inhibitors
    David N Frick; Fiscal Year: 2010
    ..We will also develop similar assays to identify compounds that might inhibit viruses that cause AIDS, yellow fever, Dengue fever, West Nile and Japanese encephalitis. ..
  5. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 1993
    ..Adjacent to this domain, there are regions of similarity to several RNA helicases, and to replication proteins...
  6. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2007
    ..of either NTP or the 5'-triphosphorylated RNA and the recently reported crystal structures of YFV and DEN2 RNA helicases and those of hepatitis C virus and bovine viral diarrhea virus (BVDV) RdRP for docking studies...
  7. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..either NTP or the 5'-triphosphorylated RNA and the recently reported crystal structures of YFV and DEN2 RNA helicases and those of hepatitis C virus and bovine viral diarrhea virus (BVDV) RdRP for docking studies...
  8. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 2003
    ..4. NS3/NS5 interaction is regulated by phosphorylation and subcellular location of NS5. The PI proposes to test the hypothesis that the nuclear NS5 regulates the expression of cellular gene(s) in response to viral infection. ..
  9. MOLECULAR BIOLOGY OF POLIOVIRUS RNA REPLICATION
    JAMES FLANEGAN; Fiscal Year: 2003
    ..5) Investigate the mechanisms involved in the initiation of viral RNA synthesis including the template priming: VPg- linkage model and the VPg-priming model. ..
  10. Protein Arginine Methylation in Trypanosomes
    Laurie K Read; Fiscal Year: 2010
    ..In addition, because the effects of arginine methylation on RNA metabolism are not well understood in general, they will also provide insight into a common posttranslational modification in higher eukaryotes. ..
  11. STRUCTURE BASED ANALYSIS OF DENGUE VIRUS SERINE PROTEASE
    Krishna Murthy; Fiscal Year: 2004
    ..It will also permit a structural definition of alternate targets for inhibition of the protease and their detailed evaluation. ..
  12. "Analyses of two genes that affect plasmodesmata structure and function"
    Patricia Zambryski; Fiscal Year: 2007
    ..ISE1 and ISE2 encode two different types of RNA helicases. ISE1 localizes to PD, and ISE2 localizes to cytoplasmic granules resembling RNA processing bodies...
  13. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2010
    ..A better understanding of circadian clocks will potentially lead to new therapeutic approaches for treating human diseases. ..
  14. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..The overall findings are expected to improve our ability to exploit RNAi as an experimental and/or therapeutic tool, with likely impacts in both medicine and agriculture. ..
  15. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2007
    ..The overall findings are expected to improve our ability to exploit RNAi as an experimental and/or therapeutic tool, with likely impacts in both medicine and agriculture. ..
  16. Mechanisms of Posttranscriptional Gene Silencing
    Heriberto Cerutti; Fiscal Year: 2004
    ..If the silencing mechanisms are indeed effective as anti-transposon and anti-viral agents, their further elucidation will have impact not only in basic biology but also in medicine and agriculture. ..
  17. CHARACTERIZATION OF HCMV UL84
    Gregory Pari; Fiscal Year: 2009
    ....
  18. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..Specific Aim 6. Determine X-ray structures of PABP recognizing poly (A) RNA, and various binary and ternary complexes with a PABP-interacting protein and eIF4G. ..
  19. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    Jeffrey Bonanno; Fiscal Year: 2002
    ..Specific Aim 6. Determine X-ray structures of PABP recognizing poly (A) RNA, and various binary and ternary complexes with a PABP-interacting protein and eIF4G. ..
  20. FUNCTIONAL SPLICEOSOME AND EXTRINSIC SPLICING FACTORS
    Ren Jang Lin; Fiscal Year: 2006
    ..We will analyze the splicing machinery when DBP2 function is impaired in vivo or in vitro. The proposed study shall reveal the dynamic features and the remodeling of the spliceosome core in yeast and human. ..
  21. Biochemical Characterization of p68 RNA Helicase
    Zhi Ren Liu; Fiscal Year: 2007
    ..A number of putative RNA helicases have been shown to function in the spliceosome...
  22. Expression and function of the let-7 RNA in C. elegans
    Amy Pasquinelli; Fiscal Year: 2007
    ..B) The association of factors with the lin-41 3'UTR sequences regulated by let-7 will be characterized using in vitro synthesized RNA probes and C. elegans extracts. ..
  23. Regulation of Type I Collagen in Hepatic Fibrosis
    Branko Stefanovic; Fiscal Year: 2009
    ..This proposal will characterize the newly discovered mechanism of collagen synthesis, which is dependent on structural protein myosin. ..
  24. Inhibitors of poxvirus enzymes as novel drugs
    ESTEBAN MENA; Fiscal Year: 2007
    ..in fungal ecology and natural product extract acquisition, Dr. Stewart Shuman in poxvirus enzymology and molecular biology, and Research Triangle Institute in natural product dru 9 discovery and medicinal chemistry. ..
  25. CELLULAR FACTORS INVOLVED IN NUCLEAR EXPORT OF VIRAL RNA
    Flossie Wong Staal; Fiscal Year: 2001
    ....