rna helicases

Summary

Summary: A family of proteins that promote unwinding of RNA during splicing and translation. EC 2.7.7.-.

Top Publications

  1. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
  2. ncbi RNA degradation by the exosome is promoted by a nuclear polyadenylation complex
    John LaCava
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 121:713-24. 2005
  3. ncbi Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses
    Hiroki Kato
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Nature 441:101-5. 2006
  4. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008
  5. ncbi IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency
    Nat Immunol 6:981-8. 2005
  6. pmc SF1 and SF2 helicases: family matters
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 20:313-24. 2010
  7. ncbi Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa
    Toru Sengoku
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Cell 125:287-300. 2006
  8. pmc Yeast Sen1 helicase protects the genome from transcription-associated instability
    Hannah E Mischo
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 41:21-32. 2011
  9. ncbi R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway
    Qinghua Liu
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Science 301:1921-5. 2003
  10. pmc LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses
    Takashi Satoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:1512-7. 2010

Detail Information

Publications370 found, 100 shown here

  1. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
    ..Subsequent gene activation by these factors induced antiviral functions, including type I interferon production. Thus, RIG-I is key in the detection and subsequent eradication of the replicating viral genomes...
  2. ncbi RNA degradation by the exosome is promoted by a nuclear polyadenylation complex
    John LaCava
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell 121:713-24. 2005
    ..We speculate that this function was maintained in eukaryotic nuclei, while cytoplasmic mRNA poly(A) tails acquired different roles in translation...
  3. ncbi Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses
    Hiroki Kato
    Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Nature 441:101-5. 2006
    ..Together, our data show that RIG-I and MDA5 distinguish different RNA viruses and are critical for host antiviral responses...
  4. pmc An endogenous small interfering RNA pathway in Drosophila
    Benjamin Czech
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:798-802. 2008
    ..These observations expand the repertoire of small RNAs in Drosophila, adding a class that blurs distinctions based on known biogenesis mechanisms and functional roles...
  5. ncbi IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency
    Nat Immunol 6:981-8. 2005
    ..CARD-like structure that mediated interaction with the CARD of RIG-I and Mda5, which are cytoplasmic RNA helicases that sense viral infection...
  6. pmc SF1 and SF2 helicases: family matters
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 20:313-24. 2010
    ..We discuss recent data in the context of this unifying view of SF1 and SF2 helicases...
  7. ncbi Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa
    Toru Sengoku
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Cell 125:287-300. 2006
    DEAD-box RNA helicases, which regulate various processes involving RNA, have two RecA-like domains as a catalytic core to alter higher-order RNA structures. We determined the 2...
  8. pmc Yeast Sen1 helicase protects the genome from transcription-associated instability
    Hannah E Mischo
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 41:21-32. 2011
    ..Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability...
  9. ncbi R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway
    Qinghua Liu
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Science 301:1921-5. 2003
    ..These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC...
  10. pmc LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses
    Takashi Satoh
    Laboratory of Host Defense, WPI Immunology Frontier Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 107:1512-7. 2010
    ..Taken together, the present data suggest that LGP2 facilitates viral RNA recognition by RIG-I and MDA5 through its ATPase domain...
  11. pmc Small RNA-induced mRNA degradation achieved through both translation block and activated cleavage
    Karine Prévost
    Department of Biochemistry, RNA Group, University of Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Genes Dev 25:385-96. 2011
    ..Thus, beyond translation initiation block, sRNA-induced mRNA cleavage requires several unexpected steps, many of which are determined by structural features of the target mRNA...
  12. ncbi The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E
    Agamemnon J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaires, Unité Mixte de Recherche 5100, Centre National de la Recherche Scientifique et Université Paul Sabatier, 31062 Toulouse, France
    Annu Rev Microbiol 61:71-87. 2007
    ....
  13. pmc Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
    Megha Ghildiyal
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 320:1077-81. 2008
    ..We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line...
  14. ncbi Translocation and unwinding mechanisms of RNA and DNA helicases
    Anna Marie Pyle
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute and Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Biophys 37:317-36. 2008
    ..Through structural and single-molecule investigations, researchers are developing coherent models to explain the functions and dynamic motions of helicase enzymes...
  15. ncbi Ataxia with oculomotor apraxia type 2: clinical, biological and genotype/phenotype correlation study of a cohort of 90 patients
    M Anheim
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, Universite de Strasbourg, INSERM, Illkirch, France
    Brain 132:2688-98. 2009
    ..001, P = 0.008 and P = 0.01, respectively). The lack of pyramidal signs in most patients may be explained by masking due to severe motor neuropathy...
  16. ncbi Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells
    C Yoon
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    Development 124:3157-65. 1997
    ..Furthermore, they provide the basis for further studies on this novel RNA localization pattern and on germ-line development in general...
  17. ncbi Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Cell 119:381-92. 2004
    ..Plausibly, DDX3 is the human RNA helicase which functions in the CRM1 RNA export pathway analogously to the postulated role for Dbp5p in yeast mRNA export...
  18. pmc RNA helicases at work: binding and rearranging
    Eckhard Jankowsky
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Trends Biochem Sci 36:19-29. 2011
    b>RNA helicases are ubiquitous, highly conserved enzymes that participate in nearly all aspects of RNA metabolism. These proteins bind or remodel RNA or RNA-protein complexes in an ATP-dependent fashion...
  19. pmc The human VASA gene is specifically expressed in the germ cell lineage
    D H Castrillon
    Women s and Perinatal Pathology Division, Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:9585-90. 2000
    ..The gene we have isolated is thus a highly specific marker of germ cells and should be useful for studies of human germ cell determination and function...
  20. ncbi RNA chaperones, RNA annealers and RNA helicases
    Lukas Rajkowitsch
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    RNA Biol 4:118-30. 2007
    ..DExD/H-box containing RNA helicases are discussed as a special family of enzymes that restructure RNA or RNPs in an ATP-dependent manner...
  21. ncbi Senataxin, the ortholog of a yeast RNA helicase, is mutant in ataxia-ocular apraxia 2
    Maria Ceu Moreira
    IGBMC Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, ULP 67404 Illkirch, C U de Strasbourg, France
    Nat Genet 36:225-7. 2004
    ..Ten of the fifteen mutations cause premature termination of a large DEAxQ-box helicase, the human ortholog of yeast Sen1p, involved in RNA maturation and termination...
  22. pmc P body-associated protein Mov10 inhibits HIV-1 replication at multiple stages
    Ryan Burdick
    HIV Drug Resistance Program, National Cancer Institute Frederick, Viral Mutation Section, Frederick, MD 21702, USA
    J Virol 84:10241-53. 2010
    ..Overall, these results show that Mov10 can potently inhibit HIV-1 replication at multiple stages...
  23. pmc An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila
    Daniel Olivieri
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences IMBA, Vienna, Austria
    EMBO J 29:3301-17. 2010
    ..Loss of Zucchini leads to an accumulation of Piwi and Armitage in Yb bodies, indicating that Yb bodies are sites of primary piRNA biogenesis...
  24. ncbi Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa
    Vladimir Pena
    AG Röntgenkristallographie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Mol Cell 25:615-24. 2007
    ..We conclude that the expanded Prp8 Jab1/MPN domain represents a pseudoenzyme converted into a protein-protein interaction platform and that dysfunction of this platform underlies Retinitis pigmentosa...
  25. ncbi RNA helicases in infection and disease
    Lenz Steimer
    University of Muenster, Institute for Physical Chemistry, Muenster, Germany
    RNA Biol 9:751-71. 2012
    b>RNA helicases unwind their RNA substrates in an ATP-dependent reaction, and are central to all cellular processes involving RNA...
  26. pmc Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs
    A van Hoof
    Department of Molecular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA
    Mol Cell Biol 20:441-52. 2000
    ..This finding suggests that the cytoplasmic and nuclear forms of the exosome represent two functionally different complexes involved in distinct 3'-to-5' processing and degradation reactions...
  27. ncbi Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nature 437:1167-72. 2005
    ..within infected cells and is sensed by Toll-like receptor 3 (TLR3) and the recently identified cytosolic RNA helicases RIG-I (retinoic acid inducible gene I, also known as Ddx58) and Mda5 (melanoma differentiation-associated gene ..
  28. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage...
  29. pmc DExD/H-box RNA helicases in ribosome biogenesis
    Roman Martin
    Centre for Biochemistry and Molecular Cell Biology, Gottingen University, Gottingen, Germany
    RNA Biol 10:4-18. 2013
    Ribosome synthesis requires a multitude of cofactors, among them DExD/H-box RNA helicases. Bacterial RNA helicases involved in ribosome assembly are not essential, while eukaryotes strictly require multiple DExD/H-box proteins that are ..
  30. pmc Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation
    A N Kuhn
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, Wisconsin 53706 1532, USA
    Genetics 155:1667-82. 2000
    ....
  31. ncbi Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome
    Junjun Gao
    Institute for Cell and Molecular Biology, University of Texas, TX 78712, USA
    Mol Microbiol 61:394-406. 2006
    ..coli...
  32. pmc Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction
    Kay Childs
    Division of Basic Medical Sciences, St George s, University of London, London, United Kingdom
    J Virol 86:3411-21. 2012
    ..This is significant, because it demonstrates a general mechanism for the targeting of the RIG-I pathway by paramyxoviruses...
  33. ncbi Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes
    Christian Matranga
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cell 123:607-20. 2005
    ..For siRNAs, virtually all RISC is assembled through this cleavage-assisted mechanism. In contrast, passenger-strand cleavage is not important for the incorporation of miRNAs that derive from mismatched duplexes...
  34. pmc Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing
    Doris Ursic
    Laboratories of Molecular Biology and Genetics, R M Bock Laboratories, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
    Nucleic Acids Res 32:2441-52. 2004
    ..The protein-protein and protein-RNA interactions reported here suggest that the DNA/RNA helicase activity of Sen1p is utilized for several different purposes in multiple gene expression pathways...
  35. pmc DEAD-box proteins as RNA helicases and chaperones
    Inga Jarmoskaite
    Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA
    Wiley Interdiscip Rev RNA 2:135-52. 2011
    ..This review will describe the properties of DEAD-box proteins as RNA helicases and the current understanding of how the energy from ATPase activity is used to drive the separation of RNA ..
  36. pmc Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway
    Ke Zheng
    Center for Animal Transgenesis and Germ Cell Research and Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 107:11841-6. 2010
    ..The Mov10l1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins...
  37. pmc Perturbation of the P-body component Mov10 inhibits HIV-1 infectivity
    Vyacheslav Furtak
    Department of Microbiology, New York University School of Medicine, New York, New York, United States of America
    PLoS ONE 5:e9081. 2010
    ..Because Mov10 is implicated as part of the P-body complex, these findings point to the potential role of cytoplasmic RNA processing machinery in infectious retroviral production...
  38. ncbi NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity
    Hala Chamieh
    Equipe labelisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, Associée aux Universités Paris 6 et Paris 11, Avenue de la Terrasse, Gif sur Yvette, F 91198, France
    Nat Struct Mol Biol 15:85-93. 2008
    ..This work demonstrates that the EJC core is sufficient to stably anchor the UPF proteins to mRNA and provides insights into the regulation of its central effector, UPF1...
  39. ncbi VISA is an adapter protein required for virus-triggered IFN-beta signaling
    Liang Guo Xu
    Department of Immunology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, Colorado 80206, USA
    Mol Cell 19:727-40. 2005
    ..These findings suggest that VISA is critically involved in both virus-triggered TLR3-independent and TLR3-mediated antiviral IFN signaling...
  40. pmc Mechanism of induction and suppression of antiviral immunity directed by virus-derived small RNAs in Drosophila
    Roghiyh Aliyari
    Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA
    Cell Host Microbe 4:387-97. 2008
    ..Our findings, therefore, provide a model in which small RNA-directed viral immunity is induced during the initiation of viral progeny (+)RNA synthesis and suppressed by B2 inside the viral RNA replication complex...
  41. pmc RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Genes Dev 19:2176-86. 2005
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes...
  42. pmc Endogenous MOV10 inhibits the retrotransposition of endogenous retroelements but not the replication of exogenous retroviruses
    Shetal Arjan-Odedra
    Department of Infectious Diseases, King s College London School of Medicine, Borough Wing, Guy s Hospital, London Bridge, London, SE1 9RT, UK
    Retrovirology 9:53. 2012
    ..Here, we employ both ectopic expression and gene knockdown approaches to analyse the role of human MOV10 in the replication of a panel of exogenous retroviruses and endogenous retroelements...
  43. ncbi A DEAD-box RNA helicase in the Escherichia coli RNA degradosome
    B Py
    Nuffield Department of Clinical Biochemisstry and Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, UK
    Nature 381:169-72. 1996
    ..RhlB is a member of the DEAD-box family of ATP-dependent RNA helicases, which are found in both prokaryotes and eukaryotes...
  44. ncbi mRNA helicases: the tacticians of translational control
    Armen Parsyan
    Department of Biochemistry and Goodman Cancer Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec H3A 1A3, Canada
    Nat Rev Mol Cell Biol 12:235-45. 2011
    ....
  45. ncbi The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis
    N Kyle Tanner
    Departement de Biochimie Medicale, Centre Medical Universitaire, 1, rue Michel Servet, 1211 Geneva 4, Switzerland
    Mol Cell 11:127-38. 2003
    ..We have discovered a motif upstream of motif I that is unique to and characteristic of the DEAD box family of RNA helicases. It consists of a 9 amino acid sequence containing an invariant glutamine...
  46. ncbi Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities
    L Liang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Development 120:1201-11. 1994
    ..Initial recruitment of vasa to the pole plasm must consequently depend upon protein-protein interactions but, once localized, vasa must bind to RNA to mediate germ cell formation...
  47. pmc Moloney leukemia virus 10 (MOV10) protein inhibits retrovirus replication
    Xiaojun Wang
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    J Biol Chem 285:14346-55. 2010
    ..All of these results indicate that MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection...
  48. ncbi UPF1 is required for nonsense-mediated mRNA decay (NMD) and RNAi in Arabidopsis
    Luis Arciga-Reyes
    Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK
    Plant J 47:480-9. 2006
    ..Finally, gene silencing by an inverted repeat transgene is impaired in upf1-5 mutants, indicating a connection between UPF1 and RNA interference in plants...
  49. pmc Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I
    Yanli Wang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 17:781-7. 2010
    ....
  50. ncbi DExD/H box RNA helicases: from generic motors to specific dissociation functions
    N K Tanner
    , , rue Michel Servet, CH-1211 4, , Switzerland
    Mol Cell 8:251-62. 2001
    b>RNA helicases of the DEAD box and related DExD/H proteins form a very large superfamily of proteins conserved from bacteria and viruses to humans...
  51. pmc Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1
    Shang Yi Chiu
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, New York 14642, USA
    RNA 9:77-87. 2003
    ..These data indicate that hSmg5/7a targets protein phosphatase 2A to Upf1 but not Upf2. Results of Western blotting reveal that hSmg5/7a is mostly cytoplasmic in HEK293T cells...
  52. pmc The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs
    K Czaplinski
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School UMDNJ, USA
    Genes Dev 12:1665-77. 1998
    ....
  53. ncbi Functional role for senataxin, defective in ataxia oculomotor apraxia type 2, in transcriptional regulation
    Amila Suraweera
    Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    Hum Mol Genet 18:3384-96. 2009
    ..These data suggest that senataxin, similar to its yeast homolog Sen1p, plays a role in coordinating transcriptional events, in addition to its role in DNA repair...
  54. ncbi Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces cerevisiae, is an RNA helicase
    I Iost
    Departement de Biochimie Medicale, Centre Medical Universitaire, Universite de Geneve, 1211 Geneva 4, Switzerland
    J Biol Chem 274:17677-83. 1999
    ..Finally, RNA binding measurements indicate that the presence of ATP, but not ADP, increases the affinity of Ded1p for duplex versus single-stranded RNA; we discuss how this differential effect might drive the unwinding reaction...
  55. ncbi Domain structure of human nuclear DNA helicase II (RNA helicase A)
    S Zhang
    Abteilung Biochemie, Institut fur Molekulare Biotechnologie, Postfach 100813, D 07708 Jena, Germany
    J Biol Chem 272:11487-94. 1997
    ..A model is suggested in which the flanking domains influence and regulate the unwinding properties of NDH II...
  56. ncbi RNA helicases p68 and p72: multifunctional proteins with important implications for cancer development
    Frances V Fuller-Pace
    Centre for Oncology and Molecular Medicine, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK
    Future Oncol 7:239-51. 2011
    The DEAD box RNA helicases p68 (DDX5) and p72 (DDX17) play important roles in multiple cellular processes that are commonly dysregulated in cancers, including transcription, pre-mRNA processing/alternative splicing and miRNA processing...
  57. ncbi The BRCA1-interacting helicase BRIP1 is deficient in Fanconi anemia
    Orna Levran
    Laboratory for Human Genetics and Hematology, The Rockefeller University, New York, New York, USA
    Nat Genet 37:931-3. 2005
    ..Using genetic mapping, mutation identification and western-blot data, we identify the defective protein in FA-J cells as BRIP1 (also called BACH1), a DNA helicase that is a binding partner of the breast cancer tumor suppressor BRCA1...
  58. pmc RNA helicases: emerging roles in viral replication and the host innate response
    Arnaz Ranji
    Department of Veterinary Biosciences, Ohio State University, Columbus, OH, USA
    RNA Biol 7:775-87. 2010
    b>RNA helicases serve multiple roles at the virus-host interface...
  59. ncbi Evolutional conservation of molecular structure and antiviral function of a viral RNA receptor, LGP2, in Japanese flounder, Paralichthys olivaceus
    Maki Ohtani
    Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, South Korea
    J Immunol 185:7507-17. 2010
    ..These results suggest that Japanese flounder LGP2 plays an important role in the recognition of both viral ssRNA and dsRNA to induce the antiviral activity by the production of IFN-stimulated proteins...
  60. ncbi mRNA quality control: an ancient machinery recognizes and degrades mRNAs with nonsense codons
    Isabelle Behm-Ansmant
    MPI for Developmental Biology, Spemannstrasse 35, D 72076 Tubingen, Germany
    FEBS Lett 581:2845-53. 2007
    ..These studies are providing important insights that will aid the development of new treatments for at least some human genetic diseases...
  61. pmc Sorting of Drosophila small silencing RNAs
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 130:299-308. 2007
    ..Thus, in flies small-RNA duplexes are actively sorted into Argonaute-containing complexes according to their intrinsic structures...
  62. pmc Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex
    Christelle M Roux
    Department of Microbiology and Immunology, University of Rochester Medical Center, School of Medicine and Dentistry, Box 672, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Bacteriol 193:5520-6. 2011
    ..Results also revealed that the recently recognized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in other Gram-positive organisms...
  63. ncbi Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 54:1063-75. 2004
    ..In addition, we show that PNPase and RhlB within the degradosome cooperate to eliminate short degradation intermediates of ptsG mRNA...
  64. ncbi The BRCT domain is a phospho-protein binding domain
    Xiaochun Yu
    Department of Oncology, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Science 302:639-42. 2003
    ..Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control...
  65. ncbi Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'
    Annie Prud'homme-Généreux
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 54:1409-21. 2004
    Escherichia coli contains at least five ATP-dependent DEAD-box RNA helicases which may play important roles in macromolecular metabolism, especially in translation and mRNA decay...
  66. pmc Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila
    Astrid D Haase
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Genes Dev 24:2499-504. 2010
    ..Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis...
  67. pmc The hepatitis C virus NS3 protein: a model RNA helicase and potential drug target
    David N Frick
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Curr Issues Mol Biol 9:1-20. 2007
    ....
  68. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  69. ncbi Distinct pathways for snoRNA and mRNA termination
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 24:723-34. 2006
    ..These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation...
  70. pmc C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing
    Geurt Schilders
    Department of Biomolecular Chemistry, Nijmegen Center for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    Nucleic Acids Res 35:2564-72. 2007
    ..Taken together, our results are consistent with a role for the exosome-associated proteins C1D, MPP6 and KIAA052/hMtr4p in the recruitment of the exosome to pre-rRNA to mediate the 3' end processing of the 5.8S rRNA...
  71. ncbi Dynamics and processivity of 40S ribosome scanning on mRNA in yeast
    Karine Berthelot
    Department of Biomolecular Sciences, UMIST, Manchester, UK
    Mol Microbiol 51:987-1001. 2004
    ..We have developed a biased bidirectional walking model of ribosomal scanning that provides a framework for understanding the above observations as well as other known quantitative and qualitative features of this process...
  72. pmc MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs
    Robert J A Frost
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 107:11847-52. 2010
    ....
  73. pmc Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila
    Kuniaki Saito
    Keio University School of Medicine, Tokyo, Japan
    Genes Dev 24:2493-8. 2010
    ..Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing...
  74. ncbi The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes
    Eduard Nedea
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada
    Mol Cell 29:577-87. 2008
    ..Swd2 is also a subunit of the Set1c histone H3K4 methyltransferase complex and is required for its stability and optimal methyltransferase activity...
  75. pmc The p20 and Ded1 proteins have antagonistic roles in eIF4E-dependent translation in Saccharomyces cerevisiae
    J De la Cruz
    Departement de Biochimie Medicale, CMU, 1, rue Michel Servet, 1211 Geneve 4, Switzerland
    Proc Natl Acad Sci U S A 94:5201-6. 1997
    ..cerevisiae...
  76. pmc Fail-safe transcriptional termination for protein-coding genes in S. cerevisiae
    Ana G Rondon
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 36:88-98. 2009
    ..We propose that these alternative Pol II termination pathways serve the dual function of avoiding transcription interference and promoting rapid removal of aberrant transcripts...
  77. ncbi Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity
    Mitsutoshi Yoneyama
    Antiviral Innate Immunity Project, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Tokyo, Japan
    J Immunol 175:2851-8. 2005
    ..These results highlight ingenious mechanisms for initiating antiviral innate immune responses and the action of virus-encoded inhibitors...
  78. pmc Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I
    Rhea Sumpter
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    J Virol 79:2689-99. 2005
    ..RIG-I is thus a pathogen receptor that regulates cellular permissiveness to HCV replication and, as an interferon-responsive gene, may play a key role in interferon-based therapies for the treatment of HCV infection...
  79. pmc RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli
    Martin Lehnik-Habrink
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University Gottingen, Grisebachstr 8, D 37077 Gottingen, Germany
    J Bacteriol 193:5431-41. 2011
    ..In many bacteria, these RNases, as well as RNA helicases and other proteins, are organized in a protein complex called the RNA degradosome...
  80. pmc Differential involvement of the five RNA helicases in adaptation of Bacillus cereus ATCC 14579 to low growth temperatures
    Franck Pandiani
    INRA, UMR408, Sécurité et Qualité des Produits d Origine Végétale, Avignon, France
    Appl Environ Microbiol 76:6692-7. 2010
    ..The cold phenotype was restored with the cshA gene for the ΔcshA strain and partially for the ΔcshB strain but not for the ΔcshC strain, suggesting different functions at low temperature...
  81. ncbi Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1
    J Lykke-Andersen
    Howard Hughes Medical Institute, Molecular Biochemistry and Biophysics, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 293:1836-9. 2001
    ..Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of beta-globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance...
  82. ncbi RNA structural rearrangement via unwinding and annealing by the cyanobacterial RNA helicase, CrhR
    Danuta Chamot
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
    J Biol Chem 280:2036-44. 2005
    Rearrangement of RNA secondary structure is crucial for numerous biological processes. RNA helicases participate in these rearrangements through the unwinding of duplex RNA...
  83. ncbi The serine protease domain of hepatitis C viral NS3 activates RNA helicase activity by promoting the binding of RNA substrate
    Rudolf K F Beran
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 282:34913-20. 2007
    ....
  84. pmc Regulation of alternative splicing by the ATP-dependent DEAD-box RNA helicase p72
    Arnd Honig
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:5698-707. 2002
    Although a number of ATP-dependent RNA helicases are important for constitutive RNA splicing, no helicases have been implicated in alternative RNA splicing...
  85. ncbi RISC assembly defects in the Drosophila RNAi mutant armitage
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 116:831-41. 2004
    ..Our results suggest that armi is required for RISC maturation...
  86. pmc Sen1p performs two genetically separable functions in transcription and processing of U5 small nuclear RNA in Saccharomyces cerevisiae
    Jonathan S Finkel
    Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genetics 184:107-18. 2010
    ..The results suggest that the Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression...
  87. pmc Mechanism and specificity of a symmetrical benzimidazolephenylcarboxamide helicase inhibitor
    Craig A Belon
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Biochemistry 49:1822-32. 2010
    ..Results are discussed in light of a molecular model of a (BIP)(2)B-HCV helicase complex, which is unable to bind nucleic acid, thus preventing the enzyme from separating double-stranded nucleic acid...
  88. pmc Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2
    Darja Bamming
    Department of Biochemistry, Molecular Biology, and Cell Biology and Department of Medicine, Northwestern University, USA
    J Biol Chem 284:9700-12. 2009
    ..components of the cellular response to virus infection and are homologous to the DEXH box subfamily of RNA helicases. However, the relevance of helicase activity in the regulation of interferon production remains elusive...
  89. pmc Identification of molecular determinants from Moloney leukemia virus 10 homolog (MOV10) protein for virion packaging and anti-HIV-1 activity
    Aierken Abudu
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824 4320, USA
    J Biol Chem 287:1220-8. 2012
    ..Taken together, we have identified two critical MOV10 packaging determinants and eight other critical residues for anti-HIV-1 activity. These results provide a molecular basis for further understanding the MOV10 antiretroviral mechanism...
  90. ncbi Further characterization of the helicase activity of eIF4A. Substrate specificity
    G W Rogers
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4935, USA
    J Biol Chem 276:12598-608. 2001
    Eukaryotic initiation factor (eIF) 4A is the archetypal member of the DEAD box family of RNA helicases and is proposed to unwind structures in the 5'-untranslated region of mRNA to facilitate binding of the 40 S ribosomal subunit...
  91. pmc deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2
    W M Toone
    Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada
    J Bacteriol 173:3291-302. 1991
    ..We suggest that the DeaD protein plays a hitherto unknown role in translation in E. coli...
  92. pmc The Yb body, a major site for Piwi-associated RNA biogenesis and a gateway for Piwi expression and transport to the nucleus in somatic cells
    Hongying Qi
    Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511, USA
    J Biol Chem 286:3789-97. 2011
    ..Finally, we discovered putative endo-siRNAs in the flamenco locus and the Yb dependence of their expression. These observations further implicate a role for Yb in transposon silencing via both the piRNA and endo-siRNA pathways...
  93. ncbi Innate immune recognition of viral infection
    Taro Kawai
    Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Suita, Osaka 565 0871, Japan
    Nat Immunol 7:131-7. 2006
    ..Cells also express cytoplasmic RNA helicases that function as alternative pattern-recognition receptors through recognition of double-stranded RNA produced ..
  94. pmc The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:795-804. 2008
    ..Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3...
  95. ncbi Toll-like receptors and RNA helicases: two parallel ways to trigger antiviral responses
    Etienne Meylan
    Department of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Mol Cell 22:561-9. 2006
    ..Recently, a second system has been described, which relies on the cytoplasmic recognition of dsRNA by RNA helicases such as RIG-I...
  96. ncbi Truncating mutations in the Fanconi anemia J gene BRIP1 are low-penetrance breast cancer susceptibility alleles
    Sheila Seal
    Section of Cancer Genetics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
    Nat Genet 38:1239-41. 2006
    ..Thus, inactivating truncating mutations of BRIP1, similar to those in BRCA2, cause Fanconi anemia in biallelic carriers and confer susceptibility to breast cancer in monoallelic carriers...
  97. ncbi vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development
    S Styhler
    Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1, Canada
    Development 125:1569-78. 1998
    ..However, GRK accumulation in the oocyte is severely reduced in the absence of vasa function, suggesting a function for VASA in activating gurken translation in wild-type ovaries...
  98. ncbi Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases
    Thierry Bizebard
    Laboratoire de Biochimie et Enzymologie Structurales, CNRS UPR 9063, Bat 34, CNRS, 91198 Gif sur Yvette, Cedex, France
    Biochemistry 43:7857-66. 2004
    ..This behavior, which contrasts with that of well studied SF1 DNA helicases, is discussed in the light of available structural and biochemical data...
  99. pmc An RNA degradosome assembly in Caulobacter crescentus
    Steven W Hardwick
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
    Nucleic Acids Res 39:1449-59. 2011
    ..Finally, we observe that the abundance of RNase E varies through the cell cycle, with maxima at morphological differentiation and cell division. This variation may contribute to the program of gene expression during cell division...
  100. ncbi The ATPase, RNA unwinding, and RNA binding activities of recombinant p68 RNA helicase
    Youliang Huang
    Program in Cell and Molecular Biosciences, Department of Animal and Dairy Sciences, Auburn University, 210 Upchurch Hall, Auburn, Alabama 36849, USA
    J Biol Chem 277:12810-5. 2002
    ..Interestingly, the recombinant p68 RNA helicase unwinds dsRNA in both 3' --> 5' and 5' --> 3' directions. This is the second example of a Asp-Glu-Ala-Asp (DEAD) box RNA helicase that unwinds RNA duplexes in a bi-directional manner...
  101. ncbi The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification
    Heather A Cook
    Program in Molecular Medicine and the Program in Cell Dynamics, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Cell 116:817-29. 2004
    ..We conclude that RNA silencing is essential for establishment of the cytoskeletal polarity that initiates embryonic axis specification and for translational control of oskar mRNA...

Research Grants70

  1. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..either NTP or the 5'-triphosphorylated RNA and the recently reported crystal structures of YFV and DEN2 RNA helicases and those of hepatitis C virus and bovine viral diarrhea virus (BVDV) RdRP for docking studies...
  2. Structure and Function of the Hepatitis C Virus Genome
    Peter Sarnow; Fiscal Year: 2013
    ..To do this, novel biochemical, structural and genetic approaches will be employed to study protein-HCV RNA interactions. ..
  3. Structural Studies of RNA Maturation and Processing
    Christopher D Lima; Fiscal Year: 2013
    ..Pathways and enzymes that regulate the abundance, lifetime, and processing of RNA have been associated with human diseases that include neurodegenerative disorders, cancer, and inflammation. ..
  4. Antiviral potential of helicase inhibitors
    David N Frick; Fiscal Year: 2012
    ..We will also develop similar assays to identify compounds that might inhibit viruses that cause AIDS, yellow fever, Dengue fever, West Nile and Japanese encephalitis. ..
  5. Novel Compounds to Inactivate Oncogenic Fusion Proteins
    Jeffrey A Toretsky; Fiscal Year: 2013
    ..This project will specifically address the need for new targeted therapies for ESFT and a large number of related malignancies that have chromosomal translocations. ..
  6. Characterization of a novel role for DEAD-box RNA helicases in bacteria.
    CHRISTOPHER ANTHONY VAKULSKAS; Fiscal Year: 2013
    ..The proposed studies detail a plan to investigate the role of DEAD-box RNA helicases in Csr regulation...
  7. Functions of nuclear non-canonical poly(A) polymerases in Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2010
    ..a specific complex with ncPAP1;and 2) transcripts polyadenylated by ncPAP1 are targeted to the exosome via RNA helicases. PUBLIC HEALTH RELEVANCE: Trypanosomatids are the causative agents of major parasitic diseases in developing ..
  8. Neuronal RNA processing defects in ALS4 caused by SETX mutations
    Craig L Bennett; Fiscal Year: 2013
    ..This underscores the importance of senataxin function in the survival of various neuronal populations. Our studies seek to determine the molecular mechanisms leading to motor neuron loss in ALS4 and further characterize the ..
  9. Hepatitis C and Tumor Suppressors in Hepatocellular
    Stanley M Lemon; Fiscal Year: 2011
    ..performance site ..
  10. Mucosal innate immune defense to Rift Valley fever virus
    Amy G Hise; Fiscal Year: 2010
    ..Additionally, the research tools that we develop, including a murine inhalation model will have wider applicability to the study of viruses transmitted via a respiratory route. ..
  11. Circadian Programs in Bacteria
    Carl Hirschie Johnson; Fiscal Year: 2013
    ..Knowledge of circadian mechanisms along with the development of therapies to properly phase sleep will allow us to enhance health, performance, and well-being in addition to improving the quality of life for depressed subjects. ..
  12. Analyses of two RNA helicases that affect intercellular transport via plasmodesma
    Patricia C Zambryski; Fiscal Year: 2012
    ..ISE1 and ISE2 encode two distinct types of RNA helicases with independent roles in PD mediated transport...
  13. Roles of RNA helicase and ribosomal biogenesis in normal and diseased muscle
    Vandana Gupta; Fiscal Year: 2013
    ..DEAD-box RNA helicases are involved in all cellular processes involving RNA, from transcription, mRNA splicing, translation, RNA ..
  14. Innate Immune Sensing of Rift Valley Fever Virus
    Amy G Hise; Fiscal Year: 2010
    ..acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (Mda-5)/Helicard), two DExD/H box RNA helicases contain protein interaction caspase recruitment and activation domains (CARD) and recognize specific viral ..
  15. Regulation of RNA Surveillance
    MILES FROME WILKINSON; Fiscal Year: 2012
    ..This proposal addresses newly discovered mechanisms responsible for regulating NMD that are particularly relevant for understanding normal neurological function and the genetic basis for mental retardation. ..
  16. The functional role of DRH-1 in RNAi directed viral immunity
    Rui Lu; Fiscal Year: 2013
    ..Increasing evidence suggests that putative RNA helicases also play important roles in RDVI across kingdoms...
  17. Translation Regulation of Collagen-1 in Liver Fibrosis: Target of Drug Discovery
    Zarko Manojlovic; Fiscal Year: 2013
    ....
  18. MicroRNAs, cardiac development and function
    Da Zhi Wang; Fiscal Year: 2013
    ..Aim #2. To investigate the molecular events mediated by Csm, a cardiac-specific RNA helicase, in miRNA pathway. Aim #3. To define the role of Csm in cardiac development and cardiomyocyte proliferation. ..
  19. REPLICATION OF INFLUENZA VIRUS
    Robert M Krug; Fiscal Year: 2013
    ..Two cellular RNA helicases, DHX30 and DDX21, are also associated with these complexes...
  20. Assays for Discovery of Translation Inhibitors
    Gerhard Wagner; Fiscal Year: 2009
    ..The proposed study will allow us to obtain leads for anti-cancer and anti-viral drug design. ..
  21. Vertebrate Non-Coding RNPs: Integrating function, biogenesis and survival
    Joan A Steitz; Fiscal Year: 2013
    ..Together, this understanding will contribute to the development of therapeutic approaches to combat multiple genetic diseases that derive from faulty RNA processing and surveillance, as well as cancer. ..
  22. Regulation of Type I Collagen in Hepatic Fibrosis
    Branko Stefanovic; Fiscal Year: 2012
    ..The results will elucidate this unique mechanism of collagen synthesis in HSCs and identify the regulatory roles of the factors involved, as potential targets for development of antifibrotic drugs. ..
  23. The Role of DEAD-box Proteins in Gene Expression
    Elizabeth J Tran; Fiscal Year: 2013
    ..Central to this process, are a class of RNA helicases called DEAD-box proteins that perform essential roles in all aspects of RNA biology...
  24. Cardiac specific RNA Helicase is critical for normal cardiomyocyte proliferation
    RONALD LEE NEPPL; Fiscal Year: 2012
    ..DEAD-box RNA helicases are involved in virtually all biological processes involving RNA;specifically the nuclear DEAD-box RNA ..
  25. Protein Arginine Methylation in Trypanosomes
    Laurie K Read; Fiscal Year: 2013
    ..In addition, because the effects of arginine methylation on RNA metabolism are not well understood in general, they will also provide insight into a common posttranslational modification in higher eukaryotes. ..
  26. Mechanisms of Viral DNA Packaging: Biophysical, Biochemical, &Genetic Analysis
    Douglas E Smith; Fiscal Year: 2013
    ..studies on other virus systems and help to define general principles of ATP-driven molecular motors relevant to understanding homologous cellular complexes including RNA helicases and chromosome segregation factors.
  27. Mechanism of CsrA-Mediated Global Control
    Tony Romeo; Fiscal Year: 2013
    ..Thus, these studies will provide fundamental understanding of the regulation of bacterial metabolism and pathogenesis and may suggest novel therapeutic and/or vaccine strategies. ..
  28. Regulation of AU-rich element-mediated mRNA decay
    Ching Yi Chen; Fiscal Year: 2013
    ..Understanding the regulation of mRNA degradation would provide insights into the disease pathogenesis. Our research should allow for the development of alternative treatments for immune and inflammatory diseases and cancer. ..
  29. Functional characterization of piRNPs
    Zissimos Mourelatos; Fiscal Year: 2013
    ..In turn, this knowledge will lead to important insights towards understanding stem cell biology and diseases that affect reproduction and development. ..
  30. The role of CshA and CshB in selective mRNA protection in S. aureus
    AMBROSE LIN YAU CHEUNG; Fiscal Year: 2013
    ..CshA and CshB are potential DEAD-box RNA helicases while SA1641 has homology with RNA methyltransferase...
  31. Innate Immunity and Herpes Simplex Infection
    ROBERT WILLIAM FINBERG; Fiscal Year: 2010
    ..All Projects involve the use of common reagents and the definition of new paradigms related to recognition and response of the host to HSV. ..
  32. Senataxin mutations in familial motor neuron disease (ALS4) and Ataxia (AOA2)
    Gwenn A Garden; Fiscal Year: 2010
    ..Since there is currently no cure or preventive treatment for these conditions, any knowledge gained has the potential to provide future diagnostic and therapeutic interventions for these patients. ..
  33. Functions of MOV10L1 in piRNA biogenesis and germ cell development
    Peijing Jeremy Wang; Fiscal Year: 2013
    ..These studies will provide insight into etiology, diagnosis, and prevention of human diseases including male infertility and birth defects. ..
  34. Protein Arginine Methylation in Trypanosomes
    LAURIE READ; Fiscal Year: 2009
    ..They will also provide insight into novel functions of a common posttranslational modification in higher eukaryotes. ..
  35. Physical and functional probing of DEAD-box proteins as general RNA chaperones
    Rick Russell; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): DEAD-box proteins are superfamily 2 RNA helicases that are required for virtually every process carried out by structured RNAs, from pre-mRNA splicing and translation to intracellular trafficking of ..
  36. Role of PISA;a PYHIN Family Member in Intracellular DNA Recognition
    Eicke Latz; Fiscal Year: 2013
    ..RNA is sensed via Toll-like receptor (TLR)-3, -7 and -8 or by the cytoplasmic RNA helicases RIG-I and MDA-5...
  37. Cellular responses to human metapneumovirus infection
    Xiaoyong Bao; Fiscal Year: 2011
    ..I have recently shown that the RNA helicases-MAVS signaling pathway plays a significant role in hMPV-induced expression of immune and inflammatory genes, ..
  38. The mechanisms of eukaryotic translation termination and ribosomal recycling
    Tatyana V Pestova; Fiscal Year: 2013
    ....
  39. An unexpected role for elF4a3 in regulating GPx1 selenoprotein expression
    MICHAEL EVEREST BUDIMAN; Fiscal Year: 2010
    ....
  40. Isolation and small molecule targeting of Ewing's Sarcoma stem cells
    Jeffrey A Toretsky; Fiscal Year: 2012
    ....
  41. Fluorescent Nucleosides and Oligonucleotides
    Yitzhak Tor; Fiscal Year: 2013
    ..will facilitate: (i) The discovery of new antibiotics targeting the bacterial ribosome (ii) The study of RNA helicases, ubiquitous motor proteins, which are involved in nearly every aspect of RNA metabolism, (iii) The monitoring ..
  42. Biogenesis and Function of Guide RNAs
    RUSLAN AFASIZHEV; Fiscal Year: 2012
    ..In preliminary studies we identified GRBC1/2-associated proteins, including NUDIX hydrolases, DExD/H RNA helicases, RNA binding proteins, and polypeptides lacking identifiable domains...
  43. Evaluation of Novel DExD/H Helicases in Innate Immune Signaling
    Glen N Barber; Fiscal Year: 2013
    ..using a yeast two-hybrid screen for FADD-interacting proteins, we have isolated two novel DexD/H box RNA helicases. The first, referred to as DDX-I, was isolated through a two hybrid screen using IPS-1 as a bait...
  44. YK-4-279 specifically targets ETS family fusion-protein cancers in clinical trial
    Jeffrey A Toretsky; Fiscal Year: 2010
    ..Future growth will also arise from the targeting of additional cancers and other diseases that rely on aberrant transcription to improve human health. ..
  45. Helicase Regulation of dsRNA Signaling and Innate Antiviral Immune Responses
    Curt M Horvath; Fiscal Year: 2012
    ..This strategy will reveal the strengths and vulnerabilities of the antiviral response and identify targets for therapeutic intervention. ..
  46. Role of the SrmB Helicase in Ribosome Assembly investigated by Mass Spectrometry
    Carla F Cervantes; Fiscal Year: 2013
    ..studies proposed here will lead to a deeper and more detailed understanding of the molecular mechanism whereby RNA helicases operate on their targets as well as the complex process of ribosome biogenesis, which is paramount for the ..
  47. Transcriptional and Posttranscriptional Regulation of Neurospora Circadian Clock
    Yi Liu; Fiscal Year: 2013
    ..Because of the similarities between the Neurospora and animal clocks, our results will provide important information on how eukaryotic circadian clocks function. ..
  48. Initiation and Regulation of Antiviral Innate Immunity
    Jonathan C Kagan; Fiscal Year: 2013
    ..By focusing on the earliest triggers of immune activation (the detection of viruses that enter our cells) we hope to uncover antiviral defense strategies that might be applicable to fight all infections. ..
  49. The Control of Gene Expression by Eukaryotic Ribonucleases
    Guillaume F Chanfreau; Fiscal Year: 2013
    ..Our studies of the turnover of unspliced and mis-spliced RNAs will shed light on the mechanisms by which RNA degradation controls the expression of genes mutated in the context of a large number of genetic diseases. ..
  50. Comparative Analysis of RNA Helicases in E. coli
    Chaitanya Jain; Fiscal Year: 2012
    DEAD-box RNA helicases are a versatile class of conserved proteins characterized by their ability to unwind RNA duplexes, which are believed to play important roles in regulating RNA function...
  51. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2013
    ..Because of the similarities between the Neurospora and animal clocks, our results will provide important information on how eukaryotic circadian clock functions. ..
  52. Innate Immune Response to Human Metapneumovirus Infection
    Antonella Casola; Fiscal Year: 2013
    ..Furthermore, the results obtained from these studies will be instrumental for the development of safer and more effective hMPV vaccines. ..
  53. The Functions of the DEAD-Box RNA Helicase Has1 in 60S Ribosome Biogenesis
    JILL ANN DEMBOWSKI; Fiscal Year: 2012
    ..These factors include exo- and endonucleases, GTP- and ATPases, RNA helicases, and RNA binding and scaffolding proteins...
  54. The FRH DEXH box helicase: analysis of a core component of the Neurospora circadi
    Jennifer Hurley; Fiscal Year: 2012
    ..Since the Neurospora clock is often used to understand the human clock, this could give us greater insight into our cycle and all of the implications that are associated with it. ..
  55. Alternative mechanisms of different stages in eukaryotic translation
    ANDREY PISAREV; Fiscal Year: 2013
    ....
  56. Picornavirus-initiated inflamation: novel parallels from Drosophila
    Siddharth Balachandran; Fiscal Year: 2013
    ..Cardiomyocytes detect the presence of a picornavirus in the cytoplasm via RNA helicases called RLRs (RIG-I-like receptors)...
  57. Mechanisms of VSV-mediated Oncolysis
    Glen N Barber; Fiscal Year: 2010
    ..However, it has recently been reported that the DExD/H box RNA helicases, RIG-I (Retinoic acid inducible gene-I) and MDA5 (melanoma differentiation antigen 5) are key players in ..
  58. Targeting the DHX9 Helicase for Small Molecule Probe Discovery
    Jerry Pelletier; Fiscal Year: 2013
    ..A set of cell-based assays will be developed to evaluate the effects of small molecule inhibitors on DHX9- dependent translation and on synthetic lethal relationships for triggering cancer cell death. ..
  59. MOLECULAR ANALYSIS OF TRANSCRIPTIONAL REPRESSION
    David N Arnosti; Fiscal Year: 2013
    ..The insights gained from these studies will provide essential underpinnings for population and evolutionary studies of development and disease in higher eukaryotes. ..
  60. HIV-1 capsid assembly intermediates: cellular factors and links to pathogenesis
    Jaisri R Lingappa; Fiscal Year: 2013
    ....
  61. RNA-binding RNA Polymerase II CTD-associated Proteins
    Jeffry L Corden; Fiscal Year: 2013
    ....
  62. Structure and Function of the Hepatitis C Virus Genome
    PETER contact SARNOW; Fiscal Year: 2010
    ..To do this, novel biochemical, structural and genetic approaches will be employed to study protein-HCV RNA interactions. ..
  63. FUNCTIONAL ANALYSIS OF DENGUE VIRUS ANTIGENS NS3 AND NS5
    Radhakrishnan Padmanabhan; Fiscal Year: 2003
    ..4. NS3/NS5 interaction is regulated by phosphorylation and subcellular location of NS5. The PI proposes to test the hypothesis that the nuclear NS5 regulates the expression of cellular gene(s) in response to viral infection. ..
  64. STRUCTURE BASED ANALYSIS OF DENGUE VIRUS SERINE PROTEASE
    Krishna Murthy; Fiscal Year: 2004
    ..It will also permit a structural definition of alternate targets for inhibition of the protease and their detailed evaluation. ..
  65. "Analyses of two genes that affect plasmodesmata structure and function"
    Patricia Zambryski; Fiscal Year: 2007
    ..ISE1 and ISE2 encode two different types of RNA helicases. ISE1 localizes to PD, and ISE2 localizes to cytoplasmic granules resembling RNA processing bodies...
  66. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..The overall findings are expected to improve our ability to exploit RNAi as an experimental and/or therapeutic tool, with likely impacts in both medicine and agriculture. ..
  67. Helicases: Structure, Function, & Roles in Human Disease
    STEVEN MATSON; Fiscal Year: 2001
    ..function and mechanism; DNA helicases in DNA replication, DNA helicases in DNA repair and recombination, RNA helicases in transcription, splicing, RNA stability and transport, RNA helicases in translation and ribosome hiegenesis; ..
  68. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..Specific Aim 6. Determine X-ray structures of PABP recognizing poly (A) RNA, and various binary and ternary complexes with a PABP-interacting protein and eIF4G. ..
  69. FUNCTIONAL SPLICEOSOME AND EXTRINSIC SPLICING FACTORS
    Ren Jang Lin; Fiscal Year: 2006
    ..We will analyze the splicing machinery when DBP2 function is impaired in vivo or in vitro. The proposed study shall reveal the dynamic features and the remodeling of the spliceosome core in yeast and human. ..
  70. CELLULAR FACTORS INVOLVED IN NUCLEAR EXPORT OF VIRAL RNA
    Flossie Wong Staal; Fiscal Year: 2001
    ....