dna polymerase beta

Summary

Summary: A DNA repair enzyme that catalyzes DNA synthesis during base excision DNA repair. EC 2.7.7.7.

Top Publications

  1. pmc DNA polymerase structure-based insight on the mutagenic properties of 8-oxoguanine
    William A Beard
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mutat Res 703:18-23. 2010
  2. pmc DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding
    Nisha A Cavanaugh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    J Biol Chem 285:24457-65. 2010
  3. pmc DNA base excision repair: a mechanism of trinucleotide repeat expansion
    Yuan Liu
    Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA
    Trends Biochem Sci 37:162-72. 2012
  4. pmc XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage
    M Masson
    UPR 9003 du Centre National de la Recherche Scientifique, Cancerogenese et Mutagenese Moleculaire et Structurale, Ecole Superieure de Biotechnologie de Strasbourg, 67400 Illkirch Graffenstaden, France
    Mol Cell Biol 18:3563-71. 1998
  5. pmc Polymerase mu is a DNA-directed DNA/RNA polymerase
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:2309-15. 2003
  6. ncbi Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III
    Meindert H Lamers
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Cell 126:881-92. 2006
  7. ncbi SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification
    G J McKenzie
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    Mol Cell 7:571-9. 2001
  8. ncbi DNA joint dependence of pol X family polymerase action in nonhomologous end joining
    James M Daley
    Graduate Program in Cellular and Molecular Biology and Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 0602, USA
    J Biol Chem 280:29030-7. 2005
  9. pmc Involvement of Escherichia coli DNA polymerase IV in tolerance of cytotoxic alkylating DNA lesions in vivo
    Ivana Bjedov
    INSERM U571, Faculte de Medecine, Universite Paris 5, 75730 Paris Cedex 15, France
    Genetics 176:1431-40. 2007
  10. ncbi AP endonuclease-independent DNA base excision repair in human cells
    Lee Wiederhold
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA
    Mol Cell 15:209-20. 2004

Research Grants

  1. MECHANISM OF BASE EXCISION REPAIR IN HIGHER EUKARYOTES
    Yoshihiro Matsumoto; Fiscal Year: 2003
  2. DNA Polymerase Beta and Gastric Cancer
    DAWIT KIDANE MULAT; Fiscal Year: 2013
  3. Base Excision Repair and Autoimmunity
    Joann B Sweasy; Fiscal Year: 2013
  4. Characterization of a DNA polymerase Beta deficient mouse
    Joann B Sweasy; Fiscal Year: 2013
  5. DNA POLYMERASE BETA AND MUTAGENESIS
    Joann B Sweasy; Fiscal Year: 2013
  6. MOLECULAR BASIS OF VIRAL AND CELLULAR TRANSFORMATION
    DANIEL C DIMAIO; Fiscal Year: 2013
  7. DNA Polymerase Fidelity Mechanisms: Theory and Experiment
    MYRON GOODMAN; Fiscal Year: 2012
  8. DNA Polymerase Beta Variants and Cancer
    Joann B Sweasy; Fiscal Year: 2013
  9. HYDROXYL RADICAL BIOLOGY BY CEREBRAL ISCHEMIA
    Philip Liu; Fiscal Year: 1999
  10. Dynamic Characterization of Cancer-Associated Variants of DNA Polymerase Beta
    ELIZABETH MARGARET MOSCATO-GOODPASTER; Fiscal Year: 2013

Detail Information

Publications281 found, 100 shown here

  1. pmc DNA polymerase structure-based insight on the mutagenic properties of 8-oxoguanine
    William A Beard
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mutat Res 703:18-23. 2010
    ..In contrast, the binding pocket for the incoming nucleotide does not have this flexibility so that 8-oxodGTP insertion opposite cytosine is strongly discouraged...
  2. pmc DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding
    Nisha A Cavanaugh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    J Biol Chem 285:24457-65. 2010
    ..Here, we investigate the ability of DNA polymerase beta to utilize nucleotides with modified sugars...
  3. pmc DNA base excision repair: a mechanism of trinucleotide repeat expansion
    Yuan Liu
    Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA
    Trends Biochem Sci 37:162-72. 2012
    ....
  4. pmc XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage
    M Masson
    UPR 9003 du Centre National de la Recherche Scientifique, Cancerogenese et Mutagenese Moleculaire et Structurale, Ecole Superieure de Biotechnologie de Strasbourg, 67400 Illkirch Graffenstaden, France
    Mol Cell Biol 18:3563-71. 1998
    ..Given that XRCC1 is also associated with DNA ligase III via a second BRCT module and with DNA polymerase beta, our results provide strong evidence that PARP is a member of a BER multiprotein complex involved in the ..
  5. pmc Polymerase mu is a DNA-directed DNA/RNA polymerase
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:2309-15. 2003
    ....
  6. ncbi Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III
    Meindert H Lamers
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology and Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Cell 126:881-92. 2006
    ..Pol III (residues 1-917), reveals a unique chain fold with localized similarity in the catalytic domain to DNA polymerase beta and related nucleotidyltransferases...
  7. ncbi SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification
    G J McKenzie
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    Mol Cell 7:571-9. 2001
    ..We show that pol IV, together with the major replicase, pol III, can account for all adaptive point mutations at lac. The results identify a role for pol IV in inducible genetic change...
  8. ncbi DNA joint dependence of pol X family polymerase action in nonhomologous end joining
    James M Daley
    Graduate Program in Cellular and Molecular Biology and Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 0602, USA
    J Biol Chem 280:29030-7. 2005
    ..Finally, mammalian Pol X polymerases were able to differentially complement a pol4 mutation depending on the joint structure, demonstrating that these polymerases can participate in yeast NHEJ but with distinct properties...
  9. pmc Involvement of Escherichia coli DNA polymerase IV in tolerance of cytotoxic alkylating DNA lesions in vivo
    Ivana Bjedov
    INSERM U571, Faculte de Medecine, Universite Paris 5, 75730 Paris Cedex 15, France
    Genetics 176:1431-40. 2007
    ..This activity is of major biological relevance because alkylating agents are continuously produced endogenously in all living cells and are also present in the environment...
  10. ncbi AP endonuclease-independent DNA base excision repair in human cells
    Lee Wiederhold
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA
    Mol Cell 15:209-20. 2004
    ..NEIL1 stably interacts with other BER proteins, DNA polymerase beta (pol beta) and DNA ligase IIIalpha...
  11. pmc Magnesium-cationic dummy atom molecules enhance representation of DNA polymerase beta in molecular dynamics simulations: improved accuracy in studies of structural features and mutational effects
    Peter Oelschlaeger
    University of Southern California, Department of Chemistry, Los Angeles, CA 90089 1062, USA
    J Mol Biol 366:687-701. 2007
    Human DNA polymerase beta (pol beta) fills gaps in DNA as part of base excision DNA repair. Due to its small size it is a convenient model enzyme for other DNA polymerases...
  12. pmc Escherichia coli DNA polymerase IV mutator activity: genetic requirements and mutational specificity
    J Wagner
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya Ku, Tokyo 158 8501, Japan
    J Bacteriol 182:4587-95. 2000
    ..These results are discussed with respect to the recently described biochemical characteristics of DNA Pol IV...
  13. pmc Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center, Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 37:3431-41. 2009
    b>DNA polymerase beta (Pol beta) is a key enzyme in DNA base excision repair, and an important factor for maintaining genome integrity and stability...
  14. ncbi The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis
    J Wagner
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan
    Mol Cell 4:281-6. 1999
    ..Along with the sequence homologies previously found within the UmuC-like protein family, these results indicate that the uncovered DNA polymerase activity may be a common feature of all these homologous proteins...
  15. pmc Coordination between polymerase beta and FEN1 can modulate CAG repeat expansion
    Yuan Liu
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 284:28352-66. 2009
    ..This is the first report illustrating that disruption of pol beta and FEN1 coordination during long-patch BER results in CAG repeat expansion...
  16. ncbi Nonoverlapping functions of DNA polymerases mu, lambda, and terminal deoxynucleotidyltransferase during immunoglobulin V(D)J recombination in vivo
    Barbara Bertocci
    INSERM U783, Faculté de Médecine René Descartes, Site Necker Enfants Malades, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
    Immunity 25:31-41. 2006
    ....
  17. pmc In situ analysis of repair processes for oxidative DNA damage in mammalian cells
    Li Lan
    Department of Molecular Genetics, Institute of Development, Aging, and Cancer, Tohoku University, Seiryomachi 4 1, Sendai 980 8575, Japan
    Proc Natl Acad Sci U S A 101:13738-43. 2004
    ..Furthermore, we characterized repair pathways by using repair-defective mammalian cells and found that DNA polymerase beta accumulated at single-strand breaks and oxidative base damage by means of its 31- and 8-kDa domains, ..
  18. pmc Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining
    Kihoon Lee
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Genetics 176:2003-14. 2007
    ..Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tel1 in MMEJ and genome integrity...
  19. ncbi Misalignment-mediated DNA polymerase beta mutations: comparison of microsatellite and frame-shift error rates using a forward mutation assay
    Kristin A Eckert
    Department of Pathology, Gittlen Cancer Research Institute, The Pennsylvania State University College of Medicine, Hershey Medical Center, 500 University Drive, Hershey, Pennsylvania 17033, USA
    Biochemistry 41:10490-8. 2002
    ..To our knowledge, this is the first direct quantitation of DNA polymerase errors in vitro using template microsatellite sequences...
  20. pmc A germline polymorphism of DNA polymerase beta induces genomic instability and cellular transformation
    Jennifer Yamtich
    Departments of Therapeutic Radiology and Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America
    PLoS Genet 8:e1003052. 2012
    ..Our results suggest that people who carry the rs3136797 germline SNP may be at an increased risk for cancer susceptibility...
  21. pmc Y265C DNA polymerase beta knockin mice survive past birth and accumulate base excision repair intermediate substrates
    Alireza G Senejani
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 109:6632-7. 2012
    ..DNA polymerase β is known to be a key player in the base excision repair pathway, and mice devoid of DNA polymerase beta do not live beyond a few hours after birth...
  22. pmc Differing conformational pathways before and after chemistry for insertion of dATP versus dCTP opposite 8-oxoG in DNA polymerase beta
    Yanli Wang
    Department of Chemistry and Courant Institute of Mathematical Sciences, New York University, New York, New York, USA
    Biophys J 92:3063-70. 2007
    To elucidate how human DNA polymerase beta (pol beta) discriminates dATP from dCTP when processing 8-oxoguanine (8-oxoG), we analyze a series of dynamics simulations before and after the chemical step with dATP and dCTP opposite an 8-..
  23. pmc In silico evidence for DNA polymerase-beta's substrate-induced conformational change
    Karunesh Arora
    Department of Chemistry and Courant Institute of Mathematical Sciences, New York University, New York, New York 10012, USA
    Biophys J 87:3088-99. 2004
    ..Further details gleaned from essential dynamics analyses clarify functionally relevant global motions of the polymerase-beta/DNA complex as required to prepare the system for the chemical reaction of nucleotide extension...
  24. pmc The Leu22Pro tumor-associated variant of DNA polymerase beta is dRP lyase deficient
    Shibani Dalal
    Department of Therapeutic Radiology and Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nucleic Acids Res 36:411-22. 2008
    Approximately 30% of human tumors characterized to date express DNA polymerase beta (pol beta) variant proteins...
  25. pmc Incorrect nucleotide insertion at the active site of a G:A mismatch catalyzed by DNA polymerase beta
    Ping Lin
    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 105:5670-4. 2008
    Based on a recent ternary complex crystal structure of human DNA polymerase beta with a G:A mismatch in the active site, we carried out a theoretical investigation of the catalytic mechanism of incorrect nucleotide incorporation using ..
  26. pmc Self-synthesizing DNA transposons in eukaryotes
    Vladimir V Kapitonov
    Genetic Information Research Institute, 1925 Landings Drive, Mountain View, CA 94043, USA
    Proc Natl Acad Sci U S A 103:4540-5. 2006
    ....
  27. pmc Sequential side-chain residue motions transform the binary into the ternary state of DNA polymerase lambda
    Meredith C Foley
    Department of Chemistry and Courant Institute of Mathematical Sciences, New York University, New York, New York, USA
    Biophys J 91:3182-95. 2006
    ..Our combined studies suggest that while pol lambda does not demonstrate large-scale subdomain movements as DNA polymerase beta (pol beta), significant DNA motion exists, and there are sequential subtle side chain and other motions-..
  28. ncbi Influence of DNA structure on DNA polymerase beta active site function: extension of mutagenic DNA intermediates
    William A Beard
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:31921-9. 2004
    ....
  29. pmc Stoichiometry of base excision repair proteins correlates with increased somatic CAG instability in striatum over cerebellum in Huntington's disease transgenic mice
    Agathi Vassiliki Goula
    Department of Neurobiology and Genetics, Institute of Genetics and Molecular and Cellular Biology, UMR 7104 CNRS INSERM UdS, Illkirch, France
    PLoS Genet 5:e1000749. 2009
    ..We propose that the stoichiometry of BER enzymes is one critical factor underlying the tissue selectivity of somatic CAG expansion...
  30. pmc DNA polymerase beta is critical for mouse meiotic synapsis
    Dawit Kidane
    Department of Therapeutic Radiology, The Yale Comprehensive Cancer Center, New Haven, CT, USA
    EMBO J 29:410-23. 2010
    We have shown earlier that DNA polymerase beta (Pol beta) localizes to the synaptonemal complex (SC) during Prophase I of meiosis in mice...
  31. pmc A novel mechanism of sugar selection utilized by a human X-family DNA polymerase
    Jessica A Brown
    Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 395:282-90. 2010
    ..Such steric clash was further demonstrated to be dependent on the size and orientation of the substituent covalently attached at the ribonucleotide C2'-position...
  32. ncbi DNA polymerases beta and lambda bypass thymine glycol in gapped DNA structures
    Ekaterina A Belousova
    Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia
    Biochemistry 49:4695-704. 2010
    ..fidelity of Tg bypass and does not influence normal DNA synthesis catalyzed by DNA polymerase lambda, (iv) DNA polymerase beta catalyzes the incorporation of all four dNTPs opposite Tg, and (v) hPCNA as well as hRPA has no specific ..
  33. pmc Control of DNA polymerase lambda stability by phosphorylation and ubiquitination during the cell cycle
    Ursula Wimmer
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Switzerland
    EMBO Rep 9:1027-33. 2008
    ..In particular, Pol lambda is stabilized during cell cycle progression in the late S and G2 phases. This most likely allows Pol lambda to correctly conduct repair of damaged DNA during and after S phase...
  34. ncbi Modulation of DNA polymerase beta-dependent base excision repair in cultured human cells after low dose exposure to arsenite
    Peter Sykora
    Centre for Cellular and Molecular Biology, School of Biological Sciences, Deakin University, Australia
    Toxicol Appl Pharmacol 228:385-94. 2008
    ..To address this question we have investigated the regulation of DNA polymerase beta (Pol beta) and AP endonuclease (APE1), in response to low, physiologically relevant doses of arsenic...
  35. pmc Mechanism of error-free and semitargeted mutagenic bypass of an aromatic amine lesion by Y-family polymerase Dpo4
    Olga Rechkoblit
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 17:379-88. 2010
    ..Further extension leads to semitargeted mutations via this proposed polymerase-guided mechanism...
  36. pmc Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta
    F Boudsocq
    Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, MD 20892 2725, USA
    Nucleic Acids Res 29:4607-16. 2001
    ..Thus, although phylogenetically related to DinB polymerases, our studies suggest that the archaeal Dpo4 enzyme exhibits lesion-bypass properties that are, in fact, more akin to those of eukaryotic poleta...
  37. pmc The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair
    Agnieszka Gembka
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057, Zurich, Switzerland
    Nucleic Acids Res 35:2596-608. 2007
    ..and functionally interacts with several components of the base excision repair (BER) machinery namely DNA polymerase beta (Pol beta), flap endonuclease 1 (Fen 1), DNA ligase I (Lig I) and the MutY homologue of Schizosaccharomyces ..
  38. pmc An 8-oxo-guanine repair pathway coordinated by MUTYH glycosylase and DNA polymerase lambda
    Barbara van Loon
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Proc Natl Acad Sci U S A 106:18201-6. 2009
    ..These results reveal a cellular response pathway to ROS, important to sustain genomic stability and modulate carcinogenesis...
  39. ncbi Investigating the role of the little finger domain of Y-family DNA polymerases in low fidelity synthesis and translesion replication
    Francois Boudsocq
    Section on DNA Replication, Repair, and Mutagenesis, Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2725, USA
    J Biol Chem 279:32932-40. 2004
    ..Our studies indicate that the unique but variable LF domain of Y-family polymerases plays a major role in determining the enzymatic and biological properties of each individual Y-family member...
  40. ncbi Arginine methylation regulates DNA polymerase beta
    Nazim El-Andaloussi
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Mol Cell 22:51-62. 2006
    ..Here, a regulatory mechanism for DNA polymerase beta (Pol beta) is described...
  41. pmc XRCC1 and DNA polymerase beta in cellular protection against cytotoxic DNA single-strand breaks
    Julie K Horton
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Cell Res 18:48-63. 2008
    ..A requirement for the deoxyribose phosphate lyase activity of DNA polymerase beta (pol beta) is specific to this pathway, whereas pol beta is implicated in gap-filling during repair of many ..
  42. pmc Substrate channeling in mammalian base excision repair pathways: passing the baton
    Rajendra Prasad
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 285:40479-88. 2010
    ..Instead, flap endonuclease 1 must recognize and bind to the intermediate in competition with other molecules...
  43. pmc DNA polymerases beta and lambda mediate overlapping and independent roles in base excision repair in mouse embryonic fibroblasts
    Elena K Braithwaite
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, United States of America
    PLoS ONE 5:e12229. 2010
    ..While DNA polymerase beta (pol beta) is known to be the main polymerase in the BER pathway, various studies have implicated other DNA ..
  44. ncbi Y-family DNA polymerases in Escherichia coli
    Daniel F Jarosz
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Microbiol 15:70-7. 2007
    ..Moreover, various cellular factors can modulate their activity and mutagenic potential...
  45. ncbi DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair
    Yuan Liu
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 280:3665-74. 2005
    b>DNA polymerase beta (pol beta) and flap endonuclease 1 (FEN1) are key players in pol beta-mediated long-patch base excision repair (LP-BER)...
  46. pmc Role of the catalytic metal during polymerization by DNA polymerase lambda
    Miguel Garcia-Diaz
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 6:1333-40. 2007
    ..These data provide pre- and post-transition state information and outline in a single crystal the pathway for the phosphoryl transfer reaction carried out by DNA polymerases...
  47. pmc Structures of DNA polymerase beta with active-site mismatches suggest a transient abasic site intermediate during misincorporation
    Vinod K Batra
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mol Cell 30:315-24. 2008
    We report the crystallographic structures of DNA polymerase beta with dG-dAMPCPP and dC-dAMPCPP mismatches in the active site...
  48. ncbi 8-oxodGTP incorporation by DNA polymerase beta is modified by active-site residue Asn279
    H Miller
    Department of Pharmacological Sciences, SUNY Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 39:1029-33. 2000
    ..of 8-oxo-7,8-dihydrodeoxyguanine (8-oxodG), we performed steady-state kinetic analyses using wild-type DNA polymerase beta (pol beta) and two active-site mutants...
  49. pmc Error-prone DNA polymerase IV is controlled by the stress-response sigma factor, RpoS, in Escherichia coli
    Jill C Layton
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Mol Microbiol 50:549-61. 2003
    ..Even in cells missing LexA, the repressor of dinB, maximum Pol IV expression required RpoS. These results suggest that induction of Pol IV is part of a cellular response to starvation and other stresses...
  50. pmc UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB
    Veronica G Godoy
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 28:1058-70. 2007
    ..Together, these observations indicate that proteins like RecA and UmuD(2) may be responsible for managing the mutagenic potential of DinB orthologs throughout evolution...
  51. pmc Trinucleotide repeat deletion via a unique hairpin bypass by DNA polymerase β and alternate flap cleavage by flap endonuclease 1
    Meng Xu
    Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA
    Nucleic Acids Res 41:1684-97. 2013
    ..Our results provide new insight into the role of BER in modulating genome stability that is associated with human diseases...
  52. ncbi A role for polymerase eta in the cellular tolerance to cisplatin-induced damage
    Mark R Albertella
    KuDOS Pharmaceuticals Ltd, Cambridge Science Park, Cambridge, UK
    Cancer Res 65:9799-806. 2005
    ..Together, these data show that pol eta represents an important determinant of cellular responses to cisplatin, which could have implications for acquired or intrinsic resistance to this key chemotherapeutic agent...
  53. ncbi Threonine 79 is a hinge residue that governs the fidelity of DNA polymerase beta by helping to position the DNA within the active site
    Mausumi Maitra
    Department of Therapeutic Radiology and Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 277:35550-60. 2002
    b>DNA polymerase beta (pol beta) is an ideal system for studying the role of its different amino acid residues in the fidelity of DNA synthesis. In this study, the T79S variant of pol beta was identified using an in vivo genetic screen...
  54. ncbi Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen
    Padmini S Kedar
    Laboratory of Structural Biology, NIEHS National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 277:31115-23. 2002
    ..The results indicate that at least one of the PIM-like sequences in beta-polymerase appears to be a functional PIM and was required in the interaction between beta-polymerase and PCNA...
  55. ncbi Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site
    Robert J Kokoska
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:19633-8. 2002
    ..These results have implications for mutagenesis resulting from DNA synthesis by Y family polymerases...
  56. pmc Architecture of the DNA polymerase B-proliferating cell nuclear antigen (PCNA)-DNA ternary complex
    Kouta Mayanagi
    Medical Institute of Bioregulation, Kyushu University and Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, 3 1 1 Maidashi, Higashi ku, Fukuoka shi, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 108:1845-9. 2011
    ....
  57. pmc Systematic biochemical analysis of somatic missense mutations in DNA polymerase β found in prostate cancer reveal alteration of enzymatic function
    Chang Long An
    Department of Epidemiology and Tulane Cancer Center, Tulane University, New Orleans, Louisiana 70112, USA
    Hum Mutat 32:415-23. 2011
    ..Furthermore, the availability of detailed structural information for pol β allows understanding of the potential mechanistic effects of these mutants on polymerase function...
  58. pmc N-methylpurine DNA glycosylase and DNA polymerase beta modulate BER inhibitor potentiation of glioma cells to temozolomide
    Jiang bo Tang
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Neuro Oncol 13:471-86. 2011
    ....
  59. ncbi Local deformations revealed by dynamics simulations of DNA polymerase Beta with DNA mismatches at the primer terminus
    Linjing Yang
    Department of Chemistry and Courant Institute of Mathematical Sciences, New York University and the Howard Hughes Medical Institute, 251 Mercer Street, 10012, New York, NY, USA
    J Mol Biol 321:459-78. 2002
    Nanosecond dynamics simulations for DNA polymerase beta (pol beta)/DNA complexes with three mismatched base-pairs, namely GG, CA, or CC (primer/template) at the DNA polymerase active site, are performed to investigate the mechanism of ..
  60. pmc Human base excision repair enzymes apurinic/apyrimidinic endonuclease1 (APE1), DNA polymerase beta and poly(ADP-ribose) polymerase 1: interplay between strand-displacement DNA synthesis and proofreading exonuclease activity
    Maria V Sukhanova
    Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences Prospect Lavrentieva 8, 630090, Novosibirsk, Russia
    Nucleic Acids Res 33:1222-9. 2005
    ..We examined interactions between base excision repair (BER) DNA intermediates and purified human BER enzymes, DNA polymerase beta (pol beta), apurinic/apyrimidinic endonuclease (APE1) and poly(ADP-ribose) polymerase-1 (PARP-1)...
  61. ncbi Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication
    H Ling
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 107:91-102. 2001
    ..Dpo4 is also captured in the crystal translocating two template bases to the active site at once, suggesting a possible mechanism for bypassing thymine dimers...
  62. pmc The Asp285 variant of DNA polymerase beta extends mispaired primer termini via increased nucleotide binding
    DREW L MURPHY
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Biochemistry 47:8048-57. 2008
    ....
  63. ncbi A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining
    Stephanie A Nick McElhinny
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Cell 19:357-66. 2005
    ..This apparent gradient of template dependence is largely attributable to a small structural element that is present but different in all three polymerases...
  64. pmc Mechanism of template-independent nucleotide incorporation catalyzed by a template-dependent DNA polymerase
    Kevin A Fiala
    Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 365:590-602. 2007
    ..This unprecedented base-stacking pattern can be applied to subsequent blunt-end additions only if all incorporated dAMPs are extrahelical, leading to predominantly single non-templated dATP incorporation...
  65. pmc NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells
    Aditi Das
    Department of Biochemistry and Molecular Biology, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Route 1079, Galveston, TX 77555, USA
    DNA Repair (Amst) 5:1439-48. 2006
    ..We have also observed stable interaction between NEIL2 and other BER proteins DNA polymerase beta (Pol beta), DNA ligase IIIalpha (Lig IIIalpha) and XRCC1...
  66. ncbi Transcriptional upregulation of DNA polymerase beta by TEIF
    Yuanjun Zhao
    Department of Pathology, Health Science Center, Peking University, Beijing 100083, China
    Biochem Biophys Res Commun 333:908-16. 2005
    The overexpression of DNA polymerase beta (beta-pol) has been identified in lots of human cancers, but the mechanism has seldom been investigated...
  67. ncbi The D246V mutant of DNA polymerase beta misincorporates nucleotides: evidence for a role for the flexible loop in DNA positioning within the active site
    Shibani Dalal
    Department of Therapeutic Radiology and Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 279:577-84. 2004
    b>DNA polymerase beta, a member of the X family of DNA polymerases, is known to be involved in base excision repair...
  68. ncbi Snapshots of a Y-family DNA polymerase in replication: substrate-induced conformational transitions and implications for fidelity of Dpo4
    Jimson H Wong
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
    J Mol Biol 379:317-30. 2008
    ..Together, the global and local conformational transitions observed in our study provide a structural basis for the distinct kinetic steps of a catalytic cycle of DNA polymerization performed by a Y-family polymerase...
  69. ncbi Telomere repeat binding factor 2 interacts with base excision repair proteins and stimulates DNA synthesis by DNA polymerase beta
    Meltem Muftuoglu
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, Maryland, USA
    Cancer Res 66:113-24. 2006
    ..2 (TRF2) and proteins involved in BER of oxidized bases in vitro, we found that TRF2 physically bound DNA polymerase beta (Pol beta) and flap endonuclease 1 (FEN-1)...
  70. pmc Instability of CTG repeats is governed by the position of a DNA base lesion through base excision repair
    Yanhao Lai
    Department of Chemistry and Biochemistry, Florida International University, Miami, Florida, USA
    PLoS ONE 8:e56960. 2013
    ..Our study indicates that the position of a DNA base lesion governs whether TNR is expanded or deleted through BER...
  71. ncbi Polymorphisms of the DNA polymerase beta gene in breast cancer
    Tomasz Sliwinski
    Department of Molecular Genetics, University of Lodz, Banacha 12 16, Lodz, 90 237, Poland
    Breast Cancer Res Treat 103:161-6. 2007
    b>DNA polymerase beta (Polbeta) provides most of the gap-filling synthesis at apurinic/apyrimidine sites of damaged DNA in the base excision repair pathway...
  72. pmc Molecular insights into DNA polymerase deterrents for ribonucleotide insertion
    Nisha A Cavanaugh
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    J Biol Chem 286:31650-60. 2011
    ..Our results support a model where DNA polymerase β utilizes two strategies, steric and geometric, with a single protein residue to deter ribonucleotide insertion...
  73. ncbi A role for DNA polymerase beta in mutagenic UV lesion bypass
    Laurence Servant
    Group Genetic instability and cancer at the Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 31077 Toulouse Cedex 4, France
    J Biol Chem 277:50046-53. 2002
    We report here that DNA polymerase beta (pol beta), the base excision repair polymerase, is highly expressed in human melanoma tissues, known to be associated with UV radiation exposure...
  74. ncbi Cells deficient in the base excision repair protein, DNA polymerase beta, are hypersensitive to oxaliplatin chemotherapy
    J Yang
    Cancer Research UK Medical Research Council Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford, Oxon OX37DQ, UK
    Oncogene 29:463-8. 2010
    A significant proportion of human cancers overexpress DNA polymerase beta (Pol beta), the major DNA polymerase involved in base excision repair...
  75. ncbi De novo DNA synthesis by human DNA polymerase lambda, DNA polymerase mu and terminal deoxyribonucleotidyl transferase
    Kristijan Ramadan
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057, Zurich, Switzerland
    J Mol Biol 339:395-404. 2004
    ..This novel catalytic activity might be related to the proposed functions of these three pol X family members in DNA repair and DNA recombination...
  76. ncbi Efficiency of correct nucleotide insertion governs DNA polymerase fidelity
    William A Beard
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:47393-8. 2002
    ..An analysis of the efficiency of dNTP (correct and incorrect) insertion for a low fidelity mutant of DNA polymerase beta (R283A) and exonuclease-deficient DNA polymerases from five families derived from a variety of biological ..
  77. pmc Folate deficiency provides protection against colon carcinogenesis in DNA polymerase beta haploinsufficient mice
    Lisa F Ventrella-Lucente
    Department of Nutrition and Food, Science College of Liberal Arts and Sciences, School of Medicine, Wayne State University, Detroit, Michigan 48202, USA
    J Biol Chem 285:19246-58. 2010
    Aging and DNA polymerase beta deficiency (beta-pol(+/-)) interact to accelerate the development of malignant lymphomas and adenocarcinoma and increase tumor bearing load in mice...
  78. ncbi Marine sesquiterpenoids that inhibit the lyase activity of DNA polymerase beta
    Shugeng Cao
    Department of Chemistry, M C 0212, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA
    J Nat Prod 67:1716-8. 2004
    ..The structure of the new compound 1 was determined on the basis of spectroscopic methods and by conversion of dictyoceratin-C (2) to 1...
  79. pmc The E295K DNA polymerase beta gastric cancer-associated variant interferes with base excision repair and induces cellular transformation
    Tieming Lang
    Department of Therapeutic Radiology, Yale University School of Medicine, 15 York Street, P O Box 20840, New Haven, CT 06520, USA
    Mol Cell Biol 27:5587-96. 2007
    ..The results indicate that base excision repair is critical for maintaining genome stability and could therefore be a tumor suppressor mechanism...
  80. ncbi Genetic interaction between DNA polymerase beta and DNA-PKcs in embryogenesis and neurogenesis
    N Niimi
    Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, 641 12 Maioka cho, Totsuka ku, Yokohama 244 0813, Japan
    Cell Death Differ 12:184-91. 2005
    b>DNA polymerase beta (Polbeta) has been implicated in base excision repair. Polbeta knockout mice exhibit apoptosis in postmitotic neuronal cells and die at birth...
  81. pmc DNA polymerase beta substrate specificity: side chain modulation of the "A-rule"
    William A Beard
    Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 284:31680-9. 2009
    ..Thus, dATP insertion opposite an abasic site and dATP misinsertions have common features...
  82. ncbi DNA repair in aging rat neurons
    K Subba Rao
    ICMR Advanced Center for Research on Aging and Brain, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, Andhra Pradesh 500 046, India
    Neuroscience 145:1330-40. 2007
    ..The extension activity could be restored by supplementing the neuronal extracts with pure DNA polymerase beta (pol beta) while the restoration of gap repair needed the addition of both pol beta and DNA ligase...
  83. ncbi Roles of chromosomal and episomal dinB genes encoding DNA pol IV in targeted and untargeted mutagenesis in Escherichia coli
    S R Kim
    Mol Genet Genomics 266:207-15. 2001
    ..These results suggest that DNA pol IV efficiently mediates targeted mutagenesis by 4-NQO, and that the cellular levels of expression substantially affect targeted and untargeted mutagenesis...
  84. pmc Yeast Tdp1 regulates the fidelity of nonhomologous end joining
    Karim Bahmed
    Molecular Pharmacology Department, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 107:4057-62. 2010
    ....
  85. pmc Base excision repair in early zebrafish development: evidence for DNA polymerase switching and standby AP endonuclease activity
    Sean Fortier
    Department of Biology, Northeastern University, Boston, Massachusetts 02115, USA
    Biochemistry 48:5396-404. 2009
    ..The switch to normal, adult BER occurs fully when the embryos hatch from the chorionic membrane and encounter normal oxidative stress...
  86. ncbi DNA polymerase lambda from calf thymus preferentially replicates damaged DNA
    Kristijan Ramadan
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057, Zurich, Switzerland
    J Biol Chem 277:18454-8. 2002
    ..homogeneity and identified as a 67-kDa polypeptide that cross-reacted with monoclonal antibodies against DNA polymerase beta and polyclonal antibodies against DNA polymerase lambda...
  87. pmc A survey of splice variants of the human hypoxanthine phosphoribosyl transferase and DNA polymerase beta genes: products of alternative or aberrant splicing?
    Adonis Skandalis
    Department of Biology, Brock University, St Catharines, Ontario L2S 3A1, Canada
    Nucleic Acids Res 32:6557-64. 2004
    ..and quantified the aberrant splice variants in the human hypoxanthine phosphoribosyl transferase (HPRT) and DNA polymerase beta (POLB) in the presence and the absence of the Nonsense Mediated Decay (NMD) pathway, which removes ..
  88. pmc Template strand scrunching during DNA gap repair synthesis by human polymerase lambda
    Miguel Garcia-Diaz
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, North Carolina, USA
    Nat Struct Mol Biol 16:967-72. 2009
    ..Thus, akin to scrunching by RNA polymerase during transcription initiation, scrunching occurs during gap filling DNA synthesis associated with DNA repair...
  89. ncbi Distinct beta-clamp interactions govern the activities of the Y family PolIV DNA polymerase
    Jérôme Wagner
    CNRS FRE3211, ESBS, Universite de Strasbourg, BP 10413, 67412 Strasbourg Cedex, France
    Mol Microbiol 74:1143-51. 2009
    ..This discloses two independent modes of action for PolIV and, in turn, uncovers a novel way by which the cell may regulate the potentially deleterious effect of such low fidelity polymerases during replication...
  90. pmc Global conformational dynamics of a Y-family DNA polymerase during catalysis
    Cuiling Xu
    Department of Biochemistry, The Ohio State University, Columbus, Ohio, USA
    PLoS Biol 7:e1000225. 2009
    ..Collectively, the conformational dynamics of Dpo4 offer insights into how the inter-domain movements are related to enzymatic function and their concerted interactions with other proteins at the replication fork...
  91. pmc Replication protein A and proliferating cell nuclear antigen coordinate DNA polymerase selection in 8-oxo-guanine repair
    Giovanni Maga
    Institute of Molecular Genetics, Consiglio Nazionale delle Ricerche, via Abbiategrasso 207, I 27100 Pavia, Italy
    Proc Natl Acad Sci U S A 105:20689-94. 2008
    ....
  92. ncbi Mechanism of DNA polymerization catalyzed by Sulfolobus solfataricus P2 DNA polymerase IV
    Kevin A Fiala
    Department of Biochemistry, Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 43:2116-25. 2004
    ..The structural basis for both the protein conformational change and the low processivity of Dpo4 was discussed...
  93. pmc Long patch base excision repair proceeds via coordinated stimulation of the multienzyme DNA repair complex
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 284:15158-72. 2009
    ..repair sub-pathway (LP-BER), including DNA glycosylases, apurinic/apyrimidinic endonuclease 1 (APE1), DNA polymerase beta (pol beta), flap endonuclease 1 (FEN1), and DNA ligase I (LigI)...
  94. ncbi Properties and functions of Escherichia coli: Pol IV and Pol V
    Robert P Fuchs
    Cancerogenese et Mutagenese Moleculaire et Structurale, CNRS ESBS, 67400 Strasbourg, France
    Adv Protein Chem 69:229-64. 2004
    ..In fact, despite extensive knowledge of the individual enzymatic properties of all five E. coli DNA polymerases, much more work is needed to understand how their activities are orchestrated within a living cell...
  95. ncbi A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining
    Hui Min Tseng
    Department of Molecular Medicine and Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245 3207, USA
    J Biol Chem 277:45630-7. 2002
    ....
  96. pmc Human methyl purine DNA glycosylase and DNA polymerase beta expression collectively predict sensitivity to temozolomide
    Ram N Trivedi
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213 1863, USA
    Mol Pharmacol 74:505-16. 2008
    ..In the present study, we show that both elevated MPG expression and short hairpin RNA-mediated loss of DNA polymerase beta (Pol beta) expression in human breast cancer cells increases cellular sensitivity to temozolomide...
  97. pmc Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair
    Kiran N Mahajan
    Lineberger Comprehensive Cancer Center, Mason Farm Road, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 22:5194-202. 2002
    ..In contrast, the related mammalian DNA polymerase beta does not form a complex with Ku and XRCC4-ligase IV and is less effective than pol mu in facilitating ..
  98. ncbi Structural insights into the generation of single-base deletions by the Y family DNA polymerase dbh
    Ryan C Wilson
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, The State University of New York at Albany, Albany, NY 12201 0509, USA
    Mol Cell 29:767-79. 2008
    ....
  99. pmc Distinct energetics and closing pathways for DNA polymerase beta with 8-oxoG template and different incoming nucleotides
    Yanli Wang
    Department of Chemistry and Courant Institute of Mathematical Sciences, New York University, 251 Mercer Street, New York, NY 10012, USA
    BMC Struct Biol 7:7. 2007
    ..8-oxoG) is a common oxidative lesion frequently encountered by DNA polymerases such as the repair enzyme DNA polymerase beta (pol beta)...
  100. ncbi Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway
    T E Wilson
    Department of Pathology, Division of Laboratory Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:23599-609. 1999
    ..We transformed yeast with a series of linearized plasmids to examine the role of Pol4 (Pol IV, DNA polymerase beta) in repair at a variety of end configurations...
  101. ncbi CHIP-mediated degradation and DNA damage-dependent stabilization regulate base excision repair proteins
    Jason L Parsons
    Medical Research Council Radiation Oncology and Biology Unit, University of Oxford, Oxfordshire OX1 3QU, UK
    Mol Cell 29:477-87. 2008
    ..The scaffold protein XRCC1, DNA polymerase beta, and DNA ligase IIIalpha play pivotal roles in BER...

Research Grants54

  1. MECHANISM OF BASE EXCISION REPAIR IN HIGHER EUKARYOTES
    Yoshihiro Matsumoto; Fiscal Year: 2003
    ..The first specific aim is to elucidate the excision mechanism of a 5'- incised AP site by DNA polymerase beta (pol beta) from structural and functional perspectives...
  2. DNA Polymerase Beta and Gastric Cancer
    DAWIT KIDANE MULAT; Fiscal Year: 2013
    ..Those processes require a large number of proteins including DNA polymerase beta (Pol ?)...
  3. Base Excision Repair and Autoimmunity
    Joann B Sweasy; Fiscal Year: 2013
    ..We have recently generated base excision repair compromised mice carrying the Y265C DNA polymerase beta variant, and found that they exhibit symptoms of autoimmune disease...
  4. Characterization of a DNA polymerase Beta deficient mouse
    Joann B Sweasy; Fiscal Year: 2013
    ..We have recently shown that DNA polymerase beta (Pol beta) is critical for double-strand break (DSB) repair, in addition to its role in base excision ..
  5. DNA POLYMERASE BETA AND MUTAGENESIS
    Joann B Sweasy; Fiscal Year: 2013
    We have recently shown that DNA polymerase beta (Pol ss) plays a key role in maintaining genomic stability and preventing cellular transformation. Approximately 30% of human tumors studied express Pol ss variants...
  6. MOLECULAR BASIS OF VIRAL AND CELLULAR TRANSFORMATION
    DANIEL C DIMAIO; Fiscal Year: 2013
    ..Dr. Sweasy will study the ability of cancer-associated DNA polymerase beta mutants to induce cellular transformation, with a focus on truncation mutants, animal models of ..
  7. DNA Polymerase Fidelity Mechanisms: Theory and Experiment
    MYRON GOODMAN; Fiscal Year: 2012
    ..the molecular basis of DNA polymerase accuracy, relating theory to experiment and vice versa, using human DNA polymerase beta as a model system...
  8. DNA Polymerase Beta Variants and Cancer
    Joann B Sweasy; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Polymerase Beta Variants and Cancer. Abstract: DNA polymerase beta functions in base excision repair (BER) which is important for the repair of endogenous DNA damage and damage caused by agents such ..
  9. HYDROXYL RADICAL BIOLOGY BY CEREBRAL ISCHEMIA
    Philip Liu; Fiscal Year: 1999
    ..This model will be suitable for exploring pathobiological effect of oxygen stress, and for testing drugs that reduce or abolish the damaging effect of hydroxyl radical after stroke. ..
  10. Dynamic Characterization of Cancer-Associated Variants of DNA Polymerase Beta
    ELIZABETH MARGARET MOSCATO-GOODPASTER; Fiscal Year: 2013
    ..One of the crucial enzymes in this process is DNA Polymerase beta (Pol beta), which is responsible for the fidelity-ensuring step of inserting the appropriate nucleotide ..
  11. RNA-Mediated Silencing: Mechanisms and Biological Roles in Chlamydomonas
    Heriberto Cerutti; Fiscal Year: 2009
    ..One group encodes factors that appear to be directly involved in RNAi: Mut68p, a putative DNA polymerase beta-like nucleotidyltransferase;MutTOp, a homolog of the vasa intronic gene product;and Mut91p, a novel but ..
  12. BIOPHYSICAL AND SCANNING FORCE MICROSCOPY STUDIES
    DOROTHY ERIE; Fiscal Year: 2001
    ..b>DNA polymerase beta subsequently extends the DNA chain by one nucleotide and removes the dRP site with its dRP lyase activity, ..
  13. HUMAN DNA REPAIR ENZYMES FOR REDOX AND ALKYLATION DAMAGE
    BRUCE F DEMPLE; Fiscal Year: 2010
    ..generate abasic sites, which are further processed in several steps dependent on the Ape1 endonuclease and DNA polymerase beta. Our long-term goal has been to ascertain the biological functions of Ape1 protein as a central player in ..
  14. Base excision repair in zebrafish development
    PHYLLIS STRAUSS; Fiscal Year: 2007
    ..These studies will help define the role of BER in embryogenesis and open a new field of inquiry. [unreadable] [unreadable] [unreadable]..
  15. MECHANISMS OF FIDELITY AND MUTAGENESIS
    Thomas Spratt; Fiscal Year: 2005
    ..Two polymerases will be studied, DNA polymerase I of E. coli and mammalian DNA polymerase beta. The biochemical mechanisms of DNA synthesis by these proteins have been well studied, thus providing a ..
  16. DNA POLYMERASES IN NORMAL AND CANCER CELLS
    LUCY CHANG; Fiscal Year: 1991
    ..4. To compare the structure of DNA polymerases and DNA primase in normal and transformed cells at protein, RNA and genomic levels...
  17. Transcription coupled repair of oxidative DNA damage
    BRETT HALTIWANGER; Fiscal Year: 2003
    ..And (2) that transcription-coupled BER of oxidative damage which requires XPG proceeds by a DNA polymerase beta independent pathway, with the possible involvement of XPG in the selection of the repair patch pathway...
  18. AN ENVIRONMENTALLY RELEVANT DNA POL BETA VARIANT
    Sipra Banerjee; Fiscal Year: 2003
    For the elucidation of functionally important DNA polymerase beta (polbeta) variant, we propose experiments to test a hypothesis of whether a defective repair gene, such as polbeta, has a contributory role in the increased disease risks ..
  19. HUMAN XRCC1 PROTEIN IN DNA REPAIR AND RECOMBINATION
    MICHAEL THELEN; Fiscal Year: 2001
    ..XRCC1 protein was later shown to interact with several BER participants, namely DNA Ligase III, DNA Polymerase Beta, and Poly(ADP-Ribose) Polymerase...
  20. Folate Deficiency and Susceptibility to GI Disease
    AHMAD HEYDARI; Fiscal Year: 2003
    ..The model of BER deficiency used in these studies is the DNA polymerase Beta heterozygous knockout mouse...
  21. DEVELOPMENT OF A FOS RIBOZYME
    Kevin Scanlon; Fiscal Year: 1993
    ..e. dTMP synthase, DNA polymerase Beta or c-Hras). A wide spectrum of agents can elicit increased c-fos gene expression in cells...
  22. ENZYMOLOGY AND GENETICS OF DROSOPHILA DNA REPAIR
    James Boyd; Fiscal Year: 1990
    ..These enzymes include DNA polymerase Beta, DNA polymerase Gamma, two ATP-dependent deoxyribonucleases, a rec A-like protein, and a damage-specific ..
  23. NMR STRUCTURAL OF DNA POLYMERASE B
    Mark Maciejewski; Fiscal Year: 2003
    Mammalian DNA polymerase beta catalyzes abasic site excision and nucleotidyl transfer in DNA repair and replication in mammalian cells. The enzyme is central to the repair of environmentally induced DNA damage in humans...
  24. ELABORATION OF MODIFIED PROTEINS USING MISACYLATED TRNAS
    SIDNEY HECHT; Fiscal Year: 2007
    ..of requested support, we propose to prepare and study analogues of firefly luciferase, MAP kinase ERK2, and DNA polymerase beta. Also of interest is the exploitation of a new method for introducing a broader range of amino acid ..
  25. Progestogen Inhibition of Ovarian Cancer Cell Metastasis
    William Murdoch; Fiscal Year: 2003
    ....
  26. The Effect of Mre11/Rad50on Fidelity of Synthesis by DNA Polymerase Beta
    Drew Murphy; Fiscal Year: 2008
    ..b>DNA polymerase Beta (pol-beta) is the main polymerase involved in base excision repair (BER), which is a major mechanism of ..
  27. DNA Polymerase Beta and Breast Cancer
    Joann Sweasy; Fiscal Year: 2008
    ..studies suggest that at least thirty percent of the 189 tumors examined to date express variants of DNA polymerase beta (Pol beta) gene and none of these mutations are common polymorphisms...
  28. DNA POLYMERASE BETA AND MEIOSIS
    Joann Sweasy; Fiscal Year: 2004
    ..Testicular proteins that interact with Pol-B in two hybrid screens will be characterized to make conclusions about their function. ..
  29. DNA polymerase beta and colon cancer
    Joann Sweasy; Fiscal Year: 2007
    ..These variants will be characterized in genetic and biochemical assays to determine if they have phenotypes that are consistent with cancer etiology. [unreadable] [unreadable]..
  30. Annual Midwest DNA Repair Symposium
    ROBERT SOBOL; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  31. Regulation of DNA Replication and Repair
    Mark Sutton; Fiscal Year: 2008
    ..abstract_text> ..
  32. Regulation of BER Gene Expression by Folate Deficiency
    DIANE CABELOF; Fiscal Year: 2006
    ..The loss of the BER response to DNA damage when folate is deficient would establish a mechanism by which diet can alter susceptibility to environmental exposure. [unreadable] [unreadable] [unreadable]..
  33. MUTATIONAL MECHANISMS OF REPETITIVE DNA IN HUMAN CELLS
    Kristin Eckert; Fiscal Year: 2001
    ..These targets will be used as DNA templates during in vitro DNA synthesis catalyzed by human DNA polymerase beta and the calf thymus polymerase alpha-primase complex...
  34. DNA replication, DNA repair and microsatellite stability
    Kristin Eckert; Fiscal Year: 2009
    ..Microsatellite allele lengths can directly affect gene expression. As microsatellites are polymorphic in human populations, this effect on gene regulation may be an important factor contributing to individual cancer risk. ..
  35. ENZYME INTERMEDIATE STRUCTURES BY NMR
    Michael Smerdon; Fiscal Year: 2002
    ..This might enable the rational of antibacterial agents in the future. ..
  36. Bacterial cell cycle control
    SUSAN THOMAS LOVETT; Fiscal Year: 2010
    ..LAY DESCRIPTION: We will study a protein present in all bacteria that appears to control their growth. This will provide important missing information about how bacteria divide and may provide a target for antibiotics. ..
  37. Regulatory Functions of APE1 - An Essential Repair Protein
    Sankar Mitra; Fiscal Year: 2010
    ..Furthermore, our studies should help identify APE1-regulated key signaling pathways in tumor induction and promotion which could be potential targets for cancer therapy. ..
  38. Effects of Formamidopyrimidine Lesions on DNA
    Marc Greenberg; Fiscal Year: 2006
    ..abstract_text> ..
  39. INDEPENDENT SCIENTIST AWARD (NIEHS)
    ASHIS BASU; Fiscal Year: 2002
    ..Another important aim is to understand the mechanism of how the repair proteins protect cells against the toxic and mutagenic properties of these DNA damaging agents. ..
  40. FUNCTION OF PCNA IN CELLULAR DNA REPAIR
    Yoshihiro Matsumoto; Fiscal Year: 2001
    ..In the past, base excision repair was thought to proceed exclusively by a DNA polymerase beta (pol beta)-dependent pathway...
  41. GERM CELLS--DNA REPAIR, MUTATION, & ENVIRONMENTAL AGENTS
    CHRISTI WALTER; Fiscal Year: 2001
    ..This study will advance an understanding of the fundamental mechanisms involved in mutagenesis in germ cells. ..
  42. Database-driven software for biological NMR analysis
    Michael Gryk; Fiscal Year: 2006
    ..Documentation of the application will be completed in the R33 phase. ..
  43. Ring-Opened and Tandem DNA Damages
    Ashis K Basu; Fiscal Year: 2010
    ..g., isogenic mouse embryonic fibroblasts and cells that express modulated levels of DNA polymerases encoded by REV1, REV3, RAD18, etc. genes). We hope to contribute toward a deeper insight of the effects of oxidative DNA damages. ..
  44. NUCLEASES IN DNA REPAIR AND RECOMBINATION
    SUSAN LOVETT; Fiscal Year: 2006
    ..We will extend genetic analysis of radC to determine what role it may play in repair and recombination. ..
  45. NO-MEDIATED MODIFICATION OF NMDA RECEPTOR DURING HYPOXIA
    Om Mishra; Fiscal Year: 2006
    ..abstract_text> ..
  46. Mechanisms of Nonhomologous Repair of Damaged DNA
    Sang Eun Lee; Fiscal Year: 2005
    ..Furthermore, since DSB repair by NHEJ is remarkably conserved from yeast to humans, these studies will help to dissect the similar pathways in humans. ..
  47. Error Prone DNA Synthesis and Oncogene Mutagensis
    Bernard Strauss; Fiscal Year: 2004
    ..Blocking the action of the error-prone polymerases should not stop replication. However, mutational cascades, such as those leading to tumor drug resistance, should be inhibited by such a block. ..
  48. Base Excision Repair, Genetic Integrity & Health Span
    CHRISTI WALTER; Fiscal Year: 2008
    ..The overall hypothesis to be tested is that modulation of DNA polymerase beta abundance/activity will have differential effects among tissues relative to age...
  49. Germ Cell Aging
    CHRISTI WALTER; Fiscal Year: 2007
    ..This study will advance our understanding of the fundamental mechanisms involved in male germline integrity. ..
  50. NMR STRUCTURAL AND BIOPHYSICAL STUDIES OF XRCC1
    Michael Gryk; Fiscal Year: 2003
    ..Backbone dynamics will be characterized and correlated with functional binding sites. The 40 kDa complex, involving the N-terminal domain of XRCC1, DNA substrate and beta-Pol, will be structurally characterized using NMR-based methods. ..
  51. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2009
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  52. NO-MEDIATED MODIFICATION OF NMDA RECEPTOR DURING HYPOXIA
    Om Mishra; Fiscal Year: 2008
    ..abstract_text> ..